LOCUS       DAOSMM010000001       179114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_46_length_179114_cov_16.262964, whole genome shotgun sequence.
ACCESSION   DAOSMM010000001 DAOSMM010000000
VERSION     DAOSMM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 179114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 179114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..179114
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46_length_179114_cov_16.262964"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="PK898_00005"
     CDS             <1..1590
                     /locus_tag="PK898_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18446.1"
                     /translation="HSGMDSMMHPDARSDFPDYNAFIYSILRLPECLIKVQRIILGQN
                     IKMFAEQGYSDIEKWEVVSARARRRYCLYNNSDTLACFITSRSDIDDLIPEITAFQIE
                     WNKFHYLLMKVPDEYLKNIDPKNETEYRFIAEAILSPVEDLNRLYTIWGKKTGTWLLE
                     MKRRVSELRVKLLDSSSIQYSRSANSWMKNILQEMPDIVDRPIYFVSSNTHSITNMIS
                     GYALSKESELIDFLKKMDDQSLFHEWDLISRNKLSAKKENLLYYVLKKYQQTPAGAYT
                     VKEQQEIENEGGIFRISGYNTFDIETQIIELSKIKTENIDPRIKLDNIEKLKKSEAIL
                     FNIDYPLGMTAFNVMTKFAEEFEDVRGIYIMGKAASLNGIYGDVIIPTAVHDIHSQNT
                     YLFQNCFTASDIEPWLVYGSILDNQRAVSVFGTFLQNHRFVETLYQGGYTDIEMEAGP
                     YLSAIYEMVRPDRHPTNEFITLYNNRFDLGILHYVSDTPMSKGKNLGAGTLSYFGMDS
                     TYATSIAILRRIFTIESHFIK"
     gene            1628..2647
                     /locus_tag="PK898_00010"
     CDS             1628..2647
                     /locus_tag="PK898_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU18447.1"
                     /translation="MSQKSRFIVIILIVLLMLLIILIYGVFMNVQSGVGFLFIPSKSY
                     TPTITATQTITPTITFTPSKTLDLNLLKTNAVNTYVAVNLIQTQTQLSIATSTGTPDV
                     SVIRTEAVNRAITELTETEMVVRETEKWMPQINSKGFTEKILGKDQSIMVLIDDVSRP
                     FWIDKYEVTNASYQKCIKDGNCSIIDSNVGEEISQDTFNSLPVVNVSWNHAKDYCEWA
                     GKRLPTKDEWLEAAGVDKDFLYPWGKRVTEGMPVNANEENGKPVAADTYPEGCSFYGV
                     CNLLGNVWEWVASSNPEPDSEQQSIMGGGWRTFSGDLNNRIEGKMNKTESADDVGFRC
                     ASDIE"
     gene            complement(2733..4274)
                     /gene="malQ"
                     /locus_tag="PK898_00015"
     CDS             complement(2733..4274)
                     /gene="malQ"
                     /locus_tag="PK898_00015"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU18448.1"
                     /translation="MNLERSSGILLHPTSFPSLDGIGDLGPQAFYWVDFLAAAKCSVW
                     QILPLGPTGYGDSPYQSFSAFAGNPYLVSSTILLDEGYLTHEDLTDRPIFPESRIDYG
                     KAIQWKLKILDRVYENYKNHPGNLESEFIEFKEKNQYWLSDYSLFMAIKDTQDGHSWL
                     DWPERLRKRDQTELIDFSKKNAELIERHAFRQFLFFRQWLKLKQYANSKGITIVGDIP
                     IFVSMDSSDVWSNPDLFYLDSEGHPTVVAGVPPDYFSPTGQLWGNPLYKWPEHEKTDF
                     DWWVRRIKATLLTADLIRLDHFRGFNGYWEVPSGELTAIKGRWVEGAGSALLKKLKDE
                     LGDLPIIAEDLGEITQDVVDLRDSFGLPGMKILQFAFSDDARNLFLPHNYIQNCIAYT
                     GTHDNDTAIGWYQTAPESEKDFARRYLMSNGEDIAWKMVRCVWSSVAVYAIAPLQDIL
                     QQGPEARMNFPGKAEGNWCYQFKPSDLTPELAFRIRELNTIFNRIPEINRIPIQRPII
                     KYMKP"
     gene            complement(4303..5775)
                     /locus_tag="PK898_00020"
     CDS             complement(4303..5775)
                     /locus_tag="PK898_00020"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOU18449.1"
                     /translation="MAKKLNILFLISEADPLIKVGGLGDVGGTLPIALKKLPKEKIDR
                     TEIDIRVVIPYHDQIKQKNWPTEWLLNYKIQSRRGFKDVYLSKALNTEVPIYLIDGMP
                     IRNTGVYSSNTHRDLEKYAFFSVAAFELCRKINWKPDIIHAHDWHTALTVKLLAQNRS
                     FDPFFSRTKSLYTIHNLPYSGGDHSDILDLYDIRPAFDLDFPDYLRNLPMAIGLDSAD
                     HISTVSKTYAEEITTEAFGCGYQTFLRNHKYKLSGILNGIMMDQWNPATDHSVYMNFD
                     VNHFENRFANKQHLQADFNLKQDQNIPLFVMVGRMTFQKGIDLAIQALNQMNDLNWQA
                     IFLGTGEEAVQRACIDLENQFPDRVRAIIRFDEKLSRKMYAGGDMLLMPSRYEPCGIT
                     QMISMLYGCIPIACATGGLKDTIKDPFDFPNDYTGFLFENADIGGTEWAMRRAIEYFY
                     DRENWKQIQIRAMNQDFSWDKQALEYFSLYNSLLSIDTKS"
     gene            complement(5818..6969)
                     /locus_tag="PK898_00025"
     CDS             complement(5818..6969)
                     /locus_tag="PK898_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOU18450.1"
                     /translation="MPNVEIIAIGSELLLGETQDTNTFYLLRKLRDLGFDVFRTTLIG
                     DNPKRIASTIQESLERSDIVITTGGLGPTVDDPTRDAVAMAFGCKTEFKEELWMDIRN
                     YFDKMGRSVSENNIRQAYIPELAIAIPNPVGTAPAFYVYKDSKLVLSLPGVPAEMRYL
                     IEHSGTDLLLKYFPTQDSILVKTLHTFGLGESVMDEFVGDLEKSSNPTLGLSAKQGII
                     DLRITAKGKTKAEAERLLNDFETEVRNRLGDNIFGVDDETLPEVVNRMITRNYVKLNV
                     IEYGTGGAITKALSPSSIQTSSVQEMPDMSFSFRDHVQNIKSKMEIDSDSPLLICAIF
                     PISDSFIRSEIYLRTNRKEEYAERNYNSLTFSVDQTVLSAFEMIRKAII"
     assembly_gap    7395..7494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7807..7879)
                     /locus_tag="PK898_00030"
     tRNA            complement(7807..7879)
                     /locus_tag="PK898_00030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7844..7846),aa:Arg,seq:tct)
     gene            complement(7932..9038)
                     /locus_tag="PK898_00035"
     CDS             complement(7932..9038)
                     /locus_tag="PK898_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU18451.1"
                     /translation="MEKSKVYFTNLRTITMVNNRQSKLEKLIRTAGIGQIDFDHKYAA
                     IKMHFGEPGNLSYLRPNYAKTVADVVKSLGGKPFLVDCNTLYVGRRKNALDHIDSAYE
                     NGFSPFSTGCHILIGDGLKGTDETLVPINGDYVKEAKIGTAVMDADIFISLTHFKGHE
                     NAGYGGTLKNIGMGCGSRAGKMEMHSSGKPFVDQEKCIGCGKCVKICAWGAPKVTNKK
                     SQIDHDKCVGCGRCIAVCPVDAIEPEWGQSNIILDRKIAEYAYAVVKDRPSFHISLVI
                     DVSPNCDCHDENDLAIIPDVGMFASFDPVALDQACIDAVNQQPPNPGSWLAEAHHTGD
                     HFTDIHPDTNWKECITHAEKIGLGHAEYELIEVK"
     gene            complement(9081..10019)
                     /locus_tag="PK898_00040"
     CDS             complement(9081..10019)
                     /locus_tag="PK898_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HOU18452.1"
                     /translation="MAKEEIQLVLVSGFMGAGKTTFLQYILNQQHHKKIGILVNEFGP
                     VGIDGKILKGSDAEIVEINNGSIFCACLKNNFVDTLISFSKKPIDLLLIENSGLGDPS
                     NFLKILSEIEPYTERKYTLKGTVCIVDSQFFLEYNEIFTPVQNQIAASNFILINKTDL
                     VDPAHLEKIHETVKKINPQAYLYDTQYGILPIEILKEHLIASDFIGETSNHDWNRPVN
                     YLISSDKTVEQDELIDFLETIKEKILRLKGFLKAPDGYWHIDMVEDFIQLTKLQNHAQ
                     LEPELVVIGKDQRDFQSLLKETWERTFKHTPNILPN"
     gene            complement(10088..11887)
                     /locus_tag="PK898_00045"
     CDS             complement(10088..11887)
                     /locus_tag="PK898_00045"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOU18453.1"
                     /translation="MQIDFEPIGKRIRINSNQSVLEAAQMAGIQITALCGGNGTCGAC
                     KIRLMSGTSTPLSDTEKKFLSEKEIKENYRLACKAFPKSDLKIFVPPESLSTSQRLQL
                     EGIKNISDSSDCIIQRTDLCLSQPTLSDPRSDLTRIKEGLSENGHLCTRCNYNILESL
                     SDSVRRFNWKFCAVQREDELVAVLPSKSRILGLAIDIGSTKIAAYLVDLEISAVLSKK
                     GITNPQISYGEDVVTRIAYADSHPHGREILQEKLITALNNLIRELCTEVGEKSEQIVE
                     IVAVCNTAIHHFFLNLPVRQLGVMPFIPAITDSINVPAGKLGLNVAPGAYVYLPNNVA
                     GYIGADHIAMLLATGIHKLSKTEMALDIGTNTEISLSHKGKIYSCSCASGPAFEGAHI
                     QNGMRASTGAIEKAQMIDGEMKVQTIDQEKAIGICGSGILDIVGSLRASGVINERGNF
                     NRQVKNVRPAEKNYEFVLVNKEDSGTDRDITVTRFDINEIILAKSAIRTGIDILLKEA
                     NLQPSDIEEFIVAGAFGTYLSVENAIRIGMFPNIPIERYRQVGNAAGTGAIDLLLSKS
                     LRTECDKLCKRINYIELTSYPDFRQILVDNMRL"
     gene            complement(11902..12600)
                     /locus_tag="PK898_00050"
     CDS             complement(11902..12600)
                     /locus_tag="PK898_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18454.1"
                     /translation="MPILTNWKITISVDDILRAQGANPNILRERSPHLITVAENALKD
                     AGELLHPFVMYEISDVIQRENERLFLANGHQISGKIVNKQLACANRILIAACTISDEL
                     EKIVSAEFHKDPSYALALDAAGIAAVDKLSSLAQKYFKQNLIPPDLKATSAFNPGMKD
                     WPVAMGQEQIFSILEKISDRIRLSDSFLMIPAKSVSFVIGIGNEVDTTTKQCDYCDKK
                     QTCYLSKAGKELIS"
     gene            complement(12634..14001)
                     /locus_tag="PK898_00055"
     CDS             complement(12634..14001)
                     /locus_tag="PK898_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU18455.1"
                     /translation="MNKKIKGKELLLRTLKHERIDGVPWVPFAGVHAGKLKGYSALEV
                     LKDEEKLFESLLEVNKVYDPDGQPIVFDLQIEAEILGCDLLWAEKTPPSIASHPLAFE
                     KSIPDKLPEATDGRLPMILNVMRRMKIAVGDHTALYGLICGPFTLASHLRGTEIFMDI
                     FQEKEFLQSLLDYCKRVCMRMIDLYVDAGMDVIALVDPLVSQISPRHFKQILADPYTE
                     LFTVIREKGVLSSFFVCGDATKSIEVMCQTLPDNISIDENVDILKAKEITDRYNICIG
                     GNIPLTTRMLLGTQQDNMKFALDLIDSIDHFNWILSPGCDMPYDTPIENTVGILQAVR
                     QPEETRKMLENYQAATYDIDSVILPDYVHLEKPLVEVFTLDSETCAACTYMWNTAKRV
                     VEELGGKADIVEYRFTKKENVARVMKMGIKNLPSIVINGELKYSSVIPNPAELRNEIL
                     KYIKS"
     gene            complement(14183..14626)
                     /locus_tag="PK898_00060"
     CDS             complement(14183..14626)
                     /locus_tag="PK898_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18456.1"
                     /translation="MNNINEGGFAKPFIKPVLSTKFHIDFDWWKENDSSWRLSLMGYL
                     CDKHKKFFESSNDNQLVDSVNSDTGEIIQVDQLQDILINHCARNDDFISGNGPLTDSV
                     FRIFLSNGNTPLDSEEISKLINRPAETILKTLSGPRVYKGIRPIL"
     gene            complement(14619..16223)
                     /locus_tag="PK898_00065"
     CDS             complement(14619..16223)
                     /locus_tag="PK898_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18457.1"
                     /translation="MNDKSVNAIVNWKDLNLSQSDLDFIYNYLLEKEVPMTSLELSKA
                     IIDEHIRLFRKELQKKEKSRGQVYRPNRDYQMGEEILFPLSDNQKAKVVNIRQGNNPD
                     LPDFNAITFLFEDGTEKEFAGSLQDHRLNSFDYYSSDSEFLLDPEEILDRFGGSISKK
                     VRQLLAQSDGLFTLADFWFPKALLSEVNLGYLNLAEAALEMEEGQPVQTRKILEAIEY
                     PMDSNDLLTEFSFNYALYKDDRFDEVGPTGKVLWVLKLLEPEDVRKKPLTLKYYGIDS
                     EKADINGYSLDHLQIHDELDPDNQITPETEIKTFDICINYPHWKAGSLPLIGSIRPIF
                     PTALETDKVIFEFVDKNKSSKFPGWVLVKENYVSGLKQWYRENHIIPGSFIRLRKIDN
                     STDVEISMIPPRSSKDWIRTAVFDSKGKIHFEIRQQPINTDFDERMCIYVENNPQLDL
                     IWDQNNKPDINFGKLVENIFKEISSDNPQGIVHFLELYAAVNMLHRCPPKPLLATLIH
                     NDHFEILDNYYFKINSKESKLETEYE"
     gene            complement(16268..17410)
                     /locus_tag="PK898_00070"
     CDS             complement(16268..17410)
                     /locus_tag="PK898_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU18458.1"
                     /translation="MQSVNLGDTLANISILSGMDVNDILKINKISSLSEVYVGSTLIL
                     VQNTNGNRYSAVDTFENDETSLEKAVLQKTNPEKLAKINRLNGSWDIAEKQLLFSTVS
                     EDLNIPVHAISPLIDQIEITNLPLMQGETQVIHLKTPYSLTLSGAILNQDLNFYQEDT
                     ASNDWYALFGVDAEQETGLTDLQLSGSNSNGESFDIRQKVVVEPGVFTYEVVSGVDAS
                     TLEDHSNEVDQATLAGITQTSPIKSWGDRMSYPVDEPCLVSGFGNRRTYNDGSYTNFH
                     SGVDFGVCTANNINIYAAADGTVLFSGDLPIHGKHTIIDHGWGVYSTYSHQSETFVTA
                     GQQVKRGDLIGLIGSTGRSVGPHLHWEIKVNGIYVNPLAWINNSFP"
     gene            complement(17707..18687)
                     /locus_tag="PK898_00075"
     CDS             complement(17707..18687)
                     /locus_tag="PK898_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOU18459.1"
                     /translation="MDASQVSIIQSIKMFIDNVSLSRSENTARTYSNGLDVFLEMLDE
                     NQIDKESSVSVITEEFVSDFSKYLKSYSPTTESLYINVFKNYLEFLAAENLSDLNMHR
                     VKLLIAQRSRKPGIRIPQFPIESIEKVLAYAETLPDKPVENDVQRLINLRDSAFLLTL
                     ADTGLRIHEICGLRRGDIDWFRNKAVIIGKGNKQAVIRFSKRSIMSMRNYLNERGVLD
                     GSGTLPLSALPVFARHDKGSGKNILPITTKTGRLIVSERVSECLGKDAVGTITPHSFR
                     HYFVTTVLHETGNLKIAQEFARHTNIAVTQRYTHLANDELDDTYQKIFDK"
     gene            18953..19291
                     /locus_tag="PK898_00080"
     CDS             18953..19291
                     /locus_tag="PK898_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOU18460.1"
                     /translation="MEKKPRVSGSTMIAPEVIETIIKMTAMETDGVSKIPAQLTHQPV
                     RIRINEEKKIDADVYVRIEYPYNVIHVGKKLQENINRVISETIGMEPGSINIHIEDID
                     YLNNSEKERS"
     gene            19303..19854
                     /gene="nusB"
                     /locus_tag="PK898_00085"
     CDS             19303..19854
                     /gene="nusB"
                     /locus_tag="PK898_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOU18461.1"
                     /translation="MSETNRELKPEDQDNHKTLDNKDNPDSSQDEIIESVVIDNIELD
                     DRTRARGVALQVLYEMDMSDHPINLILQERIQSVPLDEQQTKLVLKIVLGLQPYIHYI
                     DDFIEKYAPEWPIDQIAVIDRNIIRIAVWEFAIGEITPIKVAINESVELAKLFGSESS
                     PRFVNGVLGSLAEHIKDIQKIKS"
     gene            20044..21648
                     /gene="pckA"
                     /locus_tag="PK898_00090"
     CDS             20044..21648
                     /gene="pckA"
                     /locus_tag="PK898_00090"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOU18462.1"
                     /translation="MENVGIPGKYGLENYGIKNAGKVYWNAVPAILVEESVKRNEGVL
                     SPDGALMVNTGKYTGRSPNDKFIVDNGTEEEKHIDWGKVNVKMSPEHFNKIYDKVLGY
                     VQNRDLFVADLVAGADPVYRLPMRIITEFAWSNLFITDMLIRPSLEERIGQVPGFTII
                     CVPGLEAIPEEDGTHSGAFIIVNFAKKLVLIGGSHYGGEIKKSVFTVMNFILPLKGVM
                     TMHCSANVDKKTGDSALFFGLSGTGKTTLSSDPNRLLIGDDEHGWSDTGIFNFEGGCY
                     AKMIKLSKEHEPLIWNAVHRFGALLENVVYDPATRVIDFDSDARTENTRGAYPIHFID
                     GIVENGLGPHPTNIFMLTADATGVLPPLSKLDNNQAMYYFMSGYTSKLAGTERGITEP
                     QPNFSACFGSPFLPLHPSVYAELLGKKIHEHHANVWLLNTGWSGGPYGIGKRFSLPYT
                     RAFVTAALNGEMANVEFVKEPFFGLLIPKTCPGVPSDVLNPRNTWEDKAAYDVAAKKL
                     AAAFIENFKKYEAKTAKDIIEAGPKA"
     gene            complement(21700..22215)
                     /locus_tag="PK898_00095"
     CDS             complement(21700..22215)
                     /locus_tag="PK898_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOU18463.1"
                     /translation="MRRADREISEVEEIAKILDSIEIMRIAIFDNPYPYIIPLNFAYF
                     LSHENKITFFFHSANQGRKIELIRQNAHVGFEADKSYGVTNSESEIPCKMSTLYESVV
                     GFGRIKIISDNLEKQEGLKILINRFSKVPLSFANYDPILLDRVVILKLDVEGITGKRH
                     MIPNKLSPDIL"
     gene            complement(22227..22805)
                     /locus_tag="PK898_00100"
     CDS             complement(22227..22805)
                     /locus_tag="PK898_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18464.1"
                     /translation="MPTSGNESYPNEPNNEGKIAGFLRTDVIICFVFSWIIYSFLIQN
                     NFLPSAKLSKTAWIILVLIANFDILFYKIPILLLIVFVLFVIFPGITQKIGINNFLGA
                     LFNFLILYILFVIGKKIYKGKIVPFGFGDVLFGTVLGFALGFINGLGALSFALILSGI
                     TAGILKILKKPNFMTIPLVPFFVSGAIFYILL"
     gene            complement(22900..22971)
                     /locus_tag="PK898_00105"
     tRNA            complement(22900..22971)
                     /locus_tag="PK898_00105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22937..22939),aa:Met,seq:cat)
     gene            complement(23049..23753)
                     /gene="radC"
                     /locus_tag="PK898_00110"
     CDS             complement(23049..23753)
                     /gene="radC"
                     /locus_tag="PK898_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOU18465.1"
                     /translation="MAKEIDYVYHNRISDMEINERPRERLVKVGPGSISNYELLAILL
                     RTGTAGENVIDLSKRILNSYGGLGGLKKISFEELKNAKGIGEAKASQVLAAVELGKRL
                     AGLQEENDRLIINSSQDVFDLVSYEMSAFDHEELWVLNVDTRHRLVAIDRLYKGSLNS
                     STVRISEVFRKPITRNVAAILLVHNHPSGDPKPSAADIAVTKSVQEAGNLLEITMLDH
                     IIIGSGKYESILSYLI"
     gene            23925..24389
                     /locus_tag="PK898_00115"
     CDS             23925..24389
                     /locus_tag="PK898_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU18466.1"
                     /translation="MDIDPQILSNIVQILTTLGVAFLTALWLSILFWVFRDIRHRTRD
                     PFMIILAVILVIILPIVGVVIYWIIRPNQTIEEKYQAALEEEALLQELDKKPKCPGCG
                     RNVEPDWILCPACHTRLNKLCISCGKVLELPWNLCPYCGTPQQKTFRSEQNQ"
     gene            24437..25186
                     /locus_tag="PK898_00120"
     CDS             24437..25186
                     /locus_tag="PK898_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU18467.1"
                     /translation="MINQKGGVGKTTTAINLGSSLAALGKKVLVIDIDPQANATSSLG
                     MDKNEVRYGTYQVLIGSVPITPHIIENRDFHLSILPASPALSGAEIELIDLPNRESML
                     KNALKPIQEKFDYILIDCPPSLSLLTINALVASKDGVIIPVQCEYLALEGLGQLTNTI
                     NQVRKSFPEVNVRGVLLTMYDGRTRLALDVVNEVRKFYPDKVFESIIPRSIRVAEAPS
                     YGQPINYYSKDSSASKAYDALAREILAQDQK"
     gene            25207..26067
                     /locus_tag="PK898_00125"
     CDS             25207..26067
                     /locus_tag="PK898_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU18468.1"
                     /translation="MCAQRKGGLGRGLGALLPVSDNEISDESVRMVDVSVIQSNPHQP
                     RVDFNEDELKELTDSILEHGVIQPLIVVPEDDGKYTLIAGERRLRAAIAAGLSMVPVI
                     TRSATDQDLLEIALIENVQREDLSPLETAEAYRSLEVNFNLTHDEIAKKVGKNRVSVT
                     NTIRLLKLPEKVRNALSNRMISEGHARALLALNTEVQQLSALQTILLHNLNVRQTEEL
                     VRRMSGEKTGKEDKKKENSPEIKSIEEQLMQSIGTRVSLHPGKKGGTIHIHYYSDEEL
                     EDLVDRFTGR"
     gene            26071..26928
                     /locus_tag="PK898_00130"
     CDS             26071..26928
                     /locus_tag="PK898_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU18469.1"
                     /translation="MTLKITRISPHQAITLRELNKKAIYFHKHFDWLEPEDIVSDGLC
                     YSLLENDIPIAGSGFSPEIDQTHWLQFFSVWEEGYSVAKVWKYFWENCIKLQIFSEKT
                     ILSIPADDLMNFFLQHSGFKQKGNIIYFEGEKEIISDHLLQNKQNATLLPLTESNVKE
                     IIDSVEVAFSKVWRLSELNLTRAIQASNIAFFLRDKNQSVGYLLALLEEDSIHISRIA
                     VSPNYQSRGYGKELMWILLNRFTPKNVNAFSVNTYSNDGNAVRFYQNLGFYRTNQIYP
                     VYYLKIPKN"
     gene            complement(26918..27634)
                     /locus_tag="PK898_00135"
     CDS             complement(26918..27634)
                     /locus_tag="PK898_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOU18470.1"
                     /translation="MIGTIINVAGILVGTFFGLFFGKRINEQIRNTIISGIGIFTLFL
                     GLSMFLKSEDSVVCLIGLVIGTIIGELTNIERGLENLGGWFQSFVDKNTRSDYGKDRQ
                     KFIQAFLTATMLFAIGPVSILGSIQDGLTGDYQLLLIKSILDGVTSIVFATTMGIGVA
                     FSVIPIFIYQGTITLLAEQAQSIMNDSMIAGMTAVGGVMLGAIAISSLLNIKKIRVSS
                     MIPALFITPFLFWITTHFNF"
     gene            complement(27715..29409)
                     /locus_tag="PK898_00140"
     CDS             complement(27715..29409)
                     /locus_tag="PK898_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU18471.1"
                     /translation="MKKIRWQLVIIFLTGLVVGILLLSDTSNGPIQMFESQPTMGGIY
                     REGLVGNIQRMNPLLSFYNNADQDVCRLVYSGLIKFDNRGLPVSDLAAGWAISMDGTS
                     YNFALNPNAKWHDGQPVTADDVVFTYEMLKKGNGYIPDDLIDFWNKIQIKAIDSQTVQ
                     FVLASAYAPFLDYLSVGILPKHIWGNMTFEQMVDSKVNIQPIGSGPFILDDLILNGSN
                     ITGIILKNNALYYGKKPYLEEIHINYYQDSILAFQAYQQGLVDGISYISEDVLESALT
                     EPDLALYTSRLPIQTMVFMNLDNGNVDFFQDKNVRKAMLMGLNRSKIVNNILHGQAII
                     AHGPILYGNWAYYDNIPKVEYDSYKAKELLKSSGYILANEQDQVRSKDGKTLNFTLLY
                     PDDDKHRQIAESIQQDWAAINILVNLEAVPYDELINVRLEERDYEAALIDLNLSDFPD
                     PDPYPFWDQTQVSTGQNYSQWNNRTISQYLETARVETDQAERERLYRNFQVVFAEELP
                     ALPLFNPVYNFAVDKQVKGVSIGPIYRTADRFQNIESWYLVSRTTLSDEFTGKNSE"
     gene            complement(29543..29764)
                     /gene="secG"
                     /locus_tag="PK898_00145"
     CDS             complement(29543..29764)
                     /gene="secG"
                     /locus_tag="PK898_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOU18472.1"
                     /translation="MKNFLDIALIITSIATVICVVLQNRGSGLGSIAGGEPGAVFSAR
                     RGIEKVLFYMTIGFSALLIIFVLVRILAF"
     gene            29861..30925
                     /locus_tag="PK898_00150"
     CDS             29861..30925
                     /locus_tag="PK898_00150"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOU18473.1"
                     /translation="MEEITVNLHNHSVISDGTGNYYEIAQAGLNAKIDVLIITDHNVV
                     VRGKDSYYYREGNKLLLLIGEEINDPIGVPGNHLIILNSSREYASAASDPQTVIDSVN
                     KEGGITILAHPFENAKKQLHMKSFPWSDWSVHDFSGLEIYNFMSRFKCGVTNFFSLPG
                     HYLRPINFQMGADENTLAKWDELLSMGYRVSGFAGSDSHRFQKKVGPKTFKIFDYELT
                     CRALNNHVYIPKPLVGDVEEDKKVIYAALRNGCFFVGADMITSTAGFSFRADTETGIY
                     YPGARIPIGNSATLKIRIPEKSICRLISNGKVYKQWESVQSVPITITEPGYYRVECYL
                     PYKNKLRGWIYSNPIYFFKG"
     gene            30934..31434
                     /locus_tag="PK898_00155"
     CDS             30934..31434
                     /locus_tag="PK898_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOU18474.1"
                     /translation="MTTTNNVIRFLSSKKIDFQVFESEPVRRSAMETAALYHLDPMIV
                     YKTIVAFNPIRKKYLLCIIPAPYEVNLKKLASAIGEKKAAIVTQHEAEVLTGLEAGGI
                     SPFALYHKGFQIWVHETIRDQEKISVSGGRRGLSVLLSTGDFIQILQPKIADIADIQQ
                     DIQSSE"
     gene            complement(32221..33261)
                     /locus_tag="PK898_00160"
     CDS             complement(32221..33261)
                     /locus_tag="PK898_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18475.1"
                     /translation="MLQLKDISYQYEKTPLLENLNLHLADHEILCLLGKSGSGKSTLL
                     RIIAGLEKPDHGDIFWNGVSILNVPPFKRNFGLMFQDYALFPHLTVFENIAFGLEMNK
                     VNSEKIPPIVEKLLKQVGMESFANRKVTELSGGEQQRVALARALAPQPHLLMLDEPLG
                     ALDHTLRVSLLAELRRILGENGIPSVYVTHDQDEAFSIADRIALLHDGVIVQTDTPEN
                     IFLKPKSVWVARFLGLNNLLPATGVTDSQILLKLNDRTIYLTIPLEYPLVEGQKIWIL
                     FRDEKLQLTNDADFSIKGIIKESIFKGSNYETHVEIAPGTVFSVINEKKFQAGTEIEL
                     FYSPEDLLALLI"
     gene            complement(33304..34965)
                     /locus_tag="PK898_00165"
     CDS             complement(33304..34965)
                     /locus_tag="PK898_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU18476.1"
                     /translation="MTAQRKTSSVTSIIKGSLLLLIPVGFFLLFYLLPLCTIFQTVIT
                     QFLEDFHINWDKIGKVFGFTTWQAVLSMILTMILGMPVAWIFSRYKFIGKKFLKSLFS
                     VPFILPTVVTAAAFNALIGPKGLLNVYLMQKHGFSQPPIQLINTIWIILIAHIFYNIS
                     VVMRIVGNAWENIDIKLENAASTLGCTPWQTFWKITFPLLKPAIFSAMLLVFLFDFTS
                     YGVVLLLGGAKFRTIEVEIAQQALQLFNLPVAGLLSILQIIVTVAVTSIENKIGKNIQ
                     SNRMPHVSEENMRKPTKPSEKIIIILILFMVAVFLVSPLLGLVIRSFVVYDSQSVAWT
                     TEYYKKLFVNERNSFFYVPPILAVGNSLLNATIASFISLMIGLMVTFAGDRYPWTKKI
                     NMIFLFPIGTSAVTLGLGYLLYFSHEINNPLLIPLAHSLIGLPFVIRSLQPVLQNIPI
                     SLHHAATVLGANPWKVFLKIDIPILRRAMINAGIFSFTISLGEFGATSFLSMPDRPTI
                     PIAIYRFLGQPGASNYGQAMAISTILMTFCLVGTFILESNSFQKQ"
     gene            35074..37506
                     /locus_tag="PK898_00170"
     CDS             35074..37506
                     /locus_tag="PK898_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18477.1"
                     /translation="MKKRNLFWEVFLFIGISVLLLGCDTKRNELIRGFFYTETPIPPT
                     PTVTITPTLSPTSIPTPLPEYRIDQADKYLLVGDYDNAISEYEKVLSSNPEEPYYAAS
                     VLGKARSYYFKRDYESCQNTLIDGLNTIKENSNKPDLWFQMADCVHELDLYEEEINAL
                     NEFLKLRPDTQMRSEIYERIGDSYYSLKSYKESQNAYLSAVEGTDMEESSWIWMKIAD
                     TYSVQEDYTNALKTWLDLYDLSTDDTQKARIDSLLADVYLKLDSPEQAYARYQDAVNN
                     FPRTYDSYLALLTLLNSDQTVNEYQRGLINYYQGQYALSSEAFHRYIESNPAHNGSAY
                     YYIGLNQMFLGDYQSAVETFATLIENYPDNQFYASAWDEKSYVEWYYLENFAQGAQTL
                     IDFVKLHPDYPEAPGFIYEAGRIFERGDRLTQAATQWERLIDEYPLYENSIDALFLSG
                     ICRYRTKNYDAALSTFNRLLLVSTQPADIARAHFWIAKVYDKKGESGSAKQNYESAAQ
                     DSPTDYYTERAKEILAGKKPLSFSENYNLTTNLSQAQVIAEQWMRLTFSIPDEVPLDQ
                     PDALLLDSDYRHADELWELGHYQDAIAMFDAVRLKYENDPVNSFRLLNRLVSLGAWRP
                     AVYTSRQILTCAGLIEDARTLEAPNYFNWIRYGTWFNEIVLKVFDQYKIHPFILYGLM
                     RQESMYDPWISSSAGASGLMQIMPATGLELSKKLQWPPNYADSDLLRATVAINFSADY
                     LSTQHDYFGNDNFYMLASYNAGPGSTSGWVDKAENDPDLFLEIIRFEETRTYIRNVYE
                     YAKMYERFYGVD"
     gene            complement(37511..38917)
                     /locus_tag="PK898_00175"
     CDS             complement(37511..38917)
                     /locus_tag="PK898_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18478.1"
                     /translation="MAKQYDYSEIFESIKKVVPSSVQIYLVGGAVRDIILGKKVRDFD
                     FVIEGLVRPVGRNLANELNGKFYVLDDDRNMVRVLLNADSDEYYCIDISQLNGSTLEE
                     DLYSRDFTINAIAIDFIQKNKFIDPMGGVSDLKEKKLRMCNLESIKSDPLRGMRAIRM
                     AVDYDLTMDGDLIHALNEIRPFLSVASVERYRDELFKILDGEKTVTAVRLLDKYAFLD
                     FLFPGKNPTNDEALYDWLRSAEHLINILAREFREDESSNLISGMAVLKLGSFRDQLNE
                     FFYHDNNLIHERRSLLIFSIIARFYRTEENSRSVSEILKSRSKKMLLSTAEMKLIIKS
                     YEAFMELHSLFNLEEYSDVVIYRYFQKFGDYGIDGIFLKLIETHINKDILRDSAQWTK
                     VLDHAARYFDAWFNHFDTIIKPKPLLSGDQISKLFNIPAGPEIGELKNAILEAQIENL
                     ITDREGAIKFTRNLIESS"
     gene            complement(38921..39487)
                     /gene="hpt"
                     /locus_tag="PK898_00180"
     CDS             complement(38921..39487)
                     /gene="hpt"
                     /locus_tag="PK898_00180"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOU18479.1"
                     /translation="MYSYQDVLAEVLIDETTLQKRVKELGEEISRDYQNEDIVFICIL
                     RGGVVFLIDLMRHVTVPNQIEFMAVSSYGVGTRKSNGEVRITYDLNTNIEGRNVIIVE
                     DIVDSGNTLNAVIQVLSTRNPKSLCICTLLDKEDRREVEIPIRYCGFVIPDKFVYGYG
                     LDLDDYFRNLPFIGVVDLEKLEKIKGKK"
     gene            complement(39503..40078)
                     /locus_tag="PK898_00185"
     CDS             complement(39503..40078)
                     /locus_tag="PK898_00185"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU18480.1"
                     /translation="MVKKAPAAIEVICGSMFCGKTEELIRRLRRAIIAKNKVQVFKPI
                     IDDRYIIERVHSHAGIEIDAIPLAEITDLYKYLDPETSVVGIDEAQFFGKEIVNIVDD
                     LANQGIRVILAGLDMDFRGEPFGMMPVLMAKAEKVDKLYAICMVCGEPATRTQRIING
                     EPANYNDPVIIVGASEMYEARCKKHHIVPGK"
     gene            complement(40234..41796)
                     /locus_tag="PK898_00190"
     CDS             complement(40234..41796)
                     /locus_tag="PK898_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOU18481.1"
                     /translation="MRLVSVSEMRQIENQAFGAGISSDHMIQTVGLKIANILDSDCSD
                     KNKMVLGLIGKGNNGSDTLAILTYLAQKGWETFAWLLSKRESEDRYLKDFLNVSGKAV
                     DYENDTEESQLDNWLKQSSIILDGIYGIGFHLPLGEAFRPIFRKINQRKNPSLIYAID
                     CPSGMDCDTGKIDPDCIRANRTICIEAVKIGEVLPPANLLVGELETISLDLPQDLAVY
                     QTIHRTVLDSEVVRMFLPIRPEDGNKSTFGTVTIVGGSEKYIGAPKLSGIAAFKAGCG
                     YVNMVAPEIIRNLNAGIFSQAIWQPADFNSDEGIEIALHSIRDMSKKKGSLVIGPGLG
                     QSPKVFDLVHRILISWKENPVPLLMDADGLNALPHIPDWQKNIPDCFVITPHEGEMAR
                     LCNMTVDEIKTNRIQIAEQKSKEWKTVIVLKGAFTVIASPDGYTAVMPYANSILAKAG
                     SGDILSGLIGGFLAQRSLPFEASCLGVWVHAKAAQIAFRQIGQPYSMSADDLLSLIPE
                     AIRMIYQSLPKI"
     gene            41891..43438
                     /locus_tag="PK898_00195"
     CDS             41891..43438
                     /locus_tag="PK898_00195"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOU18482.1"
                     /translation="MKRFIFFSVFIAGFSSMAIEMTASRMIGNIFSSANLIWACVIGS
                     IMIYMAIGNWLGGIFSDKYPDLFHYLILIFISGIFCCLIPLFSRPLLLKGAQAMDQMT
                     LRWVLILFLILLLILFIPMTLLGMIIPFAIKLLNTYYPFRGKVNGQLLAVSTVGSFIG
                     TMLPSFILIPSVGTNRTFFVIGFLLLLAGAIGQLTNIGSDKKAEKLMIFCIFLPILLF
                     FLFPLKIKQTKGQIYEKESVYNYIEVVENSGYTILRLNDGQGIQSIYHPVIDNYYGPW
                     EQILTAPFFQPAPIDPGNIRRAAILGNAGGTSAKQLLKVFPNVIVDGFEIDPEITKVA
                     KEFFDLPSERMNIISTDGRIGLQNSDDQYDIIMVDAYQSPYISVNMCTQEFFQIVFEK
                     LNPEGVLVMNIGRSDDDRTLLGAISETVNRIFPQIFIVDIPNSYNSVLFAMKKGGSIQ
                     NLQNNYDLLSGLKNVPALLTETLSFAIGNIGELPEKRGLVFTDDKAPVEAITNQTILR
                     TFFKRNE"
     gene            43460..44161
                     /locus_tag="PK898_00200"
     CDS             43460..44161
                     /locus_tag="PK898_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HOU18483.1"
                     /translation="MKIIIKSFHVFLSITLPFVILMFSVVILFKPWFLVFEYRKTNFP
                     PDPYGFSIEERLDYGIQSVQYIVNNRSDDFLTKITFKNSNTPIYKSQEISHMSDVKNV
                     YRTARLIWEALLILYLILFLVSWKHPAQLLSFLKHFIIGNLFSILFLVAVLLLVVLAF
                     DPLFEAFHSLFFKEGSWLFYQDDTLIRLFPEKLWVDAFLIAGGLSFIFSVAFFFIGRN
                     AYRRISINSRNGRPL"
     gene            complement(44225..44413)
                     /locus_tag="PK898_00205"
     CDS             complement(44225..44413)
                     /locus_tag="PK898_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOU18484.1"
                     /translation="MKAKVDQSVCIGCGLCSDTCPEVFHMTAEGVAEAIEEEIPSEFE
                     SSAKEAADNCPVSAISLE"
     gene            complement(44415..44768)
                     /locus_tag="PK898_00210"
     CDS             complement(44415..44768)
                     /locus_tag="PK898_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU18485.1"
                     /translation="MNQFIKNLEFSQVFKFADQVSYLPGQIVSKTLAQNESVSLTLFA
                     FDEGEEISTHESSGDALVLVLDGQGQITIDGEKFDLKTGDSILMPAGHPHAVYGLKRF
                     KMFLIVVFPPKNKGE"
     gene            complement(44779..45486)
                     /locus_tag="PK898_00215"
     CDS             complement(44779..45486)
                     /locus_tag="PK898_00215"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU18486.1"
                     /translation="MIRKMVEIDEEKCTGCGLCAEACRENAIQMVNGKAKLIRDDYCD
                     GLGNCLPVCPANAITIIEREAIPFDKEAVRHQATVNNMTNVPSGCPGKQAKTLRRDTS
                     NPIMESSRESQTSELMQWPVQLKLVPVNAPFFDNCNLLIAADCTAFAYNNFHRDFIKK
                     HVTLIGCPKLDQEDYSKKLTDILRNNSIKSVKIVRMEVPCCKGIENAAIQALSSCGKM
                     IPWQVIVISTDGNIIEE"
     gene            45578..46276
                     /locus_tag="PK898_00220"
     CDS             45578..46276
                     /locus_tag="PK898_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU18487.1"
                     /translation="MKSYDPLLLQMPLFYNFSPKDLDSLRNCLSFRVRKYLKGEAIFL
                     EGDEISRLGIILNGSVIIVKEDISGNRNIISTFSTGNLFGEAFAFSKQKRIMVSVFSE
                     SDSEILFLDAEHLLTLCKELCPFHSQLIRNLMNEFAEKTILLNEKIDLLSQKTIQDKV
                     LAFLFSEAKRQGSRSVQIPFNRQQLADYLFVDRSALSAELSRMQKKGILEYHKNKFVL
                     KTIAHLDDNPSFNL"
     gene            46328..46918
                     /locus_tag="PK898_00225"
     CDS             46328..46918
                     /locus_tag="PK898_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18488.1"
                     /translation="MEQKLSFCTSEILIRNMRSPLPYGLTVIQGPHAASGLLLQICAK
                     LALRGPVRILDCGNRSDMYQVARELRPLTRDPATAMQRIHLSRAFTCYQAEALLGQNR
                     GQSQTPILIFDLLATYLDESVSKQEAERLFDDTLDHLQKMSRKNPVLVGIRPLPAIAL
                     DRSNLPLRLQKAADESFQLMEISQKAETNDDQLSLF"
     gene            46940..47287
                     /locus_tag="PK898_00230"
     CDS             46940..47287
                     /locus_tag="PK898_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18489.1"
                     /translation="MGRTIRSATQTWIEEEKALGRFARALRKEDQRAMMELINLSRLH
                     IAESSYASNLYPMDVYLISMLLEMFKRVQRDEATLAEVCKHAGIELPSVSTEIPEFPD
                     LVDLIESLDQADA"
     gene            47299..49515
                     /locus_tag="PK898_00235"
     CDS             47299..49515
                     /locus_tag="PK898_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HOU18490.1"
                     /translation="MQMHARGWLLDLYEDANDGVRLWFILENDQRICLKQQLPITFYI
                     SGDSKRLHQCCLFLKHLPGMIAMQRVSKKDVFKTEPVCALQVDMADPVHQRQAFLKLK
                     KDFPDLTYYNADLAVHIHHAALYQTFPMAFCDLLYDSESGLIQMLKVLNSRWDITPLH
                     PVLRILEIAPDCDPIRKKPEMLKICDRRGHKQTICLREPTLLLEHLNALIAATDPDIV
                     ETQWGDDWLFPLLLQWEQETGIPLNFNRDPERKLRWKKEITYFSYGQIVYRGREAHLF
                     GRCHIDVRNAIMWKDYGMAGTLESARVTTLPIEQASRVSPGSGISAMQMITALEEDVL
                     VPEQKQQVEFTKTGLDLIRSDRGGLVYQPRTGLYCDVAQIDFTSMYPAVIINGNISPE
                     VPLPQGLKPSSPNLGVVPLTLKPLYEKRVKIKQRLLEMPDKEGTVAKSYTARASALKW
                     LLVVCFGFLGYKNARFGRIEAHEAVTRGGREALLLAKEACEDFGFEVLHLFVDALWIR
                     QPGYRAVADFQPVLDEISRRTGMIISLDGIFRWIAFLPSRTNDNQPVPNRYFGVFKDG
                     SLKIRGIEARRRDLPRWVIDTQTDMLNLLAKATDARQLPDYLPDAFKIFTHALDELNS
                     DRVPLESLVLTNRISRDLELYKSPTAAVRAALQLFEQTGKRTDPGQKIRYLYTKNDVY
                     AWDRPGSLSPDQIDKSKYREMLARAAGTVFYPFGIDSKQLSAYAHKSLQFELSLMK"
     gene            complement(49577..50689)
                     /gene="ugpC"
                     /locus_tag="PK898_00240"
     CDS             complement(49577..50689)
                     /gene="ugpC"
                     /locus_tag="PK898_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOU18491.1"
                     /translation="MASVTYDHVWKKFGDVEVLKDIQLAINDKEFLVLVGPSGCGKST
                     ALRCLAGLEEVTSGEILIGDRVVNDISPKDRDIAMVFQSYALYPHMTVFDNMAFGLKL
                     RKTPKEEINRRVNEAAATLGLERLLQRKPRELSGGQRQRVALGRAIVRNPQVFLLDEP
                     LSNLDAKLRVQTRSEISKLHQRLQTTFIYVTHDQVEAMTMADRIAVMYKGIIQQLDTP
                     QNLYNRPDNLFVAGFIGSPAMNFFNAKIKVEEGNVYVVTDSFTVQIPDDRKDTYAPYD
                     GKEIIFGIRPENVHNPEFCPPGIKTAEIEAVVDVIELMGNEIFLYCMTGDTQFVARVD
                     PRSHYSIHDKVKLVFNMEAFHIFDSSEDKENPKAVR"
     gene            complement(50812..50990)
                     /gene="ssrS"
                     /locus_tag="PK898_00245"
     ncRNA           complement(50812..50990)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK898_00245"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(51037..53694)
                     /locus_tag="PK898_00250"
     CDS             complement(51037..53694)
                     /locus_tag="PK898_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18492.1"
                     /translation="MEKAKINRTQLIDLSNAAFQLGNFDCARKLLIAWLKEFPNDLWI
                     RYRLAIVLYKSGKTNEAIRLSEIILKYDPEFFEVWALLSVLYPENSEDKKIALSRMKL
                     IKSYQKGKEGSRNKNIFGFFNRGLTEKNLECIDDVDDFDIITAIHFSLNSDQQKDLES
                     YFRLMKIYTRRWPNAIQFKLLLGDVMNKMGNVEEGTKLIHSTLENDLLGQVANRTWKE
                     NNVYKDLWIDSAALSIDVSKIQIPLKVGKKARLDHTINISWSDSDSKLSNRSDEFITA
                     ADAKDVFMEAPEKNDESLPNPIAIADESMIKQEESELSDREKISPQKEMKTLITSNGN
                     LSSLIGFLKKIFTGRWEKQTETIDSIKEFIYKLDPDDADERFPVYVILSTVAGLTAKY
                     GKNNKDFIDQEMRSAADAIENRNGWNAMVFYPDEFQANSKTVLDPQSIRNSIIKLDES
                     LAQKGSMIGALLIVGGHDVVPFFKLSNPAMDDDLSICSDAPYGSPDSSRYYDQQWQVG
                     RIPGDSSDDPGLLLSQLRSIQNYHIAKYEQEQSRSKRKSAKAPRGAKIKKGVKSFGYC
                     CSVWQRPSVAIYRNLTDSSEMLVSPPTMATNFPSSRLEGIDFGYFNLHGIKGQPNWYG
                     QKDSKDTSTIPMIPVALQIQNLQPVTKTPKVVFAENCYGAEILNRNESNAISLHMIGK
                     GTRVFIGSTVIAYGAMSLPLTAADLLAHLFWKHLMTGISCGEAFRRAKKNLATETESN
                     SGALDGEIQKTLISFIFLGDPLYAVDDNADITDRMQRAKTPRSYELIHERLDSKVAID
                     TDLAKKIYEEVKEMYKMEGMNDEFSSFTIQKQILSAKSQDDRQRYIESPNYVVVFTKD
                     AKLGNMTDRLITRVTVSKDGKIKKVSFSR"
     gene            complement(53719..54027)
                     /locus_tag="PK898_00255"
     CDS             complement(53719..54027)
                     /locus_tag="PK898_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18493.1"
                     /translation="MINTSITFSIAGIVLFIVSCIAIIMGIVIIISSVFSKNAQEIQK
                     QAAKLSSKGFTDEMSGALGNASTLIRELNTLIETKRGVGLSLVLIGFILAALCYLFLL
                     "
     gene            complement(54046..55221)
                     /locus_tag="PK898_00260"
     CDS             complement(54046..55221)
                     /locus_tag="PK898_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU18494.1"
                     /translation="MDFTVLVVDDEENFREGIKQYLSRDVNYEILDAASLSEAREILN
                     RQPVDIIMLDVQVGKEYGPDLLYDINRIRPTPKTILITAYGEVEMAVDAMKNGAFDFL
                     SKPVSFPLLEATLKRAEDVIRLQKEIESLMGAYKKDFSFVVGQNEKMKRIFKDAQLAA
                     KAQVSILIGGETGTGKEVLAKFIHQNGPRANKLLVPINCAAIQPTILESELFGYEAGA
                     FTNAEKRKPGLFEVADGGILFLDEISSMSMDMQSKLLRAIEEKKVRHVGGTKEIPVDV
                     QIVAASNRNIPEMIEKGDFREDLYYRLRIVDIEIPPLRERKEDLPELIGFFVKSISAE
                     RGVNITKVSEPVLRAFQNYSWPGNIRELKNAIERASIFCEGDTINLCDIPADIAKFE"
     gene            complement(55226..57136)
                     /locus_tag="PK898_00265"
     CDS             complement(55226..57136)
                     /locus_tag="PK898_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU18495.1"
                     /translation="MENISIENSFEVKTICDSFLYSCITPAFIWAVQEGRLISANPMG
                     VEFLNSFRSSVDNAELSDLIRNWKEIPDEDHHTMWFEFRSQDGRIKPVQVRNSYLTPE
                     KTILASVVINPRWNIETTGSQSMDALLHIIGNASVTFCSRTDFLEGFESFMDMTSVEL
                     GLSEFGFIPEPIFEQYDLYDRLQKNIMSKSIYPYIEENLSHFSSFEGDRLSDVSDEFA
                     GSPIHFKIFPVSNSEQRIGYFILRFQDSALYRLDFYQTVLEILSIYFQGLFENMEMTK
                     SLYQCEFQNSLNHQIIDNISEGIIITNNEYQIVYINQISSIMFGFAPSDVVGHPLDDL
                     LVTNEGVRKIVQTLSSSENNSDETPFIQYLHRRSGESFPCHLRISTLDFDEKNSYTIF
                     VLTDVSESEESRLKTEQLTQRAFLGDFASLLAHEIRNPINNMNIWIQNIKSLTNEQSD
                     IYQAVNRIEEDCSRVSVLVNNILAFSKPLKLNLEEIDLEQYINEIIERWKLNFARANI
                     KTFFSAPENLPKVLIDPRSMEQVFNNLIGNAIDAFDNKGGVISFKISLHDSKTGRKQV
                     QISVSDNGPGIPDEIIDHIFEPFVTSKKKGNGWGLAVSKRIITSHKGTIQVKSYPGGT
                     VFEITLPISTGV"
     gene            complement(57142..58551)
                     /locus_tag="PK898_00270"
     CDS             complement(57142..58551)
                     /locus_tag="PK898_00270"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18496.1"
                     /translation="MYQRQKQELRQQTSAHLAQTMTILSMSADEIREKIASELSVNPA
                     LELTDERICPTCHRLLPDNGICPNCCTLKSEDEESIVFVSPRDDFAVENYAVHSELSH
                     EPDMDSAQHEDLPTYVLRQIGPDLEDDEKMIAAYLLTSLDEDGFITSSVLEIAQYYHV
                     LPSKIEEVIQLIQHADPLGVGSRNPQEALMVQIQHLSGLIDIPDKTLDVIRDSYDLLI
                     HGKYREISKIHDLTTKQINTIEKFIGENLNPYPGRAYWGEKGIDNNVDNNTYHFPDIL
                     IYYLNDNPKNPLVVEIIMPISGNLKVNEFFKRESKNAPEDKVEEWKNDIDRASLLIKC
                     LHQRNNTMVRLMEVLVDLQGNFIRYGDSNIVPITRAQISKLLDVHESTISRAVANKTV
                     MLPNKKVVPLSAFFDRSLNVRTIIKDLVKEEKKPLTDAKIVEVLTGMGIDIARRTVAK
                     YRAMEGILPAHMRNSANLR"
     gene            58773..60131
                     /gene="trmFO"
                     /locus_tag="PK898_00275"
     CDS             58773..60131
                     /gene="trmFO"
                     /locus_tag="PK898_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOU18497.1"
                     /translation="MVKMFDMGSNNQSDLCIIGGGLAGCEAAWTAAQRGVHVILYEMR
                     PQKMTEAHVTGNLAELVCSNSLGSNLIDRASGLLKEELRFLGSLLIDIAEKTKLPAGN
                     ALAVDREAFSIAIENAVLSHPNIQVIRDEVKYIPDDLPVIIASGPLTSESLCKDIVRL
                     TGEENLYFYDAIAPVVYADSIDFKIAFRASRYDFDNSEEGDYINCPFSKEEYDRFVDL
                     LTHAERIELKDFEKDILSGQQKFFEGCLPIEILAARGKDALAFGPLRPTGLKDPHTGH
                     RPRAVLQLRQDNLAGDLYNLVGFQTNLRYSEQERVFRNIPGLENAVFARFGQMHRNTF
                     LSSPGLINEHLQLISDPRIFFAGQLAGVEGYMGNIGTGLVAGLNAAAFIHGDPLPLFP
                     IETMLGSMIHYLANSEKKNFQPIKANFGILPPLENPPRSKQERGRQYAIRSLDTLKNL
                     RS"
     gene            complement(60142..60369)
                     /locus_tag="PK898_00280"
     CDS             complement(60142..60369)
                     /locus_tag="PK898_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOU18498.1"
                     /translation="MSEKLTGTVKWFNTSKGYGFIAVPGQKDIFVHYLDIRSDGFKNL
                     SEGQQVEFIPVESSKGLRATEVHVIDKEKKE"
     gene            complement(60422..60682)
                     /locus_tag="PK898_00285"
     CDS             complement(60422..60682)
                     /locus_tag="PK898_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18499.1"
                     /translation="MILPAIIFSFIMATLYACIFHLIRGGNLFTLFIYIVVSNIGFFG
                     GQYIAELMGIKFLTLGTINFGVGTIVSIGLLLIGGWISRPIE"
     gene            complement(60682..61776)
                     /gene="mnmA"
                     /locus_tag="PK898_00290"
     CDS             complement(60682..61776)
                     /gene="mnmA"
                     /locus_tag="PK898_00290"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOU18500.1"
                     /translation="MIKKKQRVIVGMSGGVDSSVAALLLKQQGFDVIGVMLKLWAEEG
                     FDRENQCCSIEAANQAHRIATKIDIPFYLLDAVEPFYESVVQPFISSYFLGETPNPCA
                     WCNPLVRWRQLLQYADAMDAEYVATGHYVRTIKDERGITHLFRGTDPNKDQSYVISRL
                     SQRQLQRSVFPVGDYFKSQVRKISAEYHLPYSEKPESQDICFIGDGDYRRFLKHMQPQ
                     RISPGKFIDSEGKEIGQHDGIPFYTIGQRKGLKISAKNPLYVIAKDVIRNEIVLGQKE
                     DLRVKKVVADDINWICNEQTVDSFDCFAQFRYKSKAVPVSVRITEEKKMIISLKDDFF
                     DGVACGQIAVLYDTEGEVIGSGRILKTERK"
     gene            complement(61779..62951)
                     /locus_tag="PK898_00295"
     CDS             complement(61779..62951)
                     /locus_tag="PK898_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOU18501.1"
                     /translation="MKRLNIENYFDYAATTPLLPTVFTRMQPYFTNLFGNPSSAHRFG
                     QQAYGAVEDARNILLDHFHITNPDNLIFTGSSTEACNTILWATVFQYWKIYGKPGRLL
                     ISPLEHSAVNETARFLQDAGFCIIDQLPVNQYGQVDPDDVRKMIRSDTVLVSVIYGNN
                     EIGTINPIPEIARVCKEKGIPFHTDATQYVAHNQIDFGNSEISMMNIAAHKCYGPKGT
                     GAILLGNGVENFLPFIHGGKQENGKRAGTHNVPYIVGMAAAYQILDNEFDKRIAFERQ
                     KRDLLIQLISENIPDCLLTGHPTERLTNHASFAFKGIDSLVLQSLLDQKGFAVSIGSA
                     CRSSKIHGQQQLIDIGLSSEWANGGLRITVGIYTTDDAIYSLVNALKRSIRILRRS"
     gene            63050..63619
                     /locus_tag="PK898_00300"
     CDS             63050..63619
                     /locus_tag="PK898_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18502.1"
                     /translation="MDQIFQFFNQIISWFSGLSLAEKLGVIGFILSSLISIIYIIKWI
                     AHLFRREKRKVSLSFKLEDVTHEMFKKKGFVTFDLYVRNDGDSPIEILQSGFILADGT
                     EIPIYEEPHKIKAPDNVIVQPGHEAFYDNCDLFQILNVNGVNYKKIVGFYADVKGAPR
                     FRTEGDIHPLMNDQIVRGILTDKKGLNGF"
     gene            63672..63743
                     /locus_tag="PK898_00305"
     tRNA            63672..63743
                     /locus_tag="PK898_00305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63704..63706,aa:Gly,seq:ccc)
     gene            complement(63785..66352)
                     /locus_tag="PK898_00310"
     CDS             complement(63785..66352)
                     /locus_tag="PK898_00310"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU18503.1"
                     /translation="MVSNLPIKNHFNKILACCFLLPFLIFIAILAFEKVYPFGSNSLL
                     YSDLSDQFIGFLSELARIYKGEGNLFYTFHAAGGINFFPTFVHYCSSPLYLLLIFFPE
                     ERMIDAVTVIILLEISLSGLTFGYYLGKRFNCLNKPIIVPFAVCYALSGYSIAFYFLF
                     SFQNAIILLPLVCWQIEKIISRDQWGGCVILYFLLIVSNYYCGYMASLFSCFYFFIRI
                     ISTDLSISRKEKIRKTGLILISIILATGLSAFLWLPLFRALKENADLIGQPFPIHHFN
                     FSAFELFPKLFIGIGDGVKANLPYIYCGILPLISGCLFFLNKQIPKKTKIFSGALYII
                     LILSFTDQILNFIWHGFDYPNWFHYRFSFVFSFLLLTCSFEFLIKLHINNLNIILCGV
                     FLILGLLIFHQIYPDRIKSYQIFINLFFIVVYSLFLVVLNRGKRNRHIPYFLWIFCSI
                     ECFLNGILLYKENSVVLTDRNSYLDYHRKYADLIDRYSSKIPGDFYRIEKTDRRSMSN
                     DSIELGYNGLSLYTSILNKKFQALTQSLGITSINYWIRYEGSTIFTDSLFGIKYLFSD
                     YALNHDYDEIESFVWSNPYPFPMFFYSDSALNSVGSSAYENPVLFQEAILEGIAGETG
                     RFYESVPIQDISLDNLVYSDTEKDQVRVLNPAQPSSITIRFKTSRISPYYLFIPQVIG
                     GYELLINGTSVDIDPYMSNGIQYIFNLYPYLKERESSVIQINFHRQDWQEFQLPSVYE
                     FDIDRFSILTKKIQEDAPQVIQTGFNSFKILLNQEANNLILVSSIPFESGWQVFLDGK
                     RIRYDDILSGLLCIKNIPESAHEIQLKFIPPGFRIGLVISILSIISVLMFGINRYKK"
     gene            66495..67421
                     /locus_tag="PK898_00315"
     CDS             66495..67421
                     /locus_tag="PK898_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU18504.1"
                     /translation="MAYLKIISSTFSLFVAIAVGFFAGKKDFLTESSTTELSRFVIRI
                     LFPIYLFRSAVTYINKENFLSAPLYTFLNVIVMIAMWFISRQIVKKANISPDRIPMFE
                     FTSLAGNTGFFGLTFATLLFGSEEIMAAILYDFGGSLVIFMILVPFLLDNNHIHHPIN
                     FLKEPNVIAILSGLILGILGVKIPEFLAPSFNLLSQITLPLALILCGAQLASIKISKE
                     FHFSQVLYVILIKMILMPLLLFVIVLWIPLDGQMKQFLVVDAALPSGISMVNFAKTYH
                     QDSDFAAVAVFATTAFSVIWLPFLLIVLSSIF"
     gene            67605..68648
                     /locus_tag="PK898_00320"
     CDS             67605..68648
                     /locus_tag="PK898_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU18505.1"
                     /translation="MKITLKQIAREAGVSQATVSRLVNQNTGVSSELEEKVTNAAKKL
                     GVELPRKSSAVKSDQTKNKVIGLLVTDLSNPYFQVIMRGVIDEARILNYGVQIFETYE
                     DIRSEEEAIKTIERFKLDGAILSASRISENKLINFHKNTKIPIALMNRYVKEPEICCI
                     IVDFQRSLFQATTHLIDLGHKKIAYLSGPSNSDPSKTRRAGIVKALEQANLKLEADLC
                     VGSFPNVEGGFQAMISLLSLSPDKRPTAVLAYNDLIAVGALRAIRMKGLQVPNDISII
                     GVDNIDMSEHTYPPLTTISPPKVKMGSIAMRAIYRMNMGEYYPTDGYIEVDAPLTLRY
                     STGPVKHNPNESI"
     gene            68676..69131
                     /locus_tag="PK898_00325"
     CDS             68676..69131
                     /locus_tag="PK898_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU18506.1"
                     /translation="MAKEQYVFESEGKIIRYRFPTHINDLIIPREESTCAEVFMVVLD
                     ENEAPPLHKHDDAEQIFYMIQGKGKLEIRKQQNDEPEYFEINVGQVIKIPPHIWHRVF
                     ALSKEGVRYLSIDCFPNGFDPSEPTWDDHVKNMATANGWDFSKIREDRR"
     gene            69216..71144
                     /locus_tag="PK898_00330"
     CDS             69216..71144
                     /locus_tag="PK898_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU18507.1"
                     /translation="MSRRISILGIILVFATMFLIAGAVSAADYHEAPMLADKVAAGEL
                     PPVAERLPEEPLVEQPLESIGVYGGTLRRGITGPNDHNSYVRWVYDSLVRFSQDGNST
                     YPHLIKSLEPNEDYTVWTFNMLKGAKWSDGSPFTSADIMFWFHDYYTNPDLAPAKLPW
                     LYNDDGSLVEVTAPDDYTVVFTFKDPITTVPMEVAAMDNRDNLVPMFLPSAYLKQFHA
                     KYTDVDKLNQMAKEQSFENWKQLFENKQAPFRNPERPVMAAWMSVTTINDPIYTLVRN
                     PYYPIVDTEGNQLPYIDQINFVTYQSTDALNMAVIQGELDMQDRHVVMQNYPALVEQA
                     EKTGKYEVRLWPTFGGVDAALSVNYYYDKDPDYMKLFQNHDFRVALSLGIDREEIIQS
                     AFLGVGEARQPVPRNTSPYYPGDEYAYKYTEFDQAKANEILDGLGMTKRDSQGFRLMP
                     DGSRCMIEIAYVPQFANWGDIAQMISEDWRGIGIDGVVVENERTNHFAKRDSNELMTE
                     IWNEDTTGFPFTGAPKFDPRTSPGICVATGARTWLNSGGKEGYEPNADFKRIMEIIDS
                     AKRVSPDKQAELGKELFRIWAEGCYEIGIAGLTPLVQGVAVINVNLKNVPASNELGND
                     WPLRTPGNARTEQFYFAK"
     gene            71298..72281
                     /locus_tag="PK898_00335"
     CDS             71298..72281
                     /locus_tag="PK898_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18508.1"
                     /translation="MGKFLAKRLLLIPFLLLIFSIIIFALIQAPPGDFMTTYSAILAG
                     SGSSLDAEQLNAMRVSYGLDQPMHIQYIRWLGKLLHGDMGLSLLYMLPNKQLIGERLG
                     PTILLALFSFILSWLIAIPSGIYSATHKNSFGDYFFMVINYIGVAIPNFFLALLLMWF
                     FYSHYGLNLVGLFSPEFEMAPWSYAKFLDMLKHIWLPAVILALGGSARLTRVMRANLL
                     DELGKPYMITGRAKGMSEWALIMKYPVRLAINPLVSTIGNYIPQLFSGSLIVATVMNL
                     QNIGPLLLQALSMQDMYLSGSILIIYCLLGILGTILSDLLLAWVDPRIRLE"
     gene            72287..73483
                     /locus_tag="PK898_00340"
     CDS             72287..73483
                     /locus_tag="PK898_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18509.1"
                     /translation="MTENYSPKNNSNPTKNNPSESSVENQANIDLDALASTKSEEKVS
                     IASNGQLIWWRFRKNKMAVISLFILVIVFAIILFPDFFANQNSEKTNAREAYIPVQSL
                     HFFKDGKPDLWVDKVEGQRNPKTLGMEWVVKEGESVDVKFFGKGMKWKFLFWETERHL
                     ITPSDPNTRVFLLGSDRMGRDQFSRLMYATQTSLTVGLVSVLLSVFLGVLLGGISGYF
                     GGMTDMVIQRLIEILNSIPYVPIWMAMTAALPRQWSAEQRFFAITVILSLLGWTTLGR
                     EVRGKFMSIREEDFILSAELIGCSKWRIIMKHMVPTFLSHIIATGTLSIPAMIVNETS
                     LSFLGLGLRPPAISYGVMLQEAQNVQTMAKAPWLMLPGLVLVINVLMFNLIGDGLRDA
                     ADPYSK"
     gene            73492..74526
                     /locus_tag="PK898_00345"
     CDS             73492..74526
                     /locus_tag="PK898_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18510.1"
                     /translation="MENTKPLLSVRDLKVYFDLDEGTVKSVDGISFDAYEGKVVGIVG
                     ESGCGKSVTMKSILRIVEKPGRIVEGDILLQKRDNPDEYLNLVKMKENGKEIRAVRGG
                     EIALIPQEPMSAFSPIHTIGDQITEAIMLHNPVDKEEARRICIESLKSVGMSMPEKRI
                     DSYSWQLSGGLRQRAIIAMAIVCKPRLLIADEPTTAIDVTTQAQVLKLLKKLQMERNM
                     GILFITHDLGVIAQMAQYVVVMYLGRIMEKGPVDSIFHNPKHPYTQALLRSIPSIQKT
                     TKVKLPTISGSIPHPFNKPSGCPFHPRCNNWIRGKCEKLLPPEYIVGEGQSASCFLYE
                     GSEEVTHERD"
     gene            74513..75565
                     /locus_tag="PK898_00350"
     CDS             74513..75565
                     /locus_tag="PK898_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU18511.1"
                     /translation="MNETNNPSIQMTNTERILEVKHLKKFFPITKGFRKKHIGDVRAV
                     DDLNLYINKGETLGLVGESGCGKTTASRCILRAYEPTAGDILYRTKTGVTVNLSQMSN
                     KELRPLRAEMQMIFQDPYGSLNPRMNIFEIVSEPMLVNGIKNRREREDRVAELLTKVG
                     LRPEYMVRYPHSFSGGQRQRIGIARALALQPNLVVADEPVSALDVSVQAQVLNLLMEL
                     LEEMNLTYLFVAHDLSVVRHICDRVTVMYVGKVVETASTEALYNTPRHPYTEALMASI
                     PIPDPRLQQNREGLPGEVPNPSNPPSGCYFHPRCSYCIDKCKTEMPELKEIEPQHWTA
                     CHRAEELKLEGFHKNE"
     gene            75558..76739
                     /gene="nagA"
                     /locus_tag="PK898_00355"
     CDS             75558..76739
                     /gene="nagA"
                     /locus_tag="PK898_00355"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOU18512.1"
                     /translation="MNDFQAVAIQNSKIIFPEKVEEGKVLLLRDGKIAGIVGRDDFNP
                     ADYLTIDAAGKYTAPGLIDLHVHGCYNHTFNESDPEAFNTILRNTLRYGITTLVPTLV
                     AAPIPNLIQSLCFIDKWKSEQKPGITQITGAYLESPYIAPQAAGAIPASALRKITDGS
                     IDQILELSYAISIFMIAPELEGATDAIRKIKQKGIIAAMGHSMAMESEIIPAIEAGAS
                     HVTHLWSAMSSVVRQGPWRKPGLLEVALVYPQLTTEIIADNRHLPPTLMKLAVKAKGD
                     KVCAVSDALNGAGLPEGSHFFVGENEYEVVDGVGMVADRSCFAGSTTLLGQEIPILVK
                     EVGLSIPEAIRMVTEIPAKILGIADKKGSIRTGLDADIILLDSNFKIHEIFQQGILIQ
                     F"
     gene            76770..77546
                     /locus_tag="PK898_00360"
     CDS             76770..77546
                     /locus_tag="PK898_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU18513.1"
                     /translation="MEPIFTDQWGKLPVKVYETNEMMGEAAALDAREIINSAIAEKGF
                     ANMIIATGNSQLTFLESLRELPDIDWGKVNVFHMDEYIGLPEGHTASFPYFLRKHFLD
                     YVKVKNFYPVPGNPKTLREDMLAYEKLLRENPADLCALGIGENGHIAFNDPPFADFDD
                     PVYIKKVRLDERSRKQQVGEGHFHSLDEVPMYALTLTIPALRSAKHMLCMVPESRKAE
                     AVYRTKTEPINENCPATILKDTPWCTLYLDKDAAEKILPK"
     gene            77565..78200
                     /locus_tag="PK898_00365"
     CDS             77565..78200
                     /locus_tag="PK898_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOU18514.1"
                     /translation="MKKQLFILGDSISIDYTPVLREYLGSEWEVLRKGDIPAPEISGE
                     IDHENGTDSNVVYEYLRLVLPFIQASLILLNSGLHDIKRMPNETDECQVSIERYRKNI
                     EKIIHLIQSEKKQALWVTSTPVDDQQHRKYETSFFRRNDDLVRYNRTAIEIMKKNNIP
                     VFDLYNFTSRINAPLYRDHIHFNNEIINLQAAFLSGCIQTFFSYGTRNDEE"
     gene            78201..79352
                     /locus_tag="PK898_00370"
     CDS             78201..79352
                     /locus_tag="PK898_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU18515.1"
                     /translation="MNSRERLITTMNFKEPDRVPFDMGSTQVTGINEQAYRRLRSAYG
                     LPEKEVVYSDQIQGLALPDDDFIEALGIDIRGLFPLNAHNWNVKVTDGGKYWLYLDEW
                     GITHRKPKENGLYFSIYQEPLKNEKLSPDSIKNHPWPNFKDPQRIAGLKELADLYHSK
                     NYGVVMKDPFAGIFEMSQRICGMDYLLMLMASEPDLAGLLFDKMVELKIDFFSSALPV
                     LADSVDVIALMDDYGTQVSQLISPRMFRNQIKPRWKEVFDCIKQYAPNAKRFFHSCGN
                     VRPLIPDFIEIGVQILNPIHVKATGMDPYQLKKDYGDQLVFWGGGVDTQGILPMGTPE
                     EVREDVKRNLDALMPGGGYVFNTVHNIQADVPTENLVAMIETFKEYGVY"
     gene            79359..80435
                     /locus_tag="PK898_00375"
     CDS             79359..80435
                     /locus_tag="PK898_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU18516.1"
                     /translation="MLKSDQFLPLEFVFNPNWWNKTAGISFDRDFYLNPKKREENDVM
                     MRRILFEKFGEFGFGEENPKPRPIAGSMMVAGGFVIPALLGAEIRFSAAEAPQPLPQI
                     LSKDQIMSFVEPDILSNWPMNELADGWDRQQKEYGYLLGDINTDGILNAAYHFYGPDL
                     FADLLEDPDVPVRFMRKIAKIIIQTATYVKNRTGTTSVSVNRMACRLSPSPFIHANCS
                     VQMISPRTYEKNLLPIEQDMAKSISDYGVHHCGVKTEIYAKFYNQIPVCYCEIGWESN
                     PEKCRSFLPNAFLNLRLSPIRMLSCSSSEIKNDVEELLNSVGDLTNVGVSCINMDFGT
                     PEENIMTVSETVERYRILWKKHHG"
     gene            80428..81465
                     /locus_tag="PK898_00380"
     CDS             80428..81465
                     /locus_tag="PK898_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU18517.1"
                     /translation="MDKLIQNFFQASSHQEVSKVPLALIADSPWIPGFAGIDTLDYYL
                     DQKKWFEINLNLRERFPEVVWVPGFWWEYGMAIEASAFGSRIIFHHDQPPSMEPISTD
                     LQFWKNKTKVRDPEKDGLMPLALRQIERSDHELQSYGLGQHIASSRGILTVTSWFMGV
                     TTLMESLLSAPDDIIPILEVVTDTIILWLEAQLKRMREPKGILLLDDLIGMISKRVYL
                     RIVDPLFRKIWDHFDGYVKIYHNDTSCEHLYPVLAETGFDVFNFSDKTDIGTARQLMG
                     KQIVLMGNVAPRDIGALGTPEQVYQAAWNCLEKTNGEGLILSFGGGISPGTPAENIDS
                     MIKALNDWYKR"
     gene            81516..82940
                     /gene="uxaC"
                     /locus_tag="PK898_00385"
     CDS             81516..82940
                     /gene="uxaC"
                     /locus_tag="PK898_00385"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOU18518.1"
                     /translation="MSKKWNLPEDRFFDNEPAQKRIALEIYERIKNLPIVSPHGHVDP
                     KLFANDSSGFGTPSDLLVIPDHYIFRLFYSQGLKLEDFGIPYRNGLQDASEKDHRKIW
                     QHFADNFHLLRTTASYTWLAYEFKFIFDIDEIPDSENAMRIYDNLTEKLSSPEFQPRE
                     LYKKMNIEVLCTTDAASDSLKTHQEIKESGWDGKIIPTFRPDAVTNIRGENFRQEIEK
                     LSAAADIDIHDFESYIRALEKRRAFFKEMGAVSTDQAVITPYTHELPQSETDRIFTAA
                     LNGNVSDSDATAFEGHMLIEMARMSCNDQLVMQLHPGVLRNHNRIIYDKFGPDKGCDI
                     PIQTEFTKNLHSMLNQYGNNPNFTLCLFTVDENTYSRELAPLAGHYPSVRLGPPWWFN
                     DSWNGMTRFFDSVTETAGLYNLSGFVDDTRAFPSIPARHDVWRRVVSNWLAKMVVRSF
                     IDVNDAYDFAFLLAYSQPKNVYKL"
     gene            82965..84386
                     /locus_tag="PK898_00390"
     CDS             82965..84386
                     /locus_tag="PK898_00390"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOU18519.1"
                     /translation="MKYSLINTSVTTIDLQKIWMIRDAEGNRYLQSDRPIKALKSENN
                     DNIYLLSPENAKWLRSQIPWLNPQPLGLKTSVGTGDRLGISTPGHIRAFQGTQISPVL
                     AQQSVRELIRTHRTAQMVLDDAMWGVFEAGWNRPWGADADHLKTIDDMREYYEAGFTF
                     FTIDPGEYVKNLNSLDNIPLQSLLNSIDYDLLATSESELINNYTHFPDSLDFITASEQ
                     DIKRAFIKYGGAIHHVQKMYFQLKSWMGNKPFDFEVSVDETDEPTTIFEHYFIACELK
                     RLGIEWTSLAPRFVGKFEKGVDYQGDLHEFESEYRKHAAIQKYFGNYKISIHSGSDKF
                     SIYKICAVNSEYKTHLKTAGTSYLEALRIIATVDPELFRKIYDLSMNRYEIDKASYYV
                     SAHPENLPKISSLADDRLPDLLNQFDSRQVLHVCFGAVLDQYGTDILRDLNRNYDLYL
                     DDLEKHLRKHLQDFIYSKPAEES"
     gene            84394..85665
                     /locus_tag="PK898_00395"
     CDS             84394..85665
                     /locus_tag="PK898_00395"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOU18520.1"
                     /translation="MTFSNEVFGKGSPDPKDFLTESDIKKILTDFFSANNLSGKKVLV
                     IIPDSTRTMPLPLFFKLFYELIHDEVKTLDFMIALGTHPPMSDEAIANEIGISIEEMK
                     GKYQDISFFNHHWDDPKTFIELGEFPKNEVIELTKGTVQRAIPIQINKIILNYDALIV
                     LGPVFPHEVMGFSGGSKYFFPGISGPDLTNTTHWIGALHTCIKTIGLKITPQRQLIER
                     AANLIPVPKLYCCPVVKKEGIFGFYAGEPYRTWEKAVDLSSQVHVKYVDKPFKKVISV
                     MPHLYDDIWTAGKGMYKLEPVVEDGGELIIYAPHITEISYTHGKTLDRIGYHCLDYFL
                     KQWEKFKDEPWGILAHSTHVTGLGTYENGIEKKRIKVSLATGIPKERCERVDLGYVNP
                     QELNLQEYEHKEHEGILLVPYAGETLYRLKQ"
     gene            85720..86538
                     /locus_tag="PK898_00400"
     CDS             85720..86538
                     /locus_tag="PK898_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU18521.1"
                     /translation="MLPDFSVKGKVVVITGGGGAICGMMSKMFGEAGAKVAVMDISMD
                     AAKKITDEINNKGGESMPLVCNVLDKKSIEDAAAQVMGKWGRVDILINGAGGNKPQAT
                     TNNEMSFFDLPADAIQWVFNLNCLGTIIASQVFGKIMAEQKEGCILNISSMNAFRPLT
                     RIPAYSAAKAAINNFTQWLAVDLAKNFSPNIRVNAIAPGFFVGNQNRDLLIDKNSGDL
                     TQRGKTIISLTPMNRFGEFEDLLGATLFLVSPASSFITGVVLPIDGGFSSFSGV"
     gene            86567..87463
                     /locus_tag="PK898_00405"
     CDS             86567..87463
                     /locus_tag="PK898_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOU18522.1"
                     /translation="MSELSNQAKELLNFKPKHEFFVGIDSDGCVFDSMEIKHKECFCP
                     NTIRVWGLQPISKYAREAVEFVNLYSKWRGINRWPALIKVFELLSERPEVIRRGAVIP
                     KGDDIRAFINSGLPLSDAGLKKYMETHSSEELDRALEWTTSVNRTVKEIVRGLKPFPY
                     AKESLQAIHPFTDAFCVSATPADALEREWKDGEIRDYVEMICGQEIGTKEYILRLGSA
                     GKYPSDKKLMIGDAPGDLEAAQAVGALFYPINPGHEEESWEKFYKEGYKRFLEGTYSG
                     EYEKRLIEDFEKLLPEIPPWKR"
     gene            87517..88929
                     /gene="gndA"
                     /locus_tag="PK898_00410"
     CDS             87517..88929
                     /gene="gndA"
                     /locus_tag="PK898_00410"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOU18523.1"
                     /translation="MEKAKIGLIGLGVMGANLSLNIERNGFPVAVYNRTVEKTHNFIK
                     GAAAGKKIYGVETLKDLVDILERPRKIILMVKAGSPVDETIEAIKPYLEKGDILIDAG
                     NSFFQDTERRSEMLAKDGINFFGMGVSGGEEGALWGPSIMPGGQRECWEELRPVLTAI
                     AAKYKDGEPCVAYIGPRGSGHYVKMVHNGIEYGDMQLIAEIYDLLHRGLGLGQQRLQE
                     IFSNWNSGVLKSYLIEITAKILSKIDPETGKPLVDVILDEAQQKGTGKWTAQNALDVG
                     APTPTLTSGVEGRILSSLKEERVVASKILKGPDLTFYGDREEIIRAAENALYCSKIAS
                     YAQGLGLLKIAGKEYDYNLNLGEIAKIWREGCIIRADFLNDITGAFNKNPSLQNLMLD
                     DFFRSAIETRQSDWRLIAKTAIDLGIPMLATNASLAYYDSYRSETLPANLTQAQRDFF
                     GAHTYRRIDKEGSFHTIWEE"
     gene            88931..90214
                     /locus_tag="PK898_00415"
     CDS             88931..90214
                     /locus_tag="PK898_00415"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOU18524.1"
                     /translation="MKKNVIVSQSGGPSPVINSTLRGIVEMCRDIPDKFGEVYAGYHG
                     IEGVLKEELINLSAQNEEEIEYLSVTPAAGGIGTCRYKLRSSQKEDFDRVIDVFRAHN
                     IGYFFYIGGNDSMDTANKISLLANERGLDLVVVGGPKTIDNDVGDSEFKLIDHTPGYG
                     SVARYWALNIQNANEENEGSSPADPILVLQAMGRKIGFIPAAARLADPKREMPLQIYM
                     TESKVTLPELADNVNEELKRSKRCIVVISEGFDVGDIGARRDNFGHISFGSSQITVTQ
                     LVVNYLNEHGLKSKGLARGDMPGTDQRRNMIYASTVDMEEAYKLGQQATLIAASGETG
                     YMSTILRNPGPIYNVRYDKVPLDLVANSERFFPEAWIAPNRMDVTDDFVRYARPLIGD
                     NWPAIPLVNGIQRFARIEQIFVPQVLPEYVPEASR"
     gene            90314..90949
                     /locus_tag="PK898_00420"
     CDS             90314..90949
                     /locus_tag="PK898_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOU18525.1"
                     /translation="MSDLLNEIKEAIIDGSAKIVSEKVTAALEEGFDPEQILNDAMIA
                     GMAEVGQLFEDGEYFVPEMLVAARAMTTGMQILKPRLVQADVKAAGKVAIGTVQGDLH
                     DIGKNLVGMMLEGASFEIIDLGADVPPEKFVEAVQKDHVQIVAMSALLTTTMPSMKTT
                     IEALEAAGVRDQVKVMVGGAPLSDQYAREIHADGYAPDANKAVALAKSLVS"
     gene            complement(91027..91233)
                     /locus_tag="PK898_00425"
     CDS             complement(91027..91233)
                     /locus_tag="PK898_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18526.1"
                     /translation="MRKSLSIVLLVVLLASIIAGCASSTATPKPTEVPSEAAPSEAAP
                     AEASPAVEATPTTQASISYSWFMM"
     gene            complement(91322..92521)
                     /locus_tag="PK898_00430"
     CDS             complement(91322..92521)
                     /locus_tag="PK898_00430"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOU18527.1"
                     /translation="MEKIQMKNPIVELDGDEMTRVIWKWIKDELILPYVDLKVEYYDL
                     GLPARDETDDRITVDAANAIKQYGVGVKCATITPNEKRMEEYKLKKMWKSPNATIRAI
                     LDGTVFRTPILIESIKPVVRTWKKPITIARHAYGDVYKGTEFRIPGKGKAELLFTAED
                     GSEMRDTIYDFESPGVLQGIYNKDSSISSFAHSCFRYALNSKQNLWFSAKDTISKKYD
                     GTFRVIFEKIYEDNYRRNFEDEGITYFYTLIDDAVARVMRSEGGFIWACKNYDGDVMS
                     DMISTAFGSLAMMTSVLVSPDGNYEFEAAHGTVTRHYYRYLNGEETSTNPTAMIFAWT
                     GALAKRGEMDNIPELQEFAKKLEKATLDTLEEGICTKDLADLYEGVTSHPVSTKNFLR
                     EIQRQLM"
     gene            complement(92553..94832)
                     /locus_tag="PK898_00435"
     CDS             complement(92553..94832)
                     /locus_tag="PK898_00435"
                     /inference="COORDINATES: protein motif:HMM:NF008503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HOU18528.1"
                     /translation="MIELIDKEIIYSETYGFVQKTDERLADFMKESGLKEISELRRNT
                     IAYQILEKHNHSKNPAQMQLVFDSLASHDITYVGIIQSASASGMKEFPVPYTLTNCHN
                     SLCAVGGTINEDDHVFGLSAAKKYGGIYVPANLAVIHQFEREMMAACGSMILGSDSHT
                     RYGALGTLGIGEGGPELVKQLLQKTYDIDAPKVVLVYLENKPNPWVGPQDVAIALIKA
                     VFPTGFVKNKILEFAGPGIQYLPMDFRNGIDVMTTETACLSSIWQTDESVKTYFELHG
                     RPEAYRPLHPENYAYYDSMVHIDLSKIEPMIALPFHPSNGYTIRELLENPGDILRSVE
                     TESEKIFGKASILKLTDKIHDGRILADQAIIAGCAGGMFDNLCAAADILDHGIVGNDY
                     FSMSVYPASMPINAALTENGVMLRLQKSGVVTKMPFCGPCFGAGDIPANNGFSIRHTT
                     RNFPNRDGSKPDQGQVTGVALMDARSIAATAANSGYITSAADMDIEYKSYPYHFDSEI
                     YRKRVYCGYKKPQKDVELRYSPNITPWPEMYPLAENLLLVLAAVIRDPVTTTDELIPS
                     GETSSYRSNPVKLADFTLSRRVPEYVNRAKKIRDLESERRKGSKLPKFEEILDKLNHP
                     NGANNLNTAIGSVIFANKPGDGSAREQAASSQKVLGGLANIAYEYATKRYRSNCINWG
                     IIPFTIDPEEPFEYQPGDKIFIPGIRKAIAGGIESIPAKIITGSGIKDILLHLKGLTR
                     EEREIIISGSLINFYARKK"
     gene            complement(94963..95433)
                     /gene="bcp"
                     /locus_tag="PK898_00440"
     CDS             complement(94963..95433)
                     /gene="bcp"
                     /locus_tag="PK898_00440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOU18529.1"
                     /translation="MTELKVGMPAPNFTLPDQMGKIHSLSDFRGKKVVLYFYPKDNSA
                     GCTRQAIGFRDLYEEYMRLAVEIIGISKDSEKSHQKFIESHALPFLLLSDPELNVIKA
                     YDVWKEKNLYGKVTMGIVRTTFIIDEDGIIIKIFQKANANTNAKEVLEYLQSIR"
     gene            95679..96335
                     /locus_tag="PK898_00445"
     CDS             95679..96335
                     /locus_tag="PK898_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOU18530.1"
                     /translation="MEKVVINAEKRDVVGKKVGSLRRQGLIPGVMYGHNEPPFPITMN
                     AREIERVINNLTSSSIVTVKVNGESHQALIREKQKDYIRNQIIHIDFQILSLKEKIRS
                     KIEVKLVGVAPAVKNFNGIVLQEREFIDVEALPADLPERITVDISGLENIGDLIRVGD
                     LDISDAVTVFDDVNDVIVSISGAMAEEAVEEEVTTAEGTEPEVVEKGKKESEEEEEKK
                     "
     gene            complement(96407..97216)
                     /locus_tag="PK898_00450"
     CDS             complement(96407..97216)
                     /locus_tag="PK898_00450"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOU18531.1"
                     /translation="MNESWDEKQLIKEGEIAEIVGISHFHHIIKIQADQEIQTHKGII
                     YTNDFIGRPWGSIIHSHNGNKFYVIQPALTDMIKNIPRETQIVYPKDVGFILLNLAIG
                     PGKRIIEAGTGSGALTTAFAYAAGSTGKVYSYDSRADFQKLAEENLKRVALDEQVEFI
                     TRDIRLGFTEKNVDALFLDIPDPYNYLAQVRDALKPGGFFGCLLPTANQVILLLEQMN
                     ANQFGFTEVCETGIRYYQTEAERFRPVDRMIAHTGFLIFGRPLIKDEDLMV"
     gene            97318..98154
                     /locus_tag="PK898_00455"
     CDS             97318..98154
                     /locus_tag="PK898_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOU18532.1"
                     /translation="MKKGKLFIVAIVSLSVLSVLLSGFISVKPQDKQEFKVGLECNYA
                     PFNWTQLDDSNGAIKIKGGGGYCNGYDVAIAKIIADGLNRELVLVKTEWDGLIPALNA
                     GKIDAVIAGMSPTKERKASVDFSDPYYESDLVIVVKKDSPYANAKSLQDFSGARITAQ
                     LNTFHYTVIDQIPGVKKQTAMESFPAMIVALSSGKIDGYISERPGAISAMESNPDLTF
                     VEFEEGKGFETSPEDVAIAVALKKGNPDLEAINKIISTITKEKRQEIMDNAIAIQPLS
                     GE"
     gene            98277..99047
                     /locus_tag="PK898_00460"
     CDS             98277..99047
                     /locus_tag="PK898_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOU18533.1"
                     /translation="MPSTFLEWVRFFVNEYGLYFLQGALTTLYIALIGTIVGFLIGLA
                     VAVVRTIPIEENDSFGRKIFYKVVRWILVAYIEIFRGTPMIVQSMVIYYGSAELWGLD
                     MSPIVAALFIVSINTGAYMAEIARGGIISIDKGQYEAALSIGMNHWQTMEKIILPQAI
                     RNILPSIGNEFVVNIKDTSVLNVISVTELFFMSKSAAGTYMRYFEVFFITCVIYFVLT
                     FSVTRILLAVEKKLEGPETYIIHGSQTVPEAVIKGEIR"
     gene            99056..99805
                     /locus_tag="PK898_00465"
     CDS             99056..99805
                     /locus_tag="PK898_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOU18534.1"
                     /translation="MISVNHLQKNFGENQVLKDIDFYVKKGEIISIIGSSGSGKSTLL
                     RCINLLEKPTSGTISFHGKDIQSKGFSLSEYRSKVGMVFQTFNLFNNLTVLENCVVGQ
                     MSVSKIDRKTAEEKALRILASVGMAQYIDAKPKQISGGQKQRVAIARALAMDPEVLLF
                     DEPTSALDPEMVGEVLNVIRDLAISGLTMLIVTHEMNFAKDVSSRVIFMDDGVIVEDS
                     SPEKIFSDPENDRTRAFLKRILNPEKDPGLQ"
     gene            99837..101717
                     /locus_tag="PK898_00470"
     CDS             99837..101717
                     /locus_tag="PK898_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18535.1"
                     /translation="MKKEKFIAFPLFLILAIAAIWVTLFKPIPRQIGLIFRQEIYILP
                     LIFLLALLPSIQKSAAWQAVCYAVLFTVCLLPLSGLWNSGISDQYIFGSSIPWSDAFT
                     HHLNTLRFLYGQTMGQSTAVRPLSVSFYSFILYITNNNFIILMSSLAVLSAFVMLINF
                     RLIGIHIGPVAAAFFFTNAFFFIRKFLGIYMTEPYAFIIGLLACYFLLNGIFKKQNGQ
                     ILLGFCLFSLAMNARPGPMFTIPLIGLWYFFIFLKGHHRRIICATVAFIALLSGFVIN
                     QWNVNRVYRSDKVPNLQAAQIIYGLCLGGKDSYYVMGLPEINELNKSENIYKDVFGLC
                     SNVLKEHPENIGVSFNAIWNTLLFDSNRGSFSYFDGTENKMLIDLIRMGLMVLWSIGI
                     FLIFRQRKQPFGSFMIAMVLGLILSQFVVAPYTTFRMRYHAATIWVPGLIIGIFPQYV
                     INRFAWKNLKRESESTPVTGSTSLALALSGFFILIALIAPELIGTRTLKPPAKPEISC
                     KKGETTLYTKIDQGSYFYMENKSELQQEHYPYFRLPFVRQHFHDTASVEMFDFTDRID
                     QPTAIIRGIDLSDWKDALIFSPLPLVKDRIGYVVFCGNYLDPPILRQDRFFIPSSAYF
                     LQ"
     gene            101738..102301
                     /locus_tag="PK898_00475"
     CDS             101738..102301
                     /locus_tag="PK898_00475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU18536.1"
                     /translation="MTKFILLRHGQTDWNIQRLYQGQEDIPLNETGKRQAEEAAETLK
                     NVPIDIAYCSDLQRAVETAKIVLRYHPSVPLMIDKRIRERSFGEYEGKPYQKDLMNPT
                     DSNEINKKRDSLNFKFPQGESLLDVYERANSFYNDILQKHPNETVLIVSHGTFLSILS
                     AIIYQDELAERKKYIFNNAEPVFIDPT"
     gene            complement(102307..103008)
                     /locus_tag="PK898_00480"
     CDS             complement(102307..103008)
                     /locus_tag="PK898_00480"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOU18537.1"
                     /translation="MKLAVLQSNYLPWKGYFDIIHDVDLFLFYDEVQYTKNDWRNRNK
                     IYTATGLKWITVPVYGTTEKRIDEIEIVRGRWQMQHYQAILTNYAKAPYFRQYRDFLD
                     FVYLDTVWNSLSELNQFLIRKIATDFLHISTKLDRSTSYSSAGSGHQKLLSLVKNTGA
                     NSYESGPAAQSYIISSDYREAGIELIWKDYSGYPQYQQLHDPFQHQVSILDVLFNTGP
                     DAPYFIWGWREEVKN"
     gene            complement(103015..104154)
                     /gene="rffA"
                     /locus_tag="PK898_00485"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(103015..104154)
                     /gene="rffA"
                     /locus_tag="PK898_00485"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOU18538.1"
                     /translation="MISFNVPPYIGEEEKYIEKAIQNHKISGDGEFTHLCSSWLEEKT
                     GAKKVLLTTSCSDALEMAALLCDIREGDEIIMPSFTFVSTANAFVLRGAKIVFVDIRP
                     DTLNIDESLIENAITNRTKAIVPVHYAGIGCEMNTIVKIAEKYHLMIIEDAAQGVMSS
                     YEGKALGSIGDFGCYSFHETKNYSMGEGGAILIRDESFIEKAEIIREKGTNRSKFWRG
                     QVDKYTWVDKGSSFLPSELNAAYLYPQLLNAEKINQNRLGSWNEYYEQLKPLADAGFI
                     ELPSIPPECVHNAHLFYIKTNSLSERTKLIQYLKESKIETVFHYIPLHSSPAGKRFGR
                     FQGEDRFTTSISERLLRLPMYYGLSSEDIATVTQSIMSFYRFKRN"
     gene            104327..105634
                     /gene="lysA"
                     /locus_tag="PK898_00490"
     CDS             104327..105634
                     /gene="lysA"
                     /locus_tag="PK898_00490"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU18539.1"
                     /translation="MTDSDLKRLYPISATINSKNHFTIAGSDLSDLAESYRTPFYIYD
                     RDTIHENIHRIQSAMADFYSGPGTIAYASKAYLSRKFASKIATEEVDLDVVSLTELRF
                     ALAAGFSPDRIHFHGNNKSIEEIEIAVNQNIHAIVVDNMDELLLTERIANSKKKVAQI
                     WLRITPDIRVATHPHIETSAATSKFGFHIINGDAANAIEYAVNSSALCLTGLHCHLGS
                     QLFDPLPYQIAIEKMFDLAINCHYIPLELSPGGGWGVPYVEDDPDSNPEQWVKAISKT
                     ITEKCNCYQIRLPKLILESGRFIVARSGVAIYQIGTQKKSVNGERIIAVDGGMSDNPR
                     NALYQAKYTATPVDQSVHSRKNPCKVVGKYCESGDVLIDNVMLPDLNYGDLLMIPVAG
                     AYQLSMASNYNLAPRPVVLWLEPEGKVEVLQKRENLEQNSWWD"
     gene            105796..106866
                     /gene="serC"
                     /locus_tag="PK898_00495"
     CDS             105796..106866
                     /gene="serC"
                     /locus_tag="PK898_00495"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOU18540.1"
                     /translation="MKKHNFNAGPAILPDYTYEKCIEAIKDFDNTGLSILSVSHRSKE
                     FDAVMDNAVAATKRLLDIPSGYSVIFLGGGASTQFYTVPYNFLGKKAAYLDTGTWAMG
                     GIKEAKLFGEVDIVASSKDKNYSYIPKNYTVPEDADYFEICSNNTIEGTEIREDFDCK
                     VPLVADMSSDIFSRPVDVSKYSLIFGGAQKNFAPAGVTIVIIKDSFMDKVVRPVPTMA
                     NYKVHVENNSMKNTPPVFPIFAGLKTMEWMEQSGGVKEMEKRAIARSSKIYDALDSSK
                     LFKATVEDPQDRSRMNICFVLKPEYSELETAFLDLAKQRGLVGIKGHRSVGGFRASCY
                     NALPMESVDALIDTIHEFEKAH"
     gene            106889..107812
                     /locus_tag="PK898_00500"
     CDS             106889..107812
                     /locus_tag="PK898_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU18541.1"
                     /translation="MKKVLIATEKPFSKAAVEKIKSILESGDITVKLLEKYTDVNQLY
                     EAVADVNGLIVRSDEITKDVIDHAPNLEIIVRAGAGYDNIDLQAAKERGIVVENTPGQ
                     NSNAVAELVAGMMIMNARNFYDGSTGTELKGKKLGIQGFGAVGKCLARIANGLGMYVH
                     FYDIFVPFEAGYEYNVFPVEDIKYLYKTCQYVSLHIPALPSTIKSINYDLMSLMPQGA
                     VLINSARAEIIDEDDLLKMFETRPDFHYLADVAPSNKAVIEEKFPGRFFFTPKKCGAQ
                     TSEANNNAGTAAANQIVSFFLTGDKKYQVNK"
     gene            107911..108597
                     /locus_tag="PK898_00505"
     CDS             107911..108597
                     /locus_tag="PK898_00505"
                     /inference="COORDINATES: protein motif:HMM:NF022970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HOU18542.1"
                     /translation="MEPITIGNIVRVNHSFIDEDSAINLKGHEGVVRMIFSPEENDPD
                     ETLYLIEWTEKTLKQLPDDYYIKLFTDEKEWSYKIVPEHILLKVEGEYNADAVEWEKN
                     AIGARLFWQYFGRSGKLIQSLFPNDKEKTDRSPYDTWLKYLNQNLRFPFEATIFYEYE
                     PDDGILQPSDSVNVQGLAGWDYPLGIFCEVQFKGKYFILPLQDIGMTNELSKNARILN
                     AYEAWLLCRA"
     gene            108585..109562
                     /locus_tag="PK898_00510"
     CDS             108585..109562
                     /locus_tag="PK898_00510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOU18543.1"
                     /translation="MSSLNSSNFFIGDIPVRGKTILAPMDGYSDSPFRQITRKLGSAA
                     SITEFVNTMEVVSRNTKYEERCSFQPSERPIGIQLFDNNPDRIIRGAEIIVNKWNPDW
                     IDINMGCSISHVANRGAGVGLLKDPHLVAEIISGLTGRLSVPITAKIRLGWDNESINF
                     LEIGKILQDNGAAMVTLHARTGKQLFSGNPNWDAIALLKQTLQIPVIGNGDIKSVSEI
                     KSMLDYTGCDAVMIGRAALQNPWIFAGYDADEVPLFLFQEICLEHLSLMKSFYGDTRG
                     CILFRKYAKKYLSHTGLEKHLIRDLLTTSDPDLFTEKFTDLIISTAQLI"
     gene            complement(110093..111406)
                     /locus_tag="PK898_00515"
     CDS             complement(110093..111406)
                     /locus_tag="PK898_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18544.1"
                     /translation="MSSDKEKIKQIFNIFWPLAISWLFMAVDIPFVSAIVSRMPQPEI
                     NLAAHSVVYPIALIIEAPIIMMLSVSLALSKDKKNYRRIYKFMMILCAGLTIIHASIS
                     CTPLFYLVVGNLMGAPKELLPYARVEMIMMIPWTWAIGYRRFYQGILIRNGHSKQVTI
                     GTIIRMSFLIGVLLIGYRLQDFVSGTMTAGAALSSGVIAEGLFCGLKGRKTADRDFEE
                     GELSELISWGELIRFVIPLILTQIINLGWMSLGSAAMSRMINPIASLAVWPVLSGLLN
                     IIKSFGSACNETTLSVINREGFYKADRRFTFYIAIFSTLFYAALIVTPLGRFWFEGLS
                     SLTPELSDLAKKALPMIFLVPFVNTYLNFYQAILLAGKKTSGFLESLLIYLIFMISIL
                     YIGILTQKWEGIYVIMIGMNIAVLAQTLWTRFRAKKIIPLFDHPK"
     gene            complement(111598..112272)
                     /locus_tag="PK898_00520"
     CDS             complement(111598..112272)
                     /locus_tag="PK898_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOU18545.1"
                     /translation="MKKSTVDDEYPNSSKRLFVPAVETKAEITVINSKFIATCAPVFS
                     VEEAKDFILKIRKTYPDATHHVPAYVIGHHSSTVCYCNDDGEPAGSAGRPILTVLTGS
                     GMGDIAMVVTRYFGGTKLGIGGLVHAYGGAAKKVLSIVPRAEKVLMDSFRFDIDYHLF
                     ELIKNLFNTENVLLIDHIFAEKITISAKVESFRFPEIEEKLNELSNGKIILEKISERK
                     TEIRPI"
     gene            complement(112293..112706)
                     /locus_tag="PK898_00525"
     CDS             complement(112293..112706)
                     /locus_tag="PK898_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18546.1"
                     /translation="MSRVIRTGSASTERNRLKKALVLAIRELMKQSEPNETSRDLIAF
                     ILLCLERITETVEESAEAWEKRGYWLKADHFRMEWEWARLLAERIRPNVLTENYNKII
                     PDLIELSQSLNDVTISDNHRLGTPWLGAWDQLHKK"
     gene            113050..114378
                     /locus_tag="PK898_00530"
     CDS             113050..114378
                     /locus_tag="PK898_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOU18547.1"
                     /translation="MEEIKTLSSIPLELKYQESLIQVNPEQFSFKIDIEKAQSELESK
                     TSEMCSSKTFLNSLFREQKSEPISIALESNADEEKIESFLRNEISARYDQPAAAKYPD
                     LNGSGFYSGHPGYQLDIKKAVKMIQEALTSFDHQTIDLPIISIPEPPSSLTNLEILLS
                     SVVDQNQDPDQITEIYLLDPKTGEEFDFARQNKSNLIPEIAFTAASTIKIPIMISAFK
                     HLDEKPSAVVSRQLELMITESKNEQTDWLLENVIGGETAPLEVTKDIRALGFQNTFLA
                     GYFYLGAPLLDRIETPANTRTDIDLKPDIYNQTTPKDIGLLLYLIYQCDLYGKGKLTE
                     VFPNEITQSECSDMMILLKNNHLPYLISAGIPDGIPIAHKHGWIEESDGLLHTMSNVA
                     AVYSPGGDYILSIYTYHPQNLIFEKGNILFSRISSAVYGFFNPQQNDTVN"
     gene            complement(114392..115045)
                     /locus_tag="PK898_00535"
     CDS             complement(114392..115045)
                     /locus_tag="PK898_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU18548.1"
                     /translation="MNKIRIVLVDDHEVVRLGLKSLLEQGSRFEVVGEASNAKEAIEI
                     TGKLRPDIVLMDIRLPGQSGIDACEEITQKYPDVKVVMLTSYAEDEMLFSAIRAGASG
                     YLLKQISSEDLVRSLESVARGDALLDPLVTQRVFQEVRRAVKEEEASAFAILSQQEKH
                     VLILVSEGKTNREIAKSLFLGEGTVRNYVSSILSKLGTSNRAEAAAYAVEHNLKDYL"
     gene            115175..116407
                     /locus_tag="PK898_00540"
     CDS             115175..116407
                     /locus_tag="PK898_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOU18549.1"
                     /translation="MQGNAFKVAILGGGPAGIAAALMIRHISDYDVYLFDANPKIGRK
                     LSVTGSGRCNLTNLELDPERYYSTQPADLSPVFSRWSPADLRLFLDRIGIPTIATDDG
                     WVYPQSFSAANVVQILNGNLTENNIHIIENTKVLSITLYENQFRLTVDNRKDSLVFDR
                     ILIATGSKAFPQLRADTGIVSELKNFGIHVAPFFPALSPVLLENEPHKLLSGIRLDVN
                     LKLFRESRQIDESFGNIIFTDFGMNGPGCMNLSHRIQLLLENHPYFKVDFIPQEKSKI
                     IEGIFNSKQDQNYPYRDIFLSILPMKLVDFFFNEWNIATDTKCSEINHQSFGSHIDQL
                     RNFKMQIKGTKGFKESQACSGGVLLSEIDLRTMESLKIPGIYFAGEILDVVGPCGGYN
                     LHWAFSSGLIAGEAMGKP"
     gene            complement(116408..118267)
                     /gene="typA"
                     /locus_tag="PK898_00545"
     CDS             complement(116408..118267)
                     /gene="typA"
                     /locus_tag="PK898_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOU18550.1"
                     /translation="MKTIRNDLRNIAIIAHVDHGKTTLVDAMLKQAKVFRANQQVEER
                     VMDSNDLERERGITILSKNTAISIPDPETGEMVKINIVDTPGHADFGGEVERVMNMVD
                     GALLLVDAAEGPMAQTRFVLKKALAMGLRVIVVINKMDRKDADPERTLNKIFDLFIDL
                     GATDEQADFPIIYADGIKGKAGYTPELSENLQPLFQTILRKINPPEVEIDQPFQMVVS
                     NIFYDEYRGLGVIGRIHRGKLKAGMNLSRIKPDGTIVPENARYLYTYSGLNKVETQEA
                     SAGEIISIAGLNEISIGDTLSDPNYPEALPVISVEEPTVKMTFGVNSSPFAGREGKFS
                     TSRKLRERLFSELRNNVALRVEETDTTDNFVVSGRGELHLAILIETMRREGYEFQVSR
                     PEVIMIEGSNGELLEPFEEVYIDTSPDTVGTVVEMLGSRKGRIQEITDNPDSSVHLHY
                     IVPTRGLLGFRYQFLTATHGMGVINSSFLEYAPYAGPIASRNTNSIVAWEDGLTTTYG
                     LKNAEERGVLFIGPGVEVYEGMVVGESMRVTDLTVNVCKKKHLTNMRQSNKDIEVRLN
                     AVRQMSLDEAIEYLAEDELLEVTPTNFRIRKKILDTEERGKTQKREREKLSDL"
     gene            complement(118291..118926)
                     /locus_tag="PK898_00550"
     CDS             complement(118291..118926)
                     /locus_tag="PK898_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOU18551.1"
                     /translation="MEPDKIKTPFSFNKAAIIAIAAYIGVQMIADISSLQIVSIFGLA
                     LDAGTFIYPISFTLRDIAHKILGKEHTKTLIYSAAGINLFMAGYFFFVSVLPVDLSAG
                     GSENWIKVLTPVWRITIASIIAELCSELIDTEIYSWWVEKITRKHLWARVLISNGVSV
                     PVDSLLFAFIAFYGMIPLPAVWEIFIGNVVIKYLVTLVSVPLIYLGKETEQ"
     gene            119067..120263
                     /locus_tag="PK898_00555"
     CDS             119067..120263
                     /locus_tag="PK898_00555"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU18552.1"
                     /translation="MKKKVLILTADAGFGHRSAANAIASALSKETKFEIETRIENLLD
                     SPKTPAPLRNTQTGYDKIVTESPKFYEFSYESSDKRLPTNVAKIGLSAMLYGPFSKTM
                     KEFNPDVLVNTYPLYQGPAQTWYLLNEDAKKAISKSKKTDDDSKSSSRHKHIPMITII
                     TDLVSVHLIWMSTLPDIYCVATHEMKEQIAGFGVDRERILCTGIPVNPVFAEEKRSKR
                     ELREQLGWNPEKTTIIAIGSKRVSNMMDYLDLINHSGFNIQLIMVAGGDETLYQEMQN
                     TTWHVPAKIYNFTKELPVMMLASDIVVTKAGGLVTSESLAASLPMILIDVLPGQEEGN
                     ALYIQENNAGVHAKKPIDILHTLTHWFENDGYGLRKFTENARKIGKPRSSLDIADLIV
                     EKLSES"
     gene            120272..121288
                     /locus_tag="PK898_00560"
     CDS             120272..121288
                     /locus_tag="PK898_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU18553.1"
                     /translation="MKKNLFWIFVSVLFFSVFFNGTKVEAISFDKVVYLINGSLGDNS
                     FYDSGQAGIDQLKKDYGVEARTIECGFDAGKYEPSLDAAVHYADVIFVISYGYEDVLK
                     EYADKYPDKIFVNLDTVVENDEHTITSVDYIEEESAFLAGIVAALVTTSQEIPNVNPQ
                     KIIGAVGGDTDPVVQSFIFAYENGAKYIDPEVKNETKYLGDWEDTSKAKQATLQLYDM
                     GADIVFQIAAAAGMGVLQASGERGLYSIGVDTNQNAIVPGHVVASDIKDVGKSILNVY
                     KTIKDGTYKPGEVLSYGLESGTVDVVFENNNDVLPKSIIDKVSEVKQMIINGDLKVER
                     YVAE"
     gene            121430..122986
                     /locus_tag="PK898_00565"
     CDS             121430..122986
                     /locus_tag="PK898_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18554.1"
                     /translation="MTESVRRDDNLTSLIEINHLVKIFPPNVVALNDVSVDFQEGEIH
                     AIVGENGAGKSTLMKILYGMIPADSGEILYRGKPIRFANPGEAIRSKIGMVHQEILLI
                     PQYTVWENVILGMEPLGKFNRIHRDLARKQVQDRIDEFQFNLDPDAKVEDLSVAARQK
                     VEILKLLYRDVTFLILDEPTAVLTPQEIPQLFDELRRLRNNGHTIAFISHHLDEVLEL
                     SDRISVLRKGKKVATIPASQTSESLLAEMMVGREVVFESIKETVRPGKLIFRLQNIRL
                     QSPNKKKLLDDVNIEVKAGEIVGIAGVEGNGQLELVEVITGLATPDQGQLLINGVNLT
                     KASILERRKYMAFVPQDRALMGSCLNASIKENLIMTHYKLNPEFTRHGHILNDKKSTE
                     FAYEIREKFDIQLHSIEDEMKSLSGGNQQKVILGRELLLSAPFILLDQPTRGLDVGSI
                     EYVHQQIIRMSKENRAILLLSSDLEELFRITDRILVLHRGQIVANLITNETNINEVGY
                     LMLEGAAHGA"
     gene            122976..124022
                     /locus_tag="PK898_00570"
     CDS             122976..124022
                     /locus_tag="PK898_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18555.1"
                     /translation="MAHNLRNILINLAGILIAFLLGAVVMIFQGYNPLETYEALFGYS
                     LGNTYSFMTVLKNSVPLILTGLSASVAFASGPVNLGQPGQLLMGALFATVGGLYVDLP
                     SVLMIPFLLVSAMVGGALWSGIAGLLKRLFNMDEYITTLMMNMIADFFTYWAITYPFF
                     EKNANHPQTPRITKNGWMPEIGDFNTNFLVTLIVFVIIWFIFYRTTNGYEWRISGKNS
                     LFARLGGVEIEKNYMHVMLLTGALAGLAGGLIIMAGPHRFIKGLGANYAWDGIMVSMV
                     ANNGIVATLIYGIFFSILQTGALGMELITQVPSELITVLQAVIVLVVVASRGYFTLLL
                     SKMMTKRKLKERSA"
     gene            124019..124918
                     /locus_tag="PK898_00575"
     CDS             124019..124918
                     /locus_tag="PK898_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18556.1"
                     /translation="MTIFIGLINGMISAATPILLSALGGALTYYAGIFNIAMEGMMLS
                     GAFFAVLGSYYFGNWLIGMLFGILGALLIAGIFILFSVVLKVDEFVTGVGLNMFSVGL
                     TTYLLRQIFKVKGAFTDPGIYSVPKIRLPFIEKIPFVGDIISDQNLIVWIAFIATLLV
                     NYFVFKTQFGLRLRAAGYNRICLDTSGVSSNKIKTVSLLLCAVLCGFAGTYLSLGYVT
                     LFSENMSAGRGWISLAAVILVSGNPIGIALVSLLFGLADGLGLYLQHIFPSQFTSMLP
                     YIATLAALFFYSVNNKKKMEVKL"
     gene            124915..126291
                     /locus_tag="PK898_00580"
     CDS             124915..126291
                     /locus_tag="PK898_00580"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU18557.1"
                     /translation="MKAWEMEREIRDNAVPKDRRKNESHWYKSDMTIPYGDELIKKFW
                     KSNVPGSLAPEIPYLEMTQALSNQGYDVSAAENLIPEGIQLFEIGDIDNLRVLTAKLL
                     NALNNAPKIADHPYHQHEYPMSWEEIYRAMPISSQASSSRPEWKNTFKNKIYQGWIGQ
                     LAGGSFGTAIEGYIGSQIELIYGEVDSYITTPETTNDDVVYELAFLDAFAKNGRYVTS
                     QQVGLEWIKQIQFGWSAEWIAIRNLNMGIFPPESGRFLNFYSDWIGAQMRGMVCGMLA
                     PAWPVEAARLAFTDAVVSHSNNGVYGEIAAAVMTALSFEMENPRDIIQETLKYLPAKS
                     EYVEKYLIVLDILRTEEDPNIARGRIEPHFIRYNWIHSYPNMAADLYALWYGNGDFSE
                     SMKLLAKSGNDVDCNAGLVGNILGIIRDVPRKWSDPIGDLLETYIKGKERLSIRHLAE
                     KTSELAQQ"
     gene            126593..127486
                     /locus_tag="PK898_00585"
     CDS             126593..127486
                     /locus_tag="PK898_00585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU18558.1"
                     /translation="MFILWSLIDMMAKSLQSKLVLISGGSSGIGLATAKRMAREGASF
                     LILARDEVKLTNARELILREAVSNDQFVETLSVDMRNYEELRNKLVPILDRLGTPDYA
                     MHSAGVVYPALFEDITIEQFHWMMDTNYFAAVNLFQIIIPRMKARRSGHIIAMGSASS
                     FIAIWGYSAYSGSKYAIRGLMETLRCELKPFNIQTSIVFPPDTDTPQLAYENQFKPAI
                     TKEVSGTIKPLSADFVADKIYRGVMKNQYQIIPDRDTQLTYKAAHILGTGIFKVLDMV
                     TDKAIKKYGLESKNIYTEENK"
     gene            127483..128412
                     /gene="argF"
                     /locus_tag="PK898_00590"
     CDS             127483..128412
                     /gene="argF"
                     /locus_tag="PK898_00590"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOU18559.1"
                     /translation="MKKKDFLSIADFSESELIHILDVAQALKKELKEGGNKPILKGKS
                     MALVFQKPSLRTRVSFEMGMHQLGGYAFYLSPDEVGLGKREAVKDVAMVLAGYVDAIM
                     ARVFQHQHLIELAEFSGIPVINGLSDYNHPCQAMADALTIIEEFGKLKGINIAFIGDG
                     NNVAVSLMDIATKLGANFSIANPKGYDMPEEAISAAKEFAKLSGSRLVFSNDPYEIVK
                     NAQVIYTDTWISMGQESEAEERKKAFIPYQVNQKLVDAAPKDVIVMHCLPSHRGQEIT
                     DEVQDGPHSRVIPQAHNRLHAQKGIMAYLLADL"
     gene            complement(128483..128734)
                     /locus_tag="PK898_00595"
     CDS             complement(128483..128734)
                     /locus_tag="PK898_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOU18560.1"
                     /translation="MSNFFVILVSTFVNIMSILLLIYVVISWFVPPYNEFRMKLDQLM
                     NQFLDPIRRIMPTIGMFDFSPIILMLLLQFFESIVKIIF"
     gene            complement(128757..129482)
                     /locus_tag="PK898_00600"
     CDS             complement(128757..129482)
                     /locus_tag="PK898_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOU18561.1"
                     /translation="MTEINKEQIRNNLSEIQQKIDQAAAKSGRKSSKISLMAVTKKKS
                     AEVIRCLIDLNIRYFGENYPDETLGKIEAFKETEHAIRLCMIGHLQSRKINIVAEHFD
                     EYHSLDRLKTAVRLNQILKMINKTMPVFLEINIGNEDSKSGWSSENGNLSDQLLKDLE
                     VIRTLSQLKVDGLMALPPISENPEESRPYFVRLRYMLEKLNDNYGYSLHHLSMGTSID
                     YPIAIEEGATFVRIGTALTGPRI"
     gene            complement(129482..130276)
                     /gene="pgeF"
                     /locus_tag="PK898_00605"
     CDS             complement(129482..130276)
                     /gene="pgeF"
                     /locus_tag="PK898_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOU18562.1"
                     /translation="MTLVKESNGLRYYQFEEFAKLGVKHGFFTRLGGVSPVPFHSLNM
                     ATTVGDSNENVLENLNRMFQVFNLDLSTRYDGWQVHSSTTICVKSSRDVRTRTIRGDA
                     LLTDNPDVTLVMRFGDCVPVILMDPIFRVAGIYHAGWKGTVKKIGKNVVENMVKVYGC
                     NPQNIIAGIGPSIGPDHFVVQQDVSDQFIKAFPKDLDKIQIRAGDEVRYNLWEANELT
                     LSDAGVKTIKRMDICTVCHKDEWFSHRGDHGKTGRFGVLISMAGME"
     gene            complement(130273..130908)
                     /locus_tag="PK898_00610"
     CDS             complement(130273..130908)
                     /locus_tag="PK898_00610"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOU18563.1"
                     /translation="MGTDKLSLLFKRFYILIFAFAFSLYTGLAFLAPVLMHYDLILPG
                     KLIYKFFGFFCHQLPYRSFFLFGEQAFYPLASANMDHDIITFEEATQNAKMDFFDLKS
                     FVGNPQMGYKVALCQRDIAIYLAIVLFCFIFLISNYRIPRLHWILWIIIGLVPIGLDG
                     FSQLFAAINPAVFPARESTPILRVITGAMFGFNTAWFLFPYLDKKLKEDEA"
     gene            complement(130954..132069)
                     /locus_tag="PK898_00615"
     CDS             complement(130954..132069)
                     /locus_tag="PK898_00615"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOU18564.1"
                     /translation="MSKVMRENKINRRDFLKIATISGGALLIPKNVHSYFDFAAQLDT
                     FPQSEFIGRNCLSGIMNFRVKPEADAEINKSVYEDFLFPIYREVVGEAPAGTYNATWF
                     ETPYGYVYSPGVQLVKNEPNVPETALPDSSIGKGFWGVVTVPYVNLIFGKDPVSPWYI
                     SIKDHNPKLYYDQIFWIDEIKTASDGTILYRANELYGTYGDIVYADARAIRKITDEEV
                     APIHPEVENKRIYVNLTYQTLTCYENDVEVYFCRISSGAMYTNEGVLTDRYATPTGNH
                     HPWRKSISLHMSGSLTGSGWDTPGVPWNVIFATGGASIHGVFWHNAFGTPRSHGCVNA
                     KPDDAKWIWRWVKPEVGLDPGDITVSGDVGTLIEVKN"
     gene            complement(132062..132544)
                     /gene="smpB"
                     /locus_tag="PK898_00620"
     CDS             complement(132062..132544)
                     /gene="smpB"
                     /locus_tag="PK898_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOU18565.1"
                     /translation="MIVKILYAKIKPMGEKIVATNRKAKFEYFILETFEAGIVLKGTE
                     IKSVRAGQISLADAYVQINDGKEAWLMNAHIAPYDPANRYNHDPLRPRKLLLNKREIR
                     NIWNSIRLKGMTIIPTKVYLSHGLAKVEIGIAKGKKLYDKREAIAKRDADRERARRDE
                     "
     gene            132700..133965
                     /locus_tag="PK898_00625"
     CDS             132700..133965
                     /locus_tag="PK898_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU18566.1"
                     /translation="MRQLKYSFDTVFRTSLLFLIFLFLTGCNTPNSSSLQNSSSSQTP
                     QPDQTQIFANVLATANASIQQTILPENTEITTQVAELSETNIQDTGLPAPTSDPNRTP
                     PALPANFTTDLLNKLDTPHTYIADTCEFLKNRWSAGKAEPGTVVMVVMYHSIVQGDES
                     SVTLDNQISSEKNKQIAADLYNQGFTAIDTTQFINFMKYNDYIPPRSVLLIVDDRHHG
                     SYFADHFLPFYQQYGWKVINGWISSDDTTQELWDENAQVEAEGYVDHQAHGVVHNENM
                     TDASSDDFIYNELKGSIDRIQQHFGKIPKAIIWPGGNFGFRPIEIAKELGYEVGFTVS
                     PRGPVMYNWVPLCDETDPARPSYLADGLQDPLFVIPRYWDTDVSQHIDTVRQIGKAAK
                     EQILANRETELEYYDIVCKPKLGELGVQP"
     gene            133962..134318
                     /locus_tag="PK898_00630"
     CDS             133962..134318
                     /locus_tag="PK898_00630"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOU18567.1"
                     /translation="MRNFDSDCIFCKIAKGEISSEIVYQDEFVTAFKDLSPVAPVHIL
                     MVPNGHYLDLNDCAESDKELLGYILLVGAKIAKQLGIDQSGYRIIVNDGPDAGQTVFH
                     LHFHLLGGRPLAFKNQ"
     gene            134339..135952
                     /locus_tag="PK898_00635"
     CDS             134339..135952
                     /locus_tag="PK898_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU18568.1"
                     /translation="MKIFYGTSLLFFLFFFLLILPSGCMPEAPTSEITPLDLNGPIAS
                     LSAVVAMNLSETPEPTLIPTSTPKPKMNSSLALPTHPVFQLDAIPTLRLESVEYEIME
                     GDTISKIATTFQVSKKSLIEANNLINPDIISVGQKITIPPQTQDIRDKGFLILPDAEL
                     VYGPPSVSFQPEDFLSDHPKSYLSTYLEPTPTPLPKTQKDSSKESPIRFTPRTGMEIL
                     FQVSAENSINPKVLLALMEFQSASISNSKPNDHQKKSVIAYLGKSYESLSRQLNWAAD
                     TLNYGYYQWKNKRINQWILDDDTVVAVNNNINAGTAAIQYLFSQIYGKDDWVYAVSEF
                     GFAMTYRNLFGIDSQNYSWENTPESFPDLQLPFSAGEIWSFTSGPHYGWSSGTPWSAL
                     DFAPPDAIACSQSNLWVTASAPGKIVYSKGGLVIEDLDEDSNMQTGWSILYLHIEERD
                     RIEAGRTVESGDKIGHASCEGGIADGSHVHIARRYNGEWVPIDAKHPLILSGWEAFST
                     GSIYDGYLVKDDKIVEAWYYKTEESEISY"
     gene            135972..137768
                     /locus_tag="PK898_00640"
     CDS             135972..137768
                     /locus_tag="PK898_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU18569.1"
                     /translation="MIILVGFPSFACTRSVSKPAELTEAAIRFEITKIGMETSVPKNL
                     PLAEVQKTYQPIPYRSSTPVPTMPSYPIPTETAIKADNNAKQVATEKSHITPFRSIST
                     LMPTPRNTIPIEPINADVTFTPVPSVKAYVPVEIQPLIPTLEITIIPTEVVLPDLESI
                     NTEKLLYVTQNGDTLQNIANRFGVTSDQISSTQPISANGFLNPNINLFINAPKDRVYE
                     SAVKILPDEYIVFSKTATDYDLQNEINQAGGYLSYYSEETSDGLMSGSEIIYKVCRDY
                     SINPIVIIALLEYKSHWVYGFPRTPAEKDYPMGWIESTNKGLYKQVSWAATALSEGYY
                     GWRYGKISQIPFYKYPKPPYPIYFEPTLNAGSVAIQYLFSQLYVWDTVDAAIYSEEGF
                     FSTFTSMFGNVWENYSPDPNGLNANLRQIELTLPFSKTEKWAMTGGPHAAWTTGSPYG
                     ALDFAPPSQYHGCISSDRWVLAAAPGIVVRTGKGIVIVDMDGDGLEETGWSLMYLHIA
                     TNEKVPVGTVLERGSRIGHPSCEGGSATGTHVHLARKYNGEWINATGPLPFVLSNWTA
                     YEGSEEYLGGMTRGNEIVQASTVGSSESIIRY"
     gene            137819..139387
                     /locus_tag="PK898_00645"
     CDS             137819..139387
                     /locus_tag="PK898_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU18570.1"
                     /translation="MKVLFFRNKVISFSLLFVAAVVFLTACGTNKSATEQSQTTLTFP
                     LQLSPTPTELSSVSETLSGASKYYEKRPNYMPGEPVDYIVQPGDTVSALASRFNTTQS
                     EILKANPIIPAVTTTLPPGMPMKIPIYYRSQWGSPFRILPDSLFANGPAQIGFSARSF
                     VDRQPGWFKYFTAYTGEQNRRGGEIVDYIATTYSISPRLLLALLEYQTGALTQKHRPE
                     GINTNSVFGFTDVNSKQLTAQLNKLSNYLNNYYYEYREGRELEYQLLDGSLYRIDPWQ
                     NATTAALQHYFAMIDPPNEFFYAIGPNGFQATYIALFGEIEDHPDYIPGSLTQPKFTL
                     PFKEGDSWAFTGGPHPGWGDGAPNSAIDFAPPSSASGCVPSDEWVRVPAGGTIVRTDT
                     GVAVLDLDDDKDERTGWNIFFLHLLTSSIPPVGTRLNTGDAIGHPSCDGGTSTGTHFH
                     IARKFNGEWISAGGIIPFEFDDWKVENGTSAYSGKLKRYSSTVIACECADYTSIIHNG
                     PPLVPTPTSTVKPK"
     gene            complement(139401..140447)
                     /gene="tsaD"
                     /locus_tag="PK898_00650"
     CDS             complement(139401..140447)
                     /gene="tsaD"
                     /locus_tag="PK898_00650"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU18571.1"
                     /translation="MTNNEQIRILGIETSCDETSAAVVEDGCRIISNIVASQIDIHKK
                     YGGVFPEVASRQHVLSISPVIEEAMQKAHMSYDDLDAIAVTRGPGLAGSLVVGMNAAK
                     GLSLATGLPLIGVNHLEGHIYSAWLYGSEDKPPLTQPDFPAVALLVSGGHSEIILIQS
                     HLHYERLGGTLDDAAGEAFDKVARLIGLEYPGGPSIQKAAVFGDPNRFRFPKSKLEGT
                     WDFSFSGVKTAVLRKVRELQTEGMEELPVSDLAAGFQSAVTGILYEKTMDAARLYNAR
                     CIIIAGGVSANKALRELFENQSDFPLFIPKLSLCTDNAAMVGAAGYQRFIANKFDDLA
                     MDVFPTWPLSRVSL"
     gene            complement(140437..141717)
                     /locus_tag="PK898_00655"
     CDS             complement(140437..141717)
                     /locus_tag="PK898_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOU18572.1"
                     /translation="MKKDIQNLMKQKDVDAIIVSGGSEHNPPMTYLAKGEFFTKAIIV
                     LLKDREPVLFYRPMERDNAAKTGMKKICFDEFPTETYLREWDGDQAKADALELRDILN
                     RVGLRNGKIAFCGKTEFGTQYAMVQAFRELIPEFEILGSEGIDVINQARFTKDLEEIE
                     AIRKMGLTVTEVVRRVENFIKDDCRIKGNLLVGKDGEEVTIGDIKRKINLWLSELGGE
                     NPEGTIFSVGKDSAVPHNSGDDNQTIETGKTIVFDFFPCEMGGGYFYDFTRTWCIGKA
                     SDNLLNAYKQVKVVHDAIINSIEVGKPLKELQKQTCEMFEKMGHKTVLNSPHTLEGYV
                     HSISHGLGLNIHERPFSGFSAPDSDRAEIGTVFTVEPGLYYPEAKEPFGIRIEDTVFI
                     DHNGKANIIAPYPYEFVLDVAPYSDKNAEEGHDK"
     gene            complement(141743..142147)
                     /locus_tag="PK898_00660"
     CDS             complement(141743..142147)
                     /locus_tag="PK898_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18573.1"
                     /translation="MDKLRTYIFLGLFFSLPSGYGLYWLVMNTLPYLKERWLFFFLMI
                     IFITGIMMPIYAALNKYFFTIRKIITKTVVRESLVTGLLCDILLWFQIGRVLNSTIIF
                     LCVGGFILIEILLRTRDTVQYRSGFDDMKKDE"
     gene            142299..143066
                     /locus_tag="PK898_00665"
     CDS             142299..143066
                     /locus_tag="PK898_00665"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOU18574.1"
                     /translation="MLFTVDIGNTNITLGMFRGEDLIMRWRMATNRNSMPDEFGMQFW
                     FLFEHNGINPENIEGICISSVVPQITSKVEDACDYYFKRPILEVNYKLKLGMKIDIDH
                     PAELGNDRIADSVAVKALYGTPSCLIDFGTATTFNILNREGNYVGGAISAGIQTSADA
                     LFLKTAQLPNVNIQKPPCVIGKNTVNSMQSGLFYGYISMVEGMVERCKQELGDEDMQV
                     IATGGYAKILSQETSCIDKVDSWLTMTGLRLIWEMNQ"
     gene            143063..144280
                     /gene="coaBC"
                     /locus_tag="PK898_00670"
     CDS             143063..144280
                     /gene="coaBC"
                     /locus_tag="PK898_00670"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOU18575.1"
                     /translation="MNPLKDKKILLGITGSIAAYKAAEIASSLTKSGAIVNAVLTPGG
                     EKFITPLTLHSVTGRHASVDKDLWEGEQHVTHIELGHNSDLIVIAPASADFIAKITHG
                     FGDSLLALAVLAGHCPVLVAPAMDGDMYLNQATQENIAQLMKRDFQIIGPASGHLASG
                     LVGVGRMSDPQEILGAIRYTLSRGGILKNKKILVTAGGTQEAIDPVRIISNRSSGKQG
                     YAIAQAALDLGADVTLITTVTTLPIPYGCKVVYVKSAKEMQNAIFENLTDINALIMCA
                     AVADFRPEVSEKEKIKKDGSELLLRLEPTEDILLEVAKRRYEYTKLNAVIGFAAESEN
                     LIKNAENKLNKKKLDMIVANDISGKESGFESDKNQACFLFPDGSKRDLPLMGKDQLAE
                     LIMEEITKIIIKQ"
     gene            144366..145343
                     /locus_tag="PK898_00675"
     CDS             144366..145343
                     /locus_tag="PK898_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOU18576.1"
                     /translation="MKRKSILLSLIVIILICGCSNRKSPDSSSQSDRSISQSNNPYLA
                     DYQGESSNYNEAAYAPKMASLESQSNSEVTRLIVKTADMTIEVQDTNQALEKISNIAT
                     QFGGFVVQSNTWKESEYEEQSLLYGEITIRVKAENFDETIRMIENLTPNSEKNVLSKV
                     ISGNDITSDYIDTKSRLTSLENTRTKLNEIMATATTAEETLAVYKEIADIESQIEVLK
                     GQIKYMEESAALSSVTVKIHPIQPEKTIEVKEWSISEILKGAVQDLIDSAQDFGEWLI
                     YFVISVLPFLIIIFVPIYFIVRACSRRRERNKQKKADSKKTTDQKDNQP"
     gene            145436..147931
                     /locus_tag="PK898_00680"
     CDS             145436..147931
                     /locus_tag="PK898_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU18577.1"
                     /translation="MAARISEKEKEQLRHAGEIVYENITPEKDPVYTPIPSFVSDKVI
                     SLLSARGIRFLYQHQLLALEAEFNKNNVILSTGTASGKSLCYQIPIMEYLLRDPDSTM
                     LLIFPTKALTQDQRKNLASLIPEKIQEIAIYDGDTPPYQRAAIRRNARVILTNPDMLH
                     NGMLPFHPSWARFFENLKLVVLDEAHIYRGVFGAHTANVIRRLIRVHKHYTQSSLSPQ
                     FILCSATLSNAKQLAEKLVGSEVTEFSQDTSGNGVREIFFLNPPIVDEALHLRAGSIY
                     TAAKVAEIMEQKNRQVLLFCQSRQSVESTVRRLRDIGINADGYRSGYLSKERRKIENG
                     LKEGSNRCVVATNALELGMDIGGMDSILSIGYPGSIASYYQRMGRAGRNHQPSDFIMI
                     ASQNPIDQYLMKHPDYITEQKAEPALIDPDNLLILYQHLQCALSEVPFSFSESYGGLT
                     IDETQELLESFCSMGIARKSSGHFYWLESSSPQHTISLRNSGLDRISIVSQDFNGKKV
                     PVGDIDRPSAYWMVHEGAIYFHNGESYRIDELNLDENTAFARPIPAEYTTEADKKTTI
                     LTKNVLMKNSVQNADLSAAEVTVTTQVIGYKKRDIETNQILGSYPLDLPQEKLETKAF
                     ILNLSDQFREELRINSHWTNDPNQYGPNWFKIRKTILERDHFQCQLCSVSGTEQTLHV
                     HHIIPFRNFTNPVDANQPENLTTLCHTCHDRVEAAVKMRSGLTGFASAFRQISALFLE
                     CDANDLNVISDPACAEYDGKATIYLYETIPGGIGLSQNIFYNFIKIVSAVKDLIETCD
                     CEDGCPGCVGPAGEIGIGGKEEAVAIASGLLLL"
     gene            147953..149191
                     /locus_tag="PK898_00685"
     CDS             147953..149191
                     /locus_tag="PK898_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOU18578.1"
                     /translation="MGDILSQLRQLGVTLGMPENQDFVNGRHSSTLDALKITFPNGIV
                     GENEFGSYFINRKIYPMPMMHGCVELNDPLILTDNLLSILGIDSIDKKRVLAMDTETS
                     GLSVASSAFVFMIGMGYFFEDSYIIDQLILPDLGEEKAFLAQIENTFARFDTILTYNG
                     RSFDIPMISSRENFHYFPNSCKDVRHIDFLPLVRKYWGKSLENCRLSTLERDVLQVTR
                     GIEEVPGYLAPEYYREFLSSGDAEKIKGVAYHNQIDVLSLSTFLIYLSNLMQLNLNDT
                     EINDQCGISIQELRKNLLNHNPIDNPEIFCGNTFPVADRKKAAAYLQKHRETEKAIEI
                     YRSLVTEGDVDSCLALSSIYSKSTETTPQAIEYLKKGAEFVESDLCMGIWSKKEKQEK
                     IKTQIEKMERKLRKNHGREK"
     gene            149175..149486
                     /locus_tag="PK898_00690"
     CDS             149175..149486
                     /locus_tag="PK898_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOU18579.1"
                     /translation="MAGKNEIIYWAENEAEAVVIVAILEENGIPAIKLRESYGALNNL
                     SFGIFGEIAIAVDEDRAEEATAIINRQQESAANSDTNQENDENDASSDEIDKDDRDDP
                     F"
     gene            complement(149534..150391)
                     /locus_tag="PK898_00695"
     CDS             complement(149534..150391)
                     /locus_tag="PK898_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOU18580.1"
                     /translation="MDNFWKLHAVNDHYSITSLSEGKFVSINDSSRYTPDGAYTTFRT
                     YNRFFVLNLIKHFDRLEETARLAGKPVLLNRKEICKFLGEVIEKYPNNELRIRITVDL
                     SVCLGDIYIVLETLITPDIKCYENGIDVITTTLHRSNPKAKLNKFLSQANEVRRQKGF
                     EYEEILMVDSNNEILEGLSSNFYAVIRDEIFTAEEGVLFGTTRDYVLRLAKKLAIPVN
                     LRPVSIHELKYLDEAFITSTSRSILPIRSIDNQKIDEKAPGPITKKLMQAFTEDINSA
                     LEDIRTIGL"
     gene            complement(150432..151019)
                     /locus_tag="PK898_00700"
     CDS             complement(150432..151019)
                     /locus_tag="PK898_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU18581.1"
                     /translation="MKIGSIVLAAGNGSRFQAEQHKLLVRLNGKAVVRYSAEKALQAG
                     FLPVIVVLGYQKDDILVQLQGLPVLCIENPDWQSGQSTSLKKGIELIQNEVDAACLML
                     ADQPFVSIRTLRKLEEVQKKNPESIIVPTYQGKNGNPTMFPHWTFQGLIDQPANDLGG
                     RNIIKQYGAKYVEVKDPFVVRDIDTKEDLIDYSKK"
     gene            151112..151831
                     /locus_tag="PK898_00705"
     CDS             151112..151831
                     /locus_tag="PK898_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18582.1"
                     /translation="MGRKINLFKEIRPNPGEKILIYTAGICYILFISIDFIEFNPFNL
                     SAKLKYLSIWICFGITLFRFFSINRPGCSMEKKIGWINITAFFFTLSADYFLLFTEQY
                     GLGITLFCGVHIARLYLFSTLKKRTEIIFWEAFFASLIFFVCRSFIIAIASFYGFLIL
                     TVTIMSFRKLTFLADRYPVSKYLLRFGMILFCLCDICVAVYNFPGSSYFISRLSGFLI
                     WFFYLPAQLLLSFLIPPPLSK"
     gene            complement(151888..153246)
                     /gene="ssnA"
                     /locus_tag="PK898_00710"
     CDS             complement(151888..153246)
                     /gene="ssnA"
                     /locus_tag="PK898_00710"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOU18583.1"
                     /translation="MLIYNAKVVTWEKPNRILENSGVYIKDGVIQEVGSSDLLLKKYP
                     DEEKINADDQLLMPGSICAHTHFYGAYSRGLAIPGKPAKDFPEILDNLWYKLDRSLDE
                     ESTRLSAEVFIIDAIKSGCTTLFDHHASPEFIDGSLDVIADVVDKSGIRASLCYEVTD
                     RNGKDGTKAGIRENVRFLKRVKSGYTLDGRLSGTFGIHACLSVDEDTLSDCAAAIPEG
                     FGFHVHLAESQEDEWDSLYRFNQRCGKRLYDHHILGNKTIVAHAVHIDAAEMEMLKET
                     GTWISHQPRSNMNNAVGAAQVESMLANGMNVCLGNDGFTFDMWSEWKAAYFLHKVMNR
                     DPRRMNGYDVQEMAIYNNSRLAGQSFGYGEKFGKIVPGAPADIILVEYRPFDDLNAGN
                     IPWHIIFGFNERMITTTIVNGKILMKNRELTTMDEKEICARSLKKYPEVWKKFNAYHG
                     VK"
     gene            153787..153861
                     /locus_tag="PK898_00715"
     tRNA            153787..153861
                     /locus_tag="PK898_00715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153822..153824,aa:Pro,seq:cgg)
     gene            154010..155320
                     /locus_tag="PK898_00720"
     CDS             154010..155320
                     /locus_tag="PK898_00720"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOU18584.1"
                     /translation="MSKPTIISSPEILLPNKNVDLTKWATIACDQFTSNKAYWDEVES
                     IVGDSPSTLKITFPEYYLGKIDEAKKIQEIQDTMYRYLRDNLFVTHNGMILVERQIHN
                     GLRYGLLTTIDLEEYDYNRTSTSWIRATEGTVAERIPPRVRIREGAPLEVPHILVLID
                     DDQKRLIEPLVAQKESLPVLYDFDLMLNSGHVKGYSLADPELEQNIMDVIAKCQSDEE
                     ITRKYGPVSEKNRILFAIGDGNHSLATAKAIWEKHKSEWGMNHPARYALVEIENLHDP
                     VLEFEPIHRALFHETKSLISLLGDYFQDSMDVTEMKSMEQLFADIDHTPDGVQAFGVL
                     ESGKYYKVVLNRCSSALCVGSVQQFLDRCKSDHGFEEIDYIHGKEDLIHISSLEGNAG
                     IYLPPVQKNGFFQSIINDGALPRKTFSMGEAHEKRFYIECRRIL"
     gene            155427..156104
                     /locus_tag="PK898_00725"
     CDS             155427..156104
                     /locus_tag="PK898_00725"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU18585.1"
                     /translation="MKNRKKLALAGLLIIVLSILMVSCGSRPTPEPTVDVNVLATNAA
                     QTIEARFTETALAKPTDTPQPTYTPVSYTPTTSSAPITLPTAGTGGSVSIPTIAQQPT
                     AIVNLPTATLSAVGDKAVWADQSIKDGTHFAPGESADLTWYITNVGTTTWTTDYSVRF
                     FTGTNFAKAGNTRYYLPKPVAPQETGQVTIDIVAPTTPGEYKMAWVLSNAEDHNFYTV
                     DITIIVD"
     gene            156119..156697
                     /locus_tag="PK898_00730"
     CDS             156119..156697
                     /locus_tag="PK898_00730"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU18586.1"
                     /translation="MKTKILLIVTGLIFIVAGFSGISSQQAPSSDANALRTEVAGTVI
                     AEIYSTVQAEFSTIATQMAKTPEAQSGIIGEGFVKPTEVLYAHMAKLTKQDKNYLQYK
                     MYEDWDVTFTFKNVGLQDWTDEFYLRYYKGEVPIEGKIIYLPALPRGESTSVTLHFDG
                     KDEPGIYNSYWELINNDGAVILDNIFVAILVK"
     gene            156718..157650
                     /gene="queG"
                     /locus_tag="PK898_00735"
     CDS             156718..157650
                     /gene="queG"
                     /locus_tag="PK898_00735"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOU18587.1"
                     /translation="MDSSEIKEIIKAEAEKLGFCFSGVTSPDMPDDFFRYRLWLENRS
                     YGQMEYLNRKDAVEKRQYPQLLLNSVQSIVVLGTPIQPSPLTGSFRIAAFAHYKDYHR
                     QIYSLIEELIRRISEIAGEKPEYKICVDSSPVLERSLAVQAGLGWIGKSSMFISPEAG
                     SLVLLSECLLSWKLPYDNPYPNDRCGKCTRCVDACPTKCIDPQLRTIQADHCISYLTV
                     EHRGDIPAELAKKCGNQIFGCDICTTVCPWNQKTDTGYSPLERFHFSDNLSDDLNLTE
                     EEFKKKYKDTAIYRVKNEGWKRNIKIALQNLYED"
     gene            complement(157720..158424)
                     /locus_tag="PK898_00740"
     CDS             complement(157720..158424)
                     /locus_tag="PK898_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU18588.1"
                     /translation="MARKNSPQYVIHSYQKKRRVLPYVIWAIAAIILGLGILLLVLWI
                     KGDGNFSLGGLFASATPTSTETFTPSPTVPTSTPTITPTETVTPSPTISPTPEGPQMY
                     TVEEGDNCWDVAVNKFKVGIDVFLAINGFTMDNCNLTPGQEVIIPGPNTKLPTETPIA
                     LDQYTAGQQIEYKVQLNDSLRDIASKFNSTIESIIKLNKIEDENAPLTAGSTIIIAVN
                     IVTPTPTSVPTYTPVP"
     gene            complement(158524..161421)
                     /gene="uvrA"
                     /locus_tag="PK898_00745"
     CDS             complement(158524..161421)
                     /gene="uvrA"
                     /locus_tag="PK898_00745"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU18589.1"
                     /translation="MSNKNVIRVLGARENNLKNISVDIPRDKLVVITGLSGSGKSSLA
                     FDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAVSIDQKGTSKNPRSTVG
                     TITEVYDYLRLLYARIGIPHCPICGREVSKQSSDEIVEAIQKLPEGSRLMILAPIVRA
                     RKGTYQAVLEEIRKSGFVRVRIDGTVYSLDEEVQLDRYKIHTIEAVVDRVIIRHFEDP
                     EEEKMNRSRLADSVETALKVGEGYLTVTNLSSEPAQDMYFSEFLSCPEHGISIPEIEP
                     RSFSFNTPHGACPACQGLGMRLEIDPNLIIPDNSKSINEGAIAVLEWNNSRFDNGYYW
                     QQLLQVASAYDIDLDMPVSKIPKDKMKIILYGSNGASLNMVLTGRNDRKSTYRAPFEG
                     VIPNLERRYNETNSDYIRDRIAEFMKETPCQVCHGKRLKPESLAVTILGKNIIDVTEM
                     SINTTLQWAKDLYEKPGLLTDRQKIIAEKVLKEIISRLDFLVNVGLDYITLQRSAASL
                     SGGEAQRIRLATQIGSQLMGVLYVLDEPSIGLHAKDNAKLLQTLKKLRDLGNTVLVVE
                     HDSETIRSADWIVDMGPGAGDQGGQVVVSGPLNQVLQCRDSLTADYLTGRKFVPIPEQ
                     RRKPDERKLKIIGASENNLKNIDVEIPLGMFVCITGVSGSGKSTLLNDILYKALAHDL
                     NRAMTTPGKYKKIEGTQYLDKIINIDQSPIGRTPRSNPGTYTGVFDEIRSLFAELPES
                     KMRGYKAGRFSFNVHGGRCEACQGQGQLKIEMQFLPDVYVECDVCHGKRFNNETLQVK
                     FKDKSIADILDMTVDDGIEFFSAFPIIQSKLSLLQDVGLGYIRIGQPGNTLSGGEAQR
                     VKLSKELSKRMTGKTLYVLDEPSVGLHAADVHRLIDVLERLVEQGNSVIIIEHNMDII
                     KVADYIIDLGPEGGDRGGKVVTTGTPEEVAENKKSYTGQFLKKVLEQENPG"
     gene            complement(161439..162014)
                     /gene="ruvA"
                     /locus_tag="PK898_00750"
     CDS             complement(161439..162014)
                     /gene="ruvA"
                     /locus_tag="PK898_00750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOU18590.1"
                     /translation="MISYLEGTVIEKESDSITVMVNGIGLKVYVPQSLLGIAEKGKEI
                     LLHTQLIVREDLLALYGFETKEEVEYFSLLMGVSGIGPRLALGILSTVGVDTVRRAVI
                     QDQPDFLSRVPGVGKKTAQKVVLYLQGKISDSFENATLSNLHSADLEAIEALTALGYS
                     LVQAQTAVQSVPKDTPDTVEDKIRAALQSLG"
     gene            complement(162011..162832)
                     /locus_tag="PK898_00755"
     CDS             complement(162011..162832)
                     /locus_tag="PK898_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU18591.1"
                     /translation="MMITSTSNSKIKQIRQIQDKKQQKKSNLFYMEGLRIIGEAYKTE
                     NLLETVIFCPELLKSEYGNQLIEQGKQTGLEILEVSESVFKSFSIKENPQGIAAIGIK
                     KEYHLDDLNTKKGIIVALTEIADPGNLGTIIRTSDAVDANGIVLLGDSVNPYESGSVR
                     GSMGALFSQKIIQTDFETFLAWKNEKKIKMVGTSDHTETDYLEISYPNPIILLMGSER
                     QGLTREQIDSCDFMARIPMLRSSDSLNLAVATGIMLYQIYNCHRGYVLTSEKGNA"
     gene            complement(162832..163329)
                     /gene="ruvC"
                     /locus_tag="PK898_00760"
     CDS             complement(162832..163329)
                     /gene="ruvC"
                     /locus_tag="PK898_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU18592.1"
                     /translation="MVIIGVDPGTALTGYGFIDLSDRGELSVIDYGVICTPAKDKMEN
                     RLLQLYSEINEKILLYGPQRGAVEKLFFHSNVTTAISVGQARGVILLSLARAGIDVDE
                     FTPMEVKQAVVGYGGAEKRQVQMMVKAILKLNEMPKPDDAADALAIAICCANTNRFPN
                     ILESF"
     gene            complement(163337..164092)
                     /locus_tag="PK898_00765"
     CDS             complement(163337..164092)
                     /locus_tag="PK898_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU18593.1"
                     /translation="MSGHSHWATIKRKKGAGDAKRGQVFTKLAKEITMAAREGGDPNM
                     NFKLRLAVDKARESNMPKDSIERAIKRGTGESKDGAVFEDVMYEGYGPKGVAFMVECV
                     TENRNRTVADVRHALSRAGGNLGEAGSVAWQFSRKAYFLLTGDNLDFDKIFEIALENG
                     ADDVEQSEDGIEIYAEADAYKMMSDALFAAGLHIQEAQIRMIPNQPIDLPLDDTLQVL
                     RVIDALEELDDVQQVYHNMNISDEAIAALENEE"
     gene            complement(164279..165049)
                     /locus_tag="PK898_00770"
     CDS             complement(164279..165049)
                     /locus_tag="PK898_00770"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOU18594.1"
                     /translation="MKFIRYIQDNKPEVKIGWIYEDMVGGIEGDMFSDYRRLEATLPI
                     DRVKLLAPLIPGKIVAVGWNYQDHAKEFDRPVPEYPTIFLKPPSAVIGTGDGIVLPPT
                     SERVEHECELALVIGKKAKMVLPEEAFSYILGYTVANDVTARDLQKKDDTWTRSKGYD
                     TFCPLGPWIETDFEPADAMLTCRVNSELRQMASTRDMVFSIPAIIAYISSIMTLNPGD
                     VILTGTPAGTSPLKEGDILETQIEGIGRLVNPVVKQTK"
     gene            complement(165063..166001)
                     /locus_tag="PK898_00775"
     CDS             complement(165063..166001)
                     /locus_tag="PK898_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOU18595.1"
                     /translation="MLRYYKNNELILEKLDEPVTNCWIDAINPTPDEKQLLCNLGIPM
                     DFITYSLDIDERSRIEEEDNGTTLIVVRIPYFMGENVDIPYITVPLGIILTDKYIVTV
                     CEKSTETIEMFASGKAPKLSTTKRNRFALRILMRGTTNFLDDLRAINKTVDALEDKLY
                     ASMQNTELLEMLKYQKSYVYFAQALKQNELALYRISKLSSFKKYEEDEDLIEDVIIEN
                     TQAMDMTNISSSILSNLMDAFASVISNNLNVVMKLLASVTIILSIPNVISGFFGMNIN
                     FPDVIETHESTMIIIFGIIVLFTFFAIYIFQKKNWF"
     gene            166179..166579
                     /gene="ssrA"
                     /locus_tag="PK898_00780"
     tmRNA           166179..166579
                     /gene="ssrA"
                     /locus_tag="PK898_00780"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            167065..168219
                     /locus_tag="PK898_00785"
     CDS             167065..168219
                     /locus_tag="PK898_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU18596.1"
                     /translation="MSKKINLILTLLVIVGMLAAFIPASAQDSDVIKIGIVDSMTGGH
                     AAYGLASLDGYKLAHKYRDTALGKKIEFIIVDTKSDKAETALATSRAIADGAVALLGT
                     ASSGFTIAMNEVAAEKGIPTVTNFSTSPLVTQGNDIAFRSCFIDPYQGWALAKFAIEN
                     LGAKTSVLLVDISTDYPVGVANYFRETWNEMTGDSKTLLGYYSIMLGDRDFTAQLTAI
                     KQLNPDVVVVPDDYAEVGLILKQARELGMTMPFLGGDAVDLPEIEGIAGDAIKNDFYF
                     STHWHPDAFQNEASKAFGDYYRKEYNREPDAAAATAWDAYNMLIDAIERAGSTDPTAI
                     KEALYKTDMEGVTGHIKIDENGDAIKPVTILGYKDGVKKIIAIIQPEEKK"
     gene            168411..169304
                     /locus_tag="PK898_00790"
     CDS             168411..169304
                     /locus_tag="PK898_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU18597.1"
                     /translation="MTFDNFIQFVVMGLSLGAMYSLISIGYTIIYGILKLINLAHGDF
                     FMLAGFLGMWGALYYGVPLWLSLVFGLALTVGVILFVNWFIYRPLRQYKMAAFTSTFA
                     ISMIVQASVIMFLSARAQAFPHPDFLNTPIKIGNIIIPMVTPFIIVIALILFGILTLI
                     VNKTKIGRAMRAVSLDMEMVTLMGVNVENVIAFSFALSVVYAGMGAYLWGMRYPAFDP
                     NIGVVTGLKGFIGAVIGGIGSIPGAMLGGFLLGFAEIMLIGFFPQYTNFRDIFSYAIM
                     IIFLLFRPGGIFNVKIREEKV"
     gene            169306..170400
                     /locus_tag="PK898_00795"
     CDS             169306..170400
                     /locus_tag="PK898_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU18598.1"
                     /translation="MIKQKPKNTILSIIAIAILLVILELIGRSKIDHYYIRILQYWSI
                     YIIYGASFQLLYGYTGLLSLGHAGLIAIGAYAVGIFTISPATKTMSYILQPPAPFISN
                     VQIPFIPALIIGGVFAALFGLVVAAPALRLGGDYLAIVTMGFAEVIRLLIVNMPSIFN
                     GAMGLRAIPQSATLLMTWSVAIVCLFVMKRFENSTYGRALLAINEDEIGAEALGINVF
                     SNKVKAFVLSSFFAGIGGGLMASVLGTIDPNTFKSTLTYTAVTICVLGGIRSATGVII
                     ASGIYSIMSEFLRVFESPIKIGNFNFSGMPGMRVLVFGIMLLFLMLFRREGLLGSKEF
                     SWDWFFSLFKKKPPKEQEKTVHTDKMGGSL"
     gene            170397..171167
                     /locus_tag="PK898_00800"
     CDS             170397..171167
                     /locus_tag="PK898_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18599.1"
                     /translation="MTPILEAKNLTMRFGGLTSVSDFNLTMEKGELVGLIGPNGAGKS
                     TVFNMICGFYKPTIGEIIFDGKNITGMKPYRIANMGLIRVFQNGRLFKNMNVIENMMV
                     SKHMRFNSNSLKAILRTPSHNREEQRVFDESVALLERIGLKEMMYEPTGKLPFGIQRK
                     LEVARALCANPSLLLLDEPVAGLNVEETNDMMNFVDQLRNEFGITILLIEHTMRVVMS
                     LCPKIIVIDHGETIAVGIPDEIRNNKRVIEAYLGVDEQ"
     gene            171164..171874
                     /locus_tag="PK898_00805"
     CDS             171164..171874
                     /locus_tag="PK898_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18600.1"
                     /translation="MIQIEELNVRYGGIHALKGISFEVPDNSIVTLLGANGAGKTSTL
                     RSICGLVKPQSGHIYYGEDKKDLTKMEAAAIVRAGISMIPEGRRVFTGLTVEENLQMG
                     AWNRNDKDGIQEDIDVIFHLFPVLKDRFHQKALTLSGGEQQMLSVSRALMSRPKLLLM
                     DEPSLGLAPLLVSQLFKTILEIHKSQNMTILLVEQNMRAALKIADYGYVLETGEIVFQ
                     GTPEELSDNEKVQEAYIG"
     gene            171914..173044
                     /gene="hisC"
                     /locus_tag="PK898_00810"
     CDS             171914..173044
                     /gene="hisC"
                     /locus_tag="PK898_00810"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU18601.1"
                     /translation="MFDVHKAARSTIRAVHEYVPNKPPDSIMKEMGIEHFVDLATNEN
                     PYGISPLAYKAILRESKSSLNHYPESTCFELRKKLSEIHCLSPDCFIVDNGLDSIIRL
                     IGLTFLDAGDEIIIGSSTFSAYETTAKIMGAKVNKIPLTEDYRLNIDLMISACNRNTK
                     AIFICNPNNPTGTIITKSEFIKLMEKISPSILVISDEAFYEFSDSPDYPQTIPFLSEY
                     PNLIILRSFSKTMGLAGLRIGYAISSEEIIRMMMRVREPFPVNRLAQVAAMATLDDKD
                     FIQQTLEINRDSRVKMAKAMQLLRLKCIPGQTNFIFVDLGQPVEPIWNALFSKGIIVR
                     RLDGMNLPTGIRICLGKQKDLQFALNGLAEILGAPAPLIFDL"
     gene            complement(173161..174483)
                     /locus_tag="PK898_00815"
     CDS             complement(173161..174483)
                     /locus_tag="PK898_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU18602.1"
                     /translation="MFNKKNLFVIIVLLLLMTLYTPGFAAAGLDDIDWSVYHDVDFYG
                     QTLHALAENYPDIVKVYTIGHSWRERQLWTAEITGTGTEPKTGIYIVGNIHGGENEGA
                     ISAMYFAWWLVTNSEDAAVKDILDKYIIYVTPVMNPDGYAASAIYNTRQNLRPRDANG
                     DGKVFSDPYTDTNGDGFISEIYTGAQDSKVEDRVYVGMESPDWDKNGIPGDDPKNSGI
                     DLNRTFDYMWNYLDVDTDPILGEDIYKRAGPDAASEPEVQAVQNFMIYHPVYAVLSLH
                     TGEQSVLYPWCYTQEPTKDNDFFKGVAQKMIAAYEDVTGRPAYEKQSAHDYQTDSEMI
                     DWAYGRLGIHAYTMEVYAPGEPATEGDDSETHTWGNKLPEEKWEYVGDWQGLKDVWFK
                     TSPTAQMVRTAPPDQDKLVEGTKNAILELIKSEPNGDGPVVPEYLKWE"
     gene            complement(174698..177010)
                     /locus_tag="PK898_00820"
     CDS             complement(174698..177010)
                     /locus_tag="PK898_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOU18603.1"
                     /translation="MKHNLKLVGTAVPRHDARAKAKGEQLFSDDWTMPNMLYGKVLRS
                     KYPAAILRGIDTSKAEALPGVKAVLTAKDVPHNEDITKFGQMIDVGGGFEGLYKVLAT
                     GKIRSKSEAIALVAAETEEIAEEACNLIEVDYEPTEGVFDPREAIKDGAYLVSSEDNS
                     NVIMRTKIQDGDVDKVWDQCDIIIENDYYTSPNDHAYLETEGGMGWLDENGVITLRVG
                     SQVLEHYRTIAKILGVPHTMVRNISVPMGGGFGGKEDITVETYIALLVQKTRRPVKMV
                     WSREESIETHAKRHPEYLHYKTGATKDGKIIAQQVSIVLDGGSYTYLTPWVQMYSTYA
                     AAGPYKIPNVKIESVSAFTNNTLSSANRGFGSPQVNFAYERQMDQLADKLGISRVEIR
                     KRNIMHNGDAMPTGFIPQGHIALEELIDKVDKSLNATPKPDRLPDGRKVGRAIAIGHM
                     TYGRLTFMHDSSRVSIRIELDGTVTLRAGVPDIGGGQASVLAEIVAEELGLNIEDVHP
                     FIMDTHLTPLCGTTTATRQLYMSGNAAMKAADVMRDRILNEASKILKYPVESLALGDR
                     KVYLVNNPTVNVPFVTVVNSLSNNGGMLETIEQFNAPFTDVPNLKDIKGEVLPDLTYT
                     CHGVEVAVDEETGVFEILRIIAGIDAGKMVNKNSCEGQVEGGAVYHSLWATHEDLQWR
                     NGITKANNFSTYIIPTSVDIPDIETIILESGGGFGPYGAKGVGEPSDNSIAPAVINAI
                     RDAIGTDLNLNKMPITYEKIMCACKGIKID"
     gene            complement(177007..177495)
                     /locus_tag="PK898_00825"
     CDS             complement(177007..177495)
                     /locus_tag="PK898_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU18604.1"
                     /translation="MSNKIKINLKVNGENVTYEVEPQENLLRALRNNFNWDVKCGCEA
                     GECGTCSVLLDGKEVKSCLTLAAACDGHEIWTDKGLGLNDRLTARLQEAFVECGSVQC
                     GFCTPGMIMAGVEYLKNGGKADRKAIKYAISGHLCRCTGYIKIVDAIYKVAEEIEEGA
                     LA"
     gene            complement(177511..178374)
                     /locus_tag="PK898_00830"
     CDS             complement(177511..178374)
                     /locus_tag="PK898_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOU18605.1"
                     /translation="MSDFEFVNAPTVDSAIEALAQWGNKACLVAGGTNVMVDIRAKKI
                     KDGTLINIRDIESLKKITYTDGKVSIGALTIIEEIAKSEIIKQHAPCLFAAANVFADP
                     TTNRSATIGGNVGNASASGDTIPPLMVLNAIVHLKSKRGDREVPINEFIIKNGVTTRE
                     PDEMITHFTFESQPNTGFIKLGARRSMTISIATVAAYIETDKQSGIVKDCRIALGGVA
                     PKPVRGTNAEKVFIGNKLDEDTFTKAGEAVQNDISPRSPSVRATASYRRSVVPVLVKR
                     AVKLAAFGECE"
     gene            complement(178367..178852)
                     /locus_tag="PK898_00835"
     CDS             complement(178367..178852)
                     /locus_tag="PK898_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU18606.1"
                     /translation="MSLHEITLKVNGKTHKLVVKSNELLLNVLRDRLYLTGTKYGCGL
                     AECGACTILNGDRSIMSCLVPAVVADGWEITTSEGLADGDKLSPVQEEFINQGAIQCG
                     FCTPGMVVTSTALLRENPDPDEDFIRNYLRGNYCRCTGYAAMIKAVQVAAQRMKEEKN
                     E"
     gene            complement(178849..>179114)
                     /locus_tag="PK898_00840"
     CDS             complement(178849..>179114)
                     /locus_tag="PK898_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18607.1"
                     /translation="NNLVGSCRIAMGSVAGKPLFLKEIGDGLVGKTLTKELIIETAEK
                     ISKAIKPRDGGNRTTAAYRIETSKIISRDALEQAWERSGGKLS"
BASE COUNT        52804 a        36665 c        36089 g        53456 t
ORIGIN      
        1 cattccggaa tggattcgat gatgcatccc gatgctcgat ccgattttcc ggattacaat
       61 gcttttatct atagtatcct tcggctgccg gaatgtttga taaaagttca acgtattatt
      121 ctcggccaaa atattaaaat gtttgctgaa caaggctatt ccgatatcga aaaatgggaa
      181 gtagtcagtg cgagagcaag acgaagatat tgtctttata acaattccga tacattggct
      241 tgttttatca ccagcagatc tgatatcgat gatctaattc cggaaattac cgccttccaa
      301 attgaatgga ataagtttca ttatctttta atgaaggttc ccgatgaata cttgaaaaat
      361 attgatccta aaaacgaaac ggaatatcgt tttatagcgg aagcgattct atctccggtg
      421 gaagatctca atcggttgta tacaatttgg gggaaaaaga cagggacatg gctgcttgag
      481 atgaaaaggc gtgtttctga gctgagagtt aagttattgg acagttcctc tatccaatat
      541 tcgagatcag ctaatagttg gatgaagaat attttacaag aaatgcctga tatagttgat
      601 cggccgatat attttgtctc aagcaacacg catagtatta ccaatatgat ctcgggatat
      661 gctctctcca aagaatccga attaattgat tttctgaaaa aaatggacga ccagtccctt
      721 tttcatgaat gggatttaat cagccgaaat aaactttctg caaaaaaaga gaatcttctt
      781 tattatgttc taaaaaaata tcaacaaact cccgcgggag catacacagt caaagaacaa
      841 caggaaattg agaacgaagg ggggatattt cgcatatccg gatacaatac ttttgatatt
      901 gagacacaaa tcattgaatt atcgaaaata aaaacagaga atattgatcc gcgtattaaa
      961 ttggataata tcgaaaaatt gaaaaagagt gaagctattc tttttaatat tgactatccg
     1021 ttaggcatga ctgcttttaa tgttatgaca aagtttgcgg aagaatttga agatgtccgc
     1081 ggcatctaca ttatgggaaa agccgcatca ttaaatggga tctatgggga tgtcatcatt
     1141 cctaccgcgg ttcatgatat tcattcgcag aatacctatc tttttcagaa ttgcttcacg
     1201 gcttctgata ttgaaccttg gttggtctac ggatctatct tagacaatca acgggcggtt
     1261 tccgtattcg gaacttttct tcaaaaccat cgttttgtcg aaactttata tcagggcgga
     1321 tatacggata ttgagatgga agccggaccg tatctttccg ctatttatga aatggtacgg
     1381 ccggatcgtc acccgacaaa tgaatttata accttatata ataatcgttt tgatttagga
     1441 attttgcatt atgtttcaga tactccgatg agtaaaggaa aaaatctcgg cgccggtaca
     1501 ctatcctatt ttggaatgga ttcgacttat gcaacttcta tcgcgattct caggcgaata
     1561 tttactatcg aaagtcattt tataaaatag gatttttgaa ccaaaatctg aataaaggat
     1621 tcttttaatg agtcaaaaaa gtcgattcat cgttattatt ttgattgttc tccttatgct
     1681 tttgatcatt ttaatttatg gcgtttttat gaatgttcaa tccggtgtgg gctttctttt
     1741 tataccgtca aagtcttata cgccaaccat aaccgctaca caaaccatta ctccgactat
     1801 aacatttact cccagtaaaa cactcgattt gaatctgctg aaaacaaatg ctgtgaatac
     1861 atatgtcgct gttaatttga ttcaaactca aacccaatta agcatcgcca catcaaccgg
     1921 aacgcccgat gtttctgtta tcagaacgga agcggtaaac agagcgatca ccgaattgac
     1981 cgaaacggaa atggttgtaa gggaaacaga aaagtggatg ccccaaatca attcaaaagg
     2041 atttactgag aagattctcg gtaaagatca aagcataatg gttcttatcg atgatgtttc
     2101 tcggcctttt tggattgata aatatgaagt aaccaacgca tcttatcaga aatgtattaa
     2161 agacggtaac tgctcaatta tcgattccaa tgtgggagaa gaaatctctc aagatacgtt
     2221 taactcttta ccggtcgtca atgtatcttg gaatcacgca aaagattatt gcgaatgggc
     2281 agggaaaaga cttccgacta aagatgaatg gttggaagca gccggagtcg ataaggattt
     2341 cctttatccg tggggaaaac gagtaacgga agggatgccg gtaaacgcaa acgaagaaaa
     2401 cggaaagccg gtagctgcgg atacctatcc tgaggggtgc agcttttacg gtgtatgtaa
     2461 tttactggga aatgtatggg aatgggttgc ttcgtcaaat ccggagcctg attccgagca
     2521 gcaatcgatt atgggcggcg gatggcggac cttttctggc gatctgaata acaggattga
     2581 agggaaaatg aacaaaacag aatcagctga tgatgtcggt ttcagatgtg cgtcggatat
     2641 agaataagat tgaaaaattt tggcatgatt aaaaaaatag agaggttttg agaataatat
     2701 cccccaacct ctctatcgat tcgatttttt tattacggtt tcatatattt tatgatcggc
     2761 ctttgaatgg gaattcgatt aatctccgga attctattaa aaatagtatt tagttccctg
     2821 attctgaaag ccaattccgg agttaaatcc gatggtttaa attgataaca ccaatttcct
     2881 tcggctttac cggggaagtt cattcttgct tccggccctt gttgcaaaat atcttgtaaa
     2941 ggagcaatcg catataccgc gactgaactc catacgcagc gaaccatttt ccatgcgata
     3001 tcttctccgt tcgacataag gtatcttctt gcgaagtctt tttcggattc cggcgcggtt
     3061 tgataccaac cgattgcggt gtcgttatca tgtgtacccg tatatgcgat acagttttga
     3121 atataattgt gcggcaagaa aaggttcctg gcatcatctg agaaagcaaa ttgcagaatt
     3181 ttcattccgg gcaatccgaa tgaatcccgt aaatccacaa catcttgtgt aatttctccc
     3241 agatcctcag cgatgatcgg aagatcaccc aattcatcct tcaacttttt taatagagcc
     3301 gatcccgccc cttcaaccca ccgaccttta attgcggtta attctccgga tggcacttcc
     3361 caatatccgt tgaacccccg gaaatgatct aatcggatta aatccgcggt aagcagtgtt
     3421 gccttaatgc gccgaaccca ccaatcaaaa tcggtttttt catgttcggg ccatttatat
     3481 aacggatttc cccatagttg tccggtaggt gaaaaataat cgggtggaac tccggcaaca
     3541 acggtgggat gcccttctga atccaaatag aataaatcgg gattactcca tacatccgaa
     3601 ctgtccatgg atacaaaaat cggaatatct ccgacaattg tgattccttt tgaatttgca
     3661 tattgtttaa gttttaacca ttgtctgaag aataagaatt gtcgaaaagc gtgacgttcg
     3721 atcaactcgg catttttctt tgaaaaatcg ataagttccg tttgatctct ttttcgcagc
     3781 ctctccggcc aatcaagcca actatgccca tcttgtgtat ctttgattgc catgaataga
     3841 gaataatctg aaagccaata ttgatttttc tctttgaact cgataaattc cgattcaaga
     3901 ttaccaggat gatttttgta gttttcgtat acccgatcaa gaatcttcaa tttccattga
     3961 atcgcttttc cataatctat tcttgattcc gggaaaatgg gacgatccgt cagatcctca
     4021 tgagtcagat atccttcgtc cagtaatatt gtcgaactta caaggtaggg attcccggca
     4081 aaagcagaaa atgactgata aggagaatct ccgtatccgg tcggaccgag aggcaatatt
     4141 tgccaaaccg agcatttggc tgcggcgaga aaatcaaccc aatagaaagc ttgagggccg
     4201 agatcaccga ttccatccag gctcggaaaa ctggtggggt ggagcaagat tccggacgaa
     4261 cgttcaagat tcatgaaaag acctcactta taaccgaaat aattaactct ttgtatctat
     4321 agaaagcaac gaattataca gcgaaaaata ttcgagtgct tgtttgtccc aagaaaaatc
     4381 ctggttcatc gcacgaattt gtatttgttt ccagttttcc ctatcataaa aatactctat
     4441 cgcacgtctc atagcccatt cggtcccgcc tatatccgca ttttcgaata agaatcctgt
     4501 ataatcgttc gggaagtcaa acggatcttt tattgtatct tttaatccgc cggtagcgca
     4561 tgcgattgga atacagccgt ataacatact gatcatttgc gtgattccgc aaggttcata
     4621 tctggagggc attaataaca tatctcctcc cgcatacatt tttctggaga gcttttcatc
     4681 aaaacggata atcgctcgca ctctgtccgg aaattgattt tccaaatcga tacaagcgcg
     4741 ttgaacagct tcctcgccgg taccgaggaa aatagcttgc caattcaaat cattcatttg
     4801 attcaaagct tgaattgcca aatcaattcc tttttgaaaa gtcatacgtc caaccatgac
     4861 aaacaaggga atattttgat cttgcttcag gttaaaatct gcttgaaggt gctgcttatt
     4921 ggcgaaacga ttttcaaaat gattgacatc aaaattcata taaacggaat gatctgtagc
     4981 cggattccat tgatccatca ttatgccgtt caatattccg gataatttgt atttatgatt
     5041 tctcagaaat gtttgatatc cgcatccgaa agcttccgtc gtaatttctt cagcatacgt
     5101 ttttgaaaca gtactgatat gatcagccga atccaaaccg atagccattg gtaaattcct
     5161 caaataatcc ggaaaatcaa gatcaaatgc aggtcggata tcatacagat ccaaaatgtc
     5221 agaatgatct ccccctgaat aaggaagatt gtggatggtg taaagagatt ttgtccgaga
     5281 gaaaaaagga tcaaaactcc gattctgagc aagtagtttt acagtcaacg ccgtatgcca
     5341 atcgtgtgcg tgaataatat ccggtttcca attaattttt cgacataatt caaaagccgc
     5401 taccgaaaag aaagcatatt tttcaagatc tctatgggta ttgctgctgt aaacacccgt
     5461 attccggatc ggcatgccgt cgattaaata aatcggtacc tctgtattta atgcttttga
     5521 taagtaaaca tccttgaagc cccgtctgga ttgaatttta tagtttaaaa gccattcagt
     5581 cggccaattc ttctgtttaa tttgatcatg ataaggaata acaacgcgaa tatctatttc
     5641 ggttcggtca attttttctt tgggaagttt ttttaatgcg atcggtaaag ttccaccgac
     5701 atctcccaga ccgccgacct tgattaaagg atcagcttcc gaaatcagaa aaaggatatt
     5761 taatttcttt gccatgaatc attccagttc atcttttgtt tggaattaat ttattatcta
     5821 tattatagcc ttacgaatca tttcaaaggc agataaaacc gtttgatcaa ctgaaaatgt
     5881 cagtgaatta tagtttcttt ccgcatattc ttcttttcga tttgttcgaa gatatatttc
     5941 cgaacgaata aaagaatcag aaatcggaaa aatcgcacaa ataagtaacg gtgaatccga
     6001 atcaatctcc attttgcttt ttatattctg tacatgatct cgaaaagaaa aggacatatc
     6061 cggcatttct tgaacagagg aagtttgtat tgaacttggt gataaggcct tggtaattgc
     6121 gcctcccgtg ccatattcaa tgacattcag ttttacgtaa tttcgcgtaa tcatacgatt
     6181 tacgacttcg ggaagggttt catcatccac accgaaaatg ttatccccca aacggttacg
     6241 cacctcagtt tcaaaatcat ttaatagccg ttcggcttcg gctttcgttt ttcctttggc
     6301 tgttattctc agatcgataa ttccttgttt ggcggataag cctaaagtcg gattggaaga
     6361 tttttctaaa tctccgacaa attcgtccat aacggattca cccaagccaa aagtatggag
     6421 tgtcttaacg agaatactat cctgtgtcgg aaaatattta agcaacaaat ccgttccgct
     6481 atgctcgatc aggtacctca tttcggccgg aacacccgga agactgagaa caagttttga
     6541 gtctttataa acatagaaag ccggagcggt ccctaccgga ttgggtattg cgatcgctaa
     6601 ttcgggtata taagcttgac ggatattgtt ctcggaaaca gatctaccca ttttatcaaa
     6661 ataattgcgt atatccatcc ataattcttc tttaaattca gttttacaac caaaagccat
     6721 agcgacagcg tcccgagtag ggtcgtcgac cgtagggcca agcccgccgg ttgtaatcac
     6781 aatatccgac cgttctaatg actcttgaat agttgatgcg attcttttgg ggttatcacc
     6841 gatcagagtg gtgcgaaata catcaaaccc gagatcacgc agtttcctca acagataaaa
     6901 agtatttgta tcctgtgttt cacccaataa aagttcggac ccgatcgcaa ttatttctac
     6961 atttggcata ttgctccttt gaatcggagt ggtacttaaa taaattttta taaaaattat
     7021 cgctgtcgaa tagaagtcgc ttgattatta acagcggtct tattatagcc ttaccgacac
     7081 gaaacaggct tttatgactc atgattgatg attattttga agtagatttt cgagattcga
     7141 atactcttta caaggtataa tatttttatc cggaaggatt tttagatcat ggtttatctg
     7201 taaaaaattc gggagggaaa gatggagtcg aaaaaagcgg ttttcaaaac atcagccgga
     7261 ggcggtatta ccggaaccat tgaacaagtc tatgatgcca ttttaaaaca cggatgtaat
     7321 tttgagggca aagcttgtac attcggatcg gatccggtca aagcgaaagt cggattattt
     7381 aaatgggcag aaacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncattcg
     7501 gatcggatcc ggtcaaagcg aaagtcggat tatttaaatg ggcagaaacg gcagaagtca
     7561 acagcagcgt cgatgtcctc ggtgaaattg tctccagaat tgaataaaaa tttcttatcc
     7621 aaaccatatt tttcgaaata atataaatat cctttcactg tttttataac tgtgaaagga
     7681 tattttggat tttaaaacta ttttgataaa aatctaccga tgatcattct gaatggataa
     7741 taaaaagaaa atttataatg aaaataaaat atgatcaatt gattgatagg cttcagtaaa
     7801 taaaagcgcc cccagcagga ctcgaacctg cagccatcgg tttagaagac cggtgctcta
     7861 tccattgagc catgggggct tttcgtgggg atcaccttga acatgattcc gtgcggacaa
     7921 aattatacca tttatttgac ttctatcaat tcatattccg cgtgacccaa gccgattttc
     7981 tcagcatgtg tgatacattc tttccaattc gtatcgggat gaatgtcggt gaaatgatcc
     8041 ccggtatggt gggcttccgc caaccaactg cccgggttag gcggctgttg attcacggca
     8101 tcgatacatg cctgatcaag tgccactgga tcgaatgaag cgaacattcc gacatccgga
     8161 ataattgcca ggtcgttttc atcatgacaa tcacaattcg gtgagacatc tattacaaga
     8221 ctgatatgaa aggaaggccg atctttaaca accgcgtaag catattcggc tattttacga
     8281 tccagaataa tattcgattg tccccattcc ggttcgatgg catccacggg gcataccgca
     8341 atacagcgcc cgcatccgac acatttatca tgatcgattt gcgatttttt attggtgact
     8401 ttcggcgctc cccacgcaca aattttgaca cattttccgc agccgataca tttttcttga
     8461 tcgacaaacg gtttaccgct gctatgcatt tccatttttc cggctctgga accgcatccc
     8521 ataccgatat tttttaatgt accgccataa ccggcatttt catgcccttt aaaatgagtc
     8581 aaggaaataa aaatatcggc atccattaca gctgtaccga ttttcgcttc cttcacataa
     8641 tcgccgttaa tgggaaccaa tgtttcatct gttcctttta atccgtctcc gatcaaaata
     8701 tggcaaccgg tgctgaacgg tgaaaaacca ttttcatagg ctgaatcaat atggtcgaga
     8761 gcgtttttcc gccgcccgac ataaagtgta ttgcagtcga ctaaaaacgg cttccctccc
     8821 aggctcttca cgacatctgc tacggttttt gcataatttg gtcttaagta agagagattt
     8881 cccggttcgc cgaaatgcat tttgattgcc gcatatttat gatcgaaatc aatttgtccg
     8941 attccggcgg tacggatcaa tttttccaat ttgctctgac gattgttgac catcgtaatc
     9001 gtccgtaaat ttgtaaagta cacttttgat ttttccatag atttatatcc tcaactgtct
     9061 ttctaagttc ttccggatga ttaattcgga aggatattcg gagtatgttt aaaagttcgc
     9121 tcccaagttt ctttaagaag cgattgaaaa tcccgctgat cttttccgat aacaaccagt
     9181 tccggctcta attgagcgtg attctgcaat tttgtcaatt gaataaaatc ctctaccata
     9241 tcgatatgcc aataaccgtc cggagctttt aagaacccct tcaaacgaag gattttttcc
     9301 ttaatagtct caaggaaatc aatcaattca tcttgttcta ctgttttatc cgaagagatc
     9361 aaataattca caggacgatt ccaatcatga ttgctggtct cccctatgaa gtcgctcgct
     9421 attaaatgct cctttagaat ctctatcggg agaattccgt attgtgtatc gtacaaatac
     9481 gcttgagggt taattttctt taccgtttca tgaatcttct ccaaatgggc cggatcaaca
     9541 aggtccgttt tattaatcag aataaaattg ctggctgcaa tctgattttg aaccggagta
     9601 aaaatctcgt tgtattctaa aaaaaattga ctatcaacga tacaaaccgt accctttaag
     9661 gtatatttgc gttcggtata tggctcaatt tcggaaagaa ttttcagaaa gtttgaagga
     9721 tctcccaggc ccgaattttc aattaacagc aaatcaatcg gctttttgga gaaggaaatc
     9781 aatgtgtcaa cgaaattatt tttcaagcaa gcgcaaaaaa tagatccatt attaatttca
     9841 acgatttcag catcagatcc tttgaggatt tttccgtcaa tgccgaccgg accgaattcg
     9901 ttcactaaga taccgatttt tttatgatgc tgttgattga gaatatattg cagaaaagtt
     9961 gtctttcccg ctcccataaa accggaaacc agcaccaact gtatttcttc tttcgccata
    10021 tttatccttt gcgatttttc tttataagaa tccgatagat tgattataac caatcagaga
    10081 atgaacttta caatctcata ttatcgacta aaatctgacg aaaatcagga taagaagtca
    10141 attcgatata gtttattcgt ttgcataatt tatcacattc cgttcttaat gatttcgaaa
    10201 gcaataaatc gatggctccc gtacccgctg cgttcccgac ctggcggtat cgttcgatcg
    10261 ggatattcgg aaacatcccg atccggattg cgttctcaac agagaggtag gttccaaaag
    10321 cgccggctac aataaattct tctatatcgg acggttgaag atttgcttcc tttaaaagga
    10381 tgtcaattcc ggtccggata gcgctttttg caagaattat ttcgttaata tcaaaacgag
    10441 ttacggtaat atcccgatcg gttcccgaat cttctttgtt tacaagaaca aattcataat
    10501 tcttctcagc gggacggaca ttttttacct gccgattaaa gtttcctctt tcattaataa
    10561 cgccggatgc cctcaacgag ccgactatat ccaaaatccc tgacccgcaa ataccgattg
    10621 ctttttcctg atcaattgtc tggactttca tttcgccgtc gatcatctgt gctttctcga
    10681 tagcgccggt tgatgcccgc attccatttt gtatatgagc tccctcaaaa gcgggtccgg
    10741 aggcacaaga gcagctgtaa attttccctt tatgtgaaag gctgatttcg gtatttgttc
    10801 cgatgtccaa agccatctcg gtttttgata atttatgaat acctgtcgca aggagcatcg
    10861 cgatatgatc cgcgccgata tatccggcta cattattcgg taaataaaca taggcgccgg
    10921 gagcaacatt caaccccagt tttccggcgg gtacattgat cgaatctgta atcgccggaa
    10981 taaacggcat aacacccaac tgccgcacag gtagatttaa aaaaaagtga tggattgccg
    11041 tattacacac tgcgacaatt tcgacaattt gttcggactt ttctccgact tctgtacata
    11101 gctcgcgaat caagttgttt aatgccgtta tgagcttttc ttgtaaaatt tctcttccat
    11161 gaggatgtga atctgcatag gctatccgtg taacaacatc ttctccgtaa ctgatctgcg
    11221 gattggtgat tcctttttta gacagaacgg cggatatttc caagtcgacc aagtaagctg
    11281 ctatttttgt tgagccgata tcaatcgcaa gacccaaaat tcgtgatttc gaggggagaa
    11341 cagcaaccag ctcatcttct ctctgaacgg cacaaaattt ccaattgaat cttcggacgg
    11401 aatcggaaag cgattccaaa atattgtaat tacaacgggt acataggtga ccgttttcgg
    11461 aaagaccttc tttgatccga gttaaatccg agcgcgggtc cgataatgtc ggttgagaaa
    11521 gacataaatc ggtacgttga ataatacaat ccgacgaatc cgaaatattt ttgatccctt
    11581 ccagttgcaa ccgttgtgaa gtggaaagcg attcgggagg tacaaatatt tttagatcgc
    11641 ttttcgggaa ggctttacaa gctaaacggt aattttcctt gatttccttt tccgataaaa
    11701 acttcttctc ggtatctgaa agcggagtgg atgtccctga catcaatctt attttacagg
    11761 caccgcatgt accgtttcct ccgcataaag cggttatttg gattccggcc atttgcgcag
    11821 cttcgagaac agattgattt gaatttattc tgatccgttt cccgataggt tcaaaatcaa
    11881 tttgcaacaa atcgctctgt ctcaagagat cagctctttt ccggctttag aaaggtaaca
    11941 agtctgtttt ttatcgcaat aatcacactg tttggtggtt gtgtccactt cattgccgat
    12001 cccgatcaca aaagataccg atttcgccgg gatcattaaa aaagaatcgc ttaatcgaat
    12061 tcgatccgaa attttttcta atattgaaaa aatctgttcc tgtcccatgg caaccggcca
    12121 atctttcatg cccggattaa atgcagatgt tgcttttaag tcaggaggaa ttaaattctg
    12181 tttaaagtac ttttgagcaa gggaagatag tttatcgact gcggcaattc cggctgcgtc
    12241 caatgcaagt gcgtaagaag gatctttatg aaattccgcg gatactattt tttcaagctc
    12301 atctgaaatc gtgcaagctg ctatcaaaat ccgatttgcg caagccagtt gtttattaac
    12361 aatttttcct gaaatttgat gtccgttcgc cagaaaaagc cgctcatttt ctctctgaat
    12421 aacatctgat atttcataca ttacaaacgg atgcaacaat tcaccggcat ctttcagtgc
    12481 gttttccgca accgttatca agtgaggtga tctttctctg agaatattcg gattcgctcc
    12541 ttgagctctg agaatatcat caaccgaaat cgtaattttc caatttgtca ggatcggcat
    12601 attttataca acatctcttc ttttgaaaac atcttatgat tttatatact ttaaaatctc
    12661 attccgcaat tctgccgggt tcgggatcac agaagaatat tttaattcac cgttgataac
    12721 aatactggga agattcttaa ttcccatttt cataacccga gccacattct cttttttggt
    12781 aaatcgatat tctacaatat ccgcttttcc acccaattct tcaaccacac gcttcgctgt
    12841 attccacata taagtgcaag cagcgcaagt ttccgaatct aacgtaaaaa cttcaaccag
    12901 cggcttctcc aaatgaacat aatccggaag aataaccgaa tcaatatcat atgtggccgc
    12961 ttgataattt tcaagcattt ttctggtttc ttcaggttgt cgaaccgctt gaagaattcc
    13021 tacggtattt tcgataggcg tatcgtatgg catatcacaa cccggagaaa gaatccaatt
    13081 aaaatggtcg attgaatcaa tcaagtccaa tgcaaatttc atattatcct gttgtgtacc
    13141 cagaagcatg cgtgtagtca atgggatatt gccaccaata caaatattgt atcgatcggt
    13201 aatttctttt gctttaagga tgtccacatt ttcatcgata gaaatattat cgggtaaagt
    13261 ttggcacatg acttcaatag atttcgtggc atctccgcaa acaaagaaag aagacaagac
    13321 tcctttttca cgaatcactg taaaaagttc ggtataaggg tccgccaata tctgcttaaa
    13381 atggcgtgga gagatttggg aaaccagagg atcgacaaga gcaataacat ccatacccgc
    13441 atctacatat aaatcaatca tccgcataca gactcttttg caataatcaa ggagtgattg
    13501 cagaaattct ttttcctgaa atatatccat gaaaatttct gtccctctga ggtgagaagc
    13561 caacgtgaac ggtccgcaga tcaagccata tagtgcagtg tggtctccta cggcgatttt
    13621 catccgtcgc atgacattca aaatcatcgg aagtctgcca tcggtagctt caggaagctt
    13681 atcgggaatg gacttctcaa acgctaacgg gtgcgatgca atcgaaggtg gtgtcttttc
    13741 cgcccaaagt aaatcgcaac ctaatatttc cgcttcgatt tgcaaatcaa atacaatcgg
    13801 ctggccgtca ggatcataga ccttattaac ttctaacaag gattcaaaaa gtttttcttc
    13861 atcttttaat acttctaatg ccgaatatcc tttaagtttt cccgcatgaa ctccggcaaa
    13921 aggcacccat ggaacaccgt cgattctctc atgttttaag gttcgtaata ataattcttt
    13981 ccctttgatt tttttattca tttgacggtc cctcatacaa agttaatatt tgatttgtta
    14041 caccgattta acaccaaaaa aattataact caggaaaaga acaaatttca gtttaggaaa
    14101 aaaataagag gaaagcatct tgttttcctc ttattacaat tattcttcat taaaaagcga
    14161 aataatgaaa aggaagataa atttatagga tcggacgaat acccttatag acacgcggac
    14221 cgctcaaagt ttttagaatt gtctcagccg gacgatttat gagtttactg atttcttccg
    14281 aatctaacgg cgtatttcca ttcgaaagaa atattcgaaa taccgaatct gttaatgggc
    14341 cgtttcctga aataaaatcg tcatttcttg cacaatgatt tatgagaata tcttggagtt
    14401 gatcaacttg gatgatttca ccggtatcag aattaactga atctacaagt tgattatcat
    14461 tagaagattc aaaaaatttt ttatgcttat cgcagagata tcccatcagg gataaacgcc
    14521 aactgctgtc gttttccttc caccaatcaa aatcaatatg aaatttggta gaaaggactg
    14581 gttttatgaa cggtttagca aaaccgcctt cgttgatatt attcatattc tgtctctaat
    14641 ttggattctt tcgaattaat tttaaaataa taattatcaa gtatttcaaa atgatcatta
    14701 tgtatcagag tcgcgaggag cggctttgga gggcagcggt gcagcatatt tacggcagca
    14761 taaagctcaa ggaaatggac gataccctgt gggttatcac tcgatatttc cttgaagata
    14821 ttttcgacca atttcccgaa attaatatcc ggtttgttgt tttggtccca tatcaagtcc
    14881 aactggggat tattttccac ataaatacac attctttcat caaaatccgt attgatcggt
    14941 tgttgcctta tttcaaaatg gatttttcct tttgaatcaa acacagcggt tctgatccag
    15001 tctttcgatg atcgcggtgg aatcatcgag atttccacat cagtcgagtt atcaatcttc
    15061 cgcaatcgta taaaactgcc gggtattata tgattttccc gataccattg tttaagcccg
    15121 gaaacataat tctctttcac taatacccac cccgggaact ttgaagattt gttcttgtca
    15181 acgaattcaa aaatcacctt atcggtttct aaagcagtcg ggaaaatagg acgaatactt
    15241 ccgattagag gaagtgaacc ggctttccaa tgaggataat tgatacagat atcaaaagtt
    15301 ttgatttccg tttccggagt gatttgatta tcaggatcta attcatcatg aatttgtaaa
    15361 tgatctaaag aataaccgtt gatatccgct ttttcactgt caattccata atattttaaa
    15421 gtcagtggtt tttttctgac atcttcgggt tccagcaatt ttaaaaccca taaaactttt
    15481 cctgtcggac cgacctcatc aaagcgatca tctttataaa gggcataatt aaaagaaaat
    15541 tcggtgagca aatcattact gtccatcgga tattcgattg cttcgagaat ttttcttgtc
    15601 tgaacaggct gaccttcttc catttcaaga gcagcttcgg caagatttaa gtatccgaga
    15661 ttaacctccg atagaagcgc tttgggaaac caaaaatcag ctaaagtaaa taatccgtca
    15721 gattgagcga gcaactgccg gacttttttg gaaatcgacc cgccaaatcg atcgagaatt
    15781 tcttccgggt caagcaaaaa ttcggaatcc gaagaataat aatcgaatga attcaacctg
    15841 tggtcttgca gactaccggc aaattctttt tctgtcccat cctcaaaaag gaatgtgatt
    15901 gcattaaaat caggaagatc cggattatta ccttgccgga tattgacaac ttttgctttt
    15961 tgattatctg ataaaggaaa gagtatctcc tcgcccattt gataatcccg gttcggcctg
    16021 taaacctgcc cgcgagattt ttcttttttc tggagctctt ttctaaataa tcgtatatgt
    16081 tcgtcaatta tcgctttcga taattccaac gaagtcatcg gaacttcttt ttccagtaaa
    16141 taattataga tgaaatccaa atccgactgg ctgaggttca gatctttcca atttacaatt
    16201 gcatttacac ttttatcgtt cacaatatat tcccttccct aattcgtgat tcactattca
    16261 ttgattgtta gggaaaactg ttgttgatcc aagccagtgg attgacatag ataccgttta
    16321 cttttatttc ccaatgcagg tgaggaccga ctgatctgcc ggtcgaacct attaatccaa
    16381 ttaaatcacc tcgtttcact tgttgacccg cagtcacaaa ggtctctgac tgatgggaat
    16441 atgttgaata gactccccaa ccgtgatcaa taatggtgtg tttcccatga atcggtaggt
    16501 caccggaaaa caaaaccgtc ccatcagccg cggcataaat gttaatatta tttgccgtac
    16561 aaacaccaaa atctacccct gaatggaaat tcgtataaga accgtcatta taagtacggc
    16621 gatttccaaa gccggatact aagcagggtt catctacggg ataagacatc ctgtctcccc
    16681 aggattttat cggtgaggtt tgagttattc cggcaagagt cgcctgatca acttcatttg
    16741 aatgatcttc caatgtggaa gcatcgactc ccgagacaac ttcataagta aagactcccg
    16801 gttcgacaac aactttttgg cgaatgtcaa aggattcgcc gttcgaattc gaacctgaaa
    16861 gctgtaagtc ggtgagcccc gtttcctgtt cggcatcaac gccaaaaagt gcataccaat
    16921 catttgatgc ggtatcttcc tgataaaaat tcaggtcttg atttaggatt gcccccgata
    16981 atgtcaagga ataaggggtt ttgaggtgaa tcacttgtgt ttcgccttgc ataagcggaa
    17041 gatttgttat ttctatttga tcgattaatg gagaaatcgc atgcaccgga atattcaagt
    17101 cttcggaaac tgttgagaat aacaactgtt tttctgcgat atcccatgaa ccgtttaatc
    17161 gattgatctt ggctaatttc tccggatttg tcttctgcaa aacggctttt tcaaggcttg
    17221 tttcatcatt ctcaaaagtg tccacagccg agtatctgtt gccgttggta ttttgaacca
    17281 aaattaatgt ggaacccaca tagacttcag ataagcttga aatcttgttt attttgagaa
    17341 tatcgttgac atccatgccg gaaagaatgc tgatattggc cagggtgtct cctaaattaa
    17401 cactttgcat aaaaatggtt ccggatattc ctttaagtga cggaattttg attctatccc
    17461 ctatggctat tgaattagga tccgagattt gattgatccg aacaatttca tcaagagttg
    17521 tagaaaaacg agcggcgaca attgataaaa aatcgccttc ttgaattaca tattctggat
    17581 aaatatcgtt ggaattttgc cggtatgctg aagttgtcaa taccgcaagt aaaataagag
    17641 acgaaaaaat gattctgttt ttataaatca taaaactacg gtgaggattt taccataaaa
    17701 agaaaattac ttgtcaaata tcttctgata ggtatcatcc agttcatcat ttgcgaggtg
    17761 agtataacgc tgtgtcactg cgatattggt gtggcgggca aactcctgtg cgattttaag
    17821 gtttccggtt tcatgcagaa cggtagttac aaaataatga cgaaaagaat gcggtgtaat
    17881 cgttccgacc gcgtctttcc caaggcattc cgaaaccctt tccgaaacga tcaaacgtcc
    17941 ggtttttgtc gtaataggca aaatattttt accggaacct ttatcatgac gtgcaaagac
    18001 aggtaatgca gaaagcggga gcgtacctga tccgtcaaga acgcctcttt catttaaata
    18061 attgcgcata gacatgatag aacgcttaga aaatcgaatt acagcctgtt tattcccttt
    18121 tccgattata actgctttat ttcggaacca atcaatatcg cctctgcgaa ggccacaaat
    18181 ttcatgaatt cggagtcctg tatcggccaa tgtgagcaaa aaagctgaat ctcggagatt
    18241 aatcaatctt tgaacatcat tttccacagg tttatccggc aaagtttcgg cataggctaa
    18301 taccttttcg attgattcaa tgggaaattg cgggatgcga ataccgggtt ttctggaacg
    18361 ttgggcaatt aacaatttta cacgatgcat atttaaatcc gacaaatttt ccgctgctaa
    18421 aaattcaaga taatttttga acacatttat ataaagactt tcggtcgtcg gagaataact
    18481 ttttaaatac tttgaaaaat cggagacaaa ttcttctgta ataacggaaa ctgaactttc
    18541 tttatcaatc tgattttcat ccaacatttc aagaaaaaca tccaaaccgt tagaatacgt
    18601 tctggcagtg ttttcacttc ttgataagga aacgttatct ataaacattt ttatgctttg
    18661 aataatcgat acttgtgatg cgtccataaa accctttctt tcctttatgt gtatgataag
    18721 agtacttatc aaacccttag cattattata aacgaattgt atataatttg acaaggtctc
    18781 tacactctct taaaaatagt cacaaggatt tttcttactt tgattgcttt accgacaaaa
    18841 tcttctaatg gattcaagaa aagggaaaac atgatttaaa attcatgttc atatttcatt
    18901 tataataagc tcatgtttaa aacaaaaaag agatgaggtt ggagtaaaag aaatggaaaa
    18961 aaagccacgt gtttcaggaa gcacgatgat tgctcccgaa gtaattgaga ctatcataaa
    19021 aatgacagcg atggaaacgg acggtgtcag taaaattccc gcacaattaa ctcatcaacc
    19081 ggtaaggata cgtatcaatg aagaaaaaaa aattgatgcg gatgtttatg tgagaatcga
    19141 atatccttat aatgtcattc atgtcggcaa aaaattgcag gaaaatataa accgtgtgat
    19201 ttcagaaacg attggaatgg aaccgggctc aataaatatc catattgaag atatcgatta
    19261 tcttaacaac tcggaaaaag aaagatcctg actgaaaata ttatgtcgga aacaaacaga
    19321 gaattaaaac cagaagatca agataatcac aagacccttg ataacaaaga taatccagac
    19381 tcttcgcagg atgaaattat cgaaagtgtt gtaatcgata acattgagct ggatgaccgc
    19441 accagagccc gtggagttgc tttgcaagtt ttatatgaaa tggatatgtc cgatcatccg
    19501 ataaatttaa tccttcagga gagaattcaa tctgttccgt tagatgaaca acaaacaaag
    19561 ctggttttaa aaatcgttct cggtctccaa ccatatattc actatatcga cgattttatc
    19621 gaaaagtatg ccccggaatg gccgatcgat caaattgccg taattgatcg gaatattatt
    19681 cgaatcgctg tatgggaatt tgctatcggg gaaattacac cgattaaagt tgcgatcaat
    19741 gaatccgtcg aattagctaa gttattcggt tccgaaagct ctccgagatt tgtaaacggt
    19801 gtcttaggaa gcctggcgga acatatcaaa gatattcaaa aaataaaatc gtaaatattt
    19861 tcgtccactt tttttggaaa tcataaatca gatgtattta atttattcat aactgtcata
    19921 tttatttcgg gaaattccga gtataatctg atacattgga agttttttta tgaaagaaat
    19981 aaatacttcc cgtaatattt aacacaaatt tttgtaaaaa aattcaaaat ggaggaaagc
    20041 tttatggaaa atgtaggtat acccggaaaa tatgggctag aaaattacgg tattaagaat
    20101 gctggtaaag tttattggaa tgcggtacct gcgattctgg ttgaagaatc agttaaaaga
    20161 aatgaaggtg tgctttctcc ggatggcgct ttgatggtta acaccggtaa atataccggc
    20221 agatcaccta acgataaatt tattgttgac aatggtacag aggaagagaa acacattgat
    20281 tggggcaaag taaatgttaa aatgtccccc gagcatttca ataaaattta cgacaaggtg
    20341 ttagggtatg ttcaaaaccg agatttattt gtagctgatt tggttgcggg agcagatcct
    20401 gtctatcggt tgcccatgag aattatcact gaatttgcat ggtcaaactt atttataact
    20461 gatatgctga ttcgcccgtc attagaagaa cggatcggac aagttcccgg atttacaatt
    20521 atttgtgttc ccggcttgga agcgattccc gaagaagacg gcacgcattc aggagcattt
    20581 atcattgtga attttgcaaa gaaactggtt ctgatcggcg gctctcatta cggtggagaa
    20641 ataaagaaat ccgtattcac tgttatgaat tttattctcc cattaaaggg agtaatgacc
    20701 atgcactgct ccgctaatgt cgataagaaa accggtgatt cggctttgtt ctttggatta
    20761 tccggtaccg gaaaaaccac tttgtcttcc gatcccaatc gtttattaat cggagacgat
    20821 gaacatggct ggagtgacac aggtattttc aactttgaag gcggatgcta tgcaaagatg
    20881 atcaaattga gtaaggaaca tgaaccgctc atttggaatg cagttcatcg attcggtgcc
    20941 ttacttgaaa atgttgtata tgatccggca acgcgagtca ttgattttga cagcgacgcg
    21001 cgaaccgaaa atacccgggg cgcttatcct attcatttta ttgacgggat cgtagaaaac
    21061 ggactcggtc cgcacccgac gaatatcttt atgttgactg cggatgcaac cggcgtactt
    21121 cctcctttat cgaaacttga taacaaccag gcaatgtatt actttatgtc cggttacact
    21181 tcaaaattag ccggaaccga acgtggaatt accgaaccgc agccaaactt ctctgcatgc
    21241 ttcggttcac cgttccttcc tcttcatcct tcggtttatg cggaattact cggcaagaaa
    21301 attcatgaac atcatgcgaa tgtatggcta ctgaataccg gatggtccgg cggtccttat
    21361 ggaatcggga aacgtttcag cttaccttac actcgagcat ttgtgactgc ggcattgaac
    21421 ggtgaaatgg caaatgtaga atttgtcaaa gaaccgttct tcggattatt gattcctaag
    21481 acctgcccgg gtgttccttc ggacgtattg aatccgcgca atacttggga agataaggct
    21541 gcatatgatg ttgccgcaaa gaaacttgct gctgctttta ttgaaaattt caaaaaatac
    21601 gaagcaaaaa cagcaaaaga tattattgag gcaggcccga aggcttaact cggaatttgg
    21661 ttataaaaaa agcacgaatc gtatcgattc gtgctttttt tataaaatgt ccggactcaa
    21721 tttgtttggt atcatatgac gcttcccggt aattccttcg acgtctaatt ttaatatcac
    21781 cactctatcc aacaaaatcg gatcataatt tgcgaaagat aaaggcacct ttgaaaatcg
    21841 atttataaga attttcaggc cttcctgttt ttcaagattg tcggaaatta ttttgattct
    21901 gccaaatccc actacacttt cataaagtgt actcatttta caaggtatct ctgattctga
    21961 atttgtgact ccataagact tatccgcttc gaaaccgaca tgagcattct gtcgaatcaa
    22021 ttcgattttt cttccttgat ttgctgaatg gaaaaagaac gtaatcttgt tctcatgaga
    22081 taaaaaatag gcgaagttca gaggaataat atatggataa gggttatcga aaatagctat
    22141 gcgcataatc tcgatactgt ccaatatttt agcaatttct tcgacctcgc tgatttcccg
    22201 atctgctctt ctcataaatt cctcttctat aacagaatat aaaaaatcgc tcctgaaaca
    22261 aagaatggca ccaacggaat cgtcataaaa ttcggttttt tcagaatctt taaaattcct
    22321 gcggtgattc cggagagaat caatgcaaac gaaagcgcac ccaaaccatt gatgaatccc
    22381 agcgcaaaac cgaggacggt tccgaaaagt acatccccaa atccaaacgg gacgattttt
    22441 cctttatata tttttttgcc gataacaaac agaatatata agataagaaa gttgaacaac
    22501 gcccctaaga aattgttaat cccgattttt tgagtaatac cgggaaaaat cacaaacaga
    22561 acaaaaacga tcagcaataa aattggaatt ttatagaaaa ggatgtcgaa gtttgcgatt
    22621 aataccaaaa taatccaagc tgtttttgat agttttgcgg acggaagaaa attattttgt
    22681 atgagaaaac tatagataat ccatgaaaaa acaaaacaga ttataacgtc agttcttaag
    22741 aaaccggcta ttttcccttc attattcggt tcgtttggat aactttcatt tccgcttgtc
    22801 ggcaaaataa acttccgaaa acaatttgct gcccacaccg tggcaacgga gatgccgaag
    22861 atctgaagga ttgaatgaac ccacatatta aaataaaagt ggcgagggtg ggagttggac
    22921 ccacgacaaa gggcttatga gtcccctgct ctgccactga gctacctcgc cgtaagcgct
    22981 tgaaataata ctatagggag taaattttgt caaaccgttt ttctcgtaaa tcggtatcat
    23041 aaaataaatt aaatcaaata cgataagata gattcatatt taccgcttcc gataataata
    23101 tgatccagca tcgttatttc cagtaaattt cccgcctcct ggacgctttt tgtaacagca
    23161 atatcagcgg cgctcggttt cggatcaccg ctcggatgat tatggaccaa aagaatagcg
    23221 gcaacattcc tggtaatcgg ctttcggaag acttcactga tccggacagt cgagctattc
    23281 agagatcctt tgtacaatct gtcgattgca accaatcgat gccgtgtatc aacatttaag
    23341 acccataatt cttcatgatc aaatgctgac atctcatagc tgactaaatc aaagacatct
    23401 tgtgaagaat ttataatcag tctatcattt tcttcctgta atccggctaa tctttttccc
    23461 agttcaaccg cagctaaaac ctgagaggct ttcgcttcac cgattccttt tgcatttttt
    23521 aattcttcaa aagaaatttt ctttaatcca ccgagtccgc catatgaatt taatattcgt
    23581 ttagaaagat caataacatt ttctcctgcc gtaccggtcc gtaaaaggat cgcaagcagt
    23641 tcgtaattgg aaattgaacc cggaccgact tttaccaaac gttccctcgg tctttcattt
    23701 atttccatat cactgatacg attgtgatat acgtaatcaa tttcttttgc cattatagaa
    23761 gaccccgaat cgtagatgat gaaagtataa aagcactttt tattttacct tatcggaggc
    23821 atataatatt ataattatac taaatatata gtaattatac tttaaaaacg taaaaaatga
    23881 aaaccatgtc tttatgctat aattttgttt ttagaggata gttcatggat attgatccac
    23941 aaattttatc aaacattgtt caaattctga ctacgctggg ggttgcgttt cttacggcat
    24001 tatggttgag cattcttttt tgggtattcc gtgatattcg tcatcggaca agagatcctt
    24061 ttatgattat tttggcggta atcctggtaa ttattttacc gattgtcggt gtggttattt
    24121 attggattat tcgtccgaat caaacgatcg aagaaaaata ccaagctgct ttggaagaag
    24181 aggctcttct tcaggagttg gataaaaaac cgaaatgccc gggatgcggc agaaatgtgg
    24241 aaccggattg gattctttgt cccgcatgtc atacgcgttt aaataaactc tgcatttctt
    24301 gcggaaaagt tcttgaactt ccatggaatc tttgccctta ttgcggaaca ccccaacaaa
    24361 aaactttcag atccgaacaa aatcaataat ctgttattca atcgaaaaaa ggaaaacctg
    24421 agtgaaaatt tacgcaatga tcaatcaaaa aggaggggtt ggaaaaacca caactgcgat
    24481 taatctcggg tcctcccttg ccgctttagg aaaaaaagta ctggtgatcg atatcgaccc
    24541 gcaagcaaat gctacatctt ccttgggtat ggataagaat gaagttcgtt atgggactta
    24601 ccaggttttg atcggttctg ttcccataac ccctcatata atcgaaaatc gagattttca
    24661 cctttctatc cttcccgcct caccggcatt atcgggagcg gaaatagagt taatcgatct
    24721 tcccaatcgg gaaagcatgc tgaaaaatgc tttaaaaccg attcaagaga aatttgatta
    24781 tatattaatc gattgtcccc catcattgag cctattgacg atcaatgcgc tggtcgcatc
    24841 aaaagacggt gttattattc cggtacaatg cgaatacttg gcattggaag gattaggaca
    24901 actcacgaac acgatcaatc aggtacgtaa atcttttcct gaggttaatg tcagaggggt
    24961 tcttttgaca atgtatgatg gacggacaag gctcgctctg gatgtggtaa atgaagtaag
    25021 aaagttttat ccggataagg ttttcgaaag cattattccg agaagcatca gagtagcgga
    25081 agccccgtct tacgggcagc cgattaacta ttattcgaaa gacagctctg cgagtaaagc
    25141 atatgacgca ctggcaagag aaattttggc gcaggatcag aaataaaaat cagggaggga
    25201 atgaaaatgt gtgctcaacg aaaaggtggt ttagggcgcg gcttaggagc tttattacct
    25261 gtctctgata atgagatttc cgatgaatcg gtcagaatgg ttgatgtttc ggtaattcag
    25321 tcaaatcctc atcagccgcg ggtcgatttc aatgaagatg agttaaaaga actaaccgac
    25381 tctattttgg aacacggtgt aatacaaccg ttgattgtgg tgccagagga tgacggaaaa
    25441 tatacattaa ttgccggaga aagacgattg cgagccgcga ttgccgccgg attaagtatg
    25501 gtacctgtca tcacacgctc cgctaccgat caggatctgt tagaaatcgc tttaatagag
    25561 aatgttcagc gagaagacct atctccactt gaaacagcgg aagcatatcg tagcctcgaa
    25621 gtcaatttca atttaacaca tgacgagatt gcaaaaaaag tcggaaaaaa ccgtgtttcc
    25681 gttacgaata ccattcgttt gctaaaactc ccggaaaaag tcagaaatgc tctttccaat
    25741 cgaatgatca gtgaagggca tgcacgggct cttcttgccc ttaataccga agtacaacaa
    25801 ctatccgcct tacagactat tcttcttcat aatttgaatg tccgacagac tgaagaatta
    25861 gtgagaagga tgagcggaga aaagaccggt aaagaagata aaaagaaaga aaactcaccc
    25921 gaaatcaagt cgattgagga acagttgatg cagtcgatcg gaacgcgtgt ctcacttcac
    25981 cccggaaaaa aaggcggaac aatccatatt cattattatt cggatgagga actggaagat
    26041 ttagtggatc gttttaccgg aaggtgatcc atgacattga aaataacccg gatctcgcct
    26101 catcaagcga tcactctaag agaattaaat aaaaaagcaa tctattttca taaacacttt
    26161 gattggcttg agccggaaga tattgtttcc gatggtttat gttattcgct tctggaaaat
    26221 gatattccga ttgccggcag cggtttttct ccggagattg atcaaacgca ctggttacaa
    26281 tttttttccg tttgggaaga gggctacagt gttgccaagg tatggaaata cttttgggaa
    26341 aattgtataa agctgcaaat tttttccgag aaaacgatat tgtcaatccc ggctgatgat
    26401 ttgatgaatt tctttttaca acattccggt tttaaacaaa aaggaaatat tatctatttt
    26461 gaaggtgaaa aagagataat ttccgaccat cttctacaaa acaagcaaaa tgcaactctg
    26521 cttccattaa cagaatcgaa tgtaaaagaa attattgatt ccgtcgaagt ggcattttca
    26581 aaagtttggc gattatccga actcaatcta actcgggcga tacaagcttc gaatattgca
    26641 ttttttcttc gcgataaaaa ccaatccgtc ggatatcttc ttgctcttct tgaagaagat
    26701 tcaattcata tttcaaggat tgccgtcagt ccgaattatc aatcacgcgg atatgggaaa
    26761 gaattaatgt ggatcttgct gaaccggttt acaccgaaaa atgtcaatgc tttttcggta
    26821 aatacctatt caaatgacgg aaatgctgtt cgattttatc aaaatctcgg tttttaccga
    26881 acgaaccaaa tttacccggt atattatttg aaaattccta aaaattgaaa tgagttgtta
    26941 tccagaatag aaaaggcgtg ataaagagag caggaatcat gctggataca cgaatttttt
    27001 tgatatttaa taagctcgat attgcgattg ccccgagcat aacgccacct accgctgtca
    27061 ttccggcaat catggaatca ttcataatcg attgggcttg ttccgcaaga agtgttatcg
    27121 ttccttgata aatgaaaatc gggattaccg aaaaagcgac accgattccc atggttgtgg
    27181 caaatacaat agaggttacc ccgtctaata tggatttaat taataacaat tgataatcgc
    27241 ctgtcaaacc atcctggatg gatcccaaaa tactgaccgg tccgattgca aaaagcattg
    27301 tggcagtcag aaatgcctgt ataaattttt gtcgatcctt tccgtaatcc gatcgggtat
    27361 ttttatctac aaaagattga aaccaacctc ctaaattttc caatcctctt tcgatattcg
    27421 ttaattctcc gataattgtt ccgatgacta aaccgatcag gcacactaca ctgtcttcgg
    27481 atttcagaaa catgctgaga cctaaaaaaa gagtaaatat tcctatccca gaaataatgg
    27541 tatttctgat ttgttcatta atccgtttcc cgaaaaaaag accaaagaag gttccgacaa
    27601 gtattcctgc aacattaata attgttccga tcatagaatt attttccgaa tataagttta
    27661 tttaagaaat cgaatcgatc aaatgattgt ttttcttccc gaataaattg gatgttattc
    27721 ggaatttttc ccagtgaatt catccgaaag tgtcgttctt gaaaccagat accaggattc
    27781 tatattttgg aatcggtctg ctgtacgata tatgggaccg atactgactc ccttaacttg
    27841 tttgtctacc gcaaagttat aaaccgggtt gaacaaaggc agagccggta actcttccgc
    27901 aaaaaccacc tgaaagtttc gataaagccg ctctctctcg gcctgatctg tttctacgcg
    27961 agcggtttcc aaatattgac tgattgtccg attattccat tgggaataat tttgaccggt
    28021 cgatacttga gtctgatccc aaaaaggata aggatcagga tccggaaagt cggataaatt
    28081 cagatcaatc aaagcagctt cataatcccg ctcttccaat cgaacattga ttaattcgtc
    28141 atatggaaca gcttccagat tcaccaaaat attaattgcc gcccagtctt gttgaatact
    28201 ttcggcaatc tggcgatgtt tgtcatcgtc cgggtataac aacgtaaagt taagtgtttt
    28261 cccgtccttt gaacgaactt gatcctgctc gttcgcaaga atgtatccgg aagattttaa
    28321 cagctctttg gctttgtaag aatcgtattc gactttcggg atgttatcgt aatatgccca
    28381 gttaccgtac agtatcggtc cgtgagcaat gattgcctgt ccgtgaagga tgttattcac
    28441 gatttttgag cgattcaacc ccattaacat tgcctttcga acatttttat cctgaaaaaa
    28501 atcgacatta ccgttatcca aattcataaa aaccatagtc tgaatcggta atctggacgt
    28561 atatagcgct aaatccggtt ctgttaaagc ggattcgagt acatcctcgg aaatatagct
    28621 gattccgtca accaatcctt gctggtatgc ctgaaaagcc aaaatgctgt cttgatagta
    28681 attgatatgg atctcttcca agtatggctt ttttccataa tagagagcgt tatttttcaa
    28741 aataattccg gtaatattac ttccgttcag aatcagatca tctaaaatga aaggaccgct
    28801 cccgatcggc tgaatgttaa cttttgaatc taccatctgt tcaaaagtca tatttcccca
    28861 gatatgtttt ggcaggatgc cgacagataa ataatccaaa aacggagcat atgccgatgc
    28921 gaggacaaat tgaactgttt gggaatcgat tgctttaatt tgaattttgt tccaaaaatc
    28981 gattaaatca tccggaatat atccgttccc cttttttaac atctcatagg taaaaactac
    29041 atcatcagcg gtaacgggtt ggccgtcgtg ccattttgca tttggattca atgcgaaatt
    29101 ataggaagtg ccatccattg aaattgccca gcctgctgca agatccgaaa cgggtaatcc
    29161 tctgttatca aattttatca acccgctata aactaatcgg cagacgtctt ggtccgcatt
    29221 attgtaaaaa gacagaaggg gattcattcg ctgaatattt cccaccaagc cttctcggta
    29281 aataccaccc atcgtcggtt gcgattcaaa catctgaatc ggtccgtttg atgtgtcact
    29341 gagaagtaaa atgcctacta ccaatccggt caggaaaata attaccaact gccatctgat
    29401 ttttttcata agactatttt acctgtatcg gctatgaatc tcattacttt ttatcttttg
    29461 attaatattt tgtgagtaaa agtctaaaaa tcgaaaaaaa gggtgtctct cgatatctga
    29521 gacacccttg aacctgagta aattagaaag caagaattct gacgagaacg aaaattatta
    29581 aaagggcgct aaatccgatg gtcatgtaaa aaagtacttt ttcaatccct cttcgggcag
    29641 aaaaaacggc accgggttct ccacccgcaa tacttcccaa accgctgccg cggttctgta
    29701 atacgacaca aatgaccgtc gctatcgaag ttataatgag cgcaatatct aaaaagtttt
    29761 tcattcatcc cctcccgcaa aatcttaacg gtatgattat atctatatta catgaaaacg
    29821 gcaagaagaa tgagagaggt tgtgttattg tccggcgcaa atggaagaaa tcacagtaaa
    29881 cttacataat cattcggtta tttcagatgg tacgggaaat tactacgaga tcgcacaagc
    29941 aggtttaaac gcaaaaatcg atgtccttat cattacagat cataatgtag tcgttcgggg
    30001 taaagattcc tattattacc gggagggaaa taaattatta ttgttaatcg gtgaggaaat
    30061 aaatgatcct atcggagtcc ccggtaatca cctgattatt ttaaacagtt cacgagagta
    30121 tgcatccgct gcatccgatc cgcaaacagt catcgattcg gtcaataaag aaggtgggat
    30181 aacaatactg gctcatcctt tcgaaaatgc aaaaaagcag ttgcatatga aaagttttcc
    30241 ttggtctgac tggagcgtac atgatttttc gggtttggaa atatacaact ttatgagccg
    30301 gttcaaatgc ggtgtcacaa atttcttttc attaccgggc cactacttac ggccgattaa
    30361 ttttcagatg ggagcagatg aaaacacact tgcaaaatgg gatgaattat tatccatggg
    30421 atacagagtc agcggattcg caggatcgga ttctcatcgc tttcaaaaaa aagtcggtcc
    30481 gaaaactttt aagatctttg actatgaatt gacctgtcgt gcattaaata accatgttta
    30541 tattcccaaa ccattagtcg gtgatgttga agaagacaaa aaagttattt atgcagcctt
    30601 acgaaatggc tgtttttttg taggggcgga tatgatcact tccactgcag gcttcagttt
    30661 tcgagccgat acagaaaccg gcatatatta tccgggagca cgaattccga ttgggaatag
    30721 tgctacatta aagattcgaa ttccggaaaa atctatctgc cgattaatta gcaacggaaa
    30781 agtgtataaa caatgggaaa gtgttcaatc ggttccgata acaattacag aacccggcta
    30841 ttatcgagtc gaatgttatt taccctataa aaacaagttg agaggatgga tttattccaa
    30901 tccgatttat tttttcaaag gataattttt tcgatgacta ctacgaacaa tgttattcga
    30961 ttcctttctt caaaaaaaat cgattttcag gtttttgaat ccgaaccggt tcggcgaagc
    31021 gcgatggaga cagccgctct ctatcatttg gatcctatga ttgtatataa aacaatcgtc
    31081 gcatttaatc cgatccggaa aaaatatttg ctttgtatca ttccggcacc ctatgaagta
    31141 aatttaaaaa aattagcgtc ggcgattggt gaaaaaaaag cggccatcgt cacgcaacat
    31201 gaagctgaag ttttaaccgg tcttgaagcc ggagggattt ctccttttgc gttgtatcat
    31261 aaagggtttc aaatttgggt tcatgaaact atcagggatc aggaaaaaat ctcagtttcc
    31321 ggaggccggc gcggattatc cgtccttcta tcaacgggtg atttcattca gattttgcag
    31381 ccgaaaattg ccgatatagc cgatattcaa caggacatac aaagttccga atgataaaaa
    31441 caacccgata tacattcgtc attttaatcg tcctgattgt gttggctgcg gtttggtttc
    31501 aattcatacc cggtgatact tctcatcatc tttttttgat cggttacgct ttctgccacc
    31561 agaacccgga gcgctcacca tttgccggta caaatcaatt tactttttgt tatcgatgca
    31621 ccggcttgta tgtcggtatt ttgctggcag cactttggtc ctttcctctt cgtaaatcga
    31681 taaatttatt ttcgaaaccc ctctgcattt atgtgggttt gagccttctt ttttacctta
    31741 cagacagttt gaatgcgtcc tttttacttc cgttattcgg attgcgaccg atatataaag
    31801 atcaagaaat gattcgattt ttaagcggat tttttatggg gacagcttgt tccgtaatga
    31861 ttctttcaat attttcacgg cttttctacc ttcatcccgc tgtcgataaa aaaaccgaaa
    31921 aaaggaattt gcaattgttc ggatttctta tttccggttt ttttgtgact tatatccttt
    31981 ttaagggtga aaatatattc tttaaaaaga ttttgaactt tctgtgttca ataacagcac
    32041 tttccttcct gtctgttctt tattcgattt tgatattgat tttctcaaag accgaaaacg
    32101 agtatcgttc gatttgggaa gggaaaaata ttcttttaac cgggttattt ttctgttttg
    32161 tacaagtcgg tttgttaact ctcgtaagat tccgtctcac agatacatgg agttggtttg
    32221 ttaaatcaaa agtgccaata gatcttccgg cgaataaaaa agttctattt ctgtccccgc
    32281 ttggaatttt ttttcattaa taacggaaaa aacggttccg ggagctattt caacatgggt
    32341 ttcgtaattc gagcccttaa atatactttc tttgatgatc cctttgatcg aaaaatccgc
    32401 atcatttgta agttgaagtt tttcatctcg aaacagaatc catatttttt gtccttccac
    32461 caaaggatat tccagcggaa tggtcaaata aattgttcga tcattaagtt ttaatagaat
    32521 ttgagagtca gtgacgccgg ttgcaggcaa caaattattt aagcctaaaa atcttgccac
    32581 ccataccgat tttggtttta agaatatgtt ttcgggtgta tctgtttgaa caattacacc
    32641 gtcatgcaac aaggcaatac gatcggcgat actgaaagct tcgtcttgat catgagtgac
    32701 ataaactgac ggaattccgt tttcccccag tattctacgg agctcggcta ataaagaaac
    32761 acgcaaagta tgatccagtg cccccaacgg ttcatctaac attaaaagat gtggttgagg
    32821 agccaacgct cttgctaaag ccactctttg ttgctcacct ccggataatt ctgtgacctt
    32881 ccgatttgca aaactttcca tcccgacttg ctttaacaat ttttcaacta tgggagggat
    32941 cttttcggaa ttaaccttat tcatttctaa tccgaaagca atattttcaa aaacagtcaa
    33001 atgaggaaat agtgcataat cttgaaacat taatccaaaa tttcgtttga atgggggaac
    33061 atttaaaatg ctgacgccgt tccaaaagat atccccgtga tcgggtttct ccaaaccggc
    33121 aataattcgt aagagcgtgc tttttccgct gccgctcttt cctaaaaggc atagaatttc
    33181 atgatccgct aaatggaggt tcagattttc taagagaggc gtcttttcat attggtacga
    33241 aatatcttta agctgtaaca tcagttatta aatctcactt cccgattctt ttcggctgaa
    33301 aaatcattgc ttttgaaaag aattactttc aaggataaac gtgcccacta aacaaaaggt
    33361 catcagaatt gttgaaattg ccattgcttg accatagttt gaagcaccgg gttggccaag
    33421 aaaacgatag attgcaatcg gaatggtagg tcggtccggc atggatagaa aagatgtggc
    33481 tccgaattct cccaaagata tggtgaaaga aaaaatcccg gcattaatca tcgcccgcct
    33541 taagatgggg atatcgattt tcaaaaatac tttccaagga ttcgctccca atacggtcgc
    33601 cgcatgatgc agagagatcg gaatattttg aagaacaggt tgcaaagaac gaataacaaa
    33661 aggcaatccg attaaactgt gtgccagagg aattaataga ggattattga tttcatgact
    33721 gaaataaagc agataaccta atcccagcgt aactgcggag gtaccgatcg gaaataagaa
    33781 aatcatgttt attttctttg tccaaggata tctgtccccg gcaaaggtga ccatcaaacc
    33841 gatcatcaaa gaaataaaag aagctatggt tgcattcaac aaagaatttc cgaccgccaa
    33901 aataggagga acataaaaaa aggaatttcg ttcattaaca aaaagttttt tataatactc
    33961 cgttgtccaa gcgaccgact gagaatcata aacaacaaaa gaacgaataa ctagacccag
    34021 cagcggtgaa accagaaaaa cagcaaccat aaaaagaatc aaaatgatga tgattttttc
    34081 ggatggttta gtcggttttc tcatattttc ttcggaaaca tggggcatac ggttggactg
    34141 aatgttctta ccgattttat tctcgataga agttacggcg accgtaacaa taatttgcag
    34201 aattgaaagc agaccggcaa ccggaagatt gaataattga agagcttgtt gtgctatttc
    34261 gacttcaatc gtcctgaatt tggccccccc caacaaaagc actacaccgt agctggtgaa
    34321 atcaaacaaa aaaacgagca acattgcaga gaaaattgcc ggttttaata gaggaaaagt
    34381 aattttccaa aatgtttgcc atggtgtgca tcccaaggtg ctcgccgcat tttccaattt
    34441 gatatcgata ttttcccagg catttccgac aatccgcatt acaacggata tattgtaaaa
    34501 aatatgcgcg attaatataa tccagattgt gtttattaat tgaatgggcg gttgagaaaa
    34561 tccatgtttt tgcatcaaat aaacattcaa taaacctttc ggaccgatca atgcattaaa
    34621 tgccgcagcg gttactacgg taggcaagat aaaagggacg gaaaataagg atttgagaaa
    34681 ttttttaccg ataaatttgt aacgtgaaaa gatccatgca accggcatgc cgagtatcat
    34741 ggtcagaatc atggataaaa cagcttgcca agttgtgaaa ccgaagactt ttccgatttt
    34801 gtcccaatta atatgaaagt cttctaagaa ttgagtaatg actgtttgaa aaattgtaca
    34861 taagggaagt aaatagaata aaagaaagaa tccgaccgga ataaggagca gcaaactgcc
    34921 ttttataatt gaagtaacgg atgatgtttt tctttgagcg gtcactgttt tgataacccc
    34981 gtctctgatt atagcatttc atccggatga gaaaaattct gtttagatcg tattgaattg
    35041 acgaaaacat cgatttatgg ttataaatgg ggtatgaaaa aaagaaatct gttttgggaa
    35101 gttttcttgt ttatcggtat cagtgtctta ttgttaggat gtgatacaaa gcgaaatgaa
    35161 ttaattcggg ggttctttta tacggaaacc cccattcctc ctacaccaac cgttacgatt
    35221 actccgactt tatctcccac gtcgatcccc actccgttac cggaatatcg aattgaccaa
    35281 gccgataaat atctgttagt cggagattat gataacgcga taagcgaata tgaaaaagtt
    35341 ctttcctcca atcctgagga accttattat gccgcttcgg ttttagggaa agctcgttct
    35401 tattatttca agcgtgacta tgagagctgc caaaatacgt tgatcgatgg cttgaatacg
    35461 attaaagaaa attccaacaa acctgatttg tggtttcaaa tggcggattg tgttcacgaa
    35521 ttggacttat atgaggaaga aatcaatgca ttgaatgagt ttctgaaatt acgtcctgat
    35581 acacaaatgc gatccgaaat atatgagcgg atcggtgaca gttattattc tctcaaatct
    35641 tataaggaat ctcaaaatgc ttacctatcg gcagttgaag gcaccgatat ggaagaatcg
    35701 agctggatat ggatgaaaat tgccgatacc tattccgttc aggaagatta tacaaatgcg
    35761 ttgaagacgt ggcttgacct ttatgatctt tcgaccgatg acactcaaaa agcaagaatc
    35821 gattcacttt tagcggatgt gtacctgaaa ctggattccc cggaacaagc atatgcccga
    35881 tatcaagatg ccgtaaataa ttttccgcga acatatgaca gttatttagc tctgttgact
    35941 ttattaaatt cagatcagac tgtcaatgaa tatcagcgtg gtttgattaa ttactatcaa
    36001 ggtcaatatg ccctttcatc cgaagctttt catcgttata tagaaagcaa cccggctcac
    36061 aatggatcag cttactatta tatcggtctc aatcaaatgt ttctgggtga ttatcagagc
    36121 gcagtcgaaa catttgctac attgattgaa aactatcccg ataaccaatt ttatgcttca
    36181 gcttgggatg agaaatcata tgttgaatgg tattatctgg agaattttgc gcagggtgct
    36241 caaacgctaa tcgattttgt taagttacat ccggattatc ctgaagcacc gggattcatc
    36301 tatgaagcgg gaagaatttt tgaacgagga gatcgtttaa ctcaggctgc gacacaatgg
    36361 gagcgattaa tcgatgaata tcccttatat gaaaacagta tcgatgcatt gtttttatcc
    36421 ggaatttgtc ggtatcgtac aaaaaactat gacgctgccc tttccacatt taatcgtttg
    36481 cttttggtca gcacacaacc ggcggacatc gcgagggcac atttctggat cgcaaaagtt
    36541 tatgataaaa aaggagaaag tgggagcgcg aagcaaaatt acgaatcggc cgctcaggat
    36601 tcgcccaccg attattatac cgaacgtgca aaggaaattc ttgcggggaa aaaaccacta
    36661 tctttttcgg agaattacaa ccttacgaca aatctgtccc aagcccaggt gatcgcggaa
    36721 caatggatgc gattaacatt ttcgattccg gacgaagtgc cgctggatca accggatgcg
    36781 ttattattgg attcggatta tcggcatgcc gatgagcttt gggaattggg acattatcaa
    36841 gatgcaatcg cgatgtttga tgcggtacga ctcaaatatg aaaacgatcc ggtaaactct
    36901 ttcagactat taaacaggct tgtttcactc ggagcgtggc gaccggcggt ttacacaagt
    36961 cgacagattc ttacatgcgc gggtctgatc gaagatgcgc gaacattgga agccccgaac
    37021 tacttcaatt ggattcgtta cgggacatgg tttaatgaaa tcgtcctaaa agtttttgat
    37081 caatataaaa ttcatccgtt cattctctac ggtttgatgc gacaggaaag tatgtatgat
    37141 ccgtggattt cttcttcagc cggagcatcc ggtctgatgc aaattatgcc ggcaaccggt
    37201 cttgaattat ctaaaaaatt acaatggccg ccaaattacg ccgacagcga tttattacgg
    37261 gcaacggtcg cgattaattt ttccgccgat tacctttcga cacaacacga ctatttcgga
    37321 aatgataatt tctatatgct tgcttcttat aatgccggcc ccggaagcac ctccggttgg
    37381 gtagataaag ccgagaatga tcctgattta ttcttagaga ttattcgttt tgaagaaacg
    37441 cgtacctata tccgaaatgt ttacgaatat gcaaaaatgt atgaaagatt ctacggcgta
    37501 gattgatagc tcaggaagat tcgatcaaat ttcgagtaaa tttaatagca ccttcgcgat
    37561 ccgttatcaa attttcaatt tgagcttcta aaatcgcatt tttcagctcc ccgatttcag
    37621 gaccggccgg aatattaaat agtttactta tctgatcacc tgaaagaagc ggctttggtt
    37681 taatgatcgt atcaaagtga ttaaaccaag catcaaaata tcttgcagca tggtccaaca
    37741 ctttcgtcca ttgagcggaa tcccttaata tatctttatt aatatgtgtt tcaattaatt
    37801 tcagaaaaat accgtcgatt ccgtaatcgc cgaatttttg gaaatatcga tatataacta
    37861 catccgaata ttcctctaaa ttgaacagag agtgaagttc cataaatgct tcataggatt
    37921 tgataatgag cttcatttcc gctgtactga gtaacatttt ctttgaccgt gattttaata
    37981 tctcacttac ggatcgagaa ttctcctccg ttcgataaaa tctcgcaatg attgagaata
    38041 tcagaagcga tcttctttca tgaattagat tattatcatg atagaaaaac tcattgagct
    38101 gatcacgaaa ggaacctaac tttaatacag ccattccgga aataagattt gatgattcgt
    38161 cttctctaaa ttcccgagct aatatattta ttaaatgttc ggctgaacgt agccaatcat
    38221 atagcgcttc atcatttgtc ggatttttac ccggaaaaag gaagtcgagg aatgcatatt
    38281 tatcaagcaa tcggacagcg gtaaccgttt tttctccatc taagattttg aacagctcat
    38341 cacgatatct ttcgacggat gcgacagaca ggaaaggccg gatttcatta agtgcatgga
    38401 ttaagtcgcc atccattgtc aaatcataat ccactgccat gcggatcgcc ctcattcctc
    38461 tgagcggatc actttttatg ctttccagat tgcacatgcg cagcttcttc tctttcagat
    38521 cggatacacc gcccatggga tcgataaatt tattcttttg aatgaaatcg atcgcaatcg
    38581 catttattgt aaaatcacgt gaatataaat cttcttcaag agttgaacca tttaattgag
    38641 aaatatcaat acagtaatat tcatctgaat ccgcgttcaa caaaactcga accatatttc
    38701 tgtcgtcatc taatacatag aacttgccgt ttaactcatt ggcgagattc cttccgaccg
    38761 gacgaaccaa tccttcgata acaaaatcaa aatctcttac ttttttgccc agaataatat
    38821 ctctgacagc gccgccgacc aaatatattt gcacactgga agggactact tttttaatag
    38881 actcaaaaat ttcgctatag tcgtattgct tcgccacgtt ttattttttc ccttttattt
    38941 tttctaactt ttccagatca actacaccga taaacggcag atttctaaaa taatcatcca
    39001 aatctaaccc atatccgtaa acaaatttat ccggtatgac gaaaccgcaa taacgaatag
    39061 gtatttcaac ttctcttcga tcttccttgt caagtaacgt gcaaatacaa agcgatttag
    39121 gatttctcgt tgataaaact tgtataacag cattcaatgt atttccgctg tctacaatat
    39181 cttctacaat gataacattt ctgccctcaa tatttgtatt gaggtcataa gtaattcgca
    39241 cttctccgtt tgactttctt gtgccgacac cgtaagaaga aactgccata aattcaattt
    39301 gattgggaac ggttacatgg cgcatcaaat caatcagaaa tacgacacct ccccttaata
    39361 tgcaaataaa tacaatatct tcgttttgat aatcccggga aatttcttca ccgagctctt
    39421 ttactctttt ttgtaacgtc gtttcatcga tcagaacttc cgccaggaca tcttgatatg
    39481 aatacatatt attcctcccc aattattttc ccgggacaat atgatgtttc ttacatcggg
    39541 cttcatacat ctccgatgca ccgacaataa ttacaggatc gttataattt gccggttcgc
    39601 catttatgat acgttgcgtc ctggtagccg gttccccgca taccatacaa attgcgtaaa
    39661 gcttatccac cttttccgct tttgccatca atacaggcat cattccaaac ggctccccac
    39721 gaaaatccat gtctaatcct gctaaaatca cccgtattcc ctgatttgcc aaatcatcga
    39781 cgatattcac aatttctttc ccgaagaatt gggcttcatc gattccgacg acactggtct
    39841 ccgggtcgag atacttataa agatcggtaa tctcggctaa cgggatagca tctatttcga
    39901 tacctgcatg ggaatgaacc cgttctatga tatagcgatc atcgataatc ggtttaaata
    39961 cttggacttt attctttgcg ataattgccc ttcgcaaacg ccgtatgagt tcttccgttt
    40021 ttccgcaaaa catcgacccg catatcactt cgatggcagc aggtgctttc ttcaccatga
    40081 gtatctcctg agatattaga ctttgttctg attttctata tcgcttattg tactcccatt
    40141 tctctcttgc tcatttatct acaataaccg gagtatggaa atcggctgtc ggaaagcctg
    40201 ttaggaggca ttaaggaatt tctcaaatcg aaatcaaatt tttggcaaag attgataaat
    40261 catgcgaatg gcttcgggaa ttagggaaag taagtcgtcc gcggacatgg aataaggctg
    40321 accgatttgt cgaaaagcaa tttgagctgc ctttgcatga acccacaccc cgagacaaga
    40381 cgcctcaaac ggaagtgatc tttgagctaa gaatcctccg atcaaccccg ataggatatc
    40441 tccgctgcct gctttcgcta agattgaatt tgcatatggc attacagcgg tatagccgtc
    40501 cggcgaagca atcacagtga atgcaccttt tagaacaata acggttttcc attctttgga
    40561 tttttgttcg gcaatttgaa ttctgttcgt cttaatttca tcgacggtca tattacataa
    40621 tcgagccatt tcaccttcgt gaggagtaat aacaaaacaa tcgggaatat tcttttgcca
    40681 atcggggata tggggaagag cattcaaccc gtcagcatcc attaataacg gtaccggatt
    40741 ttctttccat gaaatcagaa tacgatgaac aagatcgaat accttaggag attgtcccaa
    40801 cccgggaccg atgaccaacg agcctttttt cttcgacata tcacggattg aatgcaaagc
    40861 gatttcgatc ccttcatcgg agttaaaatc agccggttgc cagatcgcct gagaaaaaat
    40921 accggcattc agatttctga taatctccgg ggcaaccata ttaacatacc cgcagccggc
    40981 tttgaatgca gcgattccgg agagtttggg agctccgata tatttttctg atccgccgac
    41041 aattgtcacg gtaccgaatg tgcttttatt tccgtcctcg ggtctgatcg gcagaaacat
    41101 acggacgact tccgagtcca ataccgtgcg atgaattgtt tgataaaccg ctagatcctg
    41161 aggtaaatca agggatattg tttcgagctc ccccaccagc agattcgccg gcggtaaaac
    41221 ctcacctatt ttaacggctt cgatacagat cgtgcgattc gcacgaatac aatcgggatc
    41281 aatttttccg gtatcgcaat ccatgccgga tggacaatca attgcataaa ttagagatgg
    41341 attcttacgt tgatttattt ttcgaaaaat gggacgaaag gcttctccca aaggaagatg
    41401 aaagccaatt ccgtaaattc cgtctaagat aatcgaactt tgctttaacc agttatcaag
    41461 ttgactctct tccgtatcgt tttcataatc aacggctttt cccgatacat taagaaaatc
    41521 tttgagataa cgatcttccg attctctttt tgagagcagc caagcaaagg tttcccaacc
    41581 tttttgagcc aaatatgtta atatcgccaa agtgtcgcta ccgttgtttc ctttcccgat
    41641 cagccccaaa accatcttgt ttttatcgga acaatcggaa tctaaaatat tcgcaatttt
    41701 taatccgacg gtttggatca tatgatcaga cgatataccg gcaccaaacg cttggttttc
    41761 aatttggcgc atttctgaaa ctgatactaa tctcatggca tcctcgcaaa atcgatttct
    41821 ataaaatgac cggtccgatt cttaattgaa cgtgtcttta ttctaattcg ttaaaaggta
    41881 aaatacagga atgaagcgtt ttattttctt ttccgtcttt atcgccggct tttcttctat
    41941 ggctattgaa atgacagctt cccgaatgat cggaaatatt ttcagttccg caaatctaat
    42001 ttgggcatgc gtgatcggct caattatgat ttatatggca attgggaatt ggttgggagg
    42061 tattttttca gacaaatatc cggatctttt tcactatttg atattaattt ttatttcagg
    42121 gattttttgt tgcctaatcc cgctcttttc aagacctctc ttattaaaag gggctcaagc
    42181 gatggatcag atgacacttc gttgggtcct tattcttttt ctaattcttc ttctgatcct
    42241 ttttatcccg atgacattat taggaatgat catccctttt gcgattaaat tgttgaatac
    42301 ttactatccg ttccgtggta aagtaaacgg ccaattactc gctgtctcta cagtggggtc
    42361 gttcatcgga acgatgcttc cgtcgtttat tttgattcca tctgtcggaa ccaacagaac
    42421 tttctttgtc atcggctttt tgttattgct agccggagcg atcggtcaat taacgaatat
    42481 aggatccgat aaaaaagcgg aaaaacttat gatcttttgt atttttttgc cgattttatt
    42541 attttttctg tttcctttaa aaataaaaca aacaaaaggc caaatttatg aaaaggaatc
    42601 agtctataat tatattgagg ttgttgaaaa ttccggatat accatattga gattaaatga
    42661 cggacaagga atccaaagca tttatcatcc ggtgatcgat aattattacg gaccatggga
    42721 acaaattttg accgcgccct tttttcaacc cgctccgatc gatccgggaa atattcgaag
    42781 agctgcgata ctgggaaatg ccggcggtac ttccgctaag cagcttctga aagtctttcc
    42841 taatgtaatt gtggatggat ttgaaattga tcccgaaata accaaagtcg cgaaagaatt
    42901 ttttgatcta ccttcggaac gaatgaatat tatttcaacg gacggacgaa tcggactgca
    42961 aaatagtgat gatcagtatg atattatcat ggtggatgct tatcaatctc catatatttc
    43021 agtcaatatg tgtacacagg aattttttca aattgttttc gaaaagctca atccggaggg
    43081 cgttttagta atgaatatcg gaaggtctga cgatgatcga actttgctcg gggctatcag
    43141 cgaaacggtt aaccgaatat tcccgcaaat atttatagta gatattccga attcatacaa
    43201 ttctgttcta tttgcaatga aaaaaggcgg ctcgattcag aatttgcaaa ataattatga
    43261 tttattatcc ggtttgaaaa atgtgcctgc tctactaaca gaaacattat cctttgcgat
    43321 tgggaatatc ggagaattgc ctgaaaaaag aggattggtt ttcacagatg ataaagcgcc
    43381 cgttgaggca atcaccaatc aaacaattct ccggacattt tttaaacgaa atgaataaaa
    43441 aaagagatta tcgaccttta tgaaaatcat aataaaaagc tttcatgttt tcctttccat
    43501 taccttaccg tttgtaatat taatgttctc tgttgttatt ctttttaagc cttggttcct
    43561 ggtatttgaa tatcgaaaaa cgaatttccc gccggatcct tatggttttt caatcgaaga
    43621 acgattagac tatggaattc aatcggttca atatatcgtt aataatcgat ccgatgattt
    43681 tctgacgaaa ataacattca agaacagtaa cactccaatt tataagtcgc aagaaatttc
    43741 tcatatgtcc gatgtcaaaa atgtctatcg tacagccaga ttgatttggg aagcgttgtt
    43801 aattctttac ttgatcctat ttctggtatc ttggaaacat cctgcgcaat tattgtcttt
    43861 tttaaaacat tttattatcg ggaacttgtt ttcgatctta ttcttagttg cggtacttct
    43921 tttagtagtt ttggcatttg atcctttgtt tgaagctttt cattctctgt ttttcaaaga
    43981 aggaagctgg ctcttttacc aagatgatac attaattcgt ttattccccg aaaaattgtg
    44041 ggttgatgca tttctaattg ccggaggact ttcatttatc ttctccgttg cttttttctt
    44101 tataggaaga aatgcttata ggcggatatc aataaatagc agaaacggac gtccgctata
    44161 aatctataaa gaacagcacc gaaggtttta tctttcggtg ctgttcttta ttgtattttt
    44221 ttatttattc aagactgatt gcactgacag ggcagttatc agcggcttcc ttagcgcttg
    44281 attcaaattc cgaaggaatt tcttcttcta tagcctccgc aactccttct gcagtcatat
    44341 gaaatacttc agggcaggta tcagaacaga gaccgcagcc gatacagact gattgatcaa
    44401 cttttgcttt cattttattc tcctttgttt tttggtggaa aaactacgat taagaacatt
    44461 ttaaatcgct ttaaaccgta aaccgcatgc ggatgtccgg caggcattaa aatggaatct
    44521 cctgttttta gatcaaattt ctcaccgtca attgtaattt gaccctgacc gtctaacact
    44581 aacaccagcg catccccgct tgattcatgg gtactgattt cttctccctc atcaaaggcg
    44641 aatagcgtta aactgaccga ttcattttgt gcaagcgttt tacttacaat ttgaccgggt
    44701 aaataactga cttgatcagc gaacttgaaa acctgtgaga attcaagatt tttaataaat
    44761 tggttcatgt ttcctctttt attcttcgat aatatttccg tcggttgaaa taacgataac
    44821 ctgccatgga atcatttttc cgcaacttga tagtgcctga attgccgcat tttcgatccc
    44881 tttacaacac ggtacttcca tgcgaacaat tttcacactt tttatcgaat tatttcgtaa
    44941 aatatctgtt aattttttac tataatcttc ttgatctaat ttcggacagc cgatcagagt
    45001 cacatgtttc ttaatgaaat cccgatgaaa gttattgtag gcaaaagcgg tacaatcagc
    45061 cgctatcaat aaattacagt tatcaaaaaa aggcgcattt acgggaacca atttcaactg
    45121 gaccggccat tgcattaatt cactggtttg tgattcccga cttgactcca tgatcggatt
    45181 ggatgtatct cgtcgcaatg tctttgcttg ctttcccgga caaccgcttg gcacattcgt
    45241 catattgttc actgtcgctt gatgcctcac cgcttcttta tcaaaaggaa tcgcttctct
    45301 ttcaatgatt gtaatcgcat ttgccggaca aaccggcagg caatttccga gcccgtcaca
    45361 ataatcatca cgaattaatt ttgcttttcc gttgaccatt tgaatggcat tttcccggca
    45421 agcttcggcg cataacccgc atcctgtgca tttttcttca tcgatttcca ccatttttcg
    45481 tatcatgtat ttgcctcatt atggctgttt tattttctga tcatattgta atatactgaa
    45541 tcaaaaaaat cgttgtaaaa acaacaaaaa ggaacttatg aaatcttacg atccgttact
    45601 gctgcaaatg ccgctctttt ataacttttc ccccaaagat ttggattcgt tacgaaactg
    45661 cttatcgttt cgggtccgga aatatctgaa aggggaagcg atttttttgg aaggtgatga
    45721 aatctcgcga ttgggaatta ttctgaacgg gagcgttatt attgtcaagg aggatatttc
    45781 gggaaatcgc aatattattt caaccttttc aaccggaaat ctgttcggag aagcttttgc
    45841 attttcaaaa caaaaacgga ttatggtttc tgttttttcc gaatcggact ccgaaattct
    45901 atttttggat gccgaacatt tactgacact ttgcaaagaa ctatgcccgt tccattctca
    45961 actgatccgt aatttgatga atgaattcgc ggaaaaaaca attctgttaa acgaaaaaat
    46021 cgatttatta tctcaaaaaa cgattcagga caaagtgttg gcgtttcttt tcagcgaagc
    46081 aaaacggcag ggcagccgtt ccgtgcaaat tccttttaac cgtcagcaac ttgccgatta
    46141 tctgtttgtt gatcgcagcg ctctttccgc cgaattaagt cgtatgcaaa aaaaaggaat
    46201 tttggaatat cataaaaata aattcgtcct aaaaacgata gctcatttgg atgacaatcc
    46261 ttctttcaat ttgtaaaaac aagattgatt ccttgacata tagaatattt gttctataat
    46321 cagaaatatg gaacaaaaac tttctttttg cacatctgaa attctgattc ggaatatgcg
    46381 ttccccgctt ccttatgggt tgacggttat tcaagggcct catgccgcca gcgggctttt
    46441 actgcagatt tgcgcaaagc tcgcattgag aggtcctgtg cggattttag actgcgggaa
    46501 tcgcagcgat atgtatcagg ttgccagaga attacgtccg ttgacacgtg atccggcaac
    46561 cgctatgcag cgaatccact tgtctcgagc ttttacctgc tatcaagcgg aagctctgct
    46621 gggtcagaac cgcggacaat ctcaaacgcc gattctgatc tttgatttat tggcaacata
    46681 cttagacgaa agcgtttcca aacaggaagc cgaacggtta tttgatgaca cgttggatca
    46741 tctgcaaaaa atgagccgaa aaaacccggt attggttggg attcgaccgt tgccggcgat
    46801 tgctcttgac cgcagcaatt taccgttgcg actgcaaaaa gccgcggatg aatcttttca
    46861 gcttatggag atatcacaga aagcggaaac aaatgatgat caactgtcgt tgttttgaaa
    46921 cagcggaagg agcgatttta tgggaaggac aattcgatct gctacgcaaa cttggataga
    46981 ggaagagaaa gcgttaggac gatttgcacg tgctttgcgg aaagaagatc aacgtgcaat
    47041 gatggagctc attaatcttt cgcggcttca tatcgcggaa tcttcctatg ccagcaacct
    47101 ctatccgatg gatgtttatt taatctccat gcttttggag atgtttaaaa gagtacagcg
    47161 tgatgaagca acgttggctg aggtctgtaa acatgccggc atcgaattgc cgtctgtttc
    47221 gactgaaatt cccgaattcc cggatttggt cgacctgatt gaatcgctgg atcaggctga
    47281 tgcgtaactg ttttgtctat gcaaatgcat gccaggggtt ggctgcttga cctttatgaa
    47341 gatgcgaacg acggcgttcg actttggttc attttggaaa atgatcaacg gatttgttta
    47401 aaacaacaat tgccgattac attttatatt tccggagatt caaaacgttt acatcagtgt
    47461 tgcctctttc tgaaacatct gccgggcatg attgcaatgc aacgtgtcag caaaaaagat
    47521 gtctttaaaa ccgagccggt atgtgcatta caagttgata tggccgaccc tgttcatcag
    47581 cgtcaagcat ttttaaagct taaaaaagat tttcccgatt tgacttacta taatgccgat
    47641 cttgccgtac atattcacca tgccgctctt taccaaactt tcccgatggc gttttgtgat
    47701 ttattatacg attctgaaag cggtttgatt caaatgttaa aagtgcttaa cagccgttgg
    47761 gatattacgc ctctgcaccc cgtgttacga atccttgaaa ttgcgcccga ctgtgatccg
    47821 atccgaaaga aaccggaaat gctaaaaata tgcgaccgcc gcggccataa acaaacaatt
    47881 tgtttacggg aaccgacact tcttttggag catctgaacg cattgatagc agcaaccgat
    47941 ccggatattg tggaaaccca atggggcgat gactggttgt tcccgttgtt attgcagtgg
    48001 gaacaggaaa cgggaattcc gctgaatttt aatcgagacc cggaacgaaa gcttcgttgg
    48061 aaaaaagaaa taacttattt ttcatacggc caaattgtat atcggggacg ggaagcgcat
    48121 ttattcggtc gctgccatat cgatgttcgc aatgcgatta tgtggaaaga ttacgggatg
    48181 gccgggacat tggaatcggc acgggtaacg acattgccga ttgagcaggc ttcacgtgta
    48241 tcgcccggat cgggaatttc cgccatgcaa atgatcacgg cactggaaga agacgtactg
    48301 gttcccgagc aaaagcagca ggtggaattc acaaaaacag gactggatct gatccgctcc
    48361 gaccggggtg gtctggttta tcaaccgcgc accggtttat attgcgacgt ggcacaaatt
    48421 gatttcacgt cgatgtatcc cgctgtgatt atcaacggca atatctctcc ggaagtgccg
    48481 cttcctcagg ggctgaaacc ttccagtccg aatctgggtg ttgttccgtt gactttaaaa
    48541 ccgctttatg aaaagcgggt aaaaataaaa caacgactgt tggaaatgcc ggataaggaa
    48601 ggaactgtcg caaaaagtta caccgccaga gcctctgctt tgaagtggct gctggtagtt
    48661 tgcttcggat ttttaggtta taagaatgcg cgattcggac gaatcgaagc acatgaggcg
    48721 gtcactcgcg gcggccgcga ggcgctgttg ttagcaaaag aggcttgcga agatttcggt
    48781 ttcgaagtgc tgcatttgtt tgtcgatgca ttatggattc gtcagccggg ttatcgggcg
    48841 gttgcggatt ttcagccggt tctggatgaa atttctcgca gaaccggtat gatcatatcg
    48901 ttggacggca tttttcgttg gatcgcattt ttaccgtccc gcacgaatga taatcaaccg
    48961 gtgccgaatc gctatttcgg tgtatttaaa gatggaagcc tgaaaattcg ggggattgaa
    49021 gcccggcggc gggatttacc gcgctgggta attgacaccc aaacggatat gttaaattta
    49081 ctggcaaaag cgactgatgc tcgccaactc ccggattatt tgccggacgc atttaaaatc
    49141 tttacccatg ctttggatga gctcaactcg gatcgggtac cgttggaatc attggtcctg
    49201 accaatcgta tcagccggga tttggaatta tataagtcac cgacggcagc ggtacgggct
    49261 gcgcttcaat tgttcgaaca gaccggaaag cgcacggatc cggggcaaaa gattcgttat
    49321 ctttatacaa aaaatgatgt ttatgcttgg gatcgccccg gaagtctttc cccggatcag
    49381 attgataaat caaaataccg agaaatgctg gcgcgggccg ccggtacggt tttttatccg
    49441 ttcgggattg attcgaagca actttcggca tatgcccata aatcgcttca gtttgaatta
    49501 agcttaatga aataaaaaag accaaacgtc tttttttgat aggagtttgg tctttctgaa
    49561 ccgaaaagga tcgagattaa cggaccgcct tcggattttc cttatcttcg gacgaatcaa
    49621 aaatatgaaa cgcttccata ttgaaaacca gtttgacttt gtcatgaatg gaatagtgac
    49681 tgcggggatc tacccgggca acaaactgtg tatcgcccgt catacaatac aagaaaattt
    49741 cattccccat taactcgatt acatcgacta ccgcttcaat ctcagcggtt ttgattccgg
    49801 gcggacaaaa ttcgggatta tggacatttt caggacgaat accgaagata atttcttttc
    49861 cgtcatatgg tgcataagta tcttttcgat catccggaat ttgaacagtg aacgaatccg
    49921 tcacaacgta tacattccct tcttcaactt tgatttttgc attgaaaaag ttcatggcgg
    49981 gagaaccgat aaacccggca acaaagagat tatccggtct gttgtacaaa ttctgcggtg
    50041 tatccaattg ctgaataatt cccttataca ttacggcaat tcgatccgcc attgtcatcg
    50101 cttccacttg atcatgggtt acgtaaatga aagtggtttg aagccgttgg tgtaatttgc
    50161 tgatttcaga acgagtctga actctcaatt ttgcatccaa gtttgacaac ggttcatcca
    50221 ggaggaatac ttgcgggtta cggacgatgg cacgtccaag cgcaactctt tggcgttgac
    50281 cgccggataa ttcacgcggt ttccgttgta aaagcctttc caagcccaaa gtcgccgcag
    50341 cttcattaac acgccggttt atttcttctt tcggtgtttt gcgaagtttt aaaccaaaag
    50401 ccatattatc aaaaaccgtc atgtggggat acaatgcgta ggattgaaat accattgcga
    50461 tatcccgatc tttcggagaa atgtcgttta ccacacgatc cccgattaat atttcgccgg
    50521 atgtaacttc ttcaagtccc gcgagacatc gtaatgcggt tgatttgccg caacccgacg
    50581 gacccaccaa aaccaaaaac tctttatcgt ttattgcgag ctgaatgtct ttaagtactt
    50641 caacatcgcc aaactttttc caaacatggt cataagttac gcttgccatt taataaattc
    50701 ctccattccg tatgggtata gtggctttat tatacgatga aacttattaa aattacagag
    50761 agaaattttc gacgatccgc gattatatat ctttcaacga aaaacgataa aaaagacccc
    50821 gccatgccgt gtgctgctga gttttgaacc tgctatcgca ggacggtttc ccgttttcca
    50881 aacgcagcct tggctttcgc ctatcgcatt gtcgggaata ccggaaaacc catttttact
    50941 cgtgttggcc caaaaccgtt tttacagcat cacctgcata gcagggtatg tctgttttat
    51001 accataaaaa cgaatcattg aacaggcaaa aagcccttat cttgaaaaag aaaccttctt
    51061 gattttgccg tcttttgaaa cggttacccg cgtaattaat cgatcggtca tgtttcccaa
    51121 tttggcatct ttggtaaaaa caacaacata gttgggactt tcaatatatc gctgtcgatc
    51181 gtcttgtgat tttgcactta ggatttgctt ttgaatggta aaggaagaaa attcatcatt
    51241 catcccttcc attttgtaca tttcctttac ttcttcatag atctttttag ccaagtcggt
    51301 gtcaatagcc actttactgt ccaatcgttc atggatcagc tcataacttc tcggagtttt
    51361 agctctttgc attctgtcgg ttatatcagc attatcatcc acagcatata acgggtcacc
    51421 caaaaaaata aaagaaatta atgttttctg aatttctccg tctaatgctc ccgagttgga
    51481 ttcggtttcg gtcgctaagt ttttcttggc acgcctgaat gcttctccgc atgaaatacc
    51541 tgtcataaga tgtttccaaa acagatgagc aagcaagtcc gcagcggtta aaggtaaact
    51601 catcgcaccg taggcaataa cagttgatcc gataaagaca cgcgttcctt ttccgatcat
    51661 atgtaaagag attgcatttg attcgtttct gtttaaaatt tccgcaccgt aacaattttc
    51721 ggcaaaaaca actttcggtg tcttagtaac aggttgaaga ttttgaattt gtaaagcgac
    51781 cggtatcatg ggaatggtgg aggtatcttt tgaatctttt tgtccgtacc aattcggttg
    51841 gccctttatc ccatgaagat taaaatatcc gaaatcgatc ccttccagtc gggaggaagg
    51901 aaaattggtt gccatggtcg gaggagaaac caacatctct gaactgtccg tcagattacg
    51961 gtagatcgca acggacggac gttgccaaac ggaacagcaa tagccgaagc ttttcacccc
    52021 cttcttgatt ttggcaccgc gaggagcttt tgcggatttt ctctttgatc gggattgttc
    52081 ctgttcatat tttgctatgt gatagttctg aattgaacga agttgactca acaataagcc
    52141 ggggtcatcg gaactgtcac ccggaattcg tcccacctgc cattgctgat catagtatcg
    52201 ggatgaatcc gggcttccgt atggagcatc tgaacaaatt gataaatcat catccattgc
    52261 cggatttgat aacttgaaaa acggaacgac atcatgtccg ccgacgatca ataacgctcc
    52321 gatcatcgaa cctttttgtg cgagtgattc gtctaattta ataatcgagt ttcggataga
    52381 ttgcggatcc aaaaccgttt tgctgtttgc ttggaattca tccggataaa ataccatcgc
    52441 attccagcca tttcgatttt caatcgcatc tgcggcggat cgcatttctt gatcgataaa
    52501 atctttattg tttttcccgt attttgccgt caatcccgca acggtcgata atattacata
    52561 aacgggaaag cgttcgtccg cgtcatcggg atctaatttg taaataaatt ccttaatcga
    52621 gtcgatggtt tcagtttgtt tttcccatct ccctgtaaat atttttttta aaaagccgat
    52681 taatgaagat aaattgccgt tagaagtaat aagtgtcttc atttcttttt gaggagagat
    52741 tttttctctg tccgaaagct cggactcctc ttgtttgatc attgattcat ccgcaattgc
    52801 aataggattc ggcaatgatt cgtcattctt ttccggcgct tccataaata catcttttgc
    52861 atcggcagcc gttatgaatt catccgaacg gttcgataat tttgaatccg aatcactcca
    52921 tgaaatatta atcgtatgat ccaatctcgc ttttttaccg actttcaacg ggatttgaat
    52981 ttttgaaaca tcaatcgaaa gagcagccga atcaatccaa agatccttat atacgttgtt
    53041 ttctttccaa gttcggttgg caacctggcc gagcagatca ttttccaagg tggaatgaat
    53101 aagtttggtt ccttcttcaa cgttacccat cttgttcatc acatcaccca gcaaaagttt
    53161 gaattgaatc gcgttcggcc aacggcgggt ataaattttc atcaaccgaa aatatgattc
    53221 gagatctttc tgttgatcac tgttcagaga aaaatgaatg gccgtaataa tgtcaaaatc
    53281 atcgacgtcg tcaatacatt ccaaattctt ttcggttaat cctctattaa aaaatccgaa
    53341 aatattttta ttacgagaac cttctttacc tttttgatag ctttttatta atttcatccg
    53401 gctgagagcg attttcttat cttcgctgtt ttccggatac aaaacagaca gcaatgccca
    53461 tacttcaaag aattccggat catatttcaa aataatttcg cttaaccgta tggcttcatt
    53521 tgttttcccg gatttataaa gaactattgc cagacggtat ctgatccata aatcatttgg
    53581 aaattccttt aaccaagcaa ttaacaattt tcttgcacaa tcaaaatttc caagttgaaa
    53641 agctgcattt gataaatcta ttaattgagt tcggttaatc ttcgcttttt ccattcaagg
    53701 gcctgtctta ttatgaagtt aaagtaaaaa gagataacac aaagctgcaa gaataaaacc
    53761 gatcagaacc aaagataatc ccaccccgcg ctttgtttcg atcagtgtgt ttaattctcg
    53821 gattaaagta ctggcattac cgagcgctcc gctcatttca tctgtaaaac cttttgaaga
    53881 caacttcgcg gcttgttttt gaatctcctg agcatttttt gaaaaaacgc tggaaatgat
    53941 tatgacaatt cccataatta tcgctataca actgacaatg aacagaacga ttccggctat
    54001 tgagaaggta atagaagtat taatcataat ttgacgaccc tccctttatt cgaattttgc
    54061 gatgtccgct ggaatatcgc ataaattgat cgtatctcct tcacaaaaaa tgcttgcacg
    54121 ttcaatagca ttttttagtt cgcgaatatt accgggccat gaataattct gaaatgctcg
    54181 gagaaccggt tcagatacct tggttatatt cactcccctt tctgcactga tagactttac
    54241 gaaaaacccg atcagctcag gaaggtcttc ttttctttca cgtaaaggcg gaatctcgat
    54301 atcgactatg cgcaatctgt aatataaatc ctcccgaaaa tcgccttttt ctatcatctc
    54361 gggaatattt cgattcgaag cggcaacaat ttgcacatca acgggtattt ctttggtgcc
    54421 tcccacatgc ctgactttct tttcttctat ggcgcgcaac aattttgact gcatatccat
    54481 gctcattgag gagatttcat ccaaaaaaag aatgccgcca tcagccactt caaataaacc
    54541 gggttttctc ttttcagcat ttgtaaaagc accggcttca tatccaaaca actcagattc
    54601 aagaattgtt ggttgaatcg cagcacaatt gatgggtacc agcagtttgt tcgctctcgg
    54661 gccgttctga tgaataaatt tagccagaac ctctttaccg gtacctgttt cgcctccgat
    54721 caaaatagaa acctgagctt tcgccgcaag ttgagcatct ttaaatatcc gtttcatttt
    54781 ttcattctgt ccgacgacga atgaaaaatc ttttttatag gcacccatta aactttcaat
    54841 ttctttttga agccgaataa catcctccgc gcgttttaaa gttgcctcca acagcggaaa
    54901 gcttaccggc ttcgaaagaa aatcaaaagc accgtttttc attgcgtcaa cagccatttc
    54961 aacttcaccg tatgcggtta ttaaaatagt tttcggtgtc ggacgaattc gattaatgtc
    55021 ataaagaaga tcaggcccgt attccttgcc gacctgaaca tccaacatga tgatatcgac
    55081 cggttgacgg ttgagaattt cgcgagcttc gcttaaagat gcggcatcca gtatttcata
    55141 gttaacatct ctcgaaagat attgtttaat cccttctcga aaattttctt catcgtctac
    55201 cactaaaaca gtaaaatcca tcctcttata ctcccgtact gatcggtaag gtaatttcaa
    55261 aaacggtacc gccggggtat gattttactt gtattgtccc cttatgtgag gtgataatac
    55321 gtttgctgac tgcaagtccc caaccgtttc cttttttctt tgaagtaaca aaaggttcaa
    55381 aaatatgatc aattatttca tctggaattc ccggaccgtt atcggaaact gatatttgaa
    55441 cctgtttcct ccctgtcttt gaatcatgca aactgatttt gaaagaaata accccaccct
    55501 tattatcgaa tgcatcgatc gcatttccga tcaggttgtt gaatacttgt tccatagaac
    55561 gcgggtcgat caatactttc ggtaaatttt cgggtgcgct gaaaaaagtt ttgatattcg
    55621 ctcgcgcaaa atttaatttc cagcgttcga taatttcatt gatatactgc tccaaatcta
    55681 tttcttccaa attcagcttg agtggtttgg aaaaagccag aatattatta accagtacgg
    55741 agactcttga acaatcttcc tcaattcgat ttactgcttg ataaatatca ctttgttcat
    55801 tcgttaaact ctttatattt tgaatccaga tattcatatt gttgatcgga tttcgaattt
    55861 catgggctaa taaagatgca aaatcaccta aaaatgctcg ttgggtaagt tgttctgttt
    55921 ttagacggct ctcctcggat tctgatacat cggttaagac aaaaattgta tagctgtttt
    55981 tttcatcaaa atcaagcgtt gaaatccgga gatgacaagg gaagctttct ccacttcgac
    56041 gatgtaaata ttgtatgaaa ggtgtttcat ccgagttatt ttccgatgaa gataatgttt
    56101 ggacgatctt tctgacccct tcatttgtta ccagaagatc atctaaaggg tgtccgacaa
    56161 catcggaagg agcaaaaccg aacatgatcg atgatatttg attgatatat acgatttgat
    56221 attcattatt ggtaataatg atgccttctg aaatattatc gataatctga tgatttaatg
    56281 aattttggaa ttcgcattga tacaacgact tagtcatctc catattttcg aataaaccct
    56341 gaaaatatat actcaggatt tcaagaaccg tctgataaaa atccagacga tataaagcgc
    56401 tgtcctgaaa tcggagaatg aaatagccga tcctttgttc ggaatttgac acgggaaata
    56461 ttttaaaatg aatgggactc cccgcaaatt catcggaaac atcacttaac ctgtctccct
    56521 caaaagaaga aaaatgactt agattttctt caatataggg atagattgat tttgacatga
    56581 tattcttctg caaccggtca taaaggtcat attgctcgaa aatgggttcg ggaataaatc
    56641 cgaattcgga aaggcctaat tctaccgaag tcatgtccat gaatgattca aaaccctcca
    56701 aaaaatcagt ccttgaacag aaggtgaccg aagcatttcc gataatatgc aataatgcat
    56761 ccatggattg acttccggtt gtttcgatgt tccagcgcgg attaatgaca accgacgcca
    56821 atatcgtttt ttccggtgtt aaataggaat tcctgacctg aacaggcttt attcggccgt
    56881 cttgtgatcg aaattcaaac cacatggtat gatggtcttc atcgggtatc tctttccagt
    56941 tgcgaatcag atcagaaagt tccgcgttat caaccgaaga acggaatgaa ttgagaaatt
    57001 caactcccat gggatttgcc gaaatcaacc gtccttcttg aacagcccaa ataaaagcgg
    57061 gagtgataca agaatataaa aatgagtcgc aaatggtctt gacttcaaaa gagttttcaa
    57121 tcgagatatt ttccattcgt cttatctcaa attagcgctg tttctcatat gagccggtaa
    57181 aatgccttcc atggcacgat atttggcaac cgttctgcgg gcgatatcga ttcccatccc
    57241 ggttaatact tcgacaattt ttgcgtcagt cagtggtttc ttttcttctt tgaccaaatc
    57301 tttgataatt gttcggacat tcagacttcg atcaaaaaat gctgacaagg ggacaacttt
    57361 tttattggga agcataactg ttttattcgc aaccgctcga ctgattgtag attcatgaac
    57421 atctaacaat ttcgatatct gtgcgcgggt aatcggaaca atattgctgt ctccgtatcg
    57481 aatgaaattc ccttggagat cgaccaaaac ttccatcaat cgcaccattg tattatttct
    57541 ttggtggagg catttaataa gcagcgaagc acggtcaata tcatttttcc actcctccac
    57601 cttatcttcg ggcgcatttt tcgattctct tttgaaaaat tcattcactt tcagattccc
    57661 gctgattggc ataattatct ctaccacaag cggattcttt ggattatcgt tcaagtaata
    57721 aattaaaata tccggaaagt gataggtatt attgtctaca ttattatcga ttcctttttc
    57781 cccccaataa gcacgcccgg gataaggatt cagattttca ccgataaatt tttcaatcgt
    57841 attaatttgt ttggttgtga gatcatggat ttttgaaatc tcccggtatt tcccgtggat
    57901 taataaatca taagaatcac gaataacatc caaagtctta tcggggatat cgattaatcc
    57961 ggataaatgc tggatttgaa ccattaaagc ttcttgagga ttgcgcgaac cgacaccaag
    58021 gggatccgca tgctggatta attgaataac ctcctcgatt tttgaaggaa gtacatggta
    58081 atactgagca atctccaata cagaggaagt tataaatccg tcttcatcaa gacttgtcag
    58141 caaataagcc gcgatcattt tttcatcatc ctcgagatcg ggacctattt gtcgtaaaac
    58201 ataagtcggc aaatcttcat gctgcgcgga atccatatcc ggttcatgag ataattccga
    58261 atggaccgca taattttcaa ccgcaaaatc atcacgcggg ctgacgaaaa caatgctttc
    58321 ttcatcttcg gattttaagg tgcaacagtt agggcaaata ccattgtcgg gaagcaaacg
    58381 atgacaggtc ggacaaattc tttcatcagt gagttccaaa gccggattta cagataattc
    58441 actggctatt ttttcgcgaa tttcatcggc agacatactg agtattgtca tggtttgtgc
    58501 taaatgtgct gaagtttgct gacgtaattc ttgcttttga cgttgataca taatttattc
    58561 ctcattattc cggcaagttt cagaaatccg tattcgttat tattcagaaa ccattcgttt
    58621 aaactatagg tgcaagtttt ataccacagc acaagaattg taccagactc aagtattctt
    58681 caaaagagtt taaaaggagt cgctatgatt tttaattctg aatattccgt ttattgaata
    58741 ttcttattac aacgcgatca aggagaaaat ccatggtaaa aatgttcgat atgggaagca
    58801 ataatcaatc cgatttatgt ataatcggcg ggggcttagc cggttgtgaa gctgcatgga
    58861 ctgctgcaca aagaggagtt catgtcattc tttacgaaat gagaccacaa aaaatgaccg
    58921 aagcccatgt aaccggaaat ctggcggaat tggtttgttc caattctttg ggatcaaacc
    58981 tgatcgatcg cgcttccggc ttattgaaag aagaacttcg ttttttgggc tcacttctga
    59041 tcgatatagc ggaaaaaaca aaattgcccg cagggaatgc attagctgtg gatcgtgaag
    59101 cattttcgat cgcgattgaa aatgctgttt tgagtcaccc gaatatacag gtaatccgtg
    59161 atgaagtgaa gtatattccc gacgatttac cggtaatcat cgcaagcgga ccgttgactt
    59221 ccgaatcgtt atgtaaagat attgtccgct tgaccggaga agagaatctc tatttttatg
    59281 atgcaatcgc cccggttgtt tatgccgatt cgatcgattt taagattgct tttcgagctt
    59341 ctcgttatga ttttgacaac tccgaagaag gagattatat aaactgtcca ttctcaaaag
    59401 aagaatatga tcggtttgtc gatttgctca ctcatgccga acggattgag ttaaaagatt
    59461 ttgagaaaga tatcctttcc ggtcagcaga aatttttcga aggctgttta ccgattgaaa
    59521 tattggcagc cagagggaaa gatgcgctgg catttggtcc tcttcgtccg accggattga
    59581 aagatccaca taccgggcat cgtccacggg cggttttgca gttacgacaa gataatctcg
    59641 cgggagattt atataacctg gtaggttttc aaacaaatct tcgctactcc gaacaagaaa
    59701 gagtatttcg aaatattccc gggctggaaa atgccgtttt tgcccgcttt ggtcaaatgc
    59761 atcggaatac attcctttct tcccccggct tgatcaatga acatctgcaa ttaatttccg
    59821 atccgcgaat attctttgcc ggacaactgg ccggcgttga aggatatatg ggaaatattg
    59881 gaaccggttt ggttgcggga ttaaatgcag ccgcattcat ccacggagat ccgttacccc
    59941 ttttccctat agagacaatg ctcggctcga tgattcatta cttggcaaat tcagagaaaa
    60001 agaattttca gccgattaag gcgaatttcg gcatattacc tcccttagag aaccctcccc
    60061 gatcaaagca ggaacgggga aggcaatatg caattcgctc tttagataca ttaaaaaatc
    60121 ttcgatcgta ggccggttga attattcttt tttctcttta tcgataacat gaacttcagt
    60181 tgcccgtagt ccttttgaac tttctaccgg aataaattca acctgttgac cttccgacag
    60241 atttttaaat ccgtccgacc ggatatccaa ataatgaaca aaaatatctt tttgacccgg
    60301 aaccgctata aatccgtatc ccttactcgt attaaaccat ttgactgtgc cggttaattt
    60361 ttcactcata atatgatctc ctgaaattct tgcttgataa aaagttcgac ggatatcgca
    60421 tttactcaat cggcctgctg atccatccgc caattaataa caagccgata ctgacaattg
    60481 ttcccactcc gaaattaatc gttccgagtg ttaaaaattt tattcccatc aactcggcaa
    60541 tatattgacc gccaaaaaag ccgatgttag agacaacaat gtaaataaaa agggtaaaaa
    60601 gattcccgcc tctgattaaa tggaagatac acgcatataa agtagccatt atgaaactga
    60661 aaataattgc gggtagtatc attattttct ttccgttttc aaaattctac cactgcctat
    60721 gacttcgcct tccgtatcat atagaaccgc aatttgtccg caagccactc cgtcaaagaa
    60781 gtcatctttt aaagaaatga tcattttctt ttcctcggtt attcgaacag aaacgggcac
    60841 cgcctttgac ttatatcgga actgggcaaa acaatcgaag gaatcaaccg tttgttcgtt
    60901 gcagatccaa ttaatatcat cggcaaccac cttttttact ctcaaatctt ctttttgacc
    60961 caaaacaatt tcattacgaa ttacatcttt tgcgatgaca tatagcggat tctttgcgga
    61021 tatttttaat ccttttcgtt gtccgattgt ataaaaagga atcccgtcat gctgcccaat
    61081 ttcttttcct tccgaatcga taaattttcc cggcgaaatc cgttgcggct gcatatgttt
    61141 tagaaatctg cgataatctc cgtcaccgat aaagcagata tcttggcttt ccggtttttc
    61201 gctataagga agatgatatt cagccgaaat ttttcgaact tgggacttga aataatcgcc
    61261 taccggaaaa acagaacgtt ggagttgcct ttgagataag cgactgatta cataagactg
    61321 atctttattg ggatccgttc cgcgaaataa gtgggttatt cctctctcat ctttaatggt
    61381 gcgcacataa tgaccggtag cgacatactc cgcatccatt gcatcggcat attgcaataa
    61441 ttgtctccaa cgtaccaaag ggttacacca tgcgcaggga ttgggagtct ctcccaaaaa
    61501 ataagaagaa ataaacggtt gcaccacaga ttcatagaat ggttctaccg catccaaaag
    61561 gtagaaagga atatcaattt tggttgcaat ccgatgtgct tgattcgcag cttcaatcga
    61621 acaacattgg ttttcacgat caaagccctc ttcagcccat aatttaagca ttacaccgat
    61681 tacatcaaat ccttgctgtt ttaataacaa tgcggccaca gaactgtcca ctccgccgct
    61741 cattccgacg attactcttt gttttttctt gatcattatt aagaacgccg taaaattctg
    61801 atcgatcttt ttagagcatt taccaatgaa taaattgcat catcagtagt atagataccg
    61861 actgtaattc ttaatccgcc gtttgcccac tcgctgctca agccaatgtc gatcaattgt
    61921 tgttgaccat gaatttttga ggaacgacat gccgaaccga tggaaacagc aaatcctttt
    61981 tgatccagca aggattgtaa aacgagacta tcaatacctt tgaaagcaaa actggcatga
    62041 ttcgtcaatc tttcagtcgg atgaccggtt aataggcagt cgggaatgtt ttctgagatt
    62101 aattgaatca gcaaatccct tttctgtcgc tcaaatgcga ttcgcttgtc aaattcatta
    62161 tcaagaattt gatatgccgc agccattccg actatataag gcacattatg ggtccctgct
    62221 cgtttgccgt tttcttgttt tccgccatgt atgaaaggta aaaaattttc cacaccatta
    62281 ccgagcaaaa ttgcgccggt gccttttggc ccatagcatt tatgagccgc aatattcatc
    62341 attgaaattt cggaatttcc aaaatcgatt tgattatgag caacatattg tgtggcatct
    62401 gtatgaaaag gaattccttt ttccttacag actcgagcaa tttcaggaat cggattgatt
    62461 gttccgattt cattgtttcc gtaaattacc gaaaccaata ccgtatcaga gcgaatcatt
    62521 ttccttacat cgtccggatc gacttgccca tattgattaa ctggtaattg atcgataata
    62581 caaaaaccgg catcttgtaa aaatcttgca gtttcattaa cggcgctatg ctccaatgga
    62641 gaaatcagaa gacgaccggg tttgccgtaa attttccaat attgaaagac tgttgcccac
    62701 agaattgtat tgcaggcttc cgttgacgaa ccggtaaaaa tcaaattatc cggattcgta
    62761 atatgaaagt gatcgagtag aatattacga gcatcttcga ccgctccata agcttgttga
    62821 ccgaatcggt gggctgacga gggattccca aacagatttg taaaataggg ttgcatgcgt
    62881 gtaaaaactg tcggaaggag cggagtagtt gctgcgtaat cgaaatagtt ttcaatattt
    62941 aaccttttca taactcgatt atattcagtt taaaagattg cggaaataat ttacaattaa
    63001 tagagtatag aagaaagaaa gctgtttaat gagacttcaa agaggataaa tggaccagat
    63061 ttttcaattc tttaatcaaa ttatttcttg gttttccgga ttatcgttgg cagaaaagtt
    63121 gggagtaatt ggttttatct tatcctccct gatctcaatt atttatataa ttaaatggat
    63181 cgctcatctt ttccgtcgag aaaaaaggaa agtatcgttg agttttaaac ttgaagacgt
    63241 tacacatgaa atgtttaaaa agaaaggttt tgtcactttt gatctttatg ttcgaaacga
    63301 cggagattca cccatcgaaa tccttcaatc cggattcatt ttagccgatg gaacagaaat
    63361 accaatttat gaggaacccc ataaaattaa ggctcccgat aatgttatcg ttcaaccagg
    63421 acatgaagct ttttatgata attgcgattt gtttcaaatt ttgaatgtta acggcgtcaa
    63481 ttataaaaaa attgtcggtt tttatgcgga tgtaaaggga gcgcctcgct ttcgaacaga
    63541 aggtgatatc cacccattaa tgaatgatca gatagttcgc ggaattttga cggataaaaa
    63601 aggattaaat ggattctgat caattttttc cggaaaaagt ttgtggtaaa atttctctgc
    63661 aatccgttca agcgggagta gccaagtggt aaggcattag cttcccaagc taacattcgc
    63721 gagttcgaat ctcgtctccc gctcttcttt cttataaaat gaaacagtaa caaatgattc
    63781 tgtttcattt tttatatcga ttgattccaa acatcagaac cgaaataata cttaggatag
    63841 aaattactaa accgattcga aatcctggcg ggataaattt taattgaatc tcatgtgcgg
    63901 attcgggaat attcttaata cagagtaaac cggaaagtat atcgtcatat cttattcttt
    63961 ttccgtcgag aaaaacttgc catcccgatt cgaagggaat cgaggaaacc agaataaggt
    64021 tgtttgcttc ttgattcaga agaattttaa atgaattaaa tccggtttgt ataacctgtg
    64081 gagcatcttc ttgaatcttt tttgtaagaa tcgaaaaacg atcgatatca aattcataga
    64141 cagatggaag ttgaaattct tgccaatctt gccgatgaaa attaatctgg ataacggagg
    64201 attctctttc ttttaaataa ggataaagat tgaatatata ttggattccg ttactcatat
    64261 agggatctat atcaaccgat gttccgttga ttaataattc gtatccgcca atcacctgcg
    64321 gaataaacaa ataatatgga gaaatccttg aagttttaaa tcgaatggtg atcgaagaag
    64381 gttgagcggg atttaaaact cgtacctgat ctttttccgt atctgaatat accaagttat
    64441 cgagcgatat atcttgtatc ggcacacttt cgtaaaatcg gccggtttct cctgctattc
    64501 cttctaatat tgcttcttga aataaaacgg ggttttcata agcggaagat ccgactgaat
    64561 ttaatgcgga atctgaataa aagaacatcg gaaatggata cggattcgac caaacaaaac
    64621 tttcgatttc atcataatca tggttcaaag cataatcgga aaagagatac ttaataccaa
    64681 acagcgaatc tgtgaatata gtggatccct catatcgaat ccaataatta atcgaagtaa
    64741 tgcccagaga ttgagtcaac gcttgaaatt ttttgtttaa tatcgaggta tagagcgaaa
    64801 ggccgttata tcccaattcg attgaatcat ttgacataga acgtcgatct gttttttcaa
    64861 ttcgataaaa atctccagga atttttgacg aataacgatc gatcaaatcg gcatatttgc
    64921 gatgataatc caaataagaa ttacgatctg tcagaaccac cgaattttcc ttataaagga
    64981 gaatgccgtt caaaaagcat tcaatcgaac agaatatcca taagaaataa ggaatatgcc
    65041 tatttcgttt tccgcggttc aagaccacta aaaataatga ataaacaact atgaaaaaca
    65101 ggttgatgaa aatttgataa gactttattc tgtcaggata aatttgatga aaaataagca
    65161 agcctaaaat taaaaagaca ccacataaga taatatttaa attgtttata tgcagtttaa
    65221 tcaaaaattc aaaagaacaa gtcaacaata agaaagaaaa gacaaaagaa aaacgataat
    65281 gaaaccaatt gggataatca aatccatgcc agataaaatt gagaatttga tccgtaaatg
    65341 aaaggataag aatgatataa agtgcacccg aaaaaatttt tgtcttcttt ggaatctgtt
    65401 tgttaaggaa gaagaggcaa cctgatatca atggcaaaat tccacaataa atgtagggca
    65461 agttcgcttt gactccgtcg ccgattccga taaaaagctt gggaaataac tcaaaagccg
    65521 aaaaattaaa gtggtgaatt ggaaacggtt ggccgattaa atcagcattt tcttttaatg
    65581 ctctgaataa gggcagccat aaaaaagcgc tcaaaccggt tgccagaata atggaaatga
    65641 gaatgagtcc ggttttccga atcttttctt tccgagaaat cgacagatcg gtactgatga
    65701 tccttatgaa aaaataaaaa caagaaaaaa gacttgccat atacccgcaa taataattag
    65761 acacaattaa aagaaagtat aggatcacgc atccgcccca ttgatccctc gaaatgattt
    65821 tttcgatttg ccagcataca agcggcaata aaatgattgc attttgaaat gagaaaagaa
    65881 aataaaaagc aatcgaatac ccagacaaag catagcaaac agcaaaagga acaattatcg
    65941 gtttatttaa acagttgaag cgttttccga ggtagtatcc aaaggttaat ccgcttaatg
    66001 aaatttccaa taaaataatc actgtgaccg catcgatcat tcgctcttcc ggaaagaaaa
    66061 tcagaaggag ataaagcgga cttgagcaat aatgaacaaa tgtcgggaag aagtttatcc
    66121 ctccggcggc gtggaaagtg taaaaaagat ttccttctcc tttataaatt ctggctaatt
    66181 cggataaaaa cccgataaat tggtctgata aatcactata gagaagagaa ttgcttccga
    66241 aaggataaac tttttcaaaa gcgaggatgg caataaaaat cagaaagggc aaaagaaaac
    66301 aacaagctaa aatcttattg aaatgatttt ttatcggcag atttgagacc atttctttat
    66361 tcttttcata tggcgcaaac tattaaaatg cggctgatag catcactaaa ttaataatat
    66421 ccgagaaatc cattttacag agcaattcgc aaatcgtaaa aagaccgcaa ttcgtgttaa
    66481 aattctccca cgaaatggct tatcttaaga taatatcttc cactttttca ctttttgtcg
    66541 ccatcgcagt cggttttttc gccggaaaga aagattttct aaccgaatcc tccacaaccg
    66601 aactttcacg atttgtgatt cgaatcttat tcccgattta tttgtttcga tccgctgtga
    66661 cgtatatcaa taaggaaaat ttcctttcag ctccgcttta tacattctta aatgtgattg
    66721 tgatgatagc aatgtggttt atttccagac agatcgtgaa aaaagcaaat atttcgccgg
    66781 atagaatccc gatgtttgaa ttcacatccc ttgctgggaa taccggcttt ttcgggttga
    66841 cttttgccac acttctcttt ggttccgagg aaataatggc tgcgattctt tacgatttcg
    66901 gtggatcgtt agtaattttt atgattctcg tcccatttct tttagataac aatcatatac
    66961 atcatcctat aaattttctg aaagagccga acgttattgc cattttaagt ggtttgatat
    67021 tgggaatact tggggtgaaa attcctgaat tcttagcgcc ttcctttaat ttgctgagcc
    67081 aaatcacttt acctttggct ttgattttat gcggagcaca actggcatcc attaaaatat
    67141 caaaagaatt ccatttttct caagtactgt atgtcatatt aatcaaaatg attctgatgc
    67201 cattactcct gtttgtcatt gttttatgga ttcctttgga tgggcaaatg aagcagtttc
    67261 ttgtagttga tgctgcttta ccatcgggaa tttcaatggt aaatttcgca aaaacatatc
    67321 accaggattc tgatttcgca gcggtagctg tttttgcgac taccgctttt tcggttatat
    67381 ggcttccgtt tctactgatc gttttgtctt caatatttta ataaatcaat taattatttc
    67441 agaaattatt tcacccttat cgtaaatatt tcataaaaaa gcttaaaagt cccaacctgt
    67501 cataattcat tcatgacttt gttcagattg gttttttctt caaaaccctt ataatattta
    67561 taattggtga catttgaaat ttctgtaatt atttcaaagg aaaaatgaag ataacgctta
    67621 aacaaatcgc acgagaagcg ggagtttctc aggcaacggt gtcacgttta gttaatcaaa
    67681 acaccggtgt tagttcggaa ttggaagaaa aggtcacgaa cgcggcaaaa aaacttggcg
    67741 tggaattgcc gagaaaaagc tcggcagtga aatccgatca aaccaagaat aaagttatcg
    67801 gattgttggt caccgatctt tccaatccct attttcaagt aattatgagg ggcgttatcg
    67861 atgaagccag aattttaaat tacggagttc aaatattcga aacttatgaa gatataagat
    67921 ccgaagaaga agcgattaaa acaatcgagc gttttaaatt agacggtgcg attttatccg
    67981 cttcgcgtat ttcagaaaat aagctgatta atttccataa gaatacaaaa attccaatcg
    68041 ctttgatgaa tcgctatgtg aaagaaccgg aaatctgctg tattatcgtt gatttccaac
    68101 gatctttatt tcaagcaacc acccatttaa ttgatttggg acacaaaaaa atcgcctatc
    68161 tgtccggacc gtcaaacagt gatccttcga aaacgcgacg ggcagggatt gtaaaagctt
    68221 tggaacaggc aaatttaaaa ctcgaagcgg atttatgtgt cggaagtttc cccaatgtgg
    68281 aaggaggatt tcaggcaatg atctcgttgc tttctctctc tccagataaa cgtccgacag
    68341 ctgtacttgc ttataatgac ttgatagcgg tcggtgcatt gagagcgatc cggatgaaag
    68401 gattacaggt gccgaacgat atttcaatca tcggagtaga taatattgac atgtccgagc
    68461 acacctatcc gcctttaacg acgatttctc cgcctaaagt aaaaatggga tcgattgcca
    68521 tgagagcgat atatcgaatg aatatgggcg aatattatcc gaccgacgga tatatcgagg
    68581 tggacgcccc attaacttta cggtattcaa ccggaccggt aaaacataac ccgaacgaat
    68641 ctatttgatc acaaattcaa caaaggatgt gaaaaatggc aaaggaacaa tatgtttttg
    68701 aatcagaagg aaaaattatt cgttatcgat ttccgactca tatcaatgat ttgattatcc
    68761 ccagagagga gtcaacctgc gccgaagtat ttatggttgt cctggatgaa aatgaagctc
    68821 cccctctcca taaacatgat gatgccgaac agatttttta tatgattcaa gggaaaggca
    68881 aacttgaaat aagaaagcaa cagaatgacg aaccggaata tttcgaaatc aatgtcggac
    68941 aggttattaa aatcccgcct catatatggc atcgggtttt tgctttaagc aaagaaggcg
    69001 ttcgctacct gagcattgat tgtttcccaa atgggtttga tccgtccgaa ccgacctggg
    69061 atgatcatgt taaaaacatg gcaactgcca acggatggga cttttccaaa attcgtgaag
    69121 acagacgctg agaaaaattc gggcaggagt ttgatcagat gtctataaaa atccggaatc
    69181 gtattccgga atgaataaac caaataggag gaaaaatgtc aagaagaata tcaatattag
    69241 ggatcatctt agtttttgca acgatgttcc taatcgccgg tgctgtttcg gctgccgatt
    69301 atcatgaagc gccgatgctg gctgataaag tcgcagccgg cgaattgccc ccggttgcag
    69361 aacggctccc ggaagagccg cttgtcgaac aaccattgga atcgatcggt gtttacggtg
    69421 gtacgcttcg acgcgggatc accggaccaa atgaccacaa cagttatgtt cgttgggtct
    69481 atgactcact ggttcggttc agtcaggacg gaaactcaac ttatccgcat ctgatcaaat
    69541 cattggaacc caatgaagac tacacagtct ggacatttaa tatgttgaag ggcgcaaaat
    69601 ggtctgatgg gtcaccgttt acttctgccg acatcatgtt ctggtttcat gattattata
    69661 cgaatccgga tctcgcacct gccaaactgc cctggctgta caatgatgac ggctcgttgg
    69721 ttgaagtaac cgcaccggat gattatacgg ttgtgtttac gtttaaggat cccatcacaa
    69781 ctgtcccgat ggaagtggct gcaatggata accgtgataa tttggttccc atgttcctgc
    69841 cgtcagctta cttaaaacaa ttccatgcaa aatataccga tgtcgataaa ctcaaccaga
    69901 tggcgaaaga acaatctttt gaaaactgga aacagctttt tgagaataaa caagcgcctt
    69961 tccgcaatcc ggaaagacct gtgatggcag catggatgtc ggttaccacg attaatgatc
    70021 cgatttatac attagttcgc aacccgtatt atccgatcgt tgacacggag ggaaatcaac
    70081 tcccttatat cgaccaaatc aattttgtga cttatcaaag tacagatgct ctaaacatgg
    70141 ctgtcatcca aggtgaattg gatatgcaag accgccatgt tgtgatgcag aattaccctg
    70201 ccttggtcga acaagctgaa aaaaccggaa aatatgaagt tcgcttatgg ccgactttcg
    70261 gtggcgtcga tgctgcttta tcggtcaatt attattatga caaagatccg gattatatga
    70321 agttattcca aaatcatgat ttccgcgttg cattgtcact cggtatcgat cgggaagaaa
    70381 tcattcaaag tgcatttttg ggagtcgggg aagcacgaca accggtccca aggaacacct
    70441 caccgtatta tccgggagat gaatatgcct ataagtacac cgaatttgat caggcaaaag
    70501 ccaatgaaat tctggatggt ttgggaatga caaaacggga ttcccaagga ttcagattaa
    70561 tgcctgatgg aagccgctgt atgatcgaga ttgcatatgt accgcaattc gcaaactggg
    70621 gagatattgc acaaatgatc tctgaagatt ggcgcggaat cggaatcgac ggtgttgtgg
    70681 ttgaaaatga acgaaccaac catttcgcaa aacgcgattc caacgaactc atgaccgaaa
    70741 tctggaatga agacactacc ggattcccct tcaccggtgc gccaaaattc gatcctcgga
    70801 ccagcccggg tatttgtgtc gcaacgggtg ctcgtacgtg gcttaattca ggcggaaaag
    70861 aaggttatga acccaatgcc gatttcaaac ggattatgga aattatcgat tctgccaaga
    70921 gagtaagccc cgataaacag gctgaactcg gtaaggaact gttccgaatt tgggcggaag
    70981 gctgctatga gatcggaatc gcaggcttaa cgcctcttgt tcaaggtgtg gcggttatta
    71041 acgtcaacct gaagaacgtt cctgcttcca acgaactcgg taacgactgg ccgctccgaa
    71101 cccccggaaa cgcacgaacc gaacaattct attttgcaaa gtaatacaaa taattatggt
    71161 cgggcgattt tgcaaaatcg cccgaccggc aattttgaac tttattattc tttttattgg
    71221 aacgtacgag taagtgattt ccataaagaa aatcataaga ctcaaaattt ttatgtgatt
    71281 tttgaacgga gtaaacgatg ggaaaatttt tagcgaagag actccttttg ataccatttt
    71341 tattgctcat attttcgatc ataatttttg ccttgattca ggcacctccc ggcgatttta
    71401 tgaccactta ttccgcaatc ttggccggat ccggctcatc gttggatgcc gagcagttaa
    71461 atgcgatgcg ggtatcttat ggtttggatc aaccaatgca tatacagtac atccgatggc
    71521 ttggaaaact tcttcacgga gatatgggtc tgtcgttgtt atacatgctt cccaataagc
    71581 aattaatcgg tgaaagatta gggccgacta tccttttagc gctattttca ttcattcttt
    71641 cctggttgat agcgattccg tccggaattt attctgcgac gcataagaat tcgtttggtg
    71701 attacttctt tatggtaatc aactatatcg gagtcgccat tcccaacttc tttctggcac
    71761 ttttattaat gtggtttttc tattctcatt acggtttgaa tctcgtcggc ttgttttctc
    71821 cggagtttga gatggcgcca tggagttatg cgaagtttct ggatatgttg aaacatatat
    71881 ggttgcccgc tgttatcttg gcattaggag gctcagccag attaactcgg gtaatgcgtg
    71941 cgaatctgtt ggatgaatta gggaaacctt atatgattac ggggcgtgcc aaaggaatgt
    72001 cagaatgggc attaattatg aagtatccgg ttcgcttggc aattaatccg ttagttagca
    72061 caatcgggaa ttatattccc cagttgtttt ccggttctct gattgtggca acagtaatga
    72121 atcttcagaa tatcgggccg ctccttttgc aggcgctatc gatgcaagac atgtatttat
    72181 cagggtcaat tctgatcata tattgtctgc ttggtatttt aggaacaatt ctgtccgatt
    72241 tgttgttagc ttgggttgat cctcggatca gattggaata gtgaagatga cagaaaatta
    72301 ctctcccaaa aataattcaa atcccacaaa aaacaatccg tctgaatcct ctgttgagaa
    72361 ccaagcgaat attgatttgg atgctttggc cagcacaaaa agtgaagaaa aagtctccat
    72421 cgcttcgaac ggacaactga tttggtggcg ttttcgaaaa aataaaatgg cggtaatcag
    72481 cctttttatt ttagtaatcg tttttgctat cattttattc cctgattttt tcgcaaatca
    72541 aaattctgaa aagaccaatg cgcgtgaggc ttacattccc gtccaaagtt tacacttttt
    72601 caaagatgga aaacccgatc tttgggtcga taaagttgaa ggacaaagaa acccgaaaac
    72661 tttgggaatg gaatgggttg tcaaggaagg tgaatcggtc gatgtcaagt ttttcggaaa
    72721 aggaatgaaa tggaaattcc ttttttggga aaccgaacgt catttaatta caccatcgga
    72781 tcctaatact cgtgtgtttc ttttaggatc ggaccgtatg gggcgggatc agttttcgag
    72841 attgatgtat gcgacccaaa catctcttac agtcgggctt gtatccgttc ttctcagtgt
    72901 atttctgggt gtcctgttgg gtggaatcag cggatatttc gggggaatga ccgatatggt
    72961 cattcaaaga ttgatcgaaa tattaaactc tattccatat gtacccattt ggatggcaat
    73021 gactgcggcg ttgcctcgtc aatggtctgc cgaacagcgt ttctttgcta ttaccgtaat
    73081 tctttctctg ttgggttgga caacgctcgg aagagaagtt cggggaaagt ttatgtctat
    73141 tcgtgaggaa gattttatcc tttctgctga gttgatcgga tgcagtaaat ggcgaatcat
    73201 tatgaaacat atggttccca cttttctcag ccatattatt gcaaccggaa ccttatcgat
    73261 tccggcaatg atcgtaaatg aaacctcatt aagcttttta ggattaggcc ttcgtccgcc
    73321 ggcgatcagt tatggtgtca tgttacagga agcacaaaat gttcagacga tggctaaagc
    73381 tccatggctg atgctccccg gcttggtact ggtaatcaat gttttaatgt tcaatttgat
    73441 tggcgacgga ctacgtgatg ctgccgatcc gtacagtaaa taggagaaaa aatggaaaat
    73501 acaaaaccat tattatctgt acgagatttg aaagtttatt ttgatttaga cgaagggaca
    73561 gtcaaatccg tggatggcat ctcgtttgat gcttacgaag ggaaagtggt cggcattgtc
    73621 ggagaatccg ggtgcggaaa atccgttacc atgaaatcga tcctgcgaat tgtcgaaaaa
    73681 cccggaagaa tcgttgaagg cgacattttg ctccaaaaaa gagataatcc ggatgaatac
    73741 cttaacttgg tcaaaatgaa agaaaacggc aaagagattc gtgcggtccg aggaggagaa
    73801 attgctttga ttcctcagga accgatgtcg gcattcagcc cgattcatac catcggcgat
    73861 caaattaccg aagcgatcat gctgcataat ccggttgata aagaggaagc gcggagaata
    73921 tgtattgaat ccttaaaatc cgtcggtatg tctatgccgg aaaagcgaat cgattcttac
    73981 tcttggcaac tttcgggcgg actcagacaa agagcaatca tcgccatggc tattgtttgt
    74041 aaaccacggt tattgattgc tgatgaaccg actacggcta ttgatgtaac cacacaagct
    74101 caagttttga agctattaaa aaaactccag atggaaagaa atatgggaat tcttttcatt
    74161 acacatgatc tgggggtaat tgctcaaatg gcacaatatg ttgtcgtgat gtatttgggt
    74221 aggatcatgg aaaaaggacc ggttgattca atattccata atcccaaaca tccttatacg
    74281 caagcgttac tccgttcgat tccttcgatt caaaagacaa cgaaagttaa attgccgaca
    74341 atcagcggtt caattcctca tccgttcaat aaaccttccg gttgcccgtt tcaccctcgt
    74401 tgcaataact ggattcgcgg aaaatgtgaa aaattactcc ctccggagta tatagtagga
    74461 gaagggcaaa gcgccagttg tttcttatat gaaggatcgg aggaggtgac acatgaacga
    74521 gactaataat ccgtcgattc aaatgacgaa tacagagaga attcttgaag tgaaacattt
    74581 aaaaaaattt ttcccgatta cgaagggctt caggaagaaa catatcggag atgttcgggc
    74641 agttgatgat ctcaatttat atattaataa aggtgaaact ttaggattag tcggtgaaag
    74701 cggatgcgga aaaaccacgg cttctcgctg tatccttcga gcctatgaac cgacggcggg
    74761 agatattctt tatcgaacaa aaacaggtgt aacagtaaat ttgtcgcaga tgtccaataa
    74821 agaattacga ccgcttcgtg ccgaaatgca aatgattttt caagacccat atggctcttt
    74881 gaatccgcga atgaatatct ttgaaattgt cagcgaaccc atgctggtaa acggtataaa
    74941 gaatcgacga gagagagagg accgcgttgc ggaattgtta acaaaagtcg ggttacgtcc
    75001 ggaatatatg gttcgatatc cgcattcatt cagcggcggc caacgccagc ggatagggat
    75061 tgcacgtgct ttggcgttac agccgaattt ggttgtcgca gacgagccgg tatccgcatt
    75121 ggatgtttcg gttcaagcac aagttttaaa tcttctgatg gaacttttgg aagaaatgaa
    75181 tctgacctat ctgttcgttg ctcatgattt gtcggtcgta cgccatattt gtgaccgggt
    75241 tacggtgatg tatgtcggga aagtggttga gaccgcttcc acggaagcgt tatataatac
    75301 gcctcgccat ccttatacag aagcactaat ggcatccatt ccgattccgg atcctcgtct
    75361 gcaacaaaac agagaaggtc ttccgggcga ggttccgaat ccttcgaatc cgccgagcgg
    75421 ttgctatttt catccccgct gcagctattg catcgataaa tgtaaaaccg aaatgccgga
    75481 gctaaaagaa atagaaccgc aacattggac cgcatgccac cgagcggaag aactaaagtt
    75541 ggaaggattt cataaaaatg aatgattttc aagccgttgc catacaaaac agcaagatta
    75601 tttttccgga aaaagtcgaa gaaggaaaag ttctcttatt acgggatggc aaaattgccg
    75661 ggattgtcgg tcgggatgat tttaatcccg cagattattt gactatcgat gccgcgggga
    75721 agtataccgc tcccgggtta atcgatttgc atgtccatgg gtgttataac catactttca
    75781 atgaatctga tcccgaagca tttaatacga ttcttcgcaa tacacttcga tacggtataa
    75841 cgacgcttgt accgacactt gttgccgctc cgattccaaa tttgattcaa tcgctttgtt
    75901 ttatcgacaa gtggaaatca gaacaaaaac ccgggataac acaaataaca ggcgcttatc
    75961 tggaaagtcc ttatattgcc cctcaggcgg caggtgcgat tccggcatct gctttacgga
    76021 aaataacgga cgggtcaatc gaccaaatcc ttgaattaag ctatgccatt tcgattttta
    76081 tgattgctcc ggaattggaa ggcgcaacgg atgcaattcg caaaattaag caaaaaggaa
    76141 tcattgccgc catggggcac tcgatggcca tggagtcaga aataattccg gcgattgaag
    76201 ccggtgcatc ccatgtaacc catctatgga gtgccatgtc ttcggttgtc aggcaggggc
    76261 cttggcgaaa accgggttta ttggaagttg cgctggttta tcctcaactg acgacagaaa
    76321 ttattgcgga taatcggcat ttaccgccga cgttgatgaa gttagccgtc aaagcaaaag
    76381 gcgataaggt ttgtgccgta tcggatgctt taaatggcgc cggtctgccg gaaggatcac
    76441 attttttcgt cggtgaaaat gaatacgaag tggttgacgg agtaggaatg gtagccgatc
    76501 gaagttgttt tgcgggaagt acgacattat taggtcagga aattcctatt ttggtaaagg
    76561 aagtcggatt atcaattcct gaagccattc gtatggttac cgaaataccg gcaaaaattc
    76621 ttggaatcgc cgataaaaaa ggatcgatta gaaccggttt ggatgcggat attattttgc
    76681 tggactcaaa ttttaaaatt catgagatat ttcaacaagg aattctgatt caattttaaa
    76741 aattataaag aaatataaag gaaacaaaaa tggaaccaat atttacggat caatggggaa
    76801 agttaccggt caaagtttat gaaacaaatg agatgatggg agaagcagca gcactggatg
    76861 ctcgagaaat aatcaattct gcgattgctg aaaaaggttt tgcaaatatg atcattgcaa
    76921 ccggaaattc tcagctgaca ttcttagagt cactcagaga attacccgat attgattggg
    76981 gaaaagtaaa tgtatttcat atggatgaat atatcggttt gccggaagga catacagcca
    77041 gttttccata ttttttgcgg aaacactttt tggactatgt aaaagtaaaa aatttttacc
    77101 cggttcccgg aaatcccaaa actcttcgag aagatatgct cgcatatgaa aaattactaa
    77161 gggaaaatcc ggcagatctt tgtgcattgg gaatcggcga gaacggtcat atcgccttta
    77221 atgatccacc ttttgctgac tttgatgatc cggtctatat taaaaaagtc aggcttgacg
    77281 aaagatctcg gaaacaacaa gtaggggaag gtcattttca ttctctggat gaagttccga
    77341 tgtatgcctt aactttaacc ataccggctt tacgatcggc aaaacacatg ttatgcatgg
    77401 ttcccgagag cagaaaagcc gaagctgtat atcgaacgaa gacagaaccg atcaacgaaa
    77461 attgcccggc tactatctta aaagatactc cttggtgcac actttattta gacaaagatg
    77521 cggcggaaaa aattctaccg aaatagacag gatattactg ctttatgaag aaacaattat
    77581 ttattctcgg ggacagtatt tcgattgact acacaccggt cttaagagaa taccttggga
    77641 gtgaatggga agtgcttcgc aaaggggata tccctgcgcc ggaaatttca ggggaaatag
    77701 atcatgaaaa cggaaccgat tcgaatgtcg tttacgaata tctccgattg gtcttaccgt
    77761 ttattcaagc atccttaatt cttctgaatt ccggcctcca tgatatcaaa cggatgccga
    77821 atgaaacgga tgaatgccag gtatccatcg aaaggtatcg aaaaaatatt gaaaaaataa
    77881 ttcatctgat tcaatccgaa aaaaaacaag ccctgtgggt cacaagtaca ccggtagatg
    77941 atcagcagca tcgaaaatat gaaacttcat tctttcgacg aaatgatgac ttagttcgat
    78001 acaaccgaac agcaattgaa ataatgaaga aaaacaatat tcccgttttt gatttatata
    78061 actttacctc aagaataaat gctcctcttt accgagacca tattcatttt aataatgaaa
    78121 taatcaatct tcaagccgcc tttttatcgg gttgtattca aacatttttc agttatggaa
    78181 caagaaatga tgaggaatag atgaattctc gcgaacgatt aattacaaca atgaatttca
    78241 aggaaccgga tcgtgtgcct tttgatatgg gaagcacaca agttaccgga ataaatgaac
    78301 aggcatacag acgactgaga tccgcttatg gcttgccaga aaaagaagtt gtttattccg
    78361 atcaaattca gggactggct ttaccggatg acgattttat tgaggctctc ggaattgata
    78421 tccgcggact ttttccatta aacgctcata attggaatgt taaggttacg gatggcggaa
    78481 aatattggtt atatcttgac gaatggggaa taactcaccg taaacccaaa gagaatggct
    78541 tatatttttc gatctatcaa gaacctctga aaaatgagaa attatctccc gattccatta
    78601 aaaatcaccc ttggccgaat tttaaagacc ctcagcgaat cgccggatta aaggaactgg
    78661 ctgatttata ccactcaaaa aattacggcg tcgtgatgaa agatcctttt gcaggcattt
    78721 ttgaaatgtc tcagcggatt tgcggcatgg actatttgtt aatgttgatg gcaagtgaac
    78781 ctgatcttgc gggtctcctc ttcgataaaa tggttgaatt gaaaatcgat ttcttttcca
    78841 gtgcattacc tgttttggcc gattcagtgg atgttattgc attgatggat gattacggaa
    78901 ctcaggtttc tcaattgata tcaccccgaa tgtttcgcaa tcagattaaa cctcgttgga
    78961 aggaggtttt tgattgcatt aaacaatatg cacccaatgc caaacgcttc ttccattcgt
    79021 gcgggaacgt caggccgtta attccggatt ttatcgaaat cggcgtccaa atattaaatc
    79081 ctattcatgt taaagcaacc ggaatggatc cctatcaact caaaaaagat tatggagatc
    79141 aattagtatt ttggggcgga ggcgttgata cacagggaat tttgccgatg ggtacacctg
    79201 aggaagtcag agaagatgta aaaagaaatc ttgatgcttt aatgcccgga ggcggttatg
    79261 tttttaatac ggttcataac attcaagcgg acgtgccgac agaaaatctg gtcgcaatga
    79321 ttgaaacatt taaagaatat ggagtctatt aaatcaaaat gctaaaatcc gaccaatttc
    79381 ttccgcttga atttgttttc aatccgaact ggtggaataa aaccgccggg atttcctttg
    79441 atcgggattt ttatttgaat ccaaagaaga gagaagaaaa tgacgtgatg atgcgccgga
    79501 tattatttga aaagttcgga gaatttggtt tcggtgagga aaatcccaaa ccgcggccca
    79561 tcgccggatc gatgatggtt gccggtgggt ttgttattcc agcccttctc ggtgccgaaa
    79621 tccgtttttc ggcggcggag gctccgcaac cattacctca aattctgtca aaggatcaaa
    79681 taatgtcttt cgtggaaccc gatatattat ccaattggcc gatgaatgaa ttagcggacg
    79741 gatgggatcg gcaacaaaaa gaatacggtt atttattggg agatattaat accgacggca
    79801 ttttgaatgc cgcctatcat ttttacggcc ccgatctgtt tgccgatttg cttgaagatc
    79861 cggatgttcc tgttcgattc atgagaaaaa tcgcaaaaat aattattcag accgcaacct
    79921 atgtaaagaa tcgtaccggt actacttccg tttccgtaaa tcggatggca tgtcgtttat
    79981 ctccttcgcc ttttattcat gccaactgct ccgtccagat gatttctccg cgaacttatg
    80041 agaagaatct gttaccgatt gaacaggaca tggccaaatc catttccgat tacggcgttc
    80101 atcattgcgg agttaaaaca gaaatctatg cgaaatttta taaccaaatt cctgtttgtt
    80161 actgcgaaat cggatgggaa tcaaatccgg agaaatgtcg atcatttttg ccgaatgcat
    80221 ttctgaatct tcgcctgagt ccaattcgga tgctttcttg ttcttcttct gaaattaaaa
    80281 acgatgtgga agaattatta aattctgtag gcgatctgac caatgtggga gttagttgta
    80341 tcaatatgga tttcggtact cccgaggaaa atattatgac agtatcggag acagtcgaac
    80401 gatatagaat cttatggaaa aaacatcatg gataaattaa tacaaaattt ttttcaagca
    80461 tcttctcatc aagaagtttc aaaagttcct ttggcattaa ttgcagacag tccatggatt
    80521 cccggttttg cgggaattga tacgctggat tattaccttg atcagaaaaa atggttcgag
    80581 atcaatttaa atcttcggga gcgttttccc gaagttgtct gggttcccgg gttttggtgg
    80641 gaatatggca tggcaatcga ggcttctgca ttcggaagtc gaattatttt tcatcatgat
    80701 caaccgccgt ccatggaacc tatcagcacg gatttacagt tttggaaaaa taaaacaaaa
    80761 gtcagagatc ctgaaaagga tggcttgatg cctttggcct tgcgccaaat cgagcgttcg
    80821 gatcatgaat tacagtccta tggattgggt cagcatattg ccagttcgcg cggtatctta
    80881 acggttactt cctggtttat gggagtaaca accttaatgg aatctctctt gtccgcaccg
    80941 gatgatatca ttccgattct tgaagttgtg acggatacga ttattctgtg gttggaagct
    81001 caattaaaac ggatgaggga acccaaagga attcttttgc tcgatgattt gatcggaatg
    81061 atttccaaaa gagtttattt acgaatagtg gatcctctgt tccgaaaaat atgggatcat
    81121 tttgacggtt atgtgaaaat ctatcataat gacacctcat gcgaacattt gtatcctgtc
    81181 ctcgccgaaa ctggattcga tgtgttcaat ttttccgaca aaaccgatat cggaacagcc
    81241 cgtcaattga tgggcaaaca aatcgttttg atgggaaatg ttgcgccacg ggatatcgga
    81301 gctctcggta ctccggaaca ggtttaccaa gccgcctgga actgcttgga aaaaacaaac
    81361 ggagagggtc tgatactgtc ttttggtggc ggaatttctc ccggaactcc cgccgagaat
    81421 attgattcga tgataaaagc gttgaatgat tggtataaaa gataattttc cgaaaaatca
    81481 tctttttgga attattacga gaaaggttat cagatatgag taaaaaatgg aatttaccgg
    81541 aagatcgttt ttttgataac gaacctgctc aaaagaggat cgccctggaa atttatgaaa
    81601 gaataaagaa tttgccgatt gtcagtcctc acgggcatgt agatcctaaa ttattcgcaa
    81661 atgattcatc cggttttgga actcccagcg acttattggt catcccggac cattatattt
    81721 tcagactgtt ctactcacaa ggactgaaat tggaagattt tgggattcct tatcggaatg
    81781 gtttgcaaga tgcctccgaa aaagatcatc gaaaaatttg gcaacatttt gccgataatt
    81841 ttcacttgct tcgtacgaca gcttcttata catggcttgc ctatgagttt aagttcatct
    81901 tcgatattga tgagattccg gattccgaaa atgcgatgag aatctatgat aatctgacgg
    81961 aaaagctcag ttcaccggaa tttcaaccga gagaactata taagaaaatg aacattgaag
    82021 tgctatgtac aacagatgcg gcttcagatt cattgaaaac acatcaagaa ataaaagaat
    82081 ccggttggga tggaaagatt atcccgactt ttcgaccgga tgcggttacc aatatcagag
    82141 gagaaaactt ccgacaggaa atcgaaaaat tgtccgcagc cgctgatatc gatatccatg
    82201 attttgaatc ctatattcgg gcgcttgaaa aaaggcgagc cttttttaag gaaatgggcg
    82261 ctgtatcgac ggatcaggct gttattaccc cttatactca tgaacttccg caatctgaaa
    82321 cggataggat tttcacagct gctttaaatg gaaatgtttc cgattcggat gctacggcat
    82381 ttgaaggcca tatgttgatt gaaatggctc gcatgagctg caacgatcaa ttggttatgc
    82441 aattacatcc gggagtatta agaaaccata accgaatcat ttacgataaa ttcggtccgg
    82501 ataaaggttg cgatattccg attcaaaccg aatttacaaa gaatcttcat tcgatgctga
    82561 atcaatacgg aaataatccc aacttcacat tatgtttatt cactgtcgat gagaatactt
    82621 attcgcggga attagctccg ttagcgggtc attatccgtc cgtccgtctc ggaccgccat
    82681 ggtggtttaa tgacagttgg aatggaatga cacgattttt tgattccgta accgaaacag
    82741 ccggattata caatttgagc ggttttgtag acgatacccg agcgtttccg agcatccccg
    82801 caaggcacga tgtttggcgt cgggtcgttt ctaattggct ggcaaaaatg gtagtcagat
    82861 cgttcatcga tgtgaatgat gcctacgatt tcgcgttcct tttggcttat tctcaaccta
    82921 aaaatgttta taagctttaa tgattatttt aaggatgaaa tagaatgaaa tatagtctga
    82981 taaatacttc tgtaacaaca atcgatttgc aaaaaatttg gatgatcaga gatgccgaag
    83041 gaaatcgata tctgcaatct gatcgaccga taaaggcatt aaaaagtgaa aataatgaca
    83101 atatttattt attgtcgcct gaaaatgcaa agtggttgcg cagccaaatt ccttggttaa
    83161 acccccaacc tttgggatta aaaaccagcg tcgggacagg cgatcgctta gggatttcta
    83221 cccctggtca tattcgagct tttcaaggga cccaaatatc ccctgttttg gctcaacaat
    83281 ctgttcgaga attgattcga actcatcgaa ccgctcaaat ggtgcttgat gatgcaatgt
    83341 ggggcgtttt tgaagcggga tggaaccgtc cttggggtgc cgatgccgat cacttaaaga
    83401 cgatcgatga tatgagagaa tactacgaag caggatttac attctttaca atcgatccgg
    83461 gagaatatgt taaaaacctt aattcactgg ataatattcc tttgcaatca ttattaaaca
    83521 gtattgatta tgatctttta gctacttccg aatcagaatt gattaataac tatacgcatt
    83581 ttcccgattc gttagacttt ataacggctt ccgaacaaga tatcaaacgc gcttttataa
    83641 aatatggcgg agcaatccat catgttcaaa aaatgtattt tcaattaaaa agttggatgg
    83701 gcaataaacc gtttgacttc gaggtatcgg tcgatgagac ggatgaaccg acaacgattt
    83761 ttgaacatta tttcatcgct tgtgaattaa aaaggttggg catcgaatgg acctcgctgg
    83821 cacctcgttt tgtgggaaaa tttgaaaaag gtgtagatta tcagggggat ttgcatgaat
    83881 tcgaatctga atatcgaaag catgccgcaa ttcaaaaata tttcggaaat tataaaatca
    83941 gtatccattc gggaagcgac aaattcagca tctataaaat ttgcgctgtt aacagtgaat
    84001 ataaaactca cttgaaaacc gccggaacga gttatcttga agccttacgg attatcgcaa
    84061 ccgttgatcc ggaattattc agaaaaatct atgatctcag tatgaatcgt tatgaaatcg
    84121 ataaagcttc ctattatgta tcggcgcatc ctgaaaattt accgaaaata tcgagtttag
    84181 cggatgatcg actaccggat ttgttgaatc aatttgattc tcggcaagta cttcatgtct
    84241 gctttggggc ggtccttgat caatacggca cagatatcct tcgtgattta aacagaaatt
    84301 atgatttgta tctggatgat ttggaaaaac atttacgaaa gcatttgcag gattttattt
    84361 attccaaacc tgcggaagaa agttgaggaa taaatgacat tttcgaacga agttttcgga
    84421 aaaggaagtc ctgatccgaa agatttttta acggaatcgg atattaaaaa gattttgaca
    84481 gacttttttt cggcaaataa tttaagcggc aaaaaagtat tggtcattat tccggacagt
    84541 acgcgtacga tgccgcttcc gctgtttttt aaactgtttt acgagctcat acatgatgaa
    84601 gttaaaacgc ttgattttat gatcgcgctt ggaactcatc ctccgatgag cgatgaggca
    84661 atcgcaaatg aaatcggaat aagcatcgaa gaaatgaagg ggaaatatca ggatatttct
    84721 tttttcaatc atcattggga tgaccctaaa acttttattg aattaggtga atttcctaag
    84781 aatgaggtaa ttgaactgac aaagggaacc gttcaacggg caattccgat ccaaatcaat
    84841 aagatcatcc ttaattatga tgcactgatt gttttaggcc ccgtttttcc tcatgaagtc
    84901 atgggatttt caggaggaag taaatacttc ttcccgggta tcagcggtcc ggatctcacc
    84961 aatacaactc attggatcgg agcgttgcat acttgtataa aaacaatcgg gctaaagatt
    85021 acaccgcaac gccaattgat tgagcgggct gcaaatttga ttcccgttcc gaaactttac
    85081 tgttgtccgg tcgtaaaaaa agagggcatt tttggttttt acgccgggga gccttatcga
    85141 acatgggaaa aagcggtcga tttatcttcg caggtgcacg ttaaatatgt cgataagcct
    85201 ttcaaaaaag tcatttcggt tatgccccat ttatatgatg atatttggac ggcgggaaaa
    85261 gggatgtaca aactggaacc cgttgttgaa gatggcggtg aactgattat ttacgctccg
    85321 catattactg aaatttctta tactcacggt aaaacattag accggatcgg ctaccactgt
    85381 ttggattatt ttttgaagca atgggagaaa tttaaagatg agccatgggg aattttagcc
    85441 cattcaaccc atgttacggg attgggaact tatgagaacg gtatagaaaa gaaacggatc
    85501 aaagtttctt tagctaccgg cattccaaag gaaaggtgtg agcgagtgga tttaggttat
    85561 gtgaatccgc aagaattaaa tctgcaggaa tatgagcata aggaacatga aggaatattg
    85621 cttgtccctt atgccggcga gactttatat cggttaaaac aatgaacata ctttcaaaga
    85681 agatactaag aatgaaatga aaaaaataag gagatggata tgttaccgga tttcagtgta
    85741 aaaggaaaag ttgttgtgat tacaggcgga gggggagcca tttgcggaat gatgagcaaa
    85801 atgttcgggg aagccggcgc gaaagttgcg gtgatggaca tttctatgga tgccgcaaag
    85861 aaaattactg atgagataaa taacaaaggc ggggagtcaa tgccgttagt gtgcaatgtt
    85921 ctcgataaaa aaagtatcga agatgccgcc gctcaggtca tggggaaatg gggtagagtt
    85981 gatattttga ttaatggtgc cggcggaaat aagccgcaag ctaccaccaa taacgaaatg
    86041 tccttttttg acctgccggc agatgcgatt caatgggtat tcaatctaaa ctgccttggg
    86101 acaattatag ccagccaagt cttcgggaaa ataatggccg agcaaaaaga aggatgcatt
    86161 ttaaatatat ccagtatgaa tgcttttcgt cctctgacca gaattcccgc ttacagcgcc
    86221 gccaaagcag ctattaacaa ctttactcaa tggttagcag tcgatttggc aaagaatttt
    86281 tcgccgaata tcagagttaa cgccatagca cccggatttt ttgtggggaa tcaaaacaga
    86341 gatctcttaa ttgataagaa ttccggagat cttacccagc gcgggaaaac aattatcagt
    86401 ctgacaccga tgaatcgttt tggtgaattc gaagatcttt taggcgccac cctcttctta
    86461 gtttctcccg cctcttcatt tataacaggc gtggtattac cgattgacgg cggtttttct
    86521 tctttcagcg gtgtttaatg ggttcgaaaa taaaggaact atgaacatga gcgaattatc
    86581 aaaccaggcg aaagagttgc ttaatttcaa accaaaacat gaatttttcg tcggaattga
    86641 ttccgacggc tgtgtttttg attcaatgga aatcaagcat aaagaatgtt tttgcccgaa
    86701 tacaattcgt gtttggggtt tacagccgat ttcaaaatat gcccgtgaag cggtggaatt
    86761 tgtaaatctt tattcaaaat ggcgcggtat caatcgatgg cctgcattaa ttaaagtttt
    86821 tgaattgttg tctgaacgtc cggaagtgat tcgtcgggga gccgttatac cgaaaggaga
    86881 tgatatccgt gctttcatta atagcggctt gccattaagt gacgccgggt tgaaaaaata
    86941 tatggagact cacagcagcg aagaacttga tcgagcgctg gaatggacga catcggttaa
    87001 tcgaacagta aaagaaatag tccgcggctt gaaacctttt ccgtatgcaa aagaaagtct
    87061 gcaagccatt catcctttta cggatgcttt ttgtgtttcc gcgactccag cagatgcctt
    87121 ggaaagggaa tggaaagacg gcgaaattcg tgattatgtg gaaatgattt gtggtcaaga
    87181 aatcggaaca aaggaatata tcttaagact cggttctgcc ggaaaatacc catcggataa
    87241 aaaattgatg atcggtgacg ctcccggaga tcttgaagcg gcgcaagcgg taggtgctct
    87301 cttctatccg attaatccgg ggcatgaaga agaatcatgg gaaaaattct ataaagaagg
    87361 atataagcgt ttcctcgaag gaacttacag cggagaatat gaaaaacgct tgattgaaga
    87421 ttttgagaag ctgttgccgg aaatcccgcc ttggaagcga tgataaaaga ttaaacaaat
    87481 cccgaaataa ataacggaat gataggtagg caaataatgg aaaaagcaaa aatcggactg
    87541 atcggattag gtgtgatggg agccaatttg tccttaaaca ttgaacgaaa tggattccct
    87601 gttgctgttt acaatcggac tgtcgaaaaa acacataatt ttattaaagg agcggctgcc
    87661 ggtaaaaaga tttatggcgt agagactctg aaagatttgg tggatattct cgaacgtccg
    87721 agaaaaatta ttcttatggt gaaggccgga agccccgtag atgaaacgat tgaagctata
    87781 aaaccctatc ttgaaaaagg agatatcttg atcgacgcgg ggaactcatt cttccaagat
    87841 accgaacgtc ggagtgaaat gcttgcaaaa gacgggatca attttttcgg gatgggagtc
    87901 agcggcggag aagaaggtgc tttatggggc ccaagtatta tgcccggcgg gcagagagag
    87961 tgttgggaag aattgcgacc ggttctcaca gcaatagcag ccaaatataa agacggggaa
    88021 ccttgtgtgg cttacatcgg tcccagaggc agcgggcatt atgtaaaaat ggttcacaac
    88081 ggcattgaat atggagatat gcaattaata gccgagattt atgacctgct tcaccgcggc
    88141 ttaggtttag gtcaacagag attacaggaa atattcagta attggaattc cggagttttg
    88201 aaatcctatt tgattgaaat cacagcaaaa atattatcaa aaatcgatcc tgagacgggt
    88261 aaaccactgg tagatgttat tctggatgaa gcgcaacaaa aggggaccgg gaaatggaca
    88321 gctcagaatg ctttggatgt cggtgcaccg acgccaacat taacaagcgg tgtcgaagga
    88381 agaattttat caagtttgaa agaggaacgt gttgttgcga gtaaaattct gaaaggtccg
    88441 gatttaactt tttacggaga tcgggaagaa ataattcgag ctgcggaaaa tgcattgtat
    88501 tgttcgaaaa tcgcttccta tgcacaggga ttgggattac tgaaaatcgc cggaaaagaa
    88561 tatgactaca atctgaacct gggtgaaatt gctaaaattt ggcgagaagg atgcattatt
    88621 cgcgcggatt tcttaaatga tataaccggc gcttttaata aaaatccgtc tcttcaaaat
    88681 ctgatgctgg atgatttttt ccgatctgcc attgaaacaa gacaatcaga ttggagatta
    88741 attgcaaaaa cggctattga tctcggaatt ccgatgcttg cgacgaatgc atcattggca
    88801 tattatgaca gctatcgaag tgaaacgctt ccggcgaatt taacgcaggc acagcgagat
    88861 ttctttggtg cgcataccta tcgcagaatt gataaagaag gaagtttcca taccatatgg
    88921 gaggaataaa gtgaaaaaga atgtgattgt ttcacagtcc ggcggtccgt ctccggtcat
    88981 taatagcact ctgcgcggta ttgtggaaat gtgcagagat attccggata aattcggtga
    89041 agtttatgcg gggtaccatg ggatcgaggg tgtcctgaag gaagaattga ttaatttaag
    89101 cgcacaaaac gaggaggaaa ttgaatattt gagcgtgacg cctgccgccg gtggaattgg
    89161 gacatgccgg tataaactca gatcctcgca aaaggaagat tttgatcggg ttattgatgt
    89221 tttcagagca cacaacatcg gatatttttt ctatatcggc ggaaatgatt ctatggatac
    89281 agcaaacaag atatctcttt tggcaaatga aagagggctg gatttggttg tcgtcggcgg
    89341 tccgaaaaca attgataatg atgtgggcga cagtgaattt aaattaatcg atcatactcc
    89401 gggatatgga agtgtcgcca gatattgggc gttgaatatt caaaatgcca atgaagagaa
    89461 cgaaggttcc agtccggctg atccgatttt ggttttacag gcaatgggga ggaaaatagg
    89521 atttattccg gctgcagcca gattggcgga cccgaagaga gaaatgcctc tgcagattta
    89581 tatgacggaa tcaaaagtaa ctcttcccga attagccgat aacgttaatg aggaactgaa
    89641 aagaagcaaa agatgtattg tggtcatttc ggaaggattt gatgtcggag atattggcgc
    89701 cagacgcgat aatttcggac acatttcctt cgggtccagc caaattaccg ttacacaatt
    89761 ggtagtgaat tatctgaacg aacatggtct gaaatcaaaa ggcctggctc gaggagatat
    89821 gcccggaaca gatcagcgtc gcaatatgat ttatgcatct accgtggata tggaagaggc
    89881 ttataaattg ggtcaacaag caactttgat tgccgcatcg ggagaaacgg gatatatgag
    89941 tacgatttta cgaaatccgg gcccgattta taacgttcga tatgataagg tcccgttgga
    90001 tttagtcgcc aattccgaac gttttttccc cgaagcttgg attgctccaa accgaatgga
    90061 cgtgacggat gattttgtcc gctatgcccg cccattgatt ggggataatt ggcctgccat
    90121 tccactggtg aacggaattc agcgttttgc aagaattgaa cagattttcg tcccacaggt
    90181 acttccggaa tatgttccgg aagcatcgag gtaatcgaat cgctgaaatc cggattttga
    90241 aatcgaaaac gagatcaaac gataatcatg ataatattgc agatagtcag attaattgat
    90301 aaagaggaat tatatgtcag atttgttaaa tgaaatcaaa gaagcaatta ttgacgggtc
    90361 ggcaaagatt gtttccgaaa aagttaccgc cgcactggaa gaaggtttcg atccggaaca
    90421 gattttaaat gatgcaatga tcgccggtat ggcggaagtg ggacaacttt ttgaggatgg
    90481 agaatatttt gtgccggaaa tgttggtcgc tgcacgtgca atgactaccg gaatgcaaat
    90541 actgaagccc cgccttgttc aggctgatgt aaaagccgct ggaaaagttg cgatcggtac
    90601 agtccaaggt gatttacatg atatcggaaa gaatttagtc gggatgatgc ttgagggagc
    90661 ctcttttgaa attatcgatt taggcgctga cgtgcctccg gaaaaatttg tggaagccgt
    90721 tcaaaaagat catgttcaaa ttgtcgccat gtcggcttta ctgacgacga cgatgccttc
    90781 gatgaaaacg acaatcgaag cgcttgaagc ggctggtgtt cgtgatcagg ttaaagtaat
    90841 ggtcggcgga gctcctcttt cggatcaata cgcaagagaa atccatgcgg acggatatgc
    90901 accggatgct aacaaggctg tggcgttagc aaaaagtctg gtttcttaaa atgaagagat
    90961 aataaaaaga aaaggtgcag aaaaactgca ccttttcttt ttattatcag tcagttaact
    91021 ggtcaattac atcatgaacc aagagtaact aattgaagcc tgggttgtcg gagttgcttc
    91081 gacagcgggt gaagcttcgg caggagcagc ttctgaagga gctgcttcgc taggaacctc
    91141 ggtcggtttc ggtgtcgcag tagatgatgc acagccggca atgatactgg ctaacaaaac
    91201 aactaataaa acgatactta aagattttct catttaattc ctccacgatt tagtatataa
    91261 attatacata accttactta ttatagcctt tttattctat cacgcaacaa aattcatgat
    91321 attacatcaa ctgtctttga atttcccgta aaaaattctt tgtagatacg ggatgtgaag
    91381 taactccttc atataaatca gcgagatctt tggtacatat tccttcttcc aatgtgtcta
    91441 aggtcgcttt ttcaagtttt tttgcaaatt cttgaagctc cggaatatta tccatttctc
    91501 ctcgtttagc aagtgctccg gtccatgcaa aaatcatagc ggtcggattc gtagaggttt
    91561 cttcaccatt tagatagcga taatagtgac gggtaaccgt tccatgagcc gcttcaaatt
    91621 cgtaattccc atccggactg accagaacgc ttgtcatcat cgccaatgat ccaaaagcgg
    91681 tggaaatcat gtcgctcatt acatcgccgt cataattctt gcatgcccag ataaatccgc
    91741 cctcggatcg cataacccga gcaacagcat catcgataag tgtataaaaa taagtgatcc
    91801 cttcatcttc aaagttacgt cgataattat cctcatatat tttttcaaat ataactcgaa
    91861 aagttccgtc atattttttg gaaatcgtat cttttgcgga aaaccacaaa ttttgtttag
    91921 agtttaacgc atatcgaaaa caggaatgtg caaaagagga gatcgatgag tctttattat
    91981 agattccttg taaaaccccg gggctttcga aatcgtaaat cgtatcccgc atttcacttc
    92041 cgtcttcagc ggtaaataat aactccgctt ttcccttccc cggaattctg aattccgtcc
    92101 ctttatagac atcaccgtat gcatggcgag cgatagttat cggttttttc caagttcgaa
    92161 caaccggctt gatcgattcg attagaatgg gtgttcgaaa tacagttccg tccaaaatag
    92221 cccgaatagt cgcatttgga cttttccaca tttttttaag cttatattct tccatccttt
    92281 tttcattcgg agtaattgtg gcacatttca cacctacccc atattgttta atggcgtttg
    92341 cggcatcgac ggttatccgg tcatccgttt catcgcgggc cggcaaaccc aaatcataat
    92401 attcaacctt caagtcaaca taagggagaa tcaattcatc ctttatccat ttccaaatca
    92461 cccgagtcat ttcatcgccg tcgagttcaa cgatcggatt tttcatttgt atcttttcca
    92521 tttttcactc catgcttcgc agccgatttc ttttatttct tacgagcgta aaaattaatc
    92581 aagcttccgc tgataataat ttccctttct tcccgggtta acccttttag atgaagcagt
    92641 atatctttaa ttccggaacc ggtaataatt tttgctggaa tcgattcaat gcctcccgca
    92701 attgcttttc ggatacccgg aataaaaatc ttatctcccg gttgatattc gaacggttcc
    92761 tcgggatcga tcgtaaaagg aatgatcccc caattgatac aattgctgcg atatcgcttc
    92821 gtcgcatatt cgtatgcgat attggccaat cctcccagaa ctttttggct ggatgcagct
    92881 tgttctctgg cagatccgtc gccgggtttg tttgcaaaaa ttacggatcc gatggcggta
    92941 ttcaggttat ttgcgccgtt cggatggttt aatttatcca aaatttcttc aaatttcggt
    93001 aatttcgatc cttttcgtct ctctgattca agatcgcgaa tttttttggc tcggtttaca
    93061 tattccggaa ctcggcgaga aagtgtgaaa tcagctaatt ttaccggatt cgatcgataa
    93121 gaagaagtct ctcccgaagg aattaattca tcggttgttg tcaccggatc tctgatcact
    93181 gcggctaata cgagcagcag attttcagcc aacggataca tttccggcca aggcgtaata
    93241 ttcggactat atcttaattc aacatctttt tgcggttttt tatatccgca gtaaaccctt
    93301 tttcggtaaa tctcgctgtc gaaatgatag ggataggatt tatattcaat atccatatct
    93361 gcggccgaag taatataccc gctgtttgcg gcggttgccg caatcgaacg cgcatccatc
    93421 agagctacac cggtcacctg cccctgatcc ggtttcgatc cgtcacggtt gggaaaatta
    93481 cgggtagtat gacggatact gaacccgtta tttgccggaa tatctcccgc tccgaaacac
    93541 ggtccgcaaa acggcatttt tgtaaccacg cccgattttt gtaaacgtaa catgactccg
    93601 ttttcggtta gagcggcgtt aatcggcata cttgccggat agacgctcat cgaaaaataa
    93661 tcattcccga cgattccgtg atccaatatg tccgctgcgg cacataaatt atcgaacatt
    93721 ccgcccgcgc agccggcaat aatcgcttga tcagctaaaa tacgaccgtc atgtattttg
    93781 tcggtcaact tcagaataga tgctttgccg aatatttttt ccgattccgt ttctaccgaa
    93841 cggaggatat caccgggatt ttcaagtaac tcgcgaatgg tatatccgtt gctggggtgg
    93901 aaaggcaatg cgatcatcgg ttcaatcttc gacagatcga tatgaaccat cgaatcataa
    93961 taagcataat tctcaggatg aagcggtcga tacgcttcgg gtcttccgtg aagttcgaaa
    94021 taggttttta cagattcatc ggtttgccag atcgatgaca gacaagccgt ttcggttgtc
    94081 atgacatcaa tcccgtttct gaaatccatc ggtaaatact gaattccggg acctgcaaat
    94141 tccaatatct tatttttgac aaatccggta ggaaaaaccg cttttatcag agcgatcgca
    94201 acatcttgcg ggccgaccca aggattcggc ttattttcca aatataccaa tacaactttt
    94261 ggcgcatcga tatcatacgt tttttgaagc aattgtttta cgagctccgg tccgccttca
    94321 ccgatcccca aggttcctaa ggcaccatat cgagtatgac tatccgatcc caagatcatt
    94381 gagccgcaag ctgccatcat ttctctttcg aattggtgaa tgacagccag attggcagga
    94441 acatagattc ctccgtactt ttttgccgcg gaaagtccaa atacatgatc gtcttcatta
    94501 atggtgccac cgaccgcgca caaactgtta tgacaatttg tcagtgtata aggaaccgga
    94561 aattccttca ttccgctggc actcgctgat tgaataattc cgacataagt aatatcgtgg
    94621 gatgctaaag aatcaaaaac taactgcatt tgtgccggat tttttgaatg attgtgtttt
    94681 tccaatattt gataagcgat tgtatttcgg cggagttcag atatttcttt taatccggac
    94741 tctttcataa aatcggctaa tctttcatcg gttttctgaa cgaaaccata ggtttcggaa
    94801 taaatgatct ccttatcgat caattcaatc atgtaatttc tccttatatc atgaaaaatg
    94861 aatcataaac aaaaataatg cccggcttat ttttaaagct accagcagct ctttgatcaa
    94921 gcattattaa gcacttgcat atcaatggag gcaaatggtt ttttatcgga tactttgcag
    94981 atattcaaga acttcctttg cattcgtatt cgcattcgct ttttgaaaaa ttttaataat
    95041 tatcccgtct tcatcgataa taaatgtggt tcgaacgatt cccatggtaa ccttgccata
    95101 aaggttcttt tctttccata catcataagc ttttataaca tttaattcgg gatctgaaag
    95161 cagaagaaac ggaagcgcat ggctttcaat aaatttttga tgagactttt ctgaatcctt
    95221 gctgattccg atgatttcta cggctaaacg catatattct tcatacaaat ctctgaaacc
    95281 gatggcttgc cttgtacatc ctgcggaatt gtctttcggg taaaaatata gaacaacttt
    95341 tttaccgcga aaatcggaaa gagaatggat ttttcccatc tgatccggca gggtaaaatt
    95401 cggagccggc atcccaactt taagttctgt cataaaaccc cctctgattt ttttctttta
    95461 cggtcatttt gttttacccg atccaataaa tataatcgaa atgccttttt tatcaatatc
    95521 tttacatctt ttttgttcga acaatatcat taaatcataa aacttatctt gactgtatat
    95581 ataaaaaagg ctaaaatcag tgagctcttt gagtaaaata tctttaaaag ggtgaaggta
    95641 acaggtttaa tctgtgagag gcactcgaag gaagaatcat ggaaaaagtt gtaataaatg
    95701 ctgaaaagcg agatgttgtt ggcaagaaag tcggctcact gcgccgacag ggtttgattc
    95761 ccggtgtaat gtatgggcat aatgaacccc cgtttccaat cacaatgaat gcaagagaaa
    95821 tcgagcgtgt gattaataat ctgacgagct ccagtatcgt tacggttaag gtcaatggtg
    95881 agtcacacca agctttaatt cgtgaaaaac aaaaagatta tattcggaat caaatcattc
    95941 atattgattt tcaaatctta tctctaaaag aaaagatccg ttccaaaatt gaagttaaat
    96001 tggtcggagt tgctccggcg gtaaagaatt tcaacgggat tgtattacag gaacgtgagt
    96061 ttattgatgt cgaagctttg ccggctgatc ttccggagag aattacagtc gacatcagcg
    96121 gattagagaa tatcggagac ttgattcggg ttggtgattt ggatatttcc gatgccgtga
    96181 ccgtttttga tgatgtgaat gatgttattg tttcaatttc cggcgcaatg gctgaagaag
    96241 ctgttgaaga agaagtaacg acagcggaag gtacggagcc tgaagttgta gaaaaaggta
    96301 agaaagaatc ggaagaagag gaagaaaaga aatagcttcc ttttagatga tgtaaaaaaa
    96361 aagaccaatt cagaaaattg gtcttttttt actttctgtt ttggttttac accattaaat
    96421 cctcatcctt aatcaaaggc cttccgaaaa tcagaaatcc tgtgtgagcg atcattcgat
    96481 caaccggcct gaaccgttca gcttcggtct gataatatcg aattcctgtt tcacagactt
    96541 ctgtaaatcc aaactgattt gcattcattt gttctaaaag gaggattact tgattggcag
    96601 ttggcaacaa acatccaaaa aagcctcccg gttttaatgc atctctgact tgcgctaaat
    96661 aattataagg atcgggaata tcaaggaaaa gagcatcgac atttttttct gtaaatccta
    96721 accgaatatc ccgagttata aattcgactt gttcatccaa ggcaactctt ttcaggtttt
    96781 cttcggcaag cttttgaaaa tcagcccgac tgtcataaga atatactttc cccgtcgaac
    96841 cggccgcata ggcaaaagcg gttgttaaag cacccgagcc ggtaccggct tcaatgattc
    96901 tttttccggg accgattgcc agattaagca atataaaccc gacatctttc ggataaacaa
    96961 tttgtgtctc cctgggaatg tttttaatca tatctgttaa ggcaggttga atgacataga
    97021 acttattccc gttatgacta tgaataatac ttccccacgg tcgccctatg aaatcattgg
    97081 tgtaaataat tcccttatgg gtttgaattt cctgatcggc ttgaatttta attatgtgat
    97141 gaaagtgact gattccaaca atttcggcta tttcaccttc ttttataagt tgcttttcat
    97201 cccaactttc gttcattcga tttcactttg atccagtaat atttttttta ttaaacacat
    97261 aatccatctc tttccatgtt aatattatga aaattctatc aaattttgga gtttaatatg
    97321 aaaaagggta aattatttat tgttgcgatt gtttcgctta gcgttttatc ggtattatta
    97381 agcggcttta tttctgttaa accccaggat aaacaggagt ttaaagtcgg attggaatgt
    97441 aattatgctc cattcaattg gacacagctg gatgattcaa acggggcaat taaaattaaa
    97501 ggcggaggcg gttattgtaa cggatatgac gttgccatcg ccaaaataat cgccgatggc
    97561 ttaaatcgtg aactcgtatt ggtaaaaact gaatgggatg gtctgattcc ggctctaaat
    97621 gccggaaaaa ttgatgcggt tattgcggga atgtcaccga caaaagaacg caaagccagt
    97681 gtcgattttt cggatccgta ttatgagagt gaccttgtca ttgttgtcaa aaaagacagc
    97741 ccatatgcca atgcaaaaag tctccaggat tttagcggtg ccagaattac cgctcagttg
    97801 aatacattcc actacaccgt tattgatcaa attccgggtg tgaaaaaaca gaccgcaatg
    97861 gaatccttcc cggcgatgat cgtagcgctt tcatccggaa aaattgatgg ctatatttcc
    97921 gaacgtcccg gagcaatttc cgctatggaa tcaaatccgg atttaacatt tgtcgaattt
    97981 gaagagggaa aaggatttga aacctcgccg gaagatgttg caatcgctgt ggcattgaaa
    98041 aaagggaatc cggatttaga ggctataaat aaaattatct cgacaatcac aaaagaaaaa
    98101 cgccaggaaa tcatggataa tgccatcgca attcagccgc tttccggaga gtaaataagg
    98161 aacaggccgg aaatggatat aattccatcc atttctgatt atcatcaaca aaacaaccgg
    98221 aaaagtatat ttgcttgttc cggttgtttt tgtcggacac aatttaggaa tagattatgc
    98281 cttcaacttt tttagaatgg gttcgctttt ttgtcaatga atatggccta tattttcttc
    98341 aaggggcgtt aaccactctg tatatcgcct tgatcgggac aattgtcggt tttcttatcg
    98401 ggctcgctgt tgccgttgtg cggacaattc cgattgaaga gaatgattca ttcggtagaa
    98461 agatttttta taaagtggta cggtggattt tggttgccta tatcgaaatt ttccgtggca
    98521 ctccgatgat cgtccaatcc atggtcattt attatggatc agcggaatta tggggattgg
    98581 acatgtctcc gattgtagcc gctctattta ttgtttcgat caataccggt gcttatatgg
    98641 ctgaaattgc tcgcggcggc atcatttcga tcgataaggg tcaatatgaa gcagcgcttt
    98701 caatcggaat gaatcactgg cagacgatgg aaaaaatcat cctgccgcaa gcgatacgaa
    98761 atattttgcc gtcgatcggg aatgaatttg ttgtcaacat taaagacacg tctgttctga
    98821 atgtaatttc ggtcaccgaa cttttcttta tgtcaaaatc agccgcagga acttatatgc
    98881 gttattttga agtctttttt attacctgtg taatttattt tgttctcact ttttccgtaa
    98941 ccagaatctt gttagccgtg gaaaagaaac tcgaaggacc ggaaacctat atcatccatg
    99001 gatctcagac tgttccggaa gctgtaatta aaggtgaaat ccgatgagtg agtttattat
    99061 ctctgttaat catctgcaaa agaatttcgg cgagaatcaa gtcttaaaag acattgattt
    99121 ttatgtaaaa aaaggagaaa taatcagtat tatcggttcc tccggttcgg gaaaatccac
    99181 tttgctccgt tgtattaatt tgctggaaaa acccacttcc ggcacaatct catttcatgg
    99241 caaagatatc caatccaaag gtttttcgct ctccgaatat cgttcaaaag tcggaatggt
    99301 tttccaaacc tttaatttat ttaataatct gacggttctc gaaaattgtg ttgtcggcca
    99361 aatgagtgtc agtaagattg atcgaaaaac tgccgaagaa aaagcattga gaattttagc
    99421 ctccgtcggt atggcgcaat atattgacgc aaagcccaaa caaatatccg gcggacaaaa
    99481 acaacgcgtg gcaatcgctc gtgctttggc aatggaccca gaagttcttt tattcgatga
    99541 acccaccagt gcgctcgatc ctgaaatggt cggggaagtg ctcaatgtaa ttcgtgattt
    99601 ggcaatttcc ggattgacga tgctgattgt tacccatgaa atgaattttg caaaagatgt
    99661 ttccagtcgg gtaattttca tggatgacgg tgtgatcgtt gaagattcat caccggaaaa
    99721 aatcttttcg gatcccgaaa atgatcgaac tcgtgctttt ctgaaacgta ttttaaatcc
    99781 cgagaaagat cccggcctgc aataagtaat cagcggcaat aactgaaagc tgaaccatga
    99841 agaaagagaa attcatagcg tttccgttgt tcttaatctt agcgattgct gctatttggg
    99901 taaccctttt caaaccgatt ccgcgtcaga tcgggctgat ttttaggcaa gaaatctata
    99961 ttctgccttt gatattttta ttggctttac tcccgtcgat tcagaaatcc gccgcctggc
   100021 aagcggtatg ttatgcggtc ctatttactg tttgtcttct gcctttatcg ggtctgtgga
   100081 attccggcat ttcggatcaa tacatattcg gcagttcaat accctggtcg gatgccttta
   100141 ctcatcatct gaatacttta cgattcctat atgggcaaac catgggacaa tcgacggctg
   100201 ttcgtcccct ttctgtttct ttttatagtt ttatcctata cataacaaat aataatttta
   100261 ttatcctgat gagcagttta gccgttttat cggctttcgt aatgctgatt aatttccgat
   100321 taatcggcat tcatatcgga cctgttgccg cagctttctt tttcacgaat gcattctttt
   100381 ttataagaaa gtttttaggt atttatatga cggaacctta tgcatttatc atagggttac
   100441 tcgcttgtta ttttttattg aacgggattt tcaagaaaca aaacggacag attcttctcg
   100501 gattttgcct tttttcatta gccatgaatg cccgcccggg tcccatgttt acaattccgc
   100561 tcatagggct ttggtatttt tttatttttc tgaaaggtca tcatcgccgg ataatatgtg
   100621 cgaccgtcgc tttcatagca ttattaagcg gattcgtcat aaatcaatgg aatgtaaacc
   100681 gggtctatcg atccgataaa gtacccaacc ttcaggcggc acaaatcatt tatggattat
   100741 gcctcggagg gaaagattcc tattatgtga tgggactccc ggaaataaat gaattaaaca
   100801 aatccgagaa tatctataaa gacgttttcg gactttgttc gaatgtattg aaagaacatc
   100861 ctgaaaatat cggggtctca ttcaatgcaa tttggaatac gctcttgttc gattcgaatc
   100921 gcggttcatt ttcttacttc gacggtactg aaaataaaat gctgattgat ttgatacgaa
   100981 tgggattgat ggtattgtgg tcgatcggaa tatttttaat atttcggcag agaaaacagc
   101041 catttggttc atttatgatt gccatggttc tgggcttgat attatcgcaa tttgtggttg
   101101 cgccctatac tacctttcga atgagatatc atgccgctac aatttgggtt cccggtttaa
   101161 tcatcggtat tttcccgcag tatgtcatca atcgatttgc atggaaaaat ttaaaaagag
   101221 agtccgaaag taccccggta accggttcga cttcactggc tttggctctg tccggttttt
   101281 ttatcctcat cgctttgatt gcgccggaat taatcggtac gcggacttta aaaccacctg
   101341 ccaaacctga aatatcttgt aagaaaggtg aaacaacgct ttatacaaaa attgatcagg
   101401 gcagctattt ttatatggaa aacaaatccg agcttcaaca agaacactat ccctatttca
   101461 ggctgccctt tgttcgacaa cattttcatg ataccgcttc ggttgagatg ttcgacttta
   101521 ccgaccgaat tgatcagcct acggcgatta ttcgcggcat tgatttatcc gattggaaag
   101581 atgcattgat tttttcaccg cttccgctcg taaaggatcg tatcggatac gttgtatttt
   101641 gcggcaacta tcttgacccg cccattcttc ggcaggatcg attttttatc ccgtcttcgg
   101701 catatttttt acaatgaaat aggaatattc agaacaaatg acaaaattca ttttattacg
   101761 gcatggacaa acagattgga atattcaaag actttatcaa ggtcaggaag atatcccttt
   101821 gaatgagaca gggaaacgac aagcagaaga agctgctgaa accttaaaaa atgtcccgat
   101881 cgatattgcc tattgcagtg atctgcagcg ggcggttgaa acagcgaaaa ttgttttgcg
   101941 ataccatccg tcggttcccc ttatgattga taagagaatc cgcgagagaa gctttggcga
   102001 atacgaaggg aaaccttatc aaaaggattt gatgaatcct accgattcaa atgaaataaa
   102061 caaaaagagg gattctttga atttcaaatt cccgcagggt gaatctttgt tggatgttta
   102121 tgaacgggca aatagttttt acaatgatat tttacaaaaa cacccgaatg agacagtttt
   102181 gatcgtctca cacggcactt ttctttccat tctctccgct attatttatc aggatgaatt
   102241 agctgaaaga aaaaagtata tcttcaacaa tgccgaaccg gtttttatcg acccgacata
   102301 aaacaattaa tttttcactt cctcccgcca tccccaaata aaatagggag cgtccgggcc
   102361 cgtattaaaa agcacgtcca agatagaaac ctgatgctga aaagggtcat gcagctgttg
   102421 atactgcggg tatcccgaat aatctttcca aattagttct attccggctt cccgataatc
   102481 tgagctgata atataggact gggctgccgg accggattca taagaattcg caccggtatt
   102541 tttaaccaat gataagagtt tttggtgacc actgccggcg gatgaatatg aagtgctccg
   102601 atccaatttt gttgaaatat gtaaaaaatc tgtcgcaatt tttcgaataa ggaattggtt
   102661 taattcggat aaactattcc aaaccgtatc taaataaacg aaatccaaaa aatccctata
   102721 ttgtcgaaaa tatggtgcct ttgcgtaatt cgtcaggatc gcttggtaat gctgcatttg
   102781 ccaccgcccc cgaacgattt caatttcatc aatacgcttt tcggttgtcc catataccgg
   102841 aacggttatc catttcaatc cggtggccgt ataaatttta ttgcgattac gccaatcatt
   102901 tttggtatac tgaacttcat cataaaacaa gaataaatct acatcatgga taatatcaaa
   102961 atagcctttc catggcaaat aattggattg caataccgct aatttcatct cggtttaatt
   103021 acgtttaaaa cgataaaaag acatgataga ttgtgtaaca gtcgcaatat cttctgaaga
   103081 cagtccgtaa tacataggca atcggagtaa acgttcactg atcgaagtcg taaatcggtc
   103141 ttctccttgg aaacgaccaa atcgctttcc ggcaggtgag ctgtgcagcg gaatataatg
   103201 aaaaacagtt tctatttttg attctttcaa atattgaatt aattttgttc tttctgacaa
   103261 actattcgtt ttaatgtaaa aaaggtgggc attatgaacg cattcgggcg gtatcgaagg
   103321 aagttctata aatccggcat cggcaagcgg ttttaattgt tcataatatt cattccaact
   103381 tcccaatcga ttttgattta ttttttcggc gttcaataac tggggataaa gataggctgc
   103441 attgagctcg ctgggtaaaa aggaagaccc tttgtccacc caagtatatt tatctacctg
   103501 acctcgccaa aatttgctgc gatttgttcc tttttctcga atgatctccg ccttctcgat
   103561 aaaagattca tccctgatta agatcgctcc gccttcaccc atactgtaat tctttgtttc
   103621 atgaaaactg tagcatccaa agtctccgat cgatcccaat gcttttcctt cgtaggaaga
   103681 catgacgcct tgcgcggcat cttcgatgat catcagatga tacttttcgg cgatttttac
   103741 aatcgtgttc atttcacaac cgatacctgc ataatgaacc ggaacgatcg cctttgttcg
   103801 atttgtgatc gcattttcaa tcagagattc atcgatattt aatgtgtccg gacgaatatc
   103861 cacaaaaaca atttttgcac cccgtaatac aaaggcattt gcggttgata caaaagtgaa
   103921 agaaggcatg atgatttcat ctccttcccg gatatcacat aataaggcag ccatctcgag
   103981 ggcatcggaa caggaagtag ttaacagaac tttttttgct cctgtttttt cttccagcca
   104041 agaactgcat aaatgggtaa attctccgtc gcctgatatt ttatggtttt gaatagcttt
   104101 ttcaatatat ttctcttctt caccgatata aggaggaaca ttgaatgaaa tcatcgatat
   104161 ccgttttctt ctttcttcgt aataaattag gtaatcgtct ccctaatttt atatcaaatt
   104221 gttgaataaa actttattta tgaataatcc cgggctcgaa tataatttat agaaataacg
   104281 tttttttatt aaaaacgaat tactgaacca aggacgatcg cagatcatga cagattccga
   104341 tttaaaacgt ttatatccaa tatcagcaac gatcaattca aaaaatcatt tcacaatcgc
   104401 cggttcggat ctttccgatc tggctgaatc atatcgtaca cctttctata tttatgaccg
   104461 ggataccatt cacgaaaaca tacatcgaat ccaatcggca atggctgatt tttattccgg
   104521 tccgggaact atcgcgtatg cttccaaagc ttatttatca cgaaaatttg cctcaaaaat
   104581 agcgaccgag gaagtcgatt tagatgttgt aagtctgacg gaacttcgat ttgcactggc
   104641 tgcgggtttc agtccggacc gaattcattt tcatggaaac aataaatcta tcgaagagat
   104701 agaaattgcc gttaaccaaa atatccatgc gattgttgtc gataatatgg atgaattgct
   104761 gttgacagaa aggatcgcaa attcgaaaaa aaaggttgct caaatttggc tgaggattac
   104821 tccggacatc cgcgttgcca ctcatcctca tatcgaaact tccgctgcga ccagcaaatt
   104881 tgggtttcat atcataaatg gtgatgcggc aaacgcgatc gaatatgctg taaattcttc
   104941 ggctctttgt cttacggggt tgcattgcca tttagggtct caactctttg atccgcttcc
   105001 ctatcaaatc gcaatagaga aaatgttcga tttagcgata aattgtcact acattccgct
   105061 tgaattgagc cccggaggag gttggggtgt tccgtatgtt gaagacgatc cggatagcaa
   105121 tccggaacaa tgggtaaaag ccatctcgaa gacaattacg gaaaaatgca actgttatca
   105181 aattcgtttg ccgaaattga ttttagaatc ggggcgtttc attgtcgccc gctcgggagt
   105241 agctatctat caaataggaa ctcagaaaaa atccgtaaac ggtgagagga tcatagcggt
   105301 tgacggcggg atgtccgata atcccagaaa tgcgctgtac caggcaaaat atacggcgac
   105361 cccggttgat caatcggttc atagtcgtaa gaatccatgc aaagttgtcg gaaaatattg
   105421 tgaaagcggg gatgttttaa ttgataacgt tatgctccct gatcttaact acggcgactt
   105481 attaatgatt ccggtagcgg gtgcatatca attaagtatg gcttcaaatt ataatttggc
   105541 gccccgtccc gttgttttat ggttagaacc agaagggaaa gtcgaagttc tccaaaaaag
   105601 agaaaatctt gagcaaaaca gttggtggga ttgatgtaat tttaattgta tgacaattac
   105661 attaccaaaa ttatttatca ctggattaga ataaaagtat acaaaatccc gacatctttc
   105721 ttgcggttca gatagatgta aagcgggaat cgataaataa aatcataatt ttctgatttg
   105781 atataaggag aaataatgaa aaaacataat tttaatgccg gtcctgcaat ccttcctgat
   105841 tacacttatg aaaaatgcat agaagcaata aaggattttg ataatacagg gctttctatt
   105901 ctttccgttt ctcaccgttc aaaagaattt gacgcagtaa tggacaatgc agttgcagcg
   105961 acgaaacgcc ttcttgatat tccgtcaggt tactccgtaa tctttttggg cggcggggct
   106021 tcgactcaat tttatactgt tccttacaat ttcttgggca aaaaagccgc ttatcttgat
   106081 acgggtactt gggcgatggg tggaatcaaa gaagctaaat tattcggcga agtggatatc
   106141 gtagcttctt ccaaggataa aaattattct tatattccca aaaattatac ggtccccgaa
   106201 gatgcggatt attttgagat ttgcagcaat aataccatcg aagggactga aattcgtgag
   106261 gattttgatt gcaaagtccc gttggtcgcc gatatgtcat ccgatatctt tagtcgacct
   106321 gtagacgttt caaaatacag tctgattttc ggcggtgcac agaagaattt tgctcccgcc
   106381 ggcgttacga ttgtaattat caaagattca tttatggata aagttgttcg accggttccg
   106441 acgatggcaa attataaagt acatgtcgag aacaattcca tgaaaaatac tcctccggtc
   106501 ttcccgattt ttgccggatt aaaaacgatg gaatggatgg aacaaagcgg cggtgtcaag
   106561 gaaatggaaa aacgggcgat tgctcgctct tctaagattt acgatgcttt ggattccagt
   106621 aaattgttta aagctaccgt tgaggaccca caggatcgct ctcggatgaa tatttgcttt
   106681 gttctgaaac cggaatatag tgaacttgaa accgcatttc ttgaccttgc aaaacaacgc
   106741 ggtttggtcg gtatcaaagg gcatcgttct gtgggaggtt ttcgtgcatc ctgttacaac
   106801 gctttaccga tggaaagcgt cgatgctctg attgatacaa tccatgaatt tgaaaaagca
   106861 cactaattca acaagaaagg gccatctcat gaagaaagta ttgattgcga cagaaaagcc
   106921 tttttccaaa gcagctgttg aaaaaattaa aagcattttg gaaagcggtg acattacggt
   106981 gaaattgctc gagaaatata cagatgtaaa ccaattatat gaagcggttg cggatgtaaa
   107041 cggactcatt gtccgaagcg atgagatcac caaagatgtt attgatcatg cccccaattt
   107101 ggagatcatt gttcgtgccg gtgccggtta cgacaatatt gatcttcaag cagcgaagga
   107161 aagaggtatc gttgtcgaaa acactccggg acaaaactcc aatgcggttg ccgaacttgt
   107221 tgcgggcatg atgattatga atgccagaaa tttttatgac ggctcaaccg gaacagaatt
   107281 gaaaggcaaa aaactcggaa ttcaaggctt cggtgccgta ggaaaatgtt tggcaagaat
   107341 agccaatgga ttgggaatgt acgtccattt ttatgatatt ttcgtcccat tcgaagcagg
   107401 ctatgaatat aatgtattcc cggtagaaga tataaaatac ctttataaaa cgtgtcaata
   107461 tgtttctctc catatccctg cccttccctc tactattaaa tcaatcaatt atgatttaat
   107521 gtcacttatg ccgcagggag ctgttttgat caacagcgca cgggcagaaa ttatagatga
   107581 agacgattta cttaaaatgt ttgaaaccag acctgatttc cattatcttg ccgatgtcgc
   107641 accgtcaaat aaagctgtta tcgaagaaaa attccctggt agattcttct tcactccgaa
   107701 aaaatgcggt gcccagacat ctgaagcgaa taataacgcc ggtacagccg ccgcaaatca
   107761 gatcgtcagc ttcttcttaa ccggagataa aaaatatcaa gtaaataaat aggtagttta
   107821 aaattaaaaa acagcctgct ttttttgtga acgcaggctg tttttttctc ggaatagtat
   107881 aatcgtttcc gatatgaaag gaatttttca atggaaccta ttaccatcgg aaacatagtc
   107941 agggtcaatc acagttttat cgacgaagat agcgccataa atctgaaagg gcatgaaggg
   108001 gttgttcgaa tgatattttc acctgaagaa aatgaccctg acgaaaccct atatttaatt
   108061 gaatggacgg aaaaaacatt aaaacaactc ccggatgact attatattaa actgtttaca
   108121 gatgaaaaag aatggtcgta taagatcgtc cctgaacaca ttcttttaaa agttgaaggg
   108181 gaatataatg ccgatgctgt agagtgggag aaaaatgcca tcggagcgcg cttattttgg
   108241 caatatttcg ggagatccgg taaattaatt caatcgttgt ttccgaatga caaggaaaaa
   108301 acagaccgtt ctccttatga tacatggcta aagtatctga atcaaaattt gcgcttccct
   108361 ttcgaagcta cgatttttta tgaatacgaa ccggatgacg gaattttaca accttccgat
   108421 tcggtaaatg ttcagggatt ggcaggctgg gattatcctc tcggcatatt ttgcgaagtt
   108481 cagtttaagg ggaaatattt tatcctcccg ctgcaggata tcggaatgac caatgaactg
   108541 tcaaaaaatg ccagaatact gaacgcatat gaagcatggc ttttatgtcg agcttgaaca
   108601 gctcaaattt ttttatcggg gatataccgg ttcggggaaa aacaatactc gcccccatgg
   108661 atggatatag cgacagtccc ttccgtcaaa ttaccaggaa acttggatcc gccgccagta
   108721 ttacggaatt cgttaacacg atggaggttg tctctcgtaa tacgaaatat gaagaaagat
   108781 gttcttttca accgtccgaa cgcccgatag gaattcagct ttttgacaat aatcccgacc
   108841 ggattattcg aggggcggag atcatagtta ataagtggaa tcccgattgg attgatatca
   108901 atatgggctg ttcaatcagc catgttgcta accggggtgc cggtgtcggt ttattaaaag
   108961 atccccattt ggttgcagaa ataatttcgg gattaactgg tcgactttcg gtcccgataa
   109021 cggcaaaaat tcgtttggga tgggataatg aaagcattaa cttcttggag atcggtaaaa
   109081 tcttacaaga taacggcgcg gcaatggtta cattgcacgc acgtaccggt aaacaattgt
   109141 tttcaggaaa tccgaattgg gatgcaattg ctttattaaa acaaacactt caaattccgg
   109201 tcatcggcaa cggagatatc aaatccgttt ccgagataaa atcgatgctt gattatacag
   109261 gttgcgatgc tgttatgatc ggacgtgccg ctctgcaaaa tccatggatt tttgccggtt
   109321 atgatgcgga tgaggttcct ttgttcttat ttcaagaaat ttgtttggaa catttgtctc
   109381 tgatgaaaag tttttacggt gatactcggg gttgcattct gtttcggaag tatgccaaaa
   109441 aatatttatc ccataccggt ttggaaaaac acctgattcg ggatttactg actacttcgg
   109501 atcctgactt atttactgaa aagtttaccg atttgattat ttctaccgct caacttattt
   109561 agttttttca attcaaagaa aatataaaaa tggaaaattg tcttcaaaat ctttttaccg
   109621 aatcggaatg ggaactttta tgcgatcatt gcggagtttg ctgcttttat aaaattgaag
   109681 atgaggacac ttcggaaatt ttttataccg aagtagtatg tccttattta aatctcgaat
   109741 ccggattatg tgaggtttat cctgatcggt ttcaaaaaat gtcgacttgc ataaaaatat
   109801 ctccggataa tattcaagaa atatataatt ggctgcctcg acattgtgca tatcgatgtc
   109861 tatacgaaaa aagaagcgtt ccttgttggc atcctttata ccaaaatccg gataagcatt
   109921 ccgaattaat ccgcaaaatt gaatccgtca taaaatttcc ctttaatgat gggctcagtc
   109981 aaaatgagat ccaaaaaatc aaacaaaacg caattcgagc tgattattcc atagattttg
   110041 aagagcaatt aattcatcat ttgattaagg acagcgaaat ttaaatcgaa tcttattttg
   110101 ggtgatcaaa tagcggaata atttttttgg cacgaaatct tgtccaaaga gtttgcgcga
   110161 gtaccgcaat attcattccg atcatgatta catagattcc ttcccatttt tgggtcaaaa
   110221 ttccgatata tagaatcgat atcataaaaa ttaaataaat taacagggac tccaaaaatc
   110281 cgctggtctt ttttccggcg agtaaaatcg cctgataaaa attcaaatag gtattgacaa
   110341 acggaaccag aaagatcata ggaagggcct ttttcgcaag atcggataat tccggtgtga
   110401 gggaagaaag accttcaaac caaaaacgac ctaaaggtgt aacaattaaa gcggcataaa
   110461 acaatgtact gaaaattgct atataaaatg taaatctacg atcagcttta tagaatcctt
   110521 cacgattgat aacagataag gtcgtctcat tacatgcact gccaaaagat ttaataatat
   110581 ttaaaagccc ggataaaaca ggccaaaccg ctaatgaagc aatcggatta atcattcgac
   110641 tcatcgccgc actgcccaac gacatccaac ccagattaat tatttgcgtc aaaatcaacg
   110701 gtattacaaa tcgaatcagt tctccccaag atatcagctc ggataattct ccttcttcaa
   110761 aatctctgtc ggcggttttt cttcctttta atccgcaaaa aagtccttca gcgattacgc
   110821 cggaagataa agccgcacct gctgtcatcg ttccgctgac aaaatcttgt aaccggtaac
   110881 cgatcagcag gaccccgatt agaaaagaca ttcgaatgat ggttccaatc gttacttgtt
   110941 tcgaatgacc gttccgaatc aatattccct gataaaagcg tctgtatccg atcgcccagg
   111001 tccacggaat catcatgatc atttcgacac gggcataagg taataattct ttcggagctc
   111061 ccattaaatt tccaaccacc aaataaaaca aaggtgtaca actgattgaa gcatgaatga
   111121 tggttaatcc tgcgcaaagg atcatcatga atttatatat acgacgataa ttctttttgt
   111181 ctttactgag agccaacgaa accgaaagca tcataataat aggtgcttca ataattaatg
   111241 cgatcggata gactacgcta tgtgcggcta agtttatttc cggctgcggc attcgactga
   111301 caatcgccga aacaaaggga atatcgacag ccataaaaag ccaactgatt gccaacggcc
   111361 aaaaaatatt gaagatttgt tttatctttt ctttatcgct tgacattcca ttctatttta
   111421 tcctcggatg ccgttttttc ttcgagtcca tgaatccaat tttttgatct ttttattctt
   111481 atgttttata ttcgttaaaa tattaataga tttgatgatg aatctattaa atcagttcaa
   111541 attttatttg aataatccga tataaattgc tctgaggaaa atgtctttac cgaaaaatca
   111601 aatcggtcga atttccgttt ttctttcgga aattttttca aggatgatct taccgttgga
   111661 taattcgttc agtttttcct caatttcagg aaatcgaaat gattcaactt tggcagatat
   111721 ggtaatcttc tccgcaaaaa tatgatcaat caataaaaca ttttccgtgt taaaaagatt
   111781 ttttatcagc tcaaacagat gataatcgat atcaaatcga aaagaatcca ttaatacttt
   111841 ttcagcacgg gggacgattg ataatacctt tttggcggca ccgccataag catgaaccaa
   111901 tcctccgata ccgagtttcg ttcctccgaa ataacgggta acaaccatag caatatcacc
   111961 cattccgctt cccgtgagaa cggtcaaaat cggccttccc gccgaaccgg cgggttcacc
   112021 gtcatcatta cagtaacaaa cggttgaaga atgatggcct attacatatg caggtacatg
   112081 gtgcgtagca tccggatagg tcttgcgtat tttcaaaata aaatcttttg cttcttcaac
   112141 cgaaaatacg ggcgcacagg tggctataaa ttttgaattg ataaccgtta tttcagcttt
   112201 ggtttcgacc gcagggacga aaagccgttt tgaactgttc ggatattcat catctaccgt
   112261 gctttttttc atggagataa attaattgag tctcattttt tatggagttg gtcccatgct
   112321 ccaagccagg gagttcctaa tcggtgatta tcggatatcg taacatcatt tagtgattga
   112381 ctcaattcga tgagatcggg aattattttg ttgtaatttt ctgtcagtac attcgggcgg
   112441 atccgttccg ccaacaaacg cgcccactcc cattccattc gaaaatgatc agcttttaac
   112501 caatatccgc gcttttccca tgcttccgcc gattcttcaa ccgtttcagt gattcgttcc
   112561 agacacaaca aaataaaagc aatcagatct ctggaagttt cgttcggttc cgattgcttc
   112621 atcaactctc ggatcgccag aaccagagcc ttttttaatc tgtttctttc agtacttgca
   112681 cttccggtac gaattactct gctcataaaa tatctttata ccccatgttt ttattttctg
   112741 ggatcgaatt atacagtata agtattgatc actccatata agaatatttt gtctctataa
   112801 cctccgcgta ttctcttcaa tttcacaaaa tcaaaatgac cggccgattt cctgataaaa
   112861 tggaacaggt taaaacatat gtcccgaaaa attcgctatg gtctttttgc aattgccttt
   112921 tctctccttt ttttatcggt tgttcttttg attatttcaa gggatatatt ttattctcaa
   112981 tgcaatattc ttcctgaaaa ttcaacagct gggccgttga atatcggagg tttgacaatc
   113041 tcagaggcta tggaagaaat aaaaacgctt tcatcgattc cgctcgaatt aaaatatcaa
   113101 gaaagtttga ttcaggttaa tccggaacag ttttctttca aaattgacat cgaaaaagca
   113161 caatccgaat tggaatcaaa aacatccgag atgtgttctt caaaaacatt tttaaacagt
   113221 ttgttcaggg aacaaaaaag tgaaccaatc tcgatagcac tggaatccaa cgcagatgaa
   113281 gaaaaaatcg agtcattttt gcgaaacgag ataagcgctc gatatgatca accggcagcg
   113341 gcaaaatatc cggatcttaa cggttccggt ttttattcgg ggcatcccgg ttatcaattg
   113401 gatatcaaaa aagccgttaa aatgattcaa gaagccttaa cttcatttga tcatcagacg
   113461 attgatttac cgattatcag tattcccgag ccgccatctt ctttaacaaa tttggaaatt
   113521 ttattatcct cggttgtaga tcaaaatcaa gacccggatc aaatcaccga aatatactta
   113581 ttagacccca agaccggcga agaatttgat tttgcccgcc agaataaatc aaatttaatt
   113641 ccggaaattg cttttaccgc tgccagtacc ataaaaatcc cgattatgat atccgctttt
   113701 aaacacttgg atgagaaacc gagtgccgtt gtttcccgac agttggaatt gatgataacc
   113761 gaatcaaaaa atgaacagac agattggctc ttggaaaacg taatcggcgg agagacggcc
   113821 ccgttagaag ttacaaaaga tatcagggcg ctcgggtttc aaaatacatt tcttgccggg
   113881 tatttttatt taggtgcgcc tttgcttgat cgaatagaaa ctccggcaaa tacacgaaca
   113941 gatatcgacc taaagccgga tatctataac caaacaacgc ccaaagatat cggtttgttg
   114001 ctgtatttga tttatcaatg tgatttatac ggtaagggga agcttacgga ggtttttccg
   114061 aacgaaatca cgcaaagcga atgcagtgat atgatgatat tgctcaagaa caatcatttg
   114121 ccatacctga tatcggcggg gataccggac ggtatcccga tagcacataa gcacggatgg
   114181 atagaggaat ccgatggact cctccatacc atgagcaatg tggctgcggt ctacagtccg
   114241 ggcggtgatt atattctaag tatctatacc taccatccgc aaaatttgat ttttgaaaaa
   114301 ggaaatattt tgttctctcg tatttcttcg gcagtatatg gattttttaa tccgcaacaa
   114361 aacgacactg tcaattaacc aagcttccgt tttacagata atctttcaaa ttatgttcga
   114421 ccgcataagc agccgcttcg gctcggttgc tcgtgcctaa tttggataag atactgctga
   114481 cataatttcg aacagttcct tctcctaaga ataaagactt tgctatttcc cgatttgtct
   114541 ttccttccga aacaagaatc aacacatgtt tttcctgttg acttaatatc gcaaatgccg
   114601 atgcttcttc ttcttttacc gctcggcgaa cctcctgaaa gacacgttgt gtcacaagcg
   114661 gatccaaaag agcatctcct ctggctacag attcaagaga tcgaaccaaa tcttcactgc
   114721 tgatttgttt taacaggtat ccagaagctc cggctcttat tgcagaaaaa agcatctcat
   114781 cttcggcata agatgtcagc atgacaactt tgacatccgg atatttttgc gtaatttctt
   114841 cacaggcgtc aataccggat tgcccgggaa gccgaatatc cattaaaaca atatccggtc
   114901 gcaattttcc ggtgatttcg atcgcttctt ttgcattgct cgcttccccg accacctcga
   114961 atcgcgatcc ctgttcaaga agagatttaa gaccaagtct gacaacttca tgatcatcta
   115021 cgagaacaat gcgtatttta ttcatctgtt ggaactccta cggcaataaa taatttttgc
   115081 ttttttaatc ttaaaaaaac aatgacatgt cataatttta atatgtcata ttattcttat
   115141 caacctgaca agtttcattg tacactaaaa aactatgcaa ggcaatgcgt ttaaagtagc
   115201 aattctcggt ggcggtccgg caggaattgc tgctgcatta atgatacgtc atatatccga
   115261 ttatgatgtt tatctatttg atgcaaaccc gaaaatcggc agaaaattat cggtaacggg
   115321 gagcggacga tgcaacctga ccaatcttga attagatccg gagcgatatt attcaactca
   115381 gccagcggat ctcagtcctg ttttctctcg ttggtcaccg gctgatttac ggttgttcct
   115441 tgatcggatc ggtattccca ccattgcgac cgacgatggg tgggtttatc ctcagtcttt
   115501 ttctgccgca aatgtcgttc agattctaaa tggcaatcta acggaaaata atatccatat
   115561 aattgaaaat acaaaagttt tatcgatcac gctttacgaa aatcaatttc ggttaaccgt
   115621 agataacagg aaagattctc tggttttcga ccgaatctta attgcgacgg gaagtaaagc
   115681 ttttcctcaa ttacgagcag acacgggtat tgtgtcggag ttgaaaaatt tcggaattca
   115741 tgtcgctcct tttttcccgg cattatcccc ggttttgttg gaaaatgaac ctcataaact
   115801 tctatccggc attcgcttgg atgtaaacct gaaacttttc cgggaaagca ggcaaataga
   115861 tgaatccttc gggaatatca tttttacaga ctttggaatg aacggcccag gctgtatgaa
   115921 tctcagtcac cgaattcagt tgcttcttga aaatcatccg tatttcaagg tcgattttat
   115981 acctcaagaa aaatcaaaaa taattgaggg gatttttaat agcaagcaag atcaaaatta
   116041 tccttatcgt gatatttttc tctccatatt accgatgaaa ttagtcgatt tcttttttaa
   116101 tgaatggaat atagctaccg atacaaaatg ttccgaaatt aatcatcaat cgttcggttc
   116161 tcatatagat caattaagga attttaaaat gcaaattaaa gggacaaaag gatttaaaga
   116221 gtcccaggct tgttccggag gagtactctt atcagaaatc gatctccgga caatggaatc
   116281 cttaaaaatt ccggggattt attttgccgg agaaatattg gatgtggtcg gtccttgcgg
   116341 agggtacaac ctccattggg cattttccag cggattaata gcgggagaag cgatgggtaa
   116401 accttaacta caagtcggat aatttttctc tttccctttt ctgagtcttc ccgcgttctt
   116461 cggtatccaa aatcttcttt cgaattctaa aattggtcgg agtaacttct aacaattcgt
   116521 cttcagccaa atattctata gcttcatcca aactcatctg ccgcacggcg ttcaaacgaa
   116581 cttcgatatc tttattggat tggcgcatgt tggtcagatg ctttttcttg caaacattca
   116641 cagtcaaatc cgtcacgcgc atggattcgc cgacaaccat tccttcataa acttccacgc
   116701 ccggaccgat gaataaaaca cctctctctt cggcgttttt taaaccgtaa gtcgtcgtca
   116761 gaccgtcttc ccatgcaacg atggagtttg tattacgcga agcaatcgga ccggcatagg
   116821 gggcgtattc cagaaaagac gaattaataa cacccattcc gtgagtggct gtaagaaact
   116881 gataacggaa tccgagtaat ccccgagtcg gaacgatata atgcaaatgg accgaactgt
   116941 ccggattatc cgtaatttcc tgaatcctgc ccttccttga ccctaacatt tccactaccg
   117001 tcccgaccgt atcagggctg gtatcgatgt aaacttcttc aaacggttcc aataattcac
   117061 cgttggatcc ttcgatcata attacttcgg gtctggaaac ttgaaattca tatccttctc
   117121 tgcgcattgt ttcgataaga atagctaaat gaagctcgcc ccgtcccgag acaacaaaat
   117181 tatcggtagt atctgtctct tcgactctta atgcaacatt gtttcgcaat tccgaaaaaa
   117241 gacgttcccg taactttctg gacgtactga attttccttc tctcccggcg aaaggagagg
   117301 aatttacacc gaatgtcatt tttacggtcg gttcttccac ggaaataaca ggtaatgctt
   117361 ccggataatt cggatcggat agggtatccc cgatggaaat ttcattaagc cccgcgatag
   117421 aaataatttc acctgcggag gcttcctgcg tttcaacttt attcaatccg ctgtaagtat
   117481 agagatatct ggcattttcc ggtacgatag taccgtccgg ctttattctg gataaattca
   117541 taccggcttt taattttccg cgatgaattc ttccgatgac ccctaatccg cgatattcgt
   117601 cataaaatat attactgacc accatttgaa acggctggtc gatttccact tccggcggat
   117661 tgatttttct taaaattgtt tggaacagcg gctgtaaatt ttcggacaat tccggcgtat
   117721 atccggcttt tccttttatt ccgtccgcgt aaatgatcgg aaaatcggct tgctcatccg
   117781 tggctcccaa atcaataaac aaatcaaata ttttgtttaa tgttcgctcg gggtcggcat
   117841 cttttcgatc cattttatta atcacaacaa ttactctcaa acccatagca agtgcttttt
   117901 ttaaaacgaa acgagtttga gccatcggtc cttctgcggc atccactaac aacagtgcac
   117961 cgtcgaccat attcatgacc cgttcgactt ctcctccgaa atccgcatgg ccgggtgtat
   118021 cgacaatatt tatctttacc atttcaccgg tttcggggtc cggtatcgag atggcggtat
   118081 tctttgacag aatggtaatt ccgcgctctc gctcgaggtc attggaatcc atcactcttt
   118141 cttcaacctg ttgattggca cgaaatactt tcgcttgttt cagcattgca tcgacaagtg
   118201 tggtttttcc atgatcgaca tgagcgataa tcgcgatatt tcgaaggtca tttctaattg
   118261 ttttcatttt tttataattc aaatcagagt ttattgctct gtctcttttc ctaaatagat
   118321 taaaggtaca ctcacaagcg taacgagata ttttattacg acattcccga taaatatctc
   118381 ccatacagcc ggtaacggga tcattccata aaaagcgata aacgcaaaaa gcagagagtc
   118441 aacaggaaca ctcactccgt tggaaatcaa aacgcgtgcc cacagatgtt ttcttgtaat
   118501 tttttcaacc caccaactgt aaatttccgt atcgattaat tcggaacaaa gttccgcaat
   118561 aatagaagcg atagttattc gccacaccgg cgttaaaact ttgatccaat tttcacttcc
   118621 accagcggat aaatcaaccg gaagaacgga aacaaagaaa aaatatccgg ccataaataa
   118681 atttattccc gctgcggaat aaataagcgt ttttgtatgt tccttcccta atattttatg
   118741 ggcaatgtct ctgagtgtaa aagaaatcgg gtaaataaaa gtaccggcat ccaaagccaa
   118801 tccgaatatc gaaacaattt gcaagctgct gatatcggcg atcatttgaa cgccgatgta
   118861 agccgcaatc gcaataattg ccgctttgtt aaatgagaat ggcgtcttta ttttatccgg
   118921 ttccatacca acggtggatt ataatgagtt ttgggtttgc cggcaatttc cggtcaaaaa
   118981 ataatgatga ccgatcgata tgaagttata tttttctggt aaactgaaga caaaataaga
   119041 aaatccctga aaaggataat gaacggatga aaaaaaaggt tttaatacta accgcggatg
   119101 ccggattcgg gcacagaagt gcagcaaatg caattgcaag cgcattgtcg aaagaaacaa
   119161 aatttgagat tgaaacaagg atcgagaatt tattggattc acccaagaca ccggcacctc
   119221 tccgaaatac tcaaacggga tacgataaaa ttgttacgga atctccgaaa ttctatgaat
   119281 tcagttatga aagcagcgat aaacgccttc cgacaaatgt ggcaaagatc ggtctctctg
   119341 cgatgctgta tggtccattt tcaaaaacaa tgaaagaatt caatccggat gtccttgtaa
   119401 atacttatcc attgtatcaa ggccccgctc aaacttggta ccttctcaat gaggatgcaa
   119461 aaaaagcgat cagtaaatca aaaaaaacag atgatgattc caaatcatcc tcgaggcata
   119521 aacacatacc gatgataaca attattacgg atcttgtttc cgttcatttg atctggatga
   119581 gcacattgcc cgatatctat tgtgtcgcta cccatgaaat gaaagagcaa atcgccggtt
   119641 tcggagtcga tcgagagcgg attctatgca ccggcattcc cgttaatccg gtttttgccg
   119701 aggaaaaaag atcaaaaagg gaattaagag agcaattggg gtggaatccg gaaaaaacta
   119761 ccatcattgc gatcggaagc aagcgtgtct caaatatgat ggattatttg gatcttatca
   119821 accatagcgg attcaatatc caattgataa tggtcgccgg tggagatgaa acactctatc
   119881 aggagatgca aaatacgact tggcacgttc cggcaaaaat ctataatttt acaaaagagt
   119941 tacctgtgat gatgcttgca tcggatattg ttgtaacgaa agcggggggg cttgttacca
   120001 gcgaatcgct tgcagcctcc ttgccgatga ttttaatcga tgttttgccg ggtcaggaag
   120061 agggaaatgc tttatacatc caagagaaca atgcaggtgt ccatgcaaaa aaaccgatcg
   120121 acattttgca tacgctgacg cactggttcg aaaatgacgg atatggatta aggaaattta
   120181 cggaaaacgc cagaaaaatc ggtaagccaa ggtcaagtct ggatatagct gaccttattg
   120241 tagagaaatt atcagaatca taggagaaaa aatgaaaaag aatttatttt ggattttcgt
   120301 gtcggtttta tttttttccg ttttcttcaa cggaaccaaa gtcgaagcga tttcgtttga
   120361 taaggttgtt tatctgatca acggatcttt aggtgataac tctttttatg attccggtca
   120421 agccggtatc gatcaactga aaaaagatta tggagtagaa gcccgtacca tagaatgcgg
   120481 ttttgatgcc gggaaatacg aaccctcgtt ggacgctgcc gttcattacg cagatgtaat
   120541 ttttgtcata tcatacggat atgaggatgt tcttaaagag tatgccgaca agtatccgga
   120601 taaaatattc gtcaatctgg atacggtcgt cgaaaatgat gaacatacca taacatcggt
   120661 ggactatatc gaagaggaaa gtgcttttct tgccggtatt gtcgctgcgt tggttacaac
   120721 cagtcaggaa atcccgaatg tcaatccgca aaaaataatc ggagccgtcg gtggagatac
   120781 ggatcctgtc gttcaatcat ttatttttgc ctatgaaaac ggggcaaaat atatcgatcc
   120841 ggaagtgaag aatgagacca aatatctcgg cgattgggaa gatacatcca aagcgaaaca
   120901 ggcaactttg caactttacg atatgggcgc ggatattgtc tttcagattg ccgccgcagc
   120961 cggaatgggt gtgcttcagg catccggaga acgcgggtta tattcgattg gcgtcgacac
   121021 caaccaaaac gctatcgttc ccggccatgt agtcgcttcg gatattaagg atgtgggaaa
   121081 gtcgattttg aatgtttata aaacaatcaa agacggaact tacaagcccg gagaagttct
   121141 ttcatacggc ttggaatccg gtacggttga cgtggtattt gaaaacaata atgatgtttt
   121201 gccgaaatcg attatcgaca aggtttccga agtaaaacaa atgattatca acggagatct
   121261 taaagtcgaa cgttatgttg ccgagtagtt caaaaaatta ataaataatg acactccgaa
   121321 ttcggagtgt cattatatta tcccgcagta catctgtagt ttctgttctt tttgaacggg
   121381 catctatttt caattctttc tgtgagaaag gaaaataatc ttgagcgaaa tgaccgaatc
   121441 ggtacgtcgt gatgataatt taacctcatt gattgaaatc aaccatttgg tgaaaatttt
   121501 tcctccgaac gttgtagctt taaatgatgt gagtgtggat tttcaggaag gcgaaattca
   121561 tgcgatcgtg ggagaaaacg gcgccggaaa atctacttta atgaaaatat tatacggaat
   121621 gataccggcg gattcgggtg aaatcttgta tagagggaaa ccgatccgtt tcgcaaatcc
   121681 gggagaagcg atccgttcca aaatcggtat ggttcatcag gaaattttgt taattccgca
   121741 atataccgtt tgggaaaatg tgatattggg gatggaaccg ctcggtaaat ttaatcgtat
   121801 ccatcgtgat ctcgcacgaa aacaagttca agatcgtatc gatgaatttc aatttaattt
   121861 ggatccggat gcgaaagttg aagatctttc tgtcgctgcc agacaaaaag tcgaaatact
   121921 gaaactcctc tatcgggacg tcactttttt gattttggat gaacccaccg ctgtattgac
   121981 tcctcaggaa attccgcaat tgttcgatga acttcgcagg cttcgaaata atggccacac
   122041 gatcgctttt atttcccatc atttggatga agttctcgaa ctcagcgatc ggatttctgt
   122101 tctgcgcaaa gggaaaaaag tggctacgat tccagcgagt caaacatcgg aatccttact
   122161 ggcggaaatg atggtgggac gcgaagtcgt ttttgagtct attaaagaaa cagtccgtcc
   122221 gggaaaatta atatttcgtt tgcaaaatat ccgtttgcaa tctcccaata aaaagaaact
   122281 tttggatgat gtcaatatcg aggttaaagc cggcgaaatc gtcggtatag ccggagtgga
   122341 aggaaacggc cagctcgaat tggttgaagt cataacggga ttggcgacgc cggatcaggg
   122401 acagcttctg ataaacggag tgaatttgac aaaggcttct attttggaac gcagaaaata
   122461 tatggcattc gttccgcaag atcgggcctt gatgggctca tgtctgaatg catccatcaa
   122521 agaaaatctt attatgaccc actataaact gaaccccgaa tttacgcgtc acggacatat
   122581 tttaaatgat aaaaaatcga cggaatttgc ttatgaaatc cgggaaaaat tcgatatcca
   122641 actccattct atcgaagatg aaatgaaatc attatcgggt ggaaaccaac agaaagtcat
   122701 cttaggaagg gaactgctcc tttccgcacc ctttattttg cttgaccaac cgactcgcgg
   122761 cctcgatgtc ggttcgattg aatatgttca tcaacagata ataaggatga gcaaggagaa
   122821 tcgagcaata ttactccttt catcggatct cgaagaattg tttagaataa cagaccgcat
   122881 tctggtcttg caccggggac aaattgtcgc aaacttgatt acaaatgaaa ccaatattaa
   122941 cgaagtcgga tatctaatgt tggaaggagc cgctcatggc gcataatctt cggaatattc
   123001 taatcaatct tgccggtatt ctgatcgcat tcttattggg agctgtcgtc atgatctttc
   123061 agggatataa ccccttggaa acatatgaag ctcttttcgg ttattctctc ggaaatacct
   123121 attcctttat gacagttttg aaaaattcgg tccctctgat tctgaccgga ttatccgctt
   123181 cggttgcctt tgcttccggt ccggtaaatc tcggacagcc cgggcaattg ttgatgggtg
   123241 ccttatttgc aacggtcggc ggtctttatg tcgatcttcc ttcagtatta atgattccgt
   123301 ttttgttggt gtcagccatg gtaggaggag cactttggtc ggggattgcg ggtttgctca
   123361 aacggttgtt caacatggac gaatatatta cgacattgat gatgaatatg attgcggact
   123421 tttttacata ttgggcgatc acctatcctt tttttgaaaa aaatgccaat caccctcaaa
   123481 cgccgcgaat cacaaaaaac ggctggatgc ctgaaatcgg tgactttaat acgaattttc
   123541 ttgtgacatt aatcgttttc gtcattatat ggtttatttt ttatcggact accaacggat
   123601 atgaatggcg catttccggt aaaaacagcc tttttgcacg ccttggcggt gtcgagatcg
   123661 aaaaaaacta tatgcacgta atgctattaa ccggtgcgtt ggccgggttg gccggcggtt
   123721 tgattattat ggcagggcct catcgattta tcaaaggatt gggagccaat tacgcttggg
   123781 atggaattat ggtttccatg gtcgccaata acggtattgt cgcaacattg atatacggta
   123841 tcttcttctc cattttgcaa acgggtgcgc tcggaatgga attaatcaca caagtaccga
   123901 gtgaattaat tacggttttg caagcggtca ttgttttggt tgttgtcgcc agcagagggt
   123961 attttacatt gctgctttca aaaatgatga ccaaacgaaa actgaaagag aggtctgcat
   124021 gacgattttt atcggattga ttaatggaat gatttcagcg gcaacgccaa tcctcttatc
   124081 ggctttggga ggcgcgctga cttattatgc cgggatattt aatattgcaa tggaagggat
   124141 gatgctcagc ggcgcttttt ttgccgtatt gggatcatat tattttggga attggctcat
   124201 cggcatgctc ttcggtatct tgggtgcttt gctgattgcc ggtatcttta ttctgttttc
   124261 agtcgtcttg aaagtggatg aatttgtaac aggcgtcggt ctaaatatgt tttccgtcgg
   124321 attgacaacc tatctgcttc gtcagatttt taaagtgaaa ggtgctttta ccgatccggg
   124381 aatttactct gttccgaaaa tcagactccc tttcatagaa aaaataccgt ttgtcggaga
   124441 tattatcagt gatcaaaatc ttattgtctg gatagcattc atagcaaccc tattggtaaa
   124501 ttattttgta tttaaaactc agtttgggct tcgtttaaga gccgcaggat ataatcggat
   124561 ctgtttggat accagcggag tttcttccaa caagattaaa acggtttcat tattattatg
   124621 tgccgtttta tgcggtttcg cggggactta cctttctttg ggatatgtaa cattattcag
   124681 cgaaaatatg tctgcgggtc gcggatggat ttcactcgct gccgttatat tagtttcggg
   124741 caatccgatc ggaatcgctt tggtttctct ccttttcggt cttgccgacg gattgggttt
   124801 gtatttgcag catattttcc catcgcaatt cacttcgatg ttgccgtata ttgcgacgct
   124861 tgcggcacta tttttctatt ctgttaataa taagaaaaaa atggaggtta agttatgaaa
   124921 gcttgggaaa tggaacgcga aatacgcgat aacgccgtcc ctaaggacag aagaaagaat
   124981 gaatctcact ggtataaatc ggatatgacg attccctatg gggatgagtt gatcaagaaa
   125041 ttttggaaat ccaatgtacc gggttctttg gcacccgaaa ttccttatct tgaaatgacc
   125101 caagcattaa gtaatcaagg gtatgatgta tctgcggcag aaaatttaat cccagaaggg
   125161 attcaattat tcgaaatcgg tgatatcgat aatttacgag ttttaaccgc aaaattatta
   125221 aatgccttaa ataatgcacc gaaaatcgct gaccaccctt atcatcaaca tgaatatcca
   125281 atgtcttggg aagaaatcta tcgggcgatg cctatcagca gccaagcttc ttcatctcgc
   125341 cccgaatgga aaaatacatt taaaaataaa atatatcaag gatggatagg tcagttagcc
   125401 ggaggctcat tcgggacagc gatcgaaggg tatatcggat cacaaatcga attaatttac
   125461 ggagaagttg attcctatat aacaactccg gaaacgacaa atgatgatgt ggtctatgaa
   125521 cttgctttct tggatgcttt tgcaaagaac ggcagatatg taacttcaca acaagtcgga
   125581 cttgaatgga ttaaacaaat tcaattcggt tggtcagcgg aatggattgc gattcgaaat
   125641 ctgaatatgg gaattttccc gcccgaatcc ggccgatttt taaactttta ttcggattgg
   125701 atcggcgctc aaatgcgagg aatggtttgc gggatgttag cccctgcctg gccggtcgaa
   125761 gcggcacgat tggcattcac cgatgcggtc gtttcccatt caaacaacgg tgtctatgga
   125821 gaaattgctg cagcggttat gacagctttg tcatttgaaa tggaaaatcc gcgagacatc
   125881 attcaagaga ctcttaaata ccttccggca aaaagcgagt atgttgaaaa atatctgatc
   125941 gttttggata ttcttagaac ggaagaagac ccgaatattg cacgcggaag aattgaaccc
   126001 cattttatcc gctataactg gattcattct tatccgaata tggcggctga tttatacgct
   126061 ctgtggtacg ggaacggaga tttttccgaa tccatgaaac tgcttgccaa atcaggaaat
   126121 gacgtggatt gcaatgccgg gctggtcgga aatatcttgg gaattatccg ggatgttccc
   126181 cgaaaatggt ctgacccgat cggcgattta ctcgaaacat atatcaaagg gaaagaacga
   126241 ttatcgatcc gacacctcgc cgaaaaaaca tccgaattgg cacaacaata aatcgaccaa
   126301 aagatttagt cttataataa tttcgcgaga atatttattt aaagattgaa caaaaagcgg
   126361 atttattttc agatactcag aaaggagatc aaattatgcc gatttatgaa tattactgtc
   126421 aagattgtaa tacaaaattt gagctaatcc gggatattgc taaagcgaat gatcccgcaa
   126481 aatgtcagaa atgcggcagc aataacacga aacgattaat ttcaaaattt tacagccaag
   126541 gttcatcgga aaattcctcc tcgtcaggtt gttcatcctg ctccggcgga aattgttcat
   126601 cctgtggtca ttgattgata tgatggcaaa atcacttcaa tcaaaattgg tcttgatttc
   126661 cggcggttca agcggcatcg gtctcgcaac cgctaaaagg atggcaagag aaggtgcctc
   126721 ttttttaatt ttggcacgag atgaagtaaa gttgacaaat gccagagaat tgatccttcg
   126781 agaagcggtt tcgaatgatc agttcgttga aacattatcc gtcgacatga gaaattatga
   126841 ggaattaaga aataaattgg ttcccatact ggatcgttta ggaacgccgg attatgcgat
   126901 gcattccgcc ggagttgttt atcccgctct ttttgaggat ataacgatcg agcagtttca
   126961 ttggatgatg gatacaaatt attttgccgc agtcaacctt tttcaaatta tcattccgag
   127021 gatgaaagcc agaaggtcgg gacatattat tgcgatgggc tccgcttcct cttttattgc
   127081 catctgggga tattcggctt atagcggatc aaaatacgca attcgcggat tgatggaaac
   127141 acttcgttgt gaattaaagc cgtttaatat ccaaacttca atcgtttttc caccggatac
   127201 ggataccccg caacttgcat acgaaaatca gtttaagccg gcgataacca aagaggttag
   127261 cgggacaatt aaaccgcttt cagcggattt cgtagccgat aaaatttatc ggggagttat
   127321 gaaaaaccaa taccaaatta tcccggaccg agatactcaa ttaacttata aagccgcaca
   127381 tattctcgga accggaattt ttaaagtttt ggacatggtt acagacaaag caattaaaaa
   127441 atatgggttg gaatcaaaaa acatttatac cgaggagaat aaatgaaaaa aaaagacttt
   127501 ttatcgatcg ctgatttctc agaatccgaa ttaattcata tattagacgt cgcacaagca
   127561 ttgaaaaagg aattaaaaga gggaggaaac aagccgattt taaaaggaaa aagtatggcg
   127621 ttggtttttc aaaagccatc tttaagaacc cgagtcagtt tcgaaatggg gatgcatcaa
   127681 ttgggcggct atgcatttta tctttcaccg gatgaggtcg gcctcgggaa acgagaggcg
   127741 gtaaaagatg tggcgatggt tttagcggga tatgtcgacg caattatggc acgggtattc
   127801 caacaccaac atttaattga attagccgaa ttttccggta ttccggttat taatgggttg
   127861 tccgattata accatccttg ccaggcaatg gccgatgcgt taaccataat tgaagaattc
   127921 ggcaaattga aagggatcaa tatcgcattt atcggcgacg gaaataacgt ggccgtttca
   127981 ttaatggata tagcgacaaa attaggagca aacttttcaa tcgctaatcc caagggatat
   128041 gatatgcctg aagaggcaat ttcagccgca aaggaattcg ctaaactaag cggaagcagg
   128101 cttgtttttt ccaatgatcc atatgaaatt gtgaaaaatg cacaggttat ctataccgac
   128161 acttggatca gtatggggca ggaatcggag gccgaagaaa gaaagaaagc ctttattcct
   128221 tatcaagtca atcaaaaatt agtagatgcc gcgcctaaag atgtcattgt gatgcattgt
   128281 ttaccttctc accgcggaca ggaaatcacc gatgaagttc aagatggccc tcattcccgt
   128341 gttattccgc aagctcacaa tcgtttgcat gcccaaaaag ggattatggc ttatttactt
   128401 gctgatttat aacattattt tcatcgaaaa aagagctgca ctcttcagca aataagtgca
   128461 gctctttttt gtttccattt attcagaaga taatttttac gatagactca aaaaattgca
   128521 ataaaagcat taatatgatg gggctgaaat caaacattcc gatcgtaggc ataattcgcc
   128581 gaatgggatc caagaattga ttcatcaatt gatctaattt cattcgaaat tcattataag
   128641 ggggtacaaa ccaggaaatt accacataga ttaataacaa tatcgacatg atatttacaa
   128701 aagtcgaaac taaaattaca aaaaaattac tcatttattt cctcttattt gaaatattat
   128761 atcctggggc ctgtcaatgc agtcccgatc cggacaaatg ttgccccttc ttctatcgca
   128821 atcggataat caatgctggt tcccattgaa agatgatgca acgaatagcc ataattatca
   128881 ttcaattttt caagcatata ccgcaatcgt acaaaataag gacgactctc ttcgggattt
   128941 tccgaaatcg gaggaagtgc catcaatccg tcaaccttta gttgagagag agttcggatg
   129001 acttctaagt cttttaataa ttgatcggac aggtttccgt tttcagagct ccaaccggat
   129061 ttgctgtctt cgttccctat gtttatttcc agaaaaaccg gcatcgtttt attaatcatt
   129121 tttaaaattt gatttaatcg aacagcggtt ttcaaacgat ccagagaatg gtattcatca
   129181 aaatgttccg cgacaatatt aatttttcga ctctgtaaat gaccgatcat gcaaagacgg
   129241 attgcatgtt ccgtttcttt aaatgcttct atcttcccga gagtttcatc cggataattt
   129301 tcgccgaaat atcgaatatt taaatcaatc aggcatcgaa tcacctcggc cgattttttc
   129361 tttgtcacag ccatcagaga aattttcgaa gattttctac cgcttttagc ggcagcctga
   129421 tcaatttttt gttgtatttc cgacaagttg tttctaattt gttctttgtt gatttctgtc
   129481 atcattccat cccggccatc gaaattaata cgccgaatcg ccccgttttt ccatgatctc
   129541 ctcgatgtga aaaccattca tccttatgac ataccgtaca aatatccatc ctttttatcg
   129601 tttttacacc cgcatccgat aaagttaatt catttgcttc ccacaagttg taacgaacct
   129661 cgtctcctgc ccgtatttga attttatcca agtcttttgg aaatgcttta ataaattggt
   129721 cggaaacatc ttgctgaacg acaaaatgat ccggtccgat tgacggacct attcccgcta
   129781 tgatattttg cggattgcat ccgtagactt tgaccatatt ttccaccaca ttttttccga
   129841 ttttcttaac ggttcctttc cagcccgcat gatagattcc ggctacacga aatatcggat
   129901 ccattaaaat aacggggaca caatcgccaa agcgcataac cagtgtgaca tccgggttat
   129961 cggtcaatag tgcatccccg cgaatagttc gggttcgaac atctcttgat gacttcacgc
   130021 agatcgttgt ggaactgtga acctgccatc cgtcataacg agtagataga tcaagattaa
   130081 aaacttgaaa cattcggttc aaattttcaa ggacattttc attgctgtcg ccgaccgttg
   130141 tagccatatt gagagaatga aaaggaaccg gactgactcc tcccaatcgg gtaaagaaac
   130201 cgtgcttaac tcccaatttt gcgaattctt caaactgata gtatcgtaat ccgtttgatt
   130261 ccttaaccaa ggtcatgctt catcctcttt taatttttta tccaaataag gaaataaaaa
   130321 ccacgctgta ttgaacccaa acatcgcgcc ggtgattaca cgcaaaatag gcgtactttc
   130381 tctggcgggg aaaacagcgg ggttgatcgc agcaaacaat tgtgaaaagc cgtctaatcc
   130441 gatcggaacc aatccgataa taatccacag gatccaatgc aatctgggaa tacgatagtt
   130501 tgaaatcaaa aaaataaagc agaaaagaac aatggcaaga taaatggcaa tatcccgttg
   130561 gcatagagca accttatagc ccatctgcgg attcccgaca aaagatttca aatcaaaaaa
   130621 atccattttc gcattttggg ttgcttcttc aaaagtgatg atatcgtgat ccatattcgc
   130681 cgatgcaagc ggataaaatg cctgctcccc gaacagaaaa aaagatcggt aaggaagttg
   130741 atgacaaaaa aaaccaaaaa acttatatat caactttccc ggaaggatca aatcataatg
   130801 cattaatacc ggtgccagaa acgccaaacc ggtataaaga gaaaaagcga aagcaaagat
   130861 caagatatag aagcgtttaa aaagcaagga taacttatct gttcccattg ttttttcgaa
   130921 aaatcaataa gaaagttcgg gagtttatat ccatcagttt ttgacctcaa ttaaagtccc
   130981 cacgtctccg gatacggtaa tatcgccggg atccaaaccg acttccggtt ttacccaacg
   131041 ccagatccat ttcgcatcgt ccggttttgc attgacacac ccgtgcgatc gcggagttcc
   131101 aaacgcgtta tgccaaaaaa caccatggat tgaagcgccg ccggtagcaa atatgacatt
   131161 ccaaggtact ccgggagtat cccaaccgga accggtcaaa ctgccggaca tatgcaacga
   131221 aatcgatttt ctccacggat gatgattccc ggtaggtgtg gcatatctat ccgtaagcac
   131281 cccttcattt gtatacatag ctcctgagga aatacggcag aaataaactt ccacatcgtt
   131341 ctcatagcac gttagtgtct gataagtcag gttcacataa attcgtttat tttctacttc
   131401 gggatgaatc ggtgccactt cttcatccgt gatttttcga attgcccgag catccgcata
   131461 gacaatgtcg ccataagttc cgtaaagctc attcgcccga taaagaatag tcccatccga
   131521 agcagttttg atttcgtcaa tccaaaatat ttgatcgtaa taaagcttgg gattgtgatc
   131581 ttttatagaa atataccatg gggaaaccgg atcttttccg aatatcaaat tgacataagg
   131641 gactgtaacc actccccaaa atcctttccc gatacttgaa tcgggtaaag cggtttccgg
   131701 aacatttggc tcatttttta ccaattgcac acccggagaa taaacatacc catagggagt
   131761 ttcaaaccaa gtggcattat atgttcctgc cggtgcttct cccacaactt cccggtatat
   131821 cgggaaaagg aaatcttcat atacgctttt attgatttcg gcatccgctt ccggcttgac
   131881 tcgaaaattc ataatcccgc tcaaacaatt tcgtccgata aattcggact gaggaaaagt
   131941 atccaattga gctgcaaaat caaaatacga gtgaacgttt ttcggaatca ataaagctcc
   132001 gcccgaaatt gtcgcaatct ttaaaaaatc tcgacgattt attttattct ctcgcataac
   132061 tttactcatc acgcctcgct cgctctcgat ctgcatctcg ttttgcaatc gcttctcgtt
   132121 tatcgtatag ttttttccct ttggcgattc ctatttccac ttttgccaac ccgtgcgaaa
   132181 gatacacttt tgtcggaata atcgtcatcc ctttcaaccg gatgctgttc caaatatttc
   132241 ggatttcacg tttattaagt aacaattttc tcggtctcag cggatcgtga ttgtaacgat
   132301 tagcggggtc gtaaggtgcg atatgagcat tcattaacca agcttctttc ccgtcattga
   132361 tttgaacata cgcatcggct aagctgattt gacctgcacg aaccgatttt atttcggtgc
   132421 cttttaaaac aataccggct tcgaaagttt ccaatataaa atattcgaat tttgcttttc
   132481 gatttgtcgc aacgattttt tcacccatgg gttttatttt agcataaagg attttcacaa
   132541 tcatagaaat accctcggat gtgtaaattt cgcaatcaca agatcaacac tccctcaaat
   132601 ggatagttct ttcatttgat aagtaagttt tggacccgga aaataattta taatatttca
   132661 aaatgatttg acttcttgat gaaggatagg ataggttccg tgcgacaatt aaaatactct
   132721 tttgataccg tttttcgaac ttctttatta tttctcattt ttttgttcct caccggttgc
   132781 aacactccga attcctcgtc attacagaac agttcctcat cgcaaacgcc ccaaccggat
   132841 cagacccaaa tttttgcaaa tgtcttagcg accgcaaatg cttctattca acaaacaatc
   132901 ttgcctgaga atacggagat aacgacgcaa gttgctgagc tgtccgaaac gaatattcaa
   132961 gataccggat tgccggctcc gacgtcggat cccaaccgaa cccctcccgc attaccggcg
   133021 aactttacaa ccgatttatt aaataaactg gatacacccc atacatatat agcggacacc
   133081 tgcgagtttt taaagaaccg gtggtcggca gggaaggccg aacccggaac cgtcgtaatg
   133141 gtcgtaatgt atcactcaat cgttcaggga gacgaatctt cagttactct ggataatcag
   133201 atttcgtctg aaaaaaataa acaaatcgcg gccgatctgt ataatcaggg atttaccgct
   133261 attgatacga ctcaatttat caactttatg aaatacaacg attacatccc gcctcgttcc
   133321 gttctattaa ttgtggatga tcgacatcat ggctcatatt ttgccgatca tttccttcct
   133381 ttttaccagc aatacggttg gaaagtcatt aatggatgga tcagttccga cgataccacg
   133441 caagagctgt gggacgaaaa tgcacaagtg gaagcggaag ggtatgtgga tcaccaagca
   133501 cacggcgttg tgcataacga aaacatgacg gatgcatcat cggatgattt tatttacaat
   133561 gaactgaaag gttccatcga tcgaattcaa caacatttcg gtaaaattcc gaaagccatt
   133621 atctggcccg gtggaaattt tggttttcgg cctattgaaa tcgcaaagga actcggatat
   133681 gaagtcggtt ttacagtcag tccacggggt cctgttatgt acaattgggt cccgctgtgt
   133741 gatgaaacag accccgctcg accttcctac ctggcggatg gattacaaga tcctcttttt
   133801 gtgattccca gatattggga tacggatgtc agtcagcata ttgataccgt tcgtcaaatc
   133861 ggaaaggccg cgaaagaaca aatcctggcg aatcgagaaa cggaattaga atattatgat
   133921 attgtatgca aaccgaaatt aggagaatta ggagtgcaac catgagaaat tttgattcgg
   133981 attgtatttt ttgcaaaatc gcaaaaggag aaatttcctc ggaaattgtt tatcaggatg
   134041 aatttgtcac agccttcaaa gatctctcgc cggtggcacc tgtccatatt ttgatggttc
   134101 cgaacggtca ttatttggac ttgaatgact gtgcggaatc cgataaagag ttgctgggtt
   134161 atattttgct tgtcggggca aaaattgcta agcaattggg gatcgatcaa agtggttatc
   134221 gcatcatagt caatgacgga ccggatgccg gtcaaactgt ttttcattta cattttcatt
   134281 tgttaggcgg tcgtcctttg gcatttaaaa atcaataatc ttgtgatttg atcaataaat
   134341 gaagattttt tacggtacat ctttactctt ttttttattc ttcttcttat taattcttcc
   134401 ttcgggatgt atgccggaag cacccaccag tgaaatcact ccgctggatt tgaacggtcc
   134461 catcgccagt ttatccgcag tcgtcgctat gaacctctcg gaaacacccg aacccactct
   134521 gattccgact tcaaccccca aaccaaaaat gaattcttct ctggcgcttc ccactcatcc
   134581 ggtatttcaa ttggatgcca ttccgacctt acgtcttgaa tcggttgaat acgaaattat
   134641 ggaaggagat acgatttcca aaatcgccac gactttccaa gtctctaaaa aatcgcttat
   134701 cgaagccaac aatctgatca atccggatat tatttccgtc ggacaaaaaa tcacaattcc
   134761 tccgcaaacg caagatattc gagataaagg gtttcttatt ttaccggatg ccgaattagt
   134821 atacggacct ccttcggttt cttttcaacc agaagatttc ctttctgatc atccgaaaag
   134881 ttacctgagt acctaccttg aaccgacacc cactcctttg ccgaaaacac aaaaagattc
   134941 ttcaaaagaa tcaccgatcc gatttacgcc caggacgggc atggaaattt tatttcaggt
   135001 atccgcggaa aattcgatca atccgaaagt gttgcttgca ttaatggaat ttcaatcggc
   135061 ttcgatttcc aattccaaac ccaacgatca tcagaaaaaa agcgtcattg catatctcgg
   135121 gaaaagctac gagagccttt caagacaatt aaattgggca gctgatacat taaattacgg
   135181 gtactatcaa tggaaaaata aacggatcaa tcaatggatc ttggatgacg acaccgttgt
   135241 tgccgtaaac aacaatataa atgccggaac cgcagccatt cagtatctgt tcagtcaaat
   135301 atacggaaag gatgattggg tctatgcagt ttcggagttc ggttttgcga tgacttatcg
   135361 aaatctattc ggcatcgatt cgcaaaatta ctcatgggag aatactccgg aatcatttcc
   135421 ggatctgcaa ttaccttttt cagccggaga aatatggtct ttcaccagcg gtccccatta
   135481 tggctggtct tccggtactc cttggtcagc acttgatttc gctccgccgg atgcgatcgc
   135541 atgcagtcaa agtaatcttt gggtaaccgc ttcggcaccc ggaaaaattg tttattcaaa
   135601 aggcggtttg gtcatcgaag atttagacga agattcaaat atgcaaaccg gttggtcgat
   135661 cctgtatctg catattgagg aacgagatcg aatcgaagcc ggccgaacag tagaatcggg
   135721 ggacaaaatc ggtcatgctt cctgcgaagg aggtatagcc gacggatctc atgttcatat
   135781 tgcgagaagg tataatgggg aatgggtccc gattgatgca aaacatcctt tgatcctaag
   135841 cggttgggaa gctttttcaa ccggttcaat atatgacggt tatctggtaa aagatgacaa
   135901 aattgttgaa gcctggtatt acaaaaccga agagagcgaa atatcctact agtcttttaa
   135961 taatgatcat aatgatcatt ttagttgggt tcccttcgtt cgcctgtaca cgttcggtca
   136021 gtaaacccgc tgaattgaca gaagcggcaa ttcgttttga aatcacaaaa ataggtatgg
   136081 agacttctgt tccgaaaaat ctacccttgg cggaagttca aaaaacctac caaccgattc
   136141 cttatcggag ttcaaccccc gttccgacaa tgccgtctta tccgataccg accgagacag
   136201 ccattaaagc cgacaacaat gcgaaacagg tagcgacaga aaaaagtcat ataacaccct
   136261 tccgctcgat atctactttg atgcccactc cgaggaatac gataccgatc gaaccgataa
   136321 atgccgatgt cacttttact cccgtcccat ccgtaaaagc ctatgtcccc gtcgaaattc
   136381 aaccgttaat tccgactttg gaaatcacaa taattcctac cgaagttgta ttgccggatc
   136441 tcgagtcgat caatacggag aaactgttat atgtcactca aaatggagat acgctgcaaa
   136501 atatcgccaa tcgttttggg gtaacgtcgg accagatttc gagtactcaa ccgatttcgg
   136561 caaatggttt tttaaatccg aacatcaacc tctttattaa cgctccgaaa gaccgtgtct
   136621 atgaatccgc agtgaaaatt ctgccggatg aatatatcgt cttttcgaag acagctaccg
   136681 attatgatct ccaaaatgag ataaaccaag cgggaggtta tttgtcttat tattccgaag
   136741 agacttcaga tggattaatg agcggaagtg aaattatata taaagtttgc agagattatt
   136801 caatcaatcc gattgtcata attgctttgc ttgaatataa gagtcattgg gtatacggat
   136861 ttccgcgcac gcccgccgaa aaagattatc cgatgggatg gattgaatcg acaaacaaag
   136921 ggctttataa acaggtctcg tgggcggcaa ccgcgctttc ggagggatat tacggttggc
   136981 gatacggaaa aatcagccaa attccttttt ataaatatcc caaacctccc tatccgattt
   137041 attttgaacc cacattaaat gccggttccg ttgcgattca atacttattt tctcagcttt
   137101 atgtttggga tacggtcgat gcagccatct atagcgagga gggctttttc tcgactttta
   137161 cttcgatgtt tggaaacgta tgggagaatt attcgcctga tccaaatgga ttgaatgcca
   137221 atttacgtca aatcgaatta acattaccat tttctaaaac agaaaagtgg gcaatgaccg
   137281 gaggtccgca tgccgcttgg actaccggtt caccttatgg agcattggat tttgcacctc
   137341 cttctcaata ccatggttgt atctcttccg atcgatgggt acttgccgct gctcccggaa
   137401 tcgtcgttcg caccggcaaa ggaattgtaa ttgtcgatat ggatggggac ggattagagg
   137461 aaaccggatg gtctctgatg tatttgcata ttgcgacaaa tgaaaaagta ccggttggta
   137521 cggtgcttga acgaggatca cgaatcggtc atccttcctg tgaaggcgga tcagccaccg
   137581 gaacccacgt ccatttagca aggaaataca acggagaatg gattaatgct acgggacctt
   137641 tgccttttgt tctgtcgaac tggacagctt acgaaggatc cgaagaatat ctcggcggaa
   137701 tgactcgcgg aaacgaaatt gttcaagcga gtacggtagg atcaagcgaa tcgattattc
   137761 gatattgata ttttctgaaa aaacgaatct ctttccctta tatgttaaaa tgcggtacat
   137821 gaaagtttta ttttttagaa acaaagtgat ttctttttca ctattgtttg ttgcagctgt
   137881 cgttttctta accgcttgcg gcacaaataa gagtgctacc gaacaaagtc agacaacttt
   137941 gacatttcct ttgcaactca gtccgacacc gaccgaatta tcatcggtat cggaaacatt
   138001 gtcaggggct tccaaatatt atgaaaaacg accaaattat atgccaggag agccggttga
   138061 ttatatcgtt caaccggggg atacggtttc ggcattggcc tctcgtttta atacaactca
   138121 atcggaaatt ttgaaagcaa atccgattat accggctgtc acgaccactt taccacccgg
   138181 tatgccgatg aaaattccta tctattaccg gagtcaatgg ggaagtccgt tccgaattct
   138241 tccggattcc ctttttgcaa atggtccggc tcaaatcggt ttttctgcca gaagtttcgt
   138301 tgaccggcag cccggatggt ttaaatattt tacggcttat accggtgaac aaaaccgacg
   138361 cggcggagag attgttgatt atatagcgac gacttacagt atcagtccgc gtttattatt
   138421 agcgctgttg gaataccaga ccggcgctct cactcaaaag catcgaccgg aaggcatcaa
   138481 tactaacagc gtttttggtt tcaccgatgt aaattcaaaa cagttaaccg ctcaactgaa
   138541 caaactttca aattatttaa ataattatta ctatgaatat cgagaaggca gagagcttga
   138601 gtatcaatta ttggacggat ctttatatcg cattgatcca tggcaaaatg caacaacggc
   138661 agctctccag cattattttg ccatgattga tcctccgaat gaatttttct atgcaatcgg
   138721 tccgaatgga tttcaagcca catatatcgc tcttttcgga gaaatagagg atcatccgga
   138781 ttatattccc ggtagtttga ctcaaccgaa gtttacactg ccattcaaag agggtgacag
   138841 ctgggctttt accggtggtc ctcatcccgg atggggtgac ggagctccca attccgctat
   138901 cgattttgcc cctccttctt ccgcaagcgg atgcgttccg agcgatgaat gggtccgcgt
   138961 tccggcgggt ggcactatcg tacgaaccga tacgggtgta gcggtgcttg atttggatga
   139021 cgataaagat gaacggaccg gctggaatat tttcttcctt cacttgctca cttcttccat
   139081 cccaccggtg ggaacgaggc tcaatacggg agatgcgatc ggtcatccgt cttgtgacgg
   139141 cggaacttca accggaaccc attttcatat tgcacggaaa tttaatggtg agtggatttc
   139201 agcgggagga attattccgt ttgagtttga tgattggaaa gtagaaaacg ggaccagtgc
   139261 ttattcgggg aaattaaaac gatattccag cacggttatt gcctgcgaat gtgcggatta
   139321 taccagcatc attcataacg gtccgccatt ggttccgact ccgacctcta ctgtcaagcc
   139381 aaaataagac aaaaattgat ttacagactc actcgtgaca atggccacgt cggaaaaaca
   139441 tccatcgcta aatcatcgaa tttatttgca ataaaccgct gatagcctgc ggctccgacc
   139501 atggcggcgt tatcggtaca aagagataat ttgggaataa ataatggaaa atcggattga
   139561 ttttcaaaca actcccgcag cgctttattt gccgacacac ccccggctat aatgatacag
   139621 cgagcgttat ataacctggc ggcgtccatt gttttttcat ataagattcc ggtaaccgcg
   139681 gattggaaac cggcagccag atccgaaacc ggcagctctt ccataccctc agtttgcagt
   139741 tctctgactt ttctgagtac cgctgtctta actccgctga aagagaaatc ccatgttcct
   139801 tcgagttttg atttcggaaa acgaaatcga ttcggatcac cgaaaaccgc cgctttttgt
   139861 atggaaggtc ctcccggata ttccaatccg atcaggcggg ctactttatc gaaagcctct
   139921 cctgcagcat catccaaggt accgcccagc cgttcataat gcaaatgaga ttgaattaat
   139981 ataatttccg aatgtccgcc ggagacgaga agagcaacag ccggaaaatc gggctgtgtc
   140041 agcgggggtt tatcttccga accatataac caggccgaat agatatggcc ttccaaatga
   140101 ttcacaccga tcaggggtaa tccggtcgct aaagaaagtc cttttgcggc attcataccg
   140161 acaaccaacg atccggccaa accgggtccg cgggtcacag caatcgcatc gagatcatca
   140221 taagacatgt gcgccttttg catcgcctct tcaatgacag gagatatcga aagaacatgt
   140281 tggcgagaag ccacttcagg aaaaactcca ccatattttt tatggatatc gatctgagac
   140341 gcaacaatat ttgaaataat tcggcagccg tcttcaacca ccgccgcaga ggtttcatcg
   140401 caagaagttt cgattcccaa aattctgatt tgttcgttat ttgtcatggc cttcctctgc
   140461 atttttatca ctatatggtg caacatccaa gacaaattca taaggatacg gagcaataat
   140521 gtttgctttt ccgttgtgat cgataaatac agtgtcctca atccgaatcc caaacggttc
   140581 cttcgcttcg ggataataga gccccggttc taccgtaaaa acggtaccga tctccgcacg
   140641 gtcggaatcg ggagcggaaa atccgctgaa cggtctttca tgaatattta atcccaagcc
   140701 gtgactgatg gaatgcacgt agccttccaa agtatgagga ctattcaata cggttttatg
   140761 acccattttt tcaaacattt cacaagtttg tttttgtaat tctttgagtg gtttgccgac
   140821 ttctatggaa ttaataatcg catcgtgaac aacttttacc tgtttataag cattaagcaa
   140881 attatcggag gctttgccga tacaccatgt tctggtaaag tcataaaaat atcctccacc
   140941 catttcgcac ggaaagaagt caaaaacaat ggtcttccct gtttcaattg tttggttgtc
   141001 atctcccgaa ttatgcggaa cagccgaatc ttttcccacc gaaaaaattg ttccttccgg
   141061 attttctccg ccgagttcgc tcaaccacag attaattttt cgtttaatat ccccgattgt
   141121 cacttcctcg ccgtctttgc cgacaagcag atttcctttg atccggcaat catccttaat
   141181 aaaattttcc acacgtctga caacttcggt taccgtcaaa cccatcttac ggattgcttc
   141241 aatttcctca agatcttttg tgaatcgcgc ctgattaata acatcgattc cttcggaacc
   141301 gagaatttca aattccggaa tcaactctcg gaatgcttgt accatagcat attgtgtccc
   141361 gaattccgtt tttccgcaga aggcaatttt cccgttgcga agtccaaccc gattaagaat
   141421 atcgcgcaat tccaatgcat ccgcttttgc ctgatcgcca tcccactctc gtaaatatgt
   141481 ttcagtagga aattcatcaa aacaaatctt cttcatgccg gttttcgcgg cattatctct
   141541 ttccatcggg cgataaaata agaccggttc ccggtctttt aataacacaa taatagcttt
   141601 ggtaaaaaat tcgccttttg ctaaataagt catcggtggg ttatgttcac tgccgcctga
   141661 aacgataatt gcatcaacat ctttttgttt cattaaattt tgaatatctt ttttcatttt
   141721 ttattcccga acgaagattt ctttattcat cttttttcat atcatcaaag cctgatcgat
   141781 attgaaccgt atcccgagtc cgcagaagaa tttcaattaa gataaatcct ccgacacaca
   141841 aaaaaataat tgtcgaattc agcactcgtc cgatttggaa ccacaataaa atatcacaaa
   141901 gcaatccggt gaccaaggat tcccgaacca cagtcttagt gatgattttc cggattgtga
   141961 aaaaatattt attcagggct gcatagatgg gcatcataat tccggtaata aaaataatca
   142021 tcagaaaaaa gaacaaccat ctctccttca ggtaaggaag agtattcatt accaaccaat
   142081 aaagaccata tccacttggt aaagaaaaaa acagcccgag aaaaatatat gttcgaagtt
   142141 tgtccaaacg gactccttat ttgcctatcc gatcgaatac tattataatc gcattgagtt
   142201 tcgtcaaaga ccagccgtat atttcgaaaa cacatataaa ataaaagaat cgaacatatt
   142261 tataatattt cttaaaaaac gataaatgat ggagaaaaat gctttttaca gttgacatcg
   142321 ggaatacgaa tatcacattg ggtatgtttc gtggggaaga tttaatcatg cgttggcgta
   142381 tggcgacaaa tcggaattcc atgccggatg aatttgggat gcaattttgg tttctttttg
   142441 aacataatgg cattaatccg gagaacatcg aaggaatttg tatttcctcg gttgttccgc
   142501 agataacttc gaaggtggaa gatgcttgtg attattattt taaacgtccg attctcgagg
   142561 tgaattacaa attaaaattg ggtatgaaaa tcgacattga tcatccagct gagctgggga
   142621 atgatcgaat cgcggattca gtcgccgtaa aagcccttta cggaactccg tcttgcctga
   142681 ttgatttcgg aacggctacc acatttaata ttctcaatcg ggaagggaat tatgtaggcg
   142741 gtgctatttc agccggtata caaaccagcg ccgatgctct gtttcttaaa accgcacagc
   142801 ttccgaatgt taatattcaa aagcctccct gcgtaatcgg caaaaatacg gtaaacagca
   142861 tgcaatccgg tcttttttac ggttatattt cgatggttga gggaatggtt gaacgatgca
   142921 agcaagagtt gggagatgag gatatgcaag tgattgcgac ggggggatat gcaaagattc
   142981 tttctcagga aacaagttgt atcgataaag tggattcctg gcttacgatg accggcttac
   143041 gtttaatttg ggagatgaac caatgaatcc tttaaaagat aaaaaaatat tgttagggat
   143101 aacgggatcg atcgccgcat ataaagctgc ggaaattgct tcttctctga cgaaatccgg
   143161 tgccattgtc aatgctgttc tgacgccggg cggtgaaaaa tttatcactc cgctgacact
   143221 ccattcggtc accgggagac atgcctcggt tgataaagat ttatgggaag gagaacagca
   143281 cgttacccat atcgaactgg gacataattc cgacttgatt gtaatcgcac ccgcttcggc
   143341 ggattttatc gcaaaaataa ctcacggatt tggggattcg ttgctcgcac tggctgtttt
   143401 agccggacat tgcccggtat tggttgctcc cgcgatggat ggagatatgt atctgaatca
   143461 agcgacgcaa gagaatatcg ctcagctgat gaagcgtgat tttcaaatca tcgggcccgc
   143521 ttccggtcat ctggcttccg gattagtagg cgtcggtcgc atgtcggatc cgcaagaaat
   143581 tttgggcgcg atacgttaca cactatctcg cggagggatt ttaaaaaata aaaagatatt
   143641 ggttaccgcc ggaggaacac aggaggcgat tgatccggtt cgtatcattt cgaatcgatc
   143701 ttccggaaaa cagggatatg cgatcgccca ggcggcattg gatttgggag cagacgtaac
   143761 acttattacg acggtcacaa cgcttccaat cccctatggt tgcaaagtcg tttatgtaaa
   143821 atcggcaaag gaaatgcaaa atgcgatttt tgagaatcta acggatataa atgctctgat
   143881 tatgtgtgcg gcagtagctg attttcgccc cgaagtatcc gaaaaagaaa aaattaagaa
   143941 agacggatca gagttgttac ttcgattgga gcctacggaa gatattctgc tggaagttgc
   144001 gaaaaggcgt tatgaataca cgaaattaaa tgccgttatc ggatttgccg ccgaaagcga
   144061 aaacctgatt aaaaatgccg aaaataaatt aaacaagaag aaactggata tgatcgttgc
   144121 caatgacatt tccgggaaag aaagcggatt tgaaagcgat aaaaatcaag cctgtttctt
   144181 gttcccggac ggatcaaaaa gagatttgcc cttaatgggt aaagaccaac tcgccgagct
   144241 tatcatggag gaaataacca aaatcataat aaaacaatga caagaaaggt tatatccttt
   144301 ggaactttga ctccattaat ttcgttttcg ggtaaaagaa gttatagaaa tattatggag
   144361 taaaaatgaa aagaaaatcc atcttgttaa gcttgatagt aatcattctg atctgcggat
   144421 gcagcaacag aaaatctccg gattcctctt cccaaagtga tcgctccatt tctcaatcaa
   144481 ataatcccta cttagctgat tatcaaggag aatcttcgaa ttacaatgaa gccgcatatg
   144541 cgcctaaaat ggcttctctt gaaagtcaat caaattcgga agtgactcga ctgatcgtaa
   144601 aaacagcgga tatgacgatt gaagtccaag ataccaatca ggcgttggaa aaaatcagca
   144661 acatcgccac acaatttggc ggatttgtcg tgcaatcgaa tacttggaaa gaatcagaat
   144721 atgaagaaca atcccttctt tatggcgaaa ttacaatccg cgtaaaagct gaaaacttcg
   144781 acgaaactat tcgaatgatt gaaaatttga caccaaattc cgaaaaaaat gtgttgagca
   144841 aggttatttc gggaaatgat attaccagcg attatattga tacaaaatca aggctgacca
   144901 gtcttgaaaa caccagaaca aaattaaatg agatcatggc aactgccacc accgccgaag
   144961 aaacattagc ggtttataaa gaaatagcag atatagaatc tcaaattgaa gttctgaaag
   145021 gacaaattaa atacatggaa gagagtgctg ctctttcttc cgtcactgta aaaattcatc
   145081 cgattcaacc cgaaaaaaca atcgaagtca aagaatggtc aatttccgaa attttaaagg
   145141 gcgctgtcca agatctaatt gattcggcac aagattttgg agaatggcta atttactttg
   145201 ttatttctgt cctaccattt ttaatcatca tatttgttcc gatctatttt atcgttcgag
   145261 cttgctcgag aaggagagag aggaataaac agaaaaaagc agacagcaaa aaaacgaccg
   145321 atcaaaaaga taatcaaccg taagatcaat acaaatccgc tcattttttg aagatcccga
   145381 aggaaaattt tgggatcttt tgtttgttga tcccggtgat aaaatcattg gtaaaatggc
   145441 tgcacgaatt tccgaaaaag aaaaagaaca gttaaggcat gcgggtgaaa ttgtttatga
   145501 aaacatcaca cccgaaaaag acccggtata tacgccgata ccttccttcg tttcggataa
   145561 agtaatctcc ttgctttcgg cgagaggcat tcgattttta tatcaacatc aattgttggc
   145621 attagaagct gaatttaaca agaataatgt aatcctttcg acgggaaccg ccagcggaaa
   145681 aagtctttgt tatcaaattc cgatcatgga atatctatta agggatcccg attctacgat
   145741 gttattaatc tttccgacga aagccttgac gcaggatcaa cggaagaact tggcatcttt
   145801 gattccggaa aaaatacagg aaattgccat ttatgacggt gatacgccgc cataccagag
   145861 agccgcaatt cgtagaaatg cgcgtgttat tctcaccaat ccggacatgt tacataacgg
   145921 aatgcttcct tttcatcctt cgtgggcacg tttttttgaa aatctgaagc tggttgtttt
   145981 ggatgaagca catatctatc gcggggtttt cggcgctcac acggcgaatg tcattcgaag
   146041 gttaatcaga gttcacaaac attatactca gtcttcttta tctcctcagt ttatcctctg
   146101 ttcggcaact ttatcaaacg ccaaacagtt ggcagagaaa ttggtcggct ccgaagtgac
   146161 agaattctct caagatactt ccggaaacgg agttcgggaa atattttttt taaatcctcc
   146221 gatcgtcgat gaagcgcttc atcttcgagc cggttctatc tataccgcgg caaaagtcgc
   146281 ggaaataatg gaacagaaaa atcgccaagt cttattgttc tgtcaatccc gccaatcggt
   146341 cgaatctact gtacgaagat taagggatat cggtatcaat gccgatggat atcgttcagg
   146401 ctatttatcg aaagaacggc gaaaaatcga gaacggattg aaagaaggct caaatcgttg
   146461 tgtggtcgcc accaatgctt tggagctggg gatggatatc ggcggtatgg acagtattct
   146521 ctcgattggg tatcccggat cgattgcttc atattatcag cggatgggaa gagccggcag
   146581 aaaccatcaa ccctccgatt tcatcatgat tgcatctcaa aatccgatcg accaatatct
   146641 gatgaagcat ccggactata ttaccgaaca aaaagcggaa ccggcattaa ttgatccgga
   146701 caatcttttg attttatacc aacatcttca atgtgcgtta tctgaagttc ctttttcttt
   146761 ttccgaatcc tacggcggtc tgacgattga cgaaactcag gaattgttag agagtttttg
   146821 ttcgatggga atcgcccgaa aaagttccgg acatttttat tggcttgaat catcttctcc
   146881 ccaacatacg atttctttga gaaacagcgg gttagatcga atttcgattg tctcacagga
   146941 tttcaatggg aaaaaagttc cggtcggtga tatcgatcgt ccttcagcat attggatggt
   147001 tcatgaagga gctatttatt tccataacgg agaaagttat cgcatcgatg agttgaactt
   147061 agatgaaaat acagcatttg cccgtccgat cccggctgag tatacgaccg aagcggataa
   147121 aaaaacaacc attttgacaa agaatgttct tatgaaaaac tctgtacaaa atgcggattt
   147181 atcggctgcg gaagtcacgg tcactacgca agtgattggt tataaaaaaa gagatatcga
   147241 gacaaatcag attttgggaa gttatccttt agatttaccg caagaaaaat tagagaccaa
   147301 ggcatttatt ctgaatttga gcgatcagtt tcgcgaagaa cttcgaatca acagccattg
   147361 gacaaatgat cccaatcaat atgggccgaa ttggtttaaa attcgcaaaa cgatcctcga
   147421 acgagaccat tttcaatgtc agctttgttc tgtttccgga accgaacaaa ccctccatgt
   147481 tcatcatatc attccgtttc ggaactttac gaatccggtt gatgccaatc aaccggaaaa
   147541 tctgacgacc ctttgtcata catgtcatga tcgagtcgaa gcggccgtga aaatgcgcag
   147601 cggattaacc ggctttgctt cggctttccg ccagatttca gctctcttcc tcgaatgcga
   147661 cgccaacgat ctcaatgtga tcagtgatcc tgcttgcgcc gaatatgatg ggaaagcgac
   147721 aatttatctt tacgagacaa tcccgggagg gataggcctt tcacaaaata tattttataa
   147781 ctttatcaaa attgtaagcg cggtgaaaga tttaatcgag acctgtgatt gtgaagacgg
   147841 ttgcccggga tgtgtcggtc ctgcaggaga aatcggcatc ggcggaaaag aagaagctgt
   147901 tgcaatcgca tccggtttgc ttttgctctg acctgtttgt tacaattaat gtatgggtga
   147961 tattctttct caattgaggc agttgggtgt aacacttgga atgccggaaa accaagattt
   148021 tgttaatggg aggcattcaa gcacacttga tgcgctaaaa attacctttc cgaacggtat
   148081 cgtcggtgaa aatgaatttg gctcatattt tatcaaccgt aaaatttatc cgatgccaat
   148141 gatgcacgga tgcgtcgaat tgaatgatcc gctcatttta accgacaatt tattatcaat
   148201 tttgggaatc gattcgattg ataaaaaaag agttttagca atggataccg aaacaagcgg
   148261 tttatcggta gcttcttccg catttgtttt catgatcgga atgggctatt tttttgaaga
   148321 cagttatatc attgaccagc tgattttacc cgatttaggt gaagaaaaag cctttttagc
   148381 tcaaattgag aatacctttg ctcgttttga cactattttg acttacaatg gcagatcttt
   148441 tgatattcct atgattagca gtcgcgaaaa cttccattac ttcccgaatt cttgtaaaga
   148501 tgtgaggcat attgactttt tgccgcttgt acgaaaatat tggggaaagt ctcttgaaaa
   148561 ttgccgactt tcgactttgg aacgagatgt actgcaagtc actcgcggga tagaagaagt
   148621 tccgggatat ttagctcccg agtattatcg ggaattcctt tcttcgggtg acgccgaaaa
   148681 aataaaagga gtcgcatacc ataatcagat cgatgtcttg agtttaagta cctttctgat
   148741 ttatctgagc aacctgatgc agttgaattt aaatgacact gagatcaacg atcaatgcgg
   148801 aatctcaatt caagaactga gaaaaaatct attgaatcat aatccgattg acaacccgga
   148861 aatcttttgc gggaatactt ttcctgtcgc agaccgaaag aaagcagcgg catatctgca
   148921 aaagcacaga gaaacagaaa aagcgatcga aatatatcga tctttagtaa cggaaggaga
   148981 tgttgacagc tgccttgctt tatcatccat ttattccaaa tcgacggaaa ctaccccgca
   149041 agcaattgaa tatttgaaaa aaggggcgga atttgtcgaa tcagatcttt gcatgggtat
   149101 ttggtcaaaa aaagaaaaac aagaaaaaat taaaactcag atcgaaaaaa tggaaagaaa
   149161 gttgagaaag aatcatggcc gggaaaaatg aaatcatcta ttgggctgaa aatgaagccg
   149221 aagcggtagt tatcgttgcg attttggaag aaaacggaat cccggcaata aaattgcggg
   149281 aaagttacgg cgctctaaat aatctttctt tcggaatttt tggagaaatt gccatcgctg
   149341 tggacgaaga tcgagcggaa gaggcaacag caatcattaa tagacagcaa gaatccgcag
   149401 caaattcaga cacgaatcag gagaatgatg aaaatgatgc atcatccgat gagattgata
   149461 aggacgatcg cgatgatcca ttttaaactg gataaagccg agaagaggtt tctcggtttc
   149521 tttatgcagg accttataat ccgattgttc ggatatcttc caatgccgag ttgatgtctt
   149581 cggtaaaagc ttgcattaat tttttggtaa tcggacccgg agctttttca tcgatttttt
   149641 gattatcaat gctgcgtatc ggaaggatag atctgctggt acttgtgata aatgcctcat
   149701 ctaaatactt caattcatgg atagaaacag gtcgaagatt taccggaatt gcaagctttt
   149761 tcgctaaacg cagaacataa tcccgcgttg ttccgaataa tacaccttct tcggcggtaa
   149821 atatttcatc tcgaataacg gcgtaaaaat tactgctcag cccttccaaa atttcattat
   149881 tactgtctac cataagaatt tcttcatatt cgaatccttt ttgtctccga acctcatttg
   149941 cctgagaaag aaatttgttt agctttgctt ttggattcga tcgatgtaaa gttgtcgtaa
   150001 tcacatcgat tccgttttca taacatttta tatccggtgt aatcaaagtc tccaagacga
   150061 tatagatatc tcccaagcaa acggataggt caacggttat gcgaatcctt aattcattgt
   150121 tcggatattt ttcgatgact tctccaagga acttgcaaat ttctttccga ttcagtaaaa
   150181 ccggtttccc ggcaagtctt gccgtctctt ccagtctgtc aaagtgcttt atcaggttca
   150241 aaacaaaaaa ccggttgtaa gtccgaaatg ttgtataagc cccatccggc gtatatcttg
   150301 aggaatcatt tatagataca aattttccct cggaaagcga agtgattgag taatgatcgt
   150361 ttacagcatg taacttccaa aaattatcca ttttgtttgt cccttatttc cgtatatttt
   150421 ttacacggaa tttatttttt cgaataatcg atcagatctt cttttgtgtc aatatctcgg
   150481 acgacaaaag gatccttgac ctcgacatat tttgcaccgt attgcttgat aatattgcgt
   150541 cctcccagat cattcgccgg ctgatcgatt aatccttgaa aagtccaatg gggaaacatg
   150601 gtcgggtttc cgttttttcc ctgataggtg ggcacaatta tactttcggg gttctttttt
   150661 tgaacctctt ccaatttccg tagagttcgg attgaaacaa agggttgatc agccagcatt
   150721 aaacaagcgg cgtcgacctc attttgaatg agttcaattc cttttttgag agaagtcgat
   150781 tgtccggatt gccaatccgg attctcaatg caaagaacag gtagtccttg cagttgaacc
   150841 aaaatatcat ctttttgata tcccaataca acaatcaccg gtaaaaagcc ggcttggagg
   150901 gctttttctg ccgaataacg aacgacagcc tttccgttta atcgaaccaa taacttatgt
   150961 tgctcggctt gaaaacgact gccgttccct gccgccaata cgattgatcc gattttcata
   151021 aactttttcc ctgtttcatt atgccctttt gcgcttatta ttatatccaa tccgtcggca
   151081 gaaacatggt cgatccggaa ggtatcatga aatgggtaga aaaatcaatc tcttcaaaga
   151141 aattcgtccg aatcccggag aaaagatcct catttatacg gcgggtatat gctatatttt
   151201 atttatttca attgatttca tagagtttaa tccctttaat ctttcggcga aattaaaata
   151261 tttatcaatt tggatttgct tcggaattac actgtttcga tttttctcaa tcaatcgccc
   151321 cggttgttct atggaaaaga agatcggctg gattaatata acggcatttt tctttacact
   151381 cagcgccgat tactttcttc tttttaccga acaatacggt ttgggaatta cgcttttttg
   151441 cggagttcat atagcgcgct tatatctttt ttcgacgctt aagaagagaa ctgaaattat
   151501 tttttgggaa gcattctttg cttctctcat cttttttgtt tgtcgatcct tcataatcgc
   151561 tatagcttcg ttctatgggt ttttaatcct gacagtgaca ataatgtctt ttcggaagct
   151621 gacattttta gcggatcgct accccgtttc aaaatatttg cttcggtttg gcatgatttt
   151681 gttttgcctc tgtgacatct gtgttgccgt atacaatttt cccggttcaa gttattttat
   151741 ttcgcggtta agcggcttct taatatggtt tttctattta ccggctcagt tgttactctc
   151801 tttcctgata ccgccaccct tatcaaaata aataatccgc catattccat ctttggcgga
   151861 ttatttattt ctgactttta tttgatttta tttgacgccg tgataagcat taaatttttt
   151921 ccaaacttcg ggatatttct ttaaggatct ggcacaaatt tctttttcat ccattgtggt
   151981 taattcgcga tttttcatca aaatctttcc gttgacgatt gttgtcgtaa tcatcctttc
   152041 gttgaatccg aaaatgatgt gccaaggaat atttcccgca ttcaaatcgt caaatggtcg
   152101 atattcgacc aggattatat ccgcaggtgc accgggaaca atcttgccga atttttctcc
   152161 atatccgaag gattgtcccg caagtcggga attattgtaa atcgccattt cctgtacatc
   152221 ataaccgttc atccttcgcg gatcccgatt cattacttta tgcagaaaat atgcggcttt
   152281 ccactcactc cacatatcaa aagtaaagcc gtcatttccc agacagacat tcatcccatt
   152341 ggctaacatg gattcaactt gcgccgctcc taccgcatta ttcatatttg agcgcggttg
   152401 atgagagatc catgtacctg tttccttcaa catctccatt tcagcggcat caatatgcac
   152461 cgcatgtgca acaatcgtct tgtttcccaa aatgtgatga tcataaagcc gtttcccgca
   152521 gcgttgattg aaccgataca aactgtccca ttcgtcctcc tgggattcgg cgagatgaac
   152581 atgaaatcca aaaccttccg gaatcgcagc ggcgcaatcc gaaagagtgt cttcatctac
   152641 cgataaacag gcatgtatac cgaaagttcc cgaaagacgt ccgtcaaggg tataaccgga
   152701 tttgactctc ttcagaaatc gaacattttc tctaattccc gccttggttc cgtcttttcc
   152761 gtttcggtct gttacttcat aacatagaga agcccggata ccgctcttat caaccacgtc
   152821 agcgataaca tccaacgatc cgtcgatgaa ttccggtgat gcatggtgat caaatagcgt
   152881 tgtacaaccg gatttgattg cgtctatgat aaatacttcc gctgataaac gcgtgctttc
   152941 ttcatccaaa gatcgatcca gtttatacca aagattatcc aatatctccg gaaaatcttt
   153001 cgccggtttc ccgggaatcg ccaatcctct tgaatatgct ccgtaaaaat gggtatgagc
   153061 gcaaatacta ccgggcatta ataattgatc atcggcattt attttttcct catcaggata
   153121 ttttttcagc agtaaatcac ttgaaccgac ctcttgaatc actccgtctt tgatataaac
   153181 tccggaattt tcaagaatcc gattgggttt ttcccaagta actactttag cgttatagat
   153241 taacattatt ttctcctttt ttctcttgaa ttttggactt caggaaatta aaagactttt
   153301 tataacgaga agagctttat ttcgtataaa aagtctttca gtaaatcgga taaaatccat
   153361 cagctcctca cagtctcccg gcggcgcttt tcgatgggaa tcaccaacgc cgatgtaaat
   153421 aaaatacacc gacctccgtg ttttcattgt agcattttcc aagttgtctt acaatctttt
   153481 attggttatt tttatcacta tttctcatta catctttcac gtataacgag atatttataa
   153541 ttctttggtc aaataataat ggcataaaaa gatgttatga agtcgatgga aaatttttaa
   153601 agaaccgctc gatgaaccaa agcggcacga ttcaacttta tctatgatgt atacgaagat
   153661 gtatacgaac tatctttctc tgtgaaccaa atcaaattct ttcactcgat acacttagaa
   153721 ttatcacacc ccttcgcccg acattgcata aaatcaaaaa ggtagattat aataacggac
   153781 gtcgttcggg gcgtggctca gtccggatag agtgcacggt tcgggtccgt gaggtcggcg
   153841 gttcaaatcc gcccgccccg acacaaaaca cctctaaaca aggtgttttt ttattcaaat
   153901 ttccatttta atgaattgtc ttacaatagg caaaaaacct ttacggatca gatctttacg
   153961 ttacaattac ttgaaattcg aataatcatt catcggagcg gaaaaagaaa tgagcaaacc
   154021 aaccatcatc agcagtcccg aaattttact tcccaacaaa aatgtcgatc tgacaaaatg
   154081 ggcaaccatc gcttgcgacc aattcacgtc caataaagcc tattgggatg aagtggaatc
   154141 aatcgtgggg gattctccct caacgttgaa aatcacattt cccgaatatt atctcggaaa
   154201 aatcgatgag gcgaaaaaga ttcaagagat tcaggatacg atgtaccgat atttgagaga
   154261 taacttattt gttactcaca atgggatgat attggttgaa agacaaattc ataacggatt
   154321 acgttatggg ttacttacaa ccatcgattt ggaagagtac gattacaatc gcacttcgac
   154381 cagttggatt cgagcaacgg aaggaacggt tgcggagcgt attccgcctc gcgtgcgaat
   154441 tcgagaaggt gctcctctgg aggtgcctca cattctcgta ttaatcgatg atgaccaaaa
   154501 gcggctgata gaaccactgg ttgcccaaaa ggaatctctt ccggttctat acgattttga
   154561 cctgatgcta aacagcggac atgtgaaagg ctattcattg gcagaccccg aactggaaca
   154621 aaatattatg gatgtaattg ccaaatgcca atcggatgaa gaaattacca gaaaatatgg
   154681 tccggtgagt gaaaaaaaca ggattctctt cgcgatcggt gacgggaatc actccctggc
   154741 gaccgctaaa gcaatctggg aaaaacataa atcggaatgg ggaatgaatc atcccgcacg
   154801 ctatgccttg gttgaaattg aaaatctgca tgatccggta ctggaatttg aaccgattca
   154861 ccgtgctctt ttccatgaaa caaaatcatt aataagcctt ctcggagatt atttccaaga
   154921 ctcgatggat gtcactgaga tgaaatccat ggaacagtta tttgccgata tcgaccatac
   154981 tccggatggc gttcaagctt tcggagttct cgaatcggga aaatattata aagtagtact
   155041 aaaccgctgt tcttctgcgt tatgtgtcgg ctcagtccaa caattcttgg atcgatgtaa
   155101 gtcggatcac ggttttgaag aaatcgatta tatccatggg aaagaggatt taatacatat
   155161 ttcttctttg gaaggcaatg ccggtatcta tcttccgccc gtccaaaaaa acgggttttt
   155221 tcagagtatt attaatgacg gtgctttacc gcgtaagact ttttcgatgg gtgaagcaca
   155281 tgaaaagcgg ttttatattg aatgcagacg tattttatag gctggaattt gctcaaaaaa
   155341 taattgtatg aaactttcga tataaattaa gcgtcttaat ttggtacaat acttgaaata
   155401 caatgtaaag aattatggag gattccatga aaaatcgaaa aaaactggca ttggccggat
   155461 tattgatcat tgttttgtct atcttgatgg tttcctgtgg ttcaaggccg acaccggagc
   155521 ccactgttga tgtgaatgta ttagcgacca atgcagctca aaccattgaa gcgagattta
   155581 cagaaacagc tttagcaaaa ccgaccgata ctccgcagcc gacttatacc ccggtttctt
   155641 atactcctac aacgagttcc gcaccgatca cgttacctac ggccggaaca ggtggcagcg
   155701 tctctatccc gacaatcgca caacagccta ccgcaatcgt caatttgccg acggctactt
   155761 tatccgcggt cggagataaa gccgtttggg ctgatcaaag cataaaagac ggcacacact
   155821 tcgcaccggg agaaagtgcg gatcttacat ggtatatcac aaatgtcggt acgacaacct
   155881 ggactaccga ttattcggtt cgattcttta cgggaacaaa ctttgcaaaa gccgggaata
   155941 cccgttatta tcttcccaaa ccggtagcgc ctcaggaaac aggccaggtt accatcgata
   156001 ttgttgctcc gaccacaccg ggggaataca agatggcatg ggttctgtca aatgctgaag
   156061 accataactt ctatacagtc gatatcacga ttattgttga ttgacggaaa gatacgatat
   156121 gaaaacaaaa atcttattga tcgttaccgg gcttattttc atagttgccg gtttcagcgg
   156181 catttcatcg caacaagctc cttcctctga tgcgaatgcc ttgcgaactg aagtcgccgg
   156241 aacggtgatc gcagaaatct attctaccgt tcaggcggaa ttttcgacaa tcgcgactca
   156301 aatggctaaa acacctgaag cacaatcggg cattatcggc gagggttttg tcaaaccgac
   156361 agaagtttta tatgcccata tggcgaagct tacgaagcag gataaaaact atcttcaata
   156421 taaaatgtac gaagattggg atgtaacttt taccttcaag aatgtcggct tgcaggattg
   156481 gacggatgaa ttttatttac gttactacaa aggtgaagta cctatcgaag gaaagataat
   156541 atacctgcct gctctgccga gaggagaatc aacaagcgtc acgctccatt ttgacggaaa
   156601 agatgaaccc ggaatttaca attcatattg ggaattaatc aacaatgacg gagccgtgat
   156661 cttagataat atttttgtgg cgatcttagt aaaataaggt tattgaaccg cttttttatg
   156721 gattcttccg aaatcaagga aataataaaa gccgaagctg agaagctcgg cttttgtttt
   156781 tccggtgtga catcaccgga catgcccgat gattttttca gatataggct ctggttagaa
   156841 aatcgttctt atggacaaat ggagtatctc aatcggaaag atgccgttga aaaaagacaa
   156901 taccctcagc ttttgcttaa ttcggtgcaa tcaattgttg ttttgggcac accgattcag
   156961 ccttcacctt taaccggatc tttcagaatc gcggcattcg cacattataa agattaccat
   157021 cgtcaaattt attcattaat cgaagagctg attcgacgga taagcgaaat tgcgggagaa
   157081 aaaccggaat acaaaatatg cgtagattca tcccctgttt tggaacgctc attggctgtt
   157141 caagccggtt tgggttggat cggaaaaagt tcaatgttta tttcgccgga ggcgggatct
   157201 ttggttctgt tatcagaatg ccttttatcc tggaaactgc cttatgataa tccatatccg
   157261 aatgaccggt gcggtaaatg cacacgatgc gtagatgcct gccccacaaa atgtatcgac
   157321 cctcaattac gaactataca agcggatcat tgtatatcct atctaaccgt tgaacacagg
   157381 ggggatattc ctgcggagct tgcaaaaaaa tgcggtaatc aaattttcgg ttgcgatata
   157441 tgcaccactg tttgcccttg gaatcaaaaa acagataccg gttattctcc attagaacga
   157501 tttcatttct cagataatct ttcggatgat ttgaatctta ccgaagagga atttaaaaag
   157561 aaatataaag atactgccat ctatcgtgtg aaaaatgaag gatggaaacg aaatatcaaa
   157621 atagcacttc aaaatctcta tgaggattaa aaggaaggaa ttcctgacaa aatagctccg
   157681 ccatgtttga cggagctatt ttctcattgt ctctaaaatt tatggtacag gtgtatatgt
   157741 cggaacggaa gtgggtgtcg gtgttacgat attgactgca atgataattg tggagcctgc
   157801 ggttagcgga gcattttcat cttcgatctt atttaactta ataatcgatt caattgtgga
   157861 gttaaatttt gatgcaatgt ctctcagaga atcattcagt tgaactttat attcaatttg
   157921 ttggcctgct gtatattgat caagcgcaat cggtgtttcc gtaggtaatt tcgtattggg
   157981 accgggaata ataacttcct gtccgggtgt taaattacaa ttatccatcg taaacccgtt
   158041 aattgccaga aaaacatcaa ttccgacttt aaatttattt acggccacat cccaacaatt
   158101 atcaccctct tcgactgtat acatttgggg tccttcaggt gtcggagaaa ttgtcggcga
   158161 cggagtaact gtttcggtag gtgttatagt cggcgtggaa gtcggaacag tcggtgaagg
   158221 tgtaaaggtt tctgtggaag tgggcgtcgc cgaagcgaac aaacctccga gtgagaaatt
   158281 cccgtctcct ttaatccata acactaataa taaaatcccc aagcctaaga taatcgcagc
   158341 aattgcccat attacatatg gcagcaccct tcgctttttt tgatatgaat gaatcacata
   158401 ttgagggcta ttttttcgtg ccattgtgca tttcctttca ccgttatcga tcaacggatt
   158461 tccttctctt gagttatttt ttattttaca cggtattcac attctttgca aaatatatag
   158521 aaattatccc gggttctcct gttccaaaac ttttttcaaa aattgtcccg tatatgattt
   158581 tttattttct gcgacttcct ccggtgtacc ggtcgtaaca acctttccgc ctctgtcgcc
   158641 gccttcgggt cccagatcaa tgatataatc cgcgacttta atgatatcca tattatgttc
   158701 gataataatg acggaatttc cttgttcaac cagccgttcc aaaacatcaa tcaaacgatg
   158761 gacatcggca gcatgaagcc ccacagaggg ttcatccaaa acatataatg tttttccggt
   158821 cattcgcttg gataattctt tggaaagttt gaccctttgt gcttcaccgc ctgaaagtgt
   158881 atttcccggt tgcccgattc ggatataccc cagaccgaca tcctgtaaca gggataattt
   158941 gctttgaata atcgggaaag ccgaaaaaaa ttcgatgccg tcatcaaccg tcatatccaa
   159001 aatatccgcg atggatttgt ctttaaattt cacctgtaac gtttcattat taaaccgttt
   159061 cccgtgacaa acatcacatt ctacataaac atccggtaaa aattgcatct caattttcag
   159121 ttgcccttgg ccttgacacg cctcacaacg ccccccgtgc acattgaaag agaatcggcc
   159181 ggctttatat cccctcattt tgctttccgg aagctcagcg aacagacttc gtatttcatc
   159241 aaaaacgccc gtataagtcc ccggatttga tcggggagtc ctgccgatcg gtgactgatc
   159301 gatattgatg attttatcga gatattgagt accttcaatt ttcttatatt ttccgggagt
   159361 tgtcatcgcc cgattcagat catgtgccaa ggctttatat aatatatcat tcaacaatgt
   159421 tgattttccg cttccggaaa ctcccgtaat acatacaaac attcctaagg gaatttcaac
   159481 atcgatgttt ttaagattat tctcactcgc cccgatgatc ttcagttttc tttcatccgg
   159541 tttccgcctt tgttccggta tcggaacaaa tttccttccg gtcagataat ctgcggttag
   159601 tgaatcccga cattgcaaga cctgatttaa tggccccgat acaacaactt gtcctccttg
   159661 atctccggct ccgggtccca tatcaacaat ccaatctgcg gatcgaatgg tttccgaatc
   159721 atgctccacc acgaggactg tattccctaa atcccgtaat ttcttcaatg tctgcagtaa
   159781 ttttgcatta tcttttgcat gtaaaccgat agaaggctca tccaatacat acaatacacc
   159841 cattaattga gatccgattt gagtcgccaa ccgaatgcgt tgcgcctctc cgcccgaaag
   159901 acttgccgcg gaacgttgaa gtgtaatgta atccagaccg acatttacaa ggaaatccag
   159961 tcgtgaaata atctctttta ataccttttc cgcaatgatt ttctgacggt ccgtcaacaa
   160021 tccgggtttt tcatataaat ctttggccca ttgcaaagtc gtattgatcg acatctcggt
   160081 aacatcgatg atatttttac cgagaatcgt cactgccaaa gattccggtt tcagtcgttt
   160141 tccatgacaa acctgacagg gagtttcttt cataaattca gcgattcgat ctcgaatata
   160201 gtctgaattg gtttcattgt atcggcgttc caaattggga atcacacctt cgaatggggc
   160261 tcgataagtc gattttcgat catttcgtcc ggttaaaacc atattcaagc tggctccatt
   160321 ggaaccgtat aaaatgattt tcattttatc tttcggaatc tttgaaacgg gcatatccaa
   160381 atcaatatcg tatgccgaag cgacttgtag aagctgttgc caataataac cgttatcaaa
   160441 tcttgaatta ttccattcca aaacagcgat tgcgccctca ttaatcgatt tcgaattatc
   160501 gggaataatc agattcggat caatctccag ccgcattccc agcccttgac aagccggaca
   160561 tgcaccatgg ggcgtgttaa acgagaaaga tctcggttct atttccggaa tgctaatccc
   160621 atgttccggg caagatagaa actccgaaaa atacatgtct tgtgccggct ccgaagagag
   160681 gttggtgacg gttaaataac cttcgccgac ctttaatgct gtctctaccg aatcggccaa
   160741 ccttgaccgg ttcatttttt cctcttccgg atcttcaaaa tgtcggataa tcacccgatc
   160801 aactaccgct tcgatcgtgt gaatcttata tcgatccagt tgaacttcct catccaatga
   160861 ataaaccgtc ccgtcgatcc gcacccgaac aaaaccgctt tttctgatct cttccaaaac
   160921 agcttgataa gttccctttc ttgcacgaac aatcggagca agaatcatca aacggctccc
   160981 ttccggcagc ttttgaatag cttcgacaat ttcatcggag gattgtttgc tcacttctct
   161041 tccgcagatc ggacagtgcg gaatgccgat ccttgcataa agcaaacgaa ggtaatcata
   161101 aacttccgta atcgttccca cggttgaacg cggatttttc gaagttcctt tttgatcgat
   161161 cgaaactgcc ggtgataagc cttcaatcgc atcaacatcc ggtttatcca tctggcctaa
   161221 aaattgtctg gcataagcgg ataaagattc cacatatcgc ctttgccctt cggcaaaaat
   161281 agtatcaaaa gctaacgaag attttcccga tccggaaaga ccggtaatca caaccaattt
   161341 gtctctgggt atatcaactg aaatattttt taaattattt tcccgtgcgc caagcacacg
   161401 gatcacattt ttatttgaca caaacaaact cccatgaatc agcccaacga ttgcagggct
   161461 gctctgattt tatcttctac ggtatccgga gtatccttag ggacagattg gacagcggtt
   161521 tgcgcttgta ccaaactgta acctaatgct gtcagcgctt caatcgcctc taaatctgcg
   161581 gaatgaagat tgctcaaggt tgcattttcg aaagaatcgg atattttccc ttgtaaatat
   161641 aaaacaactt tttgtgcggt ttttttcccc actcccggaa ctctcgaaag aaaatccggt
   161701 tgatcctgaa tcactgcacg tctgacggta tcaactccca ctgtcgagag aattcccaaa
   161761 gctaatcgtg gcccgattcc gctgacaccc atcaataaag agaaatattc aacttcttct
   161821 tttgtttcaa atccgtataa tgccaataaa tcttcacgaa cgattaattg tgtatgaaga
   161881 agaatttctt tccctttttc ggcgataccg agcaagctct gcggaacata aactttcaat
   161941 ccgatcccat tcaccatgac agtaattgaa tcggattctt tttcaattac ggtgccttcc
   162001 aaatacgaaa tcatgcattt cccttctctg aggttaacac ataccctcga tggcaattat
   162061 atatttgata tagcatgata cccgtcgcga cagctaaatt caatgaatcg ctggatctta
   162121 acatcggaat acgagccata aaatcacaag aatcgatttg ttctcgagtc agaccttgtc
   162181 tttcgcttcc cattaataaa attatcggat tcggataaga aatttccaaa taatccgttt
   162241 cggtatgatc cgaggttcca accattttta tttttttttc atttttccaa gccaaaaaag
   162301 tttcgaaatc cgtttgaatg atcttctgag aaaacaaagc tcccatcgac ccccggaccg
   162361 atcccgattc gtacggattg accgaatcac ccagcagaac aatcccattc gcgtcaaccg
   162421 catccgatgt ccgaataatt gttccgagat tcccgggatc ggctatttcg gttaatgcca
   162481 cgatgattcc tttttttgta ttcaaatcat ccaaatgata ttctttcttt atgccgattg
   162541 ctgcgatccc ttgcggattc tctttaattg aaaacgattt aaaaacactc tcactgactt
   162601 ctaagatttc caaccctgtt tgttttccct gttcaattaa ttgattcccg tattcacttt
   162661 ttaataattc cggacaaaaa atgaccgttt ccaacaagtt ttccgtttta tatgcttctc
   162721 cgataattcg aagcccttcc atataaaaca ggttggattt tttttgttgc tttttatcct
   162781 gaatttggcg gatttgtttt atttttgaat tggaagtact tgttatcatc atcagaaaga
   162841 ttcgagtata ttcggaaatc gattcgtatt cgcacaacaa attgcgattg ccagagcatc
   162901 agccgcatca tccggtttcg gcatttcatt aagtttcaga attgctttaa ccatcatttg
   162961 gacttgacgt ttctctgcgc caccgtaacc gacaaccgct tgcttcactt ccatcggagt
   163021 aaattcatca acatctatgc cggctcgtgc caaacttaga aggataacgc ctctggcttg
   163081 tccgacactg attgcggtag taacattgct gtgaaaaaac aacttctcta cggcacccct
   163141 ttgaggtcca tagagaagta ttttttcatt gatttctgaa taaagttgca ggagtctgtt
   163201 ttccatctta tctttcgccg gtgtgcaaat aaccccgtaa tcgattacgg ataattcacc
   163261 tctgtcagat aagtcaataa agccatatcc tgtcaatgcc gtgccaggat cgacacctat
   163321 gataaccatg caactattat tcttcgtttt ctaatgctgc aattgcctca tcactgatat
   163381 tcatattatg atagacttgt tgaacatcat ccaattcttc caatgcatca atgacacgaa
   163441 gtacctgtaa tgtatcatcc agaggaagat cgatcggttg attcggtatc atgcgaattt
   163501 gcgcttcttg aatatgcaag cccgccgcaa ataaagcatc gctcatcatt ttataggcat
   163561 cagcttccgc ataaatttcg ataccgtctt cactttgttc gacatcatcc gcaccgtttt
   163621 caagcgcgat ttcaaaaatc ttatcgaaat cgagattatc tccggtcaga agaaaatatg
   163681 cttttcgcga aaattgccag gcaacggatc ccgcctcacc taaattacca cctgctcgag
   163741 acaaagcatg tcgaacatcg gcaacggttc ggttcctatt ctctgtcaca cattcaacca
   163801 taaaagcaac gcctttcggt ccgtaacctt cgtacattac atcttcaaaa actgcgccgt
   163861 cttttgattc tcccgtaccg cgttttatcg ctcgctcgat actgtctttc ggcatattac
   163921 tttcccgcgc cttatcgaca gcgagccgca gtttgaagtt catattcgga tctccaccct
   163981 cgcgcgctgc catggttatt tctttcgcaa gcttcgtaaa aacctggccg cgcttcgcat
   164041 ctcccgcacc tttctttcgt tttatcgttg cccaatgaga atggcctgac atattttcat
   164101 tccccttttc tgaatggaat cattctttat gacagaatgt tttttaattt cgatcgttca
   164161 ttttgctttt tatctaatat acaaatgact aaataattat acacttatta tgagttgcgt
   164221 cgatatcctc gcttcaccaa acatgaactt ttatccatta aatccggcct gataccgatt
   164281 atttcgtttg ctttaccacc ggattgacta atctgccgat tccttcgatt tgagtttcga
   164341 gtatatcccc ttccttcaga ggagaagtcc cggccggcgt cccggttaaa ataacatcgc
   164401 ccggatttaa agtcataatg gatgaaatat aagcgataat tgcaggaatc gaaaaaacca
   164461 tatcacgggt tgaagccatt tgtctcagtt cggaattaac acgacaggtt aacatagcat
   164521 ccgccggttc aaaatccgtt tcaatccatg gccctaacgg acaaaaagta tcgtatcctt
   164581 tactgcgagt ccaagtatca tctttctttt gtaaatctcg agctgtcaca tcgtttgcaa
   164641 cggtgtatcc caagatatag gaaaaagctt cttccggcaa caccattttc gcttttttcc
   164701 cgatcactaa agccaactcg cattcatgtt ccactcgctc ggaagtcggc ggaaggacaa
   164761 tcccgtcacc ggtaccgatt actgcactgg gaggttttag aaaaatagtc ggatactcag
   164821 ggacaggccg atcaaattcc tttgcatgat cttgataatt ccaacccacc gccacaattt
   164881 ttccgggaat caaaggtgca agtagtttta cacgatcaat cggaagtgtt gcttcaagac
   164941 gacgataatc ggaaaacata tcgccttcaa taccgcctac catatcttca tagatccaac
   165001 cgatttttac ttcaggcttg ttatcctgaa tataacggat aaatttcatt ctgcacccct
   165061 ttctaaaacc aattcttttt ctggaaaata taaatcgcaa aaaaagtaaa taaaacaatg
   165121 atcccaaata taataatcat ggtagattca tgcgtttcga tgacatccgg aaaattgata
   165181 ttcatgccga aaaatccgct gataacattc ggaatgctta atataatcgt tacagatgcc
   165241 aacaatttca tgaccacatt gagattgttg gaaatcaccg aagcaaacgc atccatcaag
   165301 ttggataaaa tggaagaaga gatattggtc atatccatcg cttgggtatt ttcaataata
   165361 acatcttcaa ttaaatcttc atcttcttca tattttttga atgaagaaag ttttgaaatg
   165421 cggtagagag ccaattcatt ttgtttcaaa gcttgcgcaa aataaacata gctcttttga
   165481 tattttaaca tttcaagaag ttctgtattc tgcatagaag catacaattt gtcttctaat
   165541 gcatcgaccg ttttattaat cgcccttaaa tcatcgagga aatttgtagt tcccctcatt
   165601 aaaatacgta gtgcaaatcg attccttttg gtggtcgaaa gttttggtgc tttcccgctg
   165661 gcaaacattt caattgtttc ggtcgatttt tcacagaccg taacgatata tttatccgtt
   165721 aatataatgc ccaaaggaac cgtaatataa gggatatcga cattctctcc cataaaatag
   165781 ggaatacgaa caacaataag agttgttccg ttatcttctt cctcgattct ggagcgctca
   165841 tcaatatcca aagaataggt gatgaagtcc atcggaatac cgagattaca tagtaattgt
   165901 ttttcatcgg gagtcggatt gatcgcatcg atccagcagt tggtaacagg ttcatccagt
   165961 ttttccaata tcaattcatt atttttataa tatctgagca tatctcacct ctgccgatcg
   166021 ttatttgtca tatgaccgct taattttact aatgagatgc attttcgaac agacccgatt
   166081 tatttttgta gtgatttttg cggttccatt tctgataaac ttcatatgga aattccgaag
   166141 gaaagagcta tagaccatgc tataatttaa ttgcaaacgg ggatgccttg gtttcgacgg
   166201 gggaggctgg tcccgaattg cgagccgaga ggtaccgatc tcgtaaaatc agtgcaaaaa
   166261 attaagtgcc aataatcgca cttcctacgc tgcaatgcct ctcgctgcat aagcgataag
   166321 ctagtagctt tgaccttttc aggtcaacgt ttgcctgcat cgtattcccg accgatcgca
   166381 gagcaggcgt aacaaagccg ggatgctgac catgaatttg cggcatggtt caaaagaggg
   166441 ctttcgggcc cgagcaaata gtcttgatcg gttttgttca tttttccgat agagacgaaa
   166501 taaataaatg aactacgctc gtagtatatt cgaggatcga ttctttcgga cgcgggttcg
   166561 actcccgcca tctccactaa aagattgctt atgttatggc aatctttttt ttgaattcaa
   166621 tattaatact attgtgattc attattaatt ttataattga ttttgcatat catcgtaata
   166681 aaattctaac ttgataattt gcttatttta ctcgtaaaat aagcattttt ttcgcaattt
   166741 taatcttgaa agattaatta tattaatata gatttttctg ttttataaaa ttcagattgg
   166801 ctaaacattg acattcctat ataaatgttt ataatatttt taacacgttt tatatcgttt
   166861 tttgatgaat ttgttatagc gggaaaaaga aaaataataa ttgattagat tcttttagag
   166921 ctttttgtgg agaatggtcg ttgttacatc agcttgtgaa aggagggaac aaaaacaaag
   166981 atcaaggtag cttcttgttg tcttgtattt gcgtaaaatc tgattagcca tatattttaa
   167041 aatttttgat aaggagattt aattatgtcg aaaaagataa atttaattct tactttactc
   167101 gttatagtag gaatgcttgc tgcctttatt cctgcttccg cgcaggactc agatgtaatt
   167161 aagattggaa ttgtcgatag tatgacgggt ggtcatgccg cctacggact tgcttcattg
   167221 gatggttata aactggccca taaatatcgc gatacagcgc ttggaaagaa gattgaattc
   167281 atcatcgtgg atacaaaaag cgataaagca gaaactgctt tggcgacttc cagagcgatt
   167341 gcagatggtg ctgtggcttt gttgggaaca gcctcgagtg gttttaccat tgcgatgaat
   167401 gaagttgctg ccgaaaaagg aataccgacg gttacaaact tttcaacgag cccgttggtg
   167461 actcagggaa atgatattgc tttccgttct tgctttatcg atccttacca agggtgggct
   167521 ttagccaaat ttgccattga aaatctcgga gcgaagactt ctgtattact ggtcgatatt
   167581 tcgactgact atccggtggg ggtggcaaac tatttccgcg aaacgtggaa tgaaatgacc
   167641 ggcgattcca aaaccctctt aggatattat tcgattatgt tgggtgaccg tgatttcacc
   167701 gcacagttga ccgctatcaa acaattaaat ccggatgtag tcgtagttcc agacgattat
   167761 gccgaagtcg gtttgattct caaacaggca cgggaattgg ggatgacaat gccattttta
   167821 ggcggagatg cggttgacct tcccgaaatc gagggaattg caggcgacgc tattaaaaat
   167881 gatttttatt tttcaactca ttggcatccg gatgcatttc aaaacgaagc atcaaaggct
   167941 tttggcgatt attaccggaa agaatataat cgagaaccag acgccgcggc tgcgacagcc
   168001 tgggatgcat acaatatgtt gattgatgcg atcgaacgtg ccggatctac cgacccgact
   168061 gccattaaag aagcgttgta caagacggat atggaagggg tgaccggcca tatcaagatt
   168121 gatgaaaatg gcgacgcgat caaaccggta acgattctcg gatataaaga tggtgtcaag
   168181 aagataattg caatcattca accggaagag aaaaaataaa ataaattctc atttgaaaac
   168241 tatttggggc gattttatat gccccaaata gttttttgaa cgttttcttt ttatattttt
   168301 taataaatag ttttgttgtc attcttgaat atttgcagaa ggtaactcat ttttcgttta
   168361 tccgtttttt tgagttattt atcctgaacc cggaagaagg tatttttaac atgacatttg
   168421 ataattttat ccagttcgta gtcatggggc tatcactggg agcgatgtat tctttaattt
   168481 caatcggata taccataatt tatggtattt taaaattaat caatctcgca cacggtgatt
   168541 tttttatgtt ggcaggtttt ctgggaatgt ggggagcgct atattacggt gttccattgt
   168601 ggctctctct tgttttcggt ctggcactga cggtcggtgt aattcttttt gtcaattggt
   168661 ttatttatcg ccctttacga cagtataaga tggctgcctt tacaagtact tttgcgattt
   168721 cgatgattgt gcaagcatcg gttatcatgt ttctttccgc acgtgctcaa gcgtttccgc
   168781 atcctgattt cttaaacaca ccaatcaaaa tcggcaatat tatcattccg atggtaacac
   168841 cgtttattat tgtgattgct ctcattttat tcggaatcct tactttaatt gttaataaaa
   168901 ccaaaattgg gcgggctatg agagctgtca gtctggatat ggaaatggtt acgctgatgg
   168961 gcgttaatgt cgaaaatgtg attgcctttt catttgcctt gagcgttgtt tatgccggaa
   169021 tgggtgctta tctttgggga atgcgctatc cggccttcga tccgaatatc ggtgtcgtca
   169081 ccggtctcaa ggggtttatc ggagccgtca ttggaggaat cggaagtatc cccggtgcga
   169141 tgctcggtgg atttttactg ggatttgcag aaattatgct gatcggattt ttccctcagt
   169201 ataccaattt tcgggatata ttttcctatg cgatcatgat tattttcttg cttttccgcc
   169261 ccggtggaat tttcaatgtt aagatccgcg aagagaaggt ataaaatgat caaacagaag
   169321 ccgaaaaaca ctattttatc catcattgca attgctatcc ttcttgtaat attagaactg
   169381 atagggcgta gcaaaattga tcactattat atacgcattc ttcaatactg gagcatttat
   169441 atcatctacg gtgcttcgtt ccaacttctc tatggttata ccggcctttt atctttggga
   169501 catgcggggt taatcgccat cggtgcttat gcggtcggca tttttacaat cagcccggcg
   169561 acaaagacaa tgtcgtatat acttcaacca ccggctcctt ttatcagcaa cgtacaaatt
   169621 cctttcatac cggctttaat catcggcggt gttttcgcgg cacttttcgg attggtcgta
   169681 gctgccccgg cccttcgttt gggaggagat taccttgcaa tcgtaacaat gggctttgcg
   169741 gaagttatac gccttttgat tgtcaatatg ccgtccattt tcaacggcgc gatgggattg
   169801 cgcgctattc cgcaatccgc tacattatta atgacttggt cggttgcaat tgtctgtctg
   169861 tttgtcatga aacgttttga aaacagcact tatggtcggg ctttattggc aattaatgaa
   169921 gatgaaatcg gagctgaagc attaggaatt aatgtattca gcaataaggt caaagctttt
   169981 gtcttatcat catttttcgc aggcatcggc ggcggcttga tggcaagtgt attgggtacg
   170041 attgatccca atacctttaa atccacttta acctatacag ctgtcacaat atgtgtgctg
   170101 gggggaattc gcagtgctac cggtgtcatt atcgccagcg gaatttattc gattatgtcc
   170161 gaatttcttc gcgtttttga atctccgata aaaatcggaa acttcaattt cagcggaatg
   170221 ccgggcatgc gtgttttggt ttttggaatc atgcttctat tcttaatgct tttcagaaga
   170281 gagggcttat taggttccaa agaattttct tgggattggt ttttctcact ctttaaaaag
   170341 aaacctccca aagagcaaga gaaaactgtc cacaccgaca aaatgggagg atcgttatga
   170401 ctcccattct tgaagcaaag aatttgacca tgcgattcgg cggactaacc tctgtatccg
   170461 atttcaattt gacgatggag aaaggggaac tggtcggatt gattggtcct aacggcgcgg
   170521 gaaaaagtac cgtatttaat atgatctgcg gattttataa gccgacgatc ggagaaatca
   170581 tttttgacgg gaagaacata accggcatga aaccataccg aatcgcaaat atggggctaa
   170641 ttcgtgtttt tcaaaacggc aggctgttca aaaatatgaa cgttattgaa aacatgatgg
   170701 tcagtaagca tatgcgtttt aactcaaatt ccctgaaagc cattcttcgc actccatccc
   170761 acaaccgcga agaacagagg gtttttgatg aatctgttgc cctgttggaa agaatcggtc
   170821 tgaaggaaat gatgtatgaa ccgaccggaa aacttccgtt cggtattcaa cgaaaattgg
   170881 aagtcgctcg tgcattatgt gcaaatccga gtttactatt actggatgaa ccggtagccg
   170941 gattaaatgt cgaagaaaca aacgatatga tgaatttcgt tgatcaactg agaaacgaat
   171001 tcggtataac cattcttttg atcgaacata caatgagagt tgttatgtcc ctttgcccta
   171061 aaataattgt tattgatcat ggcgagacga ttgcggttgg tatccctgat gaaattcgta
   171121 ataataaaag agtaatcgaa gcatacctgg gggtggatga acaatgatac aaattgaaga
   171181 attaaatgta cggtacggtg gcattcatgc tttaaaggga atttcttttg aagttccgga
   171241 taattcaatt gtcacccttc ttggcgcaaa tggcgccgga aaaacatcca cactcagatc
   171301 catttgtggc ttggtaaagc cacaatccgg ccatatttat tacggagaag ataagaagga
   171361 tttaacaaaa atggaagcag ctgctattgt ccgcgccgga atctcgatga taccggaagg
   171421 aaggcgcgtt tttaccgggt tgacagtaga agaaaacctc caaatgggag cttggaaccg
   171481 aaatgacaaa gacggaatac aagaggacat cgatgttatc tttcatttgt tcccggtttt
   171541 aaaggatcgc tttcatcaaa aagcactcac gctctccggc ggagaacagc aaatgctatc
   171601 cgtcagtcgc gcattaatgt ctcgtcccaa gctccttctg atggacgaac cttctttggg
   171661 attggctcct ttgctcgttt cacagttatt taaaaccatt ttggaaattc ataagagcca
   171721 aaatatgact attcttttgg ttgaacaaaa tatgcgagcg gctttaaaaa tagcggatta
   171781 tgggtatgta ttggaaacgg gagaaatagt ctttcaagga acgccggagg aactttctga
   171841 taatgagaaa gttcaggaag catatatcgg ataaataacg catttaatta tccgatcgca
   171901 gtaggagtgc catatgtttg atgtccataa agcagcccgg agtaccatcc gggctgttca
   171961 tgaatatgtt ccgaataaac ctcccgattc tatcatgaaa gaaatgggaa tcgagcattt
   172021 tgtcgacttg gctaccaatg aaaaccctta cgggatttca ccgttggcat ataaagcgat
   172081 tcttcgagaa tcaaaatcat ctttaaatca ttatcccgaa agtacctgct ttgaacttcg
   172141 gaaaaagctt tcggaaatcc actgcttatc cccggattgt tttatcgtgg acaacggtct
   172201 ggattcgatc ataagattga tcggattgac tttcctggat gcaggcgatg aaattattat
   172261 cgggagttca actttttccg cgtatgagac aaccgccaaa ataatgggtg ccaaagtcaa
   172321 taaaattccg ctgacggaag attatcgttt gaatattgat ctgatgattt cagcttgcaa
   172381 ccggaacaca aaagcaattt ttatctgtaa tcctaataat ccgaccggca caattattac
   172441 aaaatcggaa tttataaaat tgatggaaaa gatttcgcca agtattcttg ttatttctga
   172501 cgaagcgttt tatgaattct ctgacagtcc tgattatccg cagacgattc cttttctttc
   172561 cgaatatcca aatctgatca ttctgcgaag tttctcaaaa acaatggggt tagccgggtt
   172621 acggatcggc tacgcaatct cttcagaaga gatcattcgg atgatgatgc gggttagaga
   172681 gcctttcccc gttaatcgat tggcacaggt tgctgcaatg gcgacgttag atgacaaaga
   172741 tttcattcaa caaactttgg agatcaatcg agacagccgg gtgaaaatgg ccaaagcaat
   172801 gcaactatta aggttgaagt gcatccccgg tcaaacaaat tttatttttg tcgacttggg
   172861 ccaaccggta gaacctatat ggaacgcgct attctcaaag ggaataattg tcagaagatt
   172921 agacggaatg aacttaccga caggtattcg aatttgcttg ggaaagcaaa aagatttaca
   172981 gttcgcgttg aatggattag ccgaaatttt aggggcgccg gcaccgctta tatttgatct
   173041 ttgaaccata aagtttctta aacttaaaag aagaaatcca tgcttaattc cacatggatc
   173101 tctttttacg atatcggctt cttttagatt tgtattatga tttgaaacac gctacacact
   173161 ttattcccat tttaaatatt caggcactac aggtccgtcc ccattcggtt cgctcttaat
   173221 gagttcaaga atagcattct tcgtgccttc caccaactta tcctgatcgg gaggagctgt
   173281 ccgtaccatc tgagccgtgg ggcttgtctt gaaccataca tctttcagac cttgccaatc
   173341 tccgacatac tcccattttt cttccggcag tttatttccc caagtatgag tttcagaatc
   173401 atcgccttcg gttgcgggtt cccctggagc atagacttcc atcgtatatg cgtgaatgcc
   173461 caatctgccg tatgcccaat caatcatctc tgaatccgtc tgataatcat gagcggattg
   173521 cttctcataa gcgggtcgtc cggtcacatc ttcgtaagcg gcaatcattt tttgagcgac
   173581 gcctttgaaa aagtcattat cttttgtcgg ttcctgagta tagcaccaag gataaaggac
   173641 actttgttcg ccggtatgta aagaaagaac ggcataaacc ggatgataaa tcataaaatt
   173701 ctgtacagct tggacttccg gttcactggc ggcatcgggc cccgccctct tatatatatc
   173761 ttccccgaga atcggatccg tatctacatc taagtaattc cacatatagt caaaagttcg
   173821 attcagatcg ataccgctgt tcttgggatc atctccgggt atgccattct tatcccaatc
   173881 gggactttcc atacccacat aaactcgatc ttcaactttt gaatcctgag cgccggtata
   173941 aatttctgaa ataaagccgt cgccgttggt atctgtataa ggatcgctga acacttttcc
   174001 gtcaccgttt gcatccctcg ggcgcaaatt ttgccgggta ttatagattg ccgaagcggc
   174061 gtatccatcc ggattcatta ccggcgttac ataaataata tatttatcca aaatatcttt
   174121 cacagcagca tcttctgaat tcgtcacaag ccaccatgca aagtacattg ccgaaatcgc
   174181 accttcattt tcaccgccat gaatattccc gacgatataa attccggtct ttggttctgt
   174241 accggtgccc gtaatttcag ccgtccataa ttgtcgctcg cgccaactgt gaccgatcgt
   174301 atatactttt acaatatccg gataattttc agccaatgcg tgcagcgtct gcccataaaa
   174361 atcaacatca tggtaaacgg accaatcaat atcatctaaa ccggccgctg cgaatcccgg
   174421 agtataaagc gtcataagca ataacaatac gataataaca aaaaggtttt tcttgttaaa
   174481 cataaaattc tcctgatcta ataaacgaac aaaaagttcc tctgaagccg atatattcat
   174541 tatagattgt gcgaaaatta gcaattagta attttcgtaa tgaaaaaata gcataaatgt
   174601 cattcttaat tattatctat aattattatt ctattattta ataaaataag aggctgatca
   174661 tcaatttgtc agcctcttat tttatgaatt cttcgattta atcgattttt attcctttac
   174721 aggcacacat tattttttcg taggtaatcg gcattttatt tagattaaga tctgttccga
   174781 ttgcatcgcg aatagcatta atgaccgccg gagcaataga attatcagac ggttcaccca
   174841 ctcctttcgc tccgtaagga ccaaatccgc caccactttc gagaataatc gtttcgatat
   174901 ccgggatatc aaccgatgtc ggaataatgt aggtagaaaa gttattcgcc ttggtaattc
   174961 cgttccgcca ttgcaaatcc tcgtgcgtcg cccataaaga atgataaaca gcaccgcctt
   175021 ccacctgacc ttcacaagag ttcttattta ccatctttcc ggcatcgatt cccgcaataa
   175081 ttcttagtat ttcgaataca ccggtttctt catcgacggc aacttctaca ccatggcagg
   175141 tgtaagtcaa atccggcaaa acttcgccct ttatgtcttt caaattcgga acatccgtaa
   175201 aaggagcatt gaactgttcg atcgtttcca acattccacc gttgttactt aaactattga
   175261 caaccgttac aaagggaaca ttaacagtcg gattattgac aagatatact ttgcgatcgc
   175321 ccaaagcaag gctttcaacc ggatatttta aaatcttgga tgcttcgttc agaatccggt
   175381 cacgcataac atcagcggct ttcatcgcag catttcccga catatagagc tgtcgggtgg
   175441 cggttgtcgt cccgcaaagc ggtgttaaat gagtatccat gataaaggga tggacatctt
   175501 caatatttaa acccaattct tctgcgacaa tttctgccaa cacacttgcc tgtccgccgc
   175561 cgatatccgg cacgcccgcg cgaagcgtca cggtaccgtc caactcaata cgaatagaca
   175621 cccgagacga atcgtgcata aaggtcaacc gaccataggt catatgtccg attgcaatcg
   175681 ctcgaccgac tttacgtcca tccggtaagc gatccggttt cggtgtcgca tttaatgatt
   175741 tatcaacttt atcaatcaat tcttccagcg cgatatgccc ttgcggtata aatcccgtcg
   175801 gcatcgcatc tccgttatgc ataatattgc gtttgcgtat ctcgacccgt gatattccca
   175861 atttatctgc caactgatcc atttgtcgtt catacgcaaa attcacttgc ggactgccga
   175921 atccgcggtt tgcactggat aaggtattgt tcgtaaaagc ggagacgctt tcgattttta
   175981 cgttcggaat tttataaggt cccgcagcgg cataagtgga gtacatttga acccagggcg
   176041 tcagataagt atatgatccg ccgtctaaca caatcgaaac ttgctgcgcg ataatttttc
   176101 cgtcttttgt agcgccggtc ttatagtgga ggtattcggg atgtcgcttc gcatgggttt
   176161 caatagactc ctctcggctc caaaccatct taaccggtcg gcgagttttt tggacaagca
   176221 aagcaatata cgtttcaact gtgatatctt cttttccgcc gaagccaccg cccatcggga
   176281 cactgatatt tcgaaccatt gtatggggga ctcccaagat tttcgcgatt gtccgataat
   176341 gttccaaaac ctgagatccg acccttaacg ttatgacacc gttttcatcc agccaaccca
   176401 tacccccttc tgtttcaaga taagcatgat cattcggtga tgtgtaataa tcattttcga
   176461 tgatgatgtc acattgatcc caaactttat caacatctcc gtcttgaatc ttggttcgca
   176521 taataacgtt gctgttatct tcgctcgaaa ccagatatgc accgtctttt atagcttccc
   176581 tcggatcaaa aacgccttca gtcggctcat aatccacttc gatcaggtta catgcttctt
   176641 cggcgatttc ctcggtctct gcggcaacca acgcaatggc ttcacttttt gatcgtattt
   176701 ttcctgtcgc caaaacttta tacaatcctt caaatccgcc gccgacatca atcatttgtc
   176761 cgaatttcgt aatatcttca ttgtgcggga catcttttgc cgtaagtaca gcttttacgc
   176821 cgggcaaagc ttccgcttta ctggtatcga ttccacgcag gatcgcagcc ggatatttgc
   176881 tccttaaaac tttcccatag agcatgttcg gcatagtcca gtcgtcactg aaaagttgct
   176941 ctcctttagc tttagctctg gcatcatgcc gagggaccgc tgttccgaca agtttcaaat
   177001 tatgtttcat gctaaagctc cttcctcaat ctcttcggca actttataaa tcgcatcgac
   177061 aatttttata tagccggtac aacggcacaa atggcctgaa atagcatatt taatcgcctt
   177121 tcgatcagct tttccgccat tcttcaaata ttccacacca gccataatca ttcccggagt
   177181 acaaaaacca cactgtaccg atccgcattc gacaaatgcc tcttgcagcc gggctgtcaa
   177241 tcgatcgttc aatcccaacc ctttatcggt ccatatttca tggccatcgc aagccgctgc
   177301 cagtgtcaaa caacttttca cttcttttcc gtctaacaga acggaacatg ttccacattc
   177361 tcccgcctcg catccgcatt taacatccca attaaaattg tttcgtaatg ctctcagaag
   177421 attttcctgc ggttcgacct cataggtgac attttcaccg ttaactttta aatttatttt
   177481 gatcttattg ctcatattcg gacccttcct ttattcgcat tcgccaaagg ctgcaagctt
   177541 tacagcccgc ttgacaagca ccggtacaac agaccgtctg taactggcgg tggcacgaac
   177601 ggatgggcta cgcggactga tatcattttg gactgcctca ccggctttcg taaaggtatc
   177661 ttcatccagc ttattcccga taaatacttt ttccgcattc gttccccgaa ccggtttcgg
   177721 cgcgactccc cctaatgcga ttctgcaatc ttttacgatt ccggattgtt tatccgtttc
   177781 gatataagcg gcaacggttg caatagaaat tgtcattgac cgccgtgctc cgagttttat
   177841 aaaaccggta ttcggttgtg attcaaatgt gaaatgagtg atcatttcat ccggttcgcg
   177901 agtggttacc ccatttttta taataaactc attaatcgga acttcccggt cccctctctt
   177961 gcttttcaaa tgaacgatcg cattcaatac catcaaagga ggaatggtat cgccgcttgc
   178021 cgatgcattg ccgacatttc cgccgatggt tgcggatcga ttggtggtag gatcggcgaa
   178081 gacattggcc gccgcgaata aacaaggtgc atgttgtttg ataatttcgg acttggcaat
   178141 ttcttcaata atcgttaacg ctccaatact gaccttcccg tcggtataag ttattttctt
   178201 caatgattca atatcacgaa tatttatcag agtgccatct tttattttct ttgcccgaat
   178261 atcaaccatg acatttgttc cgccggctac aagacaggct ttgtttcccc attgagctaa
   178321 cgcctcaatg gcagaatcaa cagtcggagc attaacaaac tcaaaatcac tcattttttt
   178381 cctctttcat tcgttgggct gcgacttgga cagcttttat cattgcggca tatccagtac
   178441 atcggcaata gtttcctctg agataatttc gaatgaaatc ctcatcggga tccggatttt
   178501 cgcgcaataa tgccgttgaa gttactacca ttcccggtgt acaaaaaccg cattgaatag
   178561 ctccctgatt aataaattct tcctgcaccg gagataattt atcgccgtct gcaagtcctt
   178621 ccgatgtggt aatttcccat ccatcggcta ccacggcggg aacaagacaa ctcataatgc
   178681 tgcgatcacc atttaaaatc gtacaggcac cgcattcagc cagaccgcac ccgtattttg
   178741 tcccggtcaa atatagccgg tcccgcaata catttaatag caactcatta ctttttacga
   178801 ctaatttatg agttttgccg tttactttca aggttatttc atgcagactc atgataattt
   178861 tcctccgctt cgttcccaag cttgctccaa agcatctctg gaaataattt ttgatgtttc
   178921 aatacggtat gcggcagttg ttcgatttcc gccatctcgt ggtttgatcg cttttgatat
   178981 tttttccgcc gtctcaataa tgagctcttt cgttaatgtt ttcccgacca gtccatcacc
   179041 gatttctttc aggaataacg gtttgccggc aacggatccc atcgcaatcc ggcaagatcc
   179101 gacaagattg tttt
//
LOCUS       DAOSMM010000002        72171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_384_length_72171_cov_17.362097, whole genome shotgun sequence.
ACCESSION   DAOSMM010000002 DAOSMM010000000
VERSION     DAOSMM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 72171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72171
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_72171_cov_17.362097"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1315)
                     /gene="mgrA"
                     /locus_tag="PK898_00845"
     CDS             complement(266..1315)
                     /gene="mgrA"
                     /locus_tag="PK898_00845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HOU18608.1"
                     /translation="MIDAHHPSLTSVYAADPARYDNMIYRRCGKSGVQLPILTLGLWH
                     NFDGYKSFETDRAMIRYAFDHGITHFDIANNYGPPYGAAERVFGQIYEQDLKPYRDEL
                     FISTKAGYDMWPGPYGDYGSKKYLVASLNQSLKRMGLDYVDVFYHHRPDPETPLEETM
                     GALDFLVRQGKALYVGISNYPADLTKKAAEILRELGTPCLIHQPSYSILNRWTETDHL
                     FDITAEYGMGTIVFAPLQHGLLSSRYLNGIPEGSRASLDRHLKKDLITDESIGYIRQL
                     NSIAIERGQTLSQMALAWVLKDNRVTSALFGASNVEQIAENLRVMENLSFTAEELQEI
                     DAVANPFMEFLKRHS"
     gene            complement(1312..3321)
                     /locus_tag="PK898_00850"
     CDS             complement(1312..3321)
                     /locus_tag="PK898_00850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU18609.1"
                     /translation="MALLYPVLSILESLFAGLLLWKPAESGGISRLRLIFMILIALIV
                     VVVLSGWLFRSKILRRSEKIGTACFFLSAACFFIAFTTRFYFDNPALDFTVAYLKQAL
                     PFILLTAILSLQTFIGIVYYYSKENGSVSRAFKQLFWGGRIVWQVVLIFLACLTLMSA
                     FIPIRANYYPSFDSSIFSYIGQQILRGKLPYVDGWDHKPPLIFYIDAFGLWLANGHLI
                     GIWLLEVIALLLGSILFFRILKKYFSEGIALSVTMLGILHQARLFDFGNYTEEFSLFF
                     QLAAFGLAFSDRFRNRLGWQGFLSGVLCGLAFTCKQNTVGLWAALFLLDILQLYLNRD
                     QKSTFVHQVIYKWVRFLIGFLAVNGIWIVYFAVKGILREYWDVAFVYNFVYAQKSGES
                     RWATGLTTLTFLPSLSVFLLLAFLSWPAALFDRIRSLRQCNRNDRQKLFDQNRLLLFA
                     LIALPVELILAGLSGMNYQHYFILCVPSACILAAWLGDSMLRQLSVRMKPVAASLLVL
                     AVFWIGSAPMANVYRRNYEPRMPSAATKTADFLNSNTDDGDLVQLWGGVLAPYVMSER
                     SSPSRFFNVRPLYLFPGYMQEEQWGTFLADLKNSPPKFIVYMNDRFLAQVPYNSNGFC
                     EDLDLADYQRPTYQFLCDHYRYRETINEGMNDAWGVFERTGKETK"
     gene            complement(3331..4833)
                     /locus_tag="PK898_00855"
     CDS             complement(3331..4833)
                     /locus_tag="PK898_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18610.1"
                     /translation="MDQTNQTKTIMISADHGLAIVYFLQTDVVKTLLEKGFRVVILTD
                     DGVTEKMRERFQSSNLFFEGLRLKDAKIYFETVDHNQQYWIHFLRWMGGSDQVNTHAI
                     DSHLRQMAFETSRRGKLLMPFIKTYIRKLRKSADARKRLVRKQNRYTTNLYGDLFDRY
                     QPNLVIASTAGWRLDRYLLREAAARGIETAFAVIGWDNPSSYRLPGAPVQWVNCWSEI
                     QKQELTLGADWLPERVHVGGIPTYDGYFRKIWRLSRETYFKYHNLDPDRKLISFACSF
                     VTFSPNYQDIEALVNIISQEKLSYPAQLLIRLHPNHFIPGSLFEEEAERIRRLIKDKP
                     YIHLVEPVALGGDLGFYSGEDMPEKDSMMAWSDVFCTVYSTMVVETAIHDRPIISVCI
                     DAPGGWKKPGKFSLSLTEIGEWPTHERFRAAKAGKVVYNEEQLTEALNFYLAHPEADS
                     AERKQFVSDECTFTDGTAGIRTAEYFAAVAENARRHSELIYHFDPLPPAD"
     gene            complement(4912..5871)
                     /locus_tag="PK898_00860"
     CDS             complement(4912..5871)
                     /locus_tag="PK898_00860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOU18611.1"
                     /translation="MRIGQNPAKFVNNVAKPARVTVAILNYIPFISGFYAELPQVLHA
                     CLDSIAESTADVEYDLMVFDNGSCPEVKDLLRDELEQGRIRYLFLSEKNLGKGGAWNI
                     ILNGAPGEIIAYADNDCLFSPGWLSKSIEILETYPNVGMVTARPFRTNPQFYTATLDW
                     AEKTEDVKLESGQFVPFEVFREFDLSLGQTEEEIRSHYEATCDHLLTYHGVQAIAGAS
                     HWQFTAYKKVLSEFLPFQMDRPMGQVKELDLRMNEKGLLRLMPVQPYVMNMSNTLADA
                     VQEKARSKKTAERNRLLDMPPVKKTLLGLYNQIFRWYYDDRQK"
     gene            complement(5951..6868)
                     /locus_tag="PK898_00865"
     CDS             complement(5951..6868)
                     /locus_tag="PK898_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18612.1"
                     /translation="MTTMTIFISPKPFNREHIATIQWNAIQSWKQLGEDVDIVLIGED
                     IGVREAAEELGVRYIAEVKRNESGTPLLSDIFALARDTSDSPLLAYVNADIILFPDFL
                     EQARRMMEKEKKFLLVGQRWDLDIPRKIDFSNNWQGKLRKELADRGRRHPAGGSDYFI
                     FPRTCFEKIPDFAIGRSGWDNWMFYEARKQGWKLIDCTKAIDIIHQDHDYAHLPNGQS
                     HYRLPESKHNVELAGGKRTIFTLLDCDWQLESNGNLVRPPRTKKKILREMEIFPLIKL
                     KSRVLGEVGFAVFHPEKAYREFRSRKKKD"
     gene            complement(6865..7641)
                     /locus_tag="PK898_00870"
     CDS             complement(6865..7641)
                     /locus_tag="PK898_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOU18613.1"
                     /translation="MKTTLIIQARMGSSRLPGKVMKNLSGKPMLEWVIKRGEQAKKID
                     ETVVATTTDPSDDAVADWCEVNSVPCYRGSTYDVLDRYYQTALWRGTDRIVRVTADCP
                     LIDPNVIDDVVSLYETTGADFAANRLPPPWHRTFPIGLDVEVVSFPLLERAWKEADKP
                     FEREHVMPWFYTPEGRFKVAILDHDPDCGMHRWTADTPDDFELLQEIFRRLPDPLHAS
                     WTEVLELVERFPELEQINETTHAKQVDAIDERILRDKKSL"
     gene            complement(7645..8778)
                     /locus_tag="PK898_00875"
     CDS             complement(7645..8778)
                     /locus_tag="PK898_00875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU18614.1"
                     /translation="MKILYFSRDYCPHDYRFLKAIHDIGHEVFFLRLENSGRNLESRE
                     LPEAVHEAEWRWREIPYDPRNADAILSDLKEIFDQIQPDVIHSGPLTDVSWLTAEADL
                     HPHAAMSWGFDLMHDIEADPRLKQNAAIALHQADWFLGDCYVERDTAIPLGLKKDHAT
                     IFPWGIDPKQFCKGNSTVRRELAEEDEFLLLSTRSLEPNYRVDITLSAFIAAAAKEPK
                     LKLAILADGSQFNLLRNIADAAPKEIRDRILWLGRKPNDQLIDYYRAADLYLSASITD
                     GSSVSLLEAMACEIPVLVSDIPGNLEWVEDGRTGFLFKTGDSAQMADKILFCYRNRGE
                     MQNITAPARRLIEEKADWEKNKYRLTEAYRNAVTVCNSRYDSK"
     gene            complement(8780..9847)
                     /locus_tag="PK898_00880"
     CDS             complement(8780..9847)
                     /locus_tag="PK898_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOU18615.1"
                     /translation="MELKFGNQTVGENHPVYFIADIAANHDGDLNRAKMLIHLAKESG
                     ADAAKFQNFKAPKIVSDFGFKNLGGQKSHQAKWQKSVFEVYQDASVPDRWSEELKETC
                     EKEGILYFSSPYDFESIDWLDRYIPVYKIGSGEIDWLESLERIASKQKPVLLATGAAD
                     MSDVTRAVETILKINPQLVLMQCNTNYTASLENFKYIHLRVLETYHRLWPDLILGLSD
                     HTPGHATVLGAVALGAKVIEKHFTDDNERIGPDHKFAMNPKSWEEMVVNTRQLEAALG
                     SPEKKINENEKETAVIQRRCLRAARDIKAGERLTRDMIDVLRPATPGALKPYEISAVI
                     GKKVLSDLPYGKEITRQDLED"
     gene            complement(9859..10794)
                     /locus_tag="PK898_00885"
     CDS             complement(9859..10794)
                     /locus_tag="PK898_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU18616.1"
                     /translation="MKKLLVTGASGLLGMNLALGLSKKYQIFGVANHLTLQGIPFEMV
                     QRDFFRKDVAAELIDTVQPDAVIHCAAMANVDACEKNPEAAMELNGEFPGRLAGETAK
                     RHIPMIHISTDAVFDGKERKENGYTENDLPHPINVYARSKLLGEQKVFEANPDAVVAR
                     VNFYGWSVSGNRSLSEFFYANLKSGQRVFGFTDVFFCPLYVWDLAEILEKLLLKNAKG
                     LYHVFSPEFLSKYEFGVNIAGKFGFDPNLIQPVSWQAGGLDAPRSPYLIMNTDKLREF
                     LGESLPDQETCLNHFYEAEKSGLPELIRSFRTDNS"
     gene            complement(10797..11801)
                     /gene="pseB"
                     /locus_tag="PK898_00890"
     CDS             complement(10797..11801)
                     /gene="pseB"
                     /locus_tag="PK898_00890"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOU18617.1"
                     /translation="MDWTDKVVLITGGTGSFGKKFIQVMLKEYHPEKIIIYSRDELKQ
                     YEMQNSGINDPRLRYFIGDVRDLDRMKRAFQGVDIVVHAAALKQVPACEYNPFEAVKT
                     NILGTENVIDAALDMGVKKVLAISTDKAVNPVNLYGVTKLAAEKLVIQSNSYAGGRET
                     RLSCVRYGNVVGSRGSVVPVFLKQRATGRLTVTDERMTRFWISLEQGVHFVIRCIEQM
                     HGGEVFVPKIPSMKVKDLAAAVAPEAEITYTGIRPGEKLHEVLISEDEARNTVEFDDM
                     FIVQPSGTTWFGRDWEKLGKKLPDDYHYSSNNNTEWLDLEGIKKMIAPFMESYDQEEF
                     "
     gene            complement(11884..12753)
                     /locus_tag="PK898_00895"
     CDS             complement(11884..12753)
                     /locus_tag="PK898_00895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU18618.1"
                     /translation="MSGSAPKNEVRHFYDEIGWKLESDGFYQNARYEDLRPVSADYIH
                     RCHLRLVPYFQNGGKYFLDAGSGPIQYREYLAYSEKFEFRVCLDISITALKEARKRIG
                     DHGLFVVGDVANLPFSSEVFAGAVSLHTLHHLPLEDQKKAWSEINRVLLPGRKAVVVN
                     GWTESAMMKHWQKRVLRAEKIGHRIARIRGKENGKTRQEKSEKAAETPTGTYVQKLDA
                     NWIRSELPAVIGRSKIEIRCWRSVNVRWLRALIHNPYGKAALRHLFALEEKKPEYYGE
                     YGQYPMIILTKEN"
     gene            complement(12750..14381)
                     /locus_tag="PK898_00900"
     CDS             complement(12750..14381)
                     /locus_tag="PK898_00900"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18619.1"
                     /translation="MQSNYKQTISKILGEIPLTAEFYFQLKKQSEPGTRYSLKNIQAR
                     LPQMLADIDQFKTPNAHPEKILVFTSLHYWIEEAATIALYFAAQGHHVTFGYLPYSDW
                     QNDVNKFDLRRQDLYTKKVFEQAAEYMDIVSFLPIHPSYKPIPDELKQIIEEVSRYDA
                     QYTLQVEDVDKEHPLYKMRLERNESFARMLYPWLSANKPDHMIIPNGTIQEMGIAYRM
                     AKWLKIPVVTYEFGEQRKRIWIAQNREVMRQETDDLWEVRKNIPVETEELKKVKAMFS
                     ARQHADIWENFTRRWQGSDRVGTEKIKQELKLDDRPIVFMATNVLGDSLTLGRQTFSR
                     NMAEWIERTVQYFVERTDSQLVIRVHPGEQLTHGTSMVDVVHDLLPRLPEHIHLITPT
                     DKTNSYDLVDVATVGLVYTTTMGLEMAMNGVPVIVAGQTHYRGRGFTYDPDNWVNYYK
                     ILGSILDDPQKSILTEEQVKLAWTYAYTFFFDYPRPFPWHLVKMWDDYDKRPFSYVLS
                     KEGQAEYKSSFDYLIGKPMDWKKILAEKKNETDVE"
     gene            complement(14381..15649)
                     /locus_tag="PK898_00905"
     CDS             complement(14381..15649)
                     /locus_tag="PK898_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18620.1"
                     /translation="MNDYAVEVHDIGKRYRIGAAPAAYRTFRETLTEPFNKSKSKHHG
                     KMTDDNSFWALKDISFNVKQGQALGIIGRNGAGKSTLLKILSRVTEPTVGSASIKGRV
                     GSLLEVGTGFHPELTGRENIYLNGAILGMRRTEIDRKLDEIIAFSEVEEFIDTPVKRY
                     SSGMYLRLAFAVAAHLEPEVLVVDEVLAVGDAEFQRKCLGKMTDVTNEGRTILFVSHN
                     MSAILRLTNETIVLEKGKMVMRAPSVEAVDYYLSHGFSQGGERFWTAEEISKDAAPFT
                     PLAIRILTDRNHVSETVRSTEPFQVEFEYRLDAPITGLRVGIYLISTRGEFIFTSFDV
                     DDPEKFTQYGTREKGKYRSRCTIPADLLNEGKYVIGVNASTFKIKRYFHEEQALTFNV
                     DGTGAPGTQWPETRSGMIRPRLNWEIEGID"
     gene            complement(15661..16509)
                     /locus_tag="PK898_00910"
     CDS             complement(15661..16509)
                     /locus_tag="PK898_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18621.1"
                     /translation="MGLETSPEDVRVTIKPSNGWKIIDFKELKHYKELIFFMTWRDLK
                     VRYKQTILGAAWAIIQPVMTMVVFSIFFGNLAKVPSDGIPYPLFSLAGLLPWQLFENS
                     LRNASKSLVANRNMITKIYFPRIVLPLSSVLACIVDFFVALPILIGFMIYYQYSPNIY
                     ALLIPYYLLLTVITAFGVSLWFSAMDVMYRDIGYIVPFISQLWMYLTPIAYGSGIISN
                     PKLQFIYNLNPMVGVVNGFRSSILGEYIKLPIQSMILSAVISILILVSGLFYFRRMEK
                     QFADVI"
     gene            complement(16525..17490)
                     /locus_tag="PK898_00915"
     CDS             complement(16525..17490)
                     /locus_tag="PK898_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOU18622.1"
                     /translation="MTEPSPLANKRICVTGGAGFLGSFIQESLKKRGVTDIFIPLRKQ
                     YDLIEVSDIRRMLSDAKPDVIIHLAANVGGIGANMEHPAEFFYDNLMMGVQLIHESWK
                     AGVEKFVAIGTICAYPKYTPIPFKEDDLWNGYPEETNAPYGLAKKMLLVQSQAYRKQY
                     GFNSIYLLPVNLYGPRDNFNPKSSHVIPALIHKCYEAKLNHISEVVIWGDGSPTREFI
                     YAGDAAEGIVMAAEKYDGPEPVNIGSGMEISIKDLAELIARLIGYEGKLVYDTSKPNG
                     QPRRALDVSRAREYFGFTAKMSFEDGLKQTIDYYISNRDAILKEN"
     gene            complement(17483..18016)
                     /locus_tag="PK898_00920"
     CDS             complement(17483..18016)
                     /locus_tag="PK898_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOU18623.1"
                     /translation="MTYETAYDSKDLDILKLLAENPQLSQAEIGKQLGVAVGTVNWRL
                     KRLVSDGFLRTVPGEGRKLNYFVTESGTALSRQLYTEFLERSFSAYRTIKKQMQDLLD
                     RCEADHFTTIQVYGDGDAAEICRLIAVSRGLKIVSSGGEQDPIISVNGLQISINFPEK
                     KASAQPLDGRNKENQDD"
     gene            18213..19406
                     /locus_tag="PK898_00925"
     CDS             18213..19406
                     /locus_tag="PK898_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOU18624.1"
                     /translation="MYRLSNTQKEVVLQWQRFFHQNPEISFNEVKTSEKITEILNHYG
                     IENHRVGKSTGISAEITGELPSDSPKRIMLRADIDALPMEEQNDLPWKSQNPGAAHTC
                     GHDGHTAALLGAAVLLNDDRASFSGSVRLIFQPAEESVGGASIMIREGELDGVDLAAG
                     IHFWPALQSGKIACEYGAAMAGCEHFCIRFFGKGGHISVPHLANNPILSASETFIQLQ
                     TAAAQQFSPFEELLIGVGKIVGGTQYNIIPEEALLEGTIRAFQPETLQKVRQVVRNTA
                     EHIAAANNNTCDISFGNGIPPVMNNPKAVDLAIETASALIGKENVLTEIPKQFTGDDF
                     SFYAVEKPAAYIKIGSWDPSRPETNNPLHSPRFNLDEKALYLAAEYFANYACAFLRNP
                     AEESI"
     gene            19710..21302
                     /locus_tag="PK898_00930"
     CDS             19710..21302
                     /locus_tag="PK898_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU18625.1"
                     /translation="MNKKFFLVFCLMLTAILFSTVGAFAQSESNSLTVALSVEPVSLD
                     PAGGLYIPEQLLIQQIYDPLIYADPEQNLHPGLATSWEVNPEGTEFTLNLRNDVKFHD
                     GTPFNAQAVKVSLDRAAVGTTVAAAAPAILTDYIETQVVDDTQVVIKFSTPHATFLQD
                     LTRPWLMISSPAAIEQYGDDYGQHPVGTGPFIFKEWAAQDHITLVKNPDYNWGPEFFT
                     HTGPALLDEIVFRVLPEAAIRLTAFQTGEALLVQDPSYLEASQMANDGSTQVFTFTAP
                     GMTSHQMINVEKAPTDDINVRKAMIYAVDQETIVQTAFYGLQSAAHSVISPTTWAFNE
                     EANNLYRYDPKKAAELLEESGWVDTNGDGIREKNGVNLHIEYPALPAYEEAFMELLAY
                     YLNQAGFEVNITKLDDAGISEFGYAGKHNILNMGWISRDPSVLSYVYLSDNIEGGNQS
                     AYSRFRNARLDEILKTAPQTIDADQRKALYEEAQMIIMENAIALPIHTYGNVYLAAKN
                     VKGFRFDPEGYPYFYEISLAAE"
     gene            21306..22232
                     /locus_tag="PK898_00935"
     CDS             21306..22232
                     /locus_tag="PK898_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18626.1"
                     /translation="MGSYLGRRLLTSLSILIGVSILTFLMLQFVPGDPVRALIGRQTV
                     TPERLEEIKQEMGLNDPIPVQYFNYLKKVLSGDFGRSLKNNRPVLDAIMEQFPSTLKL
                     TVFALILSTFFGILLGAISAATYNSWVDRFLSFFCLGGISLPPFFLALLLILFFSVRN
                     NWLPAVASMNDWRSIILPAVTLGFGEACWLARIVRSSILDEMNKNYQNLARAKGASNW
                     TILWRHVLPNARIPIVTAISIQFVYLMAGSVVVESIFARQGIGRLAVTAIQNRDFPLV
                     QGTVTIIAVVYVISNTLTDIFYAMADPRVRLQ"
     gene            22229..23101
                     /locus_tag="PK898_00940"
     CDS             22229..23101
                     /locus_tag="PK898_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18627.1"
                     /translation="MNEIKFITQNIETRRPSTIKLLLHNFNFVVGGIILIILIINTIF
                     APLIATENVNQANPTQTFLPPSNEHLMGTDNIGRDIWSRVIYGGRVSLAVGLAAMIIG
                     MVFGTLIGIVAGFYGSWVDSLLSWVTEVLMAFPGILLALTVMAILGPGLSNVIVAVGV
                     GSIPQFMRMARSSVMKTRELDFIDAARTIGCTDAQILFRHILPNILRPLIVLATLGIG
                     GAILEGASLSYLGLGAQPMTPEWGSMLSNGRAYLGNAWWISVFPGIGIFLAILSINMI
                     GEGFNEVLDPSNRQ"
     gene            23129..24667
                     /locus_tag="PK898_00945"
     CDS             23129..24667
                     /locus_tag="PK898_00945"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU18628.1"
                     /translation="MASLIINGKIKRVEPDETIETAILIDKGRILQIADPDRINLKIY
                     DRTEIIDAKGAVILPGFIDAHNHFTMMGTQRSELDLSGIRNREDIIEKIRQENAKLDP
                     QMPIIGFNYEFDFIHQDHRLCAADLEAVAPDRMIQISDRGGHLSVTTPSTLKKAGILL
                     NSREYADCIFPCGGNFGGFTGEICGVANSLLSDYLRIQFRDDQTLKRAWKQASNIATE
                     HGVTSIHALVVEEEFEKLIQFQNQLPIKLKIYTETKNCKAVKTAGLKQVGGCGRVMVD
                     GDTGPYTAAFLEPYRNRPNTKGLLYYSDEELEDYIWNAHSAGLQVALHCIGDAASEQF
                     LNAIENAQQRNPRPIRHRIEHFEFGTSEQITRAKKMGVAISLQPTFNHYWPHTTYFSD
                     LGEERAMRSDPIRSVIDRGVPVGFGSDCPVTPCDPLLTIHSAVNHSLPHERISPNQAI
                     RCHTLGGAYLGNEETEIGSITVGKAADLVFLAADPAQVIPDEIKDIPVLKTIINGETV
                     YSAD"
     gene            complement(24708..25442)
                     /gene="rlmB"
                     /locus_tag="PK898_00950"
     CDS             complement(24708..25442)
                     /gene="rlmB"
                     /locus_tag="PK898_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOU18629.1"
                     /translation="MKEWITSRNAAYEILRAGSREVFRLEIAKGATVKGRLEEIIRLA
                     EKRKVLPIQVERSRLDSLDENHQGMALEVSGFHYSDLPDIRNRAAEKQEPLFVLLLDL
                     IQNPQNLGTLIRTAEAAGMHGIIIPPSRAAGVTPAVTVSSAGATEYIPIVQLNLSQAI
                     RWLHEQGVWVIGLDGGAESDLADPQKLGGNLAIAVGSEGEGLRPLTRKQCDQLVRLPM
                     KGRIESLNAASAGSIMIYLSYLIHRQ"
     gene            complement(25456..26862)
                     /gene="cysS"
                     /locus_tag="PK898_00955"
     CDS             complement(25456..26862)
                     /gene="cysS"
                     /locus_tag="PK898_00955"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU18630.1"
                     /translation="MSIQIYNTLTRKKEPFETLEPHKVKMYVCGPTVYNKAHIGHAMS
                     ALVFDIIRRYLEYRGYEVQFAMNFTDVDDKIINRANEMHVDPFRLAEGYIEDFKRNLA
                     DLNIKPATYNPRASQEIDQIIKMVQGLIDKGMAYPLDGDVYFRVRKDSQYGKLSGRQL
                     DDMLVGVRKEADDRKEDPLDFALWKSAKPGEPSWPSPWGPGRPGWHIECSAMNYHYFG
                     ESIDIHGGGNDLIFPHHENEIAQSESFTGKPFARYWIHNGMLQLRGEKMSKSIGNIIS
                     IDEFLEHHSADSFRYLILNSAYRNPIIFSEEVLLQAEKGLERLRSAFQPANPSAAGAP
                     QEVSEELRSQIEATREGFIASMDDDFNSAGALGQLFELVRMINIARDAGATDAELEPA
                     QAMLRELTDVFGLLLNEPKADRNQAAADPFIQLLIDLRTELRKQKNWELSDKVRDRLA
                     ELGVVLEDSKDGTRWHWA"
     gene            complement(26813..27928)
                     /locus_tag="PK898_00960"
     CDS             complement(26813..27928)
                     /locus_tag="PK898_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOU18631.1"
                     /translation="MNQSDKKNRTIGAVSFGLLGGWLGYYFSRSVTTSNLYPIQVLIV
                     SAIIGAVLGFFLVVPLIVHPVRRLRVKLENESSEKVAAATIGLIFGLVAAALFSIPAS
                     FLPDPLGEVLPFLIALLLGYLGVYAGISKINWFRKFMPPRFFEPEESDENEKITLVDS
                     SAIIDGRIAEIVRTGFLQGKLVVPNFVLQEIQFIADSEDILKRQRGRRGLEVLRQLQK
                     EFPAQVEIRDIAVENIRDVDGKLIALAKKINCPILTDDYNLNQVANLQGITILNINDL
                     SNAVKSVILPGETLMIHVLQEGKEYNQGVGYIEDGTMVVVENGRMYIGQEISVMVTKI
                     HQTAIGRMIFAKPETEMEQQPYEYTNIQYADEKKRTV"
     gene            complement(28326..29561)
                     /locus_tag="PK898_00965"
     CDS             complement(28326..29561)
                     /locus_tag="PK898_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU18632.1"
                     /translation="MHFPLKLKQNFQRIPLQIWVIFAGSLIFSIGSSMAWPFLNIYLR
                     ERLNLPLRTTTLLISLRAVTGILASFVAGTICDKFGRRGVMLASLLGGTVYYFLMVYA
                     QTIWQFAILMGIWGAMDLFYPIGSNSMIADMVKAEDRLEVYSLMRMVNNAGIAIGPIF
                     GGILAIKSYSLIFYSASIGYFISFVIFLFTVKETLQKRTITGNEVKIPTKGYHDVLKD
                     GVFVSTILMAVFIYMGSSIVFNLLSLYASETYGILESQISYVFTVNALMCVFLQLGAM
                     KFTVRIHPMLVMVISSLFYTVGLLLYVVCANVIWYCICMGILTIGELLMSPTMLALTA
                     KRAPADARGRYMSIYNLSHPIGNAFGPALAGLLYDQFIPQSIWYSAGFYCLIAAIGFS
                     VLYRKNKDASWLQKISIDE"
     gene            29738..31516
                     /gene="metG"
                     /locus_tag="PK898_00970"
     CDS             29738..31516
                     /gene="metG"
                     /locus_tag="PK898_00970"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOU18633.1"
                     /translation="MKQPVLVTAAWPYANAFIHVGNLSGAYLPADIFARYQRLKGRDT
                     LFVSGTDAHGTPITVRADDEKTTPEVIYKRYHESFLDMQQKVGISYDLFTSTHTENHF
                     KVSQDIFLALQENGYLYKATEKQWYAPSQEKFLPDRYVEGTCYICGYDNARGDQCDKC
                     GNLLDPEKLINPRSKIDGSTPELRETEHFYLDLGKLQNRVVDFLKSREDYWRPNVIRQ
                     SLGQILAEDLHGRAITRDLEWGIPVPVQDAVGKCLYVWFEAVIGYLSASIEWAKLQGT
                     PDIWKDWWEDDTCKSYYFIGKDNIPFHAVIWPAQLIGTGTKFGVYFEGKENKPFVLPF
                     DVPANEFMNLEGKKISGSRNWAIYVNDFLERYDPDPLRYYLTVNMPESQDTDWDWEDF
                     LKRNNNELVATWGNLANRVISFANKHWGQVPDPGTLEMEDQDLIDTIESGFLTVGDEY
                     EAVHLRAALAETMRLATEVNRYLDIAAPWQLIKTDKEAAGRKIYTALQAINNLKVLFS
                     PVLPFTSQKLHNMLGYSSNLFGEQVIKTVEDNLGEHRVNTYDASEAIGSWQPAELPVG
                     QKFGEVCPLFKKLDTSIVDEERARLG"
     gene            complement(31588..32763)
                     /locus_tag="PK898_00975"
     CDS             complement(31588..32763)
                     /locus_tag="PK898_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU18634.1"
                     /translation="MEEKNHRWSNPIRYMALVGLILFAGAVVYLSHETLNLIIAVAIV
                     AYILSPVVNFFVRKLHMGRGFAVVLAYLILIILIIILLVLIVPVITGRIQGFLSTDWP
                     TVINSIDSWLEGMIRQLEVQKIQIGNVGVDLSVPLIELRGKLNSINVEDFNISSIIPD
                     ISSAWQSIISVSANLISRILGGLLTIVTSIMISIHLCRDGWKLKDWIVKQFPENYQIE
                     TDEFLNRLGNIWNSYFVGELKLMLIIGIVTFGVCTALGLHWALPLGIVAGFLEVIPNI
                     GPIISCIPAIFSAAIFGSYWLPLDNFLMVLVVIIAYILIQQIENIIVVPRVMSHALDI
                     HPVLIIIGILILNKKIGLFGALLAAPILGLLKVTLHFVTCKIRNEDPYPELYNPPSA"
     gene            complement(32775..33974)
                     /locus_tag="PK898_00980"
     CDS             complement(32775..33974)
                     /locus_tag="PK898_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOU18635.1"
                     /translation="MESKDGSNGVVQKKMGQQNKRKKSISDLWLKIIQAGLGERLLKI
                     GSAVVTVLLIGTVLWVTGNFFMEDTQTDAINLQETLRSTPVRQSAVLPYYDPAYPKGE
                     LKPLNEGVFRSTKNDTELPAKARTDVIKYVVQKGDTVTSIAEKFNLRPSTILWANPYT
                     LADNPHLVVEGMELNILPVDGVYYDWHDGDGLNGVAEVYGVTPEDIINYPGNGLDPAT
                     IGDYSHPNIAPGTWLIVPGGSREFTNWSAPFITRENPAVASIYGPGACQSVMDGPVGS
                     GVFIWPTTETRISGYNYSEETNHRAIDIAGSTGNAVYASDAGVVVYAGWNDWGYGNVV
                     MIDHGNGWQTLYAHLDSYNVQCGYYVAAGDVIGAVGTTGNSSGPHLHFEMRYNGIPQN
                     PNNFFNK"
     gene            34128..35222
                     /locus_tag="PK898_00985"
     CDS             34128..35222
                     /locus_tag="PK898_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU18636.1"
                     /translation="MTANIYPFTAIVGQEQMVRALILNAIDPRIGGVLIRGERGTAKS
                     TAARGLAAVLPEEEVIAECRFGCDPDDELHWCTECKERKAKGIPMTRTRRKTPFINLP
                     VSATEDRVVGTLDIEKALKTGEKSFEPGILASANRGILYIDEVNLLEDHVVDILLDSA
                     AMGVNSIEREGISFSHPARFILVGTMNPEEGDLRPQLLDRFALSVDIRGIEDARERVE
                     IMRRTLRFEDSPELFQAEWQKSEAELTEKIASARKRLASVRYTSRDLITIASLTSSLK
                     VEGHRADLVILKAARAQAAFENHPSVTDLDIAMAAELALPHRLQKGPFSESTVGTDEL
                     KQRIDELHGKTSDSTSFQSESDAGDEEKKI"
     gene            35258..36160
                     /locus_tag="PK898_00990"
     CDS             35258..36160
                     /locus_tag="PK898_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU18637.1"
                     /translation="MAQYSQGGDSIFNNAEANWWDNAQNLFFINPELLDDNKPNLSQK
                     KSSGKRSHIISKDRRGNYYKARIAEDAVNDLALDATLRAAAPYQKSRRTNPSDRSILI
                     KHQDLRRKVRSQRASGLLLFLLDLSWSMAVRQRMNATRGAILSLLTDAYQHRDRVGLI
                     TFQKDSAELVLPPTYSVTLAERSMKKVQVGGKTPLTAGLWKAYEVLERERKRHPDENA
                     LLIVLTDGVGNISMFDKAPEEESKIIAEKIAAENFRSIVIDLDTQDFDQGMTRKLAAQ
                     LKAPCYLISELKADRLAKIIRKEI"
     gene            36360..37436
                     /locus_tag="PK898_00995"
     CDS             36360..37436
                     /locus_tag="PK898_00995"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18638.1"
                     /translation="MDEKTSLKEFLKSIISLPGISGYETTVSSAIAEKWRPLTDEIIV
                     SPVGNLYGIKKGFLSKSGKPSKRIMMAVHMDGIGMVIERIEKDVLYFAPIGGFDPRIL
                     PGQEVIIHSRSGDIRGIIVQLAPHLLSEPFTGKPVPLNQLVIDTGLNAEELKETVQAG
                     DTVSYANLPFEMPSDCIAGHSLDNRASVAAVTQCLTELQRFNHAWDVYATGTTQEETV
                     FLSRTTVSDVKPDIAIAIDVTFAKGPGVDNYKGKPLESGPSIGFGANIHPVLFNQLKQ
                     LCEQLDIPYTLDPLPQMSGTDAHPIQTASGGVATCLLSIPLRYMHTPVEMISIKDVMR
                     AGHLMAEYIARLDENSLDSIKWED"
     gene            37441..38529
                     /locus_tag="PK898_01000"
     CDS             37441..38529
                     /locus_tag="PK898_01000"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18639.1"
                     /translation="MIKEEKSTIDSLPVLPESSVRLLEELSNAVGVSGNENEIRSIIR
                     REVSSVADNLTTDTIGNLIAVKHAKNDHALRVMVAAHMDEVGFMIVDKESDGIFKFQV
                     VGGIDERALAGKAVQVGKKHAPGVIGACPVHLSTPAEREVILKTDNLRIDTGPGSKDI
                     EVGDYATFATKFKRMGGSFCGKAIDNRIGVATLIELLKNAPDSIELIAAFTVQEEVGL
                     RGAEVATYNINPDFAFIIDCTPANDQPAWDHSENTLYRTKLDQGPAIYTNDSRTLYDH
                     RLIRYLSDLGDRYGIRYQYRQPASGGTDAGAIHTSRSGIPTISLSVPGRYTHTAGSII
                     RKADWEDHYRLMTVALRHIDADILKENR"
     gene            38563..39555
                     /locus_tag="PK898_01005"
     CDS             38563..39555
                     /locus_tag="PK898_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOU18640.1"
                     /translation="MKNLLEKLTQTTGVSGYENEIREVIRKEIEPFADDIQIDVMGNL
                     IARKGIKKSGGKRIMISSHMDEIGLMASHINDKGFVYVATIGGVNPLTCFSARVKFLN
                     GTEGVVYAETQKMDKVPTVDQLFVDVGADSAETCPVKVGDEAVFVRSFLDMGNRVVSK
                     AIDDRIGCLVAIETIKALKDSPNELFFVFSTQEEVGLRGAKTSAFSINPEMGIAVDVT
                     LAADVPHCEPGNPVLGKGPCIKVRDGSLIAHPKVIDAMKKAADRTGIEVQMEVLSHAG
                     TDAGAIQLVRSGVPAGCLSIACRYVHSPSEMVDINDVSESVRLLVDLLSNPVDL"
     gene            39596..41002
                     /locus_tag="PK898_01010"
     CDS             39596..41002
                     /locus_tag="PK898_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU18641.1"
                     /translation="MARIHFFKKHPIRFGAAVLVSALLVILIISGCSEVKKMLAGTPT
                     ATIMPAVKQTATPVSNEEEIILPENESTSESSPTVDDSDETSVPNRSNSENRTLTLWV
                     PPQFDPSSNDEAGKILTEMLKIFMEENPNTLINIRVKSLTGEGSAINTLSAAVNAAPE
                     VIPSLIIMNRNDLVSAVQKGLIYPISTGLFTDPASWYPFAKESSTVNNLIYGIPIAGD
                     PLVLGYRPSKTGSEINTWDEILSRGLPIAFEPSNPYDLFGTFIYLAKGGKITNDMGQP
                     WLDQAKLTDTLNFFLSGGQKGAFPPSLAQGTSGSGANENWQLFLEGSLHIIVTKLTTF
                     RHSQNADIDAIPLPLFNGSSSYPLVNTWNVVLSNSNPEIQNLAVKLAEKFADPRFNDQ
                     WTQSAGYLPVRSIGQTLWQNDSFYEKMITICENGELIQTDQILNKITPVINEAVSSVI
                     KTGATPEDAAEKAIDAIN"
     gene            41019..41369
                     /locus_tag="PK898_01015"
     CDS             41019..41369
                     /locus_tag="PK898_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18642.1"
                     /translation="MSKNSKSQRRRFNWILFLIICILVVFAFILSESDKIEHASQTSR
                     TIQMGLLQTAEPTIILPTPTPFPSDYPKLPAEYFENEDQTDGVILGGTVLVLIILIGT
                     MVFIQKEHQKKNEK"
     gene            41359..42717
                     /locus_tag="PK898_01020"
     CDS             41359..42717
                     /locus_tag="PK898_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18643.1"
                     /translation="MRNKNRPLFYENKKKTWPYVVFILITIVILIIWGAQVPVIRQTL
                     GRSVLDGISYGKSLILPVSTVNPLNYRIQTYYPTLDLPSKEVIFAENLEDINSSEPYL
                     EITFTPTPTPGVLWVIEDPEKSSTNDSATLSEEVNDRLEMPIFEMADYLNDGPASLST
                     FLRFYEKPESQYLVSEKIKPDYFDPNVSLNEMLSYSEQKYPDLSGIIRKNGNIQILAS
                     LIDSHFPVLIRLQYQRQQSAWKGDDLWDARYVVISGIDSKNKIVYFSDPLKGNEQTLS
                     FDRLMEDWYPFRREYLVIYPVDREESLALLLNSDWDEEENSKKALEKFQTDVTMVPDN
                     QFAWFNAAALLIENNHNEEAWDSFRTALQVGIPQRYLLYDFSLYEAAFKNGLAEELRD
                     RTAAMLKINNHSEENWLWNGWAYTLLQDRSEAEKCFQKVLDINPNNSDGQYAMEYLKQ
                     YE"
     gene            42738..44147
                     /locus_tag="PK898_01025"
     CDS             42738..44147
                     /locus_tag="PK898_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18644.1"
                     /translation="MEKKNNNKTIIIVVITVIVLIGCLCLSAAALGVYFLNHRIDKNN
                     EELIYETAVPETSPEDQTALPEVTPDNETDGTEINNTEITQKQQEIIKRAEKIRGLKA
                     QGDFNITFLTKEELRDQLINDMYQDTTKQDFQDEHDILTLLGFIPAEMDLESFYLDFY
                     TEQIAGYYDEDANVMYLIKGGSETDNSLTLAHEYTHFLQFDHFNPAKTLNYNDEYCEK
                     NQEYCFVLGSVFEGDATLTEMLLAAQPDLGLYDERMVEQDTSVFDNAPQYFQNYLLFP
                     YTYGFDFVSSVYRKGGFSAVNDLYSNPPLSAEQIMHPEKYSADQPVNVLFEPFQKNLD
                     ESCDLVYDNVLNEADLLWLLNSGYEKSWRISDKSAKKAAAGWGGGAFQFARCDGKPMF
                     FGKTVWDTVNDAKEFSQSLKDYNDKRWGKSKINGSWTGTDGEQIYVSVQDDKVFYMIA
                     PAEFSPDSLIDLIETGQGL"
     gene            complement(44229..45548)
                     /locus_tag="PK898_01030"
     CDS             complement(44229..45548)
                     /locus_tag="PK898_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU18645.1"
                     /translation="MDLFDYTDRENMMQTTPLAARMRPRSLDEFVGQEHILGEGKLLR
                     RAIESDKLFSSIILFGPPGTGKTTLAQIIAAQSKAYFESVSAVLAGVADLRKVIVDAQ
                     ERRKLYRTRTLLFVDEVHRWNKAQQDALLPHVENGLITLIGATTENPYFEVIKALLSR
                     SRVFELKPLNETDLNIILNRALSDPERGYGKLNVKLDDDARRLLLDLAGGDARNLLNG
                     LELAVESTKPDENGIIHITLKIAEESIQKRALQYDKNGDMHYDTISAFIKSVRGSDPD
                     AALYWAAKMLLAGEDPHFILRRLIVLASEDIGLADPNALQVAVAAATAFDYIGMPEGY
                     YPIGEAILYLATAPKSNSVGAIFKAMDHINEQGTGQVPLPLMDASRDAKGLGHGQGYL
                     YPHEFPNHYVKQQYLPDGVKTDFYHPGEQGYEKKIKEWMEYLKKNSE"
     gene            45715..47286
                     /locus_tag="PK898_01035"
     CDS             45715..47286
                     /locus_tag="PK898_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOU18646.1"
                     /translation="MNKKILSVFLVLSVLLTVVFSAGAQDTAAETVKLTILGTNDIHS
                     YTADTPDNVAEGETAKTGSIGYAKIAAYKKQVAQNGPVLLFDSGDATHGQVIATLLEG
                     ESIIWLMNEAGYDAMTPGNHDFNYGYQKLIDFNYMADFPILSGNLVYADSQMSVLPEY
                     TIFDVDGIKVGVFGISTPETLYKSSPVNTEGLQFIDPYEAGKYYADFLRPHVDLVVCL
                     SHLGLDAGSEFTSEGLANAVPGIDVIIDAHSHTELPEGKLVGNTLIAQAGEHGRFIDQ
                     VEIELAAGQASTDRMTVVSKSAKLITYGDLLNLEPDPDVLEINTEIEKQINEISSVVI
                     GKTTVDLDGERETNRKQETNLGDLIAQAMLLETGADVAITNGGGIRASIPAGDVTLGQ
                     VITTFPFGNYVVTIEATGQDLIDAMENGLSDYPELKGAFPQIAGMRVVFDPSKPAGSR
                     VVELTVDGKPVDPAATYVVATNNFMAIGGDEYTMFADDKLLGEYSAFDEILADYLTTY
                     GTEGLEVDGRISTVQ"
     gene            47596..48330
                     /locus_tag="PK898_01040"
     CDS             47596..48330
                     /locus_tag="PK898_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU18647.1"
                     /translation="MLRIAICDDMADELQILLSLTNQYISTNHLDVDVKEFTHPDQLL
                     TAIIIESFHLYILDVVMPMISGLELGKEIRRLDQEAQIIFVTTEPQFALQAYAANPIN
                     YLIKPIQKQQFFDTLALVVSKADFADEQTFAVKTAEGLRVIRLRDIICCEYRCHAVIF
                     SLTNGEEILSRTFRENFSNYASTILKDRHFLQCHSSFVINLRRVERFTKENFTLTGGK
                     MIPIAAKQYPTVRDTYMDYLTGIGNR"
     gene            48330..49616
                     /locus_tag="PK898_01045"
     CDS             48330..49616
                     /locus_tag="PK898_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOU18648.1"
                     /translation="MKELFPDLVRAAISTTANVLLMMTLLQPRYSKKITLLTMFGILA
                     ADLGTAIYCYLSRNLTLLSKLDVILFAVLCFAVRPLFKDTFMQWLFSYLTVQNISDVV
                     IILSFIGSRHLPYPVYANSLLRFILFGVFLLILSRRVRPLYRQAVEHWTAYFAVALSI
                     YLTFNYYVLSSDDIVVTLTEQAIPLLLVIFISLAAYGSIFLSLKNLQQEFQIKEENRK
                     MQAERDYLQLAARNMSRRLELMAEVSEQNSRAAHDRHHFNNVLLELLEEGKTDQAQAL
                     LQSQNQVAHQISKVYCENSAVNAAVCHYTDLAEQEGIKTEIMLDIPSNLSVDALEFSM
                     VVSNLMENAVQACKRLPEEQVPYLRFTCRYVGRLLLEMENPCANNSMLDECGYPVTPE
                     ENHGIGSKSVIAFAKKYDGELIYKIENSIFQVRLLI"
     gene            49776..51098
                     /locus_tag="PK898_01050"
     CDS             49776..51098
                     /locus_tag="PK898_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU18649.1"
                     /translation="MKKRILASLLMSFLLASLALFTTEFSQNIGVSGAAAPEDAPALW
                     NAGDTRNKIVVISDIHLGIDDNYTETLQNRPLLVDFLRRLQSTNDVRELVIAGDFLDE
                     WFLPVYYPSYTDEDRFYQDVIVNNRDVIDELNNVIDSGIKLVYVPGNHDMTLNAGILL
                     EAIPDIIQARDSKGLGTYYTGDRNEIAIEHGHRYDVFSAPDTVTNRELCGNDDTIFPA
                     GYFYARYAATWVLEGRPAVKKDLPVVTDIPDKTDSDQYGAFLYYSILKDISARMTPKE
                     SPEEKIFDIHIAGFDDAYSYLDFYPAQQSDGTISAPVLFKNIQRTWDERQTLNNVKVP
                     NSFIEAVSGTIDWEYFFRQAKAQYLENPDESVDVVIFGHTHVPVYKDLGNGKYYLNDG
                     TWIDHNTDFPDATRTFAVITTGNKDQVALYKFEEDGSVTDISASVSEQ"
     gene            51258..51473
                     /locus_tag="PK898_01055"
     CDS             51258..51473
                     /locus_tag="PK898_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18650.1"
                     /translation="MEKNKHENSLLNSPNSEGNVWPKQVCPAFEPRSDTPKELRQCWY
                     CQFADFHLNKPRSLDVGVCYWPNKVLS"
     gene            complement(51657..52826)
                     /locus_tag="PK898_01060"
     CDS             complement(51657..52826)
                     /locus_tag="PK898_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU18651.1"
                     /translation="MNFSFYDPVRILFGRGQLNNLHLQNFPGKKALIVISAGKSIRQN
                     GYLGRVEKQLDMAGIEHVIFDKILPNPILSHVTEGAKVARDNRCDFVIGLGGGSSIDS
                     AKAIAVMATNDGNFWDYVGAGSGKGKPVEKKPLPIIAITTTAGTGTEADPWMVITKED
                     TDEKIGSGWDSTFPYFSIVDPELMLTVPPALTAYQGFDALFHSTEGYINVTANVISDM
                     FAMKSIELIGANLAEAVANGNDIDARENVALANTLSGIVESLSGCTSEHSLEHALSAF
                     HHNLPHGAGLIMISKAYYTFFAKSGACDEKLIAMAKGLGKKDAKEPMDFVEALVDLQK
                     KCGVDALKMSDYGITKDELPEVVKNARHTMGGLFNVDPAVLSDEDCLKIYEESYR"
     gene            complement(53023..54483)
                     /locus_tag="PK898_01065"
     CDS             complement(53023..54483)
                     /locus_tag="PK898_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOU18652.1"
                     /translation="MNTIFILIDSLRRDYLGCYGNTWVQTPNLDALAENSTVFESSYI
                     GSYPCMPARRDIWTGCLEFPWRGWGPLEPEDVDIAKILTRNGHTGMVISDHYHMWERG
                     SGNYFFNFSGSEYIRGQENDLWITDPTIPVEYSADPERMARHSLRSESYARYKRNTAH
                     FRLEEDYFAPQVFRKASEWLERNRTLKDFFLMLEVFDPHEPFDPPFPYNEMYNPGYNG
                     QSFIWPTYGKSDLYSEEELKQIRSLYAGEVTMVDRWLGYFIDTVKHLGLMDDTMIIVT
                     TDHGHLFGEHGMIGKPWSDLGDSNMYQEIASIPLIIFNPKEQSGKRVSNLTQPVDLFA
                     TVLDAFELPIPDGIHGLSLLPFTLKTEKDLPKREIAVYGRYGESINITDGEWTLFLWP
                     PTKSNEPLYWYSHLPPQFGDVKVRDDFDGKRYSAIVTRGPMSNALYHIKDDPCQEHDL
                     YHQRPDKVNELKEKLKSYLLSIHAPDEQLIRLGLVE"
     gene            complement(54512..55714)
                     /locus_tag="PK898_01070"
     CDS             complement(54512..55714)
                     /locus_tag="PK898_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOU18653.1"
                     /translation="MTTASDSRPPAFHVMAKPRGAICNLGCEYCYYLPKKALYPGGTF
                     RMSNQVLKSYVRQYIAAQQVPQVHFAWQGGEPTLMGLDFFRRVVGYQKEAARPGMRIE
                     NAIQTNGTLLNDDWCRFFAENHFLVGISLDGPREIHDVYRKDKGNHGTFDRVIHGIRL
                     LKDYQVDFNILTCVSAANAEKPLEVYHFLRDEVGAEFIQFIPIVEWERPTDPQKERKL
                     SERSVNGEQYGYFLNSIFDDWLAHDLGKIFVQIFDTSFGHWIGAPGGLCVFQETCGLA
                     LVTEHNGDVYSCDHFVEPDCYLGNMLKTPLAEMVGSEKQRQFGLAKRNSLPQYCRSCS
                     FLFACNGGCPKDRTDITPDGEPGLNHLCLGFKTFFAHVDSPFRIMANLIRQHRPMTDV
                     MKILENSN"
     gene            complement(55783..56616)
                     /locus_tag="PK898_01075"
     CDS             complement(55783..56616)
                     /locus_tag="PK898_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU18654.1"
                     /translation="MKKKTFGALFRHGFLLAVVIYTLIPIFFTLGTSLKLFRDIISGS
                     LLFKPTLINYERLLNPSQSNFLRLTTNSLLIAAGTMAVVLVIASLAAYSLSRFNWHPL
                     WNGLIQGIILFIHMMPPVTFLSPLYLISRALNIYDTPVAVIMGHIILTLPTAIWILLD
                     FFAEIPKEIEEASIVDGASHFQSLTQVMLPIIQPGLASAGALTFIFSWRDFLFALSLT
                     TTPRGMTIPVGISSFVQEYSIRYGEMAAGSFFAMIPALLMVAFAQRYIIKGMTMGALK
                     E"
     gene            complement(56637..57566)
                     /locus_tag="PK898_01080"
     CDS             complement(56637..57566)
                     /locus_tag="PK898_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU18655.1"
                     /translation="MDHSTYFSLSSKPPGWKTRFSNFIDRTFKIWTILPLILVLIFLV
                     AYPTVQLLRMSVSEVKIVQGKTVWTFVGLKHLYTALEDQIVPVALRNTIVFVIAVVIL
                     ETILALVIALLVSRSHHLVGLYRTVFLIPLLIPPVAIGTMWRLMYDYNYGFINQVLGL
                     IGIAGPTWTADKKLALPSIILVDLWHWTSFMFLIILAGLESLPQEINEAARVDGASEA
                     QVFRFITLPLLKPTLVTAVMLRTIFAFKVFDEIYLLTGGGPGTATEMISLYIYKVFSN
                     QFRMGYASFLALALAIIISVFVIFYRRIGRRLA"
     gene            complement(57727..59070)
                     /locus_tag="PK898_01085"
     CDS             complement(57727..59070)
                     /locus_tag="PK898_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU18656.1"
                     /translation="MKQTIKKPVLIWGISLILILSFVLSGFNPVPSTAKAALPPITIQ
                     INESPWYPGFEALVNKYIKDTGNQVTLNRIPFNGMLEKSRNAVQASESEFDILTLNEQ
                     WYMQFYADGLVTPIKEIDPNFELDPQIIEYEWATRWDNKLKYSTKDGEIYGLPINGNI
                     MLFFYRKDLFDANGLATPQTWDDVLAAAQKLNKPSKFAGYVVRANPPNWEFQAFLASY
                     GTSIIKLDEQTGEWEVTINSPQALEALKMWLKLGVEYGPANYADNGQAEMDALMASDK
                     AAQMVLTCAVATDFDNPEKSIVQGKVAAVPVPGATAGSNATMSGIWVMSIPHNLPKER
                     KEAALAFLKWAMTKDAQLYYAQAGAIPVRQDVYEEMSQDPKVGWWMKAMADSTPFIHA
                     QPRLVETPQIIEVIDRRLKQALISETTPEETLSEAAKEIFKILKDAGYKVKPLAE"
     gene            complement(59158..60189)
                     /locus_tag="PK898_01090"
     CDS             complement(59158..60189)
                     /locus_tag="PK898_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU18657.1"
                     /translation="MQDSKQMVTLKNIADIAGVSIKTVSRVVNNDGEVSEDTRRRIQR
                     IIDDLGYYPNTIARNLVNGRSNTVGLVIQHSANYIFSHPFFNEVMRGIAETLSEHNIN
                     LLIHLANKDMPYSHLYYQRLVDGLIFMSIPVNDPNLPELSENNFPSIFTCRIAEENNP
                     TCWVDSDFIDGMEQAIDHLYSLNHRRMALLAGPENLISVRLRVKGYKKALTEKGIPVD
                     ESLIYYMDFSSDAGRNLALQLMKSPNPPTAILCGDDMIAIGAVQGLSEAGWQVPQDVS
                     VIGFDDIELARFSTPPLTSVRQDAFLKGQIAAKTLYRMITKEIDEKPFQQILPTRLII
                     RGSTAVSPR"
     gene            60429..60878
                     /locus_tag="PK898_01095"
     CDS             60429..60878
                     /locus_tag="PK898_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18658.1"
                     /translation="MNPFSPSAKILEVHETEQKGFQPLVTFQTWRVAMMTDLQSESPE
                     KFYRLQRHMKTDEVFILLQGKCILFLADGEDPIEHQFAEDLKLFRVYNVKQGIWHSHI
                     LSEDAKVIIVENADTSPSNSQYLQLSDSQRERFSFMAKELWGTLPSE"
     gene            61041..61823
                     /locus_tag="PK898_01100"
     CDS             61041..61823
                     /locus_tag="PK898_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU18659.1"
                     /translation="MTLLFFFLSLAASTIGAISGIGGGIIIKPVLDATHTLSISTISF
                     LSSCTVLSMSCVSILRNRKQSVSINYKMSTLLGIGAIIGGILGKELFEMAVSHFPSEN
                     MVGAFQAFALLIITITVYFYIRRQKNIHTKRISNPLLVILIGTLLGMISAFLGIGGGP
                     LNIAVLYYFFSMSGKTAAINSLYIIFISQVSGLFNLVVSKRIPPFDWSILLLMMIGGI
                     MGALIGYRINRKLSDAHVEKFFCCVLILIGILNIWNFYRFAF"
     gene            complement(61922..62944)
                     /locus_tag="PK898_01105"
     CDS             complement(61922..62944)
                     /locus_tag="PK898_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU18660.1"
                     /translation="MATMRDIAKAAGVSPATVSRVLNHDPTISVTTATKLRILNVAEE
                     LEYVKKNGRKASAKERLNIAIVDWYSEADLAEDPYYLYLMTAVEKYCASQNINTFRVV
                     NVNGVYLNSVDLKVDGMIAIGRFLISEVEQLSKITSNIIFLDSSPDEERFDSIMVNTK
                     LGTKQALDYLFSLGHRKIAFIGGEVVGNNREKTLDSRKDMFVSYMRDHQIDVSDLVFE
                     GSRLSFTEGSRMTQELLNRHKDLPTAIFAANDNVATGVLTTLSMNGIKVPSQISLVGF
                     NNLASVKFLNPPLTTVNIPIDYISEVAVSTLQKNIDRTVQNTLSMYVSTQLKIRKSCA
                     EPRTNS"
     gene            63195..64463
                     /locus_tag="PK898_01110"
     CDS             63195..64463
                     /locus_tag="PK898_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU18661.1"
                     /translation="MKKTFVCLLVIALFATVFGAVSAENSDGKITVWAWDPSFNIAIM
                     KEAAARYEAINPDVKIDIVEMAKADVEQKLNTILAAKTTEGLPEIVLIEDYNAQKYLQ
                     YYPGSFADLTTKFNYDDFAAYKVGVMTLDGKVYGVPFDSGVVGFFYRSDILAEAGFKA
                     EDLKNITWDRFIEIGKTVKEKTGKYMLGFQNTDGGIMRIMMQSAGKWYFDEEGKPALI
                     GNQALVEAFELYKKIVDSGIAKPTNNWNEWVGTINTGESASVTSGVWIVGSIKAAKDQ
                     SGLWDVAPVPRLSTVDSKNASNLGGSSWYILDKSADKDAAIDFMQKIYAGDKDFYQTI
                     LINNGAVGTYLPAATGEAYSTPDEFFGGRAIYEDLSAWMKEIPPVDYGIYTYEADAAI
                     MAQMEAVCAGTMTIDDAIKAAEAQLENQIQ"
     gene            64534..65463
                     /locus_tag="PK898_01115"
     CDS             64534..65463
                     /locus_tag="PK898_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU18662.1"
                     /translation="MGKKLSMKGWLFISPALILLAVFLVYPIIYSFYLSFTSTKGIIS
                     SFVGFKNYIRLTKDPMFLLALKNTGIILVIQVPIMLISALILANILNDRKLAGRGIFR
                     TAIFLPSVTSLIAYTILFKMLFSFDGIINRLLLSTGIVDHPLEWLTTPKLAVFVLILA
                     MLWRWTGYNTVFYLSAMQNISYEIYEAAEIDGASKTQTFLRITLPLLKPMILFTTIMS
                     TIGTLQLFDEPMNLSQGGVTAATMGPGNCFLTLSVYIYNLCFKYVPNFGYAATVSYVI
                     VFIIAALTVLQFWVGQSNEEKDAKKRLEKEGKA"
     gene            65460..66278
                     /locus_tag="PK898_01120"
     CDS             65460..66278
                     /locus_tag="PK898_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU18663.1"
                     /translation="MKRKFRVSRIFLYLFLIIVSFICVFPFYWMIVGSTNTPIQIIAG
                     KMTFGSHFVENFHTLFNNYNMLRILMNSLKISGISVILILLIGGMAGYGFQMYKSNLK
                     EKLYGFFLLTMMVPFATLMIPLFKIVTKLNLLDTHFAVIIVSAVSAFMIFFFRQSFVN
                     YPTEILEAARVDGAGEFRIFFNIFVPSMKSTFAAAAIYSFITSWNSYLWPLIVLQSND
                     KKTTTLLISMMSSSYTPEYGVIMLAIVVATLPTIIIFFAFQKQFVQGMLGSVKQ"
     gene            66294..>68230
                     /locus_tag="PK898_01125"
     CDS             66294..>68230
                     /locus_tag="PK898_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU18664.1"
                     /translation="MNRINMHNLAWLEDPEIFAVNRIAAHSDHDRIDEAGNIIPPFSL
                     NGKWDFLYYEKPDDVDFDVIFDSNHPADFKSVIVPGHIQLQGFGKPQYVNTQYPWDGY
                     EKPIPPQIPKYQNPVGFYIRSFDLPDHFDPENTRISFQGVESCFYVWLNGGFIGYSED
                     SFTPAEFDLSPFLKKKDNRLCVMVVRFCSGSWMEDQDFWRFSGIFRDVFLYQTAPVHI
                     QDIEIKTHLNDDFSLAQVEANILLLNKNPRDITVSFSLADGKLTKSIQAEALYDEENC
                     SFSMEIKNPKLWNAEEPHLYRADISIREAKSGDFISAARTEIGFRRFEIKNKIMMLNG
                     KRIIFKGINRQEFYSKKGRAIDAGDIEEDLKILKRNNFNAIRTSHYPNNSAFYRLCDR
                     YGFYVIDEANLETHGTWMVLGKVNRTEFIVPDDKPVWEAAVIDRARSMLERDKNHACI
                     LMWSCGNESYGGPVIKAMSDWFHQRDSSRLVHYEGISFDRRYDDTSDVESRMYAKPAE
                     IENYLINNPQKPFILCEYAHSMGNSFGNVQKYIDLTEKYPLFQGGFIWDFMDQALLTK
                     DERGNEYFAVGGNFDDQPNDGYFCGDGLLFADRSPSPKLAEAKFLYQPVSITCLPESI
                     RITNHQLFTDTSGYRFEWVLTG"
     assembly_gap    68231..68330
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <68331..69167
                     /locus_tag="PK898_01130"
     CDS             <68331..69167
                     /locus_tag="PK898_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HOU18665.1"
                     /translation="HIIDGDYNVGIKMENSFALISKANGKLVSIQSEGTELLRSPISP
                     DFWRAPTDNDLGNGNTFRWAQWKIASLYQKCERVSVDPERAQVTSFFFMPANPKIHCS
                     MEYSFFKKNIVRIRMHLDPVKGDAPCVGIMFKMPGKWNRLSWYGNSQSEAYSDRQSAC
                     RIELCEGSVDRQYVPYLHPQECGNKTQLRFFTVTEDRGKGLRLSSDSPFEASALPYTA
                     HEIENASNILHLPPRNETVVGAYKQKCGVGGDDSWGAPVHEEYLIKTDEGIDFTIYLQ
                     IL"
     gene            complement(69312..69938)
                     /locus_tag="PK898_01135"
     CDS             complement(69312..69938)
                     /locus_tag="PK898_01135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOU18666.1"
                     /translation="MLLEDDAIVLFQGDSVTDAGSDYTDETDLGTGYPMMAASYFSAT
                     YPEKGVKFINKGISGNRVRDLKERWTTDCIDRKPTWVSILIGINDCWRRYDSDDPTPK
                     ENFEADYRYILRQTRDKLHAKLILCEPFVLPVSEDREAWREDLDPKISVVRKLAAEFN
                     AIFLPLDGIFAQAAAKREANFWLPDGVHPSLAGHALIAKNWLQAVEAL"
     gene            70290..70511
                     /locus_tag="PK898_01140"
     CDS             70290..70511
                     /locus_tag="PK898_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOU18667.1"
                     /translation="MSAPKGKYAWTVKVGEKGQIVIPKEAREIFNISPGDTLILLGDK
                     KRGIAIPPKDVILKLAEVFGDSLPDQDTE"
     gene            70511..71236
                     /locus_tag="PK898_01145"
     CDS             70511..71236
                     /locus_tag="PK898_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18668.1"
                     /translation="MIAIQTVGLTKKYGKITAVDNLNISVNKGELFALLGLNGAGKTT
                     TIKMLSCLTRPTGGDAILLGDSIISKPLSIKQKTNISPQETAIAPNLTIRENLELIAG
                     IYGQDKKTAHQNSICMANRFELDNLMTKKVKALSGGLQRRLSIAMALISEPQILFLDE
                     PTLGLDVLARRELWSTIKSLKGKVTIILTTHYMEEVEELSDRIGIMSSGKLLATGTVS
                     ELMEQTNTNKFEDAFVILAKGVR"
     gene            71233..71982
                     /locus_tag="PK898_01150"
     CDS             71233..71982
                     /locus_tag="PK898_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18669.1"
                     /translation="MRAYIFSKRNGKEILRDPINLFFGLGFPLILMFLFSIINTAIPP
                     EANNTMFETKRIAPGIAMFGTVFLALFSGTLLAKDRTSSFLMRLFSSPMRSFDFIMGY
                     TLPMILFAVVQSSITLIAASFIGLPLTINTFLAVFVLVPISILFVGVGLLCGSIMNDK
                     AVGGICGALLTNIAGWFSGTWIPIDLIGGGFRTVSHFLPFFHAAETARLAIEGKYVDI
                     LPHLSIVLAYSLIVYGIAIFVFRYKMSSDRA"
BASE COUNT        20078 a        16935 c        15589 g        19469 t
ORIGIN      
        1 gacgtcgagg acgccgtccc ctacaaattc ggtgtgcttc gcaaggacgc cgtcctcgcc
       61 gttctgtaat tatgaaaatt tattgtaggg gtcggtgcca caccgaccct tgtttacaaa
      121 atacccgaac gacaattgta ggggaggcgt cctcgacatt ccgcatgtaa tttcatttca
      181 ctcaccctaa tcctctccca aagagctagg gaagattttt gtagaggatg gcgtgacgcc
      241 tgatcgcctt caaaatatca ggatatcagg aatggcgctt caggaattcc ataaaaggat
      301 ttgccaccgc atcgatttct tgaagctctt ctgcagtaaa cgaaagattt tccatcaccc
      361 gcagattttc agcaatctgt tccacattac tggcaccgaa cagtgcactt gttacccgat
      421 tatccttcaa gacccacgcc aatgccatct gtgaaagtgt ctgtcctctc tcaatcgcga
      481 tgctgttcaa ttgacgaata taaccgatac tctcatccgt aattagatct tttttcagat
      541 gccgatccaa cgaggcgcgt gatccttccg gaatgccgtt taaatatctg gaactcaaca
      601 aaccgtgctg tagcggcgca aaaacaattg tgcccatccc gtattcggcg gttatatcaa
      661 acagatgatc cgtttccgtc cagcgattca agatcgaata agaaggttgg tgaatcagac
      721 aaggtgtccc caattctctc aggatttccg cagctttttt tgtcagatcc gcgggataat
      781 tggaaattcc gacgtaaaga gcttttccct gacgtaccag aaaatccaat gcgcccattg
      841 tctcttccaa tggcgtttcg ggatcgggcc ggtgatgata aaacacatcc acataatcca
      901 gccccatcct tttaagactt tggttcaggc tggcaaccag atattttttt gacccataat
      961 ctccgtaagg tccgggccac atatcatagc cggctttggt cgaaataaat aattcatccc
     1021 gataaggttt cagatcctgt tcgtagattt gcccgaatac cctttcggcg gcgccgtaag
     1081 gcggcccgta attgttcgca atatcaaaat gggtaatccc atgatcaaaa gcataacgga
     1141 tcatagcccg atcggtctca aaacttttat acccgtcaaa attatgccag agtcccaacg
     1201 tcaaaatagg cagttgaacg ccgctttttc cgcagcgccg ataaatcata ttgtcatagc
     1261 gagcgggatc tgctgcatag acgcttgtca aactcggatg atgtgcatca atcattttgt
     1321 ttcctttcca gttcgttcaa aaacacccca ggcatcgttc atgccttcgt tgattgtttc
     1381 tcgataacga taatgatcgc aaagaaattg ataggttggt cgctgataat ccgccaaatc
     1441 aagatcttca cagaatccgt ttgaattata agggacttgt gccaaaaaac ggtcattcat
     1501 ataaacgata aatttaggcg ggctgttttt cagatcagcc aagaatgttc cccattgttc
     1561 ctcttgcata taaccgggga acagataaag cggccgaaca ttgaaaaagc gggacggaga
     1621 ggatcgttca ctcatcacat aaggcgccaa cacccctccc caaagctgaa caaggtctcc
     1681 gtcatccgta ttgctattca gaaaatcggc ggttttggtt gcggcggaag gcatccgagg
     1741 ttcatagttt cgccgataga catttgccat cggggcggag ccgatccaga aaacagctaa
     1801 taccaataat gatgccgcaa cgggtttcat gcggacggag agttggcgca gcatcgaatc
     1861 tcccaaccaa gccgccagaa tacaagcaga aggcacacag agaataaagt aatgctgata
     1921 attcattccg gacagtcctg ccaatataag ctcgaccggt aaagcaatca aggcgaacaa
     1981 taacaaacgg ttctgatcaa acagtttctg gcgatcattc cggttgcatt ggcggagact
     2041 gcgaatccga tcaaacaatg ccgcaggcca tgacaaaaaa gccaataata aaaacaccga
     2101 taacgaaggt aaaaatgtca gtgtcgtcag ccctgttgcc catcgactct ccccgctttt
     2161 ttgagcatag acaaaattat aaacaaacgc cacatcccaa tattctcgta aaattccttt
     2221 tacggcaaaa taaacgatcc agattccgtt cactgccaga aatccgatca gaaatcggac
     2281 ccacttataa atcacctgat ggacgaaggt agatttttga tcacgattca aataaagctg
     2341 aagaatatca agtaagaata atgccgccca caatccgacc gtattttgtt tgcaggtaaa
     2401 tgcaagcccg caaaggacgc ccgagagaaa tccctgccag cccaatcgat tccgaaatcg
     2461 atccgaaaaa gccaaaccga atgcagccaa ttgaaagaac aacgaaaatt cttcggtata
     2521 gttaccgaaa tcaaataaac gtgcctggtg caaaattccc aacatggtta ccgaaagggc
     2581 aatcccttcg gaaaaatatt tctttaagat ccggaaaaat aggatcgatc ccagcaataa
     2641 agcgatcact tccagcagcc aaatcccgat caggtgcccg tttgccagcc ataatccgaa
     2701 ggcatcaata taaaatatca gcggcggttt atgatcccac ccatccacat aaggtaattt
     2761 tcctctcaaa atctgctgtc cgatataact gaaaatcgag gaatcaaagg aaggataata
     2821 attcgctcga atcggaataa atgccgacat caacgtcagg caagccaaga aaattagaac
     2881 aacttgccaa acgattcttc caccccaaaa taactgtttg aaagcccgtg agacggaacc
     2941 gttttcctta gaataataat aaacaatgcc gataaaggtt tgtaatgaca aaattgccgt
     3001 cagcagaata aaaggcaacg cctgcttcag gtaagcgacc gtaaaatcaa gcgctgggtt
     3061 atcaaaataa aatcgggttg taaacgcgat aaagaaacaa gccgcagaaa gaaaaaagca
     3121 agctgtgccg attttctccg accgccgcaa gatttttgat ctgaaaagcc aacccgataa
     3181 aacaacaaca acgatcagcg caatcagaat catgaagatc aaccgcagtc gtgaaatccc
     3241 acccgattcg gcgggtttcc aaagaagtaa acccgcaaaa agagactcaa gaatcgatag
     3301 aacgggatag agcaatgcca taatcttgtt tcaatcagcg ggaggcaacg gatcgaagtg
     3361 atagatcagc tccgaatggc gccgcgcatt ttccgccacg gcggcaaaat attcggctgt
     3421 ccgaattccc gcggttccgt ctgtaaaagt acattcatcc gaaacaaact gttttcgctc
     3481 cgcggaatcg gcttccggat gagccaaata aaagttcaga gcttccgtga gttgttcttc
     3541 gttgtagacg acttttcccg cttttgcggc acgaaatcgc tcatgggtcg gccattctcc
     3601 gatttccgtt aaggataaac tgaactttcc cggctttttc cacccacccg gtgcgtcgat
     3661 acacacactg ataatcggcc ggtcatgaat agcggtctca actaccatcg tcgaatagac
     3721 ggtgcaaaat acatccgacc aagccatcat cgaatctttt tcaggcatat cctcgccgga
     3781 atagaaaccg agatctccgc ccaacgcaac cggttcaacc aaatgaatat acggtttatc
     3841 tttgatcaga cgacgaatcc gttcggcttc ttcttcgaac agtgaacccg gaataaaatg
     3901 gttgggatgc agacgaatta ataattgagc gggataagat agtttctcct gagaaatgat
     3961 attcaccaac gcttcaatat cctgataatt cggcgaaaaa gtcacaaagc tgcaggcaaa
     4021 cgaaatcagt ttgcgatccg gatcaaggtt gtgatatttg aaatatgttt cacggctgag
     4081 tcgccaaatt ttgcgaaaat atccgtcgta ggtgggaatt ccaccgacat ggactcgttc
     4141 cggcagccaa tcggctccga gtgtcaattc ttgtttttga atttccgacc aacaatttac
     4201 ccattgcacg ggtgctcccg gcaatcgata actgctggga ttatcccaac cgatcacagc
     4261 gaaagccgtt tcgattccgc gcgccgccgc ctcgcgtaaa agataacgat ccaaccgcca
     4321 acctgccgta ctcgctataa ccagattcgg ttgatatcgg tcaaataaat ccccataaag
     4381 attggtggta tagcgattct gtttgcgcac caatctcttt cgcgcatccg ccgatttccg
     4441 taatttacga atataggttt taataaacgg catcagcaat tttcctcttc gggatgtctc
     4501 gaatgccatc tggcgtaaat ggctgtcaat cgcatgggta ttgacttgat cggaacctcc
     4561 catccatctt aaaaaatgaa tccagtattg ttgattatga tcaaccgttt caaaataaat
     4621 cttcgcatct ttgagtcgca atccttcgaa gaacaggttg gaagattgga atctttccct
     4681 cattttttcc gtcacgccgt catccgttag aataacaacc cggaagcctt tctccaataa
     4741 cgttttgacg acatccgttt gaagaaaata aacgatcgcc agcccatgat cggcactgat
     4801 catgatcgtt tttgtctgat tcgtttgatc cattatcttt cctttttccc gcattccgtc
     4861 gcctatcgtc ttaagaaaca ggtattcctc ttaaaactcc ccgtggcgat cttatttttg
     4921 tctgtcatca tagtaccagc ggaaaatttg attatataat cccaacagag tttttttaac
     4981 cggaggcata tccaacaagc gattccgttc ggctgttttc tttgatcggg ccttttcttg
     5041 aaccgcatct gccaaagtat tactcatatt catcacatac ggctgaacag gcatcaaacg
     5101 caacagccct ttctcattca ttcttaaatc caactcttta acctgtccca ttggacgatc
     5161 catttgaaac ggcaaaaact cagataacac ctttttatat gcggtgaact gccaatgcga
     5221 ggcaccggcg attgcttgga caccgtgata ggtcagcaaa tgatcgcaag tggcttcata
     5281 atgcgaacga atttcctctt ccgtttgccc caaactcaga tcaaattcgc ggaacacttc
     5341 aaaagggacg aattgtccgg attccaattt tacatcttcc gtcttttcag cccaatccaa
     5401 tgttgcggta tagaattgag ggtttgtacg aaaaggacgg gctgtcacca ttccgacatt
     5461 cggataggtt tcaaggattt caatcgattt ggaaagccat ccgggtgaaa aaagacaatc
     5521 attatcggca taggcgataa tctctcccgg agcgccattt aagataatat tccaagcccc
     5581 ccctttcccg agattttttt cggataagaa aaggtaccgg attcttccct gttccaattc
     5641 atcccgcaat aaatctttaa cctcggggca gctgccgttg tcaaaaacca tcaaatcata
     5701 ttcgacgtct gcggtacttt cggcaatgct gtcaagacaa gcatgcaaga cttggggtaa
     5761 ttcggcgtaa aagccgctga taaacggaat gtaattcaaa atcgcgaccg ttacccgagc
     5821 cggtttcgcg acattgttaa caaattttgc cggattttga ccgatccgca taattttcct
     5881 ttttatttcc cattctcgtc aaagcccgat caagagctct tcacgaaaag tttttaaatt
     5941 cgaaaagatc tcaatctttc ttttttctcg agcgaaattc acgataggct ttttccggat
     6001 gaaacacggc aaaaccgacc tcgcccaata ctcttgattt caattttatc agtgggaata
     6061 tttccatttc ccgtaggatc tttttctttg ttcgaggagg acgtaccaaa tttccgttcg
     6121 actccaattg ccaatcacaa tccaaaagcg tgaaaatcgt gcgcttccca ccggctaatt
     6181 ccacattatg tttcgattcc ggcaaccgat aatgggattg accgttaggc aaatgggcgt
     6241 aatcatgatc ctgatggatg atatcgatcg cttttgtaca gtcgatcagc ttccagcctt
     6301 gtttccgtgc ttcataaaac atccaattat cccaacccga acgtccgatc gcaaaatccg
     6361 ggattttttc gaaacaagtg cgcgggaaaa tgaaataatc actgccgccg gccgggtgcc
     6421 tgcgtccgcg atctgccagt tctttccgaa gctttccttg ccaattattt gaaaaatcaa
     6481 tttttcgagg tatatccaag tcccaacgct gcccgactaa aagaaatttc ttttcctttt
     6541 ccatcattcg tcgagcttgt tcgagaaaat ccgggaagag aataatatcc gcattcacgt
     6601 atgccaaaag tggagaatcc gaagtatctc ttgcgagtgc gaaaatatcg ctcagaagcg
     6661 gcgtaccgga ttcattgcgt tttacctccg caatatatcg cactcccaat tcttcggccg
     6721 cttcccgaac gccgatatct tcgccgatca aaacaatgtc tacatcctct cccaattgtt
     6781 tccacgattg aatggcattc cattgaatcg ttgcgatatg ctcgcgatta aacggtttgg
     6841 gagagataaa aatcgtcatg gtcgtcataa tgatttttta tccctgagga ttcgctcgtc
     6901 gattgcatca acctgtttgg catgggttgt ttcgtttatc tgttccaatt cggggaatcg
     6961 ctcaaccagt tcaagcactt ccgtccagga tgcatgcaac ggatcgggta accgccggaa
     7021 aatttcctgt aataattcga aatcatccgg cgtatcggca gtccatcgat gcattccgca
     7081 atccgggtca tgatccagaa tcgccacctt aaaacgacct tcgggggtat aaaaccacgg
     7141 catgacatgt tcgcgctcga aaggtttatc cgcttctttc cacgctctct ccaataaagg
     7201 aaatgaaacc acttcgacgt ccaatccgat gggaaaagtt cgatgccaag gagggggtaa
     7261 acgattcgcc gcgaaatccg ctccggtcgt ttcatataac gagacgacat catcaattac
     7321 attcggatcg atcaaaggac aatccgcggt aacccgcacg atgcgatccg tccctctcca
     7381 aagagcggtt tgataataac gatcgaggac atcataagtc gatcctcgat aacagggcac
     7441 cgagttgact tcacaccaat cggcgacggc atcatcggaa ggatcggtcg ttgtggcgac
     7501 caccgtttcg tcaatttttt ttgcctgttc tccgcgcttg ataacccatt ccagcatcgg
     7561 cttaccggat aagtttttca tcactttccc gggtaaacgg ctggatccca ttcgggcttg
     7621 aatgatgagt gtcgtcttca tttgttattt cgaatcgtat cggctgttac agaccgtaac
     7681 ggcattccgg taagcttccg tcagccgata tttattcttc tcccaatccg ctttttcttc
     7741 gatcagtctt cgtgcgggag cggtaatatt ttgcatttcc ccacgatttc gatagcagaa
     7801 aagaatttta tcagccattt gggcagaatc gcctgtttta aacaaaaaac ctgtccttcc
     7861 atcctccacc cattccaaat ttcccggaat atccgaaacc agaacgggaa tttcacatgc
     7921 catcgcttcc aacagggata cggaagatcc gtctgtgatc gatgcactta aataaagatc
     7981 cgccgcccgg taataatcga ttaattgatc attcggcttt ctgccgagcc acaaaatgcg
     8041 atctctaatt tctttgggag cagcatccgc aatatttcgt aataagttga attggctgcc
     8101 atccgctaaa attgccaatt tcagtttcgg ttcttttgcg gcagcggcga taaatgcgga
     8161 aagtgtaata tccacccgat aattcggttc aagggaccgt gtactcagca acaggaattc
     8221 atcttcttca gccaattccc gccgaacggt actgttccct ttgcaaaatt gcttgggatc
     8281 gattccccaa gggaaaatcg tcgcatgatc ttttttcagt cccaaaggga ttgccgtatc
     8341 tcgttcgaca taacagtcac ccaggaacca atcagcttga tgcagggcaa tcgcagcgtt
     8401 ttgtttcagt ctcggatccg cttcgatatc atgcatcaga tcgaaccccc atgacatcgc
     8461 cgcatgcgga tgaaggtccg cttcggctgt caaccatgaa acatccgtta atggtccgct
     8521 atgaatcaca tcgggttgga tttggtcaaa aatctctttc agatccgaca agatcgcgtc
     8581 cgcatttcga ggatcatagg ggatttctcg ccaccgccat tccgcttcgt gaaccgcctc
     8641 cggcaattca cggctctcca aatttcttcc cgagttctcg agccgaagaa agaaaacctc
     8701 atgaccgata tcatgaatgg cttttagaaa acgataatca tgaggacaat aatcccgcga
     8761 aaaatagaga attttcaagt taatcttcca aatcctgccg ggttatttct tttccgtaag
     8821 ggagatcgga cagcactttt ttgccgatca ccgcggaaat ctcgtagggt ttcaaggcgc
     8881 cgggtgtcgc gggacgtaag acatcgatca tatctcgggt gagccgctca ccggctttga
     8941 tatcccgagc cgctctcagg caacgtcgtt gaataacggc tgtttccttt tcgttttcat
     9001 tgatcttttt ttccggtgaa cccaacgcgg cttccagttg gcgggtgttg acaaccattt
     9061 cctcccaaga tttcggattc atggcgaatt tatgatccgg accgatacgc tcattatcgt
     9121 cggtaaaatg tttttcaatg actttggcgc ctaatgcaac cgctcccaaa accgtcgcat
     9181 gtcccggcgt atgatccgat aaacccaaaa ttaaatccgg ccaaagccgg tgatacgtct
     9241 ccaaaacccg aagatgtata tatttgaagt tctcgagact ggcggtgtaa tttgtattgc
     9301 attgcatcaa aacgagctgc ggattgatct tcaaaatcgt ctcgaccgcc cgtgtgacat
     9361 cgctcatatc cgctgcgccg gttgccaaca gtaccggttt ctgtttggat gcgatccgct
     9421 cgagcgactc aagccagtct atttctccgg aaccgatctt atagacgggg atatatcgat
     9481 ccagccaatc aatcgattca aaatcataag gcgatgaaaa atacagaatg ccttcttttt
     9541 cacaggtctc cttcagttcc tcggaccacc ggtccggaac ggaagcatct tgataaacct
     9601 caaaaacact tttttgccat tttgcttggt gggatttctg tccgccgaga tttttaaatc
     9661 cgaaatcgga gacgattttc ggcgctttga aattttgaaa cttagcggca tccgcacccg
     9721 attcctttgc caaatgaatc agcattttgg cacggttcaa atctccgtca tgattcgctg
     9781 cgatgtccgc aataaaatat acggggtgat tctctccaac cgtttgattg ccgaatttca
     9841 attccataat tcctcttttt agctgttatc cgtccgaaaa gatcgaatca attcgggcaa
     9901 acctgatttt tcggcttcat aaaaatgatt caaacaggtt tcctggtccg gtaaactttc
     9961 tcccaaaaat tcccgaagtt tgtcggtatt cataatcaga taaggagatc gcggtgcatc
    10021 caacccgccc gcttgccatg aaaccggttg aattaaattc ggatcaaaac cgaacttccc
    10081 ggcaatgttt actccgaatt cgtatttgct gagaaattcc gggctgaaaa catgatagag
    10141 gccctttgca ttcttaagca gcagtttctc caatatttcc gccaaatccc agacatagag
    10201 aggacagaaa aagacatcgg taaacccgaa aacccgctgt ccggatttga gatttgcata
    10261 aaaaaactcg cttaagctgc gatttccgga aacactccag ccgtaaaaat tgacacgagc
    10321 gacgacggca tcggggttcg cctcgaaaac cttttgttct cccaataatt tggaccgggc
    10381 ataaacattg atcggatgag gaagatcgtt ttcggtatat ccgttttcct ttcgctcttt
    10441 gccgtcgaac accgcatccg tggaaatatg aatcatcgga atatgccgtt ttgcggtttc
    10501 tccggccaat ctgccgggaa attccccatt cagttccatt gctgcttccg gattcttttc
    10561 acaggcgtcc acattggcca tcgcagcaca atgaatgacg gcatcgggtt gaacggtatc
    10621 aatcaattcg gcggcaacat ctttccgaaa aaaatccctt tgaaccattt cgaagggaat
    10681 tccttgcagc gtcagatgat ttgccactcc gaaaatttga tattttttgc tgagccccag
    10741 cgccagattc atccccagca agccggatgc tcccgtaacc agcaattttt tcatcgttaa
    10801 aattcttctt gatcgtaaga ttccataaag ggcgcgatca tttttttaat cccttccaaa
    10861 tcgagccatt cggtattgtt attactggaa taatgatagt catccggcaa ttttttgccc
    10921 aatttttccc aatccctgcc gaaccaagtc gtgcccgaag gttggacgat aaacatgtca
    10981 tcgaattcaa ccgtatttcg tgcttcgtct tcggaaatca aaacctcatg aagtttttct
    11041 ccgggacgaa tgccggtgta agtaatttcg gcttccggag cgacggcggc agctaaatct
    11101 tttaccttca tactgggaat tttcggcacg aacacttccc cgccgtgcat ttgttcgata
    11161 catcggatga caaaatgaac gccttgttcc agagaaatcc aaaaccgggt catccgttca
    11221 tcggtaaccg ttaatcgtcc cgttgcccgc tgtttcagga aaaccggcac aaccgaaccg
    11281 cggctgccga ccacatttcc gtatcggaca caagaaaggc gcgtttcccg cccgccggca
    11341 tatgagttgc tttgaatgac caatttttcg gcggctaatt ttgtcacgcc ataaaggtta
    11401 accggattca ccgctttgtc cgtactgatc gccaggactt tttttacgcc catatccaat
    11461 gcggcatcaa tcacattttc cgtacccaat atatttgttt tgaccgcctc aaacggatta
    11521 tattcgcatg ccgggacctg tttcaaagcg gcggcatgaa cgactatatc aaccccttga
    11581 aaagcccgtt tcatacgatc caaatcccga acatcaccga taaaatatcg caagcgcggg
    11641 tcattgatcc cggaattttg catttcatac tgtttgagtt catcccggct gtaaataatg
    11701 attttttccg gatgatattc cttcagcatg acctggatga atttctttcc gaaagatccg
    11761 gtccctccgg taatcaatac aactttatct gtccaatcca tgaatttcct ccgaggggaa
    11821 aatctttcgc cgagaaagcc ccggatgatt ttccctttta ggggcgctaa gctcccgtct
    11881 attttaattt tctttcgtca atataatcat cggatattga ccgtattccc cataatattc
    11941 cggttttttc tcttccaaag cgaataaatg tcgtaaggcg gcttttccgt aaggattatg
    12001 aattaaagcc cgcagccaac ggacattcac gctgcgccaa caacgaattt caatctttga
    12061 gcggccgatt acggcgggta attccgatcg aatccaattt gcatccaatt tttgaacata
    12121 agtaccggta ggtgtttcgg ccgctttttc ggatttttcc tgccgggttt ttccgttttc
    12181 tttccctctg atacgggcaa tccggtgccc gattttttct gcccgcagga cccgtttctg
    12241 ccaatgtttc atcatcgccg attctgtcca cccattaacc acaaccgctt ttctcccggg
    12301 cagtaaaacc cgattgatct cactccaagc tttcttttga tcttcaagag gcaaatgatg
    12361 aagcgtatgc aaagaaaccg ctcccgcaaa aacctccgat gaaaacggca aattggcgac
    12421 atctccgaca acaaaaagac catgatcgcc gatccgtttt cgggcttctt ttaatgcggt
    12481 aatcgaaata tccaggcaga cccgaaattc aaacttttcc gaataagcca gatattcccg
    12541 atactgaatc ggacccgaac ccgcgtccag aaaatatttt ccgccgtttt ggaaataagg
    12601 taccagacgt aaatgacagc gatgaatata atccgccgaa accggcctta aatcttcata
    12661 tcgggcattc tgatagaaac cgtccgattc cagtttccat ccgatttcat cgtaaaaatg
    12721 tcttacctca tttttgggag cggatccgct cattccacat ccgtttcatt ctttttctca
    12781 gctaaaatct ttttccaatc catcggcttt ccgatcagat aatcaaagct ggatttgtat
    12841 tccgcttgac cttctttact caacacataa gaaaacggcc gtttgtcata atcatcccac
    12901 attttcacca aatgccacgg gaaaggccgc ggataatcaa aaaagaaggt ataagcataa
    12961 gtccatgcca attttacctg ctcttccgtc aaaatcgatt tctgcggatc atccagaatg
    13021 gaacccaaga ttttatagta gttcacccag ttatcgggat cataagtgaa tcctctgccg
    13081 cgataatggg tctgaccggc aacaatcacc ggtacgccgt tcatcgccat ttccaatccc
    13141 atcgttgtgg tatagacgag cccgacggtt gccacatcca ccaaatcata agaattcgtt
    13201 ttatcggtcg gggtaatcaa gtgtatatgt tccggcaatc gcggcaataa gtcatgaacg
    13261 acatccacca tggaagtccc gtgagttaat tgttccccgg gatgcactcg aatcaccaac
    13321 tgggaatccg ttcgttccac aaaatactga acggtccttt caatccattc cgccatattc
    13381 cgggaaaatg tctgtcgtcc gagcgtcaga ctgtccccca atacatttgt cgccataaaa
    13441 acaatcggcc ggtcatccaa cttcagttct tgtttaattt tttccgtacc gactcggtca
    13501 ctcccttgcc atcgacgggt aaagttctcc caaatatcgg catgctgtcg tgccgaaaac
    13561 atggctttca ctttctttaa ttcttcggtt tccaccggga tattcttgcg aacttcccac
    13621 agatcgtcgg tctcttgtcg cataacttcc cgattttggg ctatccaaat tcgtttccgt
    13681 tgctcaccga attcatatgt gactaccgga atttttaacc actttgccat tcggtaagcg
    13741 atccccattt cctgaatcgt cccgttcgga atgatcatat gatcgggttt attggctgac
    13801 agccaaggat ataacattct ggcgaaactc tcgtttcttt cgagtcgcat tttataaaga
    13861 ggatgttctt tgtcaacgtc ctcaacctgc aaggtatatt gggcatcgta gcgagagact
    13921 tcttcgatga tctgtttcaa ttcatccgga atcggtttat aggaaggatg gatcggcaga
    13981 aaggaaacaa tatccatata ttccgccgct tgttcgaaaa ccttttttgt atataaatcc
    14041 tgtcttctta aatcaaactt gttgacatca ttctgccaat ccgaataagg aagatagccg
    14101 aacgtgacgt gatgcccttg ggcagcaaaa taaagagcga tggtcgccgc ttcttcaatc
    14161 caataatgaa gtgacgtaaa tacaagtatt ttttcaggat gagcgttcgg cgttttaaat
    14221 tgatcaatat cggccaacat ctgcggaaga cgtgcctgaa tatttttgag cgaataccgt
    14281 gtcccgggtt cgctctgttt tttcaactga aaataaaatt cagcggtaag gggaatttcg
    14341 cccaaaattt tactgatcgt ttgtttatag tttgattgca ttagtcaatt ccctctattt
    14401 cccaatttaa tcgcggtcgg atcatgcccg atcgtgtttc aggccattgc gtccccggcg
    14461 cacccgttcc atccacatta aacgtcagag cttgttcttc gtgaaaatag cgtttaatct
    14521 taaaagtact ggcattgact ccgatcacat attttccttc atttaacaag tccgccggaa
    14581 tagtgcaacg gctccggtat ttcccttttt ctcgggtacc gtactgagta aatttttccg
    14641 gatcatccac atcaaaactg gtaaatataa attctccccg ggtactgatt aagtaaattc
    14701 cgacccgcaa gccggtaatg ggagcatcca gccggtattc aaattcaact tgaaaaggtt
    14761 cggtcgaacg caccgtttcc gagacatggt ttcgatcagt caaaattcgg attgccagcg
    14821 gggtgaacgg agccgcatcc ttcgaaattt cctccgctgt ccagaatcgt tccccaccct
    14881 gagaaaatcc gtgagacaga taataatcca ccgcttccac agagggagct cgcatgacca
    14941 ttttcccttt ctcaaggaca atcgtctcgt tcgttaaccg caggatcgcg gacatattat
    15001 gactcacgaa gaggattgtg cgaccttcgt tggtaacatc ggtcatttta cccaagcact
    15061 tccgttgaaa ttcggcatcc ccgacagcca aaacctcatc aaccaccaaa acttccggtt
    15121 ccaaatgagc ggcgacggca aaagccagtc tcagatacat tccgctcgaa tagcgtttga
    15181 ccggtgtgtc gatgaattct tccacttctg aaaaagcgat aatttcatcc aattttcgat
    15241 cgatttcggt acgcctcatc cccaaaatgg cgccgttcag ataaatgttt tctcttccgg
    15301 ttagctcggg gtgaaaaccg gtaccgactt ccaggagaga accgacacga cctttgatcg
    15361 acgccgagcc gaccgtcggc tccgtcaccc gcgaaagaat ttttaataaa gtactttttc
    15421 ccgcaccgtt ccggccgatg ataccgagtg cttgtccctg tttcacatta aaagaaatat
    15481 cttttaatgc ccagaaagaa ttatcgtccg tcattttccc gtgatgtttc gatttggatt
    15541 tgttgaatgg ttccgtaaga gtttcccgga aagtccgata agcggcagga gccgctccga
    15601 tccggtacct cttaccaata tcatgaactt ctacggcata atcattcacg gcaactccgc
    15661 ttaaataaca tccgcaaact gtttttccat tcttcggaaa tagaacagcc cgctgaccag
    15721 aatcaaaatg gaaataacgg cacttaatat catgctttga atcggcaatt ttatatattc
    15781 acccaaaatg gaggaacgaa atccgttgac aacaccgacc atcgggttca aattataaat
    15841 aaattgcagc ttggggttcg aaatgattcc cgatccgtaa gcgatcggcg ttaagtacat
    15901 ccacaactgg ctgataaacg ggacaatgta cccgatatcg cgatacatca catccatcgc
    15961 cgaaaaccat aagctgacac cgaatgcggt tattacggtc agaagcaaat aatacggaat
    16021 cagtaatgca tagatattcg gagaatactg ataataaatc ataaatccga tcaaaatagg
    16081 caatgcgacg aaaaagtcca caatacaagc cagcacgctt gacagcggaa ggacaattcg
    16141 cggaaaatat atttttgtga tcatattccg atttgctacc aacgatttac tggcattccg
    16201 caaagaattt tcaaataatt gccaggggag aaggcctgcc aatgaaaaaa gcggataagg
    16261 gatcccgtcc gaagggactt ttgccaagtt gccgaagaat atcgaaaaaa caaccattgt
    16321 catcacaggc tgaataatcg cccaggcagc tccgaggatc gtctgcttat aacggacttt
    16381 taagtcacgc caagtcataa agaaaatcaa ttctttataa tgcttcagct ctttaaaatc
    16441 gattattttc caaccgttgg atggtttaat agttacacgt acatcctctg gcgaggtttc
    16501 aagtcccaaa ttccctgctc cgttttaatt ttctttaaga atggcatctc gattcgagat
    16561 gtaataatca atagtctgct tcaatccgtc ttcaaaactc atctttgcgg taaaaccgaa
    16621 gtattcccgg gcacgagaca cgtccaaagc ccgtcgcggc tgcccgttcg gtttactggt
    16681 atcatagacc aatttacctt catatccgat taagcgtgcg atcagctcag ccaaatcttt
    16741 gattgaaatc tccataccgg aaccgatatt caccggttcg ggaccgtcat atttttcggc
    16801 tgccatgacg attccttcgg cggcatcccc cgcataaata aattcgcgcg tgggtgaacc
    16861 gtccccccag atcacgacct ctgaaatatg attcagtttt gcttcataac atttgtgtat
    16921 caacgccgga atcacgtggg aagatttcgg attgaaatta tctcgcgggc cgtaaagatt
    16981 taccggtaac agataaatgg aattaaatcc gtattgtttt cggtaagctt gggattgaac
    17041 caataacatc ttttttgcca gcccgtaagg cgcattcgtc tcttccggat aaccgttcca
    17101 taaatcatct tctttaaacg gaatcggcgt atatttcgga tatgcgcaaa ttgtcccgat
    17161 cgctacaaat ttttccactc ccgctttcca ggattcatga atcagttgaa cacccatcat
    17221 caaattatcg taaaaaaact ccgccggatg ttccatattg gcaccgatac cgccgacatt
    17281 ggcagccaga tgaataatga catccggttt cgcatcggac aacattcttc gaatatccga
    17341 aacctcaatc agatcatatt gttttcgcaa agggataaag atatcggtaa cccctctttt
    17401 tttcaagctt tcctgaataa atgaccctaa aaagcccgct ccgccggtta cgcagatcct
    17461 tttattcgcc aaaggtgaag gttcagtcat cttgattctc cttattcctt ccgtcgagtg
    17521 gttgtgccga agcctttttc tcgggaaaat taatcgaaat ttgtaaaccg ttgacgctga
    17581 taatcggatc ttgctctccc cccgacgata cgatcttcaa cccgcgggaa acggcaatta
    17641 atcggcatat ttcagccgcg tccccgtccc cgtatacttg aatcgtcgta aaatgatcag
    17701 cctcacagcg atctaataaa tcctgcattt gtttcttaat tgttcggtag gcactgaacg
    17761 atcgctcaag gaattcggta taaagttgcc ggctcaacgc cgttccggat tccgtcacga
    17821 aataattcag ttttcgcccc tcaccgggaa ctgttctaag aaagccatcg gaaacaagcc
    17881 gtttcaaacg ccaatttacc gtaccgaccg caactcccaa ctgctttccg atctccgctt
    17941 gagacagctg cggattttcg gccagcaatt tcagaatatc aaggtctttc gaatcataag
    18001 cggtttcata agtcataggc gtcgaatatt caatgactga attatattca aaatcctaaa
    18061 ttggtcaagt taccaacttc tataacgtta tgtcaagact atttatcacc tttttatagt
    18121 gattattatc ataatcattt cttaataagc tgcgttgagt tgaataattc tatttataat
    18181 gacagaaacg tcatttgaaa ggagttgaaa ctttgtaccg tttatcaaac actcaaaaag
    18241 aagttgtact gcaatggcaa cgttttttcc atcaaaatcc cgaaatcagt tttaatgaag
    18301 tgaagacttc cgaaaaaatc accgaaatcc tgaatcatta tgggatcgaa aatcacagag
    18361 tgggtaaatc gaccggaatc tccgctgaaa tcaccggcga gctgccatcc gattcaccga
    18421 aacggatcat gttacgcgcc gacatcgatg cattgcccat ggaagaacaa aatgatctcc
    18481 cctggaaatc tcaaaacccc ggtgcggcac atacctgcgg tcatgatggt cataccgccg
    18541 ctcttttagg agccgctgtt ttactaaacg atgaccgcgc gtctttttcc ggttccgttc
    18601 gtttgatatt ccagccggcg gaagaatcgg tcggcggggc gagcatcatg attcgggaag
    18661 gggaattaga cggcgtcgac ttggcagccg gcattcactt ctggccggcg ttgcaaagcg
    18721 gaaaaattgc ctgtgaatac ggagcggcta tggcgggatg cgaacatttt tgcattcgct
    18781 ttttcggaaa aggcggccat atcagtgtac cgcatctcgc aaacaatccg atcctttcgg
    18841 cttcggagac atttattcaa ctgcagaccg cagccgccca gcaattttca ccgttcgaag
    18901 agttgctgat cggagtcgga aaaatcgtcg gcggaaccca gtacaacatc attccggaag
    18961 aagcgctttt ggaaggcaca atcagagctt ttcaacccga gacactacaa aaagtccgtc
    19021 aggtcgttcg aaatacggcc gaacatatcg cggcagcaaa caataacacc tgtgatattt
    19081 ctttcggcaa tggaattcca ccggtcatga ataacccgaa agccgtagat ttggcaattg
    19141 agaccgcttc ggcgctgatc gggaaagaga atgttttgac agaaataccc aaacaattta
    19201 ccggagatga tttttctttt tatgcagtcg agaagccggc cgcatatatc aaaatcggct
    19261 cttgggaccc gtcgaggccg gaaacgaata atccgttaca cagcccgcgt ttcaatctgg
    19321 atgagaaagc gctttacttg gctgcggaat attttgcgaa ttacgcctgc gcttttttgc
    19381 ggaatccggc tgaagaatcg atttagatcc tcaattcgtg cccttcaaga tcggataaaa
    19441 aacttgtaat atttttcgtg atgattcggt cattgctttt ataatttttg ccatgaataa
    19501 aatgaaactt ttaaccgata gcaaactttg cttgtgtttg gggcgaggaa tactgttcat
    19561 aaccagtttt ggctgaattt gctgatattc agaaattggc gaacggaatt cctcgctttt
    19621 tacagttgag acatccgttc gttttttatt taaacggttt ctgaccgttc ggatgtcaac
    19681 agaaaggttt tatctgaaaa ggagctgcaa tgaataaaaa attttttctt gtcttttgcc
    19741 ttatgcttac ggcgatcctt ttttcgacgg tcggagcttt cgctcaaagt gaaagcaaca
    19801 gcctgactgt cgctctttcg gttgagcccg tttctctgga tccggcgggc ggattgtata
    19861 ttccggaaca attgctgatt caacaaatct atgatccgct gatttatgcg gaccctgaac
    19921 aaaatcttca tccggggctg gctacttctt gggaagttaa cccggaagga acagaattta
    19981 ctttaaacct tcgcaatgat gtgaaatttc atgacggaac ccctttcaac gcccaagcgg
    20041 taaaagtttc tctggatcgc gctgcggtcg gaaccactgt ggcagcggcc gcaccggcta
    20101 ttcttacgga ctatattgaa acgcaagtgg tagacgatac ccaagtggtt atcaaattct
    20161 cgactccgca tgcgaccttt ctgcaggatt tgacgcgccc gtggctgatg atttcttccc
    20221 cggccgccat cgaacaatac ggggatgatt acggacagca cccggtcggg accggcccct
    20281 tcattttcaa agagtgggca gcacaggatc atatcacgct tgtcaagaat cccgattaca
    20341 attggggccc ggaattcttc actcataccg gacctgcctt gttggacgaa atcgtatttc
    20401 gggttttgcc ggaagcggca attcgtctga ccgcatttca aaccggtgaa gcactgctgg
    20461 ttcaggaccc gtcctatctg gaagcttcgc agatggcgaa tgatggttcc acccaagttt
    20521 tcactttcac cgcccccgga atgacatctc atcagatgat caatgtcgaa aaagcgccga
    20581 ccgatgacat taatgttcga aaagcaatga tttacgccgt cgatcaggaa acaatcgttc
    20641 aaaccgcttt ttatggactg cagagtgcgg ctcacagcgt catctcaccc actacctggg
    20701 catttaacga ggaagcgaat aatctctatc gttatgaccc gaaaaaagca gccgaactcc
    20761 ttgaagaatc gggctgggtc gatacaaacg gggatggaat tcgcgaaaag aatggcgtta
    20821 accttcatat cgaatacccg gcattgcccg cttatgaaga ggcatttatg gagttgctcg
    20881 catactatct gaatcaggca gggtttgaag tgaatattac caagctggac gatgcgggta
    20941 tttccgaatt cggttatgcc ggtaaacata atattctgaa tatgggttgg atcagccgcg
    21001 atccgtctgt cctcagctat gtctatctct ccgataatat tgaaggcgga aatcaatccg
    21061 cttactcgag attccggaat gcacggttag acgaaatcct caagacggct ccgcagacaa
    21121 tcgatgccga tcaacgaaaa gcactttatg aagaagcaca aatgattatt atggaaaacg
    21181 caatcgctct gccgatccat acctacggga atgtttattt ggcggcaaag aatgtaaaag
    21241 gcttccgttt tgatccggaa ggttacccat acttttacga gatttctctc gcagctgagt
    21301 aaaggatggg aagttattta ggtcggaggt tattgacctc cctgtcaatc ctgatcgggg
    21361 tgtcaatttt aacttttctg atgttacagt ttgtccccgg tgacccggtt cgggctttaa
    21421 tcggaagaca aacagttaca cccgaaagac ttgaagaaat caaacaggaa atgggattaa
    21481 acgatcccat tcctgttcaa tattttaatt atttaaaaaa agtattatcc ggggatttcg
    21541 gccgctcgct gaaaaataat cgaccggtat tggatgcgat catggaacaa ttccccagca
    21601 cgctgaaact gaccgtcttc gctctgatcc tatccacatt tttcggcatt cttctgggcg
    21661 cgatatcggc ggcgacatat aacagctggg tggatcgatt cctctctttc ttctgtttgg
    21721 gaggtatttc tcttcccccg ttttttctcg cacttttact gattctgttt ttttcggtca
    21781 ggaacaattg gctgccggcc gtagccagca tgaatgattg gagaagtatt attctgccgg
    21841 cggtgactct cggtttcgga gaagcctgct ggctggcaag aattgtccgc tccagcatat
    21901 tggatgaaat gaacaagaat tatcagaatc tggctcgcgc aaaaggtgcc agtaactgga
    21961 caattctatg gcgacatgtt ctgcctaacg ccagaatccc gattgtcacg gctatcagta
    22021 ttcaatttgt ttatctgatg gcgggatctg ttgtggttga atcgattttt gcccgtcagg
    22081 gtatcggcag gcttgcggtg acggctattc agaatcgcga ttttccgctg gttcagggaa
    22141 cggttacgat tattgcagtg gtttacgtta tcagtaacac gctgaccgat attttctatg
    22201 ccatggctga cccgagggtt cgtttgcaat gaatgaaatc aaatttatca cgcagaacat
    22261 tgaaaccaga cggccgagca ccataaaact tctgctgcat aatttcaatt tcgttgtcgg
    22321 tgggattata ttgatcatat tgattatcaa tacgattttt gcacctttga tcgcaacgga
    22381 aaatgtaaat caggcaaatc ccactcagac gtttcttccg ccctcgaatg aacatttgat
    22441 gggaaccgac aatatcggtc gggatatctg gtcccgtgtc atttacggcg gccgggtctc
    22501 attggctgtc gggttggcgg ctatgatcat cggaatggta ttcgggaccc taatcgggat
    22561 cgtcgcgggt ttctacggat catgggtaga cagcctgctc tcttgggtga ccgaagtgct
    22621 gatggcattc ccgggcattc tgctcgcctt gaccgttatg gcgattttag gaccggggct
    22681 ctccaatgtg atcgtcgccg tcggtgtagg ctcgattccg caatttatgc gcatggcccg
    22741 tagttcggtg atgaaaaccc gtgagttgga ctttatcgat gcagcacgca ccatcggctg
    22801 taccgacgcc caaatccttt tcagacatat cttaccgaat attttgcgcc ctttaattgt
    22861 cctggccact ttggggatcg gcggcgcaat cttggaaggt gcatctttga gctacttggg
    22921 attgggcgct caaccgatga cgccggaatg gggctccatg ctttcaaacg gcagagcata
    22981 cctcggcaat gcgtggtgga tcagtgtgtt tccgggaatc gggattttcc ttgccatttt
    23041 aagcatcaat atgatcggcg aaggattcaa tgaagtttta gatccttcaa acaggcaata
    23101 atactcaaac gataaagcgc gtcggaaaat ggcaagtctg attatcaacg gaaaaataaa
    23161 aagggtcgaa ccggatgaga caatcgaaac ggcgatctta atcgataaag gtcgtattct
    23221 tcaaattgcc gatcccgatc gaatcaattt gaagatttat gatcgaacgg aaatcatcga
    23281 tgcaaaaggt gcggtgattt tacccggttt tatcgatgct cacaatcatt tcacgatgat
    23341 gggaacccag cgatctgaat tggatttaag cggcatccga aatcgagaag atattatcga
    23401 aaaaatacgg caggaaaatg caaaactcga ccctcaaatg cctattatcg gattcaacta
    23461 tgaatttgat ttcatccacc aggatcatcg cctttgcgcc gctgatttgg aagccgttgc
    23521 ccccgaccgg atgattcaaa ttagtgaccg cggcggacat ttgtccgtca caacaccatc
    23581 aacgttaaaa aaagccggaa ttcttttgaa ttcgcgtgaa tatgcggatt gcatttttcc
    23641 ctgcggagga aatttcggcg gttttaccgg cgaaatctgc ggtgtggcta attcgctttt
    23701 aagcgattat ctgcgcattc aattcagaga tgatcaaacc ttgaaaagag cttggaaaca
    23761 ggcatcgaat atcgcgacgg aacatggagt gacctcaatc catgccttgg ttgtagagga
    23821 agaatttgaa aaactgattc aatttcaaaa tcagttaccg attaaattaa aaatatatac
    23881 agagactaaa aattgcaaag cggttaaaac cgccggactg aaacaggtcg gcggttgcgg
    23941 acgggtgatg gtcgacggcg ataccggacc gtataccgcg gcattcttag aaccgtatcg
    24001 aaaccgaccg aataccaaag gcttgcttta ctattctgac gaagaactcg aagattacat
    24061 ctggaatgcc cattccgccg gtttgcaagt cgcattgcat tgtatcggcg atgcggcttc
    24121 ggaacaattt ctaaatgcca tcgaaaatgc acaacagcga aatccgcgcc cgatcagaca
    24181 tcgaatcgaa cattttgagt tcggaacctc ggaacagatc actcgggcga aaaaaatggg
    24241 cgtcgctatt tctcttcagc ccacatttaa tcattattgg ccgcatacaa cttacttttc
    24301 ggatttgggc gaagaaagag cgatgcgcag cgacccgatc cgctccgtca tcgatcgggg
    24361 agttccggtc gggttcggat ccgattgtcc ggtaacccca tgtgatccgt tgttgacaat
    24421 tcacagcgcc gtaaatcatt ctcttcctca tgaaagaatc agcccgaatc aggcgataag
    24481 atgccatacg ctgggaggag cttatttggg gaacgaagag acagaaatag gaagtatcac
    24541 agtcggaaaa gcagccgatt tggttttctt ggcggccgat ccggcgcaag taatcccgga
    24601 tgaaatcaag gacatacccg tattaaaaac gatcataaac ggagaaacag tttattccgc
    24661 cgactaattt ctctctttgc ttgttttttc ccaatcaccg attgggtcta ttgtcgatga
    24721 atcaaataag ataagtagat catgatcgag ccggctgaag cggcatttag ggattcaatc
    24781 cggcccttca taggaagacg aaccagctga tcgcattgtt ttctggtcag cgggcggagt
    24841 ccttctcctt cggagcctac cgcaatggca aggtttccgc ccaatttttg cggatctgcc
    24901 aaatccgatt ctgctccgcc gtccaaaccg attacccaca caccctgttc gtgcaaccac
    24961 cgaattgcct gggataaatt caactgcacg atcggaatat attcggttgc accggccgaa
    25021 gaaacggtaa ccgccggagt gacacccgcc gcccgagacg gaggaatgat tatcccatgc
    25081 attcccgccg cttccgcagt gcggattaag gtaccgagat tttgcggatt ttgtatcagg
    25141 tccaatagga gaacaaacaa gggttcttgc ttttcggcag ctctgttccg aatatccggc
    25201 aaatcggaat aatgaaatcc gctgacttcc aacgccatcc cctgatgatt ttcatcgagc
    25261 gaatccaaac ggctgcgttc cacttggatc ggaagtactt ttcgcttttc ggctaaccga
    25321 ataatttctt ccagtcttcc tttaaccgtt gcgccttttg cgatttccaa gcggaaaact
    25381 tcacggctgc ccgctctcag tatttcatag gcggcattcc tgctggtaat ccattctttc
    25441 attcgctaac caagcctaag cccaatgcca acgggttcca tctttgctgt cttctaagac
    25501 aactcccaat tccgctaacc gatcgcgaac tttgtccgag agttcccaat tcttttgttt
    25561 ccgtaattct gtccgcaaat caatcagcaa ctgaataaac ggatcggcag ccgcttgatt
    25621 tcgatccgct ttgggttcgt tcagaaggag tccgaaaacg tcggttaact cccgcaacat
    25681 cgcttgtgcc ggttccaatt ccgcatccgt ggcacccgca tcgcgagcaa tattaatcat
    25741 tcgaaccaat tcaaataatt gccccaaagc acccgccgag ttaaagtcgt catccatcga
    25801 tgcgatgaat ccttctcgcg tcgcttcaat ctgagatcgc aattcctccg aaacttcctg
    25861 cggagcgcct gctgcggaag ggtttgcggg ctggaacgcc gatcgcaacc gctcaagccc
    25921 tttttccgct tgaagaagaa cctcttcgct gaatatgatc ggattccgat aggccgagtt
    25981 caagatcagg taacggaaag aatccgcact gtgatgttcc agaaactcat cgatactgat
    26041 gatgttaccg atggatttgg acattttttc accgcgcaat tgcaacattc cgttatgaat
    26101 ccaataacgg gcaaacggct tccccgtaaa gctttcgctt tgcgcaattt cgttttcgtg
    26161 atgcgggaaa atcaggtcgt tcccgccgcc gtgaatatcg atcgactccc cgaaataatg
    26221 gtaattcata gcggaacact cgatatgcca tcccggtctg cccggtcccc aggggcttgg
    26281 ccaagaaggt tcgccgggtt tggcggattt ccataacgca aagtccaacg ggtcttcttt
    26341 tcgatcatcc gcttccttcc gaacaccgac cagcatatca tcaagctgtc ttcccgaaag
    26401 ttttccgtat tggctgtctt ttcgaactcg gaaataaaca tctccgtcca aagggtaggc
    26461 cattcctttg tctattaaac cttgtaccat tttaataatc tgatcgattt cctgggaagc
    26521 ccgcggatta taggtagccg gcttaatgtt caaatccgcc aaatttctct taaaatcttc
    26581 gatataccct tcggctaaac ggaaaggatc gacatgcatc tcattcgccc ggttgatgat
    26641 tttgtcatct acatccgtaa aattcatggc aaattgaact tcatatccgc gatattccag
    26701 atatcgccgg ataatatcaa aaaccaaggc agacatcgca tgcccgatgt gcgctttatt
    26761 gtaaacggtg ggtccgcata catacatctt taccttatgc ggttccagtg tctcaaacgg
    26821 ttcttttttt ctcgtcagcg tattgtatat ttgtatactc atagggttgc tgttccattt
    26881 ctgtttcagg cttggcaaaa atcatccttc cgattgccgt ttgatgaatc tttgtcacca
    26941 taacgctgat ttcttgtccg atatacatgc gaccgttttc cacgacgacc atcgtcccat
    27001 cctctatata tccgaccccc tgattatatt cttttccttc ttgcagaacg tgaatcatca
    27061 gcgtctcacc cggaagtatg actgatttta ccgcatttga gagatcattt atgttcaaaa
    27121 tggttatccc ctgcaggttg gcaacctggt ttagattata gtcatccgta agaatgggac
    27181 aattgatttt cttcgccaac gcaatcaatt tcccgtcgac atcacgaata ttttcaaccg
    27241 ctatatcgcg aatttcgacc tgagccggaa attctttttg aagttggcgc aatacttcca
    27301 gaccgcgtcg tccccgctgt cgttttagta tatcctccga atcggcaata aattgtattt
    27361 cctggagcac aaaattcgga actaccaatt ttccctgtaa aaaaccggtt cgtacgattt
    27421 ccgcgattcg cccgtcgata atcgcactgc tgtcgaccag tgtaattttt tcgttttcat
    27481 ccgactcttc tggttcaaaa aatcggggcg gcataaactt tcgaaaccaa tttatttttg
    27541 atatacccgc atacaccccg agatagccta gcagcaaagc gatcaggaaa ggcagaactt
    27601 ctcccaaagg atccggcaaa aatgaagccg gaatcgaaaa aagcgccgcg gcaaccaatc
    27661 cgaagattaa accgatggta gccgcagcaa ctttctcgga agattcattt tcaagcttaa
    27721 ctcgcaatcg ccgaaccgga tgaacaataa gaggcacaac aaggaaaaag cccaaaacgg
    27781 ctccgataat tgccgaaaca atcagcacct ggataggata caaatttgaa gttgttacgg
    27841 aacgggaaaa ataataaccc aaccacccgc ctaataaacc aaatgaaacc gctccgattg
    27901 tccgattttt tttgtcactc tggttcataa gttttccctc tgaatatatt ccttaattta
    27961 aaaaaagaat atagaactct gacgggccta aatctttttc gacttattat ttggtttaat
    28021 gattccaatt aaaaagtaat acaaatttcg atctcttgtc agacaaatat aataacaatt
    28081 tataataata tttttattat aacatatcct tattccgccg gacggaatat ctgtaaattc
    28141 aaaaccaaaa acgctctgaa agctgccgtt taatcggaaa agcttggggt tatttctcgg
    28201 gaaccacaat acctgtgaat tttctcgaaa cccgtctatc accgtaaaat tcgatactgc
    28261 catcaacgtt tcagagaaaa agcttcgcca aacgaattct cttcgaattc cgttgaaatc
    28321 acctcttatt catcgatgga gatcttttgt aaccaggatg catccttatt cttccggtaa
    28381 agcaccgaaa acccgattgc ggcgatcaga caataaaaac cggcactgta ccaaatcgat
    28441 tgcgggataa attgatcata taataagccg gccagcgccg gtccgaatgc attcccgatc
    28501 ggatgagaaa gattgtaaat actcatatat ctcccgcggg cgtccgccgg tgctcttttg
    28561 gcagttaaag ccagcatggt gggggacatc aataattcgc cgatcgtcaa aattcccatg
    28621 cagatacagt accaaattac attcgcacaa acgacataca agagaagccc gactgtataa
    28681 aacagcgacg agatcaccat aaccagcatg ggatgaattc tgacggtaaa tttcatcgct
    28741 cccaattgca agaatacgca catcaaagca ttcactgtaa agacatagct gatttggctt
    28801 tccaaaattc cgtaagtttc cgaagcatag agcgaaagca aattaaaaac gatggatgaa
    28861 cccatataaa taaagaccgc cattaagatg gtcgagacaa atacgccgtc ttttagaaca
    28921 tcgtgatacc cttttgtcgg aatttttact tcgtttcccg ttatcgtgcg tttttgaagc
    28981 gtttccttta ccgtgaacaa gaaaataacg aaagagataa aatacccgat cgatgccgaa
    29041 taaaaaatca gagagtaaga tttaatggcc aatatcccgc cgaaaatcgg accgatggca
    29101 atgccggcat tattgaccat tcgcatcaga gaataaactt ccaaacggtc ttcggctttc
    29161 accatgtctg cgatcatgga attggacccg atggggtaaa acaaatccat cgctccccaa
    29221 atgcccatga ggatcgcaaa ttgccaaatc gtctgggcat ataccatcaa aaaataatag
    29281 acggttcctc ccaaaagaga agccagcatc acaccccgcc gtccgaactt atcacagatt
    29341 gtgcccgcga caaaagatgc cagaatcccc gtcaccgctc ttaaagaaat caatagggtc
    29401 gttgtccgaa gtggcagatt taaccgttcg cggaggtaga tgttcaggaa aggccaagcc
    29461 atggaagacc cgatggaaaa aattaacgat ccggcaaaaa tcacccagat ttggagcgga
    29521 atccgttgaa aattttgttt cagttttaaa ggaaaatgca tcggacctca gcgaccgtaa
    29581 aattgatcag cttgacaagt ttatcatagc atcgtgggtt tcatcctgaa cattcgtaca
    29641 acggaagaaa ttgaaggctg tttatgatag aatttgagct tagaatctca gtcgggaagg
    29701 aagaaacgct gagaagtcga attcaagagg taataaaatg aaacaacccg tacttgtcac
    29761 agcagcctgg ccctatgcaa acgctttcat tcatgtcggc aatctttcag gtgcttatct
    29821 tcctgccgat atctttgcgc gatatcagcg attgaaaggc agagacaccc tttttgtatc
    29881 cggcacggat gcacacggga caccgattac cgttcgagcg gatgacgaaa aaacaacccc
    29941 cgaagtcatc tataagcgat atcacgaatc atttttggat atgcagcaaa aagtcggcat
    30001 ttcatatgat ctttttactt ccacacatac ggaaaaccat tttaaagtct ctcaggatat
    30061 ctttctcgcc ttgcaagaga acggctattt gtataaagcc accgaaaagc aatggtatgc
    30121 ccccagtcag gaaaagtttc tgccggaccg atatgtcgaa ggaacttgct atatctgcgg
    30181 gtatgataac gcgcgaggtg accaatgtga taaatgcggg aatctgctgg atccggaaaa
    30241 attaattaat ccgcgttcga aaatagacgg ttccacaccg gagttacgag agacggagca
    30301 cttttatctc gatctcggaa aactccaaaa tcgggtggtt gattttttga aatcacgcga
    30361 ggattattgg cgccccaatg ttattcgaca gtcgctgggg cagattctcg ccgaagattt
    30421 acacggccgg gcaatcaccc gtgatctgga atggggaatt ccggttccgg ttcaagatgc
    30481 cgtgggaaag tgtttatacg tctggtttga agcggtcatc ggttatttat ccgcttcaat
    30541 cgaatgggcg aaattgcagg gtacaccgga catctggaaa gattggtggg aagatgatac
    30601 ctgtaaatca tactatttca tcggcaagga taatatcccc tttcatgctg ttatctggcc
    30661 ggcacagtta atcggtaccg gaactaaatt cggcgtatat ttcgaaggaa aggaaaacaa
    30721 accgtttgtt cttccctttg atgtccccgc aaacgaattt atgaacttgg aagggaaaaa
    30781 gatctccggc agtcgaaatt gggcgattta cgtcaacgat tttctggaac ggtatgatcc
    30841 ggatccgttg cgatattatt tgacagttaa catgcccgaa tctcaggata ccgattggga
    30901 ttgggaagac tttttgaaac gaaataacaa tgaattggtt gcgacttggg gtaacctggc
    30961 aaatcgggtc atttcttttg ccaacaagca ttgggggcag gttcccgatc cgggaacatt
    31021 ggaaatggaa gatcaagatc tcattgatac gatcgaaagc ggctttttaa ccgtcggtga
    31081 tgaatatgaa gcggttcact tgcgagctgc cttggcggag accatgcgct tggcaacgga
    31141 agtcaatcgc tatctcgata tcgccgctcc ttggcaactg atcaaaacgg acaaagaagc
    31201 agccggccgg aaaatatata cggctctgca ggctattaat aatctcaaag tgcttttttc
    31261 gcccgttttg ccatttactt ctcagaaatt acacaatatg ctcggctatt cgtcgaatct
    31321 gtttggtgaa caagtgatca agacggtaga agataatctc ggtgagcacc gggtaaacac
    31381 ctacgacgct tccgaggcga tcggttcttg gcaaccggcc gaattgccgg tcggtcaaaa
    31441 attcggtgag gtttgtccgc tgtttaagaa attggacaca tcgatcgtcg atgaggaaag
    31501 agcccggtta gggtaatcat tttcatcata aattgtttac aaaaagggct cttctttcgg
    31561 tgagcccttg tattttaaaa ttctatatca cgcggacgga ggattgtata actccggata
    31621 aggatcttcg tttcgaattt tacaagtcac gaaatgcaaa gtcactttca gcagcccgag
    31681 aataggcgcc gccaacaaag cgccgaataa acctattttt ttatttaaaa tcaatatacc
    31741 gataatgatc agaaccggat ggatatccaa ggcatggctc attactcgcg gaacgacgat
    31801 gatgttttct atctgttgaa tcagaatata agcgataatc acgaccaaca ccatcagaaa
    31861 attatccagc ggcagccaat aagatccgaa aatcgcagct gaaaaaattg ccggaataca
    31921 ggagataatg ggaccgatat tcggaatgac ttccaaaaat ccggctacga tccctaacgg
    31981 caaagcccaa tgtagcccca atgccgtgca tacaccgaaa gtcacgatac cgataatcag
    32041 catcaatttc agttcaccca caaaatagct gttccaaata tttcctaacc gattaaggaa
    32101 ttcatcggtt tcgatctgat aattctccgg aaattgctta acgatccagt ctttcagttt
    32161 ccaaccgtcc ctgcataaat ggatggaaat catgatagaa gtaacgatgg tcaaaagccc
    32221 gccgagaatt ctggagatta aatttgcgga aacagatatg atagactgcc aagccgaaga
    32281 tatgtccgga atgatcgatg aaatattaaa atcttccaca ttaatggaat tcagctttcc
    32341 gcgcaactcg atcaacggga cggataaatc cactccgaca ttcccgatct gtattttttg
    32401 gacttccaat tgccgaatca ttccttccaa ccaggaatcg atactgttga taaccgtcgg
    32461 ccagtcggta gaaaggaacc cttgaatccg gccggtgata accggtacaa tcagaaccaa
    32521 taaaatgatg ataagaataa tcagaatcag gtatgccagt acgaccgcaa accctcttcc
    32581 catgtgcaat tttcggacaa agaaatttac caccggcgac aggatatacg caacaatcgc
    32641 caccgcaata atcagattca aagtttcatg ggaaagataa acgacggcgc ccgcaaacag
    32701 aatcaagccg accaaagcca tatatcgaat cggattactc caccggtgat ttttttcttc
    32761 catcttttct tccgtcattt attaaaaaag ttattcgggt tttgaggaat gccgttatat
    32821 cgcatctcaa aatgcaaatg cggtccggat gagttaccgg ttgttcctac ggcaccgatt
    32881 acatcccctg cggccacata gtaaccgcat tgtacattat aagaatctaa atgtgcatac
    32941 aaagtctgcc atccgtttcc atgatcgatc ataacaacat ttccgtatcc ccaatcattc
    33001 caaccggcat ataccaccac gcccgcatcg gaagcataaa ccgcgttacc ggtcgaaccg
    33061 gcaatatcga ttgcccgatg attggtttct tcggaatagt tatatcccga tatgcgggtt
    33121 tcagtcgtcg gccagataaa tacaccgctg ccgaccggtc cgtccatgac agactgacag
    33181 gctcccggtc catagatgga agctaccgcc ggattttccc gagtaataaa cggagcggac
    33241 cagtttgtga attcacggga tcctccgggt acgatcagcc atgttccggg cgcgatattg
    33301 ggatgactgt aatccccgat agttgccgga tccaacccgt tcccagggta attgataata
    33361 tcttccggcg tcactccgta aacttcggcg acgccattca gcccgtcacc gtcatgccaa
    33421 tcataataaa ccccgtccac cggtaggata tttaattcca tcccttctac aaccaaatgc
    33481 gggttgtcag ccagcgtata aggattcgcc cataaaattg tactgggacg taaattgaat
    33541 ttttcggcga tcgacgttac ggtatctcct ttttgaacga catatttaat gacatctgtc
    33601 cgcgcttttg cgggtaattc ggtgtcattc ttggtactgc gaaaaacacc ttcatttaaa
    33661 ggctttaact cgcctttcgg ataagccgga tcataataag gcaaaaccgc gctctgtcgg
    33721 accggagtag accgcagcgt ttcctgaaga ttgatcgcat cggtctgcgt atcctccatg
    33781 aaaaagtttc cggtcaccca aagtacagtc ccgattaaca gaaccgtaac caccgcagag
    33841 cctattttga gaagcctttc tcccaacccg gcttgaataa ttttcagcca taaatcggaa
    33901 attgatttct tgcgtttatt ttgttgcccc attttctttt ggacaacccc atttgatccg
    33961 tcttttgatt ccatatttta ttcaattttt agatcataac atatttctgt ttcggataaa
    34021 ataataattt cttaaaaaat gtttgcggag ccgcaaagat tacaagaatc ttaatttttg
    34081 attcgaaaat gaataaatca tctgttcgac gctataataa aagtcctatg acagcaaata
    34141 tttatccttt tacagcaatt gtcgggcagg aacaaatggt tcgtgccctg atactgaatg
    34201 ccattgatcc gcgaatcggc ggcgttttaa ttcgcggtga acgcggcaca gccaaatcca
    34261 ccgccgccag aggcttggcc gccgttttac cggaagaaga agtgatcgcc gaatgtcgtt
    34321 tcggctgtga tccggatgat gagttacatt ggtgcacgga atgcaaagaa cgaaaagcaa
    34381 aagggattcc gatgacacga actcgccgga agacaccatt cattaattta ccggtgtccg
    34441 caaccgaaga ccgcgtagtc ggcacgttgg atatcgaaaa agcattaaaa accggcgaaa
    34501 agagtttcga acccggtata ttggcttccg ccaatcgcgg gattttgtat atcgatgaag
    34561 tcaatctcct cgaagatcat gtcgtggaca tcctgctcga ttccgccgcg atgggtgtaa
    34621 acagcatcga acgggaaggt atctcttttt cgcatcccgc tcgatttatc cttgtcggaa
    34681 ccatgaatcc cgaagaaggg gatttgcgtc cgcaactttt agatcgtttc gcactttccg
    34741 ttgatatccg cggcattgaa gatgctcggg agcgtgtcga aatcatgcgc agaacattac
    34801 gattcgaaga ttcgcccgaa ctttttcagg cggaatggca aaaaagtgaa gcggaactga
    34861 cagaaaaaat cgcatccgct cgaaaacgtt tggcttcggt ccgttacact tctcgcgatc
    34921 tgatcacaat cgcttcgctt acctcttcat taaaagtgga aggacatcgt gcggatttgg
    34981 tgattttgaa agccgcccga gcacaggctg cttttgaaaa ccatccttcc gttacggatc
    35041 tggatattgc gatggctgcg gaattggctt tacctcaccg cctgcaaaaa ggtccgttca
    35101 gtgaaagcac cgtcggtacg gatgaattaa aacagcgaat cgacgaatta cacggtaaaa
    35161 cctccgacag tacgtcattc caatccgaga gcgatgcggg tgacgaggaa aaaaaaattt
    35221 aacttcctcc gatctgcaaa atttaatccc tattcaggtg gcacaatatt cacaaggggg
    35281 ggattcgatt tttaacaacg cggaagccaa ttggtgggac aacgcgcaaa atctcttctt
    35341 tatcaatccg gaattactcg acgataacaa acccaatctt tcgcaaaaaa agagcagcgg
    35401 aaaacgaagt catattattt ccaaagatcg ccgcggaaat tattataaag ctcggattgc
    35461 cgaggatgcg gtaaatgatc tggcgttgga tgcaacgtta cgtgcagcgg ctccttatca
    35521 aaaatcgcgc aggaccaatc cttccgaccg ttcgatcctg attaaacatc aggatctccg
    35581 ccgaaaagtc cgttcccaac gggcgtccgg cttactgctt tttctgctgg atttatcttg
    35641 gtccatggca gtccggcaac ggatgaatgc cacccgcgga gcaatccttt ctcttttgac
    35701 ggatgcatat caacaccggg atcgggtcgg tttgatcact tttcaaaaag actccgccga
    35761 attggtgctt ccgccgacct attccgttac cttggcagaa agatcgatga aaaaggttca
    35821 ggtgggcggg aaaacaccgt tgacagccgg gttatggaaa gcttacgaag tcttggaacg
    35881 ggaaagaaaa cgccatcccg atgagaatgc actgttgatt gttttgaccg acggtgtcgg
    35941 aaacatttcg atgtttgata aggctccgga agaagaatcc aaaatcatcg ccgaaaaaat
    36001 cgcggcggaa aacttccgca gcatcgttat tgatttggat actcaagact ttgatcaggg
    36061 aatgaccaga aaactcgctg ctcagctcaa agctccctgt tatctgattt ccgaattgaa
    36121 agcggatcgt ctggcaaaaa ttatccgaaa agagatctaa agatcgccga ttggttctcc
    36181 gactgcgaac gggaacttat cgacgttcga aataaagcat ccgagatgaa agaacccaaa
    36241 tcaaatcacg ttattcggat ccgaaattta ggctgagatc ggcggaaagc gttaaaaaaa
    36301 gaaattgacg gttataatag cttttgaata aatcctgtcg aagaaatctg gaaatattta
    36361 tggatgaaaa gacatcactg aaagagtttt taaaaagcat tatttcactt cccggaattt
    36421 cgggatatga aacgacggtc agctcggcaa tagccgagaa atggagaccg ttaaccgacg
    36481 aaataattgt ttcaccggtc ggaaatctgt atgggattaa gaagggattc ctttcaaaaa
    36541 gcgggaaacc gtcgaaacga atcatgatgg ccgtgcatat ggacggaatc gggatggtaa
    36601 tcgaacgaat tgaaaaagat gttctttact ttgccccgat aggcggattc gatccgcgaa
    36661 ttcttccggg acaagaagta attattcaca gccgatcggg tgatatccgg ggaataatcg
    36721 ttcaactggc gccgcatctt ctttccgagc cgtttaccgg taaaccggtc ccgttgaatc
    36781 aacttgtgat cgataccggg ttgaacgcgg aagaactgaa agagaccgtt caggcggggg
    36841 atacggtatc ctatgccaat ttaccttttg aaatgccgag cgattgcatc gccgggcata
    36901 gtttagacaa ccgcgcctcc gttgcggcag ttacgcaatg tttaaccgag ttgcaacgtt
    36961 ttaaccatgc ttgggatgtt tatgccaccg gtaccactca agaagaaacc gtatttctca
    37021 gtcgaacgac cgtgagtgat gtgaaaccgg acatcgcaat tgcgattgat gtaacctttg
    37081 ccaaaggacc cggtgtggat aactacaaag gaaaacctct ggagagcggc ccctccatcg
    37141 gattcggagc caatattcat ccggtattat tcaaccagct gaaacaacta tgcgaacaat
    37201 tggacatccc gtatacattg gatccgctcc cgcaaatgtc tgggacggat gcgcacccga
    37261 tccaaaccgc ttcgggcgga gtggcaacct gtcttttgag tatcccgctc cgatatatgc
    37321 acacaccggt cgagatgatc tctatcaaag atgtgatgcg cgccggtcat ctgatggcgg
    37381 aatacattgc cagattggat gaaaacagtt tggattccat aaaatgggag gattaaacct
    37441 atgataaaag aagaaaaatc aacaatcgat tccctccctg ttttgccgga aagcagcgta
    37501 cggctccttg aagaactgag taacgcggta ggagtttccg gaaacgagaa tgaaattcgc
    37561 tcgattatcc gtcgggaagt aagctccgtc gcagacaatc tgacgacgga tacgatcggt
    37621 aatttgattg ccgtcaaaca tgcaaaaaac gatcacgcac tgcgggtaat ggtcgccgcg
    37681 catatggatg aagtcggatt catgatcgtc gataaagaat ccgacgggat attcaagttt
    37741 caggtcgtag gcggtatcga cgaacgtgcg ctggccggaa aagcggttca ggtcggcaaa
    37801 aagcacgccc ccggagttat cggcgcctgt cccgttcatt tatcgacgcc cgccgaacgg
    37861 gaagtgattt tgaagaccga caatctccga atcgataccg gtccggggtc caaagacatc
    37921 gaagtgggcg attatgcgac ttttgcaaca aaattcaagc gtatgggcgg cagtttctgc
    37981 gggaaagcaa tcgataaccg catcggggtg gcaacgttga tcgaattatt gaaaaacgcc
    38041 ccggattcaa tcgaactcat tgcggctttc acggtacagg aagaagtcgg tttgagaggg
    38101 gcggaagttg ccacttataa tatcaatccc gattttgcct ttattatcga ctgcacaccc
    38161 gccaacgatc aacccgcctg ggatcattcc gaaaatacgc tctatcggac aaagttggat
    38221 cagggtccgg caatctacac aaacgacagc agaacgctct atgatcatcg tttgattcgc
    38281 tatttgtcgg atttgggaga tcggtacgga attcgttatc aatatcgaca accggcaagc
    38341 ggagggacgg atgccggcgc catccataca agtcgtagcg gaatcccgac gatttcgctt
    38401 tccgttcccg gtcgttatac ccataccgcc ggttccatta tccggaaggc cgattgggaa
    38461 gatcactatc gattaatgac cgttgcactg cgtcatatcg atgcggatat tttaaaagaa
    38521 aatcgctgaa gcacagcttt ttttgaccat cttggagaat ctatgaaaaa tttgcttgaa
    38581 aaactgacac aaacaaccgg tgtttccgga tatgaaaatg aaattcggga agttattcga
    38641 aaagaaatcg aaccttttgc ggatgacatc caaatcgacg ttatgggaaa cctgatcgcc
    38701 cgcaaaggaa tcaagaaaag tgggggcaaa cggatcatga tttcatcgca tatggatgaa
    38761 atcggtctga tggcttccca tatcaacgat aaaggttttg tttacgtcgc cacaatcgga
    38821 ggagttaatc cgctgacctg cttcagtgcc agagtaaagt ttctaaacgg tacggaaggc
    38881 gttgtgtatg cggagaccca aaaaatggat aaagtcccga ccgttgatca gctttttgtc
    38941 gatgtcggcg ccgattcggc cgaaacctgt ccggtaaaag tcggagatga agctgttttt
    39001 gtccgatcgt ttttggatat gggaaatcgg gttgtttcca aagcgattga tgatcggatc
    39061 ggctgtctgg tcgcaatcga aacgatcaaa gccctcaaag attcgccgaa tgagctcttt
    39121 tttgtctttt ccacccaaga ggaagtcggt ttaagaggtg caaaaacatc ggcattctca
    39181 atcaatcccg agatgggaat cgccgttgat gtcactctcg cagccgatgt tcctcattgc
    39241 gagccgggaa atccggtttt aggcaaaggt ccctgcatta aagtccgaga cggttctctg
    39301 atcgctcatc cgaaggtaat cgacgcgatg aaaaaagcgg cggatcgaac cggtatcgaa
    39361 gtacaaatgg aagttttgag ccatgccgga accgatgccg gggcaattca gttggttcgt
    39421 tcgggagttc cggccggttg tttgtcgatt gcctgccgtt acgttcactc gccttccgaa
    39481 atggtcgata tcaacgatgt gagtgaatct gtccgtcttc tggtcgatct cctgtcgaat
    39541 ccggttgatt tataaaagga ttatccgtgc gggagtaaaa taaatcagag agcaaatggc
    39601 aagaatacac ttttttaaaa aacatcctat tcgcttcggt gcagctgttt tggtatctgc
    39661 tttattggtt attctgataa tttccggttg ttctgaagta aaaaaaatgt tagccggtac
    39721 tccgacggcg acgataatgc ccgctgtgaa acagactgcg acaccggtat caaacgaaga
    39781 agaaattatt ctgcccgaga atgaatcgac ttcggagtcg tcgcccaccg ttgacgattc
    39841 cgatgagact tccgtcccga atcgttcaaa ttcggaaaac cggactctca ccctttgggt
    39901 acccccgcaa tttgatcctt catccaatga tgaagccggt aaaattttga cagaaatgct
    39961 caaaattttc atggaagaaa atcccaacac gctcatcaat attcgagtca aatcgctgac
    40021 cggcgaaggc agtgccatca acaccttatc cgcggctgta aatgcagctc ccgaagtgat
    40081 cccttctctg attatcatga atcgtaatga tttggtgtca gccgttcaaa aaggattgat
    40141 ttatccgatc agcaccggtt tatttaccga tcctgcttca tggtaccctt tcgcaaaaga
    40201 gtcatccaca gtcaataatt tgatttatgg gatcccgatt gccggcgatc ctttggtctt
    40261 gggttatcgt ccgtcgaaaa cgggatcgga aataaatacc tgggatgaga ttctgtcccg
    40321 aggattgccg atcgcttttg aaccttccaa cccgtatgat ttattcggaa ccttcattta
    40381 tctggcaaag ggcgggaaaa ttacaaacga tatggggcaa ccctggctcg atcaagccaa
    40441 gttgaccgac acgctcaact ttttcctttc cggagggcag aaaggggctt tccctccttc
    40501 tttggcgcag ggaacaagcg gatcgggcgc caacgaaaat tggcaattgt ttttggaagg
    40561 atctttacat attatcgtca ccaaactgac caccttccgg cacagccaaa atgccgatat
    40621 cgatgccatc ccgcttccgt tatttaacgg aagcagcagc tatcctctgg taaatacatg
    40681 gaatgtggta ctcagcaaca gcaatccgga aattcagaat ctggcggtaa aactggctga
    40741 aaaatttgcg gatccgcgct ttaatgatca gtggactcaa tccgccggat atcttcctgt
    40801 ccgctctatc ggacagactc tatggcaaaa tgacagtttt tacgagaaaa tgatcacgat
    40861 ttgtgaaaac ggagaattga ttcaaaccga ccaaattttg aataaaatca cgcccgtcat
    40921 caacgaagca gtatcctccg tcattaaaac cggagcaact cccgaagatg ccgccgaaaa
    40981 agcaatcgat gcaatcaatt aagtaagttg ggatgaacat gtcaaaaaac tcgaaatcgc
    41041 agcgaagaag atttaattgg atccttttcc ttatcatttg tattttggtg gtttttgctt
    41101 tcatcctctc ggaatcggat aaaattgaac atgcttccca aacatcgcga acgattcaaa
    41161 tgggtttatt gcaaaccgcc gaaccgacaa tcattttacc gactccgact ccattcccct
    41221 cggattatcc gaagcttccg gctgaatatt ttgaaaacga agatcagaca gacggcgtaa
    41281 tcttaggcgg gacggttctg gtcttgatta ttctcatcgg aacaatggtc ttcatccaaa
    41341 aagaacatca aaaaaagaat gagaaataaa aatcgtcctc ttttttatga gaataaaaaa
    41401 aagacctggc cctatgtcgt ttttatcctg ataaccatcg ttatcctgat catctgggga
    41461 gctcaggtgc ccgttattcg acaaaccttg ggacgatctg ttttggatgg catctcttac
    41521 ggaaaaagcc tgattttgcc tgtcagtacg gttaaccccc ttaattatcg cattcaaacc
    41581 tattatccga cccttgatct gccgtcgaaa gaagtgattt tcgccgagaa tctggaagat
    41641 atcaattcat ccgaacctta tcttgaaatt acctttacac ccacaccgac accgggtgtt
    41701 ttatgggtca tcgaagatcc tgagaaatca agtaccaatg attcggcgac actttccgag
    41761 gaagtgaatg atcgactcga aatgccgata tttgaaatgg cggattacct gaatgacgga
    41821 ccggcttctc tcagcacatt cttgagattt tatgagaaac cggaaagtca gtatttggtg
    41881 agtgaaaaaa taaagccgga ttattttgat cccaatgtat ctctcaatga aatgctgtct
    41941 tacagcgagc aaaaatatcc cgatctttcc ggtatcatac ggaaaaacgg gaacatccaa
    42001 attctggctt ctttaatcga tagccatttt ccggtgctga ttcgactgca gtaccaacga
    42061 cagcaatccg cctggaaagg cgatgacctt tgggacgctc gttatgtcgt gatttccggg
    42121 atcgattcaa aaaacaaaat cgtttatttt tccgaccctt taaaagggaa cgaacaaacg
    42181 ctctcttttg atcgattgat ggaagactgg tatccgttcc gtagagaata tctggttatc
    42241 tatcccgtcg atcgcgaaga atccctcgct ctattgttga attcggactg ggatgaagag
    42301 gaaaattcaa aaaaggctct ggagaaattt cagacagatg tgaccatggt tccggataat
    42361 caatttgcct ggttcaatgc cgctgctttg ctgattgaga ataatcacaa tgaagaagcg
    42421 tgggattctt ttcgtaccgc tttacaagtc ggcattcctc agcgttatct tttatatgat
    42481 ttttccctgt acgaggcggc ttttaagaac ggattggctg aagaattacg tgatcgcaca
    42541 gctgccatgc taaaaatcaa caatcattcg gaagaaaatt ggttatggaa cggttgggct
    42601 tatactttat tacaagatag aagcgaagcg gaaaaatgct ttcaaaaagt cttggatatt
    42661 aatccgaaca acagcgacgg acaatatgcg atggaatacc tcaaacagta tgaataaaga
    42721 aatgggaagg actcaaaatg gaaaagaaga ataacaataa gacgataata attgttgtca
    42781 ttaccgtcat cgtattaatc ggttgtctct gtctgtcagc cgctgctctc ggagtctatt
    42841 tcctcaatca ccggatcgac aaaaataatg aagaacttat ttatgaaacc gccgttcccg
    42901 agacttcacc cgaagatcaa accgcacttc ccgaagttac ccctgacaac gaaacagacg
    42961 gaaccgaaat caataatacg gaaattaccc aaaaacaaca ggaaattatc aaaagagccg
    43021 aaaagatacg gggattaaag gcacaaggtg atttcaatat tacttttctg acaaaagaag
    43081 aattacgaga ccaacttatt aatgacatgt atcaggatac gacgaagcag gactttcaag
    43141 acgagcatga tattttaacg cttctcggtt ttattcccgc agaaatggat cttgaatcat
    43201 tttacctgga cttttatacg gaacaaatcg cgggatatta tgacgaagat gcaaacgtaa
    43261 tgtacttaat caaaggagga tccgaaaccg ataactcatt gactttagcc catgaatata
    43321 ctcacttcct gcaatttgat cattttaatc ctgcgaaaac tctgaattac aacgatgagt
    43381 attgtgaaaa aaatcaggaa tattgttttg tgttgggatc tgtttttgaa ggggacgcaa
    43441 cattgacgga gatgctttta gccgcacaac ctgatttggg gttatatgat gagcgcatgg
    43501 ttgaacagga tacgtccgtt tttgataatg cgccgcaata tttccaaaat tatcttcttt
    43561 tcccttatac ctatggattt gattttgttt cttcggttta tcgaaagggc ggtttttcgg
    43621 ctgtgaatga tttgtattcg aatccgcctc tcagcgccga acaaatcatg catcccgaaa
    43681 aatattccgc cgaccagccg gttaatgttc tgttcgaacc ctttcaaaag aatctcgatg
    43741 aaagttgtga tttggtctat gacaacgtct tgaatgaagc ggatttatta tggttgctga
    43801 acagcggcta tgaaaaaagt tggcgaattt ccgataaatc cgctaaaaaa gccgcggcag
    43861 gatggggtgg cggagctttc caattcgcac gatgtgacgg caaaccgatg tttttcggga
    43921 aaaccgtctg ggataccgtc aacgatgcca aggaattttc tcagagtctg aaagattaca
    43981 acgacaagcg ctggggaaaa agtaaaataa acggatcttg gaccggaact gacggagaac
    44041 aaatttatgt ttccgttcag gatgacaagg tgttttatat gatcgctccg gccgaatttt
    44101 caccggattc attgatcgac ctgattgaaa ccggtcaggg attataaaac ggaaaaagcg
    44161 gaaaatattt ccgtttcatc cataatccga atcaaataat caacatttcg agaagaaaag
    44221 aattcggttt actccgaatt ctttttgaga tattccatcc attctttgat tttcttttca
    44281 tagccttgtt cgccgggatg ataaaaatcc gttttcaccc cgtcggggag gtattgttgt
    44341 ttcacataat gattgggaaa ctcgtgcgga tataaatatc cttgcccgtg tcccaaccct
    44401 ttcgcatctc ggctggcatc cattaacggt aaaggaacct gcccggtgcc ttgctcattt
    44461 atatgatcca tcgccttgaa aatcgcgccc accgaattgg atttcggtgc agtcgccaaa
    44521 tagagaattg cctctccgat cggatagtat ccttccggca taccgatata gtcaaaagca
    44581 gtagccgccg ccactgccac ctgcagcgca ttgggatcag ccagtccgat atcttcgctg
    44641 gctaacacaa tcaatctccg taaaataaaa tgcggatctt ctcccgccaa caacattttt
    44701 gccgcccaat acaaagccgc gtcggggtcg ctgccgcgaa cggatttgat aaatgccgaa
    44761 atggtatcgt agtgcatatc gccgttttta tcatattgaa gcgccctttt ttgtatggat
    44821 tcttcggcga ttttgagcgt tatatgaatg atgccgtttt catccggctt ggtgctttca
    44881 accgctaatt ccaaaccgtt caaaagattc cgcgcatccc ctccggccaa atccaacaac
    44941 aaccggcgtg cgtcatcatc caatttcaca ttcagctttc cgtatcctct ctccggatcc
    45001 gataaagccc gattcaaaat aatgttgaga tcggtttcat tcaacggttt caactcaaaa
    45061 acacgcgaac gcgacagtaa tgctttaata acctcaaaat aggggttttc ggtggtcgcg
    45121 ccgatcaaag tgatcaaccc gttttcgaca tgcggcagca gagcatcctg ctgtgcctta
    45181 ttccaacgat gaacttcgtc cacaaaaagg agtgtccggg tccgatacaa tttcctccgt
    45241 tcctgtgcgt caacaatcac tttacgaagg tcggcgactc ctgccaagac agccgaaacc
    45301 gattcaaaat aagctttcga ctgagcggcg atgatttgcg ccaatgtggt tttccccgtc
    45361 cccgggggac cgaacaagat gatggaggaa aagagtttgt cggattcgat tgcccttcga
    45421 agtaattttc cttctcccag aatatgttcc tgtccgacaa actcatctaa actccgcggg
    45481 cgcatccgtg ccgccagcgg ggttgtttgc atcatgtttt cccgatccgt atagtcaaaa
    45541 aggtccatat ctcaaaaaaa ttatactcta cggctaaaaa ttccgcggat gaaaagcgga
    45601 tcaacatatt caaggtattg aaggtatact attaataatc aaatattctt tatttgattt
    45661 tacaaagggc tgagcaacct attttaaatc gataaaaagg agaaagaagg agaaatgaat
    45721 aaaaaaatct tatcagtttt cttggttcta tcagttctgc tcaccgttgt tttttcggcc
    45781 ggtgcgcagg ataccgccgc ggagaccgta aaattaacca ttttaggcac aaatgatatc
    45841 cacagctata cggcagacac accggacaat gtcgccgagg gggaaacagc taagaccgga
    45901 tccatcggat atgctaaaat tgccgcttat aaaaaacagg ttgcccaaaa cggtccggta
    45961 ttgctttttg acagcggtga tgccacccat gggcaggtta ttgccactct cttggaagga
    46021 gaatcgatca tttggttgat gaatgaagcc ggttatgacg cgatgactcc cggaaatcat
    46081 gatttcaact acgggtatca aaaattgatt gatttcaact acatggcgga tttcccgatc
    46141 ctttccggga atctggttta tgccgattca caaatgagtg ttcttcccga atacacgatt
    46201 tttgatgtcg acggaatcaa agtgggtgtc tttggaattt ccacccccga aacattatat
    46261 aaatcctcac cggtgaacac cgaaggtctg caatttatcg atccttatga agcgggaaaa
    46321 tattatgcgg attttctgcg tcctcatgtc gatcttgtcg tttgcctgtc tcatttgggg
    46381 cttgatgccg gcagtgagtt taccagtgaa ggcttggcaa atgcggttcc cggaatcgat
    46441 gtcataatcg atgcccacag tcacacggaa ttaccggagg ggaaattggt ggggaatacc
    46501 ttaatcgctc aagcaggcga acacggacgc tttatcgatc aggttgaaat cgaactggca
    46561 gccggacaag cttcgaccga ccggatgacc gttgtgtcaa aatccgcaaa actcatcacc
    46621 tacggagatc tcttaaatct tgaaccggat ccggatgtcc ttgaaatcaa taccgagatc
    46681 gaaaaacaga ttaatgagat ttcatccgta gtgatcggaa aaaccacggt ggatttggac
    46741 ggcgaacggg aaacaaaccg gaaacaagaa accaacttgg gagatctgat cgctcaggca
    46801 atgttgcttg aaaccggagc ggacgttgcg attaccaacg gcggaggaat acgagcatcg
    46861 attcccgccg gcgatgtgac gttaggtcag gtaatcacca cattcccgtt cggcaactat
    46921 gttgtaacga ttgaagccac cggacaggat ttgatcgatg ccatggaaaa cggtctgagt
    46981 gattatccgg agctgaaagg cgcattccct cagattgcgg gaatgcgggt tgtattcgat
    47041 ccttccaaac cggccggcag tcgtgtcgtc gaactcacgg tggatggaaa accggtcgat
    47101 ccggcggcga cttatgtggt tgctaccaac aattttatgg cgatcggcgg ggatgaatat
    47161 accatgtttg cggatgataa attgctcggt gaatattccg cttttgatga aatcctggcg
    47221 gattatctca caacatacgg caccgagggg cttgaagttg acggaagaat ctctacggtt
    47281 caataaacgg aacagagccc gattttaaat gatgttgtta tagggacaaa aagaaaatcg
    47341 gggcaggatc tttttcccgg ggcgggcagt tgtgactgtt cgcccttttt tttagacata
    47401 tgggataagg tgcggtttag aaacgtccta tcttctttcc gtaaacatga tatctattta
    47461 cggaacggtt cgatcggtat gaagccaaaa tagtaggaag aaaaccaaaa aagttgagac
    47521 accggggttt ccggaggcgg agaaaaaaac aatgataaaa tattctcaaa tccccattga
    47581 aaagaggcca ctgtcatgct ccgtattgca atctgcgacg atatggcaga cgaactgcaa
    47641 atccttctct cgttgacaaa tcagtacatc tcaactaacc atcttgatgt cgatgtaaag
    47701 gagtttactc atcccgatca attactcacc gctatcatca tagaaagctt tcatctttat
    47761 atcttagacg ttgtcatgcc gatgataagc gggttggaat tgggtaaaga aatacgccgc
    47821 ttggatcagg aggcacaaat tatctttgtt actacggagc cgcaatttgc cttacaagcc
    47881 tatgcagcga atcctataaa ctatcttatt aaaccaatcc aaaaacaaca gttttttgat
    47941 actttagcac tggttgtctc taaagcagac tttgcggacg agcaaacatt tgccgttaaa
    48001 accgccgaag gactgcgggt tatcagactg cgggacatca tctgctgcga atatcgctgt
    48061 cacgctgtta tcttcagttt gacaaacggt gaagaaatac tcagccggac atttcgagag
    48121 aatttctcaa attacgcctc aacaattctg aaggacaggc attttttaca atgtcactcc
    48181 tcatttgtaa tcaatctgcg ccgggtggag cgtttcacaa aggagaactt cacattgacc
    48241 ggcgggaaaa tgatacccat cgcggcgaag caatatccta cagttcgaga tacatatatg
    48301 gactatctga caggaatagg aaaccgttaa tgaaagaact ttttcccgac ttggtgcggg
    48361 cggcaatctc aactaccgcc aacgtccttc tgatgatgac gcttttacag ccgagatatt
    48421 ccaaaaagat tacactgctg actatgttcg gcattttagc cgccgatttg gggacagcaa
    48481 tttattgtta cctcagcaga aatttaaccc tgctttctaa gctggatgtt attttgtttg
    48541 cggttctatg ctttgcggtt cggccactat tcaaggacac cttcatgcag tggctgtttt
    48601 cctatctaac cgtacagaac atcagcgatg ttgtgattat cctcagtttt atcggctcaa
    48661 gacatcttcc ttatccggtt tatgccaact cgctgctgcg tttcatcctg ttcggtgttt
    48721 tcctgttgat tttatcccgc cgggtacgcc cgctgtaccg gcaggcggtt gagcattgga
    48781 cagcatactt tgctgtggca ttaagcattt acctcacgtt caattactat gttttgtctt
    48841 cggatgatat tgttgttact ctgacggagc aagcaatacc gcttctttta gtgattttta
    48901 tcagccttgc cgcatatgga tctatttttc tctccctaaa aaaccttcag caagagtttc
    48961 aaattaaaga ggagaatcgg aaaatgcagg cggaacgaga ttacctgcaa ttagcggccc
    49021 gaaatatgtc tcgacgcttg gaattgatgg cggaggtatc ggaacaaaat agccgtgccg
    49081 cccacgaccg tcatcacttt aataatgtgc ttctggagct tctggaggag ggaaaaactg
    49141 accaggctca agctttattg caaagtcaaa atcaagtcgc tcatcaaatc agcaaggtct
    49201 attgtgaaaa ttcagctgtc aacgctgccg tctgccatta tacggatctt gcggagcagg
    49261 agggtataaa aaccgagatt atgctggata ttccgagcaa cctgagtgtg gatgcattgg
    49321 aattttcgat ggtggtttca aatctgatgg aaaatgcggt tcaggcttgt aaaagactgc
    49381 cggaagaaca agttccctat ctccgtttca cctgccgata cgtagggcga ctgttactgg
    49441 aaatggaaaa cccctgtgca aataattcca tgctggacga atgcggttat ccggtcaccc
    49501 ccgaggaaaa tcacggaatc ggcagcaaga gtgtcattgc ttttgcaaaa aaatatgacg
    49561 gcgagctgat ctacaaaatc gaaaacagta tttttcaggt tcgacttctg atatgagatt
    49621 acgggaaatt cggctggcaa gagatgattc gaggaataca agttattaag tgaaaaaatg
    49681 agccttttaa gtgcagagac tcattttttc cctccctggc attattctga aagcagagat
    49741 ttataaataa ttttgataaa acggaggaaa caaacatgaa aaagagaatt cttgcatcac
    49801 tgctgatgtc tttcttgctg gcttctttgg cgcttttcac taccgaattc tcgcagaata
    49861 tcggtgtatc aggcgcagct gcgccggagg atgccccagc cttatggaat gcgggcgaca
    49921 caaggaacaa gatcgtcgta ataagcgaca ttcacctcgg tattgatgac aactacaccg
    49981 aaacgctgca aaaccgcccg ctgctggttg attttttgcg gcgtttgcag agtactaacg
    50041 atgtccgaga attggtaatc gccggtgatt ttctggatga gtggtttttg ccggtctatt
    50101 atcccagcta tacagatgaa gaccgctttt atcaggatgt aattgtcaat aatcgggatg
    50161 ttatcgatga attgaacaac gtgatcgaca gcggaattaa actggtctat gtaccgggaa
    50221 accatgacat gacgctgaat gccggcattt tgcttgaagc gatccctgac attattcaag
    50281 cccgggattc caaaggactc ggtacttatt acaccggcga caggaatgag atcgctatcg
    50341 aacacgggca ccgatatgat gtgttttccg ctccggacac agtcaccaac agggaactat
    50401 gcggcaatga tgataccatt ttcccggccg gttattttta tgcccgctat gccgccacct
    50461 gggttttgga gggacggccg gccgtcaaaa aagatttacc ggtggtaacg gatattccgg
    50521 ataaaaccga ttcagatcaa tatggcgcat tcctttacta ttcgattctc aaagatatct
    50581 cggcacgaat gacacccaaa gagagtccgg aagaaaaaat atttgatatt catatcgctg
    50641 gatttgacga tgcctacagc tatctggatt tctatccggc acagcaatcg gacggaacca
    50701 tttcagcacc ggtgttgttt aaaaatattc agcgaacatg ggacgaacga caaacgctta
    50761 acaatgtaaa ggtgcctaac agctttattg aagcggtctc cggaaccata gattgggagt
    50821 attttttcag acaagcaaag gcacagtatc tggaaaatcc ggacgaaagc gtggatgtgg
    50881 tcatattcgg tcatactcat gtaccggttt ataaagattt ggggaacgga aaatattact
    50941 taaatgacgg aacttggatt gatcacaaca cagatttccc ggatgccacc cgtacctttg
    51001 cggtcattac taccggcaat aaggatcagg ttgcactgta taaatttgag gaagatggat
    51061 ctgtcaccga tataagcgcg agcgtcagtg agcaatgaaa caggttaatc tgcgcttacg
    51121 aaaatgcagt cttgttccga agatgaccct cccgggaaag tgaaacggtt tcgattgctt
    51181 tcttggatct tcctattgaa cagccgatca tacgatcgaa aattacttcg tactacccat
    51241 cgaagggata caagtaaatg gaaaagaaca aacatgagaa cagcttactg aactctccca
    51301 atagtgaggg aaatgtatgg ccgaagcagg tttgcccggc ttttgagccg agatcggata
    51361 ctccgaaaga gttacgccag tgctggtatt gccaattcgc tgattttcat ctaaacaagc
    51421 cgcgttcact ggatgtgggt gtctgttatt ggccaaataa agttctgagc taaaagagat
    51481 gcaagaacga attaaaataa cagtatttat gcttctttat gaacttgggt atactgcttc
    51541 ttcctcgcct gctttacagg cgaggaagtg gtaatacatt gggctcggtc gggaaccggc
    51601 agtcccctca tttggcaaga aaataactcc tcaccttttt tgtccggcgg tttccgttat
    51661 cgataggatt cctcataaat tttcaggcag tcttcatcgg ataatacagc cggatcaaca
    51721 ttgaaaaggc cgcccatggt atggcgcgca tttttaacga cttccggtaa ctcatctttc
    51781 gtaatcccgt aatcggacat cttcaacgca tccacaccgc atttcttttg taaatcgaca
    51841 agcgcttcaa caaaatccat cggttctttg gcatctttct tacctaatcc ttttgccatc
    51901 gcaatcaact tttcatcgca ggctcccgat ttggcaaaaa atgtgtaata tgccttgctg
    51961 atcataatca atccggctcc gtgaggtaaa ttatgatgaa aagcactgag ggcatgttcg
    52021 agtgaatgtt ccgaagtgca tccggacagg ctttccacaa tccccgaaag cgtatttgcg
    52081 agcgcgacat tctcccgagc gtctatatcg ttaccgttcg cgaccgcttc ggcaagattt
    52141 gcgccgatca gttcgatgga tttcatggcg aacatatcgc tgataacatt cgccgttaca
    52201 ttgatgtaac cttccgtgct gtggaagagt gcatcaaatc cctgataggc agttaatgcc
    52261 ggcggaaccg tcaacattaa ttccggatcg acgatcgaaa aatacgggaa tgtcgaatcc
    52321 caaccggatc cgattttttc gtccgtatct tctttggtaa taaccatcca gggatccgct
    52381 tcggttcccg ttccggcggt ggttgtaatt gcgataatcg gcagcggctt cttttcaacc
    52441 ggtttgcctt tcccgctgcc ggctccgaca tagtcccaga aattgccatc attcgtagcc
    52501 attaccgcga ttgccttggc ggaatcaatg ctgctgccgc ctcccaagcc gattacaaaa
    52561 tcacagcggt tgtctctggc gactttggca ccttctgtca catgagataa aatcggattg
    52621 ggaagaattt tgtcaaaaat aacatgctca atcccggcca tgtccagttg tttttcaacc
    52681 cgacccaaat atccgttttg acggatcgat tttcccgccg aaatcacaat cagtgctttc
    52741 tttcccggaa agttttgcag atgtaaatta tttaattgtc cccgaccgaa taaaattcgg
    52801 accggatcat aaaaactgaa gttcattgtt gcttcctcct atctgctttc ttaattacgc
    52861 cctaatcata tattatttgg gttaacaaag tcaaggctta aatccatctt tatgcaagaa
    52921 ttcatcgctg cttgagacga aaaaaatacg aatccgaaaa aatccggatt cgtaggatac
    52981 accggcacag aaaaaattat ttttacagaa gttattgtat cgttattcga ccaaccctaa
    53041 acgaatcaat tgctcatcgg gagcatgaat ggaaagaaga tagcttttta atttttcctt
    53101 cagttcgttc actttatccg gacgttgatg gtaaagatca tgctcctgac agggatcatc
    53161 tttgatatga tagagcgcat ttgacatcgg tccgcgagta acaatcgccg aataccgctt
    53221 gccgtcaaaa tcatctctga ctttaacatc cccgaattga ggaggtaaat gggaatacca
    53281 ataaagcggt tcgttactct tagtcggcgg ccataggaat aaagtccatt cgccgtcggt
    53341 aatgttaatc gattcaccgt agcgaccgta aaccgcgatt tcccgcttcg gaagatcttt
    53401 ctccgtcttc aaagtaaaag gaagcaaact gagaccgtgg atcccgtccg gaatcggtaa
    53461 ttcaaacgca tccagtacgg tcgcaaacag atcgaccggc tgggtaaggt tcgaaacccg
    53521 cttaccgctt tgctctttag ggttaaaaat gattaacgga atgctcgcaa tttcctgata
    53581 catatttgaa tctcccagat cggaccaagg tttcccgatc atcccgtgtt caccgaaaag
    53641 atgtccgtga tccgttgtca caataatcat cgtatcatcc atcaacccca gatgtttcac
    53701 cgtatcaatg aaatatccca accaacggtc aaccatcgta acttcaccgg catagagtga
    53761 gcgaatttgt ttaagctctt cttcgctgta taaatccgat ttgccgtatg tcggccaaat
    53821 aaaactttgt ccgttgtaac cggggttata catttcatta taaggaaacg gcggatcaaa
    53881 tggttcatgc gggtcgaaga cttcaagcat caaaaagaag tctttcaacg tccggttccg
    53941 ttccaaccat tcagaagctt tccgaaagac ctgaggggcg aaataatctt cttccaagcg
    54001 aaagtgggcg gtattccttt tatatcgcgc ataagattcg gatcgcaggg aatgccgtgc
    54061 cattcgttcg ggatcggcag aatattccac cggtatggtc ggatccgtaa tccacaaatc
    54121 attttcctgt ccgcgaatat attccgaacc ggagaaattg aaaaagtaat taccggaccc
    54181 gcgctcccac atatgataat gatcactgat gaccataccg gtatgcccgt tccgagtcaa
    54241 tatcttcgca atatctacat cttccggttc caacggaccc catccccgcc agggaaactc
    54301 gaggcagcct gtccagatat ctcttctggc gggcatgcag ggataggaac cgatatatga
    54361 actctcaaaa accgtgctgt tttcagccaa agcgtccaaa ttaggcgttt gaacccaggt
    54421 gtttccgtaa cagcccaaat aatcacgacg cagcgaatca atcagaataa agatggtgtt
    54481 cataagctct cagtccttta tctttccaaa cctaattcga attttcaagt attttcatca
    54541 catccgtcat cggccggtgt tgtcgaatta aatttgccat aatgcgaaac ggcgaatcga
    54601 catgcgcaaa aaaagtctta aaacccaaac aaaggtgatt caatccgggt tcgccgtcag
    54661 gcgtgatatc ggtccggtct ttcggacaac cgccgttgca ggcaaataag aaggagcaag
    54721 accggcaata ttggggtaac gaattccgtt ttgccaaacc gaattgcctc tgtttttcac
    54781 tgccaaccat ttccgccaaa ggtgttttca gcatgttgcc caaataacag tcgggttcga
    54841 cgaaatggtc acaagaatat acatcgccgt tatgttccgt caccaatgcc aaaccgcagg
    54901 tttcctgaaa aacacataag ccacccggcg caccgatcca atgaccgaag gaagtgtcaa
    54961 atatttgaac gaaaatttta cccaaatcgt gagcgagcca atcatcaaaa atcgagttca
    55021 aaaaatatcc gtattgctcg ccatttacag atcgctcgga aagcttccgt tccttttgcg
    55081 gatcagtcgg tctttcccat tcgacaatgg gaatgaactg aatgaattcg gcaccgactt
    55141 catcccgtaa aaaatgataa acctccaaag gtttctccgc atttgcggca ctgacacacg
    55201 tcaaaatatt aaaatcgact tggtagtctt tcagcaagcg aatcccgtga atgacccgat
    55261 caaatgtacc gtgattgcct ttatccttgc ggtagacatc atgtatttcc cgcggaccat
    55321 ccaagctgat tcccaccaaa aaatgatttt ccgcgaagaa tcggcaccaa tcatcgttta
    55381 ataaagtccc gttcgtttga attgcgttct cgattctcat tccgggacgt gccgcttctt
    55441 tctgataacc gacgaccctc cgaaaaaaat ccaaccccat cagcgtgggt tccccaccct
    55501 gccaagcaaa atgaacttgc ggaacctgct gcgccgcaat atactgccgc acataacttt
    55561 ttaaaacttg attcgacatt ctgaaagtgc cgccgggata taaagctttt ttgggcagat
    55621 aatagcaata ttcacatccc agattacaga ttgctccccg cggttttgcc ataacatgga
    55681 aagcgggagg tcttgaatca gatgcggttg tcactcgaat ctcaccgatg ataatttatt
    55741 ttcacataag gaagccattc tcatccccga catcttccaa ttttactctt ttaaagcacc
    55801 cattgtcatt cctttaataa tatatcgctg ggcgaacgca accatcaaca aagccggaat
    55861 catggcaaag aaggatccgg ctgccatttc gccgtaacga atactgtatt cctgcacaaa
    55921 gctggaaatt ccgaccggaa ttgtcatacc gcgtggagtt gtcgtcagag aaagtgcgaa
    55981 taaaaagtca cgccaagaga aaataaaggt taaagcgccg gcggaagcca agccgggttg
    56041 aataatcgga agcatcacct gagttaacga ctgaaaatgg cttgctccgt caacgataga
    56101 ggcttcttcg atctctttgg gaatctccgc aaaaaagtcc agcaatatcc atatagccgt
    56161 cggtaaagtt aagataatat gtcccataat aaccgctacc ggcgtatcat agatatttaa
    56221 cgctcgggaa attaaataga gtggacttaa gaaggtaacg ggcggcatca tatgaataaa
    56281 aaggataatt ccctgaatca atccgttcca taacggatgc caattaaacc ggctgaggct
    56341 gtaagcagcc aaactggcga tcaccaacac aacagccatc gttccggccg cgatcaataa
    56401 gctgttcgtt gtcaaccgca aaaaattgct ttgcgagggg ttcagcaatc gctcataatt
    56461 gattaaggtc ggtttaaata ataatgaacc ggaaataata tcccgaaaga gctttaacga
    56521 tgttcccaag gtaaaaaaga tgggaattaa agtgtaaatt accaccgcta aaagaaatcc
    56581 gtgtctgaaa agcgctccga aagttttctt tttcataggc gtgttctcct tccgaatcaa
    56641 gcgaggcggc ggccgatacg ccgataaaag ataacaaaaa cagagataat gattgccagc
    56701 gccaaggcaa gaaaagaggc atatcccatc ctgaattggt ttgaaaatac cttataaata
    56761 tagaggctga tcatttcggt cgcggtaccc ggtccgccgc cggtcagcag gtatatttca
    56821 tcaaatactt tgaaagcgaa aatggttcgc agcatcacag cggttacaag ggtcggcttc
    56881 aggagaggca gtgtaataaa gcgaaacacc tgtgcttcgg atgcgccgtc cacccgcgcg
    56941 gcttcattaa tttcctgggg taaagattcc aaaccggcca atataatcag aaacataaaa
    57001 ctggtccaat gccataagtc gaccaaaata atagaaggca aagccaattt tttatccgct
    57061 gtccaagtcg gaccggcaat tccgattaaa ccgagaacct ggttgataaa accgtagtta
    57121 tagtcataca tcagtcgcca catcgtaccg atggcaaccg gcggaatcag aagggggatc
    57181 aggaaaaccg tacggtataa ccccaccaaa tgatgactgc gggagaccag aagggcaatc
    57241 accaatgcta aaatggtctc gagaataacg acagcgataa caaaaacgat cgtatttctc
    57301 agagcaaccg ggacaatttg gtcttccaat gccgtgtaaa gatgttttaa tccgacaaaa
    57361 gtccaaacgg tctttccctg aacgattttc acttcggaga cactcatccg cagtaattga
    57421 accgtaggat aggctaccag aaaaatcagc acaagaatta agggaagaat ggtccatatc
    57481 ttaaaagtac gatcgataaa attactgaag cgagttttcc aacccggcgg tttggaagag
    57541 agggaaaaat atgtcgaatg atccatcatg atctccaaga aagttcatcg ttcaccgtat
    57601 atcacaggtc ccgatcggag cgataaaatc ctttcggatt gcaatttttt aagaatacaa
    57661 atttatttcc gaaggacgag aaggaggcag agtcaatctt ttcttttccg cctccttccg
    57721 aattatttat tcagccaaag gcttcacttt atagcccgca tctttcagaa tcttaaatat
    57781 ttcttttgcg gcttccgaca atgtttcttc cggcgttgtt tcggaaatta acgcctgttt
    57841 caaccggcga tcgataacct caatgatttg aggagtttcc accaatcggg gttgcgcatg
    57901 aatgaaaggt gtactgtccg ccatagcttt catccaccaa ccgactttcg gatcttgcga
    57961 catttcttcg tagacatcct gtcgaaccgg aatcgcaccg gcttgtgcat agtacagctg
    58021 agcatccttc gtcatcgccc atttgagaaa tgccaacgcc gcttccttcc gttccttagg
    58081 cagattatga ggaatgctca tcacccaaat accggacatc gtcgcatttg agccggcggt
    58141 agcacccgga acaggcacgg cggcaacctt gccctgaaca atagatttct ccggattatc
    58201 aaaatctgtc gcgactgcgc atgtcaaaac catttgggct gccttatcac tcgccatcag
    58261 ggcatccatt tcagcttggc cattatccgc gtaattcgcg ggaccgtatt caacgcccag
    58321 tttcagccac attttcaaag cttccaacgc ctgcggactg ttaatcgtca cttcccattc
    58381 gccggtttgt tcatccagct taataatgct ggttccgtaa ctggcaagaa atgcctgaaa
    58441 ttcccagttg gggggattcg cccggacaac atagcccgca aatttagaag gcttattcag
    58501 cttttgagca gccgctaaaa catcatccca agtttgcggt gtcgcaagtc cgtttgcatc
    58561 aaacagatct ttgcgataga agaaaagcat aatattccca ttgatgggta atccgtaaat
    58621 ttcaccgtct ttggtcgaat attttaattt gttatcccat cgggttgccc attcatattc
    58681 gatgatttgc ggatcgagtt caaaattcgg atcgatctct ttgataggcg tcaccaatcc
    58741 atccgcgtaa aactgcatat accattgctc attcaatgtg agaatatcga attcgctttc
    58801 ggaagcttgt accgcattgc gggacttctc gagcattcca ttaaacggga tacggttcaa
    58861 tgtcacctga ttaccggtat ccttgatata tttgttgacc aaagcttcaa aacccggata
    58921 ccacggagat tcattaatct gaatcgtaat gggaggcagc gcggcttttg cggtactcgg
    58981 taccggatta aaaccggaaa gaacaaaaga aaggatgagt atcagactga taccccagat
    59041 taatactggt ttttttatgg tttgtttcat agttatctcc ttcataactg actgaacgaa
    59101 tgaaaaaaac ggaaacatga aaaaatattt tagggaaacc tcctttctga attcggttta
    59161 gcggggagaa accgccgttg aacctcggat aatgagccgg gtaggaagaa tttgttgaaa
    59221 gggcttttca tcaatctctt tcgtgatcat tcgatagagt gtcttagcgg caatctgccc
    59281 ttttaaaaat gcatcctgcc tgacggatgt aagcggtggc gtcgagaatc gcgccagttc
    59341 aatatcatca aacccgatca cagagacatc ctgcggtacc tgccagccgg cttcacttaa
    59401 tccttggacg gcaccgatcg cgatcatgtc gtctccgcaa aggatggctg tgggagggtt
    59461 tggagatttc ataagttgta aagcaaggtt tctcccggca tccgaggaaa aatccatata
    59521 ataaataagg gattcatcga ccggaatgcc cttttccgta agcgccttct tatagccctt
    59581 tacccgcaac cgcactgaaa tcagattttc ggggcccgct aaaagggcca ttcgacgatg
    59641 attcaatgaa tataaatggt cgatcgcttg ttccattccg tcgataaaat cggaatccac
    59701 ccaacaggtc gggttgtttt cttcagcaat ccgacaggta aaaatagatg gaaaattatt
    59761 ttccgatagt tcgggaagat tcggatcatt caccggaatg ctcataaaaa tcagtccgtc
    59821 taccagacgc tgataatata aatgggagta tggcatgtcc ttatttgcaa gatgaatcag
    59881 caaattgata ttatgctctg aaagggtttc ggcgattcct ctcatcactt cattgaaaaa
    59941 cgggtgagaa aaaatataat tagccgaatg ctgaatgacc aggccaaccg tgttggaccg
    60001 tccgttgacc aaatttctgg cgattgtatt cgggtaatat cccaggtcat caattattct
    60061 ctgaattcgt ctgcgggtat cttcggacac ttccccatcg ttattaacca ctctggacac
    60121 tgtcttgatc gagacgccgg cgatatcggc gatatttttt aatgtgacca tttgtttact
    60181 gtcttgcatt ccgaaaaaat cctttcacat agaaaaataa tttattttga tttcgttctt
    60241 aaaaatataa ttgcgtagta taatgacaac gttatcgtca acgttgtcat tatactaccg
    60301 caaaagataa ggcatgtcaa tagacgataa tcattgattt tttgcagaaa gacagattca
    60361 aattaatttc gtaaattatt cggagacttg tcaattaatt ctcaggaatc ttcaggagga
    60421 ttcaaagtat gaacccattt tccccttcag caaaaatttt ggaagtacat gaaaccgaac
    60481 aaaaagggtt tcagccttta gtcacatttc aaacatggcg tgttgcgatg atgaccgatc
    60541 ttcaaagtga atcgccggag aaattttatc ggctgcaacg acatatgaaa acagatgaag
    60601 tttttatctt gcttcagggg aaatgtatcc tttttctggc agacggcgaa gacccgatcg
    60661 aacatcaatt cgccgaagat ttgaaattat ttcgggttta taacgtgaaa caaggcatct
    60721 ggcattccca tattttgagt gaggatgcca aagtcattat cgtggaaaat gccgatacct
    60781 ctccaagtaa ttctcagtac cttcagcttt cggacagtca gcgggaacga ttttctttta
    60841 tggcgaaaga gttatggggg acacttccgt ctgaataagt ccggtcgatt gttcgcaaac
    60901 gtaaccgaac tctaagaatt cccccgttca tgtttcgcat ccgatctttc cggaatgaac
    60961 ggaacagatc aatcgggaat atttgcgggg atacactcgc tttaaatctt cttattcaca
    61021 gaaaagcaac cgggcgattt atgacacttc tattcttttt tctgagctta gccgcttcga
    61081 caatcggggc aatcagcgga atcggcgggg gaattattat caagccggta ctggatgcca
    61141 cccatacctt gagcatcagt acaatcagct ttctttcaag ctgcaccgtt ctatccatgt
    61201 cttgcgtttc gattctgcgg aatcgcaaac aatccgtttc catcaattac aaaatgagta
    61261 cattgttggg catcggtgcg attatcggtg gaattctggg aaaagaactg tttgaaatgg
    61321 ccgtttctca tttcccgtct gaaaatatgg tgggggcttt tcaggctttt gcacttttaa
    61381 tcataacgat aacggtttat ttttatatcc gccggcaaaa aaatattcac accaaaagaa
    61441 tttcgaatcc gctgctcgtt attcttatcg ggaccctttt aggtatgatc tccgcctttt
    61501 tggggatcgg cggcgggcca ttaaatattg ccgttcttta ttactttttc tcgatgtcgg
    61561 ggaaaacggc ggcgatcaat tcattgtata ttatttttat ttcacaggta tccggtttat
    61621 tcaacctggt cgtctcaaaa agaatcccgc cctttgactg gtcgattttg cttctgatga
    61681 tgatcggagg cataatgggg gcgctcatcg gctatcgaat caaccgaaaa ttgagcgatg
    61741 cgcacgtaga aaaattcttc tgctgcgttt tgattttgat cggtatcctg aatatttgga
    61801 atttttatcg ttttgctttt tgaaattttg cgatattctt tttgcagacg gacagacgaa
    61861 tgcgaattct ttgttacatc cccgggaaac cttcaggaag gctccgaatt tccggttttt
    61921 tttaggaatt cgttctgggt tcggcacagc ttttgcgaat tttcaactgc gtcgatacat
    61981 acatgctcag ggtattttgg actgttcgat cgatattttt ttgtaaggtc gatacggcca
    62041 cctcggaaat ataatcgatc ggtatgttga ccgtagtcaa cggcggattt agaaatttta
    62101 ccgaggccag attattaaac ccgacgaggc tgatctgaga cggcaccttt attccattca
    62161 tggataaggt tgttaagaca ccggtcgcga cattatcatt cgccgcaaaa atcgccgtcg
    62221 gcagatcctt atgccgattt agcagctctt gggtcatccg cgatccttcg gtgaaagata
    62281 atcggcttcc ctcaaaaacc aaatccgaca catcgatttg atgatcgcgc atataagaaa
    62341 caaacatatc tttccggctg tccagcgtct tttcgcgatt attccccacc acttctccgc
    62401 cgataaaggc gatttttcga tgcccgagtg aaaacagata atccaatgcc tgttttgttc
    62461 ccaacttcgt gtttaccatg atggaatcaa atcgttcttc atccggagaa gaatcaagga
    62521 aaataatatt ggacgtaatt tttgataatt gttccacttc gctgatcaga aagcgcccga
    62581 tggcaatcat accgtcgact tttaaatcca cggagtttag ataaaccccg ttcacattca
    62641 caacccgaaa agtattgata ttttgcgagg cacagtattt ctcgacggcg gtcatcaaat
    62701 acaaataata cgggtcctcc gccaaatccg cttcactgta ccaatcgaca atcgcaatat
    62761 ttaatcgctc tttggcggaa gcctttcgac cgttcttctt tacatattcc aattcttcgg
    62821 caacgtttaa aattcttaat tttgtggcgg ttgtgacgct gattgtcgga tcatgattta
    62881 aaacacgcga gacagtagcg ggagaaacac ctgcagcttt agctatatct cgcattgtcg
    62941 ccatagaaaa ccctccgaat tcattaatcg ttctatatgg tacctgtttt ttttattttg
    63001 tccatatctt tatgagtatt atataactac ccgagagtat ttagtaaaca tttatctctt
    63061 aattagtaaa ttagtacgaa tgtaacaaga tatatattac taaataatat tgacctcccg
    63121 ccgtaaaacg attaaattca aggtacacaa gcgcttttgt gaaagaatat actttcagaa
    63181 atccaggagg gaaaatgaaa aaaacttttg tctgtttact tgtaattgcg ctgtttgcga
    63241 ccgttttcgg cgcggtcagc gctgagaatt cagacggtaa aattaccgtc tgggcatggg
    63301 atcccagttt caatattgca attatgaaag aagcggctgc ccgctacgag gcaatcaatc
    63361 ccgacgttaa gattgatata gtcgaaatgg caaaagcgga cgttgaacag aaattgaata
    63421 cgattctggc cgccaaaacc accgaagggc tgccagaaat cgtactaatt gaagactata
    63481 acgctcagaa atatctgcaa tattatccgg gatcatttgc ggatctgacg actaaattca
    63541 attacgatga tttcgccgca tataaagtcg gtgtcatgac gctggacgga aaagtctacg
    63601 gtgttccgtt tgattccggt gtagtcggat ttttctatcg ttcggacatc ttggcggaag
    63661 ccggcttcaa agcggaagat cttaagaata tcacttggga ccgatttatc gaaatcggca
    63721 aaaccgtaaa agaaaagacc ggcaaatata tgttgggctt ccagaatacg gacggcggta
    63781 ttatgcgcat tatgatgcaa tccgccggaa aatggtattt tgacgaagaa ggcaaaccgg
    63841 cattgatcgg caatcaggca ttggtcgagg ctttcgagct gtataaaaag attgttgatt
    63901 cgggaattgc caaacccacc aacaactgga acgaatgggt cggaaccatc aacaccggtg
    63961 agagcgcatc cgtgacaagc ggtgtttgga ttgtcggatc cattaaggca gccaaagacc
    64021 agagcggctt atgggatgtt gcgccggtac cgcgactgag caccgttgac agtaaaaatg
    64081 catccaacct cggcggttcg agctggtaca tcctcgacaa atcagccgat aaagatgccg
    64141 cgattgattt catgcagaag atttatgccg gcgataagga cttttatcag acgattctga
    64201 tcaacaacgg tgccgtaggc acctatctgc ccgccgcaac cggtgaagct tattcaactc
    64261 cggatgaatt tttcggcggc agagcgatct acgaagatct gagcgcttgg atgaaggaaa
    64321 ttcctccggt cgattacgga atctacacct atgaagccga tgcggcaatt atggctcaga
    64381 tggaagctgt ctgcgccggc accatgacga tcgatgacgc cattaaagca gccgaagcac
    64441 agcttgaaaa tcaaattcaa taacataatt ctttggattg ccactcacct cgccggtggg
    64501 tggcaatcac tttaaataaa ggatcaatac aaaatgggaa aaaagctcag catgaaaggc
    64561 tggttgttta tcagtccggc attgatactt ttagcggttt ttctggtata tccgatcata
    64621 tattcgtttt acctgtcttt tacatccaca aaaggcatca tcagttcttt tgtggggttt
    64681 aagaattaca ttcgattgac aaaagatccc atgtttttgt tggcactaaa aaatacggga
    64741 attatcttgg taatacaagt gccgattatg ttaatcagcg ccttaattct ggcaaatatc
    64801 ctcaatgatc gaaaattggc tggacgcggc attttcagaa cagccatttt tctgccttcc
    64861 gttacatcct taattgctta taccatctta tttaagatgc ttttttcttt tgacgggatt
    64921 attaaccggc tgctcctttc cacgggaatt gtcgatcatc ccctggaatg gttgacaacg
    64981 cccaaattag ctgtttttgt attaatactg gccatgcttt ggagatggac cggctataac
    65041 acggtgtttt acctttcggc tatgcaaaat atatcctatg aaatatatga ggctgcggaa
    65101 atagacggag ccagtaagac gcagaccttt ttgcgaatca ctttgccttt attgaaaccc
    65161 atgatccttt tcacgacaat tatgtcaaca atcggcacac tgcaattgtt cgatgagccg
    65221 atgaaccttt cgcaaggagg ggtgaccgcg gcaacgatgg ggcccgggaa ttgcttcctg
    65281 accttaagcg tatacatcta caatttgtgc tttaaatatg tccccaattt cggatatgcc
    65341 gcaacggttt cttacgtcat cgtctttatt atcgctgccc tgaccgtcct gcaattttgg
    65401 gtcggacaaa gcaatgaaga aaaagatgcc aagaaacgtt tggaaaagga aggaaaagca
    65461 tgaaaagaaa atttcgagtg agccgcattt ttctgtattt atttctgatc attgtttctt
    65521 ttatttgcgt ttttcctttt tattggatga ttgtcggttc tacaaatacg ccgattcaga
    65581 tcatcgccgg aaaaatgacc ttcggatcgc acttcgttga aaacttccat acgttattca
    65641 ataattacaa tatgttacgt atcttgatga actcattgaa aatcagcgga atcagtgtga
    65701 tcctgatcct attgatcggc gggatggcgg gatatggctt ccaaatgtat aaatccaacc
    65761 tgaaagaaaa actttatggt ttttttctgc tcacgatgat ggttcccttt gccaccttga
    65821 tgatcccgct ttttaaaatt gttaccaaac tgaacttatt ggacactcat tttgccgtca
    65881 tcatcgtttc cgcagtcagc gcgtttatga ttttcttctt ccggcagagt ttcgtaaatt
    65941 atccgacgga aatcttagaa gcggcacgcg tagacggggc gggtgaattt cggattttct
    66001 ttaatatttt tgtcccttcg atgaaatcaa cctttgccgc agcggcaatc tattctttta
    66061 tcaccagctg gaattcgtac ctgtggccat tgatcgtact gcaatccaat gataagaaaa
    66121 cgacaacgct cctgatatcc atgatgtcct cctcatatac acccgaatac ggagtcatca
    66181 tgctcgccat cgttgttgcc actctgccga ccatcattat tttctttgcc ttccaaaagc
    66241 agtttgtgca aggtatgctt ggttccgtca aacaataagg ggcgattgaa gatatgaatc
    66301 gaataaatat gcacaacctc gcatggctgg aagatccgga aatttttgcc gttaatcgta
    66361 tcgcagccca ttcggatcat gatcggatcg acgaagcggg aaatattatt ccaccgtttt
    66421 ctttaaacgg gaaatgggat tttttgtatt atgagaaacc cgatgacgtt gattttgatg
    66481 ttatttttga ttcaaatcat ccagccgatt ttaagtctgt aatcgtcccc gggcatattc
    66541 agctgcaggg gtttgggaaa ccgcaatatg taaacaccca atatccctgg gatgggtatg
    66601 aaaaaccaat ccctccgcaa attcccaagt atcaaaaccc ggtgggattc tatattcgtt
    66661 cttttgattt accggatcat tttgatcctg aaaacactcg tatttcattt cagggtgttg
    66721 aaagttgttt ttatgtttgg ttgaacggcg gttttatcgg atacagtgaa gacagcttca
    66781 caccggctga atttgatcta agcccatttc taaaaaagaa ggataaccgg ctttgtgtta
    66841 tggttgtacg gttttgcagc ggcagttgga tggaggatca ggatttttgg cgattttccg
    66901 ggatatttcg cgatgttttc ttatatcaaa cagctccggt ccatattcag gatatcgaaa
    66961 taaaaacaca tttgaatgat gatttcagcc tggcacaggt ggaagcaaat attcttctgc
    67021 tgaataaaaa tccccgagac atcaccgttt cattttcgtt ggcggatggt aaactgacaa
    67081 agtcgataca agcggaagcg ctttatgacg aagaaaattg ctctttttca atggagatta
    67141 aaaatccgaa actctggaat gcggaggaac ctcatcttta tcgggcggat attagcatcc
    67201 gtgaggcaaa atcgggcgat tttatttccg cggctcgcac ggaaatcgga ttccgcaggt
    67261 ttgaaatcaa aaacaaaata atgatgctca acgggaaaag gatcatcttc aaagggatca
    67321 accggcagga attctattcg aaaaaaggaa gagcgattga tgccggtgat atcgaagaag
    67381 atctcaaaat attaaaacgg aacaatttca acgctatccg tacttctcat tatcccaaca
    67441 acagtgcttt ttatcgctta tgcgatcgat acggattcta tgtaatcgat gaagcaaatc
    67501 tggaaaccca cgggacctgg atggtcctgg gaaaagtgaa tcgaaccgaa tttattgttc
    67561 cggatgataa acctgtttgg gaagccgctg tcatagaccg ggcaagatcg atgttggaac
    67621 gcgataaaaa tcatgcctgt attttaatgt ggtcctgcgg caacgaatct tacggcgggc
    67681 cggtgatcaa agctatgtcg gattggtttc atcaacgcga ttcttcacgc cttgttcatt
    67741 atgaaggaat ttcattcgac cggcgttatg atgataccag cgacgtagaa agccggatgt
    67801 atgccaagcc ggccgaaatc gaaaattatt taataaacaa cccccaaaag ccgttcattc
    67861 tctgtgaata cgctcattcg atgggaaatt ccttcggaaa tgtacaaaag tatatcgatt
    67921 tgacagaaaa gtaccctctt tttcaaggcg gatttatttg ggattttatg gatcaggctt
    67981 tgctgacaaa ggacgaaaga ggaaatgaat attttgccgt cggcggtaat ttcgatgacc
    68041 aaccgaacga tggatatttt tgcggcgacg gattgttgtt tgccgaccgg agtccctcac
    68101 cgaaattagc cgaagcaaaa tttttatatc aaccggtttc gataacctgt ctaccggaat
    68161 ccatccggat caccaatcat cagcttttta cggatacctc gggatatcgc tttgaatggg
    68221 tgttaaccgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    68281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catatcatcg
    68341 acggtgatta caatgtcggc attaaaatgg agaacagttt tgcgcttatt tcaaaagcca
    68401 acgggaagct cgtatccatt caaagcgaag ggacggagct attaagaagt ccgatctctc
    68461 cggatttttg gcgtgcaccg accgacaatg atctcgggaa cggaaacacc tttcgctggg
    68521 cacaatggaa aatcgcttct ctttatcaga aatgtgagcg tgtctccgtt gatcctgagc
    68581 gagcacaagt cacatccttt ttcttcatgc cggccaatcc caaaattcac tgcagcatgg
    68641 aatactcctt ttttaaaaag aatatcgttc ggatccgaat gcatctggat ccggtgaaag
    68701 gagacgctcc ttgtgtcggg attatgttca agatgcccgg aaaatggaat cggttatctt
    68761 ggtacggaaa tagccaatcc gaagcctatt ccgatcgtca atcggcttgc cggattgaac
    68821 tttgtgaagg atcggtcgac cggcaatatg tcccttatct ccacccacag gaatgcggta
    68881 acaaaactca attgcggttt ttcaccgtga cggaagatcg cggcaaaggc ctgcgtcttt
    68941 cgagtgacag tccctttgaa gcatccgcct tgccctacac cgctcatgaa atcgaaaatg
    69001 caagcaatat tttgcactta ccgcctcgga acgaaaccgt agtcggtgcc tataaacaga
    69061 aatgcggtgt cggcggcgat gacagttggg gagcgccggt tcatgaagaa tatttaataa
    69121 aaacagatga gggaatcgat tttacgatct accttcaaat tctttgagga atctttttat
    69181 cagccttttg agcgaatcaa gaggctgata aaaaatccgg aaataatcac tttcctttct
    69241 cgatcaaacc tgattacagg aaataatcat tcagccggcg ataaaaactg tcacttttag
    69301 ctgtccttta tttataaagc ttccacggct tgtaaccagt tctttgcgat aagcgcatgt
    69361 ccggcaagcg agggatgtac gccgtccggc aaccaaaaat tcgcttccct cttagccgct
    69421 gcctgggcga atattccgtc aagcggtaag aatatggcgt taaattccgc cgccagtttt
    69481 cttaccacag atatcttggg atccaagtct tctcgccatg cttccctatc ttcagacaca
    69541 ggtaagacaa acggctcaca taaaatgagt ttcgcatgaa gtttatctct tgtttgccgc
    69601 aaaatatagc gataatcggc ctcaaaattt tctttcggcg tcgggtcgtc gctgtcatat
    69661 cgtctccaac aatcgtttat cccgatcagt atcgataccc atgtgggttt tctgtcgatg
    69721 caatccgtcg tccatcgttc ttttaaatct cttaccctat ttccgctgat tcctttgttt
    69781 ataaacttca cgcccttctc cggataggta gcggaaaaat aagatgccgc catcatggga
    69841 tagcccgttc ccaaatcggt ttcatccgta taatcgcttc ccgcatcggt tacgctgtcc
    69901 ccctgaaaca acactatcgc atcatcttcc aacaacatgg tattttattc ctttcttgac
    69961 atttctccgt ctggctatac ttccctatcg attgtatccg ggaaaaatag gtttcattga
    70021 ctatgatctg cactatccgc agctgtctga acctaaattg aaatcttttt tccttgttcc
    70081 ctccaaaggt actctttcta tcttttgccg ttttgattaa tcctattcgt agttcattct
    70141 tttaaacaaa tcatattctt tatataaaat caattcccga ttttatcccg atcaattctt
    70201 catttttata cttgacaaaa ttgtatgaat agttatacta ttcatactta ttatactatt
    70261 cttactaatc ataaaagagg caggcaatca tgagcgctcc aaaaggaaaa tacgcatgga
    70321 cagtaaaagt gggagaaaag ggacaaattg taattcccaa agaagcacgt gaaattttca
    70381 atatctcgcc cggcgataca ttgattttgc ttggcgataa aaaacgagga atagcgattc
    70441 ctcccaaaga tgtcatactg aaacttgcgg aagtcttcgg agattccttg cctgatcagg
    70501 atacagaata atgatcgcta tacaaactgt tggtctgaca aaaaagtatg gcaaaataac
    70561 tgctgtagac aatctcaata tttctgtcaa taaaggtgag ttgtttgcct tgctcggtct
    70621 gaacggagca ggaaagacca caaccattaa aatgctttct tgtctgacac gtccaaccgg
    70681 cggagatgct attctgctcg gtgacagcat tatctctaag ccattatcga ttaaacaaaa
    70741 gacaaatatc tcaccacagg aaacagcgat tgctccgaat cttactatcc gtgaaaatct
    70801 tgaactcatc gccggtattt atggacaaga caagaaaacc gctcaccaga actctatctg
    70861 catggcaaat agattcgaat tggacaattt aatgacgaaa aaggtaaaag ctttatccgg
    70921 tggcttacaa agacgactca gtatcgctat ggctttgata tccgaaccgc aaattctatt
    70981 cttggatgaa ccgactctcg gacttgacgt gttggcacga cgtgaattat ggtcaacgat
    71041 taaatcatta aaagggaaag ttacgataat tcttacgacg cattatatgg aagaggttga
    71101 agaactttcc gatcgaattg ggattatgtc aagcggaaaa ttactcgcca ccggtacggt
    71161 ctccgaattg atggagcaga caaacacaaa caaatttgaa gatgcatttg ttatcctcgc
    71221 aaaaggtgta cgatgagagc ttacattttc tcaaaacgaa atgggaaaga aattctgcgt
    71281 gacccgatca atctattttt tggattgggg tttccgctca tattgatgtt tttattttcc
    71341 ataattaata cggcaattcc tcctgaagca aacaacacaa tgttcgagac gaaacgaatt
    71401 gctccgggca ttgccatgtt tggaaccgtg tttttagcgc ttttttccgg aacactgctt
    71461 gcgaaagaca gaacttcgtc atttcttatg cgcttattca gctctccaat gcgctcattt
    71521 gacttcatta tgggatacac cctgccgatg atcctatttg cagtcgttca atcgagtatc
    71581 acactgatag ctgcttcttt catcggctta ccgctaacaa taaatacgtt cctcgcagtt
    71641 tttgtcctcg tgccgatttc aattttattt gtaggggtgg gcttgttgtg cggtagcata
    71701 atgaacgata aagcagtcgg tgggatttgt ggcgcattac ttacgaacat cgcaggttgg
    71761 ttttccggaa cttggatccc gattgacctg atcggcggcg gattcaggac ggtttcccat
    71821 ttcttaccgt ttttccatgc tgcagaaaca gctcggttgg cgattgaagg aaaatatgtg
    71881 gatattttgc cgcatttatc gatcgtgctt gcttattcgc tgattgtcta tggaattgcc
    71941 attttcgtat ttaggtataa aatgagcagc gatagagcct aaattttgca tttccaattc
    72001 gactcttcta atccggcaac atttgcgaaa cctgcgctcg atttgcatcg atttcgcttt
    72061 ttatgatgga agcgattgat tcgaaaaacc tcctatgaaa tccatgccat gatatgttta
    72121 gttagtctgc gttatgggat caagtggcgt caaggaagtc cattttcccg a
//
LOCUS       DAOSMM010000003        66900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_447_length_66900_cov_17.054170, whole genome shotgun sequence.
ACCESSION   DAOSMM010000003 DAOSMM010000000
VERSION     DAOSMM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 66900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66900
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_66900_cov_17.054170"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..972
                     /locus_tag="PK898_01155"
     CDS             151..972
                     /locus_tag="PK898_01155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU18670.1"
                     /translation="MEAEYRDLHAVVESYEKYDEDSRLSADNIRRLEFLTALVYMNKY
                     LEKGNSILDVAAGTGAYALYYAALGMQVTALDITPSYIEILKNKAANQHLQITSFVND
                     ARDLSMFSDNSFDCVFCMGPIYHLPNPEDREKVMRESLRVLKPGGLFYLAYINKHFIF
                     ALLASQNKQYLQEKWYQRIIEQGVMYSTDEDCFWTDTWFTTPGEIEKLAERYRLKKLN
                     HVAQDGVGRLLADEINSMRPEYYKRWAEYHIRTCEEPSILGISNHGLYIGRKNSD"
     gene            complement(1117..2298)
                     /gene="ftsZ"
                     /locus_tag="PK898_01160"
     CDS             complement(1117..2298)
                     /gene="ftsZ"
                     /locus_tag="PK898_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU18671.1"
                     /translation="MENNYQNYGESFARIKVVGVGGAGCNAINRMIEEGMQNLEFIAI
                     NTDSQALLASKASQRVRIGDKATRGLGAGGNPEVGHVAAEESRDEIKEALSGADMVFI
                     TAGMGGGTGTGAAPVVAEICREINALTVGVVTRPFSFEGPRREKAALEGIANLREHAD
                     TLIVIPNDRLLQIVDKKVSLKHAFSQADDVLRQGIQGISELITIPGLVNLDFADVKAI
                     MSEGGSALMAIGTGKGDDRARAAAEMAISSKLLDITIDGARGILFNVTGGEDLTLYEV
                     NEAAAIIKESAHPDVNFIFGAVINPDMKEEIRVTVVATGYDQPLPNPTLSNSAVKYSY
                     SESLFNRRPSDSVRTNATPLTEKKDKKIPFSSNKEEDIPEFLKKDELDVPPFMRLMTK
                     G"
     gene            complement(2431..3654)
                     /gene="ftsA"
                     /locus_tag="PK898_01165"
     CDS             complement(2431..3654)
                     /gene="ftsA"
                     /locus_tag="PK898_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU18672.1"
                     /translation="MEKSIVVAIDVGSTKICTLIAQIEDENSIRILGVGIEPSEGIRK
                     GTVVDIPQAANSIAKSVKKAERTSGLEITNAIVSVAGTHISSINNKATVGVVGGVIDQ
                     YDVARVVEAVRAIPIPHNRVIIHLLQRTFSVDGVDGIRSPIGMYGSRLDLEAHIITAS
                     ASSIENLRQCIMTAEIDVNQWVLNPLADGEAILTDIEKQMGVVVCDIGGGTTGLAIYI
                     NGDVWHSNILPVGGGHITNDIAQAFHLPLSQAEEVKKIHGHCIPDFIGDEEYFYCRTF
                     GQDEAAKFSRKELSKIIQMRTQEIFQLVLQEIKRSGYDNLMPAGMVLTGGTSLLPGID
                     QLASDELHMPVRLAKPENITGMIDQLDSPAFSTSVGLLYWAMMMQETVGGQPKKKDVS
                     DTMDKIKKFARYLLP"
     gene            complement(3659..4504)
                     /locus_tag="PK898_01170"
     CDS             complement(3659..4504)
                     /locus_tag="PK898_01170"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOU18673.1"
                     /translation="MASIFKKGTDKKDEQPEISLLTMQSHKRYGTPIVSGVKNPSRKK
                     RKNARIVSAVLTVLLSCAAAAFSFLPQFRVIAPEVSGIEQVNMEEIIYYTGLRDIPIY
                     MSNPAQIRQVLLKRYPEIREMNVTVELPQTVRIEMLKRIPVIEWDFGGSKIWIDEDGT
                     ILKESPSASSDLIYVLSNSFPGAKNKNDRKFPEKFSKKMMQSVIAINQVKPQGKKLYY
                     TYDNGYGWETDYGYTLWVGIDDSELTEKMKMAESIEKYFKENEIQPDMLSLEFTQAPY
                     YRYAE"
     gene            complement(4523..6805)
                     /gene="murC"
                     /locus_tag="PK898_01175"
     CDS             complement(4523..6805)
                     /gene="murC"
                     /locus_tag="PK898_01175"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU18674.1"
                     /translation="MKIHDGMKIHIIGIGGTGMSAIALVLAEAGAIVTGSDRSRSDIV
                     SKLEDAGIKVFLGHRSENIGNVDLVIYSSAIPKDNPELREAQRRGIPVKRRIEFLNDL
                     LEDRDVIAVAGTHGKTTTTAMIAWLFSESGSEPGFIIGSTPKNLGKNAALGKSKEFVI
                     EADEYDNMFLGLFPSVAILTRVEHDHPDCFPTMDLYLNAFRKFISQVKPGGIILMNAD
                     DPNQQLLKDKMPADVSVYTYGESISADFRATDLEIGLDGCYKFNFLDSAKNRKLPIQL
                     GIPGKHNVFNATAATACCALKNLDLSKVSEALKAFNGIARRFEISAAWNDITIVDDYA
                     HHPTEIQATLSAAKDAFPNRRIWALWQPHTFSRTQTLLPKFIKAFDLADKLLITNIYA
                     SRETQTDFGFEDLKKAMINAYPDGLFAETNEQAVEILMNELQPGDVVITLSAGDANQI
                     GPKALERLRAAANDSVKKPYGVILKNEPIAKYSKAMCGGPAKELVIVRSKEDLVSIIK
                     EYQSSGKPFKVVGGLSNILFSDNGFDGLLVINQSDEISFEPQEEKVLVTVDSGAELNR
                     FVKACAEESLTGAEWASLIPGTVGGAIYGNAGAYGGDISHILRSVQFLTDNNEIVDLR
                     NEDLGFGYRTSKFKSGELKGTILSGTFELSRGNYDEIHEKMAVIAEKRQKFNFHGNGS
                     LGSVFRNPQGNYAGKLITECGFNGKRFGNVKVMDDHGNIFITYPGVKSSEFIELIHRV
                     HDAVWERFSIDLTTEIEIMN"
     gene            complement(6808..7911)
                     /gene="murG"
                     /locus_tag="PK898_01180"
     CDS             complement(6808..7911)
                     /gene="murG"
                     /locus_tag="PK898_01180"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOU18675.1"
                     /translation="MRLLICAGGTGGGVYPALAVVQERDKNHSDAILWVGSEKGMEYS
                     LVKHAGLEFRGIAAAGIHGVGWKKLPKNIASLVRGFFDSRRILKDFKPEVLFFTGGYI
                     GIPMAFAGIHIPQLIFIPDIEPGLSLKVLARLADTIAVVCDDSKKYLPSHKKIAVTGY
                     PTRAELRKWTKENARNLFGLSDLKPVLFIFGGSKGARSINQAVANHIHELLNLCQIIH
                     ITGQSDWDYVCKVREHLDPAEAKDYHINPYLHEEMGAAFAAADLVISRAGASTLGEFP
                     LFGLPAILVPYPYAWRYQKVNADYLAEKGAAIIIEDTMLDKEIVGTVGDLMQNRDKLA
                     EMKKAMSSLAIPDAAKNIAVLLEELAISKSEQS"
     gene            complement(7835..9073)
                     /locus_tag="PK898_01185"
     CDS             complement(7835..9073)
                     /locus_tag="PK898_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOU18676.1"
                     /translation="MGNAADVEFLKTKQARVSTKLLIDVPILVTVIILLILGLLFLYS
                     ASWNFALQEYDNAGYIVIRQAIWVVLGLVLATILTFMDYHFIRNIAQLIILGTIFLLT
                     LVLFIPSSIQGATRTVLNGSVQPSELAKIALIIYLAYWLSNKQDKLSTLVWGFFPVIS
                     IIGIISVLVLLQPDVSATITIIFLGAVMFYVAGGNPKHLGVILLLALFIFLCGYFLFD
                     KVSTRINDYVNGLKDPSLASYHIQRSTEAILRGGLTGVGLGKGVVKLTGLPVAWTDSI
                     FTVILEETGLLGGTFIILLYLVLIWRGYEISVNAPDTFGKLLAAGVTLWIGIEAFINI
                     GVMVNIVPFAGNALPLISSGGSSLICTLAGIGILMSVSRSSAIEALKKERKTPDALID
                     LRGGNGGWRISSLGRGSRKR"
     gene            complement(9052..10443)
                     /gene="murD"
                     /locus_tag="PK898_01190"
     CDS             complement(9052..10443)
                     /gene="murD"
                     /locus_tag="PK898_01190"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOU18677.1"
                     /translation="MENSWQGKRILMLGAGRQGQALARFMADRKAIVTISDIQPFDQL
                     RSAWESLKMLPVRWVTGGHPQSLLDETDFVCVTGGADLKQPILKEAFERGLAVLNDTQ
                     IFLTEVSAPVIGITGSSGKTTTTTLVGRIAEAAKKPDQKVWVGGNIGMPLLTMTDKIQ
                     PDDIVILELSSFQLELTTISPHIAAVLNITPNHLDRHETMEAYIRAKKNILNYQSADD
                     IAVLNRDDENSWHLRNDLKGKIISFGFDKPAENNGDPVIYCENMAIKREMNGEIENLI
                     RLDQIRLRGRHNIANTMAAFAVSTAAGFGIEAMRSAVVNFTGVEHRLQYVRNFHGADW
                     YNDSIATAPERTLAAVRSFDEPLVMLLGGRDKKLPWDELATEIHHHAHAAILFGEAAP
                     LIKKALERNKTSESELEIIQVDSLEEAVDAAASVCREGDVVLLSPGGTSYDAFRDFEE
                     RGKAFTEWVMRLT"
     gene            complement(10485..>10886)
                     /locus_tag="PK898_01195"
     CDS             complement(10485..>10886)
                     /locus_tag="PK898_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU18678.1"
                     /translation="GVLALMVGQAYIGRFCFILVGALFGFLWFNVNPAELFMGDCGSL
                     SLGATLAVVALITGHILVLPIIAIIPVSEAVSDIIQVVYFKKTHGKRFFRMAPLHHHF
                     EMLGWSEVQVVQRFWLINLLGVLLGIAIAMI"
     assembly_gap    10887..10986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10987..11632)
                     /locus_tag="PK898_01200"
     CDS             complement(<10987..11632)
                     /locus_tag="PK898_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU18679.1"
                     /translation="MKDSSLTLALSILGFMMTFIWGKPLIQLLHRFNIGKLVRIDGPD
                     THFAKIGTPTMGGVMFIIPTLLITVLLNAASLFGVQIIGQSFVLPMLTMIVFGLLGAI
                     DDWEGIRGPRRGLGMRARTKFILQVIFAIVIAWMMKHFLGVSHFYWPAFSEPIDLGNF
                     FIPIAVFLIVGMSNAVNLTDGLDGLAGLIAATAFAGYGVLALMVGQAYIGRFCFI"
     gene            complement(11629..13041)
                     /gene="murF"
                     /locus_tag="PK898_01205"
     CDS             complement(11629..13041)
                     /gene="murF"
                     /locus_tag="PK898_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOU18680.1"
                     /translation="MFSLNEILEALTGQQLAEKNRFFSEAVVDSRQAISGSMFVAIAG
                     ENTDGHQFIGEAFRRGALAALVQEDTSDKFTTVDLRTEKLPESFPPFPFCLRVDNTVA
                     ALQKTAAFHRDRLNLKVIGITGTVGKSTTKELIAEVLSQKYHTLKNTGNLNNEIGLPL
                     TLLRAGKGHQVAVLEMGFYVPGEIKLLCDISKPEIGVITNVGTVHAERAGSIEVIAAG
                     KSELVQALPKDGYAILNYDDPWVRPMAEKTSAHVFYYGLDPKADLWADQIESHGLEGI
                     RFCFHYQNESFFVRIPLIGRHSVHTALRAAAVGKIMGISWEDIFMGLNRGQSQLRMAV
                     MHSEDGAMIIDDTYNASPESVMAALNLLQEMDGYKIAILGEMRELGQYEENGHILVGA
                     RAAEVCDELIAVGEKTKKMVSSALKGGMDPQRVKWFATVQETIEYTRQRGFKKGEVVL
                     IKGSRGMQMERIVEAMERII"
     gene            complement(13125..14951)
                     /locus_tag="PK898_01210"
     CDS             complement(13125..14951)
                     /locus_tag="PK898_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU18681.1"
                     /translation="MNSNIDSKFKKLAICLSIFAVLVIGRILFLQTNASAVELKETIG
                     KAYEYTTLSVSSERGNIYDREGYLLAGNSISYTISLKLQASNTHGEFIANYLAPILGI
                     DREKIIQAAETPYVENEAVEVPLTNFATKEQVDKIELAKKSLNERNTTSFESKTRVFE
                     NLNAVVYYPNIYRYYPDGDLASNIIGFYPYLNPASGASFGIEQYYDDILSGETIQKKF
                     SLDPNVPESIPDLPKGASIALTIDRKVQKLVEEQVKLAVEQQKAKSGTIIIANPKNGE
                     ILAMATYPRINLNHYWESATTFTRENKFNPAVMQPYEVGSVFKVITLAIGIDSGVIKP
                     ETVFNDTGTFNVMGIDIYNWDRGAWGPQTMIGCMQHSLNTCLSWIAEQITAPVFYDYL
                     RKFHLDKPTGIELALEDYYPILQPGDEGWTEISLPTNSFGQGLMTTPIQMIQAVSAIA
                     NDGIMMKPHIVKAIYYNDHTEVIQPEVVGRPISAETAHTVTDMLETSLEVEASDALLD
                     GVKIAGKTGTGEIAKEGYGYVLNVTNASFVGWGPTDDPQFIAYVWLQEPLISIWGSEV
                     SAPLFSDVMNEIMPYLHIPYDRQRACLNSKTCPTEVPKNEYY"
     gene            complement(14948..15412)
                     /locus_tag="PK898_01215"
     CDS             complement(14948..15412)
                     /locus_tag="PK898_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18682.1"
                     /translation="MKTEQHAYRLAPWRKQLKSIINILLVLVLGACIASVYLNYSAQM
                     TDMKLQIQILHDQRNELTRTIADYVTREGILTSYALMEKKATQAGYIKIDFDDEDIYT
                     YLPIPGYIPDIDILSERRVQLPPLPVSPLKPEYTESLQQYIFRKLPTKESEK"
     gene            complement(15409..16368)
                     /gene="rsmH"
                     /locus_tag="PK898_01220"
     CDS             complement(15409..16368)
                     /gene="rsmH"
                     /locus_tag="PK898_01220"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU18683.1"
                     /translation="MTQETLSHQPVLLNEVVEALRPEPGKWYLDGTLGAGGHSEAILN
                     ASAPSGKLIGMDLDPNAIEIAAKRLAKFGERVKIIRGSYAFADRYITSCFDNITGFDG
                     ILLDLGISSMQVDAAEKGFSFQKEGPLDMRFDPNQKTSAYDLVNFASEEELSRIFWEY
                     GEERAARRIAAAICSARVREPIETTTMLAEIVANAVHTNRKSKNNPATKSFQALRIAV
                     NNELETIEKALPVLIALLKPGGRLAVISFHSLEDRIVKNFFRIESTDCLCPPEQVVCT
                     CGHKASLKRINRHAITAKDDEIRENPRARSAKLRVVEKIESEK"
     gene            complement(16365..16808)
                     /locus_tag="PK898_01225"
     CDS             complement(16365..16808)
                     /locus_tag="PK898_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOU18684.1"
                     /translation="MFTGIHEHTVDKKGRTCLPARFRNLLAEGAYISVGIDKNLVIYP
                     LPYFEKLANEISSFNLADPVARKYRRRMFNKAEPIEFDANGRFLIPPFLRDEFNFQDG
                     DKIIFAGSDEYIEIWTLDEWKKAEEEVFHDDSVTTEFARLMSNKI"
     gene            17225..18715
                     /locus_tag="PK898_01230"
     CDS             17225..18715
                     /locus_tag="PK898_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOU18685.1"
                     /translation="MESEYEVHHNDRELLIKIARETMEENDLLTEFPPEVIAELEQIN
                     EPAKPEGLPNIHDLRHFLWASIDNDDSKDLDQITVAEALSDDKVRIYVAIADVDSLVK
                     KDSAIDRYARYNTATVYHPAINFPMLPEKLSTNLTSLNPNEDRMAVVCEFTVDSEGTV
                     CEPKIYRAYVRNHAQLAYNSVAAWLEGQAEMPSALATVEGLAENIRLQNEVAQKLRKK
                     RYLHGALTLQTIQTIPIFDGDEVVDLAIDKPNLAKELIEDFMIAANSVSAGFLDECKF
                     PALRRVVRIPKRWDRIVDLAEQYGYKLSRKPDSKALNEFLIKEQQRDPLRFPDLSLTV
                     IKLLGSGEYVAEKPGEKAPGHFSLSVKDYTHSTAPNRRYPDLVTQRLIKAALNHEEPP
                     YSFEELDELAKHITAQEDLEAKIERKTAKAAAALVLENHIGETFNGFITGASEKGYWV
                     RILEIPVEGKLISGFSGLDVGDRVQVVLDSVDVEKGFIDFKRLKKR"
     gene            18852..19481
                     /locus_tag="PK898_01235"
     CDS             18852..19481
                     /locus_tag="PK898_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18686.1"
                     /translation="MAHFHEKLSSDSLKERMFWASILFLLLFFGVTILSFYLFPEGLL
                     KNKNPLQAWEPSESTLILTLQIFFYNLLSVLIIVLAGLFGTKKEGDANYLSIGYQAFY
                     TLICINSIVLGTWSFSIESQPVNLTARLLRTFDLVHRAGLWEMAGQLLITCSTAHIAV
                     ILSCNKNTVVKKIKDIRLSKSEKTVFITGILLMLIGAVVESVAIGGQVS"
     gene            19779..21764
                     /gene="nusA"
                     /locus_tag="PK898_01240"
     CDS             19779..21764
                     /gene="nusA"
                     /locus_tag="PK898_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOU18687.1"
                     /translation="MKNEFVLAFNEMLDEKQLPRETVIKALESAMVSAYRKAVTASTA
                     QHIEAKLDPDTGNFKIYAEKEVVDNVLDDRTEVVLAEARKVNPDAQIGDMVVVETTPK
                     DFGRIAAQTARQVIQQRLRDAERQDQIEYYEKKVGEIVSGVVQSISNEGYTIGLDRKA
                     EGLLLRKDTIKGEHFRLHQHVRALIVEVKDTSRSAQIMLSRTDRKFLFRLLEIDVPEI
                     YHGIVEVRSIAREPGMRAKIAVSATQQGIDPVGACVGMQGKRIQPIVKELHDEKIDVI
                     EWNPDPAVFIAKAISPAKVVGVYLGNNKNATVVVPEDQLSLAIGRDGQNARLAAKLTN
                     WRIDIKSVIESASEILGAIDSKKDIVEENQESGERTVIINYADLFEQEKDNIEAIRAI
                     LAKRAENRPISPDETETLNRFVDRIERQKVAGYNLSMVSEVIEKLSKQNLTEETLFAM
                     PVSASGLPKYVTNILYAGGFRTFGDLYNHMMSNPEEIYKLQGVTPRIMSDIASLVKFL
                     GSIGSKKTETEPVEVSEEQPTMEAQPVAEIAEAEELPEPVSFEKSERKYEGKAEDFVD
                     LTQLPLQTETKPAEPEPQTEYEDISFDELFNAMRNEIATPTEYLEDEDELKDDLTSTK
                     GKKKKKKKNVEIEYDPDSDSTLIHKKHKRGEDEDWGW"
     gene            21765..22064
                     /locus_tag="PK898_01245"
     CDS             21765..22064
                     /locus_tag="PK898_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOU18688.1"
                     /translation="MSNKSEKKHVRHVPQRTCIACRQTLPKRSLIRLVRTDEGLQLDP
                     TGKVSGRGAYLHDSKACWNIAIQHRLLEKSLKTELSDKDLEKMRLWMEDLPDNAE"
     gene            22110..23894
                     /gene="infB"
                     /locus_tag="PK898_01250"
     CDS             22110..23894
                     /gene="infB"
                     /locus_tag="PK898_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU18689.1"
                     /translation="MGDEAKRVQIPYTITVRDLAKLLQSNSIQVMRILMANGVMANIN
                     QLVDFDTAAIVASEMGFEAVPESKEQVEEFKETGDVPLWRQIINKEDPKSLIPRPPVV
                     TILGHVDHGKTSLLDAIRHTNVAVGEAGGITQHIGAYQIENKGKLITFLDTPGHAAFS
                     AMRARGAQGADIVVLVVAANDGVMPQTKEAISHAKAAQVPIIVALNKIDRSDANPDLV
                     KKQLSEQGLIPDEWGGNTMVVPVSAKQRIGIDDLMEAILLTAENSEILANPKGKVFGT
                     VIEASIDPKKGVMATLLVQNGTLKMGDVVLAGQSIGKIRAMFDFRGKKIQKAGPSTPV
                     QIMGLTEVPTAGDIFQVYASEKEARAVVENAKNEAALKSSTPKATLEELFAKFKAGEA
                     KELNLIIKTDVNGSLEPIINSINDLSKEGEIKINILHAEAGNISENDVMLASASHGVV
                     IGFNVQADPVAKKKAENEGVSIRIYDIIYRLIEDIEKALKGMLEPEFKETVIGRAEVL
                     TTFSISKVGTIAGCRVKEGDIRRNCKVHVKRGNEIIFTGEISSLKHEKEDVKEVKTGL
                     DCGIGLKGFEDFKPGDILEAFVLERFGM"
     gene            23916..24302
                     /gene="rbfA"
                     /locus_tag="PK898_01255"
     CDS             23916..24302
                     /gene="rbfA"
                     /locus_tag="PK898_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU18690.1"
                     /translation="MPSPIRLKKIAERIRDDLSEMLLYEIDDPRLQGVMITDVNVDRE
                     LYYANIYVSALEGKERSAEILAGLENASGYMRHALSQSIELRTFPKLRFFWDPTPEKA
                     DRVETLLRQLKEEQEAKNKNEQKGIA"
     gene            24428..25420
                     /locus_tag="PK898_01260"
     CDS             24428..25420
                     /locus_tag="PK898_01260"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOU18691.1"
                     /translation="MNQDKNNVLTEIRKIIENATNIALFCHIRPDGDAIGSMLGLGWS
                     LEEMGKKVQYVCPDELPVQGWLNPQTIGKNTRFVKAPEPYDCSVLLDVSTLSRAGYFF
                     EQPDIGQPDICIDHHVSNSGDAKINLIDPSAPATAIIVAHLIEELNFPMNKEIAEALL
                     SGIIMDTQGFSTSNTNPDSLRVTAKLMEQGADLYWGIQNILKAHSLEAAKYWHCGLSK
                     LETRGKILWTVLTDTDRVHSGYTGRDDAGLIDYLASTEGFLATVLFNEQNDGRVKISW
                     RSRPGVNVAAIAEEFGGGGHRQAAGADVNGPIDDVVERVLACTEKGVRSAEQSS"
     gene            25489..26403
                     /gene="truB"
                     /locus_tag="PK898_01265"
     CDS             25489..26403
                     /gene="truB"
                     /locus_tag="PK898_01265"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOU18692.1"
                     /translation="MRSEETKRYKSAISGVLIVDKPIGKTSHDIVQIIRKGTGIRRIG
                     HTGTLDPRASGVLVILIGPAVRLSEYLQSDIKRYEATIRLGSVSDTYDGDGLIETTGA
                     DMNISEDQISEAMKKFVGEIEQTPPVYSALKIKGRKAYEMAREGIDFEIPSRKVTVYS
                     MDLIEYNPPELSVDILCSSGTYVRSIANDLGQVLGCGAYLSGLRRTLSGHFSLRDAVS
                     IPNLEASFEEGNWYQYLIPAAEALDGYAELVVDMDEESQIRHGRRIPAQNTDSPYAKA
                     VSEQGELIAILELDPETSEWKPKKVLYP"
     gene            26411..27346
                     /locus_tag="PK898_01270"
     CDS             26411..27346
                     /locus_tag="PK898_01270"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOU18693.1"
                     /translation="MVQADLISQITLKASAITIGGYDGIHCGHRFLLDAMLKDAHQQS
                     LPTVMVTFDPLPFVFFQKPKFPKNIILPQERAEIAEQIGIDYLITLKFDQQLADQSAN
                     DFISELSESLGLKSLWVGEDFSLGKNREGSQETLKKLADQFHFALHILPKLEMDGGIV
                     SSTWIRGLITEGQVQEAAKLLGYPFFIENKIVHGAGRGKKLGFPTVNMQIPKGKIIPA
                     IGVYTTYIRLHGKKFPAITNVGTRPTFYNDHQIVLETFVFSSDGKDEYGETARVEFLE
                     YMRPEFKFGSADELVNQIRQDIRHAEAFFEKRNQL"
     gene            complement(27356..28141)
                     /locus_tag="PK898_01275"
     CDS             complement(27356..28141)
                     /locus_tag="PK898_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU18694.1"
                     /translation="MKNKLFILSFLFLFLRSFYPAVANCTNPPDKQHFQIFSESLNKN
                     IDFSVYVPPCYDKRLVKGYPVLYLLHGQNMDESVWDNLHLSEDLQAGWKDLTLPYFLV
                     ITIREEENLQDLFYSDFDRAILESVIPWVDQTYHTCTDPSCRAIAGMSRGALWAADIA
                     FNHPDIFGSVGLLSLPGSPFDDQTVHVLITQMKQKNGSLRVLIDSGSEDAYRMKSETF
                     CNQLTFEGFPYSAEIHFGGHDNGFWQSRLPYLLSWVSLIWTRN"
     gene            28248..28529
                     /locus_tag="PK898_01280"
     CDS             28248..28529
                     /locus_tag="PK898_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HOU18695.1"
                     /translation="MTDHLQSEDSKGFTFPCEMPIRIIGENTEAFEEEVIEILSHYLP
                     EVAPIDFRTRMSRANNYCSIAYTFYAESREQVEGIYRELSLSKHVKWVL"
     gene            28531..29601
                     /locus_tag="PK898_01285"
     CDS             28531..29601
                     /locus_tag="PK898_01285"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOU18696.1"
                     /translation="MSFRFDKKPDRRDFLKFVGLGAGALAFRPYYSETEFIPSGAKTV
                     RVAIANLSVYKEPWDESEILYQKFRDDLLNVYYEVNSEHGPGYNPIWYRVWGGYVHSG
                     HTQIVETRINEVDYSVNSTPLPGKIGEVTVPFTQSRLHNSNGSWEDVFRLYYQSVHWV
                     VGVQEGLDGRPWYRIKDEIFDADRLDYYVPAEHIRIVSDAEISPISPEVPWKEKRIEI
                     SIGMQQLIAYEYDRPVLKTKVSTGIPSRKIEGQISTSTPTGTFNIQDKMPSKHMGDGK
                     MTADLEAYELPGVPWVCFFEPLTGVATHGTYWHTNFGMTMSHGCVNMRNEEAKWIYRW
                     SYPQVTTHKVENIGYGTKVVVS"
     gene            complement(29603..30769)
                     /gene="hemW"
                     /locus_tag="PK898_01290"
     CDS             complement(29603..30769)
                     /gene="hemW"
                     /locus_tag="PK898_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOU18697.1"
                     /translation="MDKPISVYIHIPFCKTRCNYCDFNTFTGMESFIAPYLASVKEEI
                     RNCVISMEINQPVHSIYLGGGTPSLLSPEQIRELIQTVRDHFFVTRDAEITMEMNPGD
                     LLPEKTMMLRSTGVNRVSLGMQSAINEELKCMGRRHSVEQTLDTYHRLREAGFDNINL
                     DLIFGYPGQTMESWRYSLEKAMELEPDHISLYALSVEKGTPLESQILSGSLKALPDDT
                     IADMYDSACEMMKEHRYIHYEISNWSRTHQTESRHNKQYWYIEPYLGFGSGAHSYFSH
                     VRIKNVSSIEEYIERINFNRPWENTYPAAEEIIALSELTEMKDVVIFGLRLLQEGVNP
                     TKFKERFGLNLEDIFHYQLKKLLTNGYVYYNQEGNLVLNEKYAFVSNQIFLEFV"
     gene            30849..32009
                     /locus_tag="PK898_01295"
     CDS             30849..32009
                     /locus_tag="PK898_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU18698.1"
                     /translation="MAPDNLQQSRTKICPTCGTRMNESATRCLVCGHIFTGTEENKPS
                     PKKSKTSRNDPLQKKPRLPEVRLSLPIAIGLIILAIVIGAAVVFLFLQNMGRVVEPTP
                     LPTNTGTPTITFTPTTTYTPTLVMTATPLPPVEYTVKEGDLCSSIAALFNISIASIVL
                     ENNLTSDCYLSPGMVLKLPQPTPTVTPLPTNTLTGSEATLAACGSYDYIVGNTDTLFG
                     IALNFGVSTDIIRQYNGLMNDIVVAGSTLHIPLCERITPGPTSTPTNPPPYEAPNLLL
                     PADGSSFNTSGETITLQWAGVGTLLDNEAYEVNIEDITGNTGRKLVDYVKDTKYIVPM
                     SFRPSDPSPHVIKWYVQPVRQSGSQADGTPIYTSAGARSEARTFIWSGVAVQ"
     gene            32170..33534
                     /gene="der"
                     /locus_tag="PK898_01300"
     CDS             32170..33534
                     /gene="der"
                     /locus_tag="PK898_01300"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU18699.1"
                     /translation="MKRPVVALVGRPNVGKSTLFNRLSGTRDAIVNDVPGTTRDRLFG
                     HAEWAGVEFDIVDTGGIDPSPSGQKAPLSVDSAEFVQEIKSQALLAVSEADVVLFLVD
                     AISGITPADREIASILRKNQKIVDGKETPPILLVANKADSARQRQNVSEFYELGMGDP
                     FPLSAIHGTETGDMLDALIALFPKQEEETPDDSVKIAIVGKPNVGKSSLLNRIVGEER
                     AIVSDIPGTTRDAIDTHILYNGVPVTLIDTAGIRRRGKIEPGVEKYSVIRSMRAIEEA
                     DVSLLVIDALSDITSQDAHIAGYILEEWKSVVVVVNKWDAVEKDTYTMQTYTDKIRKE
                     LNFMPYVPILFVSALTNQRVNQVIPTALQVQEERLARITTSQLNEVIHDAQDKHHATS
                     RTGRSLQIYYATQVRSDPPTFLIYVNDPKLAHFSYTRYLENQIRKQFGFIGTPIRIVY
                     RARR"
     gene            33657..34277
                     /gene="lepB"
                     /locus_tag="PK898_01305"
     CDS             33657..34277
                     /gene="lepB"
                     /locus_tag="PK898_01305"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOU18700.1"
                     /translation="MAKKQPEKTNKNTKKKEAEKKKTTAGMIWEIVQTLLMAAVLYFA
                     IDFCIARVRVENISMEPTFTEGELLMVNKLNYKFSEPERGDVVIFEAPVAPGKDYIKR
                     MIGLPGDVVTVKDGKLYINGELINEPWVHEPIGYSGEWEVPENQYFVLGDNRNHSSDS
                     HSWGFVPRENLIGNAFFCYWPLNHIRVINSHVTLQNELETASLKGE"
     gene            34281..34559
                     /locus_tag="PK898_01310"
     CDS             34281..34559
                     /locus_tag="PK898_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOU18701.1"
                     /translation="MERVEYCEECQVGIPHFTTATYFTWIEDELITVPDFPCWVCDVC
                     GRREWDAESLMNLNLILSPNAGKPQPRKRTITGESKSTRAKTRRSAVR"
     gene            complement(34628..35893)
                     /locus_tag="PK898_01315"
     CDS             complement(34628..35893)
                     /locus_tag="PK898_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18702.1"
                     /translation="MYLKGSKYSYNGRKRRSNPFRVFFLFVLVAAGLYFSIYIAPETQ
                     PLFIPTNTPTTPPEAFISEAEQFAKEGRNAQAIELYQKAISSDPKNPSVYISLAKLQM
                     FETHYEDALKNAENAILLNGNNAVAHAIRGWALGKMGNYLDAETAFNRAAEIDPNNPL
                     TFAYRTEILVDQINAGQDSLNTLEKATEYSRLAIEHGNELMETHRARGLLLEITGNYE
                     DAINEFQIAISINPNISDLYLALGRNLRAAGKDSEAITAFERARSLNPYDSWAPYYIS
                     RTYSGNGEYAKAIQQAQDAIDLDPTNAMFYWNLGTQYYMSEDYANALTYFRLAIQGGT
                     TPNGEIIEGIPLSYDNRVPELYYMYGIILARSGNCQEAAEIVKLITQGIPNDTIAVEN
                     AQYMTDICKSGTFDITLTTPTAPAPAPAE"
     gene            36104..37126
                     /locus_tag="PK898_01320"
     CDS             36104..37126
                     /locus_tag="PK898_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU18703.1"
                     /translation="MKKISIILALIAILVFCLPALADDVIKVCEVTDTGGVDDKSFNE
                     TAWNGILQAKEKYGVEAVVLESQSDADYAVNIDQFIEDECDLIVTVGFMLMDATATAA
                     DNNPDFKFSIIDASVGKPNVVDQVFQTDEAAFLAGYVAAGMTKTGIIGTFGGMCIPTV
                     TIFMDGFARGADYYNQIHGTKVEVLGWNTETLEGTCVDSFDDLDKGKQVSITMMDQGA
                     DIIMPVAGPVGGGTIAAMEDRKMGMVIGVDSDWYLTYPDNGDIILTSVMKKMDATTMQ
                     VIESVIDGTFKGGKVVGTLENKGVDLAPFHDFEDKVPADLVKELDDIRAKIISGEIEL
                     NPVYAQ"
     gene            37306..38862
                     /locus_tag="PK898_01325"
     CDS             37306..38862
                     /locus_tag="PK898_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18704.1"
                     /translation="MPPILELKGITKQFPGVLANDHINLTLEKGEILALLGENGAGKT
                     TLMNILYGLYKPDEGEIFIDGEKIEISGPIDAIRAGIGMVHQHFMLIPVFTVTENVML
                     GEESVNFGGVLNPEDAAKKIKAIGELYGLKVDPNAKVGDLPVGVQQRVEIIKLLYRNA
                     RILIFDEPTAVLTPQEADELFEIMRSLTKQGKSIIFITHKLREVLEFSDRIMVIQRGK
                     VVGETHPHEASQEKLAEMMVGRAVDLIPDKKKIQAGETVLKVDNLVVADGTRKITVHG
                     VSFSVHAGEILGVAGVQGNGQTELEEALTGMREPVSGTIRILGKEVPKVKPREFVENK
                     GAHIPEDRQRDGLILNFTVEDNLMLCTYYQPPFSQNHILNREAIRDHARKLITNFDIR
                     PPEPLLPVASLSGGNQQKVIVARELSRDNKLIVASQPTRGLDVGSIEYIHDQLLKSRE
                     EGCGILLISSELDEIMELSDRIIVMYKGNITLMTETEKTTKSEVGLHMAGAGNSMSEK
                     SENVAVQAGR"
     gene            38880..40184
                     /locus_tag="PK898_01330"
     CDS             38880..40184
                     /locus_tag="PK898_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18705.1"
                     /translation="MDKENKGLNREQDAKKMSTEEIFLEELSRKKAPRPGFWAKFWDA
                     IQIPLLAIISGLFLGAVIIVVTSADVYVAFGSGIGEGFRTMGSIVGNAYKTLFTGAFG
                     SPSKIIEALRTGEKISRAFKPITQSLVETTPYLLSGMAVALGFRSGVFNIGGEGQIYI
                     GAVAATFVGLSFDGLPQAVHMPLAILAGIIGGAIWGFIPGWLKAKTGAHEVINTIMMN
                     YIALRLVEYLLTGPMTKTGGGGMPISNSVKPTARIPLVFAPPITLHWGFFLAILIAIA
                     AWFFLFKTTWGFNLQMAGANKHAAKYAGLNISLWTVLGMTISGALAGLCGSTQVLGVT
                     YSMGLGVSAGYGFDAIALALLANNNPLGCILSALLFGLLKTGSRSMMSNTGIPLDIVS
                     IIQAFILMFIAAPAIIRTVFRVKGLKEENKALVTVSGWGGDK"
     gene            40181..41452
                     /locus_tag="PK898_01335"
     CDS             40181..41452
                     /locus_tag="PK898_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18706.1"
                     /translation="MSEQERIQGGRVKLRDRIVGIIFVVLGLVIYLFFYKAIASGIVS
                     TFIMTPGGITRGVVGNWIIPARPVLIVLAMILIGIGIYQFFFGLGKQGNLFLALACVA
                     FVFSFLIYVSAGKRLNLASLFSSALSMAVPITLASFSGILCERSGIVNIAIEGMMLMG
                     AMVGSIVGSITKNIWLALAMAMIGGGLLALVHAWMSIKYKVNQIISGTVINIFSMGMT
                     SYISSMYLQNIQSLNKTPRFPSVSIPLLADIPFIGPIVFYNSLFVYGMILLMIILQFA
                     LFRTRWGLRLRSVGEHPKAADTLGINVYKTQYTAVILGGLMAGFAGAYFSLGSVGRFD
                     EGMTAGKGYIGLAAMIFGGWNPFNAMMAGILFGFADAMASSVSILGSSIPGQFINMLP
                     YVVTMIVLAGLVGGHSTGPAASGVPYEKEST"
     gene            complement(41579..41920)
                     /locus_tag="PK898_01340"
     CDS             complement(41579..41920)
                     /locus_tag="PK898_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOU18707.1"
                     /translation="MIDFDNRPWGRWEEYIFEPNYRVKRIIVFPGKRLSLQLHHLRSE
                     IWVIVQGSGKMTLGDKIFPIHEGDIVQIPKEQAHRVENDSDQPLVLIETQLGICPEDD
                     IVRLEDDFGRV"
     gene            complement(41931..42995)
                     /locus_tag="PK898_01345"
     CDS             complement(41931..42995)
                     /locus_tag="PK898_01345"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOU18708.1"
                     /translation="MKVAVLAGGNSPERDVSYSSGCMISNALAEKGHEVALIDVYLGI
                     STPPQGLQSLFKKYPNYQKQSYKIPTTPPDLEKLAAQRVSDTGMFFGENILELCRLAD
                     VVFLALHGSAGENGQLQATFDLLNIHYTGTGFAGCVLAMDKHLAKEVMVANDLPTAPW
                     VYLDRYDQLNWDQIDSLGYPCVIKPPRGGSSIGVSIPQNKEEFLRGIQAAIASAEDRQ
                     PDLLVEKYLAGREFCIGILEGISLPAIEIIPNEGWFDYEKKYQSGLTREVCPAEIPEE
                     LSIRLQDTALKIHRALKLGYYSRIDFKVDGDGNIYCLEANTLPGMTPTSLFPQEAAAV
                     GIGYADLCDKIVRNTERKEE"
     gene            complement(43337..44194)
                     /locus_tag="PK898_01350"
     CDS             complement(43337..44194)
                     /locus_tag="PK898_01350"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOU18709.1"
                     /translation="MSDQCSHNCSSCEETCNSRQKKPADLHEKPHAMSRIDHVIGIMS
                     GKGGVGKSLVTSLLAVKMQKLGYHAAILDADITGPSIPKIFGIQSRATGNDFGILPVR
                     TKSGIDIMSINLILNDSTDPVLWRGPIIAGTVKQFWTDVIWENVDYMFIDMPPGTGDV
                     ALTVFQSVKMDGIIIVTSPQDLVSMVVSKAIKMAEMMNIPILGLIENLSYFKCPDNGK
                     EYKIFGDSHIEQIAENYGIDILAKVPVDPAISEACDRGAIETLEEDWLEPAVQRISEQ
                     EKGKGNGRS"
     gene            complement(44346..45974)
                     /gene="groL"
                     /locus_tag="PK898_01355"
     CDS             complement(44346..45974)
                     /gene="groL"
                     /locus_tag="PK898_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055846.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOU18710.1"
                     /translation="MAKQLYFGEDARKNLKAGIDVVASAVATTLGPKGRNVAIDRKYG
                     SPTVTHDGVTVAKEIELPDPFENMGAQLLKEAASKTNDIAGDGTTTSTVLAHAIVSEG
                     LKSLAAGANPMLIKRGIESAAKVVSDEIKAQSIKITTKHDIANVATISAQDETIGSLI
                     ADVMDKVGKDGVITVEESKGLEFETEYVEGMKFDRGYISSYFMTDTDKMEAVINDPYI
                     LIYDKKISAAQDLVPILEKLVQTGKRDLVIIAEDVDGEALATLVLNKLRGMLNVLAVK
                     APGFGDRRKEMLQDIAILTGASVISEETGRKLETAQLSDLGRCEKVISDKDNTTIVGG
                     KGDAGEIKARIDQIRVEIDKTTSDYDREKLQERLAKLSGGVAIIRVGAATEVELKEKK
                     HRVEDALSATRAAVEEGIVPGGGVALLNAIPKVEALKFKDEDVQTGVNIVRKALTVPT
                     HRIVENAGKDGSVVIANIRAQQKAKKSKLIGYDVLKDEYVDMVDGGVIDPAKVTRGAL
                     ENAASIAAMILTTEALITDIPEPEKPAPAPAPEY"
     gene            complement(46009..46296)
                     /gene="groES"
                     /locus_tag="PK898_01360"
     CDS             complement(46009..46296)
                     /gene="groES"
                     /locus_tag="PK898_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOU18711.1"
                     /translation="MNLKPLGNRLVVEPIEEEEMTAGGIVLPETAKEKPQKGKVISAG
                     PGDRNEKGDRIQMDVKAGDTVLFAKYAGTEIKVDGKKLLILRESDVLAIID"
     gene            46498..47244
                     /gene="trmD"
                     /locus_tag="PK898_01365"
     CDS             46498..47244
                     /gene="trmD"
                     /locus_tag="PK898_01365"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOU18712.1"
                     /translation="MKFDVFTLLPGVFEDYLNSSILLKALENQLIEVAVHDIREWAPG
                     KHHVTDEPPYGGGGGMVMKPEPVFNAVEAVLGDKPDCPVILLTPQGKVFDQKTAQRLA
                     REPHLALICGRYEGFDERIRTHLATEEISIGDFVLTGGELPALVLIDAISRNIPGVLG
                     DPDGAMTDSHSEGLLEYPHYTRPAVFRDWEVPSVLLSGNHAAIENWRREQSLARTLER
                     RPELLDRAELSETDRQILQKLGWKPKERSA"
     gene            complement(47266..48378)
                     /gene="tgt"
                     /locus_tag="PK898_01370"
     CDS             complement(47266..48378)
                     /gene="tgt"
                     /locus_tag="PK898_01370"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU18713.1"
                     /translation="MNSDNFPFEIEGKDGKARAGVFHTPHGSFQTPIFAPVGTQATVK
                     SLTPVQVEEAGATLILANTYHLFLRPGDDLIAEMGGLHRFMDWQKPILTDSGGFQVFS
                     LDGMRKIDEDGVTFKSYIDGSVHRFTPEISIAVQENLGADIIMAFDECANPKDYEYNI
                     RSMDRTHRWLQRCIDAKTNDKQALFGIVQGGIFPDLRTKSAEFVSSFDLPGFAIGGLS
                     VGETKEEMNAVLDIVPEILPENKPRYLMGVGSPEDLINGILRGVDIFDCVLPTRLARH
                     GSAMSFTGRINLNNQQYARDPRPIVEGCDCYTCRHFSRSYIRHLLISKEMLSGTLISI
                     HNIHTLEELVRQSRRAILSGTFGRYAEDFLTAYGSN"
     gene            complement(48457..48855)
                     /locus_tag="PK898_01375"
     CDS             complement(48457..48855)
                     /locus_tag="PK898_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU18714.1"
                     /translation="MLNIGIKGTKTETVTNENTAAHLGSGDLQVYGTPAMIALMESAA
                     LESVQPYLEPGTTTVGVRLDVEHLAPSPLGMKISCESTLIEIDRRMLTFEVSVFDEKG
                     EIGRGIHKRFVVQKDAFLQKALARKANGSS"
     gene            complement(48874..49836)
                     /locus_tag="PK898_01380"
     CDS             complement(48874..49836)
                     /locus_tag="PK898_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HOU18715.1"
                     /translation="MLLLTYILKGETELDIRYAILGLLQNTRLSGYDLKKAMARSPLL
                     YWSGNNNQVYRTLAELEKEDFVVGEVEQKDTLPLKKFYSITENGKKELQRLSRDFPEM
                     PEIRKPFSIQLLFGGDLSKTEAESLLDRYADEIEGTIAMLSDETPLADPTPYQAILSK
                     LTTDNIRRFYESELLWVEKVRSEALPLLPDESDACRKKDYDVKMETIKVQKNGKDYVK
                     VISGKINEEKDGLALVSACIENDTNRLLLPPEALSDAFYDLSTQLTGLVLQKLVNYRI
                     RAAAVIESAKVRGKFKDFMIETNRGQMFRLFEDFKQAEDWLLKD"
     gene            50059..51681
                     /locus_tag="PK898_01385"
     CDS             50059..51681
                     /locus_tag="PK898_01385"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOU18716.1"
                     /translation="MTKYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFIDEDLNKYSNLTTGKVYWNVLNKE
                     RNGEYLGETIQVIPHITNEIKSFVYSVGQKTNADVVITEIGGTAGDIESQPFLEAIRQ
                     IGLEVGQENCLFIHVTLVPYLKSSGEQKSKPTQHSVRELQSMGIFPKIIVLRCDEPIE
                     PGIVQKISLFCNVKPDCVIENQTIPVLYEAPIMLEKSHFSEIVCRELNINAPDINLTE
                     WNKMLDRIRLCDKVVKIGLVGKYVKLHDAYLSVMEALQHAGYELGTKIKIKWIDSETL
                     KPGNYEKILSDCAGIIIPGGFGNRGIEGKILAARYARENNVPYLGLCLGMQVAVIEFA
                     RNVCGLQGANSREFDEKAEHLVIDFMPDQNDTIAKGGTMRLGSYPCKIVPGSIMEKCY
                     QTDMIAERHRHRYEFNNQYREILTNNGMIISGTSPDGTIVETVEIPKNDFFVGVQFHP
                     EFKSRPNRAHPLFKGFVAAALKKTEKMNESLE"
     gene            51818..53455
                     /gene="hcp"
                     /locus_tag="PK898_01390"
                     /gene_synonym="priS"
     CDS             51818..53455
                     /gene="hcp"
                     /locus_tag="PK898_01390"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOU18717.1"
                     /translation="MENTMFCFQCQETAGNKGCTRTGVCGKSADTANLQDLLRYVTIG
                     LSVVTTRLRSEGKKVDKEANHQVTTNLFTTITNANFDDEAIYERIEKTLKMKENLSAK
                     LSDRQNLPDAATWQSHDRSEMAEKAKTAGVLRTADEDVRSLRELILYGLKGLSAYVKH
                     ANVLKHEDESIDAFIQSALAKTLDDSLSVNDLVALTLETGSYGVKGMALLDAANTETY
                     GNPEITKVNLGVRKNPGILISGHDLRDLEMLLEQTKGTGVDVYTHSEMLPAQYYPFFK
                     KYDHFAGNYGNAWWKQGTEFESFNGPILMTTNCIIPVKDSYKDRLFTTGATGYPGCKH
                     IPGEIGEEKDFSEIVALAKTCPAPVEIESGEIIGGFAHNQVEALADKIVDAVKSGAIK
                     KFIVMSGCDGRMKSRSYYTEFAEALPKDTVILTSGCAKYRYLKLPLGDINGIPRVLDA
                     GQCNDSYSWAVIALKLKEIFGAADINDLPIAFNIAWYEQKAVIVLLALLSLGIKNIHL
                     GPTLPAFLSPNVAKVLVENFGIAPISSVEEDVKILCA"
     gene            complement(53520..54737)
                     /locus_tag="PK898_01395"
     CDS             complement(53520..54737)
                     /locus_tag="PK898_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU18718.1"
                     /translation="MATFFLILIYASFISLGLPDSVLGSAWPMMKIELHAPDDFAGYI
                     FMVVAGGTIISSLFSSRFIKRFGTGVVTFVSVLMTATALFLFHFAPSYYWFLIFAIPL
                     GLGAGAVDSGLNEFVAEHYESRHMSWLHSFWGLGAMTGPIIISKLFASGQTWRNGYLI
                     ISIIQFGLAAILFLSLPLWKKVKEREKIQQELNPPIESDLSQTKKSSGVSVLLKHPGV
                     FPVLISIFLYCGVEQTMMLWGATYLIASRNVTPATAAAWISLFLAGITIGRFLSGFAT
                     FKLSNKMMIRLGELIILIGLTLLIFPLPSPFVQAGIFIAGLGCAPIYPCMLHETPVRF
                     GKEHAQEIMGIQMAVAYLGSTLVPPALGAIGTRTTMIMIPFVLFAFTGIMLFSCEEFN
                     RIQVKIDSVMKSS"
     gene            complement(54888..56111)
                     /gene="pepT"
                     /locus_tag="PK898_01400"
     CDS             complement(54888..56111)
                     /gene="pepT"
                     /locus_tag="PK898_01400"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOU18719.1"
                     /translation="MKTTDRFLEYVKINTASDPDSTGTPSTKIQFHLAESLEKEMNAL
                     GLTEVTLSDQCVLFGSLPATPGYEKVPVIGFIAHMDTSPDFSGEGVNPQIIPDYDGKN
                     VPLGKSGKVLSVKNFPRLKKLKGCTLITTDGTTLLGADDKAGIAEIMTACEEIIEKKI
                     PHGKIRIGFTPDEEIGRGTENFDIRAFGADYAYTSDGDEEGTINFENFNACAATFEIH
                     GVNVHPGSAKNIMVNAALIACEINQMLPSGEIPSKTENYEGFYHLCDIAGDVEKATLK
                     YIVRDHSKEIFHARKESLKLIEKLINEKYGKGTVKLTLKDQYENMAAVFQKNPEPLNI
                     AKQAIKNVGLKPVTAPIRGGTDGARLSLLGLPCPNLCTGGYGFHGPYEHITKEGLEIC
                     TRIIIEIVKIAAEKK"
     gene            56410..56754
                     /locus_tag="PK898_01405"
     CDS             56410..56754
                     /locus_tag="PK898_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOU18720.1"
                     /translation="MAGIRIEIMRKEEKMFIHIVTWKLKENAEGRSKEENIRLILGKI
                     NTLPALIPQVKSLKAGADVLHTPASYDFGLVGEFENQADFLIYRDHPEHIKVAQLISN
                     LTTDRVSLDFVV"
     gene            complement(56759..57208)
                     /locus_tag="PK898_01410"
     CDS             complement(56759..57208)
                     /locus_tag="PK898_01410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOU18721.1"
                     /translation="MTEQEIKPYYHKAQYYETDQMGIIHNSNYLRWFEECRTDYMDQI
                     GLPYDAMEKAGIISVVLVSHCEYKHPLHYEETAEIRAEIVHFNGYKMTIEYIVKNAET
                     GIICATGYTQHGMLSKDLSKPVHLEKGFPLAYKILSDHCKAQSEKEG"
     gene            complement(57226..57849)
                     /locus_tag="PK898_01415"
     CDS             complement(57226..57849)
                     /locus_tag="PK898_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOU18722.1"
                     /translation="MKTDHSGKHYAESKEAEEILAWAQSSNPGRWIGHSKYTGLACRL
                     IAEHCPDMDPEKAYTTGLLHDIGRYVGVVRGRHQIEGYRICMERGWDANARVCLTHSQ
                     VLKDLAKSLDYWDISEEDFEFFNRYIQGIEFDDYDLLVQLGDCLALPSGFCIIEKRMI
                     DVARRYGINKDSVERWNKIFELKDYFNRKTGSNIYRYLPNIEENTIL"
     gene            complement(57846..58310)
                     /locus_tag="PK898_01420"
     CDS             complement(57846..58310)
                     /locus_tag="PK898_01420"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU18723.1"
                     /translation="MTYRIKAYKRRVQYHETDRMGIMHNSNYYRWFEEARIDYMDQTK
                     YPLKVIEEMGIFLPVAESSAEFLSPAEFAEDFYVVSEMVHFTGYKMTINFKIINANSR
                     SVCTTGRIKYGVISKEGKPILLQRQFPEVYEDWQAVLNLPSVIDEFTDPIRI"
     gene            complement(58551..59732)
                     /locus_tag="PK898_01425"
     CDS             complement(58551..59732)
                     /locus_tag="PK898_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU18724.1"
                     /translation="MNPQPQKFSGYERVSAALHHKEPDHIPFDLGGTEVTGMNIHLYK
                     KLRDSLGLPKIEPKISDVKQQLAWIDEDVQEILEVDVCNVPTDPPLNPGLEKPVTREG
                     RYYTRTDEWGIQWSMPVENGHYFDMVKHPLGKAHTIADLEAFRWPDPADEGRFATIRE
                     RNDKIILQDKKASVIARDCAGIWEMALWTNGFEKFFMDMISEKEYAHAVMRKITDYKL
                     AFWEKALEKLGENVLIVSEADDLATQNGPLISPRLYKEMIHPYHKELYQFIHSKAKNE
                     IFIFYHTCGSCKAFIPFLIEEGVNILNPVQVSAKDMGTAELKREFGKDLTFWGGGVDT
                     QKILPFGTPDQVREEVKHRIDDLAKDGGFVFAAVHNVQSDVPVENFMAMWETLRQYGK
                     Y"
     gene            60192..61184
                     /locus_tag="PK898_01430"
     CDS             60192..61184
                     /locus_tag="PK898_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU18725.1"
                     /translation="MKKLSVFTFVVLLVVLMISSVTVFAKDDYTIAVVPKMTSIAWFQ
                     RMEVGVNDYVKETGVDAFYTGPSEGDGALQAQVIEDLIAQGVDAICVVPFSTEALEPV
                     LKKAREAGIVVISHEAAGMENIDYDIEAFDNSAYGRHFMERIGELTGGEGEYVLEVGS
                     LTSASHNQWVDSAKALQEEKFPKMKQYGDKIETKDDQSISYEKIKEVLTANPNIAAIQ
                     GSAMADVAGAALAVEELGLAGKVKIVGTSLVSVSGKYVREGTIDMISFWDPALAGKAM
                     IALAQKVLDGEKIESGLDLGIEGYSNLTLDGKVLTGQAWVDVTKDNVDDPAYNF"
     gene            61295..62779
                     /locus_tag="PK898_01435"
     CDS             61295..62779
                     /locus_tag="PK898_01435"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU18726.1"
                     /translation="MAETFLELKDIHKSFGGVQALKGVDLTIRCGEIHCLAGENGSGK
                     STLIKVISGAHEPTKGEIYIEGERILHLNPIIAIEHGIQVIYQDFAIFPNLTVAENIA
                     INRAVSTGAKLMNWKGARELALEAMEQIGAKMDPDVLVENLSVANRQMVAICRTIIND
                     AKLLILDEPTAALTSKEVQKLYAILRRLRDKGIAVMIVNHKLDEVFEIAERLTILRNG
                     EKISTGMIDEYDRNRFVKELTGRNISESFYEPKVSEEEIFRVENISRKGAFEDVSFSL
                     KRGDVFGITGLLDSGRGQIGDALFGINPIQSGKIFLQGKQIEINSTEDAVRHKIAYVP
                     EDRLTQGLFLDRSIQDNTIAASISKYFIRGKLDEQAMYRATVEWIKRIGCVAPSPEVS
                     VRTLSGGNAQKIVIAKWLNTDPALLILNGPTVGVDIGAKYDIHQILRRLAGEGIGVIV
                     ISDDLPELIQNCNHIMVMKNGRVSEIVEPSEVNETELATMMSSQ"
     gene            62792..63790
                     /locus_tag="PK898_01440"
     CDS             62792..63790
                     /locus_tag="PK898_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18727.1"
                     /translation="MNQKIRNFLTSNEMIVVYIIAGLAILIGSVNHAFNSISTVVNLA
                     RTMLVTLIFAMCEMLVIVSGGIDVSFPAIASLAMIATGNIMLKNGLDNVPLAFLMGAV
                     IGGALGTLNAVLIAVVKMPPLIATLGVSSMTSGATLAIFGAKILTVLPAKLDELYKVS
                     LFTYTNPEGITYSLTVLMLIPIGICLLMWFLLKYTMFGRGIYATGGDIRAARIAGFNV
                     TAIQFFVYIVSGILSGIAGITYMILMRAADPSVLMGSEMMVIAAVVMGGTRITGGHGT
                     VLGTILGVTLISLIKNNLIMLGVPTHFQTFVIGLIMIVGTSITSLKAKHIANSAKI"
     gene            63805..64776
                     /locus_tag="PK898_01445"
     CDS             63805..64776
                     /locus_tag="PK898_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18728.1"
                     /translation="MQKITNYFKKADRIALALLIGSVLIFVLMGVTHPKFFGKSNLQS
                     MLIQFPEYGILAFGMMIAMIAGGIDLSLVGIANLSGIVAAVLMLRMGGTNLSIGIGIV
                     AAVLVGGLCGLFNGFIIGFLDIPAMLVTLCGLQLYSGLALIITKGPAITGLPETFSNI
                     ANGKLLGVPIGAIVFVLIAILLCYLLYYTVYGKSLYLMGTNKIASRFSGINNLRITML
                     TYCLSGIFGSFAGILMASHYNSAKSDYGTSYTLLTLLIVVLGGVNPNGGRGKVMGVVM
                     SIILLQLVSSAFNILRVNAFVKTFAWGLILILVTTATYLTAQKEARD"
     gene            64836..65318
                     /locus_tag="PK898_01450"
     CDS             64836..65318
                     /locus_tag="PK898_01450"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOU18729.1"
                     /translation="MRTIHAQKLSKEAFSPYGQYYNLMSPEGYNLDGFFPDRVLMPFP
                     GGIPAGFSPLITEKPAEYIIEKSEYHNYAQEGILPIDSDILVYVAPASNKPVPELTEA
                     FLVPKGTFVALRTGVWHMGPFPVDREIAHILIALPERTYMNDCHVVEYSQDDRIKIEL
                     "
     gene            65354..66370
                     /locus_tag="PK898_01455"
     CDS             65354..66370
                     /locus_tag="PK898_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOU18730.1"
                     /translation="MKKIINTPEKFINEMLEGIYAAHPDMVKFVGDDLHCLVTTKKVP
                     GKVGIATGGGSGHLPLFLGYVGEGMLDGCSIGDVFQSPSAEQMLAVTKEIDSGAGVLY
                     IYGNYGGDILNFDMAAEMADFEENIHVESVVAGDDVASGAPVKPGAKNTRRGVAGIFF
                     VYKCAGAAAAKMLPLEEVKRVAEKVCENVRTMGVALTPCTVPRVGHPSFELAEDEMEI
                     GMGIHGEPGIRRGKLLPADAIVDEMLNQIIDDLPYRAGDEVAVLVNGLGATPLEEQYV
                     MFRRIDKVLSEKGIKIFHSYVGEYATSLEMAGASISLFKLDDELKELLRAPADTPFFK
                     QFSY"
     gene            66388..>66900
                     /locus_tag="PK898_01460"
     CDS             66388..>66900
                     /locus_tag="PK898_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOU18731.1"
                     /translation="MLDANDFKKLMQHWAEVMVANRDYLIELDSHVGDSDLGLTMGDG
                     FTAASRAIADSDETDIGKLAYTAGKAMSTAVPSTMGTLMASGLMSVGKALRGKTELDS
                     QGVADLFQAFFDGVQNRGKAQVGDKTFLDGLYGAVESLKADAAAGVSMIEAAKKAAEA
                     AHRGFLGTKGM"
BASE COUNT        18095 a        14607 c        15682 g        18416 t
ORIGIN      
        1 acgccgagtt ctatttccct caccctaacc ctctcccaaa gagcgagggt tttctcgttg
       61 aaacaaattt ttatatgcga tgttgaaaca gaaggtgttc tcttgctctt cgtttacctg
      121 tgtataatta gaacaaggat aggagtaaaa atggaagccg aataccgcga tttacatgca
      181 gttgttgaat catatgaaaa atacgatgaa gattcccgtt tgtctgcgga taatatccgc
      241 cgtttggagt ttttgactgc cttggtatat atgaataaat acctggaaaa agggaattca
      301 attttagacg ttgccgcagg caccggcgct tatgcgctct attatgccgc attaggcatg
      361 caagtcaccg ctttggatat caccccttcc tatatcgaga tattaaaaaa taaagccgcc
      421 aaccagcatt tgcagatcac ctcctttgta aatgatgcaa gagatttatc gatgttttcc
      481 gataattctt tcgactgtgt attttgcatg ggtccgatct atcatttgcc gaatccggag
      541 gatcgggaaa aagtgatgcg tgaatctctg cgggtcctga aacccggcgg attgttttat
      601 cttgcctata tcaataagca ttttattttc gctttattgg catcccaaaa caaacaatac
      661 ttacaggaaa aatggtatca acgaattatc gagcaaggcg tgatgtattc aacggatgaa
      721 gattgttttt ggacggatac ctggtttacc accccgggag aaatagaaaa attggccgag
      781 cgttaccgtt tgaaaaaatt aaaccatgtc gcccaggacg gtgtgggccg tttgctggcg
      841 gatgaaatca attccatgcg cccggagtac tataagcgct gggcggaata tcacatccgg
      901 acctgcgaag agccgtccat tttagggatc agcaaccacg gtttatatat cggcaggaaa
      961 aattccgatt agaaaatccg ctttcgacaa aggaatggat tgataaagaa tcgcctgaaa
     1021 aaagtcgatt tagccaatcc tgtgatttta taaggaatga aggtcagatc ggatcaaacg
     1081 caaatactct gatctgacct tattgttcct catgatttat ccttttgtca tcaaccgcat
     1141 aaaaggaggg acatccagtt catctttttt gagaaattcg gggatgtctt cctctttgtt
     1201 ggaagaaaac ggaatctttt tatctttttt ctctgtcaaa ggcgtggcat tggtgcggac
     1261 tgaatccgaa ggacgtctat taaacaggct ttcactgtag ctgtatttaa cggcggaatt
     1321 cgataaagta gggtttggca acggctgatc atagccggtg gcaactacgg taacacgaat
     1381 ttcttctttc atatccgggt tgataacagc tccgaagata aaattgacat ccggatgagc
     1441 actttcctta atgatggctg ccgcttcgtt gacctcgtac aatgtgagat cttctccgcc
     1501 ggtgacattg aaaagaatgc cgcgcgcacc gtcgatcgta atatcgagca acttgctgga
     1561 tatagccatt tcagcggctg ctcttgctcg atcgtcacct tttccggttc cgattgccat
     1621 taacgctgat ccgccttcgg acataatcgc tttgacatcg gcaaaatcca gatttaccag
     1681 ccccggaatt gtgatcaatt cggaaatacc ttggataccc tgacgaagga catcatctgc
     1741 ctgtgaaaaa gcgtgtttca gagagacttt tttatcgacg atctgcaata atcggtcgtt
     1801 ggggatcaca attaaggtgt ccgcatgttc tcgcagattg gcgattcctt ccaaagcggc
     1861 tttttctctt cgcggtcctt caaaagaaaa ggggcgggtc acaacaccga ccgtcaaggc
     1921 gttgatttca cggcatattt ctgccacgac cggtgccgca cccgtacccg ttccgccacc
     1981 cataccggcg gtgatgaaga ccatatcggc tccggaaagc gcttctttga tttcatcgcg
     2041 agattcttcg gcggcaacgt ggccgacctc ggggttaccg cccgctccca atcctctggt
     2101 ggctttatcc ccgatacgga cccgttggga tgctttggaa gccaacaaag cctgagaatc
     2161 cgtattgatc gcgataaact ccaaattttg cattccctcc tcgatcatgc gattaatcgc
     2221 attgcatccc gccccgccga cgccgacaac cttaattctt gcaaaacttt caccgtaatt
     2281 ttgataattg ttttccattt cttcctcttc tttccgttat tgacaatacc attgcatagt
     2341 attctgacat cagatgatct tctcaggact ttgtcctatt ctttccacgg cataaaaaat
     2401 tttagtagcg gtcggttttg tccctcctta tcaaggcaaa agatatcgtg caaatttttt
     2461 gattttatcc atcgtgtcgc tgacatcttt cttcttaggc tgccctccca ccgtttcctg
     2521 catcatcatc gcccagtaaa gtagcccgac actcgttgaa aaagccggac tgtccaactg
     2581 gtcgatcata ccggtaatgt tttccggttt tgccaaccgc accggcatat gaagttcatc
     2641 ggaagccaac tgatcgatcc ccggtaaaag gctggttccg ccggtgagga ccattcccgc
     2701 cggcatgagg ttgtcataac ccgatcgttt gatttcctgc agaaccagtt gaaagatctc
     2761 ttgagtccgc atctgtataa ttttcgaaag ttcttttcgg ctgaattttg ccgcttcatc
     2821 ttgaccgaag gttcgacagt aaaaatattc ttcatccccg atgaaatccg gaatgcaatg
     2881 cccatggatt ttcttgacct cttccgcttg agataacggt aagtgaaaag cctgagcgat
     2941 gtcattggta atatgaccgc ctccgacggg gagaatattg gaatgccaca catctccatt
     3001 gatgtaaata gctaaaccgg tcgttccacc gccgatatca cagaccacca ctcccatctg
     3061 tttttcgata tccgttaaaa tcgcttcgcc gtccgccaac ggattcaaaa cccattgatt
     3121 gacgtcaatt tcggctgtca taatacattg acggagattt tcgatagatg atgcggaagc
     3181 ggtgataatg tgcgcttcca gatccagtct tgagccgtac ataccgatcg gtgagcggat
     3241 tccgtcaacg ccatctacac tgaaagttcg ttgcagcaga tgaataatga cacggttatg
     3301 cgggatcgga atagcccgga ccgcttcaac gacacgtgcc acatcgtatt gatcgattac
     3361 tccgccgacg acacccacag tcgctttgtt attaattgaa ctgatatgtg ttcccgcgac
     3421 actgacgatg gcattggtga tttccaaccc cgaagttcgc tcggcctttt ttaccgattt
     3481 cgcgattgaa ttggcagctt gaggaatatc gaccaccgtt ccctttcgaa tgccttcgga
     3541 aggttcaata ccgactccca aaatacggat gctgttttca tcttcaattt gagcaattag
     3601 ggtgcaaatt ttagtcgacc cgacatctat cgctacaaca atcgattttt ccattttttt
     3661 actccgcgta ccgataataa ggggcttgcg tgaattccaa gctcagcata tccggttgta
     3721 tttcgttttc tttaaaatat ttttcgatac tttccgccat tttcattttt tcagttaatt
     3781 ccgaatcatc gattccgacc cacagcgtgt agccgtaatc ggtttcccat ccgtaaccgt
     3841 tgtcataggt gtaatacaac ttcttccctt gcggttttac ctgattgatt gcaataacgc
     3901 tttgcatcat ttttttactg aatttttccg gaaatttgcg gtcattttta tttttcgctc
     3961 cggggaaact gttggacagc acataaatta aatccgatga agcggaaggc gattctttca
     4021 agattgtccc gtcttcgtcg atccaaatct tactgccgcc gaaatcccat tcgatgaccg
     4081 gaatgcgttt caacatctcg attctgacgg tttgaggtaa ttccaccgtc acattcatct
     4141 cgcgaatttc gggatatcgc tttaaaagca cttgacggat ctgagcggga tttgacatat
     4201 aaatagggat atctcgtaaa ccggtgtaat agatgatttc ttccatattc acctgttcga
     4261 tcccgctgac ttccggggca atcacccgaa attgcggaag aaatgaaaag gcggcagccg
     4321 cacaactcaa caggacagtt aagacggcag aaacaatcct cgcatttttg cgctttttcc
     4381 gggagggatt tttcacaccg cttacgatag gcgtcccata tcttttatgg gattgcatcg
     4441 tcagaagcga tatctccggc tgctcatctt ttttatcagt tcctttttta aaaatacttg
     4501 ccatttcctt ttctctgcgg tcttaattca ttatttcaat ttcggttgtc agatcaatcg
     4561 aaaatctctc ccatacggca tcatgcaccc ggtgaataag ttcgataaat tcactgcttt
     4621 ttactccggg ataagtaatg aaaatatttc cgtgatcatc cattactttt acatttccga
     4681 agcgtttgcc gttgaatccg cattcggtaa ttaatttacc ggcataattc ccctgaggat
     4741 ttcgaaagac ggatccgagg gaaccgtttc catgaaaatt gaatttctgt cttttttctg
     4801 cgatgactgc cattttttca tggatttcat cgtagttccc tcttgataat tcaaaagttc
     4861 cgctcagaat agtccctttc aattctcctg acttaaattt actggtgcga tatccgaaac
     4921 cgagatcttc gtttcgcaga tcgacgatct cattattgtc ggttaagaat tgcacggatc
     4981 gaagaatgtg ggaaatatct ccgccgtaag ctccggcgtt tccgtaaatc gctccaccaa
     5041 ctgttcccgg aatcaagctc gcccactcgg cacccgtcaa agattcttcg gcgcaagctt
     5101 tcacaaatcg gtttaactct gctcccgaat caacggtaac gagtactttt tcctcttgtg
     5161 gttcgaaaga tatttcatcc gattgattga taaccaacaa tccgtcaaat ccgttgtcgc
     5221 tgaaaagaat gttggataat cctcctacga ctttgaaagg ttttccggaa gattgatatt
     5281 ctttgataat cgaaaccaaa tcttcttttg aacgaacaat taccaactct ttggccggtc
     5341 ccccgcacat tgcttttgag tattttgcga tcggttcatt ttttaaaata acgccgtaag
     5401 gctttttaac ggaatcgttg gcagcggctc tcaaccgttc gagggctttc ggtccgattt
     5461 ggtttgcatc tcccgcggaa agtgttatca ccacatcgcc gggttgcaat tcgttcatca
     5521 aaatctcgac agcctgttca tttgtttcgg caaacaagcc atccggatag gcatttatca
     5581 tggctttttt cagatcctca aaaccgaaat cggtttgggt ttcacgagag gcatagatat
     5641 tcgtaattaa aagtttgtca gccagatcaa aagcttttat aaacttcggc aataatgtct
     5701 gagtacggct gaaagtgtgc ggctgccata gtgcccaaat tcttcgatta gggaatgcat
     5761 ctttcgccgc gctcagggtg gcttgaatct cagtcggatg atgagcataa tcatcaacaa
     5821 ttgtgatatc attccaagct gcgctgattt caaatctgcg ggctattccg ttgaatgctt
     5881 taagtgcttc ggataccttg cttaggtcta aatttttcaa tgcacagcat gccgttgccg
     5941 ccgtcgcatt gaaaacatta tgttttcccg gtattcccag ttgaataggg agtttccgat
     6001 ttttcgcgga atcaagaaaa ttgaatttat aacagccgtc aagaccgatc tcaagatcgg
     6061 tcgcgcggaa atccgccgat atggattcac cgtaggtata aaccgaaaca tcggcgggca
     6121 ttttgtcttt taataattgc tggttcggat catcggcatt catcagaata attccgccgg
     6181 gcttgacttg agagataaat tttcggaagg cattcaaata cagatccata gtcgggaagc
     6241 aatccggatg gtcatgttcg acgcgggtta atattgcgac ggaaggaaag agcccgagga
     6301 acatattgtc gtattcgtcg gcttcgatca caaattcttt gctcttcccc aaagccgcat
     6361 tcttccccag atttttcggg gtgcttccga taataaatcc gggttctgat cccgattcgc
     6421 tgaacagcca agcgatcata gcggtggtgg ttgttttccc gtgtgtcccg gcaacagcga
     6481 tcacatcccg atcctccaac agatcattta agaattcgat acgacgtttg acggggatac
     6541 ctcttcgttg cgcttcccgc agttccgggt tatccttggg aatcgcggaa gaatagatga
     6601 cgagatcgac attcccgata ttttccgacc ggtgtccgag aaaaacttta attccggcat
     6661 cttccaattt cgacacaata tcggaacgag aacggtcgga gccggtgaca attgctccgg
     6721 cttctgccag taccaatgcg atggctgaca ttccggttcc gccgatgccg ataatatgaa
     6781 tcttcatccc gtcatgtatt ttcatcttca actctgttca ctctttgata tggccaattc
     6841 ttccagcaaa acagcgatat tttttgcagc atccggaatc gccaaactgc tcattgcttt
     6901 tttcatttcc gctaatttat cccgattttg catcaagtct ccgacggtac cgacgatttc
     6961 cttatccagc atggtatctt caattattat tgccgcccct ttttccgcta aataatcggc
     7021 gttcactttt tgataacgcc aagcataggg gtaggggact aaaatcgccg gcaatccaaa
     7081 caaggggaat tctcccaaag tggaagcgcc cgcacgggaa atgaccaaat cggctgcggc
     7141 aaacgcggct cccatttctt catgcaggta tggattgatg tggtagtcct ttgcttctgc
     7201 cggatccaga tgttcacgaa ccttacaaac atagtcccaa tccgattggc cggtgatatg
     7261 gataatctgg cataagttca gcagttcatg aatatggttt gcgaccgcct gattgataga
     7321 gcgcgctcct ttgcttccgc cgaaaatgaa caaaaccggt ttcagatcgg acagaccgaa
     7381 caaattcctg gcgttttcct tcgtccattt cctcaactcg gctcgagtcg gatatcccgt
     7441 aaccgcaatc tttttatggg aaggcaaata tttttttgaa tcgtcgcaca ccactgcgat
     7501 cgtatcagcc agtcttgcca gaactttcaa tgacaaaccg ggttcaatat ccggaataaa
     7561 aatcagctgc ggaatatgaa ttccggcgaa agccatcgga atgccgatat aaccgccggt
     7621 aaagaagagt acttcgggtt taaaatcttt cagaatccgg cgggaatcga agaaaccacg
     7681 gaccagactg gctatatttt tcggcagctt tttccagccg acaccatgta ttccggcggc
     7741 ggcaattccg cggaattcca atccggcgtg ttttacgagt gaatattcca tacctttttc
     7801 ggatccgacc cagagaatgg cgtcgctatg gtttttatct ctttcttgaa ccacggccaa
     7861 ggctggatat acgccacccc ccgttccccc cgcgcagatc aataagcgca tccggtgttt
     7921 tcctttcttt tttcaaagct tcgatggctg acgatctgga aacactcatc agtattccga
     7981 ttcccgccaa cgtacagata agacttgatc cgccgctgct gatcagcggc aatgcattcc
     8041 ctgcgaaggg aacgatattc accatgactc cgatattaat aaaggcttca ataccgatcc
     8101 aaagcgttac tcccgctgcc agtaatttcc cgaacgtatc cggtgcattt acggatattt
     8161 cataaccgcg ccaaatcaag actaaataca gaagaatgat aaaagtgccg cccaataagc
     8221 cggtttcttc caaaattacg gtgaagatac tgtccgtcca agccaccggt aatccggtca
     8281 gtttcacgac tcccttccct aaaccgactc cggttaaacc tcccctcagg atggcttcgg
     8341 tcgatcgttg aatatgataa gaagccaaac tcggatcttt cagcccgttc acataatcat
     8401 tgatgcgcgt actgacttta tcaaaaagga agtaaccgca taagaatata aaaagggcaa
     8461 gcagcagaat cactcccaaa tgtttggggt tccctcccgc gacataaaac ataaccgcac
     8521 cgaggaaaat aatcgtgatt gttgcgctga catccggttg taagagaacg agtaccgaaa
     8581 taatcccaat gatggagata accggaaaaa atccccaaac aagcgtgctc agtttatcct
     8641 gcttattgct taaccagtat gcaagataaa taatcagagc gatttttgcc agttcggaag
     8701 gttgaacaga gccgtttaac acggtgcggg tagcaccctg aatactggac gggataaaaa
     8761 gaaccaacgt cagcagaaat attgtcccca gaataatcag ctgggcaata tttctaataa
     8821 aatggtaatc catgaaggtc agaatggtgg ccagaaccaa tcccaatacc acccaaattg
     8881 cctgtcggat gacgatataa ccggcattat catattcctg caatgcaaag ttccaactcg
     8941 ctgaatacag aaataacaag cccagaatca ataaaataat aaccgttacc aaaatcggaa
     9001 cgtcgatcag cagcttggtt gagacacgag cttgcttggt ttttagaaat tctacgtcag
     9061 ccgcattacc cattcggtaa aagcctttcc tctttcttca aaatcacgga aagcgtcata
     9121 actggtccct ccgggagaca gaaggacaac atccccttcc cgacaaacag atgcggcagc
     9181 atctaccgct tcttcgagcg aatcgacctg aataatctcc aattccgact cggaagtctt
     9241 gttccgttcc aacgcctttt tgattaaagg tgccgcttct ccgaacaata tcgccgcatg
     9301 tgcatgatga tgaatttcgg ttgccaattc gtcccatggt aattttttat ctcgtccccc
     9361 caaaagcatc accaatggtt catcaaaact tctcaccgca gccaaagttc gttccggtgc
     9421 ggtagcgatg gaatcgttat accaatcggc tccgtgaaaa ttccgtacgt attgcaaacg
     9481 atgttccacg ccggtaaagt ttacgacggc ggaacgcatc gcttcgatac cgaatccggc
     9541 tgcggtagat acggcaaaag ccgccattgt attggcaata ttgtgccttc cccgcaatcg
     9601 aatttgatcc aagcggatca gattttcaat ttcaccgttc atctctcgtt tgatcgccat
     9661 attttcgcaa tagataaccg gatcgccatt gttttctgcc ggtttatcaa atccgaagct
     9721 gatgattttt cctttcagat catttcggag atgccaagag ttttcatcgt cacggtttag
     9781 tacggcgata tcatcagccg actgataatt taggatgttt ttcttcgccc ggatataagc
     9841 ttccatcgtc tcatggcgat cgaggtgatt cggtgtgata ttcaatactg cggcgatatg
     9901 aggactgatg gtagtcaatt ccagttgaaa gctggacagc tcaagaatga cgatatcgtc
     9961 gggttgaatt ttgtcggtca ttgttaacaa aggcattccg atatttcctc cgacccacac
    10021 tttttgatca ggtttttttg ccgcttcggc gattcttccg accagtgtag ttgttgtggt
    10081 ctttccggag gaaccggtga tgccgatcac cggggcactc acttccgtta agaaaatctg
    10141 tgtatcgttc aaaacggcga gtcctcgttc gaacgcttcc tttaaaatgg gttgttttaa
    10201 atcagctccg ccggttacac agacaaaatc ggtttcatct aagagacttt gcggatgtcc
    10261 gccggtaacc caacggaccg gcaacatttt taaggattcc caagcggaac ggagctgatc
    10321 aaaaggttga atatcgctga tcgtaacaat cgctttccga tcggccataa aacgggctaa
    10381 tgcttgaccc tgacggcctg ctccgagcat taaaatcctt tttccttgcc aactgttttc
    10441 catttttatg tcttttcgaa ccctttatgt tttctcgatt tctgtcagat catagcgatc
    10501 gcgattccca acaagactcc caacaagtta atcaaccaaa agcgttgaac gacctgaact
    10561 tcactccagc ctaacatttc aaagtgatgg tgaagcggtg ccattcggaa aaagcgtttc
    10621 ccatgagttt tcttaaaata aacgacctga atgatatcgg atactgcttc gctgaccgga
    10681 ataattgcga tgatcggcag aaccagaatg tgtccggtaa tcaacgccac gaccgccaga
    10741 gtcgcgccga gggataaaga tccgcaatca cccatgaata gttcagccgg attaacgtta
    10801 aaccataaga atccgaataa tgctcccacg agaatgaagc agaacctgcc gatgtaagcc
    10861 tgtcccacca tcagggcaag gacaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnngaat gaagcagaac ctgccgatgt aagcctgtcc caccatcagg gcaaggacac
    11041 catagcctgc gaatgcggta gcggcgatca acccggccag cccatccagt ccgtccgtca
    11101 ggttaacggc attggacatc ccgacaatca gaaaaactgc aatcgggata aagaaatttc
    11161 ccagatcaat cggttcagaa aaggcaggcc aatagaaatg agaaacaccc agaaaatgct
    11221 tcatcatcca agctatgacg atcgcaaaaa ttacctgaag gataaatttt gttcgggcgc
    11281 gcatacccaa gccccggcgc ggaccgcgaa tgccttccca gtcatcgatc gctcccaaaa
    11341 gaccgaaaac aatcatggta agcatcggca agacaaaaga ctgcccgata atttggactc
    11401 cgaataagga agcggcattc aagagaacgg taatcaataa tgttgggata atgaacatga
    11461 ctccgcccat ggttggggtg ccgatctttg caaaatgagt atcgggacca tcaattcgta
    11521 ccaatttccc gatgttgaat cggtgcagca attgaatcaa cggtttcccc caaatgaagg
    11581 tcatcataaa accgagaata gacagggcaa gtgttaatga ggaatctttc atattattct
    11641 ctccattgct tcaacgattc gttccatttg cattcctctg gatcctttaa tcagaaccac
    11701 ttcgcctttc ttgaaaccgc gttgccgagt atattcgatt gtttcctgaa cggtagcaaa
    11761 ccatttgact ctttggggat ccattccccc ttttaaagcg ctggaaacca ttttctttgt
    11821 tttttcgccg accgcgatca gttcatcaca aacctcggct gcccgcgctc cgactaatat
    11881 atggccgttt tcttcatatt gtcccaattc acgcatctcc cccaaaatgg caattttata
    11941 tccgtccatt tcctgcagca ggtttaatgc agccatgacc gattcgggag atgcgttgta
    12001 agtatcgtca atgatcatgg caccgtcttc cgaatgcatg accgccattc gaagctgcga
    12061 ttgtccacga tttaatccca taaaaatatc ttcccaactg attcccatta tcttccctac
    12121 cgcagccgcc cggagagcgg tatgaaccga atgtctgccg atcagaggaa tgcgcacaaa
    12181 gaaactttcg ttctgataat gaaagcagaa acggattcct tccaatccat gactctcgat
    12241 ttgatccgcc cacaggtccg ccttcgggtc cagcccgtaa taaaaaacat gcgcggacgt
    12301 tttttcggcc atcggtctga cccaggggtc gtcataattg aggatcgcat aaccgtcttt
    12361 cggcaaagcc tgaaccagtt cgcttttccc ggcagcaatc acttcgatgg atccggcccg
    12421 ttccgcatga acggttccga catttgtgat cacaccgatt tcgggttttg aaatatcgca
    12481 gagcagtttt atttccccgg gaacatagaa acccatctcc aaaaccgcga cttgatgacc
    12541 ttttccggcg cgcagaagtg tcagcggcag accgatttcg ttattgagat tgccggtatt
    12601 tttcaaggtg tggtattttt ggcttaaaac ttccgcaatt aattcttttg tggtggattt
    12661 tccgacggta ccggtaattc cgattacttt cagatttaac cgatcccgat gaaaagcggc
    12721 tgttttttgc agcgctgcga ctgtgttgtc aacgcgcagg caaaacggga atggcggaaa
    12781 agattccgga agtttttccg tccgtaaatc aacggtcgta aatttatccg aagtatcttc
    12841 ctgcacaagt gccgccaatg cccctcttct gaaagcttcc ccgataaatt gatgcccgtc
    12901 tgtattttca ccggcgattg ccacgaacat tgacccgctg atcgcctggc gactgtcaac
    12961 aaccgcttcg ctgaaaaaac ggtttttttc tgccaactgt tgtccggtta atgcttccaa
    13021 gatttcattg agtgaaaaca ctttattccc ttatccaatt cccgggttat cccgtttgtc
    13081 tgttatcggc cgaaccccca atcctttcgg taactgcgaa atcatcaata atactcattt
    13141 ttcggtactt cggtcggaca ggtttttgaa ttcaaacatg cccgttggcg atcgtaagga
    13201 atatgcaaat acggcattat ttcattcatg acatcactga ataaaggcgc cgaaacttcg
    13261 gatccccaaa tactgatcaa aggttcctgc agccatacgt aagcgatgaa ttgcgggtca
    13321 tccgtcggac cccagccgac gaaagatgca ttagtgacat tcaggacgta cccatagccc
    13381 tctttcgcaa tctctccggt tccggttttt cccgcaatct ttactccatc gaggagagcg
    13441 tcggaagctt ccacttccag agatgtctca agcatatccg taaccgtatg cgccgtttcg
    13501 gcagaaatcg gtcgccccac tacttcaggt tgaatcactt cggtatgatc gttgtaataa
    13561 atcgctttga caatatgcgg tttcatcatt attccgtcgt tcgcaatcgc actcactgcc
    13621 tggatcatct gaatcggagt cgtcatcaaa ccctgaccga atgagttggt cggaaggctg
    13681 atctccgtcc agccctcgtc tccgggttgg agaatcggat aataatcttc cagtgcgagt
    13741 tcgatgccgg taggtttatc cagatgaaac ttgcgaagat agtcataaaa aaccggagcg
    13801 gtgatttgct cggcaatcca tgataaacac gtgtttaatg aatgttgcat acagccgatc
    13861 attgtttgcg gaccccaagc gccccgatcc cagttataaa tatcaatccc catcacattg
    13921 aaggtaccgg tatcgttgaa aacggtttcc ggtttgatga cacctgagtc aatcccgatg
    13981 gctaatgtaa taaccttaaa taccgagccc acctcatacg gctgcatcac cgccgggttg
    14041 aatttatttt ctctcgtaaa agttgtggcg gattcccagt aatgattgag attgatccgc
    14101 ggataggtcg ccattgccag gatttcaccg ttcttgggat tggcgataat aatcgttccg
    14161 ctctttgctt tttgttgctc caccgccaac ttaacttgct cttctactaa tttctgaact
    14221 ttacgatcga tcgtcaatgc gatcgatgcg cctttgggca gatccggaat gctttccgga
    14281 acattgggat ccaaagagaa ttttttctga atggtttcac cggataaaat atcgtcgtaa
    14341 tattgttcga tgccgaaaga agcgccgctg gcgggattca aatacgggta aaaaccgata
    14401 atattggatg ccaaatctcc gtccgggtaa tagcgataaa tattcggata atacaccacc
    14461 gcattcaaat tttcgaaaac acgtgtttta ctctcgaagc tggttgtatt ccgctcattc
    14521 agagattttt ttgccaattc gattttatcc acttgttctt tggtagcaaa attggtaagc
    14581 ggaacttcaa ccgcttcatt ctcgacatac ggtgtttcag cagcctgaat aatcttttcc
    14641 cgatcgatac cgagaatcgg cgcaagatag tttgcgataa attcaccgtg cgtattagaa
    14701 gcttgtaatt ttagtgaaat cgtgtaagaa atggaatttc cagctaacaa ataaccttcc
    14761 cgatcgtaga tattaccacg ttcggagctt actgacaacg tcgtatattc gtatgccttg
    14821 ccgatcgttt cctttaattc aactgcactg gcattcgtct gaagaaataa tatacgcccg
    14881 attaccaaaa ccgcaaaaat acttaaacaa atcgctaatt ttttaaattt ggagtcgata
    14941 ttcgagttca tttttcactc tcctttgtcg gcaatttccg gaatatatat tgctgaaggc
    15001 tttcggtata ttcgggcttc agcggagata ccggcaacgg aggaagctga actcttcttt
    15061 ccgaaagaat gtcgatatcg ggtatatatc cgggaatagg gagataggta taaatgtctt
    15121 catcatcgaa atcaattttg atatagcctg cttgggtggc ttttttctcc atcaatgcat
    15181 aagaagtgag gattccttcc cgagtcacat aatccgcaat ggttcgggtg agttcgtttc
    15241 tttggtcatg taatatttgg atttgcagtt tcatatccgt catttgtgcc gaataattta
    15301 aataaaccga tgcaatgcag gcacccaaaa ccaacaccaa caggatattg atgatggatt
    15361 tcagctgttt tcgccaaggt gccaatcgat atgcatgctg ttcggttttc atttttctga
    15421 ctcgattttt tcgaccactc ttaattttgc acttcgcgct ctcgggtttt cacggatttc
    15481 gtcatctttt gcagtaatcg catgacgatt gatccgtttt aaactcgcct tatgaccgca
    15541 ggtacaaacc acttgttcag gcggacagag acagtccgta ctttcgatgc ggaaaaagtt
    15601 ttttacgatc cgatcttcca aagaatgaaa agaaatgacc gctaacctgc cgcccggttt
    15661 caacaatgcg atcaaaacag ggagtgcttt ttcgatggtt tcgagttcgt tatttacggc
    15721 gatacgcagt gcttggaaac tttttgtcgc cggattgttt ttggatttcc gattggtatg
    15781 cacggcattc gcaacgattt ccgcaagcat cgtcgtagtc tcgatcggtt ctcttacgcg
    15841 tgcactgcaa attgccgcgg cgattcttct tgcggctctt tcttcgccgt attcccaaaa
    15901 aatacgtgaa agttcttctt cggatgcgaa attgactaaa tcgtaagcag aggtcttctg
    15961 attcggatca aaccgcatat ccagcggtcc ttccttttga aaagaaaatc ctttttccgc
    16021 tgcgtccact tgcatcgaag aaatgcccaa atcaagcaag attccgtcga accccgttat
    16081 gttatcgaaa caggacgtga tatatcggtc cgcaaacgca taggatcccc ggataatttt
    16141 gacccgttct ccgaatttgg ccaatcgttt tgccgctatt tcgatcgcgt ttggatctaa
    16201 gtccattccg atcagctttc ccgacggagc ggatgcgttt aatatcgctt cgctatgccc
    16261 tcccgccccg agtgttccgt ccagatacca ttttcccggt tccggtcgca gtgcctcaac
    16321 gacttcgttg agtaaaacgg gttggtggct taaagtttcc tgcgtcatat tttgttactc
    16381 atcagacggg caaattcagt cgttaccgaa tcgtcatgga aaacttcttc ttcggctttc
    16441 ttccattcat ctaaagtcca gatctcgata tattcgtccg agccggcaaa aatgattttg
    16501 tcgccatcct ggaaattaaa ttcatcccgt aaaaatggag gaatcagaaa tcttccgttc
    16561 gcgtcaaatt cgattggttc cgctttattg aacattcgcc ggcgatattt tcgggcgacc
    16621 ggatcggcca aattaaatga actgatttca ttcgccaact tttcaaagta aggcaaaggg
    16681 taaatgacca gatttttatc gattcccacg gaaatataag caccctctgc cagcaaattt
    16741 ctaaatcgtg ccggcagaca ggttctgcct tttttatcga ccgtgtgttc atgtattccg
    16801 gtgaacatat tttctatttt gtcctttaaa aacagaagac tggaaaacca gtctgccact
    16861 ttatgggaat ctataccact ttatgttcca ttataacaac ctggcttaaa aattgcaata
    16921 gataaatccg gattttctaa ccaattttca aaattggttt atttttggtt taattaccgg
    16981 tccgattttt gggtgtaatg tgtttttttc cttctttcgt ttcaattgtt ccgataaccc
    17041 gcgataaggt gtaagggttc aatcgcaaaa taatcggaaa accgcccttt cggtatttga
    17101 aaatcgaaag ttataataaa cggggatttc ctttcagatc ggattttccc tctattatgt
    17161 ctcactgatc ggaaaacttc ttggagcatg aaatattttg caaaatgatt ataaggaagg
    17221 gcttatggag tcggagtatg aagttcatca taatgatcgt gaattattaa ttaaaatcgc
    17281 tcgtgaaacg atggaggaaa acgatttgct caccgagttc ccgcctgaag tgatcgctga
    17341 attggaacaa atcaatgaac ctgccaaacc ggaaggcctt ccgaatattc atgatttacg
    17401 acacttttta tgggcatcaa tcgataatga tgattcgaaa gatttggatc aaatcaccgt
    17461 ggcggaagcg ctttcggacg ataaagtacg aatttatgtg gcaatcgcgg atgtggacag
    17521 ccttgtcaag aaagattcgg caatcgatcg atacgctcga tataataccg ctacggtgta
    17581 tcatcccgca attaatttcc ccatgctgcc ggaaaagctt tcgacgaatc tgacttccct
    17641 caatccgaac gaagatcgga tggctgttgt atgcgaattt acggttgatt ccgaagggac
    17701 ggtttgtgaa ccgaagattt atcgggctta tgttcggaat catgctcaat tggcatacaa
    17761 cagtgtcgcc gcctggcttg aagggcaagc ggagatgccg agtgcattgg caaccgttga
    17821 aggattggct gagaatattc gtctccagaa tgaagttgct caaaaattac gaaagaaacg
    17881 atatcttcac ggggcattga ctttacaaac aattcaaaca attcccattt ttgacggtga
    17941 tgaggtcgtg gatttggcta tcgataaacc gaatcttgcc aaggaactca tcgaagattt
    18001 catgatcgca gcgaacagtg tttcggcggg ttttttggat gaatgcaaat tcccggctct
    18061 tcgcagagtt gtccgcatcc caaaaaggtg ggatcggatt gttgatttag cggaacaata
    18121 cggatataag ctctcccgga aaccggactc aaaagctttg aatgaatttt taatcaaaga
    18181 acaacagcgt gacccgttaa gatttccgga tttatctcta acggtcatta aactcctcgg
    18241 atccggagaa tatgtcgccg aaaagcccgg agaaaaagcg cccggtcatt tcagcctttc
    18301 ggtaaaggat tacacccatt caaccgctcc caacagaaga tatcccgatc tggttacgca
    18361 aagactgatc aaagcggcgc tgaatcatga agagccgcct tattcttttg aagaactgga
    18421 tgaattagcc aagcatataa cggctcagga agatctggaa gcaaaaatcg agcggaaaac
    18481 ggccaaagcg gctgcggcgt tggttcttga aaatcatatc ggagaaacgt ttaatggatt
    18541 tattaccggt gcttcggaaa aagggtattg ggtacgaatt cttgagatcc ctgtcgaagg
    18601 aaagttgatt tccggttttt ccggtttgga tgtcggggat cgggtgcagg tggtgttgga
    18661 ttcggtggac gtcgaaaaag ggttcatcga tttcaaacgc ctcaaaaaac gatagaagtt
    18721 tttgatgatt ttgaatcaag actttggctg tgagtatttt acagcttccc gaaccatact
    18781 gtgcagggaa tggttatcag gaaaattacc gagttgatag aattcgtgtg attggtaagg
    18841 aaggatgctt catggcacac tttcacgaaa agttgagcag tgacagccta aaggaaagaa
    18901 tgttttgggc gtctatcttg tttttactgc tttttttcgg tgtgacgatc ctgagctttt
    18961 atctttttcc cgaaggactt ctgaaaaata aaaacccgtt acaggcttgg gaaccttcag
    19021 aaagcacctt gatccttacg ttgcagatat tcttttacaa cctgttatct gtgttgatca
    19081 ttgttctcgc cggtctgttc ggtaccaaaa aagagggtga tgcaaactat ttatccatcg
    19141 gttatcaagc cttttatacg ttgatctgta tcaactcgat tgttcttggt acgtggtcat
    19201 tttccatcga aagccaacct gttaatctga cggcaaggct tctgcgaacc tttgatcttg
    19261 tacacagagc aggactctgg gaaatggcgg gtcagctgct gattacttgc tcaaccgcac
    19321 atattgccgt aattctttct tgtaacaaaa atactgtggt aaagaagatc aaagatatcc
    19381 gcctttcgaa atccgaaaag actgttttta tcacagggat tctcctcatg ctgatcggtg
    19441 ccgtggtgga aagtgtagcg atcgggggac aagtatcata gaaagggttc gatctgcctc
    19501 ggtttggcag acaattcgaa cgtgtatcat aggaaacgat gcaaacatag ctgccgccgg
    19561 attttttgac gtcttgcttg tttttcgttc ttccgataag gcttcgcttt ccgaagagat
    19621 gacattatta ccgaataacg acaccagttc gtcacatttc ttgcaaagcg tcatacattg
    19681 tgctaaaata acggcataca gttcgttagg aaataacaga agtgggggag gccccacttc
    19741 ttggtttgtt tggatttaga ttaggagatt taaataggat gaagaacgag tttgttctgg
    19801 cttttaatga aatgcttgac gaaaagcaac tgccgcgcga aaccgtcatc aaagcgttgg
    19861 aatcagcgat ggtttctgcg tatcgcaaag ctgtcacggc atccactgct cagcatattg
    19921 aagcaaaatt agaccctgat acgggcaatt ttaagatata tgccgaaaaa gaagtggttg
    19981 acaatgtact ggatgaccga accgaagttg ttttggcgga agccagaaaa gttaatcccg
    20041 atgcccaaat cggcgatatg gttgtggtgg aaacaactcc caaggatttc ggaaggatcg
    20101 ccgcacaaac cgcacgacag gtgattcaac aaagacttcg cgatgcggaa aggcaagatc
    20161 aaatcgaata ttacgaaaag aaagtcggtg agattgtcag cggcgtcgtt caatctattt
    20221 cgaatgaagg ttatacaatc ggactcgacc ggaaagcgga agggttactc ttgcgaaagg
    20281 atacgatcaa aggggaacat ttccgactac atcagcatgt acgtgccttg attgtcgaag
    20341 tgaaagacac ctcgcgcagt gcacagatta tgctttccag aactgaccgc aagttcctgt
    20401 ttcgccttct tgaaatcgat gttcccgaaa tttatcacgg aatcgtggaa gtccgttcca
    20461 tcgctcgcga acccggaatg cgggcaaaaa tcgccgtttc ggctacccag caggggatcg
    20521 atccggtcgg cgcatgtgtg gggatgcaag ggaaaagaat tcaaccgatt gtgaaggaac
    20581 tccatgacga aaaaattgat gtaatcgaat ggaacccgga tccggctgtt tttatcgcca
    20641 aagctatcag tccggcaaaa gtggtcggtg tatatttggg caataataaa aatgcgacgg
    20701 tcgttgttcc cgaagatcag ctctctttgg cgatcgggcg ggatgggcag aacgcccgtt
    20761 tggctgcgaa gttgaccaat tggcggattg acatcaagag tgtgattgaa tcggccagtg
    20821 aaatattggg tgcgatcgat tccaaaaagg atatcgtcga agagaatcag gaatcgggcg
    20881 aaagaaccgt cattattaac tatgcggatt tgtttgagca agagaaagat aatattgaag
    20941 cgattcgcgc tatcttagcg aagcgagccg agaatcgacc gatttcaccg gatgaaacgg
    21001 aaacgttgaa tcgatttgtg gatcgaattg agagacagaa agtcgccggt tataacctga
    21061 gcatggtctc cgaggtgatc gagaaattaa gcaagcaaaa tctgacggaa gaaaccctgt
    21121 ttgcgatgcc ggtcagtgca agcggattac cgaaatatgt gaccaatatt ctttatgcgg
    21181 gcggatttcg aacattcggt gatttatata atcatatgat gagtaatccc gaagaaattt
    21241 ataaactgca gggagtcact ccgagaatca tgagtgatat cgcatcgctg gtaaaattct
    21301 taggatcgat cggttcgaag aaaacggaaa ccgaaccggt tgaagtttcg gaagaacagc
    21361 caacaatgga agctcagccg gtcgctgaaa ttgccgaagc ggaagagttg cccgagccgg
    21421 tttcttttga aaaatcggaa cgtaagtatg agggaaaagc ggaagatttt gtcgatctga
    21481 ctcaacttcc tcttcaaacg gagaccaagc cggctgaacc cgaacctcag accgaatatg
    21541 aagatatttc ttttgatgag cttttcaatg cgatgcgaaa tgaaatcgcg actcctacgg
    21601 aatatttaga ggatgaagac gagctgaaag acgatctgac ttcaactaag ggtaaaaaga
    21661 agaagaagaa gaaaaatgtc gaaatcgaat atgatcctga cagcgattcg acattgatcc
    21721 ataaaaaaca taaacgcggg gaagatgagg attggggttg gtaagtgagc aacaagtctg
    21781 aaaagaaaca tgtcaggcat gttccgcaac ggacgtgcat cgcttgtcgg caaaccttgc
    21841 cgaagcgatc attgatccga ttggtcagaa cagatgaagg cttgcagctt gatccgaccg
    21901 gaaaggtctc cggtcgggga gcatacctgc atgattcgaa agcatgttgg aacattgcga
    21961 tccaacacag gctgcttgaa aagagcctga agactgaatt gagtgataaa gatttagaga
    22021 agatgagatt atggatggaa gatcttccgg ataacgctga atagcaaaca tacttgaagg
    22081 tcttctgcca taacggaaag agagtggcta tgggtgatga agcaaaaaga gtacagatac
    22141 cttatactat aaccgtccgt gatttggcga agctacttca gagtaacagc attcaggtaa
    22201 tgagaattct gatggccaac ggggtcatgg ctaatattaa tcagctggtg gacttcgata
    22261 cggcagcaat cgttgccagt gaaatgggtt tcgaggctgt acccgaatcc aaagaacagg
    22321 tagaggaatt caaagaaacc ggagatgttc cgctctggcg gcagataata aataaagaag
    22381 atccgaagag tttgattccg cgtccgccgg tcgtgacgat tttaggacat gtcgatcacg
    22441 gcaagacgtc tttattggac gccatccgtc atacaaatgt cgccgtgggg gaagccgggg
    22501 gaataaccca gcatatcggt gcctatcaga ttgaaaataa agggaaatta attacatttc
    22561 tggacactcc ggggcatgcg gcgttcagtg ccatgcgcgc acgcggcgct cagggagcgg
    22621 atattgtcgt gcttgttgtg gctgccaatg acggtgtcat gcctcagacg aaagaggcaa
    22681 tttcacatgc caaagccgca caggttccga ttattgttgc gctgaataaa atcgatcgct
    22741 ccgatgccaa cccggatttg gtaaaaaagc agctttccga acaaggcttg ataccggatg
    22801 aatggggcgg aaacacgatg gttgttccgg tctccgccaa gcagcgaatc ggtatcgatg
    22861 atttgatgga agcgattctg ttgacagccg aaaacagcga aattttggct aatcccaaag
    22921 gaaaagtatt cggaacggtt atcgaagctt ccattgatcc gaagaagggc gttatggcga
    22981 cccttttggt tcaaaacggc actttgaaaa tgggtgatgt tgttttagcg ggtcagtcga
    23041 tcggtaaaat cagggcgatg tttgattttc gcggcaagaa gattcaaaag gccggtccct
    23101 cgacaccggt tcagattatg ggtctgaccg aagtaccgac ggctggagat attttccaag
    23161 tctatgcgtc ggaaaaagaa gctcgggcgg tggttgaaaa tgcgaaaaat gaagcggcgt
    23221 taaaaagctc gacaccgaaa gccacattgg aagaactgtt tgccaagttc aaagccggtg
    23281 aagcaaaaga attgaacttg attattaaga cagatgtcaa cgggtctttg gaacccatta
    23341 tcaattccat caatgacctc agcaaggaag gcgaaattaa aatcaatatc cttcatgcag
    23401 aggccggcaa tatcagtgaa aatgatgtca tgcttgcatc cgcgtctcac ggagttgtca
    23461 tcggatttaa tgtccaagcc gacccggttg ccaagaaaaa agccgaaaac gaaggcgttt
    23521 cgattcgtat ttacgatatt atttaccgtt tgatcgaaga catcgaaaaa gctctgaaag
    23581 gaatgttgga gcccgaattt aaagaaacag ttatcgggcg agcggaggtt ttgacgacgt
    23641 tctccatttc aaaagtgggg actattgccg gatgcagagt gaaagaagga gatatccgac
    23701 ggaactgcaa ggttcatgta aagcgcggaa atgagatcat ttttacgggt gagatcagtt
    23761 cgctgaagca tgaaaaggag gatgtaaaag aagtgaaaac cggattggat tgcggtatcg
    23821 gattaaaagg atttgaagat tttaaaccgg gggatatact cgaagctttc gttttggaaa
    23881 gattcggtat gtgatgtaag agaggaggaa ttatcatgcc atcgcccata cgattgaaaa
    23941 aaatagcaga gcgaattcgg gatgatttat ccgaaatgtt gctgtatgaa attgatgatc
    24001 cgagactaca gggagtcatg ataacggatg tcaacgtgga tcgggaactt tattatgcca
    24061 atatttatgt ttccgctttg gaagggaagg aacgcagcgc cgaaattttg gcgggattgg
    24121 aaaatgcgtc aggatatatg aggcatgctc tttcgcagtc gatcgagttg aggacttttc
    24181 cgaaattacg ctttttttgg gatcctactc cggaaaaagc ggatcgggtg gaaaccttat
    24241 taagacagct caaagaagaa caggaagcaa aaaataaaaa tgaacaaaaa gggattgcct
    24301 gaaaaaggca atcccttatt tatcgaattc ttccgaatcg gaaccaaata gaacgattaa
    24361 aaaatgatta aaatggtttt agaatcggac ggggaattta tcaaaaaata tcgtaatttg
    24421 agagctcatg aatcaagaca agaacaatgt actgacggaa atccgcaaaa tcattgaaaa
    24481 cgcgacgaat attgctttat tttgccatat tcgtccggat ggcgatgcca tcggctcaat
    24541 gctcggatta ggctggtcgt tggaagagat ggggaaaaaa gtccaatatg tctgtccgga
    24601 tgaactgccg gtccaaggtt ggttgaatcc tcaaaccatc ggaaaaaaca cccgttttgt
    24661 aaaagctccg gagccgtatg attgttccgt actcttggat gtcagcacgc tttctcgagc
    24721 gggatatttt tttgaacagc ccgatatcgg gcaacccgat atctgtatcg atcatcatgt
    24781 ttccaattcc ggagatgcta agataaattt gatcgatcct tctgctccgg ctaccgcgat
    24841 tatcgtcgct catctgatcg aagaattgaa ttttccgatg aataaagaga ttgcggaagc
    24901 gcttttatcg ggcattatta tggatacgca aggattcagc acatccaata cgaatccgga
    24961 ttcgctgcgt gtcacggcga aattgatgga acagggcgcc gatttgtatt gggggatcca
    25021 aaatattttg aaagctcatt ctttggaagc ggcaaaatat tggcattgcg ggctttcaaa
    25081 attggaaacc cgaggaaaaa ttttatggac cgtgctgaca gataccgatc gggtgcactc
    25141 agggtatacc ggcagagacg atgccggttt aatcgattat ctggcttcca cggaaggttt
    25201 tcttgctacg gttttattta atgaacaaaa cgacggacga gtaaaaatat catggcggag
    25261 cagacccggt gtaaatgtgg cggcaattgc ggaagagttt ggcggcggcg gacaccggca
    25321 agcggccgga gccgatgtaa acggaccgat cgacgatgtc gttgaacggg tgctggcctg
    25381 caccgaaaaa ggtgttcgat cggctgaaca atcatcgtaa tttccttccc gagtgtccgg
    25441 ttttaattat aattgcgtga aatatattga aagaaagaaa aaatatttat gagaagtgaa
    25501 gagacgaaaa gatataaaag tgccatatcg ggtgtcctga ttgtagataa gccgattggg
    25561 aaaacgtctc atgacattgt tcaaataatc cggaaaggaa caggaatcag gcgaatcggt
    25621 cataccggca cactggatcc gagagcatcc ggtgttttag tcatattgat cgggccggcg
    25681 gttcggctga gcgaatacct gcaatcggat ataaagcgat atgaagcgac tattcggttg
    25741 ggttccgttt ccgatactta tgacggggac ggtttgatcg aaaccaccgg tgcagatatg
    25801 aatatatccg aggatcagat atcggaagcg atgaagaagt ttgtcggaga aatcgaacaa
    25861 acaccgcctg tttactccgc attaaaaatt aaaggacgaa aagcatacga aatggcgaga
    25921 gagggaatcg attttgaaat ccccagccga aaagtgacgg tctattcgat ggatttgatc
    25981 gagtataatc ccccggaact gtcggtggat atcctttgtt cctccggaac ttatgttcga
    26041 tcgatcgcaa atgatttggg acaggtttta ggatgtggag cttatctttc cggcttgcgc
    26101 agaactttaa gcggtcattt ttctttgcgg gatgccgtgt ctattccgaa tctggaagca
    26161 agttttgagg aaggaaattg gtatcaatat ctgattcccg cagctgaagc tttggacggt
    26221 tacgcggaat tggttgtaga catggatgaa gaatctcaga ttcgtcacgg cagaaggatt
    26281 ccggctcaaa ataccgattc tccgtatgcc aaagcggttt cggaacaagg tgaactgatt
    26341 gcgattctgg agttggaccc cgagacatcg gaatggaaac ccaagaaagt tctttacccc
    26401 tgagacagtg atggtacaag cggatctcat ctcacaaatt accttgaagg cttccgccat
    26461 aactatcggc ggctatgacg gtattcattg cggtcatcgt ttccttttgg atgcgatgct
    26521 caaggatgcg caccaacaat ctttaccgac ggtgatggtc acttttgatc cgttaccgtt
    26581 tgtttttttt cagaagccga agtttcccaa gaatattatt ctcccgcaag aacgggctga
    26641 gatcgccgag cagatcggga tcgattattt gatcacgttg aaatttgatc aacaattggc
    26701 tgatcagagc gcaaatgatt ttatttcgga actgtcggag tcgttggggt taaagtcctt
    26761 atgggtcgga gaagattttt ctctgggaaa aaatcgcgag ggcagtcaag agacattaaa
    26821 aaagcttgcc gatcagtttc attttgcgct tcatattttg ccgaagctgg aaatggacgg
    26881 cgggatcgtt tcatccacct ggattcgcgg attaattacc gaaggacagg tacaagaagc
    26941 cgccaagctg ttaggatatc ccttttttat cgaaaataaa attgtacacg gcgccggacg
    27001 gggaaaaaaa cttggtttcc cgacagtcaa tatgcaaata cctaaaggaa aaattatacc
    27061 tgcgatcggt gtttatacga catatattcg attgcacggg aaaaagtttc ccgcgatcac
    27121 aaatgtgggg acccgcccca cattctacaa cgatcatcaa atcgtcctcg aaacttttgt
    27181 tttttcatcg gacgggaagg atgaatacgg tgaaaccgcg cgcgtcgaat ttcttgaata
    27241 tatgcggccg gagtttaaat tcggttccgc ggatgaattg gtcaaccaaa ttcgtcagga
    27301 tatccggcat gccgaagctt ttttcgaaaa gcggaatcag ttatagagaa tttgttcaat
    27361 ttcgggtcca gatcagagaa acccaggata acaaatatgg caaacgcgat tgccaaaatc
    27421 cgttatcatg cccgccgaaa tgaatttcag cggaataagg aaatccttca aaagttaatt
    27481 gattacagaa agtctcgctt ttcattcgat aggcatcctc acttcccgaa tcaatcaaga
    27541 cccgcaacga tccgtttttt tgcttcatct gagtaatcaa cacatggact gtttggtcgt
    27601 cgaagggaga gccggggagg ctgagcaatc ctaccgaccc gaaaatatcg ggatgattaa
    27661 aagcaatatc cgctgcccaa agagctccgc gggacattcc ggcgatcgcc cggcaggacg
    27721 ggtcggtgca ggtatgatac gtttggtcaa cccatgggat gacagattcc aaaatcgcac
    27781 gatcaaaatc ggaataaaaa agatcttgta aattttcttc ttcccgaatt gtgatgacca
    27841 gaaaatacgg tagagtcaga tccttccatc cggcttgcaa atcctcggac aaatgcagat
    27901 tatcccagac ggattcatcc atattttgtc cgtgaagcaa ataaaggacc ggataccctt
    27961 tgacaaggcg tttatcgtaa caggggggaa cataaaccga aaaatcgata tttttattta
    28021 aggattcgga aaaaatttga aaatgttgtt tgtccggtgg atttgtgcaa ttcgcgacag
    28081 cggggtagaa agatcgcagg aaaagaaata gaaaagaaag aatgaataat ttatttttca
    28141 tcagcgaata attataccca tccctactgg cctatcattt tcggtttcat taatcaatat
    28201 caatcaaaat tgctgcatgg tatacttcga caaagaggtt attgtttatg acggatcatc
    28261 tacaatcaga agattcaaaa ggttttacat ttccctgtga gatgccgatt cgaattatcg
    28321 gcgaaaatac agaggctttt gaagaagagg ttatcgagat tttatcacac tatttgccgg
    28381 aggtagcccc gatcgatttc agaactcgta tgagtcgcgc caataattat tgctcaatcg
    28441 cctatacttt ttatgccgaa tcccgtgaac aggtcgaggg gatttatcgt gaactgtcct
    28501 taagcaagca cgtgaaatgg gttttatagc atgagttttc gatttgataa aaagcccgac
    28561 aggcgtgatt ttctgaaatt tgtcggattg ggagccggtg cgcttgcttt tcgtccgtat
    28621 tacagcgaaa cggagtttat tccctcggga gcgaagacgg tgcgggtggc gattgccaac
    28681 ctgagtgttt acaaagaacc gtgggatgaa agcgaaattc tctatcaaaa attcagagac
    28741 gatttgctca acgtttatta tgaagtaaat tcggaacatg gccccggcta taatcccatt
    28801 tggtatcggg tgtggggcgg ttatgtacat tccggccata cccaaattgt ggagacccga
    28861 attaatgaag tggattattc cgttaattcc acgccgcttc ccgggaagat cggcgaagtg
    28921 accgttccgt ttacccaatc caggttacac aacagcaacg gaagttggga agatgttttc
    28981 cggttatatt atcaatccgt ccattgggtg gtcggtgtac aggaggggct tgacggaagg
    29041 ccttggtacc ggattaagga tgaaatattt gatgccgatc ggttggatta ctatgtgccg
    29101 gcagaacata ttcggattgt ttcggatgcg gaaatatcac cgatttctcc ggaagttccc
    29161 tggaaagaaa aaaggattga gatttcgatc gggatgcagc aattgatcgc ttatgaatat
    29221 gaccggccgg ttttaaagac aaaagtttca accggcattc cgtccagaaa aattgaagga
    29281 caaatttcaa cctcgacacc gacaggcact tttaatattc aggataagat gccgtccaaa
    29341 catatgggtg acggaaaaat gactgccgat ctggaagcat atgaacttcc cggtgtccca
    29401 tgggtatgct tttttgaacc gcttaccggc gtagcgactc acggaacata ttggcatacc
    29461 aatttcggta tgactatgag ccacggctgt gtcaatatgc gaaatgagga agccaaatgg
    29521 atttatcgtt ggtcctatcc gcaagtgacg acccataaag ttgaaaatat cggatatggg
    29581 acaaaagtcg ttgtaagctg attcagacaa attccaaaaa gatttggttg ctgacaaagg
    29641 catatttctc attcaaaacg agattaccct cttgattgta atatacgtac ccgttagtta
    29701 agagcttttt caattgatag tgaaagatat cttccaggtt cagcccgaac cgttctttga
    29761 attttgtcgg gtttacccct tcctgtaata atcgcagccc gaaaataact acgtctttca
    29821 tttcggtcaa ttccgaaaga gcgataatct cttcggcagc cggataggtg ttttcccatg
    29881 gtctgttaaa gtttattcgt tcgatatatt cttcaatgga agatacattt ttaattcgaa
    29941 catgactgaa atagctgtgg gcgccgcttc cgaatcctaa ataaggttcg atataccagt
    30001 attgtttatt atgacggctt tcggtttgat gcgtacggct ccaattcgat atttcataat
    30061 gaatgtagcg atgctctttc atcatttcac aggccgaatc gtacatatcg gcaatggtat
    30121 cgtccggcag cgcttttaaa ctgccggaaa ggatttgact ctcgagcggg gttcccttct
    30181 cgacggataa cgcatacagt gaaatatgat ccggttccaa ctccatagcc ttttcaagag
    30241 aataacgcca ggattccatc gtctgacccg ggtaaccgaa aatcaaatcc agattgatat
    30301 tatcaaaacc tgcttcacgc agccgatgat atgtatccaa agtttgttcg acggaatgtc
    30361 ttcgtcccat gcatttcaat tcttcgttaa ttgccgattg catgccgaga ctgacacgat
    30421 ttactcccgt ggaacgcaac atcatcgttt tttccggaag aagatccccg ggattcattt
    30481 ccatggttat ttccgcatct ctcgtcacga agaaatgatc tcggacagtt tgaattaatt
    30541 cgcgaatctg ttcgggtgac aagagagacg gtgttccccc gcccaagtaa atgctgtgga
    30601 caggctgatt aatttccatg ctgatgacac agttccggat ctcttcttta acggaagcca
    30661 gatagggtgc aataaaagat tccatcccgg tgaaagtgtt aaaatcacaa taattacagc
    30721 gggttttaca gaaagggata tgaatatata ccgaaatcgg cttatccatt ctcgaatgat
    30781 accatgatat aatgtcaaaa tgtctaaaat gtactcaaag cggtctttga ccccgatcaa
    30841 gaaggtttat ggcaccggat aatttacagc aatcaagaac aaaaatttgc ccgacttgcg
    30901 gaacccggat gaacgaatcc gcgacacgct gtctggtttg cgggcatatc tttacgggaa
    30961 cggaagaaaa taaaccttct cccaaaaaat cgaaaacttc tcgaaatgac cctttgcaga
    31021 agaagccgag actgccggag gttcgtttga gtctgcccat tgcgatcggg ttgatcatat
    31081 tggcgatcgt tatcggtgcg gccgtcgttt tcctgtttct gcaaaatatg gggcgcgtgg
    31141 ttgaaccgac accgctgccg accaataccg ggacaccgac gattactttt acgccgacaa
    31201 cgacttatac gcctaccttg gttatgacag cgacgccgtt gcccccggtc gaatataccg
    31261 tcaaagaggg agatttatgc tcttcgattg cggcattatt caacatttcg attgcaagca
    31321 ttgttttgga aaataatctg acctccgact gctatctgtc tcccggaatg gttttgaaat
    31381 taccgcagcc gactccgaca gttacgccgt tgccgacgaa tacactaacc ggttccgaag
    31441 caacattagc ggcgtgcgga tcttatgact atatcgtagg gaataccgat acacttttcg
    31501 gaatcgcttt gaatttcggt gtttcgacag acattattcg tcaatataac ggtttgatga
    31561 acgacatcgt cgtcgccggt tccacattgc acattccgct ttgcgaaagg attaccccgg
    31621 ggccgacttc cactcccact aatccgcctc cctatgaagc acctaatttg ttgcttccgg
    31681 cggacgggtc ttccttcaat accagcggtg aaacgattac tctgcaatgg gcgggagtcg
    31741 gaacgttgtt ggataatgaa gcatatgaag tcaatatcga agatattacc ggaaataccg
    31801 gccgtaaact ggtcgattat gttaaggata cgaaatacat cgttccaatg tctttccggc
    31861 cgagcgatcc gtctccgcat gtgatcaaat ggtacgttca accggtaaga caaagcggca
    31921 gtcaagcgga cggtacaccg atttatacct ctgccggtgc ccgcagtgaa gctcgaacat
    31981 ttatttggtc cggcgtagcc gtccaataga ataataatag aaagacgatc aataaaaaca
    32041 gcctggaaaa ggctgttttt attgatggaa ttataaaatg tctgaaaacc aatgcaaaac
    32101 cgggtcacga tcgaccttga tggtaaaatt gtatcctatt gaggactgga aagtgagaaa
    32161 atcaacgaaa tgaaaagacc ggttgtggca ttagtcggca ggccgaacgt cggaaagagt
    32221 actttgttta accgactatc cgggaccaga gatgccattg tgaatgatgt gcccggaacg
    32281 acccgtgatc gcttattcgg acacgccgaa tgggccggtg tcgagttcga tattgtcgat
    32341 acggggggaa ttgatccttc gccctccgga caaaaagctc cactttcggt cgattcagcc
    32401 gaatttgttc aggaaataaa atctcaggca cttttggctg taagcgaagc cgatgtggtg
    32461 ctttttttgg tcgatgcaat cagcgggatt acgcccgccg atcgtgaaat tgcgtcgatt
    32521 ttgcgcaaga atcagaaaat agtagacggg aaagaaacac ctccgatttt attggttgcc
    32581 aataaagcgg attccgcacg tcaaaggcaa aacgtttcgg aattttatga actcgggatg
    32641 ggagatccat ttccgctttc cgctatccac ggtaccgaaa ccggggatat gttggacgca
    32701 ttaattgctt tattccccaa gcaggaagag gaaactccgg atgattcggt caaaatagcc
    32761 atcgtcggca aaccaaacgt aggaaaatcc agtctgctga atcgaattgt gggagaagaa
    32821 cgggcaatcg tcagcgatat accgggaacc acgcgggatg ccatcgatac ccacattctt
    32881 tataacggtg tgccggttac gctcatcgat accgccggaa ttcggcgccg gggaaagatt
    32941 gaaccgggag ttgaaaaata ttcggtcatt cgttccatgc gagcgattga ggaagccgat
    33001 gtctcattgc ttgtcattga tgcgttatcg gatattacct ctcaagatgc ccatatcgcc
    33061 ggatatattt tggaagaatg gaagagtgtt gtggtggtgg tcaataaatg ggatgccgtc
    33121 gagaaggata cttatacgat gcagacttat acggacaaaa ttcggaagga attgaatttt
    33181 atgccatatg tgcccattct ttttgtatct gctctgacca atcaacgggt caatcaggtg
    33241 attccgacgg cgctgcaagt tcaggaagaa cgcttagccc gcattacgac ttctcaattg
    33301 aatgaagtca tccatgatgc ccaagataaa catcatgcaa cctcaagaac gggacgatcg
    33361 ttgcagattt attatgcgac acaagtgcgc agtgatccgc cgacattctt aatctacgtg
    33421 aatgatccga aattagcaca tttcagttat acccgttatt tagagaatca gatccgaaaa
    33481 caattcgggt ttatcgggac accgattcga attgtttatc gcgccagacg atagccctat
    33541 ttgagccgga aaaaagaaaa taatctctaa aaacaaatag aatcttattt agattgttct
    33601 gacaaaacag gtgtattata aaagaaaaaa caaccgagcg gagagataaa acccgaatgg
    33661 caaaaaaaca accggaaaaa acaaataaaa atacgaagaa aaaagaagca gagaaaaaga
    33721 agacgacagc aggaatgatt tgggaaattg ttcagacgct gctgatggca gccgtcctgt
    33781 attttgcgat cgatttttgt atcgcgcggg ttcgggtaga gaatatcagt atggaaccca
    33841 cctttacgga aggggaattg ttaatggtca acaaactgaa ttataaattc agtgaacccg
    33901 agcggggtga tgtggtcatt tttgaggcac ccgtggcccc ggggaaggat tatatcaaac
    33961 ggatgatcgg attgcccgga gatgttgtaa cggtcaagga tggaaaattg tatatcaacg
    34021 gtgaattgat caatgaaccc tgggtacatg aaccgatcgg ttattccggt gaatgggaag
    34081 ttccggaaaa tcaatatttt gtgttgggag ataatcggaa tcattcttct gattcgcata
    34141 gttgggggtt tgtccccaga gaaaatctga tcggcaacgc atttttctgc tattggccgt
    34201 tgaatcacat tcgggtgatt aacagtcatg tgacccttca gaatgaattg gagaccgcat
    34261 cactgaaagg agaatgaaat atggaaagag tcgaatattg tgaagaatgc caggttggta
    34321 ttccgcattt taccacggcg acttatttta cctggattga ggatgaatta ataaccgttc
    34381 ctgattttcc ctgttgggta tgcgatgttt gcggtcgaag agaatgggat gccgaatcgc
    34441 tgatgaatct taatttgatc ttatcgccga atgccgggaa accgcaacca cggaaacgta
    34501 ccataacagg agaatcaaag tcaacgcgcg ccaaaacgcg ccgaagtgcc gtccgttaaa
    34561 gcggatgatt gaaataaaac gaaaacataa aaaaagcttc cggcatatcc ggaagctttt
    34621 tttatgttta ttctgccggc gccggtgccg gtgcggtcgg cgtcgtcaat gtgatatcaa
    34681 aggtccctga tttgcagata tcggtcatgt attgagcgtt ttcgacggcg atcgtatcat
    34741 tgggaatgcc ttgtgtaatc aatttgacga tttccgccgc ttcctgacaa ttcccgctcc
    34801 gggcgagaat aatgccgtac atataataca gctccggaac acggttatcg tatgagagag
    34861 gaattccttc gataatttcg ccgttgggag tggtcccgcc ctgaattgcc aacctgaaat
    34921 acgtcaaagc attggcatag tcttcggaca tataatattg cgttccgaga ttccaataaa
    34981 acatggcatt ggtcggatct aagtcgattg catcctgagc ctgctgaatc gctttggcat
    35041 attcaccgtt cccgctatac gttcgggaaa tatagtaagg tgcccaggaa tcataaggat
    35101 tcagtgatcg tgccctttca aatgcggtga tcgcttccga atcttttccg gcggctcgca
    35161 ggtttctgcc cagagcaagg taaagatccg atatattcgg attgatagaa atcgcaattt
    35221 gaaattcatt aatcgcgtcc tcatagttac cggttatttc cagtaataaa ccgcgtgccc
    35281 gatgcgtctc catcaactcg ttcccatgtt cgatcgccag tcgtgaatat tcggtcgctt
    35341 tttcgagtgt attcaaacta tcctgtcccg cattgatctg atcgacgaga atttccgttc
    35401 gatatgcaaa agtcaacggg ttattggggt cgatttcggc agctcgattg aacgcggttt
    35461 cagcatccaa ataattcccc attttgccga gcgcccaacc gcgaatcgca tgggcaacgg
    35521 cattattccc gtttagcaga atcgcatttt ccgcattttt taatgcatct tcataatggg
    35581 tctcgaacat ttggagtttc gcgagactga tataaacgga aggatttttc ggatcggaac
    35641 tgatcgcttt ctgatagagt tcaattgctt gggcatttcg cccttctttc gcgaattgtt
    35701 ccgcttcgga aataaaagct tcgggaggag tagtcggcgt attggttgga ataaacagcg
    35761 gctgtgtttc gggcgcaata taaatactga aatataatcc ggcagctacc aaaacaaaaa
    35821 ggaaaaatac tctgaatgga tttgatctcc tttttcttcc gttatagctg tatttcgatc
    35881 cttttaaata cacactttca gccctttctc aaatcatttc aatctttaga acttattata
    35941 atcgtccgag tgttcggaaa aaaagattct gattcaattt aaataattgt atttttcagg
    36001 aattaatatt atttttgtca gacaaaaaag gtggaaattt taatgggaaa cctttataat
    36061 aaaagaaggt aagtatatca acccaaaata aaaggagaaa aaaatgaaaa aaataagtat
    36121 tatcttggca ttaattgcaa tccttgtgtt ttgcttgcct gccctggcgg atgatgtgat
    36181 caaagtttgt gaagtcactg ataccggcgg tgtagatgat aaatctttta acgaaacagc
    36241 ttggaacggc attctccaag caaaagaaaa gtacggggtt gaagccgtcg ttctggaatc
    36301 acaatccgat gccgattatg ccgtcaatat cgatcaattc atcgaagacg aatgcgattt
    36361 aatcgttacc gtcggcttca tgttgatgga tgcgactgct acggcagccg ataacaatcc
    36421 ggatttcaaa ttcagtatta tcgatgcatc ggtcggaaaa cccaatgttg ttgatcaggt
    36481 tttccagacg gatgaagccg cttttcttgc cggttatgtc gctgccggga tgacgaaaac
    36541 cggaatcatc ggtactttcg gcggaatgtg cattccgacc gtaaccattt tcatggatgg
    36601 ttttgcgcgc ggcgccgatt actacaatca aattcacggt accaaggtcg aagttttggg
    36661 ctggaatacc gaaacgcttg aaggaacctg tgtcgattct ttcgacgatc ttgataaagg
    36721 aaaacaggta tccattacca tgatggatca gggcgccgat attattatgc cggttgccgg
    36781 accggtgggc ggcggaacga ttgctgctat ggaagaccgg aagatgggta tggtaatcgg
    36841 tgtcgattcc gattggtatc tgacttaccc ggataacggt gatattatcc tgacttcggt
    36901 tatgaagaag atggatgcca ctacgatgca ggtaatcgaa tccgtaatcg acggaacctt
    36961 caaaggcggt aaagttgtcg gaacgctgga aaataagggc gttgatcttg ctccgttcca
    37021 cgattttgaa gataaagtac cggctgacct ggtgaaagaa ctggatgata ttcgcgcaaa
    37081 gattatctcc ggcgaaattg agttgaaccc ggtttatgct cagtaaaatt aacagataga
    37141 gaatgttgta tcaaggtgga ggaattttcc tccacctgct ttaattaatt tcaatcattt
    37201 ttcagggatc cggtttttaa ccggtcatat tggaaaaata tctttacaaa gaaggatgtt
    37261 tttgaaaaca gcctttttga aatcatatcc caaaaaggag aaaaaatgcc cccaatcctt
    37321 gaattaaagg gaattaccaa acaatttccg ggtgttcttg ccaatgacca tattaattta
    37381 acgcttgaaa aaggcgagat attggcattg ttgggagaaa acggcgccgg aaaaactaca
    37441 ttaatgaata ttctttacgg tttatacaaa ccggatgaag gggaaatttt tatcgatggg
    37501 gaaaaaatag aaataagcgg accgatcgat gcgattcggg cgggaatcgg aatggtccac
    37561 caacatttta tgttgattcc tgtttttacg gtcacggaaa atgtgatgtt gggagaggaa
    37621 tcggtcaatt tcggaggggt tctgaatcct gaagatgcag ccaaaaaaat aaaagcgatc
    37681 ggtgaactgt acggactgaa agtcgatccg aatgccaaag tcggtgattt gccggtaggg
    37741 gttcagcaac gggttgagat tatcaaatta ctatatcgaa atgcccgtat attgattttt
    37801 gacgaaccca ccgctgttct tacaccccaa gaagctgatg agttatttga aattatgcgg
    37861 tctctgacga aacaaggcaa gtcgataatt tttatcactc ataagctgcg tgaggttctc
    37921 gaattttcgg atcgaattat ggttattcaa cgcggaaaag tggtgggcga gacacatcct
    37981 catgaagcca gccaggagaa attagccgag atgatggtcg gccgggcggt tgatctgatt
    38041 ccggataaga agaaaataca ggcaggtgaa acggttctga aagtcgataa cttggttgta
    38101 gcggacggca cgagaaaaat cacggttcac ggcgtttctt tttctgtaca cgccggagaa
    38161 atattaggtg tcgccggtgt tcagggaaac ggccagaccg agttggaaga agcattgacc
    38221 ggaatgcgtg aacctgtcag cggaacgatc cgtattttag gcaaggaagt accgaaggta
    38281 aaaccgcggg aattcgtcga aaataaaggg gcgcatattc ccgaagatcg ccaacgggac
    38341 ggattgattt tgaactttac cgtcgaagat aacctgatgc tttgtactta ttaccaaccg
    38401 ccgttctctc aaaatcatat tctcaatcgg gaagcgatcc gggatcacgc ccgtaaattg
    38461 atcaccaatt ttgatattcg tccgccggaa ccgctgctgc cggttgcttc tctttccgga
    38521 ggaaatcagc aaaaggtaat tgttgcgaga gaattaagtc gcgacaataa attgatcgtt
    38581 gcatcgcagc cgacgcgcgg attggatgtc ggttcaattg aatatatcca tgatcagttg
    38641 ctgaaaagca gagaggaagg ttgcggaatt ctgttgattt cttcggaact cgatgaaatc
    38701 atggagcttt ccgatcggat tattgtgatg tacaaaggca atatcacttt gatgacagag
    38761 acggaaaaaa caacgaaatc cgaagtcgga ttacatatgg cgggcgccgg aaacagcatg
    38821 agcgaaaaat ccgaaaatgt tgctgttcaa gccggtcgtt agaaggagga ttcagatcca
    38881 tggataaaga aaataaaggg ttgaatcgcg aacaagacgc taaaaaaatg agtacggaag
    38941 agattttcct cgaagaatta tcgcggaaaa aggcacctcg tcccggattc tgggcgaagt
    39001 tttgggatgc cattcaaatt ccgcttttgg cgattatttc cggtttgttt ttgggtgccg
    39061 taattatcgt tgtaacttct gccgacgttt atgttgcttt cggaagcggg atcggagagg
    39121 ggttcaggac aatgggcagt attgtcggca atgcctataa aactctgttc accggtgcct
    39181 tcggcagtcc gtccaaaatt atcgaagcat tgcggacagg agagaaaatc agccgtgctt
    39241 ttaaacccat tacgcaaagt ttggttgaga ccactccata tttgctcagc ggtatggcgg
    39301 tggcactcgg ctttcgatcc ggggttttca atatcggcgg cgaaggacaa atttatatcg
    39361 gtgccgtcgc agccacattt gtcggtttgt cattcgacgg gttaccgcag gctgttcata
    39421 tgccgctggc gattctcgcc ggaataatcg gcggagctat ctgggggttt attcccggtt
    39481 ggctgaaagc aaagaccggc gcgcatgaag tgattaacac cattatgatg aactatatcg
    39541 cactgcggtt ggtcgagtat ttgctcaccg gcccgatgac taagaccggc ggaggcggca
    39601 tgccgatttc caattccgtc aaaccgaccg cccgaatccc gttggtattt gctccgccga
    39661 ttacattgca ttggggcttt tttctggcta ttctgattgc gatcgcagcc tggtttttcc
    39721 tgtttaaaac cacctgggga ttcaatctcc aaatggccgg tgcaaacaag catgcagcga
    39781 agtatgccgg tttaaatatc agcctgtgga ccgtactcgg gatgaccatt tccggagcgt
    39841 tagcgggatt atgcggcagt acccaagtgt tgggtgtgac ctattcgatg ggactgggag
    39901 tttcggcagg gtatgggttt gatgcgattg cgttagcgct gcttgccaac aacaacccat
    39961 tgggatgcat cctgtcggcg ttgctcttcg gattactcaa gaccggatcc cgaagcatga
    40021 tgtccaacac cggaattcca ttggatatcg tttccatcat acaagctttt atcctgatgt
    40081 ttatcgcggc tcccgcaatc attcgcaccg tattccgggt gaaaggtctg aaagaggaaa
    40141 acaaagcatt ggttacggtt tccggctggg gaggtgacaa atgagtgagc aagaacgcat
    40201 tcaaggagga agggtaaagc tcagagatcg aatcgtcggg atcattttcg tggttctcgg
    40261 cttggtcatc tacctgtttt tttataaagc gatcgcttcc ggaatcgttt ccactttcat
    40321 tatgacaccc ggtggaatca cccgaggtgt agtggggaac tggattatac cggcaaggcc
    40381 ggtcttaatc gtattggcga tgattctgat cgggatcggc atttaccaat tctttttcgg
    40441 gttggggaaa cagggaaatc tgtttcttgc cttagcctgt gtggctttcg tgttttcgtt
    40501 tctgatatac gtttccgccg ggaaacggct aaatcttgcc agcttatttt cctcagcgtt
    40561 atcgatggcc gtgcccatca cactggcatc tttctcggga attctctgtg agcggtcggg
    40621 aatcgtcaat attgcgatcg aaggaatgat gttgatggga gctatggtcg gttccatcgt
    40681 cggatcgatc acgaaaaata tctggttggc tttagcgatg gccatgatcg gcggaggctt
    40741 gctggcattg gttcatgcct ggatgtcaat caaatataaa gtgaatcaga ttatttccgg
    40801 taccgtcatc aatatttttt cgatgggtat gaccagctat atttcgtcta tgtatcttca
    40861 gaacattcaa tcgttgaata aaacaccgcg gtttccgagt gtctccatcc cgttgcttgc
    40921 cgatattccc tttatcggtc cgattgtttt ttataacagc ctttttgttt acgggatgat
    40981 tttgttgatg attattctgc aatttgctct ctttcgaacc cggtggggac ttcgtctgcg
    41041 gtcggtgggt gaacacccga aagctgcgga tacgttgggc ataaatgtct ataaaaccca
    41101 atatacggct gttattttgg gtggtttaat ggcgggattt gccggcgctt acttttcatt
    41161 gggttcggtg ggcaggtttg acgaaggcat gacagcgggc aaaggataca tcggcttggc
    41221 tgcgatgatc ttcggcggtt ggaatccctt taatgcgatg atggccggaa tattgttcgg
    41281 atttgcggat gccatggcat catcggtctc gatcttggga tcttccatcc cggggcaatt
    41341 cattaatatg cttccgtatg tcgttacgat gattgtgtta gccggattgg tcggcgggca
    41401 cagcaccgga ccggctgcca gcggcgtccc ttatgaaaag gaaagcactt gatttaaatg
    41461 aatcattcgg cactttaaac agaaacagga gaaaactcga aaagttttct cctgttttta
    41521 tatgatgtcc atcaaagaac gatcagtctg ccgagaaaag gcagatatag ttgaagagct
    41581 aaacccgtcc gaagtcatcc tcgagccgga caatatcatc ctccggacag atccctaatt
    41641 gcgtttcgat caaaaccaaa ggttgatccg agtcgttttc aacccgatgt gcctgctctt
    41701 ttgggatttg gacgatatct ccctcgtgaa tcgggaatat tttatcccct aaggtcattt
    41761 ttccggaacc ctgaacgatg acccatattt cactcctcag atgatgaagt tgcaaagata
    41821 accgtttccc ggggaagacg ataatccttt tcacccgata attcggttcg aaaatatatt
    41881 cttcccaacg accccaaggt ctgttatcga aatcgatcat tctttctcct ctattcttcc
    41941 ttgcgctctg tatttcttac aattttatcg cacaagtccg cataaccgat accgactgcc
    42001 gcggcttcct gtggaaacag gctggtcggt gtcattcccg gcaatgtatt tgcttccaaa
    42061 caatagatat ttccgtctcc gtccacttta aaatcgatcc gggagtaata accgagcttc
    42121 aacgcccggt gaattttcaa tgccgtatcc tgtaaacgga tcgataactc ttccggaatt
    42181 tccgccggac agacttcccg cgttaatccg gattgatact ttttttcata atcaaaccaa
    42241 ccttcgtttg ggatgatttc gattgccggt agggaaatgc cctcgagaat tccgatacaa
    42301 aactcccgcc cggctaagta cttttccaca agtaaatccg gttgacggtc ctcggcggag
    42361 gcgatggcag cttgaattcc gcgtaaaaat tcctctttgt tttgcggaat gctcactccg
    42421 atcgacgaac cgccgcgggg aggttttatc acgcagggat atcccagaga atcgatttga
    42481 tcccaattca gttgatcata tcgatccaga taaacccagg gagcggtggg aaggtcgtta
    42541 gccaccatta cttccttcgc caaatgttta tccatggcaa ggacgcagcc cgcaaaaccc
    42601 gtaccggtat agtgaatatt cagcagatcg aacgtagcct gcagctgccc gttttcaccg
    42661 gccgaaccgt gaagagccaa aaaaacaacg tctgccaaac ggcagagctc caatatattt
    42721 tcaccgaaaa acatgcctgt atcggaaacc cgctgagcgg cgagtttttc aagatccgga
    42781 ggtgtcgtcg gaattttgta tgattgtttt tggtaattcg ggtatttttt aaaaagagat
    42841 tgcagccctt ggggaggtgt actgattccg agatacacgt caatcaaagc gacttcatgc
    42901 cccttttcag ccagcgcatt gctgatcatg caaccggatg aataagaaac atccctttcc
    42961 ggtgaatttc cccccgcgag cacggcaact ttcatctttt cctcccacaa aacgaaattg
    43021 gttcgttcgg aattccgact tttcgaattt tatcacgcat gaagcgaatc gatcgaacgg
    43081 gttgaaaaac ctgttcgacc gaatttaaag ccccggatga gcctatccga tatttcggaa
    43141 taagcatatc aagataccga acggaatcgg ttctattcat aaagatcaga aaaatgccgg
    43201 atcagcatcg acaaaaaaag aaaagaacaa gcgctaacgg ttttattctt gcaagagact
    43261 tatttgttcg cgccgtatga caaatacggg cggaaccgaa gaggtatctg tattttgata
    43321 ttgccggggc tcggatctaa gagcgaccgt ttcctttacc cttttcctgt tccgatattc
    43381 tttggacagc cggttccaac caatcttcct cgagcgtttc gattgcccct cgatcgcaag
    43441 cttcggatat cgcaggatcg accggcactt tcgccaaaat atcgatgccg taattttccg
    43501 caatttgttc gatatgactg tctccgaaga tcttatattc tttgccgttg tccgggcatt
    43561 taaaatagga taaattttcg atcaatccca gaatggggat attcatcatc tctgccattt
    43621 taatcgcttt ggaaaccacc atcgaaacga ggtcctgagg agaagtgacg atgataattc
    43681 catccatctt gacggattgg aaaaccgtta atgccacatc accggtgccc ggcggcatat
    43741 cgatgaacat ataatcgaca ttttcccaaa ttacatcggt ccaaaactgt tttacggttc
    43801 cggcgatgat cggcccccgc cataaaaccg gatcggtgct gtcattcaaa atcagattga
    43861 tcgacataat atcgattccg ctttttgttc gtaccggcaa aattccaaag tcgtttccgg
    43921 tcgctcggct ttgaattccg aatattttgg gaatggaggg accggtgatg tcggcatcca
    43981 aaatagcggc atgataaccg agtttttgca ttttcaccgc cagcaaggat gtcaccaggg
    44041 atttacccac tccgcctttt ccgctcataa tgccgatcac atggtcgata cggctcatgg
    44101 cgtgcggttt ttcgtgcaaa tcggccggtt tcttttgcct gctgttgcag gtctcttcgc
    44161 aactgctgca attgtggctg cactgatcac tcatgcgtta agctccttga gagttattga
    44221 atttttatcg ttcagcgttt aatgataccc gcaaattttt aaaaaagacg attttctcgg
    44281 agatcaaaaa aagatggata cctttgaggc atccatcttt tttgattatt tattaaagtc
    44341 tcgatttagt attcgggagc cggagcggga gccggttttt ccggttccgg aatgtctgta
    44401 atcaacgctt cggtcgtcag gatcatcgcg gcgatagagg ctgcgttttc aagtgccccg
    44461 cgggtcactt ttgccggatc gattacaccg ccatcaacca tatcgacata ctcgtctttc
    44521 agcacatcat agccgatcaa cttggacttc ttggcttttt gttgggcacg gatattcgcg
    44581 ataacaaccg atccgtcttt tccggcgttt tcgacgattc gatgagtagg aaccgtcaaa
    44641 gctttgcgga caatattaac accggtctga acatcttcat ccttgaattt cagtgcttcg
    44701 accttcggaa tcgcgttgag cagtgccacg ccgccacccg gaacaatgcc ttcttcgact
    44761 gccgcacggg ttgccgaaag agcgtcttca acacggtgtt tcttttcttt cagttcgact
    44821 tcggttgcgg caccgacgcg aatgatcgca acgccaccgg ataatttcgc caagcgttct
    44881 tgtaactttt cccgatcgta atcgctggta gtcttatcga tttcaacacg aatctgatca
    44941 atacgggctt taatctcgcc ggcatcgcct ttaccgccga caatggtcgt attgtcttta
    45001 tcggaaatta ccttttcgca gcggccgaga tcggaaagct gtgcggtttc taatttacgg
    45061 cctgtttctt cggaaatgac ggaagcgccg gttaaaattg cgatatcctg taacatttcc
    45121 ttgcggcgat caccgaagcc gggagctttg acagccaaaa cgttcaacat gccgcgtagt
    45181 ttgttcaata ccaaagtggc taaggcttcg ccgtcgacat cttcggcaat gatcaccaga
    45241 tcccgtttac cggtttgaac cagtttttcg agaatcggaa ccaaatcctg agccgcggaa
    45301 attttcttgt cataaatcag gatataagga tcgttgataa cggcttccat cttatccgtg
    45361 tcggtcataa agtaggaact gatataaccg cgatcgaatt tcattccttc tacgtattct
    45421 gtttcgaatt caaggccttt cgattcttcg acagtgatca caccgtcttt accgacttta
    45481 tccatcacat cggcgattaa ggatccgatt gtctcgtctt gagccgaaat agtcgctacg
    45541 tttgcaatgt catgtttggt tgtaatttta atggattgag ccttgatttc atccgataca
    45601 actttcgctg cggattcgat tcctctctta atcagcatcg gattggctcc ggctgccagt
    45661 gatttcagac cttcggaaac gattgcatgt gccaaaacgg tggaggttgt cgtaccgtca
    45721 ccggcgatat catttgtctt ggaggcggct tccttcagaa gctgtgcacc catattttca
    45781 aacgggtcgg gaagttcaat ttcttttgca acagtaacgc cgtcgtgagt tacggtcgga
    45841 gaaccgtatt ttcgatcaat cgccacattt cggcctttcg gaccgagtgt agttgcgact
    45901 gcggaagcaa cgacgtcaat accagctttt agattttttc gtgcatcttc accaaaatat
    45961 aattgttttg ccatggttta ttctctcctt cgagttctat tggtttattt agtcgattat
    46021 tgcaaggacg tcggattccc gcaggattaa caatttttta ccgtcgactt taatttcagt
    46081 tcccgcatat ttagcaaaca gaacggtatc acccgccttt acatccattt gaatacgatc
    46141 acccttttca ttgcggtcgc ccggaccggc agaaatgact ttccctttct ggggtttctc
    46201 ttttgcggtt tcgggaagga caatccctcc ggcggtcatt tcctcttcct caatgggttc
    46261 tacaactaat cggttaccta atggttttaa attcatttta ttcctccaac tatggctttt
    46321 attgttcttt ttcttaaatt tagcactctt taatactgag tgctaatgca cgacatataa
    46381 taatccgcta aaaagagatt gtcaaggtga aatttatgat tctaataaaa ctcttacatt
    46441 tcttgtgatc acattttctc tatttacaaa gttttccttc gaccataaaa tgggggtatg
    46501 aaattcgatg tgtttacgtt actccccggt gtatttgagg attacctcaa cagcagtatt
    46561 ttattaaaag cactggaaaa tcagttgatc gaagtggccg tccacgatat tcgtgaatgg
    46621 gcgccgggaa aacaccatgt gacggatgaa ccgccgtacg gcgggggtgg cggaatggtg
    46681 atgaaaccgg aaccggtttt caatgccgtg gaagcggttt taggagataa acccgattgt
    46741 ccggttattt tgttgacgcc tcaggggaag gtattcgatc agaaaacagc tcagagattg
    46801 gctcgggaac ctcatctggc attgatatgc ggccgttatg aagggtttga tgaacggatt
    46861 cgtacccact tggcgacgga agaaatatcg atcggtgatt tcgtgttaac cggaggggaa
    46921 ttgcctgcgt tggtcctgat cgatgcgatc agccgaaata taccgggggt attaggcgat
    46981 ccggacggag cgatgaccga ttctcattcg gaagggttat tagagtatcc gcactatacg
    47041 cgtcccgcgg ttttccgcga ctgggaagta ccttccgtac tgttgagcgg aaaccatgcc
    47101 gccatcgaaa actggcgtcg cgaacaatcg ctggcacgca ctttggaacg ccggccggaa
    47161 ttattggatc gggccgagtt atcggaaacc gatcgacaaa tattacagaa attaggttgg
    47221 aaaccgaaag aacgatccgc ctgaaaaatc gtttcgattg cggagttaat tcgaaccgta
    47281 tgcggtgagg aaatcttccg cgtatcggcc gaaggtaccc gaaaggatcg cgcgccgcga
    47341 ttggcgaacg agttcttcca gtgtatgaat attatgaatg gaaattaagg tgccgctgag
    47401 catttctttg gaaattaaaa ggtggcggat ataactgcgt gaaaaatgcc ggcaggtgta
    47461 acagtcgcaa ccttcgacga ttgggcgggg atcgcgggcg tattgttgat tgtttaaatt
    47521 gatgcgcccc gtaaaactca tggctgaacc gtgtcttgcc aaccgagtcg gcagaacgca
    47581 atcaaaaata tccactccgc gcagaattcc gttaatcaaa tcttcggggg aaccgacgcc
    47641 catcagatag cgcggtttat tttcgggcaa aatttcgggg acgatatcca aaacggcatt
    47701 catttcttcc ttcgtttcgc cgacggacag acccccgatc gcaaacccgg gtaaatcaaa
    47761 ggagctgaca aattccgcgg attttgttct taggtcgggg aaaataccgc cttgcacgat
    47821 tccaaacagc gcttgtttat cattggtttt cgcatcgatg cagcgctgca gccagcggtg
    47881 ggtgcgatcc atagagcgga tgttatattc ataatctttc ggatttgcgc attcatcaaa
    47941 agccattata atgtccgcgc ctaaattttc ctgaacggct atggatattt ccggggtgaa
    48001 acggtgaacg gatccgtcga tgtaactttt aaaggttacg ccgtcttcat caattttacg
    48061 cattccgtct aatgagaaaa cctgaaaacc gccggagtcg gtcaggatgg gtttctgcca
    48121 atccataaag cgatgcagcc ctcccatttc cgcgatcaaa tcatctccgg gacggagaaa
    48181 caggtgatag gtatttgcca aaatcagcgt ggcgcccgct tcttccactt gtaccggagt
    48241 caatgacttg acggttgcct gtgttccgac cggagcaaaa atcggtgtct gaaacgaacc
    48301 gtgcggtgtg tgaaataccc cggctcgggc tttgccgtct ttgccttcta tttcgaaggg
    48361 aaaattatct gaattcataa cagcggcaat tgtaccatct gtgtcaattt aaaggttttt
    48421 tccgggaagt cgtaattatt tttattccgc ttgttatcag gaagaaccgt ttgctttccg
    48481 cgccagagct ttctgcaaaa aagcatcctt ttgaacgaca aatcgcttat gaattcctct
    48541 gccgatctcc cctttttcat caaagacact gacctcaaag gtcagcatgc ggcggtcgat
    48601 ttcgatcagc gtgctctcgc aggaaatttt cattcccagc ggagaaggag caaggtgctc
    48661 gacatccaat cggaccccga ccgttgttgt accgggctca agatacggct gcacactctc
    48721 aagcgcggcg gactccatta atgcaatcat agccggggta ccatagactt gcaaatctcc
    48781 gctgccgaga tgggcggcgg tattttcgtt tgtgaccgtc tccgtttttg tgccttttat
    48841 accgatattc agcattgttt catcctttct gatttaatct ttcaacagcc aatcttccgc
    48901 ctgcttaaaa tcttcgaaca aacggaacat ttgtccgcgg tttgtttcga tcatgaaatc
    48961 cttgaatttt ccgcggactt tcgcggactc gatcaccgca gccgctcgaa tccgatagtt
    49021 gacaagtttt tgtaagacca atcccgtcaa ttgtgtggaa agatcataaa acgcatccga
    49081 aagtgcttcc ggcggcagca acaaacggtt cgtgtcgttt tcaatacacg cggataccaa
    49141 cgccaaccca tccttttctt cattgatctt gccggaaatg actttcacat aatctttccc
    49201 gtttttttgt acttttatcg tttccatttt cacatcataa tctttctttc ggcatgcatc
    49261 ggattcatcg ggtaggagcg gaagcgcttc cgatcgcacc ttttcaaccc ataacaattc
    49321 ggactcgtag aagcggcgga tgttatccgt cgttaacttt gacaatattg cctggtaagg
    49381 agtgggatcg gcgagcggag tttcatccga taacatcgca attgttcctt cgatttcgtc
    49441 cgcatatcga tccaacaaag attctgcctc ggtcttcgag agatctccgc cgaacagtaa
    49501 ttggatcgag aaaggtttgc gaatttccgg catttcagga aaatcccggg aaagtctttg
    49561 caactctttt ttaccgtttt cggtgatcga atagaacttt ttcaacggaa gcgtatcctt
    49621 ctgttcgacc tcgccaacca caaaatcctc tttttcaagc tccgcaagcg tgcgatagac
    49681 ttgattatta tttccggacc aataaagcag aggcgaacgg gccatcgctt ttttaagatc
    49741 atagccggac aagcgggtat tctgcagcaa tcccaatatg gcatatcgga tatccaattc
    49801 cgtttctcct ttcaagatat atgttaataa taacatatta tctttatgca atcaaaaaat
    49861 cggtccgaga gaatttcttc ggttaaatga tctgctgttt cggaattcga attttcctca
    49921 gtttatcaga aaaatattga ttttaaaatt gaatcgggta aaatcaaaaa ggtacacatt
    49981 tttctgtaaa ttgttggcga attgagcgtt tgtaccgact taatcgaatt atttaagctt
    50041 gcatcgggag aaaaatcgat gacgaaatat atttttgtga cgggaggtgt ggtttccggt
    50101 ttagggaaag gaattaccgc cgcatctctc ggcagattgt taaaagcgcg gggtctgaaa
    50161 gtggctgcac aaaaactgga tccatatatc aatgtggacc ccggcacaat gagcccgttt
    50221 cagcacggag aagtctttgt gacagaggac ggtgcggaga cggatttgga tttgggacat
    50281 tatgaacggt ttatcgatga ggatcttaac aaatattcca atttaacgac cggcaaggtt
    50341 tactggaatg tcctgaataa agaacggaac ggtgaatatc tcggtgagac gattcaggtg
    50401 atcccgcata tcacgaatga aatcaaatcc ttcgtctatt ccgtcggaca gaaaacaaac
    50461 gccgatgtgg taataaccga gatcggcgga accgcggggg atatcgagag ccagccgttt
    50521 ctggaagcga ttcgtcagat cggtttggaa gtcggtcagg agaactgtct gtttattcat
    50581 gtgacattgg ttccttatct caagagttcc ggagagcaga aatccaaacc gacgcagcat
    50641 tccgtccgtg aattacaatc gatgggaatt ttcccgaaaa ttattgtttt gcgctgcgat
    50701 gaaccgatcg agcccgggat cgttcagaag atttcgttgt tctgtaacgt taaaccggat
    50761 tgtgtgatcg aaaatcaaac gattccggtg ctttatgaag caccgattat gcttgaaaag
    50821 agccattttt ccgaaattgt ctgccgggaa ttgaatataa acgctccgga catcaatctg
    50881 accgagtgga ataaaatgct ggatcggatc cgtctctgtg acaaggtcgt caagatcggt
    50941 ctggttggga aatatgtcaa actgcatgac gcctaccttt ccgtgatgga agcgttacag
    51001 catgccggat atgaattggg aacaaaaatt aaaatcaaat ggatcgattc ggagacgtta
    51061 aaacccggaa attacgaaaa aattctttca gattgcgccg gcatcattat tccgggcggt
    51121 ttcggcaatc gcggaatcga agggaagatc ttggcggctc gctatgcccg agaaaataat
    51181 gtcccgtatt tgggattatg tctgggcatg caggtagctg taatcgagtt tgcgcgcaac
    51241 gtgtgcggat tgcaaggtgc taattcccgt gaattcgacg agaaggccga acatttggtc
    51301 atcgatttta tgcccgatca aaacgataca atcgcaaaag gggggacgat gcgcttaggc
    51361 agttatccct gtaaaattgt ccccggttcg atcatggaaa agtgctatca gacggatatg
    51421 atcgccgaaa gacatcgcca tcgttatgaa ttcaacaatc aatatcggga aattcttaca
    51481 aacaacggaa tgatcatcag cgggacatct cccgatggaa cgattgtcga gacggtggaa
    51541 ataccgaaaa atgatttctt tgtcggagtt cagtttcatc ccgagtttaa aagcaggccc
    51601 aaccgagcac atcctttgtt caaaggattt gtcgcggcgg ctttgaaaaa aaccgaaaaa
    51661 atgaatgagt cgcttgaatg atttttcaaa attcgttgga tttccaacga atttttattt
    51721 tgttctccgg taaactcaag gaaacggttg aatcatattg aatgagcttc ggccggataa
    51781 agaaaagtcg agcgtgttct caaaggagaa atatcaaatg gaaaatacaa tgttttgttt
    51841 tcaatgtcag gaaacagccg gaaacaaggg ctgtacacgt accggtgtgt gcgggaaaag
    51901 cgcggatacg gcgaatttgc aggacttgtt gcgttatgta acgatcgggc tgtcggttgt
    51961 gactacccga ttgcggagcg aaggaaagaa ggtcgacaaa gaggccaacc atcaggtcac
    52021 gacgaatttg ttcacgacca tcaccaacgc caattttgat gatgaggcga tttacgaacg
    52081 gattgaaaaa accttgaaaa tgaaggaaaa tctgtcggcg aagctctccg atcggcaaaa
    52141 tttaccggat gccgccacat ggcagagcca tgaccgttcc gaaatggcgg aaaaggcaaa
    52201 gaccgccggc gttttgagaa cagcggatga ggatgttcgc agcttacggg aactgattct
    52261 ttacggattg aaaggtttat ccgcttatgt gaaacatgcc aatgtgttga aacatgaaga
    52321 tgaatcgatc gatgcgttta ttcaaagcgc tctcgccaaa acgttggatg acagtctttc
    52381 tgtgaatgat ttggtcgctt taacacttga gaccggcagt tacggtgtca agggaatggc
    52441 tttgctggat gcggctaaca ccgagaccta cgggaatccg gagattacaa aagtgaattt
    52501 gggtgtccgt aaaaatccgg ggattttgat ctcgggtcat gatttgcggg acttggaaat
    52561 gctgctggaa cagacaaagg gaacgggggt ggatgtatat actcattcgg aaatgctccc
    52621 cgctcaatat tatccgtttt tcaagaaata tgatcatttt gccggaaatt acgggaatgc
    52681 ttggtggaaa cagggtaccg aattcgaaag cttcaacggg ccgattttga tgaccaccaa
    52741 ctgtattatt ccggtgaagg acagctacaa ggatcggtta ttcactacgg gtgccaccgg
    52801 atatccgggc tgcaagcata ttcccggaga aatcggggaa gaaaaagatt tttcggaaat
    52861 cgttgctttg gcgaagacct gtccggctcc ggtcgaaatc gaaagcggcg aaattatcgg
    52921 cggatttgct cataatcagg tggaagctct tgcggataag attgtggacg ccgtcaaatc
    52981 cggcgcgatt aaaaagttta tcgtcatgag cggatgcgac ggacggatga aatctcgttc
    53041 ctactacacg gaatttgcgg aagctttgcc aaaagatacc gtgattctta cttccggttg
    53101 cgcgaaatat cgctatctca agttaccctt gggtgacatc aacggaatcc cccgtgtttt
    53161 ggatgcaggg cagtgcaatg attcttattc atgggcggtt attgcattga aattgaagga
    53221 aatttttgga gcggcggata tcaatgacct tccgatcgca ttcaatattg cttggtatga
    53281 gcaaaaggcg gtgattgttc tgctggcatt gctgtctctc gggataaaga atatccatct
    53341 cggtccgaca cttcccgcat ttttatcacc gaatgttgcg aaagtattgg ttgaaaactt
    53401 cggaatcgca ccgatttcga gtgtcgaaga agatgtaaag attctttgcg catagttgta
    53461 atacaatcaa aacagccggc aaaccacgcc ggctgttttg attatctcta agcggaacct
    53521 taagaagact tcatcacaga atcgattttc acctgaattc gattgaactc ctcgcatgaa
    53581 aagagcatga ttccggtgaa agcaaaaagg acaaagggaa tcatgatcat ggttgtccgg
    53641 gtgccgatcg caccgagagc ggggggaacc aaagtggaac ccaagtaggc gacagccatc
    53701 tgaattccca taatttcctg cgcatgttcc ttcccgaaac ggaccggtgt ctcatgaagc
    53761 atgcaggggt aaatcggagc gcagcccaac cccgcaataa aaatgcctgc ttgaacgaag
    53821 ggtgaaggca gcggaaaaat cagtaaagtt aagccgataa gaataatcaa ttcgcccaaa
    53881 cggatcatca ttttattgct cagtttgaac gtcgcaaaac cggagagaaa ccgtccgatg
    53941 gtaattccgg caagaaacaa agaaatccaa gcggcggcgg tggcgggtgt cacattgcgg
    54001 gaggcgatga ggtaagtggc tccccaaagc atcatcgttt gttcgactcc gcaatacaaa
    54061 aagattgaaa tcaataccgg aaagaccccc ggatgtttga gcagaacgga aactccgctg
    54121 ctttttttcg tttgcgataa atccgattcg atcggcggat tcaattcttg ttgaattttt
    54181 tcccgctcct ttactttttt ccaaagcggc agtgacagga aaaggattgc ggcgagacca
    54241 aactggatga tcgagataat cagataaccg tttcgccaag tctgtccgga tgcgaacaat
    54301 tttgagataa tgatggggcc ggtcatggcg cccaatcccc aaaaactgtg cagccaactc
    54361 atatggcgtg attcgtaatg ttccgccaca aattcattta agccggaatc gaccgcaccg
    54421 gcgcccaacc ccagcggaat cgcaaagatc aaaaaccaat aataagaagg ggcaaagtga
    54481 aaaaggaaga gtgcggttgc ggtcatcaga acgcttacaa aagtgactac gccggtgccg
    54541 aaacgcttga taaaacgact gctgaataaa ctggaaataa tcgttccgcc ggctaccacc
    54601 ataaaaatat aaccggcaaa atcatcgggc gcatgcagtt cgattttcat catcggccag
    54661 gctgatccga gtaccgaatc aggcaagccg agactgatga aggaagcata aatcaagatc
    54721 aggaaaaaag ttgccattat gaaatatttc cgttcgttct ttccggaaaa gacaggaaaa
    54781 acgatccgtt ctgtccggaa attattttga ttgatttttt aatatattta atttacatat
    54841 aaattttgta acaattaaaa atcgccgtaa atagagttgg ggaaagatta tttcttttcc
    54901 gcggcaattt taacaatttc aatgatgatt cgtgtacaga tttccagacc ttcttttgtg
    54961 atatgttcat acggtccatg gaatccatat ccgcctgtac aaagattcgg gcaaggcagt
    55021 ccgaggaggc ttaatcgggc accgtcggtt ccgccgcgaa tcggggcggt gaccggtttc
    55081 aagccgacat ttttaatggc ttgcttggcg atatttaacg gttcgggatt cttttggaaa
    55141 accgccgcca tgttttcata ttgatctttc agggtcagtt taacggtgcc tttcccgtat
    55201 ttttcgttga ttaacttttc gatcagtttt aatgattctt tccgggcgtg aaaaatttct
    55261 tttgagtgat ctctgacgat atattttaag gttgctttct cgacatcgcc ggcgatatcg
    55321 cacagatgat aaaagccttc gtagttctct gttttggaag gaatttctcc ggaggggagc
    55381 atttggttga tttcacaggc gatcaaagcc gcatttacca taatgttttt ggcggaaccc
    55441 gggtggacat tgaccccgtg aatttcaaaa gtcgcagcac atgcgttaaa gttttcaaaa
    55501 ttgatcgttc cttcctcatc tccgtccgaa gtatatgcgt aatcggcacc gaacgcccgt
    55561 atgtcgaaat tttccgttcc ccgtccgatt tcttcatccg gtgtgaaacc gatgcggatt
    55621 ttcccatgag gaatcttttt ttcgatgatt tcttcgcagg cagtcataat ctcggcgatg
    55681 ccggccttat cgtccgcacc caataacgtg gttccgtccg ttgtgattaa cgtacatccc
    55741 tttaattttt tcaaacgagg aaaattttta acggataaga ccttgccgct tttccccaaa
    55801 ggaacatttt tcccgtcgta atcggggata atctgcggat tcaccccttc cccggaaaaa
    55861 tccggactgg tatccatatg cgctatgaaa ccgatcaccg gcactttttc atagccgggg
    55921 gttgccggaa gacttccgaa taaaacacat tgatcggaaa gggtgacttc ggttaaaccg
    55981 agagcgttca tttctttttc aagcgattcg gcaagatgga attgtatttt tgtgctgggt
    56041 gtgccggtgg aatcgggatc ggatgcggta tttattttta catattctaa aaatcggtct
    56101 gttgttttca ttaatgcctc aatgtttttc ggggacgaat gccgctgaaa gaatcacttc
    56161 cgcgtgacga acggaaaaaa aagatcgaat ccacgataat cggtatcttt ttcggtaatt
    56221 tttgcttccg gaacgggaac attcgatcga aatgtgatca cttttctaat aggaacggca
    56281 tttgttttgc ttttcattac gcttgatttt agcatatttt gagttcggaa tctgcgccgg
    56341 agatttcatt tttgcggtat tatataaaaa tcccgcccgg agtctgtttg aagcaaagaa
    56401 atcataaaaa tggcgggaat aagaatcgaa atcatgcgga aagaggaaaa aatgtttatt
    56461 cacattgtta cttggaaatt gaaagaaaat gccgaaggcc gttcgaaaga agaaaatatc
    56521 cgattaatct tgggaaaaat caatacctta ccggcactca tccctcaggt aaaatctttg
    56581 aaagccggag cggatgtgtt gcatacaccg gcttcttatg attttgggct ggttggcgaa
    56641 tttgaaaacc aggcggattt cttgatttat cgggatcatc cggaacatat aaaagtcgct
    56701 caattgattt caaatctgac aactgacaga gtttcgctcg attttgttgt ttaatctttt
    56761 atccttcttt ttccgattgc gctttgcaat gatcggatag gattttataa gcaagcggga
    56821 atcctttttc caaatgaacc ggtttcgaca ggtcttttga aagcataccg tgttgcgtat
    56881 aaccggtggc gcagatgata ccggtttcgg cgtttttaac gatatactcg atggtcattt
    56941 tatatccgtt gaaatgaaca atttcagccc ggatttcagc cgtttcttca tagtgaagcg
    57001 gatgtttgta ttcgcaatgg gacaccagta ccacagaaat aattccggct ttttccattg
    57061 catcataggg taacccgatc tgatccatgt aatccgtccg gcattcttca aaccagcgca
    57121 gataattcga attgtgaatg atccccatct gatcggtttc ataatattgg gctttatgat
    57181 aataaggttt tatttcttgt tcggtcaatt ttctcaactc ccttcttata ggattgtgtt
    57241 ttcttcaata ttcggaagat atctataaat atttgaaccc gttttccgat tgaaatagtc
    57301 ctttaattca aagattttat tccaccgctc aacactgtct ttattgatac catatctcct
    57361 ggcaacatcg atcattcttt tttcgattat acagaaaccc gaggggagag ccagacaatc
    57421 accgagctga accagcaaat catagtcatc aaattcgata ccttgaatgt aacgattgaa
    57481 aaattcaaag tcttcttcgg aaatatccca ataatccaac gatttggcga gatcttttaa
    57541 aacttgcgaa tgggttaaac agactcgagc gttggcatcc caacctctct ccatgcatat
    57601 gcgataaccc tcaatttggt gtctgccgcg aacaacaccc acataccgtc cgatatcatg
    57661 cagtaaaccc gtggtatatg ccttttcggg gtccatatcc ggacaatgtt ccgcgatcaa
    57721 acgacatgcc aggccggtat attttgaatg accgatccat cttcccggat tggagctttg
    57781 cgcccacgcc aggatttctt cagcttcttt cgattccgcg taatgttttc ccgaatgatc
    57841 cgttttcata ttcgaatagg gtctgtgaac tcatcgatga ctgagggaag attcaacacc
    57901 gcctgccagt cttcgtaaac ttctggaaat tgcctttgca acaaaatggg tttgccttct
    57961 tttgagatga cgccgtattt tatccgtccg gtcgtgcata cggagcgaga atttgcattg
    58021 attattttaa agttgatagt catcttatat ccggtaaaat ggaccatttc gctgactaca
    58081 taaaaatcct cggcaaattc tgccggagat aaaaattctg cggaagactc ggcgaccgga
    58141 aggaaaattc ccatttcttc gatcactttc aacggatatt tggtttgatc catataatct
    58201 attctggctt cctcaaacca ccgataataa ttcgaattgt gcatgatacc catgcggtct
    58261 gtttcatgat attgtacgcg acgtttataa gcttttatcc tgtaagtcat gattgatttc
    58321 cctgatttgt ccgattcggc aaggaataaa aaattcaatg cgatcggatt attttaacca
    58381 caggtttaaa ttataacgct caaaaaaggg ataaagaatt tgtctctctc aaagcagccg
    58441 aaggacggga ggtttgatgt cattttcggt aagtccgagc cggggaagat cagcaggctt
    58501 accgcttggg tattttccga aaatttttat aaaggaagat tgatttgtat tcaatatttt
    58561 ccgtattgcc ggagcgtctc ccacattgcc atgaagtttt caaccggtac atcactttgg
    58621 acgttatgaa cggctgcgaa tacaaagccc ccgtctttgg caaggtcgtc aatccggtgt
    58681 ttgacttctt cacggacttg atcgggtgtt ccgaaaggca agattttttg agtatcaacg
    58741 cctccgcccc agaaagtgag atctttgccg aattctcgtt tcagttcagc ggtgcccata
    58801 tcttttgcac tcacctgaac aggattgaga atattcacgc cttcttcgat taggaacggg
    58861 ataaacgctt tgcaggatcc gcaggtatga tagaaaatga aaatctcatt ttttgctttt
    58921 gaatggatga attggtagag ttccttgtga taaggatgaa tcatctcttt atatagacga
    58981 ggggaaatca gcggtccgtt ttgagtggcg aggtcatccg cttcggagac aatcaggaca
    59041 ttctcaccca atttttccaa cgccttttcc cagaaagcca atttataatc cgtgattttc
    59101 cgcatgaccg catgtgcata ttctttttcc gaaatcatat ccatgaaaaa cttttcaaat
    59161 ccatttgtcc ataaagccat ttcccaaatt ccggcgcaat cccgcgcaat aacggatgct
    59221 tttttgtctt gaaggatgat cttatcattt cgctctcgaa tcgtcgcaaa gcgcccttca
    59281 tctgccggat ccggccaacg gaacgcttcc aaatcggcga tggtatgagc ctttccgaga
    59341 ggatgcttga ccatatcaaa ataatgcccg ttttcaaccg gcatgctcca ttgaattccc
    59401 cattcgtccg ttcgggtata ataacgccct tcccgtgtga ccggtttttc aagtcccgga
    59461 ttaagaggcg gatcagtcgg aacattacag acatctactt ccaagatttc ttgaacatct
    59521 tcatcaatcc aggcgagttg ttgttttaca tccgatattt tcggttcaat tttgggcaag
    59581 ccgagagaat cacggagctt tttgtaaaga tgaatgttca tgccggtaac ttcggtaccg
    59641 ccgagatcaa aaggaatatg atcgggctct ttgtgatgga gagcggcgga aacacgttca
    59701 taaccggaaa atttttgagg ttgaggattc atcggattat tcctttgttc tgatagatat
    59761 tatacaaaga aagtgaaaaa tcttttgcat attttgtaaa tgatttcatt tcaacgaaaa
    59821 ggaaaaatgt catcatttca tactgaataa aatcctaaaa ttttagtaaa acagcgagac
    59881 tttttatatg aactattgac attcgttttt accatggtag actgttaaga aatgcagaaa
    59941 ttaaaaacca tatttgaaaa ataaccgaat aacagagaaa ataagcaaaa tttttgaaag
    60001 atcatgccat ttgaatttgc aaaagggaat ttgaagtcaa tgaaattcaa ctaaaactta
    60061 tctaaaataa gctaaacaag tactaaaata aatggcgatg gtaagaatga aaaatgcgat
    60121 tcgataggga aaaacttttg aacaggatga actgtttgaa agaattaata taaaaagaaa
    60181 ggttgtagga gatgaaaaag ttatcagttt ttacatttgt tgttctattg gtcgttctca
    60241 tgatatcttc cgtaacggta tttgcgaaag atgattatac gattgcggtc gttccgaaga
    60301 tgaccagtat tgcctggttc cagaggatgg aagtcggtgt caatgattat gtaaaagaaa
    60361 ccggtgttga tgcattctat accggcccgt ccgaaggaga cggcgctctt caggctcagg
    60421 ttattgaaga cctgatcgct cagggtgtcg atgcgatttg tgtggttcct ttctcaacgg
    60481 aagcattgga acccgttttg aaaaaagcac gtgaggcggg aattgtggta atttctcatg
    60541 aagcagccgg tatggaaaat atcgattacg acattgaagc ttttgataac agcgcttacg
    60601 gccgccattt tatggaacgg atcggtgagt taaccggcgg agaaggtgaa tatgttttgg
    60661 aagtcggttc gttaacttcc gcttctcaca atcaatgggt agacagcgcc aaagctttac
    60721 aggaagaaaa attcccgaaa atgaagcaat acggtgacaa gattgaaacc aaggatgatc
    60781 aatccatttc atatgaaaaa attaaggaag ttttaacagc taatccgaat attgcagcca
    60841 ttcaagggtc tgccatggcc gatgttgccg gtgcggcgct tgcggtagaa gaactcggct
    60901 tagccggaaa ggttaagatt gtcggtacct cattggtttc cgtttccgga aaatatgtac
    60961 gggaaggcac aatcgacatg atctccttct gggatcctgc attggcaggg aaagctatga
    61021 ttgccttagc tcaaaaagtc ctggacggtg aaaagatcga atccggtttg gatttgggga
    61081 tcgaaggtta ttccaacctt acgctggacg gaaaagttct taccggacag gcttgggtcg
    61141 atgtaaccaa agacaatgtt gacgatccgg catataattt ctaattaatt cggggaatct
    61201 ttttagcgga ttgaattaaa taccccataa agagcccttt atggggtatt tgttaagaac
    61261 gaatattgat ttcatgacag acaggaggct gcacatggca gagacgtttc tggagctgaa
    61321 ggacattcac aaatcatttg gcggtgttca agctttgaag ggcgttgatc tgacaatcag
    61381 atgcggtgag attcattgcc ttgccggtga gaacggttca ggaaagtcca ccctgataaa
    61441 agtaatttcc ggtgcgcatg aaccgacaaa aggtgagatt tatatcgaag gagaaagaat
    61501 ccttcatctc aatccgatta tcgccattga acacggcatt caggtgattt atcaggattt
    61561 tgccattttc ccgaatctga ccgtcgcgga aaatatcgct attaaccgag cggttagtac
    61621 cggcgcaaaa ttgatgaatt ggaagggcgc cagggaactt gcgcttgaag ccatggagca
    61681 gatcggtgca aaaatggatc cggatgtttt ggtggaaaat ctgtccgtcg ccaaccggca
    61741 aatggtggcc atctgtcgaa caattatcaa tgatgcaaaa ttgcttatat tggatgagcc
    61801 gactgcggca ttgacctcta aagaagttca aaaactgtat gcgattctcc gcagactgcg
    61861 cgataagggt attgcggtaa tgatcgtcaa ccacaaattg gatgaagtat ttgaaatcgc
    61921 cgaacggctg accatattgc gaaacggtga aaaaatttcc accgggatga ttgatgaata
    61981 tgatcggaat cgctttgtca aggaattgac ggggagaaat atttccgaat ctttttatga
    62041 accgaaagtt tctgaagaag aaatattccg ggtggaaaac atcagccgga aaggcgcttt
    62101 cgaggacgtg agcttttcac tgaagcgcgg agatgttttc ggtattaccg gcttgttgga
    62161 ttccggtcgc gggcagatcg gtgatgcatt atttgggatt aatccgattc aaagcggaaa
    62221 aattttctta caaggaaaac agatcgaaat taattcaact gaagatgccg tccgtcacaa
    62281 gattgcttat gtcccggaag accgcttgac tcagggattg tttttggatc gatcgattca
    62341 ggataatacg attgcggctt ccatttcgaa atattttatt cgcggaaagt tagatgaaca
    62401 ggcaatgtat cgagccaccg ttgaatggat caaacgaatc ggatgcgtgg ctccctctcc
    62461 ggaagtttcc gttcgcacgt tatccggcgg aaatgcgcaa aaaattgtga tcgcgaaatg
    62521 gttgaacacc gatccggcgt tgttgatatt aaacggtccg acggttggtg tggatatcgg
    62581 cgccaaatat gatattcatc agattctccg tcgcttggcg ggggaaggga tcggagtgat
    62641 agtcatatcc gatgatcttc cggaattaat acagaattgc aaccatatta tggttatgaa
    62701 aaacggacgg gtttcggaaa ttgtcgagcc gtcggaagtg aatgaaaccg agctcgccac
    62761 aatgatgagc agccaatgag cgagggaatc gatgaatcag aaaattagaa atttccttac
    62821 gtcaaatgaa atgatcgtcg tttatattat tgccggattg gcgatcctga tcggatcggt
    62881 gaaccatgcc ttcaatagta tttctaccgt ggtaaatttg gccaggacaa tgctggtgac
    62941 gttaattttt gccatgtgtg aaatgttggt cattgtttcg ggcgggattg atgtttcttt
    63001 cccggcgatt gcttctctgg cgatgattgc caccggtaat attatgttga agaacggact
    63061 tgacaacgtg ccgcttgcct ttttaatggg agcggttatc gggggcgcgc tcggcacatt
    63121 gaacgccgta ctgattgcgg ttgtgaagat gccgccgttg attgcgactc tcggagtgag
    63181 cagtatgacc agtggagcaa cgttggcaat attcggcgca aaaattttga ccgttctccc
    63241 ggcgaaactc gatgagcttt ataaagtcag cctgtttacc tataccaacc ccgaaggaat
    63301 tacctactca ttaaccgttt tgatgttgat tcccatcgga atttgtctgt tgatgtggtt
    63361 tcttttgaaa tatacgatgt ttgggcgggg aatttatgcg acgggcggag atattcgggc
    63421 ggcaaggata gccggattta atgtgaccgc aatccaattt ttcgtttata tcgtgtccgg
    63481 cattttgtcc ggaattgccg gcatcaccta tatgattctg atgcgggcgg cggatccttc
    63541 tgtcttgatg ggcagtgaaa tgatggtgat tgctgcggtg gtgatggggg ggacccgaat
    63601 taccggcgga catgggacgg tactcggcac gattttaggt gtcactctta tttcattgat
    63661 taaaaataat ttgatcatgt tgggcgttcc cacccatttt cagacatttg taatcggatt
    63721 gatcatgatt gtcggtactt ccataacatc gctgaaagcg aagcatatcg caaatagcgc
    63781 aaagatttga ggcggcaggc tgaaatgcaa aaaataacga attatttcaa aaaagcggat
    63841 cggattgctc ttgctctgtt aatcggctct gtcttgattt tcgttctgat gggtgtgacg
    63901 catccgaagt ttttcggaaa atccaacctg caaagtatgt tgattcaatt tccggaatat
    63961 ggaattttgg catttggaat gatgattgcc atgattgccg gtggaattga tctttcctta
    64021 gtcggaatcg cgaatttatc cggtatcgtc gccgctgttc ttatgctgag aatgggcggg
    64081 acgaatcttt ccatcggaat cggcatcgtc gcagcggttc tggtcggtgg tctgtgcggt
    64141 ctttttaacg gattcattat cggttttttg gacattccgg caatgctggt aacactatgc
    64201 gggctgcagc tgtattcggg tttagcattg atcattacca aaggtcccgc aattaccggt
    64261 ttaccggaaa ccttcagtaa catcgcgaac ggtaaactgt tgggcgttcc gatcggggca
    64321 atcgtatttg ttctgatcgc cattctcctg tgttatttac tttattacac ggtctacgga
    64381 aaaagcttat acctgatggg gacgaataag atcgcttccc gattttccgg aataaacaac
    64441 cttcgaatca ctatgcttac ttactgtcta tccggaatct tcggcagttt cgccggaata
    64501 ctgatggcct ctcactataa ttccgcgaaa tcggattatg gcacctctta tacattgctt
    64561 accttgttga tcgtggtttt aggcggtgtg aaccccaacg gcggtcgcgg aaaggtgatg
    64621 ggagtggtga tgtcaattat tctgttgcag ttggtatcca gcgcttttaa tattttaaga
    64681 gtgaatgcgt ttgtgaagac ttttgcatgg gggttgatat tgattttagt tactaccgcg
    64741 acctatttga cggctcagaa ggaagcgcga gattaactgg aatcgcgaaa gattaatttt
    64801 tgcatcgaaa aaaataaaaa agaatggggg aaataatgag aacaattcat gcacaaaaat
    64861 tatccaaaga agctttttct ccatacgggc aatattacaa tttgatgagc cccgaaggat
    64921 ataatttgga cggttttttc ccggacagag ttttaatgcc gtttcccggt ggtattccgg
    64981 ccggtttttc accgttgata accgaaaaac ctgcggaata tattattgaa aagtcggaat
    65041 atcataacta tgctcaggaa gggatccttc cgatcgacag tgatattctc gtttatgtcg
    65101 ctccggcttc aaacaaaccc gtaccggaat taaccgaagc ttttctggtt ccgaagggga
    65161 cctttgtcgc gttgcgaacc ggtgtatggc atatgggacc tttcccggtg gatagagaga
    65221 tagcgcacat tttgattgca cttcccgaac gaacttatat gaatgattgc catgttgttg
    65281 aatactcaca ggatgatcgg attaaaatcg aactttgata ttatttgata atatttaaaa
    65341 ataaggatta aaaatgaaaa aaattattaa tacccctgaa aaatttatta atgaaatgct
    65401 ggaagggata tatgccgctc atcccgatat ggtgaaattt gtcggcgatg atcttcattg
    65461 tctggtcact accaaaaaag ttcccggaaa agtcgggatt gccaccggtg gcggttccgg
    65521 tcatttgccg ttatttttgg gctatgtcgg cgaaggaatg ttggacggat gttcgatcgg
    65581 tgatgtcttt caatctccca gtgccgaaca gatgttggcg gtgaccaaag agattgattc
    65641 cggagcggga gttttatata tttacgggaa ctacggcgga gatattctca actttgatat
    65701 ggctgcggag atggcggatt ttgaagaaaa tattcatgtc gaatccgtcg tagccggaga
    65761 cgatgtggca tccggcgcac ccgtgaaacc gggcgcaaaa aatactcgac gaggcgtcgc
    65821 cggaattttc tttgtttata aatgtgccgg ggccgcggcg gcaaaaatgt tgccgctgga
    65881 agaggtgaag cgtgttgcgg aaaaggtttg tgaaaatgta cgaacgatgg gggtagcgct
    65941 gactccgtgc accgttccga gggtcggtca tccgagcttt gagttggcgg aagatgaaat
    66001 ggaaatcggc atggggattc atggtgaacc ggggatccgg cggggaaaat tactgccggc
    66061 cgatgcgatt gtcgatgaaa tgttgaatca gattattgac gatcttccgt atcgagccgg
    66121 agatgaagta gctgtattgg ttaatggatt aggcgcgact cctttggaag agcaatatgt
    66181 catgttccga agaatcgata aagtactttc ggaaaaagga attaaaatat ttcattccta
    66241 tgtgggtgag tatgccactt cccttgaaat ggccggagct tccatctctt tattcaaatt
    66301 ggatgacgaa ttaaaggagt tgttaagggc gcctgcggac acaccgtttt tcaaacaatt
    66361 cagctattaa tttcggaggt tgtgagcatg ctggatgcaa atgatttcaa aaagctgatg
    66421 caacattggg cggaggtaat ggtcgcaaat cgagattacc tgattgaatt ggacagtcat
    66481 gtcggcgaca gtgatttagg attgacgatg ggagacggtt ttaccgccgc gagccgggcg
    66541 attgccgatt ccgatgagac cgatatcggc aagctggctt ataccgcggg aaaagccatg
    66601 tccaccgcag tcccgtcgac catggggact ttaatggctt cgggcttgat gtcggtcgga
    66661 aaagcactcc gcggaaaaac ggaacttgac agccagggag tggccgatct tttccaagct
    66721 ttttttgacg gtgttcagaa tcgcggaaag gcacaagtcg gtgataaaac atttttagac
    66781 ggcctttacg gcgcggtcga atccttaaaa gcggatgcgg cggccggagt atccatgatc
    66841 gaagcggcaa aaaaggctgc agaagccgcc catcggggat ttctcggtac gaaaggcatg
//
LOCUS       DAOSMM010000004        62386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_514_length_62386_cov_17.247902, whole genome shotgun sequence.
ACCESSION   DAOSMM010000004 DAOSMM010000000
VERSION     DAOSMM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 62386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62386
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_514_length_62386_cov_17.247902"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(942..1014)
                     /locus_tag="PK898_01465"
     tRNA            complement(942..1014)
                     /locus_tag="PK898_01465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(979..981),aa:Arg,seq:tcg)
     gene            complement(1075..2346)
                     /gene="hisS"
                     /locus_tag="PK898_01470"
     CDS             complement(1075..2346)
                     /gene="hisS"
                     /locus_tag="PK898_01470"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU18732.1"
                     /translation="MMKEIVQKVKGTRDFYPEDMAFRTWLYHQIRIVSERFGYQEYEG
                     PMLEKLELYAAKSGEELVKEQAYVFNDRGGGEIALRPELTPTLARMVAQKQKELVYPL
                     RWWSFGPFWRYEQPQKGRTREFFQWNIDLIGAAGYEADAELLAICAAFFEQLGLTSEQ
                     VQINVNNRRLMDAQLEKIGIDQDLKPAVYKLIDRCDKLRPDAWREYASECGVSPVQLN
                     ALTVLLENRDLWKESEELSTIFKLIQAMGYEDYINYDPHVIRGLLYYTGTVFEARDKA
                     PEGRALSGGGRYENLVADVGGDPLSGVGFAMGDVMISVVLEKYGKYPELSTQPARIVV
                     TCFNSETLPETYGLAEEIRRSGINVITYPEAAKLDKQLKFANRIGARFALIAGPDEIS
                     AGKVTVKDLTNRTQETIEKQDLIEFLKQSGF"
     gene            2461..2820
                     /gene="hypA"
                     /locus_tag="PK898_01475"
     CDS             2461..2820
                     /gene="hypA"
                     /locus_tag="PK898_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOU18733.1"
                     /translation="MHELSATQNILRIVMDHAERNHADSVSAINLVVGDFANIVPDCV
                     GYYWKIITEGTIYAGAVLHFDRRPAIFECDDCGSQFEITREMTACPQCGSDHVTFISG
                     DELLVESIEIVRNEKEK"
     gene            2817..3488
                     /gene="hypB"
                     /locus_tag="PK898_01480"
     CDS             2817..3488
                     /gene="hypB"
                     /locus_tag="PK898_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOU18734.1"
                     /translation="MTSIKVPIIENILEANDFVAEENRKIFSEHNIFCINIMAAPGAG
                     KTALIVKTIELLKDKIRIGVIEGDTASVTIDSDQVVMAGAPAVQVTTAGECHMDAMMV
                     RKGMEQLPLDDLDLVIIENIGNLICPAEWDLGSHADALVASVAEGDDKPWKYPNIYRN
                     LDVLLITKTDLIPYVDYDIDHFKSGVEILNPGLVTFPTSAKTGEGFPAWTEWLLKKTK
                     AAAEK"
     gene            3519..>3915
                     /locus_tag="PK898_01485"
     CDS             3519..>3915
                     /locus_tag="PK898_01485"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOU18735.1"
                     /translation="MKSSESLHWEGRHYQVSGIVQGVGFRPYVYKLATKFGLTGDVRN
                     TTEGVEINLYGTDGQQTEFETVLRTSPPALSKVDRITICEIPFEEKKTFKIIESARKD
                     GEYLPIPPDIAICDDCRREILDPANKRYHY"
     assembly_gap    3916..4015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4016..5998
                     /gene="hypF"
                     /locus_tag="PK898_01490"
     CDS             <4016..5998
                     /gene="hypF"
                     /locus_tag="PK898_01490"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOU18736.1"
                     /translation="CDDCRREILDPANKRYHYAFNNCINCGPRFTIIKDLPYDRINTA
                     MSGFRMCPDCQSEYEDPSNRRYHAQPTGCPVCGPELEFRQQSEPAVRGERALQAAAAA
                     IRAGKIVAIKGLGGYHLACDALNRDAIRELRKRKLRSDKPFALMASDLSVIRKYAVCG
                     DAEQAALRSERAPIVLLDRLPDSPIPPEVAPKQSTLGFMLPYTPLHVLLFDAIAPDTE
                     LLIMTSANISEEPIICDETDEDMRKLFALSDCILTHNRPIITRIDDSVIRFLPEEDRP
                     ILNRRARGYAPDPIRLAEAFPQILGVGGDLKNTFCLTRDRYAFLSQHIGDMENVETSA
                     SFEQNLDHFRHLFRIRPEAIACDLHPNYFTVRFAERYAEKERIPLLRIQHHYAHIASV
                     LGEYGRFSAEPAIGLAFDGTGYGSDATIWGGEVLLCNARGFERLFHLQSFPLPGGDAA
                     VRHPARQALALLHRLEIPWTDDLPPVKALKEESVNALGVIDHQIRQRINCTETSAMGR
                     LFDAVSALIGVCQKINYEGQAAIELESIASSLPDSGYEFPPEGDEIKLKPLIESVLWD
                     FRHGISQEMISAKFHRGLIDLTVEICIKIRKKIGGNPPVAVSGGVWQNRILLEGVRNR
                     LTAEGFELLIPHQVPLNDGCIAFGQVMIAKAILGEK"
     gene            6001..6234
                     /locus_tag="PK898_01495"
     CDS             6001..6234
                     /locus_tag="PK898_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOU18737.1"
                     /translation="MCLGIPGKIINIYDEDGLRMGKVDFNGVRRETCLVGVPDAKEGE
                     YVIVHAGFAISRMDEQEALETIRALDEIDGLSC"
     gene            6218..7324
                     /gene="hypD"
                     /locus_tag="PK898_01500"
     CDS             6218..7324
                     /gene="hypD"
                     /locus_tag="PK898_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOU18738.1"
                     /translation="MDSAVDLTAFRNPERADRILKRLKNEITRPWVIMEMCGGQTHAL
                     MQSGIDQLLASQIEFIHGPGCPVCVTSLALVDEAIALAKLPNVILTTYGDMLRVPGSA
                     GNLFAARAQGCDIRVVYAALDALKIARENPDKEVVFFGIGFETTAPANGISILQAYRT
                     GIRNYSVLVSQVCVPPALEMILSSPQNRVQGFLAAGHVCAVMGTSEYIPISEKYHVPI
                     CVTGFEPIDLLAGIETVVRDLEAGCWSVSNAYSRGVPAAGTAGALAIIHELFEPCDRD
                     WRGIGIIPDSGWKLKDKYSAYDAEKKFGVTATETHESDVCIAGAILRGLKTPPECPAF
                     AASCTPEHPLGAAMVSGEGTCSAYYRYRRNHDRA"
     gene            7311..8375
                     /gene="hypE"
                     /locus_tag="PK898_01505"
     CDS             7311..8375
                     /gene="hypE"
                     /locus_tag="PK898_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOU18739.1"
                     /translation="MTELNISEGFTCPAPIQAYDRIVSGHGSGGKMTADLIEKTFRKY
                     LESDGLAQGNDAANLPIPRQVKRIAVTTDSHIVSPLFFPGGDIGALSVCGTVNDLLTS
                     GAVPQWLTAGFILEEGFPLSDLEKILASMRERSEEAGIQIVAGDTKVVEHGKGDGIFI
                     NTSGVGWIVDDFQPGGEKAQTGDTIILSGTIGDHGMAVLCARGEMGFESDIRSDVAPL
                     NEIVNSLRRAAPHTHVLRDPTRGGLSTTLNEIALQSHVSVEIDETAVPIRPEVRSICD
                     LLGFDPLYVANEGKLVIFVPEVEAQDALTAIRASRYGENAAVIGRVIHGDRSPVTLRT
                     AFGTGRILESLPGEMLPRIC"
     gene            complement(8464..9759)
                     /locus_tag="PK898_01510"
     CDS             complement(8464..9759)
                     /locus_tag="PK898_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18740.1"
                     /translation="MGKKKQMNSIGSGLNFGVDPEALDFSQDTWSWKGDIPNHPIQSD
                     GFSYDIVDENEIDRNAISLGIIGMIIIVYNAKSAKIKEEDVLPAFREVIGTRNWNTIS
                     EKEANQMAKTAISKLVDEETSGDVFDLASYAPLEIKRVLLRKLFVIARYRLDPEYRTE
                     AERRITEDIVPSIFADPQIELALLTGLIVKPKPKQEDKYTRQEEKAEESESVPSEEVS
                     EIKGEEELIIPDRDAEEALQERLKKLSKDQPVKKAKKEKPTTEKPEEEYIPFSDMEKE
                     LAGSSSPAAEPFNDGFDEFNKPPIAPAEKIPAVKTGSQTQQPKPSQVPPPPMPNIPRP
                     PIPPVPGTSHYPQTRPESSGYPSYPQPGESRSGYPAQSGPGTFPYPPQPQQPPTRGAY
                     PPPPYHAPYPQGNEMPPQQPPYTEPPAIEHTQPRKPKSR"
     gene            complement(9908..10879)
                     /locus_tag="PK898_01515"
     CDS             complement(9908..10879)
                     /locus_tag="PK898_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HOU18741.1"
                     /translation="MYGYNFAKHPVFDQKMICLWSENDHVRKTEAKLLPEFGSNLFSY
                     SVDGIEFLHQAPMPVIGSKFFGTPVLYPFPNRIRNCRFTFDGTEYVLPDNDGGRSLHG
                     LVYDTPFQFETPIVTDDDISVKTKLEILPGHPIYKLFPIANSLELTYILGADGLTIQV
                     KVSNLDQEKRFPFGFGIHPYFNVLGLKSQVKLYVPAKKWMEAVNLIPTGRLIDLDKSP
                     ADLSKPTKLSGLNLDEVWYGMEPKKPQAIIYEALGKKMVLYASDIFTHSVTYTPPFES
                     FLCVENQTNSTDCHNLYAKGFEEEAHLLILKPGESISGTIRFCVEDI"
     gene            10932..12035
                     /gene="ruvB"
                     /locus_tag="PK898_01520"
     CDS             10932..12035
                     /gene="ruvB"
                     /locus_tag="PK898_01520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOU18742.1"
                     /translation="MDKKYLSVAKRHFSMASADIIIIMDKDRLINPGIISSDDRVDLA
                     LRPKRLNEIIGQDQIRENLSILLTAAKQRNEPLDHVLFYGPPGLGKTTLAHVLANEMG
                     VSIKVTAGPAIERAGDLAAILSNLHEGDILFIDEIHRLNRAIEEILYPAMEDSVLDIM
                     VGKGPSARSIRLKLPRFTVIGATTRLSLVSSPLRARFGAVYRLDYYDQPAMEKIIENA
                     AIKMNVTVDDGGIKEIAVRSRGTPRIGLRLLRRVRDYAQVRADGTINEQVSRDALELM
                     NIDVAGLDDVDRRVLLTIIEKYNGGPVGLSTIAASISEETDTVMEVVEPYLLQLGFID
                     RTPQGRNATRKAYEHLGIPYNDQNAPKQGSLFE"
     gene            12119..13156
                     /gene="queA"
                     /locus_tag="PK898_01525"
     CDS             12119..13156
                     /gene="queA"
                     /locus_tag="PK898_01525"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOU18743.1"
                     /translation="MQTSDFDYYLPPELIAQTPLEPRDSSRLLVYNRKTDEITHCHFY
                     DIGNFLEPGDLLIRNQTRVLPARIYAKKHETGGKAEILLLRRIEPDTWEALVGGKRLH
                     EGTILDLEEGLSAEIVQDLDNSRRLIRFTGPFEDFMQKNGQMPLPPYIHKRLKDPERY
                     QTVYSRQDLAGSAAAPTAGLHFTEELMQKLEARQIHFADVTLHVGLDTFQPVSEADPQ
                     THRIHKEWCELSADTAKKITETRANGHRIVAVGTTSARTLESAAQAALKAGSPDCVIP
                     ISGDTGIYILPGYEFRAVDAMITNFHLPKSTLIMMISAFTSREKILSIYQQAIDMKYR
                     FFSFGDAMLIL"
     gene            complement(13219..13539)
                     /locus_tag="PK898_01530"
     CDS             complement(13219..13539)
                     /locus_tag="PK898_01530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU18744.1"
                     /translation="MISYKFELEKRKAKALNDDGKQIGSCTISICQDYWTIDHTFVDP
                     EFRGQNIASELVKLVVQNAREMGMKILPVCPFAQREFEKKSEYQDVLYRSAVEKPSSS
                     CPVK"
     gene            complement(13553..14818)
                     /locus_tag="PK898_01535"
     CDS             complement(13553..14818)
                     /locus_tag="PK898_01535"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOU18745.1"
                     /translation="MKTILQMGQDARKASKILATAETERKNAFLLSLADLLESDHTDI
                     LAANAEDVAEAEGVLSPAMVDRLLLNEQRIKGMAKDLRNVAELPDPVGEIFERYTNPD
                     GLKVRKERIPIGVIAVIYESRPNVTVDSAGLMIKSGNAAILRGGKETIRTNQVLISFI
                     QNALSSNGLPADAIQFIDSPDRSFMLELLQSDPYVDMVIPRGGSGLHEFCRKNSRIPV
                     ITGGIGICHLFVDESADLQKSSEVIRNAKVQRPTVCNALETVLIHRSIAEEFIPKLCA
                     KLAEDGVSFKVDGNAYNILLLNGDKSIHFEKAGENDFDTEWLSLVLSVKVVDDVKQAI
                     DHISLHGTGHSDGILSENQENIRRFTTEVNSACVYVNASTRFTDGAELGLGAEIAIST
                     QPLHARGPMALRELTTYKWVIEGEYQTRK"
     gene            complement(14815..15933)
                     /gene="proB"
                     /locus_tag="PK898_01540"
     CDS             complement(14815..15933)
                     /gene="proB"
                     /locus_tag="PK898_01540"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOU18746.1"
                     /translation="MSYERIVIKVGTSTLTGGSNHLDKPRLVDLARQLSVIAGHGTMV
                     TLVSSGAVAAGRELLNYPHLSKHIPGKQILASVGQPYLMSIYMDLFKIYNLAAAQILL
                     TRNDFIQRKSYLNARNTIEGLLGQHVIPIINENDTVSTDEIRVGDNDMLSAYVSGLIG
                     ADLLVLLTDQDGLFDMNPELHAEAKLIDLVDGVEIPSFVWEAAGGSLTGMGTGGMLTK
                     VRAADVARRMGTPVMIANGTIPDVLLRIAAGERFGTVFRATNTVLESRKRYLLTGFRS
                     NEAAIILDSGATQAIKRGKSLLPAGIIGVQGEFDRGDTVRLVNNDGEEIAVGLVNYSA
                     SEIQKICGAKASEIETILGYTYADEVVHQDNMIKGKTR"
     gene            complement(16120..17139)
                     /locus_tag="PK898_01545"
     CDS             complement(16120..17139)
                     /locus_tag="PK898_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU18747.1"
                     /translation="MEESVSPEEKNAVNEKIGEVQSAVENVADDVAVVTAADQPGDTV
                     TDIDSKGESAGANGPKIKDHFKGKVTKLSLAGALVDIGLDIPAAIHITQIARPEGAEF
                     TNNIHDLLTVGQEVDVWVKKVKDGRIELTMIKPYDLEWREIKKDMVVKGTVVRMEKFG
                     VFVEIGAERPGLIHISELAHSYVRQPSDILKVGDEVEAKVLEVNRRKKQIKLSIKALQ
                     PEPEVEEAPAEKTARPVSARAQQLKEARTTNAVSAPSEEKVRPRQQKKTSKKRAELNS
                     EGITMSFTDENPSGEAEPTAMEIAIREAMEKAKARNKSENKKKSGVSKEQDEILNRTL
                     NNKVG"
     gene            complement(17271..18593)
                     /locus_tag="PK898_01550"
     CDS             complement(17271..18593)
                     /locus_tag="PK898_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU18748.1"
                     /translation="MNQRIIRIIAIVIILISCFVSGFSAGYIIKMLPETVGKINISSE
                     SSLLPKLLSVTEQGTGMKRQQNTDELFKPFWETWDLVHQYYVDQPVDDNSLMEGAIRG
                     MLESLGDPHTRYSDPESYKQESQYIAGEEYEGIGAWVDVSGDYVEITSPMKGSPAEKA
                     GLKPHDLVIAIDGVDMTGVDPKDALNKILGPKGSTVVLTIKRDDQEPFDVSIVRDAVV
                     TPMVASEMRDDGIAYIQLTQFGDLTVEELQQALDELLPQNPKGLILDLRNNGGGYVDT
                     CVSVASKFLPKDSLVLIEEAGDGSRTEYHTKEDPVVDPDLPIVVLGNEGTASASEILI
                     GALQYYHRATFVGVQTFGKGSMQIQPELSNGGAVSITIAHWLTPAGKLIHKVGITPDV
                     VVEMSDADVEAGKDPQLDAAAQILLTGKIPEEPSVPQKNTEENGETGL"
     gene            complement(18590..18871)
                     /locus_tag="PK898_01555"
     CDS             complement(18590..18871)
                     /locus_tag="PK898_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18749.1"
                     /translation="MTTKGWIILIALIVVVMGINILLIVYREKIKAYFEKRRKPASKS
                     NQDDSFSKLTKKVKEVKKGDFLFPESEHYKRLRQEMDRLENLERDKDQK"
     gene            complement(18868..19515)
                     /locus_tag="PK898_01560"
     CDS             complement(18868..19515)
                     /locus_tag="PK898_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOU18750.1"
                     /translation="MNYKAVFFDLDETLYPMGNESIKVITDRMNEYIRRSLAIPDSEV
                     KNFREDLYKTYGTTAKGLVKNYEVDLYDFMHYVHNVDISPYIQPAPEVRALIEKIQIP
                     KFIFTNSDRFHAIRVLKLLDNYQCFDGIIDFLDVYPYCKPEQEAFEKALAMTGIDSGR
                     GCIFLDDNPTNISTAHQLGFYTIQVGSRARSEEADAFLSRIEDLPQLEIYSEIIT"
     gene            complement(19585..20352)
                     /locus_tag="PK898_01565"
     CDS             complement(19585..20352)
                     /locus_tag="PK898_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18751.1"
                     /translation="MISLSDVSVHYGDQPVVQNISFDIRQGKVMVLIGPNGSGKTTII
                     RGITGSVKLSGGQIKYDGKNSADLSETERAKYLSVVPQNSLLPDGFSVYETVAMGRTP
                     YLGLLGKLSEEDRRRVDEAIRETAIFDLRKKDVRSLSGGEQQRVILARALAQDTPVMI
                     LDEPTTHLDLNHTIGILSLVRNLSREKGTAVLIVLHDLNLAARFADEIIILKQGNILA
                     MGTPEEVLTENNLTAVYHVPMIVFRHDGISTPIVLPK"
     gene            complement(20349..21326)
                     /locus_tag="PK898_01570"
     CDS             complement(20349..21326)
                     /locus_tag="PK898_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU18752.1"
                     /translation="MSIAGIWVGSVPLGISQVWDILKYRITGGDLPAELAGTDRILFV
                     LRIPRTIMMLLTGAALACSGASYQGLFRNPLADPYLIGVSTGASLGAVIAMSVQWPYT
                     KWGTMMTPILAFVVSLLTVWVVFKLGKVSGITGNTGLILSGVAVNSFITAVTSVWMLF
                     SHRELRTSFSWMLGGMTVNGWEPIKIVIPFILIGIIGQFLLSHRLNVLQFGEEQAANL
                     GISVHKTRIGIVFFATLTTAAAISFSGVIGFVGLIIPHVIRILTGGDYRKLIPLSALG
                     GAFFLLLADVIARVIIAPQELPVGIITAIAGAPFFLWLLNRNTGREIER"
     gene            complement(21398..22336)
                     /locus_tag="PK898_01575"
     CDS             complement(21398..22336)
                     /locus_tag="PK898_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOU18753.1"
                     /translation="MRKKTLFYCVVFLTILFSIAMPVSAVRDTDVAEKITVTDDLDRK
                     ITLELPLESVVSLAPSVTEIVCALDLCDELIGVDVASNYPDEVTKKAIVTNYDMSINK
                     ELILSLDPELVIISELTAVDQVKQLEDMGLTVYYLKNPKTLDDLDSYFMKVGEVTGHE
                     EQAEELIAEMKNRIAVVDETIAGATTHPLVFYELDATDVSKPWTASSGTFIDELITRA
                     GGKNVAADLKGEWVQISLEALIRSNPEIIILGDSNYGITPESVIERPGWDVLSAVKNK
                     KIYPINSDIGSRPSPRLVDALEILAGYIHPELFEKK"
     regulatory      complement(22493..22620)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(22632..24071)
                     /locus_tag="PK898_01580"
     CDS             complement(22632..24071)
                     /locus_tag="PK898_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU18754.1"
                     /translation="MESIDDIIERVKKDFNVTPKNPKSLDDMNREAYLGDPNCPICHG
                     IGFVTGDYPIDDPRFGKMDVCSCRKAKLEEETRNQLIRESNLSGYADMSFETFNVEGR
                     GQLRPDERLCLKLAFDHAQKFAKERNGWLLLTGNYGTGKTHLAAAISNAALADHIPNI
                     FLPVPDLLDWIRASFSDSSGTYAERFDQIRNIPLLILDDLGTQSATPWAEEKLYQILN
                     YRYVNRLPTVITTNCRLQDLDGRIASRLEDPSLVTRIVIQAPDYRKPMANTVESDDLS
                     ALHRLSNRTFENFDSRVGENLSEEASAQLTNAFNLAYNFAQHPTGWLVFSGPNGVGKT
                     HLAAAIGNYRQHQMDHPLFVVVADLLDHLRATFSPVSTTSYDIAFEKIRNAPLLILDH
                     LDTANATPWAKEKLFQILDHRYLTPLPTVITTVLPIQDIDPNIRSRLVDFQVCQIAQM
                     FQVPMYSRNPQVSILPERTRKGYPSRRGK"
     gene            complement(24058..24672)
                     /locus_tag="PK898_01585"
     CDS             complement(24058..24672)
                     /locus_tag="PK898_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOU18755.1"
                     /translation="MSFSGFNTGIEVIPTQFFEEILPEIDSMTELKVSLFAIHLLNQF
                     EGDQRYILYSDFTDAAEFMDGLAGIEEDREDALKDGLEKAVLRGTLLKVEYGGESLYF
                     LNSPKGRMAVENLEKGKWKPDAFLHLSGTLNLFRPNIFQLYEENIGPLTPLMADRLKD
                     AEQMYSEEWITEAIDIAVVNNARSWRYIETILKSWKENGRNGIY"
     gene            complement(24669..26066)
                     /gene="dnaB"
                     /locus_tag="PK898_01590"
     CDS             complement(24669..26066)
                     /gene="dnaB"
                     /locus_tag="PK898_01590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOU18756.1"
                     /translation="MEINGPYPTENFTPEVSESDLRLQPHSREAEEAVLGAILINSEK
                     FYDVAQIISANDFYIHRCRWIWEAFAALLEKGQDIDVQTVANELEKNGKLTEAGGKLY
                     LYSLASNTPSSMHAESYAKIIAEKSVRRQILTAASQMQQYAYDESKSLDTILDNSEKA
                     IFNVSEKRTRNEVQPIRSVVGTIYDEVRLLARQDQDIVGVPTGFKDLDRLLGGMQKSD
                     LIIVAGRPGMGKTGFLLSVLKNAALNYHMHVAMFSLEMSNEQLAIRLIAQQTGINSQK
                     IRSGKLAEDELPMFIDALDVLGTTTVFLDDTPAITPLQLRSKCRRLDLEHHLDLVIVD
                     YLQLMSTDSRNENRVQEVSYISRNLKILARELNVPVLTAAQLSRSVEQRSEKIPILSD
                     LRESGSLEQDADVVMFLHNPNTDDSKTVGLDGAMKLVVAKHRNGPVGDIDLVFVKKLA
                     RFENAARISDSGGIR"
     gene            complement(26070..26699)
                     /locus_tag="PK898_01595"
     CDS             complement(26070..26699)
                     /locus_tag="PK898_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOU18757.1"
                     /translation="MSELFEVTVDSIRVSLTTQQRVLILHEPKNELYLPIWIGSFEAE
                     SIVIALQGIEVSRPQPHDLLKNVIAALDGKLRNVVLSKIQDDTFYSFLVLESQGNEIL
                     VDSRPSDAISLAIRCKVPIFVDKTVLLSAGIQPEKDIRELNSAKKNFAAEPDQEEFVD
                     DAKLNIFDDFLKNIDLPDSGETGKSDRSGKQDNEKKDTDSNSDDLLFPI"
     gene            27002..27739
                     /locus_tag="PK898_01600"
     CDS             27002..27739
                     /locus_tag="PK898_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU18758.1"
                     /translation="MDQLQDKIILIEGKQTDHPSFFLSLKKRGYNVEIASTGNSAIEK
                     IQESDPMIVLIDAASMRTASNRIVYRIKKRNPDLPIILIIDEMLKQKKISDKADLVLC
                     LPFTVQKLLNRIMLFAPAKKSTLKCAGDLALDTSRNCIYIDNRVVGVTPRVSKLLNLF
                     MDRPGEIIKREELFRELWDTDYLEDMRSLDVHIQWLRKAIEENPKNPQYLITVKGQGY
                     MLEPNGNGKNKKIDQCALSPVCCEKES"
     gene            28066..29343
                     /gene="serS"
                     /locus_tag="PK898_01605"
     CDS             28066..29343
                     /gene="serS"
                     /locus_tag="PK898_01605"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOU18759.1"
                     /translation="MLDMNLIRENPELVIKSMKDRQMDPAPVNRVIELDMTRRKLLSE
                     SEQLKAERNATSKEIGRIKDAEERNAKIEAMRLVGDKIAELDKKVKETEEELNSLIMI
                     IPNIPDPRVPYGVSDAENVVVRYEGKIAEREFDPIPHWDLGPALGIIDFDRGVKLTGS
                     RFYILNGAGARLQRALIAFMLDLHIRQGYEERYTPFMVKSEILYGSGQLPKFRDNLYH
                     DAEEDFWMVPTAEVPLTGLYMNEIIEEDSLPMRFTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYCYSHPDKSADELDRMLNAAEETCRQLDLPYRIVAQCTGDLGFNARICYD
                     IEVWAPGCKEWLEVSSVSDDGDFQSRRGAIRFRDKESGKTRFLHTLNGSGLGLPRTLI
                     AVLETYQQAGGSVVVPEVLKPWMGGIDVIEKQQ"
     gene            complement(29394..31154)
                     /gene="argS"
                     /locus_tag="PK898_01610"
     CDS             complement(29394..31154)
                     /gene="argS"
                     /locus_tag="PK898_01610"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU18760.1"
                     /translation="MFEKEQQLIEDQIRKICEAHNYPVKSLTWSWIPFSGNWGISTSF
                     FQVAADDARSRGQKINVGLHAQEIAQMTADSLELPEGFERAEAVRGYLNLYFTTRIYS
                     QRVIDTVLEEKDNFGRGQRNGERVMVEFSQPNTHKAFHVGHLRNVILGNAVCNILDFA
                     GNDVIRANYLGDIGLHVIKWLWCYLKYHNGEKPEGDITRWMGDIYAEASRRHEEEPEA
                     ESEIRELFARWDRKDPEIVELWRVTRQWSLDGFEQIYKTLNVRFDKLYFESDVEDSGK
                     EIVKELIAKGIAVDERPENAVVVKLDELCGTKDKYRSLVVLRSDGTSLYATKDLSLAI
                     RKFEEFHLTRSIYVIDVRQTLYLQQIFKTLELMGYSWAKNCYHLAYEIVNLPGNVTMS
                     SRDGTVVFLQDLIDEAKSRALAIVQEKNPSLTDAEKAEISEKVALGALKYSMLSRDNA
                     KIVTFDWEAALDFNGQAAPYIQYAAVRANSILRKSENGLPDQTALSYELTPVEVELID
                     LISRVPREIQRSAAECKPLFISNLAFDLAQAFNNFYHQCPVLQAEPPVRDYRLRLAAA
                     ARQTMINCLALLGIDSPDIM"
     gene            complement(31307..31573)
                     /gene="rpsT"
                     /locus_tag="PK898_01615"
     CDS             complement(31307..31573)
                     /gene="rpsT"
                     /locus_tag="PK898_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOU18761.1"
                     /translation="MANIKSQIKRNRQNEKRRIQNRVVRGSTRTALYKARLAVETNQE
                     NSEEMVKKAVIALDKAAQKGVIHKNNAARKKSRLIKKLTATKKA"
     gene            complement(31710..32522)
                     /gene="lgt"
                     /locus_tag="PK898_01620"
     CDS             complement(31710..32522)
                     /gene="lgt"
                     /locus_tag="PK898_01620"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU18762.1"
                     /translation="MEFYSYGFVIGPFLIHYYSLCFLAGVFSATLLASRTGKKRGIAP
                     DTIWSCLFWLLIAGIIGARLWHVFLPSKSSGLTLSYYLHNPVKILQVWNGGLGIPGAI
                     LGGYIGLWLFCRKYGFKISKFTDTISPGLALGQAIGRLGNYFNQELYGAPSDLPWAIT
                     IDPQYRIPGFEQQATYHPLFLYELIYNLLNMGFLLWVDHRFGKRLKDGDIFLIYLIVY
                     PVGRFFLEFLRLDTAMVGGLDMNQLIMGVTAVCATAGLIIRHTFQPKRDLTK"
     gene            complement(32550..33188)
                     /locus_tag="PK898_01625"
     CDS             complement(32550..33188)
                     /locus_tag="PK898_01625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU18763.1"
                     /translation="MNENISNNSSNQPEKPKTFSDQCRIWFRGFGETCSRFLLKIGLT
                     ANTVTIIGCAGHIIAAYLAATGHFTWAGILLVFFAVTDFFDGTMARLSTGGKGTRFGA
                     VLDSCTDRYAEFFIFGGLIYYYAVHEDYLTMMVAYIAIMGSILVSYTRAKGEIERLDM
                     KLGIMSRLERYLFLVPCLLFNIPFIAMWAIAIGSHITAIQRIQYMRKELKEN"
     gene            complement(33208..35382)
                     /locus_tag="PK898_01630"
     CDS             complement(33208..35382)
                     /locus_tag="PK898_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU18764.1"
                     /translation="MPEKENKQENEPQKPSGSRLKSESLIPDILALGCIVTGLLLWVG
                     FTGKTDGRFILRMRNFFDDWIGTGRIIFVVLFLGLGTGIFLSRYTTVKIRIPLKKIGL
                     IILLFFLLLALVSSSDHFSGGKIGDTLFGQLTAVIGEIPATILLWGIIAVLFYFLFDL
                     NRPLSESAVFIKKKVAHFCASKPIRSSDEVTLFRRRLMKENIEKEENDPLEKNITNHP
                     IDSVIGGNEIKGKSKPRTNRLPPINLLEPEMGFLNKNIDVQSRIDEIESAMEEFGTPV
                     KVIGYSIGPAIIQYQVVPGALEKNGSHGTVIPKKIRVAQVAQMERDLAVRMGVANLSI
                     QAPVPGENFIGIDIPNPDSLKVRLRPLMESREFQSKTSPLNVALGRDISGKPVVVDLE
                     QMPHLLIAGTTNSGKSILIRSMAVCLIMNNTPETLRLIMIDPKRVELFRFNGLPHLLG
                     KVETEYDRSISVLGWAVQEMKNRYKLFETVGVRKLSLYNQYAVRNGQKPLPYIVIFID
                     ELAEIVKGADKLGQEYIDTLASLARATGMHLVVATQRPDTTVITGKIKTNIPARIALN
                     VASAIDSRVIMGKQGAEKLLGRGDMYFVDPSLNVPIRIQGPLLTDQEIDSVVALWKKL
                     SPKPVEEAEAPWEEMIEDAEKEVNGKEEKDLKAAIRLVCRTRKASASYLQGKMNISFP
                     KASRLLDRMEQIGVVGPIQVGGKAREVLWSEEEADSYGENAA"
     gene            complement(35439..37331)
                     /locus_tag="PK898_01635"
     CDS             complement(35439..37331)
                     /locus_tag="PK898_01635"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOU18765.1"
                     /translation="MINWILPIIRTISEILTASIAIASFSLLLFSLFFIQKERLARVF
                     SLILLALSVIYTADALSLTTLSDQNQLLWQQIHWSGIIFLPATMIHFSQVILQMTGKT
                     IRKLRKKMTAVTYIFSVIFLVLLWKNQLFYGTRRVGNLGISMIPTEKLNYFWIWLFVL
                     LSAMLINFYHAFKRTRTPTSRRRMLYLIVSALGVLVGTFPLMIYGSGFLLRHELGFWL
                     VNLIANILILLMVLLLGYSVITFCVSWSYRVVRLRLMEWILRGPVTAGLTLGLVTIIR
                     RSSGTLNINVGGWTSLLMVVSIVVLEYLITLLMPLFERNSISGYGNQDYQIVSGLENM
                     MIFSSELETYLESIISALCDIYQAKGVFVAVTDSMGNVENLIHVGETSLSDPIVFSEK
                     IEALQGFTDEPITDDGSILLPVFYHDLTENKTWLLAIIGITDPAVTGEESEPQSALKV
                     AADKIRIVLWQRWYLGRTFNVLRELSNDEKLGSYRKIGLLNTAEVLDQKSDYELEEVS
                     TWVRDALTHYWGGPRLTENPLIRLDIVQQEMNGDSNNATNALRTILKKAIDQIRPSGE
                     RSISGEWTLYNILDLKFIEGLKVKEVAKKLAMSEADLYRKQRVAIEELAKTIIRMEKE
                     HNSSHL"
     gene            complement(37344..37883)
                     /locus_tag="PK898_01640"
     CDS             complement(37344..37883)
                     /locus_tag="PK898_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU18766.1"
                     /translation="MAEIEIRMASMADIPLITKIDLDFESNYVWKSQMIDDLDSYTIV
                     FQNIKLPKTVHVPFQGGGEEAIEKMVKRQEVIVAQYDNQVAGFVRLEQDENTNRILLK
                     TGGVKPDYRRKGIGSALLKVIQENAVKVGIPRLVCTVQAKNDPAIHFLLSKGFQFCGY
                     QDFYFQNMEIALFFAKNIR"
     gene            complement(37942..38637)
                     /locus_tag="PK898_01645"
     CDS             complement(37942..38637)
                     /locus_tag="PK898_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18767.1"
                     /translation="MQSLLNFLVNYQNWIYTVLLITAVIYGRKLIRAIIEWRSTIFGL
                     EREFAQHKLNTSLAMVILSVLLIIAEFICVNFIISALPQATGIQSLPATTDGVDLNIV
                     ATSGTGVETGSPNASSAVAQNVTGGGCIPGQIEWIAPKPGEEISGIYNLMATVNILDM
                     GFFKYDYASLSDPTDWHAISAGNMPVVEGKLGVLATSEIPNGDYILRLTVVNKANQQL
                     TPCDVAIRILNKE"
     gene            complement(38656..39489)
                     /locus_tag="PK898_01650"
     CDS             complement(38656..39489)
                     /locus_tag="PK898_01650"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU18768.1"
                     /translation="MKTYEYADYVKAAEIIKEKIKTPIELALVLGSGLGGLADEVKHP
                     LIIDYRDLPGWPLSTVEGHKGRLVIGELAGRMVLVQQGRVHFYEGYSPAEITFPIRVM
                     QCLKIPSLIVTNAAGGVNPSFSAGDVMLITDHIGFVSMSGFNPLNGRNFDQFGPRFPD
                     MSQAYDREYGSLVRKAAIRTGIDLKEGVYTWLSGPSFETPAEIRFLRTVGTDAVGMST
                     VPEVIVARHGGMRVLGLSGITNKCSDDGEMKADHQEVLQIADIIGPKIIRILYEILPD
                     M"
     gene            complement(39707..42388)
                     /locus_tag="PK898_01655"
     CDS             complement(39707..42388)
                     /locus_tag="PK898_01655"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOU18769.1"
                     /translation="MDYYLIGTDEVFKKVSSSPQGLTQAEAEKRLSEYGRNVLVQPPK
                     PTIWQRLLEQVSDPMVIMLIVAAVISGITSTIRGESIADVFIIMAVVVINSVLGVYQE
                     NKAEEAIEALQKMTAATSKVLRDGRKVVIPSSELVPGDVIILEAGDAVPADGRIISSA
                     SLKIEESSLTGESEPVSKFIDILHLENSDTVPLGDRKNMCYMGSTVVYGHGEAVVTAT
                     GMNTEMGKIAGALGEAENELTPLQRKLNDLSGILTKLVIGICVFIFIFTLIRNGNFSG
                     PVILDTFMIAVSLAVAAIPEGLVAVVTIVLSVGVTNMSRRNAVIRKLTAVETLGSAQV
                     ICSDKTGTLTQNKMTVVDTFGDDMQLLSIAMALCNDAELETSPEDLNNPEKIEVSGEP
                     TEAALVIYAAKNGLDKNKLKKEYPRIGEAPFDSKRKMMSTVHRHDGKIIQFTKGAPDE
                     VLTKCTKWLHNGSVIPLTDELRNEIMEANNAMAANALRVLAGAMKEYPAEPKSYEAEE
                     IENDLTFLGLMGMIDPIRPEVIGAIKECSEAGITPIMITGDHKATAIAIAKQLGILES
                     EDQAITGAQLDHISDEEFEKMIENIKVYARVQPEHKTRIVKMWKKKGMVTAMTGDGVN
                     DAPSIKSADIGVGMGITGTDVTKNVADMVLADDNFATIVSAVEEGRRVYDNILKVIQF
                     LLSSNLAEVIAIFVATLIGFQILRPVHILWINLITDSIPALALGVEKAEGDLMKRKPR
                     DPNSHIFADGMGFDIGYQGVMIAIVTLVSYFVGHYLEAGRWEITQSPDGVTMAFLTLS
                     MAEIFHSINMRSRRNSIFSMHSINKFLILAMVIALILTTGVIEIPSIAALFEFETISL
                     NEYMTAMALAISVIPIVEIVKYFQRKRNSNYNGTVNK"
     gene            complement(42647..43588)
                     /locus_tag="PK898_01660"
     CDS             complement(42647..43588)
                     /locus_tag="PK898_01660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU18770.1"
                     /translation="MDHGLQSSGKTLRFQYRNSELMRLDHYLVEVLPDYSRSRLQQFI
                     TEGRISVDGKPAGKSGLKLNSGQEIEITLPEEKTSELIPENIPLDILYEDAQVIVVNK
                     PAGMVVHPSAGHESGTLVHAVLAHCQDLKGFGGEIRPGIVHRLDRDTSGVIIIAKNEQ
                     AHIFLQKQFKSHKIDKRYLALVDGKPPTPNGRIETPIGRDPIHRQRMAVLPAEKGRDS
                     VTEYFTKESFPNHTLIEAHPLTGRTHQIRVHMAFLKCPIVGDVLYGYRKKSVPIHRHF
                     LHAARLTILLPGKEFPMTFEAPLPPELEDVLNDLRSA"
     gene            complement(43590..44219)
                     /gene="lspA"
                     /locus_tag="PK898_01665"
     CDS             complement(43590..44219)
                     /gene="lspA"
                     /locus_tag="PK898_01665"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOU18771.1"
                     /translation="MNKRGKVYLKLFSIALGVIFIDQLTKWFVRTNLDIRETWMPISW
                     LEPYFRFIHWKNTGVAFGLFQGMGWILTSLGLVVILIIIFFYKPVFNGPAFWQIALGL
                     QLGGAIGNLLDRINPNVGYVVDFIWIGNFPVFNLADFAIVSGAIVMLIGIWYTEERAE
                     KKEGNKEGKSFETSSPIGSITDNTSRVPADPDVSNGSQIPELSRDGKQP"
     gene            complement(44397..45113)
                     /locus_tag="PK898_01670"
     CDS             complement(44397..45113)
                     /locus_tag="PK898_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU18772.1"
                     /translation="MKKNSLLMIVFLSMMILSLGMAGSVQAQSDQTMTVSGTGTVLLK
                     PDIAIINVSISTEKESVQEALDENTKSVKLVKSALTEMGIPEEDIKITSYSLYSTQKY
                     YRDETAGMDEYIFSVFYGFEIKLRDISKLNDVLDACINNGVNSINSVSYDSSLREDTY
                     MQARDLAIDDANANAQQIAQKLGVTVGNIKSFQVRDYPTDYGAMYGMGGGGYGGTAPL
                     ESGSLKIVVTVDITYEFSLP"
     gene            complement(45164..45874)
                     /locus_tag="PK898_01675"
     CDS             complement(45164..45874)
                     /locus_tag="PK898_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU18773.1"
                     /translation="MNFKKILFLFIGTIALFGISVFKVSAQNESPAIIKVKGTGEVTL
                     NPDLALIDIAIATTADDSKTAIDENRKNALAIQQGLLEMGLKEDDIRTTEYILYSLSK
                     TDKDGNPIAGQYIYKVNLAYEIVVRDLSMLNDVLNFCIQNGANNLYGVNFDYSKRSEA
                     VAQARDLAIDHANVNAQQIAEKLGKKVDHLLTFSVLDNTEIPLISTKLVKDVSTAPID
                     SGFLTVTVSVDMEFALTD"
     gene            46181..47239
                     /gene="prfA"
                     /locus_tag="PK898_01680"
     CDS             46181..47239
                     /gene="prfA"
                     /locus_tag="PK898_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOU18774.1"
                     /translation="MLDKIDAIEARYNELSNLIAENVEDYQKTAEWMKERSEIEEIVE
                     KGREYKDTLKRIEEDKALEKDPEMSELAATELDDLLPKKENLENEIRSMLLPKDPRDS
                     RNVIMEIRAGTGGDEAAIFAADLFRMYSHYAEARHWHIEVMSANEIGLGGYKEIVFMV
                     KGKGAYSRLKYESGIHRVQRVPITESQGRIHTSAVSVAVLAEVEDVDIQIPESDIRIE
                     VYKSAGAGGQNVQKNSTAVRLIHLPTGMVITCQDERSQLQNKTRAMSILRARLYDMEQ
                     QKRQEQLDATRRSMVGTGDRSEKIRTYNYPQSRVTDHRINVSSFNLAGVMDGDLDVFI
                     DELIMREQTEKLSVSEGA"
     gene            47236..48099
                     /gene="prmC"
                     /locus_tag="PK898_01685"
     CDS             47236..48099
                     /gene="prmC"
                     /locus_tag="PK898_01685"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU18775.1"
                     /translation="MNGSDSVGEWLDWARRELQTSGYSDTPSIEAQWIFEKVTGAHRP
                     ELLSHPEKPLSDTEITKIRDMVEKVKEGVPLPYVLGEWSFFGNTFQITPDVLIPRPET
                     EILVEQAIEWAKDRLMPIRFADVGTGCGCIAVSLLNYFKSARFTGAAIDISDAALRVA
                     RRNIDHYRLNEVLFPIRGNLTECLSSPLDLVCANLPYIPSERCRSLDVAQNEPLLALD
                     GGEDGFDLYRMLLRDLTGKMAKESLILCEIDFSQKREALSLSRQLFPDASVTIKEDYA
                     GLSRLLMIRKS"
     gene            48102..48737
                     /locus_tag="PK898_01690"
     CDS             48102..48737
                     /locus_tag="PK898_01690"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOU18776.1"
                     /translation="MKKIYDITSPQIQPVAIQQAIDVLNSGGVVIFPTDTVYGLAASA
                     FNEGAIQRLYRMKERDPQKSIAVLLGNISQIRLIAADFPESARKLAERYWPGGLTLLV
                     SKLDTLPPALSANEKIGIRIPDYPVIQDLIIHTGPLATTSANLSGEPPAKKLSDLPDS
                     LLRQADLILDGGPVKGGIASTVVDCTVRPLLVLREGAISKEEVAAFDSETE"
     gene            48734..49891
                     /locus_tag="PK898_01695"
     CDS             48734..49891
                     /locus_tag="PK898_01695"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU18777.1"
                     /translation="MNPKQSGIPMRLRIWIVLCMLIISGCSKTDSHRPLSGGITDLFT
                     LKTVTMPALATSRPTKTATPTPTSEKSLTATITPTETSTPLPIVTPTSFFVDISAYPF
                     SASDKLEMVDDITIPDGTMLKPGQMFIKSWRIKNVGNKIWNNNYRLALAYSNPFNTPQ
                     MTHAIFIQPTELIDFAISTWNPRQYNVGVNDTVDLVIPLQAPEQAGDYMVEFFLINSD
                     DEIVPPKIWIRFSVELLPQFATQTAVAIAELTETATPPSERVRVPTPTPQPFDWTGTW
                     LIRNPYSDTGMIPVQAWLTQSGDTVTGFFYDTDEEPVLIEGTVDRENQGFIGKFAQPW
                     QNRAVPVKWRMLINRNQFYAVTDSGETEFGTICGGRNGMAFPEHCSMPPGI"
     gene            49904..50902
                     /locus_tag="PK898_01700"
     CDS             49904..50902
                     /locus_tag="PK898_01700"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOU18778.1"
                     /translation="MIWIPNENTDPAFNLAAEEVILSSLNIKEETLFFYINEPSIIIG
                     RHQNTREEINAEFVGEQRIHIVRRLSGGGAVYHDFGNLCYSFIYPFPDRLTPDFALFT
                     APIVQALQNLGVPAELSGRNDILVAGAKISGNAYYHNRFGSVCHGTLLFDSDMGMLER
                     ALRPKPEKIERKGVRSVRSRVTNLKDYLPDIKDVKELRQAILYSISRTQPDFKEFRLA
                     ENDILKIESLAESRYRSDRWNFGKSPKYNYRVCRQFSFGEVDARLWVEDNRIREIRFF
                     GDFFSSEDPDSFSEKLIGIPFESQAIAQVAQQSDIEKLFPGISAFEFADFLTGKRS"
     gene            complement(50971..>51464)
                     /locus_tag="PK898_01705"
     CDS             complement(50971..>51464)
                     /locus_tag="PK898_01705"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU18779.1"
                     /translation="PIGCGQTISQPSLVAQMIELSELKPGDKVLEVGTGSGYQTALLA
                     ELGYGDVFSVEIIPQLYERAKKILNELGYTNVKLKLGDGYYGWPEYAPYDAIVVTAAA
                     EKVPPPLIEQLDEGGILLIPIGPRNRSQTLWKLVKENGKLTRKDVGNVIFVPFVGDGI
                     RND"
     assembly_gap    51465..51564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<51565..51911)
                     /locus_tag="PK898_01710"
     CDS             complement(<51565..51911)
                     /locus_tag="PK898_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18780.1"
                     /translation="MKSRFHLCENAEKLRNICLTFYAIPLHFIISKCCLNSKEKNSHC
                     EEDPYKKDREQLFDFAAKYMNLKNPDILRACKIVPRHKFVPSREADQAYDDCALPIGC
                     GQTISQPSLVAQM"
     gene            complement(52060..52920)
                     /locus_tag="PK898_01715"
     CDS             complement(52060..52920)
                     /locus_tag="PK898_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOU18781.1"
                     /translation="MLVTLKDVLEIAEKRTCAIGGFNGPNLASILGVLSAAEELNAPV
                     ILMHAQSHESYMPLEVVGPILLREAERSSVPVCVHLDHGVDIPYLEKALNLGFTSVMY
                     DGSTLPFEENIANTQKVIRLAAAKGASVEAEIGVMHGAEGSVDHDEDPASVYTDPESA
                     KEFVERTGIDALACSFGTVHGIYLKEPKLDFDRVAKIRKLTGKPIVMHGGSGVSHDDY
                     RKVIARGVRKINYYTYMARAGADNVKKHLAEAKGTVLFEHIESWAMEAIHDDVKKTIS
                     VFWESQTPVV"
     gene            complement(52991..53719)
                     /locus_tag="PK898_01720"
     CDS             complement(52991..53719)
                     /locus_tag="PK898_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOU18782.1"
                     /translation="MKTTIVIPTYNEYENLPLLVERIFALPMDNLHLLIVDDNSPDGT
                     GKLADELAVRYDNRIKCMHRSGKLGLGTAYNQGFRMAMADGADYIGQMDADFSHPIEK
                     IPVMAAALKDCDVVIGSRYVKGGKLDESWPFYRKWLSGFGNFYARAILGLSIRDVTGG
                     FKLWKRETLEAMPLDRIKANGYMFQVEMNYVAAKLGFKFIEIPIYFRERMAGTSKMNL
                     AISREAATRTWALRRMYKDLKPIK"
     gene            53896..55098
                     /locus_tag="PK898_01725"
     CDS             53896..55098
                     /locus_tag="PK898_01725"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOU18783.1"
                     /translation="MRSLSKIITTILSAIVACNAVSIPVFSQNGQTDPIPVEVNQTGS
                     ASAEFLCRPDNYSMNQDYCVSLGPSEKLTKLAVEGKSGAKVPFNPQKIDPSLSTVPYN
                     YAKLNLENTESAPLYNTVEDAIAGINAIGEIPAGGIRYVSYINRVDTADGHYVQAKNG
                     SWLRASPAALPTATLGRTFTATPSNNFGWVFEGCNPYLEPNYNSGVNTSVWFDREQVL
                     EVLETTQDKNTAWFKVGENQWLDRNHFRCAYINTTPPEGVTNGRWIEVDLHEQVVMAY
                     ENNSLVFAAMVATGMKPFYTKPGIFQIYEKKEAENMTGAFEADKSDYYSLDDVPFTMY
                     FDEARALHGAYWRAWYGYEQSHGCVNMTIGDAHWLYNWAQIGDTVYVHDPSGETPTDP
                     SYYGAGGA"
     gene            55107..56537
                     /locus_tag="PK898_01730"
     CDS             55107..56537
                     /locus_tag="PK898_01730"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOU18784.1"
                     /translation="MAEDDSYRIERDSLGEVRVPKQALYGAQTQRAVENFRISGLKPW
                     PVFITSVVLIKKSAAIVNADLGLLPTDYAEAIEQAADEILNGMWLDQFVVDPFQAGAG
                     TSHNMNANEVLANRANQILGKECIHPNDHVNMAQSTNDVIPTAIRIGCLLRLNELTDA
                     LENLYTALRQKADEFNTVLKSGRTHLQDAVPVTLGQEFGAYADAILKDTDRIRRSADT
                     LAELGIGGTAVGSGLNAHPDYHRKMIAILGELTGIKFRTSENLFERMQSMADMADFSS
                     AMRTTALSLIRIANDFRLLSSGPGTGLDELHLPALQPGSSIMPGKVNPVMAEMLDMAM
                     FHVIGCDTTVGLASQAGQLELNVMMPVIAHNLFEMMQIMIGSINAFSEKCVKGSQANS
                     EKAAHWLDQNAIIVTVLNPLIGYAAGAALVKEAVEKKVPIRRLAHEKAGAGLLKRLDN
                     GQPVSPAEIDHAFSDLLRMTQGGFVR"
     gene            complement(56562..56924)
                     /locus_tag="PK898_01735"
     CDS             complement(56562..56924)
                     /locus_tag="PK898_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU18785.1"
                     /translation="MPLYDYFCKNCGKKFEIRISYEDYGKMDVICPFCRSTFVERAIQ
                     PVSVVQSDQFRMVQEADSDSFNEPYDDPRELGKMMRSFKDQSGEPMAPEFDEVAERLE
                     RGDSIDSIDQDFADMDID"
     gene            57157..57834
                     /gene="gmk"
                     /locus_tag="PK898_01740"
     CDS             57157..57834
                     /gene="gmk"
                     /locus_tag="PK898_01740"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOU18786.1"
                     /translation="MEWFWDELAKNHRDIADMELSLPSFKILKSDPMIVIISGLSGSG
                     KDSVINALRNNSKLNFHFVVTCNTRKQRDDEIDGVHYHFISREKFLDMIRKNEMIEYS
                     PVYNDYKGVPKFEIEKGIAKGQDMILRLDPQGMKKVKQVYPNAVTIFIVPPDPETWLE
                     RLIKRGSDTDEDLRVRIETAREEIKSIPEFDYIVVNDQLDKAVEDVLTILRAEHQRTT
                     RIKVEHN"
     gene            57837..58679
                     /locus_tag="PK898_01745"
     CDS             57837..58679
                     /locus_tag="PK898_01745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU18787.1"
                     /translation="MNRLAVDSAAFPEEHRFNSDLFNSEPEKIPAEPDTLSEEDRQEA
                     SRRVPVRIPNAKPIVTWILCGVTVIIYLIQVGTQQFMGTDLPLALGAKYGPLIRLGQW
                     WRLFTPIFLHGSILHILFNMYALIILGREIEPAYGHRDFLIFYLITGFAGNVFSFVFS
                     PQTVSVGASTSLFGLIAAQGFLIYHNKKFIRNYQRAINNVIFVVVINLVLGMQSGIDN
                     WGHLGGLISGVIIAFLAGPAWDIRINSSQNGLELYDKVTPSKRLIAFLLTFLLFASLA
                     FAFR"
     gene            58688..59509
                     /gene="uppP"
                     /locus_tag="PK898_01750"
     CDS             58688..59509
                     /gene="uppP"
                     /locus_tag="PK898_01750"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOU18788.1"
                     /translation="MNFLQALILGVVQGLTEFLPVSSSAHLALVPYWFQWNLSEESVF
                     LFGVLVQMGTLISVIIYFRKDILQILVAFFNGIFRGKPFEDPKAHLGWMILLATVPAA
                     VAGALMKDTVESAFNNPRAIALFLLVTAILLFAAEKVPAKDRDLREMNWKDAVWIGIA
                     QAVALLPGISRSGATISGGLFRKFTRRESSRFAFLISIPIMIGAGALSVLDITDWNAF
                     RPLLPALICATLTAAVVGYFAIKWMLNYINKKPFTIFSIYCSVIGIFTFILTFIR"
     gene            59603..62167
                     /locus_tag="PK898_01755"
     CDS             59603..62167
                     /locus_tag="PK898_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOU18789.1"
                     /translation="MKSSEIIRRRRRNRKNKVTRAVKSISALRILSFILICVAITFII
                     LCCAGYVVITQKLPPIENFDDHFASATEPTIIYDRTGTYPLLKLRYPTLEHISLAIDE
                     PDKPCFSSDLVKIIVATHQPNFWSSVGADWHRWSNPDPVTIAERLVQQTFPNQLGSGV
                     EKAILTKILATQITKIYGPQAILTWYLNSAYFGQLAVGADAAARTYLDKSASDLNLSE
                     SILLSAILDTPSLNPVDAPGVMRESYLAQIEKLEENGTLTEEEIRSVRSTNFVIFEPP
                     PLEAEIAYSPGLQQALSSAYETIGQEAIERGGYKIRSTIDYDLQTLLECLTGIHHPSS
                     SDVTEQEKSCPYYPFETRTEDQTAEIAKILSQNPISAVVIDIKSGQMIASIDVGPDGS
                     GKRTARKVFSQHEPGSTLTPFVVLSALNRGISLSSMVWDLAEQRYYLPSGYQNPDGRL
                     QGPIRLRTAVDEDIMGPVAEMLREVGTAPVWNQATQFGITGVNARTPESILFNGGSVT
                     VDMLANAYLPFANQGRLVGTIYDDIPRPKTILSITSLEMAEISAQMRSGISLLDPGLA
                     YLMVNLLAEDKQSYRYFNRPAGTKVGRVLNSNDFWMIGFTPEIMTVVWLGGTPNGTGT
                     DQAAILLWQTIMAAAVKNQPITGWSRPETVSQLMVCNPSGKLPTDSCPDTVSEVFLAG
                     NEPTEYDDLYVRIPINRNNLKMATAYTKFADIEQKVYMNLPKEANDWAIDNHIETMPA
                     EYDPIPEMDASTVKIYQPINFSIFKKTESRTDPLEIKAEINIPEEILSVQVVLGKGFY
                     PDSWYELNNSSEINNGRWTLAKSDWYALDPALYDLRVSVITKDLKYYRADQYFSVQ"
BASE COUNT        16622 a        15060 c        13583 g        16921 t
ORIGIN      
        1 aattttctta aacaacatcc acgccccaac atccggggca tgtatgttgt ttttttcgct
       61 atttgccaga aatctgaaca cagcgaagtt ctgtcgtgac taaatacaat attatggcta
      121 taccatctac agtctgacaa agcagctcga agatttcacc agcggaattc cgttttaaaa
      181 catctaccgg ctggaagaac cagatataca gcaaaaaaat cggcgtacat gtctccagag
      241 cgctgcgaac aaattttaaa cagtttacgg ttatcgccgg tgtattccct attcgcattc
      301 cgatcaaatt acatccgaaa aaagacaagg caaaaagttt gggatcgtta ttgagatgaa
      361 tctataggtc tattaaaagg aacgatgata ttcaccgaac tggaaaagag atgtcagtca
      421 acaaacggat gttataaggg tatgaatacc gcatttaccc ctgtttccat tgcaatacaa
      481 aacaagtcca atgcgataaa atcgatccgg tcttgatcaa aaaagtcaaa aatgaaatcc
      541 tgagtgaaga caatctgaaa agatgatcga tgcatacttg gaatacagcc caaagacaag
      601 ccggattatt cggctcagtc gggagaagtc gagcgaaaga ttgaaaaagt cattaatgcc
      661 atcgctgata tggggaatac cgatccgctg cgcaagaagc tgctagaatt acaaactcaa
      721 caggctgtca ttcgggatgc gatgatcatg aaaaaagaaa cattgaaatt agaaaccaac
      781 ctattcgaag atatttataa ccactcaaag aagaatgtca gtgccgctta tcataataaa
      841 agcgactttg cgatccttca tcgcccgaat cacaccaaca agcgaaaatt ccgccgtaat
      901 acaatactcc cttcccggta tcagttggga agggagcaag gcgcccccgg agggactcga
      961 acccccaacc atttgattcg aagtcaaccg ctctatccat tgagccacga gggcttgctg
     1021 taaatcattc ttttcagcca aaaaagtata ccacgtttcg acatcatttg cgaatcaaaa
     1081 gcccgattgt ttcagaaatt caatcaaatc ctgtttctca attgtttcct gtgtgcgatt
     1141 ggtcagatct ttgacggtga cttttcccgc tgagatttca tccggaccgg caatcaaagc
     1201 gaaccgggca ccgatccggt tggcgaattt caattgttta tccagtttgg ctgcttcggg
     1261 ataggttatc acattgatcc cgcttctgcg aatttcttcg gccaacccgt aagtttccgg
     1321 aagcgtttca ctattaaaac aggtaaccac aatgcgagcc ggttgcgtag ataattccgg
     1381 gtatttcccg tatttttcga gaacgaccga aatcatcaca tcgcccatgg caaagccaac
     1441 gccggagagc gggtcaccgc cgacatccgc caccaaattt tcataccgtc cgccgcctga
     1501 taaagcccgc ccttccggcg ctttatccct ggcttcaaac accgttcccg tataatacag
     1561 caatccgcgg atgacatggg gatcataatt gatataatcc tcatatccca tcgcttggat
     1621 cagcttaaaa atcgtcgaaa gttcctcgga ttctttccat agatcgcgat tttcaaggag
     1681 aacggtcagc gcgttcaact gaaccggcga gacaccacat tccgatgcat attcccgcca
     1741 ggcatccggt ctcaatttgt cacagcgatc aatcaattta taaacagccg gcttcagatc
     1801 ctgatcgatt ccgatttttt ccaattgggc atccatcaga cggcggttat ttacgttaat
     1861 ttgaacctgt tcggatgtca gtcccagttg ttcgaaaaaa gcggcacaaa tagccaataa
     1921 ttcggcatcc gcctcatatc ccgccgctcc gatcaaatcg atattccatt gaaagaattc
     1981 acgggtccga cccttttgag gctgttcgta acgccaaaac ggaccgaatg accaccaacg
     2041 cagcgggtaa accagttctt tttgtttttg tgcgaccatc cgtgccagag tcggggttaa
     2101 ttcgggacgg agcgcaattt caccgccgcc gcgatcattg aaaacatagg cttgttcttt
     2161 caccaactct tccccggatt ttgccgcata cagctccaac ttttccagca tcgggccttc
     2221 atattcctga tagccgaatc gttcggagac aattcgaatc tgatgatata accatgtccg
     2281 aaaagccata tcttcggggt aaaaatcgcg tgtccccttt actttttgaa caatttcctt
     2341 catcattttc ctccctgttt acccgagaaa gtctttaacc atggcctttc cccctcggaa
     2401 tcctctgcat ttcattttta ccggtctaat tgtactcttg attgcagtat aatttgcttc
     2461 atgcatgagc tgtcagcgac ccaaaatatc cttcgtattg tgatggatca cgctgaacga
     2521 aaccatgccg atagtgtcag cgcgattaat ctggtcgtgg gagatttcgc aaacattgtt
     2581 ccggattgtg tcggttatta ttggaagatc atcacggaag gaacaatcta tgccggtgcc
     2641 gtcctccatt ttgatcgtcg tccggcaatt ttcgaatgcg atgattgcgg cagtcaattt
     2701 gaaatcactc gagaaatgac agcctgcccg cagtgcggca gcgatcatgt tacatttatc
     2761 tccggtgacg aattattggt ggaaagtatt gaaattgtca gaaacgagaa ggaaaaatga
     2821 cttcgattaa agttcccatt attgaaaata tcctcgaagc gaacgatttt gtggcggaag
     2881 aaaatcgaaa aattttctcc gaacataaca ttttttgtat caatattatg gctgcgccgg
     2941 gagcgggcaa gacggctttg attgtgaaaa caatcgaatt attaaaggat aaaatccgga
     3001 tcggtgtaat cgaaggagat acagcctccg ttaccatcga ttccgatcag gtggtaatgg
     3061 ccggtgcacc ggctgttcag gtaaccaccg ccggtgaatg tcatatggac gccatgatgg
     3121 tgcgaaaagg gatggaacag cttcctttgg atgatctcga tttggtcatt attgagaata
     3181 tcgggaattt aatttgtccg gctgaatggg acctcggttc tcatgccgat gcgctggtag
     3241 ccagtgtggc ggagggcgat gataagccgt ggaaatatcc gaatatttat cgcaatttgg
     3301 atgttttact gatcaccaag acggatttaa tcccttatgt cgattacgat atcgatcatt
     3361 ttaagagcgg cgtcgagatt ctcaacccgg gactggttac ttttccgact tcggcgaaaa
     3421 ccggagaagg attcccggct tggacggagt ggctcttaaa gaaaacaaaa gcggctgccg
     3481 aaaagtaatt tctcatcaac ttcaaagaca catattctat gaaatcgtcg gaaagtcttc
     3541 attgggaagg ccgccattat caagtgagcg gcatcgttca aggcgtcggg tttcgccctt
     3601 atgtttataa attggctaca aaattcggtt taactggcga cgttcggaat accaccgaag
     3661 gggtggaaat caacctgtat ggcaccgacg gacagcagac cgaattcgaa accgttctgc
     3721 gcacttctcc accggcattg tccaaagtgg accggatcac aatttgtgag attcctttcg
     3781 aagaaaaaaa gacctttaaa atcatcgaaa gcgcccggaa agacggggaa tatctgccca
     3841 ttccgccgga tattgccatc tgcgacgatt gccggagaga aattctggat ccggcgaaca
     3901 aacgttatca ctatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgcga
     4021 cgattgccgg agagaaattc tggatccggc gaacaaacgt tatcactatg cttttaataa
     4081 ctgcattaat tgcgggccgc gctttaccat catcaaagac ttgccttatg atcgaataaa
     4141 taccgctatg agcggttttc ggatgtgtcc ggactgccaa agcgaatatg aggacccgtc
     4201 aaaccgtcgt taccatgctc agccgaccgg ttgtccggtc tgcggaccgg aattggaatt
     4261 caggcagcaa agcgaacccg ctgtccgggg ggaaagagct ttgcaagccg cagccgcagc
     4321 gatccgagcc ggtaagatcg tcgcgatcaa aggtttgggg ggatatcact tggcatgcga
     4381 tgcgttgaac cgagacgcga ttcgcgagct gcgcaagcgg aagctgcgca gcgacaaacc
     4441 gttcgcattg atggcgtccg atctttccgt gatccggaaa tacgccgttt gcggcgatgc
     4501 cgaacaagcc gcgctgcgca gtgaacgcgc tccgattgta cttttggatc ggttacccga
     4561 ttctccgatt ccgcccgaag tcgcgcccaa acaatcgacg ttgggattca tgctgcctta
     4621 cacccctttg catgtgttgc tctttgatgc gatagctccg gatacggaac ttctgatcat
     4681 gaccagtgca aatatctccg aagaaccgat catctgcgat gaaacggatg aggatatgcg
     4741 caagctgttt gcattaagcg actgtatttt gactcacaat cggccgatta tcacccgaat
     4801 cgatgattcc gtcattcgat ttttaccgga ggaagatcgc ccgattctga atcggcgcgc
     4861 tcgcggctat gctccggatc cgatccgact ggctgaggct ttcccgcaaa tcttgggtgt
     4921 gggtggagac ctgaaaaata ctttttgtct cacccgtgac cgttacgcct tcctcagtca
     4981 gcatatcggc gatatggaaa atgtcgaaac atcggcttcg tttgaacaaa acctggatca
     5041 tttccggcat cttttccgaa tccgtccgga ggcaatcgcc tgcgatttac acccgaatta
     5101 ctttaccgtt cgttttgcgg aacgctatgc ggaaaaagaa cggataccgc ttcttcgcat
     5161 tcaacatcac tatgcgcata tagcctcggt tttgggtgaa tatgggcgtt tctccgccga
     5221 accggcaatc ggcttggctt tcgacggaac cgggtacggg agtgatgcga cgatttgggg
     5281 cggagaagtg ttgctttgta acgcccgcgg tttcgagcgc ctgtttcatt tgcaatcttt
     5341 tccgttaccg ggcggggatg cagccgtgcg tcatccggca cgtcaggcat tggcgttgct
     5401 gcaccggttg gaaattccgt ggacggatga ccttccgccg gtcaaagcgc taaaagaaga
     5461 atcggtgaat gccctcggcg tgatcgatca ccaaatccgg caacgcatca actgtacgga
     5521 gacttccgcc atgggcaggc tttttgacgc cgtttccgct ttaatcggcg tatgccagaa
     5581 aattaattac gaagggcaag ccgcgatcga acttgaatcg atcgcatcct cgctgccgga
     5641 ttccgggtat gaattcccgc cggaaggaga tgaaatcaaa ctcaaaccgc tcatcgaatc
     5701 cgttttatgg gatttcaggc acgggatcag ccaagaaatg atttctgcaa aatttcatcg
     5761 cggactgatc gatttaacgg ttgaaatctg cattaaaatc agaaagaaga tcggcggtaa
     5821 tccgccggtt gcggtctccg gcggtgtctg gcaaaatcgg attcttcttg aaggggttcg
     5881 aaacaggctg accgccgaag gatttgaatt attaataccg catcaggtac cgctcaacga
     5941 cggctgtatc gcgttcggac aggtaatgat cgccaaagcg atcctcgggg agaaataatt
     6001 atgtgtctcg gaattccggg aaaaattatt aatatttatg atgaagacgg gctcaggatg
     6061 ggcaaagtcg attttaacgg tgtccggcgt gagacctgtc tggtcggtgt gccggatgcc
     6121 aaagaaggtg aatatgtgat cgttcatgcc ggctttgcca tcagccgaat ggatgagcag
     6181 gaagcactcg aaaccattcg agcactggat gaaatcgatg gactcagctg ttgaccttac
     6241 cgcttttcga aatccggaaa gagccgatcg gattctgaaa cgactgaaaa atgagattac
     6301 ccgaccttgg gtgatcatgg aaatgtgcgg cgggcaaacc cacgctttaa tgcaatccgg
     6361 tatcgatcaa ttgctcgcgt cccaaatcga atttatccat ggcccgggct gcccggtctg
     6421 tgttacgtca ctggctttgg tggatgaagc gatcgcattg gccaagcttc cgaatgtgat
     6481 tctgaccacc tacggggata tgctgcgggt tccgggttcc gcagggaatc tgtttgccgc
     6541 cagagcacag ggatgtgata tccgagtcgt ttacgccgca ttggatgcgc ttaaaatcgc
     6601 ccgcgaaaac ccggataagg aagtcgtttt tttcggcatc ggatttgaga cgacggcacc
     6661 cgcaaacggt atcagcattt tgcaggcgta ccgaacagga attcggaatt attccgtctt
     6721 ggtttcacaa gtttgcgttc cccccgcact ggaaatgatc ctttcctcac cgcagaatcg
     6781 ggtccaagga tttctggcgg ccgggcatgt ctgtgcggtg atgggcacat ccgaatacat
     6841 tccgatcagc gaaaaatatc atgttcccat ctgcgtcacc ggattcgaac cgatcgacct
     6901 gttagccgga atcgaaacgg tggttcggga tctggaagcg ggttgttgga gtgtgagcaa
     6961 tgcgtacagt cgcggcgttc cagcggcagg taccgccggt gcattggcga ttattcatga
     7021 attattcgaa ccctgcgatc gggattggcg cggaatcgga ataatccccg acagcggttg
     7081 gaaattaaaa gacaagtatt ctgcttatga cgccgaaaag aaattcggtg ttacggcaac
     7141 ggaaacccat gaatccgacg tctgcatcgc cggggccatt ctgcggggtt tgaaaacccc
     7201 accggaatgt cccgcgtttg ccgcttcttg cacgccggaa caccctttgg gagccgcgat
     7261 ggtctccggc gaggggacct gttccgctta ttatcgttac cggagaaatc atgaccgagc
     7321 ttaacatttc ggaaggattc acctgccccg cccccattca agcatatgat cggattgtgt
     7381 ccgggcatgg cagcggcgga aaaatgaccg ccgatctgat cgaaaagaca ttccggaaat
     7441 atttggaaag cgacggattg gcgcagggga atgacgccgc gaatctgccg atcccccgac
     7501 aggtgaaaag gatcgccgtg acaaccgatt ctcatattgt ttctccgctt tttttccccg
     7561 gcggagatat cggagcgctg tccgtctgcg gtacggttaa tgatttgctg acctcgggtg
     7621 cggtcccgca gtggttaacc gcgggattta ttctggaaga aggcttcccc ctttcggacc
     7681 ttgaaaaaat attggcatcg atgcgggaac gttcggaaga agccggaatt caaatcgtcg
     7741 ccggtgatac caaagtcgtc gaacacggca agggtgacgg aatatttatc aacaccagcg
     7801 gtgtcggttg gatcgttgat gatttccaac cgggaggcga aaaagcgcaa acgggagata
     7861 ccattattct gagcggaacc atcggtgatc acgggatggc ggtgctgtgc gctcgcggtg
     7921 aaatgggttt tgaatccgat attcgttccg atgtcgcccc tttgaatgag atcgtgaatt
     7981 ctttgcgccg agccgctccg catacccatg ttttacggga tccgacccgc ggagggttaa
     8041 gtacaacgtt gaatgaaatt gctcttcaaa gccatgtatc ggttgaaatc gatgagacgg
     8101 ctgttccgat tcgtcccgaa gtaagatcga tttgcgattt attgggattt gatcctctgt
     8161 atgttgccaa tgaaggaaaa ctggtcattt ttgttcccga agtcgaagcg caggatgctc
     8221 ttacggcaat ccgcgcctct cgctacggag aaaatgccgc cgtaatcgga cgtgtgattc
     8281 atggcgatcg gtcgccggta acgcttcgaa ccgctttcgg taccggccga attcttgaat
     8341 ctctgccggg tgaaatgctg ccgagaatct gttgattgga tactttcggc aaaagacgga
     8401 catgcaaaat acggaaaaca accgccccta attttttcgg ggcggttgtt tatccgtaaa
     8461 cttttatctg gatttcggct ttcggggttg ggtatgctca atcgccggcg gctccgtata
     8521 aggcggctgt tgcggcggca tttcatttcc ctgtgggtac ggcgcgtgat aagggggcgg
     8581 aggataagca ccgcgtgtcg gcggctgttg aggttgcgga ggataaggaa aagttcccgg
     8641 accggattga gccggatatc cggagcgact ttcccccggt tgcggatagg aaggataacc
     8701 gctcgattcc ggtcgcgtct gcggataatg agacgttccg ggcaccggcg ggatcggagg
     8761 acgaggaata ttgggcatgg ggggcggtgg aacctgtgaa ggtttcggtt gttgggtttg
     8821 gctgccggtt ttaaccgcag gaatcttttc ggcgggagcg atcggcggtt tattgaattc
     8881 atcgaaacca tcgttaaacg gttccgcagc aggtgaagat gatccggcca attctttctc
     8941 catatccgaa aacggaatgt attcttcttc cggtttttcc gttgtcggtt tttctttttt
     9001 ggctttctta acaggctgat ctttggacag tttttttagc cgttcctgta acgcttcttc
     9061 cgcatcccga tccggaatga tcaattcttc ttcacctttg atttcggata cttcttcgga
     9121 aggaacggat tcgctttctt cggctttttc ctcctgccgc gtatatttgt cttcctgttt
     9181 gggcttcggt ttgacaatca atccggttaa tagcgccaat tcaatttgcg ggtcagcaaa
     9241 aatggacgga acgatatctt ccgtgattcg gcgttcggct tcggtccgat attccggatc
     9301 caatcgataa cgcgcgatca caaaaagctt ccgcagtaat acccgtttga tttcaagcgg
     9361 ggcataggat gccaaatcaa atacgtctcc gctcgtttct tcgtccacta atttcgaaat
     9421 agcggtttta gccatctgat tggcttcttt ttcggaaatc gtattccagt tgcgggtacc
     9481 gataacttca cgaaaagccg gaaggacatc ctcttcttta atttttgcgc tctttgcgtt
     9541 ataaacgata atgatcattc cgatgatacc gagagaaatc gcattgcgat caatttcgtt
     9601 ctcatcgaca atatcatagg aaaagccatc ggattgaatc ggatgattgg gaatatcacc
     9661 tttccacgac caggtatcct gtgaaaaatc aagtgcttcc gggtctaccc caaaattcaa
     9721 tcccgagccg attgaattca tttgtttctt tttccccata tttacctctt aatcagcgga
     9781 tttctcaata tttttcaatc ctgccggttt ttctaattgt aacaaataaa tcttccgctg
     9841 attttcactt ttccgaacaa ctcatccggt aaatatcgat cagcaaaatt taccgaacaa
     9901 tcggatttca gatatcttcc acacaaaacc ggatcgttcc gctgatactt tcaccgggtt
     9961 tcagaatcaa taaatgcgct tcctcttcga agcctttcgc ataaagatta tgacaatcgg
    10021 ttgaattggt ctgattttcg acacacagaa acgattcgaa cggcggcgta taagtgacgc
    10081 tgtgggtgaa aatatccgac gcatataaaa ccatcttttt gccgagcgct tcataaatga
    10141 ttgcctgagg tttcttcggt tccattccgt accacacctc atccaaattc aatccgctga
    10201 gctttgtagg cttgcttaaa tcagccggac tcttatccaa gtcgatcaat ctgcccgtgg
    10261 gaatcaaatt gaccgcttcc atccatttct tcgccggaac atataacttc acctgactct
    10321 tcaaaccgag cacattaaaa taagggtgaa ttccgaaacc gaaaggaaat cttttttctt
    10381 gatccaagtt ggatactttc acctgaatgg tcaatccgtc cgcgcccaaa atataggtga
    10441 gttccagact gtttgcaatc ggaaacagtt tatagatcgg atgtccgggt aaaatttcca
    10501 atttggtttt cacagagata tcatcgtccg tgacgatcgg tgtctcaaat tgaaacggcg
    10561 tatcataaac aagtccgtgc agagaacgac cgccgtcgtt atcgggcaac acatattccg
    10621 ttccgtcaaa agtgaaccgg caattccgaa tgcggttcgg aaacggatat aaaacgggtg
    10681 tgccgaagaa ttttgacccg atcaccggca tcggagcttg atgcagaaac tcaattccgt
    10741 ccaccgagta actgaacaaa ttggatccga attccggtaa taacttggct tccgtcttcc
    10801 gcacatgatc attctccgac caaaggcaga tcattttttg atcgaatacc ggatgcttcg
    10861 caaaattgta accgtacatt agtattgttc ctttccgatt gtatgacccc gaaaacactg
    10921 gcaaggtccg aatggataaa aagtatcttt ctgtcgcaaa acgccatttc tcgatggctt
    10981 ccgctgatat aattataatc atggataaag atcggttgat taatccgggc atcatctcat
    11041 ccgatgaccg agtggatttg gcgttgcgtc cgaagcgctt aaatgaaatt atcgggcagg
    11101 atcaaattcg tgaaaatctt tccatattat tgaccgccgc caagcagcgg aatgagcctt
    11161 tggatcatgt tttgttttac ggcccgccgg gtttgggaaa gaccacactg gcgcacgtat
    11221 tggccaatga aatgggtgtc agcattaagg ttaccgccgg tccggcaatc gaacgagccg
    11281 gggatttggc tgccattctt tccaatcttc atgaagggga tattttattc atcgatgaaa
    11341 ttcatcgctt aaatcgcgcc atcgaagaaa ttctttatcc ggcgatggaa gattcggttt
    11401 tggatatcat ggtcggcaaa ggaccttccg cccgttcgat tcgtttaaaa ctcccgcgtt
    11461 ttaccgttat cggtgcgacc acccgtcttt cgctggtcag ttcaccgctg cgggctcgtt
    11521 tcggcgctgt ttaccgtttg gattattacg atcagccggc gatggaaaag atcatcgaga
    11581 atgcggctat taagatgaat gtgaccgttg acgacggcgg tatcaaagag attgccgtcc
    11641 gttcgcgcgg aactcccaga atcggcttgc gtctccttcg acgagtccgg gactatgcgc
    11701 aagtgcgcgc cgacggcacc atcaacgagc aagtttcacg ggacgctttg gaactgatga
    11761 atattgacgt cgccggtctg gatgatgttg accggcgtgt gctgttgaca attattgaaa
    11821 aatacaacgg cggaccggtc ggcctcagta cgatcgctgc ctcgatcagc gaagaaacag
    11881 acacagtgat ggaagtggtc gaaccttacc tcttacaact ggggttcatc gatcggacgc
    11941 cccaaggcag gaatgctacg cgaaaagcgt acgaacattt ggggatccct tataatgatc
    12001 agaacgcccc caaacaagga tctttatttg aataacatac ggaatttaat agcgagaatc
    12061 ttgaccggtc gatttacttt ttccgttata ccctcacaga aacaataaaa caaaatatat
    12121 gcagacatcg gattttgatt actatcttcc tcctgaatta attgcgcaaa cacctttgga
    12181 accgcgtgat tcttcccgtc tcttggttta caaccgaaaa acagatgaaa ttactcattg
    12241 ccatttttat gatatcggaa atttcctcga gccgggagat ttattgatcc ggaatcaaac
    12301 ccgggttttg cccgccagaa tctatgccaa gaaacatgaa accggcggaa aagctgaaat
    12361 attactcttg agacggatcg aacccgacac atgggaagct ttggtaggcg ggaaaagact
    12421 tcatgaggga acgattcttg atttggagga aggactgagc gccgaaatcg ttcaagacct
    12481 ggataattcc cgtcgtttga tacgttttac cggtcctttc gaggatttta tgcaaaaaaa
    12541 cggacagatg ccgttgccgc cctatattca taagcgtctg aaggatccgg agagatatca
    12601 aacggtatat tcccgacaag atctggccgg ctcggctgcc gctccgactg ccgggttaca
    12661 ttttacggaa gagctgatgc aaaaattgga agctcgccaa attcattttg cggatgtcac
    12721 gctgcatgtc ggtttagaca ctttccagcc cgtcagcgaa gcagatccgc aaacccaccg
    12781 gattcataag gaatggtgtg agctgtcagc cgataccgca aaaaaaatca cggaaacccg
    12841 agcgaacggt catcgcatag ttgcggtcgg gacgacaagt gcccggactc tggaaagcgc
    12901 ggcacaagcc gcactgaaag cgggatcgcc ggactgtgtc attccgatca gcggagatac
    12961 cggtatctat attttgccgg ggtatgaatt tcgggctgtg gatgcaatga ttacgaattt
    13021 tcatctgccg aaatcaacac tgataatgat gatcagtgca tttacaagcc gtgaaaaaat
    13081 attatctatc tatcagcagg caatcgatat gaaatatcga tttttttctt tcggcgacgc
    13141 tatgctgatt ttataaaaaa gttcaactgt tttctgaaaa aatcttcctt tgatgcggat
    13201 caaatttcga ccggctcttt acttgaccgg acaacttgaa ctcggttttt caacggcact
    13261 tcggtaaagc acatcctgat attcggactt tttttcgaat tctcgttgtg cgaaaggaca
    13321 gaccggtaat attttcatac ccatttctct ggcgttttga accaccaatt tcaccaattc
    13381 ggaagcgata ttttgtccgc ggaattccgg atcgacaaat gtatgatcga tcgtccaata
    13441 atcctgacag atggaaatcg tgcaacttcc gatttgtttt ccgtcatcgt tgagtgcttt
    13501 agccttgcgt ttttccaatt cgaatttgta cgaaatcatt atcgtgtttc tcttactttc
    13561 tggtttgata ttcaccttcg atcacccatt tataggtggt taattcacgc aacgccatcg
    13621 gtccgcgagc gtgcaaaggt tgagtggaaa ttgcgatttc ggcgcccaat cccaattcgg
    13681 ctccatcggt aaaccgtgtg ctggcattga cataaacgca agccgaattg acttctgtcg
    13741 taaagcgacg gatattttct tgattttccg ataagattcc gtccgaatgt ccggtaccgt
    13801 gtaacgatat atgatcgatt gcctgtttga catcatcgac cactttcaca cttaaaacca
    13861 gcgataacca ttccgtatca aaatcatttt caccggcttt ttcgaagtga atcgatttgt
    13921 caccgttcag aagcaaaatg ttataagcat tgccgtcgac tttaaacgat acgccgtctt
    13981 ccgcaagttt agcacacaat ttcgggataa attcttccgc aatcgaacga tgaatcaaaa
    14041 ccgtttctaa cgcattgcaa accgtcggtc gttgaacttt tgcgttgcgg ataacctccg
    14101 acgatttctg tagatccgcg gattcgtcca caaacaggtg acaaataccg atgccgcccg
    14161 taattaccgg aattcgactg ttttttcgac aaaattcatg caatccggac ccgccgcgcg
    14221 gaatcaccat atccacatat ggatccgatt gtagtaattc caacatgaaa gaacgatcgg
    14281 gggaatcgat gaattggatt gcatcggcag gcaaaccgtt agaggacaag gcattttgaa
    14341 tgaatgaaat cagcacttga ttggttcgaa tcgtttcttt accgcctctc agaatcgccg
    14401 cattccccga tttaatcatc aatccggcgg agtcgacggt tacattgggt cgcgattcat
    14461 aaatcacggc aatgacaccg atcggaatcc gttctttccg aacttttaat ccgtcgggat
    14521 tggtatatcg ctcgaagatt tcacccaccg ggtccggcag ctcggcaaca ttccgaagat
    14581 cttttgccat ccctttgatc cgttgttcat tcaacagcaa tcgatcaacc atcgccggag
    14641 ataaaacccc ttccgcctct gcgacatctt cggcatttgc cgcaagaatg tccgtatgat
    14701 cggattccaa caaatccgcc agacttaaca aaaaagcgtt ctttcgttcg gtctctgccg
    14761 tcgcaagaat tttgctcgct tttcttgcat cctgacccat ttgaagaatt gttttcatcg
    14821 tgttttcccc ttgatcatat tatcctgatg aacgacttcg tcagcatagg tataccccag
    14881 aatcgtttcg atttcacttg cttttgcacc gcaaattttt tgaatttcgg aagccgagta
    14941 attgaccaat ccgacggcga tttcctctcc gtcgttattg accaaccgta ccgtatctcc
    15001 tcggtcaaat tcaccttgga cgccgataat tcccgccgga agcaagcttt tcccgcgttt
    15061 gatagcctgg gtggcgcccg aatctaaaat aatcgcggct tcattcgaac ggaatccggt
    15121 caacaaatat cgtttacggc tttcgagaac cgtgttggtc gctcgaaaca ccgtgccaaa
    15181 ccgttctccc gctgcgattc gcagcagcac atccggaatc gttccgttag cgatcataac
    15241 cggagttccc atcctgcggg caacgtctgc cgcccgaact ttcgtcagca tcccgccggt
    15301 ccccataccg gtcaaactgc cgccggcggc ttcccaaacg aaagagggaa tttcgacacc
    15361 atccacgaga tcgatgagct ttgcttccgc atgaagttcc ggattcatgt caaaaagtcc
    15421 gtcctgatcc gtcaacaaaa ccaataaatc ggctccgatc agccccgaaa cgtaggcgga
    15481 aagcatatcg ttatcgccga cgcggatttc atccgtcgag accgtatcgt tctcgttaat
    15541 aatcggaatt acatgctgcc ccaataatcc ttcgattgta tttcgggcgt ttaaataact
    15601 tttccgttga atgaaatcat tccgtgtcaa cagaatttgc gccgccgcca gattataaat
    15661 cttaaacaaa tccatataaa tggacattaa atagggttgg ccgacagagg ctaaaatctg
    15721 tttcccggga atatgcttcg acagatgagg atagttcagc aattcacggc cggctgccac
    15781 cgctccggag gataccaatg taaccatcgt tccgtgacct gcgataacac tgagctgccg
    15841 cgccagatca accaagcgcg gtttatccaa atggttcgat ccgcccgtta atgttgatgt
    15901 tccgactttt ataacaattc gttcgtaaga cattgaatat caccaaaaac caaatgacag
    15961 gtaacaggaa aaacggtaat cgattggttt attccctgat tgtatccgat catttcgata
    16021 tttcctttag caataaaaac aatcgttggg gatttcctga aaaaaagagg ctgacaatcg
    16081 ttcatcagcc tcaagcttac tatttctttg aattactttt tatccgactt tgttattcag
    16141 agtgcggttc agaatttcat cctgctcttt ggatacaccg gatttctttt tattttcgct
    16201 tttatttcgg gctttggctt tttccatcgc ttctcgaatt gcgatttcca ttgcggtcgg
    16261 ttccgcttca ccgctcggat tttcatcggt aaagctcatg gtaattcctt cgctgtttaa
    16321 ttcggcccgt ttcttagatg ttttcttctg ctgccgcggt ctaactttct cttccgaggg
    16381 agcggaaacg gcattcgtgg tcctggcttc ttttaactgt tgcgcacgtg cgctgaccgg
    16441 acgggctgtt ttctccgccg gcgcctcttc gacctccggt tcgggttgca aagctttaat
    16501 gctgagtttg atctgcttct ttcgccggtt gacttccaaa accttggctt ctacttcatc
    16561 tccgaccttc aagatatccg aaggttggcg aacatagcta tgcgcaagtt cactgatatg
    16621 aatcaatccc ggtcgctccg caccgatttc tacaaaaaca ccgaatttct ccatccggac
    16681 aacggttccc ttgactacca tgtctttttt aatctcacgc cactcgagat catacggttt
    16741 gatcatggtc agttcaatcc ggccgtcttt tactttcttt acccaaacat ccacttcctg
    16801 cccgacggtc agtaaatcat ggatattatt ggtaaattcc gccccttccg gacgggcaat
    16861 ctgggttata tggattgcag cgggaatatc taatccgata tcgaccaaag cgcctgccag
    16921 gctcagtttc gtaaccttcc ctttaaaatg atccttaatt ttcggaccgt ttgcgcctgc
    16981 ggactctcct ttcgagtcga tatcagtcac agtatctcct ggttgatccg ctgccgttac
    17041 cacggcaaca tcatctgcaa cgttttccac tgcgctctgt acctctccga ttttttcgtt
    17101 tactgcattt ttttcttcag gtgatacaga ttcttccatt ttttcccaat ctccagatat
    17161 tattattcgc aacgtttgat tatactcgta taatttaata cttgtcaatc aaaaaaacaa
    17221 ttttcgtgca aaattcatta tattcccccg aattcggcaa aaatcgattt ttataaaccg
    17281 gtttcaccgt tttcttccgt atttttctgg ggtacagacg gctcttccgg gatttttccc
    17341 gttaataaaa tttgcgcagc cgcatccagt tgcgggtcct ttccggcttc cacatccgca
    17401 tcgctcattt ccacgactac atcaggagtg ataccgacct tatggattaa ctttccggcg
    17461 ggtgtcagcc aatgagcgat cgtgatactg accgcgccgc cgtttgaaag ctcgggttga
    17521 atctgcatag atcccttccc aaacgtctga actccgacaa atgtcgcccg atgataatat
    17581 tgcaaagcac cgattaagat ttccgaagcg gacgccgtcc cttcgtttcc caaaacaaca
    17641 atcggcaaat ccggatcgac aacgggatct tcctttgtat gatattcggt tcgggatccg
    17701 tctccggcct cttcgatcaa aaccaacgaa tctttgggca agaatttcga agccaccgat
    17761 acgcaggtat ctacatatcc cccgccgttg ttgcgcaggt ccaaaatcaa ccccttggga
    17821 ttctgcggca gcagctcatc caatgcctgt tgcaattctt ccacagtcaa atcaccgaat
    17881 tgcgttaact gaatataggc tatcccgtca tcccgcattt ccgaagccac catcggtgta
    17941 acgaccgcat cgcgaacaat cgatacatca aagggctctt gatcgtcccg tttaatggtc
    18001 aatacgactg tcgatccttt cggacctaag atcttgttta acgcgtcttt gggatccact
    18061 ccggtcatat cgacgccgtc gatggcaatc accaagtcat gcggttttaa tccggctttc
    18121 tccgccggac tacctttcat cggagaagtg atttcgacat aatcaccgga cacatcaacc
    18181 catgcgccga tcccttcata ttcttcgccg gcaatatatt gcgattcctg tttatagctt
    18241 tcgggatcgg aatagcgggt atgcggatcg cccaaagatt ccagcattcc gcggatcgcg
    18301 ccctccatca ggctgttatc atcgaccggt tgatcgacat aatactgatg aaccaaatcc
    18361 caagtttccc aaaacggctt aaataattca tccgtgtttt gttggcgttt catccccgta
    18421 ccttgctccg tgaccgacaa taatttcgga agcaaggacg attcggatga aatattgatt
    18481 ttgccgacag tttccggtaa catttttatg atatatccgg cgctgaaacc ggatacaaaa
    18541 caactgatta aaataatgac aatcgcaatg attcgaatga ttcgttgatt catttttggt
    18601 ccttatctct ttccagattc tctaatctgt ccatttcctg acgcaaccgt ttgtaatgtt
    18661 cggattccgg aaacaggaaa tcaccttttt ttacttcttt aaccttcttt gtcagttttg
    18721 aaaaggaatc atcttgattg cttttggagg ccggtttccg tcttttttca aaataagctt
    18781 tgatcttttc acggtagaca attaacaata tgttaatccc catgacaacg acaattaagg
    18841 caattaaaat gatccaacct tttgtagtca tgttattatt tccgaataga tttccaattg
    18901 aggaagatcc tctattctcg aaagaaacgc atcggcttct tccgatcttg ctcgcgatcc
    18961 gacttgaatc gtgtaaaaac ccagttgatg agccgtgctg atattggtcg gattatcatc
    19021 cagaaagata catccccggc cgctgtcgat tccggtcatg gcgagcgctt tctcaaaggc
    19081 ttcctgttcc ggtttacaat acggataaac atccaggaaa tcaataattc cgtcaaaaca
    19141 ttgataatta tccaagagtt tcaatacacg aatcgcatga aaacgatcgg aattggtaaa
    19201 aataaacttc ggaatttgaa ttttttcaat cagtgcccga acttcgggag ccggctgaat
    19261 atacggcgaa atatcgacat tatgcacata atgcataaaa tcatacaaat ccacttcgta
    19321 gttttttacc aaccctttgg cggtcgttcc ataagtttta tacaggtctt cgcgaaaatt
    19381 cttcacttcg ctgtcgggga ttgccaaaga acgccggata tattcattca tccgatccgt
    19441 aatcacttta attgattcat tccccatcgg gtacagagtt tcatctaaat caaaaaaaac
    19501 agctttgtaa ttcatgcaaa aaagtataac cgaatcggag atcgattaaa tggaatatat
    19561 cggaaattct gacgaaataa accgctattt cggcaggaca atcggagtgg aaataccgtc
    19621 atgacgaaag acaatcatcg gaacatgata gacagcggtc aggttgtttt cggttaaaac
    19681 ttcctccggc gttcccattg ccaagatatt tccctgtttc aaaataatga tttcatccgc
    19741 aaatctggca gccaaattga ggtcatgtaa aacgatcaac acggcggttc ccttttcgcg
    19801 gctcaaattt cgaaccaatg aaagaatccc gatcgtgtgg ttcaagtcca ggtgtgtggt
    19861 cggttcatcc aaaatcatca ccggcgtatc ctgtgccaac gctctggcta aaattacccg
    19921 ttgttgttct ccgccggaaa gagaacgaac atcttttttc cgaaggtcaa aaatggcggt
    19981 ttcacgaatc gcctcatcca cccgccgtcg atcctcttcg gataatttcc ccaataaacc
    20041 gagatacggc gtccttccca tcgctacggt ctcgtaaacc gaaaatccat cgggcagcaa
    20101 ggaattctgc ggaacaaccg acaggtattt ggcacgttcc gtttcactca aatcagccga
    20161 gttttttccg tcatacttga tttggccgcc ggaaagttta acactgccgg taataccgcg
    20221 aataatcgtc gttttccccg aaccgttcgg accgattaat accataactt tcccttgccg
    20281 aatatcgaac gaaatatttt ggacgaccgg ttgatcgccg taatgaacgg atacatcgga
    20341 aagagagatc atcgttcgat ttccctcccg gtatttcgat ttaacagcca aaggaaaaag
    20401 ggcgcaccgg ctatggcggt gataatccct accggtaatt cctgcggtgc aataatcacc
    20461 cgcgcaatca catcagccag taacaggaaa aaggcaccgc ccaatgccga taaaggaatc
    20521 aatttccgat aatctccgcc cgtcaaaata cggatgacat gaggaatgat taagccgacg
    20581 aatccgatta ctcccgaaaa agagattgcg gccgccgtgg tcagagtcgc gaaaaaaaca
    20641 atcccgattc gggttttatg aaccgagatt cccagatttg cggcttgttc ttcaccgaat
    20701 tgaaggacat tcaaacgatg tgacaataaa aactgaccga ttattcctat cagaataaaa
    20761 ggaatcacaa tcttaatcgg ttcccaaccg ttaacggtca ttcctcccaa catccatgaa
    20821 aacgatgttc gcaattctcg gtgagaaaac aacatccaaa cggaagttac ggcggtgata
    20881 aaagaattta cggccacgcc ggaaagaatc aatccggtat tgccggttat tccgctgacc
    20941 ttgcccaatt taaagacgac ccataccgtc agcaacgata ccacaaaggc taaaatcggc
    21001 gtcatcatgg tcccccattt cgtataaggc cactgtacag acatagcaat cacagccccc
    21061 aaactggcac cggttgagac gccgatcaaa tagggatcgg ccagcggatt ccgaaataat
    21121 ccctgatagg aagcgccgct gcaagccagt gcggcaccgg ttaacagcat cataatcgtt
    21181 cgcggaattc gtaagacaaa taaaatccgg tccgtcccgg caagttcggc aggcaaatcg
    21241 cctccggtta tccgatattt caatatatcc catacctgag aaatgccgag cgggacacta
    21301 ccgacccata taccggcaat actcataacg aatattccga tgagcaaaaa aatgcctaac
    21361 cgggatgccg atctgcgcat agaacaggtg cctctgctta tttcttttcg aacagttccg
    21421 gatgaatata tccggctaaa atttccagag catcgaccag acgcggactc gggcgtgatc
    21481 cgatatcgga attaatgggg tagatctttt tatttttcac tgcggatagg acatcccagc
    21541 ccggtcgttc gatgacgctt tccggagtta tcccgtagtt gctgtcaccc agaataatga
    21601 tctccggatt ggatcgaatc aacgcttcca aactaatctg aacccattct cctttgagat
    21661 cggctgcgac attctttccg ccggcccgcg taatcagctc atcaatgaag gtaccgctgc
    21721 tcgccgtcca gggtttggaa acgtcggtcg catccagttc ataaaacacg agcggatgtg
    21781 tggttgcccc ggctatcgtt tcatcgacga cggcaatcct gtttttcatc tctgcgatca
    21841 gttcttccgc ctgctcctca tgaccggtta cttctccgac tttcataaaa taggaatcta
    21901 agtcatccag tgtcttggga tttttaagat aatagacggt caagcccata tcttccaatt
    21961 gttttacctg atcaaccgcc gtcagttccg aaatgatcac caattcggga tcgagtgaaa
    22021 gaataagctc tttgttaatg gacatatcat aatttgtgac gatcgctttc ttcgtcactt
    22081 catccggata attggaagca acatcgactc cgatcaattc atcacataaa tccaatgcgc
    22141 atacgatttc ggttaccgac ggcgccaaag aaacgacgga ttccaaggga agttcaagcg
    22201 taattttccg atcaaggtca tccgtcaccg tgattttttc agcgacatcg gtatcgcgga
    22261 cggccgaaac cggcatggca attgaaaaaa gtatcgtaag aaaaacaaca caatagaata
    22321 aagtcttttt ccgcataatt cacctcgtta tatagatttt ctcgttccgg aacttggcga
    22381 taaaaacgaa ccattccggg gccaaccgga gagcgggaag ttcgttatga ggatattcaa
    22441 aaaagaaaac ccctgacggg gtctaatcgg catgccttcc caatccccga ggaacgattt
    22501 ttgtcgaaaa caaaggtctg gcttggaaga tgatcttctt acagttgcgg gtcagcatcg
    22561 gtttcgaacc gatttccttg ttttccactg gcttttgaat tataccaaat ttattcttcg
    22621 attctttatg gttatttgcc gcgtcgtgaa ggatatccct tccttgtccg ttccggtaaa
    22681 atggagactt gcggattacg actgtacatg ggaacctgaa acatttgcgc gatctgacaa
    22741 acctgaaagt caaccaaacg actgcgaata ttggggtcaa tatcttggat gggtaaaacg
    22801 gtggtaatca cggtgggcag cggcgttaaa taacgatggt ctaaaatttg gaacagcttt
    22861 tctttcgccc acggggtagc atttgccgta tcgagatgat ccaaaatcaa aagcggcgca
    22921 ttccgaattt tttcgaaagc gatgtcatag gaagtggtac tgaccggact gaatgtcgcc
    22981 cgcagatgat ccagcaaatc agccaccacc acaaacaggg gatgatccat ctgatgctgc
    23041 cggtaatttc cgatcgcagc cgccaaatga gtcttaccga cgccgttcgg gccggaaaat
    23101 accagccaac cggtgggatg ctgcgcaaag ttatatgcca aattgaaggc gttggtcaac
    23161 tgagcggagg cttcttcgga cagattttct ccgactcgcg aatcaaaatt ttcaaacgtc
    23221 cggttggata aacgatgaag tgccgacaaa tcgtccgatt ccaccgtatt tgccatcggt
    23281 tttcgataat cgggcgcttg aatcacgatc cgggtaacca aagacggatc ttccagccgc
    23341 gaagcaatcc gcccgtctaa gtcctgcaag cggcaattgg tggtgataac cgtcggcagc
    23401 cgatttacgt aacgataatt caaaatctga taaagttttt cctctgccca gggagttgcg
    23461 ctttgggtac ccaaatcatc taaaatcaac aaaggtatat tgcgaatttg atcaaaacgt
    23521 tcggcataag taccgctgct gtcactgaac gaggcacgaa tccaatccaa caggtcggga
    23581 accggtaaaa aaatattggg aatatgatcg gctaatgctg cattggaaat cgctgccgcc
    23641 aaatgagttt tccccgttcc gtaattaccg gtcagcagca gccacccgtt tctttccttc
    23701 gcgaattttt gagcatgatc gaatgctaat tttaaacaca accgttcatc cggccgcagc
    23761 tgcccgcgtc cttcgacatt gaaagtttca aaactcatgt cggcataacc ggaaagatta
    23821 ctctcgcgga ttaattgatt ccgggtctct tcttccagtt tggcttttcg gcaagaacaa
    23881 acatccatct tcccgaaccg gggatcatca atcggataat cgccggtgac aaaaccgatc
    23941 ccatggcaaa tcggacaatt cggatcgcct aaataagctt cgcgattcat atcatccaag
    24001 cttttcggat tcttcggggt gacattaaag tcttttttta cccgttcaat aatgtcatca
    24061 atagattcca ttccgtccgt tttccttcca tgatttcaaa atcgtttcga tatatcgcca
    24121 gctccgggca ttgttcacca ctgcgatatc gatggcttcc gtaatccatt cttccgaata
    24181 catttgttcc gcgtctttta aacgatccgc cattaaggga gtgagcggac cgatgttttc
    24241 ctcatataac tggaaaatgt tcgggcgaaa cagattcaac gttcccgaaa gatgcagaaa
    24301 agcatccggt ttccattttc ctttttctaa attttccacc gccatccttc cctttgggct
    24361 gttgagaaaa tagagagatt ctccgccgta ttccactttc aacaaagtgc cgcgtaacac
    24421 tgccttttcg agaccgtcct tcaaagcgtc ttcgcgatct tcttcaattc cggctaatcc
    24481 atccataaat tcggcggcat cagtaaaatc cgaatagagt atatagcgct ggtcgccttc
    24541 gaattgatta agaagatgaa tcgcaaacaa actgaccttc aattcggtca tcgaatcgat
    24601 ttcaggcagt atttcctcaa agaattgagt cgggataacc tcgattcccg tattaaaacc
    24661 ggaaaaactc atcgaattcc tccgctgtcc gaaatgcggg ctgcattttc aaagcgggcc
    24721 agctttttaa caaagaccag gtcgatatct cccaccggtc cgtttcgatg tttggcgaca
    24781 accaatttca tcgctccgtc taaaccgact gttttactgt catcggtatt ggggttatga
    24841 agaaacatca ccacatcggc atcttgttcc aacgaaccgg attcccgcag atccgacaaa
    24901 atcgggattt tttccgaacg ttgttcgaca cttctcgaaa gctgggcggc tgtcaaaacc
    24961 ggcacattta attccctcgc caaaatttta agatttcgag agatatagga aacttcctga
    25021 acccgattct catttcggga atcggtactc attaattgca gataatcaac gatgaccaaa
    25081 tccaaatgat gctccaaatc caatcttctg catttggatc ggagttgcag cggcgtaatt
    25141 gccggcgtat catccaaaaa aacggtcgta gtccccaaca catccaatgc atcgatgaac
    25201 atcggaagtt catcttcagc caatttcccg ctgcgaatct tttgactgtt aatccccgtt
    25261 tgttgcgcga tcaaacgaat ggctaattgt tcattggaca tttccaatga aaacattgcg
    25321 acgtgcatat gataatttaa tgcggcattt ttcaatacgg acagcagaaa accggtctta
    25381 cccattccgg gacgtccggc aacgatgatc aaatcggatt tttgcatccc ccccaacagg
    25441 cggtccaaat ctttgaatcc ggtcggaact ccgacgatat cctgatcttg ccgcgcgagg
    25501 agccgtactt catcgtaaat tgttcccacc acgctgcgaa tcggttgaac ctcgtttcgg
    25561 gttctttttt cgctgacatt gaaaatcgct ttttcggaat tatccaaaat ggtgtccaaa
    25621 cttttggatt cgtcataggc atattgttgc atctggcttg ccgctgtcaa aatctgacgc
    25681 cgcacacttt tttccgcgat gattttggca taagattcgg cgtgcatcga agagggtgta
    25741 ttgcttgcca gtgaataaag ataaagcttc ccgcccgctt ccgttaattt tccgttcttt
    25801 tccaattcgt tggcgacggt ttggacgtca atatcttgtc ctttttccag aagagcggca
    25861 aacgcttccc aaatccagcg acagcggtga atataaaagt cattggcgga gataatctga
    25921 gcgacatcat aaaacttttc cgaattaatc agaatcgctc ccaataccgc ttcttccgcc
    25981 tcacggctgt gcggttgtaa gcgtagatcg gattcggaaa cttccggagt gaaattctcc
    26041 gtaggataag gtccgtttat ttccatatct tatatcggga aaagaagatc atcggaattt
    26101 gaatccgtgt ccttcttttc gttatcctgt tttcccgaac ggtcgctctt tccggtttca
    26161 ccggaatcgg ggagatcaat atttttcaaa aagtcatcga aaatattcaa tttcgcatca
    26221 tccacaaact cttcttgatc cggctccgcg gcgaaatttt tctttgccga atttaattcc
    26281 cgaatatctt tctcgggttg gattccggct gaaagtaaaa cggttttatc gacaaaaatc
    26341 ggcactttgc aacgaatcgc cagagagatc gcatcggaag ggcgactgtc cacaagtatt
    26401 tcgtttcctt gggattccaa aaccaaaaat gaataaaaag tatcgtcctg aatcttcgaa
    26461 agaacaacgt tcctgagttt cccgtccaac gctgcaataa catttttcag tagatcatgc
    26521 ggttgagggc ggctgacttc aatcccctgc agcgcgatca caatcgattc cgcctcaaac
    26581 gaaccgatcc agatcggaag gtacagttca tttttcggtt catgcaaaat caatacccgt
    26641 tgctgtgtgg ttaaactgac tcgaatacta tcgacggtta cttcaaacaa ttcagacatt
    26701 caatcatcca tcctcagact tcattttgaa agtttttcta ttataagctt tcgaatccga
    26761 aataatctat atgattatta taaagcagct tcactgcaca gaaatattaa tcttttgaaa
    26821 gatttccgga atccttggat ataatctttg tcagaatgct tgatagtatt aaagtcatat
    26881 gatatttatg aaagttgttt acgcaaatat ttcacacttt tttaagaata ctatgtacaa
    26941 taaaatcact ggcattccca tcgctcgttt tgggcagtaa attatttcag tggagtcgcc
    27001 gttggatcag cttcaggata agattattct catagaaggt aagcagaccg atcacccctc
    27061 tttcttttta agtttaaaga aacggggata taatgtggaa attgcttcca ccggtaatag
    27121 tgccattgag aaaattcaag agtccgatcc gatgatcgtc ttgatcgatg cggcttcaat
    27181 gcgaacagca agcaatcgga tcgtctaccg tatcaagaag cgcaatccgg acctgccgat
    27241 cattctgatt atcgatgaaa tgctcaaaca gaagaagatt tccgacaaag ccgatcttgt
    27301 tctttgttta ccgtttaccg ttcaaaaact tttgaatcgg attatgcttt ttgctcccgc
    27361 gaaaaaaagc acactcaaat gcgcgggtga cctggcactg gatacctccc gtaattgtat
    27421 ttatatcgat aatcgggtag tcggtgtcac tccgcgagtg tccaaacttc tcaatttatt
    27481 tatggatcgt cccggcgaaa ttataaaacg ggaagaatta ttccgagaac tatgggatac
    27541 ggactatttg gaagacatgc gatcgttaga tgtccacata caatggctgc gaaaggcgat
    27601 tgaggaaaat ccgaagaatc ctcaatattt gattaccgtc aaagggcagg ggtatatgct
    27661 cgaaccgaat ggaaacggga aaaataaaaa aatcgatcag tgcgctttat caccggtctg
    27721 ctgtgaaaag gaatcctgat ctcctgtcat aaggttcatg aatcctttgc gccaaatgtt
    27781 tataataaac attaataaga taggtcaagc gggactttaa ttcggctttc ggatgacaga
    27841 gagtcgccgg ttgatgagag gcggcaacaa gtccgaattt atccaccccg cgcataaaaa
    27901 atgcggaccg ctttcgaaaa gcaatgatgc gataagaaag ccgattcggg atcgttatct
    27961 cccgacaaag aggctcttgg ttgagcaaat gcaggtggta ccacggaaac cccttcgtcc
    28021 tgccggacgg gggatttttt aattgataga taagaggagc tatttatgct ggatatgaat
    28081 ctcatccgag aaaatcccga attggtcatc aaatcgatga aagatcgtca aatggatccc
    28141 gctccggtga atcgtgtgat cgaactggat atgacccgcc gaaagcttct ttccgaatcg
    28201 gaacaactga aagccgaacg gaatgcgact tccaaagaaa tcggtcggat caaggatgcc
    28261 gaggaaagaa acgccaaaat agaagcgatg cgccttgtcg gcgataaaat cgccgaattg
    28321 gacaagaaag taaaggaaac ggaagaggaa ttaaattcgc tgatcatgat tattcccaat
    28381 atcccggatc cgcgcgtacc ctacggtgtc agcgatgcgg aaaatgtggt cgtccgctac
    28441 gaagggaaaa tcgcggaacg agagtttgat ccgattccgc actgggattt gggaccggcg
    28501 ctggggatca tcgattttga tcgcggtgtg aaattgaccg gaagccgttt ttacattctg
    28561 aacggagccg gtgcccgtct gcagcgcgca ctcattgctt ttatgctcga tttgcatatc
    28621 cggcagggtt atgaagaacg gtacacgccg tttatggtaa aaagtgaaat actctacggg
    28681 tccggtcaat tgccgaaatt cagagacaat ctttatcatg atgccgaaga agatttttgg
    28741 atggtgccaa ccgccgaagt gcctctgacc ggtctctata tgaacgaaat tatcgaagaa
    28801 gacagccttc cgatgcgttt taccgcatat actccctgtt ttcgacgcga aaaaatgagt
    28861 gccggacggg atgtgcgcgg tattaagcgc ggtcatcaat ttgataaggt tgaaatgtat
    28921 tgttacagtc atccggataa atcagccgat gaactggatc ggatgttgaa tgccgccgaa
    28981 gaaacctgca gacaattgga cttgccttat cgaatcgtcg cccaatgcac cggcgacctg
    29041 ggatttaatg ctcggatttg ttatgatatc gaagtttggg cacccggctg taaggaatgg
    29101 ctggaagttt cctcagtttc cgatgacggt gactttcaat cccgcagagg agcgatccgc
    29161 tttcgggaca aagaaagcgg aaaaacccgt tttctacata ctttgaacgg ttcgggattg
    29221 ggtttgcccc gcacattgat tgcggtgctg gaaacctacc aacaagccgg cgggtccgtg
    29281 gttgtcccgg aagtgctcaa accttggatg ggtggtattg acgtaataga gaaacaacaa
    29341 taaatcgaaa gttttattat ttcttccgat ttattattgt ttatgattgt taattacata
    29401 atatccggag aatcaattcc caacagagcc aggcaattga tcattgtttg gcgtgcagcc
    29461 gcagccagcc gtaaccggta atctcggaca ggcggctccg cctgcaatac ggggcattga
    29521 tgatagaaat tattaaatgc ctgagccaga tcaaatgcaa ggttggaaat gaacagcggc
    29581 ttacattctg cagccgaccg ttggatttcc ctcggaaccc gtgaaatcag atcgatcagt
    29641 tccacttcga ccggggtcag ttcgtaagac aaagctgttt gatccggcaa tccgttttcg
    29701 gattttctta ggatactgtt tgcccgtacc gcagcatatt ggatatacgg agcagcttga
    29761 ccgttaaaat caagtgccgc ctcccagtca aaagtgacta tttttgcatt atcccgcgag
    29821 agcatggaat acttcagcgc accgagcgct actttttccg atatttccgc tttttcggca
    29881 tccgttaatg acggattttt ttcttgaaca atcgccaacg ctcgcgattt agcctcatca
    29941 attaaatcct gcaggaaaac aaccgttccg tcacgcgatg acatggtgac atttcccggt
    30001 aaattcacga tttcatatgc caaatgataa caattcttcg cccacgaata acccatcaat
    30061 tccaaagtct tgaatatctg ctgcagatat aacgtctgcc tgacatcgat gacatagatc
    30121 gaacgcgtca gatgaaattc ttcgaacttc cgaatcgcca acgacaggtc tttcgtcgca
    30181 tacaaagagg tgccgtcgga gcgcaagacg actaacgaac gatatttatc cttcgtaccg
    30241 cacaattcat ccaatttgac gaccaccgca ttttcggggc gttcgtcaac ggcgattcct
    30301 ttggcgatca attctttgac aatttctttg cccgaatcct caacatcact ttcaaaatag
    30361 agcttatcga accggacgtt caatgtttta taaatctgct cgaaaccatc caaactccat
    30421 tgacgggtaa cacgccataa ttcgacaatc tccggatctt ttcgatccca gcgggcaaag
    30481 agttcccgga tttcactttc tgcttcgggt tcttcttcat ggcgccggct tgcttccgca
    30541 tagatatcac ccatccagcg ggtaatatcc ccctcgggtt tctcgccgtt atgatatttc
    30601 agatagcacc acaaccattt gataacatgc agtccgatat cccccaaata attcgcccga
    30661 atcacatcat taccggcaaa atcgagaata ttacagaccg catttcccag aattacattt
    30721 cgtaaatgac cgacatgaaa tgctttatga gtgttcggct gggaaaattc aaccattacg
    30781 cgctcaccgt tgcgttgccc tctgccaaaa ttatcttttt cttccaacac cgtatcaatg
    30841 acccgctgag aataaattcg agtggtgaaa tagagattca aatatcctcg aaccgcttcc
    30901 gcacgttcga atccttcggg taactcaagg gaatccgccg tcatttgagc aatttcctgg
    30961 gcatgcaagc cgacatttat tttttgtcct ctggaacgtg catcatccgc cgcgacctga
    31021 aaaaaagaag tggatatccc ccaatttccg ctgaatggaa tccaggacca ggtaagcgat
    31081 ttcaccggat aattgtgagc ttcacaaatt ttccgaattt gatcctctat cagttgttgt
    31141 tctttttcaa acacggctcc tccactggtc attcttcaca tctcgaattt tcaataagaa
    31201 tcggacggat cggatctata tgaccaaaaa agggttgaac tttttctttc aaccctttcc
    31261 gatccggcaa taacattcaa aaagggattt ctccctcacg atagcgttac gctttttttg
    31321 tcgctgtcag tttctttatc aggcgactct tcttgcgggc tgcattattc ttatgaatta
    31381 cacccttttg agcggcttta tccaaagcaa ttactgcctt cttcaccatc tcttcggaat
    31441 tttcctgatt cgtttcgaca gccagacgtg ccttatatag agcggttcgg gtagatccgc
    31501 gaacaacacg attttggata cgtctctttt cgttctgtct gttacgcttg atttgtgact
    31561 ttatattagc catttcctta ttctcccaag gttcaacaca tagtagggtt ccggtcaagt
    31621 agacttttta tgcctatttt ttctcttccg tgcgcccttc gagggtatgt ccggaacttt
    31681 agaacgggca ttattatcct atatcaggcc tattttgtca aatccctttt cggctgaaaa
    31741 gtatgacgga tgatcaaccc cgctgtggca caaacagcgg tcactcccat gatcagttga
    31801 ttcatgtcca atcctcccac catcgccgta tccaaccgca aaaattcgag aaaaaaccgc
    31861 ccgaccggat aaacgatcaa ataaatcaaa aaaatatctc cgtctttcag ccgtttacca
    31921 aatcggtgat caacccaaag cagaaaaccc atgttcaata agttatagat taattcatac
    31981 aggaaaagag ggtgataggt cgcctgctgt tcaaatccgg gaatacgata ttgcggatca
    32041 atggtgatcg cccaaggcag atcgctcggc gcaccgtaca gttcctgatt aaaataattt
    32101 cccagtcggc cgattgcctg tcccaatgcc aacccgggag agattgtgtc ggtgaatttg
    32161 ctgattttga atccgtattt tctgcaaaac aaccataacc cgatatatcc tccgagaatc
    32221 gctccgggaa ttcccagccc gccgttccaa acctgaagga ttttgacggg gttgtgtaaa
    32281 taatagctta gcgtaagtcc cgaggatttg gaaggcaaaa acacatgcca aagccgggca
    32341 ccgataatac cggcgatcaa aagccaaaac agacaactcc aaatcgtatc cggtgcgatt
    32401 ccgcgctttt ttccggtccg gcttgcgaga agtgtcgcac tgaagactcc ggctaagaag
    32461 cataaactat aataatgaat aagaaacggc ccgatcacaa acccatatga ataaaattcc
    32521 aatggtcccc gcctgacttt tttgttggtc taattttctt tcaattcttt ccgcatatat
    32581 tgaatgcgct ggatcgccgt gatatgagat ccgatcgcta tcgcccacat ggcaataaac
    32641 gggatattaa acaacagaca gggaaccaag aaaagataac gttccagtcg tgacatgatg
    32701 ccgagcttca tatccaatcg ttcgatttcg ccttttgcac gggtataaga taccaggatc
    32761 gaccccataa tggcaatata cgccaccatc atagtcagat aatcctcatg aaccgcatag
    32821 taataaatca aaccgccgaa aatgaagaat tcggcatagc gatcggtaca ggaatccaga
    32881 accgctccga atcgggtgcc cttcccgccg gtggaaagcc gagccatcgt tccgtcaaag
    32941 aaatcggtaa ccgcaaaaaa taccaagaga atacccgccc aggtgaaatg tcccgtggca
    33001 gctaaatatg ccgcgataat atgcccggca cagccgataa ttgtcacggt atttgcggtc
    33061 agaccgatct tcagcagaaa tcgagaacag gtttcgccga aacccctgaa ccatattcga
    33121 cattgatcgc tgaaggtttt cggtttttca ggttggttcg atgagttgtt tgaaatattt
    33181 tcgttcataa tgtcttctgt ctccttttta tgcggcattt tctccgtaag aatccgcttc
    33241 ttcttcagac cataatactt cacgcgcttt cccgccgacc tgaatcggac cgaccacacc
    33301 aatctgctcc attcgatcca acagccggct ggctttcgga aaactgatat tcatttttcc
    33361 ctgtagatac gaagcgcttg ctttccgtgt ccggcaaacc aggcgaatcg ccgctttcaa
    33421 atccttttct tctttaccgt ttacttcctt ttccgcgtct tcgatcattt cttcccaagg
    33481 agcttccgct tcttcgacgg gttttggcga taattttttc cataaggcaa cgaccgaatc
    33541 aatttcctga tcggtcagta aaggtccctg gattcgaatc ggcacattca gactgggatc
    33601 gacaaaatac atatcccccc tgcctaataa tttttccgca ccctgtttcc ccataatcac
    33661 tctcgaatcg atcgccgaag caacatttaa tgcgattcgc gcgggaatgt tggtcttgat
    33721 tttgccggta atcactgttg tatccggtcg ttgggttgcg acaaccaagt gcattcccgt
    33781 cgcccgtgcc aacgatgcca atgtatcgat atattcctga cccaatttat ccgcgccttt
    33841 gacaatttcc gccaactcat cgatgaaaat cacgatataa ggtaatggtt tctgaccgtt
    33901 tcgaaccgca tattggttat ataaagataa tttgcgaact cccaccgtct caaataattt
    33961 ataacggttt ttcatttcct gaactgccca gcccaaaacc gaaatgcttc ggtcatattc
    34021 ggtttcgact ttaccaagta aatgaggcaa tccgttgaaa cggaaaagtt ccaccctttt
    34081 gggatcaatc ataatcaatc gcaatgtttc cggcgtgtta ttcatgatta gacaaaccgc
    34141 catcgaacgg attaaaatgg atttaccgga attggtcgta ccggcaatca acagatgggg
    34201 catctgttcc aaatcaacca ccaccggttt accggaaata tctcttccga gtgcgacatt
    34261 caacggtgat gtcttggatt gaaactcccg gctttccatt aaagggcgca gacgaacttt
    34321 cagggagtcc ggattgggga tatcgatccc gataaaattt tctcccggca ccggtgcctg
    34381 aatcgaaaga tttgccacgc ccatccgaac agccaaatcc cgttccatct gcgcaacctg
    34441 agcgacccgg attttcttgg ggattaccgt gccgtgactt ccgttctttt ccaaagcgcc
    34501 cggaacaacc tgatattgga taatcgcggg tccgatactg taaccgataa ctttcaccgg
    34561 agtgccgaac tcttccatgg cggattcaat ctcatcgatt cgtgactgaa catcaatatt
    34621 cttatttaaa aatcccattt ccggttccag caaattaatg ggcggcaacc ggttggttcg
    34681 cggcttgctt tttcccttta tttcatttcc gccgataacg gaatcgatcg gatgattcgt
    34741 aatgtttttt tccaatggat cgttttcctc tttttctata ttttctttca ttaatcgtct
    34801 gcgaaaaagt gtaacctcat cggaggatcg gatcggtttt gaagcgcaga aatgtgcaac
    34861 tttctttttt ataaaaaccg ccgattcgga caaagggcga ttcaaatcaa aaagaaaata
    34921 aaataggacg gcaatgattc cccataaaag gatcgtcgcg ggaatttccc cgatcaccgc
    34981 ggtaagctga ccgaataaag tatccccgat ttttcccccg gagaaatgat cactgcttga
    35041 aacgagtgcc agcaataaga aaaacaacag aataattaag ccgatttttt tcaacggaat
    35101 acggattttt acggttgtat accggctgag aaaaatgccg gttccgagcc cgagaaaaag
    35161 cacaacaaat atgattctcc ccgtcccgat ccaatcatca aaaaaattcc gcattctcaa
    35221 aataaaacgg ccgtcggttt ttccggtaaa gcccacccat aacagcaagc cggtcacaat
    35281 acagccgagc gcgaggatat ccggaattaa agattcgctt ttcagccgcg agcccgaagg
    35341 cttttggggt tcattctctt gcttgttctc tttttcaggc attctcttta tctgtcaacg
    35401 gataaacgaa tcaaatttta tttcaaacca aaaacacctt acaggtggga agaattatgt
    35461 tccttttcca tacgaataat cgttttcgcc agttcttcga ttgcgacccg ttgctttcga
    35521 tatagatcag cttcggacat cgccaacttt ttcgccactt ctttcacctt taaaccttcg
    35581 atgaatttca gatccaaaat attatacaga gtccactctc ccgaaataga ccgttcgcca
    35641 ctcggacgga tctgatcaat cgcttttttc aaaattgtac gcaaagcatt tgtcgcatta
    35701 ttgctgtcac cgttcatttc ctgttgaacg atatccaatc ggattaaggg attttcggtc
    35761 aaccgtggtc cgccccaata atgggttaac gcatctcgaa cccaggtgct gacttcttcc
    35821 agctcgtagt cggatttttg atctaatact tccgcggtgt ttaataaacc gatcttacga
    35881 tacgaaccta acttttcatc gttggagagt tctcgtaaaa cattgaatgt tcgtcccaaa
    35941 taccaacgtt gccataaaac aatccgaatt ttatccgccg caacttttaa agccgattgc
    36001 ggttccgatt cctcgccggt gacagccggg tcagtaatac cgataatcgc caacaaccag
    36061 gttttatttt cggtaagatc atgatagaag accggcaaca aaatgctgcc gtcatccgta
    36121 atcggttcgt cggtaaatcc ctgaagcgct tcgatttttt cggaaaaaac aatcggatca
    36181 gatagactcg tttcaccgac atgaatcaga ttctcaacat tgcccatgga atccgttacg
    36241 gcaacaaaga cccctttcgc ctggtatata tcgcataggg cgctgataat cgactccaaa
    36301 taggtttcca gctccgagga aaatatcatc atattttcga gaccggaaac aatttgatag
    36361 tcctgatttc cgtagccaga aatgctgttt ctttcaaaca acggcatcaa taaggttatc
    36421 agatattcca gcactacgat cgaaacgacc atcaaaaggg acgtccatcc gcctacattg
    36481 atattcaacg ttcccgagct gcgccggatg atcgttacga gtcccaaagt cagcccggcc
    36541 gtcaccggtc cgcgtaaaat ccattccatc aggcgcaagc gaaccactcg ataagaccag
    36601 gaaacacaaa aagtgataac ggaataaccc aacaaaagca ccatcaacag gatcagaatg
    36661 ttggcgatta aattgaccag ccaaaatccg agttcatgcc gcagcagaaa gccggatccg
    36721 taaatcatca aaggaaatgt cccgaccaaa actcctaacg cgctgacaat cagatagagc
    36781 atccttcgac ggctggtcgg tgttcgggtc cgcttaaaag catgataaaa attgatcagc
    36841 atcgcactca aaaggacaaa cagccaaatc caaaaataat ttaatttctc cgtcgggatc
    36901 atggagattc caagatttcc gacccgtcgt gttccgtaaa aaagttgatt cttccaaagc
    36961 aagaccagaa aaatcaccga gaaaatataa gtgacggcgg tcatcttttt cctcagcttc
    37021 cggattgttt ttcccgtcat ctgtaaaatt acttgggaaa aatgaatcat cgtcgccggt
    37081 aaaaagataa tccccgacca atggatttgc tgccaaagca attgattttg gtcggataat
    37141 gtcgtcagcg aaagcgcatc agctgtataa atcacgctta aagctaaaag aattaatgag
    37201 aaaacccgtg caagtcgttc tttttgaata aaaaataaag aaaataacaa aagtgaaaat
    37261 gacgcaatcg caatggatgc ggtcaaaatt tcactgatcg ttcgaatgat aggcaaaatc
    37321 caattaatca tatcgttcgc aggctaccgg atattttttg caaagaataa cgcaatctcc
    37381 atattttgaa aataaaaatc ctgatacccg caaaattgaa acccttttga aagcagaaaa
    37441 tgaatcgcag gatcattttt cgcctgaaca gtacaaacaa gacggggaat tcccactttt
    37501 accgcgtttt cctgaatcac cttcaagagt gcagatccta ttccttttct gcgataatcc
    37561 ggtttcacac ctcctgtttt taataaaatt cgattcgtat tttcatcttg ttccaaacgc
    37621 acaaaaccgg ccacttggtt gtcatattgt gcgacaatga cttcctgacg cttgaccatc
    37681 ttttcgatag cctcttctcc acctccttgg aaaggaacat ggacggtctt cggcaacttg
    37741 atattctgaa agacaatcgt atatgaatcc aaatcatcga tcatttgcga tttccataca
    37801 taattgcttt caaaatccaa gtcaattttt gtaatcaacg ggatatctgc catcgacgcc
    37861 atccgtattt ctatttcagc catgatttat cgatatcctt aaccttttcg tttctttgat
    37921 tttaatccta ttattgagtt attactcttt atttaaaatc cgaatggcaa catcgcaagg
    37981 tgttaattgt tgattcgctt tatttacaac cgtcaatcgc aaaatataat caccgttcgg
    38041 aatttcactg gtcgccaaga cacctaattt tccttcaacg accggcatat tccccgcgga
    38101 aatagcgtgc caatcggtag gatcactcaa agaggcataa tcatatttga aaaaacccat
    38161 atccagaata ttgaccgttg ccatcaaatt ataaataccc gatatttctt cgccgggttt
    38221 cggtgcgatc cattcgattt ggccgggaat acatccgccg ccggtaacat tctgagcgac
    38281 agcggaagaa gcattcggcg aacccgtttc gacgcccgtg ccgctcgttg ccacaatatt
    38341 caaatcgacg ccatccgtgg tcgccggcag cgattggatt cccgttgctt gcggaagagc
    38401 cgaaataata aaattcacac agataaattc ggcaatgatt aacaaaaccg aaaggatgac
    38461 cattgccaac gaagtattta atttatgctg ggcaaactct ctttccaatc cgaaaattgt
    38521 cgaacgccat tcgataatcg cacgaatcag cttccttccg taaattaccg cagtgattaa
    38581 caaaacggta taaatccaat tctgataatt cactaaaaag ttcaacaagg attgcattga
    38641 tttcttttcc ggtcatcaca tatcggggag aatttcgtat aaaattcgga tgattttggg
    38701 accgataata tcggcgatct gtaaaacctc ttgatgatcg gctttcattt ctccgtcatc
    38761 gctgcattta ttggttatgc cggaaagccc taacacccgc attccgccat gccgtgccac
    38821 aattacttcg ggtaccgtgg acatacctac cgcatccgtc cccacggttc ggaggaaacg
    38881 tatttcggcc ggtgtctcaa aggaaggccc gctcaaccaa gtataaacac cttctttgag
    38941 atctattcct gttcgaatgg cggcttttct tacgagagag ccatattctc ggtcatacgc
    39001 ttgtgacatg tccgggaatc ggggtccgaa ttgatcaaaa ttccggccgt tcaacggatt
    39061 gaatccgctc atggaaacga agccgatatg atcggtgatc aacatgacat cgcctgcgga
    39121 gaacgacgga ttcaccccgc cggcagcatt ggtcactatc aaactcggga tcttcagaca
    39181 ttgcatcaca cgaatcggaa aggtaatttc tgccggagaa tatccttcat aaaagtgaac
    39241 ccgaccttgc tgaaccagaa ccatccgccc ggcaagctct ccgatcacaa gccggccctt
    39301 atgtccttcc acggtcgaaa gcggccaacc gggaagatcg cgataatcga taatcagcgg
    39361 atgcttcact tcatccgcca aaccgcccaa tcccgagccg agaaccaaag caagttcaat
    39421 gggcgttttt attttttctt tgattatttc ggcagctttt acataatcgg catattcata
    39481 agttttcatg gattcatctc cttttcccct tctaattata cagggaatcc ctcttcgaac
    39541 cgcagatatc cacggacctt gcaaaccttt cggagtcttc caaaaatcgg aataattctg
    39601 cgaaaaccga gaatccttgg ggttcgaaaa gctttctatc tctgaaaaga aaagggtaca
    39661 aacggttttt ccgctgtacc ctttttttat ttttttgata atcgaattac ttgttcacgg
    39721 taccgttata atttgaattt cgtttgcgtt ggaaatattt gacgatctca acaatcggga
    39781 taaccgagat tgccaacgcc atggccgtca tatattcgtt gagcgatatc gtctcaaact
    39841 cgaataatgc agcaattgaa gggatttcga tcacaccggt tgtcagaatc agagcgatta
    39901 ccatcgctaa aatcaggaat ttgttgattg aatgcatcga aaaaatagaa ttccgacggg
    39961 agcgcatgtt gatcgaatgg aagatctccg ccatggataa ggttaaaaat gccattgtca
    40021 ccccatccgg gctttgggtg atttcccatc ttccggcttc cagataatga ccgacgaaat
    40081 aactgacgag ggtcacaatc gcaatcataa cgccctgata cccgatatcg aatcccatcc
    40141 cgtccgcaaa gatatgcgag tttggatctc tcggtttcct tttcatcaga tctccttcag
    40201 ctttttcaac ccccagcgct aaggcgggaa tcgaatccgt aatcaggttg atccaaagaa
    40261 tatgaaccgg cctcaaaatt tgaaaaccga tcagcgtagc cacgaaaatt gcgatgactt
    40321 ccgcaaggtt agaagaaagc aagaattgga tcactttcag aatattgtca taaacccggc
    40381 ggccttcctc gacagccgat acgatggttg caaagttatc gtctgccaaa accatatccg
    40441 cgacattctt ggtcacatcc gttccggtaa tccccattcc gacaccgata tccgcacttt
    40501 ttatggaagg agcatcgtta acaccgtcac cggtcatcgc ggtgaccatc ccttttttct
    40561 tccacatttt tacgattcgg gtcttatgtt ccggttgcac tcgggcatac accttgatat
    40621 tttcgatcat tttctcgaat tcttcatcgc tgatatgatc caactgagct ccggtaattg
    40681 cctgatcttc gctctcaaga atgcccaatt gtttcgcgat cgcgatcgcg gtcgctttat
    40741 ggtcacccgt gatcatgatc ggcgtaattc cggcttcgct gcattccttg atcgccccga
    40801 tcacttccgg tcgaatcgga tcaatcatcc ccattaatcc caggaaggtc aaatcgtttt
    40861 cgatttcttc cgcttcgtat gacttcggtt cggcgggata ttccttcatc gcaccggcaa
    40921 gaacccgcaa agcatttgcc gccatcgcat tattggcttc catgatctca tttcgtaact
    40981 catcggttaa cggaatcaca gatccgttat gaagccattt ggtacatttg gtgagcactt
    41041 catcgggtgc acctttggta aattgaataa ttttgccgtc atgccggtga accgttgaca
    41101 tcatttttcg tttggaatca aaaggagctt caccgattcg agggtattct tttttaagct
    41161 tatttttgtc aaggccgttc tttgccgcat agattaccaa ggcggcttcg gtcggctctc
    41221 cggagacttc gatcttctcc ggattgttca aatcctcagg agaagtctcc aattcggcat
    41281 cgttgcataa agccatggcg atcgagagca actgcatatc atcgccaaat gtatcaacta
    41341 ccgtcatttt attttgggtc aaagtgcccg ttttatccga acagatcact tgcgcggacc
    41401 ctaatgtttc cacggcggtt aatttccgaa tgaccgcatt tcgacgagac atgttggtca
    41461 caccgaccga aagaacaatc gtgacaaccg ccaccagtcc ttcgggaatc gcagcgacag
    41521 ccaaactgac cgcgatcata aacgtgtcca aaataaccgg acccgagaaa tttccattgc
    41581 gaattaaggt gaaaataaag ataaaaacac aaatcccgat taccagtttg gtaaggattc
    41641 cgctcaagtc gtttaatttt ctctgaagag gagtcagttc attttcggct tcacccaaag
    41701 caccggcgat ttttcccatc tcggtgttca ttccggtagc cgttaccacc gcttccccat
    41761 gtccgtaaac gaccgtgctt cccatataac acatattttt tcgatcaccc agcggaaccg
    41821 tatcggaatt ttccaaatgc aggatatcga taaacttact caccggttct gattcaccgg
    41881 ttaacgagga ttcttcgatt ttcaaactgg cgctgctgat aatccggccg tcagccggga
    41941 cggcatcgcc ggcttccaaa atgatcacat cccccggaac taactccgag ctcggaatga
    42001 ccacttttct cccgtcccgt aaaaccttgg aagtggctgc cgtcatcttc tgtaatgctt
    42061 caatcgcttc ttccgcttta ttttcctgat aaacgcccaa aaccgaattg atgactacta
    42121 cagccataat aataaataca tctgcgatgg attctccccg aattgtcgaa gtaatcccgg
    42181 aaatgactgc ggcaacaatc aacataataa ccatcgggtc ggaaacttgt tctaacaagc
    42241 gttgccaaat agtcggtttc ggcggttgaa caaggacatt cctgccgtat tccgacagtc
    42301 ttttttcagc ttcagcttgt gtcaaccctt gtggcgaact ggaaactttt ttaaaaactt
    42361 catcggtgcc aattaagtaa taatccataa aatttgtcct tttcagaata ttctgtttga
    42421 ttctataaaa aaacaagact catgaaactt taatagaagt ttcatgagtc ttgttatttc
    42481 tgattgaccg ggtttttggc cgtgctgacg gtttcctcac gtacgaatgg cgcacgttaa
    42541 ctactccctc atggataatt cttctctatt atattacata atctttaaaa cagtcagagc
    42601 gttttcataa taaataatta tcaattctat ttatgaatcg aatgaatcag gccgatcgta
    42661 aatcgttcag aacatcttcc agttcgggag gtaggggcgc ttcaaaagtc atcggaaatt
    42721 ctttaccggg caacagaatc gtcaaacgtg ccgcatgaag aaagtgtcta tgaatcggca
    42781 cggatttttt tcggtaccca tacaacacat ctccgacaat cgggcatttc agaaaagcca
    42841 tatgaacccg aatttggtgg gtgcgtcccg tcaaaggatg agcctcaatc agggtatggt
    42901 tgggaaatga ttctttggta aagtattcgg ttacggaatc ccgacctttc tcggcgggta
    42961 atacagccat tctttgccgg tgaatcggat ctcttccgat aggcgtctcg atccgcccat
    43021 tgggcgtcgg cggtttgccg tctaccaacg ccagatatcg cttatcgatt ttatgagatt
    43081 taaattgctt ttgcaaaaag atatgtgcct gttcattttt tgcgatgata atcacccctg
    43141 aggtatctcg atcgagtcga tgaacgatcc cgggacgaat ttcgccgcca aatcctttta
    43201 aatcttgaca gtgagccaat acggcatgta ccaaagtccc gctttcatgt ccggctgaag
    43261 gatgaacaac catgccggcc ggtttattta cgacaataac ctgagcgtcc tcatacaaaa
    43321 tgtccagcgg aatattttcc ggaatcagct cggaagtttt ttcttccggc aatgtgattt
    43381 cgatttcctg acccgaattc agtttaagtc cgcttttgcc cgccggcttg ccatcgaccg
    43441 atatccgtcc ttccgtaata aattgctgta gacgggaacg ggaataatca ggcaatacct
    43501 cgaccaaata atgatccaac cgcatcaatt cggaatttcg atattgaaat cgcagagttt
    43561 ttccggagga ttgaagtcca tgatccattt taaggctgct tcccgtctcg ggataattca
    43621 gggatttggg aaccgttcga aacatccgga tccgccggaa cccggctcgt attgtcggtt
    43681 atcgacccga tcggggagga agtttcgaaa gattttcctt ctttatttcc ttcctttttc
    43741 tccgcccgct cttccgtata ccaaataccg attaacataa caatcgcacc ggaaacaatc
    43801 gcaaaatccg ccagattaaa aaccggaaaa ttcccgatcc aaataaaatc cacgacatat
    43861 cccacgttcg gattgatccg gtccagcaga ttgccgattg ctcctcccag ctgtagaccg
    43921 agagcgattt gccaaaaagc cggcccgtta aaaacgggtt tatagaagaa gatgattatc
    43981 aatatcacca ctaatcccag cgatgtcaaa atccatccca ttccctgaaa cagaccgaaa
    44041 gcaacgccgg tgtttttcca atgaataaaa cggaaataag gttccaacca agaaatgggc
    44101 atccaggttt cgcgaatatc aagattcgta cgcacgaacc atttcgttaa ttgatcaata
    44161 aaaattacac cgagcgcaat agaaaataat ttcagataaa cttttccccg tttattcaat
    44221 caattcctcc gactctcaca aacactaagc ccaattatat atcaaaaggg accggccgga
    44281 tttcggctca gtccctttgt ttatattctc cctcttacct gtttttacga aaaataatct
    44341 tttctttccg gcatctaatt gatcggaaaa tattttcgga aattcccctt tttcgcttac
    44401 ggtaaggaga attcataagt gatatcgacg gtcaccacaa tttttaaact gccgctttcc
    44461 aaaggcgccg taccgccgta accaccgccg cccattccgt acatggcacc gtaatcggtg
    44521 ggataatccc gaacctgaaa agatttaata ttgccgactg tcacacctaa tttctgcgca
    44581 atctgctggg cattggcatt tgcgtcatcg atagccagat ccctcgcttg catatacgta
    44641 tcttccctca aagaggaatc ataagagaca ctgttgatgg agttgacacc gttattgata
    44701 caagcatcta aaacatcatt caacttggaa atatcccgta atttgatctc aaaaccataa
    44761 aaaacactga aaatatattc atccattccg gcggtttcat cccgataata cttttgggta
    44821 ctgtataacg aataagatgt gatcttgata tcctcttcgg gaatacccat ttcagtcaaa
    44881 gccgacttta ccagcttaac actcttggta ttttcatcaa gcgcctcctg aacagattct
    44941 ttttcggtgg agatggatac attgatgatc gctatatcgg gtttcagcag gacggttccg
    45001 gtcccgctga ccgtcatggt ttgatcggat tgggcctgaa ccgaaccggc catacctaac
    45061 gagagaatca tcatactgag aaaaacaatc attaacaagc tgtttttttt catttcgact
    45121 cctttttatt tctaattttc aatttactgt tctattttga ctcttaatcc gtaagtgcaa
    45181 attccatatc gacactgacc gtcacagtca gaaatcccga atcgatcggc gctgtcgata
    45241 catcttttac caatttggtg gaaataagcg gaatttcggt attatcaagg acggaaaagg
    45301 ttagcagatg atcaaccttc ttgcccaatt tttcggcaat ctgttgggca ttcacattcg
    45361 cgtgatcgat tgccaggtcg cgcgcttgag ccaccgcttc actccgtttc gaataatcaa
    45421 aatttacccc atataagttg ttcgctccgt tttggatgca aaaattcaaa acatcattca
    45481 gcatagacaa atcccgaacg acaatttcat aagccaaatt taccttataa atatattggc
    45541 cggcaatagg attcccgtct ttatctgttt tcgacaatga atacagaata tactccgtcg
    45601 ttcggatatc atcctctttt aatcccattt ccaatagtcc ttgttgaatt gcgagtgcat
    45661 tctttcggtt ctcatcgatt gccgttttac tgtcgtcggc ggttgtagcg atggcgatat
    45721 cgattaacgc caaatcggga ttcaaggtca cttctccggt acccttaact ttgatgatcg
    45781 ccgggctttc gttttgtgcc gaaactttga aaacagaaat accgaacaga gcgatcgtcc
    45841 cgataaaaag gaacaaaatc tttttaaaat tcattcataa gcctgccttt ccgtgatccg
    45901 caatgggatc aatttcgtat tcttaacatg ggatacgttt tttccgccgt taagttccat
    45961 agcatctcgc ttttattagt cttttggcat tgaaaatatc gcaattcaat ataattttaa
    46021 ttcggaatga tatatcttgg ccggtgatac ttcactcaca tgaaacggag catttggatc
    46081 gggaaatgat gacctatttc acaggaatcc gcttgctgcc ctgttataat tggcacagaa
    46141 atatcttaga ggcacgaagc cgaaaagtta aaggggatat atgttggata aaatcgatgc
    46201 gatcgaagct cgttacaacg aactcagtaa tttgatcgca gaaaatgtgg aagattacca
    46261 aaaaaccgca gaatggatga aagaacgttc tgaaattgag gagatcgtcg aaaaaggacg
    46321 ggaatataaa gatacattaa aacggatcga agaagacaaa gcgttggaaa aagatcccga
    46381 gatgagtgaa ttggctgcaa cggaattaga tgatctgctt cccaaaaaag aaaatttgga
    46441 gaatgagatc cgctcgatgc ttcttccgaa agacccgcgg gattcacgga acgtgattat
    46501 ggaaatccgt gccggtacgg gcggagatga agccgcgatt tttgccgccg atttattccg
    46561 catgtattca cactacgcgg aagcccgcca ttggcatatc gaagtgatgt ccgcaaacga
    46621 aatcggcttg ggcggatata aagaaatcgt ctttatggtc aagggaaagg gcgcttattc
    46681 acggctgaaa tacgaatcgg gcattcatcg cgtccaacgg gtgccgatca ccgaatcaca
    46741 gggaagaatt catacctcag ccgtctccgt tgccgtatta gccgaggtcg aggatgttga
    46801 tattcaaatt cccgaatcgg atattcggat agaagtttac aaatccgccg gagccggcgg
    46861 gcaaaatgtt cagaaaaact ctaccgcggt ccggttaatt catctcccga ccggtatggt
    46921 cataacctgt caggatgaac gttctcagtt acagaataag acccgggcga tgagtatttt
    46981 acgtgcccgt ctttatgata tggagcaaca gaagcgtcag gagcaattgg atgccacacg
    47041 tcgttccatg gtgggcacgg gagatcgatc ggaaaagatt cggacttaca attaccctca
    47101 atcacgggta actgatcatc ggattaatgt ctcttccttc aatcttgccg gagtgatgga
    47161 cggcgatttg gacgttttta tcgatgaatt aattatgcgg gaacaaacag aaaaattatc
    47221 ggtttcagaa ggggcatgaa cggttccgat tcggtcggtg aatggttaga ttgggcacgc
    47281 cgggaattac aaacgagcgg ttacagcgat acgccttcca ttgaagccca atggattttt
    47341 gaaaaagtga ccggagcgca tcgtccggaa ttgctttctc atccggagaa gccgctttcc
    47401 gataccgaaa taacgaaaat ccgagacatg gtagaaaaag tcaaagaagg cgttccgcta
    47461 ccgtacgttt taggagaatg gagctttttc ggtaatactt ttcaaatcac gccggacgta
    47521 ttgattcccc gaccggaaac ggaaatattg gtcgaacagg ccatcgaatg ggcaaaagat
    47581 cgcttgatgc cgatccgatt tgccgacgta ggaaccggtt gcggctgtat cgctgtttct
    47641 ttgctgaatt attttaagtc ggctcgcttt accggtgcgg caatcgatat ctccgatgcg
    47701 gcattaaggg tcgcccgccg gaatatcgat cattatcggt tgaatgaggt tcttttcccg
    47761 atccgcggaa atcttacgga atgtctctct tctccattgg atttagtctg tgccaatttg
    47821 ccctatattc cctcggaacg gtgccggtca ttggacgtag cacaaaatga accgctgctc
    47881 gcactggatg gcggagaaga tggttttgat ctttatcgca tgttgttacg agacttgaca
    47941 gggaaaatgg caaaggaatc attgattctg tgcgaaattg atttttctca aaaacgagaa
    48001 gctttgtccc tttcacgcca gctgttcccc gatgcatccg ttacaataaa agaagattac
    48061 gccggccttt cccgtttatt aatgattcgg aaatcatgac tatgaaaaaa atatacgata
    48121 ttacttctcc tcagatacaa cccgttgcca ttcaacaggc tatcgatgtt ctgaattcgg
    48181 gaggtgtggt tatttttccg accgatacgg tttatggatt ggctgcttcc gcctttaatg
    48241 aaggagcaat ccagcgtctt tatcggatga aagaacgtga tccccaaaaa tcaatcgcgg
    48301 tattgctcgg aaatatctcc caaatccgat taattgccgc ggattttccg gaaagtgcaa
    48361 gaaagcttgc cgaacgttat tggccgggcg gattgacact gctcgtttcc aaactggata
    48421 cgttgccgcc ggcactttcc gccaatgaaa aaatcgggat acggattccg gattatccgg
    48481 taatccagga tctgattatc cacaccggcc ctttggcgac aacatccgcc aacctttccg
    48541 gtgaacctcc cgcaaaaaaa ttgtccgatt taccggactc tctgctccgg caagcggatc
    48601 tgatcttgga tggcggaccg gtaaaaggcg gaatcgcctc aacagttgtc gattgtacgg
    48661 ttcgtccgct tctggttctg cgggaaggtg cgatttcaaa ggaagaagtc gccgcattcg
    48721 attcggagac ggaatgaatc ctaagcaatc cggaattccg atgcgattga gaatctggat
    48781 tgttttgtgc atgttgatca tttccggctg ttcaaagacc gattctcatc ggccattgag
    48841 cggcggaatt accgatttat ttactttgaa gacggtgaca atgccggcgc tggcgacttc
    48901 tcgccccaca aaaacagcca ctccgacgcc gacttccgaa aaatccctca ccgcaaccat
    48961 tactccgact gagacttcca caccgctccc gatcgttacg ccgacttcct tttttgtcga
    49021 catatccgct tatccatttt ccgcaagtga taaattggaa atggttgatg acatcaccat
    49081 tccggatgga accatgctga aacccgggca aatgtttatt aaaagctggc ggatcaagaa
    49141 cgtgggtaat aaaatttgga ataacaatta tcggcttgca ttggcataca gcaatccctt
    49201 caataccccg caaatgactc acgcaatctt tattcagccc accgagttga ttgactttgc
    49261 gatcagcact tggaatccgc ggcaatataa tgtgggagtg aatgatacgg ttgatttggt
    49321 tataccgctt caggctcccg agcaagccgg cgattatatg gttgagtttt ttctgattaa
    49381 ttcggacgat gaaatcgtac cgccaaagat ctggattcga ttttcggtgg aattattgcc
    49441 gcaatttgcg acccaaaccg ccgttgcgat cgcagaatta actgagacgg cgactcctcc
    49501 ttccgaaaga gtccgcgttc cgacacccac tccccaacct ttcgactgga ccggaacctg
    49561 gttgatccgc aatccttatt cggataccgg catgatcccg gttcaggctt ggttgacgca
    49621 atccggagat accgtaaccg gctttttcta tgatacagac gaagagcctg tgctcattga
    49681 agggaccgtc gatcgggaaa atcagggctt tatcggaaaa tttgctcaac cgtggcagaa
    49741 tcgggcggtt ccggtaaaat ggcgcatgct gattaatcgc aaccagtttt atgcggtcac
    49801 cgacagcggt gaaacggaat tcggcacgat ttgcggcggg cgcaacggga tggcttttcc
    49861 cgaacattgt tcgatgccgc ccggaatttg aggtgctgat tcaatgattt ggattccgaa
    49921 cgaaaatacc gaccccgcgt ttaaccttgc cgccgaagaa gttattctga gttcattgaa
    49981 tatcaaagaa gaaactcttt tcttttatat taatgagccc tcgattatta tcggcagaca
    50041 ccagaatacc cgggaagaaa tcaacgccga attcgttggc gaacagagaa tccatattgt
    50101 tcgccgcctg tccggaggcg gtgcggttta tcacgatttc ggaaacctct gttatagctt
    50161 catttatcct ttcccggatc gattaacgcc ggattttgcg cttttcaccg caccgattgt
    50221 ccaagcattg caaaatttag gtgtaccggc tgaattgagc ggaagaaacg atattctggt
    50281 tgccggcgcg aaaatttccg gaaatgcata ttaccataat cgattcggat cggtctgtca
    50341 tggcacattg cttttcgatt cagatatggg catgctcgaa cgagcactcc ggccgaaacc
    50401 cgaaaaaatt gaacggaaag gggttcggtc agtccgtagc cgtgtaacca atctgaaaga
    50461 ttacttaccg gacataaaag atgtaaagga attacgtcag gcgattttat attcgatttc
    50521 tcggactcag cccgacttca aagaatttcg actcgcagaa aatgatatcc ttaaaatcga
    50581 atccttggcc gaatccagat atcgttcgga tcggtggaac ttcggaaaat cgccaaaata
    50641 taattatcgg gtatgcaggc aattcagctt cggagaagtg gatgcccgtt tatgggtgga
    50701 agacaatcgc atccgagaaa tacgattttt cggtgatttc ttcagttccg aagatccgga
    50761 tagtttttcc gagaaattaa tcggaatccc gtttgaatct caggcgatcg ctcaagttgc
    50821 acaacaatcc gacatagaaa agttattccc gggcatatcc gcctttgaat ttgccgattt
    50881 cttaaccggc aaacgcagtt aggatgattt ttctttttta tactcacaat ttaacggagt
    50941 tgtcgtaaat aaaggatgtt gatttagcgg ttaatcgttt cgaatcccat cgcccacaaa
    51001 aggcacgaag atcacatttc cgacatcttt ccgggttaat ttcccatttt ccttgaccaa
    51061 tttccagaga gtctgacttc tgttccgcgg accgatcgga atcaataaaa ttcctccttc
    51121 atccaattgt tcaattaacg gcggaggaac tttttcagcc gccgcggtaa ccacgatcgc
    51181 atcatatggc gcatattccg gccagccgta atatccgtca cctaatttta actttacgtt
    51241 ggtatagcct aactcattaa gaattttttt agctctttcg taaagctgtg gaatgatctc
    51301 cacggagaaa acatccccat atcccaattc cgccagtaat gccgtttgat aaccggatcc
    51361 ggttcccact tccaacacct tatcccccgg tttcaattct gacaactcga tcatttgtgc
    51421 caccaatgaa ggttgggaaa tggtttgccc gcagccgatc ggtannnnnn nnnnnnnnnn
    51481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51541 nnnnnnnnnn nnnnnnnnnn nnnnatcatt tgtgccacca atgaaggttg ggaaatggtt
    51601 tgcccgcagc cgatcggtag ggcacaatca tcatatgcct gatccgcctc acgagacggg
    51661 acaaacttat gtctcggaac gatcttgcag gctcgtaaaa tatccggatt ttttaaattc
    51721 atatatttcg cggcaaaatc aaaaagttgt tcccggtcct ttttatacgg atcctcttcg
    51781 caatggctat tcttctcttt cgagttcagg caacattttg aaattataaa atgaagcgga
    51841 attgcataaa aagttaggca aatatttcgc aatttttccg cattttcgca aagatggaag
    51901 cgggatttca taatcttttt ctttcgataa gctgttttgt taatctaatt atagggtact
    51961 cgcacgcgaa tcgatacagt taaaattttt gaaaattatt cgattgattt caaaataaaa
    52021 aaatccgctt ctttcaaagc ggatttgatt tttgttgctt taaacaacgg gtgtttgact
    52081 ctcccaaaac accgaaattg tctttttcac atcatcgtga atcgcttcca tcgcccagga
    52141 ttcgatatgt tcaaacagaa ccgtgccttt ggcttccgcc aaatgctttt tcacattatc
    52201 ggctcccgca cgtgccatat acgtgtaata gttgattttt cgtacaccgc gggcaatcac
    52261 ttttcgataa tcatcgtgac tgacaccgga tccgccatgc atgacaatcg gttttccggt
    52321 cagcttgcgg atttttgcca cccgatcaaa atccaatttc ggttctttca aataaattcc
    52381 gtgaaccgtc ccaaacgaac aagccaaagc gtcaattccg gttcgttcga caaactcttt
    52441 tgccgattcc ggatccgtat aaacggatgc cgggtcttca tcatgatcca ccgaaccttc
    52501 cgctccgtgc atgacgccga tttcggcttc gaccgaagct cctttggcgg ctgccaatcg
    52561 aatcactttt tgcgtattcg caatattttc ctcaaaaggc agagtggacc cgtcatacat
    52621 aaccgaagtg aatccgagat taagcgcctt ttccaaatag ggaatatcca ccccatgatc
    52681 cagatgaaca cagacgggaa cagatgatcg ttcagcctca cggagtaaaa tcggtcccac
    52741 tacttccaat ggcatatagc tttcatggga ttgggcatgc attaaaatga ccggagcatt
    52801 cagctcttcg gcagccgaga gaacccccaa aatacttgca agattcggtc cgttgaaccc
    52861 gccgatcgca catgttcgct tttcagcgat ttccaataca tctttcaacg ttaccagcat
    52921 ggattaatcc tcgaatttca tttatcagaa taattttatc ggtcttaatt acgttattat
    52981 tttacagaat ttatttaatg ggtttcaggt ccttatacat tctgcgtaaa gcccaggttc
    53041 gggttgccgc ttcacgggaa attgccaaat tcatcttgga agttcccgcc attctttccc
    53101 gaaaataaat cggaatttct ataaacttaa aacccaattt tgcggcgaca taattcatct
    53161 ccacctgaaa catgtatccg ttcgctttaa tcctatccaa cggcattgcc tcaagtgttt
    53221 ctcgcttcca aagtttaaat cctcccgtaa catcccgaat ggataagccc agaatcgcgc
    53281 gcgcataaaa gttcccgaaa ccggacaacc attttcgata gaaaggccag ctttcatcca
    53341 gttttccgcc tttgacataa cgggaaccga tcaccacatc acaatctttt aatgctgccg
    53401 ccataacagg tatcttttca atcggatgag agaaatcggc atccatttga ccgatgtaat
    53461 ccgccccatc cgccatcgcc atacgaaatc cctgattata agcggtgccc agcccgagct
    53521 ttcccgatcg atgcatacat ttgattcgat tgtcatatcg gacagccagt tcatccgcca
    53581 attttccggt tccatcaggg ctgttatcat cgacaatcag caaatgaaga ttgtccatgg
    53641 gaagggcaaa aatcctttca accaagagag gcagattttc gtattcgtta tatgtgggaa
    53701 tcacaatggt cgttttcatt tttccttgtc tgtctttcat tctgttcgaa aagacaaaat
    53761 cgggatcact ctgcgattat aattattttt cgattattat atcgtagaat ttatggatga
    53821 gcggacgatc caagaaaggg tctgccgctc agacaagagg aaataaataa ggcagaaggt
    53881 gttcgaagga aagttatgcg gtctttaagt aaaataatta caaccatact atcggcgatt
    53941 gtggcatgca acgccgtttc aattcctgtt ttttcacaga atggacagac agatccgatt
    54001 cccgtcgaag tgaatcaaac aggcagtgcg tccgccgaat ttttatgccg gccggacaac
    54061 tactccatga atcaggatta ttgtgtttca ttgggtcctt cggaaaaact gacaaaattg
    54121 gctgtggaag gaaaaagcgg cgcaaaggta ccttttaatc cgcagaaaat cgatccgtca
    54181 ttatcaaccg ttccgtataa ttatgcgaaa ctcaatttgg aaaatacgga atcggcacct
    54241 ttatacaata ccgttgaaga tgcgatcgcg ggaatcaatg caatcggaga aatacccgcc
    54301 ggcggaattc gatatgtttc ctatatcaat cgggtcgata cagctgacgg acactatgtc
    54361 caggcaaaaa acggttcttg gctgcgtgcc tcaccggctg ccctcccgac cgcaacatta
    54421 ggcaggactt tcacggctac tccgtccaat aatttcggat gggtcttcga aggttgcaat
    54481 ccttatcttg aacccaacta taattcgggt gttaacactt ctgtgtggtt tgatcgagaa
    54541 caggttctgg aagttctcga gacgactcaa gataaaaata ccgcctggtt caaagtcgga
    54601 gagaaccaat ggttagaccg gaaccatttt cgctgtgcct atatcaatac gacaccgccc
    54661 gaaggggtta caaacggacg ttggatcgaa gtagatttgc atgaacaggt tgtcatggct
    54721 tatgaaaata attcgctggt ctttgccgcc atggtagcaa ccggcatgaa acctttctat
    54781 accaagccgg ggatttttca aatttacgaa aagaaagaag ccgaaaacat gacaggggct
    54841 tttgaggcgg ataaatcgga ttattattcg ttggacgatg tacctttcac gatgtatttt
    54901 gacgaagcac gcgcgctgca tggcgcctac tggcgcgctt ggtatggtta cgaacaatcg
    54961 cacggttgtg tgaatatgac catcggtgac gcccattggc tctataactg ggcgcaaatc
    55021 ggggataccg tctatgttca cgatccgtcc ggagagacac cgaccgatcc gtcatattac
    55081 ggcgccggag gagcgtaaaa aattatatgg ctgaggatga ttcctatcgc atcgaacggg
    55141 attctctcgg agaggtgcgc gtacccaaac aggcactcta cggcgcacag actcaacgcg
    55201 ccgtagaaaa cttccgcatc tcgggattga aaccgtggcc ggtttttatt acttcagttg
    55261 tgttgattaa aaagtccgcg gcgattgtca acgccgattt ggggttgctt ccgaccgatt
    55321 atgccgaagc catcgaacag gcggccgatg agattctgaa cggaatgtgg ctggaccaat
    55381 ttgtcgtcga tccgttccaa gccggtgccg gaaccagtca taatatgaac gccaacgaag
    55441 tactcgccaa tcgggcaaac caaattttgg ggaaagaatg catccatccc aacgaccatg
    55501 tcaacatggc tcaatccacc aatgatgtaa ttcccaccgc cattcgcatc ggctgtttat
    55561 tgcgactgaa cgagttaacc gatgcgttgg aaaatttgta tacggcgttg cggcaaaaag
    55621 ccgatgagtt caatactgtc ctaaaatccg gccgaactca ccttcaagat gccgttccgg
    55681 tgacgctcgg acaggaattc ggagcttatg cggatgcgat tctcaaagat accgatcgta
    55741 ttcgccggtc ggcggatact ctggcggaat tgggaatcgg aggaacagca gtaggcagcg
    55801 gtttgaacgc ccatccggat tatcatcgaa aaatgatagc cattctcggt gagctaaccg
    55861 gcataaaatt ccgcacctcc gaaaatttgt ttgaacggat gcagtcgatg gcagatatgg
    55921 ctgacttttc ttcggctatg cgcacgaccg cactctcgct gattcgcatc gcaaacgatt
    55981 ttcgcctctt atcttccgga cccggaacgg gattggatga gttgcatctt cccgctttac
    56041 agcccggatc gagcattatg cccgggaaag tcaacccggt catggcggaa atgttggata
    56101 tggcaatgtt ccatgtcatc ggttgcgata caaccgtcgg gttggcttct caagccggac
    56161 agttggaatt aaatgttatg atgccggtca ttgcccataa cctctttgaa atgatgcaga
    56221 ttatgatcgg cagtatcaat gctttcagcg aaaaatgcgt caaaggatcg caagccaatt
    56281 cggaaaaagc agctcactgg cttgatcaaa atgcgatcat cgtaactgtg ctgaatccgt
    56341 taatcggtta tgcagccggc gcggctttgg taaaagaagc cgttgaaaaa aaggttccga
    56401 ttcgccggtt ggcccatgag aaagccggag ccggactgtt aaaacgcttg gacaacggac
    56461 agccggtctc tcccgccgaa atcgatcacg ctttttcgga cttattgcgg atgacacaag
    56521 gcggattcgt ccggtaaacg accggaaaat agggacaaaa attaatcgat atccatatcg
    56581 gcgaaatcct gatcgatcga gtcgattgaa tcgcctcttt ccaatcgctc agccacttca
    56641 tcaaattcag gtgccatcgg ctccccgctc tgatctttga acgagcgcat cattttaccc
    56701 aattcgcgag gatcatcata cggttcgttg aaactgtcgc tatccgcttc ctgaaccatt
    56761 cggaactggt cactttgtac gacagacacc ggctgaatgg cacgttcgac gaatgtcgat
    56821 cggcaaaatg ggcaaatcac atccatcttt ccgtaatcct catagctgat tcgaatttca
    56881 aatttttttc cgcaattctt acaaaaatag tcataaagcg gcataggatc gtcctctgat
    56941 caattttttc aaaaaaaatt ggttttcatc gcatatcagt catcagaaat aaaacaggat
    57001 tttcatcaaa ttcttctttc aattataatc ttgtaagaaa catgaaaata ttcgacaaaa
    57061 atgtttctga tggttccgga tactctcata tccggaaaaa ttttttccga ttgtcggaaa
    57121 ggtttttctt aatcgagaaa aaaataatgg aaaactatgg aatggttttg ggatgaatta
    57181 gcaaaaaatc atcgggacat tgcggacatg gaattatcac tgccatcatt caaaatctta
    57241 aaatccgatc cgatgattgt gatcatttcc ggtctctccg gatccgggaa agattcggtt
    57301 atcaatgccc ttcgcaataa ttcaaaattg aattttcatt ttgtcgtgac ctgcaatact
    57361 cgaaaacaac gcgatgatga aattgacgga gttcattatc atttcatcag ccgcgaaaag
    57421 ttcttggata tgattcgtaa gaatgagatg attgagtatt cgcctgtata taatgactat
    57481 aaaggggtcc ctaagtttga aatagaaaag ggcattgcca aagggcagga tatgatatta
    57541 cgcttggatc cgcagggcat gaaaaaagtc aaacaggttt atccgaatgc cgtgaccata
    57601 ttcatcgtcc ctcccgatcc cgaaacatgg ttagagaggc tcatcaagcg gggaagcgat
    57661 accgatgaag atctccgagt ccgtattgag actgcgcgcg aggagattaa atcgattccg
    57721 gaattcgatt atatcgttgt caatgaccag ttggataaag cggtcgaaga tgttctaacc
    57781 atcttgcgtg cggagcatca acgaacaacc cgaatcaaag tggagcataa ctgattgtga
    57841 atagactcgc cgtcgattcc gccgcatttc ccgaagagca tcgatttaac tcggacctgt
    57901 tcaactccga acccgaaaaa attccggcgg aaccggatac tctttcggaa gaagacaggc
    57961 aggaggcatc ccgaagagtt cctgttcgta tcccgaatgc aaagccgatc gttacttgga
    58021 ttttatgcgg tgtgaccgtt attatttatc tgatccaagt cggaacccaa caattcatgg
    58081 gtacggatct tcccttggct ttgggggcga agtatggccc tcttattcgc ttagggcaat
    58141 ggtggcgttt gttcacaccc atctttctcc acggttcaat tcttcatatt ttgtttaata
    58201 tgtatgcatt gatcatcctc ggcagagaga tcgaaccggc atacggacat cgggattttt
    58261 tgatatttta tctgatcacc gggtttgcgg gaaacgtttt ttccttcgtt ttttctccgc
    58321 agaccgtttc cgttggggct tccacttcgt tattcggatt gatcgctgcg caggggttcc
    58381 tgatttatca taataagaaa tttatcagga attatcaaag agcaatcaat aacgtgatat
    58441 ttgttgtggt gatcaatttg gttctgggaa tgcaatcagg gattgataat tggggtcatt
    58501 taggcggatt aatttcaggc gtcattattg catttctggc cggtcctgcc tgggatatcc
    58561 gtatcaattc ctcccaaaac ggccttgaat tatatgataa agtcacgcca tcaaagcgtt
    58621 taatagcctt tttattaact tttctgcttt ttgcttcatt ggcttttgcc tttcgatagg
    58681 aacccatatg aattttctac aagctttaat actgggcgtt gtgcaaggat taaccgaatt
    58741 cttaccggta tccagctcgg cacatctggc tttagtcccc tattggttcc aatggaacct
    58801 ttcggaggaa tcggtttttc tgtttggcgt tctcgtccaa atgggcacac tgatttccgt
    58861 aatcatctat ttccggaagg atatcttgca gatactcgtt gccttcttca acggtatttt
    58921 ccgtggaaaa cccttcgaag acccgaaagc ccatttggga tggatgattt tgctggcaac
    58981 cgtccccgca gcggtcgccg gtgcattgat gaaggatacg gtggaatcgg cttttaataa
    59041 tcctcgcgcc atcgccctct ttctgttagt cacggccatt ctgctttttg ccgccgaaaa
    59101 ggtgcctgcc aaagatcgtg atctgcggga aatgaattgg aaggatgccg tatggatcgg
    59161 tatcgctcaa gctgtcgccc ttttaccggg catatccaga tcgggcgcca ccatttccgg
    59221 cggattattt cggaagttta cccgccggga atccagccgt ttcgcttttc tgatctccat
    59281 tccgattatg atcggtgccg gagcactcag tgtattggat ataaccgatt ggaacgcttt
    59341 cagacctctg ttaccggctt tgatctgcgc aacactgacc gctgcggttg tcggatattt
    59401 tgcgatcaag tggatgctga actatatcaa caaaaagccg tttacgattt tttcaattta
    59461 ctgctccgtc atcggaattt ttacttttat cctcacattc atccgctgat tttttctggt
    59521 tgtaccgatt ctccgagtta cattcacaag ttataataaa gaagaattct atggctattt
    59581 tggcttgtga ttgtcctgaa aaatgaaatc atcggaaatt atacgcagac gcagacggaa
    59641 ccggaaaaac aaggttaccc gagctgttaa aagcatttcg gcacttcgca tcctttcttt
    59701 tattctcatt tgcgtcgcaa ttactttcat cattttatgt tgtgccggtt acgtggttat
    59761 tactcagaaa cttccgccga tcgaaaactt tgatgaccat tttgcctcgg caacggaacc
    59821 gacaatcatc tatgaccgca ccggtactta ccctttactc aaattacgct atccgacact
    59881 ggaacatatt tctctcgcaa tcgatgaacc ggataaaccc tgtttttcat cggatttggt
    59941 aaaaattatt gtggcaactc atcaaccgaa tttttggtcc tccgtcggcg cggactggca
    60001 tcgttggagc aatccggacc cggttaccat tgccgaaagg ctggtgcagc aaacattccc
    60061 aaatcaattg ggaagcggag tcgaaaaagc gattttgaca aagatcttgg cgacacaaat
    60121 tacaaaaata tacggacctc aagcaatatt aacctggtat ttgaattcgg cttatttcgg
    60181 acagcttgcg gtgggcgcag acgccgctgc tcggacctat ctggacaagt ccgcttcgga
    60241 tttgaatctt tcggaatcga tcttattaag cgccatcttg gatacgccct ccttgaatcc
    60301 cgttgacgca cccggtgtga tgcgcgaatc ctatttggca cagattgaaa aattggaaga
    60361 aaatgggaca ttgacagaag aagagatccg atcggtccgg agcaccaatt ttgtcatttt
    60421 tgaaccgccg ccgttagaag ctgaaatcgc ctattctccc ggtctgcagc aggcactctc
    60481 ttccgcatat gagacgatcg gtcaggaagc gattgaacgg ggcggatata aaattcgttc
    60541 aaccatcgat tacgatttgc aaactctttt ggaatgcctg accggcatcc atcatccgtc
    60601 aagctcggat gtaacagaac aagaaaaaag ctgcccttat tacccgtttg aaacccggac
    60661 ggaagatcaa acggcggaaa tcgctaaaat tctctcacag aatccaatca gcgctgttgt
    60721 aatcgatatt aaatcagggc agatgatcgc ttcgatcgat gtcggaccgg atggcagcgg
    60781 gaaaagaacg gcgcgcaagg ttttttctca gcatgaaccg ggaagcacat tgacgccctt
    60841 tgttgtctta tccgcattaa atcggggaat ctccctttcc agcatggtct gggatttggc
    60901 tgaacagaga tattatttgc cgtcgggata tcaaaatccc gacggacgtt tacaaggtcc
    60961 gattcgctta cgaaccgcgg ttgacgaaga tatcatggga ccggttgccg aaatgcttcg
    61021 ggaagtcgga accgctcccg tatggaatca agcgactcaa ttcggaatca ccggtgtgaa
    61081 tgccagaacg ccggaaagta ttctcttcaa cggcggttcc gtcaccgtag atatgttggc
    61141 aaatgcttat ctgccttttg cgaaccaggg aagattggtc ggaacgatat acgatgatat
    61201 tcctcgtccg aaaaccattt tatcgattac ctcactcgag atggctgaaa tctctgctca
    61261 aatgcgttcc ggaatttcgt tattggatcc gggcttggcc tacctgatgg taaatctgtt
    61321 agcggaagat aaacaatcct atcgctattt caatcgtcca gcagggacca aagtcggaag
    61381 agtcctcaat tcaaatgatt tttggatgat cggttttacc ccggaaatta tgacagtggt
    61441 ttggctcgga ggaacgccga atggcaccgg aacggatcag gcggcgattt tgctttggca
    61501 gaccatcatg gctgcggcag ttaagaatca accgatcacc ggttggagtc gcccggaaac
    61561 cgtaagccaa ttaatggttt gtaacccttc gggaaaactg cctaccgaca gttgccccga
    61621 tacggtaagt gaagtattct tggcgggaaa tgagccaacg gaatatgatg atctatacgt
    61681 caggatcccc attaatcgaa ataatttaaa aatggccacc gcctatacta aatttgccga
    61741 cattgagcaa aaagtctata tgaatctccc caaggaggca aatgattggg cgattgataa
    61801 ccatatcgaa acaatgccgg cagagtatga cccgatcccg gaaatggacg catctaccgt
    61861 aaaaatttat caaccgataa atttcagcat attcaagaaa accgaaagtc ggaccgatcc
    61921 gttggaaatc aaagccgaga ttaacattcc cgaagaaatt ctctctgttc aggttgtcct
    61981 cgggaagggc ttttatccgg atagctggta cgaattaaac aattcttccg aaattaataa
    62041 cggacgttgg actttggcga aatcggattg gtatgcgttg gatccggctt tatatgattt
    62101 gcgagtttcc gttattacga aagatctgaa atattatcgg gcggatcaat atttcagtgt
    62161 gcaatagatt gaaagcattt tcgataaaat catacaaggt gattaatctt ttttgaaaaa
    62221 aaagagagtt ggtaccctct ttttttgact tcattttgta atttgatcga ttctgttctg
    62281 agccgctttt accggcgtat taaaatctga tcgactgatt ttctgggacg aggattcggt
    62341 atttcagcgg gatagccaag cggcgaaact gccacaaccg tatatg
//
LOCUS       DAOSMM010000005        57702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_592_length_57702_cov_18.862230, whole genome shotgun sequence.
ACCESSION   DAOSMM010000005 DAOSMM010000000
VERSION     DAOSMM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 57702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57702
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_592_length_57702_cov_18.862230"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..417)
                     /locus_tag="PK898_01760"
     CDS             complement(58..417)
                     /locus_tag="PK898_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOU18790.1"
                     /translation="MSILGNIIWFIFGGLWQGLSWLLVGVLWCITIVGIPVGLQCFKI
                     ARLSFFPFGKEIEYHGNTASVLLNILWILFGGLELAIVAFLNGILFCITIIGIPFGKQ
                     CFKLAQLALMPFGSEIR"
     gene            complement(417..1982)
                     /locus_tag="PK898_01765"
     CDS             complement(417..1982)
                     /locus_tag="PK898_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18791.1"
                     /translation="MQINHKKKLPVYIFAIIFLSLLTSATLAYFSPGTFIRGFTAALI
                     FSVPSYLILYFLWKWGGAGKTLAVMMFCSIFLRLGFGLATQNLLPIYGYRDEPQQQGG
                     YLSNDVYRRDEETWLIAQNEKPGFIETITRDYNNDQYGGLAALSVWIYEYLSPDAKRF
                     GLITLLGAFFTAAGIPFLYKAIQQKIPKDEKAYRNLANITAWIYVLYPDSIVFGGSAL
                     REPFLNGILAVAFWALVTLKDHRKKSALVLMICLLACLPLSVFAGGALLCCILLWIWG
                     EFLIPSSKKWLIGGILFIAFSLIVVGLFALPSIQEFVHYDIHSSEINSGWVEKVVGEI
                     GGQFRSVFLAIYGITQPVLPAILFYKPTTPYWKIVGIYRSLGWYIMVPFLFYAIFSAF
                     RIKRKPEKYVFILNALFIIGWIFVSSLRAGGDQWDNPRYREIFLPWLAFFSAWGYCYA
                     RKIKDWWLVRWIVIELIFVGFFSQWYYSRYSHDAIRRFPFWKTVLYIVICSAIVFATG
                     FINPIRKRLKGKE"
     gene            complement(1966..3114)
                     /locus_tag="PK898_01770"
     CDS             complement(1966..3114)
                     /locus_tag="PK898_01770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU18792.1"
                     /translation="MKIAVSSTSTIPSTTANSIQVLMACQGLVQNGQEICLWVPGKES
                     PDFDLLRKQYGLTCQSFPVLPIRSRKRLHRLDFSRQTEKESRKWGADCIYTWTIQVAL
                     IASRHRLPVIFEVHDLPKGTFGKRWFNEFAKSDTVKRFAFITRALEEEVCAEYPNLKK
                     SDCLIAPNGINPEEYQDLPNSHEAKKEMGFEDRIIASCSGHLYPGRGVELFLRLASEF
                     PEIDFYWFGGTADSVKYYREKAAESGLKNVIFTGFIPKSEIPLAQAASDFLLMPYEKT
                     IAGSSGGNSAAICSPMKMFEYLAAGKPILASDLPVIHEILDPTCAVFCEPENLSSWIE
                     GLKMLLDHPEEAAKLAAAAKRKSSFYSWKNREKTILDALTEGKNANKS"
     gene            complement(3168..4559)
                     /locus_tag="PK898_01775"
     CDS             complement(3168..4559)
                     /locus_tag="PK898_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU18793.1"
                     /translation="MIKKIRGWFSDYVFKRVVRNSAYLITSNLISVFLTIYITRLLGV
                     YNYGVLGVITSYVTNVNRFFSFRMNEMIVRYVSEPYEKMDRDQAGALIKFGALIETCT
                     SIFAFLFLLVTAQWGARVFLNDPTLVQPVLIYGITILTGFAMETANGVLRVINRYRAI
                     AIISLVQNILVAVLVLLAAKLNYGLNAILLAYLIGKVILGVVPVILVLIWLPKFIGRG
                     WWKAPISLIRDKKEILRFTLSTNISSTINLFARDGEILWVSAFLSPLYAGYYKTAMTV
                     INMILTPITPFIDTTYPEMMRAIVNNRWNRLKQLIRKVTALSTTWTVGVGLILILFGK
                     QLLYSELNFFGWRFHVFPAEYLPSYQIILILLIGYGIANILFWNRTLLLSLSKAGTAT
                     KISFYGMLLKIIATVLLVPIGPYYLESVILSGYLTGTVLIMTVIGIKGVNHAQRTVVT
                     QNEALSINDMHTG"
     gene            complement(4564..5538)
                     /locus_tag="PK898_01780"
     CDS             complement(4564..5538)
                     /locus_tag="PK898_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU18794.1"
                     /translation="MNHIFVTGAAGFIGARICEMLSEEGIQVTGIDNFSPTYDIALKN
                     YRLNKLQQLPEFSFYPGDITDFDLLTQIFEKNKFDAVINIAGIPGVRLSVENPWLFLE
                     ANTKGALNLLEVCRRNDVGKFIQASTSSVYGKDAPFPTPETADSSHPLQPYSSSKKGA
                     ETMAYAYHYLYGLDVTVTRFFTVYGPAGRPDMAIFRFIKWISEGTPLKVNGDGTQTRG
                     FTYIDDIAQGTILALKKVGYEIVNLGGHESVSINDLIHRLEDIIGKKAIIDYRPIFKA
                     DMLANLADVTKARTLLNWNPRINLDEGLQQTVAWHRQEQSWLQNIKID"
     gene            complement(5541..6839)
                     /locus_tag="PK898_01785"
     CDS             complement(5541..6839)
                     /locus_tag="PK898_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOU18795.1"
                     /translation="MKNKIEKNEARMAVVGLGYVGLPLATVFAEAGYPVTGIDLDPIK
                     TEKVNRGESYIPDIETARIRKLREKKLLQATNDFGVLKDIDLVSICVPTPLRKTGDPD
                     LSFIINAAEKIAPFMHRGMAVAFESSTYPGTTRELVLPKLTADNSLRVGEDFFLIFSP
                     ERIDPGRKDWTTKNTPKIVGGISEKCTEIAVRWYEKALDTIIPVSSPEVAEMAKLLEN
                     TFRMINIGMVNELAMMCNRLNVDVWEVIEAAATKPFGFMKFTPGPGLGGHCIPVDPHY
                     LSWKLKSLHYTARFIDLASEINTNMPRYTVQLIQDALNLQKKSLNGSQVLVLGAAYKP
                     DIDDMRESPSLDVMHLLMQKNAVVHYHDPYIPTLRLDDTVMNSVADLNQALTDADCVA
                     ILTNHSCYKPEVLLEKSRLIVDTRNALGNAGKNNPKVVRL"
     gene            complement(6868..8037)
                     /gene="nagA"
                     /locus_tag="PK898_01790"
     CDS             complement(6868..8037)
                     /gene="nagA"
                     /locus_tag="PK898_01790"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOU18796.1"
                     /translation="MKKILIKNAVIYTPTANGFNGWVIIEDKKILSIQRGSPIPETEQ
                     AMDQVIDAEGKALLPGFIDVHTHGGLGFCTMDADQERLKAIARRNAEHGVTSFLPTTL
                     TDTKEATIRAIEAVTARINVRDGGAKTAGIHLEGPYFNPVKAGAQDPDNIRRADKNEI
                     RDFLKAGKIRLIAMAPEYPENLAAGDLFAAEGITVSAGHTNASYDDLVKAAEHGFSEI
                     THLYNGMGAFGHREPGTIGGALTIPAYTCELICDNVHSHPATHRLAWKAKGPEKIILI
                     TDCIRPSGLPDGTYPFTDKEMVTVSEHGTNLRLKNGSLAGSALTMDRALFNFMNNTGT
                     TLDEIWRCGSLNAAIQIGISAETGSIEKGKLADLILLDAACQVKMTMIEGEIVYQ"
     gene            8168..8239
                     /locus_tag="PK898_01795"
     tRNA            8168..8239
                     /locus_tag="PK898_01795"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8200..8202,aa:Ile,seq:cat)
     gene            8557..9291
                     /locus_tag="PK898_01800"
     CDS             8557..9291
                     /locus_tag="PK898_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU18797.1"
                     /translation="MDIDKLPKYYLLKKELIDRIDREEFKQDEPIPSERELMSQFDVS
                     RITVRRAINELVNEGYLYTIQGKGTYIKNGRYSQNLLSLTSCTQDIIKMGMKPSRRIV
                     ASGIEMAEKKRQHLLHLPEGEKVYFLERVYFADDEPINLTKTWLPYRLVRGIEKFDFS
                     EASLYEVLQNDYHIELTSADRSIEAVLSDEETARLLGITPDIPILLFTCTTYGKVDGI
                     EQPVETFRCHYRSDKFKFYIHQVSEN"
     gene            9405..10556
                     /locus_tag="PK898_01805"
     CDS             9405..10556
                     /locus_tag="PK898_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU18798.1"
                     /translation="MKKKLWVFVSLVLIAMFAMTGAVSASDLKIGIVCSAAGQNDNGY
                     NQSAIEGAKQVSEELGIDYKVIDATSDVPAALETLAEDGYNLVFSLEYDFEALIKGVG
                     GNKPIAEQYPDTTFVVFNDNPNIDENGNVIHKNVISVLFDVHEASFVAGALSVLVNEN
                     NAVLFGEGYHFTDPAKARAIGFIGGTNSNGITVFSYGFIQGINYVAQQLGVKYDYYAK
                     YDAGFSDPATGSTVAGTYYDNGANVVFGCAGNVGDGITSKAKEVGKLAIQVDANKDNQ
                     QPGYVLTSVLKNTKVPVYTISKALKDGTLSSMGNLQTYSLSSGATGITDLSVIGEHIQ
                     KTDAAAAKWAEIKQQVSDISAKIGSDIKVVNAAIGEKFDPASCPNVVIK"
     gene            10666..12234
                     /locus_tag="PK898_01810"
     CDS             10666..12234
                     /locus_tag="PK898_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18799.1"
                     /translation="MSIIRTENLTKKFGDFVANDHINISVEENEIKCIVGENGAGKTT
                     LMNMLYGLLRPTGGKIFIREKEVKINSPLDAISLGLGMVHQHFKLVPSLTVYENIFLG
                     TEIEKKTAKGLRLPFLDEKKEIEEVQKLIDRYKFELNPLDKIEDISVGERQRVEILKM
                     LYRNVDILILDEPTAVLTPQEADDLIASLKELRKQGKTIIEITHKLREVMELSDSVTV
                     MKLGKVIGNVKTSETNEQELAQMMVGRDVVLSVANKHKTGGSQKEIYCVNNLCTINEY
                     GKEVLHNISFSLKTGEILGIAGVEGNGQTELVNVLTGVMESTSGTVIANGNDITNKWP
                     DELRKNGIAIIPEDRFAQGLCRGMDLAQNCIAGYHRRPDVCSRGLLKREVMEEKRDRM
                     IRDFDIRVADPNGLIDQLSGGNAQKVIVAREFESKPQILIACQPTRGVDVGSIEFIHT
                     QLLKFRDAGNAVLLISSELSEIMSLSDRVLVMYKGAIIGEIDPQHTTPEEVGLLMAGI
                     EIKPVSAEQKVKQD"
     gene            12243..13340
                     /locus_tag="PK898_01815"
     CDS             12243..13340
                     /locus_tag="PK898_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18800.1"
                     /translation="MNRKQKWASFGRSLLSSLVPVIAAFIIGGIIISLLGENPFTTYW
                     ILISKSLFTVKGFAYTLHKAAPLILTGLAIAVTFKANIFNMGVEGQLLIGGFFAGIAG
                     ASLTGTSPVVTKVICMAVGMGCGVLFALIPAVLKAYFRVNEMVVTLMLNYAMIKILEF
                     LATGVFRDNGSGYVTTPTIQAGAMFRRLGGSKLTPFFFISLIVFLIMYFVMNRSVLGY
                     EIRAIGLNPVFAEAASMKVRNKIIEIMVISGALSGLAGAGQMMSDQFKYTLTFSGTPG
                     LGWDGMLVSLLGKHDPVGILIAAIFYSALKTGSDKINFYTNVPKEIIAVIQSLIILFL
                     AVQFFDEKTNLFDSLKKKITGRDETTVKEEL"
     gene            13340..14257
                     /locus_tag="PK898_01820"
     CDS             13340..14257
                     /locus_tag="PK898_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18801.1"
                     /translation="MDLLQSILYDMVYHSTPIILCVIGGVFAYKANVLNIALEGMMLC
                     GAFIVMLVDFLTGNLALAVGASILSVLLLGLIFSFFGITCKGNVIVVGLGINMLVSAI
                     AGFVLKVMNSSNILINSLKVSDFKINIPVIENIPILGSLLSGHPLITYLSFLGIFLMW
                     VLMYKTKFGIYVRVVGENEDAARSLGLKVSFYRYMAILIGAFCCALAGINLSLERLGL
                     FTNDMTAGRGFVAIAAIYCGHGDPLVSAVYAIIFGLARSLSINLSIFAGPAAGLFDVI
                     PYVLMTIVLTVVSIYRYKNIKIRGFRINE"
     gene            14250..14816
                     /locus_tag="PK898_01825"
     CDS             14250..14816
                     /locus_tag="PK898_01825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOU18802.1"
                     /translation="MNNTALIIVDMVKDFTDPGGLVYYPQNREILPKIVAVLEESRNF
                     GKLVIFLQHCYRKGKPDKNLRTMRPNCIEGTDGDQIDPMLRVDPIKDYVIRKRRYSGF
                     FGTDLDLVLRENEIKNTVIVGTKTNCCIRATVTDAYNLDYNAIVIRDCVATDSEVVNQ
                     VHLSDIEKYLGRVITADEYIAALKAGEL"
     gene            14813..15778
                     /locus_tag="PK898_01830"
     CDS             14813..15778
                     /locus_tag="PK898_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU18803.1"
                     /translation="MSDDPFDLLVSGYVSMDRIIKINSPARIGYTSLITNRDNAKINY
                     GGCPINICAALNKLGKRAVPVIRVGDDYEEIGFRDFLIQSGIPLDGVELVPNEKTSCC
                     YLVENPQGEHITLFYTGAMDAKYSRPVPDELIKRTKMALITVGAKPDNEYFLEKVQKY
                     SVPLVFSMKSDPDAFPKELLSEILKESLIIFMNESERKSIEAMFGFGSITDLFSFGKA
                     QILVTTCGASGSRYYLPDNGNIRSEMIPACRPDQVADTTGSGDAYLAGFMLGYLDRRP
                     ISDCCRYGSVLASFIIEKEGCCTNIPTMRQFMDRYNRFIREQEAQ"
     gene            15781..16530
                     /locus_tag="PK898_01835"
     CDS             15781..16530
                     /locus_tag="PK898_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU18804.1"
                     /translation="MKTLYMNVDSADIAKNVLFSGDPWRVETVAGYLTDPKRVAFKRE
                     FNTYTGTYKGMPVTVTSTGIGAPSAAIAMEEMYEAGMEVAVRMGTVMSLREGTLGKFI
                     IPYASMRMEKTSATYVPEGYPAVADLDLVQCMNQTVKRMGGEYINGIGCSMDGFYSMM
                     HDSRLSKEMNVDMSVIFEKLKKIRICGVDMESSCILTLGQLMGIKACVLTIVTVLENL
                     KDALEGEERIAAEDLLCRVALEGIYNYRYHV"
     gene            16558..17355
                     /locus_tag="PK898_01840"
     CDS             16558..17355
                     /locus_tag="PK898_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOU18805.1"
                     /translation="MTVWDVFRQKRPFIIGMVHCQPLLGTPGFDGSSQNVLYRAIQDA
                     VTLEKAGVDGIMVENMCDDPLGTLLTLEETVGLTAVATMVKNVVGDIPVGIDAAFCDC
                     KASLAIAKTLGCQFVRIPVFVDTVLYSGGIIYPCAHDCMYYRKQIGAEDVMILADVQV
                     KHTHMLMKDISIEQSAKDAVACGADGLIVTGTATGGETPLEMIRRVKKVVDIPVLAGS
                     GVNSRNIKEQMSVADGAIVGTALKEDGILKNPISFELTSELMKSLRK"
     gene            17371..18525
                     /locus_tag="PK898_01845"
     CDS             17371..18525
                     /locus_tag="PK898_01845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU18806.1"
                     /translation="MMRPMKLAGSELMFGRGCLEYIHKIPEKRVFIVIGGSSMEKNGI
                     LAKVENLFHDNGAETAVFRGVEPDPSFKTVLKGAAEMLKFEPDLIVGLGGGSVMDAGK
                     AMWVCYEHPEIQTLDQLLTANPFPKLRQKARYLCIPTTAGTASEVSRSVVITDDEKGL
                     KHGLGNMEMMPDIAIDDPEVTIFLPPHITAETGMDALTHALEALVSNRANYLSDILAV
                     PAALDIIRTLPIAYANGKDMDAREMMLNASMTAGLAFTNVSLGIVHSLAQTIGGYFHI
                     SHGLADAIILPYIIRYNAAVPYADKKYHDLAAKFGKTDLSEIVDDLNRRLHIPSTLRE
                     PLQEEARFESLIPEMAVTAKNDGCTKTNPIIPDVNEFAELLKRCYYGEEK"
     gene            18529..19245
                     /locus_tag="PK898_01850"
     CDS             18529..19245
                     /locus_tag="PK898_01850"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOU18807.1"
                     /translation="MKLICYLSNGYPSIEESIKMAEIYTHAGADMIEIDFPSRNPYLE
                     SEFIADRMAEALKACDDYDAYMDGMKRVKRLLPSTQFILMVYENTVEEIGPDKFIDFC
                     QSNHYEDLILVGLKDESIKNLCIRNGLKVSCYVQRRMDRMEIEYAKQSNGFVYLQAKA
                     DPDQVNPLYPTLKDCIDHLRCIGIDRPIYCGVGIHTPEDAEAAKEAGADGVFVGSTIL
                     KLHNDIPALIKEINEFKKNC"
     gene            complement(19453..20292)
                     /locus_tag="PK898_01855"
     CDS             complement(19453..20292)
                     /locus_tag="PK898_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU18808.1"
                     /translation="MKEKKSVIFFRYALLTIGVILSCLPMIYMISTSLRPNGALYEYP
                     PRFFPKLQDVTVENYQYIISQNKFYINFFNSVIVSVSTVALAAIISSGLAFVIARFQF
                     PGRKVLFGFIIVTMIIPGTTLIVPQFELAVKLKVVNKLWGLIPFYTAWVIPFSTFMIK
                     GFMENIPDEIIEAATIDGADIFTVFFRIAIPLSTPAIASVSIFNFLTAWEEYPWANTV
                     INDNAIRTLPIAIAGFFGQHQFTQWGYVFAMSALSLIPVILVFILFQKYFVTGLTAGS
                     VKG"
     gene            complement(20289..21191)
                     /locus_tag="PK898_01860"
     CDS             complement(20289..21191)
                     /locus_tag="PK898_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU18809.1"
                     /translation="MKTQSNKNKIQIFLLLLPGLAVFCFFTVYPIIKLFLMSFSKWDF
                     TSMRQQEFIGLQNYREVLADKFFWTSFTNTIVYTLVTVPLQMILGLFVAILVNGIKKF
                     QVTFRVLNYLPVITSWVIASLIFRYVFNTEGLLNYFLSQVLHVTASNIRWLDSRWSGL
                     TVAMILGIWKGIGWNMVIFLAALQQVPIDLYEAAAIDGCNGIQKFFKITLPGIKGTIL
                     FVWVMLTIGGFNVFTSIKMITGGNPAHQTDTILTWMYYKAFSTGKFGYSAAVSFIMAF
                     TLGIMAIFQFKAMRNRDRELEEIG"
     gene            complement(21289..22551)
                     /locus_tag="PK898_01865"
     CDS             complement(21289..22551)
                     /locus_tag="PK898_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU18810.1"
                     /translation="MNEKKKILAIMLAVVLLSLSAIAAGAMSPENQEEITIDFWHHYS
                     AQSAENETLVNVLIPKFESENPGIKVNAVSHEWAELHNKILISAGTGSLPDVARCDIA
                     WLPEFQKMGVLVALDQEMSDFTGVSGKLLESAMSTAKIQDHYYGLGLNTNTKILFYNE
                     KMLKDAGVAVPATMDELISAIKALSGTNADGQQVWGWNEPGLYGWNLCPFIWSFGGSL
                     TNEDQTIATGYINSPATVKAIDTFVSLYKEGALTGFNSGDIPMTDGFGTNRYAMMLEG
                     PWKIAELQGAYPDLAYGTAPMPAGEGGSVSVLGGEDIAMFNTAKKEAAWKFMQFMTGE
                     FAQIEMAKCGQIPVNQSALEDATVKNAAFAPFLEAIKTAKARPTVAAWSEMDNELNVA
                     VTAIINGEKTTQQALDDLAGVWDQLLTQ"
     gene            complement(22553..24355)
                     /locus_tag="PK898_01870"
     CDS             complement(22553..24355)
                     /locus_tag="PK898_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HOU18811.1"
                     /translation="MNQAAIFYRATREFILPVSRQDLFIRLTAARSDIRECRVIFWKR
                     NQTDAASVRFSDMKVAYRDAVHDDFRTVISFPEPVHYIKYYFELIDFAGDIRFLSENG
                     LSDEKPADGFFEFLYVNTADVFSVPEWAKGIIYYQIFPDRFYRSQNAPESSRLVHWNS
                     FPEQDNYFGGDLRGIIEKFDYLKELGAECIYLTPIFKAEFNHKYATTDYFTVDPAFGT
                     LTDLQDLVKLAHSNGIKIILDGVFNHVGRSFPQFSDLLDRGKNSSYKEWFYPSDFPLT
                     SNPLNYECVGDYPYMPKLRTAHPDVRNFILSVMIYWIEQTGIDGWRLDVADEMEMSTL
                     QTIRMVLKEKYPDILLLGETWGDAFRLVGNGDQMDSAMNYLFRNAVLDWIAKGKIRAT
                     DFDRRINQLLSKYNDLTNSVMYNLIGSHDTARFLSEAGENKNKLKLAAAFQMTFCGSP
                     AVYYGDEIGMSGENDPGCRAGMIWDQESQDRDLFQWYRCFIELRKAHPALRKGTFRSN
                     FCDDEKNIYGFIRQTSDEALFVLMNNSENCSEIILPVPDRDAEYRELFSSMIRRPEPL
                     QDGITFYNQEITEYQGQLSIPIDAYSVQIYKKEK"
     gene            complement(24352..25365)
                     /locus_tag="PK898_01875"
     CDS             complement(24352..25365)
                     /locus_tag="PK898_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU18812.1"
                     /translation="MTVTIRDIAKKAGVSPSTVSRVINGKSVISNETRNKIFAVMEEL
                     DFHPNSLARNLANGSSRALALVINAEDKKSFSNQYFTHSVFGIEQIAQTNGYNLIITH
                     NQSKGMTPIEQLIYEKKADGIILPPSLITISLLKKLLNQKFPFVVLGEPPFMRNEINW
                     VDINNAQGSESAVNHLLEKGYRSIVFVGENQQQIFTKNRIAGFRDALEKQGIEPRQDF
                     IIESDGTQEAAYRIAAAFLQKTDPPDAFLCSDNTIAFGILKAVKSLGRAIPQEIGIVT
                     FDNYPLAQYTEPSLTSIDVDTYLLGEHAAFVLLKTIENETNIQHILVGTKLFQRESTD
                     RKL"
     gene            complement(25541..26176)
                     /locus_tag="PK898_01880"
     CDS             complement(25541..26176)
                     /locus_tag="PK898_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18813.1"
                     /translation="MHYEISHPQLLEIRNGTYAETTFGPDQVWYPDRWLRKSGCGPTS
                     ASLLMAYYAFTRPELRSLYIADDLTRHSFIRQMQEMSRFIKPGMMGVDKISKYVDGVI
                     QFANEKGITLSPHVFEVGEKNNGRRNELQGLADFVSKGLSSDSPLGFLVLSRGNETHL
                     QNWHWITVTGTDITEDAILATASDEGKKITFDLSLWYHTTSMNGGLVYFTA"
     gene            complement(26456..26929)
                     /locus_tag="PK898_01885"
     CDS             complement(26456..26929)
                     /locus_tag="PK898_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18814.1"
                     /translation="MKKMIIFSFVSVLMILCFTPVSAFSGSRQIDNETELSDDDYYAK
                     LEQCFSILDNYKEDLDSVYSGEIQTISENWHSIRDNLRWDVGVTCGYIMAAQEPEELS
                     DYAYHIFMGAYHEIAAVEFYIQCISNDNDPKLYDLYQEMLSIAEDFYSYIPLRGH"
     gene            complement(27072..27539)
                     /locus_tag="PK898_01890"
     CDS             complement(27072..27539)
                     /locus_tag="PK898_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18815.1"
                     /translation="MKKIFIFFFALVLAFTAFYPASAQTETEETDVETQSDDNQLYYD
                     SLNQCFALLDLYKDDLDSFSSGKISTLTQKWKDTRNNLRWNSAVSCGYIMSIMEPEEL
                     KDYKSELVYGAYYQLVAIELYILSINNNNDPTLYSLYQQMAEAAQNNYSIIPK"
     gene            complement(27948..29783)
                     /locus_tag="PK898_01895"
     CDS             complement(27948..29783)
                     /locus_tag="PK898_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18816.1"
                     /translation="MLIFSGVIGLLQCVLLPGLILSKVFKIRGGLIERFLYLFPLSLV
                     TNYLVIFLLAALRIYTRPVLSLLIAFEIFALLFLFRRSFRTPLKEILLKAEKTIKQEL
                     QPLTTAFSQPFHNSEEIIQNALWVLSGLGSVSAVLWGIHVWRLNFGTIFSGWDTLFSW
                     NAFAETWATNQIPAFRGSYPQLVAANWSISYVLQGDNPVQFFNTLIPPLFFLWIFLML
                     FDLGFQKKETGFFFAAIIARYMMKKLMGDQIFNGYMDVPAASMVLLSLYAILKGSDRS
                     VKDQQQSLVLGLVFASEAAVTKQSGLIALIVVPIFIHRWLKAAEQQIPQKQKIGWIVV
                     CAGIVLPWYFGGLIQWMMNPDTDTRIVDQGIVQFNQRYEWSHKFYLIRQSLGKYFYVF
                     GISLIGLPFVPKKYRLPFFLVSWPIVILWAMFFTYDTRNLAVALPPLAILCGLAVDGF
                     IQKMLILAERWGAGKIAVGIPFLLITVIGIIAIINLFPDEKLRQDQDNQKKNLFGAGL
                     NQELLYGMLGETHETADILTNYPAYFLPGYGNCCEKVDFKDSVIFEQKLSQSHIRYLL
                     IPQFVENLSWESYDVLNHCTESGKCALLKCSNGYYIPYCLYTVNQ"
     gene            complement(29787..31400)
                     /locus_tag="PK898_01900"
     CDS             complement(29787..31400)
                     /locus_tag="PK898_01900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU18817.1"
                     /translation="MKINNQKAIWGSAVLPAVLGCYLLAISMRRGPGVGGDATIYITA
                     ARNLLAGKGLGLINPAGEFRLLPYFPPFYPLLLAFFGWLGFDPAGFAGPFNMILFAVT
                     VFVVAKTVSQSAEKWIAGLLTGTLLAASPILIPVYSWAMSEPLSIALMAIGFLTLAQY
                     LSTGQRNRLFISAVSCGLSFLTRYSMAACLICACLILLFAQKKQFRIRLSEAVVYGLI
                     AFLPMLIWLIYDIRMTSTVASRSILENVNLWEEIRRFNSQLQPVFLQWLFPDSIIHSP
                     SIPAWGNSIVYYLLLVLPFFICTGIPHRVTSDQNDRILNNLSMIFCSFFYIYLFVILL
                     TSVTTYPPITIGSRMLSPAHFSFLCLIGSIYAKLLRHRQNSSGLKTIITFCLIGLAAT
                     YGLRSIRIARQNAIDGLGYNSVKWRESETVAYIRDHIPPEQPIVTNEETALLFLLNRI
                     TWPMHEVYVNRPDENFYAYESPNPQDIDAGRSAFQRGEAFLVVFDTFEDQMRDIYGQD
                     TQKRIDALFQNLKVVADTDDGVIYTLNRE"
     gene            complement(31387..33165)
                     /locus_tag="PK898_01905"
     CDS             complement(31387..33165)
                     /locus_tag="PK898_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18818.1"
                     /translation="MVSFFRKRTLFFIILWFVLLSVIFTWPLVTHLTHEIAGDIGDNI
                     YFIWMIGWVKKALFQIHANPLDVWFLNYPEGWNLARTEMAPAQLALALPFSLIGGETF
                     GYNMAMLLSFILAGTMMFGWVRHLSGSASAGWVGGTVFACLPYWHSHFLAGHLNLCGI
                     QWIPLFFWGWFDMLRPGTGEFRKKSAILAALGLGLTAFCSQYYVFMLIFVAAIQGILL
                     ILPNRISQLKDMAFWKTFGLFLLIAVSLLILAELPFLTLASSGGMPDRDWGSASLYSA
                     SPTDFLLPATTHFIFGKWVGSHFHRDLWIEATLYAGTISLILYFYGMVRKKKEQNWIW
                     SALTIGIVCAVILAMGTDLHWNEQPVIVSLPRFLQNILQKESARIYLPGLILYKYFPF
                     FGKMRALMRFGLFALVLIACGAGLGAKALIERTGSRSIFISVLLITLIILDFAPRPID
                     RFSEIKPRPVDLWLSERPESGSVMNIPFELNDDQAATYYTLYNNKPFIGGFFNAFPPS
                     QYRSIKEIMDTFPSTESLEKARELKVDFFLFDLEALDSLIGQKPTARWQTLEELKNQC
                     RENGLVELATFDNLSVYGFKEDDENK"
     gene            complement(33158..34165)
                     /locus_tag="PK898_01910"
     CDS             complement(33158..34165)
                     /locus_tag="PK898_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU18819.1"
                     /translation="MRFLIAGFGSIGRRHLRNLRACGQNDLVLLRSNHSTLPTDEIGD
                     IPVETEINKAMAHHPDGVIIANPTAFHLDVAIPAAKAGCSILMEKPITDQIEKIGELE
                     SALKAGNGKLLMGFQFRFHPGLIKIKELMQTGAIGRPLSFQACWSEYLPDWHPWEDYR
                     KSYSARKDLGGGVALTLCHPFDYVRWLFGEVDFIWGYNGKISDLELSVDDVAEVGVKM
                     ENNLVGSIHLDYFRRFVRNDLEITGTQGVLHWNNSDACVTLQNAVYPESVIIPPAEPF
                     ERNQLFLEEMKHFIAVTKREVEPSCTLQDGLITEQLINAVKRSWDEKRIIDFKKEQNL
                     G"
     gene            complement(34227..36737)
                     /locus_tag="PK898_01915"
     CDS             complement(34227..36737)
                     /locus_tag="PK898_01915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU18820.1"
                     /translation="MNKDVKATEHFFMLSSAEGILCFLYLLFLPGGSGEGSLFAFSRV
                     RLALLSAVLLLACFFGIPLVSKNVLNFWIRLSAKKTFRLFALLLSQGILLSGTALLRS
                     LWLLYRTSEDYSWFVVYRRLFPPVCWLGFIAIQAILYFLYRISNDFREVFRQKSHQRK
                     TFLILMGIGIVAAVGIAVTRIGLVKDNAFFGKPTVPLLEWHLILAFLLCLLWIILEMK
                     QIGKAAPVVIKAMPFIVWAVAVGIWLAIPNQHGFFSPPGRAPNFEVYPFSDGSFYGHY
                     ARSLAAGMGFKGRDIPPRPLYIVLLAIFHLLIGNQYDSVILLQTLVLGILPALIYLIG
                     KELHSIGAGLAAALLCILRETNSILSAPFAHNVSTTKYFFADLPTALAAAFFVLMLIR
                     WLKAQREDNYRKNVYAVLTGGALGMMTLIRTQSLSLIFVPLAFLFIRTRKQFSRRLSQ
                     FILFTTALCCCLFPWLIRNKQITGKFVFDHPATQTAEMAISYNIGGTDLTNDEGMNEG
                     EYSEKLTRSILTSVKQYPKEIATFITAHFVNNEICNLRLFPLRDRLSRPMELIKPTTP
                     FWEEINNDQLSIYNLIFLALSYALLAFGISASMEGHMREGLVPLAVILLFNLSTAVGR
                     YSAGRYLIPVDWIIFLYGGIGIAEWLYRSFRLSGFTPPQTSNEETEREAAQFVQPAKL
                     HPKRKLGLYLGIFIAIGLSLPLSERIIPMRFEPASREAVLSKLEVDPNDYDTADAVVM
                     KAIAIYPRYYAAGEGEPESAKQGYGVSDYGRLIFLTLSPGGFGTIELRTETVPAYFPD
                     NSEIWMVGHSQGATTVADLILVQNGDRSYRYTGESKSK"
     gene            complement(36763..37653)
                     /gene="larE"
                     /locus_tag="PK898_01920"
     CDS             complement(36763..37653)
                     /gene="larE"
                     /locus_tag="PK898_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOU18821.1"
                     /translation="MTEKSELASEIQSKLDKLQEILREMGSVIVAFSGGVDSSLLAYL
                     AHETLGDRSLAITVSSELDPPDAIDTAEAFAKKHQIPHEIVKQNLLKVESIASNPSNR
                     CYYCKRQILTEMIRIAKERNFAFVIEGQNIDDLGDYRPGREAIKECGVRSPFVEAGMT
                     KLEIREISKTFQLNTWDQPSTPCLATRIPYGRRIDPKDLIKIGKGENYLKSLGIRECR
                     VRLYDSAAAIEVPERDMKIIADHREQIVRIFNEIGFLHTTLDLAGYRRGSLNQGLEKP
                     IAISASGKQASISSDNKIEN"
     gene            complement(37646..38416)
                     /gene="larB"
                     /locus_tag="PK898_01925"
     CDS             complement(37646..38416)
                     /gene="larB"
                     /locus_tag="PK898_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOU18822.1"
                     /translation="MNDQQIRQIMEAVASGAISVSEGIKALGEFPQESVGNFAVIDHQ
                     REIRTGFPEVIFGPGKTEEQIVEIFERLHDKNPNVLASRISPEVYEHIRERLPYATYD
                     PTARILYTKEKQELLEGITVVTAGTGDIPVAEEAALTAELMGNRVERIYDVGVSGIHR
                     LFNRLPDLRKAKVIIVVAGMEGALASVVGGLVQCPVIAVPTSVGYGSNFHGLAALLSM
                     LNSCANGVGVVNIDNGYGAACLASKINHLRVQKDLQDD"
     gene            38656..39321
                     /locus_tag="PK898_01930"
     CDS             38656..39321
                     /locus_tag="PK898_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18823.1"
                     /translation="MIRKNFFCYLLIPLSVTACVQHLPREIVTSTVPNETSVAQTTAD
                     TAETPVAAEEYALPEGTLVWTDASMTRTLPAGATYADPWDYCQAVGTIDTPGPEYTGT
                     NPPEVIKKEVLRALAISESDGTAHNVVWRCVDGQVFGCDSSMYSTCLTPMNLSTTPPE
                     AAVNECAKAEMDNITLPGAVVGNDTPYEWTCQGGKPVITGQGVTVDEQGYNALIWYPV
                     PRQ"
     gene            39342..40649
                     /locus_tag="PK898_01935"
     CDS             39342..40649
                     /locus_tag="PK898_01935"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOU18824.1"
                     /translation="MNAVDIIIKKRDSKALTDEEIRFFIDGYTEGKIPDYQAAAWAMA
                     VLCRGMTARETTALTRAMAESGEMVSLKGIADIAVDKHSTGGVGDKTTLIVQPIVTAC
                     GLPVAKMSGRGLGFSGGTIDKFESIPGMRLNLSKEEFLNQLKQYGTVLSGQSADLAPA
                     DGKLYALRDVTGTVPSIPLIASSIMSKKIAAGTPLILLDVKVGNGAFMETLEKAEKLA
                     RLMVSIGKKFGRSVKAELTDMNQPLGYAVGNALEVQEAVRMLRGEKSAPDLYEHCIES
                     SALMIKMGRKAKSVDEARHMAEDAIHTGKALESLKLLVRLQGGDVSYIDDLAKFPKAP
                     IIRDLYARSDGWLSEINARMVGEASVLLGAGRVTKADRIDHRVGIIVHHKVGDPIKKG
                     MPLFTVHANNEEKLNQAAELLNNACKYSESEVEKLPQFYGLIE"
     gene            complement(40657..44745)
                     /locus_tag="PK898_01940"
     CDS             complement(40657..44745)
                     /locus_tag="PK898_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18825.1"
                     /translation="MAENILSTQIMQLILNWKLEEATDLLEANQSELSVKDIFELRGL
                     NERYQGYLDNFHEAESIVEEDPDAAQYAVDQIPEDIRITYPGYESLLAKLNTVRDVIA
                     RKQAFERVDEAYNAIVIEFNSAKAAEALETAKQIYPDWDRVPELREKILKTSELQEKL
                     ERGLYLQTEVSALREKGGIASYQKAMSLINEYSSLGLESYGIALFDADHDREDLLKMI
                     TRAEGESWKNRLRPDGIPAETLRLEQSIRSLEDAESKNLRVLYNNNARLMILLTSELQ
                     QEPPDSERAIQINSRINELREKNQKIQTDIHDEVAKRANSYCRLAEKALDAGELSSAE
                     INIRLAKETGKPAAEYDEGDYLGEVSLPTETLDKILSLEERYADALSVRNEIAEKYRI
                     IRDEYNADDNITLNKLLSWVNQIDDYFARDSHTPGLAQFRDELHKRYDSVKSYVFEKG
                     LAEIDRAINKGDPAAAKEKLDQLNSLNLDTDEKRTLRNRQTSINQLEDANNQVENLLK
                     KADEILQNALNPISSDRDSVREYEDIFREIESVYSQNAVKEPQSLIERKESGIRQLQL
                     IESKRTDFESLKAAMDHGLITIESLKTAESFENSESIELKPVKKILAEFWFFCAKSDN
                     NQSNIPHYLAKAEKFAEDSGDANLFEAVSQFRVDFNNKYIEGQRVNFILNSLHNFQND
                     KAYTAGVDYIAKNITAEDRKNPQIQQAADRLEQLYRISQSEFYLRNAKDAFADGNYQD
                     AEEAITKSLDFFYTLEAAQFQKMIISRQQADRALLEEIDDFLSGDIGESGKLTEEQAE
                     EIRYLSEKINQSGKRQIADKKTWAKIAAAQNRIERIINQEKVDFNDLQIQFNNSLILG
                     PQGLNRAADILKQMESRVWVENRSQQILELKLRLDEINNAFRALSGMIEQANSFVRQG
                     DFRSAERILNSFQTGEQDKWPDWLKIQKENAENEIRDLSEKYHLIQSRFTKDSSGNGE
                     VIEKVNRILDPKTSDPALISELSNELNQYKTILEKEIKVDPEKNPYIKQINYLLGLLN
                     WVESSDNTIRGYGIRGSEISLDAIYAIRKNARDFFEQIPAELIGIQPGFAARNKWLEQ
                     RTQAHQLLTQLNESPKKSRQKRSERGQTIKNTIRELERMDLLPREAESLQRYKDTRKK
                     KASRKHRLVGGLISVILILGALYMASPKLIPLLTPSPTLSLTPTLSETPTLTLTNTVT
                     PSPTLTATPTLTMTPTATFTFTPTPVGLTGLINNNRVGVYELPSGMSVMISDGYLEPG
                     TKVNVIRYCEPDFNKGEIWALIFYPSKNRNTGWIRVRLPDSPDFVSLSDINMPAIEVI
                     RANPYLKIECPTLPYQRMPGDEITLTPAVTQTPEEKISTEKP"
     gene            complement(44774..47872)
                     /locus_tag="PK898_01945"
     CDS             complement(44774..47872)
                     /locus_tag="PK898_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18826.1"
                     /translation="MKQDNLEKIITEVNSVRVRDKVIPLVYTGVKNSGGFTSAFEFSN
                     QETEEKYFLKVSEIAEGSKDEISTLTKVNLLFKNEILSEPDAAKRLNYMPVPDVLTDD
                     IDKFNVEFMRMLDRNRFTVQLQTAGKGIPCEKQLPDSLKDRVAIALDFAKILRTCAKN
                     KIAYVDIKPLEHLFWSKTEDKISVTLIDWGNSRQNASSFLLLDDIRKFCLFLPELIYG
                     RKMLEVINQGKYEYPIQKENNPILIRLLGQLSFNTDNPPLNQKYALLIGDMLAGNLTE
                     VRVQNKCVEIWDDILRALNEAVDSLMTETKTTVLSYESLKKEAENFIQKDPESFMDKD
                     FRKAMDPRLISLASYKGWLLPVIRTVQFWYGKVDLIPQQGFDVCIRFVLNDQTTALNS
                     EFQKLAKLISEKISRTKSCPELIPILQDHLKQIQDVIYVWDFLAQSESGQISPEMFQA
                     AFTTSNLRVVDPFLADAYRKQARHISENSVPVSSQNNKVIKPDSLSEETEDSKMENLE
                     DHTTEIKKRTQPLLDVFSRANSFSELRNIGFFADLNELLSGTKGEDSLLVEKELEPVF
                     LKMLDETEDWINKVGPDRFVVPDDQLNSLDWSAVLPQAVADIRVIVKNNPIQIGEFIR
                     SHMFNLQNDLARASSASIELNQKLPVRDLLPRIKALRKKLDTENMEQFKALIENGDFE
                     AAKEIIDLHYIEYPAFYERLRTEMSRQQDEGEDKKALALINSLLNDLSTASGNIETGK
                     YLRNQNNIPYVHQKISSFRQKNMQLYDIQDELARTKNLANESRKASKDLQRLTTLNLV
                     LLVVVIVLSVTLLTVVLIKNFNLDRNVARLEANLSNFQKTYIAAEQATPIPTQPPLPT
                     ATIAITPTEMPIAVAPLITDTEAVPAAQLTEDALLSPADLHLKSLIGKDVVFNLDGNV
                     DLYGDELMTPEKKLGVVINYVQNVPGKLIGYTDKMVNLEIPFNIGKSQISASTMQNVA
                     RQTNIRQYSETPSGTPPVFISQTEIVLTAPATNCGATAESFCNGTISVWIPREKIETS
                     IK"
     gene            complement(47959..48753)
                     /locus_tag="PK898_01950"
     CDS             complement(47959..48753)
                     /locus_tag="PK898_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU18827.1"
                     /translation="MYKKHLVLSDIHENGESLFRFMRKAEAMESGFDDIWLLGDLFGH
                     SNQSNGNAKFDLGFLDALDRLADYPISAVFGNWEYWLLHPERDNTSEAQAKCKAELEE
                     RRQIITERKIRLFDKIMKKEVVCLPEENPQFTVFHGCSFPCHDGQVYHAEPCESYLNP
                     KDLNIVTGGLFENRENLKTQHFLFGHTHIPGYFVYSVSTFVNIWRQFTPSVPEQKIEY
                     GNGIQRFGINPGSAGVEGKDFPRTALILDTEEQTFQFLADREPLYS"
     gene            complement(48746..49918)
                     /locus_tag="PK898_01955"
     CDS             complement(48746..49918)
                     /locus_tag="PK898_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU18828.1"
                     /translation="MDNSIDLKQKFLLDPAIHFLNHGSFGACPKEVIETFFSWTLRME
                     KQPVLFLGREYHELLKEARIILANYLNTSADSVVYIPNATYGVNIMARSINLKPGDEV
                     VTGNQEYGACENVWIDCCQKSGAILKKADIRLPYGSDQELIDCLWEQVTPRTRVIYLS
                     HLTSPTALTFPIREICRKAREEGIITCIDGAHAPGQLPVDLLSLECDFYTGNCHKWMC
                     APKGSAFLYVSEQWIQKVAPLIISWGHGPFLENAVGNPLIDFNQWTGTNSPCAALSVP
                     AAIRFMEANHWDQVRRACHLLLFETLREIGQITQLPLIYPNDDAYAQLAVTEIPYLPD
                     PLVLKNFLYDRYQVEVPIISWNGRTFVRISVQGYNTKMDVEALVEGLGDFLAHKHV"
     gene            50372..50920
                     /locus_tag="PK898_01960"
     CDS             50372..50920
                     /locus_tag="PK898_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HOU18829.1"
                     /translation="MRFYRFLIVCILGFTFLITGCESAQSTLPTETLIREEIEPLLLP
                     ESMREPIEDSEKGILYWQKTLYLSDHPEKYIGEPVEVLGFIYRREEDPKEVFFVGRLL
                     LSCCYDDALPQGLGVYLPDNEKWENDQWVTVKGHWDRIRFGENSRYLIIPESISEAEP
                     PEDPYIYDKTEPAETNSANENI"
     gene            complement(50992..51480)
                     /locus_tag="PK898_01965"
     CDS             complement(50992..51480)
                     /locus_tag="PK898_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOU18830.1"
                     /translation="MLSKQMQQALVEQINKEFHSAYIYLGMEAYFENENYKGMSAWMN
                     KQVKEEISHAMKFFRYVYERSGEVSLDTIEKVSVDYKNPLDVFKAGLEHEKFITSSIH
                     GLYDLALEEKDYGTQELLRWYISEQVEEEANFEYIIAMLEKSGDNPGGLMILDEKLGA
                     RE"
     gene            complement(51654..53504)
                     /locus_tag="PK898_01970"
     CDS             complement(51654..53504)
                     /locus_tag="PK898_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU18831.1"
                     /translation="MRPIPYGLWKSPISEQAVAQRGRINTLKWGSDNRLYFSGNRGGN
                     SFLYEYRCDSGLKKLNGDLPVGGNIGYGGGDFDVRNEKVLFSAGKRGLFLQNLNKPAF
                     KLLTNDRYMTASPVISPNGRFVAYISGDGTDDQMALLRLDERSWPFLWVHGADFYMQP
                     AWSPRGDYFTWVEWDHPDMPWDGSKVMIGKVDPENGTISEIVHIAGKMPFPASQPKFS
                     PDGKMLSYIRSNGEWEDLILRNLESGEEKILVHGDHFALTVPAFAQGVQTYDWFPDSR
                     QIAFLQIFGTRSKICIVSVESGEIRILNSPQYSSFDVISVSPETGMIAAVADSPLQPT
                     QIILVNDDGFKIVFRLSDDLIDEEYISKPREISWEAPDGTPVYGIFYPPTNPDAHWVG
                     LPPAFVHIHGGPTGKADLRFNPEIQFFTSRGFGYLEVNYRGSCGYGTGYLKSLYGNWG
                     VYDVEDAVLGASCLSALHLADRNRLLIIGGSAGGFTVLNTLIQYPDSFKAAACLYGVT
                     DLFGLAINTHKLELHYTDSLVGPLPEAAPLYEERSPIRHVEKIKTPLAIFQGDADPVV
                     IPSQSNELAARLKCPHVYRLYKEEGHGFRRPETITDYLSTLYSFATQYLL"
     gene            complement(53840..54226)
                     /gene="rplL"
                     /locus_tag="PK898_01975"
     CDS             complement(53840..54226)
                     /gene="rplL"
                     /locus_tag="PK898_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOU18832.1"
                     /translation="MADIEKLVEALSSLSVLEASELVKALEDKWGVSAAAPVATVAAA
                     PVAEAAAPAEEATEFDVIIKDAGPKKIETIKVIRVLTNLGLVEAKQMAETAGAKVLSA
                     VGKEAANDAKSKLEAAGATVEVKPAS"
     gene            complement(54276..54803)
                     /gene="rplJ"
                     /locus_tag="PK898_01980"
     CDS             complement(54276..54803)
                     /gene="rplJ"
                     /locus_tag="PK898_01980"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOU18833.1"
                     /translation="MAFTKSEKQKMMEQYSQWLQKSQGVFMLEYSKMDMPAIDEIRAK
                     AREAGGEVHVVKDTLIQKVLEKENYKLQKDITGTSLVGFAFTDAAAMAKVFSDLVKNE
                     AFKIKGGFLDKNEISESQVKALASLPPLPVMRAKLLGTILAPASQLTRVIAEPGRSLA
                     AVIQAHISKEEPTAA"
     misc_feature    complement(54828..54963)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(55002..55721)
                     /gene="rplA"
                     /locus_tag="PK898_01985"
     CDS             complement(55002..55721)
                     /gene="rplA"
                     /locus_tag="PK898_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOU18834.1"
                     /translation="MAKHGKKFQEAQKKVNPSTNYAPKDAIDLVKETSITKFDATVEI
                     HLRTGLDPRQADQQVRDVVVLPNGLGKTVRVLVFAQGEGAAKAEEGGADIIADSDEWI
                     QKIQQGFTDFDVAIATPDMMAKAGRLGRVLGPRGLMPNPKAGTVVPAEDLPRVIKEAK
                     AGRVEFRLDKSGNIHAPIGKVSFDQKALYENLAAFLDAVRKARPAGAKGTYIKKITLA
                     STMGPGIHVDLSAAQGLEPIA"
     gene            complement(55773..56198)
                     /gene="rplK"
                     /locus_tag="PK898_01990"
     CDS             complement(55773..56198)
                     /gene="rplK"
                     /locus_tag="PK898_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOU18835.1"
                     /translation="MAKKLKAIVKLNLNAGKANPAPPVGPALAGHGINIMAFVKEYNA
                     RTQTRIGEIVPAEISVYTDGSFTFVLKTPPASYMIKRAAGIETASPEGSKGRVGKITR
                     AQVREIAETKLKDMNAVDIEGAMKQIEGTARNMGIQVVD"
     gene            complement(56299..56835)
                     /gene="nusG"
                     /locus_tag="PK898_01995"
     CDS             complement(56299..56835)
                     /gene="nusG"
                     /locus_tag="PK898_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOU18836.1"
                     /translation="MESNEERNWYVIHCYSGYENKVRHNLEQRIDSMGMKDKIFDVVI
                     PTQEEIEVKEGKRKTIERHIFPGYVLVNMILTEESWFIVRNTPGVTGFVGMGNQPTPL
                     RPEEVNSIIKRMEAEAPVVSVTYKVGEKVRIIDGPFNDFRGTVAEIDQDKSKVRVMVN
                     FFGRETPVELDFLQVEKA"
     gene            complement(57005..57247)
                     /gene="secE"
                     /locus_tag="PK898_02000"
     CDS             complement(57005..57247)
                     /gene="secE"
                     /locus_tag="PK898_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOU18837.1"
                     /translation="MADTKIKKTEKPAKPSAPKKPNKIAKWWNETIGELRKVTWPTRE
                     EALHMTKIVLIVVFAMAVFLGIVDFLFSELIGTIVG"
     gene            complement(57319..57392)
                     /locus_tag="PK898_02005"
     tRNA            complement(57319..57392)
                     /locus_tag="PK898_02005"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57356..57358),aa:Trp,seq:cca)
     gene            complement(57439..57609)
                     /gene="rpmG"
                     /locus_tag="PK898_02010"
     CDS             complement(57439..57609)
                     /gene="rpmG"
                     /locus_tag="PK898_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOU18838.1"
                     /translation="MASKKKDVRPVITFACTECKERNYTSQKNRRNDPGRMELMKYCS
                     RCRKHTLHREAK"
BASE COUNT        15861 a        12751 c        12916 g        16174 t
ORIGIN      
        1 attcgacagc ttcaattttg cgcttcaggc attcgaatca tccggatttc cgttttctta
       61 tcggatttcg gatccgaagg gcatcagtgc caattgggct aatttgaagc attgttttcc
      121 gaagggaatc ccgataatag tgatacaaaa aagaatgccg ttcaggaatg cgacgattgc
      181 caattcgagc ccgccgaata agatccagag aatattaagt aaaacagagg ctgtgtttcc
      241 atggtattca atttcttttc cgaacgggaa aaaggacaat cgggctattt tgaaacattg
      301 caacccgacc ggaatcccaa cgatggtgat acaccataaa actccgacca gaagccagga
      361 cagaccctgc cataatccgc cgaaaataaa ccaaataata tttcccaaaa tactcattat
      421 tcttttcctt ttaatctttt gcgtatagga ttaataaacc ctgtcgcgaa tacgattgcg
      481 ctgcagatga caatatagag cacggttttc cagaagggga aacgtctgat cgcatcatgc
      541 gaatagcgac tgtaatacca ttgtgagaaa aatcccacaa aaatcagttc gataacgatc
      601 caccgcacta accaccaatc ttttattttt cgggcatagc aatatcccca ggcactgaag
      661 aaagcaagcc aagggaggaa aatttcccga tagcgtggat tgtcccattg atcccctccc
      721 gcacgaagcg agctgacgaa aatccagccg attataaaca gcgcattcaa gatgaatacg
      781 tatttctccg gttttcgttt tatccgaaag gcagaaaaaa tcgcatagaa aagaaacggc
      841 accattatat accaacccaa tgatcgataa attccgacaa ttttccaata aggggtggta
      901 ggtttataga ataaaatggc cggtaaaacc ggctgggtga tgccgtaaat tgccaaaaaa
      961 acggaacgga attggccacc gatttctccg acaacttttt caacccagcc ggaattgatt
     1021 tcggaactgt ggatatcgta atggacaaat tcctgtatgg agggaagggc aaataaccct
     1081 acgactatca gagagaatgc aatgaaaaga attccgccga tcagccattt tttggaagat
     1141 gggatcaaaa attcgcccca tatccagaga agaatgcagc ataataaggc accgcctgcg
     1201 aaaacagata agggcagaca agccaacaga caaatcatca aaacgagcgc ggattttttt
     1261 cgatgatctt tcaatgtgac taatgcccag aaggctactg caaggattcc gtttaagaac
     1321 ggttcgcgca gagctgaccc gccgaataca atactgtcgg gatacaagac atagatccag
     1381 gcggtgatat ttgccaggtt gcgataagcc ttctcatctt tcggaatttt ttgttgaatc
     1441 gctttgtata ggaaagggat accggctgcg gtaaaaaaag cacctaataa agtaattaat
     1501 ccaaatcgct tcgcatccgg agaaaggtat tcataaatcc agacagaaag agcagccagc
     1561 ccgccgtact gatcgttgtt atagtctcgg gtgatcgtct cgataaaacc gggcttttca
     1621 ttttgtgcaa tcagccaggt ctcctcatcc cgtcgataaa catcattcga aagataacca
     1681 ccctgttgtt gcggttcatc ccggtaaccg tatatcggta acaaattttg ggttgccaag
     1741 ccgaatccaa ggcgaagaaa aatggaacaa aacatcatga cggctaatgt tttccctgcg
     1801 cctccccatt tccataaaaa ataaagaatc aaataggaag ggacggaaaa aatcagcgca
     1861 gcggtaaacc cgcggatgaa tgttcccggg ctgaaatagg ctaatgtagc tgaggtaaga
     1921 agtgaaagaa aaatgattgc gaagatatat accggtagtt tctttttatg atttatttgc
     1981 attttttcct tccgttaacg cgtcaagaat cgttttttct ctgtttttcc aactatagaa
     2041 cgaagatttc cgctttgccg cagcggcgag ttttgctgct tcttcgggat ggtcgaggag
     2101 cattttcaac ccttcaatcc aagaagagag gttttccggc tcgcaaaata cggcacaagt
     2161 cgggtccaaa atttcgtgaa tgaccggaag atcggaagcc aggatcggtt taccggcagc
     2221 taaatattca aacattttca ttgggctgca aatggcggcg gaattcccgc cgctgcttcc
     2281 ggcgattgtt ttttcgtaag gcatcaataa aaaatcactt gctgcctgtg ccagcgggat
     2341 ttcggattta ggaataaaac cggtgaagat tacatttttc aatcccgatt cggctgcttt
     2401 ttcccgatag tatttcacgg aatccgccgt tccgccgaac cagtaaaaat cgatttcggg
     2461 aaattcgctg gcaaggcgaa gaaaaagctc cacgcccctt cccgggtaca aatgaccgct
     2521 gcaggaagcg atgattcgat cttcgaatcc catttctttt ttcgcttcat gactatttgg
     2581 aagatcttga tactcttccg gattaatacc gttgggtgcg atcagacaat ccgatttctt
     2641 taaattggga tattcggcac agacttcttc ctccaacgcc cgtgtgataa aagcgaagcg
     2701 tttcaccgta tcggattttg caaattcatt gaaccatcgt ttaccgaaag tgcctttcgg
     2761 caaatcgtgc acttcgaaaa tgacgggcaa ccgatgccgg gaggcaataa gcgcaacctg
     2821 gatagtccag gtatagatgc aatctgctcc ccacttgcga gattcttttt ccgtctgccg
     2881 gctgaaatcc aatcggtgca acctttttct tgatcggatg ggtagaacag ggaagctttg
     2941 acaagttaat ccatactgtt tccgtaacag gtcaaaatcg ggtgattctt tcccgggaac
     3001 ccataggcag atctcctgcc cgttttgaac caatccctga cacgccatta aaacttggat
     3061 ggaattggcg gttgtggagg ggatggttga ggtggaactg acggcgatct tcatacactc
     3121 attttactct ggttcaatat tcggaaagat tacggaaccg gtaccgatca accggtatgc
     3181 atatcattta tgctcaaggc ttcgttttgc gtgacaacgg tcctttgggc atgattaacc
     3241 cctttgatgc cgataaccgt catgatcaaa accgttccgg tcagatatcc ggaaagaata
     3301 accgattcca aataataagg acctatggga acgagaagaa cggtcgcgat gatttttaat
     3361 aacataccgt aaaaactgat tttcgttgcc gttcctgctt tacttagaga aagtaaaagc
     3421 gttcgattcc aaaaaagaat gtttgctata ccgtatccga tcagcaaaat caggatgatt
     3481 tgataggatg gcagatattc ggcggggaaa acatgaaacc gccaaccgaa aaagttcaat
     3541 tcgctgtaga gtaactgttt cccgaacagg attaatatca gaccgactcc gaccgtccat
     3601 gtcgttgaga gggctgtcac ctttcgaatc agttgtttca ggcgattcca tcgattattg
     3661 acgatcgctc gcatcatttc tgggtaggtt gtatcgataa acggtgtgat gggtgtcagt
     3721 atcatattga ttaccgtcat cgccgtttta tagtatccgg catacagcgg gctcaagaag
     3781 gcactgaccc ataatatctc cccgtctctt gcgaaaaggt ttatggtact gctgatattg
     3841 gtggaaaggg taaaacgcag gatttctttt ttatcccgaa tcaggcttat cggagctttc
     3901 caccagcctc ttccgataaa tttcggcagc cagatcagca ctaatatgac cgggacaacc
     3961 ccgaggatta cttttccgat caaatacgca agtaggatgg catttaaacc gtaatttaat
     4021 tttgcggcta aaagaaccag aacggctacc agaatatttt gaaccaagct gataatcgct
     4081 atcgcccggt agcgattgat gacccgtaaa acaccgttgg ctgtctccat cgcaaaaccg
     4141 gtcaagatgg taattccgta aataaggacc ggttggacta atgtgggatc atttagaaaa
     4201 acacgggcac cccattgcgc tgtcacaagc agaaaaagaa atgcgaatat cgaagtacaa
     4261 gtttcaatca aagcgccgaa tttgatcaat gctcccgctt gatcccgatc cattttttcg
     4321 tatggttcgc tgacatagcg aacaatcatt tcgttcatcc ggaaagagaa aaaccgattg
     4381 acattggtta cataactggt aatcactcca aggacaccat aattgtagac acccaacaat
     4441 cgggtgatat agattgtcag aaaaacgctg atcaggttgc tggtaatcag gtaagcagag
     4501 ttccgcacaa ctcgtttaaa aacgtaatcc gaaaaccatc cccgtatttt tttgatcata
     4561 aagtcaatcg attttgatat tttgcaacca gctttgttcc tgccggtgcc aggcgaccgt
     4621 ttgttgcaat ccttcatcca gattgattcg cggattccaa ttcagcaatg tccgagcttt
     4681 cgtcacatct gccagattgg caagcatgtc cgctttgaag atcggtcgat aatcgatgat
     4741 tgctttcttt ccgattatgt cttccaaccg atgaatcaaa tcgttgatcg aaactgattc
     4801 atgcccaccc agatttacga tttcgtatcc gacttttttt aatgccaaaa ttgttccttg
     4861 agcaatatca tcgatataag taaagccgcg ggtttgggta ccgtctccgt taactttaag
     4921 aggtgttcct tccgatatcc acttgataaa tcggaaaata gccatgtccg gacgtcccgc
     4981 aggcccatag acggtaaaga agcgtgtgac ggtaacatcc agtccgtaaa gataatggta
     5041 agcataagcc atggtttccg cccctttctt gctggaagaa tacggttgga gcggatggct
     5101 tgaatcggct gtttcgggtg tcgggaaagg tgcatccttt ccgtagacac tggatgtgga
     5161 agcttgaata aattttccta catcattgcg gcggcagact tcaaggagat ttaaagcacc
     5221 tttggtattc gcttccaaaa acaaccatgg attttcgaca ctcagccgca ctcccggaat
     5281 cccggcaata ttaataaccg catcaaattt attcttttcg aaaatctgtg tcagtaagtc
     5341 aaaatctgtg atatccccgg gataaaagga aaattcaggc agctgttgaa gtttgttcag
     5401 acgatagttc tttaacgcaa tatcataagt cggactgaaa ttatcgatac cggtaacttg
     5461 aatgccctct tcgctcaaca tttcgcaaat tcgcgcaccg ataaatcccg cggcgcctgt
     5521 cacgaaaata tggttcattt ttataacctg acaactttgg ggttattttt tccggcattt
     5581 ccgagcgcgt tgcgggtatc gacaatcaac cggcttttct ccagtaagac ttccggttta
     5641 taacaggagt gattggtgag aatcgcaaca caatccgcat ccgtcagagc ttgattcaga
     5701 tccgctacgc tattcatgac agtatcatcc aatctcaatg tcgggatata aggatcgtga
     5761 taatgaacga cggcgttttt ctgcatgaga agatgcatta catccaaaga cgggctttcc
     5821 cgcatatcgt caatgtccgg tttgtaagcc gctcccaaaa ccaatacttg agagccgttt
     5881 aagctcttct tctgcaaatt taatgcatcc tggatgagtt gaacggtata acgcggcata
     5941 ttggtgttga tttccgaggc aagatcgata aaccgcgcgg tgtaatggag agatttcagc
     6001 ttccaagaaa ggtaatgcgg gtctaccggg atacaatgtc cccccaagcc gggtccggga
     6061 gtaaatttca taaatccgaa cggttttgtt gcggcggctt caatcacttc ccagacatcg
     6121 acgttgagcc ggttacacat catagccaat tcgttgacca ttccgatatt gatcatccga
     6181 aatgtgtttt caagcaactt tgccatttcc gccacttccg gagaggatac gggtatgatc
     6241 gtatctaacg ctttttcata ccaacggacc gcgatttccg tgcatttttc actgattccc
     6301 ccgacgattt tgggagtgtt tttcgttgtc caatccttcc gcccgggatc gattcgttcc
     6361 ggtgaaaaaa tcaggaaaaa gtcttccccc acccgcaggg aattgtcagc cgtcagtttc
     6421 ggtaagacca gttcgcgggt ggtacccggg taggtactgg attcaaacgc gacggccatt
     6481 ccgcgatgca taaacggggc gattttttcg gcggcgttga taataaaaga taaatccggg
     6541 tcccctgttt ttcgcaaggg agttgggacg caaatgctga cgagatcgat gtctttcaat
     6601 acaccgaaat catttgtggc ttgaagcagc ttcttttccc gcagtttccg aattctggct
     6661 gtctcgatat ccggaatata gctttcgccc cgattaacct tctctgtttt gatcggatcc
     6721 aaatcgatac cggttacggg ataaccggct tccgcaaaaa cggttgccaa aggcagcccg
     6781 acatacccca gccctaccac agccattcga gcttcgtttt tttcgatctt attcttcaat
     6841 atttcaaaag agtcttccat aaaatgccta ttgataaacg atttcccctt caatcattgt
     6901 catttttacc tgacaagcag catccaataa aatcaaatcc gctaattttc ctttttcgat
     6961 actgccggtt tccgcggaaa taccgatctg gatggctgcg tttaaactgc cgcaacgcca
     7021 gatttcatcc agcgtggtac cggtattatt cataaaattg aaaagggcac gatccattgt
     7081 cagagcgctg cctgccagac tgccgttttt cagccgtaaa ttggtcccat gttcggaaac
     7141 cgtcaccatt tctttatccg tgaaaggata agttccgtcc gggaggccgg acggtcgaat
     7201 gcagtcggta atcaggatga ttttttccgg accttttgct ttccaagcga ggcggtgggt
     7261 tgcgggatgg gaatggacat tgtcacaaat caattcacag gtatacgcgg gaattgtcag
     7321 ggctccgccg attgtcccgg gctcacggtg tccgaacgca cccataccgt tgtataaatg
     7381 tgtaatttcc gaaaatccat gctccgccgc tttaaccaga tcatcataag aagcatttgt
     7441 atgaccggcg gatacggtga tgccttccgc ggcaaacaaa tcacccgcgg caaggttttc
     7501 ggggtattcc ggtgccatgg caatcaatcg gattttgccc gctttaagga agtcgcgaat
     7561 ttcattttta tccgcacgcc gaatattatc cggatcttgc gcacccgctt ttacggggtt
     7621 aaaatacggt ccttccagat ggattcccgc ggttttcgcc ccaccgtcac ggacattgat
     7681 tcgagcggtg acagcttcga ttgcacggat tgtggcctct ttcgtatcgg tcagggtggt
     7741 tggcaggaag ctggtcacgc cgtgctccgc attgcgacgt gcgatcgctt tcagtcgttc
     7801 ctgatcggca tccatcgtac agaaacctaa accgccgtgg gtatgcacat cgataaagcc
     7861 gggcaacaat gctttccctt ccgcatcgat cacttgatcc atggcttgtt cggtttccgg
     7921 aatgggactg cctctttgaa tgcttaaaat tttcttgtct tcgataatta cccaaccgtt
     7981 aaacccgttc gcggtcggag tataaatcac agcatttttg atcaatattt ttttcataaa
     8041 aacctcccgc aatttctgcg gataacctta ttcctatgat tcatctgttt tggaatgata
     8101 atctccacga aatgacaggt gattattcca atttgatcga acttctatta taatatggaa
     8161 agttgcaggg cccatagctc agcggcagag cagggggctc ataacctctt ggtcgtaggt
     8221 tcgaatccta ctgggcccac tgattcctaa tttgataaat aatttcacag attacaaaat
     8281 tgataactcg gttagatgat gccgatgatc ggctgtatcg gttattaaat acgctaccca
     8341 gtcggtttag atttgcgtaa aaggatggct gccgtacgaa tctgatcttg aagaaatcat
     8401 ctcgcctcag ttgatatatt tttagaagga atctgaaaaa ttgtcataaa aaactagtga
     8461 tgtttttcac tgacaggctt tctcggattc gatataatca tcttaactgg ttataacaac
     8521 ataataacat aatgagataa cgagtgaaag gttttcatgg atatcgataa acttccaaaa
     8581 tactatctgc tcaaaaaaga actgatcgac cgtatcgatc gggaagaatt taaacaagac
     8641 gaaccgatcc ccagcgagcg cgaattgatg tcacaattcg atgtgagtcg gattaccgtt
     8701 cgccgggcaa tcaatgagtt ggtaaacgaa ggctaccttt ataccatcca agggaaaggg
     8761 acctatatca agaacggtcg atattctcaa aatttgctct cgctgaccag ctgtactcag
     8821 gacattatta agatgggaat gaagcccagc cggcgaattg tcgccagcgg tatcgagatg
     8881 gccgagaaaa aacgccaaca tcttctgcat cttccggaag gtgagaaggt ctattttctt
     8941 gaaagggttt attttgcgga tgatgaaccg atcaacctga ccaaaacttg gctgccgtat
     9001 cgtttggttc gcggaataga aaaattcgat ttttctgagg catcactgta tgaagtttta
     9061 caaaatgatt atcacattga actgacaagc gccgatcgga gtatcgaagc ggttctttcg
     9121 gatgaggaaa cggcacggtt gttgggaatt acgccggata ttccgattct gttgtttacc
     9181 tgtactacct acggtaaagt ggatgggatc gagcagccgg tggagacttt ccgttgccat
     9241 tatcgcagcg ataagttcaa attttatatt catcaggtat cggaaaattg atgtgaattc
     9301 ggagatttct tgaagggttt tgaataaagt cggaaaaaat atgatggctt ggacaaaaga
     9361 ggtgaataag caatccatca tcattttatt ataaggagat tggcatgaag aaaaaattgt
     9421 gggtatttgt gtctttggtt ctcatcgcta tgtttgcgat gacgggtgcg gtttccgcat
     9481 cggatctgaa aataggaatt gtctgttctg cggcaggcca gaatgacaac ggttataacc
     9541 agtctgcgat tgaaggtgcg aaacaggtca gtgaagaatt gggcattgat tataaagtga
     9601 tcgatgcaac cagtgatgta ccggcggctc tggaaacatt ggccgaagac ggctataatc
     9661 tggttttttc tctggaatat gattttgaag ccttgattaa aggcgtcggc ggcaacaagc
     9721 ctatcgccga acaatatccg gataccactt tcgttgtttt caacgacaac ccgaatattg
     9781 atgaaaatgg gaatgtcatt cataaaaatg tcatttccgt cctattcgat gtccatgaag
     9841 cttcctttgt tgcaggtgca ttgagtgttt tagtcaacga aaataatgct gttttgttcg
     9901 gagaagggta tcattttacc gatcctgcca aagcgcgtgc cattggcttt atcggtggaa
     9961 ccaattcgaa cggaattacc gttttctcct atggttttat ccaaggcatc aattatgtgg
    10021 ctcaacaatt gggtgtgaaa tacgattatt atgccaaata tgatgccgga ttcagtgatc
    10081 ccgcgaccgg cagtacggta gccggtacct attatgacaa cggtgcgaat gttgttttcg
    10141 gatgcgccgg taatgtcggt gacggtatta cttcaaaagc caaggaagtc ggaaaattgg
    10201 cgatccaggt ggatgcaaac aaggataatc agcagccggg ttatgttctg accagtgttc
    10261 tgaaaaatac aaaagtgccg gtttatacca tttcgaaagc tctgaaggac ggaacgcttt
    10321 caagtatggg aaatttgcag acctattctt tgagctccgg cgcgacgggt attacggact
    10381 tgtcagtaat cggcgaacat attcaaaaga cggatgctgc cgcagctaaa tgggctgaga
    10441 ttaaacagca ggtcagtgac atttccgcaa agatcggcag cgatatcaag gttgtcaatg
    10501 cggcgatcgg cgaaaagttt gatcccgctt cctgcccgaa tgttgtgatc aaataattgt
    10561 tcctccgaag gaagatgatg aaacaaaaga gtgcccaaag atctttgggc acccttttgg
    10621 aaagaaaatc gtcccataag atccatatga atacaaagtg aaagaatgag cataattcga
    10681 accgaaaatt tgacaaaaaa atttggcgat tttgtcgcga atgatcatat caatatctcc
    10741 gtagaagaaa atgaaatcaa gtgtatcgtc ggagaaaacg gagccgggaa aaccaccctg
    10801 atgaatatgc tttacggatt gttacgacct accggcggca aaatatttat tcgtgaaaag
    10861 gaagtcaaaa taaacagtcc tttggatgcg atatccttgg ggctcgggat ggttcaccag
    10921 catttcaaac tggtcccaag cttgacagtt tatgaaaata tttttttggg aacagaaata
    10981 gaaaaaaaga cggcaaaagg tctacgctta ccttttttgg atgaaaaaaa ggaaatagag
    11041 gaagttcaaa aactcattga tcgatataaa tttgaactga atcctcttga caaaatcgaa
    11101 gatatctccg ttggcgaaag acaacgggtt gaaattttga agatgcttta tcgaaacgta
    11161 gatattctta ttctggacga accgacagcc gttctgacgc cgcaagaggc tgatgatctg
    11221 attgccagtt taaaagaatt acggaaacaa gggaaaacaa tcattgaaat tacacacaaa
    11281 cttcgtgaag taatggagct ttcagacagt gtgacggtga tgaaactcgg taaagtgatc
    11341 ggtaacgtaa aaacttccga aacaaatgag caggaacttg ctcagatgat ggtgggtcgt
    11401 gatgtggttc tttcggtagc aaacaagcat aaaaccggcg gcagtcagaa agagatctat
    11461 tgcgtaaaca atctttgcac cataaatgaa tatggcaagg aagtgcttca taatatcagt
    11521 ttctccttga aaacaggaga aattctcggc attgccggtg tggagggaaa cggccagacc
    11581 gaattggtga atgtattgac gggtgtgatg gaaagtactt caggtactgt tatagcaaac
    11641 ggtaatgaca ttaccaataa atggcccgat gagttgcgaa agaacgggat tgccattatt
    11701 ccggaagatc gttttgctca aggactttgt cgtggaatgg accttgcgca aaattgcatt
    11761 gcgggatacc accgtcgtcc ggatgtctgc tcgagaggat tattaaagcg agaggttatg
    11821 gaagagaagc gcgatcgcat gatcagagat tttgatattc gggtcgccga tcccaatggc
    11881 ctcatcgatc aattatccgg aggcaatgct cagaaagtca ttgtcgccag agaattcgaa
    11941 agtaagcctc agattttgat tgcctgccaa ccgacacgtg gcgtggatgt cggttcgatt
    12001 gaatttattc atactcaatt gttgaaattt cgggatgccg gaaacgcagt cctgttgatt
    12061 tcgagcgaac tttcggagat tatgagtctt tccgatcggg ttctcgtgat gtataaaggg
    12121 gcaattatcg gtgagatcga tcctcagcat accacaccgg aggaagtcgg gttgttgatg
    12181 gcgggaattg agatcaaacc agtcagtgcg gaacagaaag tgaaacaaga ttaatggaaa
    12241 caatgaatcg gaaacaaaaa tgggcttctt ttggtagatc gttgctcagc tcactggtgc
    12301 cggtcattgc cgcatttata atcggaggga ttattatctc cctgctcggt gaaaatcctt
    12361 ttacaactta ttggatttta atttccaaat cgctgtttac cgtcaagggc tttgcttata
    12421 cactgcataa agccgcccct ttgattttga cgggtctggc aatcgcggta accttcaagg
    12481 cgaatatctt taatatgggc gtggaaggac aattgctgat cggcggtttt tttgccggta
    12541 ttgccggcgc ttctttgacc ggcacatctc ccgttgtcac aaaggttatc tgcatggcgg
    12601 tcggaatggg ctgcggtgtc ctttttgcgt tgattccggc ggttttaaaa gcatatttca
    12661 gagtcaatga aatggttgtg acattaatgc tgaactatgc catgataaaa attctcgaat
    12721 ttcttgcgac aggtgttttc cgagataacg ggtccggata tgtgacaacg ccaacaatcc
    12781 aggccggggc tatgttccgg cgcctcggcg gctcgaagtt gacgccattt ttctttattt
    12841 cgttgattgt ttttttaatc atgtattttg tgatgaatcg ttcggtactc gggtatgaaa
    12901 tcagagctat cggattgaac cctgtgtttg cggaagcggc aagcatgaaa gtccgcaata
    12961 agattataga gattatggtt atcagcggcg cattatccgg attggcaggc gcaggccaaa
    13021 tgatgagtga tcagttcaaa tatacactga ccttttccgg cacgcccggc ctcggttggg
    13081 atggtatgtt ggtttctttg ctgggcaaac atgatcccgt cggaatattg attgccgcga
    13141 ttttttacag tgcgctgaag accggtagcg acaaaataaa cttctatacc aatgttccaa
    13201 aagaaattat agcggtcatt caaagtctga ttatcctttt tttggctgtt caattctttg
    13261 atgaaaagac gaatttattt gacagtctga agaaaaaaat taccggaaga gatgaaacga
    13321 ctgtgaagga ggaactgtaa tggatctcct gcaaagtatt ctttatgaca tggtctatca
    13381 cagtacgccg atcattcttt gcgtaatcgg cggtgtattc gcctataaag cgaatgtact
    13441 caacattgcg ttggaaggta tgatgctctg cggcgctttt atagtgatgt tggttgattt
    13501 tctgaccgga aatctggcac ttgccgttgg cgcttcgatt ttatcggtat tgttactggg
    13561 attgattttt tccttcttcg gaattacctg caaaggaaat gtgattgtgg tcggtttagg
    13621 tattaatatg ttggtatcgg cgatagccgg ctttgtatta aaggtgatga attcttcgaa
    13681 tattctgatt aattcgctga aagtaagtga ctttaaaatc aatatcccgg tcatcgagaa
    13741 tatcccgatt ctcggcagcc ttctgagcgg tcatccgctg attacttacc tcagctttct
    13801 cggcattttt ttgatgtggg ttctgatgta taagacgaaa ttcggcattt atgtccgtgt
    13861 ggtcggtgaa aatgaagatg cggctcgcag cctggggttg aaagtatcct tctatcgcta
    13921 tatggctatc ctgatcggtg ctttttgttg cgctcttgcc ggcattaacc tttctttgga
    13981 aaggttgggt ttatttacga atgatatgac tgccggacgc ggttttgtag cgatcgcggc
    14041 aatttattgc gggcatggcg atcctctcgt ttcggctgtc tatgccatta ttttcggctt
    14101 ggcacgttcg ctctctatca atctgagcat ttttgccggt ccggctgccg gcttatttga
    14161 tgtcattccc tatgttctaa tgaccattgt tttgacggtc gtgtcgattt atcgttataa
    14221 gaatattaag ataagaggct ttcggatcaa tgaataatac cgcactgata attgtagata
    14281 tggtcaaaga ttttaccgac cccggcgggt tggtttatta cccgcagaac cgggaaatcc
    14341 ttccgaaaat tgtcgccgtt cttgaggaga gtcggaattt cggaaaattg gtcatatttt
    14401 tgcaacattg ctatcgaaaa gggaaaccgg ataaaaatct gcgaacgatg cgccccaatt
    14461 gtatcgaagg gacggacggc gatcagattg atccgatgct tcgggtggat ccgatcaagg
    14521 attatgttat tcgcaaacga cgttacagcg gttttttcgg aactgatctg gatcttgttt
    14581 tacgtgaaaa tgaaataaaa aatacggtga tcgtcggaac caaaacaaac tgctgtattc
    14641 gagcaacggt tacggatgca tacaatcttg attacaacgc cattgtgatt cgtgattgcg
    14701 tggcaaccga ttcagaggtt gttaaccaag tgcatctctc tgacatcgaa aaatatttgg
    14761 gccgggtcat tacggcggat gaatatatcg ccgcattgaa agcgggtgaa ttatgagcga
    14821 cgatcctttt gacctgctgg tgagcggtta tgtaagtatg gatcgcatca tcaaaatcaa
    14881 ttccccagca cggattggtt atacgtcgct gatcacaaac cgggataatg ccaaaatcaa
    14941 ttatggcggg tgtccgatca atatttgtgc cgcgctgaat aaactgggga aacgggcggt
    15001 gccggtgata cgcgtcggtg atgattatga agaaatcggc tttcgggatt ttctgataca
    15061 atccggaatt cctttggatg gggtcgagtt ggttcccaac gaaaaaacct cctgctgtta
    15121 tttggtggaa aatccgcagg gagaacatat caccttgttt tataccggcg cgatggatgc
    15181 gaaatacagt cgaccggttc cggatgagct tatcaaacga acaaaaatgg cattgattac
    15241 ggtcggagcc aaacccgata acgaatattt ccttgaaaaa gttcaaaaat attcggtccc
    15301 tttggtcttc agtatgaagt cggatccgga tgcctttccg aaagaattgt taagtgagat
    15361 tctgaaagaa agtctgatta tttttatgaa tgaatcggaa cgaaaaagca tcgaagcgat
    15421 gttcggtttc ggctcgatta ccgatttgtt ctcttttgga aaagcgcaaa tcttggtgac
    15481 aacatgcgga gcgtcgggaa gccgatatta tctgccggat aacggtaata tccgttcgga
    15541 gatgattccg gcttgtcgac ccgatcaggt ggcagatacg accggatccg gggatgctta
    15601 tttggccggt tttatgttgg gctatctgga tcgacgaccg atatcagact gttgccggta
    15661 cggaagtgtc ttggcatctt ttattatcga gaaggaaggg tgctgtacca atattccgac
    15721 gatgcgacag tttatggacc gctataatcg tttcatccgt gaacaggagg cgcagtaata
    15781 atgaaaacac tttatatgaa cgtagactct gccgacattg cgaagaatgt tcttttctcg
    15841 ggtgatccat ggcgagtcga aacggttgcc ggatatttga ccgatcccaa gagagttgca
    15901 ttcaagcgcg aattcaacac ttataccggc acatataagg gaatgccggt aacggtaact
    15961 tcgacaggca tcggtgcacc ttccgctgcc atcgcaatgg aagagatgta tgaagccggt
    16021 atggaagtgg cagtgcgaat ggggacggtt atgtctttac gggaaggaac gctcggcaag
    16081 ttcattatcc cttatgcatc catgcgaatg gaaaaaacca gcgctactta tgtgccggaa
    16141 ggatacccgg cggtagcgga tttggacttg gttcaatgta tgaatcagac ggttaaacga
    16201 atggggggcg aatatattaa cggaatcggt tgcagcatgg acggctttta ttcgatgatg
    16261 catgactccc gtctttcaaa ggaaatgaat gttgacatgt ccgtcatatt cgagaaactt
    16321 aaaaaaatcc gaatttgcgg cgtggatatg gagtcttcct gcattttaac cctcggacaa
    16381 ctgatgggca taaaagcctg tgttctgacc atagtcactg ttttggagaa tctgaaagat
    16441 gcattggaag gagaagaacg aatcgctgca gaagaccttt tatgtcgcgt ggcgttggaa
    16501 ggaatttata actatcgtta ccacgtctga tttagcggtt acgaagaaga ggaaaatatg
    16561 acagtttggg atgtatttag acagaaaagg ccgtttatta tcggcatggt tcattgtcag
    16621 ccgctcttgg ggacgcccgg ttttgatgga agctcacaga atgtgcttta tcgggcgatt
    16681 caggatgccg taacccttga aaaagcgggt gtggacggca ttatggttga aaatatgtgc
    16741 gatgatccgt tgggaacatt gctgactttg gaagaaacgg tcggtttgac tgccgtcgca
    16801 acgatggtca aaaacgtggt gggggatata cctgtcggga tcgacgcggc tttctgtgat
    16861 tgtaaggcgt ctttagcaat tgccaaaacg ctcggctgtc aatttgtgcg aattccggtt
    16921 tttgtggata cggtactata ttccggagga ataatctatc cgtgtgcaca tgattgtatg
    16981 tattatcgaa aacaaatcgg tgcggaagac gtgatgattc ttgcggatgt ccaggtaaaa
    17041 catactcata tgctgatgaa ggatatttct attgaacaat ccgcaaaaga tgccgtagcc
    17101 tgtggggcgg atggcttaat agttaccggt accgctacag gcggagaaac accgcttgag
    17161 atgatccgcc gcgtcaagaa agtcgtcgat attccggttc tggcaggaag cggcgtgaac
    17221 agccgaaata tcaaagaaca gatgagtgtt gcggatgggg cgattgtcgg aaccgctctt
    17281 aaagaagacg gaatattgaa aaacccgatt tcattcgaat tgacatcgga attaatgaaa
    17341 agtttacgaa aataaaggga aatagagatc atgatgcgac caatgaaatt agcgggcagc
    17401 gaattaatgt tcggaagagg ttgccttgag tatatccaca agataccgga aaagcgggtt
    17461 tttattgtaa taggcgggtc cagtatggag aaaaacggca ttcttgcaaa agtggaaaac
    17521 ctttttcatg ataacggagc cgagaccgcc gtttttcgag gagtggaacc cgatccgtct
    17581 tttaaaacag ttcttaaagg tgcggcggaa atgctgaaat ttgagccgga tttgattgtc
    17641 ggcttgggag gcggatccgt gatggatgct ggaaaagcga tgtgggtttg ttacgaacat
    17701 cctgaaattc aaacgttgga tcagctgttg acggccaatc cttttccgaa gttgcgtcag
    17761 aaagcacgtt atttgtgtat tccgacaaca gccggcactg caagcgaagt ttctcgttcc
    17821 gtcgtgataa ccgatgacga aaaagggctg aaacacgggc tcggaaatat ggaaatgatg
    17881 ccggatatcg caatcgatga tccggaagtg accatctttc tgccacctca cattacggct
    17941 gaaacgggaa tggacgcttt gactcatgcg ctggaagcac ttgtctccaa tcgggcaaat
    18001 tatctgagtg atattctggc tgttccggcg gctttggata tcattcgaac gcttccgatc
    18061 gcatacgcga acggaaagga tatggatgcg cgtgaaatga tgctgaatgc ctcaatgaca
    18121 gccggtttgg ctttcaccaa tgtgtctttg ggaattgttc actcattggc tcaaacaatc
    18181 gggggctatt ttcatatttc tcacgggctt gccgatgcga tcatcctccc ctatatcatt
    18241 cgctataacg ctgccgtccc ttatgcggat aagaaatatc atgaccttgc cgcgaaattc
    18301 gggaaaacgg atctttccga aattgttgac gatttgaacc gccggctgca tattccgtct
    18361 acgctgcggg aacccttgca agaagaggcg cgctttgaat ctctgattcc ggaaatggct
    18421 gtcactgcga aaaatgacgg atgcacaaag accaatccga tcatacccga cgtaaatgaa
    18481 tttgccgaat tgttgaaacg ctgctattac ggtgaagaga aataaatcat gaaattaatt
    18541 tgctatctat ccaacgggta tccctccatt gaagagagca tcaaaatggc tgagatttat
    18601 acccatgccg gcgcggatat gattgaaatt gattttccga gccgtaatcc ctatttggaa
    18661 agtgaattta ttgccgatcg aatggccgaa gcgctcaaag cctgcgacga ttatgatgct
    18721 tacatggacg gtatgaagcg ggtaaagcgg ttgctcccgt cgacacaatt tattttaatg
    18781 gtttatgaaa ataccgtcga ggaaatcggt cccgataaat tcatcgattt ctgtcaatcc
    18841 aaccattacg aggatctcat tttggtgggt ctgaaagatg aaagtattaa gaatctctgt
    18901 atccgaaacg gattaaaagt cagttgttat gttcagcgca gaatggaccg aatggaaatc
    18961 gaatatgcca aacaatccaa cggttttgta tatttgcagg caaaagctga tccggatcag
    19021 gttaatccgc tgtacccgac tttaaaagat tgcatcgatc atttacgttg cattggtatc
    19081 gatcgtccga tttattgcgg tgtcggaatt catacaccgg aagatgccga ggcggcaaaa
    19141 gaagccggcg ctgacggtgt ctttgtcggc agcacaattc tgaaacttca taatgatatt
    19201 ccggctttga taaaggaaat caatgaattt aaaaaaaatt gctgatcctt caatttttat
    19261 ttatttttat catttcattt gaagaaggca tttttttgtc gggtcaaaaa ggtcgcttcg
    19321 gcggcctttt tgattgattc cgtcctttgt tgtcggtact ttaatcgagg aatcttaaga
    19381 aatcggtccg atcaatgcgc gaagtttcaa ccggttgttt tatcgctgga aagcaacgca
    19441 actttcattc tattatccct taacgcttcc ggccgttaat cccgtcacaa aatatttttg
    19501 gaacaggata aagaccaaga ttaccgggat caaacttaat gcggacatgg caaatacgta
    19561 tccccattgc gtaaattgat gctggccgaa gaagccggcg atcgcaatcg gtaaagtccg
    19621 aatggcgtta tcgttgatca ctgtatttgc ccagggatat tcttcccaag cggtcaggaa
    19681 attaaaaatg gaaacagacg cgatagccgg tgtcgaaagc gggatggcga ttctgaaaaa
    19741 gacggtaaaa atatccgctc cgtcgatagt cgcagcttcg atgatttcgt cgggaatgtt
    19801 ttccatgaac cctttgatca taaaagtgga aaaagggatg acccatgccg tgtaaaaagg
    19861 aattaatccc caaagtttat tgacgacctt cagttttacc gcaagttcga actgggggac
    19921 aattaatgtg gttccgggaa taatcattgt gacgataata aaaccgaaca aaactttacg
    19981 acccggaaat tgaaatcgag cgatcacgaa agccaaaccg gaagaaatga tggcggcaag
    20041 cgcgacggtc gataccgaca cgatgacact gttgaaaaaa ttgatataga acttattctg
    20101 gctgatgata tattgataat tttcaacagt tacatcctgt aacttgggga aaaatcgggg
    20161 cggatattca taaagtgcac cgttgggacg cagagaggtg ctgatcatat aaatcatcgg
    20221 taagcaagaa agaatcactc cgattgtcaa taaggcatac cgaaaaaaga tgacgctctt
    20281 tttttctttc atccgatttc ctccaattcc ctgtcgcgat tgcgcatagc tttgaattga
    20341 aaaattgcca taattccgag tgtaaaggcc ataatgaacg atacggctgc tgaatatccg
    20401 aatttaccgg tgctgaaagc tttgtaatac atccatgtca aaatggtatc cgtttgatga
    20461 gccggattcc cgccggttat cattttgatg gaagtaaata cattgaaacc gccgattgta
    20521 agcatgaccc aaacaaaaag gatcgttcct ttaattccgg gcagggtgat tttaaaaaat
    20581 ttttgaatcc cgttgcaacc atcaattgct gccgcttcat ataaatcaat cggaacctgt
    20641 tgtaaagcgg ccagaaaaat taccatgttc cacccgattc ctttccatat tccgagaatc
    20701 atcgcaaccg ttaatccgga ccaacgggaa tcaagccaac ggatattaga agcggtgaca
    20761 tgcaaaacct gagaaagaaa ataatttaag agtccttccg tattaaatac ataacgaaaa
    20821 atcagggagg cgataaccca agaggtgata accggtaaat aattcaaaac ccggaaggtt
    20881 acctgaaact ttttaatgcc gtttaccaat atcgccacga agagccccag tatcatttgt
    20941 aaaggtacgg tcaccagtgt atagacgatt gtatttgtga aagatgtcca gaaaaattta
    21001 tcggccaaaa cttcacgata attctgcagg ccgataaatt cttgttgccg catggaagtg
    21061 aaatcccatt tactgaagct catcagaaaa agcttgataa tcggataaac ggtaaaaaaa
    21121 caaaaaaccg ccaaaccggg taacagcagc aggaaaattt ggattttatt tttgtttgat
    21181 tgggttttca ttttttccgc aactccgatt cgacgccgcc ttgagtgaac gtgtcagaat
    21241 cgatcagctc tgacacgttc actcgtttga tttgagttaa aaactttttt attgggtcag
    21301 taattgatcc caaacaccgg ccagatcatc caacgcttgc tgcgttgttt tttcgccatt
    21361 aataattgcc gttaccgcaa catttagttc attatccatt tcggaccaag ctgccaccgt
    21421 cggtctggct tttgcggtct tgatcgcttc caaaaaagga gcaaacgcgg catttttgac
    21481 ggttgcatct tccaatgctg actgattgac cggaatctgg ccgcattttg ccatttcgat
    21541 ttgggcaaat tcaccggtca tgaattgcat gaatttccat gcggcttctt ttttggctgt
    21601 gttgaacatg gcaatatcct caccgcctaa gacagagacc gatccgcctt cccccgccgg
    21661 catcggagcg gtaccgtatg ccaagtccgg ataagcgccc tgaagctcgg cgattttcca
    21721 aggtccttcc agcatcatgg catatcgatt ggtgccgaat ccgtcggtca tcggaatatc
    21781 tccgctgttg aatccggtca aagctccttc tttgtataaa gaaacgaagg tatcaatcgc
    21841 ttttaccgtc gccggactgt tgatataacc ggttgcgatg gtttggtcct catttgtcag
    21901 cgaacctccg aacgaccaaa taaacgggca aaggttccaa ccgtaaagcc cgggttcatt
    21961 ccatccccag acctgctgcc cgtccgcatt ggtgcctgag agcgctttga ttgcggatat
    22021 taattcatcc atagtggccg gtacggctac tccggcatct ttcagcattt tttcgttata
    22081 gaagaggatt ttggtattgg tattcaatcc caagccgtaa taatgatcct gtatttttgc
    22141 cgtgctcatg gcgctttcaa gcagttttcc ggaaacaccg gtgaaatcag acatttcctg
    22201 atccagagcg accaatacgc ccatcttttg gaattcgggc agccaggcga tatcgcagcg
    22261 cgcgacatcc ggaaggcttc cggtaccagc actgatcagg attttattgt gcagttcagc
    22321 ccactcgtgt gaaaccgcat tgaccttgat tccgggattt tcactttcaa acttcggaat
    22381 caaaacattg accaatgttt cgttctcggc ggactgtgcg ctataatggt gccagaaatc
    22441 aatcgtgatt tcttcttggt tttctggcga cattgcaccg gctgcgatag ctgacaggct
    22501 taataagaca actgcgagca tgatcgccag aattttcttt ttctcgttca tatcatttct
    22561 cctttttata tatttgtacg gaataggcat caatcggaat cgatagctgt ccctgatatt
    22621 ccgttatttc ctgattgtaa aaagtgattc cgtcttggag cggttcgggc cggcggatca
    22681 tcgaagaaaa taattcccga tattcagcat cccgatccgg aaccggaagg ataatttccg
    22741 aacaattctc actgttattc ataagaacaa acagtgcttc gtccgatgtt tgccgtataa
    22801 atccataaat atttttttcg tcatcacaaa agtttgagcg gaaggttcct ttgcgtagag
    22861 cggggtgagc tttccgtagt tcgatgaaac aacgatacca ttggaacaaa tctctatcct
    22921 gagattcctg atcccaaatc atacccgccc ggcaccccgg atcattttcg cccgacattc
    22981 cgatttcatc cccatagtaa acggcgggtg aaccgcagaa tgtcatttgg aaagctgcgg
    23041 cgagtttcaa tttgttttta ttttctccgg cttcggaaag aaagcgggcg gtatcatggc
    23101 tgccgatcag gttgtacatt accgaattgg tgaggtcgtt gtatttcgac agcagttgat
    23161 tgatccgccg gtcaaaatca gtcgcacgga tcttcccctt ggcgatccaa tccaaaacgg
    23221 catttcgaaa aaggtaattc atggcgctgt ccatttgatc accgttaccg accaaccgaa
    23281 atgcatctcc ccaggtttca ccgagtaata gaatatcagg atatttttct tttaacacca
    23341 ttcggatagt ctgaagggtg ctcatttcca tttcatccgc tacatccaat ctccaaccgt
    23401 cgattcccgt ttgttcgatc caataaatca tcaccgaaag aataaaattt cgcacatccg
    23461 gatgagcggt ccgcaacttg ggcatatacg gataatcacc gacgcattca tagtttaatg
    23521 ggttggaagt caaaggaaaa tcggagggat aaaaccattc cttgtaggat gaattttttc
    23581 cccgatccag cagatcggag aattgcggga agcttctgcc cacatgatta aacaccccgt
    23641 ccaatattat ttttattccg ttcgaatggg ctaatttgac caaatcttgc aggtcggtta
    23701 acgttccgaa agccggatcg acggtgaaat aatccgttgt cgcatattta tgattgaatt
    23761 cggctttgaa aatcggtgtc agatagatac attccgctcc caactctttc aggtaatcga
    23821 atttttcgat aattccacgc aaatccccgc cgaaataatt atcctgctca ggaaaactgt
    23881 tccaatgaac caaacgggag gattcggggg cgttctgact gcgataaaat cgatcgggaa
    23941 atatttggta atagatgatg ccttttgccc attcggggac agagaacacg tccgcggtat
    24001 tcacatacaa aaattcgaaa aaaccatccg caggcttttc gtcactcaat ccgttttcgg
    24061 ataaaaagcg gatatccccc gcaaaatcga tcaactcgaa atagtatttt atgtaatgca
    24121 ccggttccgg gaaagagatg acggtccgga aatcgtcatg gacggcgtcg cgataagcaa
    24181 ctttcatgtc actgaagcgg acggaagcgg cgtctgtttg atttcgcttc caaaaaatga
    24241 cccggcattc tcgaatgtca cttctggcgg ctgtcaggcg aataaaaaga tcttgcctgg
    24301 aaacgggaag aataaattct cgggttgccc gataaaaaat agccgcttga ttcataattt
    24361 cctgtccgtc gattcgcgtt gaaatagttt ggttccgacc aatatatgct gaatatttgt
    24421 ttcgttttcg attgttttta ataatacaaa agccgcatgt tcgcccaaaa gataggtgtc
    24481 aacgtcaatg gaagtcagag atggttcggt atattgtgct aaaggataat tatcaaaagt
    24541 gacaatcccg atttcctgcg gaatggcccg ccccaatgat ttaactgctt tcaaaattcc
    24601 gaaagcgatc gtattgtcac tgcacaaaaa cgcatcgggc gggtccgttt tttgtaaaaa
    24661 agctgcggcg atccgatagg ctgcttcctg agttccgtcc gattcgatga taaagtcttg
    24721 tcgaggttca atcccctgct tttccaaagc atcacgaaag ccggctattc tgtttttggt
    24781 aaaaatctgc tgttggtttt ctccgacaaa aacgatggac cggtatcctt tttccaacaa
    24841 atgattaaca gcggattcgc tgccttgcgc attattaata tcgacccaat taatctcgtt
    24901 ccgcataaaa ggcggttctc cgagaaccac aaaagggaat ttttgattta agagtttttt
    24961 cagtaatgat atggtgatca gagaaggtgg caaaataatt ccgtcggctt ttttttcgta
    25021 gatcagttgt tcgatcggtg tcatcccttt ggattggttg tgcgtgatga tcaggttata
    25081 tccgttggtc tgggcaatct gttcgattcc gaaaacgctg tgagtgaaat attggttcga
    25141 gaaagatttt ttatcctcgg cgttaataac caacgcgagc gctctggaac tcccattagc
    25201 cagattcctc gcaaggctgt tggggtgaaa atccagttct tccatcaccg caaaaatctt
    25261 attacgcgtc tcatttgaaa ttacggactt tccattaata actcgagaaa cggtggaagg
    25321 ggaaacgccg gcctttttgg ctatatcgcg aatagtaaca gtcattgatc ctcaatgcaa
    25381 acggttgtat gattattttg cagaatttac tcataaagat ttgttgcttt tctaaaatct
    25441 ttaataaata atgcaaccgg ttgcattaaa aataatagac aatcacctgc catttgtcaa
    25501 tagtgagaaa tgtcatattc cgaacagatc ggaaatcatg ttaggcagtg aaataaacca
    25561 aaccgccgtt cattgaggtt gtatggtacc aaagagataa atcaaaggtt attttctttc
    25621 cttcatcgga agcggtagcc aggatggcat cctccgtaat atccgtaccg gtgacggtta
    25681 tccaatgcca attttgcaga tgcgtctcgt ttccgcggga aagaaccaaa aatcccagag
    25741 gactgtctga agataaccct ttgcttacaa aatcagcaag cccctgcagc tcattccgcc
    25801 ggccgttgtt cttttcgcct acctcaaaaa catgggggga taatgtgatc cctttttcat
    25861 tggcgaactg aattacgccg tcaacatatt tactgatttt atccacaccc atcatgccgg
    25921 gcttaataaa gcgactcatt tcttgcatct gacggataaa actatggcgg gtcagatcat
    25981 ccgcaatata aaggctgcga agttcggggc gggtaaatgc ataataagcc atcaagagag
    26041 atgcggaggt tggtccgcat cctgattttc gtaaccaacg atcgggatac caaacctgat
    26101 ccggaccgaa agttgtttcg gcgtaggttc cgtttcttat ttcaagaagt tggggatggc
    26161 tgatctcgta gtgcatattg actaaaactt tacctttttc ccgaaaactc aggcgctttc
    26221 tgtttcattt tcttagtaag caacccgata ttatacaaca gatcacgctc gatttcgaaa
    26281 atattcgaaa tattaatgta tggaaataag aagaagaatt actaaagaaa taggattaac
    26341 ttaaaatcgg acacaaaaag gctgacagat attttcaacc tttctgtatc cgtttcatga
    26401 tcaatttact cgaggggaaa atccgcagat aaagaatatc gatttctccc cgatgttaat
    26461 gtccccttaa tggaatatag gagtaaaaat cttcagctat ggataacatt tcttgataaa
    26521 gatcatataa tttcggatca ttatcattac tgatacactg aatataaaat tcaaccgcgg
    26581 caatttcatg ataagccccc ataaaaatat ggtaggcata atcgcttaat tcttcgggct
    26641 cctgtgccgc catgatatat ccgcaggtca caccgacatc ccagcgcaga ttgtcccgga
    26701 tgctatgcca gttttcagaa atggtctgaa tttctcctga ataaaccgag tccaaatcct
    26761 ctttataatt atcaagaata gaaaagcatt gctcaagctt cgcgtaataa tcatcatcgg
    26821 ataattccgt ttcattgtcg atctgccggg aacccgaaaa agcacttacg ggtgtaaaac
    26881 acaggatcat caaaacggaa acaaaactaa aaataatcat ttttttcata atttattcct
    26941 tctcttcctc aatatagaaa cggtgagcca tgattttcct tgtattcttt gatgatttcc
    27001 acaggattca atgatgactc accgttttga aaaaagattt aataattaaa agtgatttga
    27061 cgtttatacg gctattttgg aatgatcgag taattatttt gagctgcttc ggccatctgt
    27121 tggtacaaac tatacagcgt tgggtcatta ttgttattaa tgctcaaaat atacaattca
    27181 atcgcaacca actggtaata agctccgtag acgagttcgc ttttgtagtc tttcaattcc
    27241 tccggttcca ttattgacat aatataccca caggaaaccg ctgaattcca gcggagatta
    27301 tttcgggtat ctttccattt ttgagtgagg gtacttattt ttccggatga aaaggaatct
    27361 aaatcatctt tgtacaaatc caacaaggca aagcattgat tcagactatc ataatataac
    27421 tggttgtcat cggactgggt ttctacatca gtttcctccg tttcggtttg agctgaggca
    27481 ggatagaaag ccgtaaaggc aagtaccaag gcaaaaaaga aaatgaaaat ctttttcata
    27541 tcttttcctt tctaattgtt acctataaaa ataacaaaac cgctcttttg atgagtacaa
    27601 aaacggattt ttgaatgtta atccgaaatc tgtcagtcga tcaaaggaac ttttaaactt
    27661 tgaaaggttt ccatatggaa ggaaaactcc ttccgaaatg atatgattgg tatttaactt
    27721 tttctgattc gataaccctc ctttttttat tttattagca gcggaatatt catcatttgt
    27781 gaagttggtt attttttatc tttgttcccg aattaatgat taatccgatt aacgacaaag
    27841 agtaaatttt tttatcaatt ccgatttttg agtcgattca ctcaataatg cggatttttc
    27901 gatagcatga aataattcag tattgatatt tatctaattg tacaagatta ttgattgact
    27961 gtatataggc aatatgggat gtaatagccg ttgctgcatt ttaaaagtgc acattttccg
    28021 ctttccgtac aatgattcag aacatcgtaa gactcccagg aaagattttc gacaaattga
    28081 ggaatgagca aataacggat atgactttgt gagagctttt gttcgaagat gacggaatct
    28141 ttaaaatcga ctttttcgca gcaatttccg tatccgggga gaaaataggc cggataattg
    28201 gtcagaatat cggcggtttc atgcgtttca cccagcattc catataataa ttcctgattt
    28261 aatccggctc cgaacaaatt ctttttttga ttatcctggt cttgacgtaa tttttcatcg
    28321 gggaagaggt tgatgatggc gatgattccg attacggtga tgagtaggaa aggaatcccc
    28381 accgcaatct ttccggcgcc ccatctctcc gcaaggatca gcattttttg aatgaaaccg
    28441 tcaactgcaa gaccgcaaag aatcgccaaa ggcggtaagg ctaccgctag atttcgcgta
    28501 tcataagtaa aaaacatagc ccaaaggatg acgatcggcc aggataccaa gaaaaacgga
    28561 agccgatatt ttttcggaac aaatggaagc ccgatcaaac tgatcccaaa aacatagaag
    28621 tacttgccaa gcgattgtcg gattaaataa aatttatgcg accattcata ccgttgatta
    28681 aattgaacaa tcccttgatc aacaatccgg gtatcggtat caggattcat catccattga
    28741 atgagtccgc cgaaatacca agggagaaca attccggcac aaacaactat ccatccgatc
    28801 ttctgttttt gcggaatttg ctgttcggca gctttcagcc atcgatgaat gaaaatgggt
    28861 actacaatca acgcaatgag tccggattgt ttggttacgg ctgcttcgga agcaaagact
    28921 aatccgagaa cgaggctttg ttgctgatct tttacggatc gatcgctgcc ttttaagatt
    28981 gcataaaggg acagaagaac catgctggct gcgggtacat ccatatagcc attaaatatc
    29041 tgatccccca tcaatttttt catcatatat cgtgcgataa tcgcagcgaa aaaaaagccg
    29101 gtttctttct tttggaaacc cagatcaaaa agcatcagga aaatccacag aaagaataaa
    29161 ggcggaatga gtgtgttaaa gaattgaacc gggttgtcac cctgcagcac ataacttatt
    29221 gaccaattgg cagcgacaag ttgcggatag gatccgcgaa aagccggaat ttggttggtt
    29281 gcccatgttt ctgcaaatgc attccacgag aaaagagtat cccatccgct gaaaatcgtt
    29341 ccgaaattca agcgccagac atggatcccc cataaaacgg cggagacgga accgagaccg
    29401 cttaataccc acagtgcatt ttgtataatt tcttcgctgt tatggaaagg ttgggaaaaa
    29461 gctgttgtta aaggctgcag ctcctgtttt atggtttttt cagccttcaa aagaatctct
    29521 ttcagcgggg ttcggaatga tcgccgaaaa agaaacaaca aggcaaagat ttcaaaagcg
    29581 atcaagagcg atagaaccgg tcgggtataa attcttaaag cagcaagcag aaaaatgacc
    29641 aaatagttgg taaccagact taaagggaac aaatagagaa aacgttcaat taatcctccg
    29701 cgaattttaa aaaccttcga gagaattaat cccggtaaca aaacgcattg aagcagtccg
    29761 atgactccgg aaaaaattaa catgaactat tccctgttta aggtataaat aacgccgtca
    29821 tccgtatccg caaccacttt caaattttga aacaaggcat cgatcctttt ttgagtatcc
    29881 tgaccgtaaa tatctctcat ctgatcttca aaagtatcga atacgaccag gaatgcttcg
    29941 ccccgttgaa aagcggatct gcctgcgtcg atgtcttgag gattcgggct ttcatatgca
    30001 taaaaattct catccggtcg attgacataa acttcatgca tcggccaggt tattcggttt
    30061 aacaaaaaca gtagcgctgt ttcttcattg gtgacaatcg gttgttcggg cggaatgtgg
    30121 tcacgaatat aagccactgt ttcggattct ctccacttga ccgaattgta acctaatccg
    30181 tcgatcgcat tctggcgggc gatacgaatc gatcgcaatc cataagtcgc agccaaaccg
    30241 atcagacaga aagtgataat ggttttcaat ccggaagagt tttgccggtg ccgcaataat
    30301 tttgcataaa tcgaaccgat caagcagaga aagctgaaat gtgccggtga aagcattcgc
    30361 gatccgatcg taatcggcgg ataagtggtg acggaggtca gcagaataac aaaaagataa
    30421 atataaaaaa atgaacaaaa tatcatcgac aagttattga gaattcggtc gttttgatca
    30481 ctggtaaccc gatgcggaat gccggtgcag atgaagaacg gaagaacaag cagcagataa
    30541 tagactatag agttacccca agcggggatg gatggtgaat ggatgattga atccggaaaa
    30601 agccattgta aaaagaccgg ctgcagctga ctattaaaac gcctgatttc ctcccacaaa
    30661 tttacatttt ccaagatact gcgggatgcc accgtcgaag tcattcggat atcataaatc
    30721 agccaaatca gcatgggaag aaaagcgatc aaaccataaa caacggcttc gctcaaacga
    30781 atgcgaaatt gttttttctg agcaaacaac aaaatcaaac aagcgcagat caaacaagct
    30841 gccatactgt aacgggtcag aaaagaaagg ccgcagctga ctgcgctgat gaacagccgg
    30901 ttacgctgtc ccgttgatag atattgtgcc aacgtaagga atccgattgc catcaatgca
    30961 atgctcaaag gttcggacat agcccaggaa taaaccggta tcaaaatcgg tgaagctgcc
    31021 aacaaagtgc ccgtcagaag tccggcaatc catttttcgg cggattgtga aactgttttg
    31081 gcaaccacga aaacggtgac ggcaaacaga atcatattga atggtccggc aaagccggcc
    31141 ggatcgaaac caagccaacc gaaaaatgcc aacaaaagcg gatagaaagg aggaaaatat
    31201 ggaagaagcc tgaattctcc cgccggattg atcagcccca atccttttcc ggctaacaga
    31261 tttcgggcgg cagtgatgta aatcgtggca tcgcctccca ctcccggtcc ccggcgcatg
    31321 cttatcgcca gtaaataaca tccgagaacg gcgggcaaaa cagccgatcc ccatatcgct
    31381 ttttgattat ttattttcat catcttcttt aaaaccatac accgacagat tatcaaaggt
    31441 agccaattca accaaaccgt tttcccggca ttgatttttc agttcttcaa gtgtctgcca
    31501 gcgagcggtc ggcttttgcc cgatcagaga atccagcgct tctaaatcga acaggaagaa
    31561 atccactttc aattctcgag ctttttcgag gctttccgtg ctcgggaaag tatccatgat
    31621 ctctttaatg ctccggtatt gcgatggggg aaaggcattg aagaatccgc cgataaacgg
    31681 tttgttatta tataaagtat agtatgtggc agcctggtca tcattcaatt caaagggaat
    31741 gttcatgacc gaaccgcttt cggggcgttc cgataaccag agatcgaccg gacgcggttt
    31801 aatttcggaa aaccggtcaa tcggtctggg ggcgaaatcc aggataatca gcgtgattaa
    31861 tagaaccgag atgaagatgg atcggcttcc ggttctttcg ataagagctt tcgcgcccaa
    31921 acccgctccg caagcaataa gaaccaatgc aaacagtccg aagcgcatta atgcgcgcat
    31981 ttttccgaag aatggaaaat atttatatag aatcaatccc ggcagataaa ttctggcaga
    32041 ttctttttgc agtatatttt gcaaaaatcg gggcagagaa acaatgacag gctgttcatt
    32101 ccaatgtaaa tcggttccca tagctaaaat taccgcgcaa acaatcccga ttgtcagggc
    32161 tgaccaaatc caattttgtt cttttttctt tcgaaccatt ccgtaaaaat aaagaatcaa
    32221 cgaaatggtt cccgcgtaca atgtggcttc aatccataaa tcacggtgga aatggctgcc
    32281 gacccatttt ccgaaaatga aatgggttgt ggcgggcaat aagaaatcgg tcggacttgc
    32341 cgaatacaaa ctggcgctgc cccagtctcg atcaggcatc cctccggagc ttgccaaggt
    32401 caaaaatggc aactcggcaa ggatcaaaag cgacacagcg attaatagga aaagtccaaa
    32461 ggttttccaa aaagccatat cttttaattg agaaattcgg ttcggaagaa tgagcaaaat
    32521 cccttgtatg gcggcgacaa aaatcaacat aaatacatag tattgagagc agaatgcggt
    32581 caatcccaac cccagagctg ccaatattgc ggatttcttg cgaaattccc cggtacccgg
    32641 tcgcagcata tcaaaccagc cccagaagaa aagcgggatc cattggattc cgcagaggtt
    32701 caaatgaccg gccaaaaaat gggaatgcca atagggtaaa caggcaaaga cggtacctcc
    32761 gacccagcct gccgatgcag acccgctcaa atgcctgacc caaccgaaca tcattgttcc
    32821 ggcaaggatg aaactaagca gcatcgccat gttatagccg aaagtttcgc cgccgattaa
    32881 actgaagggt aatgccaaag ccaattgcgc cggagccatc tcggttcgtg ccagattcca
    32941 accctctgga taatttaaga accagacatc caagggattg gcatggatct gaaaaagcgc
    33001 ttttttgacc caaccgatca tccaaataaa ataaatatta tcgccgatgt ccccggctat
    33061 ttcatgggtc aggtgagtta ccagcggcca ggtaaaaatt acagacagga gcacgaacca
    33121 caaaataata aaaaaaagag ttcgttttct gaaaaaacta accaagattt tgttccttct
    33181 taaaatcgat gatccgtttt tcatcccagg aacgcttcac cgcgttgatc agttgttccg
    33241 taattaaccc gtcttgaagc gtacaagagg gttcgacctc tcgttttgta accgcaataa
    33301 aatgtttcat ctcttcgaga aacaattgat tgcgttcaaa cggttcggcg ggagggataa
    33361 taaccgattc cggatagact gcgttttgca acgtcacgca tgcgtcggaa ttgttccaat
    33421 gtaaaacccc ttgcgtaccg gtaatttcca ggtcattgcg cacaaatcgt cgaaagtaat
    33481 ccaaatgaat cgatccgacc aaattatttt ccattttcac gccgacttcg gcgacatcat
    33541 caacggaaag ttccaaatca ctgattttgc cgttatagcc ccagatgaaa tccacttcac
    33601 cgaaaagcca acgaacatag tcgaaaggat ggcagagggt caaagccaca ccaccgccta
    33661 aatcttttcg tgcactgtaa ctttttcgat aatcttccca cggatgccaa tccggcaggt
    33721 attcgctcca acaggcttga aaagaaagcg gtcgcccgat tgcaccggtc tgcatcaatt
    33781 cctttatctt gattaagccg ggatgaaagc gaaattgaaa acccatcaat aatttcccgt
    33841 taccggcttt aagagcggac tccaattcgc ctattttttc gatttgatcg gtaatcggtt
    33901 tttccatcag gatggaacag ccggcttttg ctgcgggaat ggcgacatcc agatgaaaag
    33961 cggtcggatt ggcgataatt acgccgtccg gatgatgagc catcgctttg tttatttctg
    34021 tttcaaccgg aatatccccg atttcatcgg tcggtaaggt gctgtgattt gaccgcagca
    34081 gcaccagatc attttgtccg catgcacgaa ggtttctcaa atgccttcgc ccgatcgaac
    34141 cgaatcccgc aattaaaaac cgcatatttc atcccttctt taccgctgcg ttagatttgt
    34201 tgcatttatt ttagcagaaa ccattcctat tttgatttcg attcgccggt ataccggtac
    34261 gaacgatctc cgttttgtac cagaatcaga tccgcgactg tggtggcacc ttgtgaatgg
    34321 cccaccatcc agatttcgga attatcggga aaataagcgg gaacggtttc ggttcgaagc
    34381 tcgatcgtcc cgaaaccgcc gggagaaagg gtcagaaaaa tgagccggcc ataatcggaa
    34441 actccgtatc cctgttttgc gctttccggt tcaccctctc ccgcggcata atatcgcggg
    34501 taaatcgcaa tggctttcat aacaacagcg tcagcggtat cgtagtcatt cggatcgact
    34561 tcgagttttg aaagcacagc ttcccgggat gccggttcga atctcatcgg aatgattctt
    34621 tcggaaagag gaagcgataa accaatcgct ataaaaatcc cgagatataa accgagcttt
    34681 cgctttggat gaagtttggc gggctgaacg aattgggcag cttcccgttc ggtttcttcg
    34741 ttggatgtct gcggcggagt aaaaccgctc agtcgaaatg atcgatatag ccattcggct
    34801 attccgattc cgccgtataa aaagatgatc caatcaaccg ggatcagata tcttccggca
    34861 gaataacgtc cgacggcggt gctgagatta aataacagga tcaccgccaa cggtaccaat
    34921 ccttcgcgca tatgaccttc catggaagct gagataccga atgcgagcag tgcataggat
    34981 aatgccaaaa agatcaggtt atagatcgat aattgatcat tgtttatttc ttcccaaaaa
    35041 ggtgtcgtcg gcttgattaa ttccatcggg cggcttaatc gatcccgtaa cgggaataat
    35101 cgcaaattgc agatttcgtt attgacaaaa tgcgcggtta tgaaagttgc tatttctttg
    35161 ggatattgtt tgacagacgt cagaattgaa cgggtcagtt tttcggaata ttccccttcg
    35221 ttcatccctt catcgtttgt cagatccgtt cctcctatat tgtagctgat cgccatctcg
    35281 gcggtttggg tagccgggtg atcaaaaaca aatttaccgg ttatttgttt attgcgaatg
    35341 agccatggga ataggcaaca acagagggcc gtggtaaaaa gaatgaattg tgagagacgc
    35401 cggctaaatt gctttcgggt ccggataaac aaaaaggcca gagggacgaa aatcaatgaa
    35461 agactctgag tacgaatcag cgtcatcatt cccaaagctc ctccggttaa taccgcataa
    35521 acatttttac gataattgtc ttcgcgttga gctttcagcc accgtatcag catcaatacg
    35581 aagaacgcag cagccaaggc ggtcggtaaa tcagcaaaaa agtacttcgt agtggagacg
    35641 ttatgcgcaa aaggtgcgct gaggatggag ttggtttccc gtaaaataca cagcaaagct
    35701 gccgccaatc cggctccgat actgtgcagt tctttgccga tcagataaat caaagcgggc
    35761 aatatcccca atacaagggt ctgtaaaaga atcaccgagt catactgatt tccgatcagc
    35821 agatgaaaaa tggctaacaa tacaatatat aaaggccggg gaggaatatc tcggccttta
    35881 aaacccatcc ccgcagccaa actgcgagca taatgtccgt aaaaggagcc gtccgaaaaa
    35941 ggataaactt cgaaattcgg cgctctgccg ggcggagaga aaaaaccgtg ttgatttgga
    36001 attgccagcc aaatcccgac cgcaaccgcc caaacaatga acggcatggc cttgatcaca
    36061 acgggagcgg ctttgcctat ttgtttcatt tcaagaatta tccacagaag acaaagcaag
    36121 aatgccaaaa tcagatgcca ttcaagcaaa gggacggtcg gcttcccaaa aaaagcatta
    36181 tccttgacta agccgatccg ggtaacggcg attccgaccg cagcgacgat gccgattccc
    36241 atcagaatca ggaaggtttt tctttgatga cttttttgtc ggaaaacttc ccgaaaatca
    36301 tttgaaattc gataaagaaa atataaaatc gcttggattg caatgaatcc cagccaacaa
    36361 accggaggga acaagcgcct gtaaaccacg aaccaagaat aatcttcact tgttcggtac
    36421 agcaaccaca gagatcgcag taaagccgtt cccgacagaa gaattccctg gcttaaaagt
    36481 aatgcgaaca aacgaaaagt tttcttggcg cttaaacgaa tccaaaaatt cagcacattt
    36541 tttgaaacca gagggattcc gaaaaaacag gccagcaaca gaaccgccga gagcaaagcg
    36601 agtcgaacgc gactgaacgc gaaaaggctg ccctctccgc ttcctccggg aagaaacaat
    36661 aaatacagga agcacaggat cccttccgcg gaggatagca tgaaaaaatg ttcggttgct
    36721 tttacatcct tgttcattgt tgaaatttat ccgaatgaat gatcaattct cgattttgtt
    36781 atctgaagaa atggatgctt gttttcccga cgcagaaata gcgatcggtt tttccagccc
    36841 ttgatttaaa cttccgcgcc gatatcccgc taagtccaat gtggtatgca aaaatccgat
    36901 ttcgttgaag attctgacaa tctgttcccg atgatccgca atgattttca tatctctttc
    36961 cggtacttca attgcggcag cggaatcgta aagccgtacg cggcattctc gaatcccgag
    37021 agattttaaa taattttcgc cttttccgat ttttatgaga tctttcggat cgattcgtcg
    37081 accgtatgga atacgagttg ccagacaggg agtggaaggt tgatcccatg tgttcagttg
    37141 aaaagttttt gatatctccc gaatctccaa tttagtcatc ccggcttcga caaacggact
    37201 gcgaacgccg cattctttta tcgcttctcg acccggacgg taatcaccca gatcatcgat
    37261 gttctgtcct tcgattacaa atgcaaaatt ccgttccttt gcgatacgga tcatttccgt
    37321 aagaatttgg cgcttgcagt aatagcagcg attcgacgga ttggacgcga tcgattcgac
    37381 tttcaacaga ttctgtttaa cgatttcgtg agggatttga tgttttttcg caaacgcttc
    37441 ggcagtatcg atggcgtccg gcggatccaa ttccgatgag acggtgatcg ccagacttcg
    37501 atctcccaag gtctcatggg cgagataagc caaaaggctg ctgtccacac cccctgaaaa
    37561 agcaacaata accgatccca tttcccgcag gatttcttgc aatttatcca attttgactg
    37621 tatttcagaa gccaattccg atttctcagt catcttgcaa gtccttttga actctgagat
    37681 gattgatttt ggaagccaga caagccgcac cgtatccgtt atcgatattc acaaccccga
    37741 caccgttggc acagctgttt aacatcgaaa gcaaagcagc taagccgtga aaattggaac
    37801 cgtagccgac gctggtagga acggcaatta ccggacattg aaccaacccg ccgacgacgc
    37861 tggcgagcgc tccttccatt ccggcgacaa cgatgatgac ttttgctttt cggagatccg
    37921 gcagacggtt aaagagacga tgaatacctg aaacgccgac atcgtatatg cgctcgactc
    37981 ggtttcccat cagttccgct gtcagagcgg cttcttcggc aaccggaata tcaccggtgc
    38041 cggcggtaac taccgtgatc ccttccaata attcttgctt ctccttcgta tatagaattc
    38101 gagcagtggg atcataggtg gcatatggca ggcgttctcg aatatgttca taaacttccg
    38161 gactgattcg cgaagccaat acatttggat ttttatcatg taatctttca aatatttcaa
    38221 cgatctgttc ttccgttttc cccggtccga agattacctc cggaaatccg gtgcggattt
    38281 cccgctgatg atcgattacg gcaaaattcc ctaccgattc ctgcggaaat tcgcccaacg
    38341 cttttatccc ttccgaaaca gatatcgcac cgcttgcaac ggcttccata atctgtcgaa
    38401 tttgttgatc attcataaaa attctccgag ccgacattta gtttctaccg tttaattcta
    38461 ttcgataatc cgtcaggcgg gatatatggg attcaaaaga tttccgcaaa agtcgattcg
    38521 gatcattatt ttttgatcga aaagagtcgg gatcttttat tttggtaatc tcgcgggtga
    38581 agcggatagt ccatcttatt ttatagttgc gatatactaa aagcgtagat atattttatt
    38641 gaataaggcg cagcgatgat tcggaaaaat tttttttgtt accttctgat ccctcttagt
    38701 gtgacagcct gtgttcagca cctgccacgg gaaattgtta cctctacggt gccaaacgag
    38761 acttccgttg cccaaaccac agcggatacc gcagaaactc cggttgcggc tgaggaatat
    38821 gctcttccgg aaggcactct cgtgtggacg gatgcatcga tgacccgcac gttgccggcg
    38881 ggagcaactt atgccgatcc ttgggattat tgccaggcgg ttggaacgat tgatacaccc
    38941 ggacccgaat acacgggaac gaatcccccg gaagtcataa aaaaagaagt attacgcgcc
    39001 ctggctattt ccgaatcgga tggcacggct cataatgtgg tatggcgttg tgtggatggg
    39061 caagtgtttg gctgcgacag ttcgatgtac tcgacttgcc tgactccgat gaatctaagc
    39121 acgaccccgc cggaagcggc tgtcaacgaa tgtgccaagg ctgaaatgga taatataaca
    39181 ttgccgggtg cggtcgtcgg gaacgacact ccttacgaat ggacctgcca aggcggaaag
    39241 cccgttatta ccggtcaggg ggtgaccgtg gatgaacagg gatataacgc actgatttgg
    39301 tatccggtac cgagacaata aaccttagcg gagttttttt tatgaatgcg gttgacatta
    39361 ttattaaaaa aagagattcc aaagcattaa ccgatgagga gattcgattt tttattgacg
    39421 gttataccga agggaaaatt cccgattatc aagcggcagc ttgggcgatg gcggttttgt
    39481 gccgcgggat gaccgctcgg gagacgacgg ccctaaccag agcgatggcg gaatccggcg
    39541 agatggtatc tctcaagggc attgcggata tcgcggttga taaacattcg accggaggag
    39601 tgggcgataa aacgacgctg atcgttcaac ccattgttac ggcttgcggt ttgccggtgg
    39661 caaagatgtc cgggcgggga ttgggattca gcggaggcac tattgacaaa ttcgagtcta
    39721 ttcccggaat gcgactgaat ttatcgaaag aggaattctt aaaccaactg aaacaatatg
    39781 gtacggttct ttccggacaa tcggcggatc tcgctccggc tgacggaaag ctatatgcgc
    39841 tccgagacgt caccggaacc gttccgtcta tccccttgat tgcttcatcc attatgagca
    39901 agaaaatcgc cgccggaacc ccgcttattt tgttggatgt caaagtcggg aacggcgctt
    39961 ttatggaaac acttgaaaaa gcggagaaac tggcaagatt aatggtgtcc atcgggaaga
    40021 agttcgggag aagcgtgaaa gccgaattga ccgatatgaa ccaaccgttg ggttacgccg
    40081 tcgggaatgc gttggaagta caggaagccg tgcgtatgtt acgcggagaa aaatccgctc
    40141 cggatttata tgagcattgc atcgaatctt ccgcgttgat gattaaaatg ggtaggaaag
    40201 cgaaatctgt tgatgaagcg cgtcatatgg cagaagatgc gatccatacc ggaaaagctt
    40261 tggagagtct aaagctgttg gttcgtctcc agggcgggga tgtttcttat atcgatgatc
    40321 tcgctaaatt cccgaaggcg cccattattc gagatttata tgcccgatcg gatggttggc
    40381 tttcggaaat caatgcccgc atggttggtg aagcatccgt tttgttgggc gctggtcggg
    40441 ttaccaaagc ggatcgaatc gatcatcgag tcgggattat tgtgcaccat aaagtcggcg
    40501 atccgatcaa aaaaggcatg ccgcttttta cggttcatgc caacaatgag gaaaagctga
    40561 atcaagccgc tgaattatta aacaatgcct gtaagtacag tgaaagcgaa gtcgaaaaac
    40621 tgccgcaatt ttacggactg atcgaatagc ggaagcctat ggtttttcgg ttgaaatttt
    40681 ttcctcgggg gtttgcgtaa cggcgggtgt taaagtgatt tcatcacccg gcatacgttg
    40741 ataaggaagg gtcgggcatt cgatcttaag gtatgggttt gcccggatca cttcgattgc
    40801 cggcatattt atatctgaaa gggaaacgaa atccggtgaa tccggcaaac ggactcgaat
    40861 ccatccggtg ttgcgatttt tactcggata aaagattaat gcccagattt cgcctttgtt
    40921 gaaatcgggt tcacaatacc gaatcacatt caccttggtt cccggttcca aatatccgtc
    40981 gctgatcata actgacatgc cggaaggaag ttcataaacc ccgacacggt tattattgat
    41041 caaacctgtt aaccctaccg gtgtaggcgt aaacgtgaaa gtggctgtgg gcgtcatcgt
    41101 cagagtcggt gtcgcggtta atgtaggtga cggtgtaacg gtgtttgtca gagtcaacgt
    41161 cggagtttcg gataaagtcg gcgtcaggga aagagtcggc gaaggtgtca acagtggaat
    41221 caattttggc gaagccatat aaagtgcgcc cagaattaag ataacggaaa tcaatcctcc
    41281 gaccagccga tgttttctac ttgctttttt ctttcgcgtg tctttataac gttgtaagga
    41341 ctcggcttcc cgcggaagaa gatccattcg ttccaattcg cggatggtat ttttaattgt
    41401 ctgtccccgc tcactccgtt tttgtcttga tttcttcggg ctttcattta attgggtcaa
    41461 taattgatga gcttgcgttc gttgctcaag ccatttgttc cgtgcggcaa aacccggctg
    41521 aattccgatc aattccgcgg gaatttgctc gaagaaatct cttgcgtttt ttcgaattgc
    41581 gtaaatggca tccagactga tttccgatcc gcgaatgcca tatccgcgga tcgtgttatc
    41641 ggacgattca acccaattca acaaacctaa caaataatta atttgcttga tataaggatt
    41701 tttttcggga tcgactttga tttctttttc caaaattgtt ttatattgat ttaattcatt
    41761 cgaaagttcg gaaatcaatg cgggatcgga tgttttaggg tccaatatgc gattgacttt
    41821 ttcaatgacc tccccatttc ctgacgaatc tttcgtgaaa cggctttgga tcaggtgata
    41881 tttttcgctg agatcacgga tttcattttc ggcattttct ttttgaatct taagccaatc
    41941 cggccattta tcttgctctc cggtttggaa ggagtttagg attcgttccg cggatcgaaa
    42001 atctccctgt cttacgaagg aatttgcctg ttcgatcatg cctgaaagtg cgcgaaaagc
    42061 gttgttgatc tcatcgagcc tcagtttcaa ttcaaggatt tgctggctgc gattttcgac
    42121 ccaaacccgc gattccattt gtttcaggat gtcggctgcc cggtttaagc cttgcggccc
    42181 taaaatcagg ctgttattga attgaatctg caggtcatta aaatcgacct tctcttgatt
    42241 gattattctt tcgattcggt tttgtgcagc cgcgattttt gcccaagttt ttttatccgc
    42301 gatttgacgt tttcccgatt gattgatttt ttcagaaaga taacgaatct cttccgcttg
    42361 ttcttctgtc aacttgccgg actcaccgat atcgccggaa aggaagtcat ctatttcttc
    42421 caataaagcc cggtcggctt gttggcggct gataatcatc ttctggaatt gtgcggcttc
    42481 caatgtgtag aagaaatcca acgatttggt aatcgcttct tccgcatcct gataatttcc
    42541 gtcggcaaac gcatctttgg cattccgaag ataaaattcg gattgcgaaa tacggtagag
    42601 ttgttcgagc cgatccgccg cttgttgaat ttgcggattt ttacggtctt cggcggttat
    42661 gtttttcgca atataatcga ccccggccgt atacgcttta tcgttttgaa aattgtgcaa
    42721 cgagttcaga atgaaattta cccgttgacc ttcgatatat ttattattga aatcgaccct
    42781 gaattgactg actgcttcaa aaagattggc atcgccggaa tcttccgcga atttttccgc
    42841 ttttgccaga taatggggaa tattggattg attgttgtcg gatttggcac agaaaaacca
    42901 aaattctgcc aaaattttct ttaccggctt taattcgata ctttcactgt tctcgaacga
    42961 ttcagcggtt ttcaggcttt caatggtgat taaaccatgg tccatcgctg ctttcagcga
    43021 ttcaaaatcc gttctttttg actcgatcag ttgaagctgt cggatcccgg attcttttcg
    43081 ctcgattaag ctttgcggtt ctttaaccgc attttgagaa tagacggatt cgatttcccg
    43141 aaagatgtct tcatattccc gaaccgaatc tctgtcgctt gaaataggat taagcgcatt
    43201 ttgtaaaatt tcatccgctt ttttcagcaa attttcgact tggttgtttg catcttccag
    43261 ttgattgata cttgtttggc gattccgtaa ggtcctcttt tcatccgtat ccaaattgag
    43321 ggaattaagc tgatccagtt tctcttttgc cgcggccgga tcgcctttgt tgattgcacg
    43381 atcgatttcc gcgagtcctt tttcaaatac ataactttta acggaatcat atcgtttatg
    43441 taattcatca cggaattgag cgagtccggg agtatgagag tctcgggcaa aataatcgtc
    43501 aatctgatta acccaggaca gtaatttatt caacgtgata ttgtcatccg cgttatattc
    43561 gtcacgaatg attcggtatt tctcggcgat ttcatttcga acggataaag catcggcata
    43621 tcgttcttcc aacgaaagga ttttatccaa cgtctcggtc ggaagactga cttcgccgag
    43681 ataatcgccc tcgtcatatt cggcggccgg ttttccggtt tcctttgcca aacggatatt
    43741 aatttcggcg gagcttaatt ctcccgcatc cagtgccttt tccgccagcc ggcaatagga
    43801 atttgcccgt tttgccactt catcatgaat atcggtttga atcttttgat tcttttcccg
    43861 taattcattg atccgagaat tgatctgaat tgcccgctcg ctgtcgggag gttcctgttg
    43921 cagttcgctt gtaagcagaa tcattaaccg ggcattgttg ttgtatagta cccgcagatt
    43981 cttactttcg gcatcttcaa gggaacggat ggattgctca agacggagcg tttcggccgg
    44041 gattccgtca gggcgcaatc ggtttttcca actttctcct tccgcccgcg taatcatttt
    44101 aagtaaatct tcccgatcgt gatccgcatc gaaaagcgca atcccgtatg actccaaacc
    44161 gagtgaagaa tattcattaa tcagggacat tgccttctga taagaagcga tgcctccttt
    44221 ttctcggagt gcgctgactt cggtttgaag ataaagccca cgctcgagct tttcctgcaa
    44281 ttcggaagtt ttcaatattt tttcacgcag ctcgggaacc cgatcccaat ccggatatat
    44341 ctgtttggcg gtctcaagcg cttcggcggc tttggcgctg ttgaattcga tgacgatggc
    44401 attataagct tcgtcaactc tttcgaatgc ctgcttccgc gcaataacgt cccggacggt
    44461 gttcaatttg gcgagtaatg attcatatcc cggataggta atgcgaatat cttccggaat
    44521 ttgatcgacg gcatattggg cggcgtccgg gtcttcttca acaatggatt cagcttcatg
    44581 gaaattgtcc agatatccct ggtagcgttc attcaatccc cgcaattcga agatatcttt
    44641 aacggacagt tccgactgat ttgcttcgag taaatcagtc gcttcctcta acttccagtt
    44701 gaggatcaat tgcataattt gggttgagag gatattttcc gccattcttc gcttttacag
    44761 gtgcattacg atgttatttg atcgatgtct cgattttttc acggggaatc caaacggaaa
    44821 tagtcccgtt acagaatgac tccgccgtcg caccgcagtt tgtggcaggc gcggtcaaaa
    44881 caatttcggt ctgtgatata aaaacaggcg gtgtaccgga cggggtttcc gaatattgac
    44941 gaatgtttgt ttggcgtgcc acattttgca tggtgctggc ggatatttgg ctctttccga
    45001 tattaaacgg gatttccaga ttgaccattt tatccgtata tccgatcagt tttccgggaa
    45061 cattttgaac atagttgatg accactccca gctttttctc cggagtcatt aattcatccc
    45121 cgtaaagatc gacattcccg tcaagattaa acacaacatc cttaccgatt aaagatttca
    45181 aatgtaaatc ggccgggctt aacaatgcat cttccgtcaa ttgagcggcg ggaacagcct
    45241 cggtatcggt aatcaaagga gcgactgcga tcggcatttc tgtaggcgtg atcgctatgg
    45301 tggcagtggg cagcggcggt tgagtcggaa tcggtgtcgc ttgttcggcg gcgatatagg
    45361 tcttttggaa gttggacaaa ttcgcttcca atcgggcaac atttcgatcc aaattgaaat
    45421 tttttatcaa gaccactgtc aataaagtga cagataggac gatgacgaca accaataaca
    45481 ccaggttcaa tgttgtcagc cgttgtaaat cttttgaggc tttacgggat tcgtttgcca
    45541 gattttttgt ccgtgccagc tcatcctgaa tatcgtaaag ttgcatgttc ttttggcgaa
    45601 agcttgagat tttttgatga acataaggga tattattttg gtttcgtaaa tatttcccgg
    45661 tttcgatgtt accactggct gtcgacagat cattcagcag ggaattaatc aaggccagtg
    45721 cttttttatc ttcgccttca tcttgttgac gactcatttc tgtacgtaat cgctcataaa
    45781 atgccgggta ttcaatgtaa tgcaaatcga ttatttcttt ggcagcttca aaatcgccgt
    45841 tctcgatcaa agctttgaat tgctccatat tttcggtatc caattttttc cggagagctt
    45901 tgattctcgg cagcagatca cggaccggca gtttttggtt caattcgatg gaagcactgc
    45961 tcgctctcgc cagatcattt tgtagattga acatatgact gcggatgaat tcgccgattt
    46021 gtatcggatt attttttacg attaccctta tgtcggcgac ggcttgcggc aagacagcag
    46081 accagtccag gctgtttaat tgatcgtcgg gtaccacaaa tcggtcgggg ccgactttat
    46141 tgatccaatc ctccgtctca tctaacattt ttaagaagac aggttccaac tctttttcga
    46201 ccaataacga atcttcccct tttgttcccg acaacaattc attcaaatcg gcaaaaaagc
    46261 cgatatttct taattcggaa aaactgttcg ctcgggaaaa aacgtccaat aacggttgag
    46321 ttcgtttctt gatctcggtt gtatgatctt cgagattttc cattttagaa tcctccgtct
    46381 cttcggacaa agaatccggt tttatgactt tgttgttttg agaagagacc gggacagaat
    46441 tttctgaaat atgccgtgct tgcttgcgat aggcatcggc aaggaacgga tcgactacgc
    46501 gtaaattgga ggttgtaaaa gcagcctgga acatctcagg tgaaatctga ccgctttctg
    46561 attgagcaag gaaatcccaa acatagatta catcttgaat ctgcttgaga tgatcttgaa
    46621 gaatcggaat cagttcggga caactttttg ttcgggaaat tttttcggat atcagcttcg
    46681 ccaatttttg gaattcgctg tttaatgcgg ttgtttggtc atttaagaca aatcggatgc
    46741 agacatcaaa accttgctga ggaatgagat cgactttgcc ataccaaaat tgaacggttc
    46801 gaatgaccgg caacagccaa cctttataag aagcaagcga aatgaggcgc ggatccattg
    46861 cctttctaaa atctttgtcc atgaaggatt cgggatcttt ttggataaag ttttccgctt
    46921 cttttttaag tgattcataa gaaagaacgg ttgtttttgt ctcggtcatg agagaatcca
    46981 cggcttcatt caacgcccgt aaaatatcat cccaaatttc aacgcacttg ttttggacgc
    47041 gtacttcggt cagatttccg gccaacatat caccgatcag cagcgcatat ttttggttca
    47101 gaggaggatt atcggtattg aaggaaagtt gtcccaacaa acgaatcagg atcggattat
    47161 tttctttttg gatcggatat tcatattttc cttgattgat tacctccagc atttttcgac
    47221 cgtaaatcaa ttcgggaaga aaaaggcaaa acttacgaat atcatccagt aacagaaagc
    47281 tcgaagcatt ctgacgggag ttcccccaat cgatcaaggt gacggaaatt ttatcttccg
    47341 ttttcgacca aaacaaatgt tccaacggtt tgatatcgac gtaggcaatt ttattctttg
    47401 cgcaagtccg aaggattttc gcaaaatcga gagcgattgc tacccgatct ttcaatgaat
    47461 ccggcaattg tttttcgcag gggattcctt ttccggcggt ttgaagttgt acagtgaagc
    47521 ggttacgatc caacattcgc ataaattcga cgttgaactt atcaatgtcg tctgtcagaa
    47581 cgtcggggac cggcatatag ttcaaacgtt ttgcggcgtc cggttcggac aatatttcat
    47641 ttttgaataa taaatttact ttggttaatg ttgaaatttc atccttcgag ccttcagcga
    47701 tttcggaaac cttcaaaaag tatttttctt cggtttcctg attcgaaaac tcgaatgcgg
    47761 aagtaaaccc acccgaattt ttcacaccgg tataaacaag cgggatgact ttatcccgta
    47821 cgcgaactga atttacttcc gttatgattt tttctaagtt atcttgtttc atcgtattct
    47881 cctgaccgaa cggctcacgc aggtcagcgc tattttccga gtccggttga tttatcagcg
    47941 gtgcggttgc gaaatctttc aggaataaag aggctcccgg tccgcaagaa attgaaaggt
    48001 ttgctcctcc gtatccagaa tcagtgcggt gcgcggaaaa tctttgcctt cgactccggc
    48061 actgcccgga ttgattccga atcgttgtat gccgtttccg tattctattt tttgttccgg
    48121 gacggaggga gtaaattgtc gccatatgtt gacaaacgtt gaaacggaat atacaaaata
    48181 tcccggaata tgggtatgtc cgaacaagaa atgctgcgtt ttcagatttt ctcggttttc
    48241 aaaaagtccg ccggtcacaa tgttcaaatc cttcggattt aaataactct cacagggctc
    48301 ggcatgatat acctgcccgt catggcaagg aaaactgcat ccgtgaaaaa cagtgaactg
    48361 aggattttct tccggcaagc aaaccacttc ttttttcatt attttatcaa aaagccggat
    48421 tttcctttcc gtaatgattt gtcgcctttc ttctaattcc gctttgcact ttgcctgtgc
    48481 ttcgcttgtg ttatctcgtt ccggatgcaa caaccaatat tcccagttcc caaatacggc
    48541 actgatcgga taatcggcta atcgatccaa agcatccaaa aaaccaagat caaatttcgc
    48601 gtttccattc gattgatttg aatgcccaaa caaatctccc agcagccaaa tgtcatcgaa
    48661 tccggattcc atcgcttcgg cttttctcat aaaacggaaa agactctccc cgttttcatg
    48721 aatatccgac agtaccagat gttttttata catgtttatg agctaaaaaa tctcctaatc
    48781 cctcaaccaa agcctcaaca tccattttag tattgtaacc ttgaaccgaa attcgaacaa
    48841 acgtgcgtcc gttccagctg attatcggga cttcaacctg atatcgatca taaagaaagt
    48901 tcttcagaac caaaggatca ggtaggtaag gtatttctgt gaccgccagt tgggcataag
    48961 catcgtcatt cgggtaaatt aaaggtaatt gggtgatctg cccaatttct cttaacgttt
    49021 caaacaataa caaatgacaa gctctccgaa cttgatccca atgattggct tccatgaatc
    49081 ggatggcagc cgggacgctt aaggcggcgc agggactgtt tgtccccgtc cattgattga
    49141 aatcaatcag ggggttaccg accgcatttt ccagaaaagg tccatgtccc caactgataa
    49201 tcaagggtgc aactttttga atccactgtt cactgacgta caggaaagcc gagcctttcg
    49261 gagcacacat ccatttatga cagtttccgg tgtaaaaatc acattcaagc gaaagaagat
    49321 ccacaggcag ctgccccggc gcatgagctc cgtcgataca ggtgataatg ccttcttcac
    49381 gtgcttttct gcatatttca cgaatcggaa aagtcagggc ggtcggagaa gtcaaatggc
    49441 tgagatagat tacccgagtc cgaggagtta cctgttccca taggcaatct attaattctt
    49501 gatcgctgcc atacggaaga cgaatgtctg ctttttttag gatagccccg cttttttggc
    49561 agcaatctat ccaaacattt tcgcaggcac cgtattcctg attgcctgtg acaacctcat
    49621 cgcccggttt cagattgatg ctgcgggcca tgatgttgac gccgtaagtg gcattgggaa
    49681 tatatacaac agaatcggct gacgtgttca aatagttcgc taaaatgatt ctggcttctt
    49741 ttaataattc gtgatattcg cggcctaaaa ataagaccgg ttgtttttcc atccgcaacg
    49801 tccatgaaaa aaaggtctcg atgacttctt tcggacaagc tccgaaagat ccgtgattca
    49861 ggaaatgaat agcagggtcc agcaagaatt tttgttttaa atcaattgaa ttgtccataa
    49921 tgctccttcg ttctcagtga ataattatat gttaccgatc attcaaaaac gggaaagcaa
    49981 gtcggtaaag ggtaatccgg cagcaaacaa tcgatcgaat cgccttccga aattcgttaa
    50041 ccgccggcaa atcaacttcg agaattgact tttccgatgg ctatcccctt tcaagcaaaa
    50101 taactttgca attttaatgc tttttcctgt ggattttatt gttttataaa tttacaaatc
    50161 gacaacataa ctaagaaaaa acaaccatat caaaaaagat cgagccggtt acaaatttac
    50221 tcatccgaag tccggatttc aaaaggcttc catttttata atttgtcaca ctgaagatga
    50281 actgttaaat cgatcattaa cgataaaata ttactgttgc ccgaacaatc ggcttcgatc
    50341 gtattatttc gaacgaaaga gcggaaattt tatgaggttt tatcgatttc ttatagtctg
    50401 tatattgggt ttcacttttt tgataaccgg ttgtgaatct gctcaatcga ctctcccgac
    50461 tgaaacttta atccgggaag agatcgaacc tttgttgctt ccggaaagta tgagagaacc
    50521 aatcgaggat tcggaaaaag ggatccttta ttggcaaaaa acgctctatt tatccgatca
    50581 tccggaaaaa tatatcgggg aaccggttga agtactggga tttatctacc gccgggaaga
    50641 ggatccgaag gaagtatttt tcgtgggaag attattgtta tcatgctgtt atgatgatgc
    50701 gctgccccag ggattgggtg tctatctgcc ggataatgaa aaatgggaga atgatcaatg
    50761 ggtaacggtc aaagggcatt gggatcgtat tcgcttcgga gaaaacagtc gctatttgat
    50821 tattcccgaa tctatttccg aagccgaacc gcctgaagat ccttatattt acgataaaac
    50881 agaaccggcg gaaacaaatt cggcaaatga aaatatctaa acgaaaaatt cataaaacaa
    50941 agatcgcttc cgcggaagcg atctttgctt gtgacgattc gattgaaaag actattctct
    51001 ggctcctaat ttctcatcca agatcatcaa accgcccgga ttatctccgc ttttctctaa
    51061 cattgcgata atatattcaa aattggcttc ttcttccacc tgttcggaaa tgtaccaacg
    51121 taacaattcc tgtgtcccat aatctttttc ttccaaagcc agatcgtaaa gtccgtgaat
    51181 gctggaagtg atgaactttt cgtgttccaa gccggcttta aagacatcca atggattttt
    51241 ataatcgacg gaaacttttt cgattgtgtc taaggaaact tcgccggatc gctcataaac
    51301 atagcggaaa aatttcatcg catgactgat ttcttccttc acttgcttat tcatccaggc
    51361 ggacattcct ttgtaattct cattttcaaa gtatgcttcc attccgagat aaatataggc
    51421 ggaatgaaat tctttattaa tctgttccac taaagcttgt tgcatctgtt tcgataacat
    51481 attttttctc ctcagttaat tgttgtttct ttgtcttccg attctttctg ccgggaatca
    51541 agaagaaata aaataaactc attacaactt agtaataatt ataacttaaa ttaagccaaa
    51601 tgcaagagaa tcggtaaaga aagttgctga tgaacgggac cctccttacg tgactaaagc
    51661 aaatattgtg tcgcaaaaga ataaagtgtc gaaagataat ccgtaattgt ctcagggcga
    51721 cggaatccgt gtccttcttc tttatacagc cgataaacat gcggacattt tagacgcgcc
    51781 gctaattcgt tcgattgaga aggaattacc accggatcgg catccccctg gaaaatcgca
    51841 aggggtgttt tgattttttc gacgtgccga atgggtgaac gttcttcata aagcggtgcg
    51901 gcttccggaa gcggtcccac cagcgaatcg gtataatgca attccaattt atgagtatta
    51961 atggctaaac cgaaaaggtc ggtcacccca taaaggcaag cagccgcttt aaaagaatcc
    52021 ggatattgaa ttaatgtatt gagaacggta aaaccgccgg cgcttccgcc aatgatcaag
    52081 agccggtttc gatccgccaa gtgtagtgca cttaagcagc tcgcaccgag aacggcatct
    52141 tccacatcat agacgcccca attgccgtac aaacttttca gataaccggt tccataaccg
    52201 cagctgccgc ggtagtttac ttcaagatat ccgaatccgc gggaagtgaa aaattgtatt
    52261 tccgggttga agcgtaaatc cgctttgccg gtcggtccgc cgtgaatatg aacaaaagca
    52321 ggcggaagtc ccacccaatg tgcatcgggg ttggttggcg gataaaagat accgtaaacc
    52381 ggagtcccat ccggtgcttc ccatgaaatc tcccgcggtt ttgaaatata ttcttcgtct
    52441 atcagatcat cactcaatcg aaagacgatt ttgaatccgt cgtcatttac cagaataatt
    52501 tgagtgggtt gtaacgggct gtcggctacg gctgcgatca ttccggtctc aggagaaacc
    52561 gaaatgacgt caaaagaaga atattgcgga gaatttaata ttcggatttc tccggattca
    52621 accgagacga tacaaatttt gcttctggtc ccaaaaattt gtaaaaatgc gatctgccgg
    52681 ctgtccggaa accagtcata agtctgcact ccctgggcaa aagccggaac cgtcaaagca
    52741 aagtgatctc cgtggaccaa gattttttcc tctccggatt ccaaatttcg caggatcaga
    52801 tcttcccatt caccattcga tcgtatgtaa gataacattt tcccatcggg tgagaatttt
    52861 ggctgagaag cggggaacgg cattttaccg gcaatatgca cgatttcgga gattgttccg
    52921 ttttccggat cgacttttcc gatcatcact tttgaaccgt cccacggcat gtccggatga
    52981 tcccattcca cccacgtaaa ataatctccg cgtggactcc aagccggttg catatagaaa
    53041 tctgctccgt gaacccataa aaacggccaa cttcgttcgt ccaaacgcag caatgccatt
    53101 tgatcatccg taccgtcgcc ggatatataa gcaacaaatc gaccgttcgg actgattacc
    53161 ggagaagcgg tcatataccg gtcattggta agcaacttga atgccggttt atttaggttc
    53221 tgaagaaata atccgcgttt gccggcgctg aataatactt tttcgtttcg tacatcaaaa
    53281 tctccgccgc cataaccgat atttccccct accggtaaat cgccgttcag cttttttaac
    53341 cccgaatcac agcgatattc ataaagaaat gaatttcctc cccgattccc ggagaaatag
    53401 agacggttgt ccgatcccca ttttaaggta ttaatcctac cgcgttgagc gactgcctgc
    53461 tcagaaatgg gacttttcca aagtccgtaa ggaattggtc tcataggttc cttctgtttc
    53521 agaatgattt ttcgaaaaaa gatccttttc aaaaattttg ataaagaaat cataaccgtt
    53581 cagccgttga ttttcaatgg ctttgacaaa atcgggttat taatgattct tataaaaaaa
    53641 ccggcaggat tcggctgagg aaaaaagatc ggcggcactt gccacagcgg gggcacaccg
    53701 aagcgataat cgggaacaaa aaacatgttt caggcgttca gcccgaaaca tgtttttgat
    53761 tttcattgtc ttgttgatcc gccggagaaa tccgatccct atactccggc gaaagaacaa
    53821 atgattgatg acaaaaaaat tatgaagccg gttttacttc aacggttgcg ccggccgctt
    53881 cgagtttgct ctttgcgtca tttgccgctt ctttaccgac agcggaaagg actttcgcgc
    53941 cggcggtttc agccatttgt ttcgcttcga ctaagccgag atttgtgaga acacggataa
    54001 ccttaatcgt ctcgatcttc ttcgggcccg catctttaat aatgacatca aactcggttg
    54061 cttcttcagc cggtgcagca gcttcggcta ccggtgcagc cgctacggtt gctacaggcg
    54121 ctgcagcgga aacaccccat ttgtcttcta acgctttaac taattctgaa gcttctaaaa
    54181 cacttaaaga gcttagcgct tcaacaagtt tttcaatatc agccatttta aaactcctaa
    54241 ttttattatt tatattttta tttatttcaa tacgatcagg cggcagtcgg ttcctctttg
    54301 gaaatgtgtg cctgaatcac tgcggcgagt gaacgcccgg gttccgcgat aacacgagtc
    54361 aattgtgaag cgggtgccaa aatggtaccg agcaattttg cccgcataac cggcagcggc
    54421 ggtaaactcg cgagagcttt tacctgactt tcggaaattt cattcttatc taaaaagccg
    54481 ccttttattt taaaggcttc attctttacg agatccgaaa agacttttgc cattgcagcg
    54541 gcatcggtaa aagcaaaccc gactaatgaa gttccggtta tatctttctg taacttgtaa
    54601 ttctcctttt ccagcacttt ctgaatcagg gtgtctttta caacatgaac ttctccgccg
    54661 gcttcgcgtg cttttgcgcg aatttcatca atcgccggca tatccatctt tgaatattca
    54721 agcataaata ctccctgact tttttgaagc cattgggaat attgctccat cattttttgc
    54781 ttttctgatt tagtgaatgc caatgaataa tacctcctcc ctttaatttt tcaaaaaaaa
    54841 agtctttgcc ccgaaactcg aaggcaaaga cataaaagtc ataaactcta taactgtcat
    54901 tcgcctcggc aggatattaa gcctttcggc accggctgtc ttcagagaac tttttttatt
    54961 ttacctgatt aatttgctct ttacttatta ttccttacaa attaagcgat tggttccaat
    55021 ccctgagccg cagaaagatc cacatgaatg ccgggtccca ttgtcgaagc caaagtaatc
    55081 ttcttgatat aagttccttt tgctccggcc ggtctcgctt ttcgtacggc atccaagaat
    55141 gcagccaaat tctcgtaaag tgctttttga tcaaaagaaa cttttccgat gggggcgtga
    55201 atatttcccg acttgtccag acggaattcc acacgaccgg ctttcgcttc tttgatgaca
    55261 cgcggtaaat cttcggccgg aacgacggta ccggctttcg gatttggcat taaaccgcgc
    55321 ggtcccaaga cacgacctaa acgacccgct ttcgccatca tgtccggagt tgcgatcgca
    55381 acatcaaaat cggtgaaccc ctgctgaatt ttctggatcc attcatcgct gtccgcgata
    55441 atatctgctc caccttcttc ggcttttgcg gcgccttctc cctgtgcaaa gacaaggacg
    55501 cgtacggtct tgcctaaccc gttgggaaga accacaacat cccgtacctg ttgatccgcc
    55561 tgacgcggat ccaaaccggt ccgtaagtgg atctcaacgg ttgcatcaaa tttcgtgatg
    55621 ctggtttctt tcaccaaatc aatcgcatct ttcggcgcgt aattggttga tggatttact
    55681 ttcttctgtg cttcctgaaa tttctttccg tgttttgcca ttataactcc ttgtggtccg
    55741 atcgagtatc tgtcgtactc tcccacgaaa tattaatcaa ctacctgaat tcccatattc
    55801 cgagcggtgc cttcaatctg tttcatggca ccttcaatat ccaccgcatt catatccttc
    55861 aatttcgttt cggcgatttc ccgaacctga gcacgggtaa tttttccgac ccgacctttg
    55921 ctgccttccg gagaagcggt ttcgattccc gctgcccgtt taatcatata ggaagccgga
    55981 ggtgtcttta gaacaaaagt gaaagaaccg tctgtataaa cggaaatttc tgccgggaca
    56041 atctcaccga tgcgggtttg ggtccgggca ttatattctt tcacgaacgc catgatgttg
    56101 atgccatggc ctgcgagggc ggggcctacc ggaggtgccg gattggcttt gcctgcattc
    56161 aaatttaatt taacaattgc ttttaatttt tttgccacaa tatactcctt cgtggtgcta
    56221 acggatttta tccttccaca aaaataaggg acccggtaag gaccgttttt tgtccctctt
    56281 tatttcctca ataaattctt acgctttttc cacctgcaga aaatccaact ccacaggggt
    56341 ttccctgccg aagaaattga ccattacccg gacttttgat ttatcctggt caatttccgc
    56401 aaccgtcccg cgaaaatcat tgaaaggtcc gtcaataatc cggacttttt ctccgacttt
    56461 ataggttacg ctgaccaccg gtgcttcggc ttccatccgt ttaataatcg aattcacttc
    56521 ttccggacgt aagggagtcg gttggtttcc cattccgaca aatccggtaa cgcccggtgt
    56581 atttcgaacg ataaaccatg attcttcggt cagaatcatg ttgaccaaga cataacccgg
    56641 gaaaatatga cgttcaatcg tcttgcgttt cccctctttg acttcgattt cttcctgagt
    56701 cgggataacc acatcaaaaa ttttatcctt catccccatg gaatcgatcc gctgttcgag
    56761 gttatgacgg actttgtttt cataaccgga atagcaatga atcacatacc aattccgttc
    56821 ttcattgctt tcgatcttgg tttcttcttc gggtatttgt gcagatggtc gttctgtttt
    56881 cttggctttg gcgcgagtcg cttttttcgc tttcaatatc tcttccgcgc cgatatcttc
    56941 atctgagtta ttcagaacat ccaactcatc tttctcttta gagctcattc aacaatcctg
    57001 ttccttatcc gacgatcgtg ccaattagtt cagagaacaa aaagtcaaca attcccaaaa
    57061 aaaccgccat cgcaaaaaca acaatcaaaa cgatttttgt catatgaagt gcttcttctc
    57121 tggtaggcca ggttactttt cgtaactcac caatcgtctc attccaccat ttagcgattt
    57181 tattcggctt cttcggagcg gatggttttg ccggtttttc tgttttcttt atttttgtgt
    57241 ctgccataaa atggtcactc cttatttctt cggcacttat gcctataaac gccgcttgaa
    57301 tgcggcgtca ccttcttgca ggtgaagcag gaatcgaacc cacaacctcc tgttttggag
    57361 acaggcgctc tgcctaattg agctattcac ctaagatacc cgaccgataa gatacgatcg
    57421 gtcgggtatt tctttttctt attttgcttc ccgatgcaaa gtatgttttc ggcaacgcga
    57481 acaatacttc attaattcca ttctcccggg atcattgcga cgattcttct gtgaggtata
    57541 gtttcgctct ttgcattctg tgcatgcaaa cgtaatgacg gggcgaacat ctttcttttt
    57601 ggatgccata ttctttctct ttcagcgtca aacgctgata ctccttctta tccttcgatt
    57661 tcagtaatca caccggcgcc gacggtcaac ccgccttcgc ga
//
LOCUS       DAOSMM010000006        52041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_692_length_52041_cov_17.568922, whole genome shotgun sequence.
ACCESSION   DAOSMM010000006 DAOSMM010000000
VERSION     DAOSMM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 52041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52041
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_52041_cov_17.568922"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1215)
                     /locus_tag="PK898_02015"
     CDS             complement(1..1215)
                     /locus_tag="PK898_02015"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOU18839.1"
                     /translation="MSSNEWEKFKLSEIMEIIGGGTPRTNIVEYWNGNIPWLSVVDFG
                     GEKKYVFETEKSITQLGLDNSSAKILKKGQIIISARGTVGELAILGKDMAFNQSCYGL
                     NSKAMTSNDFLYYLLKYNIEQIKRKTHGAVFDTITRETFDHIEINLPPISTQRRIADI
                     LSALDDKIELNRQTNATLEAIAQAIFKEWFVDFNFPGATGELVESELGMIPKGWRIGS
                     VAEILILANETVSPGSNPDKLFYHYSIPAFDSGRVPSQELGSAILSNKYKVKSNSILV
                     SKLNPRIPRVWPLKEVNQESSVCSTEFQVLLPQKAHYYSFAANLFSQTSMIDTMKSRA
                     SGTSSSHQRIIPQDILDINIIIPNDDLLALFDEIVGNYYHCIINGLEESVILAKIRDS
                     LLPKLMNGEIEV"
     gene            complement(1423..2508)
                     /locus_tag="PK898_02020"
     CDS             complement(1423..2508)
                     /locus_tag="PK898_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOU18840.1"
                     /translation="MISDLYAFPPEKVAQSDTLNRQIVMAHRRLAELKGVAPLLPNQD
                     ILLNTLALQEAKDSSAIENIITSHDELFKQELDIPQFNNAAAKEVGRYSEALKLGFSR
                     IETKGELSLEDLVAIQAVLVIGEPGPGIRSRPGTIVGNSATRKVIYTPPQDHDQIVEL
                     LERLLNFFNEAVDEGLDPLVRMALIHYQFEKIHPFYDGNGRTGRILNLLYLVQHGLLD
                     LPVLYLSRYIIREKNAYLTQLRTDPLDDNWEAWLAFMLRGVELTSMETIETIHQLRDL
                     MADTQEKIKMQLPKIYSLDLLNTLFKHPYTKIDFVIRDVGVKRVTAGSYLDALAEAGF
                     LEKQKIWRSNYYINVPLFKLLQGNYES"
     gene            complement(2498..4024)
                     /locus_tag="PK898_02025"
     CDS             complement(2498..4024)
                     /locus_tag="PK898_02025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOU18841.1"
                     /translation="MTENNHELEKTLWAAADKLRSNMDAAEYKHVVLGLIFLKYISDA
                     FEDLYQKLTENQGEYEGSDPEDPDEYLANNVFFVPEKARWQYLQDRAKQPEIGKYLDD
                     AMDAIERINPPLKGVLPKIYADPELNKQRLGELIDLIGTIGFHQDAHTSQDLLGRVYE
                     YFLGQFADAEGKKGGQFYTPASIVKLLVEMIEPFNGRVYDGCCGSGGMFVQSEKFVRN
                     HQGSIKDLSIFGQESNPTTLRLARMNLAIRGIDAKLELGDTFLNDRHPDLKADFILAN
                     PPFNVSDWSGEQLRDDIRWKYGVPPVGNANYAWLQHFAHKLSPTGIAGIVLANGSMNS
                     NTGGEGEIRKNMIEDGLVDCMVALPSQLFYNTMIPACLWFLARDRTNHKFRNRSNEIL
                     FIDARKLGTMINRRNKEFTDEDIAKIAGTYHAWRGESGVYEDIPGFCKAATLDEVRAN
                     NYVLMPGRYVGTEEAEDDGIPFEEKMNTLTAQLAEQFARGNELQQRIRENLKGIGYDF
                     "
     gene            4358..6439
                     /locus_tag="PK898_02030"
     CDS             4358..6439
                     /locus_tag="PK898_02030"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOU18842.1"
                     /translation="MDDFSYNSQYLIRNGKPWFPLMGEMHYSRVDRRFWADSLQKMKA
                     GGIDIVSSYVIWIHHEEIEGEFDFSGNKDLRKFIQLCQDHGLYFFLRIGPWVHGEVRH
                     GGFPDWLLAKGFEVRTNDERYFAEVYRFYDRIYSQIKGLQLKEGGPIIGIQIENEYGH
                     CGGLTGPEGEAHMRRLTAMAKEIGFDVPYFTATGWGGAVTAGLLPVMGGYPDAPWDSR
                     LTEIEPSGNYIFTPERNDHNIGSDHHIGYGLTFDPAEFPYLTAELGGGLQVTYHRRPV
                     VTAADIGAMTLVKLGSGVNLLGYYMFRGGSNPPGKRSSLQESRAAGDFCDLPQISYDF
                     RAPIREFGQISDSFREIKRFALFLHDFGEELCKMPAKFPSSNPLDPKNNRDLRTAVRD
                     NGKNGYLFVNNYQRRQKMADHPQTSLRVTLPDETIEFPITDIRDGDYFFFPFNLPIGN
                     SILKSAAATPLCKINDDTTVFYSDFPPQYQFDHEPLNHKIITLSKKQAMNAWKIRLPD
                     GDHLLLSEDVVLQNRDRLEMIGRGIAELSIYPDFPKPPHNAVRIGGEGDFTVYEVRHP
                     GFDPNRNPAEAKFRIVRQTPEQTDYEITLNYPTDVENCYLSVGYQGDQARLIVDGKLS
                     ADDFYAGTEWEIGLKQFNFPKKIILSVTPLYAGMPVYLEQWPIIEGEKICRLNHISLN
                     CEYRTIINRIR"
     gene            6663..7382
                     /locus_tag="PK898_02035"
     CDS             6663..7382
                     /locus_tag="PK898_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOU18843.1"
                     /translation="MEKNHFSREEAKERIIGASVKLFSEKGFDGTRVNEIAEAADVNK
                     ALIYYYFDNKEAILDQLLNQILNNVTSLSTTFVQKSIRQMIQDGRLCIMKDRLHFANE
                     AALENFLSHIQNFNNQILDYAMNNRKLIRILMTESLKESKHKNDLIQFIKELGKAAEN
                     PIIRTIREANADFPLSNNFIFLRFFFHPVSIINFAVYYETYQKICGLSESKMRTAMLN
                     SIRTLSNSFVSGNDIILQTEA"
     gene            7472..8851
                     /locus_tag="PK898_02040"
     CDS             7472..8851
                     /locus_tag="PK898_02040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU18844.1"
                     /translation="MKNIQDSIEELIIHYVVTDPLGKLPKMLNLAEKIDQGHLHEKEI
                     NGIRKILDDKNGNWYKFIEGVSEDIDPKCLQKVIECFMINASLKGEAKARAVEKKYDM
                     NVPWTILVDPTSACNLHCTGCWAAEYGNKNNLSYETLDSLVNQGVDLGIYFYIFSGGE
                     PLIRKDDIIRLCKAHQDCYFFSFTNGTLVDDQFCKQMREVGNFAVAFSIEGNEEATDM
                     RRGKGTYRKVIAAMDLMKKNKLLFGYSTCYHRYNTESVGSDEFVDDMIARGCKFAWNF
                     TYIPVGKDAVPDLLATPEQRKYMYHRVREIRATKPLFAMDFWNDGDSTNGCIAGGRRY
                     LHINAAGDVEPCAFIHYSNANIHDMSLLDALKSPIFAEYRKGQPFNGNHLRPCPLLDN
                     PQALARMVKASGAKSTDMEAPEDVDVLTAKTKAAAEKWAPVADELWEKQNKAKAEKQA
                     EAQKVEIEA"
     gene            9106..9843
                     /locus_tag="PK898_02045"
     CDS             9106..9843
                     /locus_tag="PK898_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOU18845.1"
                     /translation="MVTRVIESEDSKDRIIKAAVQLFSEKGFDGTRVSDIADLADVNK
                     ALIYYYFTGKEDILDILLRQLLNDITAFSMDFIHTHLISMIRDGTLELKEDRLTFIDK
                     NAVDSFLDSMRGYYRKAVSYALENRQIIRILMLESLKNSKHQNDLYLFLKLLNKNDNN
                     PVYKTILNADWNFNYSDEFILYKFFFSMMPIINFAVYFDDYKNHSDMEEEDLINRFLQ
                     SCQTTINALVCGNEILMRNDCSIIESR"
     gene            9950..11320
                     /locus_tag="PK898_02050"
     CDS             9950..11320
                     /locus_tag="PK898_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU18846.1"
                     /translation="MNSKVQNAMLSLLMGYLENDPMNKIPAILDLAEKMDTGHVHANS
                     FKVVRRELTDKNGVWYQFTENLFNEVDPRVIKKAVTSFFFNGILNNGTKQDKLREKYQ
                     CNIPWAILMDPTSACNLHCTGCWAAEYGNRNNLSFETLDSICKQGKELGIYFYLLSGG
                     EPTVRKEDVIKLCEANPDCFFFAFTNGTLVDDKFCDEMLRVGNITLGFSIEGDEAATD
                     MRRGKGTYQKVIAAMDRMKKHKLLFGYSTCYHRYNTESVGSDEFVDDMIAHGCRFAWN
                     FTYIPVGKDAVTDLLATPEQREYMYRRIREIRATKPIFALDFWNDGEYVGGCIAGGRR
                     YLHINAAGDVEPCAFIHYSNVNIHDVTLLEALQSPLFMAYRQGQPFNENLIRPCPLLD
                     NPQALSKMVKVSNAKSTDMEAPEDVDVLTSKTKPAAEKWAPVADELWEKHLKEKEEKE
                     KEKIAG"
     gene            11481..12848
                     /locus_tag="PK898_02055"
     CDS             11481..12848
                     /locus_tag="PK898_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOU18847.1"
                     /translation="MDEIKTIVDTQRRFFLSGKTLPVSQRKKALQSLLTMVDENQQMI
                     FDALFTDLHKSEFESYMTEVGLVLTEIRQAMKNLDAWTSVKKVRTPLYLFPSKSSVYP
                     EPYGTVLIMAPWNYPFQLCLVPLIGALAAGNTAVLKPSAYAPAVSRCIAELIHRYFPS
                     EYIAVTEGGREENSSLLEQHFDLIFFTGGSRMGKIVMESAAKNLTPVILELGGKSPCI
                     VDQTADLKNASRKIVFGKLLNAGQTCVAPDYLLVHESVKDTLIHNLIEEFDKAFPDKD
                     YSDFPAIINEKHFDRLNRLIRDENVIYGGNSNRDRRFFEPTILFPVQWETPVMQEEIF
                     GPILPVLTYENLDDVIDRLRKLPKPLALYLFTEDRKTEKKIIENVAFGGGCMNDTVMH
                     LSNPNLGFGGIGESGMGQYHGKNSFDSFTHYKSMVKTPVWPDLPIRYHPYTKQNLNRL
                     KRFLK"
     gene            12968..14347
                     /gene="amrA"
                     /locus_tag="PK898_02060"
     CDS             12968..14347
                     /gene="amrA"
                     /locus_tag="PK898_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOU18848.1"
                     /translation="MSIIAAYIVPHPPIILPEIGKGEERKVQKTIDSYKKIAAEIAES
                     EPETIVITSPHATLYADYFHISPGRHAAGDFGQFGARGLVISADYDEEFVQELEKTAE
                     ENNIPAGTAGERDPRLDHGTMIPLRFIQKAWDPNKKPFKIVRIGLSGESLLTHYEFGK
                     CIAEVAEKLNRRTVFVASGDLSHRLKEDGPYGFVKEGPIFDQQITNIMRSGNFSEIFN
                     LDPHLCDQAGECGLRSFVIMAGALDGKQIEPELLSYEGTFGVGYAVASFKIKGIDENS
                     RFNRYETEFQINNGEDPYVALAKASLTTYLKTGKYMKVPSGLPAEMLNRRAGTFVSLK
                     KNGELRGCIGTILPVRSSVAEEIIHNAVSAGVEDPRFPPVTLNELDDLDFSVDVLSEP
                     EDISSPDQLDVKRYGVIVSSGYRRGLLLPDLEGVDTVEEQIDIARRKAGIPRGEPIRL
                     QRFEVVRHT"
     gene            14344..15168
                     /gene="amrS"
                     /locus_tag="PK898_02065"
     CDS             14344..15168
                     /gene="amrS"
                     /locus_tag="PK898_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU18849.1"
                     /translation="MKATCEICFHHCSLAEGQIGFCGARKNVDGKVICDNYGKITAIA
                     LDPIEKKPLKHFFPGSYILSIGSYGCNLHCPFCQNSDISMQYAGIRTHFISPQELVSK
                     ALELKSDGNIGVAYTYNEPFISYEYVTDCAGLVHQNGMKNVMVTNGTVEEAPLRKILP
                     LIDAMNIDLKGFTGEYYRKLSGNLEVVKRTIEIAAKECHIEVTTLIVPGENDSDEEME
                     ALAGWLASVDPEIPYHVSRFFPHYHMPDKPPTPVKTVYHLAEIARKHLKYVYTGNC"
     gene            complement(15241..15732)
                     /locus_tag="PK898_02070"
     CDS             complement(15241..15732)
                     /locus_tag="PK898_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU18850.1"
                     /translation="MESKTMQDLMAGFAGESQANRKYTAYARKAKAEGKNNAAKLFQA
                     AADAETIHALRHFEVAGKVGSTADNLKDAIAGETYEYTEMYPEFLKDAEAEGNKAAVG
                     TFTLAKKAEEVHAKLYKEVLDNLDETEEVFYYLCPVCGNIEKVRPGKCPICGADGSQF
                     IKY"
     gene            complement(15743..16192)
                     /locus_tag="PK898_02075"
     CDS             complement(15743..16192)
                     /locus_tag="PK898_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOU18851.1"
                     /translation="METEPAYRDLVKELKEKKIRLSYQRVKILDYLNRNRTHPTADEI
                     YTGLIAEIPSLSKTTVYNSLNSLVKANLVQELTIEDNENRYDIVTETHGHFKCEKCGR
                     IYDFTVGLDSFNPEELKGFQINQKGVYFKGICKKCLETNQKIQQNEE"
     gene            16827..17837
                     /locus_tag="PK898_02080"
     CDS             16827..17837
                     /locus_tag="PK898_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU18852.1"
                     /translation="MKKKIQIVIPMAGYGSRLRPLTWSKPKPLINLAGRTSLDYLLDE
                     FTSLENEFDPEYIFIISPNGWQIKAYMDKHYPNLKCNYVVQEEMKGQSHAIFLAKDLI
                     SGPMIMTFSDTLLEGDLGFLADEDADGIAWVQWNPEPQRFGVAITDENNRIERFIEKP
                     STDEYKMVIIGFYYFKEGQKLIEAIEQQIKENISLKNEYYIADAINIMIQKGAKFTIH
                     ETKLWLDTGVPETVFSTNRHFLDHGHDNTETAMKRKGVAIVPPVFIAEDATVESSVIG
                     PYVSIASGCHIQNSVIENSIIAENTTITGEVIKDSLIGEKVSLKSTPAAYFIGDNSII
                     EK"
     gene            17890..19038
                     /locus_tag="PK898_02085"
     CDS             17890..19038
                     /locus_tag="PK898_02085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU18853.1"
                     /translation="MYNNLSNRAKTIKPSAIRKYFAVPDDVISLGIGEPDFDSPACVI
                     EAGVHALQSGKTHYTANSGLTELREAISDDLYRRYRVRYDPNSEIIATVGCSEALFLA
                     LAALIDPGDEVIVITPCFVAYQASVLLASGVVVEVPCKFENNFDLDIAAVEAAITPKT
                     KAILLGFPCNPTGAVASREELQKLCDIAVAHNLAIVSDELYDQLVYGIEHVCVPSLNG
                     GFERTMLVGGFSKNYAMTGFRIGYLVAPAPLMDGAYKIHQYLIMSAPTIAQYGALAAI
                     ENGQEDAKRMHDEYDRRRHLVVDGLRSIGLPTVEPKGAFYVFPQISGTGLSSEDFANR
                     LLEKEKVAVIPGNGFGAGGEGFVRISYATSYEKIEQALERMGRFVKSL"
     gene            19210..20175
                     /locus_tag="PK898_02090"
     CDS             19210..20175
                     /locus_tag="PK898_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18854.1"
                     /translation="MYGYEYTNHLILNEQMVNLWQEEDGKKVAEVWVLPGYGANLCRF
                     KVDNIDYLYPAPQKLVDIRHYGTPVLYPTPGIVRGEKFTFDGSLYRFPANRGSIFRHG
                     FAMGQKFLFSEPIVSEDGVTLKTSLGIFRDHPLYYLFPIANRLDIDFTLKNRSVRIDI
                     TVTDLDTQKRFPFGFGLHPYFNLIGPREENLIQVPVKKWLDQAENQLIDPNDSPIDFR
                     SPTTVPETAIDEVWTGLTSEASPSIIYQSVGKRLVLHATDDFTHFVTYAPENAPYVCL
                     ENWTCSPDAHNLYAGEKNDVAHLLILNPGEVFTGSVQFSIEPLNN"
     gene            complement(20230..21555)
                     /locus_tag="PK898_02095"
     CDS             complement(20230..21555)
                     /locus_tag="PK898_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU18855.1"
                     /translation="MTEGIIWKQLLFFAIPLFLSSILQQFYGIIDSLFVGNFVGDLAL
                     AAVGSTGSLVNLLVGFFLGLSAGAGVVIANSYGAGDAVRMEKAKHTAILVAAICGVAL
                     TLLGWILTPVLLRFMNTPDGVYELAIVYLRLYFLGMIPMLIYNVGAGIMRSIGNSREP
                     MMILLFCGIFHIFADYLFITLWKMGVAGAAYATGISQLLSAVWVMWRLVRSDGPDRLE
                     WQRMKIDMPVLGSIIRVSVPAGFQSSMYSIANLMIQSYINMFGSVAMAGSAAYFKIDN
                     FVFSPVTAMGLAVTTFVSQNVGAGRFDRIQRAIRNALMMAFSFSIFLAVIVILFRYPI
                     LGWFTKSPAVIETGILFIFYLIPFYPIHNLTEVLSGTIRGAGNATTPMVITIFCIGIF
                     RIVWVLIMTHFWFDLRMVIVSFPISWILCAACFCLYYKKNATLITRKAL"
     gene            complement(22223..22978)
                     /gene="nagB"
                     /locus_tag="PK898_02100"
     CDS             complement(22223..22978)
                     /gene="nagB"
                     /locus_tag="PK898_02100"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU18856.1"
                     /translation="MKIKFIPAKDAVEAGRLGADIFTQTMEENPACVFGLATGSTPLS
                     LYSELVLRYSQGKLDFSLVRTVNLDEYIGLPADHPQSYHQFMYDHLFSKINIHPRNTH
                     LPNGMADDLESACREYEKMIESMGGIDLQLLGIGLNGHIGFNEPSDYFPKYTHVVDLT
                     ESTREANKRFFTDIREVPTQAITMGIGTIMSARKILLLCTGQKKAEILHEAMFGKVRP
                     QVPASILQFHPKVIVIGDEEALSVIRRDHPDFL"
     gene            23143..23739
                     /locus_tag="PK898_02105"
     CDS             23143..23739
                     /locus_tag="PK898_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU18857.1"
                     /translation="MKDLLNSIRERRSIYSIAKKSPISDERIRELVSLAVMYTPSAFN
                     MQSQRAVLLLGNHHEKLWSITKDTLRGIVPPEQFKSTEKKIDGFAAGYGSILFFDDTE
                     TVADFQHKFPLYADNFSVWTEHSNGMLQYIAWTLLEAEGFGASLQHYNPLIDEKVKSA
                     WDIPASWKLIAQMPFGLPTAPADKKEFVPIEKRFKAFE"
     gene            23856..24683
                     /locus_tag="PK898_02110"
     CDS             23856..24683
                     /locus_tag="PK898_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOU18858.1"
                     /translation="MKTNIEIIHPLPPETYIRHALFDFNGTFSLIREGWQEIMIPMMV
                     DLLMQTPEHEEEAEVTRVVKEYVTQLTGKQTIYLMMQLAEEINKRGGTAESPDVYKDI
                     YQTKLLERIQYRREGLRNGNIRPEEYLIPGSVEILQALKSRGVKCYLASEMDEKYVLE
                     DADLLQLTPYFDEIHGTLDDYVKVSKKMIIKEIILDNNLTGPEFAGTGDGFVEIENTK
                     AVNGIAIGVAPDEKDHTKVDPWKRERLIQAGADLIIPNFTDTGGLIDLLFQGKSKIF"
     gene            complement(24751..25728)
                     /locus_tag="PK898_02115"
     CDS             complement(24751..25728)
                     /locus_tag="PK898_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOU18859.1"
                     /translation="MDKKEAYFGHPSQISGVEEHRLVGGKGDGIRLFQVKNGLGLEFT
                     VSADRGADISRLSFQGLNYGYFPVGGYCSPAYYDDRGNGWIKTTNLGFLATCGLTNIG
                     IPSEDDGEILGQHGRAHNTPAEHIWWEEDQDEIQICARLNHSMFFGEKLIQNRQIVCS
                     KNENRITLTDRIENIGDKISPLMILYHINIGYPLLDEDLIVDIPSTTVTPRDEHANEG
                     LAEWNKMIPPQPGFVEMCYYHHFDQAKPVKVFQPKYQKGVAIYFDPASLDEFIEWKMM
                     GVKEYVLGLEPANSRLDGRAKNRRENRLKLIHPGQEIVYQVRFEMIESL"
     gene            complement(25752..26537)
                     /locus_tag="PK898_02120"
     CDS             complement(25752..26537)
                     /locus_tag="PK898_02120"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HOU18860.1"
                     /translation="MSLNNQQVENVEHILINCLRDKIRNYKPETISMPFHTRLLGKDR
                     MALFSFIQSLNTTFGISIFEPIAKSIAKDNFKVVQSQFTSGSLISSESQSVIQQIINQ
                     LENGERIPDKKAEIEEIRRVCQSGQFININPIKVDLFLSTFDDELFLIDLKTAKPNKN
                     DFRSYKRTLLNWVGVTLAQNPYAKVNTLIAIPYNPYEPKPYRRWTINGMIDLDNEIKV
                     ASEFWDFLGGEGTYEILLTCFEKVGIELSSELDDYFMRFENRH"
     gene            complement(26539..28125)
                     /locus_tag="PK898_02125"
     CDS             complement(26539..28125)
                     /locus_tag="PK898_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU18861.1"
                     /translation="MNNTELLTINEASDWATKYLKKKVSPSNISYLIQYGKVKKIFDG
                     ENTKIQKDDLKNYYDSFNGKREIDWKSKLGEDLNWALSFDHVKESETTKHVHRLHPYK
                     GKFIPQLVEYFLDDHVDKFKTEVVFRPGDIVLDPFCGSGTTLVEANELGINAIGIDIS
                     IFNSLISKCKIQKYDLPSVYSEIRRITNALKEFIYYSKIAEFEAALLEELNIFNSKYF
                     PSPEYKYQVLKRDINPDVYGHEKELIFLPKYYDLITKYQIDIEFPETGNFIDRWFFPS
                     VKKEIYFVYDEIKKINDQSTKEILSLILSRTMRSCRATTHSDLATLKEPQTTTYYCPK
                     HSKICKPLFSILKWWDTYTKDSIKRLAEFDTIRTDTMQVSLVGDSRNINIIDEVMKLN
                     PNFGKKISEQKIKGIFSSPPYVGMIDYHEQHAYAYDLFGFDRHDSLEIGPLYKGQGES
                     AKQSYVEGISEVLINCEKYLSNDYDIFLVANDKYDLYPQIVNKSGMKIIKRYKRPVLN
                     RTERDKGAYSEVIFHIKKDL"
     gene            28412..29233
                     /locus_tag="PK898_02130"
     CDS             28412..29233
                     /locus_tag="PK898_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU18862.1"
                     /translation="MKSEIEIIKPLPDKIDIHHALFDFDGTLSLIREGWQQIMIPMMV
                     DFIMQTPNHEDKAEVQRIVKEYVTRLTGKQTIFQMIQLAEEVEKRGGKALDPREYKEI
                     YHQKLLERIHDRLEGLRNGTFQPEEYLVPGSFSMLQALKARGVKCYLASGTDEKYVLD
                     EAQLLRLTPYFEGIYGAQDDYIRFSKRMVIQRIIRENKLSGPEFVGFGDGFVEIEDTK
                     SVDGTAVGVASNEQTREGIDEWKRERLIQAGADLIIPDFRCSDELIKILFPKESR"
     gene            29246..30226
                     /locus_tag="PK898_02135"
     CDS             29246..30226
                     /locus_tag="PK898_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18863.1"
                     /translation="MAYKTFDRSKLNIKPISERIHDLHLPQMLIPLDAPVPPFSDPAI
                     PKIAQAMIKAHENNAPVILMMGAHLLRKGMAPMIIDLMEKGLINHIGLNGAGCIHDYE
                     LARIGATTESVARYIHEGQFGLWQETGELNDIINHGSDAGLGIGEAVGKAIVENDYPY
                     RKTSLLARAWELGVPVTVHVSIGSDIIHEHPNCDGAKLGKASYQDFLVFTNTIDHLEQ
                     GVYLSYGTAIMGPEVYLKALAMARNVAFQEGRQICHFTTAVFDLQDLGTDWHREISKD
                     QAAYYFRPYKTILVRTVADGGVSYYVRGDHAVTFPNLYHSIIDHFQDGRE"
     gene            30223..31227
                     /locus_tag="PK898_02140"
     CDS             30223..31227
                     /locus_tag="PK898_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU18864.1"
                     /translation="MNSKRIAEILKNYPDLHILVAGDYFLDQYLMINRDLAEISLETG
                     LECHQVTEIRNSPGAAGTVANNLVALDVSVNALSAIGKDGRGFDLKTELLKRNIDTRA
                     LIESPLIMTPTYTKPMVQGKNEPLHELERIDIKNRSHLPIEIEDTLIENLENSFQLAD
                     GIVLVDQVQEENCGVLTDRVRNQIAALALKYPDKPVLADSRLRSAAFKNVTVKCNLSE
                     ALNVTGESDVERAAKKMVAGGARSAFITMGEKGIFAYSNGKGVTVPAVRVEGPIDICG
                     AGDSAAAGILSALCAGASFEEAAIIGNLTASITILQIGTTGTASREGIVRRAFEFFGE
                     "
     gene            complement(31235..32215)
                     /locus_tag="PK898_02145"
     CDS             complement(31235..32215)
                     /locus_tag="PK898_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU18865.1"
                     /translation="MTKPEYALALDFGGTKLATAVVSVPDGRLFGYQKARTPADAGAQ
                     ASLELILNTAGKSLKESGIPQEKITRGGISFGGPVNPDGKHVIRSMHIKGWENYPLAE
                     VAADRFGFPFRMGNDGNVAALGEWFYGSQGKPHYFMYIQISTGIGGGFILNGEILQGQ
                     GMAGEVGHQKVQYVGEAAFPCACGSKGCIEAVASGWALARDAKALIESPEACSVFREQ
                     VSKMGEISAKPLIEAARRGDSSASEIVDYAFRCFALSLSNAIVLFDLEEIVIGGGVAV
                     NSWDLLEPILTHSIPEFLPTEMRGKTKIRMSHLKGTETLLGAAKLSELAG"
     gene            complement(32212..32805)
                     /locus_tag="PK898_02150"
     CDS             complement(32212..32805)
                     /locus_tag="PK898_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOU18866.1"
                     /translation="MNSTKQEWVNEYIGLLNQAIDKMPVDQIVRAAEMIHSARLERKM
                     IFTMGNGGSSATASHMACDFSKNTRVPYLPAIRTMCLSDSTPTLTAFANDEGYEKVFS
                     EPLKALAEPGDILIAISGSGNSANILRGLETAREIGLKTIGLSGFKGGKMISMVDLPI
                     NIDTDSIERVEDLHMVCDHLLTMVLREIAMHELEGKR"
     gene            complement(32871..34520)
                     /locus_tag="PK898_02155"
     CDS             complement(32871..34520)
                     /locus_tag="PK898_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOU18867.1"
                     /translation="MPYPAVDLKKVRTVPLPARHNRVATDDFIFPSTPVAEFDSPDLK
                     EVAERILAARRAGRPLIWMQGAHVIKSGLAPILIDLLEKGYVQHVASNGAATIHDFEI
                     ALLGHTSEDVADSIKDGTFGMAEETGLLMNTAIQNGAKRGMGIGESLGELIGTDDRFA
                     QYRDVSVLYHTWRLGIPYTVHVAVGTDIIHQHPAVDFAAIGWGSGQDFKIFCKSVCDL
                     EGGVFLNVGSAVIGPEVFLKALSIARNLGNPLKVFTTANFDLIDLGDYRAPLGEDKVQ
                     YYYRPRKNVVNRPVMLGGKGFHICGNHKVTLPNLYQLLTANGETFPVESKRISLPMTE
                     MIDPSVRLILDEAIRLHPQIENVKEPIAQAYQLIRRSYSCGGTLFVAGNGGSMADALH
                     ISGELSKAFVVKRHMPAALAARLKKSEEGSVLAENLEEAQRVMVLGLNPVLRSAIEND
                     FQHPNLAVAQELYANAKPGDVMISISTSGNAENLHYAALLAKELGVYVICMTGSDGGK
                     MRKDADILIAVNQSQTDEIQNLHILIYHTLCKMLERHFGQE"
     gene            complement(34740..35690)
                     /locus_tag="PK898_02160"
     CDS             complement(34740..35690)
                     /locus_tag="PK898_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU18868.1"
                     /translation="MKKVTLIIVLAMLLSVCSVAMAAEKSYTIALITMDSMDQHWLKV
                     KAGAEAAVADLVKEGHDVKLVFDAPATKVDSVVQANMVENQITNKADAILLAPMDAEA
                     LVPAIEEVYDAGIPMILIDTLANTKKFHVIYQTDNGAAARLAADELGKLVDGKGEIAI
                     INAQAGSQTTMTRESEFRKEIAEKFPDIKIVGVQYCDGDKQKALNYAEDFMMKNPNLA
                     GFYACNEGSTVGTGNAVDQAGKAGVIKVVGFDFSDEVKDLVSRGVIQATMVQNPWVMG
                     NEGVKAAWKLINGEKVADEEAFVVDTGVTVVTKDNLDEVK"
     gene            complement(35748..36719)
                     /locus_tag="PK898_02165"
     CDS             complement(35748..36719)
                     /locus_tag="PK898_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18869.1"
                     /translation="MSQATVEVPTKQKAKMSRQKRQILSTLAALLMLFLFFTLANRNF
                     ISMMNILNILKQATPIIIIAIGQTFVLITAGIDLSIGSVIACSGVVAAIFMRDGMPVW
                     ISMVAGLLFGVLVGVINGSLIVYGKLPAFIATMGTMTTVRGLALFLTQGIPIGSLPKE
                     FTQIGTQRTFNVPNQVYIMAILAIIFGFVLSKTKIGRYTYAMGSNYEATRLSGVNVNL
                     TLIIVYTISGFLAAWAGMIMTANVISAAPTAGDGYELDAVASSVIGGASTLGGEGIIP
                     GTVLGALVIATLRNGLNLSGASPFIQKMIIGIVIIAAVFIDRFKRED"
     gene            complement(36732..38219)
                     /gene="gguA"
                     /locus_tag="PK898_02170"
     CDS             complement(36732..38219)
                     /gene="gguA"
                     /locus_tag="PK898_02170"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU18870.1"
                     /translation="MDDLLVQLVEISKTFPGVKALDHVSLDLRPGEVHALIGENGSGK
                     STLMKILSGVYMKDEGKVIVNGKEMNFQKPRDAQDAGIAIIHQELNLCRHLTVAENIF
                     LGREFQNGIFLDKKHQIEEAQKILDRLEVNLDPNSRVSELTVSKQQMVEIAKAISANA
                     NVVIMDEPTSALTIKEIDDLFSIIKVLRKQGKGIIYITHRLEELNAITDRITIIRDGQ
                     HIITTDFKNLSADDIIRYMVGRELKEKFPRIQMPVGKTIFEVKNLSAGKAVRDVSFTV
                     REGEILGIAGLMGAGRTETVRAIFGADKKDSGQLILNGQEIRIDKPQEAIETGIFCVP
                     EDRKRDGLCISMPVYQNATLPNMEKVTHRGIVNTKKEIMIAENMVKQLTIHTPSVFQI
                     VKNLSGGNQQKIVVGKWLVKPAHVILFDEPTRGIDVGAKVDIYNLMNQLKEQGIGVVF
                     VSSELPEILGMADRIVVMCEGRVTAELDADEANQEIIMQYATQYM"
     gene            39062..39535
                     /locus_tag="PK898_02175"
     CDS             39062..39535
                     /locus_tag="PK898_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18871.1"
                     /translation="MEKEDRFENGIPTSSLLNRLFKSSDLNDFLKENEKEMVIPTFME
                     HLDRLCRERNVIREHVINRSGIERCFGHQLFRGTRKPSRDNVLRLAFGFGLNVEETQE
                     LLRSAKKAPLYPRIKRDAAIIFGLSHQQTIMEIQSVLNDLELSLLGEEKYEHTER"
     gene            39516..41135
                     /locus_tag="PK898_02180"
     CDS             39516..41135
                     /locus_tag="PK898_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOU18872.1"
                     /translation="MNIPSADQILEFDDLKYPQDFLDQYELLECIAQKTESETFFVKN
                     RQSGEFAVAKCYIGKELISEPTEGQILNLLHHNGLPRFIEEYKNDETICVVREYIQGI
                     SLSERMKDGSLTERDAIPILLQLCDILTYLHHQCPPIIHRDIKPENIILAEDGSVKLI
                     DFGIARTYDKSEKKDTIIFGTQGYAPPEQYGFSQTDCRSDLFSLGIIIGYLLTGKTDR
                     EAVAKTLTNKRILHIYQKCTDFSPKRRYSSATNLKRALLRSDGRRQKTFFRFVKIALF
                     SVLFFGLGLIAGRFTDFPAFPAAEVTFDEPLIEKAVRLQINRADDLPITKDDLLSVTE
                     LYIFGNSVIAKTEDEMNRSAELLFQEGRMEPGSVVSLRDLNKLPNLRKVAVSMQRIKD
                     LTPLANLKQLQILILKNNPIEDLSPLNGLDSLERLSVFNTSVKDFSPLAECIRLRDLD
                     AGDTLVRTPIAFAPAKNLTHLNLYKLQMDTLAGVESLRNLEFIEISDVADDDLSPLLS
                     LKKLKTVKLSEKLRQAAETIANAAQFEIVYR"
     gene            41252..42526
                     /locus_tag="PK898_02185"
     CDS             41252..42526
                     /locus_tag="PK898_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOU18873.1"
                     /translation="MEKRTAKSLIIIFIFILCLGIKVPALAGSPSDAESQAEALKQLG
                     LMKDDPELSFSFDEELDRVQATVFLIRSTGKDAEAIYGKGSHPFSDVPAWADKYIAYA
                     YEKGLVNGESETTFGTEKADYPMVLTFLLRSLGYNDSEGDFSIKSPENLADAVGLLPD
                     DINEDECRYGDLVAVSWAALQADLKKGNQMLAEKLISENRFTASDYQKALESANTNAA
                     ETVSVSSFETLKSVLLNPDVRSVTVDSIGTPLIITGELTIPKGVTVTINRGNDFYIEN
                     NLINNGTIEVTGADSVSPNFINYSVMAIQKGGKVTNNGKIVLQPSVLADTEDRGPVGG
                     QLRIFDGTFENNGSVYLKAGKVNTHGGMVAVIEEPFTNNALIIVDGFQIDIAGTFTNN
                     ADGIIINNTYISTAKGGTFINNGSLLGEKIVE"
     gene            42624..44231
                     /locus_tag="PK898_02190"
     CDS             42624..44231
                     /locus_tag="PK898_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18874.1"
                     /translation="MAPIKGSKEYVKRNKEPVKTNENLKSVLEFLEISNLALAKALDL
                     DPSLISRYLSGERRLFAASAQMNAIADFIIKRCDQLRDIQWLTDQFHSAGLLTDVSAT
                     GQFKQNLILWLASDGEILRKNFGLAKPADIGHFAPREKNRSISTMDEVQIGGLSIVSE
                     LFQILQNLPQKSVIDIFISNDRIGTITDQDISNTLLKQIEKNDLHVRMIVCVSGDTQA
                     MSQILNTYISALVSGHIRLSVIFAVTQTVINQMTLLIPDRYVLLITETNGGTAPPVAV
                     PVRNSQFITDMGRNFEMAWHYGQPLLKLYDDNFSRNILEILFTEFCSPGPLDVVKDSI
                     NPMYMPVKAYDRFLKTRGHHQDEFSWRSAEFARFKKGMDNVLAADVPFREILSMSRLH
                     DIVSKGSCRMAGLYFMERGYIDLDTEGCIAILNGYIEYLKNVPNFNLVIVDDLNQLHR
                     NNCWQLKQNHHLAINYWNGPEPIMIYSNQIMLLREFQTHFEMLWEQGQKSLPNRANVI
                     AILQNIIDHLIENFGTEQKDNQDSYPI"
     gene            44283..45254
                     /locus_tag="PK898_02195"
     CDS             44283..45254
                     /locus_tag="PK898_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18875.1"
                     /translation="MKKHMMVIGLISLFYIFITCGATMHRSFKTQDVSVSAGENFKFG
                     DETYALEKWIKMPKYDSESKTGYGLSFLQKEGVMPVQLSISTNGSPSAYASLLDMKLE
                     IDGENLEPENISFENVKDVPGYTARIIFEFAVPNQSKLPDKGTLFYTNKKIQKEIDLS
                     ALEAPEKNIEISAENPEILNFCGNWKLSSIIFHTPDTGNGIFEIDATLDISDGFEYGR
                     DLTLSKDMSLKSDVDIDALIDAISELPFIVNDFNLKGDTGWSYSDGKMKTIPDGTELM
                     ASYNEKDEVLLLVHSGKVDILSKKTDKNAVKSGPVLMEITAVFKKAN"
     gene            45269..45562
                     /locus_tag="PK898_02200"
     CDS             45269..45562
                     /locus_tag="PK898_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18876.1"
                     /translation="MERKVLCPVCGLSNDFVSPRRMRCRSCGNNFEITENDFQNVKFI
                     EINDGYHSWQSQPTAYHTIYACLSYPEYCDLCIHKNDLFCRNGKIDFREKRIK"
     gene            45720..45983
                     /locus_tag="PK898_02205"
     CDS             45720..45983
                     /locus_tag="PK898_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18877.1"
                     /translation="MRNPIRKWQEKRLAKKQQKEKEAAEKKILFEQRLERCKKGEHEW
                     QYSYEVQGDGDPNVEGYVLITKATCQVCHETEVEIEPYDKEDD"
     gene            complement(46063..47019)
                     /locus_tag="PK898_02210"
     CDS             complement(46063..47019)
                     /locus_tag="PK898_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOU18878.1"
                     /translation="MKLVVLDGYTLNPGDIGWDKLEKFGDLTVYDRTNTGEVSDRIRD
                     ADIVFTNKTYVSRESIYAAPNLKYIGVLATGYNSVDIQAAKERNIPVTNVPSYGTEAV
                     AQFAIGLLLEVCHQIGHHNRVVHEGKWEACPDYCFWDYPMIELSGKTMGIIGLGRIGT
                     CTGRIAKAMGMNVITSTKELSPEGEKIARQVSLDELLKDSDVIMLHCPLTPETKEMIN
                     KETIGKMKDGVILVNNSRGQLIAEQDLADALNSGKIYFAAVDVVSEEPIKHQNPLLKA
                     KNCIITPHISWTSKESRMRLMSIASENLEQFLAGHPINVVNG"
     gene            complement(47071..47838)
                     /locus_tag="PK898_02215"
     CDS             complement(47071..47838)
                     /locus_tag="PK898_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOU18879.1"
                     /translation="MFVEERQREILEFLHQNGKVLVKDLSERYQVTPDLIRKDLAKLE
                     KEGLLKRTYGGAIPLIQNSVPQPADDSHENNIEVKQRIAEKAETLIRNGDTVFLDLSS
                     INVEIARQLVRKNRNVTIVTNMINVLEEIPGESRVKLIFIGGKMNKLREGFTGAATIE
                     WMSRFQFDLSFIGVVGIDLVRNSATTYEIDDALTKTGAIKISNKSYIVAESSKFNSTG
                     NCRSASLNSFSGIITDAGLDPEIVEKLTQIGLQVLLV"
     gene            48004..48288
                     /locus_tag="PK898_02220"
     CDS             48004..48288
                     /locus_tag="PK898_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18880.1"
                     /translation="MIRYSRPLEDGSNYFIPEDRIVRIPAGYTGEPINRLAAYENLQQ
                     DLLNDLEKISQEMAGLREEGKIKSVHFKELMSQKMINTRIISLFQSYGLE"
     gene            48402..49100
                     /locus_tag="PK898_02225"
     CDS             48402..49100
                     /locus_tag="PK898_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOU18881.1"
                     /translation="MVNHTFTDENSIQRNHYPVKIGNLFKLSFGEEVGNSVSHGVSAM
                     ILLFALPACAIYGYQKGGKLLAFGYSIYIISLLLMFLASCLYHAMDFNSKHKQVFRIL
                     DHSAIYIAIAGTFTPVCLSVVGGWLGIVVLICQWVTAIFGILFKSLSNKNLPKASLTI
                     YLVMGWSAILLLPAIIRKQLWGFLAFILLGGLLYTVGTIFYSKKEKGWFHFIWHLFVI
                     GASAAHFVAIIFYI"
     gene            49146..49571
                     /locus_tag="PK898_02230"
     CDS             49146..49571
                     /locus_tag="PK898_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU18882.1"
                     /translation="METFWPIILIVISNIFYNITTKSTPADANPFLSLTVTYLVGAIL
                     TFLMFWATATEKGLSSNISRLNWTGYCLGIIIVGLETGYIYLYRAGWNISLGSLVANI
                     LLAISLVIIGALFYHEEISARQLIGMLFCIIGLIFVNKK"
     gene            complement(49681..51219)
                     /gene="msrAB"
                     /locus_tag="PK898_02235"
     CDS             complement(49681..51219)
                     /gene="msrAB"
                     /locus_tag="PK898_02235"
                     /inference="COORDINATES: protein motif:HMM:NF010625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HOU18883.1"
                     /translation="MLILILIISGCSGSSKSGENPNEIDLVDLDGQRFKLSEQLGKKV
                     YIKFWASWCPICLEGLPELEKLASESNDFEVVTIVSPGLGKEMDTNEFREWFAGLENN
                     HFRVLLDEGGKIATKYGVHGYPTSFFVSANGTIKVFPGEIKNEMVKAIFKSNLLDGES
                     DPVQENMQKEQEEMEPNSENDAIRGSANSQPSAEAEIYLAGGCFWGVEAYFEKIDGVK
                     EVTVGYANGKTENPSYEDLVYNHSGHAETVHVIYDPSRVTLDQLLQYYFRIIDPTSIN
                     RQGNDRGVQYRTGIYYVDESDKAVIETRIAMEQKKYDKKIQVEVTPLIRFDLAEDYHQ
                     DYLKKNPNGYCHIDLSLADEPLIDETAYPKPSDSAIKGMLTEEQYNVTQYSGTEKPFT
                     NAYWNTFDDGIYVDIVTGEPLFSSLDKFDSDCGWPSFSKPISDEVVNYHTDTSYGMVR
                     TEVRSRSGDSHLGHVFDDGPAESGGQRYCINSAAVRFIPVSKMEAEGYGYLLKLFDPS
                     KKVD"
     gene            complement(51266..51946)
                     /locus_tag="PK898_02240"
     CDS             complement(51266..51946)
                     /locus_tag="PK898_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOU18884.1"
                     /translation="MNQNLMIGSVFTAGILSFFSPCILPLLPVYFGILTNTSTPENSI
                     EAKKDTGRNLLKTILFILGIGFVFILLGFGAGSLAPLFSNPLFHRIGGLIIILFGLNQ
                     MGVLRLNFMEKWNAPGYNSSGKNQYLSAFFLGLIVSLGWTPCIGPILASVLILAANSG
                     TAIRGAWYMTFYTLGMAIPFVVIMLFADFLLIRIKKGGQWLPVVRKIGGALLVITGLL
                     LFFNRLNG"
BASE COUNT        15029 a        11417 c        10829 g        14766 t
ORIGIN      
        1 tcatacctca atttccccat tcatcagttt gggtaataga gaatcccgaa ttttagcgag
       61 aattaccgat tcttctaatc cattaataat gcagtggtaa tagtttccaa caatttcatc
      121 aaaaagagca agaaggtcat cattgggaat tatgatatta atatccaaaa tatcttgggg
      181 aatgattctc tgatggctgc tacttgtccc tgaagctctt gacttcatag tgtcgatcat
      241 tgatgtttga gagaagaggt tagcagcgaa tgaatagtaa tgcgcttttt gaggcaatag
      301 aacctgaaat tcagttgaac aaacactgct ctcttgattc acttctttca atggccaaac
      361 tctgggtatt cgtgggttta atttcgacac taaaatagaa ttgctcttta ctttatattt
      421 attgcttaat attgcagagc ctaattcttg tgatggaacg cgtccactgt caaatgctgg
      481 aatactgtaa tggtaaaaca atttatcagg attactccct ggtgatactg tttcattggc
      541 aagaattaat atctccgcaa cagaaccaat tctccatccc ttgggaatca tgcccagctc
      601 gctctcgacc aattcaccgg tggcgccggg aaagttgaaa tcaacaaacc actccttgaa
      661 aatagcctgg gcgatggctt ccagggtggc attggtctga cgattgagct cgattttgtc
      721 atccagtgcg gagagaatat cagcaattcg gcgttgagtg gaaataggag gaaggttaat
      781 ctcaatatga tcaaatgttt cccgagttat cgtatcgaat accgcaccat gagtctttct
      841 ttttatttgc tcaatattat atttaagcaa atagtataaa aaatcattag aggtcatcgc
      901 tttagaattt aatccataac aggattggtt aaaagccata tcttttccta aaatagcgag
      961 ttctccgaca gttcccctag ctgatatgat tatttgtcct ttttttagaa tttttgcgct
     1021 tgagttatct aaccccaatt gtgttatgct tttttcagtt tcaaaaacat attttttttc
     1081 acccccaaaa tccacaaccg atagccatgg aatgtttcca ttccaatact caacaatatt
     1141 agttctagga gtccctccgc ctatgatttc cataatttcg gaaagcttga atttttccca
     1201 ctcattagaa ctcataacca atcccctcta aattcttgca atttttggtt tatacatcaa
     1261 tcctttaagc gaacccctta gaaacttgag caatttgtga acttattatc attttcatgt
     1321 gttttccttt tttgcatgag cgaacctatc atgttcaaat aatttgaaaa atcgaacaag
     1381 ataaatctat catgttcaaa atttgcctaa ttttgaacat gatcagcttt cataattccc
     1441 ttgcaacaat ttgaataaag gcacattaat gtaataattg ctgcgccaga ttttttgttt
     1501 ttcaagaaag ccggcttctg ccagagcatc taagtaagaa cctgccgtaa cccgcttcac
     1561 gcccacatcc cggatgacaa aatcaatttt ggtgtaagga tgtttaaaaa gggtgtttaa
     1621 caaatccaac gaataaattt ttggcagttg cattttgatc ttttcttgag tatcagccat
     1681 caggtctctt aactgatgaa tggtctcgat agtttccata gaggttaact ccacaccgcg
     1741 gagcataaaa gccaaccagg cttcccaatt gtcatccaag ggatcggttc gtaattgggt
     1801 taggtaagca tttttttcgc gaataatata acggctgagg taaagcaccg gaagatccaa
     1861 cagaccgtgc tgtaccagat aaagcagatt taaaatacgc ccggtccgcc cattaccatc
     1921 ataaaatggg tggattttct cgaactggta atggatcaaa gccatccgta ccaacgggtc
     1981 gagtccttcg tcaacagcct cattgaaaaa gttcagaagc cgctcaagca gctccacaat
     2041 ttggtcatgg tcttggggcg gagtgtaaat cacttttcgg gtagcggagt tcccaacaat
     2101 tgttcccggc cggctacgga taccgggtcc cggttctccg attaccaaaa cagcttggat
     2161 cgcgaccaaa tcttccaaag aaagttcccc tttggtctca atacgggaaa aaccgagctt
     2221 aagcgcctca gaatagcgtc caacctcttt tgcggcggca ttgttgaatt ggggaatatc
     2281 caattcttgc ttgaacagtt catcatgcga ggtgatgatg ttctctatag ccgaactgtc
     2341 tttcgcctct tgcaatgcca aggtattcag caggatatct tgattgggca gcaacggtgc
     2401 aacgcctttg agttcagcca gccggcggtg tgccatgaca atttgcctat tgagtgtatc
     2461 ggattgagcg actttttcgg gcggaaacgc gtataggtca gaaatcatac ccgatcccct
     2521 tcaaattttc gcgaatccgc tgctgaagct cattcccgcg cgcaaactgt tcggccaatt
     2581 gtgcggtcag cgtattcatt ttttcttcaa aaggaatccc atcatcttcg gcttcttctg
     2641 tgcctacata ccgcccgggc attaagacgt aattattggc gcgaacttca tccagcgtag
     2701 ctgctttgca aaaccccggt atatcttcat aaacaccgct ttctccacgc caagcatggt
     2761 aggtgccggc gattttggca atatcttcat cggtgaattc cttattgcgg cgattgatca
     2821 tcgttcccag tttgcgagca tcaataaaca atatttcatt cgaacggttg cggaatttat
     2881 ggttcgttcg atctcgagcc aggaaccata aacaggccgg gatcatcgta ttatagaaaa
     2941 gttgagaggg aagagcgacc atacaatcca ctaaaccgtc ttcaatcata ttttttcgaa
     3001 tttcaccttc tccgccggta ttgctattca tactgccgtt cgccagcaca attccggcga
     3061 tacccgttgg gctaagctta tgggcaaaat gttgcagcca ggcataattg gcgtttccga
     3121 ccggcggcac cccatatttc cagcgaatgt catcgcgcaa ctgttcacca ctccaatcac
     3181 tcacattaaa tggcggattg gcaaggataa agtcggcctt cagatccgga tggcgatcat
     3241 taaggaaagt gtcgcccaat tccaatttgg cgtcaatacc gcgaatagcc aggttcatcc
     3301 gcgccagacg caaggtggtc ggattggatt cctgcccgaa gatcgataga tctttgatgc
     3361 tgccctgatg attgcggaca aatttttcac tctgaacaaa catgccgccg ctgccgcagc
     3421 aaccgtcata tactcgcccg ttgaaaggct caatcatttc caccaacagt ttaacaatac
     3481 tggcgggagt ataaaattgg ccgcctttct tcccttcggc gtcggcaaat tgacctaaga
     3541 aatattcata aacgcgtccc agcagatcct gagaggtgtg cgcatcctgg tgaaaaccga
     3601 tggttccaat gagatcaatt agttctccca gacgttgttt gtttaactcg ggatccgcat
     3661 aaatcttagg cagcacaccc ttaagcggtg ggttaattcg ctcgatggca tccatcgcat
     3721 catcgagata tttcccaatt tccggttgct ttgcccgatc ctgaagatat tgccagcgcg
     3781 ctttttcagg gacgaaaaag acattattgg ctaagtactc atccggatct tccggatctg
     3841 aaccttcata ttcgccttgg ttttctgtga gcttttggta cagatcctca aaagcatcgg
     3901 aaatgtactt cagaaaaata agtcccagta cgacatgttt atattccgcc gcgtccatat
     3961 ttgagcgcag tttatctgcg gctgcccaaa gcgttttttc cagttcatgg ttgttttctg
     4021 tcatctgctt ctcctgtcta aatcgtcaca gcggttgcgg tctgtcattg attttcaaat
     4081 ttcccgggtc tgcgttttgc tttttctgtt tcctaaaccc cttatgaaat ataactcttc
     4141 gaaatcgcta ttgtttgcgg attttaaatt cagaaatgat cttatcctgc gtaagtttgt
     4201 aacctgcggg tcaatcccat caccggtatt tacgaattca ttgataccgg atactctaat
     4261 tattatagca ttggtcaaga aaacgatctc ctcccgggtt tccaagactg ccgccggatt
     4321 cattccaaaa tcccccagaa tcgattaaga taaatttatg gatgattttt cttataattc
     4381 gcaatacttg atccggaatg gaaagccttg gttcccgctg atgggagaga tgcactattc
     4441 ccgcgtagac cgccgtttct gggctgattc gctgcaaaaa atgaaagccg gaggaatcga
     4501 cattgtttct tcttatgtca tctggattca tcatgaagaa atcgagggcg aattcgactt
     4561 ttccggaaat aaagatttac ggaaatttat tcaattgtgc caagatcacg gactttattt
     4621 tttcctgagg atcggaccct gggttcatgg tgaagttcga catggcggct ttccggattg
     4681 gctgctggcg aaaggtttcg aagttcgcac aaatgacgag cgctattttg cggaagttta
     4741 tcggttttat gaccggatct attcgcaaat caaaggattg caattgaaag agggcggacc
     4801 gatcatcggc atccaaattg aaaacgagta cgggcactgc ggcggtctta caggaccgga
     4861 gggcgaagcc catatgcgac gtttaaccgc tatggcaaag gaaatcggtt ttgatgtccc
     4921 ctatttcacc gccaccggtt ggggcggtgc cgttaccgca gggttattac cggttatggg
     4981 aggatatccc gacgcgcctt gggacagcag attgaccgaa atcgaaccca gcgggaatta
     5041 catatttacg ccggaacgga acgatcacaa catcggatcg gatcatcata tcggttatgg
     5101 attaacattt gatccggctg aatttcctta tctaaccgcc gaattgggag gcggactgca
     5161 agttacttat catcggcggc cggtcgtaac ggcggccgat atcggtgcca tgacattggt
     5221 taaactcgga tcgggtgtta atttgctcgg ttattacatg tttcgcggcg gatcaaaccc
     5281 gcccggtaaa cgatcttcct tgcaggaatc gcgagcggcg ggtgactttt gtgatctgcc
     5341 gcagatatca tatgactttc gcgctccgat tcgagaattc ggacagattt cggattcgtt
     5401 tcgtgagatc aagcggtttg ctctctttct tcatgacttt ggtgaggagc tttgtaaaat
     5461 gccggctaaa tttccgagtt caaatccgct tgatccgaaa aacaatcggg atttgcgcac
     5521 agccgttcgt gataacggaa aaaatggcta tttgtttgtc aataattacc aacggcgaca
     5581 aaagatggcg gatcatccgc aaacaagcct gcgggtgacg ttacccgatg agacaattga
     5641 gttcccgata acggatattc gtgacggaga ttatttcttt ttcccgttca accttcctat
     5701 cgggaattca atccttaaat cagccgccgc cacccctctt tgtaaaatca acgatgacac
     5761 gaccgtcttc tattccgatt ttccgccgca atatcaattc gatcatgaac ctttgaatca
     5821 caaaatcata accctcagca aaaagcaagc gatgaatgcc tggaaaattc ggcttccgga
     5881 cggggatcat ttgctccttt cggaagatgt ggttttgcaa aatcgggatc gattggagat
     5941 gatcggtcgg ggaatcgccg aattgagcat atatcccgat ttcccgaagc cgccccacaa
     6001 tgccgtacga atcggtgggg aaggtgattt taccgtttat gaagtgcggc atccgggatt
     6061 tgatccgaat cgaaatccgg ccgaagcaaa gttccgaatc gtcaggcaga cgccggaaca
     6121 gacagattat gagatcaccc tcaactatcc caccgatgtt gaaaactgtt acctttccgt
     6181 tggttatcag ggcgatcagg caagattaat cgtcgacggg aaactctcag cggatgattt
     6241 ctatgcggga acggaatggg aaatcgggct gaaacagttc aattttccga aaaaaatcat
     6301 tttgtccgta acgcctttgt atgccggcat gcctgtttat ctcgagcaat ggccgataat
     6361 cgaaggtgaa aaaatttgcc ggctgaatca tatcagcctg aattgtgaat atcggacaat
     6421 catcaatcgg atccggtaaa tcttcatatt tttcctgtga tttatttcct gcgatttttc
     6481 ttaccgaaaa gataaaatca ggaaaatcat cccgatttaa gtaaaaatga tcaaatcatt
     6541 cgctgttcgt tacgatcatt ttgggtgaaa gatgactttt ccgaaagtcc ttattgacaa
     6601 tccttttttt tcgatgtaaa tttaacttac cggttagtta aatcagatat aaagagctga
     6661 aaatggaaaa aaatcatttc tcccgggaag aagcgaaaga gcggattatc ggcgcttcag
     6721 ttaaactatt ctccgaaaag ggttttgacg ggacccgtgt aaatgaaatc gccgaagccg
     6781 cggacgtgaa caaagcctta atttattact atttcgataa caaagaagcc atcttagatc
     6841 aacttttgaa ccaaatcttg aataatgtca cttcactttc gacaacattt gttcaaaaga
     6901 gtatccgcca aatgatacaa gacggacggc tctgcatcat gaaagatcgg ctccattttg
     6961 ccaacgaagc cgctcttgaa aatttcctgt cccatattca aaatttcaat aatcaaattt
     7021 tggattatgc catgaacaat cgaaaactga tccgaatttt gatgaccgaa tccttgaagg
     7081 aaagcaagca taaaaatgat ttgattcaat ttatcaaaga gcttggaaag gcagccgaga
     7141 accccattat tcgaaccatt cgcgaagcca atgccgattt tcctctgtcg aataacttta
     7201 tttttctgag attcttcttt catcctgttt cgatcatcaa ttttgcagtt tactatgaga
     7261 cgtatcaaaa aatttgcggc ttatccgaat ccaaaatgcg aacggctatg ctcaattcga
     7321 tccggacact ttcaaattca ttcgtttcgg gaaacgatat cattttacaa acggaggcat
     7381 aaaacagaat ttcattaatt tatttttaac gtcaattcaa tgaccgacga ccgatgattt
     7441 gtatagaaat gggaatagta aaggaggaaa aatgaaaaat atacaagatt caatcgaaga
     7501 gctgattatt cattatgtcg tgacggatcc gttgggaaaa ctgcctaaga tgctcaactt
     7561 ggcggaaaaa atagatcagg gtcacctgca tgaaaaagaa attaatggta tccgcaaaat
     7621 cctggatgat aaaaacggta actggtacaa atttattgaa ggcgtcagcg aggatatcga
     7681 tccgaaatgc ctgcaaaaag tgattgaatg cttcatgatc aatgccagtt tgaaaggtga
     7741 agcaaaggcg cgagcggtcg agaaaaaata tgatatgaac gttccgtgga cgattctggt
     7801 tgatccgacc agcgcctgca atctgcattg taccggttgc tgggctgccg aatatgggaa
     7861 taaaaacaat ttatcttatg agacgctcga ttcgctcgtg aatcagggtg tcgacctggg
     7921 aatctatttt tatatctttt ccggcggcga accgcttatc cgaaaagatg atattatccg
     7981 gctttgcaaa gctcaccagg attgctattt cttttctttt accaacggaa ccctcgtgga
     8041 tgatcaattc tgcaaacaga tgcgggaagt cggcaatttc gctgtcgcct tttcgatcga
     8101 aggaaatgaa gaggcaacgg atatgcggcg cggtaaagga acttatcgaa aagttatcgc
     8161 cgcaatggac ttgatgaaaa agaataaact gctgtttggc tattcaacct gctatcatcg
     8221 ctataatacg gagagcgtcg gctcggatga atttgtcgat gatatgattg cccgcggctg
     8281 caaattcgca tggaacttca cttatattcc ggtcggaaag gatgccgtcc ccgatctgct
     8341 ggcgactccg gaacaacgga aatatatgta tcatcgcgtt cgtgaaattc gggcgaccaa
     8401 accacttttc gcaatggatt tttggaatga tggagattct acaaacggct gcatcgccgg
     8461 cggaagacgg tatctgcata ttaatgccgc cggagacgtt gaaccttgcg catttattca
     8521 ttattccaat gccaatattc atgatatgtc tctgttggat gctttgaaat caccgatctt
     8581 cgcggaatat cgtaaaggac agccgtttaa tggaaaccat ttacggcctt gcccgctgtt
     8641 ggataatccg caggctttgg caagaatggt gaaagcttcc ggtgcaaaat cgacggatat
     8701 ggaagctccg gaagacgttg acgtattgac ggcaaaaacg aaagcggcgg ctgagaaatg
     8761 ggcaccggtt gcggatgaac tttgggaaaa acaaaataaa gctaaggcag aaaaacaagc
     8821 cgaagcgcaa aaagtggaaa tagaagcctg agccaatcaa atgaaacgga aagcgatgaa
     8881 ataaggcttt ccgaagcgat taaaaagagg agcgataacc gattgaaggg tttccgtcat
     8941 gagaattgct gactgtcgac aattctcatg atgttcctct tgttttagaa ggctgctgaa
     9001 aatattgacc gatcaatttc gtcggttttt acgcatgatc tcggcttgac aggattgaaa
     9061 tccggcggta aatttaacca aacagttaaa ttaaaaggcg gaagaatggt tactcgggta
     9121 attgaatcgg aagactcaaa agatcgaatt ataaaggcgg ccgttcagct tttttccgaa
     9181 aaaggatttg acggaacccg agtcagtgat attgcggatt tggcggatgt caacaaggcg
     9241 ctgatttatt attactttac tggaaaagaa gatattctgg atatcttgct ccggcagttg
     9301 ttgaacgata tcaccgcttt ttcaatggat tttatccaca cccaccttat ttcaatgatc
     9361 cgtgatggaa ccttggaact aaaagaagat cggctgacat tcattgataa aaatgcggtg
     9421 gactcttttc tggacagcat gcgcggttat tatcggaagg cggtttctta tgcattggaa
     9481 aatcggcaaa tcattcgtat tctgatgctg gaatcactga aaaattccaa acaccagaat
     9541 gatttatatc ttttcctgaa attgctcaat aaaaatgaca acaatcccgt ttataagaca
     9601 atcctaaatg ccgactggaa ctttaattat tccgatgaat ttattctgta taaattcttt
     9661 ttcagtatga tgcctatcat caattttgcg gtttattttg atgattacaa aaatcattcc
     9721 gacatggaag aagaggattt gattaaccgt tttctgcaat cctgccaaac aaccatcaat
     9781 gcattggttt gcgggaacga aatattgatg cgaaatgatt gctcgattat cgaaagcaga
     9841 taatttttgt agtagcgaat aaatagaaag gggtaaatta aaccaagcat gccgttttag
     9901 aaatgttgac acgtttcaat cggttgccgg taagctaagg agaaaatgaa tgaatagtaa
     9961 agttcaaaat gcaatgctct cactgttgat gggatacctg gaaaatgatc cgatgaataa
    10021 aattcccgca attctggatt tggcggaaaa aatggatacg ggtcatgtgc atgccaattc
    10081 gtttaaagtt gttcgcagag aattaaccga caaaaacggt gtttggtatc aatttacaga
    10141 aaacctgttt aatgaagtcg acccgcgagt gattaaaaaa gcggttacca gttttttctt
    10201 caatgggatt ctcaataacg gaactaaaca agacaaatta cgtgaaaaat atcaatgtaa
    10261 tattccctgg gcaattttaa tggatcctac cagtgcttgt aatttgcatt gtacgggctg
    10321 ttgggcagct gaatacggaa atcggaataa tctttccttt gaaactctgg attcaatctg
    10381 taaacaggga aaagagttgg gaatttattt ctatctgctt tcaggcggcg aaccgaccgt
    10441 tcgtaaagag gacgtgatta aactatgcga agcgaacccc gattgctttt tcttcgcatt
    10501 tacaaacgga acactggtgg atgataaatt ctgcgatgaa atgttgaggg tcgggaatat
    10561 cactttgggt ttctccatcg aaggagatga agcggcaacg gatatgcgac gagggaaagg
    10621 gacctatcaa aaagtgatcg cggcaatgga tcgcatgaaa aaacataagc tgcttttcgg
    10681 ttattcgacc tgctatcatc gttacaatac cgaaagcgtc ggttccgatg aatttgtgga
    10741 tgatatgatc gcccacggtt gtcgctttgc ctggaatttt acctatatac cggtcgggaa
    10801 ggatgccgtt accgatttgc tggcgacgcc cgaacagcga gaatatatgt atcgccgcat
    10861 tcgcgaaatt cgcgcgacca aaccgatttt tgcgttggat ttctggaatg acggggaata
    10921 cgtcggaggc tgtattgccg gcgggcggcg ctatctgcat attaatgccg ccggtgatgt
    10981 ggagccttgt gctttcattc attattcaaa tgtcaacatt catgatgtaa ctctgttgga
    11041 agctttgcaa tcaccgttat tcatggctta tcggcaagga caacctttca acgaaaacct
    11101 gattcgcccc tgcccgcttt tggataatcc gcaggcactt tccaaaatgg tcaaagtttc
    11161 caatgcaaaa tcgaccgaca tggaagcgcc ggaagatgtc gatgttttaa catcgaaaac
    11221 gaagccggca gccgagaaat gggcgccggt cgccgatgaa ttatgggaaa aacatttgaa
    11281 ggaaaaagaa gagaaagaaa aggaaaaaat tgcgggctga ttcgaacccg ccaacgttct
    11341 catttttatt cttcctatcg aaaaatgctg ccgaattata ttttggcagc atttttctta
    11401 tggaaacatt cgataaaata agatctgacg acaaaatatt tattgtcaca cagaatcaat
    11461 gaatttcagg agggagctcg atggatgaaa tcaaaacaat cgttgatacg caacgtcgat
    11521 tctttttaag cggaaaaaca cttccggtct ctcagcggaa aaaggcgctg caatctttgc
    11581 tgacaatggt ggatgaaaat cagcaaatga tttttgatgc cttgtttacg gatttacata
    11641 aatccgaatt tgaaagttat atgaccgaag tcggacttgt tttaacggaa attcgtcagg
    11701 cgatgaaaaa cctggacgct tggacatccg ttaaaaaagt ccggactcca ctgtatctgt
    11761 tcccttcaaa aagcagcgtt tatccggagc cctacggaac cgtcctcatt atggctcctt
    11821 ggaattatcc gttccagtta tgtcttgtcc cattaatcgg cgctttggca gccgggaata
    11881 ccgccgtcct gaaaccttcc gcctacgcac cggcagtcag tcgctgcatc gcagagttga
    11941 ttcaccggta ctttccttct gaatatatag ccgttacgga agggggacgg gaagaaaaca
    12001 gctcgttatt ggaacaacat tttgacttga tctttttcac aggtgggagt cgcatgggaa
    12061 aaatcgtcat ggagtcggct gcgaagaatc tgacgcctgt tattcttgaa ttgggaggaa
    12121 agagtccgtg catcgtcgat caaaccgctg atttgaaaaa tgcgtcacgg aaaattgttt
    12181 tcgggaaatt actgaatgcc ggacaaactt gcgtggcgcc tgattattta ttggttcatg
    12241 agtcggtaaa agatacgctc atccacaatc ttatcgagga gttcgacaaa gctttccccg
    12301 acaaagacta ttccgatttt ccggcgatca tcaacgaaaa acattttgat cgactgaacc
    12361 ggcttatccg ggatgaaaat gtgatttatg gcggaaattc gaatcgggat cgtcgctttt
    12421 tcgagccgac gattttattt ccggttcaat gggaaacacc ggtcatgcaa gaagagattt
    12481 tcggtcccat attacccgtt ttgacttatg agaatttaga cgatgtcatc gatcggttgc
    12541 gaaaacttcc caaaccgctt gcgctttatt tatttaccga agacagaaaa accgaaaaga
    12601 aaataatcga aaatgtagcc ttcggcggcg gttgcatgaa tgatacggtt atgcatttgt
    12661 ccaacccgaa tctcggtttc ggcggaatcg gtgagagcgg catggggcag taccatggaa
    12721 agaacagttt tgattccttc acccattata aaagtatggt taaaactccc gtttggcccg
    12781 atttgccgat ccgttatcat ccatatacaa agcaaaatct aaaccggttg aaacggtttc
    12841 tcaaataaat gcggtaaatc cgccattcta tttcctgttc gaaagaaatc atccggattt
    12901 attaatcttg ccggttgacg aaagctatat aatgaattaa gaagatcttt ttcaggaggt
    12961 aaaaattatg tcaattattg ctgcttatat tgtgccccat cctccgatta ttttgcctga
    13021 aatcggaaaa ggggaggaac gaaaagttca aaaaacaatc gacagttaca aaaaaattgc
    13081 cgctgaaata gccgaatcgg aaccggaaac gattgtgatt acttcacctc atgccacact
    13141 ttatgcggat tattttcata tctcacccgg gcgtcatgcc gccggtgatt tcggacaatt
    13201 cggtgcgcgg ggactggtga tctccgcaga ttacgacgaa gagttcgtac aggaactgga
    13261 aaaaaccgcc gaagaaaata acattccggc cggtaccgcc ggtgaacgcg atccccgatt
    13321 ggatcacggc acaatgatcc cgcttcgttt cattcagaaa gcgtgggacc ccaataaaaa
    13381 gccgtttaaa attgtccgaa tcgggctctc cggagaatct ttattaaccc actatgaatt
    13441 tggcaaatgc atcgccgaag ttgccgaaaa attgaaccgc agaacggtat ttgtcgccag
    13501 cggcgatctt tcgcatcgat taaaagaaga cgggccttat ggattcgtca aggaaggtcc
    13561 gattttcgat caacagatta cgaatatcat gcgcagcggt aatttttccg aaatattcaa
    13621 tttggacccg catctttgcg atcaagccgg ggaatgcggt ttacggtcat tcgtgattat
    13681 ggcgggagca ttggacggga aacagatcga accggaatta ctctcttatg aaggtacttt
    13741 cggagtcggt tatgcggttg cttcgtttaa aatcaaagga atcgatgaaa acagccgttt
    13801 taatcgatat gagacggaat ttcaaatcaa caatggagaa gatccatacg tcgcccttgc
    13861 aaaagcatct ctgaccactt atctgaagac gggaaaatac atgaaagttc cttccggatt
    13921 gccggccgaa atgttaaatc gccgagccgg aacttttgtt tctctgaaaa aaaatgggga
    13981 gctgcgaggc tgtatcggta cgattctgcc ggtacgatct tccgttgccg aagagatcat
    14041 tcataatgcg gtatctgcag gggtcgagga tccccgattt ccgccggtta ccctcaacga
    14101 actggatgat cttgatttca gcgtagacgt cctttccgaa cccgaagaca tttcttcacc
    14161 ggatcaactg gatgtaaaac gttacggtgt gattgtttcg agcggttatc ggcgcggatt
    14221 gctgttaccg gatctggagg gggtagacac cgtcgaggag caaatcgata tcgccagacg
    14281 aaaagccggc attccgagag gagaaccgat tcgtttgcag cgatttgaag tagtgaggca
    14341 tacatgaaag caacctgtga aatttgtttt catcattgtt cgcttgccga gggccagatc
    14401 ggtttttgcg gcgcgcggaa aaacgtcgac ggaaaagtta tctgtgataa ctacgggaaa
    14461 attaccgcca ttgcccttga tccgattgag aaaaagcccc tgaaacactt tttccccggc
    14521 agctatattc tgtctatcgg cagctatggt tgtaatttac attgcccctt ctgtcaaaac
    14581 agcgatattt ccatgcaata cgcaggaatc cgcactcatt tcatttctcc gcaggaattg
    14641 gtgagcaaag cgctggagtt gaaatctgac gggaacatcg gagtggctta tacctataac
    14701 gagcctttta tcagttatga atacgtgacg gattgcgccg ggttagtaca tcaaaacggg
    14761 atgaaaaacg tgatggttac gaacggaacc gtcgaagagg ctcctttaag aaaaattctg
    14821 cctctgatcg atgccatgaa tatcgatctc aagggattta ccggtgaata ttatcgaaaa
    14881 ttaagcggca atttggaagt ggtcaagaga acaatcgaaa ttgcagccaa agaatgccat
    14941 atcgaagtca ccacattgat cgttcccggg gaaaatgaca gcgatgagga aatggaagcg
    15001 cttgccggtt ggctggcttc ggtcgatccg gaaattccgt atcatgtctc tcgttttttc
    15061 ccgcattacc acatgccgga taaacccccg acaccggtga aaacagttta tcatctggcg
    15121 gaaatagccc gtaaacattt gaaatatgtc tatacgggga attgttagcg gatccgacaa
    15181 ttaagaaagg ggagaaaaat aatcgaaatt ttcctcccct ttcttttcat tttaaaaaga
    15241 tcaatatttt atgaattgag atccgtccgc accgcaaatc gggcattttc ccggacggac
    15301 tttttcgata ttgccgcaaa ccgggcaaag atagtagaag acttcttcgg tttcatccag
    15361 attatccaaa acttctttat agagttttgc atggacttcc tctgcctttt tcgccagcgt
    15421 aaaggttcca accgccgctt tattcccttc cgcctcggca tcctttaaga attccggata
    15481 catttccgta tattcatacg tttcgccggc gatggcatct ttcaggttat cggcggtact
    15541 gccgactttt ccggcaactt caaaatgtct taaagcatga attgtttcgg catctgccgc
    15601 cgcttgaaac aatttggcgg cattattttt tccttccgcc tttgctttac gagcataagc
    15661 ggtatattta cgatttgcct gagattcgcc tgcgaatccg gccatcagat cctgcattgt
    15721 tttactttcc atatgattct ccttattctt cattttgttg aattttttga ttcgtttcaa
    15781 ggcatttttt acagatccct ttgaaataga cacctttttg attgatttga aaacctttta
    15841 attcttccgg attaaatgaa tcaagcccga ccgtaaaatc atatatcctt ccgcattttt
    15901 cgcatttgaa atgcccatgc gtttcggtga caatgtcata acgattttca ttgtcctcga
    15961 tggtcagctc ttgaactaaa ttcgccttga ccaaagaatt caaagagtta taaactgtgg
    16021 tcttagacag tgacgggatt tcagcgatca accctgtata gatttcgtcg gcagtggggt
    16081 gagtacgatt ccgatttaaa taatccaaga tcttgacccg ttgataagac aaacgaatct
    16141 ttttctcctt caattctttt accaaatccc gatatgccgg ctcagtttcc acagaatctc
    16201 ctcacttata atataactat tcctaatttg taatcattac accttattat actcaaaact
    16261 gaatatcttt caagccctca tttcaagaaa attcggagac aaaatcatca aattttgaat
    16321 tattcaagca aaaaaagctt ccgaaggatt caaaagtatt ccgccatagt cccgcttcat
    16381 cacaaagagc ctccccatcc gcttgaatac tgttcctccc ccaattgcca cttgttgttt
    16441 tttcccaata ataagaaaaa tatgatctat ttcctgttta aaatgaattc gagatgattt
    16501 ccgctttttc cataaatttt catacaaaac acaaatcccg acgatttttt tgttattcgg
    16561 aaccggttta ttcttgtttt ttgaccgatt cggacctgca tttaaacggc ttatgcataa
    16621 tctgctcttt ttcagaatag caaatcgaaa aacaggtttg aacgcaaccg tgcattttac
    16681 gtctttttgt tgatggtaaa taccgaatat taagaaattc tgttcggatt gggtataatt
    16741 cgtttattct taatacatta ctcatatgaa aacggatcga agtccgagcg ataaaggaca
    16801 aaaatatctt taaacaggga gctgaaatga aaaaaaagat acaaattgta attccgatgg
    16861 caggatatgg ttcccgactt cgcccgctga cctggagcaa acccaaacca ttaatcaacc
    16921 tggccggacg aacttctttg gattatcttt tggatgaatt taccagtctt gaaaatgagt
    16981 ttgatcccga atatatcttt atcatttcac cgaacggttg gcaaattaaa gcctacatgg
    17041 acaaacatta tccgaacctg aaatgcaatt atgtggttca ggaagagatg aaaggtcaat
    17101 cgcatgctat tttcctggca aaggatttga tcagcggccc gatgattatg actttttcgg
    17161 atacattgct tgaaggcgat ctcggctttc ttgccgatga agacgcggat gggattgctt
    17221 gggttcaatg gaatcccgag cctcagcggt ttggcgtagc cattaccgat gaaaataacc
    17281 gcatcgaacg tttcattgaa aagccctcta ccgatgaata taaaatggtg atcatcggtt
    17341 tttattattt caaggaaggg caaaaattga tcgaagcgat cgaacaacaa atcaaagaga
    17401 acatttccct taaaaatgaa tattacatag ccgatgcgat taatatcatg atccaaaaag
    17461 gagccaaatt tacgatccat gagaccaaac tctggctgga tacgggtgta ccggaaactg
    17521 tcttctccac aaaccgccat ttcttggatc acgggcacga taataccgaa acagccatga
    17581 aacgcaaggg ggttgccatc gttcctccgg tatttatcgc agaggacgcg accgtcgaaa
    17641 gttctgtgat cggcccgtat gtttcgattg cttccggatg ccatattcaa aacagtgtga
    17701 ttgaaaattc gatcattgcg gagaatacga cgatcaccgg tgaagttata aaggattcgt
    17761 taatcggtga aaaagtcagt ttgaagagta cccccgccgc ttattttatc ggagataact
    17821 ccatcatcga gaaataacat ttaatatcct attttctgtc ccacatttca tatcccggag
    17881 ggatccaaaa tgtacaacaa tttatccaat cgagccaaaa cgatcaagcc gtccgccatc
    17941 cgtaaatatt ttgcggttcc ggacgatgta atttccttgg gaatcggcga accggatttc
    18001 gattctcccg cctgtgtcat cgaagccggt gttcacgccc ttcaatccgg gaaaacgcat
    18061 tacacagcta attccggcct caccgaattg agggaagcga tcagtgacga cttatatcgg
    18121 cgctaccgtg tgcgttatga tccgaacagt gaaattatag ccactgtcgg atgttccgaa
    18181 gctctttttc tggcactggc agctctaatc gatccgggag atgaagtgat cgtcatcact
    18241 ccctgttttg ttgcttatca ggcttccgtg ctgttggctt cgggtgttgt cgttgaggtt
    18301 ccttgtaaat tcgaaaataa ttttgacttg gatattgcgg cggtagaagc tgcgatcacc
    18361 cctaaaacga aagcgatttt attgggattc ccttgcaatc cgacgggtgc ggtcgcctcg
    18421 cgggaagaat tgcagaaact ttgtgatatt gcggtcgcac ataatctcgc cattgtgtcg
    18481 gatgaactat atgatcaatt ggtttatggg atagagcatg tttgtgtacc ctcattgaac
    18541 ggcggttttg agcgaacgat gctggtggga ggtttttcca agaattatgc aatgaccggg
    18601 tttcgaatcg gttatttggt ggcgcccgct ccgctgatgg acggcgctta taaaattcac
    18661 cagtatctga ttatgtcggc cccgacgatt gcacaatatg gagctctggc tgcgattgaa
    18721 aacggacaag aagatgcaaa acgcatgcac gatgaatatg atcgccggcg tcacttggtg
    18781 gtcgatggat tgcgcagcat cggtttgccg accgttgagc cgaaaggggc tttttacgtc
    18841 tttccgcaaa tttccggaac cggtttatcg agcgaagatt ttgccaaccg attattggaa
    18901 aaagagaaag tggcggttat ccccggcaac ggcttcggag cgggaggaga aggttttgtc
    18961 cgtatttcct atgctacttc ctatgaaaaa atcgaacagg cactggaaag aatgggtcgc
    19021 tttgtaaaat cgctgtaata ttttctatag cgctcctttt gcggacggtt tccgatggat
    19081 atcctcatat cgatcgaaaa ccgtccgttt ttctttaaca agttccatca agctttttaa
    19141 aacggttctt tgtcattcaa acaaccggaa ttgaatagac tactgataaa attgagggtc
    19201 gggggtatca tgtacggtta tgaatataca aatcatttga tcttaaacga acagatggta
    19261 aacctatggc aggaagagga cgggaagaaa gttgcggaag tctgggttct tcccggatac
    19321 ggagcaaatc tctgccgttt taaagttgac aatattgact atctttatcc cgctccgcaa
    19381 aaattagtgg acatccggca ttacgggacg ccggtacttt acccgacacc cgggattgtc
    19441 cgcggagaaa aatttacatt tgacggatcg ctataccggt ttccggcaaa tcggggaagt
    19501 atctttcgcc atggttttgc catggggcaa aaatttctat tttcggagcc aatcgtcagc
    19561 gaagacggag tcacgctgaa aacaagtctc ggaatatttc gcgatcatcc gctttattat
    19621 ctttttccaa tcgccaaccg actggatatc gattttacct tgaaaaatcg ctccgtcaga
    19681 atcgatatca cggtgactga tttggataca cagaaacggt tccctttcgg gttcggtttg
    19741 catccttatt ttaatctgat cggtccgcgg gaagaaaacc tgattcaggt accggtaaaa
    19801 aaatggttgg atcaggcgga aaatcaatta atcgatccga atgacagccc gattgatttt
    19861 cgctctccga caactgttcc ggaaacagcc atcgatgagg tttggacggg tttaacttcg
    19921 gaagcttcgc catcgataat ttatcaatcc gtcgggaagc gtttggttct tcatgcaacc
    19981 gacgacttta cgcattttgt cacttacgca cccgaaaacg ctccttatgt ttgtctggaa
    20041 aactggacct gctctccgga tgcgcataat ttatatgccg gtgaaaaaaa cgacgtcgct
    20101 cacttactga ttctgaatcc gggcgaagta tttaccggaa gtgtacaatt ttcaatcgaa
    20161 ccgctgaata attaaaaacc tatcttccga caaaaatatc cgattgatga atcggatatt
    20221 ttttattatt taaagcgctt tccgagtaat cagtgtcgca ttctttttgt aataaagaca
    20281 aaagcaagcg gcacataaaa tccaagaaat agggaaactg acgatcacca tccgaaggtc
    20341 gaaccagaaa tgggtcatga ttaataccca aacgatgcga aatattccga tacaaaaaat
    20401 cgtaatcacc atcggtgtgg ttgcgttgcc ggcaccccga atggtcccgc tgagcacttc
    20461 ggtcaggtta tgaatcggat aaaacggaat cagataaaag ataaataaaa ttcctgtttc
    20521 gatcaccgcc ggactttttg taaaccaacc taaaatcgga tagcggaaca agataacgat
    20581 aacggcaaga aaaatcgaaa aagaaaaggc catcattaat gcgttacgga tcgcccgctg
    20641 aattcgatcg aaccggcctg caccgacatt ttggcttaca aaggttgtta ccgccaatcc
    20701 catcgccgtc accggcgaaa acacaaagtt atcgatcttg aaataagcgg ctgatcccgc
    20761 catggcaacc gatccaaaca tgttgatata agattgaatc atcaaattcg caatcgaata
    20821 catggaagat tgaaatccgg cggggacact tacacggata atggaaccta acaccggcat
    20881 atcgatcttc attcgctgcc attcaagccg atccggaccg tccgatcgga ccagacgcca
    20941 catgacccaa acagcagaaa gaagttgaga aatcccggtt gcatatgcgg caccggccac
    21001 tcccattttc cagagcgtaa taaaaagata atccgcaaag atatgaaata tcccgcaaaa
    21061 caacaaaatc atcatcggtt cacgggaatt tccgattgac cgcataattc cggctccgac
    21121 gttataaatc aacatcggta tcattcccaa aaaatacagc cttaagtaaa cgatcgccaa
    21181 ttcatacact ccgtccggcg tattcataaa gcgtaacaga accggcgtaa gaatccaacc
    21241 cagcagcgtc aatgcgacac cgcagattgc ggctaccaat atagccgtat gttttgcttt
    21301 ctccatcctg acggcatcac cggcaccgta actgtttgcg atgacgactc cggctcccgc
    21361 cgataatccg aggaaaaaac cgaccaatag gttaaccaac gatccggtcg aaccgacggc
    21421 ggccaatgcc agatcaccga caaaattacc gacaaaaagg gaatcgataa ttccatagaa
    21481 ttgttgcaga atactgctta gaaataaggg gatcgcaaaa aaaaggagtt gcttccaaat
    21541 aattccttct gtcattgagc gattctcaga tttattcttc attccgcaac tttttatctg
    21601 cccttatctt taatcgattt cttttctgaa aacagcaaaa aaacaggaaa caaattcctt
    21661 tttgaattgt atcaggaacc ttcaattcct catgatttta tttattttct tccgatcgat
    21721 aggaccaaca aagctaagag aataaaaaaa catcctccga ttgattcatc gaaagatgtt
    21781 tgattttttc cgcaccgtgc ccggtttatc tcccctttgg gagacaaacc ttttcacttt
    21841 tttcctcagt tttcaaaatg ataattactg agaaaacagg caaaaattgc cacagcaata
    21901 acagaaatcg tagttaacat gatttccttc ctttccccaa taaatgggca ataaacaacc
    21961 gaaaaggagc tgctttcgtt gaaaagccga ttgtttcttt caaaggaaga gactgatttc
    22021 gttcctttgt ttctcctatt cggttttcaa ggtataaaat cgatatgact tttgtcatat
    22081 tattaatatt acatttgtca catcgctttc tatattacgc ttaatttcat cgattgtcaa
    22141 atgaaatgtc ataaaaaata tcagacaagc cgtcaaatcg agcctgtctg atatttatga
    22201 aaacaatttt tttacaaatt tcttacagaa aatccggatg atcccggcgg atgacactca
    22261 gtgcttcttc atcgccaatg acaattacct tgggatgaaa ttgtaaaatg gaagccggaa
    22321 cctgaggcct gacttttcca aacatcgctt catgcagaat ctccgctttc ttttgccccg
    22381 tgcataacaa taaaattttt cttgcgctca taattgttcc gattcccatg gttatggctt
    22441 gggtcgggac ttccctgata tcggtaaaaa agcgtttgtt tgcttcacgg gtactttccg
    22501 tcaaatcgac tacatgggta tattttggaa aataatcgga aggttcgttg aaaccgatat
    22561 gaccgttgag accgattccg agtaattgca aatcgatccc ccccatactt tcgatcattt
    22621 tttcatattc ccggcaggca ctctccaaat catcggccat accattcgga agatgtgtat
    22681 ttctgggatg aatgttaatt ttcgaaaaaa gatgatcata cataaattga tgataacttt
    22741 ggggatgatc agcaggtaag ccgatgtact catccaaatt caccgtccga actaatgaaa
    22801 aatctaactt tccctggcta tatctcaaaa caagttcact gtataacgaa agcggagtcg
    22861 acccggtcgc caatccgaac acacaagcgg gattttcctc cattgtttga gtaaagatgt
    22921 ctgcccccaa gcgaccggct tctaccgcat ctttcgcagg gataaatttt attttcatga
    22981 caaatcctcc aaaaattttg acctttaata agaattgatt gattatcatt cctaaaaggg
    23041 attaggataa agataaacaa ttgaccaaat cataacgcaa gaaagaatta ttgttaaatc
    23101 gtcaattgga atctataaat ttttgattcg gaaaggatct ttatgaaaga cttgctcaac
    23161 agtattcggg aacgcagaag tatttattcc atcgctaaaa aatcaccgat ttcggatgaa
    23221 agaatccggg aattggtttc tctggctgtc atgtatactc cttccgcttt taatatgcag
    23281 agtcagcgag cggttttgtt gctcgggaat caccatgaaa aattatggtc gataacaaag
    23341 gatactttac gcggcatcgt tcctccggaa caatttaaaa gcaccgaaaa aaagatcgat
    23401 ggttttgctg ccggatatgg gtcaattctt ttttttgacg ataccgaaac ggtagccgat
    23461 tttcaacata aatttccgct ttatgcggac aatttttccg tttggaccga acactccaac
    23521 ggcatgctgc aatatattgc ctggacgctg ttggaagcgg aaggattcgg tgcctctttg
    23581 caacattaca acccgttgat tgatgaaaaa gtaaaatccg catgggacat tccggcatct
    23641 tggaaactca ttgcgcaaat gccgtttgga ttgccgacgg ctccggccga taaaaaagaa
    23701 tttgttccga ttgaaaaaag atttaaagca tttgagtaaa taaaatttat tttgatatca
    23761 taatactaat ttctcataaa ataacggaga gaatcaaaat aaaagtcgga agcgattaga
    23821 attaaagcca aaaattagaa ggaaatccgt tttagatgaa aacgaatatt gaaatcattc
    23881 atccgctgcc tcccgaaaca tacatacgtc atgctctgtt tgactttaat ggtacttttt
    23941 cactgattcg tgaaggttgg caggaaatta tgattccgat gatggttgat ttactgatgc
    24001 agacgccgga acatgaagag gaagccgaag tcacgcgggt ggtaaaggaa tatgtcactc
    24061 aattaaccgg aaaacaaacc atttacctga tgatgcagct ggccgaggaa atcaacaaac
    24121 gtggcggaac agccgagtct cccgatgttt ataaggatat ttatcaaacc aaattactgg
    24181 aacggattca gtatcgccgt gaagggctgc ggaacggcaa tatccggccc gaggaatatt
    24241 tgatccccgg atccgtagaa attcttcaag cccttaaatc ccgaggtgtc aaatgctatc
    24301 tggcatccga aatggatgaa aaatatgtgt tggaagacgc tgacctactc caattaaccc
    24361 cttattttga tgaaatccat ggcacgttgg atgattatgt taaagtttcc aaaaaaatga
    24421 tcatcaaaga aataattctc gacaacaacc taaccggtcc ggaatttgcc ggaacgggag
    24481 atggttttgt tgaaattgaa aacaccaagg cggtgaacgg aattgccatc ggggtcgcac
    24541 cggatgaaaa agatcatacg aaagttgacc catggaagcg tgaacggttg attcaagccg
    24601 gagcggattt gattattcct aattttaccg ataccggggg gttgattgat ttactttttc
    24661 aaggaaaatc gaaaatcttc tgattgatcg atcagaatag gattaaaaag tcgacaacgc
    24721 atcccgatca atgattggga tgtgcttatt ttataaggat tcgatcattt caaaccggac
    24781 ctgataaaca atctcttgtc cgggatgaat caatttcaac cggttttcac gccgattttt
    24841 tgcccgaccg tcaagccggc tgtttgcggg ttcgagtccc aatacatatt cttttacacc
    24901 catcattttc cactcaataa attcatccaa agaagcggga tcaaaatata tcgccactcc
    24961 tttttgatac ttaggctgaa aaacttttac gggtttagct tgatcgaaat gatgataata
    25021 gcacatttca acaaatccgg gctgtggagg gatcatttta ttccactcgg ctaatccttc
    25081 gtttgcatgc tcatctctcg gtgtaaccgt tgtcgaagga atatcgacaa tcaaatcttc
    25141 atccagcagc ggatagccga tgttgatatg atataaaatc atcaggggtg aaattttatc
    25201 tccgatattt tcgatcctat cggtaagcgt aatcctattt tcattcttcg agcagacaat
    25261 ttgtcgattt tggattagtt tttctccaaa aaacatggaa tgattcaagc gcgcgcagat
    25321 ttgtatttca tcctgatctt cttcccacca aatatgttct gcgggagtat tatgtgctct
    25381 tccgtgctgt cccaatattt ctccgtcatc ttccgaagga attccgatat tggtcaaacc
    25441 gcaagtggct aaaaatccca aattcgtcgt cttaatccat ccgttgccgc ggtcatcata
    25501 ataagccggt gaacaatatc cccctaccgg gaaataaccg taattcaaac cctgaaatga
    25561 taatcttgaa atgtccgcgc ctcgatctgc cgaaaccgta aattccaggc ccaatccgtt
    25621 tttcacctga aacaagcgga ttccgtcacc tttgccgccc accaatcgat gttcttccac
    25681 accgctaatc tgcgaaggat gcccgaagta agcttctttt ttatccatgg taacctctga
    25741 ccgattcctt ttcaatgtct gttttcaaaa cgcatgaaat aatcatccag ttcagaacta
    25801 agttctattc caactttttc gaaacaagta agaagaattt cataagtccc ttctccacct
    25861 aaaaaatccc aaaattcgga ggctaccttg atttcattgt ccaaatcaat cattccattt
    25921 attgtccatc gtcgatatgg cttcggttca taaggattat aaggaattgc aataagggta
    25981 tttacttttg catatggatt ttgggctaat gtaacaccaa cccaatttaa caaggtacgt
    26041 ttatagcttc tgaaatcatt cttattcggt ttcgcagttt ttaaatcaat taaaaataat
    26101 tcatcatcaa atgttgaaag aaacaaatca actttaattg ggtttatatt tataaattga
    26161 ccagattggc aaactctacg tatttcttca atttcagctt ttttatcagg aattcgttcg
    26221 ccattttcca attggttaat aatctgttgt ataacgctct gagattcact gctaattaga
    26281 cttcccgaag taaattgtga ttgaacaact ttaaaattat cttttgcaat tgattttgca
    26341 ataggttcaa aaattgaaat gccaaaagtt gtattgagag actgtataaa tgaaaataaa
    26401 gccatcctat cttttcccaa taatcgagta tgaaaaggca ttgaaatggt ttccggctta
    26461 tagtttcgta ttttgtcacg aagacagtta attaatatat gctcaacatt ttctacctgt
    26521 tgattattta acgacatgtc ataaatcctt tttaatatga aagattactt ccgaataagc
    26581 tcctttatct ctctcagttc gatttaatac aggacgctta tatcgtttta taattttcat
    26641 tcctgattta ttaactattt ggggatacaa atcatattta tcattcgcaa ctaaaaatat
    26701 atcataatcg ttcgataaat atttttcaca attaatcaga acttctgaaa ttccttcaac
    26761 atacgattgt ttagccgatt caccttgtcc tttatataga ggaccaatct ctaaactatc
    26821 atgtcgatca aaaccaaaaa gatcatatgc ataagcatgt tgctcatgat aatctatcat
    26881 tcctacataa ggtggactag aaaaaattcc tttaattttt tgttctgaaa tctttttgcc
    26941 gaaattagga tttagtttca tcacttcatc aataatattt atattcctgc tatctccaac
    27001 taatgatact tgcattgtat cagttcgaat tgtatcgaat tcagcaagtc tcttgatcga
    27061 atcttttgta tatgtatccc accattttaa aatagagaaa agtggtttac agattttact
    27121 gtgtttagga caataataag ttgttgtttg tggttctttt aaagttgcaa gatctgaatg
    27181 ggtagtcgct ctacatgaac gcattgtcct gcttaatatc aatgaaagaa tttcttttgt
    27241 cgattgatca ttaatttttt taatctcatc atatacaaaa tatatctctt ttttaactga
    27301 agggaaaaac catctatcga taaagtttcc agtttcaggg aattctatat caatctggta
    27361 cttagtaatg aggtcataat actttggtaa aaaaattaat tctttttcgt gtccatatac
    27421 atctggatta atatctctct ttaagacttg atatttatat tcgggtgaag gaaaatattt
    27481 cgaattaaat atatttaatt cttccaaaag agctgcttca aattctgcga tttttgaata
    27541 atatatgaat tccttcaagg catttgtaat tcgtctaatt tcagaataaa cagacggtaa
    27601 atcatatttt tgaattttgc atttggaaat aagactatta aaaatagata tatctatccc
    27661 gattgcattt attcctaatt cgtttgcttc aactaaggta gtgccacttc cacaaaaagg
    27721 atctaatacg atatctcccg gccggaaaac cacttctgtt ttaaatttgt caacatgatc
    27781 atccaaaaaa tattcaacta attgaggtat gaatttccct ttatagggat gcaagcgatg
    27841 aacatgctta gtggtctcac tttcctttac atgatcgaat gaaagagccc aatttaaatc
    27901 ttctcctaat ttagatttcc agtcaatttc tcttttcccg ttaaaagaat cataatagtt
    27961 ctttaaatca tctttttgaa tttttgtgtt ctcaccatca aatatttttt ttaccttacc
    28021 atattgaatt aaataagaga tgtttgaagg agagactttt ttctttaagt atttcgttgc
    28081 ccagtcacta gcttcattaa tagttaaaag ttcagtgttg ttcattttac tcatcctatt
    28141 aaattttcag tcaatattga ttatatccgt aattttattg ggaaccgtta atctcaaacc
    28201 gcctaaatca tataattaga tttatttttc ctaatcagaa tttcagaaaa tacaataacg
    28261 agtaaaattt gaaagtgcat caaaatagga tgaaaagact gtctcaacga cagtctttca
    28321 aatgccgata atctttgttt cgttttaaat aaataccgaa aagaaagcga atcaatcaca
    28381 gacaaatcga aaagaggaat cgtttttaat aatgaaatcg gaaattgaaa ttatcaaacc
    28441 cttaccggac aaaatcgaca tccaccatgc cctttttgat tttgacggga cactctcgtt
    28501 aatccgcgaa ggctggcagc agattatgat ccccatgatg gtcgatttta tcatgcagac
    28561 accgaaccat gaggataaag cggaagtaca acggattgta aaagaatatg tcacccgatt
    28621 aaccgggaaa cagacaatct ttcaaatgat acaacttgcg gaagaggtcg agaagcgcgg
    28681 cggaaaagct ttggatccgc gtgagtacaa agagatatat catcaaaaac ttttggaaag
    28741 aattcatgat cgtctggaag gcttaagaaa cggcaccttt caaccggaag aataccttgt
    28801 tccgggttca ttttcgatgc tgcaagcatt aaaggccaga ggtgtaaaat gttatctggc
    28861 atccggtacc gatgagaaat atgtgttgga tgaagcgcaa ttactgcgtt taacccctta
    28921 tttcgaagga atatacggtg cccaggacga ttacattcgt ttttcgaaac ggatggtaat
    28981 ccagcgtata attcgggaaa ataagctgag cggacccgaa tttgtcgggt tcggcgatgg
    29041 atttgtcgaa atcgaagata ccaaatcggt cgacggcacg gcagtcggcg tcgccagcaa
    29101 tgaacaaaca cgcgaaggaa ttgacgaatg gaaacgggaa cgcttaattc aggcgggtgc
    29161 ggatttgatt attcccgatt ttcgctgttc cgatgaatta ataaaaatat tatttccgaa
    29221 agaaagcaga taaggaaggg ttcttatggc ttataaaact tttgatcgtt ccaaattaaa
    29281 cattaaaccg atctccgagc gaattcatga tcttcatttg ccgcaaatgt taattccctt
    29341 agacgcacct gtccctccgt tttcggatcc tgcgattccg aaaattgcac aggcaatgat
    29401 caaagcgcat gaaaacaacg ccccggttat tctgatgatg ggcgcacatt tacttcggaa
    29461 ggggatggca ccgatgatca ttgatctgat ggaaaaaggg ctgatcaatc atatcggatt
    29521 gaacggagcc ggttgtattc atgattatga attggctcga atcggagcga caaccgaaag
    29581 tgtcgcccgt tatattcatg aaggacaatt tggcctgtgg caggaaaccg gcgaactgaa
    29641 tgacattatc aatcacggtt cggatgccgg attgggcatc ggcgaagcgg tcggaaaagc
    29701 aatcgttgaa aacgactatc cctatcgaaa aaccagtttg ctggcccgcg cctgggaact
    29761 cggagttccg gtgaccgtcc atgtcagcat cggttcggat attattcatg aacatcccaa
    29821 ttgtgacggg gcaaaattag gaaaagcttc ctatcaggac tttctggtct ttaccaatac
    29881 catcgatcat ctcgaacagg gcgtatatct cagttacggt accgccatca tgggacccga
    29941 ggtctattta aaagccttgg caatggctcg gaatgtcgcc tttcaagaag gacgacaaat
    30001 ctgccacttt accaccgctg tttttgattt acaggattta ggcaccgact ggcaccggga
    30061 gatttcaaaa gaccaagctg cttattactt ccgtccctat aaaactattt tagtgagaac
    30121 cgttgcggat ggcggtgtaa gctactatgt acggggagat cacgccgtga ctttcccgaa
    30181 cctctatcat tcaatcatcg atcactttca ggacggccgc gaatgaattc gaaacggatt
    30241 gccgaaatat taaaaaatta tcccgatttg cacattctgg ttgcgggaga ttattttctg
    30301 gatcaatatt tgatgattaa ccgcgatctg gcggaaatat ccttggaaac cggtttggaa
    30361 tgccatcagg tcacagagat tcgcaacagt ccgggggctg ccggaaccgt ggcaaataat
    30421 cttgtcgcat tggatgtttc cgtcaatgct ctcagcgcaa tcggtaagga cggccgcggt
    30481 tttgatttaa aaaccgaatt actgaaacgg aacatagata cccgcgctct gatcgaatcc
    30541 ccgctgatta tgacccccac atataccaag ccgatggttc aggggaaaaa tgaacccctc
    30601 catgaactgg aacggatcga tataaaaaat cgttctcatc ttccgattga aatcgaagac
    30661 acactcattg agaatcttga aaattctttt caacttgccg atggaatcgt gcttgtcgat
    30721 caggttcagg aagaaaattg cggcgtctta acggatcggg tcagaaatca aatcgcagct
    30781 ctggcattaa aatatcccga caaacctgtt ctggcagatt cgcgcctgcg aagcgcggct
    30841 tttaaaaacg taacggtaaa atgcaatcta tccgaagcgt tgaatgttac cggcgaatcc
    30901 gacgttgagc gggctgccaa aaaaatggtc gccggcggcg ctcgcagcgc ttttattacg
    30961 atgggagaaa agggtatttt tgcctattca aacggaaaag gtgtgaccgt tccggcggtc
    31021 agagttgagg gacctatcga tatatgcggt gccggggaca gcgccgccgc cggaattctg
    31081 agtgcgttat gtgccggagc aagctttgaa gaagcagcaa ttatcggtaa tctgacggca
    31141 tccataacaa tcctgcagat cggcactacc ggtacagcca gtcgagaagg gattgttcgg
    31201 cgcgcatttg aattcttcgg cgaataagtc tttttcagcc ggcaagttcg gataattttg
    31261 cggctcccaa caaagtttcg gtccctttga ggtgagacat tcgaattttg gtcttccctc
    31321 gcatttcggt ggggagaaat tcgggtatag agtgagtcaa aattggttcc agtaaatccc
    31381 agctattcac cgccactccg ccgccgatga caatctcttc caaatcgaat aaaacaatgg
    31441 cattcgaaag cgataaggca aaacatctga atgcataatc gacgatttcg gatgcggaag
    31501 agtctcctct tctggccgct tcgatcaacg gctttgccga tatttcgccc atcttcgaga
    31561 cttgttcacg aaacacagag catgcttccg gactttcgat cagcgctttg gcatcccgtg
    31621 ccaaagccca gccggatgca acagcttcga tacatccttt gctgccgcag gcacagggga
    31681 acgcggcttc acccacatac tgcactttct ggtgaccgac ttctccggcc attccctgtc
    31741 cttgaaggat ttctccgttc aagatgaagc cacccccgat tccggtgctg atctgaatat
    31801 acatgaaata atggggtttt ccttggctgc catagaacca ttcgcccaat gcagccacat
    31861 tcccgtcatt tcccattcga aaagggaacc cgaaccggtc ggcggcaact tccgccagcg
    31921 ggtaattctc ccagccttta atgtgcatag accggattac atgcttgccg tcgggattta
    31981 ccggtccgcc gaaactgatt cctcctcggg taattttttc ctgcggaata ccggattctt
    32041 ttagactctt acccgccgta ttcagaatca gctctaaact ggcttgtgca cccgcatccg
    32101 cgggcgtcct cgccttttgg taaccaaaca gcctgccgtc gggaacagag actaccgcgg
    32161 ttgccagttt ggtcccgcca aagtcgagcg ccagagcata ttcgggtttt gtcatcgctt
    32221 tccttccagt tcgtgcatcg ctatttcacg caggaccatg gtcagtaaat gatcacatac
    32281 catatgtaag tcttcgaccc gctcaatgct atccgtatca atgttgatcg gtaaatccac
    32341 catggagatc atttttcctc ccttgaatcc ggataaaccg attgttttta agccgatttc
    32401 tcttgcggtt tccaaaccgc gaaggatatt ggcagagtta ccgcttccgc tgatggcaat
    32461 caaaatatcg cccggctcgg ccagagcttt caacggttct gaaaatactt tttcataacc
    32521 ttcatcattt gcaaaagccg ttaaagtcgg agtgctatcg ctgagacaca ttgtgcgaat
    32581 cgccggaagg tatggaacac gagtgttttt cgaaaaatcg cacgccatat ggctggcggt
    32641 tgccgaactg ccgccgtttc ccatcgtaaa aatcatcttc cgttccaatc gagccgaatg
    32701 aatcatttcg gcggctcgta cgatttgatc gaccggcatt ttatcaatcg cctgattcaa
    32761 aaggccaata tattcattga cccattcttg ttttgtgctg ttcataatta tttctcatcc
    32821 tcttattgac atcaaaattt cattcggctt tccggttttc gggaacggat ttattcctgc
    32881 ccgaaatgcc gttccaacat tttgcataag gtatgataga tcagaatatg caggttctga
    32941 atttcatccg tctgcgattg attcacagca attaagatat cggcatcttt tcgcatcttc
    33001 ccgccatccg atccggtcat acaaatcaca taaactccca gttcctttgc caataatgcc
    33061 gcataatgca aattttcggc gtttccactg gtgctgattg agatcattac gtctcccggc
    33121 ttggcattcg cgtaaagttc ctgagctacg gcaagattcg gatgctgaaa atcattttcg
    33181 atcgcagacc gtaaaaccgg attcaagcct aagaccatca cccgttgcgc ttcttcaaga
    33241 ttttccgcta aaacggaacc ctcttcggac tttttcaacc gagctgcaag agccgccggc
    33301 atgtgccttt ttacaacaaa ggctttggaa agttctccgc tgatatgcag cgcatccgcc
    33361 atactgccgc cgtttccggc aacaaacagg gttcctccgc aggaatacga ccgtcggatc
    33421 aactgatagg cttgagcaat cggttctttg acattttcaa tttgaggatg cagcctgatc
    33481 gcttcatcta aaatcaaccg tacagacgga tcaatcattt ccgtcatcgg cagagaaatt
    33541 ctctttgatt ccacgggaaa ggtctcgcca tttgccgtca ataattgata taggttcggc
    33601 aaagtgacct tatgatttcc gcaaatatgg aatccttttc cgcccaacat taccggtcga
    33661 ttcaccacat tcttgcgcgg acgatagtaa tactgaactt tatcttctcc cagaggagca
    33721 cgataatcgc ccagatcgat cagatcaaaa tttgcggtgg taaatacttt cagcggattc
    33781 cccagattcc gtgctatgga aagggctttc agaaaaactt ccggaccgat taccgccgaa
    33841 ccgacattca gaaatactcc gccttccaaa tcacaaaccg atttgcagaa aattttaaaa
    33901 tcctgacccg aaccccatcc gatagccgca aaatccaccg ccggatgttg atgaataata
    33961 tccgtcccga cagccacatg aacggtatag ggaattccca aacgccaagt atggtaaagg
    34021 acactgacat cgcgatattg ggcaaatcga tcatcagttc cgatcaactc acccaaagac
    34081 tcaccgattc ccatccctcg tttggcgccg ttttgaatcg cggtattcat cagcaaaccc
    34141 gtctcctcgg ccatcccgaa tgtgccgtcc ttaatggaat ccgctacatc ttcgcttgta
    34201 tgtcccaaca gcgcgatttc aaaatcatgg attgttgctg ctccgttgct cgcaacatgt
    34261 tgcacatacc ccttttccaa aagatcgatc aaaatcggtg ccagaccgga tttgatcaca
    34321 tgcgcacctt gcatccaaat taagggacga cccgcccggc gggctgccaa aattctttcg
    34381 gcgacttctt ttaaatcggg agaatcaaat tccgcaacag gtgtcgacgg aaaaatgaag
    34441 tcatcggtcg caactcgatt atgacgtgcc ggaaggggca ctgtacggac tttctttaga
    34501 tcaactgcag gataaggcat atatgtttct ccgtgggata tcgaacaaaa acgatatcat
    34561 aaattttttc tttcttcaaa agtatcaatt atctatcgaa gcgaaatcga ttcttttcat
    34621 tgagcttttc aatcttcctc tataaaaaga atttttattc gctgatatta ttggaaaagc
    34681 atatcattaa aacaaagaac ccggcaaaat tgccgggctc tttatgttcg aaaaacagat
    34741 tatttgactt cgtccaaatt atctttggtt acaacggtta caccggtgtc gacaacaaac
    34801 gcttcttcat cagcgacttt ctcaccgttg atgagtttcc aagcggcttt cacaccttcg
    34861 ttacccatga cccacggatt ttgaaccatt gtcgcctgga tcacgccacg ggataccaga
    34921 tcctttactt catcggaaaa gtcaaagcct accactttaa tgacaccggc ttttccggcc
    34981 tgatcaaccg cgttaccggt accgacagtg gagccttcgt tacaagcata aaaaccggcc
    35041 aggttcggat tcttcatcat gaaatcttcc gcatagttca aagccttctg cttgtcaccg
    35101 tcgcagtatt ggacaccgac gatcttaata tccgggaatt tctcggcaat ttccttgcgg
    35161 aattcggatt ctcgagtcat ggttgtctgg gatccggcct gagcgttgat gatggcgatt
    35221 tcgccctttc cgtcaaccaa tttgcccagt tcatccgctg ctaagcgagc ggcggcaccg
    35281 ttatccgttt ggtaaatcac atggaatttt ttggtattcg ccagggtatc aatcaaaatc
    35341 atcgggatgc cggcatcata tacttcttca attgccggaa ccaatgcttc tgcatccatc
    35401 ggagcgagca gaatggcatc ggctttgttg gtgatttggt tttctaccat attagcctga
    35461 accaccgagt ccactttggt tgcgggagca tcaaacacta atttaacgtc atggccttct
    35521 tttaccaaat cggcaaccgc agcttcggca ccggccttca ctttcagcca gtgctgatcc
    35581 atggaatcca tggtgatcaa tgcgatggta tagctctttt cggcagccat tgcaacactg
    35641 caaacagaga gtaacatcgc aagtacaata attaatgtaa cttttttcat tctttccctc
    35701 ctaatatatt tttgaaaact gcaaaatgca gtttatctac tactattcta atcttctctc
    35761 ttaaatcggt ctataaaaac agcggcgata atcacgattc cgattatcat cttttgaata
    35821 aaaggagaag cacccgacag gtttaaaccg ttccgtaaag ttgcgatcac caaagctccc
    35881 aaaaccgtcc cgggaataat cccttccccg ccgagagtac tggctccgcc gatgacggaa
    35941 gacgctaccg catccaattc atagccgtca cccgccgtcg gagcagcgga aataacgttg
    36001 gcagtcataa tcataccggc ccaagctgcc aaaaatccgc tgatggtata aacgataatc
    36061 aacgtcagat tgacgttcac accggataag cgggtggctt catagttcga acccatcgca
    36121 taggtatagc gtccgatttt ggttttcgac aaaacaaaac caaaaataat cgccaaaatc
    36181 gccataatat aaacctgatt cgggacgtta aacgtccgtt gcgttccgat ttgggtaaat
    36241 tctttcggta agctcccgat cggaattccc tgcgtcagga ataacgccaa gccccggaca
    36301 gtcgtcatcg tacccatggt ggcaataaat gccgggagtt ttccatagac aatcaacgaa
    36361 ccgtttatta caccgaccaa gacaccaaaa agtaagccgg cgaccatcga aatccagacc
    36421 ggcattccgt cccgcatgaa aattgccgca acaacacccg aacaggcaat caccgaaccg
    36481 attgacaaat caatgccggc ggtgatcaaa acaaaagtct gcccgatggc aatgataata
    36541 atcggagtgg cttgctttag aatattcagg atattcatca tggaaataaa atttcggttc
    36601 gctaatgtaa agaaaaggaa cagcattagc aaagctgcca gggtactgag aatctgcctc
    36661 ttttgacgtg acattttcgc tttttgcttg gttggaactt ctactgttgc ttggctcata
    36721 atttacctca tctacatgta ttgagtagca tattgcatga taatttcttg atttgcttca
    36781 tcagcgtcta attctgcggt aaccctgcct tcacacataa ccacaatccg atcagccatt
    36841 cccaaaattt caggaagttc ggaagagaca aaaaccacgc caattccctg ctctttcaat
    36901 tgattcataa ggttgtaaat atcaaccttc gctccgacat caatcccccg cgtcggttcg
    36961 tcaaacaaaa tcacatgagc cggtttcacc agccattttc cgaccactat tttttgttga
    37021 tttccgccgc tgagattctt aacgatctga aataccgaag gagtgtgaat ggtcaattgt
    37081 ttaaccatat tttcagcaat cattatttct ttttttgtat tgacaatgcc gcgatgtgta
    37141 accttttcca tattcggcag cgtagcattt tgatagaccg gcatggaaat gcatagtccg
    37201 tcccgttttc ggtcttccgg aacgcagaaa attcccgttt cgattgcttc ttgaggttta
    37261 tcgatcctga tttcttgacc gtttagaatc aactgaccgg aatctttttt atctgcaccg
    37321 aaaattgccc gaaccgtctc tgttcggccc gcacccatca aaccggcaat ccccaaaatt
    37381 tcgccttccc gaacagtgaa actgacatct cggacagctt tccccgcaga gagattttta
    37441 acttcaaaaa tggtttttcc gaccggcatt tgaatccgcg ggaatttttc ttttaattcc
    37501 cggccgacca tataacggat aatatcatcg gcggaaagat tcttgaaatc cgttgtaatg
    37561 atatgttgcc cgtcccggat gatcgtaatg cgatcggtaa tcgcattgag ttcttccaaa
    37621 cgatgggtaa tatagatgat tcccttccct tgctttctca aaactttgat aatactgaat
    37681 aaatcatcga tttctttgat cgtcaaagcg gaagtcggct catccatgat tactacattc
    37741 gcatttgcgg aaatagcttt ggcaatttcc accatctgtt gtttggagac agtcaattcg
    37801 gagacacgag aattcggatc cagattgact tccaaccggt ctaaaatctt ttgtgcctct
    37861 tcaatttgat gttttttatc gagaaaaatc ccgttttgaa attccctgcc taaaaagata
    37921 ttctcagcca cagtcagatg acgacataaa ttcagttcct gatggatgat tgcaattccg
    37981 gcatcctgag catcgcgcgg cttttgaaaa ttcatttcct ttccgttgac aatcaccttt
    38041 ccttcgtctt tcatataaac gccggaaaga atcttcatca gggttgattt gccggaaccg
    38101 ttttcaccga tcagtgcatg cacttcaccg ggtcttaaat ccaaactgac atgatcaagc
    38161 gcttttacgc ccgggaatgt tttggatatt tccactaact gaactaagag atcatccata
    38221 accggtttcc cctttcagga attgaagtcg atattcaaaa gactagtttg tgagtataaa
    38281 ctaaaatttg tcagttgtac cgacaaatct gttcttaaag aaatttgtca tttcatctca
    38341 ggatttttat catatcataa ttcgataact atgtaaattc tttttatcaa agtattttcg
    38401 cttgtttgct ctttcgaccc taagctccct gccggtagaa tattttttat tgcagcagtc
    38461 gccggtaatt gaagcattcc gaaccactta caggatagga aaatattgaa aatcctttgc
    38521 tgttcgaagc cgtaaattcc gagtttccgt aaaacgggaa gaaaatgccg caagagatcc
    38581 gaaaaaaacg aaaaagattc ctgtgatgag caaattatgc cgggaatatc agatgctttc
    38641 aaccacaaga ttcatcctta gaatagaaac tgttcgccgg tttcacgaaa ggaattctat
    38701 attcggcaca ggaaagcaaa ataagtccgg tatttgggaa accggtgatg aaattcgata
    38761 tagtgataca attcgaaatc agtttacgga aaaataatca ttttaaacgg attcaacatt
    38821 caattgctcg ggaatttact tttttaaccg aaatgagacc cgaaggaatg cctttcaatc
    38881 aaattatcgc ggcaatgatg ctaccatcaa agacttccct ttccccgatc caaaataatg
    38941 tctcattgtt tatggttaat ttctatattt tatggtgctt ttgacgttcc gctgctctta
    39001 tggcgatgca tcacattctt gacacagatc cggcgaaaaa gatgtgccag aggaaatgaa
    39061 aatggaaaaa gaagaccggt ttgaaaacgg aataccgaca agttctttac taaacagact
    39121 ttttaaatca tccgatttaa atgatttcct caaagaaaac gaaaaagaga tggttatccc
    39181 gacatttatg gaacatctcg acagactttg ccgggaacga aatgtcatca gagagcatgt
    39241 aatcaatcgt tccggaattg aacgttgttt cgggcatcaa ctctttcgtg gtaccaggaa
    39301 gccttcccgg gataatgtgc ttcgtttagc ctttggtttt gggctgaatg ttgaagaaac
    39361 acaggaactt ttgcgctcgg ccaaaaaagc tcctctctat cctcgaatca aacgggatgc
    39421 cgctattatt ttcggacttt ctcaccaaca gacaattatg gaaatacagt ctgtattgaa
    39481 tgatttggag ctttcgttgt taggtgaaga aaaatatgaa cataccgagc gctgatcaga
    39541 tcctcgaatt cgatgattta aaatatccgc aggattttct tgatcaatat gagctactgg
    39601 aatgtatcgc acaaaaaacg gaaagtgaaa ctttttttgt aaaaaacaga caatccggag
    39661 aattcgccgt cgcaaaatgc tatatcggga aagaattgat atccgaaccg accgaaggtc
    39721 agatattgaa tttgcttcac cataacggtc tcccccgatt catcgaagaa tataaaaatg
    39781 atgagacaat atgcgttgtc cgcgaatata tacaagggat ctctctgtct gagcgaatga
    39841 aggacggttc gctcacggaa cgggatgcga ttccgattct tttacagctc tgcgatattt
    39901 tgacatactt gcatcatcaa tgtcccccaa ttattcatag agatataaag cctgaaaata
    39961 ttattcttgc cgaagacggt tccgtcaagc tgatcgactt tggtatcgca cggacttatg
    40021 acaaatccga aaaaaaggat acgatcattt tcggaacaca gggttatgct cctccggagc
    40081 agtacggttt ctcacaaaca gattgccggt ccgatttgtt ttcgctggga atcatcatcg
    40141 ggtatttatt aaccggaaaa acggatcgcg aagctgtagc aaaaactcta acaaacaaga
    40201 ggattttgca tatctatcaa aaatgtacgg atttttcacc caaaaggcgt tactcctcgg
    40261 cgactaatct taaacgggct cttcttcgct cggacggtag aaggcaaaaa acttttttcc
    40321 gttttgttaa aatcgctttg ttttctgttt tgtttttcgg tttagggttg attgcgggtc
    40381 gattcaccga ttttcctgcc tttccggctg ctgaagtgac attcgacgaa ccgctgatag
    40441 aaaaagcggt tcgtcttcag ataaacagag cggatgacct acccattaca aaagatgatt
    40501 tgctttctgt taccgaattg tatattttcg gcaacagcgt aattgcgaaa acagaagatg
    40561 aaatgaaccg gtcagcggaa ctgctctttc aggaaggccg gatggagcct ggttcggttg
    40621 tgtccctcag agatttaaat aaattgccca atttaagaaa agtcgctgtt tcaatgcagc
    40681 ggattaaaga tttgaccccg ctcgcaaatc taaaacaact ccaaattctt attctgaaaa
    40741 acaatccgat agaagatctc tctccattga atgggctgga tagtttggaa aggttgtccg
    40801 tgtttaatac ctctgtcaaa gatttttctc cgcttgcgga atgtattcgc ttgagagatc
    40861 tggatgccgg agatacgctt gttcggaccc caatcgcctt tgcgccagcg aaaaacctga
    40921 cccatctaaa tctttataaa ttacagatgg atacattggc gggggtcgaa tctcttcgga
    40981 atctggaatt tatcgagatc agcgatgttg ccgacgacga tctttcccct cttttatccc
    41041 tgaaaaagtt gaaaacagtc aaattaagcg agaagctacg tcaagccgca gaaacgatcg
    41101 caaatgcggc acaattcgaa attgtttatc gctgagagat caatcaagcc aaataagtga
    41161 gcaatttgtt caccaatttt attttctcct ttgttagact gaaactgtca atgacaatat
    41221 gtcaatttta ccgaacaaag aggtctgaaa aatggaaaaa agaactgcaa agtcgctcat
    41281 tattatcttc atatttatat tatgtctggg cataaaggtc ccggcattag ccggatcccc
    41341 ttcggatgcg gagagtcaag ctgaagcatt gaagcagctc ggtctaatga aagatgaccc
    41401 ggaactgtca ttttcatttg atgaggaatt ggatcgcgtc caagccacgg tctttcttat
    41461 acgatcgacc ggaaaagatg ccgaagcaat atacggcaaa ggctcgcatc ctttttcaga
    41521 tgtcccggcg tgggctgata aatatatcgc ttatgcctat gaaaagggtc tggtaaacgg
    41581 agaatccgaa acgaccttcg gaaccgaaaa agcggattat cccatggttc ttacgtttct
    41641 actgcgatcg ttgggatata acgattcgga aggtgatttt tctataaagt caccggaaaa
    41701 tcttgccgat gccgtcggac tattgcccga tgatatcaat gaggatgaat gccgttatgg
    41761 agaccttgtt gcggtctcat gggctgcttt gcaagcggat ctcaagaaag ggaatcaaat
    41821 gcttgccgaa aaattaatct cggaaaaccg gttcaccgca agtgattatc aaaaagcact
    41881 tgaatctgcg aatacaaacg ccgcggaaac cgtttctgtc tcttcttttg aaacattaaa
    41941 gtccgtgctg ctaaatccgg atgtccgatc ggttaccgtc gattcgatcg gcaccccatt
    42001 aataatcacc ggcgaattga caattccgaa aggtgtaacg gtgacgataa accggggaaa
    42061 tgatttttat atcgaaaata atttaatcaa caacggaaca atcgaggtaa cgggagcaga
    42121 ttccgtttct ccgaatttta taaactattc cgtgatggcg atacaaaagg gtggcaaggt
    42181 aacgaataac ggaaaaatag tgctccagcc ttccgtcctc gcagatacgg aagatcgcgg
    42241 accggtcgga ggccaattac gtatcttcga cggcactttc gaaaataacg gctctgtcta
    42301 tctgaaagcc ggaaaagtga acactcacgg cggaatggta gcagtcattg aggaaccctt
    42361 taccaacaac gcattgatca tcgtagacgg attccaaata gatatagccg gaacgtttac
    42421 aaataatgcg gatggaatca ttatcaacaa cacgtatatt tccacggcaa aaggcggcac
    42481 gttcataaat aacggctcgc ttttaggtga aaaaatagtt gaataagcaa attcggccgg
    42541 taaccgtctt cccaagggaa aaaacggaat gagtttttcc gtcgtttcat cgaaagatca
    42601 gccgaaggtg gaaaataaag atcatggcac cgataaaggg atctaaagaa tacgtgaaaa
    42661 gaaataaaga gcccgtaaaa acaaacgaaa atctaaaatc agtgcttgaa tttttggaga
    42721 tttcaaatct ggcgcttgca aaagcgctgg atttggatcc ctcccttatc agcagatacc
    42781 tttcgggaga gagacgcttg tttgcggcaa gtgcgcagat gaatgcaata gccgatttca
    42841 tcattaaacg ctgcgatcag ttgcgggata tccaatggct tacggatcaa tttcattcgg
    42901 cgggactgct caccgatgtt tccgcaaccg gtcaattcaa acaaaatctt attttgtggt
    42961 tagcgtcgga tggtgagatt ctccggaaaa attttggcct cgccaaaccg gccgatatcg
    43021 gacatttcgc tccccgagaa aaaaatcgga gcatttcaac aatggatgaa gttcaaatcg
    43081 gcggtctgtc gatcgtatcg gaactttttc aaattcttca aaatcttcct caaaaatccg
    43141 tcatcgatat atttatttcc aatgatcgga tcggtacgat caccgatcaa gatatttcaa
    43201 acacattatt aaaacaaatc gaaaaaaatg atttgcatgt ccgtatgatc gtttgtgtat
    43261 ccggagatac tcaagcaatg tcccaaattc taaataccta tatcagcgcg ctggtttcgg
    43321 gacatatccg tttatccgtt atctttgcgg taacgcaaac cgtgatcaat caaatgactc
    43381 tcttgatccc cgatcggtat gttctgttaa ttaccgaaac aaacggcggc acggcaccgc
    43441 cggttgccgt tcccgtccga aattcgcaat ttatcaccga tatgggacga aactttgaaa
    43501 tggcatggca ttacgggcag ccgctgctca aactttatga cgataacttt tctcgcaata
    43561 ttctggaaat tctttttacc gagttttgtt ctcccggacc gcttgacgtg gtcaaggaca
    43621 gcatcaatcc catgtatatg ccggtaaaag cttatgaccg gttcctgaaa acgcgcggac
    43681 atcatcagga tgaattctct tggcggagcg cggaatttgc ccgctttaaa aagggaatgg
    43741 ataatgtgtt agctgcagac gtcccgtttc gagaaatctt gtcgatgtcc cgtctccatg
    43801 atatcgtctc gaaaggttcc tgccgtatgg ccggccttta ttttatggaa cgcggttata
    43861 tcgatctcga tacggaaggt tgtatagcga ttctcaacgg atatatcgaa tatctcaaaa
    43921 atgtgccaaa tttcaatctg gtgatcgttg atgatctgaa tcaacttcat cgcaacaatt
    43981 gttggcagct gaagcagaat caccaccttg caatcaacta ttggaacggt ccggaaccga
    44041 taatgatcta ttccaatcag attatgctcc tgcgggaatt ccagactcat tttgagatgc
    44101 tttgggaaca ggggcaaaaa tccttaccga accgggcaaa tgtaatcgct attttacaga
    44161 atattatcga ccatttgatt gaaaattttg gaacagagca aaaagacaat caggattcat
    44221 atccaatata aatttcatct tttcaacgga tgaaaatcga tataaacaaa aaggaacaaa
    44281 aaatgaaaaa gcatatgatg gttatagggt taatcagtct tttctatatt ttcataacct
    44341 gtggtgcgac catgcatcga tcttttaaaa ctcaggatgt ttcagtctcc gccggtgaga
    44401 atttcaaatt cggggatgaa acctatgccc tcgaaaaatg gataaaaatg ccgaaatatg
    44461 actcggaatc aaaaacaggg tacggcctca gctttttgca aaaagaaggg gtgatgccgg
    44521 tccaattatc catttcgacg aacggttcac caagcgctta tgcaagcctg cttgatatga
    44581 aattggaaat cgacggggaa aatcttgaac ccgaaaatat cagtttcgaa aatgttaaag
    44641 acgtacccgg ttatacagcc cgcattattt ttgaatttgc ggtaccgaat caatcaaaat
    44701 tacccgacaa agggacactt ttttatacaa ataaaaaaat acaaaaagaa attgatcttt
    44761 ccgcgttgga agcgccggag aaaaatattg aaatatccgc cgaaaatccg gaaatattga
    44821 atttttgcgg aaattggaaa ttatcatcga ttatttttca cactcccgat acagggaatg
    44881 gcatctttga aatcgacgca acattggaca tttcggacgg ttttgaatat gggcgcgatt
    44941 tgacactctc aaaggatatg tcgttaaaat cagatgtcga tatagacgcc ctgattgatg
    45001 cgatttccga gttgcctttt attgtgaatg atttcaatct gaagggtgat accggctggt
    45061 cgtattccga tggaaaaatg aagacaattc cggacggaac tgagttaatg gcgtcttaca
    45121 acgagaagga tgaagttctt cttttggtgc acagcggcaa agttgacatt ttatccaaaa
    45181 aaaccgacaa aaacgctgtt aaatccggcc cggtattaat ggagataaca gcggttttta
    45241 aaaaagcaaa ttagagaatg gggatgaaat ggaaagaaaa gtgctttgcc ctgtttgcgg
    45301 attaagcaac gacttcgttt ctccaaggag gatgcgatgc cggagttgcg gtaacaattt
    45361 tgaaattacc gaaaatgatt ttcagaatgt gaaattcatc gaaataaacg acggttatca
    45421 cagttggcaa tcccaaccca cagcttatca tacgatctat gcctgtttaa gttatcccga
    45481 atattgcgat ctctgtatcc ataagaatga ccttttttgc cgaaacggca aaattgactt
    45541 tcgggaaaaa aggatcaaat gaatcgccgg aaaaatatgt gatttgcaac agttcattat
    45601 caaacatgat catttcttat tcataccgcg atccgcatac aattcttcgg gtcacatatc
    45661 ggtgaatctt gaaaaaaatc agaacgaata gtgaatatga aggaaggaaa tcagagaaaa
    45721 tgcgtaaccc gattcgaaaa tggcaggaaa aacgacttgc aaaaaaacaa caaaaagaaa
    45781 aagaagccgc agaaaaaaag atcttattcg aacagcgatt ggaaagatgc aaaaaagggg
    45841 aacatgaatg gcagtattca tacgaagttc aaggggacgg agatccgaat gtcgaaggat
    45901 atgttttgat caccaaagcc acatgtcagg tttgccacga aaccgaagtg gaaatagagc
    45961 cttatgataa agaagacgat taaatagtcc agaagttttc gaaaaaaagc ggtcatggct
    46021 ttattcgtct gtgaccgctt tttttttact gaatcggata acttatccgt tcaccacatt
    46081 gataggatgt ccggcaagga attgttccaa attttcagaa gcaatgctca ttaaacgcat
    46141 tcgactttct ttcgaggtcc aagaaatatg aggtgtgatg atgcagttct tcgctttcaa
    46201 taacggattc tgatgtttga tcggttcttc gctgaccacg tccacagcgg caaaataaat
    46261 ttttccactg ttcaaagcat ccgccaaatc ctgttccgcg attaattgtc cgcggctgtt
    46321 attgactaaa ataacaccgt ctttcatttt gccgatcgtc tctttgttga tcatttcttt
    46381 tgtttccgga gtaagcggac aatggagcat gatcacatcc gaatccttta agagttcgtc
    46441 caatgagacc tgtcttgcga ttttctcccc ttccggactg agttcttttg tactggtaat
    46501 aacattcatt cccatcgctt tcgcgattct gccggtgcag gtcccgatcc gcccgagacc
    46561 gataataccc attgtctttc ccgatagctc aatcatgggg taatcccaaa aacagtagtc
    46621 cggacaggct tcccattttc cctcgtgaac aacccggtta tggtgaccga tttgatgaca
    46681 aacttccaat aaaagaccga tcgcaaattg agcgactgct tcggtgccat agctcggaac
    46741 atttgtcaca ggaatatttc gttctttagc ggcctgaata tccacgctgt tatatccggt
    46801 cgccaaaacg ccgatgtact tcaaattcgg agcggcatag attgactcgc gcgacacata
    46861 ggttttgttg gtaaaaacaa tgtcggcatc ccgaatacgg tctgacacct caccggtatt
    46921 ggtgcgatca taaaccgtca aatcaccgaa tttctccagt ttatcccaac cgatatcccc
    46981 cggattcagg gtatagccgt ctaaaacaac aagtttcata cattccatcc tgctttgaca
    47041 aaagataatt ccgaaaaata cagccgttta ttagaccagc aaaacttgca gaccaatttg
    47101 cgttaatttt tcgacgattt ccggatcgag gccggcatcc gtaataatcc cggaaaaact
    47161 atttaaagaa gccgatcgac agtttcccgt cgaattgaat ttggacgatt ccgccacaat
    47221 ataacttttg ttactgattt ttatcgcacc ggttttggtt aatgcgtcat ctatttcata
    47281 ggtagtagcg ctgtttcgaa caagatcgat tcccacaaca ccgataaagg acagatcaaa
    47341 ctggaacctt gacatccatt cgatggtcgc cgcaccggta aacccttctc gaagcttatt
    47401 catttttccg ccgataaaaa tcaactttac gcggctctca ccgggtattt cttccaaaac
    47461 attgatcatg ttggtcacaa tggtaacgtt tctgtttttc cgaaccaact ggcgggcaat
    47521 ttcgacattg atggaagaga gatccagaaa gacggtatcg ccgtttcgaa tcaatgtctc
    47581 ggctttttcc gcgatgcgtt gtttgacttc tatattattt tcatgggaat cgtctgccgg
    47641 ttggggaacc gaattttgaa ttaacggaat tgctcctccg taagttcttt tcagtaagcc
    47701 ttctttctca agttttgcaa gatcttttcg aatgagatcc ggcgttacct gatatctttc
    47761 gctcaaatcc tttaccaaaa cctttccgtt ttgatgaagg aattcaagga tctcccgttg
    47821 tcgttcctca acaaacatat tcacctcaag acggtcctat gaatttaatt gttattgaat
    47881 attgtatcat ttttggcgta aacgttgtct cccccaacaa tttctcccat tccaaagatc
    47941 gatttcgttt tttaatgggt aaaattaaat ccgtatgcag cccatcggga aaggagttcg
    48001 aaaatgatca gatatagccg accgcttgag gacggaagca actattttat accggaggat
    48061 cggattgttc gcattcccgc cggatacacc ggcgaaccga tcaaccgttt ggctgcttat
    48121 gaaaatttac aacaagatct attgaacgat cttgagaaaa tttcgcaaga aatggcagga
    48181 cttcgagaag aagggaagat aaaatcggtt catttcaaag aactgatgtc tcaaaaaatg
    48241 atcaacactc ggatcatctc actttttcaa agctatggat tggaatgaga tgaagagatg
    48301 aaaaaatccg ggaagaaaga atatcaccat tcaaataagg aaaactattc gcttgatatt
    48361 ttgagctttt ccgtttactt gcaataggat caggagaaaa aatggtcaat catacattta
    48421 ccgatgaaaa ttcgattcaa aggaaccatt acccggtaaa aattgggaac ctttttaaat
    48481 tatcgttcgg cgaagaggtc ggaaattcgg tcagtcacgg cgtttcggcc atgattctat
    48541 tattcgcgct ccctgcctgt gccatatatg gctatcaaaa aggcggaaag ttgcttgcat
    48601 ttggttacag tatctacatt atcagtcttc ttttgatgtt tcttgccagc tgcctttacc
    48661 atgcgatgga ttttaattcc aaacacaaac aagtttttcg gattttggat catagtgcga
    48721 tatatatcgc cattgccgga actttcacac cggtctgtct atcggttgtg ggaggatggc
    48781 tgggtatagt cgttttaatc tgccaatggg tgacggctat ctttggaatt ttattcaaat
    48841 cgctttcaaa caagaatctt ccgaaagctt ctctgacaat ttatctggtc atgggatggt
    48901 cggcaatctt gcttttgcct gcgatcattc gtaaacagct ttgggggttc ttagctttta
    48961 tacttctggg tggtttgctg tacacagtcg gaacgatttt ctattccaag aaagaaaaag
    49021 gttggttcca tttcatctgg catttgtttg ttatcggcgc atcagccgct cattttgtcg
    49081 ctattatttt ttatatctga aaacaaacag gatttgacac tttatcattg agagaaggga
    49141 aaatcgtgga aactttttgg ccgattattt tgattgttat ctcaaacatt ttttacaaca
    49201 tcaccaccaa atccactccg gcggatgcca atcctttttt atctctgacc gtcacatatc
    49261 ttgtgggagc gatacttact tttcttatgt tttgggcaac cgccacagag aaagggctct
    49321 cttccaatat cagccgatta aattggaccg gttattgtct gggaatcatc atcgttggcc
    49381 tggaaaccgg atatatttat ctttatcggg ctggatggaa catcagcctc ggctctttgg
    49441 tagcaaatat cctcttggca atttctctcg tgatcatcgg agcccttttt tatcatgagg
    49501 agatttctgc ccggcaactg atcggcatgc ttttttgcat catcggtttg atcttcgtca
    49561 ataaaaaatg atttatgaac aaaaattgaa ttcttattga gaattcaaaa ggatcgatga
    49621 atcctattga ttgtctaaaa agaggaatga agctgtctgt ttcattcctc ttcggtatga
    49681 ttaatcgact ttttttgaag gatcaaacaa tttcaataaa tatccgtagc cttccgcttc
    49741 catctttgat accggaataa atcgaacagc ggcactattg atgcaatatc gctgtccccc
    49801 tgattctgcc ggtccgtcat caaaaacatg tcccagatga gaatcgccgc tgcggctgcg
    49861 gacttcggtt cggaccatcc cataggatgt atcggtgtga tagttgacca cttcatcgga
    49921 aataggtttt gagaagctgg gccagccgca atcggaatca aatttatcca gcgaagaaaa
    49981 aagcggttct ccggtgacga tatccacata gattccgtca tcaaaagtat tccaatatgc
    50041 gttggtaaaa ggcttttcag tcccgctgta ttgtgtgaca ttgtattgtt cttccgtcag
    50101 catcccttta atcgcggaat ccgatggctt cggataagcg gtttcatcaa tcaacggctc
    50161 atcggcaagc gaaagatcaa tatgacagta tccgttcgga ttctttttca gataatcttg
    50221 atgatagtct tcggctaaat caaaacgaat caagggagtt acttcgacct gaatcttttt
    50281 atcatatttt ttctgttcca tggcgatccg agtttcaatc accgctttat cggattcatc
    50341 gacataataa atgccggtac gatattgcac tccgcgatca ttcccttgtc tgttgatact
    50401 ggtgggatcg ataattctga agtaatactg cagcaactga tccaatgtca cccggcttgg
    50461 atcgtagatt acatgcaccg tttcagcatg accggagtgg ttatacacca aatcctcata
    50521 actggggttc tcggttttcc cgttcgcata acctaccgtc acttctttta caccgtcaat
    50581 cttttcaaaa taagcttcca ctccccaaaa acatccgcct gcaagataaa tttccgcttc
    50641 ggcggaaggt tgagaatttg ctgagccacg aatggcatcg ttttctgaat tgggttccat
    50701 ttcttcctgc tctttctgca tattttcttg aacgggatca ctttctccat ccaagagatt
    50761 tgatttaaaa atagctttca ccatttcatt tttgatttca cccgggaata ccttaatcgt
    50821 cccgttggcg ctcacaaaaa aagaagtcgg atagccgtgc acaccatatt tcgttgcgat
    50881 ttttccgcct tcatccaata aaacccgaaa atggttattc tccaaacccg caaaccattc
    50941 ccgaaattca ttcgtgtcca tttctttgcc gagtcccggt gacacaattg tcaccacctc
    51001 aaaatcattc gattcggaag ccagtttttc tagttccggc agtccttcta aacatatcgg
    51061 acaccaactt gcccaaaatt taatatagac cttcttccca agctgctcgg ataatttaaa
    51121 ccgttgtccg tccaggtcaa ccaaatcaat ttcattgggg ttttcgccgg attttgaact
    51181 accactacaa ccggaaatga ttaaaatcaa gatcaacatc caaaaaagaa atgcaaattt
    51241 cttgttcttc atttacaacc tttcatcaac cgtttaatcg attaaaaaac agtaacagtc
    51301 cggtgatcac caacaaagcc ccgccgattt tccggacgac cggcaaccat tgtcccccct
    51361 ttttaattcg tatcaacaaa aaatcagcaa acagcatgat gaccacaaag gggattgcca
    51421 ttccgagtgt atagaaagtc atgtaccaag ctcctcgaat cgcagttccg ctgttcgcag
    51481 ccagaatcaa gaccgaagct aaaatcggac caatacaagg agtccatccc aagctgacaa
    51541 ttaatccgag aaaaaaagcg cttaaatatt ggttctttcc cgatgagtta taacccggtg
    51601 cgttccattt ttccataaaa ttcaagcgaa gcacacccat ttgattgagc ccgaacaaaa
    51661 ttatgattaa tccgccgatt cgatgaaata agggattgct aaacaacgga gcaagactgc
    51721 cggcaccgaa tccgagtaaa ataaagacaa acccgattcc caaaataaac agaatggttt
    51781 tcaacaagtt ccttccggta tctttcttcg cttcgattga attttcggga gtggaagtat
    51841 ttgtcaggat tccgaaataa acaggcagta aaggaagaat acagggactg aaaaaagaca
    51901 agatcccggc tgtaaagaca ctacctatca tcagattctg gttcataatc aaacctttct
    51961 aaaattttat tttaaagcat cggacggaag gaaaggaaat attaatatct ttttatcatt
    52021 taaagatgat caattcaaaa a
//
LOCUS       DAOSMM010000007        50944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_717_length_50944_cov_19.241192, whole genome shotgun sequence.
ACCESSION   DAOSMM010000007 DAOSMM010000000
VERSION     DAOSMM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 50944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50944
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_717_length_50944_cov_19.241192"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..2913
                     /locus_tag="PK898_02245"
     CDS             184..2913
                     /locus_tag="PK898_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOU18885.1"
                     /translation="MRNIFTILLRFLGLWVFNAICLLACEWVIPGITIIPYQGVPYAV
                     TVMEVSLIFSIMNLFVRPLILLFTLPLNGLTFGLFSLVVNALVLFLASEFTPFFKIEY
                     PIAAFLSGFLFALVNIIITSLIPIDEDFLYYDILVNRFRKSGEKINKDKKGIIVIEID
                     GLAYPRLIKAIEKRKMPYLKELIDSGKFTISEFDCGIPSQTSSCQAGIMYGDNQNICA
                     YRWYDKEQKRIISSSSFQDTNQMEQEILRRKKNGLLDGGMSINNLMSGNADVSLFTVS
                     AIQPKNSEELNNRNINLYFFSLRPYLLTKSILFALADAAVEVIQYVWACLKRKEPRLN
                     RLVHFYPLIRGSTNVLLRDASTALTINEAMRGGPASYTTFYGYDEIAHHSGPDSFEAY
                     HALSGIDHSIKKIHHTAEENSDRDYELFVISDHGQSFGKTFRQRYGYSISDYIRKLAG
                     ECARLKSESQVIGIGNSAADNESSIRAAIYDLTAQKKTQQIPIPKQTLNKLDTVLEEN
                     ADETRAKKRPEDNDIWILASGNLANVYFSFSNQKVDYAEIESHYPNLCYRLIQHPGVG
                     LMVVHQNKTAIAIGKKGSRNLSTGEISGQDPLANYQPTEMRSRQLSYLAEFPNGGDLI
                     LFSTFYPDGTVAAFEELIGSHGGIGGEQNTAFMFHPINVEVPENIFNSKEVYAILKGR
                     RELDAEIDKFSEEKTKQNNGSEDWKWDKLIAGIRDTSKWTKYMREVILFQSSIYKKIS
                     KDTSLNGPAVLIMMMSLLSNMTVAGILFRSLKGLYLGLIIWLVSWSIVIGASYGATLA
                     LRQNIIFSDFLRGMIFCTVFDVLLFGALIPGYENFWIVIILVTRLISVSLAINGICNI
                     QGKRSLLIVPVVLFMLILLVITTYVILESLGYILKIDPLIDFINHINEIVGIS"
     gene            2985..4751
                     /locus_tag="PK898_02250"
     CDS             2985..4751
                     /locus_tag="PK898_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HOU18886.1"
                     /translation="MGLSRKIIKNQSEILANHPELVQKRIAICGTCNIRFISSGLIPF
                     LMAEGIFPLIFEGKFNGLSSEILSSDSDLYAFDPDYLIAITDYRDIHEFPPYLTSIEK
                     VRETANRRIEESIHFWEYFHQRCRSKVLLTNIAIPNRHILGGLEANLVSGRDVFLRIL
                     NLTFLEKKPTFLTFLDQEALSADFGKDRWFDDSEWFISKQPFALEAIPIFSLTIAKQI
                     AADIGKIRKCLITDLDNTLWGGILGDDGINGINLDPNDPIGESFLDFQKTLKLYKDRG
                     ILLAVCSKNDPVIAKEVFQKHPNCILKTEDFSAFIADWEDKPINIQKIASQLNIGLDS
                     LVFFDDNPAERDLVKKVLPMVQVIDVPEDPAEFSMALIRSHCFDWGQLTVEDLNRSET
                     LRADRKRSELGAIALNYEDYLKALEMRISIAKALPHELNRFTQLINKTNQFNLRTRRY
                     NESEITAMATDPDKVLLRVTLQDKFSDYGIISAIILNLDHSRKNILIDTWVMSCRVFK
                     RGIEDEVMNYLIAFAKDNGFQTVEGEYVCSPRNGLVADLYPRYGFKMVQQSDISKKYI
                     LDINDYVPCETWFNFELKGKES"
     gene            4748..4996
                     /locus_tag="PK898_02255"
     CDS             4748..4996
                     /locus_tag="PK898_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU18887.1"
                     /translation="MTDLEKIQSIMRDIFDNETLEIDESTSAEDIAEWDSFSNIQLIA
                     AAEQHFAVKFTTQEAIHIRTVGDILNLIRQKKLPVDKL"
     gene            complement(5079..6215)
                     /locus_tag="PK898_02260"
     CDS             complement(5079..6215)
                     /locus_tag="PK898_02260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU18888.1"
                     /translation="MIKVLHVITDLNTGGAEKMLAQVIPVLKSGGIEQSVVCLRSTGP
                     VAKIIEENGICVTCLNMKPAVPSYSAFRRLKKTAADDRPDLIHSWLYHADLYASLVGR
                     KLQIPVIWGLHNATLSPNVKLSTRLVVSQLAHLSQSVPRRIISCSCSGKQNHIDMGYR
                     GDIITVIPNGFDTDRFKPNSEMRRKKRCELGISDDNILIGNISRFDSQKDHLTLIQAW
                     KELLQKVPGIHFRFLLVGSGLNWENKELRDWICDAGLESRMLLLGERDDIPALLNAID
                     IFTLSSIGEAFPLALGEAMSSGLLCVTTDVGDAPYLLDDCGILVPKNNPAALAEAIRK
                     MVFLSAEEQDVLRFKSRQRIIEHFSILQMGMAYLKVYQECLSIG"
     gene            complement(6381..6866)
                     /locus_tag="PK898_02265"
     CDS             complement(6381..6866)
                     /locus_tag="PK898_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU18889.1"
                     /translation="MGKTNRGKIVLSKIFTLSNKIQILGDRLETGITIKQFQLLEVLS
                     KNDPAPMTITELSEALGSSRQNIKKMALLLEKQNYLVLSKDDTDGRIVRVALTEEGKN
                     SLQQQAESDKEFINQLFDGFDDKLLKNMSKGFDKLSKNILLMEEKTNNPAVPKETIKP
                     F"
     gene            7213..7908
                     /locus_tag="PK898_02270"
     CDS             7213..7908
                     /locus_tag="PK898_02270"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOU18890.1"
                     /translation="MRDQEKILLASGSQRRRELLSWLTDRYEIIPSDIDESPLPGELP
                     QVYCQRIAEQKALAIPSRTETQNSFVLAADTIVYSQNRIIGKPKDEQDAFQILTFLQG
                     QIHHVCTAVTLYRNGKTVSTLCDTLVRMNPLSQATIRSYIATGDPMGKAGAYAIQNTD
                     FNLVESVEGCYTCVIGLPLCHVEKLFSFFDYSFEKNVIECCKSHIHYNCVVDQSEMLR
                     KSLFNQKIIERNV"
     gene            7905..10166
                     /locus_tag="PK898_02275"
     CDS             7905..10166
                     /locus_tag="PK898_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOU18891.1"
                     /translation="MKRFIICFIFLCVFTAGCGPKVSNESTKTPEITTVMPSDTPLPE
                     PVVMITSAPEARTTANTFLEFWKADDFVSMYNMLSQVSRDAIPFEDFETKYKEAETNL
                     TLQSLNCEVTSALTNPKTAQIGYHATYQTALAGTFSRDMVMNLVFEANDWKIQWDDGM
                     IMPELSGGNKIEIDIDVPTRGPIYDIDGVPLAAETEAYAIGVVPASVPSNRWNGLINE
                     LSRLTGKTTFVITQLMEEANQYDYVVIGEVPAAVVEERMEAISSYEGVYLNSYTASRF
                     YYEGGSAPHLVGYVQPIGEDEAETMKRQGYRIDERIGRIGIEKWGQDRLDGERGASVY
                     VVDSAGNPITRLYKSDPVPAETIYTTFKEDFQYDLQRAIAGFRGAIVVLERDTGRVLG
                     MASSPTFDPNLLDFNNYNSFYSANLLYNDDRPMYNRATQGQYPLGSVFKIITMSAALE
                     SGVYHVNDTYDCGYEFTELPGITLTDWTLDHDVAASGKLTLSEGLMRSCNPWFYKIGL
                     ELFRQKGADYLPSMARGFGLGSPTGIGEVEEESGNVETPPDEYSSVQMGIGQGTLLVT
                     PLQVARFVAAVGNGGTLYRPQVVEQIVDYEGNVTYAFKPEEQGKLPVSKENLELVQQA
                     MLSVTNNPRGTAYSTFKYIKYPVYGKTGTAQTSPGEDPHAWFAGYTDRKLEGKPDIAV
                     AVLVENMGDGSVYAAPIFRRVLELYFDGKASMLYNWEAGVYITKTPTPTPTITPTPEP
                     TKAPWQITPEVTP"
     gene            10179..11087
                     /gene="rsgA"
                     /locus_tag="PK898_02280"
     CDS             10179..11087
                     /gene="rsgA"
                     /locus_tag="PK898_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOU18892.1"
                     /translation="MTLQGRVIRYQSGFYTVMTDERIVTCKIRGKLKENIRQTDLVAV
                     GDLVEFTQFQDGTGVIECILPRKNELIRMISGIKSEYRQVLISNLDQILLVFAVTQPE
                     PRLRMLDRFLVICEKQGIPPLIVANKVDLSGMDQAKSVFSVYSDIGYDVLYTSTASDI
                     GIADLKERLQGKVTGLVGPSGVGKSSLINRIDPRLNLKVNEISNYNRKGKHTTVVREM
                     FPLKEGGFVADLPGLKTLALWDIQPEELDGYFPEISPLVSKCLYGDCTHSPSEVGCAV
                     HEAVKEGKISRERYESYLRIRFGEPE"
     gene            11191..12228
                     /locus_tag="PK898_02285"
     CDS             11191..12228
                     /locus_tag="PK898_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOU18893.1"
                     /translation="MPKRLTWTELILNLKSPFTVSYGSSDTRTAYWIRLEEDSGWGEG
                     TIPFYYGIDFKDMTDFWDRCALREDPFPETINAIHPWIGDDAPAPAIAALDIAFHDRI
                     GKKTGKAIYELYDLPAPHPMTTSYTISIDTPENMAQMALEINRYPLIKIKLGSPGQED
                     LDEARIAAIRTVRPDAKLRLDANAGWKTDDAVKLVKRLEKYNIELIEQPTAKFDILGM
                     GHVQTETPIPIVADESLQTINDIEKLHDVGVRGINIKLMKCGGIAPAVEMIRKARSYG
                     MKIMLGCMVETSIGVGAMASLIGLADWIDLDTPLLISNDPFSGLTYTEDATLIPPAGP
                     GLGLKLKNSER"
     gene            12237..13253
                     /locus_tag="PK898_02290"
     CDS             12237..13253
                     /locus_tag="PK898_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOU18894.1"
                     /translation="MTFQKIETILADVKKNFFNEPMLTVFDIQPEIISSNELKLTGKI
                     LEQKDLDFLKNELENKIPDWKVDYKGIKVLLNEKTTILRVSKNLTDMHREKSFLSELT
                     NQMLYGDPVAVLADEGEWVLGRNMSDGYISYTYKKYLGEIPWSEPTHIVSEPVALAYN
                     NPNREELVTRVLGGTETVIFDEKGDMAFIRADHNGWVKKTSLIPISDLPTDQDKIRNR
                     VCENAKSLIGVPYLWGGTSACGIDCSGLVQWSYRLCNIRLRRDAHMQLIPEKCVEPPF
                     LPGDVVLYGEEENGKKSITHASISLGGWTVIHSSRSQNGVYIDDALIAPALKGTFAAA
                     VRYI"
     gene            complement(13322..14074)
                     /locus_tag="PK898_02295"
     CDS             complement(13322..14074)
                     /locus_tag="PK898_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HOU18895.1"
                     /translation="MDRERIEKELSTLDGEYTFYYSDQENHPIAVSNRDESHRFLTAS
                     VIKVPLLFAWYVSEKEGFVSSDEICDPSSEPIVKGAGFYYLFFQKALPYHDLLLMMIA
                     TSDNYCTNLIINKLGFERINHIFSETFHFRDTHLGRKMMSKPDLARGYDNWTTTADMI
                     HCYDLFHSFPAEAKKNISEMLSVCQADKLFTRNLKGDSVDFYHKSGGLHNVINEWGFT
                     DRCRIFLLCNNYSDYQKVYDVFGNLGKWLLDS"
     gene            14145..14489
                     /locus_tag="PK898_02300"
     CDS             14145..14489
                     /locus_tag="PK898_02300"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HOU18896.1"
                     /translation="MVYHICTQTSYESVKIRGKYSPDSLGKEGFIHFSSIRQILETAN
                     SFYRGQDDLVLLEVDEQLTDQIRFERPADHREGLFPHFYGELPVELIRRVLPFPHQAD
                     GTFKLPEEIAES"
     gene            14490..15809
                     /locus_tag="PK898_02305"
     CDS             14490..15809
                     /locus_tag="PK898_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOU18897.1"
                     /translation="MLEESQFKLIDELIRAALTAVDPENVVKNALDLKNSILKIQNQP
                     FDLSEFEHIYLIAFGKASLKMAKATDDLIGRFITGGIIVSKKKNSEIRLPDKYTCLVG
                     GHPIPDQNSLESAQRILNLVKDAGNRDLIICLISGGGSALLTLPENGISLKDIADLTK
                     LLLENGANIQEINAIRKHIDRIKGGKLAIAAAPARIVTLILSDVIGNSLEAVASGPTV
                     PDSTTYRDCLEIIQKYHLSEKIPAAIYERILIGAGGDIPETLKDDSPILKNISNYLIG
                     DNSTAISAIVEKGKSLGLNVSVIPKPIIGEAREAGRFLAAELRKPVTFKPSLLVGGGE
                     TTVTIKGKGLGGRNLETALAAVKDLAGIPNIALVTLATDGEDGPTDAAGAIVTGETFS
                     EGMKKGLDPDLFLDNNDSYHYFEQMGGLIKTGSTGTNVNDLVFLFHF"
     gene            15978..17750
                     /locus_tag="PK898_02310"
     CDS             15978..17750
                     /locus_tag="PK898_02310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOU18898.1"
                     /translation="MNQGKKYYQKSWSLEDLYSGTNSEEYQAAMNELGHLTDEIQNYR
                     EMLSPEISVSDFGTILQKLEKFHEIASRLLSYAELLFSADTQNSEAMSLVGQTEAIIT
                     EAHNKIIFFSLWWKSISDDEAQRLIKEFPDYQYYLKQLRNTKQFTLPEFAEQIINIKD
                     ISGKNALNTIYESLTNRYQFTLKIDGKSKKVSRSGLMQYVRNENPEIRKQAYRELYRV
                     FGGDGNILGQIYQSIARDWYFENVKIRKYPSPISVRNIENDIPDQVVDLLLDTCIKNK
                     SVFQRYFALKKEMLALETFTRYDIYAPLQKEERLIPFEEGLSIVQSAFTDFDPEFAAM
                     AMRVLDEDHIDSADRRGKRDGAFCLTTSPKLTPWVLVNYQGRISDVSTLAHELGHAVH
                     SLFANRQNIFQHQACLPLAETASTFAEMLLVDALLKEEKNPDIRKELIMQQMDDFYAT
                     IQRQAYFALFEKKAHEMIASGTTVDQLNAVYLDNLHDQFGEVVSVSDEFKWEWVSIPH
                     IFATPFYVYAYTFGQLLVLSLYKKYKEEGKNFIPQYKQILSAGGSKSPEDILADAGFN
                     FYDPAFWQGGFDFLTEKMMEIERN"
     gene            17812..18804
                     /locus_tag="PK898_02315"
     CDS             17812..18804
                     /locus_tag="PK898_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18899.1"
                     /translation="MTQTFLSKRLHSDHSIVCVYLTGSMLRDDPFINGTADVDLVIVH
                     NQPVDEIREIIGMTPEVTLDIHHIEQSYYSPPRKVRKDPWIGSSLCFDPLLLYNKGHW
                     FEFMQASVEAGFFSPEYVIHRSGLFSNEARLLFTELENQRNLGSSIYISSYLKIIEDG
                     CNAVACLSGLPLTDRTLMKRFNEVAEAINREDLAPILYGLILGQSSPFPYYDYFFNSW
                     KYYLEYFGNSDLAPLKMKADRIPYYTAPVSYYWNEHLASALWIMAKTWCSVAKAYQLD
                     DNEYFGTFCNMLEISPMFFAKRKTQLELFLDSIEDAQKNWGDSQGLDANALENL"
     gene            complement(19074..19247)
                     /locus_tag="PK898_02320"
     CDS             complement(19074..19247)
                     /locus_tag="PK898_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU18900.1"
                     /translation="MAYKIDPDVCIDCGACESECPANAISEGDGYMVIDPKLCEECGN
                     CAEVCPVGAPSKQ"
     gene            19850..21751
                     /locus_tag="PK898_02325"
     CDS             19850..21751
                     /locus_tag="PK898_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU18901.1"
                     /translation="MPLISAQKLSKSFGAEDIFSEITLSIPRGAKIGLVGPNGSGKST
                     LLQILMGITPSDSGTVQRAKKITIGYLPQNFEAEFEKSPYQECLSVFSDLIMMKSKLN
                     QMEQDLANGNNVSELLSSYGNLQNTFESRGGYVFETRIHQVLQGLGFSKSEENRPWYQ
                     LSGGQKTRAYLAKILLMNPDLLILDEPTNHLDINAIEWLESFLKEYKGGALIVSHDRY
                     FLNQTVEVIWELDSSFEIYHGNYDAYLQQRQARYERQKAEFESQQEFIAKEEEYIRRN
                     IEGQNTRQAQGRRTRLERLLEESKITNPILHRKIQTNLSSSIRSGDIVLRTKNLEIGY
                     LDDHKKLISIPDVVLQRGECVAILGPNGVGKSTFIKTILNEIPPLAGEVEMGSNLKVG
                     YFAQAHEKLNPKATLMDEITAAAPQMLPAEIRSYLARFLFTGDDVFKTTETLSGGERG
                     RLALAILALQNANLLLLDEPMNHLDLEAQETLQALLKDFNGTILLVSHDRYLVDAIAT
                     QIWEVNPDKKTLEIFRGNYSEYKEYQKKSGISELKDQIDSTERKSPEIRENPPLQKLS
                     NNEIFRRQKKKEELEKRISVLEKMLNEISQQMVSQKEDHQKIAELGIRYNSIEIELNS
                     VMEEWAEID"
     gene            21779..22732
                     /locus_tag="PK898_02330"
     CDS             21779..22732
                     /locus_tag="PK898_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU18902.1"
                     /translation="MYLSVIAPVYNEHDNIPLLYEEMVKALKDFDKEWEFILVDDGSV
                     DGSFEELTKVAEKDPAHVKVIGLRRNFGQTAAIAAGVDYSTGEIIILIDADLQNDPAD
                     IPMMLGKIDEGYDLVSGWRKSRQDNLSRKIPSVIANKIIGHVTGVPLHDYGCTLKAYR
                     REVITGFRLYGEMHRFIPAYANTVGARIIEVEVKHHPRRFGKPKYGLERTAKVILDLF
                     TVKFLSSYSDKPIYLFGGSGLFLTIIGGLLLLFLAVRRLGWGVSVLASPLFTFSIMIF
                     ILGFQLIMMGLLAEMLTRSYYESQGKRTYTVKKLINFKSDK"
     gene            22729..23862
                     /locus_tag="PK898_02335"
     CDS             22729..23862
                     /locus_tag="PK898_02335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU18903.1"
                     /translation="MKILTVIHEFPPIGGGGGLIARDLACALVQDGNEVRVITAQFGD
                     LPDREVLDGVEIIRLKSHRKEAYRAKLLPMASFILAASRYCVYQMKDFSPDIIHAHFA
                     VPGGPPAKFISGKKHIPYVLTVHLGDIPDASPEKTGKWFKFIYPFTHTFWKHASAIVA
                     VSEFSKRMAQRKYPVEIQVIPNGIDYDQVRIDRIEPHSVPEIVFAGRFVPQKNLEQLV
                     KVCAAIKDLNWHCTLIGDGQDREKIEELIERLNLQNKISITGWVAPERVTEIFKQSDI
                     LFLPSRSEGLPVSGIQAMTCGLALVLSNAGGNPEIIEEGKNGFSFDPEDTDSFASALR
                     ALLESPEMLLSFRQNSLESAKKYDIKRIAKQYEAVFEQVLAAK"
     gene            complement(23866..26520)
                     /locus_tag="PK898_02340"
     CDS             complement(23866..26520)
                     /locus_tag="PK898_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18904.1"
                     /translation="MGIDPSQEKRGLKPIPLFFSLLTVVSLICGYTLFKIPSDQKNAF
                     ILGLSLERVVMLAFFLLIALASLIGAIIFNINQQKNASLEASILACKEQIKRIASVTA
                     VLSGGLLLMPDYRFGQFSAYFHRLRPFLFLLFWVGFSAALILIVFSDSNIFKRIKDDC
                     RQITGKPFFIFLGLFLLTILFIYGSGWGIVAGKEAWYGNAVPVESLQIVLIIGFLLLL
                     SPFMTKIGRVKLFRNRAFLFFLIWGIAAFTWSMAPMQAHFFAPGPYPPNYEYYPYSDA
                     LLNDVAAQTAVYGLKFYHGTLVLKPFVTFVIYLCTLLSGNQMNLTLLIQSAIFAVLPA
                     ILFLFASDLGGLFSGFLAAALMIFQEWNALHTTQILTIHSRLEMSEFLAEVLFAAFAY
                     FIFKWFRQGRKQTYFAAAAGGVLGLLIYTRFNFVAMIPAIILFGGIVFHKKIKRGIRD
                     LLIFLLSFVICISPWVVRSYHITGTIAPEILDSFKSVVVEQRLKPITETSEISVENKE
                     PVQATAEPVLQQHTEATPATGKQIEKETETQLKSFSLIPDEIRLKVHPLIDTIGNHFF
                     HNLFSIAFILPVQIQFDDLNHLYTAEDSVWRDHWNGSLSAGQILFLIINLILISIGLN
                     LLWKKESWAGLSAAYLLIVYAAGLGLARTSGGRYIVPMNWGVLLIYSIGLAVILKKIT
                     PIRTDLVTGSTEISNQTIGIGDFGKLVLITGIFFMAYFSMILIEKNTSPSIPSENELI
                     VTEKMKETFPDQDWTKAETQLREGKMRLFQGVALYPRFYYFNQGEHGTDKAYAYQLYS
                     RIIFKILFDNGESDIVLPLDKFPDTFPDQSSVAVIGCLEPGSSYIKGIAVIGQTPDGK
                     EFHYLRSPMKEIACPIPEPVCPEVNKCY"
     gene            complement(26484..27569)
                     /locus_tag="PK898_02345"
     CDS             complement(26484..27569)
                     /locus_tag="PK898_02345"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOU18905.1"
                     /translation="MILPEISTGKIFETVFVSFFLCLIIVPIAIRFSHKTNLIDFPLS
                     EPHKIHKRPVPISGGLAFFATLILTAVFLNHRLDSQTGKVLLCATIIFGFALWDDYRP
                     LRWRWKMLGQILAAIVLYFCGIQVQIMQTVGEILGFSATVNDLLDFGITIFWMVFITN
                     AFNLVDSMDGLMVGLSLLALIFFVLAAMDTKQWSLALLCSAMLGACIVLNFFNSSPAI
                     LFFGDSGAQTIGFLIAAIAIIYNPPDRLQTSTWFMPILLLGVPIFDTVLVIISRFRRH
                     LHFYDSGTDHTYHRLVKMGFSDVQAVNLMLLAATALEILAFFIISRPPLTANLIFFAV
                     VLAGIFLIYFFESENMWESIRLKKNGD"
     gene            complement(27566..28684)
                     /locus_tag="PK898_02350"
     CDS             complement(27566..28684)
                     /locus_tag="PK898_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU18906.1"
                     /translation="MKVIFFANTDWYLYNFRLDFAKYLREKGWEVVLMAPAGEHTEKL
                     TAAGFRFIPFEFSRKGMNPFIEQRTIQRIKKIYAEEKPDLVHHFTIKCVIYGSLAAKE
                     VGIRAIVNSITGLGYIFLDNKITTKMIRKIVSSLYKKALKDTEVIFENPDDAALFEKY
                     GFTTEGNTHIILGTGIDTELFRPIPPPDSIPLIILPARMLWDKGVGEFVKAAEILKSK
                     SLQARFALVGKKDDGNPSSISYEQLSFWQKEGNVEWWGWQEDILTVISISDIVCLPSY
                     REGLPRILIEAASCGRPIVTTNVPGCREVVVDGVTGFLVPAKDPKSLAYALEKLIRSN
                     EMRIEMGTAARKRAIEFFSISRVNDEIFKIYQQLTQDS"
     gene            complement(28750..29409)
                     /locus_tag="PK898_02355"
     CDS             complement(28750..29409)
                     /locus_tag="PK898_02355"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU18907.1"
                     /translation="MDDSYEKMTQLAQQIAVCEKCQLHLSRKKSVPGEGNIHSKILFI
                     GEGPGLNENETGRPFVGQAGNFLNELLSTAKLKRQDVFITNVVKCRPPANRDPLPEEL
                     TACSSFLDEQIRLIDPLVIVTLGRFSMGRYFPLARISSIHGKGHWVDGRMIVPMFHPA
                     AALHQPNLRDSIMHDFALLPTYIHEVEEKITAEPVENANPASEAAEKKEATDGVQLSL
                     F"
     gene            complement(29385..29903)
                     /gene="rimI"
                     /locus_tag="PK898_02360"
     CDS             complement(29385..29903)
                     /gene="rimI"
                     /locus_tag="PK898_02360"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOU18908.1"
                     /translation="MIGRTDISIRRMVLTDIPAVFTIDQASAALYWPQSSYYFEVERN
                     EASRPWVAVDSQQRVCGFLVLWLIMDELHVANFAVHPDYRRRGIGDALMQNGLYEGWK
                     EGARISYLEVRAGNSAAISLYEKIGYRVVNIRRHYYQDNQEDALMMNLEMQDYQTYIL
                     TLEKLWMIPTKK"
     gene            complement(29906..30574)
                     /gene="tsaB"
                     /locus_tag="PK898_02365"
     CDS             complement(29906..30574)
                     /gene="tsaB"
                     /locus_tag="PK898_02365"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOU18909.1"
                     /translation="MLLAIDTSTSNIGIAVYDGNSIIGEANWLSLNRHTVILAASVKN
                     LFDTVDTDISKLRGIAVAMGPGSFTSLRVGLSFAKGMALGLDIPVIGVPTLDILAAAQ
                     PINGLPLCAVLQAGRTKLAAAFYNPSKDKWAESLPVSVYTTESLCEAITEPTTVCGEL
                     TADMRTYFRRNNKNIRMASPAMCLRRAGFLAEIAWARLEKGDYPPPNTLSPIYLHTKE
                     PISQ"
     gene            complement(30581..31075)
                     /gene="tsaE"
                     /locus_tag="PK898_02370"
     CDS             complement(30581..31075)
                     /gene="tsaE"
                     /locus_tag="PK898_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU18910.1"
                     /translation="MPILDSETLEIFSRSADQTRRFGMRLGRMLQNGDVLCFSGDLGS
                     GKTTFIQGIAQGWGSVDQVTSPTFVLVNEYYRMDGSTLFHMDAYRIDSTLEAEELDID
                     RMLHQGVLVVEWAERIDSVLPKERLTIKMAWVADEQRSLVMVPYGERYQTLLHSFRHR
                     SFGV"
     gene            complement(31136..31360)
                     /locus_tag="PK898_02375"
     CDS             complement(31136..31360)
                     /locus_tag="PK898_02375"
                     /inference="COORDINATES: protein motif:HMM:NF017879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOU18911.1"
                     /translation="MDEEYELGDLITLKKMHPCGSNQWVVVRLGAEIGLECLNCHHRV
                     LLTRRELSHKMKGKPQKNPERPSESSEPST"
     gene            31439..32062
                     /locus_tag="PK898_02380"
     CDS             31439..32062
                     /locus_tag="PK898_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOU18912.1"
                     /translation="MAVPAVFVCRILTLIIAFTIHEFAHGFLADRFGDPTPRLQGRLT
                     LNPLKHLDIFGSLMLLLTGFGWAKPVQIDTEAIRRNNKAGVMLVSFIGPFSNFLLALF
                     GALLLKIPGITTIRWWGIPFFLSQFVWINLSLLIFNLVPLAPLDGEKVVEYFIPDSML
                     PAWKNVQQYGMQILLICFLILPYLNIDVFGKILSPLISGLYYWLVGG"
     gene            32067..33053
                     /locus_tag="PK898_02385"
     CDS             32067..33053
                     /locus_tag="PK898_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOU18913.1"
                     /translation="MIQIAIIGLGEIGTSLGLALKYQKDEIYRVGMDVHKYATDNASE
                     KGAVDRVSRNLLDAVKEADIVFLAIPAHEVEDTLELIGHELKKDAAVLDTCPTKSAAI
                     NWAKKSMSQPDHFLGIYPSINPAYLSEPSNEFHSAHEDLFQSGTLYLAPSADTEPAVV
                     KLASDLGTLIGAHSAFIDPAELDGLLAAAYDLPLLLSNALMSTLLNQNSWEDCRKLAV
                     KEFVQETDFCAFPYTGKEFTEILSENRENTLRILNDFIVNLKTYRDAIQNRDRAALSS
                     LYEKAEKGREKWLKDYEEGAWRKEKDQKLNVPTAGEMMGQMFLGGLGRKKKE"
     gene            33119..33397
                     /locus_tag="PK898_02390"
     CDS             33119..33397
                     /locus_tag="PK898_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOU18914.1"
                     /translation="MVECSNGSFYTGWTTNPERRLKAHNSGHGASYTKMHSPVSLVYV
                     EKMESRHEAMIRETEIKKYSHAEKAAMSESWKKSTVSEERDRKDQQGK"
     gene            33398..34312
                     /gene="era"
                     /locus_tag="PK898_02395"
     CDS             33398..34312
                     /gene="era"
                     /locus_tag="PK898_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOU18915.1"
                     /translation="MENIQTFRSGFVSVIGKPNVGKSTLINYLLGQKIAAVSPRPQTT
                     QRNQLGIITEKDYQIIFIDTPGIHQPKSKLGDFMNEAAVGTFTEVDIILWIVNLNEPP
                     AAEDQIVAEKIKNAGILSNTVLALNKADLVSPANSERYADRYRALVPGAESFLISAKT
                     GAGCDDLLAALVRRLPPGPAFYDEEQITDLYEREIARDLIRESLLKNLEDEIPHSIAV
                     RIDEYADRTETKSYIQATLILDRENHKSIVIGKNGEMIKKIGQDARSEIEKLTGRSIY
                     LELKAKVVKNWRNNPSILKSLGYFLPKE"
     gene            complement(34323..35219)
                     /locus_tag="PK898_02400"
     CDS             complement(34323..35219)
                     /locus_tag="PK898_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOU18916.1"
                     /translation="MKQNLSVSKKDFFNKETFSDYSLILIGSVIQSIGMAYFLIPARL
                     ISGGVSGLAQVINAHTGWPIGGMTLIGNIPLFIFGWRYLGKRKFAIRTVFSVVLFSFF
                     TDLLTRYQKNAYLTHDIFLNTLFGAVILGIGFGLVYRGSGTSGGTDIIGRILNQRAGL
                     PITQSYLLTDSVSVILGGLTFGWDLALYGLIAIYVSGVAAETISEGANFFRDVVIITD
                     KPDAIAEGVINVLEHSVTLITGIGGYSKEKKGVIYCVINRGEINQLKRLVVEVDPKAF
                     MVVGQANEVMGEGFQKYKYLED"
     gene            35512..36870
                     /locus_tag="PK898_02405"
     CDS             35512..36870
                     /locus_tag="PK898_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU18917.1"
                     /translation="MNCSKKKKYILPLFILLFIVGCNRSQQAAPTAEIPSEAVTRGTV
                     HNSLILVGNVEASQSSELSWKTSGVVDSVSVKVGDKVESGQVLATLLTDSMPASSIAA
                     EADKISAEDNLEELLVSETAKATAYKNLRDAEVALRDAEKLAEGLNYPIADQSEIDAA
                     EKAMIAARDAYQNAVADYEGVKLRDYYDEERMKKYETLQNALTAYAKAYDLWLYYVNN
                     APEITKKVAAASAKEAQASYEAALKEFKTYQVSFVRKQDQADAEATLEDAVTAYNARS
                     LIADLTGTVTVVNTAAGDYVKKGQSGLRIDDLSTLYISLDISEVDVNDLKDGMKAEIT
                     LDAQPTKVYQGTVRYISAFASENDYNVTYRAVVAFEQPDDLVKVGMTGEIKIILSEKP
                     DCLLVPSSAIREGDNGSYVDVVVNGTLTPVSVVTGVEENGMVEIVSGNLNEGDLVKVP
                     SA"
     gene            36952..38559
                     /locus_tag="PK898_02410"
     CDS             36952..38559
                     /locus_tag="PK898_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU18918.1"
                     /translation="MSSTGNQPVKGKKKRRKSTIVIWVLIGIIVLMLAAGGYLYQQQK
                     QVAAENASKISSQMTRNVKVEQGLISENLEEMSGTVRSNQSISLNWKISGTVSDVYVQ
                     PGDQVKKGDILAALDPATIDTSILDAAVTKEDAQEQLERLYTSSLDLATAYNTMITAQ
                     DAVKTAEDALSALGVVREDEIEIGVYYQDYLNAKSAYESALDNYNTLKVRPLDDVDRQ
                     RASQRLESARSAMESALSMYNWYNGEVDSLEKQQAEADVILKKAQYDDAVRAYNKIKD
                     GPTESQVKSLEAQVKSAEATVNTAYIIAPIDGTVAEVASKQFDVITLDNTTDEVLAIR
                     LEDLSSYSIDVSVSEMDVNSVEVGQTAEITFDAVPLKMFTGKIVSISNVGDTSGEEVT
                     YALTIRMDDTDPSVRPGMMADIQIRTKEAAGALIVPKTAVGKAQDGSQYVEKVNQDGT
                     TTRISVTTGISSDENIQILSDQIQAGDEVRETVLPDMSQNAGATGAGGLFSGMPIRIG
                     GGAAGGGAPREGGQGGPPPDGGGGQRP"
     gene            38576..39754
                     /locus_tag="PK898_02415"
     CDS             38576..39754
                     /locus_tag="PK898_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18919.1"
                     /translation="MTEKNGKNVIHTEHLKKYYVMGDIEVRALNDISLDIDYGEFTAI
                     MGPSGSGKSTLMNILGCLDRPTDGIYLLDGIDVSQMDDSELAEIRNRKVGFVFQSFNL
                     LPRSTALENVMLPILYSKNKANAKERAAEALELVGLGSRMKHKPKELSGGQQQRVAVA
                     RALVNDPEIIMADEPTGNLDSKSGGEIMELLIKLNQERGKTIIMVTHDPRIGRHVPRV
                     ISVFDGMLGTEEEQAVIRSWVKPDGEDIPDIKGKSSEKKNDANDKQTNGKNGSNGNGE
                     KHQSENTDKNDPIENKNTNDNKADDPSSDDDPPKNAGHITATEALIRAFGNGKSSPNG
                     GDHSDGDGDHGDHKAGETNGAADALANAFGKQIDEDAIRAAQSSSVKKQPKAGKGGAR
                     "
     gene            39751..40974
                     /locus_tag="PK898_02420"
     CDS             39751..40974
                     /locus_tag="PK898_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18920.1"
                     /translation="MNLGTLIHEAITNLLSNKVRSLLTMLGIFIGVGAVIAMMSVGLG
                     AKKSITDQMSALGADTLYISPGNFTQTVRNPGTLTNEDANAIAALDCIDNISPTSSAQ
                     LTVSRAGKQSSTLTVTGVFPTYLTIENYSLAEGRFITQADLDNNAMVTVIGSDTRDLM
                     FETYEEAVGNTVRIGGQPYEVVGVLKSKGDASMISSDRGVYVPLTTAQNRLITRQKHA
                     LDQIAMKINPAYSAEAAEAAVTALMRDRHDILSGEPDDFSIFNMQEIVDTMNSVMNSF
                     VIFLGSVAGISLLVGGIGIMNIMLVTVTERTKEIGLRKALGAKNRDIQLQFLVESSVL
                     SLIGGIFGIIFGYVLAYAVAAIAANAGSPMTPEINPLIIIGTCLFSILTGLFFGIYPA
                     NRAAKLEPVIALRTE"
     gene            41004..42668
                     /locus_tag="PK898_02425"
     CDS             41004..42668
                     /locus_tag="PK898_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU18921.1"
                     /translation="MALLTGLKSSGGLQPKKMLRNLALVLCLFAVIGCSAVSQKVGIG
                     STATQAGLEGIPVTRGDISAAVTLVGNVTYAQSAVILWKTTGVVQNVNVKIGDQVKQD
                     DILAELSEDSLNSSVLIAEKNLIDAQDNLTNVMESTTAKSEALSTLTAAEITYQATKQ
                     AQEALYYPRGSQLDVEMAYDAYQLALENYNYAKEDYRVVLANYKGWDDAARTTYYQAY
                     KTAYDNLISTYEDWKYLSGTPDNVELASAQGEVLSAQKAFQDALDTYSSYQTLPRDED
                     LHEANVTVHSAESSFQQRYAIAPFSGTVTAVYAEPGLYVTSGTSAFQLDDMSEYYISL
                     NTSEVDVKDIYAGQKVQITLDAIPNTTFSGIVKQVAGAGTSSTTNTVFKTLIKFDQQD
                     PQIKAGMTAEVNIFLGTKEDVLQVPLSAINQENGRSYVLKINGAETQKIEVTTGVTSG
                     NLIEVNSDELKEGDQVVVSETDLQNYLNSLTETMTTPLSAESSEGTSLAPIGTFQPVG
                     SGTPAAPIGTFQPQKTGMNPGRPAGTRPAFSGTPYPPNMNKTENNG"
     gene            42672..44177
                     /locus_tag="PK898_02430"
     CDS             42672..44177
                     /locus_tag="PK898_02430"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU18922.1"
                     /translation="MDHKITVQTKNRKNTWIILILTLVVIALGVFLYIFYVGQKASQA
                     AVAARIAAGQPSVSSVESGPMKLVIEDISGTVRSNQSVDLAWKTSGTVAAVNVSVGDS
                     VKQGDILAELDPKTLSIQMQNAQVELDDAKDDMEKLTNNAEATTSAVTELVKAQQELD
                     DAQKAYDSLDPSRAWTDAVQLAYETYLKAQNDYESALAKFEVTRDYPIDDATRIKRLG
                     DVGGYRSVRDNALAEYRRYLGEGNEMERLIREAALQLAKATLEEKQYQYDKVTKGPTE
                     AEINAAQAKIDAAQQKLNSSKLIAPFSGVVTQLETKVNDVISETAAASATKTTAIRIE
                     DLSTFYIDTTVNELDVNSIQPGQAAEIQFIGIPDKTYHGTVTTVADTGVSSGRSVTFN
                     VTLKIDDADELVKSGMTADLSMTIANIPETLYVPLSSVLLKNGQPSVNLKKMDGSFEW
                     VPIETGLVSGTKVQVLSDNLKAGDEIEADVNVDSTDTNDNRMFPMGFGGRM"
     gene            44363..45181
                     /locus_tag="PK898_02435"
     CDS             44363..45181
                     /locus_tag="PK898_02435"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOU18923.1"
                     /translation="MKILIACDMEGITGVTRWEHCDPTHPEYQRFRKQMTMDVNAAVE
                     GAFAGGVEEVQIADGHSQGQNLLIEELDSRAQLNCGTTSPWSMMNGIDSAFGGVFFIG
                     YHAMAGTANAILAHTWSTQILNWKINGQKIGEFGMNALIAGWFDVPALMVSGDQSLAT
                     EALRFVPDIDCAVVKEANGYFSGKCFSPAKTHERILECAQLAVQKKREGRGPQPLKMN
                     APYEVEVEFTQPYMAETASRLTGSERTGGRSVTITVPDIPAAFLAARTLCNLAV"
     gene            45242..46138
                     /locus_tag="PK898_02440"
     CDS             45242..46138
                     /locus_tag="PK898_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU18924.1"
                     /translation="MKYFSDTSNPEQNNRKTYQALIITLAGNLILALSKLYVAHVSGS
                     SALRADAYNSLSDVLYSVTLVIGMMIAIRPADISHPQGHRRFEPLVGLLISFSMAWAG
                     YQAISESIDKIRSGPTTFDLGLPILVLFFSAVIKGVMYWLIHRIAIDLSSPTLKTAAT
                     DNLMDTITSFTAIIGIYLSSIWSPLADPIAGILVSVWIFRAAFEALQENLGFLTGAGA
                     SEERRQQFLDIIRSVPGVANVHQLFAEHVGSTFVLDVHINVDGNTPLYQVHRIETEIE
                     EKLTAIPDVERAYIHVEPLGYE"
     gene            46314..46751
                     /gene="dtd"
                     /locus_tag="PK898_02445"
     CDS             46314..46751
                     /gene="dtd"
                     /locus_tag="PK898_02445"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOU18925.1"
                     /translation="MKAVIQRVSRASVTVDGRLISRIGTGLLVLLGVEEGDTRENALA
                     MAKKTAALRIFRDENDKMNLSVRDCAGEAIVVSQFTLCADTRKGNRPSFIGAAAPEIA
                     APLVDLFAESMREQGVPTQQGLFGADMKVELLNDGPVTILLEN"
     gene            46754..48616
                     /gene="uvrC"
                     /locus_tag="PK898_02450"
     CDS             46754..48616
                     /gene="uvrC"
                     /locus_tag="PK898_02450"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU18926.1"
                     /translation="MEISPKIQGILETLPNKPGCYLMKNADDKIIYVGKAINLRHRVR
                     SYFQNSVKHTVKTRQMVSHIDHIDWIVVESELEALILEMNLIKKNRPQYNIQLKDDKR
                     YPYIKIEFQNPYPRILVTRQMVQDGSRYFGPYTSVWAVHQTLDLLRRIFPYLTCDREI
                     TGQDSRPCLYYDIKLCSGPCINAISQADYYAMMEDIASFLEGKTADIVNRLQKQMSDA
                     AENLNFERAAALRDQIQAIDRVVEKQKIVSNDDTDSDVIAIARSEKEACIQLFYIRNG
                     KLIGQEYFIMKGTEDEADQEVVSEFIKQFYSDAATIPPQMLLPVELEEHRIIQQWLRQ
                     QRGGKKFTLKVPKTGNEKDLVNMAAENAIETLRALKTQWENDTNKQSEALTELQSALQ
                     LPEPPNRIECYDISNTQGTLSVGSMVVFEQGVPKKNLYRRFNIKTVEGPNDFDSMTEV
                     LTRRMKRWQSASEKDNKTGGKIDPSFSVLPDLIIIDGGKGQLGRAVEVLKDFDLLDRV
                     HVVGLAKREEEIFMPFHSDPIILPRNSEGLYLVQRIRDEAHRFAITAHRGRRTKEGLA
                     SQLEKIPGIGPVKRKALLKKFGSIDAIAAASKEELMLTDGITSSLADAIRDYFG"
     gene            48702..49346
                     /locus_tag="PK898_02455"
     CDS             48702..49346
                     /locus_tag="PK898_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOU18927.1"
                     /translation="MMEKTAEQIRYDKLGETVAKALRKRFFDAWYFDNKEAAVAQALS
                     LIPEGTSVSWGGSMSIVEVGLKDKIQNGKYKLIDRDTAKTPEERIEIMRQALLADTFL
                     MSANAISEDGQLFNVDGNGNRLAALLYGPKQVIVIAGVNKIAHTAADALERARTIAAP
                     TNISRFDVATPCKITGTCSDCTTAESQCAQIVQTRICRPAGRIKVILVGTKLGY"
     regulatory      49466..49569
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            49633..50847
                     /gene="metK"
                     /locus_tag="PK898_02460"
     CDS             49633..50847
                     /gene="metK"
                     /locus_tag="PK898_02460"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOU18928.1"
                     /translation="MSTFMNSPKYLFTSESVTEGHPDKICDQISDAILDACLEQDPYS
                     RVACETSVKTGFVLVYGEITTQAFVNFDELVRKVVLDIGYDSSEKCFDGHTCGILSAI
                     STQSPDIALGVDKAQEAKRGEFGEVDFEEVGAGDQGMMFGYACNETPVYMPMPIYLSH
                     RLTEKLSEVRKNGTLPWVYPDGKSQVTIEYSYGKPKRVDTVVISTQHSASMRDKHDEI
                     EAAIRKYVIDPVLPKELVDRDLKVFVNPTGEFIIGGPQGDSGLTGRKIIVDTYGGMGR
                     HGGGSFSGKDCTKVDRSASYATRWVAKNIVAAGLAERCEIQISYAIGVAKPISINVET
                     FGTGKISDEEIVRLIDRNFDLRPGAIIKRLNLRRPIYRKTSNYGHFGREDADFTWEKL
                     DMAEALRRDAGL"
BASE COUNT        14885 a        10665 c        11751 g        13643 t
ORIGIN      
        1 ggacactcgt tactccttaa gtgaatcctc ttttttgtta aattcgaaat tttttaaacc
       61 tttctttttt ccattacgcg aatttgcctc aagagacttt tgaggcaaat tccatttctt
      121 catttcccct tccgttataa tagaaaaaaa tgatttacct tttgaaaggt aaaaaataat
      181 taaatgcgaa atatttttac gattttattg cggtttttag ggctttgggt tttcaatgca
      241 atctgtcttt tagcttgtga atgggttatt ccgggtatta cgatcatccc gtatcagggt
      301 gtcccttatg cggtaaccgt gatggaagtc agtctgattt tttccataat gaacttattc
      361 gtacgaccgt tgattttatt gttcacttta ccgcttaacg gactgacatt cggcttattt
      421 tctctggtag ttaatgcttt ggtgctgttc cttgcttcag aatttactcc tttctttaaa
      481 atcgaatacc ctatcgctgc ttttctatcc ggctttttat tcgccttggt aaacatcatt
      541 atcacatctc tgattccgat tgacgaagat tttctttatt atgatatttt ggtaaatcgt
      601 ttcagaaaaa gcggtgaaaa aattaataag gacaaaaagg gaattatcgt catcgaaatc
      661 gacggactgg cgtaccctcg attaatcaaa gctattgaaa aacggaaaat gccgtatttg
      721 aaagaattga ttgattccgg aaaatttacc atttccgaat ttgattgcgg gatcccttct
      781 cagacttctt cctgccaagc cggtattatg tatggtgata accaaaatat ctgtgcctat
      841 cgttggtatg acaaggaaca gaagagaatt atttcgtcca gcagctttca agatacaaat
      901 cagatggaac aggaaatcct gcgccgcaaa aagaacggtc ttttagacgg agggatgagc
      961 atcaataacc taatgtccgg gaatgcggat gtttccttat ttacggtcag tgcaatccaa
     1021 ccgaaaaact cggaagaatt aaataaccgt aacattaatc tttatttttt ttctttaaga
     1081 ccgtatttat tgacaaaatc tatcctattc gccctggcag atgcagccgt tgaagttatc
     1141 caatatgtct gggcatgtct caaaagaaaa gaaccgagac ttaatcgttt ggttcatttt
     1201 tatccgctca tccgcggatc aacaaacgtc cttttgagag atgcatccac cgcattaacc
     1261 atcaatgagg cgatgcgagg gggaccggca agctatacca cgttttatgg ttatgatgaa
     1321 atcgcacatc attccggtcc ggatagtttt gaagcatatc atgcgctgtc gggaattgat
     1381 cattcgatca aaaaaattca tcatactgcc gaagaaaact cggacagaga ttatgaattg
     1441 ttcgttattt ccgatcacgg tcaatctttc ggaaagacct ttcgccagcg ttacggatat
     1501 agtatcagtg attatatacg gaaattagcc ggtgaatgtg cccgcttgaa atcggaatcg
     1561 caagtaatcg gtatcgggaa cagtgcagct gataatgaat ccagcattcg tgcggcgatt
     1621 tatgatctga ctgctcaaaa gaaaacgcaa cagatcccga ttcccaaaca gacattaaat
     1681 aaacttgata ctgttcttga agaaaatgcc gatgaaacac gagctaaaaa acgaccggaa
     1741 gacaatgata tttggattct tgccagcggg aatttagcca atgtttattt ttcattttca
     1801 aatcaaaagg tcgattatgc cgaaatagaa agccattatc ccaatttatg ctatcgcctc
     1861 attcaacacc ccggagtagg tcttatggtc gttcatcaaa ataaaacggc gattgcaatc
     1921 gggaaaaaag gcagccggaa tttatccacc ggcgaaatat caggacaaga tccgttggca
     1981 aattatcagc ccaccgaaat gcgttctcgc caactatcct atttggctga gtttccaaac
     2041 ggaggagatt taattctttt ttccaccttt tatcccgacg gaacggttgc tgcctttgag
     2101 gaattgatcg gttctcacgg agggattggc ggagagcaaa atacagcgtt tatgtttcat
     2161 cccataaatg ttgaagtacc tgagaatata ttcaactcaa aagaagtcta tgccatcttg
     2221 aagggacgtc gtgaattaga tgcagagatc gataaatttt cggaagagaa aacgaaacaa
     2281 aataacgggt ccgaagattg gaaatgggat aaattaattg ccggcatacg agatacttca
     2341 aaatggacca aatacatgcg ggaagtaatt ctctttcaat cgtcaattta taagaaaatc
     2401 agtaaagata cgagtctaaa tggtccggct gtgttaatta tgatgatgtc gctgttatca
     2461 aatatgaccg ttgccggaat tcttttccga tccttaaaag ggctgtatct ggggctgatt
     2521 atctggttag tctcatggag tattgttatc ggagcttcct atggggcaac gcttgcgttg
     2581 aggcagaata tcattttttc cgattttctg cgcggcatga tattttgtac cgtttttgat
     2641 gtccttttat ttggcgcttt gatccccggc tacgaaaact tctggattgt tattattttg
     2701 gttacccgtc tcatctctgt ctcgcttgcc attaatggta tttgcaacat acaagggaaa
     2761 agatctttat tgattgtgcc tgttgttttg tttatgctga tcttgttagt gattacgact
     2821 tatgttattt tagaaagcct cggatacatt ctcaaaatcg atcccctgat tgactttatc
     2881 aaccatatta acgaaatagt gggaatttca tgacaaccga tataaaaact attgaaaata
     2941 ctattttttc gaagtctttt tattcttggt ctgcaagcga gataatggga ttatcgcgaa
     3001 aaattattaa aaatcaatct gaaattctgg caaatcatcc ggaactcgtt caaaaacgaa
     3061 tcgcaatttg cggaacatgt aatattcggt ttatatcttc cgggttaatt ccgtttttaa
     3121 tggccgaagg aattttcccg cttatttttg aaggaaaatt caacggactg agcagtgaaa
     3181 tcctttcttc cgattcggac ctttatgctt ttgatccgga ttatctgatt gcaatcactg
     3241 attatcgaga cattcatgaa ttcccacctt acctcacttc cattgaaaaa gtaagggaga
     3301 cggcgaatcg gcggatcgaa gagtcgattc atttctggga atattttcat caacgctgca
     3361 gaagcaaagt ccttttgacc aatattgcca ttcccaaccg tcatatactc ggcggactcg
     3421 aagcgaacct cgtttccggt cgagatgtat ttttaagaat tttaaatctc acttttttag
     3481 aaaagaaacc gacattttta acatttcttg atcaggaagc cctttcggcg gatttcggga
     3541 aagaccgatg gtttgatgat tccgaatggt ttatcagtaa acaaccgttt gcattggaag
     3601 caataccgat tttttcatta acaatcgcta aacagatcgc agccgatatc ggaaaaattc
     3661 gaaagtgtct catcacggat ttggataata ccctttgggg cggaattctt ggcgatgatg
     3721 gtatcaacgg gatcaacctt gatccgaacg atccgatcgg agaatccttt cttgattttc
     3781 aaaaaactct gaagctgtat aaggataggg gaattttatt ggcggtttgc agcaaaaatg
     3841 accctgttat cgccaaagaa gtattccaaa aacacccgaa ttgtattctc aaaacagagg
     3901 atttttccgc attcatcgcc gattgggaag ataagccaat caacattcaa aaaatcgcat
     3961 cgcagctgaa catcggactc gattcactcg tgttttttga tgataatcct gccgaacgag
     4021 atttggtaaa aaaagttttg ccgatggtgc aagtcatcga tgttccggag gacccggccg
     4081 aattcagcat ggcattaatt cgttcccatt gttttgattg gggacaatta acggtagagg
     4141 atctgaaccg ttccgaaact ctccgagcgg atcgaaaaag gtcagagctt ggtgccatag
     4201 cactcaatta tgaagattat ttaaaagcct tggaaatgag gataagcatt gcgaaagccc
     4261 tgcctcatga gctgaatcgt tttacgcaat tgattaataa gacgaatcaa ttcaacttgc
     4321 gaacgcgacg ttacaacgaa tctgaaatca ccgccatggc aaccgatccg gataaagttc
     4381 tcctgcgcgt gacgctgcaa gataaattca gcgattacgg aatcatttcg gcaattattt
     4441 tgaatcttga tcactcacgg aagaatattc ttatcgatac gtgggtaatg agctgccggg
     4501 tttttaaacg cggaatcgaa gatgaagtga tgaactattt gatcgctttt gcaaaagata
     4561 acggttttca aacagtcgaa ggtgaatatg tctgcagtcc gcgaaacggg ttggttgccg
     4621 atctttaccc ccgctatggt tttaaaatgg tacaacaaag tgacatatcg aaaaaatata
     4681 tattggacat caatgattat gttccatgtg aaacatggtt caattttgaa ctcaaaggga
     4741 aagaatcatg accgatctcg aaaaaataca aagtattatg cgcgacattt tcgataatga
     4801 gacactcgaa attgatgaaa gtacgagtgc ggaagatatt gccgaatggg attctttttc
     4861 aaatattcaa cttattgcgg ctgcggaaca acattttgcc gtgaaattca cgacacagga
     4921 agcaattcat attcggacgg tcggagatat attaaatctg atcagacaaa aaaagctccc
     4981 ggttgataaa ttataaagat cgatttatat cgcgaataag cgacttttta ccgttaatct
     5041 tcatataaat atttcagatc agaaaatata gtaaccggtt acccgattga aagacactct
     5101 tgatagactt tgagatatgc catacccatt tgcaaaatcg aaaaatgttc gataattctc
     5161 tgcctcgatt tgaatcgcaa aacatcttgt tcttcggcgg acaaaaagac cattttccga
     5221 atcgcttcgg caagcgccgc cggattattt ttagggacca aaataccgca atcatcaaga
     5281 agatatggag catctccgac atcggtcgta acacacagta atccgctgct catcgcttct
     5341 cccaatgcca aaggaaaagc ttctccgatc gatgacaatg taaaaatatc tatagcattc
     5401 agtaacgccg gtatatcatc ccgttcacct aaaaggagca tacgggattc caatccggca
     5461 tcgcaaatcc aatcgcgcaa ctccttattc tcccaattta agccggaccc gaccaacaga
     5521 aagcgaaaat gaataccagg caccttctgc agaagttctt tccaagcttg gatcaaggtt
     5581 aaatggtctt tttgtgaatc aaagcgggaa atgtttccga tcagaatatt gtcatccgat
     5641 attcccaatt cgcatctttt ctttctccgc atttccgagt tcggtttaaa ccgatccgta
     5701 tcaaacccgt tgggaatgac cgtaataata tcccctcgat accccatatc aatatgattc
     5761 tgctttccgc tgcaggaaca ggaaatgatc cggcgaggaa ccgattggga tagatgtgca
     5821 agctgtgaaa caaccaaccg ggtcgaaagt ttcacgttcg gacttaaagt ggcattatgc
     5881 agaccccata taaccggaat ttgcagcttt cgaccgacaa gacttgcata caagtcagca
     5941 tgataaagcc aagaatgaat aagatcggga cgatcgtctg cagctgtttt tttaagtcgc
     6001 cgaaaagcgc tgtaactggg aacagccggt ttcatgttga gacaagtcac acaaatgccg
     6061 ttttcttcga taattttggc aaccggaccg gtacttctca aacacacaac agattgttcg
     6121 ataccgccgg atttcagaac cggaatcact tgtgcgagca ttttttctgc accgccggta
     6181 tttaagtcgg ttataacgtg caatacttta atcattctga tttatattgt aaaggaaatt
     6241 aaccggcgag cggaatatcc gaataaattg gttggcatgg ataactattt ttttacaaat
     6301 gatcgactaa tcgcccgatt taaattcatt ttcctgattt cataacgaat ttttcaaccc
     6361 gataacaaac ggaagtcaat ttaaaaaggt tttatggttt ctttgggaac agccggatta
     6421 tttgtttttt cttccatcaa aagaatattt ttagataatt tgtcaaatcc tttcgacatg
     6481 tttttcagca acttgtcatc aaatccatca aaaagttggt tgataaattc tttgtccgat
     6541 tccgcttgtt gttgtagact gtttttccct tcttccgtca aggcaactct gacaattctg
     6601 ccatccgtat catctttgct taataccaaa taattttgtt tctcaaggag taaagccatc
     6661 ttttttatat tttgtcggga acttcccagt gcttccgaca attccgtaat cgtcatagga
     6721 gccggatcat tttttgaaag cacttccagc aattggaatt gtttaatcgt tattccggtt
     6781 tccagtcgat cacctaatat ttgaatttta ttggaaagcg tgaatatttt agacaatacg
     6841 attttgccgc gattcgtttt acccatatgt cgccctcctg tcaaaaggga acctattatc
     6901 tttataaaac aagtatacct aatgaagcgc cccgtaacaa tacattaatt ttttacgcct
     6961 cttgctttta ttccgttttt catcttcatg gtgcaatttc aaataaggta taaggtccca
     7021 caaatcaaat agattgaccg ggccttcata atcaaatcaa ctccataaaa atcgattcac
     7081 cggtttatcc tgtttttacc gattcccaca tagatacctc attttgtcat cgatctttaa
     7141 atacgacacc ctttcttgac tacccgttgg cattcattta tcggaacatt caattgtgtt
     7201 agaattttcc ttatgcgaga tcaagaaaaa attttgttgg cgtccggctc ccaacgcaga
     7261 agagagttgc tctcatggtt aaccgatcgt tatgaaatta ttccctctga tattgatgaa
     7321 agtccgctgc ccggagaact tccgcaggtg tattgtcaaa gaatcgcgga acagaaagcg
     7381 cttgctattc cttccagaac ggaaacgcag aattcttttg ttcttgcagc cgacaccatt
     7441 gtttattcac aaaatcggat catcgggaaa cccaaagatg agcaggatgc atttcaaata
     7501 ctgacctttt tacaaggaca aattcatcat gtctgtactg ccgtaacatt atatcggaac
     7561 gggaaaactg tcagtacttt atgcgacacg ttagtccgta tgaatccctt atcccaagcc
     7621 actatccgat cttacatcgc aaccggtgac cctatgggta aagcgggtgc ttacgcaatc
     7681 caaaataccg attttaattt ggtggaatcc gttgaaggat gttacacatg cgttatcggt
     7741 ctccctttat gccatgtgga aaaattgttt tcattctttg actattcctt tgagaagaat
     7801 gttatcgaat gttgcaaaag ccatatccat tacaattgtg tagtggatca gtctgaaatg
     7861 ttacggaaat ccttattcaa tcaaaaaatt atcgaacgaa acgtatgaaa agattcatca
     7921 tttgctttat ttttctttgt gtttttaccg ccggatgcgg tccgaaagtt tccaacgaat
     7981 cgacaaaaac gcctgagata accacggtga tgccgagtga tacacctctg ccggaaccgg
     8041 tggttatgat aacctccgct ccggaagcga gaacaactgc gaatacattc cttgaatttt
     8101 ggaaagcgga tgatttcgtc agtatgtata acatgctttc ccaagtcagc agagatgcta
     8161 ttccgttcga agattttgaa accaaatata aagaagctga aaccaacttg actttacaaa
     8221 gtttaaattg tgaggtgacc tccgctttaa ctaaccctaa aaccgcgcaa atcggatatc
     8281 atgcgaccta tcaaaccgcg ttagcgggaa ctttctcgcg cgatatggta atgaatttgg
     8341 tttttgaagc gaatgattgg aaaatacagt gggatgacgg gatgatcatg cccgagctga
     8401 gcggtggaaa caaaatcgaa atagatatcg atgtaccgac gcgaggaccg atttatgaca
     8461 tcgacggtgt tccgcttgcc gcagaaacag aagcatatgc aatcggggtt gtgcctgctt
     8521 ccgtcccatc gaaccgttgg aatggcttaa ttaatgaact ttcacgactg accggtaaaa
     8581 cgacttttgt gatcacacag ctgatggaag aagctaatca gtatgattac gtggtcatcg
     8641 gtgaagtccc cgcagcggtt gttgaagaac gaatggaagc catttccagt tatgaaggtg
     8701 tttatctcaa ttcatataca gcaagccgtt tttattatga gggcggatct gctccgcatt
     8761 tggtcggtta cgttcaaccc atcggcgagg atgaagccga aaccatgaaa agacaaggat
     8821 atcggattga tgaacgaatc ggaagaatcg ggatcgaaaa atggggtcag gatcgtttgg
     8881 atggcgaacg aggagcttct gtttatgtcg ttgattcagc cgggaacccg atcacccgtt
     8941 tatataaaag cgatcccgtc cctgccgaaa cgatctatac aaccttcaaa gaagatttcc
     9001 aatatgacct tcaaagggcg atcgccggat ttagaggcgc aattgtcgtg ttggagaggg
     9061 ataccggcag agttttagga atggcttctt caccgacttt tgacccgaac ttgttggatt
     9121 ttaataatta caattctttt tacagtgcaa atttgcttta taacgatgac cgtcctatgt
     9181 acaaccgtgc tacccaggga caatatcctt tggggtccgt gtttaaaata atcaccatgt
     9241 cagccgcttt ggaatccggc gtatatcacg tcaatgacac ttacgattgc ggttatgaat
     9301 ttacggaatt gcccggaatc actttaaccg actggacact cgatcatgat gtcgcggcaa
     9361 gcggtaaact cacgctttcg gaaggtttga tgcgctcctg taatccttgg ttttataaaa
     9421 tcggtctgga gctttttcgg caaaaaggag cggattattt acccagtatg gcaaggggat
     9481 tcggcctcgg atcgcctacc ggcatcggtg aggtggaaga ggaatccggg aatgtcgaaa
     9541 cacctcccga tgaatattca tccgttcaaa tgggtatcgg acaagggact ttattagtca
     9601 caccgcttca agtcgcccgt tttgtggcag ctgtcggtaa cggagggaca ctatatcgtc
     9661 ctcaggttgt agaacagatt gttgattacg aaggaaatgt tacttacgct tttaaacccg
     9721 aagaacaagg caaattaccg gtttcaaagg aaaatttgga attagtccag caagcgatgc
     9781 tgagtgtgac gaataatccg cgcggtaccg cctattcgac ttttaaatat attaaatatc
     9841 cggtttacgg gaaaaccggt acggctcaga catcacccgg agaagatccg catgcttggt
     9901 ttgccggata taccgatcga aaactggaag gaaaaccgga tatcgcggtg gccgttctgg
     9961 tggagaacat gggagatggt tcggtttatg cggctccaat tttccgcaga gttcttgaac
    10021 tttattttga cggaaaagca tccatgcttt acaactggga agcgggggtt tatataacga
    10081 agacaccgac tcctacgcca acgatcacgc cgacaccgga accgacgaaa gcgccttggc
    10141 aaataacgcc tgaggttacc ccttaaatcg acccgcgaat gactctgcaa ggacgcgtga
    10201 ttcgttatca atccggattt tataccgtca tgaccgatga gaggatcgtg acctgtaaaa
    10261 tacgcggtaa attaaaggaa aacatccgtc aaacagattt ggttgccgtc ggtgatttag
    10321 tcgagtttac gcagtttcaa gatgggacag gtgttatcga atgtatcctg ccgcggaaaa
    10381 atgaactgat tcgaatgatt tccggtatca aatcggaata tagacaagtt ttaatcagca
    10441 atctggatca aattcttttg gtatttgccg ttacacagcc tgagccgcgt ttgagaatgt
    10501 tggaccgttt cttggtgatc tgtgaaaaac aaggcatacc gccgttaatt gtggcaaata
    10561 aagttgattt atcaggtatg gatcaggcga aatctgtatt ctccgtctat tcggatatcg
    10621 gctatgatgt tttatatacc tcgacggctt ccgatatagg aatcgcggat ctaaaagaac
    10681 gattacaggg aaaagttacc ggtttggtcg gcccctccgg cgtcggtaag tccagtttga
    10741 ttaatcgaat cgatccccgc ttgaacttga aagtcaatga aatcagtaat tacaatcgga
    10801 aaggaaagca tacaacggtc gtgcgggaaa tgttcccctt aaaagagggt gggtttgtcg
    10861 cggatctacc gggactcaag acgcttgcac tttgggatat tcaaccggaa gaattggacg
    10921 gttactttcc cgaaatcagt ccattggttt caaaatgtct atacggagat tgcacacaca
    10981 gcccttcgga agttggttgt gcggttcatg aagcggtaaa agaaggcaaa ataagccggg
    11041 aacgttatga atcgtatctt cggatccgat tcggcgaacc ggaatagctc cccttttttc
    11101 cggtttcata tatatttttc tatcatttgc agtcaaacag aaataatact gttagaatcg
    11161 agctatcatc cgattcataa aaggattgcc atgccaaaaa gactcacttg gactgaatta
    11221 atacttaatt taaaatctcc ttttactgtt tcttacggaa gttccgatac acggaccgct
    11281 tattggatcc ggctcgaaga agattccggt tggggagaag ggacaatccc tttttattat
    11341 ggcattgatt ttaaagacat gactgatttc tgggatcgtt gtgcattgcg ggaagatcct
    11401 tttccggaaa caattaatgc aattcatcca tggatcggcg acgatgcacc cgcacctgcc
    11461 atcgcggctt tggatatcgc ttttcacgac cggatcggca aaaaaaccgg aaaagctatt
    11521 tatgaacttt atgatcttcc ggccccccat ccgatgacca cctcttatac aatcagtatc
    11581 gatacaccgg aaaacatggc acaaatggct ctcgaaatca atcggtaccc gttgatcaag
    11641 attaagctcg gttcacccgg ccaagaagat ttggatgaag cacggatagc ggcaattcgg
    11701 accgtacgtc cggatgcaaa attaaggtta gatgccaatg ccggttggaa gacggacgat
    11761 gccgtcaaat tagtaaaacg tcttgaaaaa tataatatcg agctgatcga acaacccact
    11821 gcgaaatttg atatcctcgg gatggggcat gttcaaacag aaacaccgat tccgattgtc
    11881 gcggatgaat ccctgcaaac aatcaatgac attgagaaat tgcatgatgt cggagttcga
    11941 ggaatcaata ttaaactgat gaaatgcgga ggtattgccc ccgctgttga gatgattcgg
    12001 aaggcccgtt catacggaat gaaaatcatg ctcggctgta tggtcgaaac ttccatcggt
    12061 gtcggtgcga tggcttcttt gatcgggttg gctgattgga tcgatctgga cactccattg
    12121 ctgatttcca acgacccgtt cagcggactt acttacaccg aagacgcaac actgatcccg
    12181 ccggccggac ccggattggg attgaaactt aaaaactccg aaaggtaggg aaaaatatga
    12241 catttcaaaa aatagaaacc atccttgctg acgttaagaa gaattttttt aatgaaccga
    12301 tgctgaccgt ttttgacatt caacccgaaa tcatttcatc aaatgaatta aaactgaccg
    12361 gtaaaatact cgagcaaaaa gatcttgatt tccttaaaaa tgaattggag aacaagattc
    12421 cggattggaa ggtcgattac aaagggataa aagtattact gaatgaaaaa acgacgattt
    12481 tacgcgtgag caagaatctc accgatatgc atcgtgaaaa atcttttcta tcggaactca
    12541 ccaatcagat gctttacgga gaccccgttg ctgttctggc tgacgaagga gaatgggttt
    12601 tagggcgcaa tatgtcagac gggtatatca gttacactta taaaaaatac ctcggcgaaa
    12661 taccatggtc ggaaccgacc catattgtca gcgaaccggt ggcattggct tacaacaatc
    12721 cgaatcggga agagttggtg acgcgagtgc ttggcggaac cgagacggtt atattcgatg
    12781 aaaaaggaga tatggcattc atccgggcgg atcacaacgg atgggtgaag aagacttctc
    12841 tgattcccat ttcggatctg ccgaccgatc aggataaaat tcgaaatcgg gtttgtgaaa
    12901 atgcgaaaag tctgatcggt gttccctatt tatggggcgg tacttcggcg tgcgggatcg
    12961 attgttccgg attggttcaa tggtcttatc gattatgcaa catccggctt cgcagagatg
    13021 cacatatgca gcttattccc gaaaaatgtg ttgagcctcc gtttttaccc ggagatgttg
    13081 ttctttacgg agaagaagaa aacggtaaga aatccatcac ccatgcatcc atcagcttgg
    13141 gaggatggac tgtcattcat tcatccagat cacaaaacgg cgtttatatc gatgatgcct
    13201 taatcgctcc ggctttaaaa ggaacttttg cggctgccgt acgatatatt taaatcattt
    13261 gattcggatc aaaataattg tcattttttg ttgatcggaa tattatgtaa ctggttactt
    13321 tttatgaatc aagcagccat ttaccgagat ttccgaaaac atcatagact ttctgatagt
    13381 ccgaataatt attgcaaagc aaaaaaatcc gacagcgatc ggtaaatccc cattcattga
    13441 tcacattatg caaaccgccg gatttatgat aaaagtctac cgaatcacct ttgagattgc
    13501 gggtgaaaag tttatccgcc tggcaaaccg ataacatttc agatatattc ttctttgctt
    13561 cagccgggaa agagtggaac agatcgtaac agtgaatcat atccgcagtt gtcgtccaat
    13621 tgtcataacc ccgtgccaaa tcgggttttg acatcatttt ccttcccaga tgggtatccc
    13681 gaaagtgaaa agtctccgaa aagatgtgat taatcctttc aaaacccaat ttattaatga
    13741 tcaaattcgt acagtaatta tccgaagttg caatcatcat cagcaataaa tcatgatatg
    13801 gtaatgcttt ctgaaagaat aaataataaa aacccgcccc tttgacgatc ggttcagaag
    13861 aagggtcaca aatttcatcc gaagaaacaa atccctcctt ttccgaaaca taccaagcaa
    13921 ataataacgg cactttgata accgatgctg ttagaaatct gtgagattca tcccgattgg
    13981 aaacagctat cggatgattt tcttgatccg aatagtagaa agtatattcc ccatccagtg
    14041 ttgaaagttc cttttcgatc cgttctctgt ccatgataaa gtcctccgct ttctgattat
    14101 aattttgatt atagagaatc ctttcatgaa acggagataa ttcattggta tatcatattt
    14161 gcactcaaac ctcatatgaa tcagtcaaaa ttcggggaaa atattccccg gacagtctgg
    14221 ggaaagaagg atttattcat ttctcttcaa tcagacaaat ccttgagacg gctaattcct
    14281 tctatcgggg tcaggatgat ttagttttat tggaagtcga tgagcaactt accgatcaaa
    14341 ttcgatttga aaggcctgca gatcaccgtg agggtctttt cccgcatttt tacggagaat
    14401 tacccgtaga actcattcga agggttctgc cgttcccgca tcaagcagac gggactttca
    14461 aattgcccga agaaatcgcg gaatcataaa tgcttgaaga gtctcaattc aagttgatcg
    14521 atgaattaat tcgagccgcc ttaaccgctg tcgatcccga gaatgttgta aaaaatgctt
    14581 tggatttgaa gaattcaatc cttaagatcc aaaaccaacc gtttgactta tccgaatttg
    14641 aacatattta tttgatcgct ttcggaaaag cctcactgaa aatggcaaaa gcgacggatg
    14701 atttaatcgg ccgattcatt accgggggaa ttatcgtatc aaaaaagaag aattcggaaa
    14761 ttcgattgcc tgataaatat acctgtctgg tgggaggaca tccgatcccg gatcaaaaca
    14821 gccttgaaag tgcacaaagg atcttaaatc tggtgaaaga tgccgggaat cgagatctga
    14881 taatatgctt gatctccggc ggagggtccg cattactgac tctgcccgag aacggtatat
    14941 cgttaaagga tattgcagac ctgaccaagc tgcttttgga aaacggagcg aatatccagg
    15001 aaatcaacgc catccgaaaa catatcgacc gaataaaagg gggaaaatta gcaatagcgg
    15061 cagcaccggc acgaatcgta actttgattc tttccgatgt gatcgggaat tccttggaag
    15121 ccgtcgcatc cgggccgaca gtcccggatt caaccaccta tcgagattgt cttgaaatca
    15181 ttcaaaaata tcatctctcg gaaaaaatac cggctgcgat ctatgaacga attctgatcg
    15241 gagccggcgg tgacattccc gaaaccctga aagatgattc tccgatcctg aaaaatatct
    15301 cgaattactt gatcggcgat aattccaccg ccatcagtgc catcgttgaa aaaggaaaat
    15361 cgctgggctt aaatgtctcg gtaattccga aacccatcat aggtgaagct cgggaagccg
    15421 gtcgtttttt ggcagctgaa ttaaggaaac cggttacctt taaaccttct cttctggtcg
    15481 gcggaggtga aacaaccgtt acaatcaaag gaaaagggtt gggcggaaga aacttggaga
    15541 ccgctttggc ggctgtcaaa gatttggcgg ggattccgaa tatcgctctc gttacgcttg
    15601 caaccgatgg tgaagacgga ccgaccgatg ccgccggtgc gattgttacc ggcgaaacct
    15661 tttcggaagg catgaaaaaa gggttggatc ccgatctgtt tcttgataat aatgattctt
    15721 accactattt tgaacaaatg ggaggattaa tcaaaaccgg ttcgacgggt accaatgtca
    15781 acgatttggt tttccttttt cacttttgaa tttaatccga aaaatcgatg taattttctt
    15841 tatcgcttat ttaaaacaat tttttttcct atttgatcct tttagaaagc taaacgttcc
    15901 ttccgttttc gggaaaatgg aattttaaaa tcttttatat ggattcattt ataatgaatg
    15961 ggataagcgg aggaaaaatg aatcagggaa aaaagtacta tcaaaaatca tggagtttag
    16021 aagatcttta ttccggcaca aatagtgaag aataccaagc tgcgatgaat gagttaggcc
    16081 atttaaccga tgaaatccaa aattatcgcg aaatgctttc tccggaaata tcggtttcgg
    16141 atttcggcac catattgcag aagctggaaa agtttcatga aatcgcttcc cgattgcttt
    16201 cttatgcgga gcttttgttt tctgccgata ctcaaaacag cgaagcgatg agcttggtgg
    16261 gacaaaccga agcgattata accgaagcac acaataaaat tatcttcttc tcattatggt
    16321 ggaaatccat atccgatgat gaggctcaaa gattgatcaa agagtttcct gattatcaat
    16381 attatctgaa acaattacga aatacgaaac aatttaccct tcccgaattt gcagaacaaa
    16441 ttatcaatat taaagatatt tccggaaaga atgctttgaa caccatatat gaatcgctga
    16501 caaatcgtta tcagttcaca ttgaaaattg acggaaaaag caaaaaagtt tcacggagcg
    16561 gtttgatgca gtacgtcaga aatgaaaatc cggaaattcg aaagcaggca taccgggaac
    16621 tttatcgcgt tttcggggga gacggcaata tattggggca gatttatcaa agtatcgctc
    16681 gggactggta ttttgaaaat gttaaaatca gaaaatatcc ttctccgata tcggttcgaa
    16741 acatcgaaaa tgacattccc gatcaggttg tcgatttact cttagatacc tgtataaaaa
    16801 ataagtcggt atttcaacgc tatttcgcat tgaaaaagga aatgcttgcg cttgaaactt
    16861 tcactcgata cgacatttat gcaccgcttc aaaaagagga gagattgatc ccgtttgaag
    16921 aaggcctttc gattgtacaa agcgctttta ccgactttga tccggaattc gcagccatgg
    16981 caatgcgtgt gcttgacgaa gaccatattg acagtgcaga tcgccgggga aaaagggacg
    17041 gtgctttttg tttgacaact tctcccaaac tgactccgtg ggtgctggtc aattatcagg
    17101 gaagaatctc cgatgtttcc acattggcac atgaattagg ccatgcggtt cattcactgt
    17161 ttgcgaaccg gcagaatatt tttcaacatc aggcatgtct gcctttagct gaaacggctt
    17221 ccacttttgc ggaaatgttg ttagtggatg ccttattgaa agaagagaaa aatccggata
    17281 ttcgaaaaga actgattatg cagcaaatgg atgatttcta tgctacgatc caaagacaag
    17341 catattttgc cctattcgag aaaaaagctc atgaaatgat cgcatccgga acgaccgttg
    17401 accaattaaa tgccgtctat ttggataatt tgcatgatca attcggagaa gttgtcagcg
    17461 tcagcgatga attcaaatgg gaatgggtct ccatccctca tatcttcgcc actccttttt
    17521 atgtctatgc atacactttc ggtcaattgc tggttctcag tctctataaa aaatataaag
    17581 aagaaggaaa aaactttatt ccgcaatata aacagatttt atcggccgga ggatcaaaat
    17641 cacccgaaga tatccttgcg gatgcgggtt ttaatttcta tgatccggca ttttggcagg
    17701 gaggttttga ttttctcacg gaaaaaatga tggaaatcga aaggaattga taaatcacct
    17761 gtaaaagaat aaaatattga tatgattctc aacaaagaac gcttgttaac aatgacgcaa
    17821 acatttttgt cgaaacgatt gcattccgat cattcgattg tttgtgttta tttaaccggt
    17881 tccatgctcc gggatgaccc tttcatcaac ggaaccgcgg atgtggactt ggtaattgtc
    17941 cacaaccaac ctgtggatga aatcagagag atcatcggga tgacccccga agtcacactg
    18001 gatattcatc acatcgaaca atcttattac tctcctcctc ggaaagtccg gaaagatcca
    18061 tggatcggat cttccctctg ctttgatccg cttcttttat ataacaaagg tcattggttt
    18121 gaatttatgc aagcttctgt ggaagccggt ttcttttctc cggaatacgt gattcatcgt
    18181 tccggtttat tctcaaatga ggctcggctt ctttttaccg aattggaaaa tcaaagaaat
    18241 ctcggcagca gtatatacat ttcttcttat ttgaaaatca ttgaagatgg ctgcaatgcc
    18301 gttgcctgtc tcagtggttt accgcttacc gaccggaccc tgatgaagcg atttaatgag
    18361 gtcgccgaag caatcaaccg ggaagatttg gccccaatat tatatggtct gatattggga
    18421 caaagcagcc cgtttcccta ctatgattat ttcttcaatt cctggaaata ctatttggaa
    18481 tacttcggga acagtgatct tgctccttta aaaatgaaag cagatcgtat tccatactat
    18541 acggcacccg tttcttatta ttggaacgaa catttggcat cggcattatg gattatggca
    18601 aaaacgtggt gcagtgtggc caaagcatac cagctggatg acaacgaata tttcggcact
    18661 ttttgtaata tgctggaaat ttctccgatg ttttttgcaa agaggaaaac ccaacttgaa
    18721 ttatttctgg attcgattga ggatgctcaa aagaattggg gtgattcgca agggcttgac
    18781 gcaaacgctc ttgaaaacct ataataaata tccacagatt tttgtggata ttttttaata
    18841 gacagtagaa ggcgttgata gaaaattgct gattacttct ttaaccactt attcatttta
    18901 tttacaatct gttcacacac ccataaacac aacttttttt ccgaatatcc gacaaatatt
    18961 ggcttttttc cttctcatcc acagtttcca ctccccttat taagaagaat atatattaat
    19021 taaaaacaac tgaagctatt taaacttcag ttgtttttaa ttaaatactg tttttattgt
    19081 ttggaaggtg cacctacggg acaaacttca gcacagttcc cgcattcctc acacaacttc
    19141 ggatcgatta ccatatagcc atctccttcg gaaatagcat ttgccgggca ttctgattcg
    19201 catgcaccgc aatcaataca aacatctgga tcaattttat aggccatgaa tatttactcc
    19261 ttttttttgg attacagttt tctagcatat cacaaaaata gggatatgac aatgtaataa
    19321 ataataatac cgatttatat aataaacgaa tagattaata tcataaaacg tagaacttta
    19381 tgaacagaaa agaatttaca cacaaatatt atttatataa aatatagaag ataatagaaa
    19441 acattcattt tgatattaga aaaaatagaa ttatgaaaaa tttgatcatt tgtatggaat
    19501 tataatatct caatttcaaa gtttttattc atcgataacc cactttgata catagtgctt
    19561 caattaattt ttttattcaa aagatagaat tggtcatttc atcgggaaga gacgattttt
    19621 tcgaggatta gcgaatataa aagaaattaa attccaaatt ccggacaaaa ccggtaaagc
    19681 aatgggttta ctattaagcg cttctcggaa aagagctata tgtctatttc ggaatagggg
    19741 aatattcatt ttaatcgtgt ggttctccta aaatcctaaa acagtccggg aaaacacgat
    19801 taaatgtttc acgtgaaaca tttctgttat acttttggag gagaaatgaa tgcctcttat
    19861 atccgctcaa aaattgtcaa aatctttcgg cgctgaagat attttttcag aaataaccct
    19921 ttccattccg agaggtgcga aaattggcct tgtcggaccg aacggttccg gtaaatcaac
    19981 tttattgcaa atattaatgg ggattactcc ctctgattcc ggaactgtcc aacgggcaaa
    20041 aaaaataacg atcggttatt tgccacaaaa ttttgaggct gaattcgaga aatcaccgta
    20101 tcaggaatgt ttgtccgttt tttctgattt aataatgatg aaaagcaaac tgaaccaaat
    20161 ggaacaggat ttagcaaacg ggaataatgt ttcggagcta ttatcaagtt atggtaatct
    20221 tcaaaatact ttcgaaagcc gtggcggata cgttttcgaa acaagaatcc atcaggtttt
    20281 acaagggctc ggtttttcaa aaagtgaaga aaatcgccct tggtatcagc tttccggagg
    20341 ccaaaaaaca cgggcatatt tggcaaaaat attattaatg aatcccgatc ttttgatttt
    20401 agatgagccg accaaccatt tggatattaa tgccatagag tggctcgaat cttttctaaa
    20461 agaatataaa ggaggagcct taattgtttc ccatgaccgg tacttcttaa atcaaacagt
    20521 ggaagtcatt tgggaattgg attcttcttt tgaaatctat catgggaatt atgatgccta
    20581 tctgcaacaa aggcaggctc gatatgaacg ccaaaaagct gaatttgaaa gtcaacaaga
    20641 atttattgca aaagaggaag aatatattcg ccgaaacatc gaaggccaaa atacaaggca
    20701 ggcacaagga cggcgaacac ggttggaaag attgctggaa gaatcgaaaa taacgaatcc
    20761 gatcttgcat cggaaaattc aaacaaatct ttcgtcaagt atccgatccg gagatattgt
    20821 tctcagaaca aaaaatcttg aaatcggtta tttggatgat cataaaaaat tgatatccat
    20881 tcccgatgtg gtgttgcaac gcggtgagtg tgtcgccatt ctcggtccga acggtgtggg
    20941 aaaaagtact tttatcaaaa ccatcttaaa tgagattcct ccgttagcgg gtgaagtgga
    21001 aatggggtct aatttaaaag tgggatattt tgctcaggct catgagaaat taaatccgaa
    21061 agcgacgctt atggacgaaa taaccgcagc ggctccgcaa atgttgcctg ccgagatccg
    21121 atcttatctg gctcgatttt tatttaccgg tgatgatgtt tttaaaacaa ccgagacgtt
    21181 atccggcggt gagcgcggtc gactcgcttt ggcaatttta gctcttcaaa atgcgaattt
    21241 gttgttgctt gacgagccga tgaatcactt ggatctcgaa gctcaggaga cgttacaagc
    21301 attattaaag gattttaatg ggacgattct tctcgtttcc catgatcgat acttagtaga
    21361 tgctatcgcg actcaaattt gggaagtgaa ccccgataaa aaaacgttgg aaatatttcg
    21421 cggaaattat tcggaatata aggaatatca aaagaaatcc gggatcagcg aattaaaaga
    21481 tcaaattgat tcaacggaaa ggaaatctcc tgagataaga gaaaatcctc ccttgcaaaa
    21541 gttgtcaaat aatgagatat ttagaaggca gaaaaaaaag gaagaactgg aaaaaaggat
    21601 ttcggtcttg gaaaaaatgc tcaacgaaat ttctcaacaa atggtttctc aaaaagaaga
    21661 tcatcaaaaa atcgcagagc tgggaattcg gtataattcc atagaaattg aattgaattc
    21721 cgtcatggag gagtgggctg aaatcgattg attaaagtcg attcaaaaag gaaaaaatat
    21781 gtacttatct gtgattgctc cggtttataa cgaacatgac aatattcctt tattgtatga
    21841 agaaatggtg aaggcattaa aggattttga taaagaatgg gaattcatcc tggttgatga
    21901 cggttccgta gacggcagtt ttgaggaatt aacaaaggtg gctgaaaaag atcccgcaca
    21961 tgtaaaagtg atcggtttgc gcaggaattt tggacagaca gctgccatcg ccgcaggtgt
    22021 tgattattca accggagaga taattatctt gattgacgcc gatttacaaa atgatcctgc
    22081 cgatattccg atgatgctcg gaaaaatcga tgagggatat gatctggtca gcggctggcg
    22141 aaaaagccgc caggataatt tatccaggaa aattccttcc gtcatcgcca ataaaatcat
    22201 cggtcatgta accggagttc ctcttcatga ctatggttgc actttgaaag cctatcgacg
    22261 cgaagtgatt accggattcc gattgtacgg agaaatgcat cgttttattc cggcttatgc
    22321 caatacggtc ggggcaagaa ttatcgaagt cgaagtcaaa catcatccgc gccggttcgg
    22381 gaaaccgaaa tacggattgg aacgaacagc aaaagtaatt ctggacttat ttacggttaa
    22441 gtttttatct tcttattccg ataagccgat ttatctgttc gggggatcgg gattattctt
    22501 aacgatcatc ggcgggttgt tgctgctgtt tttggctgtc cgtcgattgg gctggggcgt
    22561 ttccgtactg gcatctcctc tgttcacttt cagtatcatg atttttattt taggctttca
    22621 attgattatg atgggattat tggctgaaat gctcacccga tcatattatg aatcgcaagg
    22681 aaaacgcact tatacggtta aaaagttgat taatttcaaa tccgataaat gaaaatctta
    22741 accgttattc atgaatttcc accgatcggt ggaggcgggg ggttgattgc ccgcgatctg
    22801 gcttgtgcat tggttcaaga cggaaatgag gttcgggtta tcacagctca gttcggagat
    22861 ttacccgatc gggaagtcct ggacggcgtc gaaattatcc gattaaaatc tcatcgtaag
    22921 gaagcatacc gggcaaaact gctgccgatg gcttctttta ttttagctgc ttcccgttat
    22981 tgtgtttatc aaatgaagga tttttcgccg gatattatcc atgcgcattt tgcagtaccc
    23041 ggcggcccgc cggcaaagtt tatatcgggg aaaaaacata ttccctacgt tttaacggtt
    23101 catttgggag atattcccga cgcatctccc gaaaaaaccg gtaaatggtt taaattcatt
    23161 tatcctttta cacatacctt ttggaagcat gcttccgcaa tcgtagcggt cagtgagttc
    23221 tcgaaaagaa tggcacagcg aaaatatccg gtggaaatac aggtcattcc gaacggaatt
    23281 gactatgatc aagttcgaat cgaccgaatt gaacctcatt cggttcccga gattgtgttt
    23341 gccggaagat tcgttcccca gaaaaatttg gaacagctgg ttaaagtctg cgccgcgata
    23401 aaagatttga attggcattg tacattgatc ggcgacggac aagatcgcga aaaaatcgaa
    23461 gagctgatcg aacgattgaa tttacagaac aaaatttcta taacgggatg ggtagcaccc
    23521 gagcgcgtta cggaaatatt taaacaaagc gatatcctgt ttcttccctc ccgatcggaa
    23581 ggcttacccg tttccggaat acaggctatg acctgcggat tggctttggt attaagtaac
    23641 gccggcggaa atccggaaat tatagaagaa gggaaaaacg gtttcagttt tgatcccgag
    23701 gataccgatt cttttgcttc ggcgttacga gcgctgttgg aaagtccgga aatgctcctg
    23761 agctttcggc aaaatagcct ggaatcggcc aaaaaatatg atattaaacg gattgccaaa
    23821 caatatgagg cggtgtttga acaggttttg gctgctaaat aaagttcagt aacatttatt
    23881 tacttccgga caaaccggct cggggatcgg acaggctatt tctttcatcg gtgagcgtaa
    23941 gtagtgaaat tcttttccgt ccggcgtctg cccgatcaca gcgatccctt tgatatagga
    24001 actgccgggt tccaaacagc caatgactgc gacggaagat tgatccggaa aagtatcggg
    24061 gaacttatcc agcggaagga cgatatccga ttctccgtta tcgaaaagaa ttttgaagat
    24121 gatccgcgaa tagagctgat atgcgtacgc tttatccgtt ccatgttccc cttgattgaa
    24181 ataataaaag cgcggataaa gtgcaactcc ctgaaaaagg cgcatctttc cctcccgtaa
    24241 ctgtgtctcc gcttttgtcc aatcttgatc cggaaaagtc tctttcattt tttccgtgac
    24301 aatcagctcg ttctcggaag gaatcgaagg agaagtattc ttttcgatta agatcatgga
    24361 aaaatatgcc atgaaaaaaa tgccggtaat caaaactaat tttccgaaat ccccaattcc
    24421 gattgtttga tttgaaattt cggtacttcc ggttacgaga tccgtccgga tcggcgtgat
    24481 ttttttcagg attaccgcga gaccgattga gtagattaac agaacgcccc agttcatcgg
    24541 aacgatgtaa cgaccgccgg aagtacgggc aagccccaag ccggccgcat acacgatcaa
    24601 caaataagcc gccgaaagtc ctgcccagct ctcctttttc caaagcaaat tcaaaccgat
    24661 actaatcagg atcagattga ttatcagaaa aagtatctgc cctgccgaaa gactcccgtt
    24721 ccaatgatct ctccaaacag aatcttcggc tgtatataaa tgattcaaat catcgaattg
    24781 aatttggacg ggaaggataa aagcgatcga aaaaagatta tggaagaaat gatttccgat
    24841 tgtatcgatc aatggatgaa ccttcaaacg tatttcatcg ggaatcaatg aaaacgattt
    24901 taattgagtc tccgtctctt tttcaatttg cttgccggtg gcaggggtag cttccgtatg
    24961 ctgttgaaga acaggttcgg cggtcgcttg gacaggctct ttattctcga ccgaaatttc
    25021 ggaggtttcg gtgatgggtt tcagtctttg ttcgacaacc acggatttaa aagagtccaa
    25081 aatctcgggc gcaatcgttc cggtgatatg ataggatcga acgacccaag ggctgataca
    25141 gatcacaaag gacagcagaa agatcagaag gtcgcgtatc ccccgtttta tttttttatg
    25201 gaagacgatg cctccgaaca gaataattgc cggaatcatg gccacaaaat taaagcgggt
    25261 ataaatcagg agccccaaga ctcctccggc tgccgcagcg aagtaggttt gttttcttcc
    25321 ctgtcggaac catttgaaaa taaagtatgc aaacgctgcg aataatactt cggctaaaaa
    25381 ttcactcatt tccaaacggg aatgaatcgt cagaatctga gtggtgtgga gcgcattcca
    25441 ttcctgaaaa atcatcagag ctgctgccaa aaacccgctg aaaagccctc ccaaatcgga
    25501 agcaaataaa aatagaattg cgggaagaac ggcaaaaatc gcactttgaa tcagtaaagt
    25561 gagattcatt tgattccccg agagtaaggt acacaggtaa attacaaacg ttacaaacgg
    25621 tttcaacaca agcgtgccgt gataaaactt taagccatag acggcggttt gagccgccac
    25681 atcattcagt aatgcatccg aatacgggta atattcgtaa ttgggcggat aaggtcccgg
    25741 tgcaaagaaa tgagcttgca tcggcgccat gctccaagtg aaggcggcga taccccatat
    25801 caagaaaaaa aggaaagcac gattccgaaa taatttcacc cgtccgattt ttgtcatgaa
    25861 cggagaaaga agcaataaaa aaccgattat taagacaatc tgaagggatt cgacagggac
    25921 cgcatttccg taccaagctt ccttcccggc aacgattccc catcccgatc cgtaaataaa
    25981 caaaatggtc aacagaaaaa ggccgagaaa aataaaaaag ggcttccccg taatttgccg
    26041 gcaatcgtcc ttaattcttt tgaatatgtt gctgtccgaa aagacaatca agatcagagc
    26101 ggcggaaaaa ccgacccaaa acagtagaaa tagaaaagga cgcaggcgat gaaagtaggc
    26161 ggaaaattgt ccgaaccgat aatccggcat taacagcaag ccacccgaaa gaaccgccgt
    26221 aacggaagcg attcgtttga tctgctcctt gcaagcgaga atagacgctt ccaaggatgc
    26281 atttttttgt tgatttatgt tgaaaatgat tgcaccgatt aaggatgcca gagcaatgag
    26341 aaggaaaaat gccaacatga caactctttc gagagaaagt ccgaggataa aggcattttt
    26401 ttgatccgaa ggaattttaa atagagtata gccgcatatc aagctgacga cagtcagcag
    26461 ggaaaaaaac aaagggatgg gttttaatcc ccgtttttct tgagacggat cgattcccac
    26521 atgttctcac tttcaaaaaa gtaaatcaag aatataccgg ccaaaacgac ggcaaagaaa
    26581 atcagattcg cggttaacgg cgggcgtgaa atgatgaaga aagcaagtat ttcaagggct
    26641 gtcgcagcca aaagcattaa attgactgcc tgaacatcgg aaaaacccat ttttacgagc
    26701 cggtgatagg tatgatctgt cccgctgtca taaaaatgca aatgcctgcg gaagcgggaa
    26761 ataattacca ataccgtgtc aaaaatcggc acgcctaaca gaagaattgg cataaaccat
    26821 gtggaagtct gtaagcgatc cggcggatta taaatgatgg cgattgccgc aatcagaaaa
    26881 ccgattgtct gcgctccgga atcgccgaaa aacaggatgg caggcgagga attgaaaaaa
    26941 ttcagcacaa tgcaggctcc caacatggcg gaacataaca gagcaagcga ccattgtttc
    27001 gtatccatgg cagccaacac aaaaaagata agcgccagca aagagagccc taccatcaac
    27061 ccatccatac tgtcgacaag gttaaaagca ttggtaataa aaaccatcca gaaaatcgta
    27121 attccaaaat ccagaagatc atttaccgtt gcagaaaaac ccaatatttc acccacggtt
    27181 tgcattatct gcacttgaat cccgcagaaa tagagcacga tcgcggccaa aatctgaccg
    27241 agcattttcc atcgccaacg cagaggcctg taatcatccc acagtgcgaa tccaaaaatg
    27301 attgtggcac aaaggagcac ttttccggtt tggctgtcca aacggtgatt caggaagaca
    27361 gcggtgagaa tcagtgtcgc aaaaaaagcc aatccgccgc tgatcggaac aggtcttttg
    27421 tgaatcttat ggggttccga taaggggaaa tcgattaaat tcgttttatg cgagaaacga
    27481 attgcaatcg gaacgataat cagacataaa aagaaactga caaaaactgt ttcaaatatt
    27541 tttccggtag aaatctctgg cagaatcatg agtcttgtgt caattgttga tagattttga
    27601 aaatttcgtc gttcactcga gagatcgaga aaaactcaat tgctcttttt cttgccgcgg
    27661 tccccatttc gattcgcatt tcattacttc gaatcaattt ctccaaagca taagccaatg
    27721 atttcggatc ttttgcggga accagaaatc cggtgacgcc gtctacgaca acttcccggc
    27781 atccggggac attagtggtg acaatgggac gtccgcaaga agccgcctca attaatattc
    27841 tgggcaatcc ttctcgataa gatggcagac atacaatgtc cgatatggaa ataacggtca
    27901 gaatatcttc ctgccatccc caccattcta catttccttc tttttgccaa aaggacagct
    27961 gttcataaga tatgctgctg ggattgccgt catctttttt cccgaccaga gcgaatcgtg
    28021 cttgaagtga tttgctcttt aatatttcgg cggctttgac aaattctcct actcccttat
    28081 cccagagcat tctggcgggt aaaatgatga gaggaatgga gtccggtggt gggatcggac
    28141 gaaacaactc ggtatcgata cctgttccca aaataatatg ggtgtttccc tccgtcgtaa
    28201 atccgtattt ttcgaagaga gcggcatcat ccgggttttc aaaaatgacc tcggtatctt
    28261 ttaacgcctt cttatacaaa gaggacacga ttttccggat catcttggtg gtaattttgt
    28321 tatctaaaaa gatatagccc aagccggtga tggaattgac aatcgctcga atcccgacct
    28381 ctttggcggc aagagatccg tatataacac atttaatcgt aaaatgatga accagatccg
    28441 gcttttcttc cgcgtaaatt ttctttattc gctgaatggt tcgctgttcg ataaacggat
    28501 tcatcccttt ccgtgagaat tcaaaaggaa taaatcgaaa accggctgct gtcagttttt
    28561 cggtatgctc ccccgccggt gccattaata cgacttccca ccctttttca cggagatatt
    28621 tcgcaaaatc cagtcgaaaa ttatagagat accagtctgt attggcgaaa aaaataactt
    28681 tcaagggttt gatcctttta tttaatctga actaattata aagcagaggg attgatcttt
    28741 acgagccggc taaaagagcg aaagctgaac gccgtctgtc gcttcttttt tctcggctgc
    28801 ttcagaggcc ggattggcat tttcaaccgg ttccgctgta attttctctt ccacttcatg
    28861 aatataggtc ggtaaaaggg caaaatcatg cataatactg tcccgtaaat tcggttgatg
    28921 caatgcagct gccggatgaa acatcggaac gatcatccgc ccgtccaccc aatggccctt
    28981 cccatggatt gaactgattc tcgcaagcgg aaaatagcgc cccatcgaaa acctccctaa
    29041 agtaacgatg accaacgggt caatcagccg aatttgttca tccaaaaagg aagagcaggc
    29101 ggtcaattct tcgggcagcg gatcacgatt agccgggggt ctgcatttaa caacgtttgt
    29161 gataaaaaca tcctgtcgtt tcagtttggc cgtcgaaagc agttcgttga gaaaattacc
    29221 ggcttggccg acaaaaggtc tgccggtttc attttcattc aaaccggggc cctccccgat
    29281 gaaaaggatt ttggaatgaa tattcccttc tccggggacg gatttttttc tggataaatg
    29341 aagttgacac ttttcgcaaa cggcgatttg ttgtgcaagc tgagtcattt tttcgtagga
    29401 atcatccata acttctccaa tgtcaggata taagtttgat aatcttgcat ttccagattc
    29461 atcatcagag catcttcttg attgtcctga tagtaatgtc ttcgaatatt gacgacccga
    29521 taaccgatct tttcatataa ggatattgcg gctgaattac cggctcggac ttcaaggtaa
    29581 gagatccttg ccccttcttt ccaaccttca tacaatccgt tttgcattaa tgcatcgccg
    29641 attccccggc ggcggtaatc gggatgaacg gcaaaattcg cgacatgcag ctcgtccatt
    29701 ataagccaaa ggacaagaaa tccgcagaca cgttgctgag agtcgaccgc tacccagggg
    29761 cgtgatgcct cgttcctttc gacttcaaaa taataagaac tttgcggcca atacagagca
    29821 gccgaagctt ggtcgattgt gaatactgcc gggatatccg ttaaaaccat tctgcgaatg
    29881 gaaatgtcag tgcgcccgat catatttatt gcgaaatcgg ctctttcgta tgaagataga
    29941 tcggagacag ggtgtttgga ggaggataat ctcctttttc aagacgagcc caagcaattt
    30001 cggctaaaaa acccgccctt cgtaaacaca ttgccggtga tgccattcga atattcttat
    30061 tgtttctgcg aaaataggta cgcatatctg cggtcaattc tccgcagacc gttgtcggtt
    30121 ccgtaatcgc ttcgcacaga ctttctgtcg tatagacgga aaccggtaaa ctttccgccc
    30181 atttatcttt cgaaggattg taaaaagctg cagcaagctt tgttctgcct gcctgtaaga
    30241 cagcacagag cggcaatccg tttatcggct gagcggctgc gagaatatcc aaagtcggga
    30301 caccgatgac cggaatatcc aaccccagcg ccattccttt tgcaaatgaa aggccgacac
    30361 gcaggctggt gaacgatccg ggacccattg caaccgctat gccgcggagt tttgagatat
    30421 ccgtatcaac cgtatcgaaa agatttttga cggaagcggc aagaatgacg gtatgccgat
    30481 tcagactcag ccaatttgcc tctccgatga tactgtttcc gtcataaacg gcgattccga
    30541 tattactggt agaagtgtca attgctaata acatatttat ttaaactcca aaagaccggt
    30601 ggcgaaatga atgcaataag gtttgataac gttccccgta tggaaccatg accaggctgc
    30661 gttgttcatc ggccacccaa gccattttga ttgtcaatct ttctttcgga agaacggaat
    30721 caattctttc cgcccattcg acgactaaca cgccttgatg cagcatgcgg tcgatatcga
    30781 gctcttccgc ttcaagggta ctgtcgatac gatatgcgtc catatgaaaa agtgtgcttc
    30841 cgtccattcg gtaatactca tttaccagga caaaagtcgg gcttgttacc tgatcgacac
    30901 tgccccatcc ctgtgcgatt ccttggataa aagtggtttt ccccgatccc aaatctccgc
    30961 tgaagcacaa aacgtccccg ttttgtaaca ttcttcccag acgcatccca aaccgccggg
    31021 tttggtcggc gcttctgctg aaaatttcca atgtttccga atcaagaata ggcacaaacg
    31081 gatcctttaa gatatcaggt tgggaagaaa agtatcagat ttgctcgaat ttgttttatg
    31141 ttgaaggttc cgaagactca gatggacgtt cgggattctt ttgcggtttc cccttcattt
    31201 tatgcgataa ttctcttctg gtcagcaaga cgcgatgatg acaatttaaa cattccagcc
    31261 caatctcggc gccaagccga accactaccc actgattgga accgcatgga tgcatttttt
    31321 ttaatgtgat caaatcgcca agctcgtact cctcatccat cctgcaatta taaaggaatt
    31381 gaaaggggct gaacagagtc acaataacgg ttcgggatgc aaatgttata atagctgcat
    31441 ggccgtgccc gcagtttttg tatgtcgcat cctgacctta atcatcgcat tcacaatcca
    31501 tgaatttgcg catgggtttt tggctgatcg gttcggagac ccgactccgc ggctgcaggg
    31561 tcggttaact ttgaatcctt tgaagcattt ggatattttc ggctctttga tgttgttatt
    31621 aaccggtttc gggtgggcaa aaccggttca aatcgatacg gaagccattc gcagaaacaa
    31681 taaagccggt gtgatgttgg tctcttttat cggtcctttt tccaattttc tgttggcttt
    31741 attcggtgcg ttgctgttga aaattcccgg tattacaacc atccggtggt ggggaattcc
    31801 tttctttctt tcacaattcg tctggattaa tctcagcctg ctgattttca atctggtgcc
    31861 tctcgctcct ttggacgggg aaaaagtggt cgaatatttc ataccggaca gtatgcttcc
    31921 tgcctggaag aatgtacagc aatacggaat gcaaatttta ttgatctgtt ttttgatatt
    31981 gccgtacctg aatatcgatg ttttcggaaa aatcttaagt ccgttgatca gcggactgta
    32041 ttactggttg gtcggaggat aaaactatga ttcaaatcgc catcatcggg ctgggagaaa
    32101 tcggcacgtc gttggggctc gctttaaagt atcagaagga tgaaatatat cgggtcggga
    32161 tggatgttca taaatatgcg acggataatg catcggagaa gggagcggtc gaccgagttt
    32221 cccgaaactt attggacgca gtaaaagaag cggatatcgt ttttttggca attcccgccc
    32281 atgaagtgga agacacactc gaattaatcg gtcatgagct taagaaagat gccgcggttt
    32341 tggatacctg cccgacaaaa tcggctgcga tcaactgggc gaaaaaatcg atgtcccaac
    32401 cggaccattt tttgggaata tatccatcga tcaatccggc ttacctctcc gaaccgtcga
    32461 atgaatttca ttccgcgcat gaagatctgt ttcaatccgg tacattgtat ttggctccgt
    32521 ctgccgatac cgaacccgcc gtagtcaaac tggcgtcgga tttgggaacg ttgatcggtg
    32581 cccacagcgc atttatagat ccggctgaat tggatggatt attggctgct gcttatgatc
    32641 ttccgctgtt attatcgaac gctttaatga gcacgttgct caatcaaaac agttgggaag
    32701 attgccgcaa attagccgtg aaagaatttg tacaagagac cgatttttgt gctttccctt
    32761 ataccggaaa ggaattcacc gaaattctgt cggaaaatcg agaaaatacg ctgcgaatcc
    32821 tgaatgattt tatcgtaaat ttgaaaacct atcgggatgc cattcaaaac cgcgaccgtg
    32881 cggctttgag ctctctttat gaaaaagcgg aaaaaggaag ggaaaaatgg ctcaaagatt
    32941 acgaggaagg cgcttggcgg aaagaaaaag atcagaagct gaatgtaccg accgcggggg
    33001 aaatgatggg acagatgttt ctgggcggat tggggcggaa aaagaaagaa taaccgctga
    33061 tgcgggtaac gggcaaacaa cattgtcggc gtccgggacc ggtctgtttt attgttatat
    33121 ggtggaatgt tccaacggat ctttttatac cggttggacg acgaatcccg aaagacggct
    33181 taaagctcat aattccgggc acggtgcgtc ttataccaaa atgcattccc ccgtatccct
    33241 cgtttatgtc gaaaaaatgg aaagcaggca cgaagcaatg attcgcgaga cggaaatcaa
    33301 aaagtactct catgccgaaa aagcggcgat gagcgaatct tggaaaaaat caactgtttc
    33361 cgaagaacgg gaccggaaag atcagcaggg taaataattg gaaaatattc agacttttcg
    33421 ttccggtttc gtcagtgtca tcggtaagcc caatgtggga aaatcaactc tgattaatta
    33481 tcttttggga caaaaaattg ctgcggtatc tccgcgaccg caaacgactc agcgcaacca
    33541 gttgggaatt atcaccgaaa aagattatca gattattttt atcgatactc ccggcattca
    33601 tcagccgaaa tcaaaactcg gtgattttat gaacgaagcg gccgtgggga catttaccga
    33661 agtggatatt attctctgga ttgtcaacct gaatgaaccg cctgctgcgg aagatcaaat
    33721 cgtagcggaa aaaataaaga atgccggaat cctttccaat accgtattgg cgttgaacaa
    33781 agcggatctg gtcagtccgg cgaattcgga aagatatgcc gatcgttacc gcgcgttggt
    33841 accgggggcc gaatcctttt tgatttccgc caagacaggc gcgggatgtg atgatttgtt
    33901 ggctgcgttg gttcgcaggc ttccgcccgg gccggctttt tatgacgaag aacagattac
    33961 ggacttatac gaacgggaaa ttgcgagaga tttaattcgg gaatctctgc tgaagaatct
    34021 tgaagatgag atccctcatt cgattgcggt tcggattgat gagtatgcgg atcgcaccga
    34081 gaccaagtct tacatccaag cgacattaat attggaccga gaaaatcata aaagtatcgt
    34141 catcggaaag aacggggaaa tgatcaaaaa aatcgggcaa gatgctcgct cggaaatcga
    34201 aaaactgacc ggacgcagta tttatcttga attaaaagca aaagtggtga agaactggag
    34261 gaataatcct tcgatattaa agtcattggg ctatttttta ccgaaagaat gaaacggccg
    34321 gttcaatcct ctaaatattt atacttttga aatccctcac ccatcacttc gtttgcttga
    34381 ccgacaacca tgaaagcttt cggatccact tcgaccacca gccgcttcag ctgattgatc
    34441 tctccccgat taataacgca atagataaca ccctttttct ctttcgaata acccccgatt
    34501 cccgtaatca gggtaacact atgttccagt acattgatta caccttcggc aatcgcatcc
    34561 ggcttgtctg tgataataac cacgtcccga aaaaaattgg caccttcgga aatggtctcc
    34621 gcagccaccc cgctgacata gatcgcgatc agcccgtaca gtgccagatc ccagccaaaa
    34681 gtgagcccgc ccagaataac ggatacggaa tcggtcaata aataagattg tgtgatcggc
    34741 aatcccgctc tttggttcaa aattcgaccg atgatatcgg ttcctccgct ggtgccgctg
    34801 ccgcgataga ccagtccgaa tccaatcccc aagatgacgg caccgaacag ggtgttcaga
    34861 aaaatgtcat gggttaaata ggcatttttc tgatatcggg tcagcaaatc ggtaaaaaat
    34921 gagaaaagta caaccgaaaa aaccgtacga atcgcaaatt tccgcttccc caaataacgc
    34981 catccaaaga taaaaagagg aatatttccg atcagcgtca taccaccaat cggccaaccg
    35041 gtgtgagcgt tgataacctg tgccaatccg gagactccgc cgctgattaa tcgtgccgga
    35101 atcaaaaaat acgccatccc gattgattgg ataacactcc cgatcaaaat cagagaataa
    35161 tcggaaaatg tttctttgtt aaaaaaatct ttcttggaga cggaaaggtt ctgcttcata
    35221 ctttgattaa atcatagaaa ccttaaaaat tgaaggaaat acgaaggaat aacaaaagga
    35281 aaagtcgaag atcacctatc aaatattctc ggatccgtca cttgcgtcat agttttaata
    35341 aagatttaat tattctattt tttaatgtgt cataaaatga gtgcctaaga gaaaaattga
    35401 ttgagaataa tgcgaattta ttttcgaaaa ccgcaacgtt tctttcaatt aaccgtagat
    35461 taatatgttg cgtatagatt atgaacaaaa agctgtaact aagagaaaaa aatgaattgt
    35521 agtaaaaaaa agaaatacat tcttccccta ttcatcctcc tttttattgt aggttgcaat
    35581 cgttctcagc aggcagctcc gaccgccgaa attccaagcg aagcggtcac acgtgggacc
    35641 gtacacaatt ccctgatttt agtgggaaat gtggaagctt ctcaatcttc tgaattgagt
    35701 tggaaaactt ccggcgtagt tgattcggta tcggtaaagg tgggggataa agttgaatcc
    35761 ggacaggttc tggcaacctt attgactgac tcgatgccgg cttccagtat agcggcggag
    35821 gcggataaga tcagcgctga ggataatttg gaggaattat tggtatccga aacggcaaaa
    35881 gcgacggctt ataaaaatct tcgggatgcc gaagtagctt tacgcgatgc ggaaaaactg
    35941 gccgaagggt taaattatcc gattgcagat caaagtgaaa tcgatgccgc cgaaaaagcc
    36001 atgatcgctg cccgagatgc ctaccagaac gcggttgcgg attacgaagg tgtcaaatta
    36061 cgggactatt atgacgaaga gcggatgaag aaatatgaga ctcttcaaaa cgctttgaca
    36121 gcgtatgcca aggcttatga tttatggctt tattatgtca ataatgcgcc agaaataacg
    36181 aaaaaagtgg ctgccgcatc tgcgaaagag gctcaggcaa gttatgaagc cgcattaaag
    36241 gaatttaaaa catatcaagt ctcctttgtc cggaagcagg atcaggctga tgcggaagcg
    36301 accctggaag atgcggtcac ggcgtataac gcgcgatctt taatcgccga tttgacggga
    36361 acagtgaccg tagtgaatac ggctgcggga gattatgtca aaaagggaca aagcggcttg
    36421 cgaatcgacg atctgagtac cttatatatc tctttggata tttcggaagt ggatgtaaat
    36481 gacctgaaag acggtatgaa agcggaaatt acgctggatg ctcaaccgac gaaagtctat
    36541 caaggaacag tcagatatat ttccgctttt gcatccgaga acgattacaa cgtcacctat
    36601 cgtgcggtgg tagcttttga gcaaccggat gatttggtga aagtgggcat gacgggagaa
    36661 atcaagatca tcctttcaga gaaaccggat tgtttgcttg tcccgagctc tgcgattcgg
    36721 gaaggggata acggttccta tgttgatgtt gtggtgaacg ggacactgac gcctgtttcc
    36781 gttgttacgg gtgtggaaga aaacgggatg gttgaaattg tctccggaaa tctcaacgaa
    36841 ggagatttgg taaaagttcc ttcagcgtaa ttggggattc gaacagaaat tttaaatgaa
    36901 tgaagaagtg aaagattatt ctaaacaaac agtgaagaac aggaatgaat tatgagttca
    36961 acgggtaatc aaccggtcaa agggaaaaag aaaagaagaa aatcaacaat tgtgatttgg
    37021 gtattaattg gaatcatcgt tttgatgctg gctgccggcg gttatttata tcagcaacaa
    37081 aaacaggtcg ccgcggaaaa tgcatccaaa atttcgagtc aaatgactcg gaatgtcaaa
    37141 gtggaacaag ggttgatttc agagaatctg gaagaaatgt cgggtacggt tcgatcgaac
    37201 caatcgatca gtctgaattg gaaaatcagc gggacggtat cggatgttta tgttcaaccc
    37261 ggtgatcagg tgaaaaaggg cgacattctg gcggctttag atccggccac aatcgatact
    37321 tccatactgg atgccgctgt cacaaaagaa gatgcgcaag aacagttgga acgcctctat
    37381 acctcttcac tggatctcgc tacagcctat aatacaatga ttaccgctca agatgctgtg
    37441 aaaacggctg aagatgctct ttcggcattg ggtgtcgttc gtgaagatga aatagaaatc
    37501 ggcgtttact accaggatta tctgaacgca aaatccgctt atgaaagcgc gttggataac
    37561 tataatacgc tgaaagttcg acccttagat gatgtcgacc ggcaacgggc ttcccaaaga
    37621 ttggaaagtg cccgttcggc gatggagagt gcactttcca tgtataactg gtacaacggt
    37681 gaggtcgaca gcctggaaaa acaacaggcg gaagcggatg tgatcttgaa aaaagcgcaa
    37741 tatgatgacg cggtccgcgc ttacaacaag atcaaggacg gtccgacgga atcccaggtc
    37801 aaatctctgg aagcacaagt aaaatccgcg gaagcgacgg ttaacacagc ttatattatt
    37861 gccccgattg atgggacggt ggctgaagtg gcttccaagc aatttgacgt aattacattg
    37921 gacaatacaa cggatgaagt tttagccatc cgcctggaag atttatcctc ctattctatt
    37981 gatgtgtctg tttcggaaat ggatgtgaac tcggttgaag tagggcagac cgccgagatc
    38041 acctttgatg ccgttccttt gaaaatgttt accggaaaga tcgtcagcat ttccaatgtc
    38101 ggggatacga gcggagaaga agtaacttat gctcttacga ttcgaatgga tgataccgac
    38161 ccttcggttc gtccggggat gatggcggat attcaaattc gcacgaaaga agctgccggt
    38221 gctttgattg ttccgaagac agctgtcgga aaggcgcagg acggcagtca atatgtggaa
    38281 aaagtcaatc aggacggcac gacaacccgt atttcggtta caaccggaat ttcttcggac
    38341 gagaacattc agattctttc cgatcaaatc caagcaggtg atgaggtaag ggaaaccgtt
    38401 ttaccggata tgtcgcaaaa tgccggagca accggtgccg gcggactttt tagtgggatg
    38461 ccgatccgga ttggcggcgg tgctgccgga ggcggtgcgc ctcgcgaagg aggtcaaggc
    38521 ggaccgcctc ccgacggtgg cggcggacag aggccatgaa attgctgagg gaaaaatgac
    38581 agaaaaaaac ggaaaaaatg taattcatac ggaacattta aagaaatatt atgtcatggg
    38641 cgatatcgaa gtccgtgctt tgaatgatat ctcgttggat attgattatg gggagtttac
    38701 agccattatg ggaccatccg gctccggaaa atcaacttta atgaatattt tgggctgttt
    38761 ggatcgtccg acggacggga tttatttgct ggacgggatc gacgtatcgc aaatggatga
    38821 ttcggaactg gctgaaattc gtaaccgaaa agtcgggttt gttttccaaa gcttcaattt
    38881 gcttccgcgt tccacggcac ttgagaatgt gatgctgccg atcctctatt caaagaataa
    38941 agcaaatgcg aaagaacggg cggcggaagc cctggaattg gtggggctgg gcagccgaat
    39001 gaaacataaa ccgaaggagc tatccggcgg acagcaacaa cgggtggctg ttgccagggc
    39061 gttggtaaat gatccggaaa tcatcatggc ggatgaaccg acggggaacc tggacagtaa
    39121 atccggcggc gaaattatgg aattgttgat taaactgaat caggaacgcg gaaagaccat
    39181 catcatggtg acgcatgatc cgcgtatcgg gcggcatgtc ccgcgcgtga tcagtgtgtt
    39241 tgacggaatg ttgggaacgg aggaggaaca ggcggttatc cgcagctggg tgaaaccgga
    39301 tggggaagat attccggata ttaagggaaa atcctccgaa aagaaaaatg atgcaaacga
    39361 taaacagaca aacgggaaaa acggatcgaa cggcaacgga gaaaaacatc aatccgaaaa
    39421 tacggataaa aacgacccga ttgaaaataa gaatacgaat gacaacaagg cggatgaccc
    39481 gtcttcggat gatgatccgc cgaaaaatgc cggtcatata accgctaccg aagcattaat
    39541 cagagctttc gggaacggga aatcttctcc aaacggcggt gaccattccg acggcgacgg
    39601 tgatcatggg gatcataaag caggagaaac aaacggagct gcagatgctt tggcgaatgc
    39661 gttcgggaaa caaattgacg aagatgcgat tcgagcggca caatcttcat ccgtgaagaa
    39721 acaacctaaa gcgggaaagg gtggcgcgag atgaatctgg gaacattgat ccatgaggca
    39781 atcactaacc tgctttccaa taaagttcgc tcgttactga ctatgttggg cattttcatc
    39841 ggtgtcggtg ccgttatcgc gatgatgtcg gtaggattgg gcgcgaagaa atccattacc
    39901 gaccaaatga gcgccttggg agcggatacg ctttatattt ctccgggaaa ttttacccaa
    39961 accgttcgaa atccgggaac gcttacgaac gaagacgcaa acgcaatcgc tgcgttagac
    40021 tgcattgaca acatctctcc gacttcttcc gctcaattga cggtttcccg agcggggaaa
    40081 caatccagta ccttgaccgt taccggcgtt ttcccgactt acttgacgat cgaaaactac
    40141 agtttagccg agggcagatt tatcactcag gctgatttgg ataataatgc catggtgact
    40201 gttatcggtt ccgatacccg ggatctgatg tttgaaacct atgaagaggc ggtcggaaat
    40261 actgtccgaa tcggcggcca accctatgaa gtggtcggcg ttctgaaatc gaaaggggac
    40321 gcgagtatga tcagttcgga tcgcggggtt tacgttcctt tgacaaccgc tcagaatcgc
    40381 ctgataaccc ggcagaaaca tgccttggat cagattgcta tgaaaatcaa tccggcgtac
    40441 agtgcagaag ccgccgaagc tgccgtgacc gcactgatgc gggatcggca tgatatcctt
    40501 tccggcgaac cggatgattt ctcgatcttt aatatgcagg aaatcgtcga taccatgaat
    40561 tccgtcatga attcctttgt gattttcctc ggaagtgtcg ccggaatttc gttgttggta
    40621 ggtggaatcg gtattatgaa cattatgttg gttaccgtta cggagcggac gaaagaaatc
    40681 ggcttgcgaa aagcattggg tgcgaaaaat cgggatattc aacttcaatt tttggttgaa
    40741 tcttccgtac tgagtttgat cggcggaata ttcgggatca ttttcggtta tgtattggca
    40801 tatgccgttg cggcgattgc ggcaaatgcc ggatctccga tgacacccga aatcaacccc
    40861 ttgattatta tcggaacctg tctgttctcg atcctgaccg gactgttttt cggaatttat
    40921 ccggcgaatc gagccgcaaa actggaaccg gtgattgcgt tacgaacgga atgatgacgg
    40981 tgaaaaacat ggttgggaga aaaatggctc tgcttaccgg actgaaatca tccggtggct
    41041 tacaaccgaa aaaaatgctg cggaatttgg cactggtgct ctgtctgttt gccgttatag
    41101 gatgctcagc cgtttcccag aaggtcggta tcggatcgac ggcaactcag gcgggattgg
    41161 aaggaatacc ggtaacccgc ggggacatca gcgccgccgt tacgctggtg ggaaacgtga
    41221 cttatgctca atccgcggtt attttgtgga agacaaccgg tgtggtacaa aacgtgaatg
    41281 ttaagatcgg cgatcaagtg aaacaagatg atatcttggc tgaattatct gaggattcgt
    41341 tgaattcctc tgtgctgatc gccgaaaaaa atctgataga tgctcaggat aatctgacaa
    41401 atgtaatgga atcgacaacg gcaaaaagcg aagccttgtc cacgcttacg gctgcggaaa
    41461 tcacttacca agccacaaag caagcgcagg aggcgttata ttatccgcga ggatcgcaac
    41521 tggatgtcga aatggcttat gatgcgtatc agttggcttt ggaaaactac aactatgcaa
    41581 aggaagatta tcgggtggtg ctggcaaact ataaaggatg ggatgacgcg gccagaacca
    41641 cttactatca agcatataaa accgcttatg acaatttgat ttcaacctat gaggattgga
    41701 aatacctgag cgggacgccc gataatgttg agttggcatc cgcccaaggt gaggtgttga
    41761 gcgctcaaaa agcttttcag gatgcgttgg atacttacag ttcttatcag acacttccga
    41821 gagacgaaga tctgcacgag gccaacgtga cggttcattc ggcggaatcc agttttcagc
    41881 aacgatatgc gattgcgccc tttagcggaa ccgttacagc cgtttatgcg gagccgggtt
    41941 tgtatgtcac ttccggtacc tcggcttttc agctggatga tatgagtgag tattacattt
    42001 cgttgaatac ttcggaagtg gatgtcaagg acatttatgc gggacagaag gtgcaaatta
    42061 ctttggacgc gattccaaat accacattct cgggaattgt taaacaggtt gccggtgccg
    42121 gaacttcatc gactaccaat accgtgttta aaacgctgat taaatttgat caacaggatc
    42181 cgcaaatcaa agcgggtatg actgcggaag tgaatatttt cctcggaacg aaagaggatg
    42241 ttctgcaggt tcctttaagt gcgatcaatc aggagaacgg gcgttcttat gtcctgaaaa
    42301 taaatggtgc cgagacccaa aaaattgagg tcacaaccgg tgttacatcc ggtaacctga
    42361 ttgaagttaa ttcggatgag ctcaaagaag gggatcaggt tgttgtttcg gaaacggatt
    42421 tacagaatta tttgaattcg ctgacggaaa cgatgacgac tccactatct gcggagtcgt
    42481 cggaaggcac ctctttggca ccgatcggaa cttttcagcc ggtggggtcg ggtacgcctg
    42541 cggctccgat cggaactttt caaccgcaga aaaccggtat gaaccccggc cgcccggcag
    42601 gtactcggcc ggctttttcc gggacaccgt atccaccaaa tatgaacaaa acagaaaata
    42661 acggatgaaa aatggatcat aaaataacgg ttcaaacaaa aaatcgaaaa aatacctgga
    42721 tcattctcat tctgacactt gtcgtcattg ctctgggtgt atttctttac attttttatg
    42781 tgggtcaaaa agccagccag gctgcagtag cggctcggat cgccgccggt caacccagtg
    42841 tttcttcggt tgaatccggt ccgatgaaat tggtcatcga ggatatcagc ggaacggtac
    42901 gttccaatca atccgtagat cttgcctgga aaacaagcgg aaccgtcgca gcggtcaatg
    42961 tatccgtcgg tgattcggta aaacagggtg atatattggc ggagcttgac ccaaaaacgc
    43021 tttcgattca aatgcagaac gctcaggttg agttggatga cgccaaggat gatatggaaa
    43081 aattaaccaa taatgcggaa gcgaccacct cggccgtaac cgaattggta aaagcacaac
    43141 aggaattgga tgatgctcag aaagcgtatg acagcctgga tccttcccgc gcatggacgg
    43201 atgccgttca attggcatac gaaacctatt tgaaagctca aaatgattat gaatccgcat
    43261 tggcaaaatt tgaagtcact cgcgattatc cgatagatga tgccactcgt attaaaagat
    43321 tgggcgatgt cggcggctat cgcagtgtcc gcgataatgc gctggcggaa tatcgtcgct
    43381 atttgggaga aggaaatgaa atggagcggc tgattcggga agctgcgttg cagcttgcca
    43441 aagcgactct cgaagaaaaa caataccaat atgataaagt gaccaaaggc ccgaccgaag
    43501 ccgaaatcaa tgcggctcag gcaaaaatcg atgcggcaca gcagaaactc aattcttcca
    43561 aactgatcgc accgttcagc ggggtggtaa cacaattgga aactaaggtg aatgatgtca
    43621 tttcggaaac agccgcagct tccgcaacca aaacaaccgc aatccgaatt gaagatttgt
    43681 ccacttttta tattgatacg acagtcaatg aactggatgt aaattccatc caaccggggc
    43741 aggcggcgga gattcaattt attggaattc ccgataagac ttatcatggg acggtgacga
    43801 cggttgccga tacgggtgta tcttccggtc gatcggtcac ttttaatgtg acgctgaaaa
    43861 ttgatgatgc ggacgaactc gtgaagtccg gaatgaccgc cgatttatcg atgaccattg
    43921 cgaatatccc cgaaacgctt tatgtcccat tgtcttccgt actgttaaag aacggtcagc
    43981 cgtctgtaaa tctaaaaaag atggatggat cttttgaatg ggttcccatc gaaaccggct
    44041 tggtttccgg aaccaaagtg caagtattat cggataacct gaaagccggt gatgagattg
    44101 aggcggatgt gaatgtcgat tcgaccgata cgaacgataa tcggatgttc ccgatgggtt
    44161 ttggcggtag aatgtaattc ggatcatatc gggatttctt aaatacgacg atagttgatg
    44221 aaatgactgg ggtcggagcg tcactccggc tccggtcatt ttgtgacaac agataagtgg
    44281 taaaatgaaa tgaatttccg gcattactat ttctctccgg ggattttttt atatctgtca
    44341 acaaaaaagg gatgatgaac gaatgaaaat attgattgct tgtgatatgg aaggaattac
    44401 cggtgtgaca cgctgggagc attgcgatcc gacacatccg gaatatcagc gtttccgtaa
    44461 acaaatgacg atggatgtca atgcggctgt tgaaggcgct tttgccggcg gtgtcgagga
    44521 agtgcagatt gcggacggtc attcccaagg acagaatctg ttgatagaag aacttgattc
    44581 tcgtgcccaa ttgaattgcg ggactacctc cccgtggagc atgatgaacg gaatcgattc
    44641 cgcattcggc ggagtgtttt ttatcggata tcacgccatg gcagggacgg cgaatgccat
    44701 cctggcgcat acttggtcca ctcaaatcct taattggaaa atcaacggac agaagatcgg
    44761 tgaattcggg atgaacgcat tgattgccgg ttggttcgat gtaccggctt tgatggtcag
    44821 tggagatcag agtctggcta cggaagcttt gcggttcgtt ccggatatag actgtgctgt
    44881 tgtcaaagaa gccaacggtt atttttcggg gaaatgcttt tctccggcaa agactcatga
    44941 gcgcattctg gaatgtgccc aattggctgt gcaaaagaaa cgagagggac ggggacctca
    45001 accactgaag atgaatgctc catacgaagt ggaagtggaa ttcacccaac cctatatggc
    45061 tgagacagcc tcccgattga cgggatcgga acgtaccggc ggtcggtcgg taaccatcac
    45121 agtgccggat attccggcag cctttttggc ggcaaggacc ttatgtaatc tcgcggtgta
    45181 acaatttaat actgcttcgt gaagggcgac cgaaaaagca gacctataaa aaggacttta
    45241 tatgaaatac ttttcggata cctcgaatcc cgaacaaaac aacaggaaaa cctaccaagc
    45301 attaattatc acgctggcgg gtaatttaat attggcttta tccaaacttt atgttgccca
    45361 tgtatccggc agctcggcac ttcgagcgga tgcttacaac tctctttccg acgtcttgta
    45421 ctccgtaaca ttggtgatcg ggatgatgat cgccattcgt ccggccgata tctctcatcc
    45481 acagggtcat cggcgtttcg aaccgttggt gggactgttg atcagctttt ccatggcatg
    45541 ggccggctat caggctattt ccgaatcaat cgataaaatt cgaagcggcc cgacgacttt
    45601 tgatttggga ttaccgatct tggtgctgtt tttctcggcg gtaatcaaag gagtgatgta
    45661 ttggttgatt catcggattg cgatcgatct ttccagcccg acattgaaga cagccgcaac
    45721 cgataacctg atggatacga tcacatcatt taccgccatt atcggaattt atctttcaag
    45781 catttggtcg ccattggctg acccgatagc gggtatcctg gtgtccgtat ggatctttcg
    45841 cgccgctttt gaagcccttc aggaaaattt gggctttttg accggagccg gtgcatccga
    45901 agaacgccgg cagcagtttt tggatattat ccgttcggta ccgggagtgg cgaatgttca
    45961 ccagctgttt gccgaacatg tcggatcaac ttttgtactg gacgtgcata tcaatgttga
    46021 cggaaacaca ccgctttatc aggttcatcg gatcgaaacc gaaatcgaag aaaagctgac
    46081 tgcgattccg gatgtggaac gagcttatat tcatgtcgaa ccgttgggat atgaatagcc
    46141 ccggactgta ataccggcaa cgatatttta ttcccgctct gaagaagcga tccgaatttg
    46201 aaagaaggat ccgaaacgca tcatttatgg acaggaagat cccgatcgat tgacttttat
    46261 caacaaaatt cttagaatct tatgataaaa ataattaggc ggaatttgaa aagatgaaag
    46321 cggttataca gcgtgtcagt cgggcttcgg tcaccgtcga tgggcggtta atcagccgta
    46381 tcgggaccgg tttgctggtt cttttaggag ttgaagaagg agatacccga gaaaatgcgc
    46441 ttgcgatggc aaagaaaact gccgcattgc gaatatttcg ggatgaaaac gataaaatga
    46501 acctttctgt tcgggattgc gccggggaag cgattgttgt ttcccaattc accttatgtg
    46561 cggatacacg gaaggggaat cgcccttctt tcatcggggc cgctgcaccg gaaatcgccg
    46621 ctccgctggt ggatctgttc gcggagtcga tgcgagaaca gggtgtcccg acacagcagg
    46681 gtcttttcgg ggcggatatg aaagtcgaac tcttaaatga cggaccggtg accatcctgc
    46741 tggaaaatta gttatggaaa tcagtccgaa aattcaggga attttagaga cgctgccgaa
    46801 taaacccggt tgttatctga tgaaaaacgc cgatgacaaa atcatttatg tcggtaaagc
    46861 gatcaatctg cgtcaccggg tccgttccta ttttcaaaac agtgtgaagc atacggtaaa
    46921 aacccgccag atggtcagtc atattgatca tatcgactgg attgtggtgg aatccgagct
    46981 ggaagcgctg attcttgaaa tgaatctgat taaaaagaat cggccgcaat acaatattca
    47041 gctcaaagac gataaacgct atccttatat taaaatcgaa tttcaaaatc cgtacccgcg
    47101 gattctggtt acccgccaaa tggttcagga tgggtcgcga tatttcggcc cgtataccag
    47161 tgtttgggcg gtgcatcaaa cattggattt gctgcggcgt atcttccctt atttgacctg
    47221 tgatcgggag atcaccggtc aggacagtcg accttgcctg tactatgata ttaaactttg
    47281 ttccggtcct tgtatcaatg caatctcgca agcggattat tatgcgatga tggaagatat
    47341 tgcctctttt ctggagggga aaaccgccga cattgtaaac cggctccaaa agcagatgag
    47401 cgatgccgcg gagaatttga actttgaacg ggctgccgcc ctgcgagatc aaattcaggc
    47461 tattgatcgg gtagtcgaaa agcagaaaat cgtgagtaat gatgataccg attcggatgt
    47521 gatagccatt gcgcgctcgg aaaaagaagc ctgtattcag ctgttctata ttcgaaacgg
    47581 gaaattgatc ggccaggaat atttcattat gaaagggacg gaggatgaag ccgatcagga
    47641 agtggtttcc gaatttatca agcaatttta cagcgatgcg gcgacgattc cgcctcaaat
    47701 gctgcttccc gttgagttgg aagaacatcg cattatccaa caatggctgc gtcaacaaag
    47761 aggaggcaaa aaatttacgc tcaaagtgcc caaaaccggt aacgaaaagg acctggtgaa
    47821 tatggctgcc gaaaatgcaa tcgaaacgct tcgggcgctc aaaacgcaat gggagaatga
    47881 taccaacaaa caaagcgaag cattgactga actgcaatcg gcactgcagc tgcccgaacc
    47941 gccgaatcga atcgaatgtt atgatatttc caacacacaa ggaacgttgt cggtcggatc
    48001 catggttgtt tttgagcagg gagttccgaa gaaaaacttg tatcgacgtt ttaatatcaa
    48061 aactgtcgaa ggaccgaacg attttgacag tatgacggaa gtacttaccc gccggatgaa
    48121 gcgttggcaa tccgcgagcg agaaggataa caaaacaggg ggcaaaatcg atccctcatt
    48181 ttctgttctg cccgatttga tcatcatcga cggcggaaaa ggccagctgg ggcgcgcggt
    48241 cgaagtgctc aaggatttcg atctgcttga tcgcgttcac gtcgtcggtc ttgccaaacg
    48301 cgaggaagaa atatttatgc cttttcattc cgatccgata atccttccgc gtaattccga
    48361 aggactctat ctggttcagc gcattcggga tgaagctcat cgttttgcca tcaccgctca
    48421 tcgcggaaga cgaacgaagg aggggctggc ttctcaactc gagaaaatcc cgggtatcgg
    48481 gccggttaaa cggaaagcgt tgctgaaaaa attcggcagt atcgatgcca tcgccgccgc
    48541 atccaaggaa gaactgatgt tgacggacgg tatcacttcc tctttggcgg atgcaattcg
    48601 cgattatttc ggatgaagaa cggtgactgc acttttttac gcctcgaatg aaagtataat
    48661 taattgatag caatattctc aattaaacaa gaaagggatt gatgatggaa aaaactgcgg
    48721 aacaaattcg ctatgataaa ttgggtgaaa cggttgccaa ggcgttgcgc aagaggttct
    48781 ttgatgcctg gtatttcgat aacaaagaag cagcagtggc tcaagcgctg agcctgattc
    48841 cggaagggac ttctgtctct tggggcggat cgatgtcaat cgtagaagtg ggtttaaagg
    48901 ataaaatcca aaacggaaaa tataaattga ttgatcgtga tacggcaaaa actcccgagg
    48961 aacgaattga gatcatgcga caggcgctgt tggccgatac ctttttgatg agtgccaacg
    49021 ctatcagtga agacggccaa ctgtttaatg tggatgggaa cggcaaccgt ctcgctgcat
    49081 tgctgtacgg accgaagcag gtcattgtca ttgccggcgt gaataaaatc gctcatacgg
    49141 ctgcggatgc gctggaacgg gctcggacga ttgcggcacc gacgaacatt tcccgctttg
    49201 acgtagcgac accgtgcaaa atcaccggta cttgttcgga ctgtacgacg gcggagagcc
    49261 agtgcgctca aattgtccaa acccgcatct gccggccggc cggaaggatc aaagtgattt
    49321 tggtcggtac caagctcgga tattaatcat caaggattcc aataatcgtc agagactgtc
    49381 agcaattttt gtgaaagtgt ttgacaatca ttgttcttta tgttctaata ggaagaatta
    49441 aaaatttata agcccctgaa tggcgctctt atccagagag gtggagggac cggcccaatg
    49501 aaacctcggc aacctgatta tcaaggtgcc aactccggca gagtgaatga aaaatctgga
    49561 agataggagg gtctgtgatg accaatcgca cccccctgga aactcggggg tgttttattg
    49621 gaggatatcg ttatgtcaac atttatgaat tccccgaagt acctttttac ttcggaatcc
    49681 gtcacagaag gccatccgga taaaatctgc gatcaaatca gtgatgcgat attggatgct
    49741 tgtctggaac aagacccgta ttcacgggtt gcctgtgaaa catccgtcaa aaccggtttt
    49801 gttctggttt acggagaaat caccacacag gcgtttgtca attttgatga gctggttcgc
    49861 aaagtggttc tcgacatcgg ctatgattcg agtgaaaaat gcttcgatgg gcatacttgt
    49921 gggattcttt ctgcgatttc cacacaatca ccggatattg cgctgggagt ggataaagcg
    49981 caggaagcga aacggggaga attcggagaa gtcgatttcg aagaggttgg cgccggcgat
    50041 caggggatga tgttcggtta tgcctgtaat gagacaccgg tttacatgcc tatgccgatt
    50101 tatctctccc atcgtctgac cgaaaaactc tccgaagtcc gtaaaaacgg gactttacct
    50161 tgggtctatc ccgacggcaa aagtcaggtg acgatcgaat attcctacgg aaagccgaaa
    50221 cgggtggaca cggttgtcat cagtacgcaa cattccgcat ccatgcggga taagcacgat
    50281 gaaatcgaag ctgccattcg caaatatgtg atcgacccgg tattaccgaa agagttggtc
    50341 gaccgagacc tgaaggtatt tgtcaacccc accggtgaat ttattatcgg cggtccgcag
    50401 ggagattcgg gcttgaccgg ccggaaaatc attgtggata cttacggcgg tatgggacgg
    50461 cacggcggcg gatcgttcag cggtaaagac tgtaccaagg tcgatcgttc agccagttat
    50521 gcgacccgtt gggtggctaa aaatatcgtc gcagccggct tggcggaacg ctgcgaaatt
    50581 cagatttcct atgccatcgg tgtggcgaaa ccgatcagca tcaatgtcga aaccttcgga
    50641 acggggaaga tatcggacga ggaaatcgtg cggctgatcg atcgaaattt tgatttacgg
    50701 ccgggcgcga ttatcaaacg gctgaatctg cgccgtccga tttatcggaa gacttcgaat
    50761 tacggccatt tcggtcgtga agatgcggat ttcacttggg agaaactgga tatggcggaa
    50821 gctttacgcc gcgatgccgg actctgaagc aagaagcgat ttttgcaaaa ttaaaaagca
    50881 atccccattt tcggtttgcc aaccgggaat ggggattttt atatctgcaa atcgcgaaga
    50941 atgt
//
LOCUS       DAOSMM010000008        50137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_739_length_50137_cov_15.523481, whole genome shotgun sequence.
ACCESSION   DAOSMM010000008 DAOSMM010000000
VERSION     DAOSMM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 50137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50137
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_739_length_50137_cov_15.523481"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1772)
                     /locus_tag="PK898_02465"
     CDS             complement(63..1772)
                     /locus_tag="PK898_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOU18929.1"
                     /translation="MDQRFKIIQPTNIIQAELPDGRLIEGPRGGTVEEFLQPIVSELE
                     FPLVAAIVNKELRELSYPLISNANIEPVTTAQSDGKNIYRRSLVYLLSATFERMMPDY
                     ELMVDHSIHSGGLFCRIKGPELQDPKGFYKKLQTEMQATIDANLPILRVKLTIEEAQA
                     LFTSKHQQEKADLLKYRKKDYLTAYKLGDFVDYHYGYMVPSTGYLKVFGIEPAGRPNE
                     FFLRYPSDGKSNVIQKLDESSKMMSEFRRYGDWLQRLGIDSIASLNQAIESDRIQEVI
                     LVSEALHDQKFVQIARDIVERKNEVKVVLIAGPSSSGKTTFSKRLSIELLALGISPIP
                     IEMDDFFVDRERSPRTPDGNYDFESIDNLNLDLMDRCTDRLIAGEEVQLPRFDFFDGK
                     SKPGKILKMKKDQLLIFEGIHGLNPKLLPGISPEKKYRIFVSPLTQLNVDRYNRVSTV
                     DTRLIRRVVRDYRDRGYSAQETIARWESVREGEEKYIIPHQRLADEYCNTSLVYELSA
                     LKPMAETALRQVQWGSWEYIEAKRLLSFLEWVLPLPDGLVPANSILAEFIGGSNLKNF
                     TVW"
     gene            1844..2476
                     /locus_tag="PK898_02470"
     CDS             1844..2476
                     /locus_tag="PK898_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOU18930.1"
                     /translation="MTKMTIFIILTVLLLIRIIIGLWAKKRIFSLTSIPRNEYGIVFG
                     AGLEKTGLPSNVLMERIQTAAMLYRAGKIRKVLLTGDNHSLYYNEPQAMKIAAMTLGI
                     SEKDILIDPNGHRSFDSCRNAICDFNIHQAVLITQPFHLPRVLWLAVSVGIDAIGMPV
                     DLKRHRIDDEIWWRFRELFASVRAIIDGFIYKICYSVNEKRMNDRKGEIK"
     gene            2473..3324
                     /locus_tag="PK898_02475"
     CDS             2473..3324
                     /locus_tag="PK898_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU18931.1"
                     /translation="MSKDIGFFTLNNNVRIPKIGFGTYKAAENGDESVIKEAIRQGYT
                     LFDTASFYENEAIVGSAVKKSGLPRDHFFLTSKLWKTQMGYQNTLQAFNDSCKQLKTD
                     YLDLYLIHWPRPDLVCDWKKIVVETWKALEELYDEKKVRAIGVSNFLPHHLDVILNSC
                     KIMPAVDQIEFHPGYTQTETVNFCRSLRIQVEAWSPIGRARLIDNPLLKEIAAKYNKS
                     VPQLCIRFSLQNNVLPLPKASSPERMAQNLDVFDFEISEEDMEKIMAMPVTGWSGEHP
                     DAEPVAI"
     gene            complement(3333..4610)
                     /gene="mtaB"
                     /locus_tag="PK898_02480"
     CDS             complement(3333..4610)
                     /gene="mtaB"
                     /locus_tag="PK898_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOU18932.1"
                     /translation="MKVFLDSVGCRLNQAELEQFAAKFKQYGYEIADSADEADLAIIN
                     TCSVTKEAASDSRGKIRNAARSGHTQVIVTGCWSETEPEAASKMPGVTQVIRNHEKEQ
                     IASIVTGIPEPFINPVSIKREILPGEHFRNRAFIKVQDGCDNYCTFCLTRLARGKSRS
                     VAAETILQDIRAAENGGGKEIVLTGVNLGAWGMDLNPPQGLRDLLNRLLAETSIPRIR
                     LSSLESWNLDEAFISLWENKRLCPHLHLPLQSGSEAVLKRMARRNSLPDFLRLIQCVR
                     QINPLFSVTTDIIVGFPGETDQDFQRTLDFVRTAAFSGGHVFTYSVRPGTAAAKLDGR
                     IPKDVKRERSKILREQLDEQRKSFGMKMIGTTTNVLWESAEPINKENCRLKGFSENYL
                     PVKCLGSQNLLNQISNVEVTDFDGISLAGKIIS"
     gene            complement(4607..5434)
                     /gene="proC"
                     /locus_tag="PK898_02485"
     CDS             complement(4607..5434)
                     /gene="proC"
                     /locus_tag="PK898_02485"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOU18933.1"
                     /translation="MNSEKMAIIGIGVMGKAILTGILKKRLISPQNISVYDTDADKMR
                     SAYEELGVNPSDSGTDAIRDADVILIAVKPQFLSEVFGELKNKIHPDALVISIVAGVP
                     IEQFQKGLDHERVVRVMPNTPAQIGEGVSGWFASREVAADQRNLAQEILACTGYAFEL
                     KSEGQLDQITAVSGSGPAYVFLFIEAMIDTAVHLGLSRPLATEIVIRTVKGSTDYLAM
                     RKEHPAVLRNEVTSPGGTTAEAVFELEKLGFRNAIAEAMWACYERTIELGKGSEGSH"
     gene            complement(5438..6337)
                     /locus_tag="PK898_02490"
     CDS             complement(5438..6337)
                     /locus_tag="PK898_02490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU18934.1"
                     /translation="MNNKFLPEVKLLESPDWKDYMLLDSGAGRKLERFGRYILIRPEA
                     EAMWSPLLPETEWNKASAEFKPSPEENGGHWIFYEKDIHPWNIGYKNLKFQLMLSNSK
                     QVGVFPEQACQWDWVSSLIRNSEKPVKVLNLFGYTGAITMAAAEAGAEITHLDASKKA
                     VDWAKENQKLSSLEQTPIRWIIDDGLKFVQREARRGSFYRGIILDPPKFGRGPKGEVW
                     EFYKNFPELIQACRNILDSEALFLLVTAYAVKASSVTVYNTVNEVMKGMGGSVSAGEV
                     CLKDKSGGRLLSMAVYSRWQKEK"
     gene            6480..7394
                     /locus_tag="PK898_02495"
     CDS             6480..7394
                     /locus_tag="PK898_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18935.1"
                     /translation="MTKIIEVDKLSKSYGPVKAVQDISFYVEKGDLFAFLGPNGAGKS
                     TTIDIITTVLMPDKGTVIVDGLVLGKEDDKIRSSIGVVYQDNLLDRLLTVEENLFFRA
                     GFYGFNKDRIRESVKNAILTTGIQDYAKQRYGKISGGQRRRADITRALINTPQILFLD
                     EPTTGLDPQTRKNLWEVIRKLQRDREMTIFLTTHYMEEAAEADYVVVIDEGKIAARGT
                     PFELKEKYTSDLLKLSFKDEIKGLSDLADWGITYTKAGDQFIIRLPHTTDALPILDRC
                     RENLNGFEVLNGTMDDAFIGITGKEIRE"
     gene            7391..8248
                     /locus_tag="PK898_02500"
     CDS             7391..8248
                     /locus_tag="PK898_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18936.1"
                     /translation="MIALASRNIKLFFRDKAMVFFSLLSVFIIIGLYVLFLGNVWTSE
                     FPNQEYAKFIMNSWIMAGLISITGFTTAVGAFGIMIQDRELNIEKDFLSAPVKRSSLA
                     AGYLLSAYGISVIMSLIAFILGEIFILAEGGELLGIVAIFKIIGIILINSFMSVSIIQ
                     VIIGFIHTGNAFSAFSTIIGTLIGFLTGIYLPIGQLPTGVQTLIRIFPVSHSASLARQ
                     IFMDVPLKIGFNGVPEAAVNGFKELMGVTFFIGSKEITPWMSLCYMLLIGALFFAIAV
                     MNLSKRKLE"
     gene            complement(8411..9703)
                     /locus_tag="PK898_02505"
     CDS             complement(8411..9703)
                     /locus_tag="PK898_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU18937.1"
                     /translation="MKTLFSNVDILTPKYSQGVIRDGYLGITNDIISYIGETKPIEEY
                     DVERNFYNCLIIPGLYNMHTHTPMAALRGLGTNLPLDRWLFEAMLPIEQKMTPADKSV
                     GVRLEMMEMLASGVVSFSDMYHYPNHYIEDVIQSGMKANLSFPIMAVMASDSDKNKEI
                     MRLSVEFFDEYNGSADGRIMTDFAIHAEYTNNVEIVREYSEICRGKNGRMHIHLSETK
                     KEHEECKKRYGKTPAAWFYDLGTFDNPTIAAHCVMVEPEDILLLKEKDVTVVHNPSSN
                     MKLGSGVMPIRKMKEKGLRITIGTDSSASNNNQNMIEEMALTALIHCGFSQDPTVLSP
                     NEVLTMATLNGAAAQGRSNCGELEVGKKADLAVIDLDKPHLMPIHDYPSLIVYSAQAA
                     DVIMTMVDGRTLYEKGEFLSIDKDRVKFDLKKSLERLF"
     gene            10185..12914
                     /locus_tag="PK898_02510"
     CDS             10185..12914
                     /locus_tag="PK898_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU18938.1"
                     /translation="MEKKTLIVNGIRQVVIHNPDAMLADVLRNQLHVLGVKKGCGQGH
                     CGACSIILNGKLVLSCIHKMSRIPDESTILTVEGIGTPGHLHPIQTAMKFHSAAQCGF
                     CTPGFVISSYALLQENPNPTRQQIRDWFTEHHNVCRCNGYKPYIDSVIDAAAVLRGEK
                     SIEEITYKEPKDNKVWGTRRPRPSLDAKVTGTWDFGADVAAKMPSNTLHCALVQSQVP
                     HANIISVDTSEAEKAEGVFKVVTAKDVKGRNQITGLITFPTNKGDGWDRPILCDKKVF
                     QIGDAIAIVCADTQEQADAAAKLVKVELEILPAYMNAPAAMAEDAIEIHPGTPNIYFE
                     QKNVKGNDTKPIFEKAAYTVEGNYYLQRQPHLTVEPDNGFAYIDEDGNLTIHSKSIGI
                     HLHAAMIAEGMGVPLDKLRIVENNAGGTFGYKFSPTMEALVGVACLATQRPVVLIYNY
                     YQHMIYTGKRSPMFLKCRMAADENGKLLAMEGDWSVDHGPYSEFGDLLTLRVSQFMGA
                     GYNIPNIRSIGRTVCTNHIWGSAFRAYGSPQAFLASESCIDELAEKMGIDPFELRYRN
                     IYREGSTTPTGQKPDSLCFEEMFDTMRPKYEAAKAKAKAESTPEYKKGVGISLGVYGC
                     GLDGADSSEAIAELSPDGKVIIYSSWEEHGQGNDAGALGTAHEALRKAGFKPEQIKLV
                     FNDTKITPNSGPAGGSRSQTMTGNAIRIACENLLDQMRKADGSYRTYDEMKADGKETK
                     VTGKFVMSEATSCDENGQGRPFMVYQYGLFMAEVTVEIATGKTKVDKFTCFGDCGVIN
                     NRAIVEGQMLGGCAQGIGLALSEDIEDYNKHTTMRGAGIPYCNDIPDEWDIEMFENPR
                     EYGPFGAGGVGELPLTASHVAVINAINNACGVRIRHLPAYPEKVLEGLKALNK"
     gene            13033..15330
                     /locus_tag="PK898_02515"
     CDS             13033..15330
                     /locus_tag="PK898_02515"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOU18939.1"
                     /translation="MDQQFMRTMESICIQNEPPACVTACPLHVDSRTMLKLVASGKFE
                     DAYSLYQKNVPFPSILSKICNSPCLSKCRLSEIGEGIQIRPIEAFLNQYADPSFLPKV
                     LPEKRQKAGIFGSELDSLSAAYDLRRKGYQVTILDADAVLAKNLRAFNEEVLPSVEIE
                     NAIHLLEKIGVKIQTGFEMPRFEELSRNDSGFFRIGDSEFQCIYLPPLSGELSIDSIT
                     RMTVTEGIFSGSASAGHWVDQMSDGRRAAISMDRYLQKVSLTASRENEGAYECRLFTS
                     LKGIQPSESFISTDIKRTGQADIIKEAARCIQCQCLECAKGCEFIRHYQDSPRTYVRQ
                     IYNNISICTGLRQKNKMINSCSMCGQCEAICPNHIPFQKVVREARETMVATKKMPPSA
                     FDFALRDMAFSNSEKFRLAKVPVDSKACKYAFFPGCQLPASNPSAIETIFSWLRDEMN
                     EPVGILLRCCGAIADWAGEKEKFNQARSELLADWESLGKPELIVSCPTCLKMFREKYP
                     EINVRSIWPLMAESDSLFNGKSRLNSIKMAIHDPCASRDQPDMQEAVRSLAMKLGIEL
                     EELPYNRELATCCSYGGNVWNSNPSLSKDTVEARISQNQSDYLTYCIMCRDFFLRSGK
                     PAYHILDILFDPKLIIEHRLPIRPDYSMRHENRTRVKRHLLKQYWREEMPEEAPYEDI
                     QLILNDPIRKLMEDRMILEEDIRQVLYHAKESGRAMINQETGHYLAHFKPSCVTYWVE
                     YLPKEDGFEIFNVYSHRMFVEEGNI"
     gene            15330..15542
                     /locus_tag="PK898_02520"
     CDS             15330..15542
                     /locus_tag="PK898_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18940.1"
                     /translation="MAQYVNEEDFIHWRCAKCNSELELRKVVVSYLDGSFPVELPVCK
                     VCGQVFIPESLATQKMLEIEQQMEDK"
     gene            15563..16180
                     /locus_tag="PK898_02525"
     CDS             15563..16180
                     /locus_tag="PK898_02525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU18941.1"
                     /translation="MNPAPGGIALTEKLLNLSLLQKGSMILDVGCGDGDACRFLSSEG
                     YRAIGLDIVRHSSGKMNSERIQGQINAIPILEETVDAVLMECVLSILPTDDGSLTEII
                     RVLKPGGILLFSDLYMRYPDEESEKNRIDSESDYPKIRLADELLNIFGESGLSVRYWE
                     DQSAVLQSLRLKFLWEGSASENKFPYKMKNIGYICLIAERVSASG"
     gene            16199..16651
                     /locus_tag="PK898_02530"
     CDS             16199..16651
                     /locus_tag="PK898_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOU18942.1"
                     /translation="MDDSDEIIEMGKQGFTCSQMLMLKALELQGKDNPDLVRSMTGFA
                     GGLGWTGDICGVLTGATAVLSLYAGKGQADDEDDPRLMFMIEDLVHWFHDKYGKIYGG
                     IRCTDILGPDAGKIDMQRCSEIIQDTFQYIKELLVNNGFDLSGESLDG"
     gene            16644..18011
                     /locus_tag="PK898_02535"
     CDS             16644..18011
                     /locus_tag="PK898_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU18943.1"
                     /translation="MDDYRELSQTLSVCPICLEKIPASYTVHPDGHVYLDKDCPSHGH
                     FSTIVWRDNPDFESWTMRSTPYPTEHPSTLIIRGCPYDCGLCPDHQQRPCCVLLEVTS
                     RCNLGCPVCYASSDEYSDLDPSLETIHGWFQMLLAAGGPFNIQLSGGEPTVRDDLPEI
                     IRMGKEMGFGFFQLNTNGIRIAQEKGYLESLSEAGLSVVYLQFDGTEDTIYRKIRGQD
                     LYQTKLKVMERCKEVGIGLVLVPTVIPGVNDENLGEIIRLAVSNLPIVRCVHFQPISY
                     MGRYEKTPENADRITLPELMRKIEEQTNGLIHIENFKPCSGQHPKCGFQANFLHTEDG
                     SLIPLSKFNPDEISSGCCSLSGDRQNSSQKAQNFVAKTWTLRNPEEGKQSDYKIVSDW
                     DSFLENRQKNHFTISAMAFQDAWNLDLERLKRCYIMIVSPDSRLIPFCAYNLSNRDGE
                     TLYRK"
     gene            18008..19306
                     /locus_tag="PK898_02540"
     CDS             18008..19306
                     /locus_tag="PK898_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU18944.1"
                     /translation="MTEPIHVPKMITPVQLEIERKLGIPDHSLTLEKLKKWQLDRLND
                     TLAFCMNHSPFYKEKLADFSNSIPLRSLEELQQLPFTTSDDISEKGHRMVCTSQSQIE
                     RIVTLNTSGTTGTAKRIYFTKDDQQLTVDFFAYGMSTLISSHSKVLVLLPCHPAGSVG
                     DLLSRAIRKIGAQPYSIGPFNNPDPINDLIASEKLDALVGAPKQVYTLVQSWKSSNRG
                     SCPIQALLLSTDYASPILKKSIESAWNCDVLEHYGMTEAGLGVGVDCYLKRGYHLREA
                     DLYFELIDKDGNPVAEGEPGEVVFSTLTRHGMPLIRYRTKDISQIIPGECPCGTVLST
                     YKWISKRMQNEDSLCTIAELDNILFQIPEINGFDAAAKKESLNLKIYSDKQNKEESIN
                     KISESILNTFPGVNKIEIELLAGFPSSAFSLQKKKIEYIS"
     gene            19520..20059
                     /locus_tag="PK898_02545"
     CDS             19520..20059
                     /locus_tag="PK898_02545"
                     /inference="COORDINATES: protein motif:HMM:NF040142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18945.1"
                     /translation="MRQIFLTQTETILLDIVIWVIIHLSIGYWTSRIPVNVFNPESWL
                     FRTRKWEKDGMFYEKYFRVKTWKDHLPSGAALYEDAYEVKHIGDYSTENLRTWILESC
                     RSEFCHYLMIIPGFFFFLWNTVNVGWIMVAYAFANNIIPIIMQRYNRPRVKKLLRYME
                     RSKKYSRQQTVSSGEALAI"
     gene            complement(20068..20787)
                     /gene="deoD"
                     /locus_tag="PK898_02550"
     CDS             complement(20068..20787)
                     /gene="deoD"
                     /locus_tag="PK898_02550"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU18946.1"
                     /translation="MKKANLTPHIECSPEDFAKTVLLPGDPLRAKMIAETYLTDAELV
                     NHIRGANGYTGYYHGKRVSVMGSGMGMPSIGLYSYELYHFFNVSSIIRVGSAGAIAPD
                     LKLYDIILAQGACTDSNYFKSYHIPGKFAPIADFRLLRQAAEECEKNNISFQVGNILS
                     SDVFYREGGIAETLQWRKMGVLGVEMESAALYMNAARAGKKALTICTVSDLLESGEAA
                     SAEERQNSFNDMVKIALAIAE"
     gene            21087..22292
                     /locus_tag="PK898_02555"
     CDS             21087..22292
                     /locus_tag="PK898_02555"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOU18947.1"
                     /translation="MNILIFNCGSSSQAFKVYQVSEKDQPKIIASGKAKNVATQTKAD
                     SFIEYRINDKQRKEIISLPSHRIAADVILTLLERENVTIDGIGHRFVHGGPRFNQSVK
                     IDENVFYELKNSFRFAPIHNPNSFSVIEVCKARLPEIPQYAVFDTAFHAKLPKESQVY
                     ALPKKLAGEYGFRKYGFHGLSYQNILSKMPDLLGKPIEQLNLVMCHLGTGGSSVTAIK
                     NGLPYDTSMGYSPLQGLIMSTRCGDLDPEIVLDLIRSGMSADEVDSVLNNQSGLIGLS
                     GYSSNLIEIIDESERGNEDCQLAYDAYSHRLKSYIGAFVWLLDGADAIVFTDDLGIKS
                     WKLREKVFKDSQNMGIRLDDGANQNASFEKPSLVSRNDSNIPIWVVPNDEEIVILNEI
                     LELIGNGTL"
     gene            22598..23299
                     /gene="pyrF"
                     /locus_tag="PK898_02560"
     CDS             22598..23299
                     /gene="pyrF"
                     /locus_tag="PK898_02560"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOU18948.1"
                     /translation="MKDVIIACDFKDAKTTLSFLDLFKTKKPFVKIGMELFYFEGPAI
                     VREIKARGHKIFLDLKLHDIPNTVMSAMQSLSKLDPDIIDVHAAGTTEMMKAAMEGAF
                     RSDGSKPLVIAVTQLTSTSEERMNRELLIQATMEETVLHYAQNAKEAGLAGIVCSPLE
                     AAIVKKGLGEDFITVTPGIRLPDSIKDDQARITTPVQAREIGTDYIVVGRPIIRAENP
                     VAAYDRFCHDFLGKA"
     gene            23341..24003
                     /gene="pyrE"
                     /locus_tag="PK898_02565"
     CDS             23341..24003
                     /gene="pyrE"
                     /locus_tag="PK898_02565"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOU18949.1"
                     /translation="MKREIAQHLLDIKAVFLRPNEPFTWVSGIKSPIYCDNRLTLSYP
                     EIRKDIENGLVSIIREHYPDCEAIFGTSTAGIPHAAIVADIMDLPMGYVRGNTKDHGR
                     NNQIEGRFEKGWKVVVIEDLISTAGSAVEVVQILRENGCNVLGIASIFTYGMQAGLDN
                     LRKAEVQNYSLTDFDTLIEVELENGSLSRADVNKLLAFRANPSDSSWQFCTSAVENPQ
                     IY"
     gene            24316..24765
                     /locus_tag="PK898_02570"
     CDS             24316..24765
                     /locus_tag="PK898_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU18950.1"
                     /translation="MINQIHSLLSAFTTWTGLSMNYSMHGFVRFIRENGLSLSQISTL
                     FQLYHCGTSAVSQISSNLDISNAAVSQLLERLVQQGLITRSENPLDRREKQLVLTNKG
                     YELLRDSAKACSAWQEKLISTLTPEEQNKVSEALEILIDKTKQLPNA"
     gene            24845..27091
                     /locus_tag="PK898_02575"
     CDS             24845..27091
                     /locus_tag="PK898_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18951.1"
                     /translation="MQRLLKYLKPFIFLLILAIALLFLQANADLALPDYLSKIVNVGI
                     QAGGIENAIPQAIRQTEMERLMLFFSAEEKERVLNDYTLVDQSSPDYNRYLSQYPALK
                     DGAIYVLNPIDQAETDWLNPVMAKALLVVYGIEQGMADPTKVTALGQMNFNGFDISKL
                     PPGTDLFKYMGQMPPAALSQISESINKRLEAMGSSSLIQAAAAPIQAEYAALGMDVGA
                     VSHKYILNTGKLMLLLTLLSVLCNIGVGFLSSRIASGFSRNLRKAVFSKVESFSSGEF
                     EKFSTASLITRTTNDITQIQMVVIMMVRMVFYAPIIGVGGVMRAINKDSSMWWILALA
                     VGILILLIIIIFNIAVPKFKIIQSLIDRLNLITRENLSGMMVIRAFNRQDFELKRFDK
                     ANRDLTSTSLFVNRVMAVMMPIMMLVLNGISLLIIWVGGKQVAASNMQVGDILAFMQY
                     GMQIIFAFLMLSVMFIILPRAQVSANRIADVLETEPSVVDPENPQSFPPDGKGLLEFR
                     NVSFRYPDAEEYILHDISFVAQPGKVTGIIGPTGSGKSTLVNLIPRFYDVTEGAILID
                     GVDIRNVTQHELRKRIGFVPQKSSLFSGTIESNLRFGNENAPEELLYKAAEIAQATEF
                     IEAKPEKMDAEISQGGTNVSGGQKQRLSIARALVKQAPINIFDDSFSALDFKTDAALR
                     QALKQQAVPGTLLLVTQRVASIMNADQIIVLDEGRIVGKGKHQDLMKTCEIYKDIALS
                     QLSMEELA"
     gene            27088..28941
                     /locus_tag="PK898_02580"
     CDS             27088..28941
                     /locus_tag="PK898_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18952.1"
                     /translation="MNTETTNRQSMPRHGGGRRGRMTGEKARDFRGTMIKLIKYLSVY
                     RNSILIVVLFAAISTVFTIIGPKILGQAITKLFEGSIAQLTGTGSIDFDAIGKILLTV
                     AGLYLTSAAFSYGQGWIMAGVSAKITYRFRKDIAEKINRMPLGYFESTNHGEILSRVT
                     NDVDTVSHTLNQSLSQIITSSITVIGVLIMMLSISWLMTLVALAIIPLSLLVVSLIVK
                     QSQKYFKQQQDYLGHVNGHVEEMYGGHIVIKAFNGEEKSIQRFNQLNDKLYEAAWKSQ
                     FFSGMMMPIINFIGNIGYVAVCILGGYLAVQKTITVGDIQAFIQYVRNFTQPITQLAN
                     ISNVLQQTAAAAERVFEFLNEAEESPETENPVDPETVRDRVEFKNVRFGYNPQKIIIK
                     NFSANVDSGQKIAIVGPTGAGKTTIVKLLMRFYDVTDGAILIDEHNVKDFYRKDLRQM
                     FGMVLQDTWMFNGTIIDNIRYGRPDVSDEEVIAAAKAAHIDHFIRTLPEGYNFVLNEE
                     TTNISQGQMQLLTIARAILANPKMLILDEATSSVDTRTELLIQKAMDHLMANRTSFII
                     AHRLSTIRDADLILVMRDGDIVEQGKHKELLARGGFYAELYNSQFEMGQTI"
     gene            29025..29666
                     /locus_tag="PK898_02585"
     CDS             29025..29666
                     /locus_tag="PK898_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOU18953.1"
                     /translation="MEEKLFSVTISHQIGSGGAYIGEKLSERLGIPFLDRAILKEVSN
                     QLNLAESELEHREERLSSFWENFNRLVAYTNPTTSLTSQTYFPTDKDLFQVECDTIKR
                     IAEKSSAIFLGRCGYYVLRDHPRKVSIMLIADQSSRVKRLQELYKISEKEALEMIRTN
                     DRERSEYIRTFTKQDWLDVRLYDICINTTSVGLDCAADLAENCIRAKLRLTEK"
     gene            complement(29713..30480)
                     /locus_tag="PK898_02590"
     CDS             complement(29713..30480)
                     /locus_tag="PK898_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU18954.1"
                     /translation="MQIVYLIMIGLITGCVVGLTGASGVVVVVPLLTVLMGFPVHMAI
                     GTSLFIDTITPLFVAWSYYKSGNINLKAGLWLALGSVTGAQIGSSFANEAISGQGMSI
                     GFIIIIFLMAIGMWVKSTRPERPAVENSQIKPFTVKNAAISFGIGLALGIFSGMFGAG
                     GGIAFLIVMITVLKYSTHVAIGTSSLIMSLTAASGTIGYAIHGNVNFGSGLIIAIGSV
                     VGGLLTARLANKVNEQTLEKLIGCVFAGLGVIMLTIE"
     gene            complement(30521..31369)
                     /locus_tag="PK898_02595"
     CDS             complement(30521..31369)
                     /locus_tag="PK898_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU18955.1"
                     /translation="MKQKVAIVTDGACSLTLEQANEYGIHMAPIYVNIDDKTYIAGVN
                     LGGPEFYQLQRASKKLPTTSQTTAEDFLKIFTKLADEAEEIVTITISSKMSATIQSAE
                     MAKKQFTKVPVHIIDSESVSLGQGMMAIAAAQAAKEGQDAESVIHLVEEIKKRMNVIF
                     TVRTLEYLYKGGRIGGATAFLGSALAIKPILYIKDGVIEPLEKQYTRKKSIMRLVEIM
                     KERIGDKPVHVAILHGDAEEEAYVLENQIREKFNYVQLIKAEIGPVIGVHAGPGTLGL
                     VFYSME"
     gene            complement(31390..32073)
                     /locus_tag="PK898_02600"
     CDS             complement(31390..32073)
                     /locus_tag="PK898_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU18956.1"
                     /translation="MMKIRYLGHSCVEIIGRHHILIDPDFTFPPQPGVEYICLTHAHK
                     DHIGKVAEVQSGTILASADICEIVERRGIPSSRIRPVLPGQEIDSVTILPGYSLTNQP
                     AYVFMNLLTRRRLPEPGGTPLSFLVRDEISVLHVGDANEAPLNVHPDIFCLPWRTSPV
                     GDKKYKENLIKLANSFQPNYLLPIHYDLPGSEADPAEICKYTTSMKVLLGKFWFLFHN
                     REEFTTEDF"
     gene            complement(32190..32867)
                     /locus_tag="PK898_02605"
     CDS             complement(32190..32867)
                     /locus_tag="PK898_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18957.1"
                     /translation="MKLSGKITWVGIYAIAMAFLETAVVVYLRRIYGITDLMLSVPPF
                     DPEIAAVELGRELATLVMLLAIGWVAGERIQSKLSFALIAFGIWDIFYYIWLALVISW
                     PRSIFDTDLLFLIPLPWWGPVIAPVLISLLMIIGGILIVRREDQGIIVRLRISDGIFL
                     GLGILIMLYVFMQDAISILPATSEELSSLKPSSFNWFVFMIGYILSAITVWRIVKKKP
                     SHLEKNI"
     gene            complement(32881..33300)
                     /locus_tag="PK898_02610"
     CDS             complement(32881..33300)
                     /locus_tag="PK898_02610"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOU18958.1"
                     /translation="MKSLPKTGSQYVFGSCGLKADDVVWQTLPNGRIEIRYTFQLFQE
                     GPKGYVHGGASAALMDEALGIAVWESGFHGATVHLELNYRKPIPLGEEVRVECRVTSE
                     GTHSRNAEGIIYRSDGSIAVEGKGKFALAPQLFGISS"
     gene            complement(33301..34017)
                     /locus_tag="PK898_02615"
     CDS             complement(33301..34017)
                     /locus_tag="PK898_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU18959.1"
                     /translation="MPNQGIDVQWFISVILLIIGSYLLGSIPSAYLAGKWIRGIDLRE
                     YGSGTVSGSMVWEHVAKWAVVPVGLFDIFKGFLPTWMTLRFGLGESAAVMVGLAAVIG
                     HNWPIYLNFHGGRGLSPFLGNLLALFPLGCFIMLLSLGIGCLLTFAPLPLITILLMPV
                     FANIFNGPNAILGLSLGMLLITVVKRLEANRRPLPKDPEERKKVIFLRIFYDRDIPDQ
                     KEWIRREPTNQKSVPQHKEP"
     gene            complement(34061..34927)
                     /locus_tag="PK898_02620"
     CDS             complement(34061..34927)
                     /locus_tag="PK898_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU18960.1"
                     /translation="MMRKSVVVTDSAAQVPKEAIDKYGIEVIPLLISFDNKNYLDGID
                     ISAHELYQKMRNDKIVPKTSAPTIMQFYQSFEKHIKEGVSEILFVSLSSKLSSTYNAA
                     LDAAKMIREKYHDCEIVIYDSLMATIAQGFLAIEAAQRFEEGMSIKNVVTFLDGARKR
                     TGIAAALDTLEYLARGGRIGKAQYVLGTAIQILPILGIDQEGIVAPVANLRGKRKVIP
                     TMVSLIKKQIGAYHKARFAVMEADAHERAEALQKAAMEAFPKNEFPIVEFTPVMGAHT
                     GPGLIGLAYYWE"
     gene            complement(34927..36219)
                     /locus_tag="PK898_02625"
     CDS             complement(34927..36219)
                     /locus_tag="PK898_02625"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU18961.1"
                     /translation="MKKKSSIGISLFIILCLFLSGCENLNGQSTSLKASGTISAVQVD
                     VAPQIGGTVVSVSTEEGAQVKKGDELFRIDDSLLKAQRDQADASVTLAESALATAKTQ
                     YDLAVDSARQQERQTRINSWNTEQSSQFKLPVWYFDKTEKLASAKVEMDAAKADLDEE
                     KASLEKILNDKASAGFLNAEKRLANAEASFLIADQVLTQATNAKDKEELQDFAQDQYD
                     TAKTELDSAQSNYDRLLTTQAAKDVLEGRARVRVTQERYDRALDYYNSLLSGDQSLQV
                     KVAESNVSQAEAGLKQAQAALSVIDVQLEKTIVKAPVDGIILARNIEVGETIAPNAVV
                     LTVGQLENVNLTVYIPETEYGKVKLNEKVSIQVDSFPDETFTGAVVYISDQAEFTPRN
                     VQTMEGRRTTVYAVKIDVPNPDLKLKPGMPADVTFEEK"
     gene            complement(36266..37402)
                     /locus_tag="PK898_02630"
     CDS             complement(36266..37402)
                     /locus_tag="PK898_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18962.1"
                     /translation="MRQSFHRIMTIVRKEWIHIIRDPRTLSLVIIMPVMMLFLLGYAV
                     ANDVKDIPLAVADLSHTDASRTLIDKLTGSEFFLYTHAAQSEDEILKMLDEGTVKAGL
                     FIPENYGREIYTGGSTTIQFYIDGTNPTIAQTAQLAAETISQSLSQEILIQRLERSGT
                     GMQISLPVDARLRYLYNPDMKRMNFMIPGLVAIILQIQALLLTAFAIVREREQGTLEQ
                     LIVTPIKSWELMLGKILPFVVVAFINVVMTVAVGAVWFGVKVVGSYALLAGLSLIFLL
                     GSLGLGVFISNISKTQMQAMYMASFIMIPAFILAGLLFPRENMPGLAYYAGYLLPVTY
                     FLEIVRGIILKGADFVILWKWIWPMAVFSIVVFFASVLMFHKRL"
     gene            complement(37413..38366)
                     /locus_tag="PK898_02635"
     CDS             complement(37413..38366)
                     /locus_tag="PK898_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18963.1"
                     /translation="MSGEKSVQVSELTRTFGNFVAVDHVSFDIPKGEIFGLLGPNGAG
                     KTTTIRMLCGILKPTSGEARILGYDVAKKPEEVKKRIGYMSQKFSLYNDLTVAENLKF
                     YADLYGIPQETMKTRKVELIEMAGLRGHEKQLTRDLSGAWRQRLALACAIVHDPPMLF
                     LDEPTAGVDPVSRHDFWELIYTLAGRGVSVLATTHYMDEAEFCNVIGMMYRSRLIALN
                     DPDSLKEAMNGVLFEIDCDEPAHAEQLLKKRDNIQDVAAHGVLLHVLVKDESDKMMLE
                     NTLQENGIKIARIERILPSLEDVFVSLVNQENRMHVRAGIE"
     gene            complement(38363..39310)
                     /locus_tag="PK898_02640"
     CDS             complement(38363..39310)
                     /locus_tag="PK898_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU18964.1"
                     /translation="MTTTIEAYHLIKRYPNQGKMDPAALDGVDLKIESGQLYGLVGPD
                     GAGKTTLIRILSTVILPTSGTAKVAGYDILKQAQKVRPHLGYMPQAFSLYPDLSVIEN
                     LRFFADINGVPKSKQKERIDDLLDFARLSDFTSRRSENLSGGMRKKLALACALIHEPE
                     ILLLDEPTTGVDPVSRRELWQLLAKVIERGVTVLVSTPYMDEAERCDVVSIIFQGKML
                     VSGSPLDLEKQLPFQMIEVKAKPRGVLRKVVDNSKEVLSWRAVGDRLRLSVNDPVGAQ
                     SHLRSELIEAGADVSILREAKIGMEDVFIHLMEQKEKQA"
     gene            complement(39327..40052)
                     /locus_tag="PK898_02645"
     CDS             complement(39327..40052)
                     /locus_tag="PK898_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18965.1"
                     /translation="MELTRRQEKFIHLFVDLYREMHKPIHYSLLAKKIGVSSFTAYDM
                     LRLLEKKGYVTSKYQINSNKPSVGRSEIVFTPTSLAYSRFPEEIPDTKTENPNPDETE
                     IAEKVQPNQIIQDHALSEIVLNQISIDTPDNLRFCLNVLTDLITGLKSCSSCRFLLKW
                     ISQFVGKKGIITTRSGLLFFGGFIFGMVTREKIIDLDNDRSIVPYMERYRSLVMEMDP
                     QLCKQLGNDISEMFTFIIESELI"
     gene            40450..>41272
                     /locus_tag="PK898_02650"
     CDS             40450..>41272
                     /locus_tag="PK898_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18966.1"
                     /translation="MKKMRWFIVILIALFTAGSVSAQDLQATNQALELEINILKLQAT
                     KQALEAEVGQQNEPQPSEPIEDASGGMMNWGSQNSPYVPTPTPFNNVSVPISQQNFPA
                     LVRPPIPQGPPPTWDKPRTNRITRIYVGNSGQFKTMADALKNIPDKAGEVIIYLTSDT
                     EEPDDGFGIPIDKEITVVRIASDTGAKRTAWPADRSFWFFCNGVPLIIEPEVEFAGKS
                     MIMGGALTYARHNVQTPKSVIIINGSAWWVYAGGQSDREGHSSTVDNALVIINGNV"
     assembly_gap    41273..41372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <41373..41660
                     /locus_tag="PK898_02655"
     CDS             <41373..41660
                     /locus_tag="PK898_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18967.1"
                     /translation="EGHSSTVDNALVIINGNVDRVHAGGRAIYGETIVNRATVVVNGK
                     ANEVYCSGYTENPSAKTTVGNANMLIYGWYGIYGLARGQGQAYLLNPAGCY"
     gene            complement(41758..41831)
                     /locus_tag="PK898_02660"
     tRNA            complement(41758..41831)
                     /locus_tag="PK898_02660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41795..41797),aa:Val,seq:tac)
     gene            complement(41894..42235)
                     /locus_tag="PK898_02665"
     CDS             complement(41894..42235)
                     /locus_tag="PK898_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HOU18968.1"
                     /translation="MERKIETLNEYQAQCMRTTIGGMDEKMTHLAWSLALAGEAGELA
                     NRTKKVFIHGHPYDQDRVIEELGDILWYVAVYAHDIGVDLEEVAERNIEKVAKRYPNG
                     FSAEASINRKE"
     gene            42359..44941
                     /gene="clpB"
                     /locus_tag="PK898_02670"
     CDS             42359..44941
                     /gene="clpB"
                     /locus_tag="PK898_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOU18969.1"
                     /translation="MNFDKYTQKAQEAVAQSQQIAIEFSHQVIEPAHLLLALLRQQDG
                     TVPAIVTRVSGSVAGLLNDVEKSLANQPKVYGSSGNLSMSRTTADVFSNAEKIAKGMQ
                     DEYVSTEHLFLALCDSVEGRRLEQFGLTKDSILKALMSIRGSQRVTSQNPEDTYQSLE
                     KYGRDLTAIARQGKLDPVIGRDDEIRRTIQILSRRTKNNPALIGEPGVGKTAVVEGLA
                     QRIVKGDVPEGLKNKRLVQLDMSALVAGAKYRGEFEERLKAVLKEITDSEGQIILFID
                     ELHTIVGAGAAEGSMDAGNMIKPMLARGELHLIGATTLNEYRKYIEKDAALERRFQTV
                     FVDEPSVEDTISILRGLKQRYEVHHGVRITDAAVIAAATLSNRYITDRQLPDKAIDLI
                     DEAAARLRTEIDSKPQILDSVDREIMQLEIEKQALQKEHDQASKDRLAKLEEELANLK
                     EKSNQLTARWHTEKEAIQNLKTVKEKLESLHIQIEQAERANNLELAARLRYGELPALE
                     KQLAAYEEKLKEIQKEGALLKEEVDEEEIAQVVANWTGIPVSRLMESEKQKLVHMEER
                     LHQRVVGQDEAVKAVSNAVRRSRAGLQDPNRPIGSFIFLGPTGVGKTELAKALAEFLF
                     DDERAMVRIDMSEYQEKHTVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVILFDEIEK
                     AHSEVFNVLLQVLDDGRLTDGQGRTVDFKNTVIIMTSNAGSELWMNGGKVVSRDAVNR
                     VLQNTFKPEFLNRLDEIVVFHSLTKEDLKQIVDIQLKHVRDLLIQRGLMLDITPKAIE
                     FLVETGTDIEFGARPLKRAIQHELQDPLAMEILNGKYVQGDTIQVDLAPEGDHLVFSK
                     KQTS"
     gene            45097..45726
                     /gene="upp"
                     /locus_tag="PK898_02675"
     CDS             45097..45726
                     /gene="upp"
                     /locus_tag="PK898_02675"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOU18970.1"
                     /translation="MSNVTITNHPLIEHKLSILRDVHTNPTVFRHLVNELGALLCYEA
                     TRDLPLCPVEVQTPLSMTTAYEMKESIALIPILRAGLGMVEGIWDLIPNAHVFHIGFY
                     RNEKTLQPVEYYNKLPKDHPFNISFILDPMLATGGSAIATTNIVKKWGAQTIKFVGII
                     GAPEGIKALQEAHPDVDIHLAAIDDHLNDKGYIVPGLGDAGDRQFGTVF"
     gene            complement(45733..46284)
                     /locus_tag="PK898_02680"
     CDS             complement(45733..46284)
                     /locus_tag="PK898_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU18971.1"
                     /translation="MYSDKLALFPKTILTQRLQLRPITRADENDIFEYASNPEVARYM
                     SWSAHRTLADTEEFLKYIDQVESEKLQIDRGIVWRENNKMVGTIAFVSIDLALRVVEL
                     GYCLSNQYWGRGIIVEAANAMIDAAVRTLDIHRIEAECEIDNFKSERVMQKLGMEQEG
                     VMRKRLPVHGVYCDAKLYAKVVG"
     gene            46560..47156
                     /locus_tag="PK898_02685"
     CDS             46560..47156
                     /locus_tag="PK898_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU18972.1"
                     /translation="MTNPVITIGPRATLETAKDLIDKKSIHSLPVVDRQGRLMGLITK
                     DRLEDFIQKAKQADEPMPLVEDVMVQQVLTVTENTPIEEAARIISDYDVKSLPVVRGN
                     FVVGIITASSLIRIFLELTGARDQGVRLTIMVEDVPGKILAVIQKIVELNGNISTFST
                     YYSDEKNYRIMTLKISGVEKYKLKQEIAPIVYKIIDIR"
     gene            47367..>50137
                     /locus_tag="PK898_02690"
     CDS             47367..>50137
                     /locus_tag="PK898_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOU18973.1"
                     /translation="MIALDIETTGLDPNLDAVTEIAALRFKGARVEDTFQTLVNPNRP
                     IPSNITQLTHITNEMVRNAPQIGEVIGKVAQFVGNDIIVGHNISFDLNFLRLKGILQK
                     NLSYDTYDLASILLPDAPRYNLGVLAQRMGIIVRDEHRALADCYTTYQVFNRLIERAE
                     SLPAELLADIVNLSEGIHWNEAELFRQIIQKRIQNGEKIKPIYKITFPTFLNEGGNNV
                     DPLKPSENPIPLEEDEMAAILEYGGAFSKYFPNYEQRSQQIEMLRTVTTAFSKGKHMF
                     IEAGTGTGKSFAYLIPAFHWAKQNGERVIVSTNTINLQEQLIRKDIPELNNVLQEDFR
                     AAVLKGRSNYLCPRRVQMLHSHGVENADELRVLSKVLVWLYEGGKGDRGEINLNGPID
                     REIWERLNADFEGCRPNQCPHYRDGQCPFYAAKAKALKSHVIIVNHALLLTDMAYGND
                     VLPEYKYLIIDEGHHLESAATGALSVRITQTDLSHQLQELGGYGRGLLGRMDNKLAEK
                     LKPDEYQLFVNATTFISENGALAEEALKSLFETFQFFMKEAREGNPVTVYGQQLRITS
                     AIRTLAGWADIEIIWDKTEKYIRELIDSAEKIDRSLMNLDDIDEELRELIDSFHGGLK
                     KLSESKKMVNNLIVKPDPETIYWIEISPVNNRLSLNSAPLHVGNLIEKMLWHEKESII
                     VTSATLTADNSFDYIRDRLSADEAEELVVGSPFDYENSVLLFIPNDIPDPNQGSAQHM
                     VDTSLINLCKAVGGRTLALFTSYAQLKRTSTDIAPEMQKAGITIYEQGEGASASSLLE
                     TFRETDQAILLGTRSFWEGVDIQGEKLSVVVIVKLPFDVPSDPIFSARSEGFENPFSQ
                     YSLPEAILKFRQGFGRLIRSQSDRGVVVILDNRVLTKRYGREFLNSIPKCTERIAGIR
                     ELPREAQRWL"
BASE COUNT        14516 a        10988 c        10611 g        13922 t
ORIGIN      
        1 aaatccgaca aaagggaaca aaaagaatct taataatgct ttttgttccc tttttgtttt
       61 gattaccaaa cggtaaaatt cttcaaattg gatccgccga tgaattccgc caaaatagaa
      121 ttcgccggga ctaacccatc gggtaaaggc aaaacccatt caaggaatga gagcaaacgt
      181 tttgcctcga tatattccca ggatccccac tgcacctgtc ttagagcggt ttcggccatc
      241 ggtttcagag cagacaattc atagaccaat gaggtattgc aatattcatc cgccaaacgt
      301 tggtgcggga taatgtattt ttcttcgcct tcacggacgg attcccaccg tgcgatcgtt
      361 tcttgagcgc tgtagccgcg atcccgataa tctctcacca cccggcggat gaggcgggta
      421 tctaccgtcg agacccggtt atatctatcc acatttaatt gtgtcagggg cgatacaaaa
      481 atccgatatt ttttttcggg ggatattccc ggtaacagtt tcggattgag tccgtgaatt
      541 ccttcaaaga tcaataattg atctttcttc atttttaaga ttttcccggg tttactcttc
      601 ccgtcaaaaa agtcaaaacg aggcaattgt acctcctcgc cggcaattaa acggtcggta
      661 caacgatcca tcaaatccaa atttaaatta tcaatcgatt caaagtcata atttccgtcg
      721 ggagtccgtg ggcttcgttc ccgatcgaca aaaaaatcat ccatttcgat cggaatcggt
      781 gagattccca aagctaaaag ttcgatcgat aacctcttag aaaatgtggt ttttccggag
      841 gaagacggtc ccgcaatcaa gaccaccttg acttcatttt tacgctcgac gatatctctc
      901 gcaatttgga caaacttttg atcatgcaga gcttcgctga ccaaaatcac ttcctgaata
      961 cggtcacttt cgatagcttg attcagggac gcgatcgagt caatcccaag ccgttgcaac
     1021 caatccccat atcgacgaaa ttcagacatc atcttcgagg attcatccaa tttttgaatc
     1081 acatttgatt taccgtcgct gggataacgc aggaaaaatt cattgggtct gcccgccggt
     1141 tcaattccga aaaccttcag gtaacctgtc gaagggacca tgtacccgta atgataatca
     1201 acgaaatccc ccaacttata ggctgtcagg taatcttttt tccgatattt caataaatcg
     1261 gctttttcct gttgatgttt cgatgtaaaa agggcttgag cttcttcaat cgtcagcttt
     1321 acccgcagaa tgggtaaatt tgcatcgata gtcgcctgca tttccgtctg aagcttttta
     1381 taaaacccct tcggatcctg cagttccggt cctttgattc tgcaaaacaa gccgcctgaa
     1441 tgaatggaat gatctaccat taattcatag tcaggcatca ttctttcgaa tgttgccgag
     1501 agtaaataga caagcgatct gcgatagata tttttgccat ccgattgagc ggttgtaacc
     1561 ggttcgatat tcgcattact gattaacggg taactgagct ctcgtaattc tttattgaca
     1621 atcgccgcaa cgagcggaaa ttccaattcc gaaacaatgg gctgcagaaa ttcttccact
     1681 gttccgccgc gcggtccttc aatcaatcgt ccgtcaggca actctgcttg tataatatta
     1741 gtaggttgaa tgattttaaa tctctgatcc atgatgcctc attttcttat tgattatatt
     1801 cggaatcggt tgaatggatt gattttacag gattaaaata tcgatgacaa agatgacaat
     1861 ttttattatc ctgaccgttc ttctgttgat ccgaatcatc atcggtcttt gggcaaaaaa
     1921 acggattttt tcgttgacat ccatcccccg caacgaatac ggtatcgttt ttggcgcagg
     1981 cttagaaaaa acagggcttc ccagcaacgt tctgatggaa aggattcaaa ctgccgcaat
     2041 gctttatcga gccggaaaga tcagaaaagt tttgttgacg ggggataacc attcgcttta
     2101 ttataatgaa ccgcaagcca tgaaaatcgc ggcaatgact ttgggcatct ccgaaaaaga
     2161 tattttgatt gatccgaacg gacaccgttc gttcgattca tgccgaaatg cgatatgcga
     2221 ctttaatatt catcaagctg ttttaatcac gcaacctttt catttgccgc gtgttctttg
     2281 gctggcggtc agcgtcggaa tcgatgcgat cggtatgccg gtagacctga aaagacatcg
     2341 gattgatgat gaaatctggt ggcggttccg ggagctgttt gcttcggtcc gggcaattat
     2401 tgatggtttc atttataaaa tatgctattc tgtaaacgag aaaagaatga atgacagaaa
     2461 aggagaaata aaatgagtaa agacatcgga tttttcactt tgaataataa tgtcagaatt
     2521 cctaaaatag gttttggtac ctataaagcg gccgaaaacg gagatgaatc cgttattaag
     2581 gaagcgatcc gtcagggtta tacattattt gataccgctt cattctatga aaacgaggcc
     2641 attgtcggtt ctgctgttaa aaaatccggc ttgccaagag atcatttctt cctgacttcg
     2701 aaactgtgga aaactcagat gggctatcaa aatacgttgc aggcttttaa tgattcttgt
     2761 aaacagttga aaaccgatta tctggatctg tatttaattc attggccgcg gcctgacctc
     2821 gtatgtgatt ggaaaaagat tgttgtggaa acttggaagg ctctcgaaga attatatgat
     2881 gagaaaaaag tgcgggcaat cggagtcagt aactttctgc cgcatcattt ggatgtgatt
     2941 ttaaattcct gtaaaataat gccggcagtc gatcaaattg aatttcatcc gggatacacc
     3001 caaacggaga cggtcaattt ctgccggtcg ttacgaatcc aggtagaagc atggagtccg
     3061 atcgggcgag cccggctgat agataacccg ctgctgaaag aaattgcggc aaaatataat
     3121 aaatccgtcc cgcaactttg catccgtttt agtttgcaaa ataatgttct gccgcttccg
     3181 aaagcctcct ctcccgaacg aatggctcag aatctggacg ttttcgattt tgagatttcg
     3241 gaagaagata tggaaaaaat aatggcgatg cccgttacgg ggtggtcggg agaacatccg
     3301 gacgccgaac cggttgcgat ttaatcattc gatcaagaga tgattttacc tgccagtgaa
     3361 atgccgtcaa aatcagtcac ctctacgttg ctgatttggt tcaataagtt ttgactgccc
     3421 aaacatttca ccggcaggta attttcactg aatcccttca atcggcaatt ttctttgttg
     3481 atcggttcgg cagactccca aagcacattt gtggttgtcc cgatcatttt catgccgaaa
     3541 gattttcttt gttcatccaa ttgttcccgc aatattttcg atcgttcccg tttcacatct
     3601 ttgggaattc ttccgtctaa tttcgccgct gcggttcccg gtctgacaga ataggtaaac
     3661 acatgcccgc cgctgaaagc cgccgtccgg acaaaatcaa gcgtccgttg aaagtcttgg
     3721 tcggtttctc cggggaaacc gacgatgata tcggtagtca cggaaaaaag gggattgatt
     3781 tgtcttacac attgaatcag ccgtaaaaaa tcaggcaaac tgtttcggcg tgccattcgt
     3841 ttcagtaccg cttccgagcc cgattgcaac ggcaaatgga gatgaggaca caagcgttta
     3901 ttttcccata gacttataaa tgcttcatcc agattccatg attccaaaga ggataaccga
     3961 attcgcggaa tcgaagtttc cgccagcagt cgattcaata aatctcgtaa cccttgtggc
     4021 ggattgagat ccattcccca tgctccgaga ttcacaccgg tcaaaacaat ttcttttccg
     4081 ccgccgtttt ccgccgcacg aatatcctgt aaaatcgttt cagccgccac actacgcgat
     4141 ttgcctcgag ccaatcgcgt aaggcagaat gtgcaataat tgtcgcatcc gtcctgaact
     4201 tttataaaag cccggttccg aaaatgctca ccgggtaaga tttcacgctt aatcgaaaca
     4261 ggatttataa acggttccgg tattccggtg acaattgagg caatctgttc tttttcgtga
     4321 tttcgaatga cctgtgttac tcccggcatt ttcgaggcgg cttccggttc cgtttccgac
     4381 caacaaccgg tgacgattac ttgagtatgt ccggatcgag cggcatttcg aatttttcct
     4441 ctcgaatccg aagccgcttc tttcgtcacc gaacaggtat tgataatcgc taaatcggct
     4501 tcgtcggcac tgtccgcaat ttcgtaaccg tattgtttga attttgccgc aaattgttcc
     4561 aattcggctt gattcaagcg acagccgaca gaatccaaaa aaactttcaa tgacttccct
     4621 cggaaccttt gcccagctca attgttcgtt cgtagcacgc ccacattgct tcggcaatcg
     4681 cattgcgaaa acccaatttt tccaattcga aaacggcttc ggctgtcgtc ccgccgggtg
     4741 aggtgacttc atttcgtaat acagccgggt gctcttttcg catggcaaga taatcggtcg
     4801 accctttgac ggttcgaata acgatttcag tcgccaaagg tcgcgataac cccagatgaa
     4861 ccgccgtatc gatcatcgct tcgataaaca gaaatacata agccggaccc gaacccgaaa
     4921 ccgctgttat ttgatccaat tggccttcgg atttcaactc aaaggcatat cccgtacaag
     4981 cgaggatttc ttgtgccaga ttccgttgat cggcagccac ttcacgagac gcaaaccatc
     5041 cggaaacccc ttcgccgatt tgtgccggcg tgttgggcat cacacggaca acccgctcgt
     5101 gatccagtcc cttttggaac tgttcaatcg ggacacccgc aacaatggat atcaccagcg
     5161 catccggatg aattttattt ttcaattcgc caaacacttc ggaaagaaat tgcggtttta
     5221 ccgcgatcaa aatcacatcc gcgtcacgga tcgcgtctgt cccgctgtct gaaggattga
     5281 ctcccaattc ctcataagca gaccgcattt tgtcggcatc ggtatcataa acagaaatat
     5341 tttgcggaga gatcagcctt tttttcaaga tacctgtcaa tattgctttt cccatcaccc
     5401 cgataccgat aatcgccatt ttttcggaat tcatttttta tttctccttt tgccaccggc
     5461 tgtaaaccgc cattgacagt aacctacccc cggacttgtc ctttaaacat acttctccgg
     5521 cggaaacact gccacccatc cctttcatta cttcattgac cgtattataa actgttacgg
     5581 aagaagcctt gacggcataa gcagtaacca acaaaaacaa agcctcggaa tccaaaatat
     5641 ttcgacaggc ttgaatcaat tccggaaagt tcttataaaa ttcccaaact tcccctttgg
     5701 gtcctcgccc gaatttcggc ggatccaaaa tgattccccg gtaaaaggaa ccgcgccgtg
     5761 cttctctctg aacaaacttt agcccgtcat cgataatcca acgaatgggt gtttgttcca
     5821 aagacgacaa tttttgattt tcctttgccc aatccacagc ttttttggag gcgtccagat
     5881 gagttatttc cgcccccgcc tcggctgcgg ccatggtaat cgcaccggta tagccaaaga
     5941 gatttaatac tttaaccggt ttctccgaat ttcggattag ggaagacacc caatcccatt
     6001 ggcaggcttg ttcgggaaac acaccaacct gtttggagtt ggataacatc agctgaaatt
     6061 tgagattttt ataaccgata ttccaaggat gaatatcttt ctcataaaaa atccaatggc
     6121 cgccgttttc ctcaggagaa ggtttaaatt cagcagaagc tttattccat tccgtttcgg
     6181 gcagtaacgg gctccacata gcttccgctt ccgggcgaat cagaatatat ctgccgaatc
     6241 tttccagttt tcgtcctgct ccgctgtcca aaagcatata atctttccaa tccggagatt
     6301 ccaaaagctt cacttcgggt aaaaatttat tattcatagc gtcttcaagt atacttagat
     6361 ttgtcggatt tttgaaccga aacaggaatt tgcggaatga tagggaaaaa atccatttga
     6421 attcaaaata attcgaaatc gggaccggca tctgataaac acggaaagaa aaggggttca
     6481 tgacgaaaat tatcgaagtc gacaaactgt caaaatctta cggccccgtc aaagctgtac
     6541 aagatatcag tttttatgtt gaaaaagggg atctttttgc ttttctcggg ccgaacggtg
     6601 ccggaaaatc aacgacaatt gatatcatta caaccgttct catgcccgat aagggaacag
     6661 taatcgtgga tggtttggta ctgggaaaag aggatgataa gatccggtct tcgatcggag
     6721 tcgtttatca ggataatctt ttggatcgtt tattgacggt ggaagaaaat ctcttcttcc
     6781 gagccggttt ctacggattc aataaagatc ggatcagaga gtccgtaaag aatgccattt
     6841 taactaccgg aattcaagat tatgcgaaac aacgttatgg gaaaatttcc ggaggtcagc
     6901 gacgcagagc ggatatcaca cgcgctctga ttaacacacc ccaaattctg tttctggatg
     6961 aaccgaccac cgggctggat ccgcaaaccc gtaagaattt atgggaagtt atccgaaaat
     7021 tacaacgcga ccgcgaaatg acaatctttt taacaacgca ttatatggag gaagcggctg
     7081 aagcggatta tgtcgtcgtg attgacgagg gaaagattgc cgctcgcgga actccgtttg
     7141 aattaaaaga gaagtatacg agtgatcttc ttaaactatc ctttaaagac gaaataaaag
     7201 ggttgtccga cctggcagat tggggaataa catacacaaa agcaggggat cagtttatta
     7261 tccgattacc gcatactaca gatgcgcttc ccattttaga ccgctgcaga gaaaacctga
     7321 acggattcga agttctgaac gggacaatgg acgatgcatt tatcggaata accggaaagg
     7381 aaattcggga atgatcgcac ttgcatccag aaatatcaaa ttgtttttcc gtgataaggc
     7441 aatggttttc ttttcgttgc tatcggtctt tatcattatc ggtctttatg tgttgttcct
     7501 cggcaatgtc tggaccagcg aatttcccaa tcaagaatat gcaaaattca tcatgaacag
     7561 ttggattatg gccggattga tatccatcac agggtttacg acagccgtcg gcgccttcgg
     7621 aattatgatc caagatcgtg aactaaatat agaaaaggat tttctttccg ctccggtcaa
     7681 acgcagcagc ttggcagcgg gttatctgtt aagcgcctac ggaatcagtg taatcatgag
     7741 tctgattgct tttattctcg gggaaatctt tattcttgcc gaaggcggag aacttttagg
     7801 catagttgcg atattcaaaa taatcgggat cattttgatt aactcattca tgagtgtttc
     7861 aatcattcag gtcattatcg gattcattca tacgggaaat gccttttctg cattcagcac
     7921 aatcatcggt acattgatcg gttttttgac gggaatttat ctcccgatcg gacagcttcc
     7981 gaccggtgta cagaccctta ttcgcatttt tcccgtttcc cattccgctt cacttgcccg
     8041 tcaaattttt atggatgttc ctctgaaaat cggtttcaac ggtgtccccg aagcagccgt
     8101 caatggtttc aaagagttga tgggagtcac tttttttatc ggaagcaaag aaatcacacc
     8161 atggatgagt ttatgctata tgctgcttat cggagccctt ttctttgcga ttgccgttat
     8221 gaatctctca aaaagaaaac tggaataagc aaagaattcc tctctgatcg taaggagctg
     8281 agataatttc tctcctgcgg attcgggcgg ttcttttatt caaaggaaaa tacacctcgc
     8341 agtactgaga acatttccgg cgatggatta attttcggat tgagaaaata tttgatccga
     8401 attgaaccga ttaaaacaat ctctcaagcg atttctttaa gtcgaatttc actcgatctt
     8461 tatcgatcga taaaaattca cctttttcat aaagagttct tccgtctacc attgtcataa
     8521 ttacatccgc agcttgtgcg gaatagacaa tcaaggaagg ataatcatga atcggcatca
     8581 gatggggttt gtcaagatca atcactgcta aatcggcttt ctttcccact tccaattctc
     8641 cgcaattcga acgtccttgt gcagctgcac cgttcaaagt cgccatcgta aggacttcat
     8701 ttggagacaa aacggtcgga tcctgagaaa aaccgcaatg aattaatgcg gttaaagcca
     8761 tttcttcgat catattttgg ttgttgttac tggcggaaga atccgttccg atcgttatac
     8821 gcagtccttt ttctttcatc ttccgaatcg gcataacacc gcttcccaat ttcatattac
     8881 tggaaggatt gtgaacaacc gtcacatctt tttccttcag taagagaata tcttccggct
     8941 ccaccatcac acaatgtgcg gcgatcgtcg gattgtcaaa cgttcccaga tcatagaacc
     9001 aggcagccgg agtttttcca taacgtttct tgcattcttc atgttctttt ttggtttcgg
     9061 aaagatgaat atgcatccga ccgttttttc ccctacatat ttccgaatac tcgcggacga
     9121 tctcgacatt gttggtatac tcggcatgaa tcgcaaaatc cgtcataatt cttccgtccg
     9181 cagaaccatt atattcatca aagaattcaa ctgacagtcg cataatctct ttatttttat
     9241 ccgaatcgga agccataacc gccataatcg gaaagctcag atttgctttc ataccggatt
     9301 gaatcacatc ctcgatataa tggttcggat aatggtacat atcagaaaaa gagaccactc
     9361 cggaagccaa catttccatc atttccaacc tgaccccgac gctcttatcc gccggcgtca
     9421 ttttttgttc gatcggaagc atggcttcaa ataaccaacg gtccaacggt aaattagtcc
     9481 ctaacccacg caaagcagcc atgggggtat gagtgtgcat gttgtataag cccggaataa
     9541 tcagacaatt gtaaaaattt ctctcaacat catattcttc gatcggtttt gtctcgccga
     9601 tataagagat tatgtcattt gtaatcccca aataaccatc ccgaatgaca ccctggctat
     9661 attttggtgt taagatatca acattcgaaa ataaggtttt catgattttt ctctcccgaa
     9721 tttttccgtt caacttgctt tgttgattaa aaagtttaag cagttcattt acacgatgga
     9781 aatcaaaaca aaaagtagcg agttattgga tatatcatcc catcgctaac cgaatttctg
     9841 aaattatacc tgtgaaaacg gaaaacaaat gccgaaagga ggtattttta aaattatttt
     9901 atataaattt aattattgat tatcgtacta tattttagta tatgtttgat ctaataacca
     9961 tgaatccatt gagaaatttt tcattatgtg agaattactc atatatttaa tattttattt
    10021 tttattatta ctttcaactt aattcgatag caatttgtac tatatttcac ataattaccc
    10081 aaatttaatt gacgtttccc tattattaac ctatcattta attattagcg ttggctataa
    10141 ataaatagaa tataggttga ttgttcgtaa tagaaggaga ttttatggaa aaaaagactc
    10201 taatagttaa tggaatccgc caagtcgtta ttcataatcc ggatgcgatg ttagcggatg
    10261 tgcttcgaaa ccagctgcat gttctgggag taaaaaaagg ttgcggtcag ggacactgtg
    10321 gcgcctgttc aatcatattg aacggcaaac ttgttttatc ttgtatccat aaaatgtccc
    10381 gaattccgga tgaatcaacc atattgactg ttgaagggat cggtacaccc ggacatctgc
    10441 acccaattca aacagcaatg aaattccaca gcgctgcgca gtgcggtttt tgtactccgg
    10501 gttttgtgat ttcttcctat gcattgctac aggaaaatcc gaatccgact cgacaacaaa
    10561 ttcgggattg gtttacagaa catcataatg tttgtcggtg caacggctat aaaccttata
    10621 ttgattcagt gatcgatgcc gcagccgtat tgcgcggaga aaaaagtatt gaagaaataa
    10681 catataaaga acccaaagac aataaggttt ggggaactcg tcgtcctcgt ccgtccttgg
    10741 atgcaaaagt gaccggcaca tgggatttcg gtgcggatgt ggcagcaaaa atgccttcga
    10801 atacccttca ttgtgccttg gttcagtcac aggtacctca tgcgaatatt atctctgttg
    10861 acacttccga agctgaaaaa gcggaaggcg ttttcaaagt ggtaacggcg aaagatgtca
    10921 aaggtcgaaa ccaaattacc ggcctgatca ccttcccgac aaataaagga gacggatggg
    10981 atcgtccgat tctctgcgat aaaaaagttt tccaaatcgg agacgcgatc gcgattgtct
    11041 gtgcagatac ccaggaacag gcggatgccg cggcaaaact ggttaaagtc gaattggaaa
    11101 ttttgccggc ttacatgaac gctccggccg caatggcaga agatgccatt gaaattcatc
    11161 cggggactcc gaatatttat tttgaacaga agaatgtaaa aggaaacgat accaaaccta
    11221 tttttgaaaa agctgcttat acggtcgaag ggaattatta cctgcaacgc cagccgcatc
    11281 tgacggtgga accggacaat gggtttgctt acatagacga agacggcaat ctgactattc
    11341 attcaaaatc catcgggatt catctccatg cggcaatgat tgcggaaggg atgggtgttc
    11401 cgctggataa actgcgaatt gtagaaaata atgccggcgg cactttcgga tataagttca
    11461 gcccgacaat ggaggctctg gtcggagtcg cttgtctggc tacacaacgt ccggtcgttt
    11521 tgatctacaa ctattatcaa catatgattt ataccggaaa acgctctccc atgttcctga
    11581 aatgcagaat ggcagccgat gaaaacggca aattattagc catggaaggg gattggtctg
    11641 ttgatcatgg tccttattcc gaattcggtg atctgctgac cttgcgcgtc agccaattta
    11701 tgggcgccgg atataatatt ccgaacatcc gaagtatcgg aagaacagtt tgtaccaatc
    11761 atatatgggg ttccgccttc cgcgcttacg gctcgcctca agcttttttg gcttcggaat
    11821 cttgtatcga tgaactcgcc gaaaaaatgg ggatcgatcc gttcgaactg cgttatcgaa
    11881 atatttatcg tgaaggatca actacgccga ccggtcaaaa gcccgacagt ttatgcttcg
    11941 aagagatgtt cgatacgatg agaccgaaat atgaagcagc taaagctaaa gccaaagccg
    12001 aatcgacacc ggaatataag aaaggcgtcg gtatttcatt gggtgtctac ggttgcggat
    12061 tggacggggc cgattcttcg gaagccattg ctgaattatc cccggacgga aaagtcatta
    12121 tctattcttc ctgggaagag catgggcagg gaaatgatgc gggtgccttg ggtaccgctc
    12181 atgaagccct tcggaaagca ggcttcaaac ccgaacagat taaattggta ttcaacgaca
    12241 ccaaaataac ccctaattcg ggacctgccg gcggttcacg ctcccagacc atgaccggaa
    12301 atgccattcg aatcgcctgc gaaaatctac ttgaccaaat gaggaaggcg gatggcagtt
    12361 atcgtactta tgatgaaatg aaagcggacg gcaaggaaac caaagtaacc ggaaaatttg
    12421 tcatgtccga agccaccagc tgcgacgaaa acggtcaagg tcgccccttt atggtttacc
    12481 agtacggttt attcatggcg gaagtgacgg tagaaatagc aaccggaaaa accaaagtcg
    12541 ataaatttac ctgcttcggc gattgcggtg tcatcaacaa ccgtgcgatt gttgaaggcc
    12601 aaatgctggg tgggtgtgct cagggaatcg gcttggcgct gtcggaagac attgaagact
    12661 ataacaagca tactaccatg cgcggtgcag gaattccgta ctgcaacgat atccccgatg
    12721 agtgggatat cgaaatgttc gaaaatccgc gcgaatatgg tcctttcggc gccggcggag
    12781 ttggtgaact tccgctgacc gcttcccatg ttgcggttat caacgctata aataatgcct
    12841 gcggtgtgcg aattcgccac cttccggctt acccggaaaa ggtgttggaa gggttgaaag
    12901 cgctgaacaa gtaaaaagtc tgattttatg caaaaggctg tccttctcct ccaacagggc
    12961 agccttttat ttttgagaag atatgattca tttcaatttt tcaagctaca ataacaatac
    13021 caaggagtaa aaatggatca gcaattcatg cgcacaatgg aatccatatg catccagaat
    13081 gagccgcctg cttgtgtgac cgcttgtcct ttacatgtcg acagccggac gatgttaaaa
    13141 ctggttgctt ccggaaaatt tgaggacgca tattcactct atcagaaaaa tgtcccgttt
    13201 ccttcgattc taagcaaaat ttgtaattcc ccttgccttt caaaatgccg tctttctgaa
    13261 atcggtgagg gcatacaaat ccgtccaatc gaagcttttc tgaatcagta tgccgatccg
    13321 tcttttttac caaaggtatt gccggaaaaa cgccagaaag cgggaatttt cggatcggaa
    13381 ttggacagct tatccgccgc atatgattta cgcaggaaag gttaccaagt gacgattctg
    13441 gatgcggatg ccgttttagc caaaaatttg cgtgcgttta acgaagaagt tttaccttcc
    13501 gttgaaatag aaaatgcaat tcatttgctc gaaaaaatcg gtgtaaaaat acaaaccggt
    13561 ttcgaaatgc cccgttttga agaattatct cgtaatgatt cgggcttttt ccgaatcggc
    13621 gattcggaat ttcaatgcat ttatcttccc cctttgtccg gagagctttc aattgattcg
    13681 ataacccgta tgacggttac cgaagggatc ttcagcggaa gcgcatcggc cggtcactgg
    13741 gtcgatcaaa tgtccgacgg gcggagagcg gcgatttcta tggatcgtta tctgcaaaaa
    13801 gtatccttga cggcttccag agaaaacgaa ggagcctatg aatgccgcct gttcacgtct
    13861 ctaaaaggca ttcaaccttc cgaatcgttc atttctacgg acataaaaag aaccggccag
    13921 gcggatatca taaaagaagc cgcccgttgt atccagtgtc aatgtttgga atgcgcaaaa
    13981 ggatgtgaat ttattcgaca ttaccaagat tcgccccgaa cttatgtcag gcagatttat
    14041 aacaatattt ctatttgtac cggattgcgc cagaaaaata aaatgataaa ctcttgctcg
    14101 atgtgcggcc aatgtgaagc catatgcccg aaccatattc cctttcaaaa agtcgtccga
    14161 gaggctcgtg aaacaatggt ggcaacgaag aaaatgccgc cgtccgcttt cgactttgcg
    14221 ctgcgagata tggctttttc aaacagtgag aaatttcggc tcgcgaaagt tcccgtcgat
    14281 tcaaaagctt gcaaatatgc atttttcccc ggctgccaat tgccggcatc gaatccttcc
    14341 gcaatcgaaa ccatattttc atggcttcgg gatgaaatga atgaaccggt cggcattctt
    14401 ttacgctgtt gcggggcaat cgcggattgg gcaggtgaaa aagaaaaatt taatcaagct
    14461 cgttcggagt tgttagcgga ttgggaatcg ttggggaaac ccgaattaat tgtttcttgt
    14521 cccacctgtt tgaaaatgtt tcgcgagaaa tatccggaga taaacgtccg ctccatctgg
    14581 ccattaatgg cagaatccga ttctttattc aacggaaaaa gtcggctgaa ttcaattaaa
    14641 atggcgatcc atgacccgtg cgcttctcgc gaccaaccgg acatgcaaga agctgtccga
    14701 tcccttgcga tgaaattagg gattgaattg gaagagctgc cctataatcg tgagttggca
    14761 acctgttgca gctacggcgg aaacgtctgg aattcgaacc cttctttaag caaggacacg
    14821 gttgaagctc gtatttctca aaatcaatcg gattatttga cttattgcat aatgtgtcgt
    14881 gatttctttt tgagatccgg aaaaccggca tatcatattt tggatatttt attcgatccg
    14941 aaattaataa tcgaacatcg gttaccgatt cgacccgatt attccatgcg acatgaaaat
    15001 cgaacccgag taaaaagaca tttattaaaa caatattgga gggaagaaat gcctgaagaa
    15061 gccccttatg aagatataca acttattctc aatgacccaa tccgtaaact gatggaagat
    15121 cggatgattc tcgaagaaga tatccgtcaa gtgctttacc atgcaaaaga atcggggagg
    15181 gcaatgatta atcaggaaac cggtcattat ttggcgcact ttaaaccctc ctgtgtcacc
    15241 tattgggtgg aatacctccc taaagaagac ggctttgaaa ttttcaatgt ctattcacat
    15301 cgaatgtttg ttgaggaggg aaatatctga tggcacaata tgtcaatgaa gaagatttta
    15361 ttcactggcg ctgcgcaaaa tgcaacagtg aactcgaact gagaaaagtt gttgtctctt
    15421 atttggacgg ttcttttccg gttgaattac cggtttgtaa agtctgcgga caggtcttca
    15481 tcccggaatc cttggcaaca cagaagatgt tggagattga acaacaaatg gaggataaat
    15541 aattccggga agctgttttc ggatgaatcc cgctccggga ggaatcgccc tgaccgaaaa
    15601 gttgctcaac ctcagccttc tccaaaaagg ctcgatgatt ttagatgtcg gttgcggaga
    15661 cggagatgcc tgtcgattcc tatcatcgga aggatatcga gcgatcggtt tggacatcgt
    15721 tcgacattct tccggaaaaa tgaattcgga aagaattcaa ggacaaatca acgctattcc
    15781 catattagag gaaaccgtcg atgcggtttt aatggaatgc gtattgagta tcttgccgac
    15841 cgatgatggt tcattaacgg aaataatacg agtgttgaaa cccggcggaa ttcttttgtt
    15901 cagtgatctc tatatgcgat accctgatga ggaatcagaa aaaaatcgaa tcgattccga
    15961 aagcgattat ccgaaaatcc gtctcgcaga cgaattattg aatatctttg gcgaatcagg
    16021 gctttcagtc cgttactggg aagatcagtc tgcggttctg cagagtttac gacttaaatt
    16081 tttatgggaa ggaagtgctt ccgaaaacaa gttcccatat aaaatgaaga atatcggata
    16141 tatctgtttg atagccgaac gtgtatcagc aagcgggtaa tgataaaagg agaaaaaaat
    16201 ggatgattcg gatgaaatca tagaaatggg gaaacaaggt tttacttgca gccaaatgtt
    16261 aatgttgaaa gccttggaac tccaaggtaa ggataatccg gatttggtcc gttctatgac
    16321 gggctttgcc ggtggattag gctggacggg tgatatatgc ggtgtcttga ccggtgcaac
    16381 cgctgttctt agcttatatg cgggaaaagg tcaagccgat gatgaggatg acccgcgctt
    16441 aatgtttatg attgaagatt tggttcactg gtttcatgac aagtatggaa aaatttacgg
    16501 aggaatccgt tgtaccgata ttttgggtcc cgacgccgga aaaattgata tgcagcgctg
    16561 ttcggaaata attcaagaca ctttccaata tattaaagaa ctattggtaa acaacggatt
    16621 tgatctctcg ggagaaagtt tggatggatg attaccgtga actctcacaa accttgagcg
    16681 tttgcccgat ttgcttggag aaaatccccg caagttacac cgttcatccg gatggtcatg
    16741 tttatttgga taaagattgt ccgagtcatg gacatttttc gacaatcgtt tggcgggata
    16801 atccggattt cgaatcatgg acgatgcgca gtacccctta tccaaccgaa cacccatcca
    16861 ctttgatcat tcggggatgt ccctatgatt gcgggctctg cccggatcac caacaaaggc
    16921 catgttgtgt attgttagaa gtgaccagtc gctgcaacct gggatgcccg gtttgttatg
    16981 cttcctcgga tgaatattcg gatcttgatc cgtctttgga gacaatacac gggtggtttc
    17041 aaatgcttct tgcagcggga ggtccgttca atatccaact ttccggcgga gaacctacag
    17101 ttcgcgacga tttaccggaa attattcgta tgggtaaaga aatgggattc ggcttttttc
    17161 agctgaatac gaacggaatc cgtatcgcac aggaaaaagg ttatttggaa tcattatcgg
    17221 aagcgggatt gtctgtcgta tatttgcagt ttgacgggac tgaggatacg atctatcgta
    17281 aaatccgcgg acaggattta taccaaacaa aattaaaagt aatggaacga tgtaaagaag
    17341 tcggaatcgg tctcgttctg gtccctacgg taataccggg agtcaatgat gagaatttgg
    17401 gagagattat ccgcttagcc gtttccaatc tccctatcgt tcgatgtgtg cattttcaac
    17461 cgatcagtta tatgggaagg tatgaaaaaa cgcccgaaaa cgcagaccgg atcacgctcc
    17521 ccgaactcat gcggaaaatc gaagagcaga cgaatggatt gattcatatc gaaaatttta
    17581 aaccgtgcag cggtcaacat ccgaaatgcg gatttcaagc caattttttg catacggaag
    17641 acggctcttt gatacctctg tcaaagttta atccggatga aatttcaagc ggttgctgca
    17701 gtctttcggg agaccgacaa aattcaagtc aaaaagctca aaatttcgtc gccaaaactt
    17761 ggacacttcg gaatcctgaa gagggaaagc aatccgatta caaaatcgtc tcagactggg
    17821 attctttctt agaaaaccgg caaaaaaatc atttcacaat ttcagcgatg gcttttcaag
    17881 atgcgtggaa tcttgacttg gaacgtctga agcgctgcta tatcatgatc gtttcaccgg
    17941 atagccgcct tattcctttc tgcgcttata acctcagcaa tcgagacgga gagacacttt
    18001 accgcaaatg acggagccta tccatgttcc gaagatgatc actcccgttc aactggaaat
    18061 tgaacgaaaa ctcggcattc cggaccactc cttgacgctc gaaaagttaa aaaaatggca
    18121 gttagatcga ctgaatgaca ctttggcgtt ctgtatgaat catagtccct tttacaagga
    18181 gaaactggct gatttttcga attcgattcc attaagaagt cttgaagaat tacaacagct
    18241 tccgtttacg acttcggatg acatttcgga aaaaggacac cgcatggttt gtacttctca
    18301 aagtcaaata gaacgaattg tgacattgaa cacttcaggt acaaccggta ccgcgaaacg
    18361 aatttatttc accaaagatg atcaacaatt gacggttgat ttttttgcct atggaatgtc
    18421 gactttaatt tcttcacatt caaaagtttt agttttactc ccctgtcatc cggcgggaag
    18481 cgtaggtgat ctgttaagtc gggcaatcag aaaaatcggt gctcaacctt attcaatagg
    18541 acctttcaac aatccggatc caatcaatga tttaatcgct tcggaaaaat tggacgcatt
    18601 ggttggcgcg cccaagcaag tttatacatt ggttcaatct tggaaatcat ccaatagagg
    18661 gagttgccca attcaagcct tacttttatc cacggattat gcgtctccga tcttgaaaaa
    18721 atcgatcgag tccgcttgga attgtgacgt tttggaacat tacggcatga cagaagcggg
    18781 tctcggcgtg ggagtggatt gttatctgaa gcgcggttac catctgcggg aagcggatct
    18841 ttatttcgag ctgatcgata aagacggaaa tcctgttgct gaaggtgagc ccggtgaagt
    18901 agtgttcagt acattgaccc gtcatggaat gcctttgata cgctatcgga ccaaggatat
    18961 cagccaaatt atcccgggag aatgcccatg cggtactgtt ctgagtactt ataaatggat
    19021 ttcaaaacgg atgcaaaacg aggatagtct ttgtacgatc gccgaattgg ataatattct
    19081 ttttcaaatt ccggaaataa atggatttga cgccgcggca aaaaaagaat cccttaattt
    19141 gaaaatatat tctgacaagc aaaataaaga ggaatcaata aacaaaatca gtgaatcaat
    19201 cttgaatacc ttccccggcg ttaataaaat tgaaattgag ttactggccg gttttccctc
    19261 ttctgcgttt tcattgcaga agaaaaaaat agaatacatt tcttgatggt cacttttgag
    19321 attatctttt cggtataaga tcaaaatgct gcctgcaatt aagccggatt tctaaatatc
    19381 ttgcgctttt ttttcgagaa aggatcatcc gattccatta accgaaaaat taatttgggc
    19441 acttttcgaa agaatatggc acgttcattc aatgaaataa ttgaaagaat caaaatgtcg
    19501 tgttaagatt aatttaagta tgagacagat atttctaacc cagaccgaga cgattcttct
    19561 tgatattgtt atttgggtaa ttattcatct ttcgataggt tattggacat ctcgtatccc
    19621 cgtaaatgtt tttaatcctg aatcttggct tttccgcaca agaaaatggg aaaaagacgg
    19681 catgttttat gagaaatatt ttcgagtaaa aacttggaaa gaccatctgc cttccggcgc
    19741 tgcgctttat gaagatgcct atgaagtgaa gcatatcgga gattattcga cggaaaatct
    19801 gcgtacctgg atccttgaaa gttgtcgttc ggaattctgt cactatctca tgatcatccc
    19861 cggctttttc ttttttcttt ggaataccgt taatgtcgga tggattatgg tagcttatgc
    19921 atttgcgaac aatataattc ccattattat gcagcgctac aatcgtccgc gggtgaagaa
    19981 acttttacga tatatggaac gttctaaaaa atattcccga caacaaaccg tcagcagcgg
    20041 ggaagccctc gccatataaa tttttcgtta ttctgcgatg gctaaagcaa ttttcaccat
    20101 atcattaaat gaattttgac gttcttcggc acttgccgcc tcaccgcttt caagcaaatc
    20161 cgatacggtg cagatagtta aagccttctt gccggcgcgt gccgcattca tatataaggc
    20221 tgccgattcc atttccacac ctaagacacc catctttcgc cattggagag tctccgcaat
    20281 gccgccttcc cggtaaaaaa catcagagga taggatattt ccgacttgaa atgaaatatt
    20341 atttttttca cattcttcgg cagcttgtcg taataatcgg aaatccgcaa tcggagcaaa
    20401 tttgcccggg atatggtagc ttttaaaata attcgaatcc gtacaagcgc cttgtgcaag
    20461 aatgatatcg tacagtttta aatccggcgc aatggcacct gccgatccga cccgaataat
    20521 ggaactgacg ttgaaaaaat gataaagctc ataggaatag agtcctatgg aaggcattcc
    20581 catcccgctg cccataaccg aaacacgctt cccatggtaa taacccgtat atccgtttgc
    20641 tccgcggata tggtttacca attccgcatc ggttaaataa gtctctgcaa tcatttttgc
    20701 ccgcagagga tcaccgggta acaaaacggt ttttgcaaaa tcctcgggac tgcattcaat
    20761 atggggtgta agattagctt ttttcattga acctccggcg tcgaaatgag agtaaactca
    20821 ctggtataat catagtgaaa attataattt agctgacaaa gtttcaaata gatcgcggca
    20881 atttaagttc ctgttttctg agaactcacg gctaaaaagc aaagaacaga atttttcact
    20941 tgagaccgat tttaccaaat aaattcacat tgacagatac tgattctcgg aacgaatctc
    21001 ctccgtttct tttatgacta atcgaaatat ctgatttcaa atctttattt cagctaaaac
    21061 atgtatttaa taaggataaa atttttatga atattttaat ttttaattgt ggcagttcat
    21121 cacaagcatt caaagtttac caagtctcgg aaaaagatca accgaaaata atcgcatccg
    21181 gaaaagcaaa aaatgtcgct acgcaaacga aagcggattc attcatcgaa tatcggatca
    21241 atgataaaca gaggaaagaa ataattagcc ttccgtctca tcgtattgcc gccgacgtta
    21301 tattaacttt attggaacga gagaatgtca cgattgatgg catagggcat cgttttgttc
    21361 atggcgggcc gcgtttcaat cagagtgtta aaatcgatga aaatgttttt tatgaattaa
    21421 aaaactcctt ccgttttgcc ccgattcaca atccaaattc cttcagtgtg atcgaagttt
    21481 gcaaagcaag gctgccggag atcccacaat atgctgtctt tgatacagcg tttcatgcaa
    21541 aattacccaa agaatcccaa gtctatgcac ttcccaaaaa acttgccggt gaatatggct
    21601 ttcgtaaata tggtttccac ggtctgtctt atcaaaatat cctttcgaaa atgccggatt
    21661 tgctcggaaa accgatcgaa cagttgaatc tggtcatgtg tcatttggga accggcggat
    21721 ccagcgtaac ggcaatcaag aatggattgc cttatgatac ttcgatggga tattctccct
    21781 tgcaaggact gatcatgtca actcgttgcg gcgatctgga tccggaaatt gtgcttgatt
    21841 tgattcgttc gggaatgagc gcggatgaag tggattctgt tctgaataac caatccggat
    21901 tgatcggatt atccggatat tcttcaaatc tgattgaaat aatcgacgaa agcgaacgag
    21961 gaaatgaaga ttgtcagctg gcatatgacg cttacagcca tcgcttaaaa agttatatcg
    22021 gtgcatttgt ctggctgttg gacggagcgg atgcgattgt atttacggac gatctcggga
    22081 taaaatcatg gaaattgcga gaaaaggttt ttaaggattc gcaaaatatg ggaatcagat
    22141 tagatgatgg tgcaaatcaa aatgcctctt ttgaaaaacc ttcgctcgtc agccgaaatg
    22201 actctaatat tccgatttgg gtagttccga atgatgagga aatagttatc ttgaatgaaa
    22261 tattagaatt aatagggaat ggcactttgt gatttatctg aattcgaata aatatatctc
    22321 gttttagaag aaaaatttac ggactttttg acacattttt tctgatacct ttaaaattgt
    22381 cacgatcgaa aaattgcttt tttatcaaag ataaaatcat gataaactaa acccaataaa
    22441 aaatctttaa gcgcgataca gagagatcgg caagattgaa tgaataggca aaataggaat
    22501 tcacctgaag gtctcgctgc gcgcgagacc tttttttatg ttaatcaaga aaaaagttat
    22561 ggataattca actctcacaa ataaaaggaa ccatatcatg aaagacgtaa tcattgcctg
    22621 cgattttaag gatgcgaaaa ccacactgtc atttttggat ttgttcaaaa ccaagaaacc
    22681 ttttgtaaaa atcggaatgg aactattcta tttcgaagga ccggctatcg ttcgcgagat
    22741 taaagctcgc ggccataaaa tatttcttga tctaaaactc catgatattc cgaataccgt
    22801 tatgtccgcc atgcaaagtc tttcaaaact ggacccggac attatcgatg ttcacgcagc
    22861 cggaacaacc gaaatgatga aagccgcaat ggaaggagct tttcgatccg acggatcaaa
    22921 accgcttgta atcgcggtaa cgcaactcac ttctaccagc gaagaaagaa tgaatcggga
    22981 attgctgatt caagccacta tggaggaaac cgtactgcat tatgcgcaaa atgcaaaaga
    23041 agccgggttg gcgggcatcg tatgcagtcc tcttgaagcc gctatcgtaa aaaagggatt
    23101 gggggaggat ttcattaccg tcactccggg aattcgcttg ccggattcaa tcaaagatga
    23161 tcaggcaaga attaccactc ccgttcaagc aagagaaatc ggtaccgact atattgttgt
    23221 gggacgtccg atcatccgtg cagagaatcc ggtcgcggct tatgatcggt tttgtcacga
    23281 ttttttaggt aaagcttaga tcaacggagt atgattgaat caccattcag gaggatagaa
    23341 atgaaaaggg aaatcgctca gcatctgtta gatatcaaag ctgtttttct acgccccaat
    23401 gaaccgttta cttgggtcag cggaattaag agcccgatct attgtgataa tcggcttacg
    23461 ctctcttatc cggaaattcg taaagatatt gagaacggct tggtttcgat cattcgggaa
    23521 cattaccctg attgtgaagc aatatttggg acttctaccg caggaattcc tcacgcagcg
    23581 atcgttgcgg atattatgga ccttccaatg ggttatgtca gaggaaatac taaagaccac
    23641 ggacgaaata atcaaattga aggccgtttt gaaaagggtt ggaaagtggt cgtaatcgaa
    23701 gatttgattt caacagccgg cagcgccgtt gaagttgttc agattctccg tgaaaacggt
    23761 tgtaatgttc tgggaattgc ttcgattttc acttacggaa tgcaagcggg gttagataat
    23821 cttcgaaaag cggaagttca aaattattct ttgactgatt ttgatactct cattgaagtg
    23881 gaattggaaa acggatcgct ttcaagagcg gatgtcaata aattgttggc gtttcgggca
    23941 aatccttcag acagttcttg gcaattttgc acctccgctg tcgaaaatcc gcaaatttat
    24001 tgattcaagc cgatttcatt atttatcggt tcccgataaa tcggttgatc acgggtctga
    24061 ttcgtacata tgaatcagac ccgtttattg aaattcggca ttgccgtctg ataatgaata
    24121 ttcattgaaa agcaccttta ttgcaatcaa attcttataa tagaggcgat aaataaggat
    24181 caataattca agacaactct gaatcaattc aaacatcttt cctatttaat cacaaaaata
    24241 tctcttatac ccgttttctc atacttgaca aatcattgag cttgacttat atataagctc
    24301 gcttaaggat taagtatgat taaccaaata cattctttgt tatccgcttt taccacttgg
    24361 acaggtctgt cgatgaatta ttccatgcat ggattcgtgc gattcatccg cgaaaacgga
    24421 ctttccttat ctcaaatttc gacccttttt caactttatc attgcggtac ctcggcggtt
    24481 tctcaaatca gttccaattt agatatttca aatgccgctg tcagccaact gcttgagcgc
    24541 cttgttcagc agggtttaat cacacgatca gagaatcctc tcgaccgtcg tgaaaaacag
    24601 cttgtgttga caaataaagg atatgaattg ctaagggata gtgcaaaagc ctgcagtgca
    24661 tggcaagaaa aacttatttc taccttaacc cctgaagaac aaaataaagt atcggaagca
    24721 ctggaaatcc tcatagataa aacgaaacag cttccgaacg cataattatc cgattatgaa
    24781 aacctatgtc aaaaaatatc tcccgttctt cactaatctt gatttcctaa taacggagtt
    24841 ctaaatgcaa cgtttattaa aatatttaaa accatttatt tttctgctca ttcttgcgat
    24901 cgcattgctt tttttacaag cgaacgccga tctggcattg cctgattact tatcgaaaat
    24961 cgtcaatgtc ggcattcagg ccggaggaat tgagaacgca atcccgcagg caattcggca
    25021 aacggaaatg gaacgtttga tgcttttctt ctcagccgaa gaaaaggagc gggtattaaa
    25081 tgactacacc ctggtggacc aatcttcacc ggattacaat cgctacctat cccaatatcc
    25141 cgctctgaaa gacggtgcaa tttatgttct taatccgatc gatcaagccg aaacggattg
    25201 gttaaatccg gtcatggcga aagcgcttct ggtggtctac ggtatcgagc aggggatggc
    25261 tgatcctacc aaagttaccg ctctcggaca gatgaatttt aatggttttg atatttcaaa
    25321 acttccgccc ggaaccgatc tctttaaata catggggcaa atgccgccgg cagctctttc
    25381 gcaaattagt gaaagcatca ataagcgttt ggaagccatg ggaagcagca gcttgattca
    25441 ggcagctgcg gcaccgatcc aagccgaata tgcggccttg gggatggatg taggcgctgt
    25501 ttctcataaa tacattctaa ataccggaaa attaatgtta ttgttgacac ttctttcggt
    25561 gctctgtaac atcggcgttg gatttctttc gtcacgaatc gcctccggat tctcccgaaa
    25621 tttacgaaaa gctgttttct caaaagtgga aagtttttcc agtggggaat ttgaaaaatt
    25681 ctcgacagct tcattgatta cacgaaccac aaatgatatt acccagattc aaatggtagt
    25741 tattatgatg gtacggatgg ttttctatgc accgatcatc ggtgtcgggg gagtgatgcg
    25801 tgcgattaac aaggactctt ctatgtggtg gatccttgcg cttgccgtgg gtatattgat
    25861 tttattaatt attattattt tcaatattgc cgttccaaaa ttcaagatta ttcaaagttt
    25921 gattgaccgc ttaaatctga tcacgcggga gaatctctcc ggaatgatgg tgattcgggc
    25981 tttcaaccga caggactttg agttgaaacg ttttgataaa gccaatcggg atctgacctc
    26041 aaccagtctc tttgtcaacc gcgtcatggc ggtcatgatg ccgattatga tgctggtttt
    26101 gaacggaatt tcacttctga ttatttgggt cggtgggaaa caggtggcgg cttcaaatat
    26161 gcaggtaggc gatattttag cctttatgca gtacggaatg cagatcattt tcgcttttct
    26221 catgctttcg gtgatgttta tcattcttcc gcgggctcaa gtatctgcca accgtattgc
    26281 ggatgtcctt gaaacggaac cttctgtggt tgatccggag aatccgcaat ctttcccgcc
    26341 cgatgggaaa ggtttgttgg aattcagaaa tgtctcgttt cgttaccctg atgccgaaga
    26401 atatatctta cacgatatct ctttcgtcgc tcaaccgggt aaagtcaccg gtattatcgg
    26461 tccgaccggt tcgggtaaat cgacattggt gaacctgatc ccacgttttt atgacgttac
    26521 cgaaggcgct attctgatcg acggcgtgga tataaggaac gtgactcagc atgagctgcg
    26581 taaacggatc ggtttcgtcc cccagaaaag ttctttattt tcgggcacga ttgaatcaaa
    26641 tctgcgtttc ggaaatgaga atgcaccgga agagctgctc tataaggccg cagagattgc
    26701 gcaagcaact gaatttatcg aagcaaaacc ggaaaagatg gatgcggaaa tctcccaagg
    26761 aggcacaaac gtgtcaggtg gacagaaaca acgattatcg attgctcgcg ctttagtcaa
    26821 gcaggcaccg attaacatct ttgatgacag cttttcggct ttggatttca agacggatgc
    26881 cgctctgcga caggcactga aacagcaagc cgtacccggc actctattgc ttgtgactca
    26941 acgagtggcc agcattatga atgctgatca aatcattgta ttggatgaag gacgtatcgt
    27001 cggtaaagga aaacaccaag acctgatgaa aacttgtgaa atctacaaag acattgcttt
    27061 atcgcaatta agtatggagg aattagcatg aacacagaga ccacaaacag acaatccatg
    27121 ccacgccatg gcggtggtcg aagaggtcgc atgacaggtg aaaaagcccg tgattttcgc
    27181 ggcacgatga ttaaactcat taaatatctg agtgtttatc gaaattcaat tttgattgtt
    27241 gttctgtttg ccgctatttc caccgtattt accatcatcg gccccaaaat attaggccaa
    27301 gcaattacaa aattatttga aggctcaatc gcccaattaa ccggaacggg aagtattgat
    27361 ttcgatgcaa tcggtaaaat tcttttaacc gtcgcaggac tatatttaac ctctgccgcc
    27421 ttcagctacg gtcagggttg gataatggcg ggagtatccg caaaaattac ctaccggttc
    27481 cgaaaggata ttgccgaaaa gattaaccga atgcctctcg gttattttga aagcaccaat
    27541 cacggcgaaa ttctttcccg agttaccaat gatgtcgata cggtaagcca tacactgaac
    27601 caaagtctgt cgcaaattat cacttcatcg attacggtta tcggtgtgct gataatgatg
    27661 ctatccattt catggctgat gacattggtg gctttggcaa tcatccctct ctccttgttg
    27721 gtcgtcagcc tgattgttaa acaatctcaa aaatacttta agcagcaaca agattatttg
    27781 ggtcatgtca acggacacgt ggaagaaatg tatggggggc atatcgtaat caaagctttt
    27841 aatggggaag aaaagagcat tcaaagattt aatcaactga acgataaact ctatgaagcc
    27901 gcatggaaat cacaattttt ctccggaatg atgatgccaa tcattaattt tatcggcaat
    27961 atcggttatg tagccgtttg tatcctcgga ggatatctgg cggtccaaaa aaccattaca
    28021 gtcggtgata ttcaagcatt tattcaatat gtccgaaact ttacccagcc gatcacacag
    28081 ctcgcaaata tttcaaatgt cctgcaacag acagcggcag cagcggaacg tgtctttgag
    28141 ttcttaaatg aagcggaaga atcgcccgaa acagagaatc cggtagatcc ggaaacagtc
    28201 agagatcgag ttgaattcaa aaacgttcgg ttcggttaca acccgcaaaa aatcatcatc
    28261 aaaaactttt ccgcaaatgt ggattccggt caaaagatcg ccatcgtagg accgacggga
    28321 gcggggaaaa cgacaattgt taaattactg atgcgcttct atgatgttac cgatggggcg
    28381 attttaattg acgaacataa tgtgaaggat ttctaccgta aagatcttcg gcaaatgttt
    28441 ggaatggtct tacaagacac ttggatgttc aacggaacca tcatcgataa tatccggtac
    28501 ggtcggccgg atgtaagcga tgaggaagtt attgccgcag cgaaagcagc tcatatcgat
    28561 cacttcattc gtacgctgcc ggagggttat aattttgtat taaatgagga aacaacgaat
    28621 atttcgcaag gacaaatgca attattaacc attgcacgcg caattttggc aaatccaaaa
    28681 atgctgatct tggatgaagc aaccagctcg gtcgatactc gtaccgaatt gttgattcaa
    28741 aaagcaatgg atcatttaat ggcaaaccgc accagtttta ttattgccca tcgcctttca
    28801 acaattcgtg acgctgattt gattttagtt atgagagacg gagatattgt tgagcaggga
    28861 aagcacaaag aacttttggc tcgcggaggc ttttatgcag agctttataa cagccagttt
    28921 gaaatgggtc agacaatttg atacgttctg aaaaatagaa tataggcata ttgctataat
    28981 ataaatatgc ctatgttctt cttttataaa aagggaggaa aaccatggaa gaaaagcttt
    29041 tttctgtcac tataagtcat cagataggca gcggaggcgc ttacatcgga gagaaactaa
    29101 gcgaacggct aggtattccc tttctggatc gtgcaatttt gaaagaagta tcaaaccaac
    29161 tgaatctggc tgaatctgaa ttggaacatc gtgaagaacg gttaagttct ttttgggaaa
    29221 acttcaatcg cttggtcgca tacacaaatc caacaacctc gttaacctcg caaacttatt
    29281 tccctaccga caaagactta ttccaagtgg aatgtgatac gatcaagcgc attgccgaaa
    29341 aatcctcagc tatcttttta ggacgttgcg gctattacgt tcttcgggat catcccagaa
    29401 aagtcagcat catgttgatc gctgaccaat cgtcaagggt aaaacgttta caagaattgt
    29461 ataaaatatc tgaaaaagaa gctttagaaa tgatccgaac caatgatcgg gagcgatcgg
    29521 aatatatacg cacttttacg aaacaggatt ggttggatgt tcgtctttat gatatctgta
    29581 tcaataccac atccgtagga ttggattgtg cagcggatct ggccgaaaat tgtatcagag
    29641 cgaaacttcg attaacggaa aaataaatct aaccggcaat tttcttgccg gttagattta
    29701 aatgaaacac aattactcta tagtcagcat aataacacct aaaccggcaa aaacacatcc
    29761 gatcagcttt tcgagtgttt gttcattgac cttatttgcc aagcgagcgg ttaataaccc
    29821 cccgacaacc gatccgattg caatgataag ccccgaaccg aaatttacat ttccatggat
    29881 agcatatccg attgtccccg atgctgcggt cagcgacatg atcaaagaag aagtgccgat
    29941 tgccacatga gtggaatatt ttagaacggt tatcataacg atcaaaaatg cgatcccgcc
    30001 gcccgcgcca aacattccgc tgaatatacc caacgctaat ccgattccaa atgagatagc
    30061 cgcatttttt acggtaaatg gtttaatttg gctgttttcg acggctggcc tttccggacg
    30121 agttgatttt acccacattc cgattgccat caaaaaaatg ataataataa atccgatgct
    30181 cataccttgt cctgaaatcg cttcatttgc aaaggaagag ccgatctgag cgccggtaac
    30241 ggaacccaaa gctaaccata aacccgcctt aagattaata ttgccgcttt tataatagga
    30301 ccatgccacg aataaaggtg tgattgtatc aataaacaaa ctggttccga ttgccatatg
    30361 cacaggaaaa cccatcaaaa ccgtcagaag gggcacaacg actacaacac cgctggctcc
    30421 tgttaatccc accacgcatc cggttattaa tccgatcata attaaataaa caatttgcat
    30481 caatatttct cctctgatat gatttttaaa aatatatgct ctattccata ctataaaaaa
    30541 ccaaccctaa agtaccggga cccgcatgaa ctccgataac cggcccgatc tccgctttaa
    30601 tcaattgaac gtaattaaat ttctcgcgaa tttgattttc caaaacataa gcttcctcct
    30661 cggcatctcc atgaagaatg gctacatgaa ccggcttatc tccgattctt tccttcatga
    30721 tttcaaccaa gcgcataatt gattttttac gggtatattg tttttccaac ggttcgatta
    30781 caccatcttt aatataaaga atcggtttga tcgccaaagc agaaccgaga aatgccgtag
    30841 ctcccccgat ccgtccccct ttataaagat attccaaagt tcggaccgta aaaataacat
    30901 tcattctctt cttgatttct tcgactaaat gaataacaga ttccgcatct tgcccttctt
    30961 tggcagcttg agcggcggca attgccatca tcccctgccc caaagaaacc gattccgaat
    31021 caataatgtg taccggtact ttcgtaaact gcttcttagc catttctgcg gattgaattg
    31081 ttgcactcat tttgctcgaa attgtgatcg tcacaatttc ctccgcttca tccgcaagct
    31141 ttgtaaagat tttaagaaaa tcttcagcgg tagtttgtga ggtagtcggt aattttttac
    31201 tggcacgttg aagttgataa aactccgggc ccccaaggtt gactccggca atgtaagttt
    31261 tatcgtcaat attaacatag ataggtgcca tatgaattcc gtattcgttc gcttgttcca
    31321 acgttaaaga acacgcaccg tctgttacaa tagcaacttt ttgcttcata gttttttcct
    31381 ttccaaagat caaaaatctt ctgtggtgaa ttcctcccga ttgtgaaata gaaaccaaaa
    31441 ttttccgaga aggactttca ttgaggtagt atatttacaa atttcagcgg gatccgcttc
    31501 cgaacccggt aaatcataat gaataggtaa aaggtaattc ggttgaaaac tgtttgccaa
    31561 tttaatcaag ttttctttat attttttgtc tcctacagga gaggtgcgcc aaggcaagca
    31621 gaaaatatct ggatgaacgt ttaaaggtgc ttcatttgca tcacccacgt gcaaaacact
    31681 gatttcatcc cgaactaaaa aagataaagg agttcctccc ggttccggaa gtcttctgcg
    31741 agtcagtaaa ttcataaaaa cataagcagg ttgattggtc agactgtaac cgggaaggat
    31801 tgtgaccgaa tcgatttcct gcccgggtaa aaccggccgg attcgtgaag aaggaattcc
    31861 tctccgctct acgatttcac aaatgtccgc cgacgccaaa attgttccac tttgaacttc
    31921 cgcaaccttt ccgatatgat ctttatgcgc atgagttaaa caaatatact caacacccgg
    31981 ttggggcgga aaagtaaaat cgggatcgat caaaatatga tgtctcccga tgatttcgac
    32041 acaagaatga cctaaataac gtattttcat cattgcctat tcctgattca ttgccgattt
    32101 gtcattttca ttcccttttt cggtaatacc ctcgaaattt attctttatt ccctttgagg
    32161 aaaatacact gagatgatct caaaaatact tatatattct tctccaaatg agatggtttc
    32221 ttctttacga tccgccaaac ggtaattgca ctcagaatat atccgatcat aaatacaaac
    32281 caattaaatg aagaaggttt taatgaactg agctcttcgg aagtagccgg aagaatggaa
    32341 atggcatcct gcataaatac ataaagcatg atcaagattc ccaaaccgag aaaaatgccg
    32401 tctgagattc ggagacgaac gattatcccc tggtcttccc ttcggacaat taaaattcct
    32461 ccaataatca tcagcaacga aatcaacacc ggagcgatta ccggccccca ccacggcaac
    32521 ggaatcagaa acagaagatc ggtatcaaaa atcgatcgcg gccatgatat aaccagtgcc
    32581 agccaaatat agtaaaagat atcccaaatg ccgaatgcga tcaacgcaaa actcagcttt
    32641 gattgaattc gttcaccggc aacccaaccg attgccaaca gcatgacaag cgttgccagt
    32701 tctctgccca attcaactgc agcgatctcc ggatcaaagg gaggaacact gagcattaaa
    32761 tcggttatcc cataaatacg tcttaaatag acaacgacgg cggtctctaa aaaagccatt
    32821 gcaattgcat agattccgac ccaagttatt tttccggata atttcatgag cttcctacct
    32881 ttatgatgaa attccaaata attgaggcgc taatgcaaat ttcccttttc cttcaactgc
    32941 tattgatcca tccgatcgat aaataatgcc ctccgcattt ctcgaatgag ttccttcgga
    33001 ggttacccgg cattcaacac gaacttcttc cccgagcgga ataggtttac gataattcaa
    33061 ttcaagatga accgttgctc cgtgaaaacc gctctcccag accgcgatgc ccaacgcttc
    33121 atccatcaaa gccgctgagg cacccccgtg aacataacct ttgggacctt cttggaaaag
    33181 ttgaaaagta tagcgaattt cgatccgtcc attcggaagt gtttgccaga caacatcatc
    33241 ggctttcaaa ccgcaagacc caaagacata ttgtgatcct gttttcggta aactcttcac
    33301 ttatggctcc ttgtgttgag gaacagattt ctgattcgta ggttctctgc gaatccactc
    33361 tttttgatcg ggaatatcgc gatcgtaaaa aatacgaaga aaaatcactt ttttccgctc
    33421 ttccggatct ttcggcagcg ggcgccggtt ggcttcaaga cgtttaacga cggtaatcag
    33481 caacattccc aacgacaacc ccaagatagc attcggccca ttaaaaatgt ttgcaaaaac
    33541 aggcatcaaa agaatcgtaa ttaatggaag cggcgcaaaa gtcaacagac aaccgattcc
    33601 cagtgaaaga agcatgatga aacaacctaa aggaaaaaga gctaatagat ttcctaaaaa
    33661 aggactcagc ccacgaccac cgtgaaaatt aagatagata ggccaattat gccctataac
    33721 tgctgctaat ccgaccataa ctgcggcaga ttcacccaaa ccgaatctca aagtcatcca
    33781 agttggcaga aagcctttga aaatatcaaa aagccctacc ggtaccaccg cccatttagc
    33841 gacatgttcc caaaccatcg aaccgctgac tgttccgcta ccgtattccc gtaaatcaat
    33901 tccacgtatc catttaccgg caagatatgc agacggaatc gatcccagta aataactacc
    33961 gataatcaga aggattaccg agataaacca ctgcacatct attccctgat ttggcataat
    34021 atgacctttc cattaattta atattttttc tcaatcccca tcattcccaa taatacgcca
    34081 aaccgatcag ccccggtccg gtgtgagctc ccataaccgg tgtaaattcc actatcggga
    34141 attcattctt cgggaaagct tccattgcgg ctttctgcag agcctccgcc ctttcatgcg
    34201 catcggcttc catcacagca aagcgagcct tatgatatgc accgatttgc ttttttatca
    34261 aagaaaccat tgtgggaata acctttcgtt tcccacgaag attagctacc ggggcgacaa
    34321 tcccttcttg atcgattccg agaatcggca gaatctgaat agctgtcccc aaaacatatt
    34381 gggctttccc gatccttccg ccgcgagcaa gatattccaa cgtatccaag gcagccgcta
    34441 tcccggtcct ttttctggct ccgtccaaaa aagttaccac atttttgatc gacattcctt
    34501 cttcgaaacg ttgcgctgct tcgattgcca aaaaaccttg tgcaatcgtt gccattagac
    34561 tgtcataaat tacaatttca cagtcatgat atttctcacg aatcattttg gcggcgtcta
    34621 acgctgcatt gtaagtgctg cttaatttac ttgaaagtga aacaaataag atctccgaga
    34681 cgccttcttt tatatgcttt tcaaagcttt gataaaactg cataatcgtc ggcgcgcttg
    34741 tcttcggaac aatcttatca ttccgcatct tctgatataa ctcgtgtgca gaaatatcaa
    34801 tcccatccaa ataattctta ttatcgaagg aaattaataa cggaatcacc tcgataccgt
    34861 atttgtctat agcttcttta ggaacttgag ccgcactatc tgtaacgacc accgattttc
    34921 tcatcatcat ttctcctcaa aggtaacatc cgcaggcatt cccggtttca gtttcaaatc
    34981 gggattcggt acatcaattt ttacggcata tacggtcgtt cttcgtcctt ccatcgtctg
    35041 cacattccgc ggagtaaatt ctgcctgatc ggaaatatat acaacggcgc cggtgaaagt
    35101 ctcgtccggg aacgaatcaa cctgaatgga taccttttca ttcagtttta cttttccgta
    35161 ttccgtttcc ggaatatata ctgtcaaatt cacattctct aattggccaa ccgttaatac
    35221 aaccgcattc ggagcgattg tttcacccac ttcaatattt cgagcaagaa ttattccatc
    35281 gacgggagcc tttacgatag tcttctccaa ttgaacatca attaccgaga gcgcagcttg
    35341 tgcttgtttt aatcccgctt ccgcctggct gacattggac tcagccactt ttacttgtaa
    35401 agactggtca ccgcttaata atgaattgta ataatcgaga gcccgatcat aacgttcctg
    35461 agtcactcgg actctggctc ttccttccag tacatctttt gcggcttggg tagttaaaag
    35521 acgatcataa ttggactgcg cggaatccaa ctctgtttta gcggtgtcat attgatcttg
    35581 agcgaaatcc tgtaattctt ctttatcttt cgcattggtt gcttgcgtga gaacttgatc
    35641 ggcaatcagg aaggaagctt ccgcattcgc caatcttttt tctgcattta aaaaccccgc
    35701 ggaagcttta tcgttaagta ttttttccaa actggctttt tcttcgtcaa ggtccgcttt
    35761 tgcggcatcc atttccactt tggcggaagc aagtttttcg gttttatcaa aataccatac
    35821 aggtaacttg aattggctgg attgttcggt attccaactg tttattcgtg tttgtcgttc
    35881 ctgttgtcga gccgaatcga cagccagatc atattgagtt tttgctgtgg ctaatgccga
    35941 ttccgccagc gtaacggaag catcggcttg atcacgttgt gcctttagca aagaatcgtc
    36001 tatgcgaaac aattcatctc ctttttttac ttgcgcccct tcttccgtgc tcactgatac
    36061 aaccgttccg cctatttgag gagcaacatc gacttgtacg gcggaaattg ttccggaagc
    36121 tttgagtgaa gtgctttgac cgttcaggtt ttcacacccc gaaagaaaca aacacaagat
    36181 gataaagagc gagataccga ttgatgattt ctttttcatt ttaaactcct gttaaagaag
    36241 taagtttttt ttgttgatta aacaattaca gacgtttgtg aaacatcaga acgctggcaa
    36301 agaaaacaac gatactgaat acagccatcg gccagatcca cttccatagg atgacaaaat
    36361 cggctccttt gagaataatg cctctgacaa tttcaaggaa ataagtgacc ggcaacagat
    36421 atccagcata ataggcgagt cccggcatgt tctcacgcgg gaacaacagt ccggcgagaa
    36481 taaaagcagg gatcataata aatgatgcca tatacatggc ttgcatctgt gtctttgaaa
    36541 tattggaaat gaaaacgccc agtcccaggg aacccaacaa aaaaatcaga ctcaggccgg
    36601 ctaataaagc ataactccct acaactttca ccccgaacca gaccgcgcca accgctacag
    36661 tcatcaccac attgataaat gcaacaacaa caaaaggcag aatcttccca agcataagtt
    36721 cccatgattt gatcggagta acgatgagtt gttccaatgt tccctgctcc ctttcgcgga
    36781 caatcgcaaa tgcggtcagc aataatgctt gaatttgcaa gatgattgcc accaaaccgg
    36841 gaatcatgaa attcatccgt ttcatatcgg gattgtataa ataacgaagt ctggcatcaa
    36901 ccggcaggct gatttgcata cccgttccgc tgcgttccag acgctgaatc agaatttcct
    36961 gtgataagga ttggctaatg gtttccgctg ccagttgtgc ggtttgagcg attgtgggat
    37021 tcgtcccatc aatataaaat tggatggtag ttgatccgcc ggtatatatt tctcttccgt
    37081 aattttccgg aataaaaagt ccggccttaa ctgttccttc atccaacatt ttcaaaatct
    37141 catcttcgct ttgagcggcg tgagtatata ggaaaaattc actgcccgtc agtttgtcga
    37201 tcaaagttcg actggcatcg gtatgggata aatctgccac ggctaacgga atatctttca
    37261 catcatttgc gacagcatat cccaacaaga ataacatcat gacgggcata atgatcacca
    37321 agctaagcgt acgtggatct ctgataatat gaatccattc ttttcgaaca atggtcatta
    37381 tgcggtgaaa actttgcctc ataggtatct ccttattcga ttccggctcg aacatgcatc
    37441 cgattttcct gattaactaa cgatacaaac acatcttcca atgagggtaa aatccgttcg
    37501 atccgagcaa ttttgatccc gttttcttgc aatgtatttt ccaacatcat cttatcgctt
    37561 tcatccttta ccaaaacatg cagcaataca ccatgagccg ccacatcttg aatattgtct
    37621 ctttttttca agagctgttc ggcgtgagcc ggttcatcgc aatcgatttc aaataaaacg
    37681 ccgttcattg cttccttcag agaatccgga tcattcaatg caatcaatct tgatcgatac
    37741 atcataccga tcacgttaca aaattccgcc tcatccatgt aatgggtagt tgccaaaacg
    37801 ctgacccctc gtcccgcgag ggtataaatt aactcccaaa aatcatgccg ggaaaccgga
    37861 tcgactccgg cagtcggctc atccaaaaaa agcatcggcg ggtcatgaac aatggcgcac
    37921 gccaacgcca accgctgccg ccaagcaccg gaaagatctc tggttaattg tttctcatgc
    37981 cctcttaatc ccgccatttc aatcaattcg actttccgag tcttcatcgt ttcttgcgga
    38041 ataccgtaca gatcggcata aaattttaaa ttttcagcca ctgtcaaatc gttataaagc
    38101 gagaactttt gagacatata cccgattcgc ttttttactt cttctggttt ttttgccaca
    38161 tcataaccga gaattctggc ttctccggat gtcggtttta aaatgccgca taacatacga
    38221 atcgttgttg tttttccggc tccgttcggc ccgagaagac cgaaaatttc acctttcgga
    38281 atatcaaaac tgacatgatc cactgcgaca aaattaccga atgttcgtgt aagttcagaa
    38341 acctgaacag atttttcgcc actcatgcct gtttttcctt ctgttccatc aaatgaataa
    38401 aaacatcctc cattccgatt tttgcttccc gaagaatgct gacatctgcg ccggcttcta
    38461 ttaactcgga tctcaagtga gattgagcgc caacaggatc attaaccgaa agtcgaaggc
    38521 gatctccgac cgcccgccaa ctgagaactt ctttcgaatt gtccacgact ttccgcagaa
    38581 ctccgcgcgg tttggctttc acttcgatca tttgaaaagg caattgcttt tcaagatcaa
    38641 gtggtgaacc ggaaaccaac atctttccct gaaaaatgat gctcaccacg tcgcagcgct
    38701 ccgcttcatc catataaggt gtactcacca aaacggtcac gccgcgctcg atcacttttg
    38761 ccaataactg ccataattcc cgccgtgaaa cgggatcgac accggtcgtc ggttcatcca
    38821 acagaagaat ttcgggttca tggattaaag cacaagctaa agccaatttt tttctcattc
    38881 cgccggagag attctcactg cgtcgagaag taaaatcgga taagcgggcg aaatctaaca
    38941 aatcatcaat tcgctctttc tgttttgatt tcggaacccc gtttatgtct gcaaaaaatc
    39001 ttaaattttc tataacactc aaatcgggat agagactgaa agcctgaggc atatatccca
    39061 agtgaggtcg aaccttttga gcttgtttca gaatatcgta acccgccact ttagccgttc
    39121 cggatgtcgg cagaataacg gttgataaaa tcctgatcag agtcgttttc ccggctccgt
    39181 cagggccgac cagaccgtac agttggcctg attcgatttt cagatccacc ccgtcaagtg
    39241 ccgccggatc catttttccc tggttggggt aacgcttgat aagatgatac gcttcaatcg
    39301 ttgtagtcat gagacaaaaa cctttatcaa attaattcac tttctattat aaaagtaaac
    39361 atttcactga tatcattccc caattgttta cataattggg gatccatttc cataaccaaa
    39421 gaacgatatc gttccatata agggacaatc gatcgatcgt tgtccaggtc gataattttt
    39481 tcccgtgtta ccattccaaa aataaatccg ccaaagaaaa gcaaccccga tcgggtagta
    39541 ataattcctt ttttaccgac aaattgcgag atccatttga gtaagaaacg acaacttgag
    39601 cagcttttta gccctgtgat taaatcggtc aaaacattca agcaaaaacg taaattatcc
    39661 ggcgtatcaa tcgaaatttg atttaaaacg atttcagaca aagcatgatc ctgaatgatc
    39721 tgatttggtt gaactttttc cgcaatttcc gtttcatcag gattcgggtt ttccgtcttt
    39781 gtatctggta tctcttccgg gaaacggcta taagccaatg aagtcggcgt aaatacaatc
    39841 tccgaacgtc ctacggatgg tttattggaa ttaatttgat actttgaagt gacatatcct
    39901 tttttctcaa gaagacgtaa catatcatat gcggtaaacg aactgacccc gattttcttc
    39961 gccaataaag aataatggat gggtttatgc atttcccgat ataaatcaac aaacaaatgg
    40021 atgaattttt cctgccgtct tgtcagttcc atgtttttcc caaaaagacc aaaatattaa
    40081 tttaatttta ttgacgatag aaatcattgt caagtagagg tgttcaaatc gttaccattg
    40141 ttattcatcg gaattttccg ttataataac gagcacaaca atagtattcg gaaataagtc
    40201 attgagaaac ttcctcataa aaaggaagtt tgttgatcaa taaataattt tatatctgat
    40261 tcaataaagg tgccgatctt acgcataaac gttcagaatt tctttccata tgtgcgggat
    40321 ttgcggtcaa atcattcaat tcaatatttt tatgtatcgt tattaatgaa tgaggataat
    40381 tggtgaaaac gcaaaaccat cgtaaagaag aattatgcgc tcgagaagtc gccggaaaaa
    40441 ggaggaagca tgaaaaaaat gcgttggttc attgtgattt tgatcgcgct gtttacagcc
    40501 ggatcggttt cggctcaaga ccttcaagcg acaaatcaag cattggagct ggagatcaat
    40561 atcctgaagc ttcaggcgac aaaacaagct ttggaagccg aagtaggaca gcaaaacgaa
    40621 cctcagccaa gcgagccgat tgaagatgcg agcggcggga tgatgaactg gggaagtcaa
    40681 aactcccctt atgttcccac accgacgcct ttcaacaacg tttcggttcc gatcagtcag
    40741 caaaactttc cggcgttggt acgaccgcct atcccgcaag gtcctcctcc gacctgggac
    40801 aaaccgagaa caaaccgaat aacaagaatt tatgtgggaa actcgggtca gtttaaaacc
    40861 atggcagatg ccttaaagaa tattcccgac aaagccggtg aggtcatcat ttatttgaca
    40921 agtgacacag aagagccgga tgacggcttc ggaatcccga ttgataaaga aattacggta
    40981 gttcgtatcg catcggatac gggtgctaaa agaaccgcat ggccggcaga ccggtcattt
    41041 tggtttttct gtaatggtgt gccgttaatt atcgaaccgg aagtggaatt tgcggggaaa
    41101 agcatgatta tgggcggcgc tcttacctac gccagacata atgtccagac tcctaaaagc
    41161 gtcattatca tcaacggctc cgcatggtgg gtttatgccg gaggacagtc cgaccgagaa
    41221 ggacatagtt cgacggtcga taatgcactc gtcatcatca acggaaatgt tgnnnnnnnn
    41281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaggaca tagttcgacg gtcgataatg
    41401 cactcgtcat catcaacgga aatgttgacc gtgttcatgc cggcggtcgg gcaatttatg
    41461 gggaaaccat tgtcaatcgc gcaacggttg tcgtcaacgg gaaagcaaat gaagtttact
    41521 gcagcggcta caccgaaaat cccagcgcaa aaacgacggt cggcaatgca aatatgctga
    41581 tctatggttg gtatgggatt tacggattgg ctcgcgggca aggtcaggca tatttgctga
    41641 atcccgccgg atgttattaa tcttctgctc gggggggaaa atcgaatcat tcagaataaa
    41701 caaaatatcc tgttttttta tacaggatat tttgtttgtt acactgttga tcgaaagtgg
    41761 gcggaacagg actcgaacct gcgaccccat ccttgtaagg gatgtgctct aaccaactga
    41821 gctatccgcc cgaatgacgc agcgaattat aacataaacc gctggaaata ttcggtttcc
    41881 ccaatgagaa atttcactct tttcgattga tgctcgcctc agcactgaat ccgttcggat
    41941 atcgcttggc gactttctcg atatttcttt ccgcaacttc ttccaaatcg acaccgatat
    42001 catgggcata gacggccaca taccagagaa tatcccccaa ttcttcaatc actctgtctt
    42061 ggtcgtaagg atgtccgtga ataaatactt ttttggtccg gttggctaat tctcccgcct
    42121 ctcccgccaa tgccagagac caggccaaat gagtcatctt ttcgtccatg ccgccgattg
    42181 ttgtccgcat acattgtgct tgatattcat tcaatgtttc gatttttctt tccattttgc
    42241 ctctttccgt taataacttt catatatcca ccccctataa tagtatcagc gtcttgaaat
    42301 agattcttaa tattactaaa aataaccctt ttaaaaggta tataacggag aagaaaatat
    42361 gaattttgat aaatatacac aaaaagctca ggaggcagtt gcccagtctc aacagattgc
    42421 gattgaattt tcgcatcaag tgattgaacc ggctcatctg ttactggcgc ttttacggca
    42481 acaggacggt acggttcccg ctattgtcac acgggtttcc ggcagcgtcg ccggacttct
    42541 caatgacgtt gaaaaatcac tggcaaatca accgaaagtt tatggaagca gcggtaatct
    42601 ttcaatgtcg cggacgacgg cggatgtctt ctcaaatgcc gaaaaaattg ccaaggggat
    42661 gcaagatgaa tatgtatcca cggaacattt attcctggcc ctttgtgaca gcgtggaagg
    42721 aagaagattg gaacaattcg gactgacgaa agacagtatt ctgaaagcat taatgtcgat
    42781 ccgcggaagt cagagagtga ccagtcaaaa cccggaagat acgtatcagt cactggaaaa
    42841 atacggtcgt gatttgaccg ctatcgcccg tcaagggaaa cttgatccgg tgatcggtcg
    42901 ggatgatgag atcagacgga caattcaaat cctttcccgc aggacgaaaa ataaccccgc
    42961 tttaatcggg gaacccggtg tcggaaaaac agcggtggtg gaaggattgg ctcagcgaat
    43021 tgtcaaagga gatgtgccgg aaggtttgaa aaacaaaaga cttgttcaac tcgacatgtc
    43081 cgctttggtt gccggtgcaa aatatcgggg cgaattcgaa gagcgcctaa aggcggtttt
    43141 gaaagaaatt accgattccg aaggtcagat cattcttttc atcgatgaat tgcacacgat
    43201 tgtcggcgcc ggagctgcgg aaggctcaat ggatgccggt aatatgatta aacccatgct
    43261 tgcacgcggt gaattacatc tgatcggtgc aacaacctta aatgaatatc gaaaatatat
    43321 cgagaaagat gcggcgctcg aacgtcgatt ccagaccgta tttgtcgatg aaccttcggt
    43381 tgaagatact atcagtattc tacgaggctt gaaacagcgc tacgaagtcc atcacggcgt
    43441 tcgaattacc gatgctgccg tcattgcagc ggcgacatta tccaaccgat acataaccga
    43501 ccgtcaattg ccggataaag ccattgactt aatcgatgaa gcagcggctc gattacgaac
    43561 ggaaatagat tccaaaccgc agattttgga cagcgttgac cgtgaaatca tgcagcttga
    43621 aatcgaaaag caagcgctcc aaaaggagca tgatcaagct tccaaagatc gtttggcaaa
    43681 actggaagaa gaattagcca acctgaagga aaaatcaaac cagttgacag cccggtggca
    43741 taccgaaaaa gaagcaattc aaaacctgaa aacagtcaaa gaaaagctgg aatcattgca
    43801 cattcagatt gaacaggcgg aacgagccaa caatttggaa ttagctgcca gattacgcta
    43861 tggcgaatta ccggctttgg aaaaacaact tgccgcatat gaagagaagt taaaggaaat
    43921 ccaaaaagag ggtgcccttc ttaaagaaga ggtggatgaa gaagaaattg cccaggtcgt
    43981 cgcgaattgg actggaattc cggtttctcg tttgatggaa agcgaaaaac agaaactggt
    44041 gcatatggaa gaaagattgc atcaacgggt agtcggtcaa gatgaagcgg taaaagcagt
    44101 cagcaatgcg gttcgccggt cccgtgccgg acttcaggac cccaaccggc cgatcggttc
    44161 tttcatcttt ttaggtccga caggcgtcgg aaagaccgaa cttgcgaaag cattagccga
    44221 gtttttgttt gatgatgaac gcgccatggt ccggattgac atgagcgaat atcaggaaaa
    44281 gcacaccgta tcgagattga tcggagctcc tccgggctat gtcggttacg aagaaggcgg
    44341 acaattgacg gaagccgttc gcagacgccc ctacagtgtc atcctcttcg atgagatcga
    44401 aaaggctcac agcgaagtct tcaacgtgct gttacaggta ttggatgatg ggcgtttgac
    44461 cgacggtcag ggaagaacgg ttgattttaa gaacacggta atcatcatga cttccaacgc
    44521 cggatccgaa ctatggatga acggaggaaa ggttgtcagt cgtgatgcgg ttaacagagt
    44581 attacagaat acttttaaac cggaattctt aaatcgactg gatgaaatcg ttgtcttcca
    44641 tagcctgaca aaggaagatt tgaaacaaat tgtcgacatt cagttgaagc atgttcgcga
    44701 cctcttgatc cagcgcggcc tcatgttgga tattacgccg aaagcgatcg agtttcttgt
    44761 cgaaacaggg acagatatcg aattcggtgc ccgtcctttg aaacgtgcga tacaacatga
    44821 attgcaggat cctttggcaa tggaaattct gaacggaaaa tatgttcagg gtgacacaat
    44881 tcaagtggat ttagcacccg agggcgatca tctggttttt tcaaaaaagc agacctcata
    44941 ataagatcaa caaaaaaacc ggccaaagaa tgaaattggt cggttttttt aattattttc
    45001 ctttatgggt tacttttata aggtcaagaa taaatgatcg ctttgtttcc acccctgtta
    45061 taattgtttc cgatctaaac ctaacggagg ctttttatgt ccaacgtaac gattacaaac
    45121 cacccattga tcgaacataa actctcgata ttgcgtgatg ttcacaccaa cccgacggtt
    45181 tttcgccatt tggtaaatga attgggcgct ttgttgtgct atgaggcaac tcgtgatttg
    45241 ccgctttgcc cggtagaggt ccaaaccccc ttatccatga ccacagctta tgaaatgaaa
    45301 gaaagcatcg cattaatccc catccttcgc gccggactcg gaatggtcga gggcatttgg
    45361 gacttaatcc caaacgccca cgtttttcac atcgggtttt atcgcaatga aaagacatta
    45421 caacccgttg aatactacaa caagctgccc aaggatcatc cgttcaatat atctttcatc
    45481 ctcgatccga tgcttgccac cggcggttca gccatcgcga ccacgaatat cgtgaaaaaa
    45541 tggggtgcac aaaccattaa gtttgtcgga attatcggag cgccggaagg aatcaaagca
    45601 cttcaggaag ctcatcccga tgtggatatt cacttggcgg caatcgacga tcatttgaat
    45661 gataagggat atattgtccc ggggttggga gacgccggcg atcgccaatt cggtacggtc
    45721 ttttaataaa gattaaccga caaccttcgc gtagagtttg gcatcacaat aaaccccatg
    45781 aacagggagg cgctttcgca ttacaccttc ctgctccatc cccaattttt gcattaccct
    45841 ttcggattta aaattatcga tttcacattc ggcttcgatt cggtgaatat ccaacgttcg
    45901 aaccgccgca tcaatcatgg cgttcgcagc ttctacgata attcctctcc cccaatattg
    45961 gttggaaagg caataaccga gttcaaccac ccttaaagct aagtcaatcg aaacaaaggc
    46021 aatcgtccct accattttgt tattttcacg ccagacaatc cctcggtcta tttgaagttt
    46081 ttcggattca acctgatcga tatactttaa aaattcttct gtatcagcta atgtacgatg
    46141 agctgaccaa gacatataac gtgctacttc aggattggag gcatattcaa aaatatcatt
    46201 ctcatccgcc cgcgtaatag gccgaagctg taaccgttgg gtcagaatgg ttttgggaaa
    46261 taatgccaat ttatcagaat acataaattc ctcttatttc attaaagtcg aaatgacgta
    46321 ttataaaaac agcgtattga agtatgtttc aatacgctgt agcgataact tgcgatgtca
    46381 caaggattta ttataaaaga aataaacatg ctaaaatagt ggaacgtcat ataatcctta
    46441 tgacttttgt tatatcattt cgtgacaact tgttgtattt taccaccgtt gccaaaatat
    46501 gacactcaaa aaaataaaac tgattggaga aaaatcgaaa aatgttggtc ggcgctcgaa
    46561 tgacaaatcc tgtaattaca attggaccgc gagctacgtt ggagacagcc aaagatctga
    46621 ttgacaaaaa atcgattcat tcccttcccg tcgtagatcg tcagggcagg ctgatgggat
    46681 taattaccaa agacagattg gaagatttta ttcaaaaagc caagcaagcc gatgaaccga
    46741 tgccattggt ggaagatgtc atggttcagc aagtattgac cgttacggaa aataccccca
    46801 tagaggaagc ggcacggatc atcagtgatt atgacgttaa atccttgccg gtcgttcgcg
    46861 gtaattttgt cgtcggaatt attacagcgt cctctctgat tcgtatcttt cttgaactta
    46921 ccggcgcccg cgatcaaggg gtgcgtttga ctataatggt tgaagacgtc cccggaaaga
    46981 ttttggcggt gattcaaaaa atcgtcgaat tgaacggaaa tatttctact ttttccacct
    47041 attattcgga tgagaaaaat taccgcatca tgactctgaa aatcagcggc gtagagaaat
    47101 acaaattaaa acaagaaatc gctcccattg tttataaaat aatcgatatt cgctaaaagc
    47161 gactgatttt tggtctcttt tatttattat ggactttata aagtctcatt ttgtcctttt
    47221 attaagtaca gtaaaaaaat gcaatccaag gtttctcgaa ggtataattt gaataagcga
    47281 tgaatcatcg cgcatcaaca gccgatacaa cataattttt tgaatcggga gttgcaaaat
    47341 tcggattaga aagcagaaac aaatgaatga ttgctctgga tattgaaacc accgggttag
    47401 accccaacct ggatgcggtt acggaaattg ccgcattacg ttttaaaggg gctcgggtgg
    47461 aagatacttt tcaaactctt gtaaatccga accggccgat tccttccaat attacccaat
    47521 tgacccatat taccaatgaa atggtccgta acgcaccgca aatcggtgaa gtgatcggaa
    47581 aagtcgcaca attcgttggg aatgatatta ttgtcgggca taatatcagt ttcgacttga
    47641 atttcctccg gctgaaagga attttacaaa aaaacctttc atatgataca tatgatttgg
    47701 catccatcct gcttcccgat gcaccccgtt ataatctcgg cgttttggcg cagagaatgg
    47761 gcattatcgt ccgtgatgag caccgagcgt tggcggattg ttataccacc tatcaggtat
    47821 tcaatcgttt gatcgagaga gccgaatctt taccggcaga actgttggcg gatatcgtaa
    47881 atctttccga aggaatacat tggaatgaag cggaattatt tcgccaaatc attcaaaaac
    47941 ggattcaaaa cggcgaaaaa atcaaaccga tttataaaat aacttttccg accttcctga
    48001 atgagggcgg gaacaacgtc gatcctctga agccttcgga aaatccgata cccctcgaag
    48061 aagatgaaat ggccgcaatt cttgaatacg gcggagcatt cagtaaatat ttcccgaatt
    48121 atgaacagcg atctcaacag attgagatgc tgcgaacggt tactactgct ttttccaaag
    48181 ggaaacacat gtttattgaa gccgggaccg gaaccggcaa atcatttgcc tatttaatcc
    48241 cggcatttca ttgggcaaaa caaaatggtg aaagggttat tgtttctacg aatacgataa
    48301 accttcagga acaattgatc cgaaaagata ttcccgaact gaacaatgtc cttcaggagg
    48361 acttccgtgc agctgtctta aaaggacgtt ccaactatct ttgcccccga agagttcaga
    48421 tgcttcattc gcatggggtg gaaaatgcgg atgaattacg cgtactttcc aaggtcttgg
    48481 tttggttata tgaaggcggg aaaggcgacc gcggcgagat taacctgaac ggcccgattg
    48541 atcgcgaaat ctgggaacgc ttgaacgccg attttgaagg atgccgtccc aatcaatgcc
    48601 cccattaccg tgacggacaa tgtccgttct atgcagccaa agccaaagct ttaaaatcgc
    48661 atgtcattat tgttaatcat gcactccttt tgacggatat ggcatacgga aacgatgttt
    48721 taccggaata caagtacctt attattgatg aagggcatca tcttgaatca gccgctaccg
    48781 gagcattgag cgtccgtata acccaaacgg atttatccca tcaactgcag gaactcggcg
    48841 gttacggacg gggccttttg ggaagaatgg ataataaact tgcggagaaa ttaaagccgg
    48901 acgaatatca attgtttgtg aacgcgacaa cctttatttc ggaaaatggc gctttggctg
    48961 aagaagctct caaatcatta tttgagactt ttcagttttt tatgaaagaa gcccgtgaag
    49021 ggaaccccgt tacggtttac ggacagcaat tgcggattac ttcggctata cgtacgcttg
    49081 cgggatgggc tgatattgaa attatttggg acaaaaccga aaaatatatc cgggaattaa
    49141 ttgattccgc ggaaaaaatt gatcgttccc tgatgaattt ggatgatatc gatgaagaac
    49201 ttcgagaatt gatcgactcg tttcacggtg gattgaaaaa attatcggaa tccaaaaaaa
    49261 tggtaaataa tctgattgtt aaaccggatc cggaaacgat ttattggatc gaaatatcgc
    49321 ccgtaaacaa ccggttatcg ttaaattccg cccctctgca tgtcggaaat ttaatcgaaa
    49381 aaatgctttg gcatgaaaaa gaaagcatca ttgtcacctc tgcgacgctg acggctgata
    49441 attcttttga ttatattcgc gaccggttgt ccgctgacga agcggaagaa ttagtcgtcg
    49501 gctcaccctt cgattatgag aactccgttt tgctttttat tcccaacgac attcccgatc
    49561 ccaatcaagg ttccgctcaa cacatggtag atacctcgtt gatcaactta tgtaaggctg
    49621 tcggcgggcg gacattggcg cttttcactt cctatgcgca attaaaacgt acttctaccg
    49681 atatcgcccc tgaaatgcaa aaagcgggaa taactattta tgaacaaggg gaaggagcat
    49741 ctgcctcttc cctactggaa actttccggg agaccgatca agcgatcctt ttgggaaccc
    49801 gcagtttctg ggaaggtgtg gatatccaag gtgaaaagct ctcggttgtt gttattgtaa
    49861 aattgccgtt tgatgttccg tccgatccta ttttctcagc ccgctcggaa ggctttgaaa
    49921 accccttcag tcaatatagc ttaccggaag caatcttaaa atttcgccaa ggattcggcc
    49981 gtttgattcg ttctcaatcc gatcggggtg tagtagttat cttggataat cgcgttctga
    50041 caaaacgtta tgggagagaa ttcctcaact caattccaaa atgcacggaa cgaattgccg
    50101 gtattcgtga gttgccaagg gaagcacaac gctggct
//
LOCUS       DAOSMM010000009        49511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_757_length_49511_cov_18.555079, whole genome shotgun sequence.
ACCESSION   DAOSMM010000009 DAOSMM010000000
VERSION     DAOSMM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 49511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49511
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_757_length_49511_cov_18.555079"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..7529
                     /locus_tag="PK898_02695"
     CDS             132..7529
                     /locus_tag="PK898_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18974.1"
                     /translation="MNLKIINQDGWEKNVSIAKTVILLGPQAGNDIFLPIKEQFQIIH
                     SGPEKSDIKIFRFNSTSSSEDFFIFRNGESISVLPLEETELFPGDRILIGNYQIEFEG
                     TTIDKPFEPQNLEQQSKRRTNYWIWIALLFILLFAVGYFLQPKIIKFVSDQPTNTLVR
                     GDSAELSWEVSPFVTHLTITSDEETLPVSKTERFRVSPEKTTTYTLTAENRITRILHR
                     PHRAELVLAVNQPESESTLKSEKISKNKYKLEWEVTSTTRMATLVIGENRQELNSDQY
                     HGSQTVKIQEDTPVQLISESEMGKEVKNLVLKAENPQMELVKFIIWIRPSAPKTNTSP
                     VQRIDNSDGSSDEFTAKYAELVPDPSSLTGYKIVRYEFDRQIISGEQIMLEWEVKDAE
                     TVLLEPLSDQFVSQKGAQFYFPTQSTNFTLSVSDGSRLKSYTLPISIRTEKNVEAVAP
                     KIDLFDANPVTLTQPGKTTLSWSVSGDWTRIQLIASGASFDSIHADTNNENAIPLTIS
                     QNSGSILADYLAPTGFLSLNLKENASFILKAWSGNLSNSSIVNVRVNPSEPPAVLKSP
                     DLKINAIFTDSDNYTVGDTITVSTAFDGLSSIDSIPSGTILISDGLSNCVITLPKNSC
                     DLNLRVAGNRTITAQYSGNASYRSAQTSQNINVIAENKILLDIRPSLFPEKSIYTVGE
                     EITITAGLPDFLSDQPAATGSLFITDGISNCLLTLPKNNCELTIRAAGNQMITVNYSG
                     DEDYYPAQAKISIRAENQIPSATPSPQPTQTPIITPTIEPKNLSLKIAGVYPQKSSYH
                     IGDQIDIYIDPDYDALRSDFPKEKIRISDGYATCLFSVESQNHCSLQLIDPTTTKITA
                     AFPGDGFYLPVTSEPWIIKVEEPPKAKTTTSILNLNPVKDKYQIGDIVRVYAATEPDQ
                     SSEIPITGTITVSDSYSSCAILLPDQKFCDLTLSNGLAKEITAIFSGDDIYASSVSSV
                     TPIQISMVEVSAISVQAMQYSDCDHFMPDPNHVIPLKTDTSVVPEEHYFNLDEQFPIG
                     SGFYVNAIVRSKAGHLDTSPGTFNAKICSKEEPDSCVISDTVEAVRNQDDFTQAMGRL
                     EIPPIYRTGKHLLTITFTSDPELYGTETATFSLWNVQRGQLVLTPHSVLEPDPTYENY
                     LWNNLTATEKNSTDYLFDAYLLKGNNLRCPVALQSSLYEKPFGVTLQVEVDPVLPGAT
                     ITDSEWKAAILSNGYEAAMVDKLQPNSMLWDRNTCRWETNNENWFVHCSDVGISEPST
                     IRYALDYSDPNYIIPNRQNPADDFSAATIRVAIGKKLAFIHSKELLGALTPNTPYWIN
                     YEGSSFSWFASSTACDQEPYQGDYVSNSSIPADKIIHIHLLPLSRLALDSNFKWERIP
                     AEVKIDYTPAENPFAVLSTYHGICGNCSGKPLKKFDYIPNLGCVQTVAGQINLSPEEG
                     TLLAGTACPDGQPKMTGIQSTFCQLEFHTASEVHFSFSGNDDYQPADRQVALNISGIS
                     ASGSEQSVRLMSAPLMPNPSSEPQPVTILPTFTDSLGNVVYEHYVGEKYILRLQSSVP
                     LNENIMLIADLPDIFSTDSLDPDQSSCLKSIDFEEHQLRIAGSNFTGENNTFDCEIVF
                     KQPVSPGTIQTISFILENMNEKTNYRMTPFSWSGIPQSVVKKEVSIEAAVLNSNETIC
                     DNLNPFCGVMYPSESYQLKLKIALPNPPAENQKKVIFHIRWPEGTTIRNENSVPGSNC
                     EIGSDNQTPLEISNPSGETNLETSCVFSLNNLLLGPTNSIRISADSELYQIKATELEL
                     PTALSKRNITLIPSLQIRFDPSSDSESIEQIENQRIPRLYRTDLPDPNRQQNDAAAPA
                     FYTLNISVNGIPVEKSPASDETILVQWSLFDQLAAENDLPSCLIPLGDGDYQMTFHSD
                     WTAGCSFRIPMDFPINSAISPLRIQFLSPLYPTETTIPVAPEPFTVETAKAFITVPDK
                     ILLSENQQIAADLNPVSAPLSPYTAALIRRLRGDLLGIQWEYKQNLDCGDALNQSKDF
                     LYTCNFNFDRLPSANFESTVQLEVRPTGIEKLLEIQLLSAASGQPQNSFPFLPIEKIP
                     AELTVGPIYSPESPDIPLTSVPVGTNAQIDIGFNENSQPFSIENLIVTLEGTPLETCS
                     MNGNNSVRCTLPTSCSDLSENQTCERDLTITACYQGDEIHGSSQPVSISYRIVRYPIN
                     LEPSKNTVFQSDLLQHIEQYNEENGNWLPSSITIGNTTFDSYLVRETLSLSGQSEPAA
                     YPFFVNYSLNGFQSAQPLPENFWLDLTVGKSSGGGITEEIISIPPTSVNPNESEILFS
                     LDFGSNLQNTNGYQPGRQFDEVFEIRKIEVRYAGDTLIAPVSVSYQPEGFEFPIKLIS
                     MFEIRLSFQAESLDFGGTTPVPGIDQYTIFCSQPAAPLTCAADPTVPETGCWGNVTLN
                     LSMNPQVSAAPVFAPRCYLGGIRIDNGFDQIWLGDSLNQTFYP"
     gene            complement(7520..8545)
                     /locus_tag="PK898_02700"
     CDS             complement(7520..8545)
                     /locus_tag="PK898_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU18975.1"
                     /translation="MEKLKIGIAGLGRLGKIHAKNLADRISGCELTAACSLLESELEY
                     ARTELGVRDCYTNFREMLKNADMDAVAIVTTSGEHCNQLTAALNAGKHVFCEKPLGIS
                     VEECYLAEKAVENHPDKVFMLGFMRRFDPSYIYAKQKIESGTIGEPYLVKATGIDPLK
                     FIEGSLRFAGTSGGLFIDMAIHDIDLMRWYLGAEPKSVYAIGGSYGFPQFAALGDAEA
                     GCALFRFENGAMGTIHSGRTAAHGYHIETEIVGTKGAIRISPIPQKNLAMLYCEQGVV
                     TECVEAFPERFAEAYLSEMQEFVNCIREGRKPAVTVYDGTKATQIAFATTESFKNGRL
                     VEIPPHG"
     gene            8899..9846
                     /locus_tag="PK898_02705"
     CDS             8899..9846
                     /locus_tag="PK898_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU18976.1"
                     /translation="MKKFVLMFLIVIAATGVLVSAVGAEETYKIGYACNNFNDTFQTV
                     IEGYAKAYAKENGVEYVYSDAGEDILKQQDQIKAFIAEGVDALIVVPVDTSAAEPIID
                     MATEAKIPLVFVNRNPFAGVKPEDIPEGVYYVGSESIDAGIFQAEYLVKLLGTEKPVG
                     YAILVGMLSNEAAVARTEGNKQVLSKYPNYKLLAEKNGDWQRDQGLTITENWLTAFGD
                     ELNAILSNNDEMALGALEALEANGRDDVIVMGVDLIDDAKTAILEGRLDASVLQDGKG
                     QGEGAMKIALAALKGEKVDPITMIPYVLVTPENIKDYVE"
     gene            9952..11496
                     /locus_tag="PK898_02710"
     CDS             9952..11496
                     /locus_tag="PK898_02710"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU18977.1"
                     /translation="MDNDNYLLRMVNIDKSFPGVQALKSVNLNVGYGEVHALVGENGA
                     GKSTLMKCLIGIYSPNGGEIYFDGKKIENYTTAQALQMGISMIHQELSPILYRPIMEN
                     LWLGREPVNKFGFVDHNQMYKQSLDVLKEIELDEDPRTLMANLTVAKMQMVEIAKAIS
                     YNAKLIIMDEPSSALTEHEEEQLFKIIRKLKESGTSVIYISHRLSEIISIADKVTVMR
                     DGRDVGTENIENISLDQIITMMIGRTMDEMYPKLKVNIGEPILQVKNLSHRKFFKNVS
                     FEVRRGEIFGIAGLIGAGRTEVIETIFGIRPKSEGEILINGKPVEINSPRDAIANRMG
                     FLTEDRRATGIFSVLGVDVNMAIPNYDRFVTKFGFVNEKAVSESCMKFVNEIQIKTPS
                     LTQKIQNLSGGNQQKVLMARWLMTEPDILFLDEPTRGIDVGAKAEIYKLITMLAAEGK
                     CIVMVSSELPEILGMSDRIMVMHEGRVTAILDNGPDITQEVIMRYATGTENDFKEEYR
                     GIYAKE"
     gene            11483..12469
                     /locus_tag="PK898_02715"
     CDS             11483..12469
                     /locus_tag="PK898_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18978.1"
                     /translation="MQKNKFSFQNIFEKYGIVFVLILMIVLMSIAKPSFRTMNNFLNI
                     LTQSSIYGILALGMTLVIISKGIDLSVGSILGLSAVIAASLGQSMTASNKYFPDMGEL
                     PIIIPVIAALLVGSLCGAINGGLIAFTGIPAFIATLGMTTIARGFAYIYTDGKPISTL
                     TPAFKFIGQGKILGIPMPAVIYLIMIIITWVLLNNTRFGKRVYAVGGNINAAEVSGVN
                     VKKMLFLIYTYMGLLAGISAIVSTGRMVSAQPGLAAGYELTAIASTTIGGTSHSGGIG
                     TVWGAVVGALVLAVMRNGMTILGIHSYWQQIVEGLVIIIAVILDMRKNARKK"
     gene            complement(12534..13136)
                     /locus_tag="PK898_02720"
     CDS             complement(12534..13136)
                     /locus_tag="PK898_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18979.1"
                     /translation="MGTVITIQANVGLAPWEAFHMGLSQKTGISFGKIVILVGLLIVM
                     VSVILKEKIGFGTVLNMIFIGTFCDLIIDWNFIPRIDNYGLGILFMLFGLFVICLGSY
                     FYIGSALGAGPRDSLMIGLKKKFSGIPVGMIRGMIEGTVLLLGWLMGAKVGLGTVIAV
                     FGISFILQFTFKCLRFNVDDLHSESVTETVRNLGVIRKES"
     gene            complement(13281..13928)
                     /locus_tag="PK898_02725"
     CDS             complement(13281..13928)
                     /locus_tag="PK898_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18980.1"
                     /translation="MRRYLIRFIKLFLGLFLFSIGTVITIKANIGLAPWDAFHMGISI
                     QTGISFGKVVILTGVILLIVSVAMKEKFGFGTVANVLFIGIFCDFILSLNIIPTCESY
                     WLGIVFMLIGLFINSFGMYFYINAGFGAGPRDSIMIALNKRFPKIPVGAIRGSIEGIV
                     LFFGWLMGAKVGLGTILSVFGISFILQFTFRLMHFDVKKVKSESVLDTIQNIASI"
     gene            complement(14090..16696)
                     /locus_tag="PK898_02730"
     CDS             complement(14090..16696)
                     /locus_tag="PK898_02730"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOU18981.1"
                     /translation="MEHAKEKDHQPDKIYTPPMPEGLPSISLTENSIQVLTKRYVRRG
                     EDGKPIETIDEMFWRVAYHVALAEKNYGGDVIETAIAYYKLMVNKLFFPNSPTFTGAG
                     TPLGQLAACFVLPISDDMGKKSTGIFQTLRDAALIQQTGGGNGFSFSRLRPKGTIVRS
                     SAGQATGPVGFLRVYDHAFGEIAQGGSRRGANMAVLRIDHPDIEDFICCKTNENSITN
                     FNISVGITDDFMKAVEEDASWDLIYPDLTSPLTKEFDGDVQDAKDQGLPIKVYKTVRA
                     RDLFDLIVNQAHHNGEPGVLFIDQANRFNPVPNLYRLESTNPCGEQWLGPYENCCLGS
                     INLAQHVLEDGSVDWEKLEQSTVLATRFLDDVIDVNAYVPAVPQLKEAALRARRIGLG
                     IMGLADLMFNGGIRYGSEEGQEFASQVMEFIRYHSMKESIRLAEERGAFPAIKGSIYD
                     PDAIKWQIPTPLNPYRHDWNRPETDWEQIKEGMHRNGIRNAAQTTIAPTGTIATVTGC
                     EGYGCEPAFALAYVRHMNDNGKDVLLSYASPLFEKAMIKSGLDESTRKRLVDKVMEKG
                     SCQGIEEIPEEIRRTFVVSGDISAEEHIRMQASLQAFVDNSLSKTINFPEGTGPEEIA
                     KAYVLAWKLGCKGTTVYVTGSREKVVLETKKTAEEKQQIGMSEILPPTALKSEDSAQI
                     PLWYEVKKPRPRALRGFTYRAETPLGKAFVTINENGGNQPFEVFINTAKAGSETSAIS
                     EAIGRLISYTLRIASPLEPVRRLRDIQDQLRDIGGGRSLGFGANRVRSLPDGLAQVLA
                     EYLEEREERLLEQGTDVQPKKKAREDSSRRMAPTQNPMLRIGDLCPECGQASVINEEG
                     CRKCYSCGYSEC"
     gene            complement(16711..17163)
                     /gene="nrdR"
                     /locus_tag="PK898_02735"
     CDS             complement(16711..17163)
                     /gene="nrdR"
                     /locus_tag="PK898_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOU18982.1"
                     /translation="MNCPFCGEPKTRVIDTDKDEGNTVHRRRQCPACGKRFNTYERPL
                     LGIPMVVKADNSRQEFSREKLLRGIRIACAKRPVPAEKIEQLVDDIERELQMEGQREI
                     SSRVIGDKVIAGLKSLDQIAYVRYAIVYLQLDDLLSLRKEIDRLLSES"
     gene            complement(17526..17598)
                     /locus_tag="PK898_02740"
     tRNA            complement(17526..17598)
                     /locus_tag="PK898_02740"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17562..17564),aa:Glu,seq:ctc)
     gene            complement(17653..18846)
                     /locus_tag="PK898_02745"
     CDS             complement(17653..18846)
                     /locus_tag="PK898_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18983.1"
                     /translation="MDQKNKRIISLVILAVLILSSGLKLYRSPLDTYTWTELLINYAA
                     GFIRRGLIGRIGYLVSPYISVKFFLSALITICYLLHLIVYLRITRKIDFQDWLLFTFS
                     PVAFLFPIYNFDAYGRKDVFLVLIFMIVFLMVLKKVKWSILVPSTLILYEIGVLIHEY
                     ALFYFPFVVILIFIVYNNRPKRDLYLFSILSLAFLLGNLGWLYALSRRYYNLDLIPQS
                     WEGLIEDYHLTIQTGAFGWLGVPLKEGLQPVFDKLSYPQTFFSYLAGMALSAIPMVLL
                     NARYHILNKVSEFLEDRKGLLIFSVILLLSTMTIFIFSSDWGRLAYLYGFNIYLSLVV
                     LKEHFSCEDSVAGSTVLSGYQTVQWIAFFILFTGTWFLKMYVDGGNIALQQGLIFKIL
                     DWISI"
     gene            19023..20315
                     /locus_tag="PK898_02750"
     CDS             19023..20315
                     /locus_tag="PK898_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOU18984.1"
                     /translation="MAKNMYDFYKDAYSEIKGKMPERPYLDKQMSLPIYEGYSVANLP
                     ASICNWLGSENTGMLPPLAFEKIYGDRFRHVILVIVDGLGAYQMGMIRERLADNLWLR
                     LMSEYGTERLLTSIVPSATAAALTSLWTGVSGGTHGLVGYETWMRSLGMVVNFLQYSP
                     SSIGSKPGLIGDAGILPEAILPVPTLGNHLMGGGIISRSHMPIALAHSNLTRAQMAGV
                     SVVPYRRFGDLWENVKELLEADREKVTFDSVYWNDLDTYNHLMGMNDNRIFIDLGSFF
                     DGLARTVQALSRGNFGKTLVLVTADHGHIENTPDPNRDIKNHPELMHNLMIMPCGENR
                     LTYFYPKSGKDTAIRNYIEETWPDDFYLVSGQELIADGIFGTAAFHPDLDNRIGELVA
                     IARGYAYFWWPNRPDKLYSRHGGLSDQEMIVPLMGLAL"
     gene            20349..21644
                     /locus_tag="PK898_02755"
     CDS             20349..21644
                     /locus_tag="PK898_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOU18985.1"
                     /translation="MTIDRIAVIGQGYVGLPLALSFALHGCHVIGVDSDPDVCSNLKK
                     GITAQTETYQGQSIQTILQTMLQKGTYQITDNGAEAVRRSEVIVLTVGIPIESGVPVY
                     EYFEAACNTIGENLQKGSLILIRSTVIPGTTENFCKPIFEKKSGMKAGVDFFFAYVPE
                     RIAEGKAFEEFETMPTLIGAPDEKSFDKAAAIIRMISKADIIRSDSVMAVETSKVLEN
                     LQRDANIAIVQEFARFAEAAGMDIMEIIRLANTHKRVNLLTPGPGVGGYCIPNAFHYL
                     NVKADELGIELPLLKLARQQNDRLPQFFADKTEELLQAAGKSIAGAKIAVAGLAMKDF
                     SPDDRLSPAVMICNLLIRSGAKVAAYDKNVATRHPFKVDSFREAANSADALLILNRIS
                     RDFNPLEWIDEMKKPIVILDTRGIIEPEDLPEGTVFWRI"
     gene            21780..22826
                     /gene="hrcA"
                     /locus_tag="PK898_02760"
     CDS             21780..22826
                     /gene="hrcA"
                     /locus_tag="PK898_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOU18986.1"
                     /translation="METLTERQKLILSLVVHDYTKTARPVGSKRLVEKFRLDFSPATV
                     RNELAALTEMNFLRQPHTSAGRVPTEKGYRYFVGTLMQTQELSEENRRMIEHQFFQTQ
                     QEQGIDGWMKLAASILAVQSNAISVVSAPMPVRNGLKHVAVIGITGKQILLVLVLDGG
                     QMQQRFITLDEQTSQNELTVIANEINQRFKGMTTEQIRKESQIEKSRDPLMELILKNV
                     LNAMYETDAMKAGEVYTDGISNVLAEPEFSESEDARRALKILEERPALQNFLSRTTWD
                     QKIGGVQVLIGGEGLLANLRNCSMVLARYGLPDTLTGTVGVIGPMRMPYSRNIPTVRF
                     IAGLLSDLVSESLG"
     gene            22874..23503
                     /locus_tag="PK898_02765"
     CDS             22874..23503
                     /locus_tag="PK898_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOU18987.1"
                     /translation="MAKKSRDQKDNREIPEKEERAENTEINDENVGVDKNPDFSAPDG
                     CEEVLVLSKAEVDEMKEKLHRSQQKEKENLESWQRERADFINYKKRIDREQEILQQNY
                     KAELLKKYLVILDDLELAMRNKPEEGEDPTNWIRGIELILRKFESINQNEGLKKIEAE
                     GQEFDPRYHQAISHEEDPNVESGKIIEVLQNGYMLGDRVIRPALVRVAK"
     gene            23515..25434
                     /gene="dnaK"
                     /locus_tag="PK898_02770"
     CDS             23515..25434
                     /gene="dnaK"
                     /locus_tag="PK898_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOU18988.1"
                     /translation="MGKIIGIDLGTTNSVMAVMVGGEPTVIPTAEGERICPSVVAVNK
                     NHERLVGRVARNQAITNPENTIFSIKRFMGRKYDDPEVQKTLSRIPYKVTKAPNGDVR
                     VVLDGKEYSAPEISAMILAKMKADAEAYLGEKITQAVITVPAYFNDSQRNATKDAGKI
                     AGLEVLRIINEPTASSLAYGLDKKTNELIAVYDLGGGTFDISILDVGDGVFQVRSTSG
                     DTFLGGDDFDQRIMDYLIDEFKKANNMDLRQDRQALQRLKEAAEKAKIELSSTMQTEI
                     NLPYITADATGPKHLVMTLTRAKVEQLTSDLIARSLEPVRNALRDANLKTSDINEVVL
                     VGGMTRMPAVQEAVKREFGKEPNKSVNPDEVVAVGAAIQAGVLGGEVNDILLLDVTPL
                     TLSVETMGGVATPMIERNTTIPAKKTQIYSTAADSQTSVEIHVLQGERPMAADNKSLG
                     KFILDGIPPARRGVPQIEVTFDIDANGILKVTAVDKATNRSQNITISASSGLTDAEVE
                     RMRKEAEIHAEEDRKRKEGIEIKNNADNAVYTAEKLLEENGDKIPADMKSNIQSKAGE
                     LKNILNGTDFDTMKRMTDELNKMIQDIGNIMYQQQQPGAGQSQSDQGSGASGQNPPTG
                     NDGNDDVVDGEFRSM"
     gene            25569..26717
                     /gene="dnaJ"
                     /locus_tag="PK898_02775"
     CDS             25569..26717
                     /gene="dnaJ"
                     /locus_tag="PK898_02775"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU18989.1"
                     /translation="MVKDRDYYEVLGVGRNASSDELKTAFRNLARKYHPDINKDPGAE
                     ETFKEINEAYQVLSDPDKRAAYDRYGKAGVSGVGNGFDYSQVDLSDILGDLFGFGGFG
                     GFGGFGSTQYSSRQRNAPRRGADLQTTVTLKFEEACFGVDKEVQFSRDEKCSRCHGTG
                     AEPGTSPQRCYTCNGTGEIKTNRQTMFGSMVQVSTCPTCNGRGEIISTPCTQCHGRGL
                     ERKTVKKVVSIPAGVDTGTRIRLSGEGQPGDNNGPSGDLYVDIRVNPHKYFHRKDADI
                     QLDMNINIAQATLGDEIDVPTIDGTEKLKIPAGTQPGRVFTLKGKGIPRLQGSGRGDQ
                     KVVINVEIPNSLTPEQRDLMEQLAGTMGTEVIPQEKSFWDRLKDALNG"
     gene            26710..27633
                     /gene="prmA"
                     /locus_tag="PK898_02780"
     CDS             26710..27633
                     /gene="prmA"
                     /locus_tag="PK898_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOU18990.1"
                     /translation="MADPAWLEISVVVDSETAEAVAEVLSRYAENGVVVEQGVDYNDA
                     EDVGTPFGPCRVTGYLPVDETTEEKRQRILEGLWYLGRITAVPEPEFKFIEDEDWMAA
                     WKKFYHPILIGEKLLILPAWMPSIEPERIAIKIDPSMAFGTGTHPTTQLCLAMVEKYI
                     VPNEDIIDMGCGSGILSIGALLLGARHALGVDIDNESMQSSRENAENNGVADRLELFK
                     GTVQEILNGACSIRQAPLVTANILTPILIQLFDQGMADLITPGGVILLSGILEEQDEK
                     IRNAAEAQGLEFLEKRQIADWVAYAYRKPQK"
     gene            27910..29175
                     /locus_tag="PK898_02785"
     CDS             27910..29175
                     /locus_tag="PK898_02785"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOU18991.1"
                     /translation="MINSIRERIKQLEHQRSMSGLEILKYIGPGLLVTVGFIDPGNWA
                     SNVAAGSDYGYKLLWMVTLSTFMLIILQHNAAHLGIATGYCLSEASSIYVKPWLSRFL
                     LTTALLATAATAMAEILGGAIALQMLFNLPLKIGAILVTVLSLTLLLTNSYKLLERWI
                     IGFVSLIGLSFIIEIILVDVDWGQAAVSWVKPSIPAGSIPVIMSVLGAVVMPHNLYLH
                     SEIIQSRQWNLKDDEVIRHQLNYEFLDTIVSMLIGWAINSAMILVAAATFFANSIHVS
                     ALDTAGAMLKPLLGNGASVIFAIALLLAGISSSITAGMAGGTIFAGMFKEHYDIHDIH
                     SRGGVIAIFLLALVVIFFIRDPFMGLVYSQMLLSIQLPWTIFLQIYLTSSQKVMGKFA
                     NSKGNFILLMIIGLIVTGLNIVLLISFLR"
     gene            complement(29265..>29522)
                     /locus_tag="PK898_02790"
     CDS             complement(29265..>29522)
                     /locus_tag="PK898_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18992.1"
                     /translation="GRLSISDIVATAELPEEIKKDPDLLTGCIAGAEQADTIRDLLQR
                     VGFKNIRLTPKDNSKEILKSWVPGSNVEDYVASFLIEAVKE"
     assembly_gap    29523..29622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29623..29843)
                     /gene="arsM"
                     /locus_tag="PK898_02795"
                     /pseudo
     CDS             complement(29623..29843)
                     /gene="arsM"
                     /locus_tag="PK898_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986176.1"
                     /note="in Rhodopseudomonas palustris this protein confers
                     resistance to arsenite; catalyzes the formation of a
                     number of methylated intermediates from arsenite and SAM
                     producing trimethylarsine; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
     gene            complement(29983..31071)
                     /gene="arsB"
                     /locus_tag="PK898_02800"
     CDS             complement(29983..31071)
                     /gene="arsB"
                     /locus_tag="PK898_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOU18993.1"
                     /translation="MNTKSENIGIGFFQKYLSIWVILCMVAGILIGKFLPGIPAFFGR
                     FEYANISLPTAILIWVMIYPMMLKVDFQSIKNVGKNPKGLYITWITNWFIKPFTMFAI
                     GSFFLYVVFKNIIPADLGKQYLAGMVLLGAAPCTAMVFVWSTLTKGDPAYTVIQVATN
                     DLIILVAFVPIVKFLLGISNVTVPWDTLVLSVLLFVVIPLAGGFLTRVTVIKNKGVDY
                     FENIFLHKFDQVTIIGLLLTLILLFSFQGQTILRNPLHIVLIAIPLIIQTFLIFFIAY
                     FACRWAKLPYSIAAPAGMIGASNFFELAVAVAIALFGMDSPAALATTVGVLTEVPTML
                     ILVNIANNTRHWFPALEISSKKASEKSY"
     gene            complement(31091..31411)
                     /locus_tag="PK898_02805"
     CDS             complement(31091..31411)
                     /locus_tag="PK898_02805"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU18994.1"
                     /translation="MDDVYREYAPIFKALSDETRLKIVDMLSCGEMCACDILTEFNIS
                     QPTLSYHMKILSDSGLVFAKRDGAWMKYTINQEKSNTLIDFLSRLMKEKDDCICKKYQ
                     QNSK"
     gene            complement(31542..32216)
                     /locus_tag="PK898_02810"
     CDS             complement(31542..32216)
                     /locus_tag="PK898_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOU18995.1"
                     /translation="MLIGVIVNAAAVAAGGILGSVLHKGIPEKMNQLMMQGLSLCVLY
                     VGISGSLEGNNTIVVVLSVVFGAVIGELFNLDGKLQSFGDFLQSKVKSDNPISEGFIT
                     CSLFVCVGAMAIVGSLQSGLTGNNETLFTKGLIDGIIAMIMASTMGIGVAFAAVPLFI
                     YQGILTVSAGAISGYLTMNVINEITCTGSLLIIAIAFNMLKLTNIKTANLLLAPFLPI
                     LFCRFF"
     gene            complement(32251..33174)
                     /locus_tag="PK898_02815"
     CDS             complement(32251..33174)
                     /locus_tag="PK898_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU18996.1"
                     /translation="MIMEIVFWQMLTLFIIIVVGLICGRTRVITESETKGISGLIINV
                     FNPALVFSSVINNRGRVQDNMLQSITIIAIAMFAGLILIGIVTAGFFSKDKDQQIMYK
                     LMLAFPNVGYIGIPLINSIYGPSASIYVAIFILLFNIVIYTYGIALLKGKTGKRLSVE
                     NLKSILNMGTISCLITLLFFIFGIPVPDIVKTPITYLGNCATPLALMSVGFSLANAKV
                     SDVFMDKRLYIFSAFKLLLLPAIGVFLLKLFPLPEQVIGISAIMFAMPTGNLPVILAN
                     RYGVNSDICSSAIIITTLLSVLTIPLIAGMI"
     gene            complement(33373..35163)
                     /gene="aspS"
                     /locus_tag="PK898_02820"
     CDS             complement(33373..35163)
                     /gene="aspS"
                     /locus_tag="PK898_02820"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOU18997.1"
                     /translation="MYKTHTCGELRISDVGKTVTLAGWVHRYRDHGGIYFIDLRDRFG
                     LTQVVINPEDSPELSKLVADVRVEWVLQVIGEVRARPADAKNPNLDTGDIEVYATEIN
                     VLNTCKPLPFSINKEENTDETLRLKYRFLDLRRERMQKNLILRHKIIKYMRDYLDAKD
                     FLEIETPILFKTTPEGARDYLVPSRIYPGEFYALPQSPQQLKQLLQVAGVERYFQIAR
                     CFRDEDQRGDRQPEFTQLDLEMSFVERDDILDLMEDLFTKMCEDLVPQKKILFSPWPK
                     LTYQEAMDRFGKDSPDLRFGMELVNISDLAKDCGFTVFESAIKDGGSVRGINAKTLGE
                     YTRKQLDELTDQVKLWGGKGLAYVKISESGEESSPFKKFFSNEKWEELLKRMEAEKGD
                     LLLFVADSKPAVVYDCLARLRVMLGDRLGLRDNNKLAFCWIVDFPLFEWSEEEQRWDA
                     THHPFTRPKKEDFDLLDTDPGKVRGDQYDMVLNNYELASGSIRIHERWLQEKVFDLIG
                     LPRDVARDRFHQIMDAFEYGAPPHGGIAPGIDRFVMLMADEPNIREVIAFPKNNAARD
                     IMSGAPSPAMEGQLRELHIKVVYPEEEKKS"
     gene            complement(35420..36472)
                     /locus_tag="PK898_02825"
     CDS             complement(35420..36472)
                     /locus_tag="PK898_02825"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU18998.1"
                     /translation="MKKTILGIIILILILTGCQSEGNKTPAVTLSAEQVQLWAQQTIQ
                     AMVSSNTTKTAVVLIPQASPTATPTVIRPTIAIPTIVISQPTARPVIPTISYVQPVQK
                     VCDQMGFVADVTVPDNTIMQPNQVFQKVWRIQNTGTCTWTTSYQFVFDSGYQLGAPSA
                     INLPRNVLPNETIDISVDMQAPSVNGTYQGFWKMRNPSGIKFGTAATQSGNDSVWVKI
                     IVNQGADSGFVPTSYPGYIGNGTCSIISMFPTSADSFYAGQETDFTVTVRNESDTVWT
                     ASDFDIAFIGGTNMLKRPDGIRFDLKSDVYPGQTLTFGLDIITPSGYGTYTMEWGVVR
                     NNEILCPIYFTANVNK"
     regulatory      36740..36868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            36917..37693
                     /locus_tag="PK898_02830"
     CDS             36917..37693
                     /locus_tag="PK898_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU18999.1"
                     /translation="MQKYGNKGNTLRHLIPPISICAGLCIWEILVRIFNYPPFILPAP
                     SRIFSRLIESIASGVLLYHTGITLVEVLTGSITGTILALLTGYLLAHHPIAENVLFPY
                     IVASQSIPMAAIAPLLIIWFGSGIMPKILICALVVFFPIQINVIIGLRDIQRNFLDLM
                     KSMHANRKAQLKYLEIPAALPVVFGGLRISATLSVIGAVVGELTGADAGLGYLINTAR
                     GQYDTAMVFVAVFMLMIISILLYVLILAIEKRTLRWQTVQ"
     gene            37714..38700
                     /locus_tag="PK898_02835"
     CDS             37714..38700
                     /locus_tag="PK898_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19000.1"
                     /translation="MIYKTIKKLLSVLLTVLFLMTGSNRVFAEEFSLPVGFVPNVQFA
                     PLYVALENGYFAGENLKITLDHSLETDTVALVGANKIPFGICSGEQVLLARNQGLPLT
                     YIAEWYEQYPVGIVSLKEAGITSMQDLRGKKVGVPVLSGASYIGLEALLQQSNMTDAD
                     IQLETVGYTQVELLISGKIDAAVIYIANEPEQLKALGYEINLIRVADTVPMVSNGLIT
                     NEKTIRENPELVRRMTRAFLKGIQWTRQNPDQAYEICKKYVDNLANAENQELQKQILI
                     QSIALYQSPSSETPLGTTDAAAWEKMVEVMKQMKLLKPDFDMSGSFTNEFLP"
     gene            38724..39470
                     /locus_tag="PK898_02840"
     CDS             38724..39470
                     /locus_tag="PK898_02840"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19001.1"
                     /translation="MIASIENLGMTFPSENGGLQALSDINLTLYDREFVCIIGPSGSG
                     KSTLLRILADVLVPTQGKVTFADGQNPNRAIVFQSSNLLPWMNVIQNIALPLKIRGAG
                     EKEALSQAGNWIRRVGLNGFEYEWPQNLSGGMKQRVAIARALVQQPELLLLDEPFGSL
                     DALTRDQMGLELLKTWETFHPTVLMVTHSITEAVLLADRVAVFSKRPGQIIHELEIPF
                     QRPRSPELRDEIKFLQVVKKLRGMIVTESI"
     gene            complement(39506..40504)
                     /locus_tag="PK898_02845"
     CDS             complement(39506..40504)
                     /locus_tag="PK898_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU19002.1"
                     /translation="MSWDIFGHDQVVNILRNHILQDEVRHAYLFVGIPGTGRRTIALQ
                     FAQALNCSEPPAPGEFCGKCRNCRQIAAQQHPDLIVTETETAGTILKIDEIRDLQHTL
                     SLAPYESRWRIGLLLRFDEANQNAQNALLKILEEPPENVKLLLTADSENSLLPTITSR
                     CEVMHLFPANPAQLTSYLTDRYQVESKAAESAAHLSAGRVGFAISLLKEPEKAEQMNR
                     TAAECLEMFSQNTRERFRYASNFKDFKKRTELREVLHVWQSVMRDLLLLSTGGKLTAQ
                     SLTFIDLCSESASVAAKYSTEQFRELIRHLNRSLEYLDANVSPQLLLEELLLNFPS"
     gene            complement(40501..44667)
                     /locus_tag="PK898_02850"
     CDS             complement(40501..44667)
                     /locus_tag="PK898_02850"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19003.1"
                     /translation="MFTKLKTALGLDPIQKKLEEYTAIVSEINRLEPDYEKLSDEALR
                     AKTDEFKDRLANGETLDDILTEAFATVREASKRVLGQRPYDVQLIGGIALHKRNIAEM
                     KTGEGKTLVATLPVYLNALAGKGVHLVTVNDYLARRDARWMGRIYQALGLTVGVLQMA
                     ARTDNGRKAFLVDYSIESPHEDQDHLRLVPRKEAYDADVTYGTNSEFGFDYLRDNMVM
                     KLEDRVQRGHSYAVIDEVDNVLIDEARTPLIISGPASDDVDAYYQMAKVVKQLSPDEY
                     EVSERDHNVYLNDNGMDHVEELLGMVLRDPSRPEEVTIEQERMMGYLNQALAAEFLYH
                     RNKEYLVQSGKVIIIDSSTGRLMPGRRWSNGLHQAIEAKEGVKVEPENMTYATITLQN
                     YYRMYDKIAGMTGTALTEKEEFYKIYLLDVIPIPTNLEYTARQKNSNLIEREAKDEEG
                     YKYTYYADPAAPDIPLYYKRKDYPDVIYQSEEAKFRAITIEILKNHVIGRPQLVGTAS
                     VEHSEYLASRLKQEPLRRLVQILMLRRAWMKQNNIEVLESPLKEFIPFNKPIQEINAG
                     DLRPMAKQLGVSLNVDDPDNRSLLMEEFGLSESNVDRFIEVVESGMNPQVLNARKHDE
                     EGMIIAKAGALGAITIATNMAGRGVDIKLGGELDEERIRDTNRVLTKMGIDPYNMTLD
                     ERYQAILKVPPEEYGVYEESVKAYIDYIDQMEKVRDLGGLHVIGSERHESRRIDNQLR
                     GRAARQGDPGSSRFFLSLQDEIVRLFGGEQLEGVLKRVNLLDVNVPLENNLFSRMIEQ
                     SQERVEGANFDARKHTLEYDDVLNSQRKRIYEQRDQAFVKEDLSEDVHAMLETDLDNR
                     LDKAMDEEKWKLALYLDSIQPTIEVEENYLPSFSQSLLIQSLKEKVGSAPEKENLLNA
                     LDELSREAFRRENEVGLEQMETLIRNSQSGYESQLEERTANFELFVDSLKERLKEQQE
                     AKEEGRVVEPIRPQDLLTEAGNIARVGFKLSPDKLRKLAEGDADIIGELRSQIEIALF
                     AGYIQRLNQLIGNRMIGDYEPPTTKFEIGDWEGFENAVMDAVQKAFRTRAERLFGNQG
                     QVKSDLESALRTYQPAELTDKQWVQLFRTISQGQRIGFDAKTHRKTSRIFTRINLVYT
                     AGDLLKDLDREELKELIWNHYMNAEDGLRQIFGKLDWGRLRSYNIPLSQLKPETQEKI
                     SRAIGSEIFEEVKEIPPAEMDGEIAAEIQSYLGKRIQNEVNRQVILRSITGQWVDYLT
                     QIEGLRVSISMESYAQRNPLVVYKTKAAELFSQLLNDIRRSVVEQMFVTLPQLSMLTA
                     AERISLADLRQQEPSPQESNAEPVESDSDRSDDGQISPDETAQKEEPREREVVQASSP
                     SKKKKKKMKK"
     gene            complement(44912..45661)
                     /locus_tag="PK898_02855"
     CDS             complement(44912..45661)
                     /locus_tag="PK898_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU19004.1"
                     /translation="MNQTITHQLNHRSIREFTDEPVKPEDLKLVLEAANHTATSTGMQ
                     SAAMIRVTDMAKKQALSEICQQPYVARFPELIIFIVDVYRNARIAEDNGYQFESERDM
                     DRFFQGWTDACLMAQNATNAIESLGMGAVFLGSILNDSAATIQVLKLPKLTFPVLGVG
                     FGWPNQNPQLKPRLPLDLRVFENEYKILADYKEAVREYDEEMQTYYDLREANRRVDSF
                     TLQVIKKLQNPSEKRAKILQVVRDQGFDLKV"
     gene            complement(45677..46543)
                     /locus_tag="PK898_02860"
     CDS             complement(45677..46543)
                     /locus_tag="PK898_02860"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU19005.1"
                     /translation="MEKRRLGRTEMMISPVVYGGIIHMGETQETANRYVSYAVDHGVN
                     YFDVAPSYDDAEQLLGVALKPYRKNVFLACKTMERTASGSLRELENSLKTLQTDHFDV
                     YQLHSITTAEDVDTIFGPDGAMETFLSAKKKGIILNIGFSAHSEEQALRAMDLFDFDT
                     VLFPMNWALGLVRGWGDRVAQKVRESDKGLLCIKTLVDRKWREGENRDEFPKSWCHPT
                     TDNEKTGMAGMKYAFSKGATALVPPGDFKSFNFMLDHIDRVLQNPLNEMDWIYLQEEA
                     QKIKDELIFDNQ"
     gene            complement(46719..48452)
                     /locus_tag="PK898_02865"
     CDS             complement(46719..48452)
                     /locus_tag="PK898_02865"
                     /inference="COORDINATES: protein motif:HMM:NF023203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19006.1"
                     /translation="MIKTELQENRRASNIIWNAAFDYSFRPEVKYFDQNGKAELYGNY
                     IIGGAHYYYDFPLLEEFFEKLKQDADADFLRGLTWLGLENSTFLKGRKDRPVLEELRR
                     NFANTILENYSVTADSDILDDLKVAHFQRVLGKQPVMSETVANVLDQLEFDESLTTEQ
                     IIEKLNAVILNYFQFQKAEYEKNRLAFLQKNKTVYQWNETNRKRFLRIPFLRNPKNEP
                     LEFPLATNSDRVPVRKNILTSYWRKYRNANEINQEKYIREYYGKPVLSDSEVKILEKK
                     LCTGAHKNCRLHFTRGEIDVSGNLSEQQSVILMQREKNRAYFRKNLAVNNTGIIKLTE
                     KIRNSMLLMPSSFSGRANAGRLTPERAWRNLYIHDKNVFQKKIQNEIGDLSVDILLDA
                     SASQLGRQEAIATQGFIIAESLTRCQIPVRVYSFCTKRKFTIMTLFRDYDEIYDNDKI
                     FNYFSSGCNRDGLAIRTAIHMMKNSPYQHKLLIILSDAKPLDTNIVPTTGKTQVEADY
                     TDLVGLNDTAAEVRKSRDFGISIMCVFTGMDEDLPSAKRIYGHNFVRIKGLERFADSV
                     GILIQNELKWL"
     gene            complement(48452..49366)
                     /locus_tag="PK898_02870"
     CDS             complement(48452..49366)
                     /locus_tag="PK898_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOU19007.1"
                     /translation="MLDFLRKEGIRSDLISQIELFREKYPVSPEKKYRIPQPKYYYYG
                     NDIWEEAITALLCGENLLLVGPKATGKNVFAENLAAAFGRPSWDVSFHINTDSSSLIG
                     IDTLEEGRVLLRHGPIYECARWGGFGILDEINMAKNEAMAVLHASLDFRRIIDVPGYD
                     KFILADSTRFIATMNYGYAGTRELNEALTSRFMVIEMPVISQKNLKRLLLREFPTINE
                     KYIEQFSGLFQDLQKKSENSEISTKPVDLRGLLAAIHLMDRGLEADKALQMGITNKTF
                     DDFERKLVEDVIRLRIPAGLERDAIFKD"
BASE COUNT        14054 a        11487 c        10540 g        13330 t
ORIGIN      
        1 aaaaataatt cagggttttc ctgtttagtc gctgaaattt gccgattttg acataggaca
       61 atttgaattt cgctgctata ataaaaaagt ttgtttgctt tctacatcaa aatattttat
      121 aaaggacaga catgaatctg aaaatcatca atcaagatgg ttgggaaaag aacgtgtcaa
      181 tcgccaaaac ggttatcttg ctcggtcccc aagcaggcaa cgacattttt cttccgatta
      241 aagagcaatt tcaaatcatt cattccggtc ccgaaaaatc cgatattaaa attttccgtt
      301 tcaactccac ttcttcaagt gaagattttt tcatttttcg aaatggagaa agtatttccg
      361 tactccctct agaggaaaca gaattgtttc cgggtgatcg gatcctaatc ggaaattatc
      421 aaatcgaatt cgaagggaca acgattgata aaccgtttga accgcaaaat ttggaacaac
      481 aatcaaaaag aaggacgaat tattggatat ggatcgccct tctttttatt ctcttatttg
      541 ctgtaggata ttttctgcag ccgaaaatta taaaattcgt ttccgatcag cccacaaata
      601 ccttggtacg cggagattcg gcggaattga gttgggaagt ttcaccgttc gttacgcatt
      661 tgacgatcac atctgatgaa gaaaccctac cggtttcaaa gacggaaaga tttcgagttt
      721 ctcccgaaaa aacaacaacc tatacactta ccgccgagaa ccggattaca aggattctcc
      781 accgccccca tcgagcggaa ctggttttgg cggtaaatca accggaatcc gaaagcactt
      841 tgaaaagtga aaaaatttcc aaaaataaat ataagctgga atgggaagta acgagtacca
      901 ctcgaatggc aaccctcgtc atcggtgaaa atcgtcagga attgaactct gatcagtatc
      961 acggaagtca aaccgttaag attcaggaag acaccccggt ccagttaatt tccgaaagtg
     1021 aaatgggaaa agaagttaaa aatttggttt tgaaagctga aaatccgcaa atggaattgg
     1081 tcaaattcat catttggatt cgcccctctg ctcccaaaac gaacacgtcg ccggttcaac
     1141 ggatagacaa ttcggatgga tcttccgacg aatttaccgc caaatatgcc gagctggttc
     1201 ccgatccgag cagtttaacg ggttataaaa tcgttcgata tgaatttgac cggcaaatta
     1261 tttccggcga acagattatg ctcgaatggg aagtgaagga tgccgaaacc gttctgctgg
     1321 aacctttatc ggatcaattt gtttctcaga aaggggctca attctatttt ccgacccaat
     1381 caacgaattt caccctctcg gtttcagatg gaagccggct gaaatcttat accttgccca
     1441 tttccatccg cacggaaaag aatgtcgagg ctgtcgcacc aaaaatcgat cttttcgatg
     1501 ccaatcctgt tactctcacg cagccgggaa agacaacatt atcttggtca gtctccggag
     1561 attggacccg tattcaactt attgcgtccg gtgcctcctt cgattccatt catgccgaca
     1621 caaataatga aaatgccatt cctttaacga tttctcaaaa cagtggatcc attctcgccg
     1681 attatctcgc accgaccggg tttctttctt tgaacctaaa agaaaacgcc tcttttattt
     1741 tgaaagcttg gagtgggaat ctttcaaaca gttcgattgt caatgtccgg gtaaatccat
     1801 cggaacctcc cgccgtatta aaatcaccgg acctgaaaat taacgcaatt ttcaccgatt
     1861 cagacaatta tacggtcgga gacacaataa cagtgagcac cgctttcgat gggctatcct
     1921 caatagactc tattccaagc ggcacaattt taatcagtga cggactatct aactgcgtca
     1981 ttacgctgcc gaagaacagt tgtgatttga atttacgtgt cgccggaaat cgaacgatca
     2041 ccgctcaata ttccggaaat gccagttacc ggtcagccca aacctctcaa aatattaatg
     2101 taatcgctga aaataaaatt cttctggata tccgaccttc tctatttccg gagaaatcga
     2161 tctataccgt cggagaagag attaccatca ccgccggcct tcctgatttt ctatccgatc
     2221 agcctgccgc gaccggatca ttgtttataa ccgatggaat ctccaattgc ttgttgacgc
     2281 ttcctaaaaa caattgtgaa ttaacgattc gcgctgccgg gaatcaaatg atcactgtca
     2341 attattccgg agatgaagat tattaccctg ctcaagcgaa aatcagcatt cgtgccgaaa
     2401 atcaaattcc ttccgcaaca ccgagtccgc aaccgactca gacaccgatc attacgccga
     2461 ctattgaacc gaaaaacctc tcattgaaaa tcgccggtgt ttatccacag aaaagctctt
     2521 accatatcgg tgatcaaata gatatttata ttgatccgga ttatgatgcg ttaagatccg
     2581 attttccgaa agaaaaaatc aggatcagcg acggttatgc cacttgtctt ttttctgtgg
     2641 aaagccaaaa tcattgttcg cttcaattga tcgatccgac cactacaaaa atcaccgcag
     2701 cattcccggg agacggattc tatctccccg taacatcaga gccttggatc atcaaagtgg
     2761 aagaacctcc gaaagccaaa acgaccactt caattctcaa tttaaatccg gtcaaggata
     2821 aataccaaat cggcgatatc gttcgagtct atgccgcaac cgagcccgat caatcctcgg
     2881 aaatcccgat cacgggtacg attactgtca gcgattcata cagttcttgc gccattttat
     2941 tacccgacca aaaattttgc gatttgacac tttcaaatgg acttgcgaag gaaattacgg
     3001 cgatcttttc gggtgatgat atctatgcat cttccgtctc atccgttacc ccgattcaaa
     3061 tttccatggt cgaagtttcg gcaatctccg ttcaggccat gcagtattcc gattgtgacc
     3121 atttcatgcc tgatccaaat catgtgatcc ctctgaaaac ggacacttct gttgtacccg
     3181 aagaacacta ttttaatctg gacgaacaat ttccgatcgg cagtggtttt tatgtaaatg
     3241 caatcgttcg ttcaaaagcc ggtcacctcg atacctctcc cggaactttc aatgcaaaaa
     3301 tttgcagtaa agaagaaccg gattcctgcg taatttccga cacggttgaa gccgtacgaa
     3361 atcaagatga ttttacacag gcaatgggaa ggcttgaaat ccccccaata tatcgtacgg
     3421 gaaaacatct cctgaccatc acttttacga gtgatcccga actttacggt acggaaacag
     3481 ccactttcag cctctggaat gtccaaagag gtcaattggt actgacgcct cattccgtac
     3541 tggagccgga tccaacctat gaaaattacc tgtggaacaa cctgacggcg acggagaaga
     3601 attccacgga ttatttattc gatgcatatt tactgaaagg aaataacctc cgatgcccgg
     3661 ttgcccttca atcttcgttg tatgaaaaac ctttcggcgt caccttgcag gttgaagtag
     3721 atcctgtcct tcccggtgca acaataaccg attcggaatg gaaagcagcg attctttcaa
     3781 atggctatga agccgcaatg gtggacaaac tgcagccgaa cagcatgctt tgggacagaa
     3841 atacctgtcg ctgggaaaca aataacgaga actggtttgt gcattgcagt gatgtcggaa
     3901 tctccgaacc gtcaacgatt cgttatgctt tggattattc ggacccgaat tacataatcc
     3961 cgaaccgaca aaatccggcg gatgattttt ctgctgctac gatacgagtc gctatcggga
     4021 aaaaattggc ttttattcat tccaaggagc tattgggagc attaactcca aatactccct
     4081 attggatcaa ttatgaagga tcaagcttca gttggttcgc atcaagcaca gcatgcgatc
     4141 aggaacctta ccaaggcgac tatgtttcca attcatctat tccagccgac aagataattc
     4201 acattcatct tttgcccctc tctcgattgg cattggattc aaatttcaaa tgggagagaa
     4261 ttcccgcgga agtgaaaatt gattacacgc ccgcagaaaa tccattcgct gttttgagta
     4321 cttatcatgg tatttgcgga aattgttccg gaaaaccgtt gaaaaaattt gattatattc
     4381 ccaatctggg atgcgttcaa acagtagccg gtcaaatcaa tctatctccg gaagaaggca
     4441 ccttgctcgc cggaacagcc tgcccggacg gacaacccaa aatgacggga attcagtcaa
     4501 cgttttgcca gcttgagttt cacaccgcat ccgaagtcca cttctctttt tcgggaaacg
     4561 atgactatca accggccgat cgacaagtcg ccctcaacat aagtggaatt tccgcgagcg
     4621 gttccgaaca atccgtccga ttgatgtctg cccccttgat gccaaatcct tcttccgaac
     4681 cgcagccggt aacgatcctt cccacattca ccgatagtct cggaaatgtg gtttatgagc
     4741 attacgtggg cgaaaaatat atcctccgat tgcaatcttc cgtaccgttg aatgagaata
     4801 tcatgctgat tgccgacctt cccgatatct tttcaacaga ttcgcttgat ccggatcaaa
     4861 gcagctgcct aaaatcgata gacttcgaag aacatcagct taggatcgcg ggatcgaatt
     4921 tcacgggaga gaacaatact ttcgactgtg agatcgtctt caaacaaccg gtttctcccg
     4981 gaacaatcca gaccatttcc ttcatcctcg aaaatatgaa tgagaaaaca aattatcgga
     5041 tgactccgtt cagttggagc ggcattcctc aatccgtggt caagaaagag gtatcaatcg
     5101 aagcagctgt cttaaacagc aacgaaacta tttgcgataa tttaaacccc ttttgcggag
     5161 taatgtatcc ttctgaaagt tatcagttaa agctgaaaat agctcttccg aaccctcccg
     5221 cggaaaatca gaaaaaagtt attttccata tccgatggcc cgaaggaaca accatccgga
     5281 acgaaaattc agttcccggc tcgaattgcg aaatcggttc agacaaccaa acacctttgg
     5341 aaatttcaaa tccatcggga gaaacaaatc ttgagacttc ctgcgttttc agtctgaata
     5401 atctgttact cggaccaaca aacagcattc gaatttccgc cgattcggaa ctgtatcaaa
     5461 taaaagcgac agaattggaa ctcccgacgg ctctttcaaa acgcaatatt accttgatcc
     5521 cctctcttca aatccggttt gatccttcat cggattcaga atcgatcgaa cagattgaaa
     5581 atcagcggat tcccagactt tatcgaacag atcttccgga tccgaatcgc caacaaaatg
     5641 atgcagcggc acctgctttc tatacgttga atatttcggt taacggtata cctgtagaaa
     5701 agtcacccgc ctccgatgaa accatcctcg ttcaatggag tttgtttgat caattggcag
     5761 ccgagaatga tttgccttcc tgtttaatcc cattgggaga cggagactac caaatgacat
     5821 tccattccga ttggactgcg ggttgttctt tccgaattcc catggatttc cccataaatt
     5881 ctgcaatctc tcctttgaga attcaatttt tatcgcctct ttatccgacg gagactacta
     5941 taccggtagc tccggaacct ttcacggttg agacagccaa agcttttata accgttccgg
     6001 ataaaatcct tctttcggaa aatcagcaaa tcgcggcaga tttaaatcct gtttctgccc
     6061 ctctttcccc ttataccgcc gcattgatca gacggttgcg gggagatctt ttggggattc
     6121 aatgggaata caaacagaat ttggattgcg gcgacgcttt gaatcaaagc aaggattttc
     6181 tatatacctg taatttcaat tttgatcggc tgccatcggc aaactttgaa tcaacggttc
     6241 aactggaggt gagaccgacg ggaatcgaaa aattgctgga aatccaattg ctgtccgcag
     6301 ccagcggtca accgcagaat tctttcccat ttttaccgat cgaaaaaatc cccgccgaac
     6361 tgacagtcgg tcccatttat tcgcccgaat ctcccgatat tcctttaaca tcggtccccg
     6421 tcgggacaaa tgcacagatc gatatcggtt tcaacgaaaa ttcacagcca ttttctatcg
     6481 aaaatctgat cgtgacgctc gaaggcactc ccttggagac ttgttcgatg aacgggaaca
     6541 attccgttcg ttgtacattg ccgacgagct gttctgatct ctcggaaaat caaacctgtg
     6601 aacgagattt aacgatcact gcctgttatc aaggggatga aatccatgga agcagccaac
     6661 cggtcagtat ttcttatcga atcgtccgtt atccgatcaa tcttgaacct tcgaagaata
     6721 ccgtttttca atccgacctc ttgcagcata ttgagcaata taatgaagag aatgggaatt
     6781 ggcttccatc atcgattacg attggaaata ccacatttga ttcgtatttg gttcgggaga
     6841 ccctttcact ttccgggcag tccgagccgg ccgcttatcc gttttttgtc aattactcat
     6901 taaacgggtt tcaatccgct caacctcttc cggaaaattt ttggttggat ctgaccgtcg
     6961 gaaaatcttc cggcggagga atcacggaag aaatcatttc tattccgccg acatcggtca
     7021 atccgaatga atccgaaatt ttattctcac tcgatttcgg ctcgaacctt cagaacacga
     7081 acggttatca accgggtcga caattcgatg aggtatttga aattcgtaaa atcgaagttc
     7141 gatatgccgg agatactttg atcgctccgg tctccgtttc ctatcaaccg gaaggattcg
     7201 agttcccgat caaattgatt tcgatgtttg aaatccgact cagtttccaa gccgaatccc
     7261 ttgatttcgg cggaacaaca ccggtccccg gaatcgatca atatactatc ttctgttctc
     7321 agccggcggc tccattaacc tgtgccgccg atccgaccgt tccggaaacg ggctgttggg
     7381 gaaatgttac gctcaatctt tcgatgaatc cgcaagtgtc agccgcaccg gtttttgcac
     7441 cgcgctgtta tttgggagga attcgtatcg ataacggatt cgaccaaatc tggctgggag
     7501 attcactgaa tcaaaccttt tatccgtgag gagggatttc aaccaaccgc ccgtttttaa
     7561 aggattctgt cgttgcgaaa gcaatctgag tggctttggt tccgtcatag acagtgaccg
     7621 ccggtttacg tccttctcgg atacaattta cgaattcctg catttccgaa agatatgctt
     7681 ccgcaaatcg ttccggaaag gcttcaacac attcggtgac tactccttgc tcgcagtata
     7741 gcatcgccag atttttttgc ggaatcgggc tgatgcgaat cgcgcctttg gtcccgacaa
     7801 tttcggtctc gatatgatag ccatgcgctg ccgttcggcc gctgtgaatg gttcccatcg
     7861 ccccgttttc gaaccggaac aaggcgcaac ccgcttccgc atcccccaac gcagcaaatt
     7921 gcggaaaacc atagctgcct ccgatggcat atacgctttt gggttctgca cccaaatacc
     7981 agcgcattaa atcgatatca tggatagcca tatcgatgaa taagccgccg ctcgtcccgg
     8041 caaaacggag ggaaccttcg ataaatttca gaggatcaat accggtggct ttgaccaagt
     8101 agggttcgcc gattgtaccg gattcgattt tttgttttgc ataaatataa gaaggatcaa
     8161 agcgtctcat aaatcccaac ataaagacct tatcgggatg attttcaacc gctttttccg
     8221 ccaaatagca ttcctcaacg gaaattccca acggtttttc acagaaaacg tgcttaccgg
     8281 cattcaacgc cgccgttaat tggttgcaat gttcgcctga ggtggttacg atggcaacgg
     8341 catccatatc ggcatttttc aacatctccc gaaaatttgt ataacagtcc ctgacgccga
     8401 gttcggtacg ggcatattcc aactcgcttt ccaacaggct gcaagcggct gtcaattcgc
     8461 aaccggaaat ccgatcagcc aagttcttgg cgtgaatttt gcctaatcgc cccaagcccg
     8521 caataccgat ctttaacttt tccataaggt tctccttcgt tgtctcaaac ttttttttat
     8581 cgaatgtgat tccaaagcca aacggttatt tgttgaaaaa tattgaaagt gttcagtcct
     8641 ttattgcgat tataactttc agcccaaagt cagaaagtga aaggaactct ttttcatcat
     8701 aaaaccaaag aattaagaaa tttgtcacgt cgtatatcat attaaatatt atttttataa
     8761 cctattagtt tttctgttta tcttgatata atcataaaat gtaaacgttt acgttaaccc
     8821 atatttgtgt tttcagctta gaattgaatt tacttttagc cgttctcaac aatttagttt
     8881 gaaccatagg agggaaaaat gaagaaattt gtattgatgt tcctgattgt tattgcagca
     8941 acaggtgtgc tcgtcagcgc agtcggtgca gaagaaacct ataaaatcgg ttatgcctgc
     9001 aacaatttta atgacacgtt tcagacagtc attgaaggtt atgcaaaagc ctatgcaaag
     9061 gaaaacggtg ttgaatacgt ttattccgat gccggagaag atattttaaa acagcaagac
     9121 caaattaaag ctttcattgc cgaaggtgtc gatgctttaa ttgtggtgcc ggtcgatacc
     9181 agcgctgccg aaccgatcat tgatatggcc accgaagcaa aaattccgct ggttttcgtc
     9241 aatcgcaatc cttttgccgg cgtgaaaccc gaagatattc cggaaggagt ctattatgtg
     9301 ggttcggaat ccatcgatgc cggaattttc caggctgaat atttggtaaa actgctggga
     9361 accgaaaagc cggtcggtta tgccatcctc gtcggaatgc tctccaacga agccgcagta
     9421 gcccgaaccg aagggaataa acaggtttta agcaaatatc cgaattacaa attattggct
     9481 gaaaagaacg gtgactggca acgtgatcaa gggttgacca tcaccgaaaa ctggctcact
     9541 gcctttggcg atgaattaaa tgcaatcctt tcaaataatg acgagatggc attaggtgcg
     9601 ctggaagcgt tggaagcgaa cggccgggat gatgttatcg tgatgggtgt cgatttaatc
     9661 gacgatgcaa agaccgccat cttggaagga cgtctcgatg cttctgtgct tcaagacggt
     9721 aaaggtcaag gtgaaggcgc gatgaaaatc gctttggctg ctctgaaggg tgagaaagtt
     9781 gacccgatta ccatgatccc gtatgttctg gtgacaccgg aaaacattaa ggattatgtt
     9841 gaataaatcg attcattagc tgtaaacagg gaaaagagcc gtacttttcc ctgtttattt
     9901 ctcaatctgt aaatcagttt gttttaaatt ccaaaaaatg aggggaaaac aatggataat
     9961 gacaattacc tgttacgtat ggtaaacatt gataagtcat tccccggggt acaagcgctg
    10021 aaaagcgtca atttgaacgt cggttatggc gaagttcatg cgctggtggg tgaaaacggc
    10081 gcaggaaaat caactttgat gaaatgcctg atcgggattt attcgccaaa cggaggcgaa
    10141 atctatttcg acggtaaaaa aattgaaaat tacactacag ctcaagcgtt acagatggga
    10201 atttccatga ttcatcagga attatctccg atcctttatc gccctatcat ggaaaaccta
    10261 tggctcggcc gagaaccggt caataaattc ggctttgtcg accataatca aatgtacaaa
    10321 caaagcttgg atgtgctgaa agaaattgag ctggatgaag atccgcggac gttgatggca
    10381 aatctgaccg ttgcgaaaat gcaaatggtg gaaatcgcga aagccatctc ttacaatgcc
    10441 aaattgatca taatggatga accctcttca gctttgacgg agcacgaaga agaacaacta
    10501 tttaagatta ttcgcaagct gaaggaatcc ggcacatccg tcatttacat atcacatcga
    10561 ctgtccgaaa tcatctcaat tgcggataag gtcaccgtga tgcgtgacgg acgggatgtc
    10621 ggaaccgaaa acatcgaaaa catttctctt gaccaaatca taacgatgat gatcggtcgg
    10681 acgatggatg aaatgtatcc gaaattaaaa gtaaatatcg gcgagccgat tctgcaagtc
    10741 aaaaacttat ctcatagaaa gttctttaaa aatgtctctt ttgaagtccg gcgcggcgaa
    10801 atttttggaa ttgcggggtt gatcggcgcc ggtcgaacgg aggttatcga aactattttc
    10861 ggcattcgtc cgaagtccga aggtgaaatt ctcattaatg ggaaacccgt tgaaatcaat
    10921 tcaccgcggg atgcaatagc caaccgcatg gggttcctga cggaagatcg gcgtgctacc
    10981 ggtatctttt ccgtattggg cgtggatgtc aatatggcaa ttcctaatta tgaccgtttt
    11041 gtcacaaaat tcggatttgt aaacgaaaaa gccgtctctg aaagttgtat gaaatttgtc
    11101 aacgaaatcc agataaaaac accgagcctc actcaaaaaa ttcaaaatct cagcggagga
    11161 aatcagcaga aagttttgat ggctcgttgg ctgatgaccg aaccggatat tctgtttctt
    11221 gatgagccca ctcgcggaat tgacgtcggt gcaaaagcgg aaatatacaa gttgattacc
    11281 atgcttgcgg cggaagggaa atgcatcgtc atggtctcat cggaacttcc tgaaatttta
    11341 ggcatgagtg atcgaattat ggtcatgcat gaagggaggg ttacagccat tttggacaat
    11401 ggacccgata ttacgcaaga agtaatcatg cgttatgcaa ccggtacgga aaacgatttt
    11461 aaagaagaat atagaggaat ctatgcaaaa gaataaattt tcttttcaaa acatattcga
    11521 gaaatacgga attgtttttg ttttgattct gatgattgta ttaatgagca tcgcaaaacc
    11581 cagtttccgc accatgaaca atttcctaaa cattctgacc caaagctcaa tctatgggat
    11641 cctcgctctg ggaatgacat tggttatcat ctcgaaaggg attgatcttt cggtcggatc
    11701 gattttagga ttatcggctg tgatcgcagc atctttagga caaagcatga cagcttctaa
    11761 caagtatttc cccgatatgg gagaactccc gattattatc ccggtaatcg cagcgttgtt
    11821 ggtcggcagt ttgtgcggcg caatcaacgg cggtttaatt gctttcaccg gaattcccgc
    11881 tttcatcgct acattaggca tgacgactat tgcgcgagga tttgcctata tttacacgga
    11941 tggaaaaccg atcagtacat taacccccgc tttcaaattt atcggccagg ggaaaatttt
    12001 gggcattcct atgccagcgg ttatatatct gatcatgatt atcatcacct gggtgctctt
    12061 aaataacaca cgattcggga agcgggtata tgctgtcggc ggaaacatca atgcagccga
    12121 agtctcagga gtcaacgtca aaaagatgct cttcctgatt tacacttata tgggattgtt
    12181 ggccggcatt tcggccattg tctcaaccgg aagaatggtt tccgcacaac ccggactggc
    12241 tgccggctat gaattaactg caatcgcttc cactacgatc ggaggaacca gtcattccgg
    12301 cggaatcggc acggtttggg gcgctgtggt cggcgcactg gtgctcgcgg tcatgagaaa
    12361 cggaatgact attttaggca tccattctta ttggcagcaa attgtcgaag gtttggtgat
    12421 tattattgcg gttatccttg atatgcgtaa aaatgccagg aaaaaataga atctcctttt
    12481 tttatttccg aacggccgca ggttttgatt ctgcggccgt tcaattttac ctatcatgat
    12541 tcctttcgaa ttaccccgag gttacgaacc gtctcggtaa cactttcgct atgcaaatca
    12601 tctacattaa aacggagaca cttaaaggta aattgaagga taaagctgat tccgaagaca
    12661 gcgataacgg ttccgaggcc cactttggct cccatcagcc aacccaacaa cagaaccgtt
    12721 ccttcgatca taccgcggat catccctacc ggaataccgc tgaacttctt ttttaatccg
    12781 atcatcagag agtctcgcgg accggcgccc aaagccgaac cgatataaaa ataggacccg
    12841 agacagatta caaataagcc gaacaacatg aaaaggatcc ccaagccata gttatcgatt
    12901 cgcggaataa aattccagtc gataatcaga tcgcaaaaag ttccgataaa aatcatattc
    12961 aatacggttc cgaagccgat tttctctttc aaaataacgg ataccataac aatcaataaa
    13021 ccgaccagaa taacgatttt cccgaatgaa atgccggttt tttgcgataa acccatatga
    13081 aaagcttccc atggagctaa accgacgttg gcttgaatcg taattacagt tcccaatgca
    13141 taaagaaata atcccaggaa tagtctcaat aaacgaaaaa agtaagattg aatgattttt
    13201 ttcaattttt atacagttac atttcacccg attttatgac ttgatgatgc tgtcacaacg
    13261 caaaaaacat gggaacattt tcaaatggat gcaatatttt ggatggtatc caaaacgctt
    13321 tcacttttca cttttttcac atcaaagtgc attaaccgaa acgtaaattg cagaataaaa
    13381 ctgattccga agacggacaa aattgtcccc aatcctactt ttgcacccat taaccaaccg
    13441 aaaaaaagga caatgccttc gatactgcca cggattgctc ctaccggaat tttcggaaaa
    13501 cgtttattca atgcgatcat aatcgaatcg cgcgggccgg cgccgaaacc cgcgttgata
    13561 tagaaataca ttccaaagct gttgataaat aatccgatca gcataaaaac aatcccaagc
    13621 caatagcttt cacaagtggg aatgatattc aaagacagaa taaaatcaca gaatatgccg
    13681 ataaaaagta cattggcgac ggtaccgaat ccgaattttt ctttcatcgc gaccgaaaca
    13741 atcagcaaaa taacaccggt caaaataaca acttttccga aagagattcc ggtttggatg
    13801 gaaataccca tatgaaaagc atcccaagga gccagaccga tattcgcttt aatcgtgatg
    13861 actgtaccga ttgaaaaaag aaaaagtccg aggaataatt taataaaacg aatgagatat
    13921 cgacgcattt tctgatcttt tgaattttta ttttaacatt aaaacatccg attgaatgag
    13981 ataccatact tcatatgaga acataaacac cgaagaagcc ggagacctga gacaaaaaga
    14041 aaagcttccg aaaatcagaa gctcttcttt ccttttatcg attcttcaat cagcactcgc
    14101 tgtagccgca actgtaacat ttccggcagc cttcctcgtt gatgacggac gcctgaccgc
    14161 attccggaca aagatcaccg atcctcagca taggattttg cgtcggtgcc attcgtcgcg
    14221 aagaatcttc tcttgccttt tttttcggtt gaacgtctgt accctgctca agtaaacgtt
    14281 cttcccgttc ttccaaatat tccgccagca cttgagccag cccatccggt aacgaacgaa
    14341 cccggtttgc gccaaagcct aaacttcgcc ctccgccgat atcacgcaac tgatcttgaa
    14401 tatcccgtaa tcttcggaca ggttccaatg gggatgcgat tcgcagcgta taagagatta
    14461 atcggccgat cgcttccgaa attgcggaag tttccgagcc ggctttcgcc gtattgataa
    14521 atacttcaaa cggttgattc ccgccattct cgttaatcgt cacaaatgcc ttccccaaag
    14581 gagtttcagc ccggtaggta aatccgcgta atgccctggg acggggtttt tttacttcat
    14641 accagagagg tatttgagcg gaatcttctg actttaacgc agtcggaggt agtatttcag
    14701 acatgccgat ttgctgtttt tcttcggctg tttttttcgt ttcaagcacg actttttctc
    14761 tggaaccggt gacataaaca gtggtccctt tgcagccgag tttccacgcc agcacgtatg
    14821 cctttgcgat ttcttccgga ccggtgcctt cggggaaatt aatcgtcttt gacaaactgt
    14881 tatcgacaaa agcctgaagg gaggcttgca tccgaatatg ttcttcggcg ctaatatcgc
    14941 cggaaactac aaaagttcgc cgaatttctt cgggaatttc ttcgattccc tggcaggatc
    15001 ccttttccat tactttatcg accagtcgct ttcgggtact ctcatccaaa ccgcttttga
    15061 tcatcgcttt ctcaaataag ggactggcat aagataaaag aacatctttt ccgttgtcgt
    15121 tcatatgacg gacataagcc aatgcgaatg ccggttcaca cccgtatcct tcgcatccgg
    15181 taacggtcgc aattgtcccg gtcggggcaa tagttgtttg tgccgcattc cgaataccgt
    15241 tccgatgcat gccctctttg atttgttccc aatccgtctc gggtcgattc caatcatgcc
    15301 gatatggatt cagaggagtc ggaatctgcc atttaatcgc atccggatca taaatcgagc
    15361 ctttgattgc gggaaacgca ccgcgttctt ccgctaatcg aatgctttcc ttcatcgaat
    15421 gataacggat aaattccatc acctgggaag cgaattcctg tccttcttcc gagccgtatc
    15481 gtataccgcc gttgaacatt aaatccgcca aacccatgat cccgaggccg atccgtcgcg
    15541 ccctcaatgc cgcttctttt agttggggga cggccggaac atatgcgttt acgtcaatca
    15601 catcatccaa gaatcgggtg gcaagaacag tgctttgctc taatttttcc caatccacac
    15661 tgccgtcttc caaaacatgt tgtgccagat taatcgagcc cagacaacaa ttttcataag
    15721 gacccaacca ttgctctccg catggattcg tggattccaa tcgataaagg ttgggaaccg
    15781 gattaaatcg attcgcctga tctataaaca atacccccgg ttcaccgtta tgatgcgctt
    15841 gattcacaat caaatcgaat aaatcccgcg ctcgcaccgt cttatacacc ttaatcggca
    15901 aaccctgatc tttggcatcc tgcacatctc catcgaactc cttggtcaga ggagaagtta
    15961 aatcgggata aatcagatcc caagaggcat cctcttcgac cgctttcata aaatcatcag
    16021 ttattccgac ggaaatattg aaattggtaa tcgaattttc attggtctta caacagataa
    16081 aatcttcgat atccggatgg tcgattcgca aaaccgccat atttgcaccg cgccgcgatc
    16141 cgccttgggc tatttcgccg aaagcgtgat catatacccg caagaaacca accggtccgg
    16201 tggcctgtcc cgctgaggaa cggacaatcg ttcctttggg gcgtaatctg gaaaaagaaa
    16261 aaccgtttcc cccgccggtc tgttgaatta atgcggcatc cctcaatgtt tggaaaatac
    16321 ccgttgattt tttccccata tcatcgctga tcggtaaaac aaaacaggca gccagctgtc
    16381 cgagaggtgt tccggcgccc gtaaatgtcg gagaattggg aaaaaataac ttattaacca
    16441 tcaatttata gtaagcaatc gcggtctcga tcacatcacc gccataattc ttttcggcaa
    16501 gagccacatg ataagctacc cgccaaaaca tctcatcgat agtttcaatg ggtttcccgt
    16561 cctcgccgcg tcggacataa cgcttggtta acacttggat ggaattttcc gtcagagata
    16621 tggaaggtaa tccttccggc atcgggggtg tgtaaatttt atcaggttga tgatcttttt
    16681 cttttgcgtg ttccattcaa ttctctcctg tcaactttct gaaagcagac ggtcaatttc
    16741 ttttcgaagg gataatagat catccaattg caaataaaca atcgcatagc ggacataagc
    16801 aatctgatca agtgatttta aaccggcaat caccttgtca ccgattaccc gggaagatat
    16861 ttccctttgt ccttccattt gcaattccct ttcaatatca tcgactaact gctcaatttt
    16921 ttctgccgga accggccgct ttgcacaggc aatccgaatc ccgcgcagca gtttttcccg
    16981 gctgaattcc tgcctggaat tgtccgcttt tacaaccatc ggtatcccaa gcagcggtcg
    17041 ttcgtaagta ttgaaacgct ttccgcatgc cggacattga cgccggcgat gaaccgtatt
    17101 tccttcatct ttatcggtat cgattactct ggttttgggt tctccgcaaa aagggcaatt
    17161 cataggtcag ataattcaat tctccgaata aaacaaaaaa aagagggtta tttttattgt
    17221 aagaatacct gctttccgaa ataaaaataa ccttagaata tttttgacga ttgttaatcg
    17281 cttaataaat cactatatat ggtattttat ctatccgaaa tccctttata tagtgtaggt
    17341 aattttagca tgaaatatgc attcaaacaa gggagcagct gaaaacttta aggttaaaat
    17401 ttttccgact gtttattatg gggcgataaa ggtcttatac cgacataatt tcatcgtttt
    17461 aagacaaaaa tgtctttcaa atttacctct tttatttgaa aagtatcgga tcattaaaga
    17521 ataagtgccc ccagagaaat tcgaatcctc gttttggcct tgagagggcc acgtcctggg
    17581 ccgctagacg atgggggcaa ttgaacggtt tttattttat catgaatcac ggaaaacaat
    17641 aaaaatgaga ggttatatcg aaatccaatc gagaatttta aaaatcaaac cttgctgtaa
    17701 ggcaatattt ccaccgtcga catacatttt caggaaccat gttcccgtaa acaaaataaa
    17761 gaaggcgatc cattgaacgg tttgatagcc ggataaaacg gtgcttcccg cgaccgaatc
    17821 ttcacaagaa aagtgctctt tgagcacaac gagactcaga tagatattaa aaccatacaa
    17881 ataagctaat cgcccccaat cggaactgaa tataaaaatc gtcatggtgg aaagcagcag
    17941 aatcacggag aaaatgagta accccttacg gtcttccaaa aattccgata ccttattcag
    18001 gatgtgatag cgagcattca ataataccat cgggatcgca gaaagagcca taccggcaag
    18061 gtaagagaaa aaggtttgcg gataactcaa tttgtcaaag acaggctgta agccctcctt
    18121 caagggtact cccaaccaac cgaacgcacc ggtttgaatc gtcagatggt agtcttcgat
    18181 taatccttcc caagattgcg gaatcaagtc caggttgtaa taacgtctgg ataaagcata
    18241 aagccaaccc agatttccca aaagaaacgc caatgaaaga atcgaaaata gatataagtc
    18301 ccgtttcgga cgattattat aaacaatgaa tatcaatata acgacaaacg gaaaataaaa
    18361 taaagcatat tcatgaatta atactccgat ttcgtagagt atcagagttg acggaactaa
    18421 aatcgaccat ttcactttct ttaagaccat caaaaaaaca atcataaaaa tcaagaccag
    18481 gaaaacatct ttccgtccgt aagcatcgaa attgtagatt gggaataaaa aagccaccgg
    18541 ggagaaagta aataaaagcc aatcctgaaa atcgatctta cgggtaattc gcagatacac
    18601 gatcaaatga agcagatagc aaattgtgat cagagccgaa agaaaaaatt tcacggatat
    18661 atagggagaa acaaggtaac cgattcgtcc gatcaatcct ctgcggataa agccggcggc
    18721 atagttgatc aggagttccg tccaagtata ggtatccaat ggagatcgat aaagtttcag
    18781 tccggaggaa aggataagaa ccgctaaaat taccagagaa ataattcgct tatttttttg
    18841 atccattttt aaccgccccc attttcagga cctgtttgtg aatttgactg gttttattat
    18901 aactgttctg attttcagcg catcgtttct caaacatgac accgttttat cgtcatattt
    18961 attcgtcacc cattcgctct gttaaaaaaa ttcatttttt tcgattaaaa tgattgagaa
    19021 aaatggcaaa aaacatgtat gacttttata aagatgctta ttccgagatt aaggggaaga
    19081 tgcccgaacg cccatatctc gacaaacaga tgtcattacc gatttatgaa ggatattccg
    19141 tcgccaattt accggcaagc atttgcaatt ggttaggatc tgaaaacacc ggaatgctcc
    19201 cgcctttagc tttcgaaaaa atctacggtg atcgctttcg gcatgtcatc ctggtcatag
    19261 tcgacggtct gggcgcttat cagatgggaa tgatccggga acgtttggca gataacttat
    19321 ggctgcggtt gatgtcggaa tatggaacgg aaagactttt aaccagtatt gtcccgagtg
    19381 ccaccgccgc agcgttaacc tctctttgga caggcgtttc aggcggcact catggattgg
    19441 tcggatatga gacttggatg cgcagcctcg gaatggtcgt caattttctc caatactctc
    19501 cttcgtcgat cggctccaaa cccggattaa tcggggatgc cggtattctt ccggaagcca
    19561 ttcttcccgt tccgacattg ggaaatcatc tgatgggagg gggaatcatc agccgctctc
    19621 atatgccgat tgccctcgcc cattccaatc tgactcgggc acaaatggcc ggagtttccg
    19681 tcgtcccata caggcgattc ggtgatttat gggaaaatgt caaagaactg ttggaagcgg
    19741 atcgcgaaaa agtaactttt gactccgttt attggaatga cctggatacg tataatcatt
    19801 tgatggggat gaacgataat cgaattttca tcgacctggg atcctttttc gatggtctgg
    19861 cgagaacggt tcaagctctg tcccgaggaa acttcggaaa gacattggtt ctggtcaccg
    19921 cagaccacgg ccatatcgaa aatacaccgg atcctaaccg ggatatcaaa aatcatccgg
    19981 aattgatgca taatttgatg attatgcctt gtggagaaaa ccggctgacc tatttttatc
    20041 cgaaatccgg caaagatacg gcgattcgta attatatcga agaaacttgg ccggatgact
    20101 tctacttggt cagcgggcaa gagctgattg cggacgggat attcggaacc gctgctttcc
    20161 atcccgatct ggataatcgg atcggtgaat tggtcgcaat agcccgcgga tacgcctatt
    20221 tttggtggcc gaatcgtccg gataaactct attcgagaca cggcggtctt tcggatcagg
    20281 agatgatcgt cccgctgatg ggtttggcgc tctaaatgaa ttcaagtctt taaaggatgt
    20341 gaaaacttat gacaattgac aggattgctg ttatcggaca aggatatgtg gggcttcctt
    20401 tggcactcag ttttgctctt cacgggtgtc atgtcattgg cgtcgatagc gaccctgatg
    20461 tttgttccaa tctgaaaaaa ggaatcaccg cacaaacgga aacctatcag ggccaatcga
    20521 ttcaaactat tcttcagacc atgctgcaaa aaggaacata tcagatcacc gacaacggag
    20581 ccgaggctgt caggcgatcg gaggtaatcg ttttaacggt cgggataccg atcgaatcgg
    20641 gtgttcctgt ttacgagtac tttgaagccg cctgcaacac gatcggagaa aaccttcaaa
    20701 aaggcagtct gatccttatt cgcagcacag ttattcccgg taccaccgag aatttttgca
    20761 agccgatatt tgagaaaaaa tccggaatga aagccggggt tgatttcttt tttgcttatg
    20821 ttcccgaacg aattgcggaa gggaaagctt ttgaagaatt tgagacgatg ccgactctga
    20881 tcggcgctcc ggatgaaaaa agttttgata aagctgcagc aattatccgc atgatcagta
    20941 aagccgatat catccgctcc gattccgtca tggcggtaga aacatcaaaa gtactggaaa
    21001 atctgcagag ggacgcaaat atagccatag ttcaggaatt tgcccgattt gccgaagcgg
    21061 ccggaatgga tatcatggaa atcatccgat tagccaatac acacaagcgg gtcaatttgt
    21121 tgactcccgg acccggggta ggcggttact gcattccgaa tgccttccac tatctcaacg
    21181 tcaaagccga tgaattggga atcgaattgc ctttgttgaa attagcgaga cagcaaaatg
    21241 atcgcttacc gcaatttttc gcggataaaa ccgaagaact cttgcaagct gccggaaaat
    21301 cgatcgctgg ggcaaaaatc gcggtagcgg gactcgccat gaaagatttc tcaccggacg
    21361 atcggctgag tccggctgta atgatctgca atttactgat ccgatccgga gcaaaggtag
    21421 ccgcttatga caaaaacgtc gccactcgcc atccttttaa agtcgattcc tttagagaag
    21481 ctgcgaattc cgcggatgcg cttctcattc tcaacaggat ctcgagagat tttaatccgc
    21541 ttgaatggat cgatgagatg aaaaaaccga tagttatctt ggataccaga ggaataatcg
    21601 aacccgaaga tctgcccgag gggaccgttt tctggagaat ttgattcgaa ttcccttttt
    21661 aaatatttgt taacctttca ttagaaaacc tcataaatgc ttgacggtca ttttttggcg
    21721 tggtattata ctgtaggttt gattagcact ctaatcgcaa gagtgctaac ggggcgaaaa
    21781 tggaaacttt gacggaaagg caaaaattaa ttctcagttt ggtagtccat gactacacaa
    21841 aaaccgcgag acccgtgggt tctaagagac ttgtcgaaaa attcaggtta gatttcagcc
    21901 ccgccacagt ccgaaacgaa ttggctgctc tgacggaaat gaacttttta cgtcaaccgc
    21961 acacctcagc cggaagagtg ccaaccgaaa aagggtaccg atattttgtc ggaacactga
    22021 tgcaaacaca ggaattatcc gaagagaatc gaaggatgat cgaacatcag tttttccaaa
    22081 cccagcagga acagggaata gacggatgga tgaaactggc ggcatcgatt ctcgcggtgc
    22141 agtccaatgc gatttcggtc gtctctgccc cgatgccggt tcggaacgga ttgaagcacg
    22201 ttgccgtgat cggaatcaca gggaagcaga tcctgttagt gctcgttttg gacggcggcc
    22261 aaatgcaaca gcgattcata acgcttgatg agcaaacatc gcaaaatgaa ttgactgtga
    22321 ttgcgaatga aattaatcag cgatttaaag gcatgactac cgagcaaatt cgcaaggaaa
    22381 gtcaaattga gaagtcaaga gaccctttaa tggaactgat tttgaaaaac gtattaaatg
    22441 cgatgtacga aacagatgca atgaaagccg gagaagtcta taccgacggt atcagtaacg
    22501 tcttagccga accggaattt tcggaatcgg aagatgcgcg ccgggcgttg aaaattctgg
    22561 aagaaagacc tgccctgcag aactttcttt caagaacgac atgggatcag aagatcggcg
    22621 gtgttcaggt actgatcggc ggggaagggt tgttggcaaa tttgagaaac tgttcgatgg
    22681 ttttagcgag atacggtcta ccggacacat tgaccggaac ggtaggtgtg atcggaccaa
    22741 tgcgtatgcc atacagccga aacatcccga cagttcgatt tattgccgga ttgctcagcg
    22801 atcttgtttc ggaatcgctc ggataaaaaa aacctattca agcggataga ataaaaaaat
    22861 acagggaata gatatggcga aaaagagtag agatcaaaaa gataacagag aaattccgga
    22921 aaaagaagag cgtgctgaaa atactgaaat caacgatgaa aacgttggag ttgacaaaaa
    22981 ccctgatttt tcagcacccg acggctgcga agaagtgctc gtcctttcta aagccgaagt
    23041 ggatgagatg aaggaaaagc ttcatcgttc tcagcaaaag gaaaaggaga atctggagag
    23101 ttggcaaaga gagcgcgcag attttataaa ttataaaaaa aggattgacc gtgagcagga
    23161 gatcttacag caaaattata aagctgaact gctgaaaaaa tacttggtca tcttagatga
    23221 tttggaatta gctatgcgaa ataagcccga agagggagag gatccgacca attggatccg
    23281 agggatcgaa ctgattttgc gaaaattcga atcgatcaat caaaacgaag gattgaaaaa
    23341 aatcgaggcg gaagggcaag aattcgatcc aagatatcat caggcaattt cgcacgaaga
    23401 agacccgaat gttgaatcgg gaaaaattat tgaagtatta caaaacggat atatgttagg
    23461 agaccgcgtc atcagacccg ctctggtacg cgtagcgaaa taggagaata aaatatggga
    23521 aaaattatag gtattgactt aggaactaca aactcagtta tggctgtcat ggtcggtggc
    23581 gagccgacag taattccgac cgcagaggga gaacgaattt gcccgtcggt tgttgctgtc
    23641 aataagaatc atgaacgatt ggtgggacgt gttgcccgta accaggcaat aaccaatccg
    23701 gaaaacacga ttttctcaat caaacggttc atgggacgaa aatacgacga tccggaagtc
    23761 caaaagacat taagccgaat tccgtacaaa gttacaaaag cacccaacgg agatgtgaga
    23821 gtggtgttgg acgggaaaga atattcggca ccggaaattt cagccatgat tttggcaaaa
    23881 atgaaagctg acgccgaagc atatttgggc gaaaaaatta ctcaggcggt tattacggtt
    23941 cccgcttatt ttaatgacag tcagcggaat gcgaccaaag acgccggaaa aatcgccggc
    24001 ttggaagtgc tgagaatcat taatgaaccg actgcttctt cactggctta cggcttggat
    24061 aagaagacca atgaattgat tgcggtttat gacttgggtg gcggtacatt cgatatctcc
    24121 atcctggatg tgggggacgg tgtattccag gtacggtcta catcgggtga taccttcttg
    24181 ggcggcgatg acttcgatca gcgtattatg gattatctga tcgatgaatt taagaaagca
    24241 aacaacatgg acttgcgcca ggatcggcaa gcgttgcaac ggttgaagga agcggctgag
    24301 aaggcaaaaa tcgaactctc atcgacgatg cagacggaaa tcaacttacc gtatattaca
    24361 gccgatgcca ccggtccgaa gcacttggtg atgaccttga cgcgtgcaaa ggttgagcaa
    24421 ttaacttccg atctgatcgc acggagtttg gaacctgtcc gcaatgcatt acgggatgcg
    24481 aatctgaaga ccagcgatat caatgaagtc gttttggttg gcggtatgac ccgtatgccg
    24541 gcagtccagg aagcggtaaa acgtgaattc ggtaaggaac cgaacaagag cgttaacccg
    24601 gatgaagtgg tggcggtcgg tgcggccatt caagccggag tcctcggcgg agaagtgaac
    24661 gatattctgt tattggacgt gacacctctg accctgtcgg tcgaaacaat gggtggagtt
    24721 gccacaccga tgatcgagcg gaatactacc attccagcga aaaagactca gatctactcg
    24781 actgcagcag actcacaaac ttccgtcgaa attcacgtcc tgcaaggcga acgaccgatg
    24841 gcagccgaca ataagagcct cgggaaattc atcttggacg gaattccgcc ggctcgacgc
    24901 ggcgttccgc aaattgaagt cacctttgac atcgatgcaa acggtatttt gaaagtgacg
    24961 gcggtggata aggctaccaa ccgcagccag aatattacga tcagcgcatc atccggtctg
    25021 accgatgccg aagtggagcg aatgcgtaag gaagccgaga tccatgcgga agaagaccga
    25081 aaacgcaagg aaggaatcga gatcaaaaac aatgcggata acgccgtcta tacagccgaa
    25141 aagttgctgg aggaaaacgg ggataaaatc ccggccgata tgaagtccaa catccaatcg
    25201 aaagccggtg aattgaagaa tatcctcaac ggaacggatt tcgatacgat gaaacggatg
    25261 acggatgaat tgaacaagat gattcaagac atcggaaaca tcatgtatca gcaacagcaa
    25321 cccggtgccg gacagtctca atcggatcaa ggcagcggtg catcaggcca aaatcctccg
    25381 acagggaacg acgggaatga tgatgttgtt gacggtgaat tccgcagcat gtaatcacct
    25441 agttcggtaa aaagaataat cgctgtaccg cgaaccttta taataataag gatacggtac
    25501 agcgatttgt tgttccgtca tccccggttt ttaccgggaa gtttttatga tcgagaagtg
    25561 gtaaattaat ggtaaaagat cgagattatt atgaagtctt gggagtcggc agaaatgcca
    25621 gctccgatga attaaaaaca gctttccgaa acctcgcaag aaaatatcat ccggacatta
    25681 acaaagaccc cggggcagag gaaacattca aagagatcaa cgaagcttac caggtccttt
    25741 ccgacccgga taaacgagca gcatacgatc gatatgggaa agccggcgtc agcggcgtag
    25801 ggaacggatt cgattattct caggttgatc tctccgatat cctcggtgat ctcttcggat
    25861 tcggaggatt tggaggcttc ggaggcttcg gttccacaca atattcatcg cgccaacgca
    25921 atgccccgcg aaggggggcg gatctgcaaa cgaccgtcac tttgaaattt gaagaagcat
    25981 gcttcggagt cgataaagaa gttcaatttt cccgcgatga aaagtgttcg cgctgtcacg
    26041 gaacgggagc cgaaccgggg acttcgcccc aacgatgtta cacctgtaac ggtaccggtg
    26101 aaattaaaac aaatcgtcaa accatgttcg gttctatggt gcaggtttca acctgcccca
    26161 cctgtaacgg acggggagag atcatcagca ccccctgcac gcaatgccac ggacgcggtc
    26221 tcgaacggaa aaccgtaaaa aaggtggtat ccatccccgc cggagtggat acaggcacac
    26281 gcattcgatt gtcgggggaa ggccaaccgg gtgataacaa cggccccagc ggagatcttt
    26341 acgtggacat ccgcgtcaac cctcataagt atttccatcg gaaagatgcg gatattcaac
    26401 tggatatgaa catcaatatc gctcaagcga ctctcggtga tgagatcgat gttcctacta
    26461 tcgacggcac ggaaaaactg aaaattcccg caggaactca acccggaaga gtatttaccc
    26521 taaaaggaaa aggaattcct cgtttacagg gcagcggaag aggagatcaa aaagtggtca
    26581 ttaacgttga aataccgaat tctctgacac cggaacaacg ggatttgatg gaacagttag
    26641 ccggaaccat ggggacggaa gtcattcctc aggaaaaaag tttttgggat cgtctcaagg
    26701 atgcgttaaa tggctgatcc cgcctggctc gaaatcagtg ttgtcgtaga cagtgagacg
    26761 gcggaagccg ttgccgaagt gttatcccgc tacgccgaaa acggcgttgt cgtggaacag
    26821 ggcgtagatt ataacgatgc ggaagatgtg ggaacgccgt tcggtccttg tcgggtaacc
    26881 ggttaccttc cggtggatga aacgacggaa gaaaaacggc agcggattct ggaaggactt
    26941 tggtatttag ggcgcatcac cgccgttccg gagccggaat ttaagtttat tgaagacgaa
    27001 gactggatgg cagcatggaa aaaattctac catccgattc tgatcggcga aaagcttttg
    27061 atccttcccg cttggatgcc atccattgag cccgaacgca tcgcgataaa gattgatccc
    27121 agcatggctt tcggaaccgg cactcatccc actactcagt tatgcctggc gatggtagaa
    27181 aagtacatcg ttcccaatga ggacatcatt gatatggggt gcggttcagg aatactctcc
    27241 atcggagcgt tactactcgg cgcccgtcac gctttgggag tggatatcga taacgagtcc
    27301 atgcaaagca gccgtgagaa cgccgagaat aacggagtgg cggacaggct ggaacttttc
    27361 aaaggtactg tccaggaaat tttgaacgga gcatgttcca tcaggcaagc tcctctggtt
    27421 acagccaata tcctgacgcc catcctcatt caattatttg accaaggaat ggcggacctg
    27481 atcactcccg gtggggtgat tctgctcagc ggtattctcg aagaacagga tgagaaaatt
    27541 cggaatgcag ccgaagcgca ggggcttgaa tttcttgaga agcgccagat tgcagactgg
    27601 gtcgcatatg cgtatcgcaa accgcaaaaa taaatcattt aaaatatccc tttcgcagaa
    27661 attttatttc tgccggaatg aaaataaatt cggatacgat ttatcgattc tttccgaata
    27721 aagatttcgt aagaagtctg attcttcaca aaaaaatgga ctcgaaaact cagcctcaga
    27781 aatgaggatt tgacgttaaa atacttttca tatagataac actacccgtc agaggaaccg
    27841 ctgtatagaa ggagttcctt tagtcagaat agcagacgga aagaataaat cgaaaagggt
    27901 gaaccgacta tgataaattc catacgcgaa cgaatcaagc aattggaaca tcagcgatcg
    27961 atgtccggat tggaaatttt gaaatacatc ggtcccggtc ttttggtaac ggttggattt
    28021 atcgacccgg gaaattgggc ttccaatgtc gcagccggat ccgattacgg ctataagttg
    28081 ctttggatgg ttaccctttc cacttttatg ctgatcattt tgcaacataa tgcggcacat
    28141 ttggggatcg cgaccggtta ttgcctttcg gaagcttcct ccatatacgt caaaccttgg
    28201 ctcagtcgat ttctcctgac cacggcattg ttggcgacag ccgctaccgc gatggcagaa
    28261 atattgggcg gagccattgc gctccaaatg ctttttaact tgccattgaa aatcggcgcg
    28321 attctggtta cggtgctttc tctcacctta ttactgacaa actcttataa attgctggaa
    28381 cgttggatca tcggttttgt ttccctgatc ggtctttctt ttattatcga gattatcttg
    28441 gtagatgttg attgggggca ggcagctgtc agttgggtga aaccatcgat tcccgccgga
    28501 tctatacccg tcataatgag tgtgctgggt gcggtagtca tgccccataa tctttatctt
    28561 cattccgaaa ttatccaaag tcgccaatgg aatctaaagg atgatgaggt tatcagacac
    28621 caactgaact atgaattctt ggatacgatt gtttcgatgc tgatcggttg ggcgatcaac
    28681 agcgctatga ttctggtcgc tgctgcaacc ttctttgcca acagcatcca tgtcagtgca
    28741 ttggacacgg caggcgctat gctcaagccg ttgttgggga acggtgcttc ggttatcttc
    28801 gcaatcgcac tactgttggc gggcatttca tcctcgataa ccgccggaat ggcaggcgga
    28861 acgatcttcg ccggaatgtt taaagaacat tatgatattc acgatattca ttcccgaggt
    28921 ggagtcatcg ccatctttct gttagcgctg gtcgtgattt tcttcatccg agatccgttc
    28981 atggggttgg tttattcgca aatgttgctc agcattcagt taccgtggac gattttttta
    29041 cagatttatt taaccagctc ccaaaaagtg atgggaaagt tcgccaattc caaggggaat
    29101 ttcatccttt tgatgatcat cgggttgatc gtaaccggtc ttaatatcgt attgctaatc
    29161 agttttcttc gataattctt ttgaaattct ctttgacggt ttttcgagat aacgattcgg
    29221 ttgattttcc aagcgcaacc ttgggggatt cttttcgatc cggattattc ctttacggct
    29281 tcgatcagaa aagacgcgac ataatcttcc acattgcttc cgggtaccca ggattttaaa
    29341 atctccttgc tgttatcttt aggggttaag cgaatatttt taaaaccgac tcgttgtaac
    29401 agatctcgaa tcgtatctgc ttgttcggca cctgcgatgc agcccgttaa cagatccgga
    29461 tccttcttta tttcctccgg cagctcggct gtggcaacaa tatccgaaat agataatctc
    29521 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntctttacc ggccatcttt
    29641 tcggcaagaa aacaatcaaa tccgccgccg ctaccgagat ccaatacaat ttccccttct
    29701 cggatggcgg ctatagccag gggatttccg catccgagac ccatattggc ttccgccgga
    29761 gccgataata aatcttcttt ggaataacca tttcgcaaag ccgcttcggt tatgccaaca
    29821 gtaacagaag aaccgcaaca tccgctgcct gcgcagcaac cggtaggaag gtctcggttc
    29881 gcgatcgccg aatacttttc gatgattatt ttttgtgttt tatttttatt ctgttccatc
    29941 tgtcaataat cctttacgaa tgttcaaaag tccgcaatgt gcctaatatg atttttcgga
    30001 agctttcttt gaacttattt ctaaagccgg gaaccaatgt cgtgtattgt ttgcaatgtt
    30061 aaccaagatc aacatcgtag gcacttcggt caatacaccg actgtcgtag ccaaggcagc
    30121 gggagaatcc attccaaaca aagcgatggc aactgcaacc gccagttcga agaagtttga
    30181 agcaccgatc attccggccg gtgcggcgat cgaatagggt aattttgccc aacgacaagc
    30241 gaaataagca ataaagaaaa tcaaaaaagt ttgaataatc aagggaattg cgatcagcac
    30301 aatatgcaaa ggattcctca aaatcgtttg tccttggaaa gaaaataata ggattaaagt
    30361 taagagcaat ccgattatcg ttacctgatc aaatttatgg agaaaaatat tttcaaagta
    30421 atcgacgccc ttgtttttga taaccgtcac tctggtcaga aaaccacccg caagcggaat
    30481 caccacaaac aataaaaccg ataggaccaa agtgtcccat ggaacggtta cattgctaat
    30541 tcccagcaag aattttacga taggaacaaa tgcaaccaaa atgatcaaat catttgtcgc
    30601 cacttgtata acggtataag ccggatctcc cttggttaag gtactccaaa caaaaaccat
    30661 agccgtacaa ggcgcggcgc caagcaatac catgcccgct aaatactgtt tcccgagatc
    30721 cgcaggaata atgtttttaa acacaacgta aaggaaaaaa gatccgatcg caaacatcgt
    30781 aaaaggttta atgaaccagt tggtaatcca ggtaatatat aaaccttttg gattcttacc
    30841 gacattttta atgctttgaa aatctacttt caacatcatc ggataaatca tcacccatat
    30901 cagaatcgct gtcggtagcg aaatgtttgc atattcgaac ctgccgaaga atgccggtat
    30961 ccccggaaga aattttccga tcaaaatgcc ggcaaccata cataaaataa cccaaatcga
    31021 taaatatttt tggaaaaatc cgattccgat attttccgac tttgtattca taagtaaaaa
    31081 tccttctttt ttatttggaa ttctgttgat attttttgca aatacaatcg tctttttctt
    31141 tcataagacg gctgaggaag tcaataagcg tattggactt ctcttgatta atcgtatatt
    31201 tcatccatgc tccgtccctc ttcgcaaata ccaaaccgct gtcggataaa atcttcatat
    31261 gataactgag tgtcggctgg gaaatattga attccgttaa aatatcacaa gcacacatct
    31321 ccccacagga caacatatcg acaattttta agcgagtttc atccgataaa gccttaaaaa
    31381 tcggggcata ctccctatag acatcatcca tgaattacct tcctcatcat atagaatcac
    31441 ttctatcata tagatattta tctatatgat agaagtgatt ctatcatatc ggaaaaacgt
    31501 tgtaaatcgg aagatcttag aaagtgattt ttaccttaga atcaaaaaaa tctgcaaaac
    31561 aaaatcggca aaaacggagc aagcaacaaa ttggccgttt ttatattcgt gagtttcagc
    31621 atattgaatg cgatggctat aatcaataac gaccccgtac aggtgatttc attaatcaca
    31681 ttcatcgtca gatatcccga aatcgcaccc gccgacacag tcaatatacc ttgataaata
    31741 aataacggaa cggcggcaaa agcaacgccg atccccatcg tggaagccat aatcatcgcg
    31801 ataataccgt cgatcaaccc tttggtgaac agcgtttcat tatttccggt taaaccgctt
    31861 tgtaatgaac cgacgattgc catggcaccg acgcagacaa aaagactgca ggtaataaat
    31921 ccttccgaaa taggattatc actttttact ttggattgca aaaaatcccc aaaggattga
    31981 agtttcccgt cgagattgaa taactcaccg ataacggctc cgaacacaac ggacaatacc
    32041 acgacaatcg tgttatttcc ttccaacgat ccgctgattc ccacatacag cacacaaagg
    32101 cttaagcctt gcatcatcaa ctgattcatt ttctcgggaa tacctttatg aaggacactt
    32161 cccaatattc cgccggctgc aacggctgcc gcatttacga taaccccgat taacatagac
    32221 agattcaacc tcgatagaac agaaaatgct tcagatcatt cccgcgatca gcgggatcgt
    32281 caaaaccgac aggagcgttg tgataataat agcgctcgag caaatatcac tgtttacacc
    32341 gtatcgattg gcaagaatta ccggaagatt tccggtgggc atggcaaaca tgattgcgct
    32401 gatcccgata acctgctcag gcagcgggaa cagtttcaac agaaaaaccc cgattgccgg
    32461 caaaagcaat aatttaaagg cagaaaaaat gtaaagtctt ttgtccataa aaacatcgga
    32521 aacttttgcg ttggcaagag agaaaccgac ggacatcaat gccaacggag tggcacaatt
    32581 ccccaaataa gtaattggcg ttttcacgat atccggtacc ggaatcccaa aaataaaaaa
    32641 caaaagggta atcagacagg aaattgtgcc catattgaga atgcttttca gattctcgac
    32701 agaaagtctt tttcccgttt tacctttcaa caaagcgata ccgtaggtat atatgacaat
    32761 attgaacagc agaataaaga tcgccacata aatggaagcg gaagggccgt aaatggaatt
    32821 gataagcggg ataccgatat aaccgacatt cggaaaagca agcatcagtt tatacatgat
    32881 ttgctgatct ttatcttttg aaaaaaaacc ggctgtcaca attccgatta ggatcaaccc
    32941 ggcaaacatg gcaattgcaa taatcgtaat cgattggagc atattatcct gaactcttcc
    33001 ccgattgtta atcacgcttg aaaaaaccaa tgccggattg aagacattaa tgatcagccc
    33061 ggaaatgcct tttgtttcgc tttcggtaat aactcgagtg cggccacata tcaaacccac
    33121 tacgatgatt atgaataacg ttaacatttg ccaaaagaca atttccatga tcatgagaaa
    33181 taaaactcct ttaccgcttg attccgggga tctcccctgc cgcaagaata ataatcccgt
    33241 ttcaagcaaa tcccagaaat tcagcgaaga atcggggaaa aagaatgatt ccaaccgctt
    33301 ttttctcccg ccgtaaacga aaaggggctg cccgagtttt cagacagccc cgatgatctt
    33361 ataaaagaaa aatcaggatt tcttttcttc ttccggatag acgactttaa tatgtaattc
    33421 ccgtaattgt ccttccatgg caggtgaagg cgcaccgctc attatatcgc gagcggcatt
    33481 gttcttcggg aatgcaatta cttcgcgaat gttcggttca tccgccatca gcatgacaaa
    33541 acgatcgatt ccgggagcga ttcctccgtg cggcggcgct ccatattcaa acgcatccat
    33601 aatttggtgg aatcgatcgc gagccacatc acgcggtaat ccgatcagat caaatacttt
    33661 ttcctgcagc catcgttcat ggattctgat ggatccggat gccaattcat agttattcaa
    33721 aaccatatca tactgatccc cgcgaacttt ccccgggtca gtatcaagta aatcaaaatc
    33781 ctctttcttg ggacgggtga agggatgatg ggttgcatcc caacgttgtt cttcttcgga
    33841 ccattcaaac agcggaaaat caacaatcca gcaaaatgcc aatttgttat tgtcacgaag
    33901 tcccaagcga tctcccagca tgactcgcaa tcgcgccaga cagtcataca ccacagccgg
    33961 cttgctgtcg gctacaaaca ggagcaaatc gcctttctcg gcttccattc gtttcaacaa
    34021 ttcttcccat ttctcgttcg agaaaaattt tttaaacgga gaactttctt ctcccgactc
    34081 actgattttt acataggcta accctttgcc gccccagagt tttacctggt cggtcaattc
    34141 gtccagttgt tttcgggtgt attctcccaa cgtttttgca ttgattccgc ggacactgcc
    34201 gccgtctttg atagcacttt caaaaacggt aaagccgcaa tccttcgcca aatcactgat
    34261 attcaccaat tccattccga agcgcaaatc gggtgaatct ttcccgaaac gatccatagc
    34321 ttcctgataa gttaatttcg gccaggggct gaacaggatc ttcttctgag gaaccaaatc
    34381 ttcgcacatc ttcgtgaata aatcttccat caaatccaga atatcatcgc gctcgacaaa
    34441 tgacatttcc aaatccaact gtgtaaattc gggttggcgg tcaccccgtt gatcttcatc
    34501 acgaaaacaa cgggcaattt gaaaatatcg ttccacaccg gcaacctgaa gcaactgttt
    34561 caattgttgc ggactttgcg gaagagcata gaattctccg gggtaaatcc gggaaggaac
    34621 caaataatcc cgagctcctt cgggtgttgt tttaaataaa atcggtgtct cgatttcaag
    34681 gaaatctttg gcatccagat aatcccgcat atatttaata atcttatgcc ggagaatcag
    34741 atttttttgc attcgttcac ggcgtaaatc gagaaatcga tactttaatc ttaatgtttc
    34801 atctgtgttt tcttctttat tgatcgaaaa aggaagtggt ttgcaggtgt tcaaaacatt
    34861 gatttcggtc gcatagactt caatatcacc ggtatcaaga ttgggattct ttgcatcagc
    34921 cggacgggcg cggacttctc cgattacctg aagaacccat tcaacccgaa cgtccgcgac
    34981 caacttggat aattcgggtg aatcttcggg attgatgact acttgagtta atccgaaccg
    35041 atcccgtaaa tcaataaaat aaatgccccc gtgatctcga taacggtgaa cccaaccggc
    35101 caatgtcaca gtctttccga catccgaaat tcgcagctca ccacaagtat gtgttttata
    35161 cataaggaac cccttttctt aaactcacat tcaaatcata aaaatacgtc ttttttatga
    35221 tttatgcgca gatattataa cccgcttttc tttcgttcag ggaaaaattg atcgatatgt
    35281 agagttataa aagatataaa atccggcgaa acactttttt tcagtttctc attcttttca
    35341 ccgattttat ctttacgaat cctcaaaaga aaagagggca tccgatgaag aatgccctcg
    35401 caaatcgata ttgaatcgct tacttattta catttgccgt aaaataaata ggacacagaa
    35461 tctcattatt tcgaaccaca ccccattcca tcgtataagt gccataaccc gacggggtaa
    35521 tgatatccaa gccgaaagtt aatgtctggc cgggatagac gtcactctta agatcgaacc
    35581 gaataccgtc aggtcgtttg agcatattcg ttccgccgat aaaagcgata tcaaaatccg
    35641 aagctgtcca aacggtgtcc gattcattcc gcaccgtaac ggtaaaatcg gtctcctgtc
    35701 ctgcataaaa agaatcggcg gaagtcggaa acatgctgat aattgaacag gttccgtttc
    35761 cgatataacc gggataggaa gtgggaacga aacccgaatc cgcaccttga ttgacaataa
    35821 tttttaccca aacactgtca tttcccgatt gtgtcgccgc ggttccgaac tttattcccg
    35881 aggggttccg cattttccaa aagccttgat aagtcccgtt tacactcggt gcttgcatgt
    35941 caacgctgat atcgatcgtt tcgttcggca gcacattgcg cggtaaatta atcgccgagg
    36001 gcgctcccaa ctgatatccg ctgtcgaaaa caaactggta actcgtcgtc caagtgcaag
    36061 tcccggtatt ttgaatgcgc cagacctttt gaaaaacttg attgggctgc ataatcgtat
    36121 tatcgggaac ggtcacatcc gccacaaaac ccatttgatc acaaactttt tgcacaggct
    36181 gaacataact gattgtcgga atcacaggtc gtgcggtcgg ttgcgaaatc acaattgtcg
    36241 gaattgcgat agtcggccgg ataaccgtcg gcgtggctgt cggacttgcc tgcggaatca
    36301 acaccacagc ggttttggtt gtatttgaag aaaccatcgc ctgaattgtc tgctgagccc
    36361 ataattgcac ttgctcggca gatagggtca ctgccggcgt tttgtttcct tcggattgac
    36421 atccggtcaa tataagaatc agaattatga tcccaagaat cgtctttttc attcaaccct
    36481 ccctaaacgg agtccctctt ttttatatat tcaataattc attaacgcat cactcgatca
    36541 tttggtttca gaagggaata ttttatcgtt tatccgattt caatcataac aagatttaac
    36601 cgccgtcaca ggatttttcg gaaaattctc ataattaagt tcattttatc cgaacatact
    36661 cttaaacggt catcggattt cgggataaaa attgactgat cacagaaaaa gggtaaaata
    36721 aaaaaaacag aaaataatat ttttcctttg gggcagggtg aggttgccga tgcagccgat
    36781 tcccgatcgg tggtgataat ccacgagcgc aagctgatcc ggtgcaattc cggaatcgac
    36841 ggtatagtcc ggatgaaaaa agggatgtga cagatcggat cctttcagaa gccccgaaga
    36901 aaaaggggct ttttttatgc aaaaatacgg aaacaaaggg aatacactcc gacatctaat
    36961 cccgccgata tcgatttgtg ccggtttgtg catttgggaa attcttgtcc ggattttcaa
    37021 ttatcccccg tttatattgc cggcaccctc tcgaattttc agccggttga tcgaatcgat
    37081 cgcaagcggc gtccttttat atcataccgg aattacattg gtcgaagttt tgaccggttc
    37141 gatcaccggt acgattctgg cattgctgac cggatatctg ttagctcatc atccgattgc
    37201 ggaaaatgtt ctgttcccct acatagtcgc gtctcaatcc attccgatgg cggcgatagc
    37261 tcccctattg atcatttggt tcggatcggg aataatgccg aaaattctga tttgtgcctt
    37321 ggtggtcttc ttccccattc aaatcaacgt cattatcggc ttacgtgaca ttcaaaggaa
    37381 ttttctggac ctgatgaaat caatgcatgc aaaccggaaa gcacaactga aatacctgga
    37441 aattccggct gcactgccgg tggttttcgg cggattacga atcagtgcga cgctttccgt
    37501 catcggagcc gttgtcgggg aactgaccgg tgcggatgcc gggttgggat atttgatcaa
    37561 taccgcaaga ggacagtacg ataccgcaat ggtttttgtc gcggtattta tgttgatgat
    37621 catttcaatt ttgctctatg tattgattct ggcaatcgaa aaacgaacct tacgttggca
    37681 gactgttcaa taaatcggaa aatcggagga aaaatgattt ataaaaccat taaaaagctg
    37741 ttatcggttc tattaaccgt cttgtttctc atgaccggtt ctaaccgagt ctttgcggaa
    37801 gaattctcat taccggtagg atttgtcccc aacgttcagt ttgctccgtt atatgtcgca
    37861 cttgaaaacg gttatttcgc cggggaaaat ctgaaaatca cactggatca cagccttgaa
    37921 acggataccg tcgcattggt aggcgcaaac aaaataccct tcggcatttg ctccggggaa
    37981 caagtgctgc tcgccagaaa tcagggactt ccgctgactt atattgcgga atggtatgaa
    38041 caatatccgg tgggaattgt ctcgctgaaa gaagcgggaa ttacctcaat gcaggatttg
    38101 cgcgggaaaa aagtaggtgt acccgttctc tccggagcca gctatatcgg actggaagcg
    38161 ttattacagc agagcaatat gacggatgcc gatattcagt tggaaacggt aggttatact
    38221 caagtggaat tgctgatcag cggaaaaatc gatgctgcgg taatttatat tgcgaacgaa
    38281 cccgaacaat tgaaagcact cggatatgag attaacctta ttcgggttgc cgatactgtc
    38341 ccgatggtca gtaacggttt gatcaccaat gaaaaaacca ttcgtgagaa cccggaactt
    38401 gtccggagaa tgacgcgtgc ttttctgaaa ggaattcaat ggactcgaca aaatcccgat
    38461 caggcttacg aaatctgtaa aaaatatgtc gataatttgg caaatgccga aaaccaggaa
    38521 ttacagaaac aaatattaat tcaatccata gcgttatatc aaagtccgtc ttccgaaaca
    38581 ccgctcggta caacggatgc ggccgcatgg gaaaaaatgg ttgaagttat gaaacagatg
    38641 aagctgctga agcccgattt tgatatgagc ggttcgttta ccaacgagtt tttaccgtag
    38701 ccggcaggta ctctttgcga aaagtgatcg cttccatcga aaatctcgga atgactttcc
    38761 catcggaaaa cggcgggctg caagctttaa gtgatattaa tctgacgctt tacgatcggg
    38821 aatttgtctg tatcatcggt ccttccggct cagggaaaag tactttactg cggatattgg
    38881 cggatgtgtt ggttcccaca cagggaaaag taacttttgc cgacgggcaa aatcccaacc
    38941 gtgccatcgt ttttcagagt tccaatttac tcccatggat gaatgtcatc caaaatatag
    39001 cgctcccgct taaaattcgg ggagccggcg aaaaagaagc gctctctcaa gccggaaact
    39061 ggattcgtcg ggtcggactg aacggattcg aatacgagtg gccgcaaaat ttatcgggtg
    39121 ggatgaaaca acgggttgcg atcgcccgcg ctttagtcca acaaccggaa ctgcttcttt
    39181 tggatgagcc gtttggttcc ttggatgctc tgacacgtga tcagatgggg ttagaacttt
    39241 tgaagacctg ggaaacgttt catccgaccg ttctgatggt aacccattcg attacggaag
    39301 cggttttgtt agcggatcgg gtagccgttt tcagcaaacg ccccggacag attattcacg
    39361 agttggaaat cccctttcaa cgcccgcggt cgcccgaatt gagggatgag ataaagttct
    39421 tgcaggtggt taaaaaatta agaggaatga tcgttaccga aagtatctga acaaagcttt
    39481 gcgaccgtaa aaatttttct ccggatcatg acggaaaatt cagcagcaac tcttccaaca
    39541 acagttgcgg actgacattt gcatccaaat attccaacga ccgattcaaa tgccggatca
    39601 attcgcgaaa ttgttcggtt gaatatttgg cggctaccga agccgattcc gaacagagat
    39661 caataaaagt gagcgattga gcggtcagtt taccgccggt agaaagcaac agtaaatccc
    39721 gcatgacaga ttgccaaaca tgcaatacct cccgtaattc cgttcttttt ttaaaatcct
    39781 taaaattgga agcatatcga aatcgttcgc gggtattctg tgagaacatt tcaaggcatt
    39841 ccgcggctgt tctattcatt tgttccgctt tttcaggttc ttttaagagc gaaatcgcaa
    39901 atccgacccg gccggcagac agatgagcgg cgctttccgc cgctttcgat tcgacctgat
    39961 atcgatccgt taaatagctt gtcaactgcg ccggattggc cggaaacaaa tgcatcactt
    40021 cgcaacggga tgtaatggta ggcaagagag aattttcgga gtcagctgtc aacaataatt
    40081 tcacattttc agggggctct tccaaaattt tcaacagagc attttgagca ttttgattgg
    40141 cttcgtcaaa gcgcagcaaa agaccgatcc gccagcggga ctcataaggc gccaacgata
    40201 aagtatgttg cagatcccgt atttcgtcga ttttcagtat ggttccggcg gtttccgtct
    40261 cggtcacaat caaatcggga tgttgctgtg cagcaatctg ccggcagttg cgacattttc
    40321 cgcagaactc acccggggcg ggcggttccg aacaatttaa agcctgtgca aactgaaggg
    40381 caattgtccg gcgacccgtc ccgggaattc cgacaaacag ataagcatgt cgaacttcat
    40441 cctgcaaaat atgattgcgc aggatattta cgacttgatc atgcccaaaa atatcccagc
    40501 tcattttttc attttctttt tcttcttctt ggacggcgaa gaagcctgaa caacttccct
    40561 ttcccgcggt tcctcttttt gggcggtttc gtccggagat atttgcccgt catccgatcg
    40621 gtccgaatcg ctttcaaccg gttcggcatt cgattcctga ggagacggct cttgttgtcg
    40681 taaatccgct aagctgatcc gctccgctgc cgtcagcata gataattgcg gtaaggtgac
    40741 aaacatttgt tccacaacgc tgcgacgaat atcattcaac agttgtgaga acagctccgc
    40801 cgctttggtc ttataaacca ccaacgggtt cctttgggca taactttcca tgctgatcga
    40861 aacacgtaat ccttcgattt gagtcaaata atcgacccat tggccggtaa tacttcgtaa
    40921 aattacctga cggtttactt cattttgaat tcgttttccc aaataggatt ggatttcagc
    40981 cgcgatttcg ccatccatct cagccggcgg aatttctttc acttcttcga aaatttccga
    41041 tccgattgcc ctgctgattt tttcctgggt ttccggtttt agctggctta aaggaatgtt
    41101 ataggatcgc aatcttcccc aatccaattt tccgaaaatc tgcctcaatc cgtcttcggc
    41161 attcatgtaa tgattccaaa tcaattcctt cagttcttcc cgatccaaat cctttaataa
    41221 atcaccggcc gtataaacca aattaatgcg cgtaaatatc cgactggttt ttcgatgagt
    41281 tttggcgtcg aaaccgatcc tttgtccctg actgatcgtc cgaaagagtt gaacccattg
    41341 tttgtccgtc agttcagccg gttggtaggt gcgtaaagcg ctttccaaat ccgacttcac
    41401 ctgtccctga tttccgaaca gacgttccgc ccgggtacga aaagccttct gaaccgcatc
    41461 cattaccgca ttttcgaaac cctcccaatc accgatctcg aatttggttg tcggaggttc
    41521 ataatcaccg atcatccgat ttccaatcaa ttgattcaga cgttgaatat agcccgcaaa
    41581 taaagcgatt tcaatttgac tgcgcaattc tccgatgata tcggcatctc cttcggctaa
    41641 tttgcgcagt ttgtccggtg aaagcttgaa tccgactctt gcaatatttc cggcttccgt
    41701 caacagatcc tgcggtcgaa tcggttcgac cactcgccct tcttctttgg cttcctgttg
    41761 ttctttgagc ctttctttca gcgaatcaac gaacagctca aaattagctg ttcgctcctc
    41821 cagttgactt tcataaccgc tctgagaatt acgaatgagc gtttccatct gctccaaacc
    41881 cacttcattt tctcgccgaa aagcctcccg gcttaattca tccagagcat ttaaaagatt
    41941 ctctttttcc ggggcggacc cgactttttc ttttaaagat tgaatcagca gagattgtga
    42001 gaaagagggc agataattct cttcaacctc aatcgtcggt tgaatgctgt ccaaataaag
    42061 cgcgagtttc cacttttctt catccattgc tttatccaga cgattatcca aatctgtttc
    42121 aagcatggca tgcacatctt cgcttaaatc ttctttgaca aaagcctgat cccgctgttc
    42181 ataaatccgc ttgcgctgcg agttgagtac atcatcatat tccaatgtat gtttccgggc
    42241 atcaaagtta gcgccttcaa cccgttcctg ggattgttcg atcatcctgg aaaaaaggtt
    42301 attctcaagc ggaacattca catccagtaa atttacccgt tttaatactc cttcaagctg
    42361 ctctccgcca aagagacgga caatctcatc ttgcagcgac aggaagaagc gggaagaacc
    42421 ggggtcgccc tggcgggctg cccgtcctct taattggtta tcgattcgac gagattcgtg
    42481 tctttcggaa ccgatcacat gtaacccgcc caaatcacgg actttctcca tctggtcgat
    42541 gtaatcgata taggctttaa ccgattcctc gtatactccg tattcttcag gcggaacttt
    42601 cagaatcgct tgataacgtt catccaaggt catgttatag ggatcgatgc ccattttcgt
    42661 caaaacacgg ttggtatccc gaatgcgttc ttcatccaat tctccgccca gtttaatatc
    42721 gacaccgcgt ccggccatat tggtggcaat cgtgatggca cccaaggcac cggctttagc
    42781 aatgatcatt ccctcttcat cgtgtttgcg ggcgttcaat acctgcggat tcataccact
    42841 ttcgacaacc tcaataaacc gatcgacatt cgattcgctc aacccgaatt cttccatcag
    42901 aagcgatcgg ttatccggat catcgacatt cagcgataca cctaactgtt ttgccatcgg
    42961 gcgtaaatcg ccggcattga tctcttggat gggtttatta aacggaataa attccttaag
    43021 cggcgattcc aaaacctcga tattattttg tttcatccat gcccggcgca gcattaaaat
    43081 ctgtaccagc cgacgcaaag gttcttgttt cagtcgactt gccaaatatt cggaatgttc
    43141 gacggaagcc gtaccgacca attgcggccg gccgattaca tgatttttca gaatctcaat
    43201 tgtgatcgcg cggaacttcg cctcctcact ctgataaatg acatccggat aatccttccg
    43261 cttatagtaa agcggaatat ccggtgcggc agggtcggca taataggtat atttgtaacc
    43321 ttcttcgtct ttcgcctcgc gctcgatcag gttactgttt ttctgtcgag cggtatattc
    43381 aaggttggtc gggatcggaa tgacatccaa cagataaatt ttatagaatt cttctttttc
    43441 ggtcagagcc gtaccggtca tgccggcgat cttgtcatac atccggtaat aattttgcag
    43501 cgtgatggtt gcataggtca tattctccgg ctcgaccttt accccttctt tcgcttcaat
    43561 cgcctgatgc aaaccgttgg accaacgtcg ccccggcatt aaacggccgg tggagctgtc
    43621 gatgatgatc actttccccg actggacaag gtattcttta ttccggtgat agagaaattc
    43681 ggccgccaaa gcctgattca gataccccat catccgttcc tgttcaattg tcacttcttc
    43741 cggccggctc ggatccctta ataccatccc caacaactct tcgacatgat ccatcccgtt
    43801 atcgttcaga taaacgttat gatcccgttc gctgacctca tactcatccg gtgacaattg
    43861 cttgaccact tttgccattt ggtaataagc atccacatca tccgaagccg gaccggagat
    43921 aattaacggc gttcgcgctt cgtcaatcaa cacattatcc acttcatcga tcaccgcata
    43981 agagtgaccg cgttgtaccc gatcttccag cttcattacc atgttatccc gcaggtaatc
    44041 gaaaccgaac tcgctgtttg tgccgtaagt aacatcggca tcataagctt cttttcgcgg
    44101 aaccaatcgt aaatgatcct gatcctcatg aggcgactcg atactgtagt cgaccaggaa
    44161 ggctttcctg ccgttatccg tccgcgccgc catctgcagc acgcccaccg tcaacccgag
    44221 cgcctgataa atacgcccca tccaacgggc atcgcgtctg gcaaggtaat cattcactgt
    44281 aaccaaatga acacctttcc ccgccaatgc gttcagataa accggaagag tggctaccaa
    44341 cgtttttcct tcaccggtct tcatctcggc gatatttcgc ttatgcaaag caatgccgcc
    44401 gatcaactgc acatcataag ggcgctgccc caaaactcgt ttgctggctt ccctgacagt
    44461 ggcaaatgct tccgtcagaa tatcatccag cgtttctccg tttgccaatc gatctttaaa
    44521 ttcatcggtt ttggcacgca atgcctcatc gctcagtttt tcataatccg gttcgagacg
    44581 attaatttcc gagacaatcg cggtatattc ttccaatttt ttctggatcg gatccaatcc
    44641 gagcgctgtt tttaacttcg taaacataaa tctcccacat cgggtgaaca aatcacccgc
    44701 tttcctcaaa attctgcgtt aatcttacca cttgaagcaa atcaaacaac aaaggctttg
    44761 tctcaaagag catcaaaact ccttcggaca aagtcaattt tttctgccgg ttaccatacc
    44821 caatcaaatc ggttaataag tcccaaaaat gatttcatcg gcgtattaaa tcggttcttt
    44881 ttttctctta tgaaatcatt ctttccggat atcacacttt caaatcaaaa ccttgatccc
    44941 ggacaacctg aaggattttt gctcgtttct ccgacggatt ctgcaatttc ttaatgactt
    45001 gcaaagtgaa gctgtccacc cgtcgattgg cttcccgtaa atcataataa gtttgcatct
    45061 cttcgtcata ttcccgaacg gcttctttat aatctgccag aattttgtat tcattttcga
    45121 aaactcttaa atcaagcggc aggcgtggtt ttaattgcgg attctggttc ggccatccga
    45181 atccgacgcc caataccggg aaagtcaact tcggcagctt cagtacctga atcgttgcgg
    45241 ccgaatcatt caagatactg cccagaaaaa cagcgcccat tccgagagac tcaatcgcat
    45301 tcgtcgcatt ttgtgccatt aaacaagcat ctgtccaacc ttgaaagaag cgatccatat
    45361 ctcgttcgga ttcaaattga taaccgttgt cttctgcgat ccgtgcattt cgatagacat
    45421 caacgatgaa aataatcaat tcggggaatc tcgcgacata cggttgttga catatttcgg
    45481 ataatgcctg tttcttcgcc atatccgtca ctcttatcat agcggctgat tgcataccgg
    45541 tactggtagc tgtatggttg gcagcttcca aaacgagctt taagtcctcc ggcttcaccg
    45601 gttcgtcagt aaattcgcga atggagcgat ggttcaattg atgggtaatt gtttggttca
    45661 tcatttcttc ctttatttat tgattgtcaa aaataagttc atctttaatt ttttgcgctt
    45721 cttcttgcaa atatatccaa tccatttcgt tcaaaggatt ttgcaatacc cgatcgatat
    45781 gatcgagcat gaaattaaaa cttttaaaat caccgggagg gacaagagcg gtcgctccct
    45841 tggagaatgc atatttcatt cccgccattc cggtcttttc gttatcggtt gtcggatgac
    45901 accatgattt tgggaattcg tcacggttct ctccttctcg ccatttccgg tcgaccagcg
    45961 tcttaatgca gagcaaccct ttgtcacttt cgcgaacttt ttgagccaca cggtctcccc
    46021 atccgcgaac caatcccaaa gcccagttca taggaaacaa cacggtatca aaatcaaata
    46081 aatccatcgc ccttaatgct tgttcttcgc tgtgagccga aaaaccaatg tttaggataa
    46141 tgcctttctt ttttgccgac aagaatgttt ccatcgcccc atccgggccg aaaatggtat
    46201 cgacgtcttc cgcagtggtt atggaatgaa gttggtagac atcaaaatga tcggtttgaa
    46261 gggtttttaa agaattctcc aactctctga gcgatccgga tgcggtacgt tccatcgtct
    46321 tacatgcgag aaaaacattt tttcgataag gtttcagcgc cactcccaac aattgttccg
    46381 catcatcgta agaaggtgcc acatcaaaat aattcactcc gtggtcgaca gcataactta
    46441 cataacggtt ggcggtttcc tgagtctctc ccatatgaat gattccccca tatacaaccg
    46501 ggctgatcat catctccgtt cttccgagtc tcctcttttc catttcaatc tcctttgatt
    46561 gacagctcat tgcactatca aatgatcgct gttgatatat ttatattgta aagacaaata
    46621 cgattttttg aatagacaac tgtttatcga taacggattt caaaacggag agaattcagt
    46681 tccttaaaat gaataagaaa aaagccgcat aaatattttt acaaccactt caattcattt
    46741 tgaatcaaaa ttccgacact gtcggcaaat cgttcaagcc ctttgattcg tacgaaatta
    46801 tgtccgtaga ttctttttgc ggaaggcagg tcctcatcca ttccggtaaa tacgcacatt
    46861 atggaaattc cgaaatctct gctcttccgc acttccgccg ccgtatcatt cagcccgacc
    46921 aaatccgtat aatccgcttc gacctgagtt tttcccgttg tcgggacaat gttcgtgtcg
    46981 agtggttttg catccgacaa aatgatcaac aatttgtgtt gatagggtga atttttcatc
    47041 atatgaatcg ctgtccggat agccagccca tcccgattac aaccggaaga aaagtaatta
    47101 aaaatcttat cgttatcata aatttcatca taatctctga aaagagtcat gatcgtaaat
    47161 tttcttttcg tacaaaacga ataaacacga acagggattt ggcagcgtgt caaactttcg
    47221 gcaatgataa atccctgtgt cgctatcgcc tcctgccggc cgagctgtga agcggaagca
    47281 tccaataaaa tatccaccga caaatcaccg atttcgtttt gaattttttt ctgaaaaaca
    47341 tttttatcgt gaatgtacaa atttctccat gcccgttcgg gcgtcaacct tccggcattc
    47401 gctctacccg aaaaagagga aggcatcaac agcatggaat ttcgaatttt ttccgtcaat
    47461 ttaataatac cggtattatt gacagccaaa ttcttccgaa aataagccct gtttttctcc
    47521 cgctgcatca gaataacgga ttgttgttcc gacagattcc cggaaacatc aatttcaccg
    47581 cgggtaaaat gcaggcgaca gtttttatgc gccccggtac aaagcttttt ttcgagaatt
    47641 ttcacctcag agtccgagag tacaggtttg ccgtaatatt cacggatata cttttcctga
    47701 tttatctcgt tggcatttcg gtatttgcgc cagtaggaag tcagtatatt ttttctcacc
    47761 ggaacgcgat cggaattcgt ggctaagggg aattccaacg gctcgttctt cggattccgt
    47821 aaaaagggaa tccttagaaa tctttttcga tttgtttcat tccattgata gacagtttta
    47881 ttcttttgca ggaaagccaa tcggtttttc tcatattcgg ctttttgaaa ttgaaaataa
    47941 ttcaaaataa cagcgtttaa cttttcgatt atttgttccg tcgttaaaga ttcatcaaat
    48001 tccaattgat ccaagacgtt tgctaccgtt tcggacatta ccggttgttt tcccaaaacc
    48061 cgctgaaaat gggctacttt gagatcatcc aaaatatcgg aatcagcggt tacggaataa
    48121 ttttccaaaa tcgtatttgc aaagtttcgg cgtaattctt caagtaccgg ccggtctttc
    48181 ctgcctttca gaaaagtact gttttccaat cccaaccagg tcaatccccg aagaaaatcg
    48241 gcatccgcat cctgtttcag cttttcgaag aactcctcaa gcagcggaaa atcataataa
    48301 taatgtgccc ctccgataat ataattccca tataattcgg ctttcccgtt ttgatcgaaa
    48361 tatttgactt cggggcgaaa agagtaatca aacgccgcat tccaaataat attggatgct
    48421 cttcgatttt cctgtaattc cgtttttatc atcaatcctt aaaaatcgca tccctttcta
    48481 aaccggcagg gattctgagc cggatcacat cctcaacaag ttttcgttcg aaatcatcaa
    48541 aggttttatt ggtgattccc atttgtaaag ctttatcggc ttccaatccc ctatccatta
    48601 aatgaattgc agccaataaa ccgcgcagat ccacaggctt cgtcgaaatt tcggagttct
    48661 cactcttttt ttgcagatcc tgaaataatc cggagaactg ttcaatatat ttctcgttaa
    48721 tggtcggaaa ttcacgcagc aataaccgtt tcagattttt ttgagagata accggcattt
    48781 caatcaccat gaatcgagat gtcagcgctt cgttcagttc ccgtgtcccg gcataaccgt
    48841 agttcatggt cgcgataaag cgggtcgagt ccgccaaaat aaatttatca taccccggca
    48901 catcgatgat ccggcgaaaa tccaaacttg catggagcac tgccatggct tcgttcttcg
    48961 ccatattaat ttcatccaaa attccgaatc cgccccaacg cgcacattca taaatcggcc
    49021 cgtgtcgcag cagcactctg ccctcttcca acgtatcaat tccgatcaga gaagaggaat
    49081 ctgtattaat atggaacgaa acatcccagc tgggtctgcc gaaagctgcc gcaagattct
    49141 ccgcaaaaac gtttttgccg gtggctttgg gcccgaccaa taaaagattt tcaccgcaga
    49201 gaagggctgt aatcgcctcc tcccagatat catttccata ataataatat tttggttggg
    49261 gaattcgata ttttttttcg ggacttaccg gatatttttc tcgaaaaagc tcaatttgag
    49321 agatcagatc ggaacggatt ccttcctttc tcaaaaaatc cagcatacag tatacctccc
    49381 ataatcggga tatgtattaa tcatcctttt cattataaat acaaactcaa ccttctgcta
    49441 taggaaagga gaaggttgag gaaaaataat cgatcaaaat tcttttcggt taaaaattcg
    49501 aacggaaaga g
//
LOCUS       DAOSMM010000010        46744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_833_length_46744_cov_15.618433, whole genome shotgun sequence.
ACCESSION   DAOSMM010000010 DAOSMM010000000
VERSION     DAOSMM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 46744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46744
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_833_length_46744_cov_15.618433"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..616
                     /locus_tag="PK898_02875"
     CDS             284..616
                     /locus_tag="PK898_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19008.1"
                     /translation="MEQKSNIKALIIIVNAGFSNEAIKIARESGATGATILNARGESL
                     RHEVFMGISVDSEKEIILCLVEEIVADKIMSTIQEKAGIKTPAHGICYTMGVEKIIGI
                     NSFSSQAN"
     gene            826..1890
                     /locus_tag="PK898_02880"
     CDS             826..1890
                     /locus_tag="PK898_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU19009.1"
                     /translation="MAFNPLNWLMGLFSLDIGIDLGTANTLVYVRGKGIVINEPSWVA
                     VEKRTRRPVAIGAQAKEMVGRTPANLMVVRPMRDGVISEFEITQAMLSYFIGKAHQQT
                     IAPFPRPRVVIGIPCGATDVERRAVYDAAMAAGAREIYLIEEPKATAIGIGLPINEIR
                     GTMVVDIGGGTTEVAILSMNEIVVSRSLRVAGDEIDQDILNYIRNRYNLYIGERMAEQ
                     TKISIASAYPLPVERTMVIRGRNLVTGLPEAIEVSSVEIREAISGSIQVIVDTVKDAI
                     DEAPPELVADLIETGICLSGGGSQILGMADRLNDELNIRVWVAEDPMTCVARGAGMVL
                     ENSSTIEQFAASLDRFTPKK"
     gene            1987..>2330
                     /locus_tag="PK898_02885"
     CDS             1987..>2330
                     /locus_tag="PK898_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19010.1"
                     /translation="MNNVKKNWQKIVATLIISGVIFLALSGFLTQSLSGLIDPIIQVQ
                     KWTAERIQTVYEFFTIPRDVTQLRAENQALQEQVSLLQTEIIQLQQDLKEADILYSLL
                     GFARGRPEETYIA"
     assembly_gap    2331..2430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2595..3098
                     /locus_tag="PK898_02890"
     CDS             2595..3098
                     /locus_tag="PK898_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19011.1"
                     /translation="MKEIISSFVILILGGILQGSLFSRIHILNGTGDVIMVMLIVWSL
                     NPYTRYSWIWLLLGALIMTYLSALPLYGYFFIYGIIWLFIRFLKSRFWQMPIILMIFV
                     TITGSIISAVISYFVLYINDISPNLKTTVTQIAVPSLVLNLIYCFPIYGLLNDFANSV
                     CYKEESS"
     gene            3098..5191
                     /gene="mrdA"
                     /locus_tag="PK898_02895"
     CDS             3098..5191
                     /gene="mrdA"
                     /locus_tag="PK898_02895"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU19012.1"
                     /translation="MNEFNREKLDFKNRRFVAFYVVMGAIILFFIYRLVVIQLVEGDY
                     YLSLADENRISIVKEQTTRGIIYDRNGVVLARNVPTYDIVITPANLPTDEGDIQAIYR
                     KLSEILDIPVNNGVINEETVRLYSECGTDFGISQIIYIANSLTPYAETAIKCDVPEET
                     AMIVQELAANMPGVGVRVNSIREYPTGYDTSEVIGFLGPIPDTVADEMESRGFILNKD
                     KYGYAGVESSLQDLLSGKNGRRVVEKDVAGLETRDLEPPKDPVPGYNVKLTIDVRLQN
                     VARAAMLDTMDFYNRISVTGPLTHDGAAIAMNPQTGEILALVSEPTFENNRMTRYIPA
                     YYYQQLSLDQSKPLLNHATQVAQPPGSVFKIVTGIGAINEKVVSPDFHINCPGHIFIQ
                     NSYSPNDPGSEQEYVCHLRTGHGQVDYLHALAWSCNLYFNKVGGGYKDEVPEGLGIDR
                     LGQYAHALGYGEISGIELTGEETGMIPTRTWKRVNKGESWATGDTYLASMGQGYISAS
                     PLQVLGSFVTVANKGIHIKPTIIKEVLDDRGNVVLPFTPKMLWDITKDPKIMVYDENG
                     EETGKYKTVDPTVIDLTRQGLRLVTQPGGTASEAFAGDTHQSSAKTGTAEYCDDIAMA
                     KDACKFGNWPSHAWTVAYAPYDNPEIAILVFVYNGKEGAYMAGYPVRRILDNYFLLKE
                     YDAERAGETPGSLIQ"
     assembly_gap    5352..5451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5452..6099
                     /locus_tag="PK898_02900"
     CDS             <5452..6099
                     /locus_tag="PK898_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU19013.1"
                     /translation="MMVIWFSMLWVSKIPTKYLIILGTIGVILLIGITIWILVSPDTI
                     PFIQEYQLNRLKNFFNPDENARYGDKYNVDQAVISLGSGKLFGQGYGNGTQVQLRFLK
                     VRHTDFIFSVLGEEFGFIGTIIVIFLLVFVILRCLRAARLAPDLFGSLICYGVATLIF
                     FQMAVNIGVNLQVLPVTGLTLPFISYGGSSTLALTIAIGLVESVATRQEKREEIV"
     gene            6155..7660
                     /gene="gltX"
                     /locus_tag="PK898_02905"
     CDS             6155..7660
                     /gene="gltX"
                     /locus_tag="PK898_02905"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOU19014.1"
                     /translation="MTNEMKPVRVRFAPSPTGRLHLGGGRTALYNYLLAKKTGGKFIL
                     RIEDTDRKRYIENAEQELMDGLHWLGIDWDEGPDKPGEFGPYRQSERKEIYLKYAEQL
                     IKQGDAFYCFCTPERLHRMKEEQEKRKEFPHYDGQCRDIPLEEARKRIAAGEPYVIRF
                     KTPKEGVTTVHDAIRGEITVENRTIDDYIIVKSDGWALYHLAAMVDDHLMGITHVIRG
                     SEWIPTLPLHHMIIQAFGWEEPVWIHLSVFLKPSGKGKMSKRESADLIKDGYSIFLTD
                     FKDLGYLPEAVTNWIALMGWSYDDHTEFFTMEDLIKDFSIDHLNPSPAAINFTKLDYF
                     NGVHIRALPVEDLAHRIKPFFEAKGYHPDDETLLKITPIIQERIVTLDDAPEIAGFFF
                     EDHVTPQTEDLIQKGLTAEKCAEIAEKSYQVLADLPEISPRIGEPEMRALVEELGLKP
                     AQVFGVLRVAVTGRKVSPPLFESMEIIGKDIVLERIKTAAKMLREYQSRNN"
     gene            7685..8860
                     /locus_tag="PK898_02910"
     CDS             7685..8860
                     /locus_tag="PK898_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU19015.1"
                     /translation="MKNFSFQIPTQIIFGSGTLEQLNTISLPGKKAMIITGGTSVQKY
                     GYLDQVYKILKYRNIDHVLYDKVVPNPIRKNVMEARDIAREKKCDFFIGLGGGSSIDT
                     AKSVAMMMTNPGDLWDYVPVGQGGKKDFTQKGFPLIAIPTTAGTGTEGNPTSVITNEI
                     TGEKVGIRCEFPIISIVDPDLTININPRYTTYQGFDALFHNIEAFISQKANPFSDLLA
                     LEGVRLIFENLPEAVRNGKNIEARSAVSWAATLGGMVLCLASATSAHVIEHSLSGIDP
                     TVIHGEGLIMISKAFHQLGAERIPELYSKLAFAIGMGDSKKTVQENAKAFLDALEALK
                     IACGVGGLNLSDHGFTEDDIDRIAEGAYVIGGGALTRDQYKISDQDLKEILRNSMHD"
     gene            8853..9578
                     /locus_tag="PK898_02915"
     CDS             8853..9578
                     /locus_tag="PK898_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU19016.1"
                     /translation="MTESVLITGSVRRLGKIIACHLADLGYHVWLQYLHDDMEAENTC
                     QQIHRFGGAASLIKADLQNVDEIIRMFDFIRENGGLTTLINNAAIFYRSNLATLSVTD
                     WDRLFDINLRAVWLCSREAAKLIGENPTKSGLIINMSDSGSYQYWKEYGGYTLSKAAV
                     RDLTILLAKTFAPKIRVNSISPGLILPEESMDEKTWKHLSSETLLKKSGEPEDILNAV
                     DYLMKSDYITGIDIPVDGGYRWK"
     gene            9579..10517
                     /locus_tag="PK898_02920"
     CDS             9579..10517
                     /locus_tag="PK898_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19017.1"
                     /translation="MMQSKFYRQLQMILKVVPWWKIPISLGVMLSTVEFINFWDVPIN
                     WTAFVLIFLITIISLITANLLQMRLYIFSSDEKSIRFLNVPWGKPIEINKNEKDAVSV
                     NLIIYFLFFLLVAVYMSFLFLNKKNQWNLNFVFLLMIISILIFSFAGNSPRINWSEEL
                     LDGLLTVFFPSVLVILGHQNYPFQKYILSYLIPDFCFFLAFRFILGMSMLDYQVSFKP
                     GFFVNYLQKRKFRLHHLFYFLAYFSLILMNRLGVSWELLAPQLLLLPSVSFQIVLIEK
                     IMKGSPVNWKLLGWLSYLNLILLLYFQCITIVFYGF"
     gene            10599..11648
                     /locus_tag="PK898_02925"
     CDS             10599..11648
                     /locus_tag="PK898_02925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU19018.1"
                     /translation="MKIRSCKYVILALLAFFVTSCNMPERNVDKNVLYTIAASTIQAE
                     NNRIASLTPPTPTSTLTETPTITFTPTITSTPTIPPTATWITHPAGSAEVLILYYNDV
                     ANNANEDPYYQWESPLNVPSVEFEQQVRILYELGYTSIGLSQLVKVLLEGGELPPKPV
                     MFTFDSSKLGQYKNAYPILKKYGMAGNLMLVAAQVDTKNCLSSEQIKEMMNDGWELGS
                     SGYDGNHDPAYYGQEIGKSKILLEEKFGVPIEVFAYPGGIPDAGGVMASKVSSSLYKA
                     AMGKQHVTKQSRDNLYYLGRFEITKGLPLNDFLSYLPWKEGNISEETMNWTLPTVTFT
                     PPAALLQTQETPAAP"
     gene            complement(11800..12195)
                     /locus_tag="PK898_02930"
     CDS             complement(11800..12195)
                     /locus_tag="PK898_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOU19019.1"
                     /translation="MLKVSRRLNYGLQLMLALASDPNNTSKSTAAISESLSIPLPFLH
                     QIAHTLQQNGLIKATPGPKGGLRLGSKGLDITLYDIFNALEGQFSVPTIETDIDTSEN
                     DVAMGLWKEIGTKINNILKEYKLSDLVES"
     gene            complement(12356..12673)
                     /gene="rsfS"
                     /locus_tag="PK898_02935"
     CDS             complement(12356..12673)
                     /gene="rsfS"
                     /locus_tag="PK898_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU19020.1"
                     /translation="MVEILENHKAEEITLMDVREQVDFTDYFIICTGTSDRMLQSLSD
                     AVKNEIREKYQVHCIVQGIAEGGWMLLDIGDIVVHIFSPDRRNYYSLEELWNESKILL
                     KLQ"
     gene            complement(12736..13329)
                     /gene="thpR"
                     /locus_tag="PK898_02940"
     CDS             complement(12736..13329)
                     /gene="thpR"
                     /locus_tag="PK898_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOU19021.1"
                     /translation="MIKQNATLRLFIAIELPEMIRKPLSDSLKDSVIIKKKKARLTSK
                     ENLHITLKFLGDVQQDKRGDLQTAIANSIGGIGPFELKIHSGGVFPTIKKPRILWAGF
                     DISKELSVLVNNLENEFEKIGYSKDLKPFQPHITLARIPDYVSTMIPVEKDEIIKNLE
                     KIDLPPFTVDKISLMKSILLPGKPEYQVISTQNLKVC"
     gene            13549..>15299
                     /gene="alaS"
                     /locus_tag="PK898_02945"
     CDS             13549..>15299
                     /gene="alaS"
                     /locus_tag="PK898_02945"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU19022.1"
                     /translation="MEKKLSGNEIRQQFIDYFVERGHTAVRSASLVPAEDQTLLFTNA
                     GMVQFKDVFLGTDVRPYKRACDSQKCMRVSGKHNDLDDVGRDDFHHTFFEMLGNWSFG
                     DYYKKEAITWAWDLLTNVWGLDKDILWATCFRDEKDEIPTDEEAFEIWNTQPGMNKDH
                     VLYFGRKENFWEMADTGPCGPCSEIHIDLGESHCNKKGIPGHICHVNGDCGRFLELWN
                     LVFIQYNRLNETTLNPLAQHFVDTGMGFERIVSILQKTDSNYKTDLFLPMIREIQKLT
                     KVSDDEMYSNFTPYRVIADHARASAFLIADGVVPGNIGRNYVCRMIIRRAVRFGYQLG
                     LKEPFMSKVAEKVIENYGDAYPELNRMKESILHSVSWEEERFNKTLENGINQLNEIIE
                     EMKKGGQNIIDGETCFNLYSTHGLPLEITYDIVREYGLDVDRKGFKKASEAHRIASGA
                     GKIFGKMGGEDAEQYGMHLSFLKESKLLGEEGVICDPYQTKDVEAKLIMALVDGQRVE
                     KIEEGQFGELIVSQTNFYMEMGGQVGDNGWIRSEDGSFEFKVEGIRRPVAGLTVHYGK
                     AESGSISVGETVKVVVD"
     assembly_gap    15300..15399
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15400..16035
                     /locus_tag="PK898_02950"
     CDS             <15400..16035
                     /locus_tag="PK898_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOU19023.1"
                     /translation="GTHVSNTGEIGLFMIMSEGSVSTGIRRIEAITGRTANMTARKNT
                     QIVKNLASILNASTEEIFEKVENLQTLSNKYQKEIVQLQTQIALKDFKKFWETAQKIK
                     DAYVLTAMIPDSTIESLRNMADQFKAENPNAVVVLGTKTDDGQVQFIAAVSDSLIQRG
                     INAGEIIRNISALTGGKGGGRPNMAQGGGKDINKAQEALDAAVKMVREKLR"
     gene            complement(16188..17048)
                     /locus_tag="PK898_02955"
     CDS             complement(16188..17048)
                     /locus_tag="PK898_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU19024.1"
                     /translation="MPFHILVDSGSDLPKSLTNSYQNFSLLYLSYIIDGEPGPIDMPG
                     EEFYRRLRNGSMSVTSQINPEAFIEEYERLIKEGQKEILYIGFSSGLSGTYGSGKMAA
                     DTLSKKYPDCKIFTVDTLSASMGYGLLVDYAIRQRDSGKSIEQVRDWLEENKLKMNHW
                     FTVDDLHFLERGGRVSASSAWFGSALNIKPVMNMDDAGHLIPREKIMGRNKAIKRLYE
                     KMKEMAVEPQNQRIFISHGDCMEDVEKLEKLIHDNLGVNDFTVNYVGNVIGSHTGPGV
                     LALFFLGNHR"
     gene            17287..18183
                     /locus_tag="PK898_02960"
     CDS             17287..18183
                     /locus_tag="PK898_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOU19025.1"
                     /translation="MLKVGYIGLGIMGKSMARNILHGGYPVMVHNRSREIVKELVSEG
                     ATEAFSPAEVAANTDVVFTNLPDSPDVLDVVFGERGISEGGKPGLIYVDHSTIKPAVS
                     RQIYEKLAEKGIKSLDAPVSGGDIGAKQGTLTIMVGGDDFILEKVKPILSLCGKTITY
                     VGGPGTGQIAKAANQIMVAAQMVAMAELLIFSQKAGADCKKVVEAIKAGAAQCWTLDV
                     KPQRLFEGNREPGFKAYLQAKDLNIIMETAREYGIPLPSAAIDAQLYNAMLQLGNGQQ
                     DNSAIISVIEQLAGVHLNESNH"
     gene            18195..19286
                     /locus_tag="PK898_02965"
     CDS             18195..19286
                     /locus_tag="PK898_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU19026.1"
                     /translation="MNKRKSIGLLFVLLLICQLFPDFKVFADSDSSSEIYQSETPYNM
                     VFIDAGYSTVGAQQQDLNAKEDAKPLHIVYLDPYYFDILEVTNGQYAECVAAGACKEP
                     EVKSSETRDPYYGVDWFSRFPVINVTWQDASDYCEYVGKRLPTEAEWEKAAMGAEDYR
                     RYPWGNSDPKPYTINMTKVPGDTEMGNSYPKGASPYGVVDMVGNVSEWTSDWYSADYY
                     SVSPEKNPTGPEEGTEKVVRGDSFKTDIHSIHITNRYAMDPKTANNYTGFRCAKDVRE
                     LVEYTITPQPEETITTIKKAIVNSGQDEGIFVLEEPGVGKKLNCIAKNGSVVEVSEGP
                     KEMAYTQWYKIRTQSGCEGWSLATSLNLQ"
     gene            complement(19293..19808)
                     /locus_tag="PK898_02970"
     CDS             complement(19293..19808)
                     /locus_tag="PK898_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOU19027.1"
                     /translation="MTESWQKQLIPVRKQLLAKKNNPNVVILGIGNEVKGDDFAGSLA
                     AKSIQASLPQQTGNFSISVFDCGPVPENFTGVIRKISPDYILIIDAGDFGEDAGEIGF
                     FSWKKAEGSEISTHALPLSLFSGFLAAELDCEIGLLLIQPKNLSYLDPVHPLISEAVN
                     KISSEIIKIFS"
     gene            complement(19738..>20222)
                     /locus_tag="PK898_02975"
     CDS             complement(19738..>20222)
                     /locus_tag="PK898_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU19028.1"
                     /translation="LITYPTYDIDSGENTMKLIGMLSDVVSALFKRPATTKYPYVKTD
                     PPERYRAKLAYTASNCTGCMQCVRDCPTRTIKIIVVDRKTKQFVMTYNIGECVYCKQC
                     VYNCKFDALHMTNKMWESAAGSKGGFQMYYGKKEYIDRILAETTNPGAQAAVSEEKQS
                     "
     assembly_gap    20223..20322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20323..20858)
                     /locus_tag="PK898_02980"
     CDS             complement(<20323..20858)
                     /locus_tag="PK898_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOU19029.1"
                     /translation="MSAPHPLICLFFFPGGLFLILAALLYEWADRKLVARFQNRVGPR
                     VFQPVADMLKLLVKEEVIPKSVNRSLFIVLPIISFACVMTASLYVPMFGVEAPIGLHG
                     DLIITMYLLSVMTMCLGMAGANSMNRFAVVGATRTFTQLFAYEAPFMLALLMPAISAQ
                     SWNINVISEYASAHTWMIA"
     gene            complement(20855..22063)
                     /locus_tag="PK898_02985"
     CDS             complement(20855..22063)
                     /locus_tag="PK898_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOU19030.1"
                     /translation="MSDETVNNFNNNGQQKYIVPIGPQHPALKEPGHFEFTVDGEIVV
                     GASIRLGYVHRGVEKAAEQRTYTQDLYLLERVCGICSHSHGTAFIQCVEKLAGVELPP
                     RAKAVRCIAGEIERLHSHLLWLGVAAHEGGFDALFMYSWRDREIVMDILENFFGNRVN
                     YSVNIPGGVKEDITQEFIDGVTPKLDYLEKRMYHYLDVVNTDMTFRQRTVDIGTIGLE
                     ESDRLGATGPTARASGVARDIRIDRPYDGYDLFPIDITLDNRGDLNARFVVRIKECIA
                     SIHYIKNVMNHMPEGELSVKIPRKIPTGEYMASVEAPRGELFYFVRSNGTESPDRVKI
                     RTASLCNWGTIVSSVAKGHKLADMPMILAGIDPCFSCNDRMVYINKGSGNPEIWTWEQ
                     LRKYGIAYYK"
     gene            complement(22063..22554)
                     /locus_tag="PK898_02990"
     CDS             complement(22063..22554)
                     /locus_tag="PK898_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOU19031.1"
                     /translation="MEKEELLNQFQEIFTDLAIKTEIPAIQPNRVDVYINSNDLLKAV
                     QKLVEHDWGYLITISAYDTADENGTPQIGLVYQFGEQNVIGTLRFFLPYSNLTVESIC
                     PIIPSATLYERECMELFGIDIRNTPDRSKLILPENWPDGVFPLRKSFSGLQDNPETKS
                     GEE"
     gene            complement(22554..23138)
                     /locus_tag="PK898_02995"
     CDS             complement(22554..23138)
                     /locus_tag="PK898_02995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOU19032.1"
                     /translation="MNKTIDLSDIDPNAKGSEKTIENRIRNWARINSPWAIHYNSGSC
                     NGCDIELLTLITPRYDVERFGVKLQGSPRHADILIVTGSVTLQSKDRLIRIYNQMPEP
                     KFVLGLGSCAISGGVFQNCYSVVQGGVSNVIPVDVFVPGCPPRPEAMINGLIILLDIA
                     RGKRQKSDYSQPMIIPSPEQDSAQTADKKESEAE"
     gene            complement(23181..23495)
                     /locus_tag="PK898_03000"
     CDS             complement(23181..23495)
                     /locus_tag="PK898_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19033.1"
                     /translation="MDILLSPPIGFLIYAIIGTLLVFYGKKTAPVEDTKDDQKRTLYG
                     SGEVAPETRGVPGYKPFLLISLFFALLHLGVLIMGSSNLSVTSLVYTVVLMLGLLALM
                     LG"
     gene            complement(23508..25010)
                     /locus_tag="PK898_03005"
     CDS             complement(23508..25010)
                     /locus_tag="PK898_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU19034.1"
                     /translation="MNPLFWLLITPIICALILYVVGRTIPPDDESSPNKAAWLSLFSI
                     LLTVVPFVMTAKKFTEEGPVSLTAGMITLTFDGISLLMTSVLLVLSAMVVLFSAQYMV
                     AEDHEEKFYGQIMILMSSIMGLTTATDIFNLYVWFELMAVSTYTLVSFYRNRTESLES
                     GIKYLVQSAAGSVLVILGTSILFGLTGEVKISAIRESILTSSPAVIAATACLLIGYGV
                     KCAIVPMHFWLPDAHSQAPAGISALLSGIVIETGLIALLRALSAVSHAHLPLGTIFLV
                     IGCINMFIGTFSALRQKEIKRMLAFSSVSQMGYILLGIGFAISFPASAQNAEGIFTHI
                     FNHALMKGLAFLSAGALVYCIKVSKGNHEPLLLDDLNGVSAKYPLYSLAFTISMLALG
                     GLPLFAGFISKWQILASGIAAQKSWVAFLIALAGINSVLSLGHYAPPINRIYRHVSNN
                     NEVAISPKFQQAMIIPIIILTAAVILLGFFPGITAGITKVASETLYTMFY"
     gene            complement(25018..26775)
                     /locus_tag="PK898_03010"
     CDS             complement(25018..26775)
                     /locus_tag="PK898_03010"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOU19035.1"
                     /translation="MAGILIPFLIFTPWLTAILVWFIGDKHEEGLHQFASAGAILSAI
                     LSLLLLSQIGDSTISFAKIGPLFGNLSFSTDALGIILACIANCIGSLTVIFSAEYMKH
                     EKSLARYYSMVLLFIGAMSGLVLTANIFMIFFFWEITALCSYQLISFYNDDPNAVRGG
                     LKALFITQFGGIGLMVAGLLIFGYTHSFEIGRFIQAAQGGEIPANVLGIIGFTLIIAA
                     TAKSAQFPFFTWLPDAMEAPTPVSALIHAATMVNAGVYLLCRFFPALEAVPGWKMTIL
                     ILGLITILISSLMAIFATDLKRVLAYSTVSQLGYMFAAIGAGSVLACQFHLLSHAIFK
                     ALLFLCAGAIIHSCGTRDMYKMGGFFKKIPFISITFIIGSLALAGIPVLNGFWSKELI
                     LESIHSGTPIIIYILMLFSVILTALYTIRCVWLVFFGEPRSDLHVHDAPFLMALPLGI
                     LSLGTITSWLVFEPFSRSLSNSLPLYGIPKETLSELVSKIFGDPYTFLALGMVAIGIL
                     IWFLRNKLPLHNSITRKIKSISLNSFGFEAINNGVVNGINAASEAIRNIQTGSLNWNI
                     FGMLIGLICILIALMIGGL"
     gene            complement(26779..27078)
                     /locus_tag="PK898_03015"
     CDS             complement(26779..27078)
                     /locus_tag="PK898_03015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOU19036.1"
                     /translation="MNTLVIIAIVSLIAIGFYCLIITRNILKIIVALQVLVKGAMIAM
                     ILAGKLTDQMELAQSMAITIIVADTIVAVIGLAMSIQLREKTGTCDIHSISKLKR"
     gene            complement(27075..27575)
                     /locus_tag="PK898_03020"
     CDS             complement(27075..27575)
                     /locus_tag="PK898_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19037.1"
                     /translation="MPLVYFLVVAALIYSAHKATRVKGLLNAALWLAGTSCLTSILVY
                     ILGSPGLAVIELSVGAGLVTVLFVFAISIIGDEKITSDVFVPHWLAISISVVSFGMLA
                     YLLIAQHMPSVQSETTSPIFSDLFWEGRKIDIYLQGALFISGILGILLLIAPAVSKRI
                     KEGKNK"
     gene            27903..28469
                     /locus_tag="PK898_03025"
     CDS             27903..28469
                     /locus_tag="PK898_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU19038.1"
                     /translation="MKYLLKLDEKYSEKFQKKFSTPVLFRIAAFFSHTGDSWLWCGIL
                     FLLWIFSSGEQQKVLAFWGLSIAFTAFFIFFLKKVIHRSRPEGEWGMVYRKRDPHSFP
                     SGHAVRAGLILMLAWKTFGMPYFLVFLIWAVLMIHSRVITGVHYLLDIYLGVIFGLLI
                     GCILIGSQDWIYQSFPLLFDRSGWVYIF"
     gene            complement(28555..29571)
                     /locus_tag="PK898_03030"
     CDS             complement(28555..29571)
                     /locus_tag="PK898_03030"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOU19039.1"
                     /translation="MKNVKIGVVGYGNVGKGFVQALNQKTDFYRKIYQLNFFITAIVT
                     KNNGCVYNPNGISLDELLTVNSFAELSDVERPDWNTISMIQQAETDVIVEISSTNLIN
                     GEPAVLHIREAILSGKNVVTSNKGPIALHYKMLNSLANQNNVKLGIEGTVMSGTPSLR
                     FAKDLLFPSGITSVRGILNGTCNFILTEMEKGLSFNEALAHAQEFGYAETDPSGDIEG
                     LDTAGKVAILSQLIFDEKQTPAEMGYEGITGITVEDIQMAKKENCRWKLIGSVKKVQN
                     HIITSVKPEMVPLSNPLANVNGSMNAIQYETDLLGMVTLIGPGAGRMETAAALLEDLI
                     TIYK"
     gene            complement(29586..30647)
                     /gene="asd"
                     /locus_tag="PK898_03035"
     CDS             complement(29586..30647)
                     /gene="asd"
                     /locus_tag="PK898_03035"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU19040.1"
                     /translation="MSNKIPVSVLAATGSVGQRFISLLDQHPWFEVVEITASDRSVGK
                     TYEESCHWLLSDPMPEYVKKMELLPTDADAIKKSQIAFSALPAEIAMIVEPDFARKGI
                     YVSSNASAYRKDPLVPILVPEVNPDHLQILDVQQKTYQWPGAIMTNPNCTTVGFVVPL
                     NAIDKHFCVTKAIVFSMQALSGAGYPGVPGMDILDNVIPFISGEEEKLEYEPAKIMGN
                     FDGEKIVQHPIVISAHTNRVAVTDAHTICLSFAVREKVSEEQIISTLENFEFPEICRS
                     LPSCPKKGIIYHKEENRPQPRWDRSLEKGMATNVGRVRKDNILDYRMVTVSHNTIRGA
                     AGGSILNGELLAKIKFRMN"
     gene            complement(30670..32097)
                     /locus_tag="PK898_03040"
     CDS             complement(30670..32097)
                     /locus_tag="PK898_03040"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU19041.1"
                     /translation="MENTSIPNHILVMKYGGSSVGSPVAMKSAVKIICETKKEYPNLV
                     VVTSAISGATDALIKSATDASSGNLTTAEKTAHELTARHHELIDKLVEDPKIWLQLKM
                     DVNRLISEFVNLCNAIKILGELSPRALDAVSSLGERLSVRILSAAVESAGIKSKPVES
                     TQLIITDDVYQSASPDMELTSVRCKKVLYPILNEGITPIVTGFIGATKEGSITTLGRG
                     GSDYSASIIAVGLEAEEVWIWTDVTGVMSADPRIVPDAKTIPALTYNEVSEMAYYGAK
                     VLHPKSVKPCVENGIKMRICNTFAPQSQGTILIHNNDAVDVGKIKAVTTASGFKLITV
                     SGAGMDSGMNASAKIFNGFVEAGVNVPMVIESSSEQSLCFPIDIKKVNKVEKVLRTIL
                     EKEISRKDIDEFEISEPVDILTVICSGLKSHPEVITQVLSKLIENQYKIKALSYGASD
                     VSLNIIVSSSDSISALKAIHQLISF"
     gene            32389..32817
                     /locus_tag="PK898_03045"
     CDS             32389..32817
                     /locus_tag="PK898_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19042.1"
                     /translation="MIPVLNAAKINCDSILPSRKHSDDAGLDLYASETKIIEGNGINV
                     IGTGIAVDIPKGYVGLIFLKSGSNFLLGGGVVDAGYQGEIKIKIFNIKTETIQIEKGQ
                     AIAQLLIVPIETPEVREVQISDLFKEKSDRGAEGGIHLPI"
     gene            complement(32818..33276)
                     /gene="ndk"
                     /locus_tag="PK898_03050"
     CDS             complement(32818..33276)
                     /gene="ndk"
                     /locus_tag="PK898_03050"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU19043.1"
                     /translation="MEKTFVCIKPDGVQRGLIGEIIQRFENRGLILCAAKFMIVSKEF
                     AERHYAEHIGKPFYNGLVNYITSGPVFAMVWEGEEAVKAVRQTVGATKPIESAPGTIR
                     HDFSAKTDRNLIHASDSVTTAEREINLWFEPDEIVTWDKISSPWVFGNNS"
     gene            complement(33279..34691)
                     /locus_tag="PK898_03055"
     CDS             complement(33279..34691)
                     /locus_tag="PK898_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19044.1"
                     /translation="MAKIELNDYLGRIDTLVSESKYEQAAYHCFYLLRQFPKLLSLYK
                     LLGRIFLEMNELRPALDTYSRIVSAEPDNFLNHLALGFIFEELKLPDQAIFFSQAALF
                     LQPENKEVVSLYQRLLSRMKNYPNPESFSHLLAGIHFYNERNYEKAINELQINSLESY
                     QTFGSLFIGLSLFENHQEDEGIKLLQIILEKSPYLQSALQKTAIYWQKKNPQLFMKYL
                     NRLIELNPDYSEYTIQDNQLTCGNNSIQVIYQEWTGFQNSKLRTVWQQPGSHIIENDL
                     SALPSWLKLLPVCPDFLYQTNNRNEEENVFYSSERKKILLEEDSFFQRDYFKSQIPES
                     DKNSQKQRESTEKDQKIGKSSRSTKSLDDAFHWLEKVVTEGVEIKQEGTAEKQFDSDR
                     KADIDEFENDLQNKEETEMVRNAWTCFSTGNQVEGIRLYKQLIEKQIKPEMVQEDLQK
                     LLILFPENEELQKIINERGK"
     gene            complement(34784..36001)
                     /locus_tag="PK898_03060"
     CDS             complement(34784..36001)
                     /locus_tag="PK898_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU19045.1"
                     /translation="MKEDLLNIDVDEEDDDENYPAIARLIELGRQKSYVTIDDILALF
                     PDAEQDVEQLEEAFSALLSAGIPYSEESNLEEPPEEEIDLAEEENEIIPNISMDDLAN
                     IDTDDTIGLYLKEVSRVPLLTADEEVELAQRIERGRMAREELARVNVTPERRLELRKS
                     IEDGWAAREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRATKKFDYRRGHK
                     FSTYATWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRVQHQLTQKLGRDPSVEELA
                     DALDVPPKKVENMIQVARRPLSLETPTDDEEDSVLGDFIEDEDAPAPDDTATYNLLKE
                     HLDEVLNGLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERVRQIEAQALSRLRH
                     PTVRKILRDYLGD"
     gene            complement(36235..37083)
                     /locus_tag="PK898_03065"
     CDS             complement(36235..37083)
                     /locus_tag="PK898_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU19046.1"
                     /translation="MTRIIGDTTSGLTPEQAKEIGIDYIPQIVIFGDKSYRDDYEIDP
                     VTFVKMLEASKELPKTAAPPPSLYTPIFEKLRENKEDALVLAPSVKVSGTYRSAMTAK
                     NDFPDVDIRVIDTGVVGGPVAMELIEAKKMADQHRSLDEIENYINLLNKNVRFYAVVD
                     TLEYLYKGGRIGGASKLFGDALQIKPILTLKDGQVEAFEKQRTQKKALLRIVDLVQAE
                     CPKGGNHGLISLSLNVNLCQEELSFFRKEFKERFGFENVPVFNAPPAFMVHAGPRVVL
                     VSFFTI"
     gene            complement(37085..38095)
                     /locus_tag="PK898_03070"
     CDS             complement(37085..38095)
                     /locus_tag="PK898_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOU19047.1"
                     /translation="MKFEPINKIPGFKIGHAQDSEALTGCTVILCDEGCVGGVDQRGG
                     SPGTRETDLLRPLHMVETVNAILLTGGSAFGLDAAGGVMKYLEEHHQGVNVGPTVVPI
                     VPTAVLMDLTIGSYSIRPDQAMGYQACLNASADSPKQGNYGAGTGGTVGKILGMDHCM
                     KGGIGHAACELEPGIWVGAIVAVNSLGDVVDPNNRNILAGAQLVDESAADKNNKILFA
                     DTLSILSGKTNQKSLETVPVNNTVIGCVLTNVKLSKDEANKLAQCGQNGLVLSIQPAH
                     TMFDGDTMFAVSSQRKNGNIHAVTAFAPIVVAQAIVNAIRFADSVGGIPAARDFLSTE
                     RE"
     gene            38202..38879
                     /locus_tag="PK898_03075"
     CDS             38202..38879
                     /locus_tag="PK898_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU19048.1"
                     /translation="MKYSKINFEKNNMGFYFVKNVQKSLEKNVSKQFILLIITAIYIL
                     INNNSVQAQIPTLSIPTVTGTDQGVVVTVKNDPANPQINLRAGPGTEYDKVGTMVIGQ
                     KAVAKGRTEGGTWLLIEYPGAPGGYAWVYSTYVDYLGDLPIVTIPNTPTPRVTNTIDP
                     TLAAQFIITVEPTRLPTFTQPAPLAIPTFPQSSSTYESSVPMGLIIVSCAILGIIIGL
                     FTLAQKR"
     gene            38909..39784
                     /gene="ricT"
                     /locus_tag="PK898_03080"
     CDS             38909..39784
                     /gene="ricT"
                     /locus_tag="PK898_03080"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOU19049.1"
                     /translation="MAGNSNPVVVGIRFGKLSKIYNFDATGLDEIHLKDLVVVETCKG
                     KQIGEVVKIYQDYEMKDEPLKKVERIATAGELVSNEFWNLRKKEVLEYCEKRAAELNL
                     KDIRILNAEFNYDGSYMILTYSSPTEEKVDLKSLKYDIQKSFSINNIEIKQIGPRDVA
                     KVIGGMGACGIPCRCCSSFLTDFSSISIKMAKEQGISLTPSEITGMCGRLRCCLEYEN
                     EYYAECRKNLPKKKKKVITPQGEGKVVEIFPLRHSVLVEIPCVGRREFTADQIKYDEI
                     PAKQDDSSKEEEEAK"
     gene            39781..40620
                     /locus_tag="PK898_03085"
     CDS             39781..40620
                     /locus_tag="PK898_03085"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOU19050.1"
                     /translation="MNSMKIGCHLSVAQGYLHMGKEAISIDANTFQFFTRNPRGWKTK
                     ELNIQDMEDLTSLMKSNDFSPILAHAPYTLNPCAADERVHEFALMVMKEDLAIMEHMP
                     ENYYNFHPGSHVGQGIEAGIQLIIEALDHMLYMEQKTTVLLETMSGKGSEVGSTFEEL
                     QEIIKRVKLNDKLGVCLDSCHIYSAGYNIVEDLDGVLEKFDKVIGLNRLKAIHLNDSM
                     TPFGSKKDRHAKIGQGTIGLDAIIRFINHPLLKNLPFYLETPNDVPGYAEEIKILRQA
                     YNG"
     gene            complement(40688..41863)
                     /locus_tag="PK898_03090"
     CDS             complement(40688..41863)
                     /locus_tag="PK898_03090"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU19051.1"
                     /translation="MFNKKTIRDIDVKGKKVLVRVDFNVPIKDGQVGDDTRIRGALPT
                     IQYLLDQGAAVILCSHLGRPKNGPEEKYSLKPVAEYLGTLIPNKVYFASDCIGEPAKK
                     AAENLKPGEVLVLENTRFHPEEEKNDLEMAKQLADLADIYVNDAFGTAHRAHASTEGV
                     THFKPGVAGFLLEKEIQYLSGAVENPKRPFVAILGGAKISDKIGVIKNLLNKADKVLI
                     GGGMANTFFKAQGFDMADSLVEDEAVETAKEILNMGSNKLVLPVDMVIANAFDNNAER
                     KTIPVGNVPAGWRVLDIGPETIKVFSNLIKNAATVVWNGPMGVFEMPNFAKGTFEIAK
                     AVAESNSISIIGGGDSVAAIQESGLADKITHISTGGGASLEMLEGIVLPGVAALQDK"
     gene            complement(42034..43089)
                     /gene="gap"
                     /locus_tag="PK898_03095"
     CDS             complement(42034..43089)
                     /gene="gap"
                     /locus_tag="PK898_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU19052.1"
                     /translation="MKVQVGINGFGRIGRQVLKSFIDNYSDELEVVAINDLFDTKTNA
                     HLFKYDSTYGKFDGSVDVVGNDLVINGKTIKVFAEKDPGALPWKDLGVDIVVESTGIF
                     TDAIGDPEKGKPGANVHITKGGAKKVIISAPAKNEDITIVMGVNDDKYDPAKHHVVSN
                     ASCTTNCLAPVAKIINDAFGIEYGLMTTIHSYTNDQVILDQGHKKEMRRSRAAGLNII
                     PTTTGAAKAVALVIPELKGRFDGYSLRVPTPTVSIVDFVCNISKSTTVEEVNDLFRSM
                     AGKGRLKGILGVTEGTYADPLVSTDFRGDSRSSIVDLMQTSVMGGKMLKIVSWYDNEW
                     GYSNRACDLALMMSKSL"
     gene            complement(43138..44139)
                     /gene="yvcK"
                     /locus_tag="PK898_03100"
     CDS             complement(43138..44139)
                     /gene="yvcK"
                     /locus_tag="PK898_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOU19053.1"
                     /translation="MEDHNQFDKYPKMDHDTKIVVIGGGHGLSTILRGLKRISENITA
                     VVTVSDDGGSSGALRRDLGVLPPGDIRNCLAALSDDEEFLSQVLQYRFSSETSVGGHN
                     LGNLLLTALANITGSFELGIQELTRILAIHGKVTPSTLEDVNLVADVEIENNGKKECI
                     RVFGESNITEKNGTIRNLEIEPKNVIANPEAIKAILAADLIALGPGSLYTSVLPNLRV
                     NGIAQALKASHGKIIYVCNVSTQPGETTNFTCYDHVKVIHNAIPDVSIDGVVCNNYHE
                     AAESKNISWVKIEESILREYRVFQDDLIDKAYPWRHDSNKVAEALLKAYKELGSTEN"
     gene            44359..45192
                     /gene="deoC"
                     /locus_tag="PK898_03105"
     CDS             44359..45192
                     /gene="deoC"
                     /locus_tag="PK898_03105"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOU19054.1"
                     /translation="MNVNSYKYDDEYDQYTDPYNCDLEMIIEEAEGYQVITTYNAEGV
                     IVDVQAHSAEESAEKSSVEKLAEMIDHTLLKADATPDQIMTLCKEARENHFASVCVNS
                     CWTRLCAEILRGSGVKVCSVVGFPLGAMIPEAKAFEAECALENGASEIDMVINIGALK
                     GRDLRLVSKDIQGVVEIAHQSGAIVKVIIETCLLTEEEKIIACLICKTANADFVKTST
                     GFSTGGAKVEDIELMRRVVGDEMGVKAAGGIHTREEAEKMIAAGATRIGASAGVKIIH
                     E"
     gene            45185..46015
                     /locus_tag="PK898_03110"
     CDS             45185..46015
                     /locus_tag="PK898_03110"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU19055.1"
                     /translation="MNESSEKLYYGNIESDGIKLHYARTSLGLRPVVFLHGMTDNGSI
                     WGQLAAALYQKYDIFLPDARGHGLSQGGDIEYSLTAMTDDAANFIRLLELDRPVVIGH
                     SMGASVAGNLAAEYPNLVRAIILIDPTWYEAEYLTAEFARQVISEFEKAYEEYHQLSG
                     IEMIEYAKRHHPNWSESDILPWAKSKQQYDRRIIGMIPTLAENWPQITSRINIPALIM
                     TGDPQKGSIVSQKVSEELLMKHSNWESIYFEDAGHNIQRDQFEKSRDAIVKYLAEIYS
                     "
     gene            46031..46639
                     /locus_tag="PK898_03115"
     CDS             46031..46639
                     /locus_tag="PK898_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOU19056.1"
                     /translation="MCGRFSLMVQLSDLQLEFGLERMPSSFTPSDNISPGSNIPVITN
                     QNPDTLDYFYWGLIPAWAKDISIARNTFNARAETLSEKPSFKNAFKRRRCIIPATGYY
                     EWKKTETGKIPYLFTVKELSIISFAGLWEYWMDANGNEIYSTTIITCPANEYAAAFHD
                     RMPVILKRDQRKIWLGESPESELRLLLEPIHSDELKATPADL"
BASE COUNT        13889 a         9106 c         9642 g        13707 t
ORIGIN      
        1 tcgacaaata tattgtacgg aaaaggttct gtatctgaaa gtaccttaaa aaacatattt
       61 ggttttattc cggaagaaaa taaggtaatt attatatgtt tggtttccga taaaaaagct
      121 gaatcggttc tgaatcttct cattgagaaa tttcatttta atttacctaa cacagggatc
      181 ggtttcacaa ttccgattga aaaaatattt ttttagtgaa acttatgatc attatgctgg
      241 tgcaaatcgg atttgacaga aatcatcaaa aggaacgggt caaatggaac aaaaaagtaa
      301 tattaaagct ctgattatta ttgtcaacgc aggtttttca aatgaagcta ttaaaattgc
      361 gagagaatcc ggagctaccg gcgctacgat attgaatgcc agaggcgaaa gtctgcgtca
      421 tgaagttttt atgggaattt ctgtcgattc cgagaaggaa attattcttt gtttggtaga
      481 agaaatagtt gcagacaaaa tcatgtcgac cattcaagaa aaagcaggta ttaagactcc
      541 cgcacacggt atttgttata cgatgggagt ggaaaaaatc atcgggataa attccttttc
      601 atctcaagcg aattaaatgg caaaaccata aattttatga tgctgttttc gtgttataca
      661 gcagaatact gaatgttgta aaaaccaaat gacgatttgg tataatcgaa atgttatact
      721 gagtaacgaa cgtcgattac tttctgaatg attaaatagc ggaagaaata aaaaagtaat
      781 cgaatttggg aatagaaacg atactggatc tctcggagca ataatttggc attcaatcca
      841 ttaaattggt taatgggctt gttctcatta gatatcggaa ttgatttggg gacggcaaat
      901 acacttgttt acgttcgcgg taaaggtatt gtgattaatg agccatcctg ggttgccgta
      961 gaaaagagga caagacgtcc ggttgctatc ggagcacaag caaaagaaat ggttggtcgc
     1021 acgcctgcaa atttaatggt agtgcgtccg atgcgtgacg gtgtcatttc tgaattcgaa
     1081 ataactcagg cgatgctcag ctattttatt ggaaaagccc atcaacagac gattgcacct
     1141 ttccctcgtc ccagagtggt gattggaatc ccttgcggag cgaccgatgt cgaacgccgt
     1201 gctgtttatg atgcggcgat ggctgccggg gctcgagaaa tatatcttat tgaagaaccg
     1261 aaagccacgg cgatcgggat cggattaccg atcaatgaaa tacgcgggac gatggtcgtg
     1321 gatatcggcg gaggtacaac ggaagtagcg attctttcaa tgaatgagat cgttgtctca
     1381 cgatccctgc gtgttgccgg tgatgaaatc gaccaagata ttttaaacta tatcaggaat
     1441 cgatataatc tttatatcgg cgaaagaatg gctgaacaaa caaaaatttc gatcgcatcc
     1501 gcatatccgc ttccggtaga gcggacgatg gtgattcgcg gcaggaattt ggtaaccggt
     1561 cttccggaag ctatcgaagt atccagcgtt gaaattcggg aagcgatttc cggttcaatc
     1621 caggtaattg tggataccgt aaaagatgcc atcgatgaag cacctcccga attggttgca
     1681 gatttaatcg aaacggggat ttgtttatca ggtggaggat ctcagatttt gggaatggca
     1741 gaccggttaa acgatgaatt aaatattcgc gtctgggtgg cggaagatcc gatgacctgt
     1801 gttgcccgcg gagccggaat ggttctcgaa aattcatcga caatcgaaca atttgccgca
     1861 agcttagatc gatttacccc caagaaataa tttatttgag acttaattat ttttctaatt
     1921 ttctttatcc cttaatgttc tttctgatct ttacctgttc ccaaattgtt ataataattc
     1981 aaagatatga ataatgttaa aaagaactgg cagaagatcg tagcgacctt aattatttcg
     2041 ggagtcatat ttttggcttt aagcggtttc ttgacccaat ctttgagcgg gttaattgat
     2101 ccgattattc aagttcaaaa atggaccgcc gaaaggattc agacggttta tgagttcttt
     2161 acaataccga gagatgttac tcaactccgg gctgaaaatc aagccttaca ggaacaagta
     2221 tctcttttac aaacggaaat tattcaatta caacaagact tgaaagaagc ggatattctg
     2281 tattctctgt taggattcgc gcgcggaaga ccggaagaga catatattgc nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgatcgggc agatattaaa tgtgaataag
     2461 ctggaaaatg aactatttca atcggcatcc attcaaccgg cagaggattt tagtaatctt
     2521 caggcagttt tggtggtggc gaatttccgc ccttcgaatg tggaaccttt ggaatagaga
     2581 tagttgtcga ttcaatgaaa gaaattattt cctcatttgt tatcctgatc ttaggtggaa
     2641 tcctacaagg atccttgttc agtcgaatac atattttgaa cggtaccggt gatgtgatca
     2701 tggtaatgtt gattgtttgg tcactcaatc cgtatacccg ttattcatgg atttggcttc
     2761 ttttaggagc tttgataatg acttatttgt cagctttacc tttatatgga tattttttca
     2821 tttatggaat tatttggctg tttataaggt ttctgaaaag tcgcttttgg caaatgccta
     2881 tcatattaat gatatttgtg acaataacag gttcgattat ttcagctgtc atttcttatt
     2941 ttgtattgta tatcaacgat atttcaccga atttgaaaac caccgtaact caaattgcgg
     3001 tgccgtcact ggtattaaat ctaatctatt gtttcccaat ttatggactg ctgaatgatt
     3061 ttgcgaattc tgtttgttat aaggaagaat cttcataatg aatgaattta atcgagaaaa
     3121 actggatttt aaaaaccgaa gatttgtagc attttatgtg gtgatgggtg ctattatcct
     3181 cttttttatt tatagattgg ttgtcattca actggtagaa ggagactatt atctcagttt
     3241 agccgatgaa aatcgaatat cgattgtaaa ggaacaaact acccgcggaa ttatttatga
     3301 ccgtaacgga gtggtcctgg ccaggaatgt acctacgtat gacattgtta ttacaccggc
     3361 aaatcttccg actgatgaag gagatatcca agccatttat cgaaagctat ccgaaatact
     3421 cgatattcct gtaaataacg gtgtaatcaa tgaagagact gtgcggcttt acagtgaatg
     3481 cgggacggat ttcggaatat ctcaaattat ttatattgct aactcgttga ctccttacgc
     3541 ggagaccgcg attaaatgcg atgtgccgga agaaaccgca atgattgttc aggaattagc
     3601 ggcaaatatg cccggcgtcg gggttcgagt caattcgatt cgagaatatc caacggggta
     3661 tgatacttcc gaggtgatag gatttctcgg tcccataccc gatacggttg ccgacgagat
     3721 ggaaagtcgc ggctttatat taaataagga taaatatggg tatgcgggtg ttgagtcttc
     3781 cttacaggat ttgttatccg ggaagaacgg gcgtcgagtc gttgaaaaag atgttgccgg
     3841 cttggaaact cgggatttgg aaccgccaaa ggatccggtt ccgggctaca atgtgaaact
     3901 gacgattgat gtccgtttgc aaaatgtcgc cagagccgca atgttggata cgatggattt
     3961 ttataatcgg atcagtgtca ccggcccatt aacgcatgac ggggctgcaa ttgccatgaa
     4021 tccgcaaacg ggggaaatac tggctttagt ctcagaaccg actttcgaaa ataatcgaat
     4081 gacccgttat attccggctt attattatca gcaactatcg ttggatcagt ccaaaccgtt
     4141 actgaatcat gcgactcaag tggctcaacc tccgggctcc gtattcaaaa tcgttacagg
     4201 tatcggcgcg atcaatgaga aagtggtttc gcctgatttc catattaatt gtcccggaca
     4261 tatctttatt caaaacagct attctccgaa tgatcccggt tcggaacaag aatatgtatg
     4321 ccatttacgg accggtcatg ggcaggttga ttatttgcat gcgcttgctt ggtcatgtaa
     4381 cttgtatttt aataaagttg gcggcggata taaagatgaa gttcctgaag gattaggaat
     4441 cgaccggctc ggacaatatg ctcatgcatt gggatacgga gaaatttccg gaattgaatt
     4501 aacgggagaa gagaccggta tgattccgac cagaacctgg aaaagggtga ataaagggga
     4561 atcttgggcg acaggtgata cttatttggc ttctatgggg caaggttata tcagcgcttc
     4621 tcctttgcag gttctcggtt cgtttgttac cgtcgctaat aaaggaattc atattaagcc
     4681 cacaattatt aaagaagttt tggatgatcg cggaaatgtt gttcttcctt tcactccgaa
     4741 aatgttatgg gatatcacaa aagaccccaa aattatggtt tatgatgaaa acggagagga
     4801 gaccgggaaa tataaaacgg ttgatccgac agtaattgat ttaacccggc aaggattgcg
     4861 attagtgacc caaccgggag gaaccgccag cgaagctttt gccggtgata cacatcaatc
     4921 ttcggctaag accggaactg cggaatattg cgatgatatt gcgatggcga aggatgcatg
     4981 caaattcgga aactggcctt cgcacgcttg gacggtagct tatgcaccct acgacaaccc
     5041 ggaaatagcg atacttgtat ttgtttataa cggaaaagag ggcgcttata tggcaggata
     5101 tccggtacga agaattttgg ataactattt tcttttgaag gaatatgatg ccgagcgggc
     5161 cggcgaaacg ccgggttctc tgattcaata agatttatgc gtataaaaaa gataaggttt
     5221 gaaaaagatt aagaacgaat gagaaaagag ttttggcgta gttttgactt ttggcttttt
     5281 ggtgatattg tcatcctttg cgttttcggt attgtgatga ttcaatctgc cttagcgggt
     5341 aaccttgagg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgatggta
     5461 atttggttct caatgttgtg ggtgagtaaa attccgacga aataccttat cattctcgga
     5521 accatcggag tgatcttgtt gatcgggata accatctgga ttttggtttc gccggatacc
     5581 atcccattta ttcaggaata tcaattaaac cgactgaaaa atttctttaa tccggatgaa
     5641 aatgccagat acggtgataa atataatgta gaccaagcgg ttatttcgtt aggatccgga
     5701 aaattattcg gccaaggtta cggcaacggt acgcaggttc agttaaggtt tctgaaggtt
     5761 cgtcatacgg actttatttt ttcggtattg ggtgaggaat tcggttttat cggtacaatt
     5821 atcgtaatat ttttgctggt ttttgttatc ttgcgctgtc tacgggcggc aagattggca
     5881 ccggacctat ttggatcatt gatttgctat ggcgtagcca cattgatttt tttccaaatg
     5941 gcggtgaata tcggcgtaaa tcttcaagtc cttcccgtaa ccggattaac attacctttt
     6001 ataagctacg gaggatcatc gacacttgcc ttaacaattg cgatcgggct ggttgaaagc
     6061 gtcgcgaccc gacaagaaaa aagagaagag atcgtgtaaa atcagaatca aaatataaaa
     6121 aataaatttt atagacgaaa agagaaaaat tctaatgaca aatgaaatga aacccgtacg
     6181 tgttcgattt gcaccctccc ctaccggaag actccattta gggggcggaa gaaccgcgtt
     6241 gtataactat cttcttgcga aaaaaaccgg aggaaaattt atcctcagaa ttgaagatac
     6301 tgatcgcaag cgttatatcg aaaatgccga acaggaattg atggatgggc ttcattggtt
     6361 gggaattgac tgggatgaag gacctgacaa accgggtgaa ttcggtcctt atcgacaatc
     6421 ggagcgaaaa gagatttacc ttaaatatgc ggaacaatta atcaagcaag gagacgcttt
     6481 ttattgtttt tgcactcccg agcgtttaca tcggatgaaa gaggaacaag aaaaaaggaa
     6541 agagttccct cattatgacg gacaatgcag agatattcct cttgaagaag cgagaaaaag
     6601 aatcgcggcc ggtgaacctt atgttatccg ttttaagacg ccgaaggagg gtgttacgac
     6661 tgtacatgac gccatccgtg gggagataac agtagaaaac cgaacaatcg atgactatat
     6721 cattgttaaa tcggatggtt gggcattata tcatttagcg gctatggtgg atgatcattt
     6781 gatgggaatt acccatgtta ttcggggttc cgaatggata ccgacacttc ctttgcatca
     6841 tatgattatt caggcattcg gatgggaaga accggtttgg attcatcttt cagtttttct
     6901 gaaacccagc ggaaaaggga aaatgtccaa gcgagaatcc gctgatctga ttaaagacgg
     6961 atattcaatt tttttgaccg attttaaaga tttgggttat ttgcctgagg ccgtcacaaa
     7021 ttggatcgcg ttgatgggat ggagttatga cgatcataca gagtttttta caatggaaga
     7081 cttgatcaag gatttttcga tagaccatct gaatccttcc cccgcagcga tcaattttac
     7141 gaagctcgat tattttaatg gcgtccatat tcgagcatta ccggttgaag atctcgctca
     7201 ccgaattaag ccgttcttcg aggcaaaagg gtatcatccg gatgacgaga cattgttgaa
     7261 aatcacgcct attattcaag aacgaatcgt cactttagat gatgcgcctg agatagcggg
     7321 ttttttcttt gaagatcatg tcactccaca gacagaagat ttaattcaaa aggggcttac
     7381 cgccgaaaaa tgtgctgaaa tagccgagaa aagttatcag gtattggctg atcttccgga
     7441 aatcagtccg cggatcggag aaccggagat gagagcgttg gttgaagaat tagggttaaa
     7501 gccggctcag gtttttgggg tattgcgcgt agccgtgacg ggcagaaaag tcagccctcc
     7561 tctgtttgag agcatggaaa taatcggcaa agacattgtt ttagagagaa tcaaaaccgc
     7621 agcaaagatg ttacgagaat atcaaagccg gaataattaa ccgaaaagaa aggagcagtg
     7681 agaaatgaag aatttcagtt ttcaaatacc cactcaaatt atttttggaa gcggaacatt
     7741 ggaacaactt aatacgattt cgttacccgg gaaaaaagca atgattatta ccggagggac
     7801 ttccgtacag aaatacggat accttgatca ggtttataaa attctaaaat atcgaaatat
     7861 tgaccatgtg ttatacgata aggttgtgcc gaaccctatt cggaaaaatg tgatggaagc
     7921 cagagatatt gcaagagaaa aaaagtgtga tttctttatc ggcttaggcg gaggaagcag
     7981 tatcgatacg gcaaaatcag ttgcgatgat gatgactaat ccgggagatt tatgggatta
     8041 tgtaccggtc ggacaagggg gcaaaaaaga ttttactcaa aaaggattcc cattaatagc
     8101 cattccgact acagccggaa ccggaaccga aggaaacccg acgtctgtaa ttaccaacga
     8161 aataaccggt gaaaaagtcg gaatacgctg tgagtttccg ataatatcca tcgttgatcc
     8221 cgatttgacg ataaatatta atccgcgtta tacaacatat cagggatttg atgcattgtt
     8281 tcataatata gaggctttta tttctcaaaa agcaaatcct ttcagtgatc ttcttgcact
     8341 tgaaggagtt cgcttaattt ttgaaaattt acccgaggct gttcgaaacg gaaagaacat
     8401 cgaagccaga tctgcggtct cttgggcggc gactcttgga ggaatggtac tttgtttagc
     8461 ttccgccacc tcagctcatg tgatcgaaca cagtttgagc gggattgatc cgactgtcat
     8521 tcacggagag gggttgatca tgatctcaaa agcttttcat cagttaggag cggagagaat
     8581 accggaactt tattcaaaat tagcttttgc gatcggtatg ggcgattcaa aaaagaccgt
     8641 tcaagaaaat gccaaagctt tcttggatgc acttgaagca ttaaaaatcg catgcggcgt
     8701 aggaggtttg aacttatctg accatggatt taccgaggat gatattgatc gtatagccga
     8761 gggagcttat gttatcggag gtggcgcttt aaccagagat caatacaaaa tttcagatca
     8821 agatttaaaa gaaattttgc ggaattctat gcatgactga aagtgttttg attaccggat
     8881 ccgttcgaag acttggtaaa ataattgcct gtcatttggc tgatttgggt tatcatgtct
     8941 ggctgcaata tttacatgat gacatggaag cagagaacac ctgtcaacaa atccatcgat
     9001 ttggaggggc tgcttcactt ataaaagcgg atcttcaaaa tgtcgatgaa attatacgaa
     9061 tgtttgattt tatccgtgaa aatggcgggt tgacaacact cattaacaat gccgcaattt
     9121 tttatcgaag caatttagcc acattatccg taacagattg ggaccgttta ttcgatatta
     9181 atttgagagc cgtttggtta tgttccagag aggcggcgaa attgattggt gaaaacccga
     9241 cgaaatcggg tttgattatc aatatgtccg attcgggatc ttaccaatat tggaaagaat
     9301 atggcggata tactctgagt aaagctgccg tacgtgactt aaccattctt ctcgcaaaaa
     9361 cgtttgcgcc taaaatccga gtcaattcaa tttctcccgg tctgattttg cctgaggaat
     9421 cgatggatga gaagacatgg aaacatttat cctctgagac tttattgaaa aaatcagggg
     9481 aaccggaaga tattttaaac gctgtcgatt acttgatgaa atcggattat ataacaggta
     9541 tcgatattcc ggttgatggc ggatatcgct ggaaatagat gatgcaatcg aaattttacc
     9601 gacaattgca aatgatctta aaggtcgttc cttggtggaa gataccgatt agtttagggg
     9661 tgatgctttc aaccgttgaa tttattaatt tttgggatgt accgataaat tggacggctt
     9721 ttgttttgat ttttttaata acgattattt ctttaatcac agccaatctt ctccaaatgc
     9781 ggctttatat tttttcatcc gatgaaaaat caattaggtt cttaaatgta ccttggggaa
     9841 aaccgattga gatcaataag aatgaaaaag atgccgtatc ggttaacctg atcatatatt
     9901 tcttattctt tttattagtt gctgtatata tgtcttttct tttcttgaat aaaaagaatc
     9961 aatggaactt aaactttgtt ttcctgttga tgattatttc gattttaatt ttctcttttg
    10021 cgggaaacag cccaagaatc aactggtcgg aagagctttt agacggattg ttaactgttt
    10081 tcttcccatc tgtcttagtg atattgggcc atcaaaatta tccttttcaa aaatatattc
    10141 tcagctatct gattccggat ttttgctttt ttctggcatt ccgattcata ttgggtatgt
    10201 caatgctaga ttatcaagtt tcctttaaac caggcttttt tgtcaattat ttgcagaaga
    10261 gaaaattcag gctgcatcat ttattttatt ttttagcata cttttcactg attctaatga
    10321 atcggcttgg tgtaagttgg gagttattgg cgccgcaatt acttctactt ccatcagttt
    10381 ccttccagat tgttctgatt gagaaaatta tgaagggttc tcccgtaaat tggaaattat
    10441 tagggtggct ctcttatcta aatcttattc tgttgctata ctttcaatgt attacgatcg
    10501 ttttttatgg attctaatta aaaagaaatt gatcaataaa attttgtata gcggattata
    10561 aaatgtcgat aacaacactt cgataaaaag gtggaattat gaaaatcaga tcctgcaaat
    10621 atgtaatttt agctttatta gctttttttg ttacatcctg taacatgcct gagcggaatg
    10681 tcgataaaaa tgtgctttat accattgccg catcaaccat tcaagccgaa aataacagga
    10741 tcgcttctct tacaccgccg accccaacct cgactttgac agaaaccccg acgattacgt
    10801 ttacgccgac gataacatcg actccgacaa tcccgccgac cgcaacttgg attactcatc
    10861 cggccggatc tgcagaagtc ctgatccttt actataatga tgttgccaac aatgcgaatg
    10921 aagaccctta ttatcaatgg gaaagtccat taaatgttcc ttccgttgaa tttgaacaac
    10981 aggttcggat attgtatgaa ctgggatata caagtatcgg gctgtcgcag ttggtcaaag
    11041 tattgcttga aggaggggaa cttcctccga agccggttat gttcactttt gattcatcca
    11101 aactcggaca atataaaaac gcttacccca ttctcaaaaa atatgggatg gcggggaatc
    11161 tgatgttagt cgctgcacag gttgatacga agaactgcct ttcatctgag caaataaaag
    11221 aaatgatgaa tgatgggtgg gaacttggca gcagcggata tgacggaaat catgatcccg
    11281 cttattacgg gcaggaaatc ggtaagtcca aaatattgct cgaagaaaaa ttcggtgtgc
    11341 ccattgaagt ttttgcttat ccgggaggaa ttccagatgc cggcggtgtt atggccagta
    11401 aagtcagttc aagtttatat aaagcagcaa tgggaaagca acatgtaacc aaacaaagtc
    11461 gcgacaatct ttattatctc ggccgatttg aaataaccaa agggctgcca ttaaatgatt
    11521 ttctgagtta cctcccttgg aaagagggaa atatttccga agagacaatg aattggacgc
    11581 tgccgacggt tactttcaca ccgcctgctg ccttactgca aacccaggaa accccggctg
    11641 ctccgtaatc ttaaacactt ctcattttta aaataaaaag cgctccattt atgaaatcgg
    11701 agcgctttta tttttaaaaa taccgaattt tttacgtcaa attaatccga ttcggatctg
    11761 aatcaatcat tcgggatctt atgccatatg atcagatatt taggattcga caagatcgga
    11821 gagcttatat tccttcaata tattattgat ttttgtgccg atttccttcc ataaacccat
    11881 tgcaacgtca ttttcagatg tatcgatatc ggtttcaatg gtcggtacag agaattgccc
    11941 ttccaaggca ttaaagatat cataaagtgt gatatccaaa ccttttgaac ccaatcttaa
    12001 accacctttc ggacccggcg tcgctttgat cagaccattt tgctgaagtg tatgcgcaat
    12061 ttggtgcaga aatggcaacg gaatggaaag actttccgaa attgccgccg tcgacttgga
    12121 cgtattattc ggatccgaag ctaacgcaag cattagctgt aacccataat ttaatcttcg
    12181 actcaccttt aacattttta cctcccaaaa aataagtatt gatatataaa tggataattg
    12241 ctatatattc ttcttttata tcatatatta ccaacaaata aggaaattac tttccttaat
    12301 gtgagtgata atgacttttt aaaatattga aatattatat aatgaaaata agaaattatt
    12361 gcagcttaag gagaatttta gattcattcc ataattcttc aaggctgtaa taatttcgtc
    12421 tgtccggtga aaaaatatgg acaacgatat ctccaatatc aagaagcatc catccccctt
    12481 ctgcgatgcc ttgcacgatg caatgaactt gatatttttc tctgatttcg ttttttaccg
    12541 catcggacaa gctttgaagc attctgtcac ttgtacctgt gcaaatgata aagtaatctg
    12601 taaaatcgac ttgttctctt acatccatca gcgttatttc ttccgcttta tgattttcca
    12661 aaatttcgac aatttttcta gcaaatgtta cggtttccag tttatcctcc tctttatttt
    12721 atagttttca tcatcttaac atactttgag attttgtgta ctgattacct gatattcagg
    12781 ttttccggga agcaatatac tcttcatcag agagattttg tcgacggtaa aaggcggaag
    12841 atcgattttc tcgagatttt taataatttc atctttttcg acaggaatca ttgtagatac
    12901 ataatccgga attcgagcca acgttatgtg cggttgaaat ggttttaaat ctttcgaata
    12961 tccgattttt tcaaactcat tctcaaggtt gttcaccaaa acggataatt ctttcgaaat
    13021 atcaaatccg gcccaaagaa tacgcggctt ttttatcgtc gggaaaacgc ctccggaatg
    13081 aattttgagt tcgaaaggac cgattccgcc gattgaattc gcaatcgctg tctgaagatc
    13141 accccttttg tcttgttgta catctcctaa aaacttcaat gtgatatgaa ggttctcttt
    13201 ggaggttaat cgtgctttct tttttttgat aatgacagag tccttcaagc tgtctgagag
    13261 tggttttcga atcatctcag gaagctctat agcaataaaa agacgaagag tcgcattttg
    13321 ttttatcatg attttataaa cagcctcgaa ctaaactgaa tgcaaattat accttttttc
    13381 catactgata ttaaatgaat attgcaagaa taaagagtaa ggaagggaga aattcaaccg
    13441 aaaaaattgt ctgtttaatc gctcttttta ttcactatcg atatctgctg gtttataata
    13501 attcagcaaa aaagatgaat aacaaacagg attaccggag agtctcttat ggaaaaaaag
    13561 cttagcggga atgaaattcg gcaacaattt attgattact ttgttgaaag aggtcataca
    13621 gcggtacggt ccgcatcgct tgttccggct gaggatcaga ctcttctgtt tacgaatgcg
    13681 gggatggttc aatttaaaga tgtttttctc ggaacggacg taagacccta taagcgtgct
    13741 tgtgacagtc aaaagtgtat gcgagtctcg ggaaaacaca acgatttgga tgatgtcgga
    13801 cgtgatgatt ttcatcacac tttttttgaa atgctgggga actggtcttt cggagattat
    13861 tacaaaaaag aagcgattac gtgggcatgg gatcttttga ccaatgtatg gggattagac
    13921 aaagatattc tttgggctac atgtttcaga gatgaaaaag atgagattcc caccgatgaa
    13981 gaagcctttg aaatttggaa tactcagccc ggaatgaata aggaccacgt cctgtatttc
    14041 gggcggaagg aaaatttctg ggaaatggct gacacgggac catgtgggcc ttgcagtgag
    14101 attcacatcg atttgggtga aagccactgc aataaaaaag gcatcccggg tcatatttgt
    14161 catgtaaacg gagattgcgg tcgtttcttg gaattatgga atttggtgtt tatccaatat
    14221 aaccgcctga atgaaactac attaaatcca ctagcccagc atttcgttga tacgggtatg
    14281 ggatttgaaa gaattgtatc gattctgcaa aaaacagact caaattataa gacggatcta
    14341 tttttgccga tgattcgtga aattcaaaaa ttaaccaaag taagcgatga cgagatgtat
    14401 tcgaatttta cgccttatcg agtgatcgct gatcacgctc gcgcctctgc ttttctgatt
    14461 gcggacggtg ttgttccagg aaatattggc agaaactatg tttgccggat gattattcgc
    14521 agagctgttc gatttggtta ccaactcggc ttgaaagaac cgttcatgag caaagttgcg
    14581 gaaaaggtga ttgagaatta tggagatgct tatccggaat taaatagaat gaaagaatca
    14641 attcttcatt cggtatcctg ggaggaagag agatttaata aaacacttga aaacggaatc
    14701 aaccaactca atgaaattat tgaggaaatg aagaagggcg gtcaaaatat catcgacggg
    14761 gaaacttgtt tcaatcttta ttcaacgcac ggtcttcctc tcgaaattac ctatgatata
    14821 gtccgtgaat acggtttgga tgttgatcgt aaaggattta aaaaagcaag tgaagctcat
    14881 cgcattgctt ccggtgccgg gaagattttt ggaaaaatgg ggggagagga tgcggaacaa
    14941 tatgggatgc atctgtcctt tttgaaagag tcaaagttgt tgggtgaaga aggcgtaatt
    15001 tgcgatcctt atcagaccaa ggatgtagaa gccaaattga tcatggctct ggtagacgga
    15061 cagcgtgttg aaaaaataga agaaggtcaa tttggcgaac tgattgtttc gcagaccaat
    15121 ttttatatgg aaatgggcgg ccaagtggga gacaatgggt ggattcgttc ggaagacggg
    15181 tcttttgaat ttaaggtaga aggaatccgt aggccggttg ccggacttac cgttcattac
    15241 gggaaagcag aatcgggaag tatttcggta ggagagactg tcaaggtggt tgtcgataan
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcactcatgt aagcaatacc
    15421 ggtgaaatcg gattgttcat gataatgagc gaaggcagtg tttcgacagg catccgacgt
    15481 attgaagcta ttaccggacg cactgcaaat atgactgcac gaaagaatac acaaatagtg
    15541 aagaatttgg catcaatctt aaatgcctcc acagaagaaa tattcgaaaa agtggaaaat
    15601 ttgcaaacac tttcgaataa gtatcaaaaa gaaatcgttc aacttcaaac acaaattgca
    15661 ttaaaagatt ttaagaagtt ttgggaaacc gcacaaaaaa ttaaagatgc atatgtatta
    15721 actgcaatga ttccagattc aacaattgaa tcgctgagaa atatggccga tcaatttaaa
    15781 gcagaaaatc caaatgctgt tgtcgtctta ggcacgaaaa cagatgacgg acaagttcaa
    15841 tttattgccg ctgtttctga ttcactgatt caacgcggaa tcaatgccgg ggagatcatt
    15901 cgaaatattt cagctcttac cggaggtaaa ggcggaggac gtcccaatat ggcacaggga
    15961 ggcgggaagg atatcaacaa agcgcaggaa gcgttggatg ccgctgtaaa aatggttcgg
    16021 gaaaagcttc gataaattaa tttgtgaaga aaaattgtta atcggcaagg ataccgaaga
    16081 aactttattc aaccgaataa taaaacattt aatataaaaa tgagggcgct atcaaacttt
    16141 tatggtgccc tcattcttgt taatcttaat ttgtttattt tttttactta tcgatgattt
    16201 cccaaaaaga ataaagctaa gacaccgggt cctgtatgcg aaccgataac attgccgaca
    16261 taattaacgg taaaatcatt gacccccaaa ttatcatgaa ttaatttctc aagtttttcc
    16321 acatcttcca tacaatcacc atggctgatg aaaatccgtt ggttctgagg ttctaccgcc
    16381 atttctttca ttttttcata taatcgtttg atcgctttgt ttcttcccat aatcttttct
    16441 ctgggaatca aatgcccggc atcatccatg ttcataaccg gtttaatatt taatgcactg
    16501 ccaaaccaag cggagctggc tgatactcta cctccgcgct ccaaaaaatg aagatcatca
    16561 acggtaaacc aatgattcat tttgagttta ttttcttcga gccaatccct tacctgttca
    16621 atactttttc ccgaatcccg ttgtctgatc gcataatcga cgagcaagcc atatcccatc
    16681 gaagcagata aagtgtcaac ggtaaagatt ttgcaatccg gatatttctt cgaaagggtg
    16741 tccgccgcca tttttccgct tccataggtc ccgctcagtc ccgaagaaaa accgatatag
    16801 agtatttctt tttgtccttc cttgattaat cgctcatatt cttcgataaa ggcttcggga
    16861 ttaatttggc tggttacgga catacttccg tttctgagcc ttctgtaaaa ttcttctccg
    16921 ggcatatcaa tcggtccggg ctctccgtct ataatatatg agagatacaa caggctgaag
    16981 ttttgataag aattcgtcag gctttttggg agatcgctac ctgaatctac aagaatatgg
    17041 aatggcattc atcctcctgc aattgtttgg aaagctatat agtgacaatc ttgttgatta
    17101 taaccccaat gagaagcatc aacggataat caggaattat tctacgttaa atatatttta
    17161 aaaatatcta tttagaaata ataaagagga ggaggttaaa ataaaactta aatatcagaa
    17221 aatgaaaatt ttaaatggta aaaatatata tgaatagaaa tatcaaagga aaagtatgag
    17281 gagaccatgt taaaagtcgg ttatatcggt ttgggaataa tgggaaaatc catggcacgg
    17341 aatattttgc atggcggtta cccggtcatg gtacacaatc gcagcagaga gatcgtaaaa
    17401 gaattggtat ccgaaggggc aacggaagct ttttctccgg ctgaagttgc cgcaaataca
    17461 gatgttgttt ttacgaattt accggattcg ccggatgtcc tagatgtggt ttttggggaa
    17521 agaggaatca gtgagggagg aaaaccgggt ttgatttatg ttgatcattc aacaataaaa
    17581 ccggctgtat cgcgtcaaat atatgaaaaa ttagcggaaa aagggatcaa atcattggat
    17641 gcaccggtca gtggcggcga tatcggtgcg aaacagggta ccttgacaat tatggtgggc
    17701 ggtgatgatt ttatattgga aaaagttaaa ccgattctga gtttatgtgg taagacaata
    17761 acttatgtcg gtggtcccgg tacggggcaa attgccaaag ctgcaaatca aataatggtt
    17821 gcggctcaaa tggtcgcaat ggcggagtta ttgatttttt cacaaaaagc gggggctgat
    17881 tgtaaaaaag tggtcgaagc aatcaaagcg ggggcggctc aatgttggac tttggatgta
    17941 aagccacagc ggttgtttga agggaatcgc gaacccgggt tcaaggctta tctgcaggca
    18001 aaagatttaa atattattat ggaaacggct cgtgagtacg gaattccatt accatctgcg
    18061 gcgatcgatg ctcaattgta taatgcgatg cttcagttgg gaaacggaca gcaagataat
    18121 tcggcgatta tttctgttat agaacaatta gcaggcgttc atttaaatga atcgaatcat
    18181 taagacgggg agttatgaat aaaagaaagt ctattgggtt attgtttgtg ttattgttga
    18241 tttgtcaact atttccggat tttaaagttt ttgcggattc ggattcatca tccgagattt
    18301 atcaatccga aactccttat aatatggttt ttatcgatgc gggctattcg acagtaggag
    18361 cccaacagca agatttaaat gcgaaagaag atgcaaaacc tttgcatatc gtttatttag
    18421 atccttatta tttcgatata ttagaggtta cgaacgggca atatgctgaa tgtgttgcgg
    18481 ccggtgcctg taaagaaccg gaagtaaaat caagcgaaac acgagatccg tactatggcg
    18541 tcgactggtt ttcacgtttt ccggtgatta atgtgacttg gcaagatgca tcggattatt
    18601 gcgaatatgt ggggaagcga ttacccactg aagcggaatg ggaaaaggca gccatgggag
    18661 cggaagatta ccggcggtat ccatggggaa attcagatcc caaaccctat acaatcaaca
    18721 tgacgaaagt gccgggcgat acagaaatgg ggaattctta tccgaaaggt gccagtcctt
    18781 acggtgtagt tgacatggtc gggaacgtat ctgaatggac ttcagattgg tacagtgcag
    18841 attattattc tgtttctcct gaaaaaaacc caaccggacc ggaagaaggg accgaaaaag
    18901 tcgttcgagg agattctttt aagaccgata ttcattcaat tcatattacc aatcgatatg
    18961 caatggatcc caaaacagca aacaattata cgggatttcg atgtgcaaag gatgttcgag
    19021 agttagtgga atatacgatt accccccaac ctgaagaaac aataaccaca ataaaaaaag
    19081 cgattgtcaa ttcgggacaa gatgaaggga tttttgtatt ggaagaaccc ggtgttggga
    19141 agaaattaaa ttgtatcgct aaaaacggat ctgttgttga agtatcggaa ggtccaaaag
    19201 aaatggcgta tacacaatgg tataagattc gaacacaatc ggggtgcgaa ggttggtcac
    19261 ttgccaccag cttgaattta caataaaaag gattaactaa aaatctttat tatttccgat
    19321 gagattttat ttaccgcttc gctgataagc gggtggaccg gatcgagata agataagttt
    19381 tttggttgga tcaagagcaa tcctatttca caatccagtt ctgccgcaag gaaaccggaa
    19441 aataatgaaa gcggaagggc atgagtcgaa atctcagatc cttccgcttt tttccaactg
    19501 aaaaagccga tttccccggc atcttctcca aagtcgcccg catcaatgat cagaatataa
    19561 tccggagaaa tctttctgat tacaccggtg aagttttccg gaaccgggcc gcaatcgaaa
    19621 accgagatgg aaaaattgcc cgtttgctgc ggtaaagaag cttgtattga cttggcggct
    19681 aatgacccgg caaaatcatc tcctttgact tcgtttccta ttccgagaat cacaacatta
    19741 ggattgtttt tcttcgctaa cagctgcttg cgcaccggga ttagttgttt ctgccaagat
    19801 tcggtcaata tattccttct ttccgtaata catttggaaa ccgcctttcg atccggctgc
    19861 cgactcccac attttattgg tcatatgaag cgcgtcaaat ttgcagttat aaacacattg
    19921 tttacagtat acgcattctc cgatgttata ggtcataaca aattgttttg ttttacgatc
    19981 aacaacaata atcttgatgg tacgagtcgg gcaatcgcgt acgcattgca tacatcccgt
    20041 acaattggaa gcggtataag ctaattttgc tcgatatctt tccggtggat ccgttttcac
    20101 atacggatat tttgtcgttg ccggcctctt aaacaatgct gaaacaacat cagatagcat
    20161 tccaattaat ttcatagtat tctcaccaga atcaatatca taagttggat aagtgataag
    20221 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgatcat ccaggtatgg
    20341 gctgaagcgt attctgaaat cacattgata ttccaagatt gagccgaaat ggcaggcatc
    20401 aagagcgcca gcatgaacgg tgcttcataa gcaaataact gtgtaaatgt tctggtcgcg
    20461 ccgacgactg caaatcgatt cattgagttt gcaccggcca tccctaaaca catggtcatg
    20521 accgacaaaa gatacattgt gataattaaa tctccatgca aaccgatcgg tgcttcgact
    20581 ccgaacatgg ggacgtaaag tgatgcggtc atgacacatg cgaaagagat aatcggcaag
    20641 acaatgaaca atgatctgtt tacggatttg gggattacct cttcttttac cagtaatttt
    20701 aacatatcgg caaccggttg aaatactctc gggccgactc gattttggaa tcttgcgact
    20761 aattttctat cagcccattc gtataataaa gcggccaata tcagaaataa tccgccagga
    20821 aaaaagaaaa gacaaataag aggatgtggt gcgctcattt gtagtatgct atcccatatt
    20881 ttcgtaattg ttcccaggtc caaatttccg gattccctga gcctttatta atataaacca
    20941 ttcggtcgtt acaggaaaag caaggatcga ttcctgcaag gatcatcggc atatcggcca
    21001 gtttatgacc ttttgccacc gaagaaacga tcgttcccca attacataga ctcgctgtac
    21061 gaatttttac ccgatccgga ctttctgtgc cgtttgatcg gacgaaataa aataactcgc
    21121 ctcgcggagc ttcaaccgat gccatatatt caccggtcgg aatctttcga ggaattttca
    21181 cgctcagttc accttccggc atatgattca tcacattctt gatgtagtga atggatgcga
    21241 tacattcttt tattcttact acaaatcgag cgtttaaatc tccccggtta tctaaagtta
    21301 tatcgatggg aaataaatca taaccatcat aaggacgatc gatgcggata tctcgagcta
    21361 ctccggatgc tcttgcggtc ggtcccgtcg cacccaaacg atcggattct tccaagccga
    21421 ttgttccgat atccactgtc cgttgtcgaa atgtcatatc tgtattcacg acgtcgagat
    21481 aatggtacat tcttttttca aggtaatcta atttcggagt tactccgtca ataaattcct
    21541 gtgtgatatc ttctttgact cctccgggga tatttaccga gtaatttaca cggttcccaa
    21601 aaaagttttc caaaatatcc atcacgattt ctcgatctcg ccatgaatac ataaacaacg
    21661 catcaaatcc accctcatga gcggcaacgc ccagccataa cagatgacta tggagacgtt
    21721 caatctcccc tgctatgcag cgcaccgctt tcgctcgcgg cggcagctcg acacctgcga
    21781 gtttctccac acattgaata aaagcggtgc cgtgggaatg cgaacaaata ccgcaaacgc
    21841 gttctaacag atataaatcc tgagtataag tccgttgttc ggcagctttt tcaactcccc
    21901 gatgaacgta acctaaacgg atagaagctc ctacgacaat ttccccgtct acggtgaatt
    21961 caaaatgtcc cggttccttc aatgccggat gttgcggtcc gataggaaca atatactttt
    22021 gctgaccatt gttattgaaa ttgtttactg tttcatcgct cattattctt ctccagattt
    22081 tgtttccggg ttatcctgta atccggaaaa agatttccga agcggaaaaa ctccatccgg
    22141 ccaattttcc ggtaatatga gtttggagcg atccggtgta tttcggatat caataccaaa
    22201 taattccata cattcccttt cataaagggt tgccgaagga ataatcgggc atattgattc
    22261 gaccgttaag tttgaatacg gcaaaaagaa acgtaatgtt ccgatcacat tttgttctcc
    22321 gaattggtaa accaatccga tttgcggggt accgttttcg tcagccgtat cataagccga
    22381 gatagtaatt aaatatcccc aatcatgttc gaccaatttt tgaaccgctt ttaataaatc
    22441 gttcgaatta atataaacat ccacccgatt cggttgtatc gccggaattt cagtttttat
    22501 tgccaaatcg gtaaatattt cctgaaactg attcagtaat tcctcttttt ccattattcg
    22561 gcttcacttt cttttttatc cgctgtctga gcagaatctt gttcgggtga ggggataatc
    22621 ataggctgac tgtaatccga tttctgtcgc ttccctctgg caatatcgag gaggataatc
    22681 aatccattaa tcattgcttc cggtctggga ggacagcccg ggacaaagac atctaccgga
    22741 ataacgttgc tcactccccc ttgaacaaca ctataacaat tttggaatac acctccgctg
    22801 atcgcgcagg aacccaatcc caaaacgaat ttcggttccg gcatttgatt atatattcga
    22861 atgaggcgat cttttgattg aagggtcacg gaaccggtga cgattaaaat atcggcatga
    22921 cgagggctcc cctgtaattt cactccgaat ctttcaacat cataacgggg ggtaatcaaa
    22981 gttaatagtt cgatatcgca accgttacaa gatccgctgt tataatgaat tgcccaaggg
    23041 ctgtttattc tcgcccaatt acgaattctg ttttcaattg ttttctcaga gccttttgca
    23101 ttcggatcga tatccgaaag atcgatggtc ttgttcattt tatgattctt ccgtttcaaa
    23161 atatatctct gtctgccaaa tcagcctaac atcagagcca ataaacctaa cattaataca
    23221 accgtataca ccagcgaagt cacactaaga ttgctgctgc ccataatcag cactcccaaa
    23281 tgaagtaatg caaaaaagag agaaatcagc aaaaaaggtt tataacccgg aacccctctg
    23341 gtttccggcg caacctcacc gcttccgtat aaagtccttt tttgatcgtc tttcgtatct
    23401 tcgaccggag cggtcttctt cccgtaaaaa accaataagg tgcctataat cgcatagatc
    23461 agaaaaccaa tcggaggact cagtaaaata tccataatat ttatttccta ataaaacata
    23521 gtatataaag tttcgcttgc gactttggtt attccggcgg taatacccgg gaagaaaccg
    23581 agaagaatga ctgctgcggt taagatgatg atcggaataa tcatcgcttg ttgaaacttt
    23641 ggtgaaatgg caacttcatt attgtttgat acatgccgat aaatacgatt aatcggaggt
    23701 gcgtaatgtc ctaaagataa aacactgtta ataccggcaa gtgctattaa aaaggcaacc
    23761 caagactttt gagcagctat tccgcttgcc agaatttgcc attttgaaat aaagcccgca
    23821 aataagggca atcctcccaa tgccagcatt gagattgtaa aagccagaga atataaagga
    23881 tatttcgcgg aaaccccgtt tagatcatcc aataataacg gttcgtggtt tcctttcgat
    23941 acttttatac aatataccag agcacctgcg gaaagaaagg ctaacccctt cattagagca
    24001 tggttgaaga tatgggtaaa aattccctct gcattttgag cgcttgcggg aaaagaaatc
    24061 gcaaagccga ttcccaaaag gatgtatccc atctggctga cagaagaaaa tgcaagcatc
    24121 cgcttgattt ccttctgtcg aagggcagaa aaagtaccga taaacatatt gatacatccg
    24181 atcaccaaga aaatagtccc gagggggaga tgagcatggc tgacggcact caaagcgcgt
    24241 aacaatgcga ttaaaccggt ttcgatcaca atgccggaaa gtaatgcact gattcccgcg
    24301 ggagcttgag aatgggcatc cggcagccaa aaatgcatag ggacgattgc acatttcact
    24361 ccgtagccga tcagtaaaca agctgttgct gcgattaccg ccggacttga ggttagaatg
    24421 ctttctctga ttgccgaaat tttgacttct cccgtcaatc cgaaaagaat cgaagttcct
    24481 aatattacta agaccgaacc agccgcactt tgaactaagt acttaatgcc ggattccaat
    24541 gattctgtac ggttccgata aaaactgacc aatgtgtaag tcgaaacagc cattaattcg
    24601 aaccaaacat acaagttgaa tatatctgtc gcggttgtca accccataat actggacatc
    24661 agaatcataa tttgaccgta gaatttctct tcatgatctt ctgccaccat gtattgagcg
    24721 gaaaataata caaccatagc cgacaacact aacaggaccg aagtcatgag aaggctgatt
    24781 ccgtcaaatg tgagggtaat cattcctgcg gttaaagaaa caggtccttc ttccgtaaat
    24841 ttttttgctg tcatcacaaa aggaacaacg gtcaaaagaa ttgaaaataa gcttaaccat
    24901 gctgccttgt tggggcttga ttcatcatcc ggcgggatgg ttcgtccgac aacataaaga
    24961 attaaagcgc aaattattgg agttatcaaa agccaaaaaa gcggattcat cggcatatta
    25021 cagccctccg atcatcagtg caataagaat acaaatcaat ccgatcagca tcccgaaaat
    25081 attccaattt aatgagccgg tctgaatatt tcggatcgct tcactggctg catttattcc
    25141 gtttacaaca ccgttattga ttgcttcaaa cccaaaactg ttcaacgaaa tcgactttat
    25201 cttccgagta atgctattat gtaacggtaa tttatttctc aaaaaccaaa tcagaattcc
    25261 gatcgccacc attcctaaag ctaaaaatgt atacggatcc ccaaagattt tactgaccaa
    25321 ttccgacaaa gtttctttcg gaataccgta gagaggtaaa ctgtttgaaa gggatcggct
    25381 gaatggttcg aaaacaagcc aagatgtaat cgttcccaat gacaaaatac cgagtggtaa
    25441 agccatgaga aaaggtgcgt catgtacatg caaatcggat ctcggttctc cgaagaacac
    25501 caaccataca caacggatcg tatataaagc ggtaagaatt acagagaaca acattaatat
    25561 ataaataata atcggtgtcc cggaatgaat ggattcgagg attaattcct ttgaccaaaa
    25621 tccgtttaag accggaattc cggcaagagc taatgatccg ataataaaag tgatgctgat
    25681 aaacggaatt ttcttaaaaa aaccgcccat tttatacata tctcgtgtcc cgcaggaatg
    25741 gataatagcg ccggcacaaa gaaaaagtaa tgccttgaaa attgcatgag aaagcagatg
    25801 aaattggcag gcaagaaccg atccggctcc gattgcggcg aacatataac ccagttggct
    25861 tacagtcgaa taagcaagaa cgcgctttaa atctgtcgca aagattgcca tcagagatga
    25921 aatcaagatc gtgatcaaac cgagaatcag gattgtcatt ttccaaccgg ggactgcttc
    25981 cagagcggga aagaaacggc aaaggagata tacgcctgcg ttgaccatgg ttgcggcatg
    26041 aatcaaagcg ctgacgggag tcggtgcttc catcgcatcc ggcagccagg tgaagaaagg
    26101 aaattgagcg cttttcgccg ttgcggcgat gatcagagtg aaaccgataa tccctaaaac
    26161 atttgccgga atttctccgc cttgcgctgc ctgaataaat cttccgattt caaatgagtg
    26221 tgtataaccg aaaatcaaca atccggcaac catcaatccg atccctccga attgcgtaat
    26281 gaagagtgct ttcaaaccgc cgcgtaccgc attggggtca tcattataaa aggagatcaa
    26341 ttgataggag cataatgctg ttatttccca gaagaagaaa atcataaaaa tattggcggt
    26401 cagtaccaac ccggacatag ccccgatgaa taacagtacc atggaataat atctcgcaag
    26461 acttttctca tgcttcatat attctgcaga aaaaatcact gtcaacgaac cgatacagtt
    26521 ggcgatacat gccaaaataa ttcctaaagc atctgttgaa aaggagagat taccgaaaag
    26581 gggaccgatt tttgcaaagg atatggttga gtctccgatc tgagagagca acagtaaact
    26641 cagaatagcc gaaaggatcg ctccggcact tgcaaattga tgcaatcctt cttcatgttt
    26701 atcaccgata aaccaaacca atatggcggt cagccaaggt gtgaaaataa gaaagggaat
    26761 aagtattccg gccattgttt atctcttcaa tttcgaaatg gaatgaatat cgcatgttcc
    26821 ggtcttttcg cgtagttgaa tggacattgc taatccgatt acagctacga ttgtgtccgc
    26881 aacaataatt gtgatcgcca tactttgagc caattccatc tgatcggtta attttccggc
    26941 caaaatcatc gctatcatag ctccttttac caagacttga agtgctacga ttatttttag
    27001 gatgtttctg gtaataataa ggcaataaaa accgattgcg atcaatgaca cgattgcaat
    27061 gattaccagg gtattcattt attcttcccc tccttaattc ttttggaaac cgcaggagca
    27121 atcagcaaca agattccgag tatacccgag atgaacaaag cgccctgcag ataaatatct
    27181 atttttctgc cttcccagaa aagatctgaa aaaatcggtg aggtcgtttc tgattggacc
    27241 gaaggcatat gctgagcaat caacaaataa gccagcatgc caaatgatac aaccgatatg
    27301 gatatcgcta accaatgagg aacaaaaaca tctgatgtaa ttttttcatc accgatgata
    27361 ctgatcgcaa agacaaataa gaccgttacc agccccgctc ccacactcaa ttcgattacg
    27421 gctaaaccgg gagatcctaa aatataaacc aatattgaag tcaggcagct tgtcccggct
    27481 aaccataatg cggcatttaa taatcctttc actctggtag ctttgtgagc tgagtagatt
    27541 agtgctgcga caaccagaaa atagaccaga ggcattagat tcctcccata taggataaaa
    27601 agggaaaatt aaaattatta acttctgtcc ataattatat atccattttt acgaaattcg
    27661 gaatatattt cgataaaatc cgttttttca tcgaaataag cgaataaaaa gatttaaatt
    27721 acctattaga gaaaaaaata acaaacaaag tttaatattt atcgtcatag taataataaa
    27781 aattcgtttt atcgtttatt tatgaaaata atcactaaag aaatcaggtc gatcatagcc
    27841 taaagcttat tttctttata taattggttt ttatggataa ggacgaggtt gaaataaatc
    27901 atatgaaata tttattaaaa ttggatgaaa aatattccga aaaatttcaa aaaaaatttt
    27961 caactccggt gcttttcagg attgcggcat ttttctcaca tacgggggat tcgtggcttt
    28021 ggtgcggcat attatttctc ttatggatat tttcgagcgg agaacaacaa aaagttttgg
    28081 ctttttgggg gctcagcatt gcttttacgg cgttttttat tttctttttg aaaaaagtaa
    28141 ttcatcgcag taggcccgaa ggcgagtggg gaatggttta tcgcaaaaga gatccgcatt
    28201 cttttccatc agggcatgct gttcgagccg gacttatttt gatgttggct tggaaaacct
    28261 tcggtatgcc atacttttta gtttttctga tctgggcggt tttgatgatt cattcgagag
    28321 ttattaccgg agtacattat ttactcgata tatatttggg tgttatattc gggttgctaa
    28381 tcggttgtat tcttatcggc tctcaagatt ggatttacca atcatttccg cttttatttg
    28441 accgatccgg atgggtttat attttttgaa taatatttca tagcctccaa ttgattataa
    28501 aagaggctat gaatttttat tggtatactc aagaaatcaa acggaaaaat aaatctattt
    28561 gtaaatggtt atcaaatctt ccaataaggc agcggcagtt tccatacgac ctgccccggg
    28621 tccgattaat gtgaccatac ctaacaaatc cgtctcatat tgaatcgcat tcatcgagcc
    28681 attaacattt gccaacggat tggataatgg aaccatttcc ggttttacag aagttattat
    28741 atggttctga actttcttaa ctgaaccgat caatttccat ctgcaattct ctttttttgc
    28801 catttggata tcttcgacgg ttattccggt aattccttca taacccattt cagccggtgt
    28861 ctgtttttcg tcaaatatca gttgtgaaag gatcgcaact tttccagctg tgtctaaccc
    28921 ttcgatatct ccgctcggat cggtttccgc gtaaccgaat tcttgtgcat gagcaagagc
    28981 ttcattaaaa cttaatcctt tttccatctc cgttaaaata aaattgcagg ttccgttaag
    29041 aatgcctcgt acggaagtga tgccggaagg aaaaagcaag tcttttgcga acctcagaga
    29101 aggagttccg ctcattactg ttccttcaat ccccagtttc acattatttt gattggctaa
    29161 actgttcagc attttataat gcaaagcaat cggccctttg ttgctggtaa ccacattctt
    29221 accggagaga atcgcttctc gaatatgcaa aaccgccggt tctccattga ttaaattcgt
    29281 cgaactgatt tccacaatta catcggtttc agcttgttga atcattgaaa ttgtgttcca
    29341 atccggtcgt tcaacatcac ttaattcggc gaatgaattt accgtaagca attcgtccaa
    29401 tgaaatgccg ttagggttat agacacaacc gttatttttc gtgacaattg ctgtaatgaa
    29461 aaaatttagt tgataaattt tacggtagaa atccgttttc tgatttaatg cctggacaaa
    29521 accttttccg acattgccat agccaacgac tccgattttg acatttttca tgagtgggct
    29581 cctttttagt tcattctgaa ttttattttt gccagtaatt ctccatttag aattgatccg
    29641 cctgccgctc ctctgattgt attatgagaa accgtaacca ttcgataatc caggatatta
    29701 tcttttcgaa ctcttccgac attggtagcc attccttttt ccagtgaacg atcccaacgc
    29761 ggttgaggac gattttcttc tttatgataa atgatacctt ttttaggaca ggatggaaga
    29821 cttcggcata tttccggaaa ttcaaaattt tccaaagttg atataatctg ctcttcggag
    29881 accttttccc gaaccgcgaa tgaaaggcaa atcgtatgtg catctgtaac cgctacccga
    29941 ttcgtatgag ccgaaattac aatcggatgt tgaacgattt tttcaccatc aaaatttccc
    30001 ataattttgg ccggttcata ctccaatttt tcttcttctc cgctgataaa agggatgacg
    30061 ttatccaaga tatccatacc gggtacgccg gggtagcctg ctccggaaag agcttgcatt
    30121 gaaaaaacaa ttgcttttgt aacgcaaaaa tgtttgtcga ttgcatttaa tgggacaaca
    30181 aagccgactg tcgtacagtt cggattcgtc ataatcgctc ccggccattg atatgttttc
    30241 tgttgaacat ctaatatttg tagatgatcg ggatttacct ccggaaccag aatcggtacc
    30301 agcggatctt tccgataggc agaagcattt gaactgacat atatgccttt tctggcaaaa
    30361 tccggttcga ctatcatggc aatttcggcc gggagtgccg agaaagctat ttgagatttc
    30421 ttgatcgcat ccgcatcggt aggcagcagc tccatttttt ttacgtattc aggcatcgga
    30481 tccgaaagaa gccagtgaca ggattcttca taagtttttc cgaccgatcg atccgaagct
    30541 gttatttcga ccacttcaaa ccacggatgt tgatccaata aactgataaa acgttgaccg
    30601 acactgccgg ttgcagctaa aactgagacg ggaattttat tactcataat cttatatcct
    30661 ttcatgtgat tagaatgaaa tcaattggtg aatcgccttg agagctgaaa tcgaatcgga
    30721 ggaactgaca ataatattca atgatacgtc tgaggctccg taagaaagag ctttgatttt
    30781 gtattgattc tcgattaatt tacttaatac ctgcgtgatt acttccggat gggatttaag
    30841 tccggaacaa atcacggtta agatatcaac cggttcgctg atttcaaatt catcaatatc
    30901 ttttcgcgaa atttctttct ccagaatggt tctgagtact ttttcaactt tattcacctt
    30961 ttttatatcg atcggaaaac acaatgattg ctctgaagaa ctttcaatca ccatcgggac
    31021 atttactccg gcttcgacaa atccattaaa tattttggct gatgcattca ttccggaatc
    31081 cattcctgca cccgaaacag ttatcaattt aaatcccgat gccgttgtaa ccgctttaat
    31141 cttaccgaca tccacggcgt cattattgtg aatcaagata gtcccttgac tctgtggtgc
    31201 aaacgtattg caaatccgca tctttatgcc attttccaca caaggtttga cgctcttggg
    31261 atggagtact ttcgctccgt aatatgccat ttccgaaact tcattatagg ttaatgcagg
    31321 aattgtcttt gcatcgggaa caattcgcgg atcggcactc atgacaccgg tcacgtccgt
    31381 ccaaatccaa acttcttccg cttccaatcc tactgcaata atagaagcgg aataatcgct
    31441 tcctcctctc cctaaagtgg taatggatcc ttctttcgtt gcgccgataa atccggtaac
    31501 aatcggggtt attccttcat tgagtatcgg atataggact tttttgcatc gaacggaggt
    31561 taattccata tccggagagg cgctttgata aacatcatcg gttatgatca attgagttga
    31621 ttcgaccggt tttgacttaa ttccggctga ctcgaccgct gccgataaaa tccgaaccga
    31681 caagcgttct cccaaacttg aaaccgcatc taaagcgcgc ggacttaatt ctccgagaat
    31741 cttgattgca ttgcataaat ttacaaattc ggatattaga cgatttacat ccatttttaa
    31801 ttgaagccag attttcggat cttcgaccaa tttgtcaatc aattcatgat gtctggcagt
    31861 aagctcatga gcggtttttt ctgcggtggt taaattccct gacgaagcat cggttgcgct
    31921 ttttatcaga gcatcggtgg ctcctgaaat ggcagaagta accacaacaa gattgggata
    31981 ctcttttttc gtttcacaga taattttgac tgccgatttc attgctacag gagaaccgac
    32041 ggaacttccg ccatatttca tgaccaatat atgatttggt atcgatgtgt tttccataaa
    32101 aaagacctca tttcgatata aaaaaagctg cccttttgag gcagctctta gttttatttg
    32161 aattcgtaaa gaatccgcct cttcaaggta gatagagcag agtagtcccc gtagtcgtta
    32221 cgatatattc aacaaaattt cggaaatctg tgtgtttcat atatgcatca actttaatcg
    32281 aaaacaattg ttttctgcaa atgaatttta aaaagatatg acaacatttc tgaaaaagtt
    32341 taatataatg agactgctta aatgatttaa agatcggaaa ggggaaaaat gatacctgtt
    32401 cttaatgcag caaaaataaa ttgtgattcg attcttccca gccggaagca ttccgatgat
    32461 gccggacttg atttatatgc ttccgagaca aaaataattg aaggaaacgg aattaatgtg
    32521 atcggtaccg gcatagccgt tgatattccg aaaggttatg tcggacttat ttttctcaaa
    32581 agcgggagca attttctgct gggaggaggg gtagttgatg ccggttatca gggagaaatc
    32641 aaaattaaga tatttaatat taaaaccgaa acaattcaaa ttgagaaagg acaggcaatc
    32701 gctcaattgt tgattgtccc gattgagact cccgaggttc gggaagttca aatttctgat
    32761 ttatttaaag aaaaatccga tcgtggagcg gaaggaggta tccatttacc gatctgatta
    32821 agaattattt ccgaaaaccc acggagagct gattttatcc caggtaacta tttcatccgg
    32881 ttcaaaccaa aggttaatct cacgctcagc cgtagtaaca gaatcggatg catggataag
    32941 attccggtcc gttttggcgc tgaaatcgtg gcgaattgtt ccgggagccg attcaatagg
    33001 ctttgtagct ccgacggtct ggcgaaccgc tttcaccgct tcttcacctt cccaaaccat
    33061 tgcaaaaacg ggaccggatg taatgtaatt gactaaaccg ttgtaaaaag gtttcccgat
    33121 atgttcggca taatgacgtt cggcgaattc tttcgaaact atcataaatt ttgcggcgca
    33181 aagaatgagc ccgcgatttt caaatcgttg aataatttca ccgattaatc cacgttgaac
    33241 accatcaggc ttgatacata cgaatgtttt ttccattttt atttccctct ttcatttatg
    33301 attttttgaa gttcttcatt ttccggaaaa agaattaaaa gtttctgcaa atcttcttga
    33361 accatttccg gttttatttg tttttcaatt aattgtttat aaagtcgaat accttctact
    33421 tgatttccgg tgctgaaaca tgtccatgca tttcgaacca tttctgtttc ttccttattt
    33481 tggagatcat tttcaaactc atctatatcc gctttacggt cagaatcaaa ttgtttttcg
    33541 gcagtacctt cctgttttat ttcaacccct tcggtgacca ctttttccag ccagtgaaaa
    33601 gcatcatcta aactttttgt cgagcgggat gattttccta tcttttgatc cttctcagtt
    33661 gactcacgct gcttttgaga attcttatct gattcgggaa tttgggattt aaaataatct
    33721 ctttggaaga aagaatcttc ctccaataaa attttctttc tttctgaaga ataaaataca
    33781 ttttcttctt cattccggtt attcgtctga taaagaaaat ccggacaaac cggcaataat
    33841 tttaaccaag aaggtaatgc ggaaagatca ttttcgataa tatgtgatcc gggctgttgc
    33901 caaaccgttc tcaatttcga gttttgaaaa cctgtccatt cctgatagat cacttggatc
    33961 gaattattcc cgcaggttaa ttgattatct tgtatggtat attcggaata atcgggattt
    34021 aattcaatta atctattgag gtatttcata aaaagctgag ggttcttttt ttgccaatag
    34081 atggcggttt tctgtaatgc tgattgcaga taaggtgatt tttccaaaat gatttggagc
    34141 aattttattc cctcatcctc ttgatgattt tcaaacaaac ttagtccgat aaataaagat
    34201 ccgaaggtct gataactttc aagtgaatta atttgcagct cattgattgc tttttcatag
    34261 tttctttcgt tataaaagtg aattcccgct aaaagatgag aaaaactttc cggattggga
    34321 taatttttca tccggctgag caatcgctga tataaactga caacctcttt attttccggt
    34381 tgcaaaaata aagcggcttg cgaaaaaaat atggcttgat ccggcagttt taattcttcg
    34441 aaaataaaac cgagtgctaa atggtttaaa aaattatccg gctctgcaga aacgatccga
    34501 gaataagtat ccagagcagg tctgagttca ttcatttcca ggaatattct tcccaataat
    34561 ttataaaggg ataataattt gggaaattgt ctcagaagat aaaaacaatg atatgcagcc
    34621 tgttcatatt tgctttcaga cacaagcgta tcaattctcc cgagataatc attcaattct
    34681 atcttagcca attaaattcc tttcgttctt attttaaacg aaatatccaa acaaaaaagc
    34741 cattcagcaa gatcaatgct gaatggcttt ttgtttggat taattaatca cctaaatagt
    34801 ctcttaagat ctttcgaacg gttggatgtc gcagacgact caatgcttga gcttcaattt
    34861 ggcggacacg ttctcgcgtt acgcccatct ttcgtccgac ttcttccaaa gtgtacgctt
    34921 gcccatctaa caaaccatac cgaagttgca gaattcgaac ttctctcggt ggcaaaccat
    34981 tcagcacttc atctaaatgc tctttaagta aattatatgt agcggtatcg tcaggcgccg
    35041 gagcatcttc atcttcgatg aaatcgccta agacggaatc ttcctcatca tcggtaggtg
    35101 tctccaaaga gagcggtctt ctcgctactt gaatcatgtt ttcgactttc ttcgggggaa
    35161 catccaaggc atccgccagt tcttccacag aaggatcgcg acccaatttt tgcgtcagtt
    35221 ggtgttgtac acgcagtaat ttgttaattt ggtcgcccat atgaacgggg actcgaattg
    35281 ttcttccttg atctgcaata gcacgtgtta ctgcctgacg gatccaccat gttgcgtaag
    35341 tgctgaattt atgccctcgt ctataatcga atttctttgt agctctgatt agaccgatat
    35401 tcccttcttg tatcagatcg agaaaaggaa cacctcttcc catatatttt ttggccacgc
    35461 tgataaccaa gcgggaattt gcggtaatta aatgctctct ggcagcccaa ccgtcttcaa
    35521 tactcttacg aagctccaag cgacgttccg gagtgacatt tactcgggca agttcttctc
    35581 ttgccattct accgcgctca atacgttggg caagttcaac ttcctcatcc gcagtcaaca
    35641 atgggactcg gctgacttct tttaagtaaa gaccgatcgt atcatcggta tctatattgg
    35701 cgagatcatc cattgaaata ttcggaatga tctcattttc ttcttcggcg aggtcaattt
    35761 cctcttcggg aggttcttcc agattggact cttccgagta cggaattcct gcgctaagca
    35821 atgcggaaaa agcctcttca agttgttcga catcttgttc ggcatccggg aataatgcga
    35881 gtatatcgtc tatagtgacg tatgatttct gccggcccag ctcaattaat ctggcgatag
    35941 ccggataatt ctcatcatca tcttcttcat caacatcgat attgagtaag tcttctttca
    36001 ttttgttttg atatcctcta tcagtaatgt actctacaca gaatacactt atttttaata
    36061 aaattcaatc cctaaaattt gattttttca cggtttccct taaaataatc taaaaatgcg
    36121 catagccact tatttatcat accacaatat atcaaattta gaatatatat tctattatac
    36181 tttcattaaa aagctgccgt tctatgacgg cagcttgaaa atcactaaga aatattaaat
    36241 ggtaaaaaat gaaaccaaaa cgaccctggg acccgcatga accataaaag caggaggagc
    36301 attaaaaacg ggaacattct caaatccaaa acgttcctta aattcttttc tgaagaaaga
    36361 caattcctct tgacataaat tcacatttaa agaaagtgaa attaaaccat gattcccacc
    36421 cttcgggcat tcggcttgaa ccaaatcaac aatacgtaaa agggcttttt tctgagtcct
    36481 ttgtttttca aaagcttcaa cttgtccgtc tttcaaggtg aggatcggtt taatttgtaa
    36541 agcgtcgcca aaaagtttag atgctccgcc gattctgcct cctttataaa ggtattcaag
    36601 cgtatcgaca accgcataaa aacgaacatt cttatttaat aaattgatat agttttcgat
    36661 ttcatccagc gaacgatgct gatccgccat ttttttcgct tcgattaatt ccatcgccac
    36721 aggaccgccg acaacacctg tatcgataac acggatatcg acatcaggga aatcattctt
    36781 ggctgtcatc gcacttcgat aagtaccact cactttaact gatggagcca ataccaaagc
    36841 atcttcttta ttttctcgga gtttttcaaa aataggggta taaagcgaag gaggtggtgc
    36901 tgctgtcttt ggcagttcct tcgatgcttc caacattttt acaaatgtga ctggatcgat
    36961 ttcataatca tcacggtaag atttatcacc gaagataaca atttgaggaa tataatcgat
    37021 gcctatctct ttcgcttgtt ctggagttaa tcctgatgtc gtatcaccta taatgcgtgt
    37081 cattttattc cctttcagta gataaaaaat ctctggcagc cggtattcca ccaactgagt
    37141 cagcaaatcg aatcgcattt actatcgctt gcgcaacaac gatcggcgca aatgccgtaa
    37201 cagcatgaat gtttccattc tttctctgtg atgagacagc aaacattgta tccccgtcaa
    37261 acatagtatg agcgggttga atactgagaa ccaatccatt ctgtccgcat tgagccaatt
    37321 tgtttgcctc gtcttttgaa agctttacgt ttgtcaaaac acaaccgata acggtattat
    37381 ttaccggaac agtttctaaa gatttttgat ttgtttttcc ggacaagatt gacagcgtat
    37441 ccgcaaataa tattttgtta tttttgtctg ctgcggattc gtcgaccagt tgggcaccgg
    37501 cgagaatgtt acgattattt ggatccacta catcacccaa tgaattaacc gcgacaatcg
    37561 cccctaccca aatccccggt tcgagttcgc atgcggcgtg gccgattcct cccttcatac
    37621 aatgatccat tcccaatatt tttccaaccg ttccgccggt tccggcacca taattccctt
    37681 gtttgggact atctgccgaa gcattcaaac aagcctgata tcccatcgct tgatcgggtc
    37741 ggatcgaata actcccgatc gtaagatcca tcagaaccgc agtcggaacg atcggaacga
    37801 cagtcggtcc gacatttaca ccttgatgat gctcttcaag gtatttcatg acgcctccgg
    37861 ctgcatctaa cccaaaagca gagcctcccg taagtaaaat cgcattaaca gtttcaacca
    37921 tatggagggg gcgtagtaaa tcggtctctc ttgtgcccgg tgatccgcct cgttgatcaa
    37981 cgccgccgac acaaccttca tcgcaaagaa taaccgtgca tcccgtaagt gcttccgaat
    38041 cttgtgcgtg gccgatcttg aatccaggaa ttttgttaat cggttcaaac ttcattgaaa
    38101 ccttttcgat ggaattaaaa tttataaaac attccctaga ttataacaag atttctaaaa
    38161 aataagagaa acacctgaat atgttccgaa gagctgttca aatgaaatat agtaaaatta
    38221 acttcgaaaa aaataatatg ggattttatt ttgtgaaaaa tgttcaaaaa tctttagaaa
    38281 aaaatgtatc taaacaattt atcctattaa taataacggc aatatatata ttaataaata
    38341 ataactctgt tcaagcccaa attcctacgc tgagcattcc tacggtaacc ggtaccgatc
    38401 aaggtgtagt cgttacggtt aagaatgatc cggcgaatcc tcaaattaat ttaagggctg
    38461 gtcccggtac agaatatgat aaagtcggga cgatggtgat cggccaaaaa gccgtcgcaa
    38521 aagggcgtac cgaaggggga acttggctgc tgatagaata tccgggagct ccggggggat
    38581 acgcttgggt ttattcgact tatgttgatt atttgggtga tttgccgatt gtgaccatac
    38641 ccaatacgcc tactcctcgt gttacaaata ccattgatcc gactttagcg gcacaattta
    38701 tcataacagt ggagccgacc cgtcttccga cttttacgca acctgctcct ttagcaattc
    38761 ctacttttcc gcaaagcagc agtacctatg aaagctccgt cccgatggga ttgattattg
    38821 tatcctgcgc gattttagga attatcattg gtttattcac tttggcgcaa aaacgctgat
    38881 aataaaataa gttcttactc gaggtgatat ggcaggaaat agcaatccgg tagtagtcgg
    38941 cattcgattt ggaaaactat caaaaattta caattttgat gcgaccggtt tggatgaaat
    39001 ccatctgaaa gatctggttg tagttgaaac gtgtaaggga aaacaaatcg gtgaggtcgt
    39061 taaaatttat caagattatg aaatgaaaga tgaaccctta aaaaaagtgg aaagaattgc
    39121 gaccgcagga gaattagtat cgaatgaatt ttggaattta aggaaaaaag aagtattgga
    39181 atattgcgaa aaaagggcgg cggaattaaa tttaaaagat attcgaatac tgaatgctga
    39241 atttaattat gacggatctt atatgatctt aacttattca agtccgacgg aagaaaaagt
    39301 cgatttgaaa tcattgaaat atgatatcca aaaaagtttc tcaatcaata atatagaaat
    39361 caaacaaatt ggtcctcgag atgtcgcaaa agttatcgga ggcatgggag catgtggaat
    39421 tccatgtcgt tgttgctctt cttttctgac tgatttcagc agtatttcga ttaagatggc
    39481 caaagagcaa ggaatatcgc ttaccccttc cgaaattacc ggaatgtgcg gacgattgcg
    39541 atgttgttta gaatatgaaa atgaatatta tgctgaatgt cggaaaaacc tccccaagaa
    39601 gaagaaaaaa gtcatcactc ctcagggtga agggaaggtt gtcgaaatct ttccgttaag
    39661 acattccgta cttgttgaaa tcccatgtgt cgggaggagg gagttcaccg cagaccaaat
    39721 taaatatgac gaaattcccg ctaaacaaga cgatagttca aaagaggaag aagaggcaaa
    39781 atgaattcaa tgaaaatcgg ttgtcatctg tcggttgcgc aaggttatct tcatatgggt
    39841 aaagaagcta tcagtatcga tgcgaatact tttcaattct ttacgcggaa cccgcgaggc
    39901 tggaagacca aagaattgaa tattcaagat atggaagatt tgacaagtct gatgaaatca
    39961 aacgattttt ctccgatttt agcccatgcc ccttataccc taaacccctg cgcagcggat
    40021 gaacgtgtac atgaattcgc tttgatggtg atgaaggaag atctggctat tatggaacat
    40081 atgccggaga attattataa ttttcatccc gggagccatg tcggacaagg aatcgaagcc
    40141 ggtatccagt tgattattga agcgctcgat catatgttat atatggagca aaaaacaact
    40201 gtcttattgg aaaccatgtc cggaaaaggc agtgaagtag gcagtacttt tgaagaactt
    40261 caagagatta ttaaaagagt aaaactgaat gacaaacttg gagtatgttt ggacagctgt
    40321 catatttatt cggcgggata caatattgtg gaagatttag acggtgtgtt ggagaaattt
    40381 gataaggtga tcggactaaa ccggttgaag gcgatccatt tgaatgacag tatgacaccg
    40441 ttcgggagta aaaaagatcg tcatgcaaaa attggtcagg ggacgatcgg gttggatgct
    40501 attattcgtt ttatcaatca tccgctatta aaaaatttac cgttttactt agagacccct
    40561 aatgatgttc caggatatgc cgaagaaata aagatattgc ggcaagctta taatggataa
    40621 ataaattaat aaaaaatgcc ggatcttttt tttcgtccgg catttttctt tttaacagtt
    40681 gagaagatta tttatcttgt agtgccgcga caccgggaag aacaataccc tcgagcattt
    40741 caagagaagc tccaccgccc gttgaaatat gagtgatttt atccgcgaga ccggattcct
    40801 gaatcgcagc cacagaatct cctccgccga taatggaaat agaattactt tccgcaactg
    40861 cttttgcgat ttcaaatgtc cctttcgcaa aattaggcat ttcgaagaca cccatcggac
    40921 cgttccatac cactgtggcg gcatttttaa tcaggttgct gaaaaccttt atggtttcag
    40981 gaccgatatc taacacacgc cagccggcag gaacattccc aacaggaata gtttttcttt
    41041 cagcgttgtt atcaaaagca ttggctataa ccatatcaac cggcaacact aatttgttac
    41101 tgcccatgtt taaaatctct ttagcggttt ccactgcctc atcctcaacc agagaatcag
    41161 ccatatcaaa tccttgtgct ttaaaaaagg tatttgccat tccgccgccg atgagaactt
    41221 tatccgcttt gttcagcaga tttttaatca cgccgatttt atccgagatt ttagcgccgc
    41281 ctaaaattgc gacaaacggg cgtttcggat tttccacggc tccagaaagg tattgtattt
    41341 ccttttccaa aagaaagccg gctactccgg gcttgaaatg agtaactcct tcggttgagg
    41401 catgggctcg atgtgcggtt ccgaaagcgt cattaacata gatatcagct aaatctgcca
    41461 attgctttgc catttccaag tcattttttt cctcttcggg atgaaaacgg gtattttcga
    41521 gaaccagtac ttcacccggc tttaagtttt cggccgcttt ctttgccggt tcaccgatgc
    41581 aatccgaagc aaaatagacc ttattgggaa ttaatgttcc gagatattcg gcaacaggtt
    41641 tcaaagaata tttttcttcc ggaccgtttt taggacgtcc gagatgtgag caaaggatga
    41701 cagcggcacc ctgatctaaa agatactgaa ttgttggtaa agctccgcga attcgagtat
    41761 catcaccgac ttgtccatct ttgatgggaa cattgaagtc aactctgaca agaacttttt
    41821 tcccttttac atcaatatcg cgtatggtct tcttattaaa catgttcgtt tccctttctt
    41881 ctgaataacg ataagttttt atcaagcaaa tcagcgactt ataagaaaaa atgatcggct
    41941 gaaagttttt gcagttccga tcattttttc tttcaagttc ccgcactttt tgatgcggat
    42001 aacattttta ttccttcaaa acattcagat gaatcagaga ctcttcgaca tcatcaaagc
    42061 cagatcacaa gctcggttcg aatagcccca ttcattgtca taccaagata caatcttgag
    42121 catctttccg cccattacgg atgtctgcat caaatctaca attgaagatc gagaatcacc
    42181 gcggaaatca gtcgaaacca acggatcggc atatgtccct tcggtaactc ctaaaatgcc
    42241 ttttaatctt cctttgccag ccatgctgcg gaataaatca tttacttctt caaccgttgt
    42301 tgatttagag atattgcaaa cgaaatcgac gattgataca gtcggagtcg gaactcgtaa
    42361 cgaataaccg tcaaaccgtc cttttagctc gggaatgacc aatgccaccg ctttcgcagc
    42421 tccggttgta gtcggaataa tattcaaacc ggcagcgcgt gaacgacgca tttccttctt
    42481 atgaccttga tccaagataa cctgatcatt cgtatagctg tgaatcgtgg tcatcaaacc
    42541 atattcgata ccaaacgcat cattgataat ttttgcaacc ggtgctaaac aatttgttgt
    42601 acaggaagca ttagaaacca catgatgttt cgccggatca tatttatcat cattaactcc
    42661 catgacaatg gtgatatctt cattttttgc cggggctgaa atgataactt tctttgctcc
    42721 gccttttgtt atatgaacat tggcaccggg tttccctttt tcgggatcac cgattgcatc
    42781 tgtgaatata ccggtagatt cgacgacgat atcgacaccc aagtctttcc aaggaagagc
    42841 tccgggatct ttttcggcaa aaactttaat cgtcttgcca ttaatgacca gatcgttgcc
    42901 tacaacatca acggatccgt caaattttcc ataggttgaa tcatatttga aaagatgtgc
    42961 attggttttt gtatcaaaaa gatcattaat agctactact tccaattcat ccgaataatt
    43021 atcgatgaat gatttgagaa cctgtcggcc gattcgtcca aaaccattaa tacctacctg
    43081 aactttcatt gattcctcca aaattgcata aaagattaaa atttatttaa tgagccacta
    43141 attttccgtg gaacctaatt ctttataagc ttttaaaaga gcttccgcaa ctttatttga
    43201 gtcgtgcctc caaggatatg ctttatcgat aagatcatct tgaaaaacac gatattctct
    43261 taaaattgac tcctcaattt taacccagga aatatttttg gattctgctg cctcgtgata
    43321 attattacaa actacaccgt caatcgagac atctggaatt gcattatgga tgactttaac
    43381 atgatcataa caggtgaaat tagtcgtttc gccgggttga gtggaaacat tgcatacata
    43441 aataatcttt ccgtggcttg cttttaatgc ctgagcaata ccgttaactc ttaaattcgg
    43501 aagaacactt gtataaagac tgccgggacc caatgcgatt agatccgcag ctaatattgc
    43561 tttaatggct tccggattgg cgattacatt tttcggttca atttcaaggt tgcgaatcgt
    43621 gccgtttttt tctgtgatat tggattctcc gaaaactcga atgcactctt ttttcccgtt
    43681 gttttcaatt tcaacatccg caacgaggtt gacatcttcc aatgtagagg gagtaacctt
    43741 cccgtgaatc gcaagaatcc tggttaattc ttggattccc agttcaaaac ttcctgtaat
    43801 gtttgccaaa gcggtcaaaa gtaaatttcc taaattatga cccccaacac ttgtttccga
    43861 agaaaagcga tattgaagaa cttgggataa aaactcttca tcatccgata gagcggcgag
    43921 gcagtttcga atatcaccgg gaggaagaac acccagatct cgcctgagag cgccggaaga
    43981 acctccgtca tccgagacgg taacaaccgc ggtaatattt tctgaaatcc tttttaaccc
    44041 acggagaatg gttgacaaac catgtccacc tccgattaca actatcttcg tgtcatggtc
    44101 catttttgga tatttatcga actgattatg atcttccatt ggtccaaata atagcattca
    44161 agagatcgaa tgtaaagacc tgacaaacac atcctcattt atttataaat atattcctga
    44221 actcgggaaa gatcattgaa aagaaaataa agatttgatg agaagaatat tttagaatcc
    44281 ggtttcgcga ttattcccat ccggaacgca aattgccgga actgacctga tataataaca
    44341 aaaaaagtcg aggtttctat gaacgtgaat agttataaat atgatgatga atatgatcaa
    44401 tatacagatc catataattg tgatttggaa atgataatcg aagaggctga aggatatcaa
    44461 gttataacaa cttataatgc agagggtgtg attgtagatg ttcaagctca ttccgcagaa
    44521 gaaagtgcgg aaaaatcatc ggttgaaaaa ctggcggaaa tgattgatca tacgttgtta
    44581 aaagcggatg cgacaccgga tcaaattatg actttgtgca aagaggcacg tgaaaatcat
    44641 tttgcctccg tttgtgtcaa ttcttgttgg acgagattgt gcgctgaaat tcttcgaggc
    44701 agcggtgtga aagtttgttc tgttgtcgga tttcctctcg gcgctatgat tccggaagca
    44761 aaagcattcg aagcggaatg cgctttagaa aacggcgcat cggaaatcga catggtgata
    44821 aatatcggtg ctttgaaagg acgggatttg cgtttggttt caaaggatat tcaaggtgtc
    44881 gttgaaatcg ctcatcaatc cggagccatt gtaaaagtga ttatcgaaac ttgtctttta
    44941 accgaagaag aaaaaatcat tgcttgcttg atatgtaaaa ccgcgaacgc tgattttgta
    45001 aaaacttcaa ccggtttttc gacgggaggc gcaaaagttg aagatataga attgatgcgc
    45061 cgtgtagtcg gtgatgaaat gggtgtaaaa gctgccggcg gaattcatac cagagaagaa
    45121 gcggaaaaaa tgattgcagc cggtgcgacg cgtattggag caagtgcggg agtaaaaatt
    45181 atccatgaat gaatcgtccg aaaaacttta ttatggaaat atcgaatctg acggcattaa
    45241 attgcattat gccaggacaa gtttagggct tcggccggtt gtatttttac acgggatgac
    45301 ggataacggc tctatttggg gacagttagc tgccgcctta tatcagaaat atgatatctt
    45361 tcttccagat gctcgcgggc atggtctttc gcaaggagga gatattgaat attcgctaac
    45421 cgcaatgacc gatgatgcgg cgaattttat tcgattgctg gaattagatc ggccggttgt
    45481 gatcggtcac tcgatgggtg catctgttgc cgggaatctt gcggcggaat atccgaattt
    45541 ggtgagagcg attatattaa tcgatccgac atggtatgaa gcagaatatt taactgccga
    45601 atttgcgaga caagttattt ccgaatttga aaaagcatat gaagaatatc atcaattatc
    45661 gggaattgag atgattgaat atgcaaaacg gcatcatccg aattggtcgg aatctgatat
    45721 tttgccttgg gcaaaatcaa agcagcaata tgatcgcaga ataatcggga tgatcccgac
    45781 attggcagaa aattggcctc agattacctc aagaataaat attcccgctt taataatgac
    45841 cggtgatccg caaaaaggtt ctattgtttc acaaaaagta tcagaagaac ttttaatgaa
    45901 acattccaac tgggaatcga tttattttga agatgccggt cataatatac aacgcgatca
    45961 atttgaaaaa tccagagatg cgatcgttaa gtatttagct gaaatatact cataaggtaa
    46021 atataagcag atgtgcggtc ggttttcact tatggttcag ttgagcgatt tacagctcga
    46081 gttcggactc gaaagaatgc cgtcgtcttt tacgccttca gataatatct cacccggaag
    46141 taatataccg gttataacga atcaaaatcc cgataccttg gattattttt attggggatt
    46201 gattccggct tgggcaaaag atatttctat cgccagaaat acgtttaatg cgcgagcgga
    46261 aacgctgtcg gaaaaaccgt cttttaaaaa tgcgttcaag agaaggcgtt gcataatccc
    46321 tgcgaccggc tattatgaat ggaaaaaaac cgagacggga aagattccgt atttatttac
    46381 cgtaaaggag ttatcgatta tttcattcgc ggggttgtgg gaatattgga tggacgcaaa
    46441 cggaaatgaa atatattcga caacgattat cacttgtccc gcaaatgaat atgccgccgc
    46501 ttttcatgat cgaatgccgg tcattctgaa acgggatcaa agaaaaatat ggctcggcga
    46561 aagtcctgaa agcgaattgc gattattgct tgagccgatt cattccgatg aattgaaagc
    46621 gaccccggcg gatttataac agaaagctct attcggatcc gaacagagct ttctgttgag
    46681 tttcgattaa aaacatcaga caacgatatt gattaatttt tttggaacca tgatgatttt
    46741 ttgt
//
LOCUS       DAOSMM010000011        43809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_913_length_43809_cov_13.653655, whole genome shotgun sequence.
ACCESSION   DAOSMM010000011 DAOSMM010000000
VERSION     DAOSMM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 43809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43809
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_913_length_43809_cov_13.653655"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..477
                     /locus_tag="PK898_03120"
     CDS             55..477
                     /locus_tag="PK898_03120"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOU19057.1"
                     /translation="MLNNESLTELSPSQKIDAQINELGEDWRGQKLKEIRQLIKQTDP
                     EIVEEWKWRGTAVWSHNGIICTGEVYKNVVKMTFAKGALLPDPDHLFNSGLEGNIRRA
                     IDFHQDDEINKIALSQLIRDAITVNDSSRKSVHKSESI"
     gene            complement(618..1535)
                     /locus_tag="PK898_03125"
     CDS             complement(618..1535)
                     /locus_tag="PK898_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOU19058.1"
                     /translation="MPFLIIQQDITKIKTDAIVNAANTALRMGGGVCGAIFRAAGAAE
                     LQAACDKLAPIQTGDAVITPGFRLPAKYIIHTAGPIYRDGKHGEAMQLRSCYSNSLKL
                     ALKNGCKSIAFPLISAGIYGYPKKEALSVATSAIRDFLAENEMEIYLAVLDKSSIVVD
                     ENLLKNLSEYIDRVYEKPSGISNEGEGDSVKRNVEPFLEILTQIVKKSGLAEIEVCTK
                     ANLSRDWFSKVGSKGGSFVTKQTIIALSVALKIPFEETVELLAQTGFNLSRDQLFDVI
                     VSYFMRNQLYDIDLINEVLFLYDQQLLGS"
     gene            2132..2686
                     /locus_tag="PK898_03130"
     CDS             2132..2686
                     /locus_tag="PK898_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOU19059.1"
                     /translation="MQDRSRKIAVTGVLGAVTILLGLTHWGFIPWFGGVSLTIMHIPV
                     IIGAILEGPAVGAGIGLIFGLFSMLQAAIAPSGPTDVWFTNPLLSILPRLMIGPITWL
                     VYHSLKHWQAGAIIISAIAGSATNTVLVLGMMGLLGLLPWQVLGGIALINGLPEIGAS
                     ALISFLVIVAVRQIKVGKRQGSEV"
     gene            complement(2699..3517)
                     /locus_tag="PK898_03135"
     CDS             complement(2699..3517)
                     /locus_tag="PK898_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOU19060.1"
                     /translation="MNDFDFLSKTTIGQYIPTGSILHHLEPGSKLIGFGILILAMTFS
                     PSIIGVGIGLIASLIGLIIAKIPLRFALKGILIPLPFLIVIALIQIAFFPIRGQSVLF
                     RVGPIQVSILSLMSGLLLIFRFSALILAISLMTFCVSASEMIHGLQHILHPLNALGIH
                     TMDFVMMIQIALHFLPLLAQSAERIAKAQASRGAEWGIKSKGLLSRVKQIFPLLIPLF
                     TISLTRAETMALAMDARAYGFKANRTSMVEYSFGWREAVFLLIVLWIVVSIIIF"
     gene            complement(3510..5255)
                     /locus_tag="PK898_03140"
     CDS             complement(3510..5255)
                     /locus_tag="PK898_03140"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOU19061.1"
                     /translation="MKPLIQTDHLTYRQPGIEEEFPPILKDINFTIYPGELIALVGAN
                     GSGKTTLARQLNALLLPTEGNVYINGENTRNRQAVPKIRSEIGMVFQHPEDQIVATIV
                     EEDVAFGPENLCRLPKTIREQVNRSLAVVNAESYRYRQPHMLSAGQMQRVALAGVLAM
                     QPNAIIFDETTAMLDPIGRRDVLQCMGDLNRMGITIIFITHIMEEVNLANRVLLLNEG
                     SLIFDGAPEVLFSDPNLLDTGGLEQPTEYRIYEAFPDWFTGFKGKKIDLKDLFGAIPD
                     FTGPFRQRDKFTPRFSQPAIDEIEIQNLSYTYLAGNILQQQALYDVSMTVPKGFPHGI
                     IGATGSGKSSLLQHLNGLYLPQSGRVKVGPFDLNSMNFDLRALRHYAGLVFQNPESYF
                     FKQYVGDEIAYGPKLFYGTENLRERVKWAMNFVGLDFETFKDRITATLSGGEQRKVAL
                     AATLASHPSLLIMDEPTCGLDPRSRRNLIENLRSLQKNEMQLIISTHNMDDIVELAEN
                     VTVLSEGRSISNLPVSQVFSDREKLESVGLDQPISVRFAQCLRDKGWPISSDAVTLGE
                     IFTEVNDLLSFNKNE"
     gene            complement(5525..6259)
                     /locus_tag="PK898_03145"
     CDS             complement(5525..6259)
                     /locus_tag="PK898_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU19062.1"
                     /translation="MNKFSTQVNTQTVQRLLIEINEGEYRMLDRLPPEIRLAEHFGVS
                     RSTMRDALATLEREGFINRIHGIGTLINRQVLEVQTRIDLEEEFLDIIRNAGYRPGTV
                     YSKVEYSTANSMEIKKLCLEKGSQMIRVTRLISADEVPTIFCIDSFSSSLIRNMNYEE
                     SELHKPIFDFLKTYCEEDVFFDLTEIHAVNADKLLAAELQVSKGTALLHLDEIGHNVL
                     GKPILYSSEYYREGMITHTILRKKID"
     gene            6511..7512
                     /locus_tag="PK898_03150"
     CDS             6511..7512
                     /locus_tag="PK898_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOU19063.1"
                     /translation="MKKSNMYLIVIMVLIAIVTCLTGWQKADAADKLKIALVLPGKKD
                     DVSFNQAMYRGMMEYAEKHADEVDVKVVENIYDVADIEPTLIEYANEGFDVVIGHGYQ
                     FSEPVITVAKMYPEVAFLLGCGVGDAPNVIRYDVQLEAGGYLMGVVAALATQSNKIGV
                     IGGGEGSEITRGHEGYKAGARSINPDIEIQEVYTGDWNDTTGAYEAAIGMYDSGVDVI
                     WHSGDGIGLGVVQAAEEKDKYVLGNVEDQFSLAEKNVLSGLQYEWGNALENIFAMIKD
                     KSFFVEGKDTVMINVANGGLSMTELNDVKGWLTDEDKAIIADVYEKLGQGAIELPEV"
     gene            7589..9115
                     /locus_tag="PK898_03155"
     CDS             7589..9115
                     /locus_tag="PK898_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19064.1"
                     /translation="MDDQCVVEMRGITKIFNGVKANDNVDFFLKKRSIHGLLGENGAG
                     KTTLMNILYGLYQQEEGEIFINGKKEDISSPLKAISIGIGMVHQHFMLARPLTVTENV
                     MLGKKSRRGILLDTNQTSNELKALSDKYQMDIDPNSKIWQLSVGEQQRVEILTAIYMG
                     AEILILDEPTAVLTPQETESFFETLRQMRQDGKSIILITHKLEEIISIVDEVTVLRDG
                     KMVGRRTMDASVTKDDLCRMMVGRNVLFDFPPAETEPGEIKFSIRNISVKNDKGVQAL
                     QNFSLDIRRGEIVGLAGVDGNGQKELCEILTGLRPAESGTLQLDGKDVTNRTPNYYIK
                     QHISHIPEDRQTIGLALNWNLKNNLIIKQLGKGPFTRKGFIEEKLVDQNWEKARDAYQ
                     IKAIDGNELARSLSGGNQQKLILARELSLDPHVLVANQPTRGLDVRASEYVRGQILKA
                     RNSGTAVLLVSADLEEILQLSDRIAVIYDGQLMGILPRGADPMDIGALMMGKKQEVHD
                     "
     gene            9115..10164
                     /locus_tag="PK898_03160"
     CDS             9115..10164
                     /locus_tag="PK898_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19065.1"
                     /translation="MNEKTRKTAINAFGTICMILIALILGGILVYLTGNSPFEAYGAM
                     LRGAFSSPQRISELFVKLIPILLMAFGVSIAYKAQLWNIGAEGQFIMSSIAATAVALY
                     INIPIPVRFILSFIASLAAGALYAGLAGYLRNRFGANEVITTMMLNSIASYFLMYLVN
                     GPMQDPTTDLSQSPLVPESMFFARLIGNYRLHIGIFILLAVTIMMCFFWKSSVGYRIN
                     LIGQGEMVATYAGINVKQTVMTTMMISGAFAGLAGWIETFGIQHRILDGIAADYGNIS
                     TIIALLGSLNVYGIIASACFFSVLLCGGASMQRMTEVPYSVVNVIQGLIIILVIARNM
                     FADRLPQVSHKEKKG"
     gene            10169..11110
                     /locus_tag="PK898_03165"
     CDS             10169..11110
                     /locus_tag="PK898_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19066.1"
                     /translation="MGSILNITFFVSLFAGMIRLATPILLPALGQIYTQRAGILNLGV
                     EGTMLMGAICGFIGACVTQNLWFGFLCGILAGMIYSLIMAWLSITMRANQVIAGIGMN
                     ILASGLASFIYRLVFGIRALPAKITSFQSLKIPVLGDIPYIGTIFFQHNILVYLTFIL
                     VPITWFILERTTYGLKIKAVGENPRAADSKGIDVARIRYSAVLVGGAYAGAGGAFMTI
                     AYLNTFTEKVIGGFGYIAVSVVIFASFQPVRAMFGAMLFGLASALQLRLQAHGVGIPS
                     QLLLMLPYLMTIIALVIASGKAVFPSAYTIPYSRMER"
     gene            11107..11592
                     /locus_tag="PK898_03170"
     CDS             11107..11592
                     /locus_tag="PK898_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19067.1"
                     /translation="MNHRITLLFITIMLMTQSAWCYVAPTEERAMERAQAYAEVIDHS
                     YKHPEKIYAMLEKSFHDQMDEKSFCNAFAKERSYPYITPLYISKPHLTFDGNIAHVVY
                     DQAARLSGMTYKIDLIFENGDWFVRDWEEFLDGSYLEKFENIPYSLDWYYDTQKIEKG
                     N"
     gene            11596..13932
                     /gene="xdhA"
                     /locus_tag="PK898_03175"
     CDS             11596..13932
                     /gene="xdhA"
                     /locus_tag="PK898_03175"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HOU19068.1"
                     /translation="MGIKKSVTRIDALMKVTGQAKYIEDLVPRDALHVKIVHSTIANG
                     VVKKIDSEKALRMPGVEMVITCFDVPNNFFATAGHPLSLDPNHADIKDKLILESRVRY
                     YGDDVAAVVADTPLHALLAAEQVQVEYEEYPPMMTPEEAIGNSCVLHDFRPNNELSRM
                     DFTIDINGNVRFYTADFSSENEIAGKADMIGDHYYVPAVHACHMENNGCFAYMEGTKM
                     IVVSCNQVPHTMRRNVADAINMPIGSVRVIKPFIGGGFGNKQDTMYEPLVALISKRLG
                     GRPVSLQLTREETFINTRTRHAFDMSMFTEVDDDGSILRKGLRINSNGGAYAAHAHAV
                     AAYAITNNFQTYMTKGEQIGESSTAYTTRPSAAAMRGYGIPQLAFAMESQMDDIALSH
                     GWDPIDFRMQNVMTDDFIDPFDKFVVKSNGARACMQKAREMSGWDEKRRAYTEFNLTS
                     KDIKKGIGMALFSYKTGVYPLQIENGACRIMMNEDGTATIQVGATELGQGSDTIFCQI
                     VSEITSIPESRLTVISTQDTDVTPYDNGAYASRQSYVSGGAVKKAAEILRDKLIDRAA
                     MILGVPAEELKLINEKIVTHDGREQMSIAMICKHMNFINDQHTLTEHITSEATYTAQN
                     ICFSYGVSVIDLEVDVPIGKVHINKVYSVHDSGQILNPQLAAAQLHGGVGMGIGYALG
                     EQMLFDEKTGRPLNNNFLDYKIPTSMDVPEIETAFIETYEPSGPFGNKGLAEPPLIPQ
                     APAIRNAILHATGVGVYALPMNPQNLVHAFRTAGLISD"
     gene            13953..14846
                     /gene="xdhB"
                     /locus_tag="PK898_03180"
     CDS             13953..14846
                     /gene="xdhB"
                     /locus_tag="PK898_03180"
                     /inference="COORDINATES: protein motif:HMM:NF007427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HOU19069.1"
                     /translation="MYDIQKYFSAHSIAEALDLLREYPGAIIVAGGTDIMVHLRQRKL
                     RDAVLIGIREIPELNGIHLHEDRSIVIGAGTCFDTIYQDTLINRYIPILAEAANQVGS
                     PQIRHVATIGGNLCNGAVSADSAPSLLAMDVKLEFRSKESTRILPISEFYIGPGKTAL
                     QSDHELLTAIIIPQENYENWNGCYLKFGQRNAMEISTLGCAAAVKLEKDKTHLVALNL
                     AFGVAAAKPVRCPRLESSVVGMAADDMLWQKIHTEVLSELNPRDSWRASRELREQLIR
                     TLSVRAVKKAIERAGGKNNGL"
     gene            14836..15306
                     /locus_tag="PK898_03185"
     CDS             14836..15306
                     /locus_tag="PK898_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU19070.1"
                     /translation="MGFKEINFILNGKPVTTTFSEGESLTDILRNLGMTSVKKGCEAG
                     ECGACTVLLDGKSINSCIYLADWVEGHDILTLEGVRAKDGSLHPIQQAFIDEAAIQCG
                     FCTPGLILTGVELCNSGKKYTREELRERVSGHLCRCTGYENIINALDKVIGVNS"
     gene            15315..15791
                     /locus_tag="PK898_03190"
     CDS             15315..15791
                     /locus_tag="PK898_03190"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOU19071.1"
                     /translation="MDHIAYLRKANEVAKQAVAEGNTPFGAILVDADGTILMEQGNRE
                     RELHDATAHAERMLASLASQTYDKAKLAECTLYTTVEPCPMCTGAIYWANIGCIVFGI
                     TEGKLLEMTGADEKNPTFNMGAAKVIQAGQKKINLLGPFPKVEEEIVALHLRFWKK"
     gene            16440..18227
                     /locus_tag="PK898_03195"
     CDS             16440..18227
                     /locus_tag="PK898_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU19072.1"
                     /translation="MKKLMTGNEALARGAYEAGVKVATGYPGTPSSEILENIVQYKEI
                     YAEWSTNEKVATEAAIGAAYAGQRSITTMKQVGMNVAADAFFFAVYTGMRAGLVIVVA
                     DDPGMFSSQNEQDNRHYAPFAKMPLLEPSDSQEAKDFVKIALEMSEEWDVPVLYRTCM
                     RVAHSASPVEIGERNWDDEKIPEKWVRDQDKYVCSAMLAKKKRPMIEDRLIKLVDVSN
                     KISINRLEWGDRKLGIIASGPVYHYAREIFPNATFLKLGMTYPLPEGLIRELAAGVDQ
                     VMVIEELDPFIEQQVKAMGINCIGKDIFPRYGELLTGDIERACAKAGIIPWEKVAEQR
                     DVTPENLPGRSPVLCPGCPHRSTFYNLAKMKDVIIVSDIGCYNLGALAPFYATDFMGS
                     MGAGFGVAHGFEIGGIEKTGQKIIGIVGDSTFFHNGVTPLLNMVHNGSNVTLMVLDNS
                     TTAMTGHQDHPGVDYTLRGKGTRVDIEKMVRAIGVEHVLKANAFDVKSVDSAIKEGLN
                     HDGLSFIIVEGPCFFVGNNLKPAYTVTSNCNGCGTCFKLGCSAIARSDIVDEKTGRRK
                     SSIDPVLCVGCGICAQVCPRKAIVSTQAS"
     gene            18247..18837
                     /locus_tag="PK898_03200"
     CDS             18247..18837
                     /locus_tag="PK898_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOU19073.1"
                     /translation="METINFLITGVGGQGTVLASEIMAAVGFAAGYDVKKSDLLGLSV
                     RGGAVLSHVRWGEIVYSPIVPEGRVDYLIAFEELEALRHLGQVKSTGTILMNQQKIFP
                     VLVSSGLAEYPTDEVIEESLRKTSKNVHEIPAVELALKVGNSKVLNIVLLGAISALFN
                     IDPKIWEEVIKERVPKKYVDLNIKAFNEGRNWMKGE"
     gene            18844..19551
                     /locus_tag="PK898_03205"
     CDS             18844..19551
                     /locus_tag="PK898_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOU19074.1"
                     /translation="MRICVINPNSSKSVTDHLRNTLNKIKRDDCELTVICPEHGPVSI
                     ESAYDETIAAFYVMDLVKKANEDGYDAIVLACFSDPGLDAAKEISSIPVIGIEEATLH
                     MAAMLGHKFSIMTGMERRIPTRELHITIHGLSQSYASSPALEMSVLEMDSNPQKAKEK
                     IIELARKAVKEDRAEVIILGCAGLAGYSEDIERELGVVVLDPSSVALKVAEAMVDLGL
                     HHSKVGRFSFPPAKEIK"
     gene            19594..20664
                     /locus_tag="PK898_03210"
     CDS             19594..20664
                     /locus_tag="PK898_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU19075.1"
                     /translation="MINLTRGVPPVEVFPIEELIKASEKNLREDGKRVLQYLHAPGYP
                     PLIEYLAKKHGVRKEEVVLGNSSLELLQFVTLTEMKPGDCAYIESPSYDRANTLLKRS
                     GADVVGIPLEIDGVNLDILEKELKKRPPKLFYIIPDFQNPMGTTTCAEKRKQIAKWAE
                     EYDFLVVEDAPYRELRYHGTAIPSIRSFAPDHVVTLSSFSKTLAPGLRTGYLYGREDI
                     VKRITAWEVDTYIGSVTPTQGQVYEYLNQGFFEPNLKKLCDLYRPRLDTLLNSLDKYL
                     PNAVYPRPEGGFFVGVTLPEGNSMEKLLPAAADAGFKLTDGRGFYLNPQDGDRFLRIP
                     FCSLSPEEISNAIQSLAKIIVH"
     gene            20718..22859
                     /locus_tag="PK898_03215"
     CDS             20718..22859
                     /locus_tag="PK898_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOU19076.1"
                     /translation="MTTSFRNLRPLLNPKSIAIVGASEKHGSAGRIAIENLHYLGYQG
                     EIFAINPKNESVLGHPCYKDLASVGKDIDMVAILIGAPQVKSVLEQMHSLNIPACWCL
                     ASGFSESGPEGKRLQDEIFKYCEENNILLCGPNCIGVANIPDKYAAFSVAIKPNIKAG
                     NISAVMQSGAILMGLANSARFGFRYLISAGNQAVLDISDYIGYLADDPETKVIIAFVE
                     GIPSSQKFIDAVRAANKAEKPVLMLKVGRSEGAQRAVQAHTGSLAGSDAVLDAILKKE
                     GVIRLNTLDELVEAAELFIASPLPDKDGVCILSLSGGQIGLIEDLSQGMGIHFPEFNQ
                     ETLAALHEILPDYSTISNPLDAWGNGDLEKMVPGCVSAVASQAEIGLIAMSRDTPRGV
                     AEREIEQSLRIAESAIKVKNENKKAVCLFSNFSGSFDPTVEQYLRESGIPYLQGTPEA
                     LRAIQALENYASFRKHPKFTAGSSPINLNDLKKWQNRFKQRNQEFSEIEGRKLLAAYG
                     IPGPKESVAATKEEAIAYASEIGFPVVMKINSPDIHHKTECGGVVVGIHNEQEVMKAF
                     DQIITSVRSYKPDAIIEGVIVQEMISSASVEVILGIINDKTYGPAIVYGSGGILVELM
                     KDSALAIPPLSREEASELVNKTQGSKLLKGFRGKPKADIDALLDSIVNLSYLAKDFEN
                     EIAAMDINPLMVLPEGKGVRAVDVLIERKMM"
     gene            23130..23642
                     /locus_tag="PK898_03220"
     CDS             23130..23642
                     /locus_tag="PK898_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOU19077.1"
                     /translation="MEQKGYKAVQCTRGREWILRISTGQDVYDGIQQFAKDNNIRFAQ
                     IHTAFMGGFEPARMLIWTPDSTNPDNWHHETPMDIQNLTMLLSMGGFIHLRENEEGKE
                     EPFPAIHYIVGAAWNAPIWGGHLVQGTIAKGNMEVFITEILGIDPIMEDNVNIGAPEN
                     WYIESNSSVK"
     gene            23656..24039
                     /locus_tag="PK898_03225"
     CDS             23656..24039
                     /locus_tag="PK898_03225"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU19078.1"
                     /translation="MSKREVIIGAKDLPFSKVIRFGELIFVSGMVGRNEDTHQIDFDI
                     KGQTKQLLQNMQKLLENCGSSLDNSIKITIYLTDMRYFSEMNEEYSKHFSTEKGFPTR
                     TCVAICSLPDKDAKIEMDVIAEVVK"
     gene            24036..24932
                     /locus_tag="PK898_03230"
     CDS             24036..24932
                     /locus_tag="PK898_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOU19079.1"
                     /translation="MKSKRVMVIDAFCDKPFQGNPCAVLPDARELSDEEMLLIAKEMN
                     LSETSFVLPSEKANFRVRYFTPRGELPFAGHPTIATSLMLAQENMIQITGNTTAISLE
                     FNIGVLPVEISSQNGKPNQAVMQLTAPVFGKTVSREKIAQCFKDIDPDDIRPDCPCQV
                     VGTGTNFLIIPLMTSSKLSNITMNRDNLMAIVDEVGVSAAYVFTKGGFSRETELSGRL
                     CDPRNAFEDPYTGSAVGAVGAYVIHYGLLTGDQIQVEQGHFIGRPGVGRLFIEHDGKE
                     IRSIKLGGSGVKVTDGFIFLEN"
     gene            24936..26090
                     /locus_tag="PK898_03235"
     CDS             24936..26090
                     /locus_tag="PK898_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOU19080.1"
                     /translation="MNETRINNLLKETNGVQGLVFKSPSHIGYLTNVLFPYPDQSPFN
                     IGLVTSAGKAVLIIPAEWKGVAEDQNWSDDILYYTINEGSPSEAFCKAINKTIIKLNL
                     NGKAIAVDYPSWTVAEINRANILFQNSQWSDCDKTIRQLRMKKDQAEVDLLHQAAVIA
                     DRGIIGALNHAEGTIGNAEYTLVEFLERVRVHAIEFGSSGIGHLNVSMGKQGRVWFTP
                     VRDFSLLENGNFLRVNYTQEFHGYWNLCNRTFFIGLPRYSDDSAYRLHQNLISLCLKE
                     LRAGIKISDFCSEVIQHAKALKVELFNENEFGHGIGLSEVEWPLLTIDNPESFQAGMV
                     IVLDIISLGPNEELIQSTETILIEENGYQRLSDFRDWDDLYCIVGCRTTH"
     gene            26101..27276
                     /locus_tag="PK898_03240"
     CDS             26101..27276
                     /locus_tag="PK898_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOU19081.1"
                     /translation="MPINKIKERISAILNENDDHLDALIITDDANLTYVTGVQLQLLA
                     SIPYLSVILFWSKNNRTLICPSALASTYTSQGWKENLIIYAECADPQGAAIAHLLQMI
                     QTEFKGRGNLGYIGESLSGKTLSQLTEALPNSNFMDISDSWIRLREIKTEEEIDNLSE
                     AAKLTDHGIAGAIHHLSTLQSKTEKFLTEDIRVHSLERGIHIGGYDAVSQAVPGEKAD
                     RLWPNAPAFGIGTGFTYNQDTFVRLEMLGIYNNYRSNDSRILYTGVLNQMQMDFFSKI
                     KDLRTYACSVINPGIAASKVYENILSKSKELGINVLNELGFGHGIGIEPVEPPYISAA
                     DDGIIAENMTLILKLAVRYLHGEILVTKDTLIVKPDGCQIVGRYQNWDIPYQTAYTF"
     gene            complement(27417..28124)
                     /locus_tag="PK898_03245"
     CDS             complement(27417..28124)
                     /locus_tag="PK898_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19082.1"
                     /translation="MILEVNDLHVNYGAIKAVKGISFYVDKGEIVTLIGANGAGKTST
                     LRAITGLEKVAQGSIHFKDTDITNMAPHKIVAMGITHVPEGRRIFTNMTVMENLRLAA
                     LLNKDPEITKAAYEKIFDLFPRLKERYMQSTLTLSGGEQQMLAVARAIVTGGELIVMD
                     EPSMGLAPKVVDEIFEVIQNLNQSGTTILLNEQNAYMALSIANRAYVLETGNITIEGP
                     AHELLNDPRVKEAYLGG"
     gene            complement(28108..28887)
                     /locus_tag="PK898_03250"
     CDS             complement(28108..28887)
                     /locus_tag="PK898_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19083.1"
                     /translation="MSVLKADHISIQFGGLRAVNNFSLEMNEKELFAIIGPNGAGKTT
                     VFNMLTGLYQPTEGAVYVNEKLMTNCKPYQFTEAGLARTFQNIRLFGQATVLDNVKMA
                     QTFRTNYSYYNMLTRTGNYYEEEKRITQRSMELLEMFDLQDKRNYFASNLPYGEQRKL
                     EIVRALATDPKVILLDEPAAGMNPNEIDDVITLIRRVREELGKAILIIEHHMKMVMSI
                     AEYIKVLDFGETISEGVPEVVQNDPKVIEAYLGVHVNDSRG"
     gene            complement(28884..>29387)
                     /locus_tag="PK898_03255"
     CDS             complement(28884..>29387)
                     /locus_tag="PK898_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU19084.1"
                     /translation="CRNFMDSSYGRACMAIRDNEIAADTMGINTAKYKIIAFVTSAFL
                     AGVAGSMYAHTLRYLHPDVFGYTKSTDFLVYLYAGGVGSISGSILGAFTLTALPEVLR
                     FMKEWRLVIYGGLLVVIILFRPVGMFGGNEFSFLKLKTGGIKSVGFSDLFKRKRHQEL
                     SNKEGIQ"
     assembly_gap    29388..29487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29488..29804)
                     /locus_tag="PK898_03260"
     CDS             complement(<29488..29804)
                     /locus_tag="PK898_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19085.1"
                     /translation="MVKTMKWLKRNSEVIIIPFIYILIAGLQRAGILNNYIIQVIMLG
                     GINIIMTESLNLVNGLTGQNSIGHAGFMAVGAYVSALFSKVIFNAGGLPPTSQTLVFL
                     LAT"
     gene            complement(29806..30708)
                     /locus_tag="PK898_03265"
     CDS             complement(29806..30708)
                     /locus_tag="PK898_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU19086.1"
                     /translation="MEKFIQLLVYGLQLGSIYALLAIGYTMVFGIIRMINMAHGDFLM
                     MGAYAAFFIVSLIFRDQMIGPAVALLVILASAGITGMIGVGVERVAYKPLRNRPKISS
                     MIAAIGVSLFTQNFFRAIPFIGPNPRTFPKLMKDVLFFIGPIQFSLVRIVVIILSVII
                     MAILYFFVMKTTMGMEMRAVSCDKDASSLMGIDVDRTISTTFFIGAGLAAISGAFYAN
                     SYPVLTVTMGATLGNKAFIAAVIGGIGDIRGAMLGGFIMGIIEIMISSVYSELAYGIS
                     FVFLIIILLMKPEGLLGKPMVEKV"
     gene            complement(30785..31942)
                     /locus_tag="PK898_03270"
     CDS             complement(30785..31942)
                     /locus_tag="PK898_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19087.1"
                     /translation="MKRILYVLLVFVMILGVGIATVSAEKEIPDVIKIGMISTISGEQ
                     ALDGQNMTDAIKLVQKELEASGGLDVDGKKVKIEFVIEDCEAKPEIAVNAAQKLIEQD
                     HVLAIIGPNNSGDTIASAEISQAAKIPQITNTGTNEAVTKVGDYIFRACFIDPFQGKV
                     VATFAYDNLKVTKAAAIYDNADTYSAGLDKAFVDAFEKLGGTVVANEAFSGAEVKDFS
                     AQLINIKNAKPEAVFVPAHISNIPLILQQIRGMGIDAILLGCDSWDYAALPDLVGKDV
                     IEGAYYVTGFSPEAETAAEFVKAFTELAGYRPSFCSAMAYEAAHIVLNAIQHAKTIDG
                     PGIRDAMMETDLDLPSGHVRFDENRNPIKAGVILQVKDGVAHYVTSVLPEE"
     gene            complement(32450..33379)
                     /locus_tag="PK898_03275"
     CDS             complement(32450..33379)
                     /locus_tag="PK898_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU19088.1"
                     /translation="MKLYLKTPLFPLTRINQKFHKNFQIKMECYQPSGSFKIRGISYV
                     CLDAKKKGISHLICASGGNAGYAVAYCARTLGMKCTIVIPKSASNYMAEVIRREGADV
                     IVHGDIFDDANEFALQITQEDKNAMFVHPFENPLLWEGHSTIVDELKEQTVKPDAIVL
                     SVGGGGLLNGVIQGIDRNGWSDVPVIACEVEGAPKFNLSLKAGKPIYLDEIHTLANTL
                     AGKTVSPESIEYAKNHNIISYLTGDANAARAVFEFIEDFRVIVELACGASMSVAYDEP
                     EIIRPYHHIVVVACGGSGVNLPLLLQYKQIFNI"
     gene            complement(33513..33965)
                     /locus_tag="PK898_03280"
     CDS             complement(33513..33965)
                     /locus_tag="PK898_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19089.1"
                     /translation="MRLSYQLDQISYEIGMINSFIEMVACGVKRLAISPPIDPSNLNI
                     MLEASEIISDGFGTKYRFEESLIVTDIQSAEFTEGKNSILYYATDDAIAEYLDLKEKI
                     KSLTEKNQYFGQKRREISFAFGRLLGYPDEVILRKIDSPSHIDPFVLI"
     gene            34167..34721
                     /locus_tag="PK898_03285"
     CDS             34167..34721
                     /locus_tag="PK898_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOU19090.1"
                     /translation="MFNQSDLPEWQRDATSPDQYHCCETRRGREFIVRMMTGSDPVLA
                     IQKFAKEQNIRFGKVHATFMGGFQPCHYYVWVPDIAHPDNWHLEGEAINTNLTMLCAI
                     GGMIGQRPTDDGGEEPFVAMHFVAGGAWDTGTIAGHLLEGTRVKGCMQVFVTELLDIE
                     VLKPVDVFGEAYTHPENFYKNVTK"
     gene            complement(34779..35654)
                     /locus_tag="PK898_03290"
     CDS             complement(34779..35654)
                     /locus_tag="PK898_03290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOU19091.1"
                     /translation="METIIRHGYPDECREVENVLREAFWDVYRPGCNEHYIYHQLRQS
                     SAYIPELDWIAETDQRIVGGIISSKAQVVDYNGLNHPVICLGPIGVLPAYQGLGIGKK
                     LISKSLEEAERTGFQAVVVLGDPAFYAQFGFEKASNYHIRASEAENPDNLLVKELRPK
                     GLEGVTGKVLFDPAFQVDEKAVNEFDRDFPAKQKKEAEPEVKESNLDDDQKWKRYNVI
                     IGISLGIILGFILFGIPQKYLDDGLRLTIMLVIILFFPQIFEKKFQVKFRLGRLWMAG
                     TLIFCTILFIFLKLI"
     gene            complement(35711..37351)
                     /locus_tag="PK898_03295"
     CDS             complement(35711..37351)
                     /locus_tag="PK898_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOU19092.1"
                     /translation="MIRYICKYTPLELFGSFGLKPEIFNAMLPDSDAADELTHRNMCG
                     FSRVLLADRIVNNTGVLVLTDCCDSIRRTADVLESAGQKIMRIALPHRQDERGQKLYK
                     EELLRIVQQLEELTETQFHPETFRSAFSREEQPSGPYIAVLGARIGNEILNEIQSVSP
                     YPIRNQTCTGNRRLGTPIGNNLDRLMEWYAHELLSQPACMRMTDIASRRSLTEDPDLR
                     GVIYNTVAFCDFYGFEYALLRGNLRVPLLKIETDYTIQGIGQLQTRLQAFFENVKPTR
                     KMNHTGKNQRMSGYYAGIDSGSTTTNAVIMDNKKQILASSTIPTGVQIMESANRALEE
                     ALLKADIKRKQISRTVTTGYGRAGISFREKEVTEITCHAKGAFFLNPAVRTVIDIGGQ
                     DSKVIRLDENGNVYDFVMNDKCAAGTGRFLELMSQSLGLTMEEMGTRGIDTKEDIVIS
                     SMCSVFAQSEVVSLIASGKRVEDIIHGLNRSIAAKVLSLAGRKVLEREWMMTGGVARN
                     AGVVNAVEEKLGGKVIVPDEPEICGAIGAALVAIDIDE"
     gene            complement(37348..38619)
                     /locus_tag="PK898_03300"
     CDS             complement(37348..38619)
                     /locus_tag="PK898_03300"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOU19093.1"
                     /translation="MNKIVSKFGGWVGSNLSKNPEKARQLLKIGFSVSLYQSKIFPDR
                     SLLPHQQFAYVMNTGAILKPFRNPDQAAIANLFFPCELLWAMDIDPFFTEGMAAYLNG
                     AACEHYFIRYAEDSGIPQTYCSYHKTLLGAVLANVIPVPRFAAYTTLACDANLNTFRS
                     IADYFHIPCFAVDVPEFESADTVAYVSDQLRDLANAVGHIMGKRLDEDRLKEIIRREN
                     HCVRMACEQLDLLADRFIPTDLTSEMNKIFPTHVNIGTENAEKYFRLLLEDTRNAKLS
                     EGEKRILWVHTIPYWQESIRNLFNQSRKYQILLSDMNYDFLEELDENAPYDALARKLI
                     RNPMNGRGQRRSEHILNLAKKLKADGVVYFCHWGCKQTLGNARLTSECLQKAGYPVLI
                     LDGDGCDHNNINDGQMITKLQAFLEMLETNR"
     gene            39393..40100
                     /locus_tag="PK898_03305"
     CDS             39393..40100
                     /locus_tag="PK898_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19094.1"
                     /translation="MIHIGDKRILLVAGHYGSGKTEFCINLAIDLAEQGIKTALVDLD
                     IVNPYFRTREKTQFLESKGIKVYGNFYKTEITMEIPALSAEVRTPLEDPTVYTIIDLG
                     GNSAGALVAIQFEKYFVEGTYEFLDVINKNRPETSTIEGAVNHFHDIEERTGLKFTGL
                     INNTHLLRETTIEDIQKGLEFCEELSNILKVPLLFTGCPNYLYEQLLSSKLMEKINHQ
                     ILPIHFIMRDTWLDKKL"
     gene            40118..40354
                     /locus_tag="PK898_03310"
     CDS             40118..40354
                     /locus_tag="PK898_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU19095.1"
                     /translation="MGKYLVKFNRDKCKGCELCVSFCPKKILEIDKVDMNAKGYHPAN
                     VKPGQMDECIGCLSCALMCPDNVISVYELSGDEK"
     gene            40351..41436
                     /gene="vorB"
                     /locus_tag="PK898_03315"
     CDS             40351..41436
                     /gene="vorB"
                     /locus_tag="PK898_03315"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU19096.1"
                     /translation="MTEKVLMKGNEAFCEAAIRGGCRFYVGYPITPQSEIPEFMSHEM
                     PKRGGTFIQAESELGSINIAYGIGCAGGNVFVSSASPGIALMQEGLSFLCSAEVPVVL
                     LNVSRSGPGIGGIQPAQADYNQVTRGGGNGDYKIPVLAPSTMQEAVNIIYESFETADK
                     LRNPVIILADGMMGQMMEPVTLPEPKQLYKDEADIPNYKPWALTGSKGKSHRNVIKSY
                     RALPEDLEVHVNHLFEKYRKAEKDLVRYETINTKDAEIVFVAFGTMARIVSEVIEVLN
                     AEGIKAGMIRPISLWPFPKAPFDELSPDKTKVVISTELNMGQMIFDVQLSLNGRIPVR
                     LINRVGGIVPTTDEIVERAKKILGEIK"
     gene            41433..42176
                     /locus_tag="PK898_03320"
     CDS             41433..42176
                     /locus_tag="PK898_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU19097.1"
                     /translation="MKPVYEPTIGMLPVDTSYCPGCGHGIAHKLIMECITEKGRLGDT
                     IGVSPIGCSVVAHEFMNIEMCESAHGRASAVATGIKRVHPNHLVFSYQGDGDMASIGV
                     TEIVHAAIRGEKITTFFINNAIFGMTGGQMAPTTLIGQKATTCIDGRKKEVNGMPIRM
                     SEMLATIEGAVYIERVALNTPGNVRKAKKAVMKALEVQEKGLGFSLVEFISSCPTNWG
                     LSPIDALKWVGDNMIPYYPLGVFRSPEGM"
     gene            42179..42712
                     /locus_tag="PK898_03325"
     CDS             42179..42712
                     /locus_tag="PK898_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU19098.1"
                     /translation="MAKEKLFFAGSGGQGILLMGKMLSYAAMHEGKEVTFLPSYGAEM
                     RGGTANCTVIISDKPISSPLIQTADALIAMNLPSMTKFENMVEPGKFLFVNSSIIKEK
                     PTRNDIQVVSVDANGLAETLGVPKSANMVMLGAVVRTTKVVKIESIIEAMEKELTGKK
                     AKFLEGNILALNSWNPN"
     gene            42734..>43809
                     /locus_tag="PK898_03330"
     CDS             42734..>43809
                     /locus_tag="PK898_03330"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOU19099.1"
                     /translation="MKHSFSITRSTNPKQKPNPDKLVFGETFTDHMFIMDYSKDKGWH
                     DGRIVPYGPISLEPAAAVFQYAIEMFEGMKAYRTDTGKVQLFRPDMNIKRTNNTNDRI
                     CVPRIDPDLMLDAIKAIVDFDRDWIPDRPGTSLYIRPFIIADEPYLGVHPAKHFLFII
                     ILSPVGSYYGVGGGSNQLPATKIYVEDKYARTAVGGTGAAKIGANYVISLKAQEEAAE
                     KGYDQVLWLDSAEKKYVQEIGTSNAFFVINNEVITAPLDDGTILPGITRDSCLALLRK
                     WGIPCSERPLTIEAVYQAAEDGTLNEVFASGTAAVISPVGEICWKGKKIPVKTGPIAQ
                     RLYDELTGIQTGKIPDTMGWTVEV"
BASE COUNT        12902 a         9188 c         9281 g        12338 t
ORIGIN      
        1 gagacaatcc ttccggtttc cggtataaaa gagaaaagat tagaggaata atcgatgtta
       61 aacaacgaat cactgacaga gctttccccc agtcaaaaaa tagatgctca aatcaatgaa
      121 ctcggtgagg attggcgagg tcaaaaattg aaagaaattc gtcaattgat caaacagaca
      181 gatcccgaaa tagttgaaga atggaaatgg agaggtacag ccgtatggtc gcacaatggg
      241 atcatctgta ccggtgaagt ctataaaaat gtcgtcaaaa tgacctttgc gaaaggagcg
      301 ctcttgccgg atcccgatca tttgttcaat tccggtttgg aaggaaatat cagaagagcg
      361 atcgatttcc atcaagatga tgaaataaac aaaatcgcac tttcacaatt aattcgggat
      421 gcgattactg taaacgattc ttcccgtaaa agcgttcaca aatccgaatc gatttaagct
      481 ccggtaaccg gaaaatgtcg tttagctttc gcttcaaaca tataattttt tctctgattt
      541 tttgccctat atctatcaat ccgtgaatat gatcgacaat cctttattta tcagccgata
      601 tatttaaatt tttcctgtca actccccaac aattgctgat cataaagaaa tagcacttca
      661 ttaatcaggt cgatatcata tagttgattt cgcataaaat aacttacaat cacatcaaac
      721 agctgatctc gcgataagtt aaagccggtt tgagccaaaa gttctaccgt ctcttcgaat
      781 ggaatcttta acgcaacaga cagtgcaata atcgtttgtt ttgtcacaaa agatccacct
      841 ttgcttccga ctttagaaaa ccaatcccgg ctgagattgg cctttgtaca aacctctatc
      901 tctgctaaac cgctcttttt aactatttgt gtaaggattt ccaaaaacgg ttccacattc
      961 ctctttactg aatcgccttc cccttcgttt gatattccac tcggcttttc atacacccga
     1021 tcgatatact cagataggtt ttttaatagg ttctcatcga ctacaatgga agatttgtcc
     1081 aaaacagcca aataaatctc catctcgttc tcagccaaaa aatcccgaat agcagaggtc
     1141 gctacactta atgcctcctt tttgggatag ccataaatcc cggcggatat gagcggaaat
     1201 gcgatggact tacatccgtt tttcaaagcc aatttcaggc tgttagagta acacgagcgg
     1261 agctgcatcg cctcaccatg ttttccgtcc cggtaaatcg gaccggcggt atggataata
     1321 tacttagcgg gaagtctgaa ccccggtgtg ataacggcat ctccggtttg aatcggagct
     1381 aatttatcac aagcagcttg tagctcagcc gctccggccg cccgaaaaat tgccccgcaa
     1441 acaccgcctc ccatccgaag agcggtattt gccgcattga cgattgcgtc tgtttttatt
     1501 tttgttatat cttgctgaat tatcagaaac ggcataattt tccttccttg atccgattga
     1561 ctctcatttt tttactgccg gctttatcga ttgattaact ggattccctc aaatcaaacc
     1621 gaaatatttc ctttctgata gcgaatgaat ccgtttctaa gataaaacaa tatctatgcg
     1681 taattcctat tctttcaatc cggtcctaaa agaatggtct tttaattata gtgaatgatc
     1741 ttctaccttt tcgaagcatt ttttggaaat tcctatttta tgtctggtac tatatataaa
     1801 ccgaattttt ctatgttttt ttgccggtcc gatatatctc ggtccttaat tcttatgtta
     1861 ttcattaaaa atagcgtctg gttgatattt ataaaccaag ttcatactcg attatttctg
     1921 agttgacaca gcgaataata ttgaaccaaa cagacaattt tttcgaatcg cgaagagaga
     1981 atcaattaac catatgctat ttatttagat gatttataga atcttattca aagctcaatt
     2041 tgtaataaat tctcgaacga tcggttaaag ccattaaaat ttacgaataa tcatcaatat
     2101 atctcgtttg actatatcgg aggctcaaaa aatgcaagat cgatcgcgta aaattgccgt
     2161 gacaggcgta ctgggtgccg ttacgattct tttggggtta actcattggg gatttattcc
     2221 ttggttcggc ggagtatccc tgaccattat gcacattccg gtcatcatcg gtgccattct
     2281 cgaaggaccg gctgtcggcg caggcatcgg tttaattttt ggattattca gcatgcttca
     2341 agctgccata gccccttccg gacctacgga tgtttggttc accaatcctc ttctttcgat
     2401 acttcccaga ttaatgatcg gaccgatcac gtggctggtt tatcattcac tgaaacattg
     2461 gcaagccggt gcaatcatca tcagtgccat cgccggatcg gcaaccaata cggtcctggt
     2521 tttagggatg atgggattgt tagggctgct cccatggcag gtgttgggtg gaatcgcact
     2581 gattaatggt ctgcccgaaa tcggcgcatc cgcactgatt tcattcttag taatcgtcgc
     2641 agtccgacaa atcaaagtcg gcaaaagaca aggttccgaa gtgtaaattc tccgccaatc
     2701 agaaaataat aattgaaaca acaatccata agacaatcaa aagaaaaacg gcttcacgcc
     2761 acccaaaaga atattccacc atgctggtgc gatttgcttt aaatccatat gctctggcat
     2821 ccattgcaag tgccatcgtt tccgctcggg ttaaactgat tgtaaaaagc ggtatcaaca
     2881 gtggaaagat ttgcttcaca cggctcaaga gtcccttgga tttaattccc cattccgctc
     2941 cgcgtgaagc ttgggctttg gcaattctct ccgcagattg agctaaaagg ggtagaaagt
     3001 gcaacgcgat ttggatcatc atgacaaaat ccatcgtatg aatccccaat gcgttcagcg
     3061 gatgaaggat gtgttgtaat ccatgaatca tttccgaagc agatacacaa aaagtcatca
     3121 gactgattgc caatatcaat gcactgaatc gaaaaatcaa cagcaatccc gacattaagc
     3181 tgagaatcga aacttgaatc ggcccgactc gaaacaacac ggactgacct cgaatcggga
     3241 aaaaagcaat ttgaatcaac gcaataacga tcaaaaaagg cagagggatc aatattcctt
     3301 ttagagcaaa acgcaaagga attttggcga ttattaatcc gataagagaa gcgattaatc
     3361 cgataccgac accgattatc gaaggactga atgtcatcgc taaaatcaag atcccgaatc
     3421 cgatcagctt cgaaccgggc tcaaggtgat gaaggataga tccggtcgga atgtattgtc
     3481 cgatcgtggt ttttgagaga aaatcaaagt cattcatttt tattaaaact cagtaaatcg
     3541 ttcacttcgg taaaaatttc acctaatgtt acagcgtcag atgaaatcgg ccaaccttta
     3601 tcccgtaaac attgtgcaaa acggacggaa atcggttgat ccaatcctac cgactccaat
     3661 ttctctcgat cggaaaacac ttgggaaacc ggtaaattgg atatgcttcg accttccgat
     3721 aggacagtca cattttctgc caattcgacg atatcgtcca tattgtgagt tgaaataatt
     3781 aactgcatct catttttttg cagcgatcgc agattctcta tcaaatttcg tcgggatcgc
     3841 ggatcaagtc cgcatgtcgg ctcatccata atcaaaagcg aaggatgaga ggccagagta
     3901 gccgccaaag ccactttccg ctgttcgcct ccgcttaaag tggcggtaat tcgatctttg
     3961 aaggtttcaa aatcaagccc cacaaaattc atagcccatt tcacgcgttc ccgtaaattt
     4021 tccgtcccgt aaaataattt cggaccgtaa gcaatttcgt ctcctacata ttgtttgaaa
     4081 aaataggatt ccggattttg gaagaccaat ccggcgtaat gtcgcaatgc ccgcagatcg
     4141 aaattcatgg aattcagatc aaacggaccg acttttacac gaccgctttg aggaagatac
     4201 agtccgttca aatgttgcaa aagactcgat tttcctgatc cggtcgcccc gataatacca
     4261 tgtggaaatc ctttgggaac agtcatcgaa acgtcgtaaa gagcctgctg ctgtaaaata
     4321 tttccggcta agtatgtata agaaagattt tgaatttcaa tttcatcgat ggccggttgc
     4381 gaaaaccgcg gagtgaattt gtctctttgc ctaaaagggc cggtgaagtc cggaatggct
     4441 ccaaacagat cctttagatc aatttttttg cccttaaaac cggtaaacca atcaggaaaa
     4501 gcttcgtaaa tacgatattc agtcggttgt tcgagcccac cggtatccaa caggttcgga
     4561 tcggaaaaaa ggacttcggg cgctccgtca aaaatcagag aaccttcatt tagaagaaga
     4621 actcgatttg cgagatttac ttcttccata atatgtgtga taaaaataat cgtaattccc
     4681 atccggttca aatcgcccat acactgaagt acatcccgac gaccgatcgg atccaacata
     4741 gcagttgttt catcgaaaat gatcgcgttc ggttgcatgg ctaacacacc ggccaatgcc
     4801 acccgttgca tttgtccggc tgaaagcata tgaggttgtc tgtaacgata gctttctgca
     4861 ttgacaacgg caagggatct attcacttgt tcgcgaattg tttttggaag acggcataag
     4921 ttttctggtc cgaaagcgac atcttcttca acaatcgtcg caacaatttg gtcttcgggg
     4981 tgttgaaaaa ccatcccgat ttcagatcgt attttcggaa cagcttgccg attacgtgta
     5041 ttttccccgt taatatacac attaccttcg gtcggtaaaa gcaaagcatt taactgacgg
     5101 gcaagagtcg ttttaccgga tccgtttgca cccaccagtg caatcaattc tcccgggtaa
     5161 atcgtaaagt ttatatcttt caagatcgga ggaaattctt cctctatccc cggttgtcgg
     5221 taagtaagat gatccgtctg gatcaaaggt ttcaacgcaa aaatcctgtt ccggctgact
     5281 gtcaaaaaat agccattcga attataccga cgatttatcc aaatcattca aaatctcgaa
     5341 agataaagga ttgagtctat gaaaataatc gataggaaat cggaaaataa ttattccttt
     5401 gaattgatag taattttttg taacaatggg ggcggacaag ggctgaaaag taaacccagc
     5461 ttttatctaa tataggtaca gaataacata tcagaagata agctgaaaaa atttaaaaat
     5521 agttttaatc aatcttcttt cttaatattg tatgagttat cattccttcg cgataatatt
     5581 cacttgaata aaggattggt ttccctaaca cattgtgacc gatctcatcc agatgtagga
     5641 gagcagttcc ctttgaaact tgcaattccg cagccagtaa tttatcggca ttcaccgcat
     5701 gaatttcggt taggtcaaaa aaaacatctt cttcgcaata tgtttttaaa aaatcgaaaa
     5761 tcggtttatg cagctcagat tcctcatagt tcatatttct tatcaaagaa gatgaaaaag
     5821 aatctataca aaaaatagtc gggacttcgt cagcactaat caatcttgtc acccggatca
     5881 tctgtgatcc tttttccaaa caaagtttct taatctccat agaatttgcc gttgaatatt
     5941 ctactttcga ataaactgtc ccaggtctat aacccgcatt ccgaataata tcgagaaatt
     6001 cctcttcaag atcaatacgc gtctgcactt caagaacctg tcgattgatg agcgttccga
     6061 tgccgtggat ccgatttata aaaccctccc gttctaatgt tgcaagcgca tcacgcattg
     6121 tcgaacgact tacaccaaaa tgctcagcta atctaatttc agggggaaga cgatcaagca
     6181 tacgatattc cccctcattt atttctatta aaagacgctg gactgtttgc gtatttactt
     6241 gtgtgctgaa cttattcatg acatttgtct cttatattga ttattctaac tgattttgcc
     6301 ttttacaaga cttgcaacat tgtcaaaaca aatatgacaa tattgatcca agcttattgc
     6361 tcccccgaaa aagatgttta ctctcatctg gagaattttt gatattgtct gacagtttgg
     6421 attttactat agacaattaa aataaaaagg tttattataa aaataggtcg taccaataaa
     6481 agacgagttt acaattcaaa ggagcaaaaa atgaaaaaat cgaatatgta tttaattgtc
     6541 attatggttc tcattgcaat tgtcacctgc ttaacaggtt ggcaaaaggc agatgcagcg
     6601 gataaactta aaatcgcttt ggttcttccc ggtaaaaaag atgatgtatc tttcaaccag
     6661 gccatgtacc gcggaatgat ggaatatgcc gaaaaacatg ccgatgaagt ggatgtcaaa
     6721 gttgtcgaaa acatctacga tgttgctgac attgaaccga ctttaattga atacgcaaat
     6781 gaaggtttcg atgttgtgat cggacatggg tatcagtttt cagaaccggt cattaccgtc
     6841 gcgaaaatgt atccggaagt cgctttcttg ctcggttgtg gtgttggcga tgcccccaac
     6901 gttattcgct atgatgtcca acttgaagcc ggtggttatc taatgggtgt tgttgctgct
     6961 ttggcgactc aatccaataa aatcggcgtc attggcggtg gtgaaggctc ggaaattacc
     7021 cgcggtcatg aaggatacaa agccggtgca agatccatca accctgatat tgagatccag
     7081 gaggtttata caggtgactg gaacgatacc accggcgctt atgaagcagc tattggcatg
     7141 tacgattccg gtgtagatgt aatctggcat tcgggtgacg gaatcggttt gggtgttgtt
     7201 caagctgccg aagaaaaaga taaatatgtc ctcggtaatg tcgaagatca gttttccctt
     7261 gcagagaaga atgttctttc aggccttcag tacgaatggg gtaatgcact tgaaaacatc
     7321 tttgcaatga ttaaagataa atcctttttc gttgagggaa aagacacggt tatgatcaac
     7381 gttgcaaacg gaggtctttc catgactgaa cttaacgatg tcaaaggttg gctgactgat
     7441 gaagacaaag caataattgc tgatgtttat gaaaaacttg gccagggtgc cattgagctt
     7501 cccgaagttt gatctatctt aatgaataaa acgaagggac ttgacctaac aagccccttc
     7561 gttaacttat aagccaggag gtaacacaat ggatgatcaa tgtgtcgttg aaatgcgcgg
     7621 aatcacaaaa attttcaatg gcgttaaggc aaatgacaat gtggatttct tcttaaaaaa
     7681 gagatcaatc catggtttat tgggagaaaa cggagccgga aaaacaactc tcatgaacat
     7741 cctatatggc ttgtatcagc aggaagaagg cgaaattttt ataaacggta aaaaagaaga
     7801 catttcatca ccgctaaaag caatctctat cggtatcggt atggttcacc aacattttat
     7861 gcttgccagg ccactgacag tgaccgagaa tgtaatgctt gggaagaaat caagacgtgg
     7921 tattctattg gatactaacc aaacatctaa cgagttgaaa gccctatccg ataaatatca
     7981 aatggatatc gatccgaatt ccaagatctg gcaactttct gtcggcgaac agcaacgtgt
     8041 tgagatttta accgctattt atatgggcgc tgagatccta attctggatg aacctaccgc
     8101 agttctgaca ccgcaagaaa ctgaatcctt ttttgaaacg cttcggcaga tgcggcaaga
     8161 cggaaaatca atcattctaa tcacccataa acttgaagag attatttcta tcgttgatga
     8221 agtaaccgtc ctccgcgacg gaaaaatggt cggacgaagg actatggacg caagtgttac
     8281 caaagacgat ttgtgccgaa tgatggtcgg tcgcaatgtg ctattcgatt ttccgcctgc
     8341 cgagacggag ccgggagaga taaaattctc aatccgcaat atttctgtta aaaatgacaa
     8401 aggcgtgcaa gcattgcaga atttctcatt ggatattcgc cggggtgaaa ttgttggttt
     8461 ggccggtgtt gatggaaacg gccaaaagga gctttgtgaa atccttaccg gacttcgtcc
     8521 tgcagaaagt ggtaccttac aactggatgg caaggacgta accaaccgaa ctccgaatta
     8581 ctacatcaaa cagcatattt cccatattcc tgaagaccgt caaacgatag gtcttgcgct
     8641 aaattggaat ctaaaaaata accttattat taaacaactc ggcaaaggtc cgtttactcg
     8701 caagggattt atagaagaaa aactggttga tcaaaactgg gagaaagcac gtgatgctta
     8761 tcagattaaa gctattgacg gtaacgaatt agctcgttcg ctttccggtg gtaaccaaca
     8821 gaaactgatt cttgctcgtg aattaagcct tgatccccat gtgctggttg caaatcaacc
     8881 tacccgtggc ttggatgtga gggcgtcaga atatgttcgc ggtcagattc ttaaagcgcg
     8941 aaatagcgga acagctgttt tgctagtttc tgctgatctc gaagaaattc tacaactatc
     9001 cgaccgtatc gcagtgatct atgatggtca gttgatggga atattgccgc gaggtgcaga
     9061 tccaatggat attggcgctc tgatgatggg gaagaaacaa gaggtacatg attgatgaat
     9121 gaaaaaacaa gaaaaacagc tattaatgcc ttcggtacaa tttgcatgat ccttattgcc
     9181 ttaatactgg gaggtatttt ggtatatttg acaggaaata gtccctttga ggcttatggt
     9241 gcgatgctgc gcggagcgtt cagtagtccg cagcgaatct ctgaactatt tgtcaaactg
     9301 attcccatct tattaatggc ttttggggta tccattgcat ataaagcgca actttggaat
     9361 attggtgcgg aagggcagtt catcatgtca tccattgccg caactgcagt cgcgctctat
     9421 attaatattc ctatcccggt acgttttatc ctctctttta tcgcatcttt ggcagccggg
     9481 gctctttacg caggattagc cggttacctt cgtaaccgct tcggggcaaa tgaagtgatc
     9541 acaaccatga tgcttaattc aattgcatcc tatttcttga tgtatctggt taatggtccg
     9601 atgcaggatc caaccacaga tttatctcaa tctcctcttg ttcctgaatc tatgtttttt
     9661 gctcgtctca ttggcaacta ccgtctacat atcggaatct tcatactgtt agcagtcaca
     9721 attatgatgt gctttttctg gaaatcttcc gtcggttatc gcatcaatct gatcggtcaa
     9781 ggtgaaatgg tcgcaacata tgcgggaatc aatgttaaac aaaccgttat gacaacgatg
     9841 atgatttcgg gtgcttttgc aggcttggct ggttggatag agacattcgg tatccaacat
     9901 cgaatccttg acggcattgc tgctgattac ggtaatattt ccactattat cgctctactt
     9961 ggctcgctaa atgtttacgg tatcatcgcc tcagcttgtt ttttttcggt actgctttgc
    10021 ggaggtgcaa gtatgcaacg aatgactgaa gtcccgtatt cggttgtcaa tgttatacag
    10081 ggcttgatta ttattcttgt gatcgcacgg aatatgtttg ccgacagact cccacaagtc
    10141 tcccataaag aaaagaaagg atagaaaaat gggaagcatt ttgaacatca cctttttcgt
    10201 cagcttattt gctggaatga tccgtctggc aaccccgatt ttgcttcccg cactaggaca
    10261 gatttatacc caacgcgccg gaatccttaa tctcggtgtt gaggggacaa tgttaatggg
    10321 tgcaatttgt ggttttatcg gagcttgcgt cactcagaat ctctggttcg ggtttctctg
    10381 cggtatttta gccggtatga tatacagttt gattatggct tggcttagta taaccatgcg
    10441 tgccaatcaa gttatcgccg gtatcggtat gaatattctc gcgagcggtc tggcttcgtt
    10501 tatctatcgt ctggtttttg gaatccgtgc cttacccgca aaaataactt ccttccagtc
    10561 tctgaaaata ccggtgcttg gagatatccc gtacatcgga acaattttct ttcagcataa
    10621 cattctggtt tatctgactt ttatccttgt tccaataact tggttcattc tcgaacgcac
    10681 gacttacggt ctgaaaataa aggcagtggg tgaaaatcca cgtgcggctg attcaaaagg
    10741 gattgatgta gctcgcattc gctattcggc tgtattggtc ggtggtgcgt atgccggagc
    10801 gggcggagct ttcatgacaa ttgcttatct caacacattt acagagaagg taatcggtgg
    10861 tttcggatat atcgccgttt ctgttgtaat ctttgccagt ttccaaccgg tacgggcaat
    10921 gtttggcgcc atgctcttcg gtctcgcctc tgctctgcaa ttgcgtctac aagcacacgg
    10981 tgtggggatt ccaagccaat tgttattgat gttgccatat ttgatgacaa ttatcgcatt
    11041 ggtaatagcc tccggaaaag ccgtctttcc gagcgcttat acgatacctt actcaagaat
    11101 ggaacgatga atcacagaat aacattgctt tttattacta ttatgcttat gactcaatca
    11161 gcttggtgct atgtagctcc gacagaagag cgtgcaatgg aacgagccca agcttatgcg
    11221 gaagttattg atcacagcta taagcatccc gagaagattt atgccatgct ggaaaaatct
    11281 ttccatgatc agatggacga aaaatccttc tgtaatgctt ttgcgaaaga acgtagctat
    11341 ccttatataa ctcctctcta tatcagcaaa cctcatctta ctttcgatgg gaatatcgcg
    11401 catgtcgtct atgatcaagc ggctcgcttg agcggaatga catataaaat cgacctgatt
    11461 tttgaaaatg gcgactggtt tgtccgagat tgggaggagt ttttggatgg cagctatctt
    11521 gaaaagttcg aaaatatacc ctactctctg gattggtatt atgacacaca gaaaatagaa
    11581 aaaggaaatt aaattatggg gattaaaaaa agtgtaacgc gaatagacgc attaatgaag
    11641 gttacaggac aagcaaaata tatcgaagac cttgttccaa gagatgcgct acacgtaaaa
    11701 attgtgcata gcacgatcgc taacggtgtg gttaaaaaaa tcgacagtga aaaagcgttg
    11761 cggatgcccg gagttgagat ggtcattaca tgctttgatg ttcccaataa ttttttcgca
    11821 actgctgggc atccgctgtc attggatccg aatcatgccg atattaaaga taaattgatt
    11881 ttggagagtc gcgttcgcta ctatggtgac gatgtggctg cagttgtcgc tgatacgccg
    11941 cttcatgctc tgttggctgc ggaacaggtt caggtagaat atgaggaata tcctcctatg
    12001 atgacccccg aagaagctat cggaaactca tgtgtactgc atgatttccg tccgaataac
    12061 gaattgtcac gtatggattt cacgatagac atcaatggga atgtccgttt ttataccgct
    12121 gatttctcct ctgagaacga aatcgccgga aaggcggata tgatcggaga tcattactat
    12181 gttccggctg ttcatgcctg ccatatggaa aataacggct gttttgccta tatggaaggt
    12241 acaaaaatga tagtggtttc ctgcaatcag gtccctcata ctatgcgtcg caatgtagca
    12301 gatgccatca atatgcccat cggctccgtg agagtaatca aaccttttat cggtggtggc
    12361 ttcggaaaca aacaagatac catgtatgaa ccattggtgg ctttgattag caaacgtctg
    12421 ggtggtcgtc ccgtttccct tcagttgact cgcgaggaga cctttataaa cacacgtact
    12481 cgtcatgcct tcgatatgag catgtttaca gaagttgatg atgacggttc aatcttgcga
    12541 aaagggcttc gtattaattc taacggcggc gcctatgcag cccatgctca tgccgttgcg
    12601 gcttatgcca ttaccaataa tttccaaaca tacatgacaa aaggtgaaca aatcggggaa
    12661 tcttccactg cttatacgac ccgtccctcc gctgcagcca tgcgaggcta tggtattcct
    12721 cagctagcgt ttgctatgga aagtcaaatg gacgatatag cgctttccca tggctgggat
    12781 ccaatcgact tccgcatgca aaacgtcatg actgatgatt tcatcgatcc ttttgataaa
    12841 tttgtggtta aatcaaacgg tgctcgtgcc tgtatgcaga aagcacggga aatgtccggc
    12901 tgggacgaga aacgccgcgc ttatacagag ttcaatctta cttcaaagga catcaaaaaa
    12961 ggtatcggaa tggcattgtt ctcctacaag actggcgtct accctctgca aattgaaaat
    13021 ggagcctgcc gaattatgat gaacgaagat ggaacagcca ctatccaggt tggagcaacc
    13081 gaattggggc agggatccga tacaattttc tgccaaatag tttctgaaat caccagtatt
    13141 ccggagtctc gtctgacagt gatctctaca caagataccg acgttactcc ttatgacaat
    13201 ggtgcttatg cttctcgcca atcttatgtt tccggaggcg cagtaaaaaa agctgcagaa
    13261 attttaagag acaaattgat cgatcgagct gcaatgatat tgggagttcc tgccgaagaa
    13321 ttaaagctga ttaatgaaaa gatagtcaca cacgatggca gagaacaaat gagcatcgca
    13381 atgatctgca agcatatgaa ctttattaat gatcaacata ctcttactga acatataacc
    13441 agtgaagcaa cctatacagc acaaaatatc tgtttttctt atggtgtcag cgttatcgat
    13501 cttgaagttg atgtaccgat cggaaaagtc cacatcaata aagtctactc cgttcatgat
    13561 agcggacaga tactgaatcc tcagttagca gctgctcagt tacatggcgg agtgggtatg
    13621 ggtatcggat atgcgcttgg agaacaaatg ttattcgatg aaaaaaccgg tcgcccatta
    13681 aataacaatt ttcttgacta taaaatacca accagtatgg atgtcccgga aattgaaacc
    13741 gcttttatcg agacctacga accgtcaggg ccctttggta acaaaggtct agcggaacca
    13801 ccattaatcc cacaagcgcc ggcgatccga aacgcaatcc tccatgctac cggcgttggg
    13861 gtatatgctt tgccaatgaa tcctcagaac ctggtgcatg cttttcgtac agcaggttta
    13921 ataagcgatt gatgtaaagg ataagtctat ctatgtatga tattcaaaaa tatttttctg
    13981 ctcacagcat cgctgaagca ttggatctcc taagagaata tccgggagcg ataatcgttg
    14041 ccggaggcac tgatattatg gtccacttac gtcagcgtaa gttacgcgac gctgtgctca
    14101 tcggtatccg tgagatccct gaattaaatg gtattcatct gcatgaagat cgctctattg
    14161 tcattggtgc cggtacgtgt ttcgatacaa tttaccagga tacattgatt aataggtata
    14221 ttccgatttt agctgaagct gccaatcagg tcggtagccc tcagatccgt catgttgcta
    14281 cgatcggcgg caatctttgc aacggagctg ttagtgctga cagcgctcca tctttattgg
    14341 cgatggatgt taaacttgaa tttcgttcta aagaaagcac aaggatcctt ccgatatcgg
    14401 aattttatat tggtccggga aagaccgcct tgcaatcaga tcatgaatta ctaaccgcca
    14461 tcattattcc acaggaaaac tatgaaaatt ggaatggttg ctatcttaaa ttcgggcaac
    14521 gcaatgccat ggaaatttcg acactcggtt gcgcagcagc cgtaaaactt gaaaaagaca
    14581 aaactcacct tgttgcatta aatctggcct ttggcgtggc agctgcaaaa cccgtacgct
    14641 gtccccgtct tgaatcatct gttgtcggaa tggctgctga tgatatgctt tggcaaaaga
    14701 tccatacaga agtcctttct gaacttaacc cgcgggacag ttggagagca tccagagaat
    14761 tacgcgaaca actgattcga acactgagtg tgcgtgcagt caagaaagcg attgagagag
    14821 ccggaggtaa aaacaatggg ctttaaagaa ataaatttta ttttgaatgg gaaacctgtc
    14881 accacaacat tctctgaggg agaaagctta accgatattc tgcgtaatct tggtatgaca
    14941 agtgtcaaaa aaggctgtga agccggtgaa tgtggagcat gcacagtctt actcgatgga
    15001 aaatcgatca acagttgtat ctacttggca gattgggttg aaggtcatga tatcctcacc
    15061 cttgaaggcg ttcgggcaaa agatggatct ttgcatccga tacaacaagc ttttattgat
    15121 gaggcggcca ttcagtgtgg tttctgcaca ccgggtttaa ttctgacggg tgtggagctt
    15181 tgcaatagcg ggaaaaaata tacccgggaa gaattacgtg aacgggtttc cggacatcta
    15241 tgccgttgca cgggttacga aaatatcatt aatgcattgg ataaagttat cggagtcaat
    15301 tcgtgaggga aaaaatggat catatcgctt atcttcgaaa agctaatgaa gttgccaaac
    15361 aagccgttgc tgagggtaat acgcctttcg gtgccatttt agtcgatgct gacggcacaa
    15421 ttttaatgga acagggaaac agggaacgag aattgcatga cgcaacagct catgctgaac
    15481 gtatgctggc ctcactcgca agccagacgt atgataaagc caaacttgct gaatgtacct
    15541 tatatacaac tgtcgaacct tgcccaatgt gtacaggtgc aatttattgg gctaatatcg
    15601 gttgtatcgt cttcggaatt accgaaggaa aattattgga aatgaccggt gccgatgaga
    15661 agaatccgac tttcaatatg ggtgcagcaa aagtgataca ggcgggacag aaaaaaataa
    15721 atttgcttgg acctttccca aaagttgaag aggaaatcgt tgcacttcac ctcaggtttt
    15781 ggaaaaaata attggtagaa agcaataaga caactgatct caaaattcgt tttggaatgc
    15841 aatttttaaa ttacaaacga acggtaaaaa tattatctgc cttcagataa tttcatttcg
    15901 gtatgatgca aacaccgatg atatccgatc gaagattatc tctatattat tctcaaaaga
    15961 tctttgtaaa acttgtttat tcatcagctt gtgattcaaa atttgaatct ttccaacttt
    16021 gaaaataaga cattttgaaa tggctgctat cagaacaagt gtaaatagcc ttgtcaatcc
    16081 tcaagttcat ccttgacaga attgaagaac catttagaat tagaaaggat tccaaaatta
    16141 gagaaagaaa aattatccaa aaatgtttca tttacaggaa aacttctctc gaaataattc
    16201 taaaattgaa aattctgaaa gttttttttc agaactcctt ttattcaact ttttacaatc
    16261 cgttagttta cccactgttc aaataatccg aatacgtctc aagcagtaac accttctgct
    16321 gaggacaatc tatgcatagc atagattaat atgtgaccaa gatttttctt ggttattttg
    16381 tttatgccaa tagattcaca gtttttattg atttgagtat tgaggattaa ggagaaaata
    16441 tgaaaaaatt gatgacaggg aacgaagctc ttgcgcgagg cgcttacgag gcaggagtaa
    16501 aagttgccac cggataccca gggacaccca gctcggaaat cttggaaaat atcgttcagt
    16561 ataaagaaat ttatgccgaa tggtctacaa atgaaaaagt tgccacggaa gcagccatcg
    16621 gagctgccta tgccggccag cgttccatta caacgatgaa acaggtcggc atgaatgtcg
    16681 ctgccgatgc attcttcttt gccgtctata caggcatgcg agccggcttg gtaatcgttg
    16741 tagcagacga tccgggcatg ttcagttctc aaaacgagca ggataacagg cactatgcac
    16801 cttttgctaa aatgcctttg cttgaaccat ccgacagtca agaagcaaaa gattttgtaa
    16861 aaattgctct tgaaatgtca gaagaatggg atgtgccggt tttatatcgc acatgtatgc
    16921 gcgttgccca ttccgcttca ccggttgaaa tcggggaaag aaattgggat gatgaaaaaa
    16981 ttcccgaaaa atgggtcaga gatcaggata aatatgtttg ttcggcaatg ttggcaaaga
    17041 aaaaacgacc catgattgaa gatcgcctga taaagttggt ggatgtttcc aataaaattt
    17101 ccattaaccg tttggaatgg ggagatcgaa aattgggaat aatcgcatcc ggtccggttt
    17161 atcattatgc gcgtgaaata tttccgaatg cgacttttct aaagctcgga atgacctacc
    17221 cccttcccga aggtttaatc cgtgaattag cggcgggagt cgatcaggtt atggtcatcg
    17281 aagaattgga tccctttatc gaacaacagg taaaagcaat ggggatcaat tgcatcggaa
    17341 aggatatatt cccccgatac ggagaattat tgaccggcga tattgaaaga gcctgtgcga
    17401 aagcgggaat tattccttgg gagaaagtcg ctgaacaacg agatgtaact cccgaaaatc
    17461 ttcccggaag atctccggtt ttatgccccg gttgtcctca tcgaagtaca ttttataacc
    17521 tcgccaaaat gaaggatgtc atcattgtca gcgacatcgg ctgttataac ttgggcgctt
    17581 tagctccctt ttacgctacc gattttatgg gatcaatggg cgccggtttt ggcgttgcac
    17641 atggtttcga aatcggcgga attgagaaaa ccggccaaaa aattatcggt atagtcgggg
    17701 attcgacttt ctttcacaat ggcgtgactc cgttgctgaa catggttcac aacggaagca
    17761 atgtcacatt gatggtattg gataattcaa ccactgcaat gaccggacat caagatcatc
    17821 ccggtgtcga ctacacactt aggggaaaag gaacacgggt agatatcgaa aaaatggtca
    17881 gagcaatcgg agttgagcac gtccttaaag caaatgcttt tgacgtgaaa agtgtggatt
    17941 cggcaataaa agaaggctta aatcatgatg gtttatcctt tatcatcgtc gaaggcccct
    18001 gcttctttgt cggaaataat ctgaaacccg catatacagt gacttcaaat tgcaatggct
    18061 gcggtacctg tttcaaatta ggttgttctg cgatagcccg atcggatatc gtggatgaaa
    18121 aaacggggag aagaaaatca tctattgatc ctgtcctttg tgtcggatgc ggaatctgcg
    18181 ctcaagtttg tccgcggaaa gcaattgtat caacccaagc ctcataacca gacaaggaaa
    18241 aactttatgg aaacgatcaa ttttttaatt acaggagtag gcggacaggg tactgtctta
    18301 gcaagtgaaa ttatggctgc ggtcggtttc gcagcaggat atgacgtaaa aaaatccgat
    18361 ctgcttggac tttcggttcg gggaggcgcg gtactcagcc atgttcgatg gggtgaaatt
    18421 gtttactcgc cgatcgtacc ggagggacgg gtggattatc tgattgcatt cgaagagctg
    18481 gaagcgctcc gccatctcgg tcaagtaaaa tcaaccggta caatcctgat gaatcagcaa
    18541 aaaatattcc ctgtgttggt ttcaagcgga cttgccgaat atccgaccga tgaggtcatc
    18601 gaagaatctc tgcgtaagac ttcgaaaaat gttcatgaaa ttccggcagt tgagttggca
    18661 ttgaaagtcg gaaattcaaa agtattgaat attgttcttc tcggagcaat atccgcactt
    18721 tttaatatcg accctaagat atgggaagaa gtcataaaag aacgggtccc taaaaaatac
    18781 gttgatttaa atataaaagc ttttaatgaa ggtcgcaatt ggatgaaagg agaataaaca
    18841 accatgcgaa tttgtgtaat taatcccaat tcttctaaga gtgtgacgga tcaccttaga
    18901 aataccttaa ataagatcaa aagagatgac tgtgaactca ccgtgatctg ccctgaacat
    18961 ggacctgtct caattgaatc cgcttatgat gagacaatcg cagcttttta tgttatggat
    19021 ttagttaaaa aagcgaatga agatggatat gatgccatcg ttctcgcctg cttttcagat
    19081 cccggtttgg atgccgcaaa agaaatttct tcaatccctg tgatcggtat tgaagaagca
    19141 accctgcata tggcagcgat gctcggtcat aaattttcga ttatgaccgg aatggaaaga
    19201 cggattccga cccgcgaatt gcatatcact atacatggtt tgtctcaatc ttatgcctct
    19261 tctcctgctt tggaaatgtc ggttcttgaa atggatagca atccccaaaa agcaaaggag
    19321 aaaatcattg aattagcccg taaggcggta aaagaagatc gggcagaggt gataattctc
    19381 ggttgtgccg gattggctgg atattcggaa gatatcgaac gtgaattagg agtggttgtt
    19441 ctcgatccga gttctgtcgc acttaaagtt gcggaagcaa tggtagatct tgggttgcat
    19501 catagcaaag tcggacgctt ttcatttcct ccggctaaag aaataaaata aataattttt
    19561 atagatttaa atcttattga cagaaaggat tatatgatca atttaactcg tggcgttcct
    19621 ccggttgaag tattcccgat tgaagaactg ataaaagcat ccgaaaaaaa tctgagagaa
    19681 gacggaaaac gtgttctcca atatctgcat gcgcccggct atccacccct gattgagtat
    19741 ttggcaaaaa aacatggagt aaggaaagaa gaggttgtct taggaaacag ttcgttagag
    19801 ttgctccaat tcgtgacact caccgaaatg aaaccgggag attgcgccta tatcgaatca
    19861 ccttcttatg accgagccaa tacacttctg aaacgctcag gggccgatgt tgtcggaata
    19921 ccgcttgaaa tcgatggagt taatctggac attttagaaa aggaactgaa aaaacgcccc
    19981 ccaaagttat tctatatcat tcccgatttt caaaatccga tggggacgac tacctgtgcg
    20041 gaaaaaagga agcaaattgc caaatgggcg gaagaatatg attttctcgt cgttgaggat
    20101 gcaccttacc gtgagttgcg ttatcatggg acggcaattc cttccatccg gtctttcgct
    20161 ccggatcacg ttgtgaccct ttcttcgttt tcgaaaacgc tcgcaccggg gcttcggacc
    20221 ggatatttat atgggcgaga agatattgtg aaacgaatca cggcatggga ggttgacact
    20281 tatatcggtt cggttactcc gacacaggga caggtttatg aatatctgaa tcaaggattc
    20341 tttgaaccaa acttaaaaaa attgtgtgat ttatatcgcc cgcgtttaga tactttactg
    20401 aattcgctgg ataagtatct tccgaacgcc gtgtaccccc gtcccgaagg gggattcttt
    20461 gtcggtgtca cacttccgga aggaaattca atggagaaat tgttaccagc agccgctgat
    20521 gccgggttta aattaaccga cggccgcggt ttttatttga atccgcagga tggggatcga
    20581 ttcttaagaa ttcccttctg cagcctttct ccggaagaaa taagcaatgc catccaaagt
    20641 ttggcaaaga tcatcgtcca ttaatttata aaatgaatca aggggaagag gattcttccc
    20701 cttgattagg aaaaattatg accacttctt ttcgcaacct acgcccttta ttgaatccca
    20761 aaagcatagc cattgtcgga gcttccgaaa aacacggaag tgccggacga attgcaatcg
    20821 aaaatttaca ctatttgggt tatcaaggtg aaatttttgc aattaatccg aaaaatgagt
    20881 ctgtattggg acacccgtgt tataaggatc tcgcatccgt cgggaaagat atcgatatgg
    20941 tagcaattct gatcggtgcc ccacaagtaa aatctgtttt ggaacagatg cattcgctga
    21001 atatcccggc atgctggtgc ctggcaagcg gattttcgga atccgggccc gaaggcaaga
    21061 gactccaaga tgaaattttc aaatattgtg aggaaaacaa tattttgctt tgcggaccaa
    21121 actgcatcgg cgtcgcgaat attccggata aatatgcggc ttttagtgtg gcgattaaac
    21181 caaatatcaa agccggaaat atttccgccg tcatgcaaag cggggcaatt ttgatgggat
    21241 tggcaaattc ggccagattt ggatttcgtt acctgatttc tgccgggaat caagcggttt
    21301 tagatatttc ggattacatc ggttatttgg cagatgaccc cgaaacaaaa gtaatcatcg
    21361 catttgtcga agggattcct tcttcacaaa aatttatcga tgcggttcga gccgcaaata
    21421 aagccgaaaa accggtttta atgctgaaag tcggacgttc ggaaggcgct caacgtgctg
    21481 ttcaggcgca tacaggatct ctggccggat ccgatgcggt tttggatgcg attttgaaaa
    21541 aagaaggcgt gatccgtttg aatacgttgg atgaacttgt cgaagccgca gaattattta
    21601 ttgcttcacc gctgccggat aaagacggcg tttgcattct tagtttatcc ggtggacaaa
    21661 tcggcctcat tgaggatctt tcccagggaa tgggaatcca ttttccggaa tttaaccaag
    21721 aaaccttggc cgcccttcat gagattttac ccgattacag tacgatctcc aatccgctgg
    21781 atgcatgggg aaacggggat ttggaaaaaa tggtccccgg ttgtgtttcc gccgtagcat
    21841 cccaagcaga aatcggcttg atcgcaatgt ctcgggatac cccgcgcggt gttgccgaac
    21901 gggaaatcga acaatcctta agaattgccg aatccgccat taaagtgaaa aacgagaata
    21961 aaaaagcggt ctgcttattc tcaaatttct caggtagttt tgatccgacg gttgaacaat
    22021 atttaaggga atcaggtata ccgtaccttc aggggacacc ggaagcactt cgggcaattc
    22081 aggctcttga gaattatgca tcttttcgca aacatccaaa attcacagcg ggaagcagcc
    22141 cgattaattt gaatgatctg aaaaaatggc aaaatcgctt caaacaaagg aatcaagaat
    22201 tttcagagat cgaagggcgt aaattattgg cggcatatgg aataccggga ccgaaagaat
    22261 cagtcgcggc aacaaaggaa gaagcaatag cttatgcatc cgagatcgga ttcccggtag
    22321 taatgaaaat taactctccc gatattcatc ataaaacgga atgcggagga gttgtggtcg
    22381 gtatccataa tgagcaggag gtcatgaagg ctttcgacca aattattacc tccgttcgat
    22441 cttataaacc ggatgcaata atcgaaggcg taatcgttca agaaatgatt tcatccgcat
    22501 ctgttgaggt aattttggga atcatcaacg ataaaactta cggtcctgca attgtatacg
    22561 gatcaggcgg tattttggtt gaattaatga aggattcggc gttggctata ccgcctcttt
    22621 caagagaaga agcttcggaa cttgtgaata agactcaagg aagtaagcta ttgaaaggtt
    22681 tccgcggaaa acccaaggcc gatatcgatg cattactgga ttcgattgta aatctatcct
    22741 atcttgccaa agattttgaa aacgaaattg ccgcgatgga tatcaatcca ttaatggttc
    22801 tcccggaagg aaaaggcgta cgagcagtag atgtattgat cgaaaggaaa atgatgtagg
    22861 gcttatttct tgagagagtt tgacgaatat caggtcattt atcattttat tcatctcttt
    22921 atcccctgct tttcgtcatc tctctcgctt tattatcgga tcgattagca aaatgaggca
    22981 atcgtttttc agatcggacc tgaaaacatg ctcatggcat caataggatt ggccggaaat
    23041 aaagaatatc aaattactga atcgtttctt taaaatatta caaaaagaaa aaatgtattt
    23101 catatcaatt gataaaaatg aggcatcata tggaacaaaa aggttataaa gcagttcaat
    23161 gtactcgcgg gcgagaatgg atattacgga tcagtaccgg tcaggatgtt tatgacggaa
    23221 tacagcaatt tgcaaaagac aataatatcc gatttgccca aattcatacc gcttttatgg
    23281 gcggattcga accggcgcga atgctgattt ggacacccga ttcgacaaat cccgataatt
    23341 ggcaccacga gacaccgatg gatattcaaa atcttacaat gcttctcagt atgggaggtt
    23401 ttatccattt acgggagaat gaagagggga aagaagagcc gtttcctgcg atccattata
    23461 ttgtcggagc ggcatggaat gctccgattt ggggcggaca tctggtccaa ggtacgattg
    23521 caaaagggaa tatggaagtc tttattaccg aaattctcgg tatcgatccg attatggagg
    23581 ataatgtcaa tatcggagcg cccgaaaatt ggtatatcga atcaaattct tccgtaaaat
    23641 agcagaaaag ggaatatgag caaacgagaa gtcattatcg gggcaaaaga tttgcctttt
    23701 tcaaaagtta tccgtttcgg agagttgatt tttgtttccg gaatggtcgg gcggaatgag
    23761 gatactcatc aaattgattt cgatataaaa ggacaaacca agcaattatt acaaaatatg
    23821 caaaaattgt tggaaaattg cggatcttct ttggacaatt cgataaaaat aacgatctat
    23881 ttaaccgata tgcggtattt ctccgaaatg aatgaagaat attcaaaaca cttttcaacc
    23941 gaaaaaggct ttcctacgcg cacttgcgtg gcgatatgct cacttccgga taaagacgcc
    24001 aagattgaaa tggatgtgat tgcggaggta gtgaaatgaa atcgaaaaga gtaatggtca
    24061 tcgatgcatt ttgtgacaaa ccttttcagg gaaacccctg cgcggttttg cccgatgcaa
    24121 gggaattgtc ggatgaagaa atgcttctga tcgcaaaaga aatgaacctc tccgaaacaa
    24181 gttttgtctt accttccgaa aaagcaaatt ttcgagtccg ctatttcact ccgagaggag
    24241 aactcccatt tgcgggacat ccgacgatcg caacctcatt gatgctcgcg caagagaata
    24301 tgattcaaat tacaggaaat acgacagcaa tttctttaga gtttaatatc ggtgttctcc
    24361 ctgtggaaat ttcatcccaa aacgggaaac cgaatcaagc cgtcatgcaa cttaccgcac
    24421 cggttttcgg aaaaaccgtc tcaagagaaa aaatcgcgca atgctttaaa gatatcgatc
    24481 cggatgacat ccggccggat tgtccctgtc aagttgtcgg aaccggaaca aatttcctga
    24541 taattcctct gatgacatct tcgaagctct cgaatattac gatgaatcgc gataatttaa
    24601 tggcgatcgt tgatgaagtg ggagtcagcg ccgcatatgt ttttacgaaa ggcgggtttt
    24661 cccgagaaac cgaattaagc gggcgattgt gcgatccgag aaatgctttt gaagatccgt
    24721 ataccggttc cgctgtcggt gcggtcggtg catatgtaat tcactatggg ttattaacgg
    24781 gtgaccaaat tcaagtcgag caaggccatt ttatcggacg accgggagtc ggcaggcttt
    24841 ttattgaaca tgacggaaaa gaaattcgat ctattaaact cggcgggagc ggagtaaaag
    24901 ttaccgatgg attcattttt ttggaaaatt aagctatgaa cgaaacgcga atcaataatc
    24961 tattaaaaga aacaaatggt gtacaagggc ttgttttcaa atcgccttcc catatcgggt
    25021 atttgactaa tgttttattc ccctatccgg atcaatctcc tttcaacatc ggattggtca
    25081 cttccgcagg caaagcggta ttaattattc cggcagaatg gaaaggcgtt gcggaagatc
    25141 aaaattggtc ggatgatatc ttgtattaca caattaacga aggttctcca tccgaggctt
    25201 tttgtaaagc aataaacaag acaatcatca aactgaattt gaacggaaaa gcaatcgccg
    25261 tggattaccc gtcttggaca gtcgctgaaa tcaatcgggc aaatattctt tttcagaatt
    25321 cacaatggtc tgattgtgat aaaaccattc gccaattacg tatgaaaaaa gatcaggcgg
    25381 aagttgattt actccatcaa gccgctgtga ttgccgatcg gggcatcatc ggtgcattaa
    25441 atcatgccga aggaactatc ggaaatgcag aatatacctt ggttgaattt ttggaaaggg
    25501 ttcgtgttca cgctatcgag ttcggttcgt caggcatcgg ccacctgaat gtttcaatgg
    25561 gcaagcaagg acgtgtttgg tttacccctg tccgtgattt ttccttgtta gaaaacggaa
    25621 atttcttgcg tgtaaactac acgcaagaat ttcatggcta ttggaatctt tgcaatcgca
    25681 cattttttat cggactcccc cgctattccg atgattcggc ataccggtta catcaaaact
    25741 taatttcgct ctgcttaaaa gagctacgtg ccgggataaa aatcagtgat ttttgttctg
    25801 aagttattca acacgcaaaa gctttgaagg tcgaattatt taatgaaaac gaattcggtc
    25861 atggaatcgg actttccgaa gtggagtggc cgttgctcac aatagataac ccagaatcat
    25921 tccaagccgg tatggtaatc gtattggata tcatttcatt gggaccaaat gaagaattaa
    25981 ttcaatcgac ggaaacaata ttaattgaag aaaacggtta tcagagattg agtgattttc
    26041 gtgattggga tgacctttat tgtatcgttg gctgccggac aacccattaa ggagcgtatt
    26101 atgccgataa ataaaatcaa agagagaatt tcagcaatat taaatgaaaa cgatgatcat
    26161 ttggacgctt tgatcattac ggacgatgca aatctaacct acgttacagg ggtgcaactt
    26221 caattattag cttcgatacc ctacctgtcg gttatattat tttggagtaa aaacaacagg
    26281 acgctgatct gcccgagcgc tttagcttcc acatatacct ctcagggatg gaaagaaaat
    26341 ttgataatct atgcggaatg cgcagatccc caaggggctg ctatcgctca tctgttacaa
    26401 atgattcaaa ccgaattcaa aggaagagga aatctggggt atatcggtga aagtttaagc
    26461 ggaaagactt tgtcgcaatt gactgaagca ctcccgaatt cgaattttat ggatatcagc
    26521 gactcctgga taagacttcg tgaaattaaa acagaagaag agatcgataa cctatctgag
    26581 gcggcgaaat taaccgacca tggcattgcc ggagcgattc atcatctttc gacactgcaa
    26641 tcaaaaacag aaaaattcct gacggaggat atccgcgttc attctcttga gcgaggaatc
    26701 catatcggtg gctatgacgc ggtctcgcaa gcggtccccg gtgaaaaagc tgatcggctt
    26761 tggccgaatg cgccggcatt cggtatcgga accggcttca catataatca agatactttt
    26821 gttcgtttag aaatgcttgg catttataac aactatcggt caaatgattc aagaattctc
    26881 tataccggtg ttttgaatca aatgcaaatg gatttttttt ctaaaatcaa ggacttgaga
    26941 acatatgcat gttccgtcat aaatcccggt atagccgcat caaaagtata tgaaaatata
    27001 ttatcaaaat caaaagaatt agggataaat gtactaaacg aattgggttt tgggcatggg
    27061 atcggtatag aaccggttga acccccttat atatccgctg ccgatgacgg aataatcgcg
    27121 gaaaacatga cattaatttt aaaattggcg gttcgctatt tgcatggtga aatactggtt
    27181 acaaaagata cactgatcgt aaaaccagac ggttgtcaaa tcgtcggaag atatcagaat
    27241 tgggatattc cttatcaaac agcttatacc ttttaatagg gtttcctatt cttcatactt
    27301 atatcggcag gcaggaataa tgaatcgaaa tccggcctgc tttttttatt cgttaaatta
    27361 aaaaagagca tttcgatttt tgaatcgaaa cgctctttaa gcaaacagca actgtattat
    27421 ccgcccaaat aagcttcttt cacccgagga tcgttcaata attcatgagc cggaccttca
    27481 attgtaatat tgcctgtctc caaaacataa gcccgatttg caatgctgag tgccatatac
    27541 gcattttgtt cattcaataa aatcgtcgtt ccggattgat tcaagttttg tattacttcg
    27601 aaaatctcat ccactacttt gggcgccagt cccattgaag gttcatccat aacaattaac
    27661 tcgccaccgg ttacaatcgc ccgagcaacc gctaacattt gctgttctcc tcccgaaagg
    27721 gtaagcgtag attgcatata acgctcttta agcctcggga acagatcaaa tatcttttca
    27781 taagcggcct tggtaatttc cggatcttta tttaaaagag cagccagtct cagattttcc
    27841 atcaccgtca tattggtaaa aatgcgacgt ccttccggaa catgagtaat acccatcgct
    27901 acaattttgt ggggcgccat attcgttatg tctgtatctt taaaatgaat cgatccctgc
    27961 gcaacttttt ccaatccggt tatcgcacgg agtgtcgaag tctttccggc accgttcgct
    28021 ccgatcaagg taacaatctc acctttatca acataaaaag atatcccttt aaccgcttta
    28081 attgcgccgt aatttacatg taaatcatta acctcgagaa tcatttacgt gaactcctaa
    28141 gtaagcttct atgactttag ggtcattttg tacgacttca ggcactcctt cgctgatcgt
    28201 ttctccaaaa tcaagcacct tgatatactc cgcaatcgac atgaccatct tcatatgatg
    28261 ctcgataatt aatattgctt ttcctaattc ttcgcggaca cgtcgaatta aggtaataac
    28321 atcatcgatc tcgtttgggt tcattccggc tgccggttca tctaaaagga taacttttgg
    28381 atcggtcgca agtgcccgca caatttccaa ttttctttgt tctccgtatg gcaagttgct
    28441 cgcaaaataa tttcgcttat cctgaagatc aaacatctca aggagttcca tggatctttg
    28501 ggtaatccgt ttttcttcct cataatagtt tccggttcgg gtcaacatat tgtaatagga
    28561 gtaattcgtt cgaaatgttt gtgccatctt tacgttatcc agaaccgttg cctgcccaaa
    28621 tagtcgaata ttttgaaaag tccttgcaag tccggcttcg gtaaattgat aaggcttgca
    28681 attggtcatc aatttttcgt ttacgtagac tgctccttct gtcggctggt aaagacctgt
    28741 aagcatatta aagacagttg ttttaccggc tccattggga cctataatgg caaacaattc
    28801 tttttcattc atttccaatg aaaaattatt gacagcccga agacctccga attgaatgct
    28861 gatatgatct gctttcagaa cactcattgg ataccctcct tattactcag ctcctgatgc
    28921 ctttttcgct taaataaatc ggaaaatcca acgcttttaa ttccgccggt cttaagtttt
    28981 agaaaagaaa actcattccc gccaaacata ccgaccggtc tgaacaagat aattacaact
    29041 aacagaccgc catagataac gagtcgccat tctttcataa atcgcaatac ttcgggaaga
    29101 gccgttaaag tgaatgcacc caagatggaa ccgctgatgc tgcctacgcc tcccgcgtac
    29161 agataaacca agaaatcagt agattttgta tagccaaata catccggatg caaatatcgt
    29221 aatgtatgag catacatgct tccggcaacg ccggccaaaa aagcggatgt gacaaatgcg
    29281 atgattttat acttcgctgt attaatcccc attgtgtccg ctgcaatttc attgtctcga
    29341 attgccatac aagccctgcc gtaagatgaa tccataaaat tacggcannn nnnnnnnnnn
    29401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29461 nnnnnnnnnn nnnnnnnnnn nnnnnnnaat gttgcgagca agaaaaccaa tgtttgcgat
    29521 gtgggcggta aaccgccggc attaaaaatg actttggaaa ataatgcgga gacgtacgcc
    29581 ccgacagcca taaaccctgc atgaccgata gaattttgtc cggtcagtcc gtttaccaaa
    29641 ttcaaggatt cggtcatgat gatattgatt ccgccgagca taatcacttg aataatataa
    29701 ttgttcagaa ttcccgctct ttgcagcccg gcaatcagaa tataaatgaa tggaatgatg
    29761 atcacttctg aatttctttt taaccatttc attgttttca ccattctata ctttctcaac
    29821 catcggtttt ccaagcaatc cttcaggctt catgagaagg ataataatca aaaacacaaa
    29881 tgagataccg tatgctaatt ccgaatagac agaagaaatc ataatctcga tgatgcccat
    29941 tatgaaaccg cctaacatag cccctcgaat atcgccgatt ccgccgataa cagctgcaat
    30001 aaaggcttta tttcccaagg tagcccccat ggttaccgtt agaacagggt aactgtttgc
    30061 ataaaatgca ccgctgatag cggcaagtcc cgcaccgata aaaaatgtcg tggatatggt
    30121 cctgtcaaca tcgatcccca tcaacgaaga ggcatcctta tcacaagaaa ccgcccgcat
    30181 ttccattccc atggtagttt tcatcacaaa aaaatagaga attgccataa tgatgacaga
    30241 cagaataatg acaacaatcc ttaccagact aaattgaatg ggaccgataa agaaaagcac
    30301 atctttcatc agtttaggaa atgttctggg attgggtccg ataaaaggga tggctcggaa
    30361 aaagttttgc gtaaataacg acacgccgat tgcggctatc atactggaga ttttcggacg
    30421 atttctcaaa ggtttataag cgaccctttc aaccccgact ccgatcattc ccgtaatacc
    30481 ggcgctggct aagatcacca gtaatgcaac agcgggaccg atcatctgat cacgaaatat
    30541 gagacttacg atgaagaaag ctgcatacgc acccatcatc agaaaatcgc cgtgagccat
    30601 attgatcata cggataatgc cgaaaaccat cgtatatccg attgcgagca aagcataaat
    30661 acttccgagc tgtaatccat aaacaagcaa ctgaataaac ttttccatct tcctccttct
    30721 tctaagtcaa aaaaattgtc tggcacaatt gaagttgtgc cagacaatta tgtcagaaga
    30781 ttaattattc ctcaggtaaa acgctggtta cgtaatgcgc tacgccgtct tttacttgca
    30841 gaattacacc cgctttaatc ggattccggt tttcatcaaa tcgtacatga ccggaaggaa
    30901 gatccaaatc cgtttccatc atcgcatcac gaattccggg tccgtcaatg gtttttgcat
    30961 gctgaatggc attcagcaca atgtgagcgg cttcataagc catcgcagag caaaagctcg
    31021 gtcgataccc cgccaattct gtaaatgctt ttacaaattc ggcggctgtc tctgcttcgg
    31081 gggagaaccc ggttacgtaa taggcgcctt cgataacatc cttgccgacc aaatccggca
    31141 atgccgcata atcccatgaa tcacaaccga gcagaatcgc atcgattccc atgccgcgaa
    31201 tttgctggag gatcaacgga atattgctga tatgtgcagg aacgaaaact gcttccggct
    31261 tggcgttttt gatattgatc aactgcgcgc tgaaatcctt cacttccgca ccgctgaaag
    31321 cttcatttgc gacaactgtc ccgcctaatt tttcaaaagc atcgacaaat gctttatcca
    31381 acccggcgct ataggtatcc gcattgtcgt aaatcgccgc agcttttgtg actttcaaat
    31441 tatcataagc aaaagtcgcg accactttcc cttggaacgg atcgataaaa caggctcgga
    31501 aaatataatc tcccactttg gttaccgctt cgttcgttcc cgtattggta atttgcggaa
    31561 ttttggcagc ttggctgatt tcggccgatg caatggtatc tcctgagtta ttcggtccta
    31621 taatcgccaa aacatgatcc tgctcaatga gtttctgggc agcattcacg gcaatttccg
    31681 gcttggcttc gcagtcttca ataacaaatt cgattttgac tttctttccg tcaacatcca
    31741 gaccacccga agcttcaagc tccttttgaa ccaacttgat cgcatccgtc atgttttgac
    31801 cgtccagcgc ttgttcaccg gaaatcgtgc tgatcatccc gattttgatc acatccggga
    31861 tttctttttc agccgaaaca gtcgcgatcc caacaccgag gatcataaca aaaaccaaca
    31921 aaacatacaa aatccttttc attttcattc tcctttttta tttttttaaa tttattacaa
    31981 taacataata aatttttaca gaaccaaaat acccaagtta ttcttggtta aaccgaaaga
    32041 cggggcagac aaacgtctgc ccctataaaa ctaactattg cttgagacga atccgaatga
    32101 tttgaacggt agggaggctt gtggattgca gaaaattaaa taagagtttt tctgaagcag
    32161 atcccgcttt ttcttcagag cacggattat tcgataggac aactttttgc aattcaacca
    32221 taattttctt tcagagatcc taaatgttta agcttttttg agacagtttt tattctacaa
    32281 gaaaatccta ttttgtcaag aagcaatttt tgttgaaaag agaaaatatt ttgccttttg
    32341 tcaataaaag aatattcaag aatgattatc tgaataaata acaattaaat aatgtttttt
    32401 tatttttctg ataacttatt ttaagaaaaa caagaccaaa attctttctt tatatattga
    32461 aaatttgttt atattgaagc agcaaaggaa ggtttacacc cgatccgcca catgcaacaa
    32521 ctacaatatg atgataagga cggattattt ccggctcatc gtatgcgact gacatggatg
    32581 ccccacatgc caattcgaca attacgcgaa aatcctcaat aaattcaaaa acagcacgag
    32641 ccgcatttgc atctccggtc aaataagaaa taatattgtg attttttgca tattcgatcg
    32701 attccggaga aaccgtcttc ccggccagcg tattagccaa agtatgaatc tcatccaaat
    32761 agatcggttt tccggctttg agcgataaat taaatttagg tgcgccttcg acttcacacg
    32821 caatcaccgg aacatcgctc caaccgtttc ggtctattcc ctgaataaca ccattcaaaa
    32881 gaccgccccc gcctaccgag agaacaatcg catccggttt gacggtttgt tccttcaatt
    32941 catcgacaat cgtggagtgc ccttcccaaa gaagtggatt ttcaaaggga tggacaaaca
    33001 tcgcattttt gtcttcctgt gtaatttgca aggcaaattc attcgcatca tcaaaaatat
    33061 cgccatgaac aatgacatca gcgccttctc tgcgaataac ttccgccata tagttactgg
    33121 cagatttagg aattacgatc gtacatttca ttcccaaagt tctggcgcaa taagcgaccg
    33181 cataccccgc gtttcctccc gaagcacaga taagatggct tattcccttt ttcttggcat
    33241 ccaagcaaac atatgagatt ccacgaattt taaaagaacc gctcggttga taacattcca
    33301 ttttaatttg aaaattctta tgaaatttct gattgatacg agtcaaagga aacaatggag
    33361 tttttagata taatttcata tcttatgcct gatttcaatt tttgtatcgt aatttacatt
    33421 gagtatatcg tcgtttttat cttacaaatt cacttttcat aagacattcc gtcttatttt
    33481 gtcgtttccg aattataaaa aatatccttt atttatatca aaacaaacgg atcgatatgg
    33541 cttggactgt caatttttct tagaataacc tcatcggggt accccagtaa tcggccaaac
    33601 gcaaaggata tctctcgccg tttttgtccg aaatattgat ttttttctgt gagtgattta
    33661 attttttctt ttaaatccaa gtactcagcg attgcatcat ccgtcgcata atacaaaata
    33721 gaattctttc cttctgtaaa ttctgcgctt tgaatgtcgg taacgattaa tgattcctca
    33781 aaacggtact ttgtaccaaa cccatcactg ataatttcgg aagcctctaa cataatattg
    33841 agatttgatg gatcgatagg agggctgatc gcaagccttt tcaccccgca agctaccatc
    33901 tcaataaaag agtttatcat tccgatttca taggaaattt gatctaattg ataagataat
    33961 cgcatttttt ctccaaaata acaatcttta taaatgttta atcacccgcc cgaaatttct
    34021 cttttgtatc acataaaata gctatatttc catttttatc atatttttct gaattcggga
    34081 aatatttttt tattttcctc ttatcaaatg aagggttata gtataattcg ccttgttttc
    34141 gatcatcttc ttataaagga aagaatatgt ttaatcaatc tgatcttccg gaatggcagc
    34201 gagatgctac ttctcctgac caatatcatt gttgtgagac tcgtcgcggg cgagaattta
    34261 ttgttcgtat gatgaccggt tccgatcccg ttcttgcgat tcaaaaattt gcaaaagaac
    34321 aaaatatccg tttcggtaaa gtccatgcca catttatggg cggatttcaa ccctgtcatt
    34381 attatgtttg ggttccggat attgctcatc cggataattg gcatcttgag ggtgaagcaa
    34441 tcaatacgaa tttgactatg ttatgcgcaa taggaggaat gatcggacaa cgccctaccg
    34501 atgatggcgg agaagaacct tttgtcgcaa tgcattttgt cgcaggcgga gcatgggata
    34561 ccggaacaat tgccggccat ttgctcgaag gaaccagagt taaaggttgt atgcaggttt
    34621 ttgttaccga attattggat attgaggttc tgaaaccggt ggatgttttc ggagaagctt
    34681 atactcatcc ggaaaacttt tataaaaacg taacaaaata accattaagt tttcgtttat
    34741 aaagaaggag gtctatcagc agatggtctc cttctttgtc atattaactt cagaaagata
    34801 aatagaattg tacaaaatat cagcgttccc gccatccaaa gccgtccgag acgaaattta
    34861 acttgaaatt tcttctcaaa tatttgcgga aagaacagaa tgatgaccag cataatggtt
    34921 aatcgcaaac cgtcatccaa gtatttttgc ggaattccaa ataagataaa tcctaaaatg
    34981 atccccaggg aaataccgat gatcacgttg tatcgtttcc atttttgatc atcatccagg
    35041 ttcgattctt taacttccgg ttccgcctct tttttttgtt tagcagggaa atcgcgatca
    35101 aattcgttaa ctgctttctc gtctacctga aaagccggat caaataatac tttcccggtg
    35161 acgccctcca atcctttcgg tcttaattct ttcaccaata aattatcggg gttctccgct
    35221 tcggaggcgc gaatatgata attgcttgct ttttcaaaac caaattgcgc gtaaaaagcc
    35281 ggatcaccca acacaacaac cgcttgaaat cctgttcttt cagcctcttc caatgattta
    35341 gagattaact ttttcccgat ccccaagcct tgatatgccg gtaagactcc gatcggaccc
    35401 agacaaatta ccggatgatt taaaccgtta taatcaacaa cttgcgcctt actggagata
    35461 attccgccaa caatccgctg atccgtttcc gcgatccaat ccagttcggg tatataagcg
    35521 gaagattggc gaagttgatg ataaatgtaa tgttcattac atccgggacg ataaacatcc
    35581 caaaatgcct ctcggagtac attttccact tctcggcatt catcgggata tccgtgtctg
    35641 atgattgttt ccatatttcg atttcctatc ttcgggatga aagatgatgt cttttttcaa
    35701 atggatgatc ttattcatcg atgtcgattg caaccaaggc ggcaccgatt gcaccgcaga
    35761 tttccggctc gtcgggaacg atcacttttc cgcccagctt ttcttcgacc gcattcacga
    35821 caccggcatt tcgcgccact cctcccgtca tcatccattc ccgttccagc acttttcgcc
    35881 ccgccaatga taacacttta gcggcaattg accggtttaa tccgtgaata atatcttcaa
    35941 cccgtttccc cgaagcaatc agagaaacaa cttctgattg tgcaaaaacg gaacacatac
    36001 tggaaatcac gatatcttct tttgtgtcga tcccgcgtgt ccccatctct tccattgtta
    36061 atcccaaaga ttgagacatc agttccaaaa agcgacccgt acctgcagcg catttatcat
    36121 tcatcacaaa atcataaaca ttgccgtttt catccaaacg aataacttta ctgtcctgtc
    36181 cgccgatatc aatgacagtc cgtacggcag gattcagaaa aaaagctcct ttggcatgac
    36241 aggtaatttc cgttacttct ttttcccgaa aagaaatccc cgctctcccg taaccggttg
    36301 ttaccgttcg cgaaatttgt ttgcgcttta tatcggcctt caacaaagct tcctccaatg
    36361 ctcgattggc gctttccata atttgtacac cggtcgggat agtagaagac gcaagaattt
    36421 gcttcttgtt atccatgatg acagcgttcg tggtcgttga tccgctgtcg attccggcat
    36481 agtaaccgct cattctttga tttttaccgg tatggttcat tttcctcgta ggtttcacat
    36541 tttcaaaaaa agcttgcaga cgcgtttgca gctggccgat tccctgaatc gtataatccg
    36601 tttcaatttt caacagtgga acacgaagat ttccacgcag aagcgcatat tcaaatccat
    36661 aaaaatcaca aaatgccaca gtattgtata tcactccccg taaatcggga tcttccgtca
    36721 aagatcgacg agatgcaata tctgtcatac gcatacaagc cggttgagac agaagttcat
    36781 gtgcatacca ttccatcaac cgatcaagat tgtttccgat cggagtcccg aggcggcgat
    36841 tgccggtaca cgtttgattt cgaatcggat aaggagaaac ggactgaatt tcattgagta
    36901 tttcatttcc gattctggcg cccaaaacgg cgatataagg gccagaaggc tgctcttcac
    36961 gtgaaaatgc cgatcgaaac gtttccggat gaaattgcgt ttcagtcagt tcttccagtt
    37021 gctgaacaat ccgtaacaat tcctctttat aaagtttttg tcccctttcg tcttgccgat
    37081 gaggtaaagc aatcctcatg attttctgcc cggcggattc caatacatcc gccgttcgcc
    37141 gaatcgaatc acagcaatcc gtgaggacaa gcacgccggt attatttaca atccgatcag
    37201 ccaacaatac ccgcgaaaac ccacacatat tccgatgggt taactcatcc gcagcatccg
    37261 aatccggaag catcgcatta aagatctccg gcttcagccc aaaggatccg aacagttcca
    37321 acggtgtata tttacagata tagcggatca tcgattcgtt tccaacattt caagaaaagc
    37381 ctgcagctta gtaatcatct gaccgtcatt gatgttatta tgatcgcaac cgtctccatc
    37441 taaaatcaac accggatagc cggctttttg taaacattcg cttgttaatc gcgcatttcc
    37501 caaagtctgt ttgcatcccc agtgacagaa ataaaccact ccgtccgctt tcagcttttt
    37561 cgctaagttc agaatatgtt cgctccgtct ctgcccgcga ccgttcatgg gatttcgaat
    37621 caacttcctt gccagtgcat catacggagc attctcatct aattcttcta aaaaatcata
    37681 attcatatca gaaagcaaaa tttgatattt tctgctttga ttaaaaagat ttcggatcga
    37741 ttcttgccaa taagggatgg tatgaaccca tagaattcgt ttttcgcctt cggaaagttt
    37801 tgcattccgg gtatcttcaa gtaggagccg aaaatacttt tctgcatttt ctgtcccgat
    37861 atttacatga gtagggaaaa ttttattcat ttccgaggtt aaatcggtag gtataaaacg
    37921 atctgccaat aaatccagct gttcgcaagc catcctgacg caatgattct ccctgcgaat
    37981 tatttctttt aaacgatctt cgtcgagcct tttccccatt atatgcccga cggcattggc
    38041 cagatcccga agctgatcag agacataagc aacggtatcg gctgattcaa actcgggaac
    38101 atccaccgcg aaacagggaa tatgaaaata atcggcgatg gaacgaaaag tgttcaaatt
    38161 ggcatcgcaa gcaagtgtcg tatatgccgc aaatcgaggt acggggatta cattcgccaa
    38221 cactgctccc agtaatgtct tatgataaga gcaatacgtc tgcggaatcc ccgaatcttc
    38281 cgcataacgg atgaagtagt gctcgcaagc ggctccgttt aaataggctg ccattccttc
    38341 cgtaaaaaaa ggatcaatgt ccattgccca taacagttca cacggaaaaa acaggttggc
    38401 aatcgctgcc tgatccggat tcctgaaagg ttttaatatc gcaccggtat tcataacata
    38461 cgcaaattgt tggtgaggaa gcaaggaccg atcgggaaat atttttgatt gatacaaact
    38521 tacgctaaag ccgatcttca aaagttgccg cgctttttcc ggattcttcg atagatttga
    38581 cccgacccat cccccaaatt tcgagacaat tttattcatg cttgcctttt tcctcgaatt
    38641 cctcaagatt ccatgccgcc ggcacgggtt acttaatcaa accgaattga aatccaaatc
    38701 gatcaggtat ttccgatttc tttctcgctt tgcacaattt tactgaatta aatcgcacat
    38761 tttaccaaat cgaaggaatg ccttaatttt attttccggt ctttattgtg caaaaattct
    38821 catttctgat ttctatcact aatcttttag gattattatc ctcaataaat taagattcct
    38881 cgaaaagcat taccccttat tcgtaattat atttataatc aattatagaa gatggatggg
    38941 gggtcagtgg aaaacattga gcgatataag caacaggcag ctacagctga ttcaactgta
    39001 gccaccgtca aaagtgggaa taggctgaat tttgagcaaa gaaaaaatac agtccaatcc
    39061 gctgaaaaat ccatattatt tctaaaatcc ggatttcttt ttatcaaaac gaatcttgtc
    39121 tttctttttc ataattgtta tggttggttt tcatcattca cgggaaaaga gctctcttcc
    39181 ggtattttta tttcgagttt aattacaaaa atcaagaatc gcagaagatt ccttgataaa
    39241 aatacattga ataacgtttt tattccgggg gatactgatt cttatttaca ggcaccgtat
    39301 caccctattc attttgcttt cgaaatcaga tcttttgcag cggtaaatca tttttgggag
    39361 aaaaaagaaa actttgggga gatgatcaat agatgatcca tatcggagac aaacgaattc
    39421 ttctggttgc gggacattat gggagcggga aaacagaatt ttgtatcaat ttagcgatcg
    39481 atctggcgga acaagggatc aaaacagctt tagttgattt ggatattgta aatccatatt
    39541 ttcgaacgag agaaaaaacg cagttccttg aaagcaaagg aattaaagtt tatggtaatt
    39601 tctataaaac cgaaattacc atggaaatac ccgcactgag cgccgaagta cgaacgccgt
    39661 tggaagatcc gactgtttat accattatcg atttgggagg aaattcagcc ggggcattag
    39721 ttgccattca gttcgaaaaa tattttgttg aaggaactta tgaatttttg gatgtgatca
    39781 ataagaaccg tcccgaaaca tccaccatcg aaggagctgt aaatcatttt catgatatcg
    39841 aagaacgaac gggcttaaaa tttaccggat tgattaataa tacacacctg cttcgtgaaa
    39901 ccaccattga agacatacaa aaaggtctgg aattttgcga ggaactttca aatatactga
    39961 aagttcctct tttatttacg ggttgcccga attatttata tgagcagctg ttatcgtcta
    40021 aactgatgga aaaaatcaac caccaaatcc taccgattca ttttattatg cgagatacat
    40081 ggttggataa aaaactttag aaggagagtg aaatgatatg ggcaagtatc ttgtgaagtt
    40141 caacagggac aaatgtaaag gatgcgaatt atgcgtctct ttctgcccca aaaagattct
    40201 cgaaattgac aaggtggata tgaatgcaaa gggttatcat cccgctaacg taaaaccggg
    40261 gcagatggat gaatgcatcg gttgcctgag ttgcgctttg atgtgcccgg acaatgtaat
    40321 cagcgtctat gaattgtcag gagatgaaaa atgacagaaa aagtattaat gaagggaaac
    40381 gaagcttttt gtgaagctgc gattcgaggc ggctgccggt tttatgtcgg ctacccgatt
    40441 accccacaaa gtgaaatacc tgaatttatg tctcatgaaa tgccgaaacg cggaggaacg
    40501 tttattcaag ccgaaagcga attaggctct attaatatcg cttacgggat cggttgtgca
    40561 ggcggaaatg tatttgtttc atcggcatct ccgggcattg cattaatgca ggaagggctc
    40621 agttttcttt gttcggctga agtgccggtg gttcttttaa atgtctcccg cagcggtccc
    40681 ggaatcggcg gaattcaacc cgcacaggct gactataacc aggttacccg cggcggagga
    40741 aacggagatt ataaaatccc cgttttggcg ccttccacaa tgcaagaagc ggtaaatatt
    40801 atttacgaat cttttgagac ggcagataaa ctgcgaaacc cggtcatcat cttagctgat
    40861 ggaatgatgg gacaaatgat ggaaccggtc actttgccgg aacctaaaca gctttacaaa
    40921 gatgaggcag atatcccgaa ttacaaaccg tgggctctga ccggaagcaa aggaaaaagt
    40981 catcgaaatg taattaaatc ttaccgggct ctgccggaag atcttgaagt acacgttaat
    41041 catttgtttg agaagtatcg aaaagcggaa aaagacttgg ttcgatatga gaccatcaat
    41101 acaaaagatg ccgaaatcgt ctttgtcgcc tttggaacga tggcaaggat cgtttccgag
    41161 gttatcgaag ttctcaatgc cgaggggatt aaagccggca tgattcggcc gattagtctc
    41221 tggccgtttc cgaaagctcc ttttgatgaa ttatctcccg acaaaacgaa ggtagtaatc
    41281 tcgacggaat tgaatatggg gcagatgatt tttgatgttc aactcagttt gaacggaaga
    41341 attccggtca gattaatcaa tcgagtgggc ggaattgtgc ctacgacaga tgaaattgtg
    41401 gaaagagcaa aaaagattct gggagaaatc aaatgaaacc agtatacgaa ccgactattg
    41461 gaatgttgcc ggtagatacc agttattgtc ccggctgcgg gcatggtatt gcccacaaat
    41521 taattatgga atgtataacc gaaaaggggc gcctcggcga tacgatcggc gtctcaccga
    41581 tcggttgcag tgttgtcgca catgaattca tgaatatcga aatgtgtgaa tcggcacacg
    41641 gccgtgcttc ggcggtggca acgggcatca aacgagtcca tccgaatcac cttgtattca
    41701 gttatcaggg tgacggggat atggcttcca tcggagtcac cgaaattgtc catgcagcga
    41761 ttcgcggaga aaaaatcaca actttcttta tcaataacgc gatctttggc atgaccggcg
    41821 gacaaatggc tcccacaacc ttaatcggcc aaaaagccac aacctgtatc gacggacgga
    41881 aaaaagaggt caacggaatg ccgattcgaa tgtccgaaat gctcgcaacc atcgaaggag
    41941 cagtttatat tgaaagagtg gctttaaata ctcccggtaa cgttagaaaa gcaaaaaagg
    42001 ctgtcatgaa agcgctcgaa gttcaggaaa aaggattagg attctcctta gtagaattta
    42061 tttcatcctg tccgacgaac tggggactta gcccgattga tgcccttaaa tgggttggag
    42121 ataatatgat cccatattat ccgttgggag tttttagatc accggaggga atgtaattat
    42181 ggcaaaggaa aaattattct ttgcgggttc gggtggacag ggaattctgc tgatggggaa
    42241 gatgctttcc tatgcggcaa tgcatgaggg gaaagaagtt acctttttac catcttacgg
    42301 agctgaaatg cgcggaggaa ccgctaactg tacagtaatt atttcggata aaccgatcag
    42361 cagtccgctg attcaaacgg cagatgcatt gattgcgatg aaccttccat ccatgaccaa
    42421 atttgaaaat atggttgaac ccggaaaatt tttattcgtc aattcctcaa ttattaaaga
    42481 aaaaccgacc aggaacgata ttcaggtcgt ttctgtcgat gcaaacggac tggcggaaac
    42541 tctgggagtg ccgaaatcag caaatatggt tatgctgggt gcggtcgttc ggacgacaaa
    42601 ggtggtgaaa atcgaaagta ttatcgaagc gatggaaaag gaattaaccg ggaagaaagc
    42661 caaattctta gaagggaata ttttagcgct gaactcctgg aatccaaatt aatatattag
    42721 gagtgaaaat caaatgaaac acagcttcag tattacgcgg tcaacgaacc caaaacaaaa
    42781 accgaatccg gataaattgg tttttggaga aacttttacc gatcacatgt ttatcatgga
    42841 ttattcgaaa gataaagggt ggcacgacgg aagaattgtt ccgtacggtc caatttcgct
    42901 tgaaccggct gcggctgtgt ttcagtatgc gattgaaatg ttcgaaggca tgaaagccta
    42961 tcgtaccgac accggaaaag ttcagctttt tcgaccggat atgaatatca aacgcaccaa
    43021 taataccaat gatcgtattt gtgtgccacg gattgatccc gatttgatgt tggatgcgat
    43081 taaagcgatc gtggatttcg atcgggattg gattcccgac cgaccgggga cttctcttta
    43141 tattcgaccg tttatcatcg cggatgaacc ttatctcggt gtccatccgg cgaaacattt
    43201 cctgtttatc attattcttt caccggtcgg atcctattat ggcgtcggcg gcggttcgaa
    43261 tcaacttccg gcaacaaaaa tctatgtgga agacaaatat gcccgaacgg cagtcggcgg
    43321 aaccggtgcg gcaaaaatcg gtgcgaatta tgtgatcagc cttaaagcgc aagaagaagc
    43381 ggcggaaaaa gggtatgatc aggtgctttg gctggacagt gccgaaaaga aatacgttca
    43441 ggaaatcggt acctcaaatg ctttcttcgt tatcaataat gaagtcatca ccgctccgct
    43501 ggatgacggg accattttgc ccggaatcac gcgggattcc tgcttggcgc tgcttcgaaa
    43561 atggggtatc ccttgttcgg aacgtccttt gacgatcgaa gctgtttatc aggctgcgga
    43621 agatgggacg ttgaatgaag ttttcgcatc cggaaccgcg gcagttattt ctccagtcgg
    43681 agaaatatgc tggaagggaa agaaaatacc cgtaaagacc ggaccgatcg ctcagaggct
    43741 gtatgatgaa ctgacaggaa ttcagacagg taagattccc gataccatgg gttggactgt
    43801 cgaagtcga
//
LOCUS       DAOSMM010000012        41333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1003_length_41333_cov_19.526188, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000012 DAOSMM010000000
VERSION     DAOSMM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 41333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41333
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1003_length_41333_cov_19.526188"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..75)
                     /locus_tag="PK898_03335"
     tRNA            complement(2..75)
                     /locus_tag="PK898_03335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Arg,seq:cct)
     gene            181..1323
                     /locus_tag="PK898_03340"
     CDS             181..1323
                     /locus_tag="PK898_03340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU19100.1"
                     /translation="MEKRILFFGSQITIGGAQRVLLDQALWFRQHGYSVSVAFYYDKD
                     NLLEDWQKCVPFPIRVIGSYQKGKPVPNFFGLIKGFFHLCRFIRKEKPTVIECFTHDA
                     DLIGLTAAWICHVPGRFGSHHGRFHGLSKKKMSLHTKVINSSVTNKIICVSERAKNQA
                     LEEGIRADKIIVIPNGIPLIDPDPDTRKRKRSEIGLTDSNFAIFNVGRLVPEKAQNLL
                     IEAAAQVTALRPEARFFIAGEGPFHDALQTQIMKKSLQDKVKLLGARTDIPELLQAAD
                     LFVLFSETEGMPISLMEAMAAGLPVIASALEGMDELIEDHISGRLIPFGNVPKLAETI
                     LQLIDHPELRMNLGMNAKQTIRNSFTIEKSCQKYEKVFFGGDSSIN"
     gene            complement(1320..2717)
                     /locus_tag="PK898_03345"
     CDS             complement(1320..2717)
                     /locus_tag="PK898_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU19101.1"
                     /translation="MNGIENENKARARYLKRGIYSNPDNSRKIYLNDLAHKLIPSLGY
                     YILLIIAGITCGLAFLYHSKALLVLTLAILPFMGPFFGISLSAVTGSFSFLGKSFLKF
                     LIGIGLFFLSSLGVGGFGRQPVGNISGSTPVASFADDFVFFTDPAGLMILTVIICSFA
                     AAVVIVKGSEHPQALSSGIMAGVLLPIGLAGFCIGLQYFDPLWDCIRTSVIYGLIGLA
                     ASLLAFLFKQVFVPQFKTFLLSGILLVGATLYLCDFTNMINLNLDARFSPMRERFNAL
                     VNPPTVTPTPIPSATATATITQTATFTPTGTITKQPTATLSVTFTKSITPTRPTVTPS
                     ATITFTAVPPTPTKTYTITYTPTVTPTKVMTPTRTIRPSKTPTITFTPSATIVYGIVS
                     VSNDVGLLIRANPGLDSEYVKSQFNGAMLEILGEQKERDGILWVKVRTNDGLEGWASA
                     AALRTATPLATITKP"
     gene            2872..3972
                     /locus_tag="PK898_03350"
     CDS             2872..3972
                     /locus_tag="PK898_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19102.1"
                     /translation="MITYRKPNFRKNFVFFLSGLILLGISLFIQGCNTPVNPTKEQLG
                     KMTESANQQTSVASPTEVLPSATPIPDQKKIIIVRTPDANPGILEIFESALRQLPAEI
                     YTIEETDNLTPATVPEDADLIIFPNLPPDLTQYAQQFPQKQFVVLSLQPQTDSNVWTI
                     QYDIGFQPFLAGYFVGVTANDWRGAGLLPSDSPLYGAAIAETFTNGMKLFCGRCQSYG
                     PPYVEFPLTVLLPEDSAPEAWIAALDQIQDSFPNTFYVSEAALSLPLLQKLSEKQAAV
                     ITAEKPPEGWNGNWLGSVQINLAGALNDIVRRSANGEQAGAVIPELQIETGIQQSLFN
                     QGKKLYLNKLYADLISGLVSPYDPVPPASFEK"
     gene            complement(3998..4636)
                     /locus_tag="PK898_03355"
     CDS             complement(3998..4636)
                     /locus_tag="PK898_03355"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU19103.1"
                     /translation="MTFKFVVYCNMKIGRYPISKRIFDLIVTLIGMVIALPVMAVVAL
                     LVRMKIGSPVLFKQQRPGLNEKIFTLYKFRTMIDRYDADGKPAADADRLTPFGEKLRS
                     TSLDELPELFCVIKGDMSLVGPRPLLIQYLPLYSDEQRRRHLLPPGITGWAQINGRNA
                     ITWEDKFRLDVWYVDHWSLWLDIKILLRTVGKVIKREGISGQNHVTMEEFKG"
     gene            4691..5695
                     /gene="trpS"
                     /locus_tag="PK898_03360"
     CDS             4691..5695
                     /gene="trpS"
                     /locus_tag="PK898_03360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU19104.1"
                     /translation="MENGKIRVFSGARPTGRQHLGNYLGAIQNYVELQKSYECIYSIV
                     DIHALTTVETTKDLKQNCLEMALDWLAAGVDPEKSIMFIQSHVPQVMELHTMLSMVVP
                     MGKLTDLPTFKEKVRMHPQNVNYGLLGYPVLMSADILLYKAELVPVGIDQAPHLEFAR
                     EVVRSFNFRYNTQVLVEPQIKVTTVPKVMGLDGVNKMSKSLNNEIEIAADDETTRKLV
                     MTMVTDPQRIRRTDPGDPDVCNVFSFHKMFSSDEEVEEINRECRIAGIGCVDCKKQLA
                     KNINASLEPLRSRRRKFAEKPDQVWEILYDGADRARKIADQTMLEVREAIGIPIYREF
                     "
     gene            complement(5753..6754)
                     /locus_tag="PK898_03365"
     CDS             complement(5753..6754)
                     /locus_tag="PK898_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19105.1"
                     /translation="MKKTIFSIGFILFIVIFCAGFQAPEGSTEIESGTNEIPPVTKIS
                     WSADSENIFLIGSRSIKRYPVPELDPQSQFYAASADQFIQDYCPGENVIVTLKEDRQL
                     LFYEAGSVKYQSGIKLDYPAMGADCSNDKEILVPSLDEIAVELYDISSGNLMKKLTGF
                     QTAAPVYGATFSPDGKFILWHARASFRLQNIADESWGSPIEFQDFIDVYRLSPDSKEL
                     VVAAPSEDYSKNNLIFMDTQSGEELSRIEIGEQPIGAIAYHPNGKFLMAADSTSLYRI
                     DLTDKKVTWKEQMVKSDQNVSDPIRIADISFSPDGKYLAVLQSDEQLSLFRIEEILQ"
     assembly_gap    6809..6908
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6909..7225)
                     /locus_tag="PK898_03370"
     CDS             complement(<6909..7225)
                     /locus_tag="PK898_03370"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOU19106.1"
                     /translation="MQFVMIFSLIIAVLSVMFALQNTAVVSLKFFIWTFESPLGLLLL
                     IALTVGAIIISILTLPSWFKSKRIKSIHQKELGELEENLAKYRTDLIDTQNKNKDLRQ
                     KVL"
     gene            7344..8774
                     /gene="radA"
                     /locus_tag="PK898_03375"
     CDS             7344..8774
                     /gene="radA"
                     /locus_tag="PK898_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU19107.1"
                     /translation="MSKTHTHYVCQQCGRTNPKFLGRCPGCGSYGTMIEEIIPEENPQ
                     SVSGILHNNPIPKALRDIDEKAEPRVDTGIGELSQVLGGGIVPGSVILIGGDPGIGKS
                     TLMLQTALHLSQKGLVLYVSGEESERQIKMRATRLLENNSVIREIPEKLYLVTETNLD
                     VIFSHVQSLKPDYLIIDSIQTVQLPILESTAGSLTQVRECAGKLRELAKTSGITVFLI
                     GHVTKEGMIAGPRVLEHIVDTVLYLEGDRFLSLRLLRSVKNRFGATSEVGVFEMVESG
                     LIEVKNPSELFLAERMVNAPGSAVVATMEGTRPILVEIQALTNPTSFGNPRRTPIGVD
                     MNRLFLISAVISRRIGLRLSEQDIFVNVVGGMHISEPAADLAIAAAIVSSVKNQPIRA
                     DAVMIGEVGLSGELRGSGKLSSRLKEARKLGFTTAVIPKSIHHKEKYPEGMNVKEFRT
                     LREALMFILLPVAEKEGTSSDSDIIN"
     gene            8791..9345
                     /locus_tag="PK898_03380"
     CDS             8791..9345
                     /locus_tag="PK898_03380"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HOU19108.1"
                     /translation="MEKTIETKVRDLCIQNPVNHVRKNHALEHATVHILEKKFPDKEF
                     AGYSVERGFWIIGKLSIQEIQEAADAAFARLSHGEKDLAVHPGCGTNLATASLLTSVS
                     AVLTFIGTKSEKDRLKRFSSLLLISSMAIQVSKPLGFAIQRNLTTDPDMNGMEISGID
                     TGQIREYPYFFIRTVYQKPCESSL"
     assembly_gap    9418..9517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9518..10015
                     /gene="holA"
                     /locus_tag="PK898_03385"
     CDS             <9518..10015
                     /gene="holA"
                     /locus_tag="PK898_03385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU19109.1"
                     /translation="AVGNDTRAAKQELEKLLLYVNYGRPIDSMDIQELVTGSASVSIF
                     DMVDALASGNAKEAFRTLDLLMQDQEIPVLFAMIIRQFRLLIQTREIMDEKGNIATVQ
                     DELNQHPFVAEKLWRQASRFRFLQLKSIYRKLVEMDFAFKTSQPDSRAALDVLIADIS
                     LMVQD"
     gene            complement(9999..11114)
                     /locus_tag="PK898_03390"
     CDS             complement(9999..11114)
                     /locus_tag="PK898_03390"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HOU19110.1"
                     /translation="MDHKILTILLIVFTVITGCSSSVWADQEQKVINPLTGIETDPNW
                     LSYPPFIIPYTKFPDKFRSSAGLSFAPWVFEMYSGDGESRPMALFYGSIPESTTGVTP
                     YIGSVSSAVLGLESLRKQYMAVLITAGNPDLVKKAGISNLETWYGESGSEKYPDLPVQ
                     RFIEIMEKWKKRLGSPDIEGISLPFSAAIPEGSRSGKTLFIRYAVFNQILWEFDEADG
                     LYHRSQNSVSDPNPVKDIDVLNNQQITAQNVILLFADHSLIEDGVNFTVNFNYVKKNP
                     ALIFRDGLCYQAFWTTKAEEYEIQNQKLRPIRFLEADGDAFHLKPGKSWVHVVQINNP
                     VYEIAEPTGNQTEDGSGHWKVPYISVKPNEKNLNPVP"
     gene            complement(11221..11292)
                     /locus_tag="PK898_03395"
     tRNA            complement(11221..11292)
                     /locus_tag="PK898_03395"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11258..11260),aa:Asn,seq:gtt)
     assembly_gap    11341..11440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11589..11714
                     /locus_tag="PK898_03400"
     CDS             11589..11714
                     /locus_tag="PK898_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19111.1"
                     /translation="MKIKMDIKEARRITVIEQAIEKKISDREAAQLLEWSAAYGK"
     gene            11843..12787
                     /gene="ppk2"
                     /locus_tag="PK898_03405"
     CDS             11843..12787
                     /gene="ppk2"
                     /locus_tag="PK898_03405"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOU19112.1"
                     /translation="MAKEKKKKKKKIDQKIGSGQTELSNEKKENEKKKDVISAIPTSK
                     STSIPCGMPYTKPPLSKKEYEKELEKLQIELVKLQGWIKEKGLKVVVIFEGRDAAGKG
                     GVIKRITELLNPRIVRVVALPVPTEKEKTQWYFQRYVAQLPAAGEMVLFDRSWYNRAG
                     VERVMGFCTEEEVTEFFRSAPEFERMLIRSGIILIKYWFSVSDEEQEKRFQDRINDIT
                     KRWKLSPMDLAARSKWVEYSKAKDEMFYYTDTKQSPWYVVNADDKQKARLNCIHHLLS
                     LIPYEDLTPEPVVLPPRQSDEGYVRPPISDQTFVPEIY"
     gene            13003..14316
                     /locus_tag="PK898_03410"
     CDS             13003..14316
                     /locus_tag="PK898_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19113.1"
                     /translation="MNARKIPFLLFLFVIFLIPFSAEAAGDQTNPNLFPVTVAKNIDP
                     GLIDAINEGDSGGEIGLALIGSDTTVGYYKKCSTDAYFYFIVANNSGATVGTVLPRWA
                     KIDGRAYFTDSSAYLCKELAAGATPSNNFADADSGSACILQNNQLRVGAGKAVSVRGK
                     IVNFSHELTRGNKKDSDPEYLHINFYVQINNSKKQGTGALESKGSLCDGLNFLPVTPH
                     NYVDGGYFIDPSGRRVCSMNYFFIVENPDLFYSTVVELPRWVELDEQRFNVGCKYLYP
                     GCKVSGFRHYAWAILDEDDTYIGPKYYFCDQDRAVGNNCYEENSLWWLRVPPNTKIAV
                     RGMVNNLCNVPAANPLQNSELVVGFDVSDAGSNKWWLDGIVEGTESKDLANNSKDINF
                     IDEPSRLEDRDKDEPVDRYLLEDPSLVDLEEEAAQPIIIEETSEP"
     gene            14505..15272
                     /locus_tag="PK898_03415"
     CDS             14505..15272
                     /locus_tag="PK898_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOU19114.1"
                     /translation="MAIKVLAQDAEQTYRVKKGDTLSEIAARFNLSQEQLIKANPQID
                     PDLLMVGEELQIPPADSISYNRYLQNRYNEYLAIQPVDCYPNPDQTAECLTQIKNIGD
                     RTTYNIRLIAQTTDSEGGIRESVSSPIVNQVLPGESIPVLFRFDNRLSDTDTVQCTID
                     SLELSKDTSLSLRLDESQWSKEIVFSSDKKSAKVILHFEQAAMEKTKSILIAAYNEDG
                     KVVGIRGKTNSVEQDLSLFVYSLSGMIQDISVWVEAY"
     gene            15282..16280
                     /locus_tag="PK898_03420"
     CDS             15282..16280
                     /locus_tag="PK898_03420"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU19115.1"
                     /translation="MTTILVTGATGFIGSALCRELCERGYQVIGFHRFSSSLKMIEGL
                     PIQRVIGDLTDESSIENAFSYHPEIVFHLGAQQFSGRNLTRLMDVNVGGTRRIFQASF
                     NAHVKRIILMSSAVTMGTAELSPDRHRSPIPVGETHGWDRTLESWPFAWSKLLAEYET
                     QWAIAYGMDIVICNPMFVIGPGDYYRRNTGLIARFHDKPPKFFIEGGINLIHVNEVVQ
                     GLIQAMNYGEKGRRYLLSGTDRSFQQFFGDLAKLSGTPAPRMQLPTSFSNRLYAYRNR
                     HPDLFPVEKTDINLLRMAGKYFYYDGKNSRLALHLPPPQPIEPALQETLNWFRENK"
     gene            16277..17317
                     /locus_tag="PK898_03425"
     CDS             16277..17317
                     /locus_tag="PK898_03425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU19116.1"
                     /translation="MNRETVCLLPEIKSVGGPAAFQQRFITAAAKQGITAHFDSSRKD
                     IGAYLVIGAPRKFIPTLYSARQRGIPVIQRLNGMNWIHRVRPSGWLYGKKADLANNRL
                     SFFRKHIATGIVYQSPFCETHWNEVYGVLSDKPSRIIYNGVDLNEFKPIVPEFSSENI
                     EILIVEGNLEHGSEQNLEQALSLSMALSRRTSQKVHLRIAGNVPEAIREKVFERMSDS
                     TAVVSVDFLGIIEKSRLIELENSSHMLFSVELNPACPNSVIEALACGLPVIGFDTGAL
                     KDVTGVGGIIVPYGTDVWKLEKPILFPLIDAAEKVYRENQKFRKAAREQAESHFSIDK
                     ITEEYLSFLFGS"
     gene            complement(17330..17845)
                     /locus_tag="PK898_03430"
     CDS             complement(17330..17845)
                     /locus_tag="PK898_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOU19117.1"
                     /translation="MGPFSPSSFYTEESILAYLKVHGISYTVFHHPAVYTSAEADVYT
                     ANLPGFRAKNLFLSNNDETRHYLYVCHENQRANLKQLAKIMNDSRLRFGNERNLVDFL
                     GITAGAVSLLALVNDPFNRIRVIIEDEVLQAESVQLHPLVNTATVIISREGLKEFLSV
                     TGHSIFVVSSK"
     gene            complement(17852..18361)
                     /locus_tag="PK898_03435"
     CDS             complement(17852..18361)
                     /locus_tag="PK898_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19118.1"
                     /translation="MRKELISWLEVDKLIDHLIPQFHDEFDVILMIQKGGIIPGGILS
                     EALGIQDIYLTDIDSPKEFELEKQRSDPRFISWPTIRLFPDNSLIEQKKVLIVGCAWG
                     TGRCISAVRNRVSGAGGMPFTCVLHYNHRRNLFPEEKPDFYAAITDAWIIYPWESPKG
                     KNLILSTSV"
     gene            18640..19173
                     /locus_tag="PK898_03440"
     CDS             18640..19173
                     /locus_tag="PK898_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19119.1"
                     /translation="MKATRFIWFIIMIAAGIAIGLYYTWIVHPVKLVDATFYDLRQDY
                     KADYVLMTAEIYNDNPNLFQTMIRLDRLLEESPEIAVANAIRNADQLGYSETDIDMLY
                     RLNEAIGGNAIETPDAEDIFIEFDPQTFVNTETAVPIRELSAPAEMPTEDNPFIDQSL
                     SQPTLVPSDEPNPFGEP"
     gene            19173..19676
                     /locus_tag="PK898_03445"
     CDS             19173..19676
                     /locus_tag="PK898_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19120.1"
                     /translation="MVETGRKGPYYLLTGLIIGLILGIFYGWVINPTRYVDISPEALH
                     PDQKKQYLLLVAESYQANEDIGRSYYRIKQMTNPVNIDELRAMLLEMESDPDYAEQFE
                     VVRNFINDLDSYLKQQNPDNKISTQQVQGLLNPSDAREKGTPATAIDEQFNKETANPE
                     SFYVNSN"
     gene            19734..20291
                     /gene="frr"
                     /locus_tag="PK898_03450"
     CDS             19734..20291
                     /gene="frr"
                     /locus_tag="PK898_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOU19121.1"
                     /translation="MINDIITDSENRMKSAINVLQEDLEGIRTGRATPALVEKLTVDY
                     YGMETPFNQVANISIPDAKTILIRPFETNLIRPMEKAIQASELGLTPSDDGKSIRLML
                     PPLTEDRRRDLMKLVNNRLEETRVAIRNIRRDTIKELKDLEKAKEISEDELKKAEEKI
                     QKLTDQWITQVDEIGKRKEAEIMEV"
     gene            20297..21022
                     /locus_tag="PK898_03455"
     CDS             20297..21022
                     /locus_tag="PK898_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOU19122.1"
                     /translation="MAQTKLNIEPSRIPRHVAIIMDGNGRWAKKRGLPRLAGHRQGVE
                     NLRQIIKACVEFGIQYLTVYAFSTENWGRPEDELRGLKQIFNDAFSNELEELIKNGVR
                     ILHIGQRDEISPDLLQKIDRAVETSKTNDKLILTVALNYGSRNEIMHAVRKIVASGIP
                     PEEVTEQVISDSLFTAGTPDPDLVIRTSGEQRLSNFLLWQSAYSEWVFPEAYWPDYNR
                     EELIKSLEEYAKRDRRFGKLSSK"
     gene            21026..21853
                     /locus_tag="PK898_03460"
     CDS             21026..21853
                     /locus_tag="PK898_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HOU19123.1"
                     /translation="MDNLKTRVISAAVLAPLFIFLIYLGGIPFLFASLLIIGIGSWEF
                     WRMYNKEQSNRSSAVLLIGLSILTGASRYFLDFTIASSVLALCIMIAMLWGNISYERK
                     NQKAADGFAVMTTGIVYFGWIGSYLITLRQSENGFWWVIICLLLVWLSDTGAFFVGSK
                     FGKHQMAPLVSPHKTWEGYFGGVLSTLVIGILLFLIIPPVSKILKFPQMILLALLIGL
                     ITPFGDLGESMIKRTFGFKDSSNLIPGHGGIFDRMDTVFWTMVIGYSMYTLINLGLI"
     gene            complement(21834..22946)
                     /locus_tag="PK898_03465"
     CDS             complement(21834..22946)
                     /locus_tag="PK898_03465"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOU19124.1"
                     /translation="MESTNRNHHSLILPLIVILCGLIFQLQASGVLGNSQENLLSKFW
                     PIVIIAAGLDLYFDGKRLFGAVVLSFLGVALILLNLNGNTKAWEIFMTFWPLFLIVYG
                     FDMLFSGKSLSSIIIPFIVIGAILYVAFINKGEFSVSSLQESFNFSKISELVSTRIGS
                     IDSEGIKNIDYPLTSQSLATFQIKVPSGKFQLKSKEMNGHLMTGTIRLAEGESFNDKI
                     DQSDSGSIYMMNGTAASQTGSSEAIWDLQLNRQIPVVLMVNMANGYQMVDLRDLDVAG
                     VNLISQVGNIDVMLPYAASVPVNISTQSGDIRIFVPASVSAYVTVQNATDVFYPETYM
                     QSGSQIFPANGLMNGSQVVVNANAPAGVVKVILNPN"
     gene            complement(23023..24096)
                     /locus_tag="PK898_03470"
     CDS             complement(23023..24096)
                     /locus_tag="PK898_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOU19125.1"
                     /translation="MKKNCRLTRTIRLLILFVFPFIFSSNTLSPFRIETITLTPTVIP
                     TERLFREEIMETINQARREKNLPALRWDESLANAADQQAAYLAKIGKLTEKGLNNESA
                     KSIAADFGYGGGSSFQIEQCIAMSWSDTEPSYIVKNIWLNQAEDRAALLNEDVVHIGV
                     GESLDVGRHRYVVAYLGRLDSGTEAYTPLPTYDQRTPKPQLSYTPTAKMILISTPNPD
                     GSIIHKIQEGETLSEIAYAYHTDWDTLSNLNDLDLKNPVIIEGQDLLIRPKFTQTLTP
                     TVTETPRPPTRTPRPTFTTDSAAIKGQPVVTPQPTETELTEWNASQILIKVKPYRKMI
                     GIMLIVISACGLVFSFILRKHDH"
     gene            complement(24093..25097)
                     /locus_tag="PK898_03475"
     CDS             complement(24093..25097)
                     /locus_tag="PK898_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU19126.1"
                     /translation="MRKSVLSFQFLFILSVLAFATCFIIYPVYAQDANSLIAEVNALR
                     ASYGLSPYTVDNSLSSLAQAQSEYQASINMTTHNRPDGSNIPARSENVCGGLNMTASY
                     CVKQMWTDPDHLYTMIGLDSGTVGAGAASAPDGSIYYTLMVNSSGNDTNLDKSPTLAV
                     TAAVSANQNAGSGFVDIEALPVQPGTFATSTPELDGSIYHTVQQNETLWTIAINYGTT
                     VAQLQALNGMAENDVSVRAGQRILIHYGGTPVSDTLTPTVTKPPATNTPKPTSTMTQT
                     LPPLPSLTPTITATPTPGPLIKHIDFFDKPAARRLGLVLVVFSGIGLLITIFFGFFRR
                     "
     gene            25294..25989
                     /gene="lexA"
                     /locus_tag="PK898_03480"
     CDS             25294..25989
                     /gene="lexA"
                     /locus_tag="PK898_03480"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOU19127.1"
                     /translation="MARMGKMMNDRHRKIMLFLEEYQKNNQFSPSIREIGNAIGVNST
                     SLVDYYLRQLAQMGYLERNDRVSRSIRILKPFTEESQTAKNMISYSRQEVLRYPILGR
                     IVASEPIPVPGSDLAYFDPESYIEIPKKMIPASEKETELFVLEVSGDSMVDAMINDGD
                     KVVFRQANQASNGDMVAVWLSNNDETTLKYFYREGSRVRLQPANPTMGPIYIDDPEQV
                     HIMGKVVMVIRHL"
     gene            26035..27177
                     /locus_tag="PK898_03485"
     CDS             26035..27177
                     /locus_tag="PK898_03485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU19128.1"
                     /translation="MNNQARQKKIAIISSLYMPHLGGVEKYSQSLARTLTADYEVHVF
                     CMNTESVPAESRDGDIFIHALPCGGFFKGRLPVPTPKAIRRTKQIFRETGFDFGIIQT
                     RLYPFNLQAAKILKEFQIPFFLIEHGSAYVTFGNSLINRLWEYYERHQANILKRYCSD
                     FYAVSQASLDWLNHFGIQGKGIIHNSIDPREFEGIPAGWRKSVGIPSEAILLTFAGRI
                     IQKKGIVTLLTAAERIEEERLHIIIAGDGDETLLSPWKQNSRIHFLGKVPHRVLLEIL
                     KDSQLFCLPTTYPEGLPTVILEAGYFGVPVIATDMGGINEVISDNKTGKIIPADDPES
                     LRSAILELIRDEKERNLFGSELQKKVSAEFTWQKTALEVKKIIESV"
     gene            27309..28247
                     /locus_tag="PK898_03490"
     CDS             27309..28247
                     /locus_tag="PK898_03490"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU19129.1"
                     /translation="MKVFVSGVAGFLGTPLANQLVKEGNEVIGLDDFSAGNAEGLDPA
                     VRLVRGNITDRELLWKLLQGVDCVYHLAAKVIVPDSILYPSEYEKVNVGGTVTLLEAM
                     RDVGIRHMIFSSSGTIYGFQKEQPLKEETTKPKPASPYAVSKLASEYYINTIGRLWGL
                     EAVCLRIFNAYGPRQGFSFTHAPVIPTFLKQSAARGTVIVHGDGHNTRDFVYVDDVVT
                     ALILASKIRNPQELVINIGSGIETSVQEVLDLAIKITGSKPEIVYNQRRLGGPDRMCA
                     DLTLARKLLNYEPKVSLEEGMRLTYALDRRLHKQTG"
     gene            28375..28536
                     /locus_tag="PK898_03495"
     CDS             28375..28536
                     /locus_tag="PK898_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19130.1"
                     /translation="MDLTDWLLCLGTAAAFGFAFWILLPPYGWIAGIAVAGLMLYRAK
                     KKRDDLKQK"
     gene            28554..29072
                     /locus_tag="PK898_03500"
     CDS             28554..29072
                     /locus_tag="PK898_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU19131.1"
                     /translation="MENKIPAGNNKVVSLAYTLWVDGEPIDSADVNNPLVYLHGHNNI
                     IPGLENELNGLFPGEKKTVIVSPEDGYGELMPDSIVELDRSLFPEDYIPALGSVLHLQ
                     DESDQVYTAYIQDFNENSVIVDLNHPLAGKELKFDIEIVEVRDASEGELKAGHIHHDC
                     SSCGGCEDGSCE"
     gene            29069..30154
                     /locus_tag="PK898_03505"
     CDS             29069..30154
                     /locus_tag="PK898_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU19132.1"
                     /translation="MKNYKIPMVPGPVRVTPVAYRSLTSDFGAGYCEDEFFELYQTVG
                     KKLGILLGTQSEVIIQSGEAMSILWGAIKSCVLPGEKVLAISTGFFGEGFADMARAIH
                     ADAQLVEFGYDETVHDLDRIESAIKTYQPKVITIVHCETPSGTLNPISEVGQLKKKYN
                     VPIMIVDAVASVACTEIEGDKWNADIVMGGSQKGLSLPPTIGFCSVSEQAWDIIRKVD
                     YTGYEAFWSYHNIPQKHDHPNTPDWPGIAALNDVLDDLMINEGLSNVFARHQVVAEFV
                     RKSLTEIGYRLFPKPEAIQSPSVTAVYVPADRTFEAFDQQVRTYGMGIGGSYGKIAGK
                     VFRLGHMGTQATMTNARSAIDVLQQVI"
     gene            30165..31661
                     /gene="crtI"
                     /locus_tag="PK898_03510"
     CDS             30165..31661
                     /gene="crtI"
                     /locus_tag="PK898_03510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOU19133.1"
                     /translation="MKPLNVIIIGAGLGGLACGIRLAVEGCKVKIFEKNHFAGGSLGS
                     IRLKGFTFNTGAEYISAPFLFDQLYEYAGKRRIDSFKFRDCDPIFQAVFPDFKRFALY
                     SNPAETVKLNPMLSGADLQGFNSFTEESAAIFDDIFFDYCGRPVDENTFRFRTNLWLR
                     RMNPQKTCEEYANEKFQTPELQQLFGFWPLIGGGNPKSATHLFRAFSQMFQRWGTAVP
                     DGGMDNMVQAIVRLFRESGGEIFYGTEVSEIQIFNHTVSGVRLKDGRIQQADLVISDA
                     DAAATYRYLIDSDRTRYDSVNQAKTKISGISAFVYHLGTRCILQERAPLAPLNILFPE
                     DYDRFLNELFDQKTLSDHPLLILRMASKIIPDNAPFGCEALSVIVPVPNLESINPWDH
                     ISYSYRNHVLDLLQNYFLTDIRTNLIVEEYLDPIIIKNRLGSHAGSILSFQPELQKNG
                     EIRMANRCRDIRHLYLAGNGAHPGATIPGVLLGAANTVQVIKEDFDKE"
     gene            complement(31675..32781)
                     /locus_tag="PK898_03515"
     CDS             complement(31675..32781)
                     /locus_tag="PK898_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19134.1"
                     /translation="MPINLNPVPARIDPKVLAALAKPQSGNPFSLSEKIEDRFREMFF
                     PDAASINFFNCSREGLYEVALNSFASGPVLIGMNGPFSQQWQTIAKSLNIDSEIIDTA
                     FGSGFDEDLFEKALDEESYAAVFLVEIDPFTGTQIDIAALSGMIRKKLPDALVIVDCS
                     ASFGSVRALDLKSEADALLIGSEISLGLPPGLGTVILGERANFKSIGFSGTGWSLNYA
                     RQFRDIHSKSFRSLIPFQLLNALDRQLDLIFLEGIENRIKRIVSLSEKMKGWADANRF
                     EIFTRPQFRSPTVTVLKKPLPFTVQNLTDFLSSYGIIIGNCPDDMRDDYFVIAHMNQT
                     TEEEMTLFLDVLDRFLADYDTKKSIPVVSKLSPQ"
     gene            complement(32875..33084)
                     /locus_tag="PK898_03520"
     CDS             complement(32875..33084)
                     /locus_tag="PK898_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19135.1"
                     /translation="MAKKNTTKGKAPIKTTATAVSSSGNLPLAARAPKEEFNPDYTYV
                     IRNLKKIGTLAAVFIAILIILSFII"
     gene            complement(33111..33995)
                     /locus_tag="PK898_03525"
     CDS             complement(33111..33995)
                     /locus_tag="PK898_03525"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOU19136.1"
                     /translation="MKRKKYADELTEEELRFRLMQKKTIGRDARIASYRAGGRVMDAA
                     NAPINSELKWNSDRSEKEVLKTAPPQKKFDKILLAIELLAVAGVIVMLVIGSQILQNI
                     NRESAKSMILPTLTPTPLIQAVVLPGGHTPPDESGVGTFNESEIPEHLRGIVSVMAPQ
                     PTIPAGMEQVIRLRIPAINVDAPVVLGDDWEALKAGIGMNLYSAAPGKNGNIILSGHN
                     DIYGQVFRDLDQLMPGDQIIVLTEKNAYTYSVQNIEVVLPNQVEVMAQTTDPTVTLIS
                     CYPYLVDDKRIVVTGKML"
     gene            complement(34093..35082)
                     /locus_tag="PK898_03530"
     CDS             complement(34093..35082)
                     /locus_tag="PK898_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU19137.1"
                     /translation="MPEQSFPFIFIHLNQSCDFISLFNSLVKLGGRPVAFLSAEPVRT
                     ENEFIRSVPVVPFSGNNFQEALGNLLFDSEKNTGIESFFVICSDDAVISDSELNEQLA
                     SARKNPQAFYLLEILTTQESVEDNGIVRMIRWIRRKLEGPKASLWIIPPQAITSANPY
                     SEDKKFSLFPWLLKCERQGIPIFLTRVNRKNDGAVAVNSLRRINLLFYTLNTFLRYSG
                     SSVLSFLVDNTVFFLGIQIGFGNLLALVTGRVASLLVNYFLLKSVVFKPKNEKNRSFA
                     KYVGLVIFSGSVVWSLITIGEKIIPIHPIFLKLAAELGMFFFNYYVSKTFIFN"
     gene            35220..36503
                     /locus_tag="PK898_03535"
     CDS             35220..36503
                     /locus_tag="PK898_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU19138.1"
                     /translation="MKIAIIGSGLTGMTAAWNLRKQGHEVTIFEKEKEPGGLARGFRE
                     PQWQDSVECFYHHIFTGDSDILSLFKDLGVSNSVEFRSPKTVMYYRGKFYPFDSIPSA
                     ILYPGLGLGSKIRFGFVGLYLRLFSPWKEMEKVTAEEWMLKYAGEHVYRSMWEPMMLG
                     KFGEKYAKEVNMAWLWARIHSRSTSLGTFRGGFQTFINIFSEKLIANGVEIRFESNVT
                     QIKRQKESSITVTADGKNDDFDRVIATISPAAFSAISPDLPADYRQSLLDLKNMGAVV
                     MVLSLKHPLSPEGYYWYNLPKSEGFPFLSLVEHTNFVPRERFGNETIIYAGDYLETDH
                     EYFSLPKNELLQRVLPGLKKINPNFEEDWVINSWKFSAKYAQPIPFINQSEHIPSIET
                     PINGLYLASMSQIYPWDRGMNYAVRMGNTVARMIG"
     gene            36940..38340
                     /gene="dnaA"
                     /locus_tag="PK898_03540"
     CDS             36940..38340
                     /gene="dnaA"
                     /locus_tag="PK898_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOU19139.1"
                     /translation="MAQTDETKITTYKITPEKAWEAAIGQLKITMDKQTFSTLMGKSE
                     FVKYENNTFFISVPNTFTRDWLEQRLTETVIRILSGIMEGQQFVQFVLESNVNAIREE
                     KTVLSQPAVATVSASQSVCREGAINPRYTFDNFIVGPSNNLAYAASRAVAAMPATAYN
                     PLFIYSGVGLGKTHLLHAIGNEIIRNQNKAVLYVSSEEFTNDFINSIQRHENTAFREK
                     YRNIDVLLIDDIQFIIGKESTQEAFFHTFNTLYQQDKQIIITSDRSPKSMSTLDERMR
                     SRFEWGLTVDMQTPDLETRIAILSVRAEKVGKSIPRDILEMIARQFPNNVRELEGALN
                     RVLAVSDLCGKTLDKDLVLMSLGELLPQQNATPLEQILNVVSKVFGVPTEKILSRDRC
                     KNVALSRQVVMFLLRNEENLSLPQIGKELGGRDHSTVIHGCERVASLLSSDVRFRNLF
                     EKVHEQLYGEPCFVMA"
     gene            38579..39265
                     /locus_tag="PK898_03545"
     CDS             38579..39265
                     /locus_tag="PK898_03545"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19140.1"
                     /translation="MSKRNLIIIFAVVVISLAGFGAAYASGSTQYSWPNNCGNWNQCN
                     CGWNNCCCNYYVPPQPSLCASFIADVTVPDGSYIAPGSSFTKTWRIRNNGTTTWNTNY
                     KLFFSSGSQMSGPSWVAMPYNVAPGQTVDLSVNLIAPTTGGTYRGNWKLQSDTGVTFG
                     VGSTCQVPIWVEIKTQQPVYYNNCCYGNCCCVYPCNCPCTPAVPSTPVNWQNWSTTQA
                     PAWPTWPDWN"
     gene            39524..41140
                     /locus_tag="PK898_03550"
     CDS             39524..41140
                     /locus_tag="PK898_03550"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19141.1"
                     /translation="MYKKQFFTIIAMAIFTVLIGVIAVQASPDVQGYYGFNNPLWGNM
                     DQPWNYNYNNSYNYTYNNAYSYSPQVCNQCTYACNRAEFVKDVTVPDGSYIAPGASFT
                     KTWRIRNTGTTTWTTAYRLVFSGGDSLGAPSSVAFPYSVSPGYWMDISVPLRAPNAAG
                     TFQGNWMLQSSDGTLFGVGCNGQVPIWVKISTVSNSCATVAYSCNNCNVNYCNPACYG
                     SYYNQITKNPYCNNKIRSVNDITIPDDTEMDPGTSFRKTWSMKNGGTCVWNEGYSLIF
                     SGGDAMGWKTPVFLPNPVYPGETVEISIDLKAPNIPGDYRGYYKLRDNLGFTFGYGSY
                     ANKAFWVDIKVTGSPLPPTKTPTPTPRPTATPTPTSTPAARTAAGSGQELSLVIQSED
                     DDSLVEPLEDPLLLDDDESEADLSNVLEEGEGVNQKSIAYNACGKQRIEINFNKPDIY
                     NITWYVTNAGSAVWNSDKYRVVNSGLSDNLVMMGTDITVPATNPGEEAMVSMSVKVKD
                     VADQSDRWLKYYLTDGNESFCEVYFSLPIF"
BASE COUNT        12018 a         8715 c         9062 g        11238 t
ORIGIN      
        1 gtgccccggg agggactcga actctcaacc taagcgttag gagtgctttg ctctatccta
       61 ttgagctacc ggggcatctt acgttgcgat tatatcacga agggccggta attcctgcag
      121 gtagaatcag ccatattccc tctattattt caatcgcggg taaaatttga tcagagaaaa
      181 atggaaaaac gtattctttt tttcgggtcc caaattacaa tcggcggtgc acagcgcgta
      241 ttactcgatc aagcgctttg gttccggcag catgggtatt cagtcagtgt tgctttctat
      301 tatgataaag ataacctgct tgaggattgg caaaaatgcg ttccttttcc catccgagtt
      361 atcggcagct atcaaaaagg caagccggtt cctaacttct tcggtttaat aaaaggattt
      421 tttcatttat gtcgattcat tcgaaaagaa aagcctaccg tcattgaatg ttttacacac
      481 gatgccgatc tgatcggatt gaccgccgca tggatttgcc atgttcccgg tcgatttggt
      541 tcacatcatg ggcgtttcca tggattgtcg aagaaaaaaa tgagcctgca caccaaagtg
      601 atcaattcat ccgtcacgaa taaaataatc tgcgtttccg aaagggcaaa aaatcaggcg
      661 cttgaagagg ggatccgggc ggacaaaata attgttattc cgaatggaat tcctctgatc
      721 gatcccgatc cggatacaag aaagagaaaa cgatcggaaa tcggactgac ggattcaaat
      781 tttgccatat tcaacgtcgg caggttggtt ccggagaagg ctcagaattt gttgatcgaa
      841 gcagcggcac aagtgactgc attaagacct gaagcgcgat tttttattgc cggagaaggt
      901 cctttccatg acgcattaca aacccaaatt atgaaaaaaa gtctgcaaga taaagtgaaa
      961 cttcttggcg caagaaccga cattccggaa ttgctccaag cagcggattt gtttgtatta
     1021 ttttccgaga cggaaggaat gccgatatca ttaatggaag ctatggcagc cggattaccg
     1081 gttatcgcaa gcgctctcga aggaatggat gagttgattg aagatcatat ttccggaaga
     1141 ttaattccat ttggtaatgt tccgaagctt gcggaaacaa tcctgcaatt aatcgatcat
     1201 ccggaactgc gtatgaacct cgggatgaat gccaaacaga cgattcggaa ttcttttaca
     1261 atcgaaaaat cctgtcaaaa atatgaaaaa gtcttcttcg ggggagattc ttcgatcaat
     1321 taaggtttcg ttatggtagc caaaggagtc gctgtcctca atgcagcagc ggaagcccat
     1381 ccttccaagc cgtcattcgt acggaccttc acccaaagga ttccgtcccg ttctttctgt
     1441 tctcccagta tttccaacat cgctccgtta aattggcttt ttacatattc cgaatcaagt
     1501 ccggggttgg cacggatcaa taagccgaca tcattcgaaa cagagacaat tccgtataca
     1561 atggtcgcgg acggggtaaa tgtaattgta ggagtcttac tcggtcgaat cgttcgtgtc
     1621 ggagtcatca ctttcgtcgg agttaccgtc ggagtataag tgatcgtata agttttcgtc
     1681 ggagtcggcg gaacggctgt aaatgtaatc gttgctgacg gagtaacagt cggtcgtgtg
     1741 ggtgtgatgc tttttgtgaa agtgacagac aaggtagcag tcggctgttt cgttatcgtc
     1801 ccggtaggtg taaaggtggc ggtctgtgta attgttgcgg ttgccgtggc ggaaggtatc
     1861 ggagtgggag taaccgtcgg cggattaacc aacgcattaa atcgttctct catgggagaa
     1921 aaccgcgcat caaggttgag attaatcata tttgtaaaat cgcacaaata taacgtcgca
     1981 ccaaccaaca ggatgccgga taacaggaag gtcttaaatt gcggaacaaa gacttgttta
     2041 aacagaaaag ccagcaaact tgccgccaaa ccgattaatc cataaatgac actggtccgg
     2101 atacaatccc aaagcggatc aaaatattgt aaaccgatgc aaaaaccggc caaaccgatg
     2161 ggcagaagga ctccggccat tatcccggag ctgagcgctt gcggatgttc cgatcctttt
     2221 acgataacaa ccgccgctgc gaaagaacaa ataataaccg tgagaatcat cagaccggca
     2281 ggatcggtaa aaaaaacaaa gtcatccgcg aacgaagcca ccggtgtaga accggatata
     2341 ttcccgaccg gttggcgtcc gaatccgccc actcccaaag agctgagaaa aaataagccg
     2401 attccgatca aaaacttcag gaaactcttc cccaaaaacg aaaaagatcc ggtcactgcc
     2461 gagagagaaa tcccgaaaaa aggccccata aacggaagga ttgccaaagt cagtaccaaa
     2521 agagctttgg agtgatataa aaaagccaac ccgcaagtga tgccggcgat aattaataaa
     2581 atgtaatacc ctaatgacgg aattaattta tgagctaagt cattcagata tatttttctg
     2641 gagttatccg ggttactgta tatgcccctt ttcaggtagc gtgctcttgc cttgttttcg
     2701 ttttctattc cgttcacaca agacctcaat cctcggattt caatttgctg cttcagtttt
     2761 tacgatgatg ttattataat acacaacgaa ttacagtttt ataccgataa aaggtaaaaa
     2821 acgattcaaa attcattaaa aaacaggacc gaagaagaga attgaatgca aatgattaca
     2881 tatcgaaaac ccaatttccg taaaaatttt gtattctttc tctcaggttt gattttatta
     2941 ggaatctcgc tttttataca agggtgtaat acaccggtaa atccgacaaa agaacagctc
     3001 ggaaaaatga ccgaatcggc gaaccaacag acatcggtcg cttcccccac tgaagtcctc
     3061 ccttccgcaa caccgatacc ggatcagaag aaaataataa tcgtcagaac tccggatgcc
     3121 aatccgggaa ttttagaaat tttcgaaagc gcgcttcggc agcttcccgc cgagatttat
     3181 accatcgaag aaacagacaa tctgacgcct gcaacggtcc cggaagatgc ggacctgatc
     3241 attttcccga accttccacc cgatctgact caatatgctc aacaatttcc gcaaaagcaa
     3301 ttcgttgtcc tgagccttca gccacagacc gattcgaatg tttggaccat tcaatatgac
     3361 atcggctttc agccgttcct cgcgggatat tttgtcggcg ttactgccaa cgattggcgc
     3421 ggagccggct tactgccaag cgattctcct ttatacggag ctgccatagc ggagacattt
     3481 accaacggaa tgaaattatt ctgcgggcga tgccaatcct acggacctcc ttacgtagag
     3541 ttcccgctga ccgtcttatt acccgaggat tccgcaccgg aagcttggat cgctgcgtta
     3601 gatcaaattc aggacagctt tcccaacaca ttttatgttt ctgaggcagc tctcagttta
     3661 ccgctgctcc aaaaattgag tgaaaaacag gccgcggtaa taaccgccga aaaacctccg
     3721 gaaggttgga acggaaattg gttgggctcc gttcaaatta atctggcggg agcgcttaat
     3781 gatatcgtcc ggcgatcggc aaacggtgaa caggctgggg ctgttatacc cgaattacaa
     3841 atcgaaaccg gaattcaaca gtctttattt aatcagggga aaaaacttta tctgaataaa
     3901 ctctatgccg acttgatttc aggattggtg agcccctatg atccggtacc gcctgcttca
     3961 tttgaaaagt aacaaaaatc atctaaagaa tcggatttca acctttgaac tcttccatag
     4021 ttacatgatt ttgaccgctg atcccttccc tcttgatcac ttttccgacc gttctgagta
     4081 gaattttaat atccaaccaa agtgaccaat gatcgacata ccagacatca agccggaatt
     4141 tatcttccca ggtgatggca tttcgaccgt tgatctgcgc ccaaccggtt atcccgggag
     4201 gaagcaagtg ccggcgtctc tgttcatcgg aataaagagg caaatattgt atcaacaggg
     4261 gtcgcggacc caccaaactc atgtcccctt taatcacgca aaaaagctca ggtaactcat
     4321 ccagactggt tgaacgtaat ttctctccaa aaggcgtcag acgatctgcg tccgccgccg
     4381 gttttccatc cgcatcatag cgatcgatca ttgtccgaaa tttgtaaagc gtaaagattt
     4441 tttcgttcag acccgggcgt tgctgtttaa aaagtaccgg cgaaccgatt ttcatccgaa
     4501 ccaagagcgc aacaaccgcc atgacgggca aagcgataac cattccgatc agggtcacga
     4561 tcaggtcaaa aattcttttt gaaatagggt atcgtccgat tttcatatta caatagacta
     4621 cgaatttaaa agtcatcaat caactttttt gctgactctt atcttactac aggaagcggg
     4681 atttttgatt atggagaatg ggaaaatccg ggtattttcg ggagcacgtc cgaccggcag
     4741 acagcatttg ggaaattatc tgggagcaat tcaaaattat gtcgaattgc aaaaatctta
     4801 tgaatgtatt tactcaattg tggatattca tgctttaacc accgttgaaa cgacgaaaga
     4861 tctgaaacaa aattgcctcg agatggcttt ggattggttg gctgcgggag tggatcccga
     4921 aaaatcaatc atgtttatcc aatcccatgt cccgcaggtt atggaattgc atacgatgtt
     4981 atccatggtg gtcccgatgg ggaaactgac agaccttccg accttcaagg aaaaggtccg
     5041 tatgcatccg caaaacgtga actatggact tttaggctat ccggttctga tgagtgcgga
     5101 tattctgtta tataaagcgg agttggttcc cgtcggaatc gatcaggcac cccatttgga
     5161 atttgcccgc gaagttgttc gttctttcaa ttttcgctat aacactcaag ttcttgttga
     5221 gcctcaaatc aaagtcacta cggtaccgaa agttatggga ttagacggag tgaacaagat
     5281 gagtaaaagc ttaaataacg aaatcgagat tgcagccgac gatgaaacca cccgaaaatt
     5341 ggttatgaca atggttaccg acccgcaacg aattcgccgg acagatccgg gagatcccga
     5401 tgtgtgtaat gtattcagtt tccataaaat gttttcctct gacgaagagg ttgaggaaat
     5461 aaaccgggaa tgccggatag ccggtatcgg atgtgtcgat tgcaaaaagc agttggcaaa
     5521 aaatatcaat gcttctctgg aacccttacg atcacgccga aggaaatttg cggaaaaacc
     5581 cgatcaggtc tgggaaattt tatatgacgg tgcagatcgg gcgcgaaaga tcgcagatca
     5641 aacaatgttg gaagttcggg aagccatcgg gatcccgatt tatcgtgaat tttgagtatt
     5701 cacaatgaaa gaagcagctg ttcggatcga aacagctgct tcttttgaaa ttttattgaa
     5761 gaatttcctc tatccggaac aatgacaatt gctcatcgga ttgcaaaacc gcgagatatt
     5821 tcccatcagg tgaaaatgag atatccgcaa tacggatcgg atccgaaaca ttttggtctg
     5881 atttaaccat ctgttctttc caagtaacct ttttatcggt taaatctatt cgataaaggc
     5941 tcgtggaatc cgctgccatc agaaatttcc cgttcggatg atatgcgatc gccccaatcg
     6001 gttgttcacc gatttcaatt cgactcaatt cctctccgct ttgagtatcc ataaaaatca
     6061 gattattttt tgaataatct tcagagggag cagccacaac aagttctttg gaatcgggcg
     6121 aaagacggta tacatcaata aaatcttgaa attctatcgg agatccccaa ctttcatccg
     6181 cgatattctg caatcggaat gacgctcggg cgtgccataa aatgaatttt ccatccggag
     6241 aaaaagttgc tccgtagacc ggggctgcgg tctgaaaacc ggttaatttc ttcatgagat
     6301 ttccgcttga aatatcatac aattccaccg cgatttcatc caaactggga acaagaatct
     6361 ccttatcgtt ggaacaatcc gcacccatcg caggatagtc taatttaatt cccgattgat
     6421 atttcaccga accggcttca taaaaaagta attgccggtc ctctttaagg gtcacaatga
     6481 cattttctcc cggacaataa tcttgaataa actgatcggc tgatgccgca taaaattgag
     6541 attgcggatc caattcggga accgggtaac gctttatgga acggctccct atcaaaaaga
     6601 tattttcgga atccgcagac caagatattt tcgtaacggg aggaatttcg tttgtacccg
     6661 attcaatttc agttgatcct tccggagctt ggaagccggc gcaaaaaata acgatgaaaa
     6721 gaatgaaccc aatcgaaaag attgtttttt tcactccttt tataatatcg ggaaggtcga
     6781 aatgaatggt taccatagat taaattttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnntc caaaactttt tggcgtaaat ctttattttt attttgagtg tcgatcagat
     6961 ccgtccgata tttcgccaag ttttcttcca attctcccaa ttctttttga tgaatcgatt
     7021 taatgcgttt actcttaaac caactcggta aagtcagaat ggaaataatg atcgcaccca
     7081 ccgtaagagc tatcagcagt aaaagcccca atggactttc gaatgtccaa ataaaaaatt
     7141 tcagactcac gacagcggta ttctgcaaag caaacatgac cgaaagaaca gcgataatca
     7201 gtgaaaatat catcacaaat tgcataggac cctccttttt tataattgta taggattttt
     7261 ttattctttc acaggttccg aaaaaactca atgaaagaat cggcaataaa ataccataaa
     7321 agaataatgc ttataattaa accatgtcga agactcatac ccattatgtt tgccaacaat
     7381 gcggaagaac caacccaaaa tttttgggaa ggtgccccgg ttgcggcagt tacggaacta
     7441 tgatagaaga gatcattccc gaagaaaacc cgcaaagcgt ttcgggaata cttcacaata
     7501 acccgattcc caaggcctta cgcgatattg atgaaaaagc cgaaccgcgt gtggataccg
     7561 gcatcggaga actttcgcag gtattaggcg gaggaatcgt tcccggttcc gtcatattaa
     7621 tcggcggtga tccgggaatc gggaaatcga cattaatgtt gcagacagca ttacaccttt
     7681 cccagaaagg gttggtattg tatgtgtccg gagaagaatc cgaacgccag atcaaaatgc
     7741 gggcaacccg tttattggaa aataattccg ttatccgtga aatacccgaa aaattgtact
     7801 tggttaccga aacaaacttg gatgtcatat tttcacatgt tcaatcactc aaaccggatt
     7861 atttaattat cgattccatt cagacggttc aacttccgat tttggagtca accgccggat
     7921 ctctcacgca ggtccgggaa tgtgccggaa aactacgcga attggctaaa accagcggga
     7981 ttacggtatt tctgatcgga catgtcacaa aagaagggat gattgcaggc ccgagagtat
     8041 tggagcatat tgtcgatacg gttttatatt tggaagggga tcgctttctt tctttaagat
     8101 tattacgttc tgtaaaaaac cgcttcggtg cgacttccga agtgggcgtt tttgaaatgg
     8161 ttgaaagcgg attaatcgaa gtgaaaaatc cctccgaact ttttttggcg gaacggatgg
     8221 tcaacgctcc gggatcggcg gttgttgcaa cgatggaagg cactcgaccg atcctcgtcg
     8281 aaattcaagc tttgactaac ccgacttctt tcggaaatcc gcgaagaacc cccatcggcg
     8341 ttgatatgaa tcgattgttc ctgatttcag cggtaatttc acgcagaata ggcttgcgtt
     8401 tgagtgaaca ggatatattc gtgaatgtgg tcggtgggat gcatatttcg gaaccggcag
     8461 ccgacttggc gatcgccgct gcaattgtat catcggtaaa gaatcagcct attcgagcgg
     8521 atgccgttat gattggagaa gtcggccttt ccggagaatt gcgcggtagc ggaaagttgt
     8581 catcacgctt gaaagaagcc cgaaaattag gtttcacgac agcggttatt ccgaaatcaa
     8641 tccatcataa agaaaaatac cccgaaggaa tgaatgttaa agaattccgg acacttcgag
     8701 aagctttgat gtttattttg cttcccgttg ccgaaaagga aggaacatca tccgattcgg
     8761 atattattaa ctgaaaataa aggacccatt atggaaaaaa caatcgaaac aaaagttcgg
     8821 gatctttgta ttcaaaatcc tgtaaaccat gtgcggaaaa atcatgcttt ggagcatgcc
     8881 acggttcata ttctcgaaaa gaaattcccc gataaagaat ttgccggata ttccgttgaa
     8941 agaggttttt ggattatcgg gaagttatcg attcaggaaa ttcaagaggc ggctgatgcg
     9001 gcatttgcaa ggctgtcaca cggagaaaag gatctcgctg tccatccggg atgcggaacc
     9061 aatttggcga ccgcttctct tttaacctcg gtcagtgctg ttttaacttt tatcggaaca
     9121 aaatccgaaa aagacaggtt aaaaagattt tcttctctgt tgctgatttc aagcatggca
     9181 attcaggttt ccaaaccgtt gggatttgcc attcagagga atctaaccac cgatcccgat
     9241 atgaacggaa tggaaatcag cggcatcgat acgggacaaa tccgagagta tccttatttt
     9301 ttcattcgga cagtttatca gaaaccctgt gaatcctctt tatgagttct tcaaaaaatc
     9361 tgactattct atacggagat gacgaagaat cgataaccga atatctgagc cggattgnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg gtcggaaacg atacgcgggc
     9541 agccaaacag gaactcgaga aactgcttct atatgtaaat tacggcagac cgattgacag
     9601 tatggatatt caagaattgg ttaccggttc agcatccgtc agtatttttg atatggtcga
     9661 tgctttggca tcgggaaatg caaaagaggc gttccgaact ctggacttat tgatgcaaga
     9721 tcaggaaatt ccggttttat ttgccatgat tatcagacag tttcgcttat tgatccaaac
     9781 ccgtgaaatt atggatgaaa aaggaaatat cgcaaccgtt caagatgaac taaaccaaca
     9841 tccgtttgtt gccgaaaaac tctggcggca ggcaagtcgg ttccgattcc ttcaattaaa
     9901 atctatttat cggaaattgg ttgaaatgga ttttgcattt aaaacaagcc aaccagattc
     9961 gagagccgcc ttggatgtac ttatcgccga tatttccctt atggtacagg attaagattc
    10021 ttttcgttag gtttaacaga tatatacggg accttccagt gtccgctgcc gtcttctgtc
    10081 tgatttcccg tcggttcggc aatttcataa accggattat ttatctgaac aacgtggacc
    10141 cagcttttcc ccggtttcag atgaaacgca tccccgtcag cctctaaaaa gcgaatcggg
    10201 cgtaactttt ggttttgaat ttcatattct tctgctttgg ttgtccaaaa agcctgataa
    10261 cacaatccat cccgaaaaat taacgccgga ttctttttaa cataattgaa attaacggtg
    10321 aaattaacgc cgtcttcaat taatgaatga tccgcaaaga gcaaaatgac attttgggca
    10381 gtaatttgct gattattaag aacatcgata tccttaaccg gattcggatc ggaaaccgag
    10441 ttttgagatc ggtgatataa accgtcggct tcgtcaaatt cccaaagaat ttgattaaaa
    10501 acggcataac ggatgaacag cgtctttccc gaccggctgc cttccggaat cgccgctgaa
    10561 aagggaagcg atattccctc tatatcggga gaacccagtc gctttttcca tttttccata
    10621 atttcaatga accgttgcac cggcaaatcc ggatattttt ccgaaccgga ttcaccgtac
    10681 caagtctcaa ggttagatat tcccgctttc ttgacaagat cgggatttcc ggcggttatc
    10741 aataccgcca tatattgctt tcgtagcgat tctaatccga gaacagccga agatacggaa
    10801 ccgatataag gtgtaacgcc ggttgtcgat tccggaatac tcccataaaa cagagccatc
    10861 ggtcgcgatt ccccgtctcc ggagtacatt tcaaaaaccc aaggagcaaa gctcaatccc
    10921 gcggaagatc tgaatttatc cggaaatttt gtgtaaggaa taataaacgg aggataactt
    10981 aaccaattag gatcagtctc gattccggtc agcggattga tcaccttttg ttcctgatcc
    11041 gcccaaacac tggaagaaca gcctgtaatc accgtgaata caatcaacaa aattgtcaaa
    11101 atcttatggt ccattttggt tctttctatc atgaaattaa ataaccttcg ctcgcatcta
    11161 agaattgtac aacagattct ttaaacacaa atacctccga tcaatcggag gtattttttg
    11221 ctcctcgacc tggattcgaa ccgggaacct aacggttaac agccgctcgc tctgccgttg
    11281 agctatcgag gatcaacgcc taaattatag caacttttaa aagcctgtca aggacgaccg
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgaggatca acgcctaaat
    11461 tatagcaact tttaaaagcc tgtcaaggac gaccggcgat tctgtgttgt tattcagcaa
    11521 taatgtcatc ccgatgaaat acaggcgatt ctgtcatccc ttccgaaaaa taaaggatcc
    11581 ccaacggaat gaaaatcaaa atggatatca aagaagcacg acgaatcaca gttattgaac
    11641 aagccataga aaagaagatt agcgaccgag aagctgcaca gctattagag tggtcggcag
    11701 catatggtaa atgatttttc acttcaccgc tgcgagaaag cgcttatctt tttttctgat
    11761 cgtatcggaa gtacaattaa atcatcagca gaccggataa taaataagtc atttttcttt
    11821 cccaatgaaa gtcgaggcta ctatggcaaa agaaaaaaag aagaaaaaga agaaaatcga
    11881 tcagaaaata ggatcgggac aaacagaact atcaaatgaa aaaaaggaaa acgaaaagaa
    11941 aaaagacgta atttccgcaa taccaacctc gaaaagtaca agtatcccgt gcggtatgcc
    12001 ttacacaaaa cctcccctca gcaaaaaaga atatgaaaaa gaactggaaa aattgcaaat
    12061 cgagctcgta aaactacagg ggtggattaa ggaaaaggga ttgaaagtcg tcgtaatctt
    12121 tgaaggacgg gacgcagccg gaaaaggcgg agtgattaaa aggattaccg agttactgaa
    12181 cccgcggatt gttcgtgtcg tcgccttacc ggtcccgacc gaaaaggaaa aaacgcaatg
    12241 gtattttcaa cgatatgtcg ctcaattgcc ggcggcggga gagatggttt tgtttgatcg
    12301 aagttggtat aaccgggcgg gagtggaacg ggttatggga ttttgcaccg aagaagaggt
    12361 aacggaattt ttccgcagtg cccccgaatt cgaaagaatg ctgattcgct ccggcatcat
    12421 tctgatcaaa tattggttct ccgtaagtga cgaagaacag gaaaaacgat tccaggatcg
    12481 gattaatgat attaccaaac gttggaaact cagcccgatg gatttggcgg ctcgcagcaa
    12541 atgggtggaa tattctaaag caaaagatga aatgttttat tacaccgaca caaaacaatc
    12601 cccctggtat gtggtcaacg ccgatgataa acagaaagcg cgcctgaact gtattcatca
    12661 tctgctgagc ctgatccctt atgaagattt gactccggag ccggttgttt tgccgccccg
    12721 acagtctgat gagggatatg tcaggccgcc tatcagtgat cagacattcg ttcctgaaat
    12781 ttactgattt atactaccgc atatagttgc gaaaaaaata aacgcaaaag attccgtttg
    12841 tttaaacaaa tggagtcttt tctttctcat ccgagccgtc cgattataaa aataaacgat
    12901 tgaaattatc gattgaacaa atattttcat aattggcatg aatgatgcac actttatgat
    12961 taataagatt ttaaatactt acatcaaata aggatgcagc ttatgaacgc ccgaaaaatt
    13021 ccttttctcc tttttctttt tgtcatattc ctgatccctt tttccgctga agccgccggg
    13081 gatcagacaa acccaaattt atttccggtc acagttgcaa aaaatatcga tccgggactc
    13141 attgatgcga tcaatgaagg agattccgga ggcgaaatcg ggttggccct tatcggaagt
    13201 gacaccacag taggatacta taaaaaatgt tcgaccgatg catactttta tttcattgtt
    13261 gcaaacaaca gcggtgcaac tgtgggtacg gttctgccga gatgggctaa aatagacggt
    13321 cgggcatatt ttaccgacag tagcgcttat ctctgcaagg aacttgcagc aggcgctaca
    13381 ccaagtaata atttcgccga tgccgacagc ggatcggctt gcattcttca aaacaatcaa
    13441 ctgagggtag gagccggtaa agccgtatcc gttcgcggca aaatcgtcaa tttttcgcat
    13501 gagctgacca gaggaaataa gaaggattcc gatccggaat atttgcatat caatttctat
    13561 gttcagatta acaatagtaa aaaacaaggt acgggggctt tggaatccaa aggaagtctt
    13621 tgtgacggtt taaattttct gccggttacg ccacacaatt atgtagacgg cggttatttt
    13681 atcgatccgt cgggaagacg ggtttgctcc atgaattatt tctttattgt tgaaaatccc
    13741 gatctatttt actctacggt tgttgaactt ccgcgctggg tagaactgga tgaacaaaga
    13801 ttcaatgtcg gatgtaaata tctttatccg gggtgtaaag tttcaggctt ccgccattat
    13861 gcatgggcga tactggatga ggatgataca tatatcggcc cgaaatatta tttttgcgat
    13921 caagaccggg cggtagggaa taattgttat gaagaaaatt ccttatggtg gctgcgggtt
    13981 cctcccaata caaagatagc cgtccggggg atggtcaata atttatgcaa tgtccctgcg
    14041 gcaaacccgc tgcaaaactc agaacttgta gtcggtttcg atgtcagcga tgccggttcc
    14101 aataaatggt ggctggatgg cattgttgaa ggaaccgagt caaaagacct ggcaaacaac
    14161 tcgaaagata tcaattttat agatgaacct tcaagattag aagatcggga taaagatgag
    14221 cccgttgacc gctatttatt ggaagatcca tccctggttg atttggaaga ggaagcggca
    14281 cagccgataa ttatcgaaga gacatcggaa ccgtaaaatt atagcgtcga taacttttta
    14341 aaaggaggcc ggatcatttt tcggtagcct cctttttatt taatgcattc gagggaaacc
    14401 ctttcctaat tttgttgatt tcggttaaca gtctgtggta cgatcaaatc atgaaatatt
    14461 ttcgtttttc ggctttcttt tttattcttt tggtaaattt ctttatggct attaaagttc
    14521 tcgctcaaga tgcagagcaa acttatcggg taaaaaaagg agacactctt tcggaaattg
    14581 ccgcccgatt caatctgagt caagagcagt tgatcaaggc gaatccccaa atcgatcccg
    14641 atttattaat ggtgggagaa gaattacaaa ttcctcccgc agattcaatt tcttataatc
    14701 ggtatcttca aaatcgatat aacgaatacc ttgcgattca gcctgtggat tgttatccga
    14761 atcccgacca aaccgctgaa tgtttgacac aaatcaaaaa tatcggcgac cgtaccactt
    14821 acaatatccg actgatcgcg cagaccacag attcggaagg aggaattcgg gaaagtgtca
    14881 gtagtccgat tgtcaaccaa gtacttccgg gtgaatcgat acccgtttta ttccgtttcg
    14941 ataatcgatt atcggatacg gacactgttc aatgtacaat tgattcgttg gaattatcga
    15001 aagatacgtc tctgtctttg cgcttggatg agtctcaatg gtcaaaggaa atagtttttt
    15061 catcggacaa aaaaagcgca aaagtgattc ttcattttga acaagccgct atggaaaaaa
    15121 cgaaatcaat cttaatcgca gcttataacg aagacggaaa agttgtcggc atccgcggaa
    15181 aaacaaattc ggttgaacag gatctgtctc ttttcgttta ttccctgagc ggaatgattc
    15241 aggatatatc cgtttgggtt gaagcctatt aaagattaaa aatgacaacg atcttagtta
    15301 ccggtgcaac cggctttatc ggatcagctc tttgtcggga actatgcgag agaggatatc
    15361 aggtgatcgg gttccatcgt tttagcagtt cattgaaaat gatcgaagga ctgcccatcc
    15421 aacgggtcat cggagattta accgatgaaa gttccataga aaatgctttt tcctatcatc
    15481 ccgagattgt ctttcatttg ggcgcgcagc aattttccgg caggaatctg acccgtctga
    15541 tggatgtcaa cgtcggagga acacgaagga tttttcaagc ttctttcaat gctcacgtca
    15601 aacgaattat cctgatgagc tctgccgtta cgatgggaac ggctgagctt tcaccggaca
    15661 gacaccgttc accgataccg gtcggtgaaa cacacggatg ggatcggact ctcgaaagtt
    15721 ggccgttcgc ttggtcaaag ttattggcgg aatatgaaac gcagtgggca atcgcttatg
    15781 ggatggatat cgtcatctgt aaccccatgt tcgtgatcgg accgggggat tactatcgca
    15841 gaaataccgg tttgattgcc cgatttcatg ataaaccgcc caaatttttt atcgaaggcg
    15901 gcatcaattt aatccatgtt aatgaagttg tacaaggttt gattcaggcg atgaattacg
    15961 gtgaaaaagg ccggcgttac cttttatccg gaacagaccg gtcgtttcaa caattctttg
    16021 gcgatctggc aaaattatcg gggactcctg cacctcggat gcaactcccg accagttttt
    16081 caaatcgttt gtatgcatat cggaaccgac atccggactt attccccgtc gaaaaaacgg
    16141 atatcaatct gcttcgaatg gcgggaaagt acttttatta tgacggcaag aattccagat
    16201 tggcgcttca tcttcccccg ccgcagccga tagaaccggc attacaggag actttgaatt
    16261 ggtttcggga aaacaaatga atcgggaaac cgtgtgtctt ttaccggaaa taaaatccgt
    16321 agggggaccg gctgcttttc agcaacggtt tattacagcc gccgccaaac agggtatcac
    16381 tgcccatttc gattcttcgc gaaaggatat cggtgcttat ttggtaatcg gagctccgcg
    16441 aaaatttatt ccgacccttt actctgcacg acaacggggg attccggtta ttcagagact
    16501 caacgggatg aactggattc atcgtgtccg accgtccggt tggttgtacg ggaaaaaagc
    16561 ggatctggca aacaaccgac tttctttttt cagaaagcac atcgccaccg gaatagtcta
    16621 tcaaagccct ttttgtgaaa cacattggaa tgaagtttac ggagttttat cggataagcc
    16681 ttcccgaatt atctataacg gtgttgattt aaacgaattc aaaccgattg ttcccgaatt
    16741 ttcgtccgaa aatatcgaaa tcttaattgt cgaagggaac cttgaacatg gttccgagca
    16801 aaatctggaa caagcattat cgttatcaat ggcattgtcc cgtcgaactt cacaaaaagt
    16861 gcatttaaga atcgccggaa acgttcccga agctattcga gaaaaagttt ttgagagaat
    16921 gtcggattca accgccgtag tatccgtcga ctttttggga atcatcgaaa aatcaagatt
    16981 aatcgaactg gaaaattcat cccatatgtt gttttcggta gaactaaacc ccgcctgtcc
    17041 caattcagtt atcgaagctc tggcttgcgg attgcccgtc atcggatttg atacgggggc
    17101 gttgaaagat gttaccggtg tcggagggat tattgtcccc tatggaacgg atgtttggaa
    17161 attggaaaaa ccgatccttt ttccattgat cgacgcagcg gaaaaagtct accgggaaaa
    17221 ccaaaaattt cgtaaagcgg ctcgcgaaca agccgaatca catttttcaa tcgataaaat
    17281 taccgaagaa tatttatcgt ttttattcgg atcataagaa aaatcaatct catttcgacg
    17341 aaacgacaaa aatggaatga ccggtaacag ataaaaactc ttttaaccct tcacggctaa
    17401 ttatgacggt tgctgtgttc accaaaggat gtaattggac tgactcggct tgtaaaactt
    17461 catcttcaat gattacacgg atgcgattga atggatcatt gactaatgcc aaaagtgaaa
    17521 ctgctccggc ggtaataccg agaaagtcaa ccaaatttct ttcatttccg aaacgaagcc
    17581 gcgaatcatt catgattttt gcgagttgtt tcagattcgc ccgttgattt tcatggcaga
    17641 cataaagata atgccgtgtt tcatcgttat tggaaagaaa aagatttttc gctcgaaaac
    17701 ccggcaaatt tgcggtataa acatccgctt ccgcactggt ataaacagct ggatgatgaa
    17761 aaacagtata agaaataccg tgaaccttca aataagccag aatagattct tcggtataaa
    17821 atgatgaagg cgaaaacgga cccatccgaa actatacgga agttgataga atcagatttt
    17881 tccccttcgg gctttcccag ggataaatga tccaagcatc ggtaatcgcg gcataaaaat
    17941 ccggtttttc ttcgggaaac aaattccttc gatggttata atgcaacaca cacgtgaaag
    18001 gcatcccccc ggcaccgctg acgcggttgc gtaccgccga aatacaacga ccggttcccc
    18061 aagcacagcc gacgattaac acttttttct gttcgatcaa cgaattatcg gggaaaaggc
    18121 ggatagtcgg ccacgaaata aatcgcgggt cactgcgctg tttttccaac tcaaattcct
    18181 tgggagaatc aatatccgtc agataaatat cttggatccc taatgcttcc gacaaaatcc
    18241 cgccggggat gattccccct ttttggatca tgagaattac atcgaattca tcatgaaatt
    18301 gggggattaa atgatcgatc aacttatcga cttcaagcca ggatattaat tcttttcgca
    18361 tattttattc aatccagtgt gaattatagt tttgaaatgt gaattaagct atgaagattt
    18421 ttattctata aacaaggcat ccattcattg aaatttaatc tttccgttct gtcaatatta
    18481 aaaggcattc agccgtcata tcgattcgcc gaaaattcga agaaaatgat cagcccatgg
    18541 tttcggtatt ttgttgtctt caaatgagtt gtattcggca taagtctaaa ctataataag
    18601 aaaggtaaaa atattaatgc cggaaagatt cattcgacca tgaaagccac aaggttcatt
    18661 tggtttatca tcatgattgc agccggaatc gcaatcgggc tgtattatac ttggatcgtc
    18721 catcccgtca aactggtaga tgcaacattt tatgatctga ggcaggatta caaagctgat
    18781 tatgtgctga tgactgccga gatatataac gataacccca atctctttca gactatgatt
    18841 cgacttgatc gattgttgga agaatctccg gaaattgccg tcgcgaatgc cattcgaaat
    18901 gccgatcaac tggggtactc tgaaacagat atcgacatgc tttatcgctt gaatgaggca
    18961 atcggcggaa atgcgattga aacccccgat gccgaagata tttttatcga atttgatccg
    19021 cagacatttg taaatacgga aaccgctgtg ccgatccggg aattatcagc gccggcagaa
    19081 atgccgacgg aagacaaccc ctttatagat caatccctgt cacagccgac gctcgtccca
    19141 tccgatgagc cgaatccgtt cggagaaccg taatggtaga gacaggacgg aaaggaccct
    19201 attacttatt aaccggtctg atcatcggat taattctcgg gatattttac ggttgggtca
    19261 tcaatccgac ccgttatgtg gatatttctc cggaagcact ccacccggat caaaagaaac
    19321 aatatctttt attggtagcc gaatcctatc aggcaaatga agatatcggc cgatcttatt
    19381 acagaatcaa acagatgacg aacccggtga atatcgatga gctgcgggca atgctcctcg
    19441 aaatggaatc ggacccggat tatgccgaac aattcgaagt ggtccgtaat tttataaatg
    19501 atctggacag ctatctgaaa cagcaaaatc cggataacaa aatctccaca caacaggttc
    19561 aagggttgct aaatccgagc gatgcccggg aaaaaggaac cccggcaacg gcaatagatg
    19621 aacaatttaa taaagaaacc gcaaatccgg aaagctttta tgtaaattca aattaaccca
    19681 aaggtataat taaccgaccg aacaaatctg agacatactt cacggaggat tcgatgatta
    19741 atgacattat tacagattcc gaaaacagaa tgaaaagtgc gatcaatgtt ttacaggaag
    19801 atttagaagg gatcaggacg ggcagagcga caccggcact ggttgaaaag ctgacggttg
    19861 actattacgg gatggagaca ccctttaatc aagtcgctaa tatcagtatt ccggacgcaa
    19921 aaacgatctt aattcggccg ttcgaaacaa atttgatccg tccgatggaa aaagcgattc
    19981 aagcatccga actcggtctg actccgagtg atgacggtaa atcaattcgt ttgatgttac
    20041 ctcctctgac ggaagatcgc agaagagatt tgatgaaatt ggtgaataac cgtcttgaag
    20101 agacacgagt agcgattcgg aatatccgcc gcgataccat aaaagaactc aaagatttgg
    20161 aaaaagcaaa agaaatttcg gaagacgaac tgaaaaaagc cgaagaaaaa atccaaaaat
    20221 tgacggatca atggattacc caagtagatg aaatcgggaa acggaaagaa gcggaaatca
    20281 tggaggtctg accgatatgg cacagacaaa attgaacatc gagccctcaa gaattccgcg
    20341 acatgttgcc atcatcatgg acggaaatgg gcgctgggca aagaaaagag gattgccaag
    20401 acttgccgga catcgccaag gtgtcgaaaa tctcaggcaa atcatcaaag cctgtgttga
    20461 attcggaatt caatatctga ccgtctatgc gttttcaaca gaaaactggg gacggcctga
    20521 ggatgaatta agaggattaa aacagatttt caatgatgct ttctctaacg aactcgaaga
    20581 attgatcaag aacggtgtgc gaattttaca catcgggcag cgggatgaaa tcagcccgga
    20641 ccttctgcaa aaaattgatc gagcggtaga aacctcaaaa acaaatgaca aattaattct
    20701 gactgtcgcg ttgaattacg gcagcagaaa tgaaataatg cacgccgtac gaaaaattgt
    20761 cgcttccgga atacctccgg aggaagtaac ggaacaggtc atcagcgatt cactgtttac
    20821 cgcggggacg cctgatcccg acttggttat ccgcacatcc ggtgaacagc ggctcagcaa
    20881 ttttttgttg tggcagtcag cttattcgga atgggttttt ccggaagctt attggccgga
    20941 ttataatcga gaggaattaa taaaatcact ggaagaatat gcaaagagag atcgcaggtt
    21001 tggaaaactc tcttcaaaat aaaacatgga taatttaaaa acccgcgtca tctcagcagc
    21061 tgttcttgcc cccttgttca tttttttgat ttatctggga ggaattcctt ttctctttgc
    21121 atcccttcta attatcggca tcggctcctg ggagttttgg cggatgtaca ataaggaaca
    21181 gtcaaaccgt tccagtgcgg ttttactgat cggcttatcc attctgacag gggcatcccg
    21241 ttattttttg gattttacaa ttgcatcgtc tgtgcttgcg ctttgtatta tgatcgccat
    21301 gctttgggga aacatttcct atgaacggaa aaatcagaaa gccgcggatg ggtttgcggt
    21361 gatgaccacg ggaattgttt attttggctg gatcggcagt tacttgatta cacttcgcca
    21421 atcggaaaat ggtttttggt gggttattat ttgcctgctg ttagtctggt tatctgatac
    21481 cggggctttt ttcgtgggca gcaagttcgg aaagcatcaa atggcacctc ttgtcagtcc
    21541 tcacaagact tgggaagggt atttcggcgg cgttttatcg actttagtta ttggaattct
    21601 tctgtttctg atcattccgc ctgtttcaaa gatcctcaaa ttcccgcaaa tgattctgtt
    21661 ggcactcctg atcggtttga tcactccttt cggggacttg ggagagagca tgataaaacg
    21721 tacctttggg tttaaagatt cttcaaattt aatcccggga cacggcggga tttttgaccg
    21781 aatggacacc gtattttgga cgatggtaat cggatattcg atgtatacct taattaattt
    21841 gggtttaata taacttttac gacacccgcc ggcgcatttg cattcacaac aacctggctg
    21901 ccattcatca agccgtttgc cggaaatatc tgagatcccg attgcatata tgtctccgga
    21961 tagaatacgt cggttgcatt ttggactgta acataagccg acacggaagc gggaacaaaa
    22021 atccgaatat ctccgctttg tgtactgata ttcaccggaa cacttgcggc ataaggaagc
    22081 atcacatcga tatttccgac ctgggatatt aaattgacgc cggctacgtc taaatcgcgc
    22141 aaatcaacca tttgatagcc attcgccata tttaccatta atacaaccgg tatttgtcga
    22201 ttaagctgta aatcccaaat cgcttccgaa gaaccggttt gagaagcggc agttccgttc
    22261 atcatataga tggaacccga atcactttga tcgatcttgt cattgaagga ttcaccctcg
    22321 gcaagtcgaa tcgtcccggt cataagatgg ccgttcattt ccttactctt cagttgaaat
    22381 tttccggaag gaactttgat ttggaatgtg gctaaggact gagaagttaa cggataatcg
    22441 atatttttta ttccttcgga atcaattgag ccgatccggg ttgagactag ctcactgatt
    22501 tttgaaaaat taaacgactc ttgaagactt gaaacagaaa attcaccttt attgataaaa
    22561 gcaacgtaaa gaattgcacc gataacaata aatggaataa taatggaact cagagatttt
    22621 ccgctaaaaa gcatatcaaa tccatagacg atcaggaata aaggccaaaa agtcataaag
    22681 atttcccatg cttttgtatt tccgtttaaa ttaagaagga tcaacgctac cccaaggaag
    22741 gaaagcacaa cagctccgaa aagccgcttc ccatcaaaat agaggtccaa tccggctgca
    22801 ataataacaa tcggccagaa tttggagagc agattttcct gcgaattgcc gagaactccc
    22861 gatgcctgaa gctgaaaaat cagaccgcaa agaataacaa taaggggcag aataagcgag
    22921 tgatgatttc tgtttgtcga ttccatacaa cagatatggt gcaacaatca tgcccttgtc
    22981 aattatgaaa gagacaatta cagatattcc gtaataatct ttctaatgat catgttttcg
    23041 taaaataaaa ctgaaaacta aaccacacgc gctgatgacg atcagcatga ttccgatcat
    23101 ttttcgataa ggtttgactt ttattaatat ctgtgaagca ttccattcgg ttaattcggt
    23161 ttcggtaggt tgaggagtga caaccggttg tccttttatc gcagccgaat ccgtcgtaaa
    23221 tgtcggtcgc ggggttcgtg tcgggggacg cggagtttcc gtaaccgtcg gagtcagtgt
    23281 ttgcgtgaat ttcggacgaa tcaaaagatc ctgcccttcg ataatcaccg gatttttaag
    23341 atccagatcg ttcagattcg ataaagtatc ccaatcggta tgataagcat aagcgatttc
    23401 ggagagagtt tctccctcct gaattttatg aataatagat ccgtccggat tcggtgtcga
    23461 tatgaggatc atttttgccg tcggcgtata agacaattga ggtttcgggg tgcgctgatc
    23521 gtaggtcggt aagggagtat acgcttcggt tccactgtcg agccggccga gatatgcaac
    23581 cacatatcga tgtcggccga catccaaaga ttctcccacc ccgatatgaa caacgtcttc
    23641 gttcaacaaa gcagctcgat cttccgcctg gtttagccaa atattcttta caatataact
    23701 cggttcggta tcggaccaag acatagcgat acattgttcg atttgaaaag acgatcctcc
    23761 cccatatccg aaatcggctg caatcgattt tgcggattcg ttattcagcc ctttttcggt
    23821 caactttccg attttcgcca gataagctgc ctgttgatcc gctgcattcg caaggctttc
    23881 atcccaccga agagccggaa gatttttttc ccgccttgct tggttgattg tttccatgat
    23941 ttcttcacga aaaagccttt cggtcggaat aacggttgga gtaagagtta tcgtttcgat
    24001 tcgaaacggt gaaagtgtat ttgaagaaaa aatgaacgga aaaacgaata aaatcagcaa
    24061 gcgaatcgtt ctcgttaagc gacaattctt tttcatcgcc tgaaaaatcc gaagaaaatt
    24121 gtgattaaca atccgatacc gctgaaaacc accagaacaa ggcctaatct tcgcgctgcc
    24181 ggtttatcaa aaaaatcgat atgcttaatt aacggtcccg gcgtgggtgt ggctgtaatg
    24241 gtgggggtaa gcgaaggtaa tggaggcaaa gtttgagtca tcgtagaggt cggtttcggc
    24301 gtattggttg ccggcggttt ggttacggtc ggcgtcaggg tatccgagac cggcgttccg
    24361 ccgtaatgga ttagtatcct ctggccagct ctgacgctaa cgtcattttc agccatcccg
    24421 tttaacgcct gtagctgtgc aaccgtcgta ccgtaattaa tcgcaattgt ccacagagtt
    24481 tcattttgtt gcacagtatg ataaatcgat ccatccagtt cgggggtact ggtagcgaag
    24541 gttccgggtt gaaccggcaa cgcttcgata tccacaaaac cgctgcccgc attttgattg
    24601 gccgaaaccg cagcggttac agccaaggtc ggggatttat ccaggtttgt atcattcccg
    24661 gaactgttca ccatcagtgt atagtatata cttccgtcgg gtgcggaggc cgctccggct
    24721 ccgactgtcc ccgaatcgag gccgatcatt gtataaagat gatcgggatc tgtccacatc
    24781 tgtttgacgc aataggatgc cgtcatattt aatccgccgc agacattttc cgatcgagcc
    24841 ggaatattcg atccatccgg gcgattatgg gttgtcatat tgatggaagc ctgatattca
    24901 ctctgagcct gggctaaaga tgataatgag ttgtcgacgg tataaggaga cagtccgtaa
    24961 gaagctctta atgcatttac ttccgcaatc agtgaatttg catcctgtgc atacacggga
    25021 tagataatga aacaagttgc gaatgccaag actgataaaa taaacaaaaa ttgaaaactc
    25081 aatacacttt ttctcatttt ttctgattat aacccgatct tttttattcc ttgcattcga
    25141 tacgatttat gctgcaatac cgtcgaacta ctcgaaatat tcctctgtaa tctgataaat
    25201 gttctgatat tcttgacatt gaacaattgt tctgttatat taattttaga acaattttat
    25261 tcactgttag attttgtgag acgggagtag attatggcca gaatgggaaa aatgatgaat
    25321 gatcggcatc gaaaaattat gctattcctg gaagaatatc aaaagaataa tcaattttcg
    25381 ccatccattc gggaaatcgg aaatgcgatc ggcgtaaatt ctacctcctt agtagattat
    25441 tatttacggc aattagctca aatgggttat ctggaacgta atgatcgggt ttctcgcagc
    25501 attcgaattt tgaaaccgtt tacagaagaa tcgcagaccg caaaaaacat gatttcatat
    25561 tcccggcagg aagtactccg ctacccgatc ttgggaagaa tcgttgcgag cgagccgatt
    25621 ccggtgcctg gttccgattt ggcatatttc gatccggaaa gttatattga aatcccgaag
    25681 aaaatgattc ccgcaagtga aaaagagacg gagcttttcg tcttggaagt ctccggtgat
    25741 tcaatggttg acgccatgat caacgatgga gacaaagttg tttttcgtca ggcaaatcag
    25801 gcttcgaatg gcgatatggt ggctgtctgg ctatccaaca atgatgaaac tacactgaaa
    25861 tatttttatc gggaaggatc aagggttcgc ctccaaccgg caaatcccac aatgggaccg
    25921 atttatattg atgatccgga acaagtccat attatgggaa aggtcgtgat ggttattcgt
    25981 catctgtaaa ccgcttcaac cgttcggttt agattcttca ggtacaatcg caaattgaac
    26041 aatcaagctc ggcaaaagaa aattgccatc atctcatctt tatacatgcc tcatctcgga
    26101 ggcgttgaaa aatattccca gtctctggct cgaacactga cagcggatta tgaagtccat
    26161 gttttttgta tgaatacgga atctgttccc gcggaaagtc gtgacgggga tattttcatt
    26221 catgcgttgc cttgcggcgg ttttttcaaa ggccggttac ccgttcccac cccaaaagcc
    26281 atccgccgga ccaaacaaat ttttcgagag accggatttg acttcggaat catccaaact
    26341 cgcctttatc ctttcaattt acaggcggca aaaatattga aagaattcca aattcctttt
    26401 ttccttatcg aacacggctc agcttatgta actttcggta actctctgat caatcgctta
    26461 tgggaatatt acgaacgcca tcaagcaaat atattgaaac gttactgctc ggatttttat
    26521 gcggtatctc aggcaagtct cgattggttg aatcatttcg gaatccaagg gaaggggatc
    26581 atccacaaca gcatcgatcc gcgggaattc gaaggaattc ctgccggttg gcgcaaatcg
    26641 gtcggaatcc cttcggaagc gattcttctt acgtttgccg ggagaatcat tcaaaaaaaa
    26701 ggaattgtca ctttattaac cgcagccgag cggattgaag aagagaggct tcacatcatt
    26761 atcgccggag acggagatga aacgctttta agcccttgga aacaaaattc tcgaatccat
    26821 tttctcggca aagttcctca tcgtgtgttg ctggaaatac tcaaagacag tcaacttttc
    26881 tgtttaccga caacatatcc ggaagggctg ccgaccgtta ttttagaagc cggttatttc
    26941 ggtgtccctg tgatcgcaac cgatatggga ggaatcaatg aagtaatttc cgacaataag
    27001 accggaaaga ttattcctgc ggatgatcca gaatcgttgc ggtctgcaat tctggaattg
    27061 atacgagacg aaaaagagcg gaacctattc ggatcggaat tgcaaaaaaa ggtctcagcg
    27121 gagtttactt ggcaaaagac agcactcgaa gtgaagaaaa tcatcgaatc cgtctgaacc
    27181 gcttcctatt tttcggattt ttctatatcg tttataaacg cttttattgt cagtttcaca
    27241 ggtgtggcgt taaaataatt caatcgatcg gttttaataa atcacagcgg aaatgcttgg
    27301 gagggatttt gaaggttttc gtttctggag tggcaggttt tctcggaaca ccgcttgcca
    27361 accaactcgt caaagaagga aatgaggtta tcggtctgga tgatttttcc gccggcaatg
    27421 cggaaggctt agatccggct gtccggttag tgcggggaaa tatcacggat cgggaactcc
    27481 tttggaaatt gcttcaggga gttgattgcg tttatcatct ggcggcaaaa gttatcgtcc
    27541 ccgattcgat tttatatccg agcgagtatg aaaaggtgaa tgtcggagga acggtaacac
    27601 tgttggaagc gatgcgagat gtcggaatcc gtcatatgat tttttcttca tccggaacca
    27661 tttacggatt tcaaaaagag cagcccttaa aagaggaaac aaccaagccc aaacccgctt
    27721 caccctatgc agtatcgaaa ttagcttctg aatactacat taacacgatc ggaagattat
    27781 gggggttgga agctgtttgt ctgcgtattt tcaatgctta cggtccgcga cagggttttt
    27841 catttaccca tgcaccggtt attccgactt ttttaaaaca atctgcggca cgcggaacgg
    27901 ttattgtcca tggggacggt cataataccc gcgactttgt atatgtcgac gatgtcgtta
    27961 cggcattaat tctggcttcc aaaatcagaa atcctcaaga attggtgatc aatattggca
    28021 gcggcatcga gacttcggtc caggaggttc tggatttggc aatcaagatt accggctcaa
    28081 agcccgaaat cgtttataac caacgtcgtc tcggcggacc ggatcgaatg tgtgctgatt
    28141 taacccttgc tcggaaatta ctgaattatg aaccgaaagt ttcactcgaa gaaggtatgc
    28201 gattgactta tgcattggac cggcgattgc ataagcagac cggctaaatt caaagagctt
    28261 tcataaaaac gatcaaaatc tcctccgttt gaaccgacct tttttcgttt aattcaaaaa
    28321 taagaaattt attatctttt tcttcgtcaa gatcgggtag gttataatca aaagatggat
    28381 ttaacagact ggttgctttg tttgggcacg gctgcggcat tcggctttgc attttggatt
    28441 ctgctacccc catacggttg gatagcagga atcgcagtag cgggattgat gttgtatcgg
    28501 gcaaaaaaga agcgagacga tctgaaacag aaataattta tcggagttat taaatggaaa
    28561 ataaaattcc cgctggaaat aataaggttg tcagccttgc ttatacatta tgggttgacg
    28621 gagaaccgat cgattcggct gatgtaaata atcctttggt ttatttacac ggtcataaca
    28681 atattatccc cggtcttgaa aatgaattaa acggattatt ccccggagaa aagaaaactg
    28741 ttattgtatc cccggaagac ggatacggcg aattaatgcc ggattcaatc gttgagttgg
    28801 atcgttcctt attccccgaa gattacatcc cggctctcgg aagcgtttta catttacagg
    28861 atgaatccga ccaagtttac acagcttata ttcaggattt caatgaaaat tcggttatcg
    28921 tggacctcaa tcatccgctg gccgggaaag aattgaaatt tgatatcgaa atagtggaag
    28981 ttcgggatgc ctccgaaggg gaacttaaag caggacacat ccatcatgat tgttcatcct
    29041 gcggcggctg cgaagacggt tcctgcgaat gaaaaattac aaaattccga tggtcccggg
    29101 tccggttcga gtaactcccg ttgcttatcg gtccttgaca tctgatttcg gtgccggata
    29161 ttgtgaagat gaattctttg aactttatca gaccgtcggg aaaaaactgg gcattttgct
    29221 cgggacacaa tcggaagtaa ttattcaatc cggagaggca atgtccattc tctggggtgc
    29281 cattaaaagt tgtgttcttc ccggtgaaaa agtattggca atcagtaccg gatttttcgg
    29341 agaaggtttt gcagacatgg cacgggcgat tcacgctgat gctcaactgg ttgaatttgg
    29401 ttatgatgaa accgtccatg atttggatcg aatcgaatcc gccattaaaa cctatcaacc
    29461 aaaggtcatt acgatcgttc attgtgaaac accttccggt acgcttaacc cgatttctga
    29521 agtgggacaa ttaaagaaga aatacaatgt cccgataatg atcgtggatg cggtagcatc
    29581 agttgcttgt acagaaattg aaggggacaa atggaatgcc gatattgtca tgggcggatc
    29641 tcaaaaaggg ctttctttac ctcccactat cggtttttgt tcggtaagcg agcaagcctg
    29701 ggatattatc cggaaagttg attatacggg atatgaagca ttttggtcct atcacaatat
    29761 tccgcagaaa catgaccacc ccaatacacc ggattggccg ggaattgcgg cattaaatga
    29821 tgtattggat gatctgatga tcaacgaagg tttgtcgaat gtttttgccc gccatcaggt
    29881 tgttgctgaa tttgtcagga aatctttgac agaaatcgga taccgtctgt ttccgaaacc
    29941 ggaagcgatt caatcaccgt ctgttacggc cgtctatgtt cccgccgatc gaacttttga
    30001 agcttttgat caacaggttc gaacctatgg aatgggtatc ggcggaagtt acggaaaaat
    30061 cgccggaaag gtcttccgtt tgggacatat gggaactcag gcaaccatga caaacgcacg
    30121 aagtgctatt gacgtcctgc aacaggtgat ttaatcgatt ctccatgaaa ccgctgaatg
    30181 tcattatcat cggtgccggc ttgggaggtc tggcttgcgg aatcaggctg gctgtcgaag
    30241 gttgcaaagt aaaaattttt gagaaaaacc attttgcggg cggaagcctc ggatcaatcc
    30301 gtttgaaagg atttacgttt aatacggggg cggaatatat ctccgcccct tttttatttg
    30361 atcagctcta tgaatatgcg gggaaacgca gaatcgattc atttaaattt cgggattgcg
    30421 acccgatatt ccaagcggtt tttcctgatt tcaagcgctt tgctctttat tcgaaccctg
    30481 ccgaaaccgt aaaactgaac cctatgttat ccggggcaga cttgcaaggt tttaattctt
    30541 ttacggagga aagcgctgcg attttcgatg atattttttt cgattattgc ggtcgaccag
    30601 ttgatgaaaa cacttttcga tttcgtacga atttatggct gcgtagaatg aacccgcaga
    30661 agacttgtga agaatatgca aatgaaaaat ttcagactcc cgaactgcag caacttttcg
    30721 gtttttggcc attaatcggc ggcggaaatc ccaaaagcgc gacccatctt tttcgggctt
    30781 tttcacaaat gtttcaacgg tggggaacag ccgtcccgga cggcggtatg gataacatgg
    30841 ttcaagcgat tgtacggttg ttccgagaat cgggaggcga gatcttctat gggaccgagg
    30901 tatccgaaat acaaatattc aatcataccg tttccggtgt acgactaaaa gacggcagga
    30961 ttcaacaagc cgatttggta atttccgatg cggatgccgc tgcgacttac cgatatttaa
    31021 tcgattccga tcgaacccgc tatgattcgg tcaatcaggc aaagacaaaa atttccggca
    31081 tcagcgcctt tgtttatcat ctcggaaccc gttgtatcct gcaggaaaga gctcctttgg
    31141 ctcctttaaa tatcttattc ccagaagatt acgaccgatt tttaaatgaa ctttttgacc
    31201 aaaaaaccct ttcggatcat ccattgctga tcctgcgtat ggcttccaaa atcatcccgg
    31261 ataacgcgcc gttcggttgt gaagcgctct ccgtaatcgt tcccgttccc aatttggaga
    31321 gtatcaatcc ctgggatcat atttcgtaca gttatcgaaa tcatgtcttg gatttgcttc
    31381 aaaattattt tctgaccgat attcggacaa atctgatcgt cgaagaatat ctcgatccga
    31441 ttattattaa aaataggttg ggcagccatg ccggatccat tctgtcattt caacccgaat
    31501 tacaaaaaaa cggggaaatc cgaatggcaa atcgttgccg ggatatccgc catctgtatt
    31561 tggccggtaa cggagctcat ccgggagcaa caattcccgg tgtattattg ggagccgcca
    31621 ataccgttca ggtgattaaa gaagatttcg ataaagaata atcgcggatt tttattattg
    31681 aggggaaagt ttactgacaa ccggaatgct tttcttcgta tcataatcag ccagaaagcg
    31741 gtctaaaacg tccaaaaata aagtcatttc ttcttccgta gtctggttca tatgagcgat
    31801 tacaaaataa tcatcccgca tatcatccgg acagttcccg ataataatgc cgtaagagct
    31861 taaaaaatcc gtcagatttt gaaccgtaaa aggaagcggt ttttttaaga ccgtaacggt
    31921 cggtgagcga aattggggac gtgtgaaaat ttcgaaccgg ttggcatctg cccacccctt
    31981 cattttttcg gatagactta cgattcgttt aattcggttt tcaatccctt ccaaaaaaat
    32041 caaatcaagt tgcctgtcca aagcatttaa taattgaaaa ggtatcagag agcgaaaact
    32101 tttagaatgg atatcacgga attgtcttgc atagttcagg ctccatccgg ttcccgaaaa
    32161 accgatggat ttgaaatttg ctcgttcgcc taaaatgacc gtccccaatc ccggcggtaa
    32221 acctaaggat atttcggaac cgattaacaa cgcatccgct tcggacttca gatctaatgc
    32281 gcgaacgctt ccgaaagaag cggaacaatc tacaataacc aaagcatcgg gcagcttctt
    32341 ccgaatcatt cccgataatg cggcaatatc aatttgtgtc cccgtaaacg gatcaatttc
    32401 gaccaaaaaa acagcggcat aggattcctc atcaagtgct ttttcaaaca ggtcttcgtc
    32461 aaagccggaa ccgaaagcgg tatcgataat ctccgaatcg atattcaaag attttgcgat
    32521 tgtctgccat tgctgagaaa agggaccgtt cattccgatc agtaccggtc cgcttgcaaa
    32581 actgtttaaa gcaacttcat acaacccttc ccgtgaacaa ttaaaaaaat taatagaagc
    32641 ggcgtccgga aagaacatct cccgaaaacg gtcttcaatc ttttctgaaa gcgagaaagg
    32701 gttcccgctt tgcggcttcg caagagccgc aagaaccttc ggatcaatcc gtgcgggaac
    32761 cggatttaaa ttaatcggca ttggattatt gtctgtttat aaaaacaccc gtggttgaaa
    32821 agccgaacgg gtctctcata tgattatttg taaacaaaaa ttcgcgggat tcgatcaaat
    32881 aatgaatgac aatatgatca gaatagcgat aaaaacagct gccaatgttc cgattttctt
    32941 tagatttcga attacgtaag tataatccgg attaaattct tctttcggcg cacgagccgc
    33001 taacggcaga ttcccgctgc ttgaaacggc ggtagcagtt gttttaatcg gtgccttccc
    33061 ttttgtcgta tttttctttg ccattaatcc ctcccaaagc atttattctt ctatagcatt
    33121 ttccccgtga caactatcct tttatcatct accaaataag gatagcaaga aatcaaagtt
    33181 acagtcggat ccgttgtctg tgccataacc tccacctgat tcggcagaac gacttctatg
    33241 ttttggacgc tataagtata agcgtttttt tcggttaata cgatgatttg atccccgggc
    33301 attaattgat ccaaatcgcg aaaaacttgg ccataaatat cattatgacc ggaaagaata
    33361 atatttccgt ttttccccgg agccgcactg tacagattca ttccgatccc cgctttcaac
    33421 gcttcccaat catcccccaa aacgaccggt gcatcgacat taatcgccgg aattctgagg
    33481 cgaatgacct gttccatacc ggcgggaatg gtcggttgag gagccattac cgaaacaata
    33541 ccgcgcaaat gctcgggaat ttcgctttca ttaaaggtac cgacaccgct ttcatccgga
    33601 ggagtatgac cgccgggcaa gacaacagcc tgaataagcg gagtgggtgt cagagtgggg
    33661 agaatcatag attttgccga ttcccgattg atgttttgca aaatttgaga accgataacc
    33721 agcataacaa tgacaccggc caccgccagc agctcgattg ccaataatat tttgtcgaat
    33781 tttttctgag gcggtgcggt tttgagaact tccttttcgg atctgtcgga attccatttc
    33841 aattcggaat tgatcggcgc attcgctgcg tccatcacgc gaccgccggc gcgataggaa
    33901 gcgatacgag catctcgtcc gatggttttt ttttgcatca aacggaatcg caattcttct
    33961 tcggttaatt catccgcata tttttttcgt ttcatcactt ttaattatat tgcgtctatt
    34021 cgtcgatgat ttgatttaaa gttgtcgata ttaagaaaga atataaaatc aacaaagatt
    34081 cttgcaaaaa aattaattaa aaataaaagt ctttgaaaca tagtaattaa agaaaaacat
    34141 ccccaactcc gccgccaatt tcaaaaatat gggatgaata gggattattt tttctccgat
    34201 agtgatcaag ctccaaacaa cggagcctga aaaaatcacc aagccgacat attttgcaaa
    34261 agagcggttc ttttcatttt tcggcttaaa aacgacggat ttcagcaaga aataatttac
    34321 cagaagcgaa gctacccggc cggtgaccaa agccaaaaga ttgccgaatc cgatttgtat
    34381 acccagaaaa aaaacagtat tgtctacgag gaatgaaagg acagaacttc ccgaataacg
    34441 caaaaacgta ttcaatgtat aaaatagcag gttgattctg cggaggctgt ttaccgcaac
    34501 agccccgtca tttttccgat ttactcgtgt cagaaaaatc gggatgcctt gtcgctcaca
    34561 tttcaacagc cacgggaaca aggaaaactt tttatcttcg gaatatggat ttgccgaggt
    34621 gatcgcctgt ggtgggataa tccataagga agccttcgga ccttccaact tccttcgaat
    34681 ccaccgaatc attcggacga tcccattatc ttcaacggat tcctgggtcg ttaagatttc
    34741 caatagataa aaggcttgcg gattttttcg ggccgatgcc aattgttcat tcaattccga
    34801 atctgaaata accgcatcgt ccgaacaaat gacaaaaaag ctttcgatcc cggtattttt
    34861 ttccgagtca aataataaat ttccaagagc ttcctgaaag ttatttcctg agaaaggaac
    34921 gacgggaaca gatcggataa attcattttc ggttcgtacc ggttctgccg acaaaaaagc
    34981 aactggtctt ccgccgagtt tgaccaatga attaaaaaga gaaataaaat cacagctttg
    35041 attcaaatga ataaaaataa aggggaatga ctgttcgggc acgattttcc ttacgcttca
    35101 ttattttcga cacatgcatt ttaacggatg attctcgtat ctgccaatgg aactgataaa
    35161 cgcagagtaa aataaacctt aataaagtat gggattaaaa aaacggtagg gagatattta
    35221 tgaaaatagc aattatcgga tcgggtctga ccggcatgac cgcagcctgg aatctcagaa
    35281 aacagggaca cgaagtgacc atttttgaaa aagaaaagga acccggcgga ttagcccgcg
    35341 gatttcgtga acctcaatgg caggattccg ttgaatgctt ttatcatcat attttcaccg
    35401 gtgattcgga tattctttct ttatttaaag atctcggggt atcaaattcg gtagaattca
    35461 gatctcctaa aacagtcatg tattatcggg gaaagtttta tccctttgat tccataccgt
    35521 ctgctatcct ttaccccggc ctcggcttag gaagcaaaat ccgttttggt tttgtcggtt
    35581 tgtatttgcg acttttcagt ccatggaaag aaatggaaaa agttaccgcg gaagaatgga
    35641 tgctcaaata tgccggagaa catgtttacc ggtccatgtg ggaaccgatg atgcttggga
    35701 aattcggtga gaaatatgca aaagaagtca acatggcatg gctttgggcc cgtatccatt
    35761 ccagatccac atctctggga acctttcgtg gcggctttca gactttcatc aatatttttt
    35821 ccgaaaagtt gattgcgaac ggtgtggaga ttcgttttga atcgaatgtc acacagataa
    35881 agagacaaaa agaatcttcc ataacagtaa ctgcagacgg aaaaaatgac gattttgatc
    35941 gggttattgc tacgatatcg ccggcggctt tttcggctat atcaccggat ttacccgccg
    36001 attatcgtca gtctttactg gatctgaaga atatgggcgc ggttgtaatg gttttatcgt
    36061 tgaaacatcc tctctccccc gaaggttatt attggtataa cctccccaag agtgaagggt
    36121 ttccttttct ttctttggtg gagcacacca actttgttcc gcgagaacgt ttcggaaacg
    36181 aaaccatcat atatgcggga gattatctgg agaccgatca tgaatatttc tctcttccga
    36241 aaaatgagtt gttgcaacgg gttttacccg gacttaagaa aattaacccg aattttgaag
    36301 aggattgggt tataaacagt tggaaattct cggcaaaata tgctcaaccg attcctttta
    36361 tcaatcaatc cgaacacatt ccgtccatag agactccgat taacgggtta tatttagcat
    36421 ccatgagcca aatttaccct tgggatcggg gaatgaatta tgcggtaagg atgggaaata
    36481 ctgtagcccg gatgatcgga taatgatttt tgctaattcg aatttctggc aattatttgt
    36541 ttcaattcac tttgtaattc ttaaaatttt tttgatttga atagacgttc taatatttat
    36601 gcgaattaat cctcagctga tttcttgtct gaatatgaga aaattttgct gatgatatcc
    36661 aaagtaaaga agcgaatcga tcgaaacacg cataatttcg gaatgcgatg aagtttcttt
    36721 aaggttgatc aaccttaaag aattcgattt ttttaagttg tttttcggta atattaagaa
    36781 gaccttaatg ttagaataaa ttcagttcga taaacaagca aacatttcga accaagctca
    36841 ataataagtt ctttggaact taatcataaa tattttttat acaatttcgg ccacaagaca
    36901 agtaacattg tggttttatt gtctctatat ggtaaaaaaa tggctcaaac tgacgaaaca
    36961 aagataacta cttataaaat aacccccgaa aaagcgtggg aagcagccat cggccaactg
    37021 aagataacga tggataagca aaccttttcg actttaatgg gaaaatcgga atttgttaag
    37081 tatgaaaaca atactttttt tatcagcgtt ccgaatacct ttacaagaga ttggttagaa
    37141 caacgattaa cagaaacggt gattcgaatt ctttccggaa taatggaggg gcagcaattt
    37201 gtccaatttg tattggaatc gaatgttaat gccattcggg aagaaaaaac ggttttatcg
    37261 caacccgctg tcgcgacagt ttccgcatct caatccgtct gtcgggaagg ggctatcaat
    37321 ccccgatata cttttgataa ttttattgtc ggtccgtcca acaatcttgc ctatgcggct
    37381 tcacgggctg tcgcggcaat gccggcgacg gcttacaacc cgttatttat ttacagcgga
    37441 gtcggattgg ggaaaacgca tttgctccat gcgatcggaa atgaaatcat tcggaatcaa
    37501 aataaagcgg tgttatatgt ctcatccgaa gaattcacca acgattttat caactcgatt
    37561 caacgacatg agaatacggc tttccgagag aaatatcgca acattgatgt attgctgatt
    37621 gatgatattc aattcatcat cggaaaagaa agcacacagg aagctttctt ccataccttc
    37681 aatacgttgt atcagcagga taaacagatc attatcactt ccgatcgttc tccgaaatcc
    37741 atgtcgacat tggatgaacg gatgagatcg cgttttgaat ggggcttgac ggtcgatatg
    37801 caaactccgg atttggaaac ccgaatcgca attttatctg taagagccga aaaagtaggc
    37861 aaaagcatac ccagagatat tcttgaaatg attgccaggc aatttccgaa caatgttcgt
    37921 gaactggaag gagctttgaa tcgggtttta gctgtttcag atttgtgcgg aaaaactttg
    37981 gataaggatt tagtcttaat gtcattgggg gaattgttgc cccaacaaaa tgccacaccg
    38041 cttgaacaga ttctgaatgt cgtttcaaag gttttcggag taccgaccga aaaaatatta
    38101 agtagagacc gatgtaaaaa tgtcgcttta tctcgacaag tggtcatgtt cctcttacgg
    38161 aatgaagaaa atctttcgtt accccaaatc ggcaaggaat tgggaggaag ggatcattcc
    38221 actgtgattc acggttgcga gcgtgttgct tctttgctgt caagtgacgt tcggtttcgc
    38281 aatcttttcg aaaaagttca tgaacagctt tacggtgagc catgttttgt tatggcgtaa
    38341 tctaagattt ttccatttta ttcgtcgggc tcttttttaa aaagagtcct ttttttattc
    38401 cgatccggtt ttggaacatt tgggtcgatt aagacgttct tagaatagct ctttcaggca
    38461 gattaatcag caagaaagac gagtaaatca aataaaagga aatcagtata aaacttgcac
    38521 cttaatccgt attcagttcg gattgttgaa ttggaaatat ttcgaaaagg aggtttgcgt
    38581 gtcaaaaaga aatctgatta tcattttcgc cgttgttgtg atttcgcttg caggctttgg
    38641 ggcggcatat gccagcggat caacccaata ttcatggcca aacaattgcg gcaattggaa
    38701 ccagtgtaac tgcggatgga ataactgttg ttgcaactat tatgtccccc cgcaaccgtc
    38761 attatgtgca tctttcattg ccgatgtcac cgtcccggat ggatcttata tcgctccggg
    38821 aagctcattt acaaaaacgt ggcggattcg aaacaacggt acgaccactt ggaataccaa
    38881 ctataagtta ttcttctcaa gcgggagcca aatgtccgga ccgtcatggg tcgcaatgcc
    38941 atataatgtc gctccgggcc aaacggtgga tctgtcggta aatcttatcg ctcccaccac
    39001 aggcgggact tatcgcggca attggaagtt gcagtcggat acgggcgtca ccttcggtgt
    39061 cggatccact tgtcaggttc cgatctgggt cgaaataaag acccaacaac cggtctatta
    39121 caacaactgt tgttacggga attgctgttg cgtttatccg tgtaattgcc catgcactcc
    39181 agcggttcct tccactccgg tgaattggca gaattggagt acaactcaag ccccggcatg
    39241 gccgacttgg cctgattgga actgaggatt gtttcgttaa aatcttcggc ggaaaatcaa
    39301 caataaaagc agagcctttc atttaaggat ggctttgctt ttattttata ttcctcgaaa
    39361 atccgaaaaa gcaaagttag cacgaatctt gcaccttatt gaaaacaaat tgattgattc
    39421 tctccggttg gaacgggttc aaagctttca tggaaaaata cagggaaccg gagaatataa
    39481 gctgattttg agaattttca atagggacca agaggaggaa attgtgtata aaaagcaatt
    39541 ttttacaatc atagcaatgg caatattcac cgttttgatt ggagtgattg cggttcaggc
    39601 atctcccgac gttcagggtt attacggatt taataatcct ctttggggaa atatggatca
    39661 accgtggaac tataactata acaacagtta taactatact tataacaatg cctatagtta
    39721 cagtcctcaa gtctgcaatc aatgcacata tgcttgcaat cgggccgaat ttgtaaagga
    39781 tgtgacggtt ccggatggaa gttatatcgc tcccggcgct tcctttacca aaacctggcg
    39841 tattcgaaat accgggacca ccacctggac cacagcttac cgtctcgtat tttccggagg
    39901 agattcctta ggcgcaccct catcggttgc ttttccgtat tccgtatccc ccggttactg
    39961 gatggatatt tccgtacctc tgagggcccc caatgcagcc gggacatttc aaggaaactg
    40021 gatgctccaa agcagtgacg gcacattatt cggtgtaggc tgtaacggtc aggttccgat
    40081 ttgggtgaag atatcaacgg tcagcaacag ctgcgccact gtcgcctatt cttgcaacaa
    40141 ttgcaatgta aactactgta atccggcctg ctatggatcc tactataacc agatcacaaa
    40201 aaatccttat tgcaacaaca aaattcgctc cgtaaatgac atcacgattc cggacgatac
    40261 ggaaatggat cccggaactt ctttccgaaa gacctggagc atgaaaaacg gcggcacttg
    40321 tgtttggaat gaaggatatt ccctgatatt cagcggcgga gatgcgatgg gctggaaaac
    40381 tcccgtcttt ttaccgaatc ccgtttatcc gggcgaaacc gtcgaaatca gtattgatct
    40441 aaaagccccc aacattccgg gagactatcg tggatactat aaattgcgcg ataatctggg
    40501 gtttactttc ggatacggat cctatgccaa taaagcgttc tgggtggaca taaaagtcac
    40561 cggcagtccg cttccgccaa caaaaactcc gacaccgacg ccgaggccga cagcgacacc
    40621 aaccccgact tcaactccgg ccgctagaac agcagccgga tcgggacagg aattatcctt
    40681 agtcattcaa tccgaagatg atgattcatt ggttgaacct ttggaagatc ctcttctttt
    40741 agatgacgat gaatccgaag cggatttatc gaacgtactc gaagaaggag aaggagtaaa
    40801 tcagaaatcc atcgcgtaca atgcctgtgg caagcagcga atcgagatca atttcaacaa
    40861 gccggatatc tacaatatta cttggtatgt caccaacgcg gggtctgcgg tttggaactc
    40921 ggataaatat cgggtagtga attcgggttt gagcgataac ctcgttatga tggggacgga
    40981 tatcaccgtc ccggcaacaa atccaggaga agaggcaatg gtatcgatgt cggttaaagt
    41041 aaaagatgta gctgaccaat cggatcgatg gctaaaatat tacctgacag acggaaacga
    41101 atcattctgc gaggtctatt tctcccttcc tattttctaa aacccagaaa ctgcaaaatc
    41161 cccgtcagaa cccctttcgg ggtttttgca gttaaaagag attttttatc aatccatttc
    41221 tttgtggaaa caatcagaat aaatcgaatt ttggaaatta tttatagaat attcgaagac
    41281 aaaattttct tcatttgtca gttttccagg gataagctaa aattggattt gcc
//
LOCUS       DAOSMM010000013        40596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1026_length_40596_cov_15.978417, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000013 DAOSMM010000000
VERSION     DAOSMM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 40596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40596
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1026_length_40596_cov_15.978417"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK898_03555"
     CDS             <1..543
                     /locus_tag="PK898_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19142.1"
                     /translation="GEELQIPVFLYGEAAIIPFHRKLSNLRRGGFEKLRELVGTTPDY
                     QPDFGPAYLGPAGACAVGARFFLIAFNIYLSTSDVGVAKKIAREIRESSGGLPKVQAL
                     GMLVNNLAQVSMNLTDYRITPIDTVYEAVEKSAYQKGITIDHSELVGCIPKAAIQGID
                     PDSIKLKDFSPDRILENHLP"
     gene            785..1741
                     /locus_tag="PK898_03560"
     CDS             785..1741
                     /locus_tag="PK898_03560"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOU19143.1"
                     /translation="MTEQRLPSYGGQALIEGVLMRGANHVVASMRAPDGHIEVVHEEL
                     GGIYKSRIRNIPGLRGLISLWDSLGLGLRFLTISANIQTGEDEKIEGPEMVITMIISI
                     VIAVLLFFLAPVWIAHWIESMTNLTQLGANIIEGFLRLAIVLGYMVIVSRMEEIKRVF
                     RYHGAEHKTINCFDNGLDVTLENVRTQSRLHPRCGTAFMVTVVLISIILFIVIGPFTK
                     TLTSRLISRVLLIPVISGIAYEYIRWSARHLNNPLVKILIYPNLMVQKITTADPDDSM
                     LECAISSFLTMLAYENGTMPVEVNTPIKTAEYATIGSEKTIP"
     gene            1760..2317
                     /locus_tag="PK898_03565"
     CDS             1760..2317
                     /locus_tag="PK898_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU19144.1"
                     /translation="MNKWQILVSGILIGFLFSALIILFADQQKGKPILLQAESTPGTI
                     QFEVLGAVRNPGIYSSESIIRVIDAVNLAGGLTENADAQNSNLTEKIYDTDRIIIPTI
                     SLVQATTTSIATINNQKRINLNTASLDELMLLPGIGEKKAKDIIEYRTNIQPFSSVSD
                     LLNINGFGEKTIEEFYDQVTVESGG"
     gene            2320..2769
                     /locus_tag="PK898_03570"
     CDS             2320..2769
                     /locus_tag="PK898_03570"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOU19145.1"
                     /translation="MGIKNDLENKLKDAMREQNEDKKRVYRMALSSIRFEEKTKNKEL
                     DEESVQAIIQKEIKIRNEMIEDAMKAGRDETVNEKKAEISLLEEFLPKQLNDEELSRL
                     ISETVQEVQATGPSDMGKVMKTLLPKVKGLCSNEKVSKAVKDYLANL"
     gene            2766..4946
                     /locus_tag="PK898_03575"
     CDS             2766..4946
                     /locus_tag="PK898_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU19146.1"
                     /translation="MTENGGAESRFSKDTAYLRGVIFCITVLITFALLTFQISVRKTS
                     YSLSVGDVVNQDIVAPRTITFESQILTEQARNDAQNMVDKIYLPADPTISRNQVQYLR
                     LSLQFISAIRSDQYATEAQKIEDIQKLIFVRLDEPTIKQILELDDSEWKLVQSECQRI
                     LEQVMQNSIRDDQVNSVIENIPTMISYSINPKTADLINSITRRFVVPNSLYSAELTEK
                     KRQEARDAVEPRERTFIANQTVVSRGQIITELIYEALDKMGLVHSKNDNRKILSSGLL
                     VLGLTVFALYFFLIDHRHHQLNLRECILIAGLYLAFLFAARFLIPNRTILPFIFPIQT
                     LGLTISCLSGQAFGIVMSIIIGLLVPYDFPNALGYASFYIISSLTGILVLRKARQVTD
                     FLLAGLAAGSIGIPIIIAFQVAESPMDMIGVVTLAAASVGSGLLSTGFTLIFQYSFSK
                     FIGVASSLELLELIRPDSPLLQYILNTAPGTYQHSLMVANLSEQAAKEIDADPLLVRA
                     GSMYHDAGKALNPSFFIENQVNGSINLHEDISPKESAASIIQHVNDGVMLVEKYRLPE
                     VIKDFVREHHGTNVARYQYTEAVNQEGEEAIDINDFTYPGPKPQTKETALVMLADSCE
                     ARARGDRPKNDEEIEALVKSVFDYYSSSGQLDDAPLTLRDLKKIRESFVRVLRNSYHP
                     RVKYPEQKKESKNKTASVVKNNRENRTGKTAEFESAEANIQRDK"
     gene            4952..5422
                     /gene="ybeY"
                     /locus_tag="PK898_03580"
     CDS             4952..5422
                     /gene="ybeY"
                     /locus_tag="PK898_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOU19147.1"
                     /translation="MFIDNRTKKADLAIDSFKLDQIAESVLSDFLEQPAQCTLSFVKP
                     SEIRKLNKQYRGVDEITDVLTFQSEGEIDPETNIPYLGDILICIERASEQAEISGHSL
                     ENEVELLLIHGLLHLIGFDHDTPANKDLMWEKQTDYLNKFNIHLGRKPGDDFEF"
     gene            5412..5786
                     /locus_tag="PK898_03585"
     CDS             5412..5786
                     /locus_tag="PK898_03585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOU19148.1"
                     /translation="MSSKFIRNRNVSFRNAFNGIIHGFVDEINFKIHLFFTILAVLLS
                     VILKISLTEWCLIIIVIGMVWSTELINTAIERAVDLYTLEKHPLAKQSKDIAAGAVLI
                     SAITSVMIGVIIFLPKILIQFF"
     gene            5949..6236
                     /gene="rpsF"
                     /locus_tag="PK898_03590"
     CDS             5949..6236
                     /gene="rpsF"
                     /locus_tag="PK898_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOU19149.1"
                     /translation="MRKYEIVIIANPDLDEESINGIVEKVSGWIKDSGGEIEKVDDWG
                     KRRLAYRIQKQREGHYVLITANIEPSAMKEISNNLRFVESVLRSMITLVEE"
     gene            6309..6566
                     /gene="rpsR"
                     /locus_tag="PK898_03595"
     CDS             6309..6566
                     /gene="rpsR"
                     /locus_tag="PK898_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOU19150.1"
                     /translation="MDHRGNRRFIARPKFCQFCSEKNTKIDYKNADLLRRYITEEGKI
                     RPRRQTGTCAKHQRQIAAAVKRARYIALLPYTSEPYQDVVR"
     gene            6642..8000
                     /locus_tag="PK898_03600"
     CDS             6642..8000
                     /locus_tag="PK898_03600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU19151.1"
                     /translation="MPNQEEKKENTHPLLLNKERKSRHEKEMKQMKWLKIGGITVFAI
                     VILVLVFGAFYEYQYLPNKTIAKVQDKKITVKEFQEAVRYQRVNLISNYNYMAQLYQS
                     FGMQMDEETKSQYETQLSEPYAGILGQQVYNTMVNQLILEYGAKAAGITVSDAEVDNE
                     IQSMWGYYPNGTPTPRPSETPFANTPTVSEEQLKLLNYTETPTPTTEITDLTSLKESG
                     PEANTPVVTDTPTLSPTATQTEVSSESPTPVLSPTPSLTPTTYTEEMFQNNFNRQFEN
                     NPYYSKEFFRKQVYYQLLEQKVRDMLAKDIEPEAEMVWARHILVSTEDEAKKVIERLN
                     NGEDWNALAAEVSTDTSNNTKGGDLGWFMKGVMDPDFEKAAFEQEVGTYSKEPVKTQY
                     GYHVIQVVGHEVRPLTSTDRSNAIDRAYKNWLENAKSNLKIETGSDWVDFVPVEPTLS
                     DL"
     gene            complement(8090..10570)
                     /gene="lon"
                     /locus_tag="PK898_03605"
     CDS             complement(8090..10570)
                     /gene="lon"
                     /locus_tag="PK898_03605"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOU19152.1"
                     /translation="MSSSNWLEDKDLVNDFTTNINQRTEELFRIPDMELDSDGCFVAP
                     VVVLRENVIFPKMITPVFVGQQKNLESLRYGLENEQTVITLIPKDPEKEFPVKKSDFY
                     PVGLEIAVGRLVNLPEEHFSTLVQGRRRVILMDIVRRSPVLMARVYVSEEEAKKSRHL
                     EALIRTTRNLFEKCVQLDRKLPDEAVAYASTITDPSWLADMIATTISFENEQRQEILF
                     ELDPEKRLEILNSFLAQELDVLELGNQISTTVQNEVDKNQREFYLREQMRAIQKELNE
                     DDVFSRDLNELKEKIDQKQFSDEAREVALKEFERLTQLPAMSPEVNVSRSYIDWLLDL
                     PWVEQTEDNLDVKHASEILNKNHYGLEKAKDRILEYIAVRSLKPKKEKQPILCFVGPP
                     GTGKTSIGRSIAEALGKKFVRISLGGVRDEAEIRGHRRTYIGALPGRILQTMKKAGTI
                     NPLFMLDEIDKLGNDYRGDPSAALLEVLDPEQNSRFSDHYLEVDYDLSKVMFITTANS
                     IEMIPDALLDRMELIEFPGYIEEEKLIIAKDYLINRQTEENGLEPQDINFTDDAIKRI
                     IREYTYEAGVRNLEREIGKICRKIARLKAEGKEYEREISAESVGKYMGPPYFFQSEKE
                     KEDEVGVATGMAWTPNGGDITTIEVLVFDGKGNLQVTGQIGDVMQESAQAALSFVKSR
                     AKQFNIPKDYFEKTDVHIHIPEGAVPKDGPSAGVTLAIALISAITNTPVKKDIAMTGE
                     VTLRGNVLPVGGIKEKVLAAHRLGIKTIIIPEKNEKDLEELPQQIKEELTITSVNHMD
                     QIIPLAFGNIQPKRKTEVRAKKDKKDES"
     gene            complement(10585..11982)
                     /locus_tag="PK898_03610"
     CDS             complement(10585..11982)
                     /locus_tag="PK898_03610"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOU19153.1"
                     /translation="MLAHLETIDDALDAIYSFVDYSMTHAEEISSAVFSLNRITELLN
                     RLNNPQEAYRCIHIAGTKGKGSVCTMIYEGLRASGYKTGLYTSPHLIHFNERIQVDGK
                     MISDAELIQLTEKVIEKIDPANPPSSFDMMTAIAFEYFREQKVEYAVVETGLGGRLDS
                     TNVVHPVLTVITSISMDHMNFLGDTIEKIATEKGGIIKQNVPLIAAAQDPKAMEVIRK
                     IAEERNAEWIDVDKNYGFVPLKDSVDGQRLMIWERKEQNALRNWLENREKSIWKPWEV
                     DIPLAGYHQSINAVIAFTALMKIRSFADKINEKSISDGLAKTFWPCRFEKVSIDPPVI
                     LDGAHNPDSIEKLVMTLNRFYGTKRITCVFGCSEDKDLSGMIRILAPFVSDFIVTRST
                     HPRSMETKQIYDEVLSNGRPAVIRESLELALRDIDSGDKNNVYIVTGSLFVTAGIREL
                     LMKRNPDMKYFEKKK"
     gene            complement(12036..13661)
                     /gene="treS"
                     /locus_tag="PK898_03615"
     CDS             complement(12036..13661)
                     /gene="treS"
                     /locus_tag="PK898_03615"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HOU19154.1"
                     /translation="MNINPLWYKNAIFYQIYPRSFYDSNGDGYGDFQGIIQKLDYVRN
                     LGTDCIWLMPTYKSPLKDDGYDISDFYGVFEPFGTLDDLKQLFNEIHQRGMRVITDLV
                     LNHCSDQHPWFQSARKDPNSKYRNYFVWSDTDTKYQGTRIIFVDSQKSNWTWDEEAGA
                     YYWHRFYPSQPDLNYDNPEVREEMKNVMRYWLNLGIDGFRADAVPYLFEREGTINENL
                     PETHAYLKEIRKMMDLEFPGTILLCEANQWPRDVREYFGDGDEFQLGFHFPLMPRIFM
                     SIGKQDASSLVQIMQETPEIPENCQWSTFLRNHDELTLEMVSEEDRQWMWAFYAPQKE
                     MRLNLGIRRRLYPLLDNDRKKIGLAFSLIFTLPGSPVLYYGDEIGMGDNINLFDRNGV
                     RTPMQWDDTKNGGFSTADRTYLPAITDEEYGYQKVNVMQQEDDPDSNLNWLRRLIRIR
                     KASEALGEGELEWITTGSEVKVLAYIRRSENDLVYVIHNMSDYPMHFQLDFEPGIYTD
                     LIQPDFTIAGNRDTELDLNPRIFYWLQRKKSIG"
     gene            complement(13770..14897)
                     /locus_tag="PK898_03620"
     CDS             complement(13770..14897)
                     /locus_tag="PK898_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU19155.1"
                     /translation="MAQKEKSYKSQKKTSSFIVNLVLILISAVFVVPIFGILITSFRQ
                     SERIFNSGWWTVFPHKEWVEIGEYKLDADIDLNGEIKFVSEDGQEISGDYSFWKNGVI
                     TKSGEKIQYYGAKRSRTLKIYERRWTGFETDLTLENYRNVLSGKKITYKGEDGKLLTR
                     NGSDLSNSFINSLTVTIPATIIPITIAIFAAYGFAWLNFPGRKILFTMVVALLVVPLQ
                     IAIVPVLQDFSRIHLNGTFLGIWLAHTGFGLPLAIYLLFNYISEIPRDILESAFIDGA
                     TNFSIFTKLILPLSVPAIASFAIFQFLWVWNDYLVALVVLGGGTNEVVTQTLANMVGT
                     KGQDWHLLTAGAFISMIIPMTVFFSLQKYFVRGMMAGSVKG"
     gene            complement(14897..15991)
                     /locus_tag="PK898_03625"
     CDS             complement(14897..15991)
                     /locus_tag="PK898_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU19156.1"
                     /translation="MNKTQTFGKSPSKIVLFFKMILILAGIILIFTAGVRIITFLDTN
                     HLKIPSALFAIVWGVSTVVLFYTGANWIVEHFSPKWTARIQPYIFVGPAVIILIWALT
                     LPVFRTLISSLKDADAKNWVGLQNYKYAFTDDQMLIVFRNNLLWLILGTFFSVFLGLL
                     IAVLADRSKYEKVYKSIIFTPMAISFIGAGVIWKFIYNYKGQGVETGLLNAIVMSFGG
                     QPHAWLQIPFWNNILLIVIMIWLETGYCMVILSSAIKGIPEELLEAARIDGASETTIF
                     FKIIIPVIKGSLIAVTTTVAIFSLKTFDVVQSMTGGNYGTNVIANEFYNQRFLYYNIG
                     RASAIAIVLLILVVPVMIMNLRQFNERKVF"
     gene            complement(16127..17443)
                     /locus_tag="PK898_03630"
     CDS             complement(16127..17443)
                     /locus_tag="PK898_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19157.1"
                     /translation="MKKVFSLILAVTMLFGAAAMVSAQDKTYLERAYDGEFKGKIVTL
                     DGPFIDNDQVLFEASIKDFEEKTGIDIQYIGNKDFESTIQMRVEGGNAPDIADFPQPG
                     GMANLAKKGYIVDVRDFISEDWLKENYSQAWLDLATVPNEKGEMIMGGIWARNNAKSQ
                     VYYRPKVWEEAGYETPETWQEMVDLSKQMVADGETPWCLGIEGGAGTGWPATDWVENI
                     MLRTTSLENYKKWTTGELKFDSPEVRRALEIMSEIFFTDGFVYGGREAIATTNFGEAV
                     KPLFYDTNGCMMYSLGNFITAYFPEDAVPGVDYDVFYLPPIDEQYGRPFLGAGDVYSV
                     FKGHDRDEVKATLEFFTHGESLKVWLANGGAMFPGKDADPSWYANATDAKISQMINDA
                     TSFSFDGSDQMPAAVGSGTFWKGMVDLVTGTDIDTVVKNIDKGWEQ"
     gene            complement(17494..18489)
                     /locus_tag="PK898_03635"
     CDS             complement(17494..18489)
                     /locus_tag="PK898_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU19158.1"
                     /translation="MGKTPTIYDVSELAGVSTATISRVLNNPEKVKPCTKEKVMMAIQ
                     ELGYRPLLEARLRTAHDFERICVCAPRFTENSFVERLRGITVALAGQDYELQVYSVAS
                     KLQMDRFIDSLELRGLNGLITLSIPFTDEQIKRIKECGIEMVMIEYSSPLTNCVVIDD
                     RMGGKLAAQHLIEKGRKKFGFAAELNKSDYSVYPISGRLEGYQSTLNSHGFELNPRYI
                     YDVKCDTDQTYLNFLKIFRSGEFPDAIFAAADVQALGILRAAKEVGLNIPQDVAVIGF
                     DDIDFADYVGLTTIRQNLDHSGKVAVDLLINRLKDPERLIQLVQLPVELVVRKTT"
     gene            18695..20593
                     /locus_tag="PK898_03640"
     CDS             18695..20593
                     /locus_tag="PK898_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOU19159.1"
                     /translation="MKPQEIEKEIEKFLLKVQKPGRYVGGEFNSIYKDSSAIQTRIAL
                     AFPDIYDLGVPNLGIAIFYDLLNKLPNVWAERVYLPWEDMEKEMRERNIPLYTLESKT
                     PVNQMDILGITIPYESLYTNVLNLLDLSGLSVFAAERSEEDPLVIAGGHSTYNPEPMS
                     AFIDAFVIGEGEEVFPEIVTVYQLWKKQALPKSELLYRLSKVEGVYVPLFYQTTYNGD
                     GTVSAINPVNDSFPKRIKKRILPILPPPPDYFVVPSIDVVQNRIAIEIMRGCSRGCRF
                     CQAGFVTRPVRERPAEQILESIDSALSSTGYEEIALLSLSSSDYTQIQTLVDALQNRY
                     GKRKLNISLPSLRIDTVSVNILEKLKGSRFSGFTLAPEAASEKMKKTINKPITEEQLL
                     ETAEMIYSHGWQTIKLYFMIGLPGETDEDVLAICDLCLKVIRIGRKYLGKRSQLNIGI
                     ATLVPKPHTPFQWWKADEPENIARKQDLLKKGLSTPGIKITWSDPDTTMFEAWCSRGD
                     RRMGAVIHRAWQLGAKFDAWQDHFNKEAWLQAFSENGLDPDFYVHRERSKNEVFPWDH
                     IDIGVTKHTLRKEFENGLEGVLRPDCRTGCYGCGINQAFRLVQPKAGEQKWFCPILNF
                     SAERELTE"
     gene            20590..21249
                     /locus_tag="PK898_03645"
     CDS             20590..21249
                     /locus_tag="PK898_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOU19160.1"
                     /translation="MIRCRISYQKTGPLRYISHLDLQKVWIRVLLRAKIPLIYTQGFH
                     PAPKIATAWPLPLGWEGKNELIDLWFDWEDDFDTEDQKKRFIETLNSQSPLGLSINSA
                     EEVPAYSPALTKLIESADYRIKTAGRFLAEDLNNKIHHLLSQTSVERIRREKRYNLRD
                     LIESASVSEETDEGPVIHVRMIAKDSAMGRPDEFADALGLDSEYLSFCRDRFYLSIPE
                     H"
     gene            21281..21352
                     /locus_tag="PK898_03650"
     tRNA            21281..21352
                     /locus_tag="PK898_03650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21314..21316,aa:Arg,seq:ccg)
     gene            21363..21447
                     /locus_tag="PK898_03655"
     tRNA            21363..21447
                     /locus_tag="PK898_03655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21397..21399,aa:Leu,seq:tag)
     gene            21599..22849
                     /locus_tag="PK898_03660"
     CDS             21599..22849
                     /locus_tag="PK898_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU19161.1"
                     /translation="MEKNTTVNKKSSRFTPVVTVIIIFGIISLLGDMVYEAARGANSQ
                     YFNLLNINAAQVGLVFGIGEFLGYMLRLFSGIISDKSGKYWLFIFIGYGMLIVIPLMG
                     FTKNWNILVVLILLERIGKSLRSPAKDTIVSGVAENQVGIGFAFGLQEALDQIGAFLG
                     PLIFTLVFYLAGKDGISVYQLGYKVLVIPFILLMFFVTFAYRKIEREHLVPSLDAKEF
                     QNEKLGPTFWAYTAFTFFCTLGFVNFSTVGFHLKANNLMSDGNITLLYAIAMIVDALT
                     ALIVGRAYDSQKKKTGRKTGGLLVLALIPFITFLLPFLTLSYSTTLIIAGMVIFGIVM
                     GTHETIMRSAIADIAPFHKRGTSYGVFNTGYGLALLGGSSLMGWFYNLDKIGLIIGLT
                     CLSEFIAILFYVKMNHLVKASAKQ"
     gene            23055..24365
                     /locus_tag="PK898_03665"
     CDS             23055..24365
                     /locus_tag="PK898_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19162.1"
                     /translation="MTSSQIVEIQQNRFSRQLRHVLRYSEFYRNFYRKAGIDIERPEL
                     IQLKDLPVIDKDLMMDHFDEFVCNKQLKKSKLEDFIKDPNSVDKKYKDEFKVIHTSGS
                     SGRIGIFVYGANDWAIAEAAVYSRISKNKLHFIGKAKHAFIGVTDGHYAGISLAKSGP
                     KWLFDFLPVDMNQPLHPSLVRLNDFQPECLSGYSSGVYLLALEQLKGNLKIHPTRILC
                     SADPLTSEMRETIRNAFGIMPYNYYAASESLCMGIQCDRFNGIHLFDDLHIFEIIKEN
                     GEEAKPGEPGNLVMTNLYNKTQPLIRYRMNDNLIPSENSCECGWPFRSVESIAGREEE
                     FLFFTDPAGNRQFIHPIVFVEFFAPGLEKFQIIQTEPNMLILNAIIKGNKEEAIQAIE
                     KRMDTILHQKELYDFVHYKINVVNDIPNDPQTGKFRLIIPYPNS"
     gene            24575..26077
                     /gene="tig"
                     /locus_tag="PK898_03670"
     CDS             24575..26077
                     /gene="tig"
                     /locus_tag="PK898_03670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOU19163.1"
                     /translation="MKIEKQPLENHEIKLEVEISPEEFEPLKHQAAKKIAKDAKIPGF
                     RPGKAPYEIIRRNYGDTAIAQEALDTFLENQYERILKEGEIEPGGMGRLDKIDQIDPP
                     KFSLIVPLAPTVELNDYREIREEYVEAAITDEEVREILDELKTNYATAEPAEGPAKEG
                     QTVYVLIKADLKEEDPETGEKELIKERPYEFIIGKDSDEKTAWPFVNFSQNFIGAQPN
                     EIVTVEHTYGEEAPIPTLKNKTVVYTTTVQSIKDLVMPAEDLELAKKFGDYETFDDFL
                     KDIRERLLKQKQQSQENEYIEKIVKQLVEKADIQYAPNALEEEAQEIFSDFKQRLAQQ
                     GVDLETYLKMKNTDMEKFLNEEIRPSAEEQLKRRLVLQEFAAKEKIRINMDNFKENVG
                     KLQETAAIDYQKAKTNKEKQAIANYITNAAMNQSFNDALFDRLIAIAKGENPAIEEEE
                     TTPESEKETAVDNLETVQNETESSNDLPVEIGSNEKIEMPESETDSENKS"
     gene            26175..28097
                     /gene="ftsH"
                     /locus_tag="PK898_03675"
     CDS             26175..28097
                     /gene="ftsH"
                     /locus_tag="PK898_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU19164.1"
                     /translation="MSQSNNTQKNRNDKNRSGQNQLNYVLSYLIFAFLCMWLFQQFII
                     KPITVRETEITYSEFKSKIKSKEVSDVLLGEYRIVGMMKNPNATSAEDEKIPFTTVAM
                     PNGDPTLIELLDENGINYTISEPANPLGSIILGYLIPLLFFGVIWYLISRQVEKSGGS
                     FGGLGGIMSIGQSKATEVKPEDVNVTFKDVGGADEAIEELQDIIQFLKEPEQFSKLGG
                     RIPKGVLLVGPPGTGKTLLAKATAGEAHVPFFETSGSEFVEMFVGVGAARVRNLFDQA
                     RKVAPAIIFIDEIDAIGQSRVGPGRFGSNDEREQTLNQLLSEIDGFKSDKGKPVIIMA
                     ATNRPEILDPALLRAGRFDRQVTVSIPDLIGRKQILQIHSKNIKLDPSFDLDRAARMT
                     PGFSGADLANVMNEAALLAARRNGNSVNMDDFELAIERIVAGLEKKTRVMNEKERTAV
                     AYHESGHALIGEIVPNGDPVAKVSIVPRGRGALGYTMQMPTEDRYLMTQSELEDKIAV
                     LLGGRAAESIIFNEISTGAADDIKKATELARRMVTEFGMSEKLGVVRYATPQYQFLES
                     YETAAVASPETLKLIDEEVQRILDEQFKRVINLLNENRDALESLTKLLLEKETISGEE
                     VKEAIAKQRIKEPAIV"
     gene            28275..28874
                     /locus_tag="PK898_03680"
     CDS             28275..28874
                     /locus_tag="PK898_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOU19165.1"
                     /translation="MDKPRNAVVPMVVEGGAYGERAYDIYSLLLKERIVFLGTQIDDQ
                     IANLTIAQLLYLSREDPDRDIQFYINSPGGSVYAGLAIYDTMQMIPNRINTVACGVTA
                     SFGTVLLTAGSKGHRFALPNSTIHMHQPLGGVEGQASDIEIQAKEILRLKKELNEIMA
                     KHTGQTYETILHDTDRDRYFTPEEAVAYGLIDKVLEPTK"
     gene            29094..29930
                     /locus_tag="PK898_03685"
     CDS             29094..29930
                     /locus_tag="PK898_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOU19166.1"
                     /translation="MEVFDFSKIKSIFLDMDGVLWNGKNPIGNTRDIFTKLDQADIFP
                     VFITNNSTRTPESYQEKMAGFGVKIKPDQVITPAVAASYLFKKNYSKDVRIYVFGSQA
                     LKDYLKREGFTLSDEKADIVLVSLDQEMTYQKVATAMRLINEGAIFYATNRDHILASE
                     NGWLPGGGVMVSAVETCTQKKPIVIGKPQPYMIEIAMEKFGLDKSEVVAVGDRYDTDI
                     IGGINAGCHTVMVLSGVDTKESVSSFQKQPTLICKDLEEFAEILIRQKNNLDPINRTD
                     KL"
     gene            29927..30958
                     /gene="rlmN"
                     /locus_tag="PK898_03690"
     CDS             29927..30958
                     /gene="rlmN"
                     /locus_tag="PK898_03690"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU19167.1"
                     /translation="MKTCFFDLNYEELLSILKEWQEPEYRAKQLWNLVYRNLNFVPQN
                     FSNIPKDLREKLDETFEFQPIKIIRNVSSSNQQANKYLLGLTDGKKIEAVLMRYRERT
                     TICISTQVGCAMNCAFCATGQMGFTRNLTSGEIAGQVFFFVKELEKEDRKLTNLVLMG
                     MGEPFHNYANVMKGIDLINDPEGFNFGERRITISTVGIIPMIEKFTKAKRQINLAVSL
                     HAPTNTIRNQIIPINKKYPIEDLLAACKAYTDYTGRRITFEYAMISGLNDSIEQAVQL
                     SKLLRGMLCHVNLISLNPTRKYDRTGSSRESINNFYSILDKAGIPCSIRLNRGVDIGA
                     GCGQLLAQA"
     gene            31012..31929
                     /gene="ftsY"
                     /locus_tag="PK898_03695"
     CDS             31012..31929
                     /gene="ftsY"
                     /locus_tag="PK898_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOU19168.1"
                     /translation="MAEFLSKWMKGLDKTRKVTFSRLASVLGTNEIDEYLWDDLEAIL
                     IQADIGVETSNEIIESLKKTVLDQGLYKSSDLVAYLKNELIQRLPESRDPLDESEKIK
                     PYVILMVGVNGSGKTTTTAKLAKKYTDAGKKVMIAAGDTYRAAAIEQLQVWGNRLNIP
                     VIAGKEGGDSAAVAFDAVQAAIARNMDILIIDTAGRLQSRYNLMEELKKVHRVIGKAL
                     PGAPHAVWIVLDATTGQNALIQAKAFKEAVKVNGVILAKLDNSAKGGMAFAITQELDL
                     PIIYAGLGEKADDLERFNREDFVNGIIGD"
     gene            31939..33018
                     /gene="prfB"
                     /locus_tag="PK898_03700"
     CDS             31939..33018
                     /gene="prfB"
                     /locus_tag="PK898_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOU19169.1"
                     /translation="MQESIIKMNSIKQKLNELTAHLDLETKKKHLSDLQKESQKPDLW
                     DNQDKAQNLLKEINDLQDEISDWEGLSEKVDDTIELLEIGDTSIGEELQNDINSIEKE
                     IRQKEIQVLLSGKYDSGNAILAIHAGAGGTEAHDWAAMLQRMYLRWAETHHYAADIVD
                     FTPGEEAGTKTITMTITGKYAYGYLKSEKGTHRLVRLSPFDAAHRRHTSFAMVEVLPE
                     APDTEEINIPPEDIRIDVFKSSGAGGQNVQKNSTAVRLLHIPTGIIVTCQNERSQTQN
                     RENAMKVLKSRLLEIKHQEHEKELGELRGDYVKAEWGSQIRSYVLHPYQMVKDHRTDY
                     EVGNAQAVLDGDLDGFMEAYLNYKK"
     gene            33084..33521
                     /gene="mscL"
                     /locus_tag="PK898_03705"
     CDS             33084..33521
                     /gene="mscL"
                     /locus_tag="PK898_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOU19170.1"
                     /translation="MKKTFSEFYEFIAKGNVIGLAIGVIMGTAFSAIVNSLINDVLMP
                     FIGLLTSGVDFKSWFIPLNGQSYASLKEAQEAAAPVITIGNFIAAVINFLIIAVILFF
                     IMKAMAKAMHTEKKEEPAPVITEPSEEIKLLTQIRDALVKNNG"
     gene            33787..34839
                     /locus_tag="PK898_03710"
     CDS             33787..34839
                     /locus_tag="PK898_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19171.1"
                     /translation="MKRSLIFTFALLILAIAAPVFAQSGELNVLCTPQELWCQGMEEA
                     FEAKYPEIDVQWIRLSSGEGLARVQSEAGNPTFDIWWGGPMDTFIAAKPLGLLEQYES
                     PNLKNIIPEYYDLFVDKDHYYTGIYMGSLGFITNTDWLAENPGVEAPTSFEDLLKPEF
                     KGQIVISHPSTAGTAYTFLSTVLQVMGEEKGWEYLKKFNEQVAIYTKSGSGAGKYVAN
                     GEFAVAIMFSHDGVKEIEDNGAHLVITFPKEGTGYEVGAEAILKGAKNLENAKLWYDW
                     ALTPEAQSLGPKFQSYQAPTVTGVELSHPELLQVKLIDYDFQWSGAHKQEFQDKFANE
                     IQNADMDESGVLIKQP"
     gene            34947..37358
                     /locus_tag="PK898_03715"
     CDS             34947..37358
                     /locus_tag="PK898_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU19172.1"
                     /translation="MTVNSDSIPPKTRFQGIKRRIHEFGLIAQDPILMIALIFSIIIL
                     TIFIFFPIFRTIIRGFVSDTGEADFTHFARYLDGTFLQGINTFFKNAGLILQETATYG
                     RTYRLVLSNTIKMGIFSATFGTFLGFIFAYATVRCNIPGKKFVHLLALVPTVSPPFAV
                     AIAMIQMFGRNGLITKTLLGIKFTPGMNDIYGMDGLVIVQTLTFFPIAYLMLESMMER
                     LDASMEEAAETLGASRFYLFRTITLPLLIPGIASSFLLLFVESLADLGNPQFISGNVT
                     VLSSQIYMSVIGEFNYQKASALSFVLLLPTLIVYIVQRYYITRRSYVSVTGKPTVGRV
                     YEKNKTIRIIFATMVYLVCILILLVYITVIASSFVKGWGIDFTPTLSNWTKMFGRGLE
                     AIFDTTFLSLYATPFAALLGMIIAFLIVRKKFAGKEILDFGSNLGGAVPGTIVGMGFI
                     LAFNFNNPGLAVFLIIVCSWFYITCVSKANFQRVKILIPGIILGLIMSCLDDGGFLWG
                     KNGWLTGPVSAESQAPAAFGIGLNGIYYLIGAIFILIGFYFLLQKNYRKLGKYSFFIG
                     IYIILNKVILIISKPIRIYGKTQSVRWWKAVCTQFADYIEVPFSLPTFFLGVCLFFIT
                     AIMIYEYHGKFETFFKTLFMILSIAILFTGDQMKMNGTAYIILFAFLIRSLPASVRSG
                     VAALQQIDGSIEEASNILGGDSQYTFRKVTLPLILPAFMTGLIFSFTRHMTSLSAIIF
                     LVSAKWQIVTASIMSGWEQDGISYAATYSSVIIFIVLIFIGLMGVVTRKVLKIDTNID
                     LNAGF"
     gene            37375..38451
                     /locus_tag="PK898_03720"
     CDS             37375..38451
                     /locus_tag="PK898_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19173.1"
                     /translation="MYLVIDNVVKVFPARGGAKEVRALDGITIEINKGELVTLLGPSG
                     CGKTTTLRMVAGFEFPTSGNIILDGKIINDVPTHKRDMSMVFQSYAIFPHLNVYENIA
                     YGLNVQKMSKEEQKKRIDAVLEMVHLEGYETRAPNQLSGGQQQRVALARALVMEPKVL
                     LMDEPLSNLDTKLREKMRFEIRQLQRQLGFTCLYVTHDQLEAMVLSDKVVVMNNGRIE
                     QIGKPRDIYRYPKTKFVADFIGRANFLEGKIIEKRNDELIIMVEGNPLTINDIHYNEF
                     TIGEEVEIIVRPEMIQVRTEEGKYSATIKQAAYLGESIEYILDFNGNELTAHETDPSR
                     LEIIPEGSSVKISFMEDCLHILKK"
     gene            38637..39431
                     /locus_tag="PK898_03725"
     CDS             38637..39431
                     /locus_tag="PK898_03725"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19174.1"
                     /translation="MNRKLIVILFVLLLPACTNMPQDMKESIYTEAAQTLIIQLTETA
                     AAVTPSETATVIPSSTPTEEPTVTTAPIVIPTIASNVTVAPTTSTADNPYKAEFVWVD
                     PYPNQFIPGQKFTLTWQLKNVGTATWSGKYHFYHNKGIQLADQSSYSIDTVVAPGEIL
                     TISMPATAPSTFGTYQTEWAFANPEGVQFYYVYYTAIVGEQTFVTDQPGAPTSTPNSL
                     YWMCSDPDRSNIQGNGCEEYCHLNGRSMDQDGVKCYSFGNLITDFY"
     gene            39509..40501
                     /locus_tag="PK898_03730"
     CDS             39509..40501
                     /locus_tag="PK898_03730"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19175.1"
                     /translation="MLKRKSLLITVLCTMLAFTACSNTPNKVVQLNTEAAATAAEIIT
                     QTAAAITPTDTAVPPTETPNYTATPIILPTILRYAETVAIATVVSGQPATGLYNGGEL
                     IGFSPASNIQVTPKQAFYLSVQIKNTGTTYWNTSYKIIHSGGTQMSYATSYPLTGDVA
                     SGGTYTASIYMTAPENYGTYTQTWTLVDAYNATVLTFSYNVIVGTTSVITSVPTLTPT
                     ITLTSQATAYSSQLDYMCSDAARSIEQGQGCEDYCKIANPYKSPCYYRGTINPTATNV
                     PSATLQPTKTYTTAPTAAPTSTPIPTSTTVPPTDTAVPTEETPSQQDGSADQNT"
BASE COUNT        12292 a         8363 c         8389 g        11552 t
ORIGIN      
        1 ggtgaggaac tgcagatacc ggtctttctt tatggtgagg ctgcgataat cccttttcac
       61 cgaaaacttt caaatttacg gcgaggaggt ttcgaaaaat tacgggaatt ggtcggtacg
      121 acaccggatt atcaaccgga tttcggaccc gcttatcttg gacccgcagg agcctgtgcg
      181 gtgggcgctc gttttttctt gattgctttc aacatttatt taagcacttc agatgtcgga
      241 gtggcaaaaa agattgctcg agaaattcga gaatcatccg gcggattacc aaaggttcaa
      301 gcattgggaa tgctcgtcaa caatctggct caagtgtcta tgaatctgac agattaccgc
      361 attaccccca tagacacagt ttatgaagcg gtagaaaaat cagcctatca aaaaggaatc
      421 acaatagatc actcggaatt agtgggatgt atcccaaaag ctgctattca gggaatagat
      481 cccgattcca taaaattgaa agatttttct ccggatcgaa ttctcgaaaa tcatcttcca
      541 tagcttaata tgttccgata aatatatttt atttaccctg atgcgcgagt aggttcttcc
      601 ggacaaattt tcaattattg ttcagcggtt actgttttgc atcgaaataa atataatcaa
      661 cttattccga taatatctga taaataaata atcgggcgta ttgactttct tatgcaaatt
      721 aaacgtcaaa taataaatat ttccgacatt ttagtatata atccatactg atatggagaa
      781 aaaaatgaca gaacaaaggt taccgtctta tggagggcaa gcgcttatcg aaggtgtctt
      841 gatgagagga gccaaccatg tagtagcttc gatgcgtgct cccgatggac atatcgaagt
      901 tgtgcatgaa gagttgggag gaatctataa aagcaggatt cgaaatatcc cgggactgag
      961 agggcttatc agtttatggg attctctggg tttgggctta cggttcctca caatatcagc
     1021 caacatacaa accggcgaag atgagaagat tgaaggacct gaaatggtca taaccatgat
     1081 tatttccatc gtgattgctg ttcttttatt cttccttgct ccggtatgga ttgcccattg
     1141 gattgaatca atgaccaatc tcacgcaact gggcgcaaat attattgaag gttttttgcg
     1201 ccttgccatt gtattgggat atatggtgat agtttctcgg atggaagaaa ttaaacgagt
     1261 gttcagatat catggtgccg agcataaaac aatcaactgc tttgataacg gccttgatgt
     1321 gacacttgaa aatgtgagga ctcaatccag attgcacccg cgctgcggaa ccgcttttat
     1381 ggtcacagta gtgttgattt ccatcattct gtttatagta atcggaccgt ttaccaagac
     1441 attgacaagc cgcttaatca gtcgagtgtt gttgattccg gtaatcagcg gaattgctta
     1501 tgaatatatt cgctggagtg caagacattt aaataatccg cttgttaaaa tcctgattta
     1561 tccgaattta atggttcaaa aaattacaac agctgaccct gacgattcga tgcttgaatg
     1621 tgcgatcagt tctttcttga caatgttggc ttatgaaaac ggaaccatgc cggttgaggt
     1681 aaacacgcct attaagacgg cggaatatgc aacaataggg tcggaaaaga caatccctta
     1741 aaattgaacg ggtagttatg tgaacaaatg gcaaattctc gtctcaggaa ttttgatcgg
     1801 tttccttttt agcgcactga ttattttatt tgcggatcaa caaaaaggaa agccgatttt
     1861 gttacaggcg gaatcgactc ccggcacgat tcaatttgaa gttttaggtg cggttagaaa
     1921 tccggggatt tactcatcgg aatcaattat acgggttatc gatgccgtaa atcttgcggg
     1981 cggacttacg gaaaatgccg atgcgcaaaa cagtaatctg accgaaaaaa tatatgatac
     2041 cgaccggatc atcattccga cgatcagcct cgttcaagcg acaacaacat ctatcgcaac
     2101 aatcaataat caaaaaagaa ttaatctcaa taccgcaagt ttggatgaat taatgctgct
     2161 tcccggaatc ggagaaaaaa aagcaaaaga tatcatcgaa tatcgaacaa atattcaacc
     2221 tttctcgagc gtttcggatt tattaaatat taacggcttc ggagaaaaaa caatcgaaga
     2281 attttatgat caggtcaccg tagaatcggg aggataaaaa tgggaatcaa aaatgatctt
     2341 gaaaataagt taaaagatgc catgcgcgag cagaatgaag ataaaaaaag agtgtatcga
     2401 atggcacttt catcaatcag atttgaggaa aaaacaaaga acaaagaact ggatgaagag
     2461 agtgtccaag cgattattca gaaagaaata aaaattcgca acgaaatgat cgaagatgca
     2521 atgaaagcgg gacgggatga aactgttaat gaaaaaaaag ccgaaatcag cttactggaa
     2581 gaattcctac ccaaacagct gaatgatgaa gaactttctc ggcttataag tgaaactgtt
     2641 caagaagttc aggccacggg accttcggat atgggaaaag ttatgaaaac cttgttgcca
     2701 aaagtgaaag ggttatgttc taatgagaaa gtcagtaagg cagtaaagga ttatttagca
     2761 aacctatgac ggaaaacgga ggagcagaaa gcaggttttc aaaagacaca gcctacttaa
     2821 gaggagtgat cttttgtatt accgttttaa tcacctttgc gctcctcact tttcagattt
     2881 ctgtcagaaa aacaagttac agcctgagtg tcggggatgt cgtcaatcag gatatagtcg
     2941 caccgcggac cattactttt gagagccaaa ttctcaccga acaagcgaga aatgatgctc
     3001 aaaatatggt ggataaaatc tatcttcctg ccgacccgac gatttccagg aatcaggttc
     3061 aatatctccg tttatcgtta caattcatat ccgcaatccg cagcgatcaa tacgctaccg
     3121 aagctcaaaa aatcgaggat atccagaaac tgatattcgt tcgtctggat gaaccgacta
     3181 ttaagcagat cctggaattg gatgactccg aatggaaatt agtacaatct gaatgccaac
     3241 ggattttaga gcaggtcatg caaaattcca ttcgtgatga tcaagtcaat tcggtaattg
     3301 aaaacatccc gacaatgatc agttattcga tcaatcctaa aaccgccgat ttaattaaca
     3361 gtattactcg gcgttttgtg gtaccaaaca gcctttacag tgcggaatta accgagaaaa
     3421 aacgacagga agcaagggat gccgtcgaac cgcgggaaag gacttttatc gcaaatcaga
     3481 cggttgtatc cagaggtcag ataattaccg aattaatcta tgaagcgtta gataaaatgg
     3541 ggctggtgca ctctaagaac gataatcgaa aaatcctttc atcggggctg cttgttctcg
     3601 gtctgaccgt ctttgctctc tatttttttc tgatcgatca tcgtcatcac caattaaatc
     3661 tgcgcgaatg tattttaatt gcgggtctct atttagcatt tctttttgcc gccagattcc
     3721 tcattcctaa tcgaacaatt ctgccattca tttttccgat tcaaaccttg gggctcacaa
     3781 tttcttgttt aagcggacaa gccttcggaa ttgtgatgtc cattattatc ggattgctcg
     3841 tgccttatga cttccccaac gcgctcggat acgcttcttt ttatatcatt tcatctttga
     3901 ccggtatttt agtccttcga aaagcacgac aagtaacgga ttttctgctt gcaggtttag
     3961 ctgccggctc gatcggaatc ccaattatca tcgcttttca ggttgccgag tctccgatgg
     4021 atatgatcgg ggttgtcact ctggcagctg catcggtagg cagcggtttg ttgtccaccg
     4081 gttttacgct tatttttcaa tattcattct caaaatttat cggggttgcc tcatcgctcg
     4141 aacttttaga gctgattcgt ccggattccc cgttgctgca atacatctta aataccgctc
     4201 ccggaacata tcaacattca ttgatggtcg caaatctttc tgagcaagcc gcaaaagaga
     4261 ttgatgccga tcctcttctt gtacgtgccg gatcgatgta tcatgatgcc gggaaagcat
     4321 taaatccgtc ttttttcatt gaaaatcagg taaacggatc gatcaactta catgaagata
     4381 tttcaccgaa agaaagcgcg gcatccatca tccaacatgt caatgacgga gtgatgttgg
     4441 tcgaaaaata tcgattaccc gaagtcatta aagattttgt ccgcgaacat cacgggacga
     4501 atgtcgcgag atatcaatac accgaagcgg taaaccagga aggggaagaa gcgatcgaca
     4561 taaatgactt tacttatccc ggacccaaac cgcaaacgaa agaaactgca ctggttatgc
     4621 ttgcggattc ttgtgaagca agggctcgcg gagacaggcc caagaatgat gaagaaatag
     4681 aagcactcgt aaaaagtgtt ttcgattact atagttcaag cggtcaactg gatgacgcac
     4741 ctttaacact gcgggaccta aagaagattc gcgagtcttt tgtccgagtt ttgcgcaatt
     4801 catatcatcc cagagtaaaa taccccgaac aaaaaaagga atcaaagaat aaaaccgcat
     4861 ccgttgtgaa aaataaccgc gagaacagaa ccgggaagac tgcagaattt gaatccgcag
     4921 aggcaaatat tcaaagggat aaatagtcat catgtttatt gacaatcgaa caaaaaaggc
     4981 tgatttagcg atcgattcat tcaaattaga tcaaattgcc gaatctgttt tatcggactt
     5041 tctggaacaa cccgcccaat gcacgctatc atttgtaaaa ccgtccgaaa tcagaaaatt
     5101 aaataaacaa tatcggggcg ttgacgaaat taccgatgtt ttaacctttc aatcggaagg
     5161 ggagatcgat cccgagacga acatacctta tttgggggat attttgattt gtatcgagag
     5221 agcttccgaa caggctgaaa tatccggtca ttctcttgaa aatgaagtcg aattattatt
     5281 gatccatggg ttgcttcatt taatcggatt tgaccatgat acacctgcga ataaagatct
     5341 tatgtgggaa aagcaaacag attatctgaa taagtttaac attcaccttg gaagaaaacc
     5401 gggagatgat tttgagttct aagtttattc gaaataggaa tgtttctttt cgaaatgcat
     5461 ttaacggaat tattcatggt ttcgttgatg agattaattt taaaattcat ctctttttta
     5521 cgattttagc tgttttatta agcgttattc taaagatttc tttgactgaa tggtgcctga
     5581 ttatcattgt catcggaatg gtttggtcga cggaattgat taatacggca atcgaacggg
     5641 cggtagattt atacacattg gaaaaacatc ctcttgcaaa acaaagcaaa gacatcgcag
     5701 ccggagctgt attaatatct gcgattacaa gtgttatgat cggagtaata attttccttc
     5761 cgaagattct catacaattt ttttaaattt cgttttattg actcatcatt ttgattcata
     5821 aatcaagcaa cgcagtcact attttgtaaa aatatgttac aatttgggcg tttgatttgc
     5881 cgaaaggcaa tctttccatt accgattatc gatatcggta acaatcccaa ggaaaggagg
     5941 aattagcaat gcgaaaatac gaaatcgtaa tcattgccaa tcctgatctc gatgaagaat
     6001 ccataaacgg aattgtcgaa aaagtcagtg gctggataaa agacagcggc ggtgaaatag
     6061 aaaaagtgga tgactgggga aaacgccgcc tcgcttatcg tattcagaaa cagcgtgaag
     6121 gacattatgt tctgataacc gcgaatattg aaccttcagc aatgaaagaa atttcaaata
     6181 atttgcgatt tgttgaatcg gttcttcgat cgatgatcac attagtcgaa gaataaaatt
     6241 tttatcttta ccggtgatct cgaccggttg caggagtaat tatgagtgaa gaaataaata
     6301 cggagcaaat ggatcacaga ggaaatcgaa ggtttattgc cagaccaaaa ttctgtcagt
     6361 tttgctcaga aaaaaatacc aaaatagatt ataaaaatgc agatctgctg cggcgctata
     6421 ttaccgaaga aggtaaaatt cgtccccgcc gtcaaacagg aacctgtgca aagcatcaac
     6481 gacaaatcgc tgccgcggta aagagagcac gttatatagc gttgctgcct tacacttccg
     6541 aaccttacca agacgttgtt cgttaaggat tcatccgcca aagaaagaat agggcatagg
     6601 gaagttctct gtgccctatt ttgctaagga gttaataaat tatgccgaat caagaagaaa
     6661 aaaaagaaaa tacgcatcct ttgctcttga acaaagaaag aaaatcgcga cacgaaaaag
     6721 aaatgaagca aatgaaatgg ttaaaaatag gcggaattac cgtctttgcc attgtcattt
     6781 tggtattggt tttcggtgca ttttatgaat atcaatatct gccgaataag acgatcgcaa
     6841 aagttcaaga caaaaaaata accgtaaagg aatttcaaga agctgtccgt tatcaaagag
     6901 tgaatttgat cagcaattat aattacatgg ctcaacttta ccaatccttt gggatgcaaa
     6961 tggatgaaga gacaaagtct caatatgaga cacagctatc cgaaccatat gcaggtattc
     7021 tcggccaaca agtttacaat acgatggtta atcaattaat cttggaatat ggggcaaaag
     7081 cagccggaat taccgtaagt gatgccgaag tagacaatga gattcaatcc atgtggggtt
     7141 attatcccaa cggaactccc actccaagac cttccgaaac accctttgcg aatacaccga
     7201 ccgtatccga agagcaactg aagctattaa attacacgga aaccccgact ccgacaactg
     7261 aaataaccga ccttacctcc ttgaaagaaa gcggtcccga agcgaataca ccggttgtaa
     7321 cggatactcc gactttaagc cctaccgcaa ctcagacaga ggtcagcagt gaaagcccga
     7381 ctcccgtttt gagtccgacc ccgagcttaa ccccaaccac gtatacggaa gagatgttcc
     7441 aaaataattt caataggcag tttgaaaata acccctatta ttccaaggaa tttttccgaa
     7501 agcaggttta ctatcagctt ctcgaacaaa aagtaagaga tatgctcgca aaagatattg
     7561 agcccgaagc ggagatggtt tgggccagac atatcttagt ttccacggaa gatgaagcca
     7621 aaaaagttat cgagcggttg aataacggcg aagattggaa tgcattggcg gcagaagtct
     7681 ctacagatac atcgaataat accaagggtg gagatttggg ttggtttatg aaaggtgtca
     7741 tggatccgga ttttgaaaag gctgctttcg aacaagaagt tggcacatat agcaaggaac
     7801 cggttaagac acaatatgga tatcacgtta ttcaagtggt cggtcacgaa gtgagaccgt
     7861 taaccagcac cgatcgatcg aatgcaatcg acagagctta taaaaattgg ttggaaaatg
     7921 cgaaaagcaa tttgaaaatt gaaaccggat cggattgggt tgactttgtt ccggtagagc
     7981 cgacgttatc tgatttataa tcttttcata tcaccagaaa caaaaacacc cgatttctcg
     8041 ggtgtttttg tttctggtgg tcaatcgaaa aaacaaataa tacttagact taggattcgt
     8101 cctttttatc tttcttggct ctcacttctg tcttcctttt aggttgaata ttcccaaaag
     8161 ccaagggaat aatttgatcc atgtggttaa ccgaagtaat tgtcaattct tctttgatct
     8221 gttgaggaag ttcctcgaga tctttctcgt ttttttcggg aataataatc gttttgattc
     8281 ctaatcggtg agcggctaac accttttctt ttataccgcc aaccggcaat acattcccgc
     8341 gaagcgttac ttcaccggtc atcgcaatat ctttttttac gggcgtattg gtgatagcgg
     8401 aaatcaaagc aatagctaaa gtcactcccg cactcggacc gtctttcgga accgctcctt
     8461 ccggaatatg gatatgcaca tccgttttct caaaatagtc tttgggaata ttaaattgtt
     8521 ttgctcgcga tttcacaaat gacaaagcag cttgggctga ttcctgcatc acatcaccga
     8581 tttgtcccgt aacctgcaaa ttcccttttc cgtcaaaaac caatacttca atcgtggtaa
     8641 tatcgccgcc gttcggtgtc cacgccattc cggtcgcgac tccgacctca tcttcctttt
     8701 ccttttcgga ttggaaaaag taaggaggtc ccatatattt tccgacggat tcagccgata
     8761 tttctcgctc gtattctttt ccttcagcct ttaatctggc tattttccgg catattttgc
     8821 cgatttcccg ttcgaggttt ctgacacctg cctcataggt atattcccga atgattctct
     8881 taatggcatc atctgtgaaa ttgatatcct gaggctctaa tccattttct tccgtctgac
     8941 ggttaatcaa ataatctttg gcaatgatta atttttcttc ttcgatatat ccggggaatt
     9001 caatcaactc catccgatcc aacaacgcat ccggaatcat ttcaatcgaa tttgcagtcg
     9061 taataaacat tactttcgag agatcgtaat ctacttccaa ataatggtcc gagaaacgac
     9121 tattttgctc cggatcgagt acttcaagca atgcagcaga cggatctccg cgataatcat
     9181 taccgagttt gtcaatctca tccaacataa acaacgggtt gatcgtcccc gcttttttca
     9241 tggtttgtaa aattctgccg ggaagagcac cgatatatgt ccttcgatgt ccccttattt
     9301 cagcttcgtc tcttactcca ccgagagaaa ttctgacaaa cttttttcct aacgcttctg
     9361 cgatagatcg gccgattgat gtcttccccg ttccgggagg gccgacaaag cataaaatgg
     9421 gttgtttctc tttcttcggc tttaaggaac gcaccgcaat atattccaat atccgatctt
     9481 tcgctttttc caaaccgtaa tgattcttgt tcagaatttc cgaagcatgt ttcacatcca
     9541 ggttatcttc cgtctgttcc acccacggta aatctaaaag ccagtcaata taacttctgg
     9601 atacattgac ctcaggggac attgccggaa gttgtgtcaa tctttcaaat tctttgaggg
     9661 cgacttcacg tgcctcgtca ctgaattgtt tttgatcaat cttctctttc agttcattta
     9721 agtcacggct gaagacatca tcttcattta attctttttg aattgccctc atttgttccc
     9781 gcagataaaa ctcacgttga tttttatcca cttcattctg aaccgttgta ctgatttggt
     9841 ttccgagttc caatacatcc aattcttgtg ccagaaagct gtttaagatt tcaagtcgct
     9901 tttccggatc caattcaaaa aggatttcct gtctttgttc gttctcaaaa gaaatggtgg
     9961 ttgcgatcat atcagccaac catgacggat cagtaattgt ggaagcataa gcgaccgctt
    10021 catccggcaa tttgcgatcc aattggacac atttttcaaa aaggttccgg gtagtccgga
    10081 tcaacgcctc cagatgtctg gatttctttg cttcttcttc cgatacataa acccgtgcca
    10141 taaggaccgg cgatcgccgg acaatatcca tcagtatgac ccgtcgtctg ccttgtacta
    10201 acgtactgaa atgttcttcc ggtaaattga ctaaacgtcc aaccgcaatt tccagcccga
    10261 ccggataaaa atcggatttc ttgaccggaa attccttttc cggatccttt ggaattaatg
    10321 taattacggt ttgttcattt tctaaaccat atcgtaagga ttcaagattt ttttgttggc
    10381 cgacgaatac cggcgttatc atttttggaa aaatcacatt ttctcttaaa acgacaaccg
    10441 gcgcgacaaa acaaccatcc gaatccaatt ccatatccgg aattcgaaac agttcctccg
    10501 tcctttgatt gatattcgtc gtaaaatcat tgacaagatc tttatcttct aaccaattac
    10561 ttgaactcat ttaaactcca aatgttattt tttcttttca aaatacttca tatcgggatt
    10621 acgcttcatt aaaagttcgc gaatccccgc cgtaacaaaa aggctgcccg taacaatata
    10681 aacattattt ttatcaccgc tgtctatatc gcgtaaagcg agttccaaag attcccgaat
    10741 gacagccggt ctgccgtttg aaagcacttc atcataaatt tgttttgttt ccattgatct
    10801 tggatgagtt gaacgggtaa caataaaatc actgacaaac ggcgcaagta tcctgatcat
    10861 tccgcttaaa tctttatcct cggaacaacc gaaaacacag gttatcctct ttgtcccata
    10921 aaaacgattc aatgtcatga caagtttttc aatcgaatcc ggattatgcg ccccgtctaa
    10981 aataaccggc ggatcaatcg atactttctc aaacctgcat ggccaaaaag tcttcgctaa
    11041 tccatcgctg atacttttct catttatttt atccgcaaaa gatctgattt tcattaacgc
    11101 tgtgaatgcg atcacggcat tgatagattg atgatatccc gcaagcggaa tatcaacttc
    11161 ccaaggtttc catatactct tttctctatt ttccagccaa ttccgcaatg cattttgttc
    11221 cttacgctcc cagatcatca gtctctgccc gtccacggaa tcctttaatg gaacaaagcc
    11281 ataattttta tccacatcaa tccattcggc atttctttct tcggctattt ttcggatcac
    11341 ttccatagct ttcggatctt gagcagctgc gattaaagga acattctgct taataatgcc
    11401 gcctttttcg gtggcgattt tctcgatagt gtccccgaga aaattcatat gatccatcga
    11461 aattgaggta attaccgtca aaaccggatg gacaacattc gttgaatcca atcttccacc
    11521 taatccggtt tcgacaacgg catattccac tttctgttcg cgaaagtatt caaaagctat
    11581 cgctgtcatc atatcgaacg aactgggcgg gttggcagga tcgattttct caataacttt
    11641 ctctgtcaat tgaatcaatt ctgcgtctga tatcatcttc ccatccactt gaatccgttc
    11701 attaaagtga atcaagtgcg gagaagtgta aagtccggtt ttatatcccg atgctcgtaa
    11761 cccttcataa atcattgtgc aaaccgagcc cttgcctttt gttccggcaa tatgaatgca
    11821 tcgatatgct tcttgcgggt tattcaatcg gttcagaagc tctgtaattc gattcaaaga
    11881 aaaaactgcg gaggaaattt cctccgcatg tgtcatactg taatctacaa aggaatatat
    11941 tgcatcaagt gcatcatcaa tcgtttctaa atgagccaac atgctgctta ttttaccgtt
    12001 aattcgcttg tttttttata ataaacaact ttttgttagc cgattgattt ctttcgttgc
    12061 agccaataaa atattctggg gttcaaatca agctcggtat ctcgattacc ggcaatggta
    12121 aaatcaggct gaatcaaatc ggtataaatc ccgggttcaa agtcaagttg aaaatgcatc
    12181 ggatagtcgc tcatgttatg tatcacatac acaagatcat tttctgaacg tcgaatatat
    12241 gccaatactt ttacttcgct tccggtcgta atccattcta attctccttc tcccaacgct
    12301 tcggaagctt ttcgaatgcg aattaatctc ctcaaccaat tcaggtttga atccggatca
    12361 tcttcctgtt gcatcacatt tactttttga taaccatatt cctcatcggt aatcgcaggt
    12421 aaataagtgc gatcggccgt tgaaaatccg ccgtttttcg tatcatccca ttgcatcggt
    12481 gttcgcacac cgttccgatc aaataagtta atattatctc ccatcccgat ttcgtcgcca
    12541 taatacaaaa caggcgatcc cggtaaagta aaaatcaagg aaaatgccaa tccgatcttt
    12601 ttccgatcat tatccaacag cggatataat cttcgtcgaa tccccaagtt caagcgcatt
    12661 tctttttgcg gagcataaaa tgcccacatc cattgtcgat cctcttcgga taccatctcg
    12721 agcgtcaatt catcatgatt tcttaggaag gtactccatt gacaattttc cggaatctcc
    12781 ggcgtctctt gcataatttg caccaacgaa gaagcatcct gtttcccgat agacataaat
    12841 attctcggca ttaaaggaaa atgaaatccc aattgaaatt catcaccgtc tccgaagtat
    12901 tcccggacat cccgcggcca ttgattcgcc tcgcataata aaattgttcc cggaaattcc
    12961 agatccatca tttttctgat ttctttaaga tatgcgtgcg tttccggcaa attttcatta
    13021 atcgttcctt ctctctcaaa aaggtatggt acggcatccg cccgaaaccc gtcgatgccc
    13081 agattaagcc aataacgcat cacattcttc atttcttcgc gaacttcggg attgtcataa
    13141 tttagatccg gttgggaagg ataaaatcgg tgccaataat aagcccccgc ttcctcatcc
    13201 caagtccagt ttgatttttg tgaatcaacg aaaataattc gggtcccctg atatttcgta
    13261 tcggtatcgc tccagacaaa ataattgcga tatttcgaat ttggatcttt tctggcggat
    13321 tgaaaccaag gatgttgatc ggagcaatga ttgagtacca aatcagtaat aactctcatt
    13381 cctctttgat gaatctcatt aaagagttgt tttaagtcgt ccaatgttcc aaacggttca
    13441 aatactccat aaaaatcgga gatgtcatat ccgtcatctt tcagcgggga tttataggtc
    13501 ggcattaacc aaatacaatc cgtccccaaa ttccgcacat aatccagttt ttgaataatt
    13561 ccttgaaaat ctccgtatcc gtctccgttt gaatcataaa aagatcgggg ataaatttga
    13621 taaaatatcg catttttata ccatagagga ttgatattca caaaaactcc ttagtaagaa
    13681 ataagactct gacaaaatac gtacaattga tttcaaaaaa cgatccccgt aggatatttc
    13741 ttccatgcgg ggatgccaat aatctttgat tatcccttta ccgatcccgc catcatcccg
    13801 cgtacaaaat acttttgtaa ggagaagaat acggtcatcg gaataatcat cgagataaat
    13861 gcaccggctg tcagtaaatg ccaatcttga cctttggttc cgaccatatt tgccaaggtt
    13921 tgtgtaacga cctcatttgt tccgccgcct aagaccacca aagcgaccag gtagtcattc
    13981 caaacccaaa ggaattgaaa gatagcaaaa gatgcgattg ccgggacgga taatggcaga
    14041 attaatttgg taaagatcga aaaattcgtt gctccgtcaa taaatgccga ttcaagaata
    14101 tccctcggga tttcactgat gtaattaaat aacagataaa tcgccaatgg taacccaaac
    14161 cctgtatgtg ccagccagat tcccaagaaa gtcccattta aatggatccg ggaaaaatct
    14221 tgcaacaccg gaacaatagc gatttgaagc ggtaccacca acagcgctac aaccatcgta
    14281 aaaagaatct tacgcccggg aaaattcagc catgcaaatc cgtaggcggc aaatattgca
    14341 atggtaatcg gaataattgt cgccggtatc gttacggtta atgaattgat aaaagaatta
    14401 ctgagatccg atccgttacg ggtgagtaat tttccatctt cgcccttata ggtgatcttt
    14461 tttccgctta atacattgcg atagttttcc aaagttaaat ccgtttcaaa tccggtccaa
    14521 cgcctttcat aaatttttag tgtcctgctt cgtttggcgc cgtagtattg gattttttct
    14581 ccgctttttg tgataacacc gtttttccaa aaagaataat caccggaaat ttcctgtccg
    14641 tcttccgata caaacttgat ttcaccgttc agatcgatat cggcatcaag tttatattca
    14701 ccgatttcga cccattcttt atgcggaaaa acagtccacc aaccgctgtt gaaaattctt
    14761 tcagattgcc ggaaagatgt gatcaagatc ccaaaaatcg gaactacgaa gacagccgaa
    14821 attaagatga ggaccaaatt aacgataaaa gaagatgtct ttttctgact tttataactt
    14881 ttttcttttt gagccatcag aacaccttcc tttcattaaa ctgccggaga ttcatgatca
    14941 taaccggaac gaccagaatt agcagaacaa tcgcaatggc tgatgcacgt ccgatgttat
    15001 agtacaagaa acgttgatta tagaattcgt tggcaattac attggtcccg taattgcctc
    15061 ctgtcataga ttggacgaca tcaaatgtct tcagactgaa aatagcaacc gttgtggtaa
    15121 ccgcgatcaa tgatccttta atcaccggaa tgatgatttt gaaaaaaata gtggtctccg
    15181 atgcgccgtc tattcttgcc gcttccaaca actcttccgg aattccctta atagcggatg
    15241 aaaggatcac catacaatat ccggtttcca accaaatcat gatcacaatc agtagaatat
    15301 tattccaaaa cggaatttgc agccaggcat gtggttgtcc gccaaaggac ataacgatag
    15361 cgtttagcaa ccctgtttcc actccttgtc ccttgtagtt ataaataaat ttccaaatca
    15421 cgccggctcc gatgaatgaa atagccatgg gagtgaaaat gatggattta taaacttttt
    15481 catacttgga ccgatccgcc aaaaccgcta tcagcaaccc taaaaataca ctgaaaaaag
    15541 ttcccaaaat cagccagagc aaattattcc gaaatacgat cagcatttga tcatcggtaa
    15601 atgcatattt ataattctgc aatccgaccc aatttttggc atccgcatct tttaatgaac
    15661 tgattaaagt tcgaaatacc ggcaatgtta aagcccaaat caatataata actgccggac
    15721 cgacaaatat atacggttga atcctcgctg tccatttcgg cgaaaaatgt tctacaatcc
    15781 agttggctcc ggtataaaat aagacaactg tactgactcc ccaaacgatt gcaaataaag
    15841 cagagggtat tttaagatgg ttcgtgtcta aaaatgttat gattctgact ccggcagtaa
    15901 aaatcagaat aattcctgcc aaaatcagga tcattttgaa gaaaaggaca attttgctcg
    15961 ggcttttccc gaaagtttgt gttttattca tttaaccctc cttttctata caccatgggg
    16021 gtaaaaagaa tgatccgaat ttcaggttcg gtaccaagta agaaagaaca gaaacagggg
    16081 tgcctccgct ttcacggagg cactcattct tacaacaggt tcggtattat tgctcccagc
    16141 ccttatcgat atttttcaca actgtgtcaa tatctgtccc ggtaaccaag tcaaccatac
    16201 ctttccaaaa tgttccggat ccgaccgcag ccggcatttg atccgaaccg tcaaaactga
    16261 aagaggtcgc atcattaatc atttgcgata tcttcgcgtc ggtagcgttt gcgtaccaag
    16321 aaggatcggc atcttttccg gggaacatcg ctccgccgtt ggcaagccaa actttcaatg
    16381 attcgccgtg agtaaagaat tcaagggtag ctttgacttc atcacgatca tgtcctttga
    16441 aaacactgta tacatcgccg gcgcctaaga acggtcttcc gtattgctcg tcaatgggag
    16501 gaagataaaa cacatcataa tcgactcccg gtaccgcatc ttcagggaaa taagctgtga
    16561 taaagtttcc cagagaatac atcatacaac cgttggtatc ataaaaaagc ggtttgacgg
    16621 cttcaccgaa attcgttgtc gcaatcgctt ctcttccgcc ataaacaaat ccatcggtaa
    16681 agaagatttc gctcataatt tcaagtgcgc ggcgaacttc cgggctgtca aacttcagtt
    16741 cgccggttgt ccacttctta taattttcca gcgaagtggt ccgcagcatg atattttcga
    16801 cccaatctgt cgccggccaa ccggtccccg caccgccttc gattccaagg caccacggag
    16861 tttcaccatc ggcaaccatt tgtttggaaa gatctaccat ttcctgccaa gtttccggag
    16921 tttcataacc ggcttcttcc caaactttcg ggcgataata cacctgtgat ttggcgttgt
    16981 tacgagccca aattccaccc ataatcattt cgcctttttc attgggaacg gtcgccaaat
    17041 ccagccaggc ttgtgaataa ttttctttca accagtcttc ggatataaaa tcgcgaacgt
    17101 caacgatata tcctttttta gccagattag ccattccgcc gggttgaggg aaatccgcga
    17161 tatcgggggc gtttccgcct tcgacacgca tttggattgt tgattcaaaa tctttatttc
    17221 cgatatattg aatatcaatc cccgtttttt cttcgaaatc ttttatggat gcttcaaaca
    17281 atacttggtc attatcaatg aatggtccat ccagagtcac gatttttccc ttaaattcgc
    17341 catcataagc ccgctcaaga taggttttat cctgtgcgga gaccattgcg gcagctccga
    17401 acagcattgt aactgccaaa attaaagaaa atactttttt catttctcct cctgaaaata
    17461 aaatggttaa cacctttttg aggcgttatg aaattatgtc gtctttcgaa cgacaagttc
    17521 gaccggaagt tgaaccaact gtatcaacct ttccggatcc ttcaaacggt tgatcaataa
    17581 atctacggcg actttaccgc tatgatccaa attttgacga atggtcgtca gacctacata
    17641 atccgcaaag tcaatgtcgt caaaaccgat aacggcaaca tcctgaggga tatttaatcc
    17701 gacttctttg gcagcccgta aaattcccaa agcctgaacg tctgcagcgg caaagattgc
    17761 atcgggaaat tctcccgaac ggaatatttt cagaaaattc agataagtct gatccgtatc
    17821 acactttaca tcataaatat atcgggggtt taattcaaac ccatgactgt ttaacgtcga
    17881 ttgataacct tccagacgtc cggaaatagg ataaacgcta taatcgcttt tattcagttc
    17941 ggcggcaaat ccgaattttt ttctgccttt ttcaatcaaa tgttgagctg ccaattttcc
    18001 gcccattcga tcatcgatga caacacaatt cgttaaaggc gatgaatatt cgatcatcac
    18061 catctctatg ccgcactctt taattctttt gatttgttca tcggtaaaag ggatcgaaag
    18121 cgtaatcaag ccgttcagac cgcgtaattc caaggaatca atgaatcgat ccatctgaag
    18181 tttggaagca accgaataaa cctgcaattc ataatcctgc ccggcaagcg cgacggttat
    18241 tccgcgtagt cgttcaacaa atgaattctc ggtaaagcgc ggggcacaaa cgcaaattcg
    18301 ctcaaaatca tgcgccgtac gcaatcgcgc ttcaagcagc gggcggtatc ccagctcttg
    18361 aatagccatc atcacttttt ctttggtaca gggtttaact ttttccggat tatttaagac
    18421 acgggaaatc gtggccgtac tgacccctgc aagttctgat acatcataaa tcgtcggtgt
    18481 cttgcccaca tttccctttc cgttacaatg taactgctta cattgtaact tattacattt
    18541 taatttaatc catttattgg attaagtcaa attttttctt agagtaattt agtaaattaa
    18601 taagaaaatc atgccaattt agtgattttt gttttatcat tggataaaat caacacatga
    18661 tagaaaaaga gcttacccca aaggaatata aaacttgaaa ccgcaagaaa ttgaaaagga
    18721 aatcgaaaag tttttattaa aagtccaaaa acccggtcgc tatgtcggcg gagaatttaa
    18781 ttcgatttac aaagattcat cagcaataca gacgcgcatt gcgcttgcct ttcccgatat
    18841 ttacgacttg ggagttccca atctcggtat cgcaattttt tatgatctct taaataaact
    18901 tccgaatgtc tgggctgagc gtgtttatct tccttgggaa gatatggaaa aagagatgcg
    18961 cgaaagaaat atccccctgt atacgctgga aagtaaaacg cctgtaaatc agatggatat
    19021 tttaggtatc acaattcctt atgaatcgct ttatacaaac gtcctgaatc ttttggacct
    19081 gtccggttta tccgttttcg ccgccgaacg ttctgaagaa gatcctttgg ttatcgccgg
    19141 cggacattca acctataacc cggaaccgat gtcggcattt atcgatgcat tcgtcatcgg
    19201 agaaggagaa gaagtattcc ccgaaattgt cacagtatat cagctgtgga aaaaacaggc
    19261 attgccgaaa tcggaattgc tttatcggct ttcgaaagta gaaggggttt acgtcccgct
    19321 tttttatcaa acgacctata acggagacgg aacggtttcg gcaattaatc cggttaacga
    19381 tagctttccg aaacgaataa aaaaacgtat tctccccatt ttaccgcccc cgccggatta
    19441 ttttgttgtc ccatccatcg acgtggtcca aaaccgcatt gcgattgaaa tcatgcgagg
    19501 ctgcagtcgc ggatgccgat tttgccaagc gggttttgtg acgcgcccgg tacgggaaag
    19561 accggccgag caaattctgg aatccattga ctccgcattg tcttccaccg gctatgagga
    19621 gatcgcactt ctttcccttt cttcctccga ttacactcaa attcaaacgc tcgttgatgc
    19681 gctgcaaaat aggtacggca aaagaaagtt aaatatttcg ctgccttcct taagaattga
    19741 tacggtttcg gtaaatattt tggaaaaatt aaaaggatcc cgtttcagcg gatttaccct
    19801 cgcgccggaa gcagcttcgg aaaaaatgaa aaaaacgatt aataaaccga ttacagaaga
    19861 acaattgctt gaaaccgctg aaatgattta tagccacgga tggcagacaa ttaagttgta
    19921 tttcatgatt ggcttaccgg gagaaacaga tgaagatgtc ctggcaatct gtgatttatg
    19981 tttaaaagtg attagaatcg gacgaaagta tctcggtaaa cgctcacaat tgaacatagg
    20041 aatcgcgaca ttggttccga aacctcacac acctttccaa tggtggaaag ccgatgagcc
    20101 ggaaaatatt gcaagaaagc aggatttgct gaaaaagggt ctttccactc ccggcattaa
    20161 aattacctgg tcggatccgg atacaacgat gtttgaagct tggtgttccc ggggcgatcg
    20221 acggatgggc gcggttattc atcgagcctg gcaattgggc gcaaaatttg atgcgtggca
    20281 ggatcatttt aacaaggaag cttggctgca agctttttcc gaaaacgggt tggatccgga
    20341 tttttatgtt caccgggaaa gatcaaaaaa tgaggttttc ccctgggacc atatcgatat
    20401 cggtgtgaca aaacatactc ttcgtaaaga attcgaaaac ggtttagaag gtgtcctgcg
    20461 acccgattgc cggaccggtt gttacggttg cgggattaat caagcgtttc gtctggttca
    20521 accaaaagcc ggagaacaaa aatggttctg tccgatactc aatttttccg ccgaaaggga
    20581 attaaccgaa tgatccgttg tcgaatctcg tatcaaaaaa ccggcccgct tcgttatata
    20641 agccatttgg atttgcaaaa agtatggatt cgagttcttt taagagccaa aattccattg
    20701 atttatactc aaggatttca tccggctccg aaaatagcta cagcttggcc gctcccttta
    20761 ggctgggaag ggaaaaatga gttaattgat ctttggtttg attgggaaga tgattttgat
    20821 acggaagatc aaaagaagcg atttattgag acacttaatt cccaatcacc cttaggatta
    20881 tccattaatt cggcggaaga agttccggct tacagtcccg cattaacaaa attaattgaa
    20941 agcgcggatt atcggattaa aacggcggga agatttctcg ccgaagattt gaacaataag
    21001 attcatcatc ttttatccca aacttccgtc gaacggattc gccgtgagaa gcggtacaac
    21061 ttacgggatt taatagaatc cgcttctgtc tcggaagaaa ctgatgaggg gccggtcatt
    21121 catgtaagaa tgattgcaaa agattcagca atgggaaggc cggatgaatt tgcggatgca
    21181 ttgggacttg attccgagta tctttctttc tgcagagatc gattctatct atcgattccg
    21241 gaacattaat ccaaatcggt ggtaaaataa ttcacgctcg gcgtctatag tgaaatggat
    21301 atcacgttac cctccggagg tagaagtctg agttcgagtc tcagtagacg cgctcttttt
    21361 ttgggggagt gctggaattg gcagacaggc atgacttagg atcatgtgcc gaaaggcatg
    21421 agggttcaaa cccctccttc cctaccatcc ggataaattg ataatttttt cggacatctt
    21481 ataaagtttt cttataaaat tcctgatagg ttcttataaa tggaatagaa ttattccaac
    21541 agttttgatt attcttctta gcaaggctat gcgaaatatc tatttttaag gcgaaacaat
    21601 ggaaaaaaat actacagtca ataagaaaag ttcacgcttt acccccgttg tcaccgtaat
    21661 tattatcttc ggcatcatca gtttactcgg agatatggtt tatgaagctg ccagaggcgc
    21721 aaacagccaa tatttcaatt tattaaatat caatgccgca caagtcggac tggttttcgg
    21781 gatcggtgag tttctcggtt acatgctgcg tttgttctcg ggaatcattt cagataaaag
    21841 cggaaaatat tggctcttta tttttatcgg ttacggaatg ttaattgtga ttcctttgat
    21901 gggctttaca aagaactgga atatcctggt tgttttaatt ctgctggaga gaatcggaaa
    21961 gtctcttcgc agtccggcaa aagataccat cgtttccgga gtagcggaaa accaagtcgg
    22021 aatcgggttt gcgttcggtt tgcaggaagc cttggatcaa atcggcgcat ttctcggccc
    22081 attaattttt acacttgtct tttatttggc cggaaaagac ggaatttccg tttatcaact
    22141 cggatataaa gtgctggtaa ttcctttcat tctattaatg ttttttgtca cttttgctta
    22201 ccggaaaatc gaacgggaac atttggttcc ttctttggac gccaaagaat tccaaaacga
    22261 aaaattggga ccgactttct gggcttacac agctttcaca tttttctgca ctctgggttt
    22321 cgtaaatttc agcacagtcg ggtttcatct gaaagctaat aatttgatgt cggatgggaa
    22381 tatcactctt ctttatgcga tagcgatgat cgttgatgcc cttacagctc ttattgtcgg
    22441 cagagcttac gattctcaga aaaagaaaac cggcagaaaa acgggcggat tactggtgct
    22501 cgctttaatt ccatttatta cttttctttt gccattcctc actttgagtt attcaacaac
    22561 cttgatcatc gccggtatgg taatctttgg gatcgtcatg ggtacacacg aaacaattat
    22621 gcggtctgcg atagcggata tcgctccttt ccacaaacgt gggaccagtt atggcgtgtt
    22681 caataccggc tacggtttgg ctctgttagg cggctcttct ttgatgggct ggttctacaa
    22741 tttagataaa atcggcttga tcatcggttt gacctgtctt tccgaattta ttgcgatttt
    22801 gttttacgtc aaaatgaatc atttagtaaa agcaagtgca aaacaataag aaaatatcgg
    22861 atattttata gaacaaagcc ttgtttttgt aagcagggct ttgttcatta atagtcagcg
    22921 aaaaaaatcg gttctgcgtc tcgttaattt tgcaatatga ttaaatcaaa aatcctatga
    22981 atgtaaaagc ttctttttct tcatctgttt tgaatattat tcgaatgatc cgttgcctcg
    23041 gatctgccga aaaaatgaca tcaagtcaga tcgttgaaat acaacaaaat cggttctccc
    23101 gacaattaag acatgtactc agatattcag aattctatcg aaacttttat cgaaaagccg
    23161 gaattgacat tgaaagaccg gaactgattc aactgaaaga cttaccggtg atcgataaag
    23221 acttgatgat ggatcatttc gacgaattcg tctgcaataa acaattaaaa aaatcaaagc
    23281 tggaagattt tatcaaagac cctaattctg tcgataaaaa atataaagat gaatttaaag
    23341 tgattcatac atcggggtca tcgggaagaa tcggaatatt cgtgtacgga gcaaatgatt
    23401 gggcgattgc cgaagcagcc gtatattcaa ggatttcgaa aaacaaactc cattttatcg
    23461 gaaaagcgaa acacgctttc atcggtgtga ccgatggaca ttatgcggga atcagtctgg
    23521 cgaagtccgg acccaaatgg ttatttgatt ttttaccggt tgatatgaac cagccgctcc
    23581 atccgtcact ggttaggttg aatgattttc aacccgaatg tctcagcgga tattcttccg
    23641 gagtttatct gctcgctctc gaacagttga aagggaatct gaaaattcat ccgactcgaa
    23701 ttttatgttc ggcggatccc ttgacttccg agatgagaga aacgattcgc aacgcctttg
    23761 gcataatgcc ttataactat tacgctgcat cggaatcatt atgcatggga attcaatgtg
    23821 accgatttaa cggtattcat ttattcgatg atttgcatat ttttgaaatt attaaagaga
    23881 acggcgaaga agcaaagccc ggtgaacccg gtaatctggt tatgaccaat ttatataaca
    23941 aaacacagcc actcatccgg tacagaatga atgacaactt aattccgtca gaaaacagct
    24001 gcgaatgcgg atggccgttc cgatcggttg aatcgattgc cggccgtgaa gaagaatttt
    24061 tgttttttac agatccggcc ggtaaccggc agtttatcca tccaatagtc ttcgttgaat
    24121 tctttgcgcc ggggcttgaa aaatttcaaa tcattcagac tgaacccaat atgctgattc
    24181 tgaacgcaat tattaaagga aataaggaag aagcaattca agctatcgag aagcggatgg
    24241 acacaatcct tcaccaaaaa gaattatatg atttcgtcca ttataaaata aacgtagtga
    24301 acgatatccc caatgacccc caaacaggga aattcaggct gattattccg tatcctaatt
    24361 cttgattttg tatcatcttg tatatcgatc gaataaacct aatgtataat atcgtaggcg
    24421 gaaaatcgga ttgaaaaatc gatttgcttg acattctgaa aattcagtct gataattcgg
    24481 tcggtttgtt cagacagaga agaattcgga tcgttttgga gccgattttt ccgtcgggat
    24541 aaataattaa ttcatatata acgaggaagc catcttgaaa atcgaaaaac aaccccttga
    24601 aaatcatgaa atcaaattgg aagtcgaaat cagtccggaa gaattcgaac cattaaagca
    24661 tcaggcagca aaaaaaattg ccaaagacgc aaaaattccg ggcttccgcc ccgggaaagc
    24721 gccttatgaa attattcgac gcaattacgg tgataccgcg atagctcagg aagccctcga
    24781 tacgtttctc gaaaatcaat atgaacgtat tttgaaagaa ggagaaattg aaccgggggg
    24841 aatgggcaga ttagataaaa tagatcaaat tgatcctccg aaattcagtt tgatcgtccc
    24901 tcttgcaccg acggtagaac tgaacgacta tcgtgagatt cgcgaagaat atgtggaagc
    24961 cgctataacc gatgaagaag ttcgggaaat tttggatgag ttaaaaacga attatgccac
    25021 ggcggaaccg gcggaaggac ctgccaaaga aggccagacg gtctatgtgt taatcaaggc
    25081 tgatttgaaa gaagaagatc cggaaaccgg cgagaaagag ttaatcaaag aacgacctta
    25141 tgaattcatt atcggcaaag attccgatga aaagacagct tggccatttg ttaatttttc
    25201 tcaaaatttt atcggtgcgc aaccaaacga gatcgtaacg gttgagcata cttacggcga
    25261 agaagctccg attccgactt taaaaaacaa gacggttgtt tatacgacaa cggtacaaag
    25321 cattaaagac ctggtgatgc ctgctgaaga tcttgagttg gcgaaaaaat tcggcgatta
    25381 cgagaccttt gatgattttt tgaaagatat tcgtgaaaga ttattaaaac aaaaacaaca
    25441 aagtcaagaa aatgaatata tcgaaaaaat tgtcaaacaa ttggtggaaa aagctgatat
    25501 ccaatacgca ccgaatgcac ttgaagaaga agcacaagaa atattcagtg attttaagca
    25561 acgattggca caacagggag tcgaccttga aacctatctg aagatgaaaa ataccgatat
    25621 ggaaaaattc cttaatgaag aaatcagacc gagcgcggaa gagcaattaa agagacgttt
    25681 ggtacttcag gaatttgcgg caaaagaaaa aatcagaatc aacatggata atttcaagga
    25741 aaatgtcggt aaactccaag aaactgccgc aatagattat caaaaggcaa aaaccaacaa
    25801 ggaaaaacag gcgattgcga actatattac aaatgctgcc atgaaccaat ctttcaatga
    25861 tgcattgttt gatcgtctca tcgcaatcgc aaagggcgaa aatccggcga tcgaagaaga
    25921 agaaacaact cccgaaagtg aaaaggaaac ggctgtcgat aatctcgaaa ccgttcaaaa
    25981 tgagaccgaa tcttcgaatg atcttccggt agaaatcggc tcaaacgaga agatagagat
    26041 gccggaatct gaaacagact ccgaaaataa atcttaaaaa gtgattttgt tttctaatta
    26101 acaagattca tataaaaaag ccgtatagtt aattcaggat ttttattaaa tgaattaact
    26161 atacggagaa taaaatgagt caatcaaata atacccaaaa gaatcgaaat gataaaaatc
    26221 gatccgggca aaatcaactg aactatgtcc tcagttattt gatttttgct tttctttgta
    26281 tgtggttatt tcagcaattc atcatcaagc cgataacggt tcgtgaaacg gaaataacct
    26341 acagcgagtt taaatccaaa attaaaagca aggaagtttc cgatgtattg ctgggagaat
    26401 acagaattgt cggaatgatg aagaatccca acgcaacttc cgctgaggac gaaaaaattc
    26461 cattcacaac agttgccatg cccaacggag atccgacatt aattgaattg ttggatgaga
    26521 acggaattaa ttacacgatc agtgaacccg caaacccgct cggaagtatc attctcggtt
    26581 atttaattcc tctcctattc tttggcgtta tttggtattt aatttcacgc caagtagaga
    26641 aatcgggagg cagtttcggt ggattgggcg gaattatgag tatcggtcaa agcaaagcca
    26701 ccgaagtcaa accggaagat gtgaatgtga catttaaaga tgtcggaggt gcggatgaag
    26761 cgatcgaaga gctgcaggat attattcaat ttttgaaaga gcctgaacaa ttttcaaaat
    26821 taggcggaag aattccgaaa ggtgtgctct tagtcggccc tccggggaca ggaaaaacgc
    26881 ttttggcaaa agcaaccgcc ggggaagcac atgttccgtt ctttgaaaca agcggatcgg
    26941 aatttgtcga aatgttcgtc ggagtcggtg ccgcaagggt tcgaaatctc ttcgatcagg
    27001 cacgaaaagt cgctcccgca attatcttta tcgatgaaat cgatgcgatc ggccaatctc
    27061 gagtcggccc cggaagattt ggctctaatg atgaacggga acagacgttg aaccaattgt
    27121 tatccgaaat cgacgggttt aaatccgata aagggaaacc ggtgattatt atggcagcca
    27181 caaaccgtcc ggagattctg gatccggctc tcttacgtgc cggccgtttt gatcggcagg
    27241 ttacggtttc tataccggat ctaatcggac ggaaacaaat tttgcagatt cacagcaaaa
    27301 atattaaatt ggatcccagt ttcgacttgg atcgtgcagc tcgaatgaca cccggcttta
    27361 gcggggcaga tttggcaaat gtaatgaatg aagctgcatt gctggctgct cgccgaaatg
    27421 ggaattccgt taatatggat gatttcgagt tggcaatcga aagaattgtt gccggacttg
    27481 agaaaaagac ccgcgtgatg aatgagaagg aaagaactgc cgtcgcatat catgaaagcg
    27541 ggcatgctct gatcggtgaa attgtcccca acggcgatcc ggttgcaaaa gtaagcattg
    27601 ttccccgagg aagaggggca cttggttata ccatgcaaat gccgacggaa gatcgatatc
    27661 ttatgactca aagtgaattg gaagataaaa tagcggtctt gttgggcgga agagcagccg
    27721 agtcgattat tttcaatgaa atcagcaccg gtgccgctga tgatattaag aaagctacgg
    27781 aacttgcgcg acgaatggtt acggaattcg gtatgagtga aaaattgggc gtggtaagat
    27841 atgccacccc gcaatatcag ttccttgaaa gttatgaaac ggcagccgta gcaagtccgg
    27901 aaactctaaa actgatcgat gaagaagttc agcgtatttt ggatgagcaa ttcaagagag
    27961 tgattaatct tctcaatgaa aaccgagatg cgttggaaag tttgacaaag ttattgctcg
    28021 aaaaagagac catatcgggt gaagaagtca aagaagcaat cgctaaacag agaataaagg
    28081 aaccggcaat cgtttaattc gaattgatac ggattgagtg ctgcacttgg aaacaaaagg
    28141 gtgcagcact tttaaatatt tctaacaatt ttctatcaaa tcttatcggt gtctcatgtt
    28201 attatatatc catcaaatta acgattcgtc ggatgaaaga aattgattcc gattggacag
    28261 gagaaaaaat aaatatggat aaaccaagaa acgctgtagt gccaatggtt gttgaaggtg
    28321 gagcttatgg tgaacgtgct tatgacattt attctctgtt gttgaaagag agaattgttt
    28381 ttctcggaac acagatcgac gatcagattg caaatctgac catagcacag ctcctttatt
    28441 taagcaggga agaccctgat cgtgatattc agttctatat caattcaccc ggtggatcgg
    28501 tctacgccgg ccttgcgatt tatgatacga tgcagatgat cccaaatcgg atcaataccg
    28561 tagcttgcgg agtgaccgca tcttttggaa cagtgctctt aacagccggt tcgaaaggtc
    28621 atcgttttgc gctgccgaat tcaacgatcc atatgcatca gccattaggt ggtgtcgaag
    28681 gtcaggcttc cgatatcgaa atccaagcca aagaaattct cagactgaaa aaagaattga
    28741 atgagattat ggcaaaacat accggtcaga cgtatgaaac aatccttcac gataccgatc
    28801 gagaccgata ttttacaccg gaagaagcag tcgcatacgg gttgatcgat aaagttctcg
    28861 aaccgacaaa gtaatatttc tgatcttcat aaaaaacctc cgacatatat cggaggtttt
    28921 ttcaatcttt atgaaatcat ttcttaagag acaacagcat tttacttatc atcgactata
    28981 ttatggtcct gatcaataag cggtaaagaa attgatgcag ccgtttctta taaggggaaa
    29041 gataagaatt tattcagtat tataattatt ttaaatcaac ttaggggggt ttgatggaag
    29101 tttttgattt ttccaaaata aaatcaattt ttttggatat ggatggtgta ttatggaatg
    29161 ggaaaaatcc gatcggaaat actcgggata tttttaccaa attggatcaa gcggatattt
    29221 ttcctgtttt tataaccaac aattccacac gaacgcctga atcttatcaa gaaaaaatgg
    29281 ccgggtttgg agtaaaaatc aaaccggacc aagtgattac accggctgtt gccgcgtctt
    29341 atttattcaa aaaaaattat tcgaaagacg ttcgaattta tgtcttcggt tcacaggcat
    29401 tgaaagatta cctcaaaagg gaaggtttta ccttatcgga tgaaaaagca gatattgtct
    29461 tagtcagtct ggatcaggag atgacttacc aaaaagtagc gactgccatg cgtctgatca
    29521 atgaaggtgc aatattttat gcgacgaatc gggatcatat tttggcatcc gaaaacggct
    29581 ggcttccggg cggtggcgta atggtcagcg cggtcgaaac atgtacgcaa aagaagccta
    29641 ttgtgatcgg gaaaccgcag ccctatatga ttgagattgc tatggaaaaa ttcgggttgg
    29701 ataaatcgga agtagtcgcg gtgggtgatc gatatgacac ggatattatc ggaggtatca
    29761 atgcgggctg tcatactgta atggttcttt ccggagttga tacaaaggaa tcagtttcaa
    29821 gttttcaaaa acaacccacg ctgatctgta aagatttgga agaattcgca gaaattttaa
    29881 taagacaaaa aaataatctt gacccaataa acagaacgga taaactatga aaacttgttt
    29941 ctttgatttg aattatgagg aacttctttc aattctgaaa gaatggcagg aaccggaata
    30001 tcgggcaaaa caactttgga accttgttta ccgaaattta aattttgttc ctcaaaactt
    30061 cagtaacatt cctaaggatc ttcgcgagaa actcgatgaa acatttgaat ttcaaccgat
    30121 taagataatc cgtaacgtat cttcttctaa tcaacaggca aataaatatt tactcggact
    30181 tacggacgga aagaaaatag aagcagtctt gatgcgttac cgcgaacgaa cgaccatatg
    30241 tatttctacg caggtaggct gcgcaatgaa ttgtgctttt tgtgcaaccg gacagatggg
    30301 ctttacaaga aacctgactt cgggagaaat tgcggggcag gtttttttct tcgttaagga
    30361 actggaaaaa gaggaccgaa aattaaccaa tcttgtgtta atgggaatgg gagagccctt
    30421 tcataactat gcgaatgtta tgaaagggat tgatttgata aatgacccgg aagggtttaa
    30481 tttcggagaa cgaagaatta ccatctcaac cgtggggatt attccgatga ttgagaaatt
    30541 tacgaaagca aaaaggcaga taaatcttgc cgtctccctc cacgcaccga caaataccat
    30601 tcgaaatcaa attattccga tcaacaaaaa atatccgatc gaagatcttc ttgcagcctg
    30661 taaggcatat acggattata ccggacgaag aatcactttt gagtatgcga tgatcagcgg
    30721 cttaaatgat tcgatcgaac aagcagtcca actcagtaaa ttgttgagag ggatgctttg
    30781 ccatgttaat cttatttcat tgaatccgac tcggaaatat gatcgaaccg gctcttcaag
    30841 ggagagcatt aataattttt attccatttt agacaaagcc ggcattcctt gttcaatcag
    30901 actcaaccgc ggagtggata tcggcgcagg atgtgggcaa ttacttgctc aagcatgata
    30961 taatctatcc gcttaataca gggaaagaat ttttttgatt gtagaggaat aatggctgaa
    31021 tttttatcaa aatggatgaa aggtcttgat aaaactcgga aagttacttt ttcaagactg
    31081 gcatccgttt taggaacgaa tgaaatcgac gaatatctgt gggatgattt ggaagccatt
    31141 ctcattcagg cagacatcgg cgtcgagact tcaaacgaaa taatagagtc cctaaaaaag
    31201 acggttttag atcaggggtt atataaatca tcggacttgg ttgcttatct taagaatgaa
    31261 ctgattcaaa ggcttcccga aagccgtgac ccgttggatg aatccgaaaa aatcaaacct
    31321 tatgtgattc ttatggtcgg ggtcaacgga tcagggaaga caaccacaac cgccaaactc
    31381 gcaaaaaaat atacggatgc cggcaaaaag gttatgatag cggccggtga tacctatcga
    31441 gcggcggcaa ttgagcaact ccaagtttgg ggcaatcgat tgaatatccc tgtcattgcc
    31501 ggcaaagaag gcggcgattc tgctgcggtg gcttttgatg cggttcaagc agcgattgcc
    31561 cggaacatgg atattctgat aatcgatact gccggacgat tgcaatcccg ttacaatttg
    31621 atggaagaac tgaagaaagt tcaccgtgta atcgggaaag cgctcccggg agcgccccat
    31681 gcggtttgga tcgtattgga tgcaacaacc gggcaaaatg cccttatcca agcgaaagct
    31741 tttaaagaag cggttaaagt taatggtgtc atcctggcta aattggataa ttccgcaaaa
    31801 ggaggaatgg catttgcaat tactcaagaa ttggatttgc caattattta tgccggatta
    31861 ggtgaaaaag cggatgattt ggagagattt aatcgcgaag attttgttaa cggaataatc
    31921 ggagattaat aagcaaagat gcaggaatct attattaaga tgaattccat caaacagaaa
    31981 ctcaatgagc ttaccgctca tttggatctg gagaccaaga aaaaacattt atcggacttg
    32041 cagaaagaat cccaaaaacc ggatctttgg gataatcaag ataaagctca aaatttgctg
    32101 aaagaaataa atgacttgca agatgaaatc tcggattggg agggattatc cgaaaaagtg
    32161 gatgacacga ttgaattatt ggaaatcggt gatacatcca tcggagaaga gcttcaaaac
    32221 gacataaact cgattgaaaa agaaatccga caaaaagaaa tccaagtctt actttccgga
    32281 aaatatgaca gcggtaatgc cattctcgcg attcatgccg gtgccggcgg taccgaagca
    32341 catgattggg ctgcgatgtt gcaacggatg tacttgcgct gggcagaaac gcaccattat
    32401 gcagcggata ttgttgattt tacccccggc gaagaagccg gtacaaaaac gatcacgatg
    32461 acaatcaccg gtaagtatgc ttacggatat ctaaaatcgg aaaaaggcac ccatcgattg
    32521 gttaggctct caccttttga cgcagctcac cgacggcata cttcttttgc catggtggaa
    32581 gtattgcccg aagcaccgga taccgaagaa ataaacatcc caccggaaga tattcgcatt
    32641 gacgtattta agtcttccgg ggcgggcggt cagaacgttc aaaagaactc aactgccgtt
    32701 cgattattgc atatcccgac cggaattatc gtgacctgtc agaatgaacg ttcacaaacc
    32761 caaaatcgcg aaaatgcgat gaaagtgttg aaatccagac tgttggaaat aaaacatcag
    32821 gaacatgaaa aagaactcgg tgaattacga ggtgattacg tcaaggcgga atggggaagt
    32881 caaattcggt cttatgttct ccacccctac caaatggtaa aagatcatcg gaccgattat
    32941 gaagtcggaa atgcccaggc tgtcttagac ggagatttgg atggcttcat ggaagcttat
    33001 ctcaactata agaaataatg tgagttatta ctaaaacatc tcacatatct aaagcgtcaa
    33061 aatacattta aaggatcaat cctatgaaga agacgttttc cgaattctat gaatttattg
    33121 ccaaaggcaa tgtaatcggt ctggctatcg gcgtgatcat gggcacagcg ttcagcgcca
    33181 tcgttaattc attaattaat gatgttctga tgccctttat cggtttgctg acttccgggg
    33241 tcgattttaa aagctggttc ataccgctga atggacaatc gtatgcttcg cttaaggagg
    33301 cgcaggaagc agctgctcct gtgatcacaa tcggaaattt catcgcggcg gttattaatt
    33361 ttctgattat tgctgtcatt cttttcttta tcatgaaagc aatggctaaa gcgatgcata
    33421 ccgagaaaaa ggaagagccc gcccctgtta tcacggaacc ttcggaagaa atcaaactgc
    33481 tcacccaaat cagagacgca ctggtaaaga ataacggata aatctttttg ctttcccctt
    33541 tcgtcttccg gattttttca attattccct cagttaatcc gatccctgac cgttgattac
    33601 gataatcaat ctcacagaaa aacaggatga aaatcataaa cgataaaatc agattttaaa
    33661 ggtatactct ttataaagaa gtaaatattt tgatataaag tattcatcta aatcaaataa
    33721 tttcttctgt gtttttcggg attatcagtt caaacaacat ttaacattct accaggagga
    33781 aaaacaatga aacgtagtct aatctttact tttgctctgc tcatattggc aattgccgca
    33841 ccggttttcg cccagagtgg tgaattaaat gtcctttgca caccgcaaga gttatggtgc
    33901 caagggatgg aggaagcctt cgaagcaaaa tatccggaga tcgatgttca gtggatccgc
    33961 ttatcatccg gtgaaggttt agccagagtt cagtctgaag ccggtaatcc taccttcgat
    34021 atttggtggg gtggcccaat ggatactttt attgcagcca aacctctggg gttgcttgag
    34081 caatatgaat ctcccaattt gaaaaacatt attcccgaat attatgatct ttttgttgac
    34141 aaagaccatt attacaccgg gatctatatg ggttcactcg gtttcattac caatacggat
    34201 tggcttgccg agaatccggg tgtggaagca ccaacttctt ttgaagattt gctgaaaccg
    34261 gaatttaaag gtcagattgt tatttcgcat ccttccactg cgggaaccgc atataccttc
    34321 ctttcaacgg ttttgcaggt gatgggcgaa gaaaaaggtt gggaatatct taagaaattt
    34381 aatgaacagg tagcgattta tacaaagtca ggttccggag ccggaaaata tgttgcgaat
    34441 ggcgaatttg ctgttgctat tatgttctca catgacggtg ttaaagaaat tgaagacaac
    34501 ggcgctcatc tcgtgattac tttcccgaaa gaaggaacgg gatatgaagt gggtgcggaa
    34561 gccattctta aaggtgcgaa gaaccttgag aatgcgaaac tttggtatga ttgggcactg
    34621 actcccgaag cgcagtcttt ggggccaaaa ttccaatcct atcaagcacc gactgttacc
    34681 ggtgtcgaac tttcccatcc ggaattactg caggtaaaac tgatcgatta tgattttcaa
    34741 tggtctggcg cacataagca ggaatttcaa gacaaatttg ccaatgaaat tcaaaatgcc
    34801 gatatggatg aaagcggcgt attaattaag caaccatagt tccttgatta agatttttgt
    34861 cataaaaatg ggcggaagaa aaccgcccat ttttgtaaca tttttttatt ctttaatatt
    34921 tataaatttc tcaatcggag ttgagtatga ccgtaaattc tgatagtatt cccccaaaaa
    34981 ccagatttca agggatcaag cgtaggattc atgaattcgg attaattgcc caagatccga
    35041 tattgatgat tgctctgatt ttttcgatca tcattttgac tatatttatt ttctttccta
    35101 tttttaggac gattataaga ggatttgttt ccgataccgg cgaagcagat tttactcatt
    35161 ttgccagata tttagacgga acttttttac agggaataaa tactttcttt aaaaatgcgg
    35221 gcttgatttt gcaggaaacg gcaacttacg gacgaacata tcgcttggtt ctttccaata
    35281 cgattaagat gggcatcttc tcagccactt tcggaacatt tctcggattc atttttgctt
    35341 atgccaccgt gagatgcaat atcccgggga aaaaatttgt tcatctattg gcattggtgc
    35401 cgacggtttc tcctcctttt gcggttgcaa ttgccatgat ccaaatgttt ggtagaaacg
    35461 gcttgattac aaagacttta ttgggaatca aattcactcc gggcatgaat gatatttatg
    35521 gaatggacgg tctggttatt gtacagacac tgacattttt cccgatagcc tatttaatgt
    35581 tggaatcgat gatggaaaga ttagacgctt cgatggaaga agccgctgaa acattagggg
    35641 ccagccgttt ctatcttttc agaacaataa ctctcccgct tttgattccg ggaatagcat
    35701 ccagcttttt attgcttttt gttgaatccc ttgcggatct cggcaaccct caatttattt
    35761 cgggaaatgt cacggttttg tcttcgcaaa tctatatgtc ggttatcggg gaatttaatt
    35821 atcaaaaagc ttccgctctc tcttttgtac tgttgcttcc caccttaatt gtttatatcg
    35881 ttcaaagata ttacataacc cgtcgttctt atgtttcggt aaccggaaag cccaccgttg
    35941 gcagggtcta tgaaaaaaat aagacgattc gcattatctt cgcaaccatg gtttaccttg
    36001 tttgtattct tatcttactt gtttatatca ccgtaattgc aagttcattc gtaaaaggtt
    36061 ggggaatcga ttttactccg acactttcaa attggacaaa aatgtttgga cgtggcttgg
    36121 aagccatttt cgataccacc tttctcagcc tgtacgcgac accctttgca gctttattgg
    36181 gaatgattat cgctttcttg attgttcgga aaaaatttgc cggcaaagaa attttggatt
    36241 tcggctcgaa tctcggcggt gccgtccccg gcacaattgt cggtatggga tttattttgg
    36301 cttttaactt taataatccg gggttagcag ttttcttgat catcgtttgt tcatggtttt
    36361 atatcacttg tgtatcgaaa gccaactttc aacgggtaaa aattctcatt ccggggatta
    36421 ttttgggatt aatcatgagc tgtttggatg atggcggatt tttatgggga aaaaatgggt
    36481 ggcttaccgg accggtttcc gccgaatcac aagctcccgc cgcatttggt atcggattaa
    36541 acggaattta ttatttgata ggtgccatct tcattttgat cggattctac tttctgcttc
    36601 agaaaaatta tcgtaaatta ggaaaatatt ccttttttat cggtatttat attatcctaa
    36661 ataaagtgat cttgatcatt tcaaaaccta tccgaatata cggaaagaca caaagtgtcc
    36721 gctggtggaa agcagtttgt acgcagtttg cggattacat tgaagttcct ttttctcttc
    36781 cgactttctt cctcggcgtc tgtttgtttt tcattactgc aatcatgatc tatgaatacc
    36841 atggaaaatt tgaaactttt ttcaaaaccc ttttcatgat tctatcaatt gccattttat
    36901 tcaccggtga tcaaatgaaa atgaatggta ctgcgtatat tatattattt gcctttctaa
    36961 ttcgatctct ccccgcatcg gtccgttccg gtgttgcggc actgcaacaa attgacggat
    37021 ccatcgaaga agcatccaat attctcggcg gagacagtca atacactttt cggaaagtga
    37081 ctttgccgct tatcttgccc gcctttatga caggtttgat cttctcattc acccgacaca
    37141 tgacaagcct ctcggcaatt attttccttg taagcgcaaa atggcagatt gttacggcat
    37201 ccattatgag cggatgggag caagatggaa tcagctatgc ggcaacctat tcatccgtta
    37261 taatttttat cgttttgata tttatcggat tgatgggcgt tgtaacccga aaagttttga
    37321 aaatagatac caacattgat ctgaacgcag gcttctgaat agggagagaa gattatgtac
    37381 ttagtaattg ataacgtagt taaagtattt cctgcaaggg gtggtgcgaa agaagtcagg
    37441 gcacttgacg ggattacaat cgagattaat aaaggagaac tggtcacatt attggggcct
    37501 tccggttgcg gtaagacgac cacgctccgt atggtcgcgg gttttgaatt tccgacttcc
    37561 ggaaatatta ttctcgacgg gaaaattatt aatgatgtcc cgacccataa gcgagatatg
    37621 tcaatggtct tccaaagtta cgcaatattt cctcatctga atgtttatga gaatattgct
    37681 tacggattga atgttcaaaa aatgagcaaa gaggaacaga aaaaaagaat cgatgcggtt
    37741 ctcgaaatgg ttcacttgga aggatatgaa acccgtgcac cgaatcagct atccggagga
    37801 caacaacaaa gagtggcatt agctcgggcg ttagttatgg agcctaaagt ccttctcatg
    37861 gatgaacctt tgtccaactt ggatacaaaa cttcgtgaga aaatgcgttt tgaaattcga
    37921 cagttacaac ggcaacttgg tttcacttgt ctttatgtta ctcatgacca attggaagca
    37981 atggtgcttt cggataaagt tgtcgtgatg aacaacggcc gaattgagca gatcggcaaa
    38041 ccgcgtgata tctatcgcta tcccaagaca aaattcgttg ccgatttcat cggtcgggca
    38101 aacttcttgg aaggaaaaat cattgaaaaa agaaatgatg aattgattat tatggttgaa
    38161 ggaaacccgc tgacgattaa cgatattcat tataatgaat ttacgatcgg ggaagaagtg
    38221 gaaataatcg ttcgtccgga aatgattcaa gttcgcacag aggaaggaaa gtattcggca
    38281 actattaaac aagccgcata tttgggggaa tcaattgaat atatccttga ttttaatgga
    38341 aacgaattaa ccgctcatga gaccgacccc tcacgacttg aaataatacc ggaaggttct
    38401 tcggtaaaaa tttctttcat ggaagattgt ttacatatcc taaaaaaata aaggtatttt
    38461 tttgaatttt caagacggat taaggcacta agaatgcata acccgtcttt gttctttaat
    38521 ttgctctttt tcgatcctat tcaataatcc gtttttttac gatcgttttc tctaaaatgg
    38581 ttatgaatcc taatttcgaa tataatgggt acattgaaaa aaatggaacg gatattatga
    38641 atcgaaaact gatcgtcatt ctttttgtct tgttacttcc ggcttgtaca aatatgccac
    38701 aggacatgaa agaatctatt tacaccgaag ctgcccaaac tttgatcatt caattgactg
    38761 aaacggcagc tgccgttaca cctagtgaaa cagctacggt cattccatca tcgactccga
    38821 ccgaagaacc gactgtgacg acggcaccga ttgtcattcc gacaatcgct tcgaatgtta
    38881 ccgttgctcc gacaacctcc acagcggata atccttataa agccgaattc gtttgggtcg
    38941 atccttatcc caaccaattc atcccggggc agaaatttac attgacatgg caattaaaaa
    39001 acgttgggac ggcgacttgg tcaggaaaat atcattttta tcacaataaa ggaattcagc
    39061 ttgcggatca atcatcctat tctattgata cggtggtcgc tccgggtgaa atcctgacca
    39121 tttccatgcc tgcgaccgca ccaagcactt tcggaacata tcaaacggaa tgggcttttg
    39181 cgaatcccga aggggttcag ttttattacg tttattacac cgctatcgtc ggagaacaaa
    39241 cctttgttac tgatcaaccg ggcgcaccga caagtactcc gaactcactt tactggatgt
    39301 gttccgaccc ggatcgaagt aatattcagg gaaacggttg cgaagaatat tgtcacctga
    39361 acggcagaag tatggatcaa gatggggtta agtgttattc atttggaaat ttgataacag
    39421 atttttatta ggaacaaaca ttgagaacca tatacgatat taagagacac tcatttttat
    39481 aaaaagtctc cgggagattg gaagcaatat gttaaaaaga aagagcttgt tgatcaccgt
    39541 tttgtgtacg atgctcgctt ttacagcttg ctcaaacaca ccgaataaag tagttcagct
    39601 caatacggaa gcggcagcca ccgcagctga aatcattacc cagaccgcag ccgcaattac
    39661 tccgaccgat acagccgtcc cgcctaccga aacaccgaat tataccgcaa cgccgatcat
    39721 tcttccgact atcctgcgtt atgcggagac tgtggcaatt gcaacagttg tttccggcca
    39781 accggctacg ggtctgtaca atggaggaga actaatcggt ttttcaccgg caagtaatat
    39841 acaggttacg ccgaaacagg cattttattt atccgttcag attaagaaca ccggaacaac
    39901 ttattggaat acgtcctaca aaatcatcca ttcgggaggt actcagatgt cgtatgcgac
    39961 ttcttatccg ctgaccggag atgtcgcttc cggtggaact tacaccgcat cgatatacat
    40021 gactgctccc gaaaattatg gaacttacac acagacctgg acactggtag atgcatataa
    40081 tgctactgtt ttgacttttt cctataatgt aattgtcgga acgacatccg tcataacatc
    40141 tgttcctacc ttaactccga caatcacatt gacaagccag gctaccgcct attcatccca
    40201 acttgattat atgtgttcgg atgccgctcg aagcatcgaa caaggtcagg gttgcgaaga
    40261 ttattgtaaa attgccaatc catacaaaag cccttgctat tatcgcggaa cgatcaatcc
    40321 gaccgctaca aatgttcctt cagcgacatt gcagccgacc aagacctata cgaccgcccc
    40381 gactgcagct cccacatcta ctccgattcc aacctctaca accgttccac cgacggatac
    40441 tgctgttccg actgaagaaa cgccatcaca acaagatggt tcggcagacc aaaatactta
    40501 atagttaata ttgaacgttt tttggctgaa tattaacaaa ggtaagaatc ggaattttgg
    40561 gataacagga atgaaattat caaaaatacc gctttt
//
LOCUS       DAOSMM010000014        39336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1066_length_39336_cov_18.457804, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000014 DAOSMM010000000
VERSION     DAOSMM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 39336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39336
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1066_length_39336_cov_18.457804"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK898_03735"
     CDS             <1..435
                     /locus_tag="PK898_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU19176.1"
                     /translation="PAQRGLYFVWNDPAVSVVLSGMGALEQVKENIAAAENAAPGILS
                     EKELALYSRVRDMYWKRIIAPCTGCEYCMPCPHGVNIPENLNVLNELYMFDNFAESKR
                     AYEFLPKEARAEQCINCGTCLRKCPQKIQIPVHMRALADRMA"
     gene            complement(501..3227)
                     /locus_tag="PK898_03740"
     CDS             complement(501..3227)
                     /locus_tag="PK898_03740"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOU19177.1"
                     /translation="MDGTNINQHELPKTYDFAANEEKLYRWWEEQGYFKPWNDPNKPD
                     FNPDHTPYVISIPPPNVTGELHLGHAMFVSMEDLMIRYHRMKGMPTLWVPGTDHAGIA
                     TQMVVERALNAQGIKREDLGREKFIEKVWEWKEKYHDRIVAQIRRLGASCDWDRERFT
                     LDEGLSKAVREVFVRFYEDGLMYRGPRIVNWSPKLQTAVSDLEVEYSEEPGTLYYFKY
                     MLADGSGDYLPVATTRPETILGDTAVAVHPEDERYQKYIGKTALVPIVGREIPIIADE
                     YVDREFGTGVVKITPAHDPNDYKIGETHHLEMINILTKDAKINEAGAPYTGMDRFECR
                     KKIWADMKEAGLVIREEPYMMNIPRSQRSGEIIEPMISTQWFLKMKPLAEPALEAVRN
                     GSITIIPDRFTKVYYNWLENIQDWCVSRQLWWGHRIPVWYCQDCGEVSASRTDLEVCP
                     KCGSKNIEQDPDVLDTWFSSWLWPFSTLGWPEETPDFKYFYPTTMMETGYDILFFWVA
                     RMIMSGLYFTGKIPFSQVYLHGLVRDEHGMKMSKTKGNVVDPLTVMDQFGTDALRFTL
                     LVGSTAGNDTNLSVKKVEANRNFANKLWNAGRFVIGALDKAPSRADDGSPIQPTLADR
                     WIWARLQNLITDVERLFDSHQYGEAGRQIYDFFWSEYADWYLEIAKIQLSRGGNTAFN
                     TARLLVRILDTCLRLLHPFTPYVTEALWGHLKTAAIDQSEAYAPAHGWEEALIIAKWP
                     EAESTGESDQLAVKDMLMIQDVIRAVRNIRTEKNVKPGTLIPATIVTGEKTAVFESQR
                     DAIATLAHLDPDQLEILPHLDTPPADRISTAASGIGIFLEIEEEIDLAAERDRLQKEL
                     AETDEQIDRLEKLLNSPFSMKAPAAVVEKEREKLAKYRETAINLNKQLSGLR"
     gene            complement(3657..4064)
                     /locus_tag="PK898_03745"
     CDS             complement(3657..4064)
                     /locus_tag="PK898_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19178.1"
                     /translation="MIWIVPLIFVLVAGYIFWWNSNENKAIPDTDAVDLFKEAQRILS
                     NSVFKRNSLFSRPTAQFGYPINAGHPNGQPPAQTNETPKEIETPGDSAQSGSTDHSSG
                     IEKLPSSSDSEEPVSHEDKSEDQNKDNSSHPDS"
     gene            complement(4061..5227)
                     /locus_tag="PK898_03750"
     CDS             complement(4061..5227)
                     /locus_tag="PK898_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOU19179.1"
                     /translation="MNITIDTMNVTLADTPKTLAKVAAWLEKCSEVAVDTESNSLYVY
                     YEQVCLLQASSEENHAILDTLALKDLSSIAPIFSSKSILKVFHASEYDIMCLKRDFGF
                     EFNHIFDTMIAARILGRKEVGLAAMLESELGIRLEKKYQKANWGIRPIPEDMLKYAIH
                     DTDYLIELKDKLEQELSERNLTELAQEDFDRVCKTPSGQYAPETTNWWNIAGNNHQLT
                     VQQIGMLQAVCEWRERIAKNMNIPVFKVMQNHVMLELCLNPPQSEEELANIKGMRKSL
                     IEKEGKNLMKIFKTTDRDIRCIPRPPKTKIPCDSTLQRREHLRNWRKETGIKYDVPSD
                     VILPRDIMEEIICKNPQNEAQLQSIMVDLPWRYRQFSTEIIQLLNKLEEQKEFA"
     gene            5394..6425
                     /locus_tag="PK898_03755"
     CDS             5394..6425
                     /locus_tag="PK898_03755"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU19180.1"
                     /translation="MEKTRTKKLFIFFNPAAGHEKKVDLFENRIREWGKNNGWRIDIH
                     RTHKNEDLKKALRQRQKDGIDVIGVAGGDGTVSEVISAMAGIDLPLLIIPAGTGNIFA
                     RNFYIPFNLEKCLSLLNEGSEVGFVDLMLVNGSYRMLNAGAGFNARLMISTTRDEKQK
                     LGFWAYLKNIGKSLKRTHQVEFVLTIDGKEERIVGADVFIPNAGFGMHSWIEENLLNP
                     NDGVISAFVTRPSDVQSFFRILWDAIKGKSRGVEVISRFEFKDELLVESDRPLPAQSD
                     GEPIGTTPLRIKVVPSSLKLIIPVRESPLAGFESINKYFSRILNLPILEEQTSLKIPK
                     RIFSDQNRK"
     gene            6497..7621
                     /locus_tag="PK898_03760"
     CDS             6497..7621
                     /locus_tag="PK898_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19181.1"
                     /translation="MAGKVLRSKKGILLLLLLSVFIGSCNTAKSDDALEQEVASFVKT
                     LQMPTGDPWLAAIQPTLLPSDTASPSPILLLNNQTNSAPTETVFIPTDEPTAIPVPPT
                     EVLSAPIDTSNSPTETPLPFYRPVIVDPNPSGADWKSLPIIPESITETAKDIYRYGVS
                     KMGRRPNYFSKIGDCQSYPNVFMGEYDYVSGSYELSDDDLYLEDAINYFQESFGALSF
                     AVANGLSAASLLTTIWSDPSYCESGESALLCELRINKPSIVFVNLGTNWVSTADVSLY
                     EDYLDEIVYTIIQYGTLPILSSKADNVEGNNQINEITAAIAKKYGVPFFNFWAAAQSL
                     ENHGLDPDRSDIYLSVDAWKVRSYWGLKMLYTVGHSLELF"
     gene            7668..8060
                     /locus_tag="PK898_03765"
     CDS             7668..8060
                     /locus_tag="PK898_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19182.1"
                     /translation="MKKIIYSLAMIILCVQLSGCGGNKKVSEESKVVESFYNAIVNQE
                     RDKIGSIVCSDWEKDGLREVDAFMGVKSELKDFSCSVQKEEADNAEVTCTGSIAASYG
                     NEITEFPLKDRIHKVVKENGEWRLCGFE"
     gene            8184..9611
                     /locus_tag="PK898_03770"
     CDS             8184..9611
                     /locus_tag="PK898_03770"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOU19183.1"
                     /translation="MLLSNILLLAGLFLLIRGIPQQRIRKYFLLALSIIFVFSLQPTL
                     SIRNFDFWLPLTTTGLTLVCWAFCCRNDTSRLSDNRSELLIIFGTVLFIALSRLFSFE
                     GIFTASRPPILSVSLPWIAGWFILFFIFYYFGGSKPKKLFSLFIGFILLILILLKTPR
                     LALQVSRFFRNLAGQSAELATANDLRWFGFSYIAFRLLSVLIDARKGRKWVATPGDFL
                     IYVCFPPALAAGPIDRLDHFDKELNSVPHPFPEDFREGIPRIATGLFKKFILADSLAL
                     VALSSQNAVLIKHTGWAWASLFAYSLQLYFDFSGYSDVAIGLAHLIGIKLPENFNHPY
                     LKPDIAKFWNSWHITLTQWIRSYTFNPLVRKLRKKKDPAFPQWFMILITQSVTMGLIG
                     LWHGVTLNFFIWGIWNALGLFFHQLYADHTKNQLTVISEEHSIAFRIYTIGSTLLTFL
                     FVSLGWIWFVLPNIDSAALFFERLF"
     gene            9619..10722
                     /locus_tag="PK898_03775"
     CDS             9619..10722
                     /locus_tag="PK898_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19184.1"
                     /translation="MKKILSFTLKVFFFLIILLALYYFIQPEPFLGKISLYNFIFPGR
                     ERLPFGENPRVAYNFSLYNLNAMFASHEISGVQEQDNSFRVITIGDSSVWGTLLKPEE
                     TLAGQLDGKKIKIHGAERKLEVYNLGYPTLSLAKDVLILQRGMAYKPDLILWFTTMES
                     FPNEKQIESPLLAKNSEEFAQLLTSYGEIPSWMPKSSPESFWEKTLFSQRRALMDLIR
                     LQLYGFMWAATGIDQDYPADYPVAQVDFDRDNSFHGVDGIYPNDQMAWELLNVGMRIA
                     GNVPVILINEPILISDGENSDIRYNFYYPRQAYDVWRKDLADRCTEEKWKCLDLWNLL
                     SQSDFTNSAIHYNSEGGQKVVDTILDVLPESLQ"
     gene            10732..11601
                     /locus_tag="PK898_03780"
     CDS             10732..11601
                     /locus_tag="PK898_03780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOU19185.1"
                     /translation="MKKPPSCSKNLFFLILSLPFFFSVPSFAQSIEDRRPSDFGRTWQ
                     DLPIVPELNENSLRILKAGIAAGHNPHAFSKVGDCDTSTDWYLADFDKDSRYYNLGEY
                     RDRFEPVLSYYKGSFSHRSLAAKPGFSAASVLSNYWLDFNSCEPDELPLTCEYRIQNP
                     LIVLISLGTNDGYNPPAFKENLRTIIEITLSENRLPILMTKADNVEGDFSINQDIADL
                     AQEYDLPLWNFWAAIQDLPNHGLVEDGIHLTFYKNDFADPNAFNYAWTYRNLTALQLL
                     EKLMQATEKLQNK"
     gene            11611..11856
                     /locus_tag="PK898_03785"
     CDS             11611..11856
                     /locus_tag="PK898_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOU19186.1"
                     /translation="MIEIVDILIDKIKNEILNQPNRKLKSDTPLISSGLIDSFHLVDL
                     ALMIEDTFGVHIEDYELRSDIFDTVDDLALLIDSRMS"
     gene            complement(11883..12392)
                     /locus_tag="PK898_03790"
     CDS             complement(11883..12392)
                     /locus_tag="PK898_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU19187.1"
                     /translation="MGDYHSRLHQLAGSFPLVEFFALTYITDPQGNLLLIRKNENSCW
                     ELPGGAMQPDESVFECLQRTVLSVTNVFIEKGMLIQLFSDGELNYIDEDSANITPVYA
                     VFIPTSVHGSLRISREKPVEFRFFASWNIPTDKIFPPMLGAIQYYRQHFPEGIPLQPL
                     DLDQFAGYG"
     gene            complement(12394..13176)
                     /locus_tag="PK898_03795"
                     /pseudo
     CDS             complement(12394..13176)
                     /locus_tag="PK898_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(13678..13836)
                     /locus_tag="PK898_03800"
                     /pseudo
     CDS             complement(13678..13836)
                     /locus_tag="PK898_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            complement(13931..15892)
                     /gene="dnaG"
                     /locus_tag="PK898_03805"
     CDS             complement(13931..15892)
                     /gene="dnaG"
                     /locus_tag="PK898_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOU19188.1"
                     /translation="MSQIDEIKNQIDIVDLVSETVKLRRTGKNYIGFCPFHPNTRTPS
                     FVVFPDSGSWRCFGQCAEGGDIFSFLMKRDNLDFQQALQVLANRAGIKLETFKQEDKT
                     KKEQINNLHQLMQNAIDYYHNNLIHNPIGKPALDYLHKRGLTDETIHNFEMGYAPDSW
                     DALLQHLLSMNAGRQDLIDVGLVSEQRDDKGEIIKDGKIFDRFRNRIMIPIHDSSGNP
                     IGFGARILDPNDVPKFLNSPQTVLFDKGKTLFGLNRAKSSIREKNQAVIVEGYLDVII
                     LHQAGFTNTVSPMGTALSTDQAKLLARQSKNMILALDADAAGDVATIRGIDVIRSAVK
                     NDKEETVESQTLIRQERKLNADIRITRIPEGMDPDEVVLRDPSEWQQILDSAKPVVIF
                     MMETLANGRNLEDAKTKSEIAKQIMPLIREVVDPIERETYRQQLARFLKIDENLLNYI
                     SPQKSKKSTQWEIPDRLDFQSKPSLVFDPKEGIYSKEMTILQFLYHFYNTPGAISKID
                     RNLRKYNLGPLRKEDFEESDLRVIAGCYFDGLNQDDELNTQKYIIENIPDSIRPTFEK
                     IRKPNFKGSINPVDLDIDLTRSVAYLRNEKNIFNQLELQTLYKEQLESGSNPEEYDRL
                     IDQLIHERKNLEKLLKSLDQRLTEEPNGK"
     gene            complement(16093..16872)
                     /locus_tag="PK898_03810"
     CDS             complement(16093..16872)
                     /locus_tag="PK898_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU19189.1"
                     /translation="MDFKTFGNQKNPSLLLLHGLAMTWKSFDEIVPLLMDDYFLVIPV
                     LDGHNPDNRSNFQTVQEEAEKIEKKVVDDFDRHLSGVLGFSLGGTIAIELLTRNKIQM
                     DRVILDGACASTPGSLSRPYASMWISMLSKFTDGKNISSFLKKVTGSGDVEALYNMLF
                     FNLSEKTITNACKAAFEYEISPYVGQTKAQVTFWHGQREPYPAKTAKKMKEYLPAMRI
                     RIFGNLGHGELFSRFPQRYVAEIRNFLLPENQSTDALQTES"
     gene            complement(17020..17505)
                     /locus_tag="PK898_03815"
     CDS             complement(17020..17505)
                     /locus_tag="PK898_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU19190.1"
                     /translation="MERYQVIPRTLIFIFHDEKILLLKGAPDKKNFPHLYNGLGGHVE
                     RGETILECARRELKEECGLTLLNLTLCGILTDITGEQVGIQIHLFKGSVDQLADHLHS
                     SSEGTIGWFRKDEVAHLELVPDVPFYLETVWNWKPGDPLIFGIIDRRNTPDNLIRFTA
                     A"
     gene            complement(17508..17708)
                     /locus_tag="PK898_03820"
     CDS             complement(17508..17708)
                     /locus_tag="PK898_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19191.1"
                     /translation="MKDEKQGCLSGLLQLFLVKNIYDWAQRKFGYGNGILGCGCGCLL
                     MVVFIIVVFSILTKTNFLDFSF"
     gene            complement(17806..19245)
                     /locus_tag="PK898_03825"
     CDS             complement(17806..19245)
                     /locus_tag="PK898_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19192.1"
                     /translation="MKKTIRSFALAILLSLFVGIFTTLAQTDGGVIQFGAGESSAWIS
                     GSLAPNESIRYSFYALSGQIVSISVKSANNSAIIGFSNSKDYFSYLSPDRKYMYYTMP
                     LPQTGTYYLTVQDTGPGTDFVLQLTIPPLNQVPVQPVQSGQPVYSQPKSLSGGTIQFA
                     EGQSSATISGWLDAGSDITYQFYASKNQVLSLAVTSTNGSVFAGIQDGTGYIYLSPEK
                     RWQYYISPLYSTGPYYLTLFNPGGGTNFSFQITIPPLNQTGPSVPILPTTQPVPVMPY
                     PISQNGGAIQFAPGETSAWISGNVSANNCIRYSFFGGNNYKMIATLNSATGSVSLGVS
                     DIYGTVYLNSFNRSNYWMMPLKQTATYFLDVCNYGQQPADFTLNLVIPVNIPTSYAVK
                     TGTLRSNSVVSYTIFGHADQVMTVNLNSGATPQAYLRIMGLGDGVVYLDNNAFQTYWS
                     GTLPGDQDYLIDVVAFGAASNYQLSVSLE"
     gene            complement(19305..20750)
                     /locus_tag="PK898_03830"
     CDS             complement(19305..20750)
                     /locus_tag="PK898_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19193.1"
                     /translation="MKKNGIICFSCLLLLFELSGCSLFTPKAAILSEKNRKTDQTEIP
                     IGTNTVQIILKPTENIIQTPFVTADFQSAPEKAAPENPTLLPGIEPSPSVTQTKTAEN
                     HSSPQYSFTSTPIAIPDLTVTLQPTAVPLRLIATAAPLKVQRDHSKAFLITLSIPSVT
                     LSPTYTRTLEMTRTSIPTSSPIPATATKLFSTATKVTEPTETSTATRTASATLQKTIA
                     LFLSPTAAATETAIPTAMKGFVKPTETPIPVIFITPIIVCENNGTCVKNTVTPTQISN
                     FELIHFSEFDNSVVVSGEIKSESEKTYTFIANKDQVLRIGLASSSLSKPDLILQNSIG
                     TNIEPQQSANEMFFNLNENGWYNLKITAPDHDLSYNLELSLPERLSFSKNQSVIHLSD
                     RINASSTRRFTIEIPAGKYLKLSLKILNGEPVDFHIYDLSSGKENSLNVNRALAWQSG
                     KDGHKFLIEMKSVSVSVDFELTLESVQPENL"
     gene            complement(20779..22263)
                     /locus_tag="PK898_03835"
     CDS             complement(20779..22263)
                     /locus_tag="PK898_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19194.1"
                     /translation="MIRAGLQAGILLILFFMIPACQTVPASEKTKTLGSSAEPAQSTA
                     SIIPTLFFTPSVQPTASPTPLPQHVNLGLEDVAGMTIRVWHAQQNEVKSKLEELVDRF
                     NSEDEYGFTVSLHNELSDSILNDVLMTPPPANEFPNIVIAGSPWLKSWQAAKFPMVML
                     QTYIDHPTYGFEAQEIPPVMDSMMSQEYYRGNLTALPLWHLPEFLFYNDTFGQILGFS
                     TPPANRDDFTEQICAALAAVNSDSDPGNDGTGGWILTSSPGAVISWMMTTAPQKADFS
                     NFPEEKDRDLFTDTVTWLRELFDQGCVWQSRLSEPFDYFANHNALLYSGTFADIPFQR
                     RAFENADSNRSDDWTLIPYPFNNSDNENSPVIFSTAVSAGIFTGTAEEQFASWLFLSW
                     LMKPENLTELALAANAWPVQDTPEITKMVKEKADSKIYASLKWRTAIQPSPLPADWNT
                     DSLVLSDGFAYAFSPGMNITKIPEIWDQIESTLTEIKQVDQQKL"
     gene            complement(22306..22956)
                     /locus_tag="PK898_03840"
     CDS             complement(22306..22956)
                     /locus_tag="PK898_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU19195.1"
                     /translation="MNNPSYQYEDLTMIPFQSGPLLNNTYLIADRKSKECIAIDPSFF
                     FEQVLEKIDREQWNFKAIWLTHAHFDHIAGIRSAVKSYPQIPIAMHPDDEIIRENGGI
                     ASNSSAGQEKYCPKPAIHLRDGMTLAVGKYEFQVLHTPGHAPGHCCFYQKEAGWLFTG
                     DLIFYHDCGRTDLLGGNPAALMNSIREKILTLPDETLIFPGHEEFSSVGAEKPYYK"
     gene            complement(22953..24056)
                     /locus_tag="PK898_03845"
     CDS             complement(22953..24056)
                     /locus_tag="PK898_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOU19196.1"
                     /translation="MTKTNLEKLKNLMNEHRFDAVALIPGSDFRWLTGTEKHLMERPT
                     MILVTVDKDPAIVAAGFEMNSFDRIGFNFRAFPFSDNPSEWGNAFRQAGEYLDLAGKT
                     IAVDPLHFRFFETKLLENAVTDCQIVSGESLFKKFRLQKTEEEISFMKKAAIIAETAL
                     EKTLKIVRPGISEREIASELNAQLMRAGSDSTLPFPPIVAGGPNAADPHAAVSDRILE
                     SGDFLLFDWGARYQGYCSDITRTFAVGDISEVQHEVYNTVLAANRAAKRAARPGIPCG
                     AIDRAARDEIEKAGYGSFFTHRLGHGLGMEAHEDPYIYAENSLPLSEGMVFTDEPGIY
                     LPGKFGVRIEDDLVITNEGCEQITSFPRELRIL"
     gene            complement(24135..26183)
                     /locus_tag="PK898_03850"
     CDS             complement(24135..26183)
                     /locus_tag="PK898_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19197.1"
                     /translation="MAEKSKSLRHGNLLICLILVAITLLVYLPGMNRLGYYRDDWNNV
                     FNAYTQGSQMLIRHYQSDRPVDGYLLSAAYDLFGPNPYPYLLINLACRFFCGFFFYLT
                     LTLIWKKNKFPAFVAASLFLVFPGFLRQVDGIAYLPHQTAMLAGMLSVYLTILSLELR
                     KSRLKIPAVLLSIILAILEMFLMEYYIGLEALRILLIGAYFYNSADDRFPRIVGKTIL
                     NYLPWLVGALIFFLWRSFLFEATRSGADVQDTLRPFFEHPRYYGMDWVAKILKNTFKL
                     LFGSWIIPAYNLLSQLEVKRFWKIFAYSLLPAAIFIIGFIRLMPERKEKISAESSSSK
                     NKKAGWRQQWIVIGLVSTVLAIAPMIIANREITFSSSLDRFTYHASFSAIIFLVGLIA
                     SIEGKWVKTALFAVLILAACMTQITNKENYIAQTDVVNDFWWQLSWRAPDISPGTLVL
                     LSMDGFSAEEDYELFVPLHLIYHPDEDHVVIGSDLLNQDSAKKADMKKIEDRIVREIY
                     LRKDYSQILALTKPTVKSCLQVIDGRNPIYTPAQWSRISEIGSYSQIDKILTDADPHV
                     PSSEFFGKEPDKNWCYFYEKMSLAQQSEDWEKVAEIAGEALSAGAHGEDPLEWLPLVQ
                     AYAYLGRYSDAKPYGEILKTDEYLKFETCRYFRIAAGILNDPSPAKKALPWLENEFCK
                     "
     gene            26317..27924
                     /locus_tag="PK898_03855"
     CDS             26317..27924
                     /locus_tag="PK898_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOU19198.1"
                     /translation="MSYIPEKKTESLWWRDGVIYQIYPRSFMDTNGDGIGDLKGIIGK
                     LDYLSDLGIAAIWLSPVMPSPDADFGYDVANYKDIDPKFGTMDDYKNLIAEAHRRGIR
                     IIMDLVLNHTSTEHPWFLESKKSKDNPFHDYYLWQDHPNNWKSTFTQRSAWEYVESCG
                     QYYYHMFTVQQADLNWRNPEVYSEMMNIFRFWADLGTDGFRLDVFNLYFKDDQFRNNP
                     PAFHWIPFMRQKHIYDCDRPEMDRAVRDIRKVTNAYKDSYVVGETFLSSGEKIKRYCG
                     EDKLHGAFDFTFTHCKWDASAFRKIIKNVEQNSQEAGFWPTYVLNNHDVERSASRFGL
                     GEYDERLKEAAVLLILLRGTPYIYYGEEIGMRETKLKKSEMMDPVGVMWAPFNRGRDG
                     CRAPMQWNNQTNAGFTSGTPWNKVHPDYPERNVNAMVHDPNSLLTFYRQLIGLRKQFR
                     CLQVGNLELFDQKNESVLTFTRKFGKEIALIAINFTQFPQETDCKELGSEIIWTKSFS
                     NKSADISQPKDGILRLVGEQAVLLIGK"
     gene            27940..29397
                     /locus_tag="PK898_03860"
     CDS             27940..29397
                     /locus_tag="PK898_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19199.1"
                     /translation="MMKKTKSWGVLLFLTFLFIAGRLDAYGILRDSVAANDTSSYFDC
                     AKADFTKIEAYECHRSATLPFIIQKLNPDLKYELTDVSEPFFGSEAELQVQPGTEKLV
                     IFQTLLSIFSWCFFTLTVVGLFRHELSKIFAAIILFGFAFVPQIADWDSILLSESMSF
                     SFFILLCAFLIRFLQNCYGPGSLMRRFLSGFFLLISAIFWLFTRDTNAYFIAFLSVIF
                     FILFWVTVFSHRKFQGLMLVLSLCLAACFLFQQQTFRTSKRWLLPLLNNLTTNVFPYP
                     ERVAFFEEKGMPVNEAVLSQSGSAEYNDLYNQKEFIQWAETNGLSAYTAFLMNMPLWS
                     VQQIFLNLDGFFAENLQPFFYGKPDEKPHWADKVGNVLHPLSSAVLWIDLFLLLILFI
                     YTIRKRDADSLVWFLFSFLLTIGSIMMISVAYLGEVRSVWRHVLCGVMPLRLILWIQI
                     LEILDQSLDSRPSPPIINRLGGQSNRSETDKILDR"
     gene            29434..30192
                     /gene="tpiA"
                     /locus_tag="PK898_03865"
     CDS             29434..30192
                     /gene="tpiA"
                     /locus_tag="PK898_03865"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU19200.1"
                     /translation="MRRPMVAGNWKMNKTAAEAIILVQELLPELQKQTAIDRVICPPF
                     TALYPVSGLLKGSGIGCGAQDVFWEEKGAYTGEIAPNMVAELGEYVIIGHSERRTYFY
                     ETDETVNKKTLAALAAGLTPIVCVGETLDENQSGRTKEVVSRQVREGLKGIPEVYVEK
                     IVIAYEPVWAIGTGLAATGKDANRVIAEDIRKPYADLYGEKAAQSIRVLYGGSVKGNN
                     AAEFFGQPDIDGALVGGASLKAPDFMEIVNAAAK"
     gene            30667..>31622
                     /locus_tag="PK898_03870"
     CDS             30667..>31622
                     /locus_tag="PK898_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOU19201.1"
                     /translation="MNILVVVGGGALILFLAYLTYGKFISTKVFELDNSRVTPAVEME
                     DGVDYVPTDAKYLAGQHFSAIAAAGPVTGPIIAGIAYGWMPTMLWILLGSIFIGGVHD
                     MGALVASIRHKAAGIADTMRQYVSKRVWILFNLFIFFTLVMIIVAFTDITTSSFINTV
                     TLDDGSVVGGGATATSSILYLILPVIMGFLLRYTKLSLLWATIIFLPLVALSIWVGQY
                     IPLNYGNKNVWNLIVILYCIIAGIVPVWALLQPRGHLGGYFLYATLVFGFIGIIFGGF
                     DTQFPAWTKTYGSSDFWTPMFPMLFITVACGACSGFHSLVGSG"
     assembly_gap    31623..31722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31723..32546
                     /locus_tag="PK898_03875"
     CDS             <31723..32546
                     /locus_tag="PK898_03875"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HOU19202.1"
                     /translation="ITVACGACSGFHSLVGSGTTSKQLAKESDAKPIGYGMMLAESVV
                     ALMALSTVMILTKDDPILTKSPNFIYANGLGSFMQLLGINKAFGISFGLMAFTTFVYD
                     TLDVCTRLGRFIYQELSGMKGLMGRIIGTVLIGGIPLILMSINLTDPDGNKVAIWSLF
                     WKTFGSSNQLLAALALVGITVWLQRTAKHRKAWIATVIPAAFMFVMSTWALVNTFLSY
                     TYVKGAWVMPVGPNKVVPGLSLIYIILAVWVVIECVPAIVKNAHYQDRQPVTQEA"
     gene            32737..34014
                     /locus_tag="PK898_03880"
     CDS             32737..34014
                     /locus_tag="PK898_03880"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HOU19203.1"
                     /translation="MVKKFYQLTATERIKELIDLKVITEEDAALLLKRGGLSSEVADH
                     MIENGIGIFDLPLGVAQNFMVNGRSVWVPMVIEESSVIAAASNGAKIVLQNGGFTAKA
                     DPPRMIGQIQVINVPDPVPAKRKVLRHKQALIAKLNENRPSLVNRGGGVLDLSGKIFR
                     KSAVGPMLVFYLTIDVRDAMGANLINSALEEIAPDIAKLTAGDIRMCILSNYADKRLV
                     HASCTIRPETLAKEGQAGEEVVERILAANALAEVDPYRAVTQNKGIMNGVDAVVIATG
                     NDWRAVEAGAHSWACKKGKIRPLSSWTKDSDGSLHGELEMPLAVGIVGGATRVHPLAQ
                     FSIRMMKIRTSCELAEIIACVGLAQNLAALRALSTEGIQKGHMALHARQIAITAGAQG
                     ADIDRVAETMCKEGNIAVDHAKEILKRNEITNS"
     gene            33995..35050
                     /locus_tag="PK898_03885"
     CDS             33995..35050
                     /locus_tag="PK898_03885"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOU19204.1"
                     /translation="MKLLIPENPVGIIGYGAYIPRYRIRASEISRVWHGDSEGTPIKE
                     KSVPGMDEDTITMSIEAARNALARAGIPATELRAVWIGSESHPYAVKPSGTVVAEAIG
                     ADVSVLAADFEFACKAGTEAMIAGMGLVGSGMGKYALAIGMDTAQGKPGDVLEYTAAA
                     GGAAVILGPADEALATIDAAYSFVTDTPDFWRRQSEKYPEHTQRFTGEPAYFNHLEHS
                     IQTFFDETHTKASDYRYAVFHQPNSKFPVKLAKQFGFKKEQYETGLLSPMIGNTYSGA
                     VLLGLSTIFDIAQPGDRILAASFGSGAGSDTFSLVVTDAILSRRNKAPSTQKYIDRRT
                     EIDYAMYARYRNQYLMK"
     gene            35065..36201
                     /locus_tag="PK898_03890"
     CDS             35065..36201
                     /locus_tag="PK898_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOU19205.1"
                     /translation="MNRIDHRQRPVILGTGILPIGQHWEISLRQLGGRAVRMALQDAG
                     NPKPDAIYIGNGLGTILSHQANLGALIPEFAGYPGIESCTFEAAGASGAAAFQAACFA
                     VESGYVDVAVALGVEKITECEDRTAEAAMNLALNYEFESSQGLTVKTEAALIAQRYLY
                     EWHLKREIFQNVALNSYQNARGNLNAFARNIDEKAYQKQPMISGPLGLYDIAAQNDGA
                     AAVVLTREELVPAEEQDRLIIVSGSGNAIASLALHDRHDFLHWSAAGQSAATALRQAD
                     LYLSDIDIFEIWDGTVMDVILSLESIGICQEGKGWQIDSLPLNVTGGCLGRGNPLGAS
                     GMYQIAEVARFLQEQKLENGIHRNALIQAIGGYGATAVTHILRS"
     gene            36358..36759
                     /locus_tag="PK898_03895"
     CDS             36358..36759
                     /locus_tag="PK898_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOU19206.1"
                     /translation="MESPRHWRLKKQRYALIGEECPHCKAKIFPPRDVCPECGLEAKT
                     AYQFSGKGKVYSYTTINEAPSGFQDMSPYAIALVKLEEGPIVTAQLTDLGDESVWVGM
                     PVEMVTRKLRNDGDERGVILYGYKFRPAFQA"
     gene            37011..37820
                     /locus_tag="PK898_03900"
     CDS             37011..37820
                     /locus_tag="PK898_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOU19207.1"
                     /translation="MNVSKRLDEARKAYDEKNPEASKKIHDEKVIEMAVEQHGSESSQ
                     FIGNMVYGGLDGIISVFAVVSGVAGASLTSGVLLILGAANLLGDGISMGIGAYLSMKS
                     EKEYYRKELERESWEAEHYPEGEKAELLAIYKNKGFPKEDAEKLVEIQSRDHDRWVDA
                     MMINELGMIKDERKPTSAALMTFGSFVLFGLLPLLVYLVDTIFHTNLSMSTMFLISII
                     LSGASLFGLGAAKYFITRRNPLVSGLEMLIVGGLAASVAYFIGVLLKPLGG"
     gene            complement(37995..38441)
                     /locus_tag="PK898_03905"
     CDS             complement(37995..38441)
                     /locus_tag="PK898_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19208.1"
                     /translation="MNQEIPGNIALGLNLGGLGGALFFLANIYTILHLIQRIFAPKAE
                     WKWLNNLRNKWHYVHYFGNIAAFVVIVIHAITLWQYATVFNWILIIVMAWMVFAGFTM
                     RFTKAAPQFKKTIRKYHAMWYMLALVLVLIITAHVVSLSSFPYPVG"
     gene            complement(38713..38874)
                     /locus_tag="PK898_03910"
     CDS             complement(38713..38874)
                     /locus_tag="PK898_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOU19209.1"
                     /translation="MKKYKCSVCGYIYDPAKGDPESGIKPGTAFEDLPDDWVCPVCGS
                     SKDKFRPID"
BASE COUNT        10560 a         8313 c         8979 g        11384 t
ORIGIN      
        1 ccggcacagc gaggtttgta ttttgtatgg aatgatccgg cagtctcggt ggttttgagc
       61 ggaatgggag cgttggagca ggtcaaagaa aacatcgccg ccgcggaaaa cgccgcaccg
      121 ggcattttgt ccgaaaaaga gctggctctt tattctcgag ttcgggacat gtattggaaa
      181 cggattatcg caccttgtac gggctgcgaa tattgtatgc cctgcccgca cggagtcaat
      241 atcccggaaa atctcaatgt tcttaatgaa ctttacatgt ttgataactt tgcggaaagc
      301 aagcgtgctt atgaattttt acccaaagaa gccagagccg aacagtgcat taattgcgga
      361 acatgtttgc gaaaatgtcc gcaaaagatt caaattccgg ttcatatgcg tgccttggcc
      421 gatcgcatgg cttgaaatcg acaaaaaagt ccctttcaaa tgataaaaag ggactttttg
      481 tttccgaaag agaagattat ttagcgcaaa ccgctcagtt gcttattaag attaatagct
      541 gtctcccggt attttgcaag cttttcacgc tctttttcaa cgacggcagc cggagctttc
      601 atggaaaacg gagaatttaa aagtttctca agccgatcga tctgttcatc ggtctctgct
      661 aattcttttt gcagacgatc ccgttcggca gccaaatcga tttcttcttc gatttccaaa
      721 aagataccta taccggaagc cgcagttgag atccgatccg cgggcggtgt atccaaatgc
      781 ggaagaattt ccagttgatc gggatcgaga tgtgccaatg ttgcaattgc atcccgttgc
      841 gattcaaaaa cagctgtttt ttcgcccgtg acgatcgtcg ccggaatcaa tgttcccggt
      901 ttcacatttt tttcggtacg tatattgcga acggcccgga ttacatcttg aatcatcagc
      961 atatctttga cggcaagttg atccgattca ccggttgatt cagcttcagg ccatttggcg
     1021 ataatcaagg cttcttccca accgtgcgcc ggcgcataag cttccgattg atcgatggcc
     1081 gctgttttta aatgtcccca taatgcctcg gttacatacg gggtaaaggg atgtaacaat
     1141 ctgaggcagg tatccaaaat ccgcaccaat aaacgagcgg tgttaaatgc cgtatttcct
     1201 ccccggctta attggatttt ggcgatttcc aaataccaat ccgcatattc gctccagaag
     1261 aaatcataaa tctgccgacc ggcttctccg tattgatggc tgtcaaacaa gcgttcaacg
     1321 tcagtaatca gattttgtaa acgcgcccaa atccaacgat ctgccaaagt cggctggatc
     1381 gggctgccgt cgtccgctcg actcggcgct ttatcgagcg ctccgataac aaatctgccg
     1441 gcgttccaga gcttgttggc aaaatttcga ttcgcttcca ccttcttgac gctcaggttt
     1501 gtatcattcc cggcggtcga tcccaccagt aaggtgaaac gtaacgcatc cgtcccgaat
     1561 tgatccataa cggtcaaagg atcaaccacg tttcccttgg ttttgctcat tttcatccca
     1621 tgttcatccc gaaccaatcc gtgcagataa acttggctaa aggggatctt gccggtgaaa
     1681 tagagaccgc tcataatcat tcgggcgacc cagaaaaaca gaatatcata tccggtctcc
     1741 atcatagtgg tcggataaaa atatttaaaa tcaggcgtct cttccggcca gccgagagtt
     1801 gaaaaaggcc acaaccacga agaaaaccag gtatccaata catccggatc ctgttcgata
     1861 ttcttactgc cgcatttcgg acaaacttcc aaatccgtcc gcgaagcgga aacttctccg
     1921 cagtcctgac aataccaaac cgggatacga tgcccccacc atagttggcg gctgacgcac
     1981 caatcctgaa tattttccaa ccagttgtaa taaactttgg taaaccggtc cggaatgatg
     2041 gtgatactgc cgttgcgaac ggcttcaagc gccggttctg caagcggttt catcttgaga
     2101 aaccattgag tcgaaatcat cggttcaatg atttcgccgg atcgttgaga acgcggaata
     2161 ttcatcatat acggttcttc tctgatgacc aacccggctt ctttcatatc cgcccaaatc
     2221 ttttttcggc attcaaagcg gtccattccg gtgtaagggg cgcccgcttc gttaatcttg
     2281 gcatccttgg tcaaaatatt aatcatttcc aggtgatgag tttcaccgat tttataatcg
     2341 ttgggatcat gtgcgggagt gattttcaca acgcccgtac cgaattcacg atcgacatat
     2401 tcatccgcaa taatcgggat ttctcgcccg actatgggga caagcgctgt cttgccgata
     2461 tatttttgat accgttcatc ttccgggtga acggcgacgg cggtatcgcc aaggattgtt
     2521 tccggacggg tcgttgccac cggcaaataa tctccgcttc cgtccgccaa catgtatttg
     2581 aaataataaa gtgttccggg ttcttcggaa tattcgactt ccagatcgga gacggcagtc
     2641 tgaagcttgg gagaccagtt tacgattctc ggtccgcgat acatcaatcc gtcttcgtaa
     2701 aatcgaacaa agacttcgcg tacagccttc gaaaggcctt catccaaggt aaatcgttca
     2761 cggtcccaat cacaggaggc accgagacga cggatctggg ctacgattcg atcatgatat
     2821 ttctctttcc actcccatac tttttcgatg aatttttctc ttcccagatc ttcccgttta
     2881 attccctgcg catttaaagc ccgttccaca accatttgtg ttgcgatacc ggcatgatcg
     2941 gttcccggga cccaaagtgt gggcattcct ttcattcgat gatagcgaat cattaaatct
     3001 tccatagaaa caaacatggc atgcccgagg tgcaattcac ccgtcacatt cggaggaggg
     3061 atggatatga cataaggagt atggtcggga ttaaaatccg gtttattggg atcgttccac
     3121 ggcttaaaat atccttgttc ttcccaccat cgatataact tttcttcatt cgctgcaaaa
     3181 tcatatgttt tcggcagttc atgttggttt atatttgttc catccatttt tcctcctatt
     3241 cagaaaaaac actttcgtcc gaaagaggac gaaagtgtta aatttccgcg gtaccacctc
     3301 aattcacacc ggttccaaaa aacgggtgtg ctctcttgtc tgagattgat aacggttctc
     3361 cgccgtgctg ttctactggc tataaatcgg atagcgttct tcagcaaatc ttatcggatg
     3421 acagttgtcg tatatttccc tcgggattct cagctgcagc ccttctctgt cagggtattt
     3481 cgacaattta atccgacatc tcttacgata tattgaggca aattatacag aaagtacggc
     3541 ttaccgtcaa ccgaagattg atgaaaggtg atacttattt ggtcgacgtt ttcttcagtg
     3601 attgaatacc ggcggataag ggataaaagc gaaaacaaag gaatggatat cctcaatcag
     3661 gaatctggat gggatgaatt atctttgttt tgatcttccg atttatcttc atggctaaca
     3721 ggttcttccg aatccgaaga agagggtaat ttctcgattc cggatgaatg atcggttgat
     3781 ccggattggg cagagtcacc gggagtctct atttctttcg gcgtttcatt tgtttgtgcc
     3841 ggaggttgtc cattcggatg gccggcattt atcgggtaac caaactgagc ggtgggtcgt
     3901 gagaaaaggg aatttcgctt gaaaacggaa ttcgaaagaa ttcgttgagc ttctttgaaa
     3961 aggtcaacgg catcggtatc cggaattgct ttattttcgt tgctgttcca ccaaaagata
     4021 taccccgcaa cgaggacaaa aataagcgga acaatccaaa tcatgcgaac tccttttgtt
     4081 cttctaattt attcaacagc tgaataattt cggttgaaaa ttgtctgtag cgccatggaa
     4141 gatcaaccat aatcgattga agttgtgctt cgttttgcgg atttttacaa ataatttcct
     4201 ccatgatatc acgaggaaga attacatcgg acggaacatc atatttgatc ccggtttctt
     4261 ttcgccagtt tctcaggtgc tcacgccttt gtaatgttga atcgcacggg atttttgtct
     4321 ttggcgggcg gggaatacaa cgaatgtcac gatcggtggt tttgaagatt ttcattaaat
     4381 ttttcccttc tttttcgatc agggatttcc gcattccttt gatatttgcc aattcttcct
     4441 ccgattgcgg aggattcaag caaagctcca gcataacatg attttgcatt actttgaaaa
     4501 cggggatatt catgtttttg gcgatccgtt cgcgccactc gcagacagcc tgcaacatgc
     4561 cgatttgctg cacggtcagt tgatgattgt ttccggcgat attccaccaa tttgtcgtct
     4621 ccggggcata ttgtccggac ggagttttac aaacccgatc aaaatcttcc tgcgccagtt
     4681 ccgtcagatt ccgttcagat agttcttgct ccaatttgtc tttcagttca attaaataat
     4741 ccgtatcatg aattgcatat ttcagcatat cttcgggaat gggacgaatt ccccaatttg
     4801 ctttttggta ctttttttcc aaacgaattc cgagttcgga ttccagcatc gcggcaagac
     4861 cgacttcttt tcgccccaga atcctcgctg cgatcattgt gtcaaagata tgattgaatt
     4921 cgaagccgaa gtcacgtttc aaacacatga tgtcgtattc ggatgcatga aaaactttta
     4981 agatcgactt cgaagaaaaa atcggagcga tcgaacttaa gtcttttaat gccaaagtat
     5041 ctaaaatcgc atgattttct tcactggaag cctgtaataa gcaaacttgc tcataatata
     5101 cataaaggct gtttgattcg gtgtcgactg ccacttcgga acatttttcc aaccatgcag
     5161 cgacctttgc caaagttttc ggtgtatccg caagcgtgac attcattgtg tctatggtta
     5221 tattcatgcc caaattataa tgcagtaccg gccatgtcga actttgtccg gatcagcgag
     5281 tgaacttgcc tcggattttc tgccgaagga aagaaatttt aattcggaaa aatccgcggg
     5341 acaaatgcat tgtttttcca aatcatgccg tataatttaa tcagagctta aaattggaaa
     5401 aaactcggac caaaaaactt tttattttct tcaatcccgc agccggccat gaaaaaaagg
     5461 tcgatctgtt tgaaaacaga attcgagaat ggggcaagaa taacgggtgg cggattgata
     5521 tccatcgaac tcataaaaac gaagatctga aaaaagcatt gcgccaacgc caaaaagacg
     5581 gaatcgatgt gatcggtgtc gccggaggcg acgggacggt ttccgaagtg atttcagcga
     5641 tggcggggat cgaccttcct ttactgatta taccggccgg aacgggaaac atttttgccc
     5701 gaaattttta catccctttt aatttggaaa aatgcctcag tttactgaat gaaggcagcg
     5761 aagtcggttt tgtcgatctg atgcttgtca acggctccta ccgaatgctt aatgccggtg
     5821 cgggtttcaa cgcccgatta atgatttcga cgacccgtga cgaaaaacaa aaactgggat
     5881 tttgggcata tttaaaaaat atcggaaagt cactgaaacg tactcatcag gttgaatttg
     5941 tactgactat tgacggcaaa gaagagagaa ttgtgggagc ggatgtcttt attccgaatg
     6001 caggttttgg catgcactct tggattgaag aaaatctatt aaaccccaac gatggggtaa
     6061 tttcggcttt tgtcacccgg ccgtctgatg tgcagagttt tttccgaatt ttatgggatg
     6121 cgatcaaggg aaaaagccgg ggggttgagg ttatcagtcg attcgagttt aaagatgagc
     6181 ttttggtcga aagtgatcgg ccgttaccgg cacaatccga cggagaaccg atcggaacga
     6241 ctccgttgag aataaaagtc gtgccctcct cattaaaatt gattattccg gttcgggaaa
     6301 gcccgttggc agggtttgaa tccatcaata aatatttttc tcggatattg aatttaccaa
     6361 ttttagaaga acagacatct ttaaagattc ccaaaaggat attttcggat caaaatcgga
     6421 agtgaatttt tagcataaaa cttgcactta attagacagg atttttcgaa aatttgaagg
     6481 gatctgatga gggtggatgg cagggaaagt tttacgatct aaaaaaggaa ttcttctttt
     6541 attactgctc tctgtcttta tcggatcttg taatacagca aagtccgatg atgcattgga
     6601 acaggaagtg gcttcttttg tgaagaccct gcaaatgcct accggagatc cttggcttgc
     6661 agccattcag ccgacacttt taccgtccga tacagcttct ccctcaccca tcctgttgtt
     6721 aaataatcaa acgaactcgg ctccgaccga aacggtcttt attccgaccg atgaacccac
     6781 cgctatcccg gtaccgccga cggaagtgct gtctgccccg atcgatacat ccaattcccc
     6841 gactgaaact ccgcttcctt tttatcggcc ggtgatcgtc gatccgaacc catcgggagc
     6901 ggattggaag agcctgccta tcattccgga atcgataacg gagaccgcga aagatattta
     6961 tcggtacgga gtgtcgaaga tgggaaggcg cccgaattat ttttccaaaa tcggggattg
     7021 ccagtcatac cccaatgtat tcatgggcga gtacgattat gtttccggct cttatgaatt
     7081 aagtgatgat gacctttatt tggaagatgc catcaattat tttcaagaat cgttcggcgc
     7141 tttgagtttc gcggtcgcga acggtctgag cgccgcttct ttgctgacaa ccatctggtc
     7201 tgacccctct tactgtgaga gtggtgagtc cgcattgctt tgcgagctgc gaatcaataa
     7261 accttcgatt gtttttgtta atttaggcac gaactgggta tcgaccgcag atgtatcgtt
     7321 gtatgaagat tatttggatg aaattgttta tacgattatt caatatggaa cgttgccgat
     7381 tctttcttcc aaagcggata atgtggaagg gaataatcag atcaatgaga tcacagccgc
     7441 tatcgccaaa aaatacggtg taccattttt caatttttgg gcggcagccc aaagtttgga
     7501 aaatcacggc ttggatccgg atcgttccga catttatctt tcagtcgatg catggaaagt
     7561 ccgcagctat tggggattga aaatgttata tacggtcggg cattctttgg aacttttttg
     7621 atctggggaa gcgatcgaac gatttataat agcaggggga gaaagttatg aaaaaaataa
     7681 tttattcgct cgcaatgatt attctgtgtg ttcagctttc cggatgtggc ggaaataaaa
     7741 aagtttccga agaatccaag gtagtggaat ctttttataa tgcgatcgtg aatcaagaac
     7801 gggacaagat cggatcgatc gtttgttccg actgggaaaa agacggttta cgggaagtcg
     7861 atgctttcat gggagtgaaa tcggagttaa aagatttcag ctgctccgtt caaaaggaag
     7921 aggctgacaa tgccgaagtg acatgcacgg gatcgatcgc cgcttcttat gggaatgaaa
     7981 taacggaatt tccgctgaaa gacagaatcc ataaagtggt taaagaaaac ggagaatggc
     8041 ggctttgcgg gttcgaatag gatttttcag tgagactgtt caatcgtgaa acattaatcg
     8101 ctttcctttt tgctcttctc attatcggct taatcatttt tacagcggat gaagccccac
     8161 aatgggttta tcagggattt taaatgcttt tgtcaaatat tctgttattg gcggggttat
     8221 ttttattaat ccgcggcatc cctcagcaaa gaattcgaaa gtattttctg ctggcgttat
     8281 cgatcatttt tgtattcagc cttcaaccta ccctttccat ccgtaatttt gatttctggt
     8341 tacctctcac gaccaccggc ctaacccttg tatgttgggc attttgttgc agaaatgata
     8401 ccagccgact ttccgataat cgatcggaac ttttgatcat tttcggaacg gtattgttta
     8461 tcgctttatc gcgtctattc agttttgagg gaatttttac cgcatcccgt cccccgatcc
     8521 tttcggtttc gcttccatgg atcgcgggct ggtttatcct gttttttatt ttttattatt
     8581 tcggcggttc aaaaccgaaa aaactttttt cattattcat cggattcatt ctgctgattc
     8641 tgattttgtt aaaaacaccc cggttagctt tacaagtcag cagatttttc agaaatttag
     8701 cgggtcaatc tgctgagctg gcgacggcaa acgatttacg ttggtttgga ttttcttaca
     8761 ttgcctttcg gcttttatcc gtgttaattg atgcccggaa gggtcgaaaa tgggttgcga
     8821 cacccggtga tttcctcatt tatgtctgtt tccctcctgc gttggcggcc ggacccattg
     8881 accgattgga tcattttgat aaagaactca actcagttcc tcatcctttt ccggaagatt
     8941 tcagggaggg aataccgcgt attgcgaccg gcttatttaa aaagtttatt cttgccgatt
     9001 cattggcact ggtcgcttta tcttctcaaa atgccgtgtt gataaagcat accggatggg
     9061 cttgggcttc tctgtttgca tacagtcttc aactttattt tgatttttcg ggttattccg
     9121 atgtcgccat cggcctcgct catctgatcg gaatcaaatt accggagaat tttaatcatc
     9181 cttatctgaa gccggatatc gcaaaatttt ggaacagttg gcatattacc ctgactcaat
     9241 ggattcgttc ttatacattt aatccgctcg tgcgtaaatt acgaaagaaa aaagatccgg
     9301 cttttccgca atggtttatg attctgataa cgcaatcggt aacgatggga ttgatcggac
     9361 tttggcatgg agtcactctt aattttttca tatggggcat ctggaatgct ttgggattgt
     9421 ttttccacca gctatatgcg gatcatacaa aaaaccaact gacggttatt tcggaagagc
     9481 actcgatcgc ttttcgaatc tatacgatcg gatcgacctt gttgacattc ctctttgttt
     9541 cattgggttg gatctggttt gtgttaccga atatcgattc ggcggctctt ttcttcgaaa
     9601 ggttattctg aggctttcat gaaaaagatt ttatctttta ctctaaaagt attctttttc
     9661 ctgatcatat tgcttgccct ctattatttc attcagcccg aaccgttttt gggaaaaatc
     9721 agtctatata atttcatatt tcccgggcgg gaacgccttc cgttcgggga aaacccgcgg
     9781 gtagcctata atttcagtct ttacaatttg aacgcgatgt tcgcatccca tgaaatttcc
     9841 ggtgtacaag aacaggataa ttctttccgc gtcatcacca tcggtgattc ttccgtttgg
     9901 ggaacactcc tgaagcccga ggaaacactt gcaggacaac tggatgggaa aaaaatcaag
     9961 attcacggtg ccgaaagaaa attggaagtt tataatctcg gttacccaac cctatctctg
    10021 gcaaaagacg tgttgatttt acagcgcggg atggcttaca aaccggatct tattttatgg
    10081 tttaccacta tggagtcatt tccgaatgaa aaacagatcg aatccccttt gctcgcaaag
    10141 aattcggagg aatttgctca actgttgaca tcttacggtg agattccgag ttggatgccg
    10201 aagtcttctc ccgaaagctt ttgggagaag actttgtttt cccagagacg ggcactgatg
    10261 gacctgatcc gtttacaact ctacggtttc atgtgggctg cgaccggaat cgatcaagat
    10321 tatccggcgg attaccctgt ggctcaagtg gattttgatc gggacaattc ttttcacgga
    10381 gtggacggaa tctatccaaa cgatcaaatg gcatgggaat tgttgaatgt cgggatgaga
    10441 atcgccggta atgttccggt gatcttaata aacgaaccga ttttaatcag tgatggtgaa
    10501 aattcggata tccgctataa tttttactat ccccgtcagg cttatgacgt ttggcggaaa
    10561 gatttggctg atcgatgtac cgaagaaaaa tggaagtgcc tggatctttg gaatttattg
    10621 tcccaatctg atttcacaaa cagcgcaatc cattataatt ctgaaggagg acagaaggta
    10681 gtcgatacga ttttggatgt cctccctgaa agtttgcaat aaaaaataaa aatgaaaaag
    10741 cctccctcgt gtagcaaaaa tctctttttt ctgattcttt cgctgccctt ctttttttcg
    10801 gttccatctt ttgcacaatc catcgaagat cgtcgtcctt ccgattttgg cagaacttgg
    10861 caagatttgc cgatcgtacc ggaattaaat gagaattcgc tgcgaatttt gaaagccgga
    10921 atcgctgcgg gtcataatcc gcatgctttt tcaaaggtcg gtgattgcga taccagtacg
    10981 gactggtatt tggcggattt tgataaagat tcccgatact ataatttggg cgaatataga
    11041 gatcgatttg aacctgtttt atcttattac aagggatcat tttctcatcg aagtttagcc
    11101 gcaaaacccg gtttttcggc tgcttcggta ttgtccaatt attggctgga ttttaattct
    11161 tgcgagcccg atgagcttcc gctcacctgc gaatatcgca ttcagaatcc tctgattgta
    11221 ttgatctcgt tgggtacaaa tgacggatat aatccgccgg cattcaaaga gaatttaagg
    11281 acaatcattg aaatcacttt atcggaaaat cggttgccga ttctgatgac caaagccgat
    11341 aatgtcgagg gagatttttc catcaatcaa gatatcgctg atttggcaca ggaatatgat
    11401 ttgccgctct ggaatttttg ggctgccatt caagatttac cgaatcacgg acttgtagag
    11461 gatggtattc atttaacttt ttataagaat gattttgccg atccgaatgc atttaattat
    11521 gcatggacct atcgtaattt gacagcattg caactgcttg aaaagctgat gcaggcgaca
    11581 gagaagttgc aaaataaata agaggaataa atgattgaga tagtagatat acttattgac
    11641 aagatcaaaa atgagattct gaatcaaccc aatcgaaaat taaaatcgga tacaccatta
    11701 atcagcagcg gtctgattga ttcatttcat ttagtcgatt tggcgttgat gatagaagat
    11761 acattcggtg ttcatatcga ggattacgaa ctgcgatccg atatatttga caccgtggat
    11821 gatcttgcgt tgctgattga ttcccgcatg tcttaatcca ttaattttct gtttcaggcg
    11881 aatcatccgt aacccgcaaa ttggtccaga tcaagcggtt gaagcgggat tccttccgga
    11941 aaatgttgcc ggtaatattg aatcgctccg agcatcggcg ggaagatttt atcggtggga
    12001 atattccaac tggcaaagaa acgaaattca acaggtttct cacgagaaat gcggagcgaa
    12061 ccgtgcacac tggtcggaat aaagaccgca taaacgggtg taatatttgc ggaatcttca
    12121 tcaatataat ttaattcacc gtctgaaaaa agttgaatca acattccttt ttcgataaat
    12181 acgttcgtaa cggaaaggac ggttcgttga agacattcga aaacactttc atccggctgc
    12241 atcgccccac ccggtaattc ccaacagctg ttttcgtttt ttcgaattaa taacaaattc
    12301 ccttgcggat cggtaatata agtcaacgca aaaaattcca ccaaaggaaa gctgcctgct
    12361 aattgatgga ggcgagaatg atagtcaccc atattatgag aaatattccc ttaaaactcg
    12421 gctccggacc ggatgccgta atttccttag cgccttggcc tcaatttggc gaacgcgttc
    12481 gcgggttata ttaaatttct tgctcacttc ttccagagtc tgctcttctc cgtcgatcaa
    12541 accgaatcgg tactccaaga cttgacgctc tcgttccgtc aaaatatcca gcgttttcat
    12601 cacctgatct tttaaaattt ccttggcagc cgcatccatc ggctccaacg catcttcatc
    12661 ttcaagaaaa tctccgaatt gaccgctttc ttcatctccg atcgggtgtt ccagcgatat
    12721 gggctcttca gcggattgca gcagattctg aactttgttt atcgctatcg ataatttttt
    12781 ctgaagatcc gggctcagcg gtttgttttg ctctcgacag gtccttattc tttcgctgtc
    12841 ctccggatcc aaaaaatgag cttcgatggc aagttcttcc atactcggat ttcggccgag
    12901 ctcttgaacc aactttcgct ggatcttcat gaccttgttt acggcatcaa ccatatgagc
    12961 ggggatacga ataatgcgag cctgttctgc gatgtaacgg ctgattgctt gccgaatcca
    13021 ccaagtggaa taggtgctga atttgaaccc tctgcgcgga tcaaatttat ggacggcgcg
    13081 caacaatcct atgtttcctt cttgaattaa atccaaaaac gaaattcctc ttccaagata
    13141 tcgttttgca atactgacaa ccagacgtaa atttgcgcgg atcaaaatct gatttgccac
    13201 ttcggattgg gtctgaacct tgttaatatg ggtaatcata tcaaaggaaa ccggcaacat
    13261 ttcgattaga gttttctgat cgggaagtac attgccgttt gccttcaagt attcaagaag
    13321 tttatcctgc atgctttcag gcagcagata aagacccaga aaaatatcat aaaccggatc
    13381 gacgacctta taccaatccg gatcggaacc ccatcttccg ttatccagat aagagcgaat
    13441 ataagaaggg ctttcacttt tccaagctcg cagtaattgc tgcgcttccg ttgtcatttg
    13501 aactaaatcc ggcagcggat actcttcttt ataagcctta atggcttccg ttgatttctc
    13561 ccatgcttta ctgagttgga cacaaatctc ccgataaaga ctttcgatga accggtcttc
    13621 ggaaacatcg ccgattgccg cttcgcaacg gtccataaaa tcgatcgcgg cgatacaggt
    13681 cgcgagtcgg aattcgtcgt cggtacttaa taaatcaacc atcccgattt ctttaagata
    13741 cagacgaaca ggatcctcat aattggcgct gtctaaaagt aaatcttcgt ccgaccaatc
    13801 aagattcaga tccaagtccc ggtcccaatt cgccatatcc atatcctctg tgatatcgat
    13861 atcttctgcg atattttccc aatcatcctg cggctcattt tcagcctttt tcttttcgtt
    13921 ttttttcggt tcatttgcca ttgggttcct cggttaagcg ttgatcaaga cttttcagca
    13981 gtttttccag atttttcctc tcatggatca gttgatcgat taaacggtcg tactcttctg
    14041 gatttgatcc cgattcaagc tgctccttat acagagtctg gagttccaac tgattaaata
    14101 tatttttctc gtttcgcaga taggcgacgg aacgggtcaa atcaatgtcc aaatcgaccg
    14161 gattgatgct ccccttgaaa ttcggtttgc ggattttttc aaatgtcggc ctgatcgagt
    14221 ccgggatatt ctcgataata tatttttgtg tattgagctc atcatcctga ttaagcccgt
    14281 cgaaataaca gccggcgatc acacgcaaat ccgattcctc aaagtcttct ttgcgtaaag
    14341 ggccgagatt atattttctg aggttccgat cgatttttga aatagcgccg ggagtattat
    14401 agaaatgata taaaaattgc aggatggtca tttctttact gtaaattcct tctttcggat
    14461 caaaaacaag cgaaggcttc gattgaaaat cgaggcggtc cggaatttcc cattgagttg
    14521 atttctttga cttttgcgga gaaatataat tcagcagatt ttcatcgatc ttcagaaagc
    14581 gtgccaactg ttgtcgatag gtttcccgtt caatcggatc gacaacttcg cggattaatg
    14641 gcatgatttg cttggcaatt tcactttttg tctttgcatc ttccaaattt cgaccgtttg
    14701 ccaatgtttc catcataaaa atcacgacgg gcttggcact atccaaaatt tgttgccatt
    14761 cggacggatc ccgtaaaact acctcatccg ggtccattcc ttcggggatt cgagtaattc
    14821 ggatatcggc gttcagcttc cgttcttgac ggatcagcgt ttggctttcg accgtctctt
    14881 ctttatcgtt ttttacggcg gaacgaatca catcgattcc gcggatggtg gcgacatcac
    14941 cggctgcgtc ggcatccagt gccaagatca tgtttttgga ttggcgcgcc aataattttg
    15001 cctggtccgt acttaatgcg gttcccatcg gggatacggt attggtaaaa ccggcttgat
    15061 gcagaatgat tacatccaaa tacccttcaa cgatgaccgc ttggtttttc tcgcgaatgg
    15121 aagattttgc ccgatttaaa ccaaagagcg tctttccttt gtcaaaaagg acggtctgag
    15181 gagaatttaa aaatttgggt acatcgttcg gatcgagaat tctggctccg aatccgatcg
    15241 ggttaccgga tgaatcatgg atcggaatca tgatccggtt tcgaaagcgg tcaaaaattt
    15301 taccgtcttt aataatctcg cctttgtcgt ctcgttgctc actgacaagc ccgacgtcga
    15361 tcagatcctg ccggccggcg ttcatcgata aaagatgttg aagcaaagcg tcccaagaat
    15421 cgggggcata tcccatttcg aagttatgaa tggtctcatc cgtcagacct ctcttatgaa
    15481 gataatccaa tgccggtttc cctatgggat tatggatcaa attgttgtga taataatcaa
    15541 tggcattttg catcagctga tgcaaattat tgatttgttc tttttttgtt ttgtcttcct
    15601 gtttgaaagt ttccaacttg attccggccc gatttgccaa aacctgaagg gcttgctgga
    15661 aatcaagatt gtcgcgtttc atcaagaagc tgaaaatatc tccgccttcc gcacattggc
    15721 cgaagcagcg ccagctgccc gaatccggaa atacaacgaa agaaggcgta cgcgtattgg
    15781 gatgaaacgg acaaaagccg atataattct ttcccgttcg tcgtagtttt accgtttccg
    15841 agacaagatc aacgatatcg atctgatttt ttatttcatc aatttgtgac aatgaaatcc
    15901 ctctcgttca attttacaac aataatcgga cggcattgtt aagggacaaa gtaatcgatt
    15961 gggattattt gataaattta aaaaaagaac ttattgggcg gaggaagacc ggccggagat
    16021 ttttcaggaa cttataaaaa tttagcggtt cgatatcgga gcattttctc tattccgata
    16081 ggatgaagaa tttcatgatt cggtttgaag ggcatccgtc gattgatttt ccggtaagag
    16141 gaaattccga atctccgcaa catatcgctg cggaaagcgg ctgaataatt ctccgtgccc
    16201 caaattcccg aatattctga ttcgcatggc cggcaaatat tctttcattt tctttgctgt
    16261 ttttgcgggg tacggttctc gttggccgtg ccaaaaagtt acctgagctt tggtttgtcc
    16321 gacataaggc gagatttcgt attcaaaggc ggctttacag gcatttgtga ttgttttctc
    16381 ggacaaatta aaaaataaca tattataaag ggcttcaaca tctccgcttc cggtcacttt
    16441 tttcaaaaaa gaagagatat ttttaccgtc cgtaaattta gataacatag agatccacat
    16501 ggacgcatac ggtctgctga gacttcccgg tgtgcttgcg caagctccgt ccaagattac
    16561 ccgatccatt tggattttat tccgggtcaa taattcaatc gcaatcgttc ctcccaatga
    16621 aaatcccaag actccgctga gatggcgatc gaaatcatcg acaacctttt tttcaatttt
    16681 ttccgcttcc tcttggacgg tttgaaaatt ggatcggtta tccggattat gtccgtccag
    16741 caccggaatt accaaaaaat aatcatccat caataacgga actatttcgt cgaaagattt
    16801 ccaggtcatc gctaagccat gtagcagcag taaagaagga tttttctgat ttccgaaagt
    16861 tttaaaatcc atgaatattg tgcctttctt tcagagaatg aaacgaatat ttacccatta
    16921 taatcaagcc ggcggctaat caacctaaat gataattttg tttatcgatt gggaaagaac
    16981 cgataaaaac aattcgcaag cagaaacata taataaaaat tacgctgccg taaatcggat
    17041 aaggttatcc ggtgtatttc tccgatcgat gatcccgaaa atcaaaggat ccccgggctt
    17101 ccaattccaa accgtttcca aataaaaagg gacatcgggt accagctcta aatgtgccac
    17161 ttcgtccttg cgaaaccaac cgattgttcc ttcggaagag gaatgaagat gatcggctaa
    17221 ttgatcgacg gaccccttaa ataaatggat ttggattccc acttgctcgc cggtaatgtc
    17281 ggtcaaaatc ccgcacagtg tcagatttaa gagcgtcaat ccgcattctt ctttcaattc
    17341 acgacgtgca cattcaagta tcgtttcgcc tcgttccacg tgtccaccca atccgttata
    17401 aagatgcggg aaattctttt tatccggtgc tccttttaac agaagaattt tttcatcatg
    17461 aaatatgaag attaaagttc tgggaatgac ctgatatctt tccattttca gaaactgaaa
    17521 tccaagaaat ttgtcttggt aagaatggaa aagacgacga tgataaaaac aaccattaat
    17581 agacaaccgc agccgcagcc caagattccg ttcccgtaac cgaattttct ttgtgcccaa
    17641 tcataaatat ttttaactaa aaaaagttgc agcaaaccgc tcaaacaacc ctgtttttcg
    17701 tccttcattt atctgccttt ctaaaatgtg ttctgtgatt taaatcagaa agggcatcct
    17761 ttttctttac agggacaccc tttccggtgg aattgctttg tcccgttatt cgaggctgac
    17821 ggacagctga taattcgatg ccgctccaaa tgctaccaca tcgatcagat aatcctggtc
    17881 ccccggcaag gttccggacc aataggtttg aaaggcattg ttatccaaat atacaacacc
    17941 gtcacccaat cccataatac gtaaataggc ttgcggagtt gcaccgctgt ttaaattaac
    18001 ggtcattacc tgatctgcat gaccgaaaat ggtgtaactg acgacgctgt tgcttcgaag
    18061 tgttccggtt tttacggcat aactggtggg aatatttacc ggaatgacca gatttaatgt
    18121 gaagtcagcg ggttgctgcc cataattaca gacatccaaa aagtaagtgg cggtttgttt
    18181 caacggcatc atccaataat tcgatcgatt gaaactgttg agataaaccg ttccgtagat
    18241 gtccgaaaca ccgagactga ccgatccggt tgcggagttt aacgttgcga tcattttgta
    18301 gttattccct ccgaaaaagg aatagcggat gcaattgttg gctgaaacgt ttcctgaaat
    18361 ccatgcggaa gtttcacccg gggcaaattg aatcgctccg ccgttttggg atatcggata
    18421 tggcattacc ggaaccggtt gggttgtcgg aagaatcgga acggatggac cggtctgatt
    18481 gagaggcggg atagtaattt ggaagctgaa attggttccg ccgcctggat taaataaagt
    18541 cagataatag ggaccggtac tgtacagagg cgaaatataa tattgccatc ttttttcggg
    18601 gctcaggtag atatatcccg ttccgtcttg gataccggca aagacactcc cgttggtcga
    18661 agtgaccgca agggacagaa cttgattttt tgatgcataa aactgatagg tgatatcact
    18721 tcccgcatcc aaccacccgc tgatcgttgc ggaggattgg ccttcggcga attgaatcgt
    18781 gcctccgctc agggatttcg gttgggaata aaccggctga ccggattgaa ccggctgaac
    18841 ggggacttga ttcagcggcg gaatggtcag ctgcaaaacg aaatctgttc cggggccggt
    18901 atcttgcacg gtaagataat aagtaccggt ctgcgggagc ggcatcgtgt agtacatgta
    18961 tttacgatcc ggactcaaat aagagaaata gtctttggaa ttactgaacc cgatgatggc
    19021 actattgttt gcggatttaa ccgaaatcga aacgatctga ccggaaagcg cataaaaaga
    19081 ataccgaata ctttcattcg gtgccagact tcctgagatc caggctgaac tttctccggc
    19141 gccaaattga atgaccccgc cgtcggtctg tgccaaagtc gtaaaaatgc cgacaaacag
    19201 cgataaaagt atcgcaagag caaaggaacg gatcgttttc ttcatttaat cctcccgaaa
    19261 tattctgaat cggaatattt catctttcat ttattttact caatctacaa attttcaggc
    19321 tgaacgcttt caagcgtcag ttcaaaatca accgagacag aaaccgattt catttcaatc
    19381 aggaatttat gcccgtcttt tcccgattgc catgccagcg ctcggttgac attcaatgaa
    19441 ttttctttac cggaacttaa atcgtaaata tgaaaatcaa ccggctctcc gttcaagatt
    19501 ttcaaagaca gctttaaata ttttcccgcc ggaatctcta ttgtgaagcg gcgggtggat
    19561 gatgcgttga ttcgatccga taaatgaatg accgattgat tttttgaaaa agagagtctt
    19621 tcaggtaatg aaagctctaa gttgtagctc aaatcgtgat cgggagctgt gatttttagg
    19681 ttataccaac cgttttcatt caagttgaag aacatttcat ttgccgattg ctgcggttca
    19741 atgtttgttc cgatactgtt ttgcaaaatt aaatcgggtt ttgacagcga cgaagaagcc
    19801 aatccgattc gtaatacttg atctttgttt gcgataaacg tataagtttt ttcgctttcc
    19861 gattttattt ctcccgaaac aacgacggaa ttgtcaaact ccgaaaaatg aatcaattcg
    19921 aaattggaga tttgggtcgg cgtaacagtg ttcttcacac acgttccgtt attctcacag
    19981 acaatgatcg gagtgatgaa aatcaccgga atcggagttt cggtcggttt tacgaaaccc
    20041 ttcattgcgg tcggaatagc ggtttcggtt gccgcagcgg tcggtgaaag aaaaagcgct
    20101 attgtttttt ggagagtcgc ggatgcggtc cgggttgcgg tggatgtttc ggttggttcg
    20161 gtcacttttg ttgccgtcga aaaaagtttt gtcgctgttg ccggaatggg ggaagatgtc
    20221 ggaattgaag ttcgcgtcat ttccaaagtc ctcgtataag tcggtgaaag tgttacggac
    20281 ggaatagaaa gggtaatcag aaaagctttc gaatgatctc tttgcacttt gagtggagcg
    20341 gcggtagcta tcagtctgag ggggaccgct gtaggttgca aagtgacagt cagatcggga
    20401 atggcgatag gtgtcgaggt gaatgaatat tgcggagaag agtgattttc cgccgttttg
    20461 gtttgagtga ctgaaggaga cggctcaata cccggcaaga gagttgggtt ttcgggtgcg
    20521 gctttttccg gtgcggattg aaagtcagca gttacgaagg gcgtttgaat aatattctct
    20581 gtcggtttca ggataatttg tacggtattc gttccaatcg gaatttctgt ctgatcggtt
    20641 ttgcgattct tttccgagag aatcgccgct ttgggggtaa acaaactgca accggaaagc
    20701 tcaaacagga gcaaaaggca agaaaaacaa attatcccat tctttttcat aattatcctt
    20761 atgacgatat tcagacgctt aaagtttttg ttgatcgact tgctttattt ctgttaaagt
    20821 tgattcaatc tggtcccata tttcgggaat cttggtgata ttcattccgg ggctgaaggc
    20881 ataagcgaat ccgtcggaaa ggaccaagct gtctgtgttc caatctgcgg gcagcggaga
    20941 aggttgaatc gctgtccgcc attttaagga tgcataaatc tttgaatcgg ctttctcttt
    21001 aaccattttg gtgatttccg gggtatcctg caccggccag gcatttgcgg ccaaagccaa
    21061 ttcggtcaga ttttcgggtt tcattaacca cgaaaggaac agccaggatg caaattgttc
    21121 ttcggcggtt ccggtaaaaa taccggccga aacagccgtg gaaaagatta cgggagaatt
    21181 ttcgttgtcc gaattattga aagggtaagg gataagcgtc caatcatccg atcgattcga
    21241 atcagcgttt tcaaaagccc gtcgttgaaa aggaatatcg gcaaatgtgc cggaatagag
    21301 taaagcgttg tgattcgcga aataatcgaa cggttcggat aaacgacttt gccatacgca
    21361 accttgatca aacaattccc tcagccaagt gaccgtatcg gtaaaaagat cccggtcttt
    21421 ttcttcgggg aaattgctga aatcagcttt ctgaggggct gttgtcatca tccaactaat
    21481 aaccgcaccc ggcgagctcg tcaggatcca ccctcccgtt ccgtcattac cgggatccga
    21541 atcggaattg acggcagcca acgccgcaca gatttgttcg gtaaaatcat cacgatttgc
    21601 gggaggggtg gagaacccca gaatctgtcc gaaagtatcg ttataaaaaa ggaattcggg
    21661 taaatgccaa agcggaagtg cggtcaaatt accccgataa tattcttggc tcatcattga
    21721 atccataaca ggcggtattt cctgtgcttc gaacccgtag gtcggatgat ctatataagt
    21781 ttggagcatt accatcggga atttagcagc ctgccaactt ttaagccaag gagatccggc
    21841 aatcacgata ttgggaaatt catttgccgg gggaggcgtc attaaaacat cgttcagaat
    21901 cgagtcggaa agttcgttat ggagtgaaac cgtaaatccg tattcatctt cggaattgaa
    21961 gcgatcgacc agctcttcga gtttggattt tacctcgttt tgttgggcgt gccagactcg
    22021 gatggtcatt cccgcgacat cttccaatcc gagattaaca tgttgcggaa gcggtgtggg
    22081 agatgcagtc ggttgaacgg atggggtaaa aaacagtgtg ggaataattg aagcggtgct
    22141 ttgtgcgggt tcggcagaac tgccgagcgt tttggttttt tcggaagccg gaacggtctg
    22201 acaggcagga atcataaaaa acaagattaa gagaatgcct gcttgcaggc ccgctcggat
    22261 catctttttt cccgaatcgg gaggggaaaa gaatcggttc atcatctatt tataatatgg
    22321 tttttccgct ccgacacttg agaattcttc atgtcccggg aaaataaggg tttcatccgg
    22381 aagagttaga attttttcac gaatgctgtt catcagagca gccgggttcc cgccgagtaa
    22441 gtcggttctt ccgcaatcat gataaaaaat caaatcgccg gtgaacagcc aaccggcttc
    22501 tttttgataa aaacaacaat ggccgggagc atgtcccggt gtgtgcaaga cctgaaattc
    22561 atattttcct acggcaagtg tcatcccatc tcgaagatga atcgcaggct tcggacagta
    22621 tttttcttgc cccgcacttg aattgcttgc tattcctccg ttttcccgga taatttcgtc
    22681 atccggatgc atggcaatcg gaatttgggg ataacttttt acggcagatc gaattccggc
    22741 gatatgatca aaatgagcat gggtcagcca gattgcttta aaattccatt gctcccggtc
    22801 gatcttttcg agtacctgtt cgaaaaagaa tgacgggtca attgctatac attccttcga
    22861 ttttcggtcg gcgatcagat aggtattgtt caataacggt ccggattgaa acggtatcat
    22921 cgtcagatct tcatattgat aagaagggtt gttcataata ttcttaactc ccgcggaaaa
    22981 gatgtaattt gttcgcatcc ttcgtttgtt atgactaaat catcttcgat tctgacacca
    23041 aacttgccgg gaagatagat acccggctca tcggtaaaaa ccattccttc gcttaacggt
    23101 aaactgtttt cggcgtagat atacggatcc tcatgagctt ccattcccaa gccatgtccc
    23161 agccggtgcg taaagaaaga cccatacccc gctttttcta tttcatcacg ggcagcccga
    23221 tcaatcgctc cgcagggtat accgggacga gcggctcttt ttgcggcacg gttggcagcc
    23281 aagacagtat tgtaaacctc gtgttgaact tcgctgatat cgccgacggc aaaggttctg
    23341 gtaatgtccg aacaatagcc ttgataacgg gcgccccaat caaataagag aaaatcaccg
    23401 ctctccaaaa tccgatcact gaccgccgca tgcggatcgg cagcattggg tccgccggct
    23461 acgatgggag gaaacgggag cgtcgaatca cttcccgctc gcatcaactg cgcattcaac
    23521 tccgatgcga tttcgcgttc gctgatgccc ggtcgaacga ttttcaatgt cttctcaaga
    23581 gctgtttcgg cgataatcgc cgcttttttc atgaaagata tttcttcttc cgtcttttgt
    23641 aaccgaaatt ttttaaagag agactctccg gaaacaattt gacaatccgt aacggcattt
    23701 tccaaaagct tggtttcaaa gaagcgaaaa tgaagcgggt cgacggcaat tgtttttccg
    23761 gctagatcca ggtattcgcc cgcctgtcga aatgcgtttc cccactcgct cggattgtcc
    23821 gaaaaaggaa atgctctgaa attaaatccg atccggtcaa aagaattcat ttcgaatccg
    23881 gcggcaacta ttgcgggatc cttatcaacc gtcaccaata tcatggtagg tcgctccatc
    23941 agatgtttct ccgttccggt caaccagcgg aaatcggatc cgggaatcag agcgacggca
    24001 tcgaaccgat gctcgttcat aagatttttc aatttttcta aattcgtttt tgtcaaggtt
    24061 ttttccttta aataatattg atcaaaaata actattgaaa gatgctcact tttcagccgg
    24121 acaacgacac ccttctattt acagaactca ttttcgagcc atggcagggc ttttttggcc
    24181 ggagacggat cattcaagat accggcggcg attcgaaaat aacggcaggt ttcgaatttc
    24241 agatactcat ctgttttcag aatctcccca tagggcttgg catccgaata tctgccgagg
    24301 taagcatacg cctgtaccaa gggcagccat tccaacggat cttccccgtg agctccggct
    24361 gacagcgctt cgccggcgat ctccgcaact ttttcccaat cctcagattg ctgtgcgaga
    24421 gacatctttt catagaaata gcaccaattt ttatcgggtt ctttgccgaa gaattccgaa
    24481 gacggaacat gcggatccgc atcggtcaaa atcttatcta tttgagaata acttccgatt
    24541 tccgaaattc gtgaccattg cgccggcgta taaatcgggt tccttccgtc gataacttgg
    24601 aggcaggatt tcaccgtcgg tttggtcaat gccaaaattt gcgagtaatc tttgcgcaaa
    24661 tagatttctc ttacaattcg atcctctatc tttttcatat cggctttttt ggctgaatct
    24721 tgattcaaca gatccgaccc gatcacaaca tggtcctcat ccggatgata aatcaaatga
    24781 agcggaacaa aaagttcata atcctcttcg gcggaaaatc catccatact caacaggacc
    24841 aatgttcccg ggctgatatc gggcgcgcgc caagaaagct gccaccagaa gtcattcaca
    24901 acgtcggtct gtgcgatata gttttctttg ttggtaattt gggtcataca ggccgccaag
    24961 ataagaaccg cgaacagtgc ggtttttacc cattttcctt cgatggaagc gattaatccg
    25021 accaggaaaa taatcgcgga aaaggaagca tggtaggtaa aacgatccag actggagctg
    25081 aatgttatct cgcgatttgc gataatcatc ggggcgatag ccaaaacagt cgacaccaac
    25141 ccgatgacaa tccattgctg tcgccaacct gcttttttat tctttgatga agaagattcg
    25201 gcagagattt tctcttttct ttccggcatg agacggatga aaccgatgat gaaaattgct
    25261 gcggggagaa gtgaataggc aaatattttc caaaagcgtt tgacttccaa ttgactgagt
    25321 aagttataag ccggtatgat ccatgatcca aataacaatt tgaaagtatt ttttaagatc
    25381 tttgcgaccc agtccattcc gtaataacgg ggatgctcga agaagggtct taatgtatcc
    25441 tgaacgtcag cgccgctgcg agtcgcttca aaaaggaaac tgcgccaaag aaagaatatc
    25501 aaagctccga ccagccaggg aagatagttg agaattgtct ttcctacgat acggggaaat
    25561 cgatcatccg ccgaattata aaaataggcg ccgatcagca aaattcgtaa cgcttccaaa
    25621 ccgatataat attccatcag aaacatttcg aggattgcca gaatgatcga cagcaggact
    25681 gccggaattt tcagccgact tttcctcaat tccaatgaaa gaattgtcaa ataaacggat
    25741 agcattcctg ccagcatggc tgtttgatgc ggcaagtagg caattccgtc cacttgccgg
    25801 aggaagcccg gaaaaaccag aaacagagaa gctgcgacaa aagcgggaaa cttattcttt
    25861 ttccagatga gagtgagcgt taagtaaaag aagaaaccgc aaaaaaagcg acaggctaaa
    25921 ttaatcagca ggtaagggta tggattggga ccgaagagat cataagccgc gcttaaaaga
    25981 tacccgtcca ccggtcgatc gctctgataa tgccggatca acatttgtga cccttgcgta
    26041 taggcgttaa agacattgtt ccaatcatcc cgataatagc ccagtcgatt catcccgggc
    26101 agataaacca acaatgtaat cgcgaccagg atcaaacaga tgagtaaatt accatgtcgt
    26161 aaggatttgc ttttttcagc catgaatccc cctttgcttc tcttatagat gattttaata
    26221 tgattataaa acaaacggtt tgcgattttt gtccgagccc gaatgttata atttccaaag
    26281 agtcagaaaa aatcgattga aaaaaggatg aatgcgatga gttatatccc ggaaaaaaag
    26341 acggaatcac tgtggtggcg ggacggcgta atttatcaaa tttatccccg ttccttcatg
    26401 gacacaaacg gagacggaat cggtgattta aaagggatta tcggtaaatt ggattattta
    26461 tccgatttgg gaattgcggc aatctggctt tcaccggtga tgccttctcc cgatgcagat
    26521 tttggctatg atgtcgcgaa ttacaaagat atcgatccga aattcggaac gatggacgat
    26581 tataagaatt taattgccga agctcaccgg cgcgggatcc gaattattat ggatttggtt
    26641 ttgaaccata catctaccga acacccatgg tttcttgaat cgaaaaaatc gaaggacaat
    26701 ccttttcatg attattatct ctggcaggat catcccaata attggaaatc gacttttacc
    26761 caaagaagtg cctgggaata tgtcgaatcc tgcggtcaat attactatca tatgttcact
    26821 gttcaacaag cggatttgaa ttggcgcaat ccggaagttt actcggaaat gatgaatatt
    26881 ttccgttttt gggctgattt gggaacggac ggttttcggc tggatgtctt caacctttat
    26941 ttcaaagatg atcagtttcg aaacaatccc ccggcttttc attggattcc attcatgcgg
    27001 caaaaacata tttatgattg cgatcgcccc gaaatggatc gggcagttcg ggatattcgg
    27061 aaagttacaa acgcttacaa agacagttat gtcgtcggtg aaacgttttt gagttccggt
    27121 gaaaagatta agcggtattg cggtgaagac aaacttcacg gggcttttga tttcacattt
    27181 actcattgca aatgggatgc ttcggctttt cggaaaatca ttaaaaacgt cgaacaaaac
    27241 tcacaggaag ccggtttctg gccgacatat gtcctgaaca atcatgatgt ggaacgatcg
    27301 gcttccagat tcggcttggg agaatatgac gaacggctca aggaagccgc cgtcttactg
    27361 atccttttgc ggggaacgcc ttatatttat tacggcgaag aaatcggtat gcgagagacc
    27421 aagcttaaaa aatccgagat gatggatccg gtcggggtta tgtgggcgcc atttaatcgg
    27481 ggacgggacg gttgtcgagc tcccatgcaa tggaataatc aaaccaatgc cgggtttacc
    27541 agcgggacac cttggaataa agttcatccg gattatccag agcggaatgt gaatgcgatg
    27601 gtccatgacc cgaattcgct gttgacgttt tacaggcaac tgatcggatt gcggaaacaa
    27661 ttccgctgtc ttcaggtcgg aaatttggaa cttttcgatc aaaaaaatga atccgtcctg
    27721 acatttacca gaaaatttgg gaaagaaatc gctctgattg cgattaattt cacccaattt
    27781 ccgcaagaaa cagattgcaa agagttgggt tccgagataa tttggacgaa aagtttttca
    27841 aataaatccg cggatatttc tcaaccgaaa gacggaattc tccggttggt cggggaacaa
    27901 gcggttttgt taatcggcaa ataaaagacg ggaaaccgaa tgatgaaaaa aacgaaatct
    27961 tggggagttt tactcttttt gacttttctg tttattgccg gcaggttgga tgcttacggc
    28021 attttgagag attcggtcgc tgcgaatgat acctcttctt attttgattg tgcaaaggca
    28081 gattttacga aaatcgaagc gtatgaatgt catcgttcgg ctaccctacc ttttattatt
    28141 caaaaactta atccggatct caaatatgaa ctgaccgacg tttcggagcc ttttttcgga
    28201 tccgaagcgg aattacaagt tcagcccggg acggagaagc tcgttatttt tcagaccctt
    28261 ttgtccattt tcagttggtg ttttttcact ctgaccgttg tcggattgtt ccgtcacgaa
    28321 ctgagcaaaa tcttcgcggc cattatttta ttcggattcg cttttgttcc gcaaatcgcc
    28381 gactgggaca gcatcctatt atccgaatcc atgtcttttt ctttttttat tctgctgtgt
    28441 gcgttcttaa tccggttctt acagaattgt tacggtcccg gaagtttaat gcgcagattt
    28501 ttatcgggct ttttcctgct gatctcggca attttttggc tatttacacg ggataccaat
    28561 gcctatttta tcgccttctt gtccgtgata tttttcatcc tgttttgggt taccgtattc
    28621 agccatcgta aattccaggg gttgatgttg gttttgtctc tgtgcttggc ggcttgtttt
    28681 ttgttccaac agcaaacctt tcgcaccagc aaacgatggc tgttaccgtt attaaataac
    28741 ctgacaacca atgtttttcc ctatccggag cgtgtcgcat ttttcgaaga aaagggaatg
    28801 ccggtcaatg aggctgttct ttctcaatcc ggttcggcag agtataacga tttatacaat
    28861 caaaaagaat ttattcaatg ggctgaaacg aacggtcttt cggcgtatac cgcgtttctg
    28921 atgaatatgc cgttatggag tgttcagcaa atctttttaa acttggatgg attttttgcc
    28981 gaaaatcttc agccgttttt ttacgggaaa ccggatgaaa agccccattg ggcggataag
    29041 gtcggaaatg ttttgcatcc gttatcttcc gccgtcctct ggatcgatct tttcctattg
    29101 ttgatattgt ttatttatac aattcgaaaa cgagatgccg attcacttgt atggtttttg
    29161 ttttcatttt tgttgacaat cggaagcatt atgatgattt cggttgctta tttaggcgag
    29221 gtgcgttccg tctggaggca tgttctttgc ggagtgatgc cgttgcgatt gattctttgg
    29281 atccaaattt tggaaatatt ggatcagtca cttgattccc ggccttctcc ccctatcatc
    29341 aaccgcctgg gaggacagtc gaatcgttcg gaaactgata aaattctgga tagataaaga
    29401 ataatttagg atattgacag gagtgaaata acaatgagaa gaccaatggt tgcaggcaac
    29461 tggaagatga ataaaaccgc tgcggaagca attatcctcg ttcaggagtt attacccgag
    29521 ttacagaaac aaaccgctat cgatcgtgta atatgcccgc cgtttaccgc tttatatccg
    29581 gtttccggac ttttgaaggg aagcggtatc ggatgcggtg ctcaggatgt gttctgggaa
    29641 gaaaagggag cctataccgg ggaaatagcg ccgaatatgg ttgccgaact gggtgagtat
    29701 gtaattatcg gacatagcga acgacggact tatttttatg aaacggatga gacagtcaat
    29761 aaaaagacac ttgcagcctt agcggccgga ttgacgccga ttgtctgtgt cggagaaaca
    29821 ttggatgaaa accaatccgg aaggacgaaa gaagtggttt cacgacaggt tcgcgaaggg
    29881 ttgaaaggaa ttcccgaagt gtatgtcgaa aaaatcgtga ttgcttatga accggtatgg
    29941 gcaatcggga cgggtttggc ggcaaccggg aaagacgcaa accgagtgat tgcggaagat
    30001 attcggaaac cgtatgccga tctttacgga gaaaaagcgg cacaatcgat tcgggtgctg
    30061 tatggcggat cggtaaaagg aaataacgcc gcagaatttt ttggacagcc cgatattgac
    30121 ggagctttgg ttggtggcgc cagtttgaaa gcgcctgatt ttatggaaat tgtaaacgct
    30181 gccgcgaaat aagtattcaa tcaaagtctg aaaagaaaac ttccgaaatt ttggaagttt
    30241 tcttttttat atcagtcata ccggggataa aatcaaggct gattcacgga gatcttggtt
    30301 tactcgagat gaagccaaaa tcgtctatgt taacggataa atttcattta ttttcaagaa
    30361 cagaatcact ttttattcga gaaaaaggtt gacattatga gaaaattgat gtatgatcac
    30421 cgacataacg agtaaaccat caatagatga cgtgagaaat atattttgag ccataaaggg
    30481 tcatcacttg tatattatta gaaattcgtc aatttcctct gtcgattcgg agaattaatg
    30541 aataaagttt cagagaaagc tctgcaacga ttaaacggaa gattaataga ttgtcaattt
    30601 aattggtgca ttcgcaccaa ttagcaatta attatcaaag cgcttgagag cgccaggagg
    30661 caagatatga atatcctcgt tgttgttggt ggtggggcgc tgattctttt tctcgcttat
    30721 ctcacctatg gtaagttcat ctccacgaaa gtgtttgaac ttgacaattc aagagtgacc
    30781 cctgccgttg agatggaaga cggagtggat tatgtaccga ccgatgccaa atatttggcg
    30841 ggtcagcact tttccgcgat tgcagccgcc ggtccggtta ccggaccgat cattgccggt
    30901 atcgcctatg gctggatgcc tacaatgtta tggatcctct tgggaagcat ttttatcggt
    30961 ggcgttcatg atatgggtgc tttggttgct tctatccggc acaaagcggc aggaatcgca
    31021 gataccatga gacaatatgt ttcaaaaaga gtctggattc ttttcaactt attcattttc
    31081 tttaccctgg tgatgattat tgtcgctttt accgatatta cgacttcctc ttttatcaac
    31141 acagttacgt tggatgacgg atccgttgtc ggaggcgggg caacggcaac ttcctcaatc
    31201 ttgtacttga tcctgccggt gattatggga tttttgctcc ggtacacaaa attgagcctg
    31261 ttgtgggcga ctatcatttt cttgccgctg gttgctttat ccatttgggt tggtcagtat
    31321 atcccgttga attatggcaa taaaaatgtc tggaacttaa tcgtcattct ttactgtatt
    31381 atcgcgggaa ttgttccggt ttgggcatta ttacagccgc gcggtcactt gggaggctat
    31441 ttcctttatg caactttggt tttcggcttc atcggaatca tattcggtgg tttcgatacc
    31501 caattcccgg cctggaccaa gacctatggg tcatctgatt tttggactcc aatgttcccg
    31561 atgctcttta ttacggtcgc atgcggtgcc tgttccggct ttcacagttt ggtgggttcc
    31621 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttattacg gtcgcatgcg
    31741 gtgcctgttc cggctttcac agtttggtgg gttccggaac tacatccaag caactggcaa
    31801 aagaatccga tgcaaaaccg attggttatg gaatgatgct cgctgaatcg gttgttgctc
    31861 taatggcgtt atcaaccgtc atgatcctta cgaaagatga tccgatttta actaaatctc
    31921 cgaacttcat ttacgcgaat ggtttgggtt cctttatgca gttactcgga atcaataaag
    31981 ccttcggtat ttccttcgga ttgatggctt tcacgacgtt tgtctatgat acattggatg
    32041 tctgtacccg cctcggtcgt ttcatttatc aggaactgag tggtatgaaa ggtctgatgg
    32101 gaagaataat cggtactgtt ttgattggcg gtattccgct gattctgatg tcgattaatt
    32161 tgacggatcc cgatggcaat aaagtggcaa tttggagctt gttctggaag acgttcggct
    32221 cttcgaatca gttactggct gctctggctt tggtgggtat caccgtttgg ttacaacgaa
    32281 ctgccaaaca tcggaaagcc tggatagcga ccgttattcc ggctgcgttt atgtttgtca
    32341 tgagcacttg ggctttagtt aatactttcc tgtcctatac atacgtcaag ggcgcctggg
    32401 tcatgccggt gggtcccaat aaagttgttc cgggattaag cttaatttat attattctgg
    32461 ctgtttgggt tgtgattgaa tgtgtaccgg caattgtcaa gaacgcccat tatcaagacc
    32521 ggcaaccggt tacacaggaa gcataatttc taaaattgtt ccgtatagat ttaccgcctg
    32581 agttttcgac tcgggcggta tttttttctt atatcatttc aatcgataaa attgtaagtt
    32641 tctgatagtt tttgccagcc tttttcgata aaataaaaac agaaccggaa gaacgccgtc
    32701 tcttttgaga cggcgttcgg attgaaagat gggtaaatgg taaagaaatt ttatcaattg
    32761 actgctaccg agcgaatcaa agaactgatt gatttaaagg tgatcactga agaagatgcg
    32821 gcgcttctat taaaaagggg ggggctctct tcggaagttg ccgatcacat gattgaaaac
    32881 ggaatcggta ttttcgattt acctttgggt gtcgcgcaaa attttatggt taacggccgc
    32941 tctgtttggg ttccgatggt gatcgaagaa tcctcggtta tcgcagcggc ttccaacggg
    33001 gcaaaaattg tgctgcagaa tggcgggttc acggcgaaag cagaccctcc ccggatgatc
    33061 ggacaaattc aggtgatcaa cgttcccgat ccggtgccgg caaagaggaa agttcttcgt
    33121 cacaaacagg ctttgatcgc gaaactgaat gaaaatcgcc cttctctggt aaacagaggg
    33181 ggtggcgttt tggatttatc cggcaaaata tttcgaaaat ccgcggtggg accgatgttg
    33241 gttttttatc tcacaatcga tgtgcgggat gccatgggag caaacctgat caattcggcg
    33301 ttggaagaga tcgctccgga tattgcgaaa cttaccgcgg gagatattcg gatgtgcatt
    33361 ttatcgaatt atgcggataa acggttggta catgcatcct gtacgattcg accggaaacg
    33421 cttgccaaag aagggcaggc aggcgaagaa gtggttgaac gaattttagc ggcgaatgcc
    33481 ttggcggaag tcgatccgta tcgtgcggta acgcaaaata aaggaataat gaacggtgtg
    33541 gatgccgtcg ttatcgcgac cgggaatgat tggcgcgcgg tagaagcggg agcgcatagt
    33601 tgggcttgca aaaaaggtaa aattcgaccg ttaagctctt ggacgaaaga ttccgacggt
    33661 tccttacacg gtgaattgga gatgcctttg gctgtcggaa ttgtcggcgg agctacccgc
    33721 gttcacccct tggctcaatt tagtatccgg atgatgaaaa ttcgaacatc ctgtgaattg
    33781 gctgaaatca ttgcctgtgt ggggttagcc cagaatttag ccgcgttacg tgctctttca
    33841 acggaaggta ttcaaaaagg tcatatggca cttcatgccc gccagattgc cataactgcc
    33901 ggagctcaag gcgcggatat cgatcgggtt gcggaaacga tgtgtaaaga gggaaatatc
    33961 gctgtggatc atgcaaagga gattttgaaa cgaaatgaaa ttactaattc ctgaaaatcc
    34021 ggttggaatt atcggttacg gtgcctatat tcctcgctat cgtattcggg catccgaaat
    34081 ctctcgggta tggcacggtg attcggaagg gacgccgatt aaagaaaaat ccgtcccggg
    34141 aatggatgaa gacaccatta ccatgtctat tgaagctgcc cgtaatgcgt tggcacgggc
    34201 aggtatcccg gcaaccgaat tacgggcagt atggatcggt tcggagtctc atccgtatgc
    34261 ggtcaaaccg agcggtacgg tggtggcgga agccatcggt gcggatgttt ccgttttggc
    34321 tgcggatttt gaatttgcct gcaaagccgg caccgaagcg atgattgccg gaatgggctt
    34381 ggtcggctcg ggtatgggaa agtacgctct ggcaatcgga atggatacgg ctcaaggaaa
    34441 acccggtgat gtcttggaat ataccgcggc agcgggcggt gcggcggtca ttttgggacc
    34501 ggcggatgaa gcattggcaa cgattgatgc cgcttattct ttcgtgaccg acacgccgga
    34561 tttttggcgg cgccaaagcg aaaaatatcc ggaacacact caacgtttta ccggtgaacc
    34621 ggcttatttt aatcatttgg agcattcgat tcaaaccttt tttgatgaaa cccatacgaa
    34681 agcttccgat tatcgttacg ctgtttttca tcagccgaac agtaaatttc ccgttaaact
    34741 tgccaaacaa ttcggcttta agaaagaaca atatgaaacg ggactcttgt ccccgatgat
    34801 cggtaatact tattcgggcg cggtcctgtt gggactttcg acgattttcg atatcgcgca
    34861 gcccggggat cgaattttgg ctgccagttt tggctcggga gcgggatccg atacgttttc
    34921 acttgtcgta accgatgcga ttttatccag aagaaacaaa gctccgagca cgcaaaagta
    34981 tatcgatcga agaaccgaaa tcgattacgc tatgtatgcc cgttatcgga atcagtattt
    35041 gatgaaataa agaggaagat tattttgaac agaattgatc acagacagcg acccgttatt
    35101 ttaggcaccg gaattttacc gattgggcaa cattgggaaa tatcgctccg gcaactgggc
    35161 ggccgagcgg ttcggatggc gcttcaggat gccggaaatc ccaagccgga tgcgatttat
    35221 atcgggaacg gattgggaac cattctttcc catcaggcta atttgggtgc tttgattccc
    35281 gaatttgccg gttatcccgg gattgaaagc tgcacttttg aggcagccgg cgcttccggt
    35341 gcagccgctt ttcaagccgc ctgttttgcg gtcgaatccg gttatgtgga cgttgcggtt
    35401 gcattggggg tggaaaagat tacggaatgc gaggatcgga cagcggaagc ggcgatgaat
    35461 cttgcgttga attacgaatt tgaaagcagt caagggctga cggtaaagac cgaggcggca
    35521 ctgattgccc aacgatatct ctatgaatgg catctgaaac gcgagatctt tcaaaatgtc
    35581 gcactcaatt cctatcaaaa tgcccgagga aacctcaacg catttgctcg aaatatcgat
    35641 gaaaaagcat accaaaaaca accgatgatc tctggacctt tgggccttta tgatatcgcc
    35701 gcgcaaaatg acggcgcagc ggcggttgta ttgactcgag aagaattggt tccggcggaa
    35761 gaacaggatc gattgataat cgtttccgga agcggaaacg ccatcgcatc gttggcactt
    35821 cacgatcgcc atgactttct ccattggtcg gctgccggtc aatccgcggc cactgcatta
    35881 agacaagcgg acctgtatct ttccgatatc gacatatttg aaatttggga tggcaccgtc
    35941 atggatgtca ttttatctct tgaatcaatc ggcatctgtc aagaagggaa aggttggcaa
    36001 atcgattcat tgccactgaa tgttacgggc gggtgtttgg gacgcggaaa tccgctcgga
    36061 gcttccggca tgtaccagat tgccgaggtg gctcgatttt tacaggaaca aaaattggag
    36121 aacggtattc atcggaatgc tttgatccaa gcgatcggcg gatatggcgc aaccgctgtc
    36181 acacacattt tacggtcata aaagaactat caaaaactat catgatagtt tttgtttttc
    36241 ttttttactt atactactga acgttgtatg cggaaggagc aatcgaaatt ccttccgata
    36301 tgaagtctga aatcttttga aaacagataa taaagatttc aaaaatgagg agaaaaaatg
    36361 gaatcaccac gtcattggcg tttgaagaaa cagagatatg ccctaatcgg agaggaatgc
    36421 ccccattgta aggcaaaaat attcccgcca cgggatgtct gcccggaatg cggtttggaa
    36481 gcaaaaacag cctaccaatt cagcgggaaa ggaaaggtat actcatatac cacgatcaat
    36541 gaagcaccct ccggctttca ggatatgtct ccctatgcaa ttgcattggt taaattggaa
    36601 gaaggaccga ttgttacagc ccaattgact gatttgggcg atgaatccgt ttgggtcgga
    36661 atgcctgttg agatggttac ccggaagctc cgtaacgacg gcgatgaacg gggagttatc
    36721 ctatacggat acaaatttcg accggcgttt caagcctgat aaaatcaaac attcaattag
    36781 gaaagtcagc gttttcgtca atattcaacg gaggcgctga ttttttaatt taaagaaaaa
    36841 atatcgatca ggttgttttt tcccttttat tcttcgtata aagccattaa aggattagaa
    36901 tttctgcttg aaattgatca ggatcaaatt tttgaattac gaaaaaagta taatgaataa
    36961 aagtctcggt tttatgaata aaaattttaa gaatttagat cggaggtgtt atgaatgtat
    37021 caaaacgatt ggatgaagcc agaaaagcat atgatgaaaa aaatccggaa gccagcaaaa
    37081 aaatccatga tgaaaaagtc atcgagatgg ctgtcgaaca acacggttcc gaatcttccc
    37141 aattcatcgg gaacatggtt tatggagggt tggatgggat tatctcggtt tttgcggtag
    37201 tcagcggggt tgcgggagcc agcctgactt ccggtgtatt gttgattctg ggtgcggcta
    37261 atttgctcgg tgacggaatt tcgatgggga tcggcgctta tctttcgatg aagagcgaaa
    37321 aagaatatta tcgaaaagag ctggagcgcg aaagttggga agccgaacat tatccggaag
    37381 gagaaaaagc ggagttactg gcaatttata aaaataaggg ttttccgaag gaagatgcag
    37441 agaaattagt ggaaattcaa tctcgcgatc atgatcgctg ggtagatgcg atgatgataa
    37501 atgaattggg gatgattaaa gatgaacgga agccgaccag cgcagctttg atgacattcg
    37561 gttcatttgt ccttttcggt ttgttaccgc ttctggttta tttggtggat acgatttttc
    37621 atacgaattt gtccatgtcc accatgtttc tgatttcaat tattttgtcc ggcgcttcgc
    37681 ttttcggttt aggagcggca aaatatttta taactcgccg gaatccgctc gtcagcggtt
    37741 tggaaatgct gatagtcgga ggcttggctg cttccgtggc ttattttatc ggcgttttac
    37801 tcaaaccttt gggcggataa tttttgcctg tgcatgatat ctctcataga atagatagct
    37861 atcaattcgg ttagaaccaa tagctatccg gatcggaaac agacaaactt ctatcgaaga
    37921 ctgataaaaa atatattgct ctgattcaat ctgatttgcc cggataccat tttgcatccg
    37981 ggcgtttttc ttcattatcc gacaggataa gggaatgagg aaagggagac gacgtgagcg
    38041 gtaatgatta aaaccaatac aagtgccagc atgtaccaca tggcatgata ttttctgatt
    38101 gtctttttga attggggagc cgctttggta aagcgcatgg taaagccggc gaataccatc
    38161 catgccatta caatgatcag aatccaatta aagacggttg cgtattgcca taatgtaatg
    38221 gcatgaataa caattacgac aaaagcggca atattcccaa aataatgaac ataatgccat
    38281 ttattcctta gattattcag ccatttccat tcggcttttg gagcgaatat tctttgaatg
    38341 agatgcaaga tggtgtagat atttgccaga aaaaataaag ctcctcccaa accacccaaa
    38401 tttagaccca atgcgatatt gcccggtatt tcctgattca taatcttcac ctccgttaat
    38461 taataattat ttattaatca tttttcagac atgcttgatt taatgccagc atatctgaaa
    38521 tcccttatta aacgacccgt aaaaaattta atcaaaagaa tcaatttcat ttcaaaattt
    38581 catgaaattt gactctgatg taataagata cagagatccg ataaaaaaat caacaaatta
    38641 tcgtcaaatc aaaaaaagag tttgtcacta aaccattaga tttgtcattt tttgatattt
    38701 aaagtttata gtctaatcta tcggtctgaa cttatctttg gaagatccgc aaaccgggca
    38761 aacccagtca tccggaaggt cttcgaaagc tgtccccggt ttaattcccg attcgggatc
    38821 tccttttgca gggtcataaa tataaccgca tacgctgcat ttatattttt tcatttttgt
    38881 atgctcctta atgactaata tccatatttc ttttctattt ttagaaagtc cttataaaag
    38941 gaataatttt taaaatccaa taaatctatc agaatgagtc taaataaaca aaatactttt
    39001 aaatgagcga ttactcgatc gtttttgttt gaaaaaactt tgacaaataa aattatacaa
    39061 ccttatctta aaagaatgag agatactttg taattgagtc aggtttttga gaactatcgg
    39121 gctaatgaat ggcttaaaaa aagaaacaaa ttaagatgat taatcaggct cttaaatact
    39181 attattcatt gaaaaatatg gcatgataat tagaaatgta gagaaattag acaagggata
    39241 aatcatattg cggatgaaca tttttaaaca tggaggttta tgatggactt gatagaaatg
    39301 gtaatttatt ccgattatat ctgcccctgg tgctat
//
LOCUS       DAOSMM010000015        37944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1120_length_37944_cov_18.393307, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000015 DAOSMM010000000
VERSION     DAOSMM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 37944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37944
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_37944_cov_18.393307"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1530)
                     /locus_tag="PK898_03915"
     CDS             complement(85..1530)
                     /locus_tag="PK898_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19210.1"
                     /translation="MKKNRFFLWGIVLFLGILMLPVMTVSAKDKVVFGSDYVLESGDK
                     LNGNLVVFRGDVTIEEDAFVDGDMAIFGGDIVIDGEVSGNIAAFGGDISLGPNSIIDG
                     NFSAMGGTVTKSPTAIIKGDLLRQNGEILLNTDKRVEKNVFSPVARGNRNKTGFWLTF
                     FGVLGSIGLFFAMVLIIAGLGLLANVLLGSHLEGIGKIIAEKPWNAFGIGLVTVIAAP
                     FVVVLFCMTVILIPLAFLLILALILLIFYGWIACGYEAGQWMTKAMKVTWTSNWTTAF
                     GTFVITLVAHGLKKIIPCVGWMPGGIAALFGLGGVMIYYYHRYQDRPKKSTSNPQLPK
                     NNPPSGDYRPRENTIPQSSSEEEKMRQSREEPVAEKSEKGPNDSQKPESVIELPGKVE
                     TGETEEGSAQNGIEEMRGAEPVSEFDASEPQKETGESSAYAETTPKKKKRGVGASVLD
                     SIHESEMKDHSTSEDHPQTSNETDKGDSKES"
     gene            complement(1527..2057)
                     /locus_tag="PK898_03920"
     CDS             complement(1527..2057)
                     /locus_tag="PK898_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOU19211.1"
                     /translation="MDSHEYYEELILKDGDLSGEERKNLKAHLEECEKCQKLEEQWKS
                     CLNLLSKAPMVKPKADITGKWEQILQVRTFEKEMKNQNRQIIGTIILITVLSIFSCTV
                     LLSPEFLIPSTIQASQILLLLAGLANQFRTILSMLRLPVLIFSLGGILAVICFVVINR
                     IGINNEENSNEGVEQS"
     gene            complement(2063..2623)
                     /locus_tag="PK898_03925"
     CDS             complement(2063..2623)
                     /locus_tag="PK898_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU19212.1"
                     /translation="MNNISTDILEKVKEGDADAFTDIVDAYQNAIYNLCYRMLYNRTE
                     AEDATQETFWKAWTNFSKYDFNRSFSTWILSIAAHYCIDLQRKKKVPEIEIDETMEEI
                     IPDPIPHPEQEAIRNEEEQQIQDMLNSLNETDRAAIVLRYWYDFSDREIAESLSVSES
                     AVKSRLFRARKQLAQYWDGQTQFNQG"
     gene            complement(2696..3748)
                     /gene="selD"
                     /locus_tag="PK898_03930"
     CDS             complement(2696..3748)
                     /gene="selD"
                     /locus_tag="PK898_03930"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOU19213.1"
                     /translation="MSEEKKAIKLTELAFCAGCAAKLRAEVLMDVINPFGGLFNQNEF
                     PQLLIGLEEADDAAVYKLNDEQALVFTTDFFPPIVDNPYDYGAIAAANSISDIYAMGG
                     KPLMALNITDMPTNLPVEVIADIFRGGAEKAKEAGCPIVGGHTVRDQEPKYGLAVVGT
                     VHPDRILYKGKLKVGDKMVLTKPIGFGLMTTANKNGSADSEDFQKTVDWMKKLNKTAS
                     ELAVSVGCHSGTDVTGFSLMGHSWEMIRSGKIGLRYDWDAIPKIDHYEKYMNMWLFPG
                     GATDNRNYYGDKVTFKIALSEQQQMMLFDPQTSGGLLLGIGSDRLDEFLEKAKKSGQS
                     AWVIGEAVEKPGIEII"
     gene            3904..4662
                     /gene="yqeC"
                     /locus_tag="PK898_03935"
     CDS             3904..4662
                     /gene="yqeC"
                     /locus_tag="PK898_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HOU19214.1"
                     /translation="MNSFESVTERSILSIVGAGGKTSLLFYLAEVLPWNCLVTTTTKV
                     GRDQISSADRIVSFSEFLAAPDHFSGDKIVWVSPAPEQDYPKVKGFDPAQFSEFAEAA
                     RKIRMPILVEADGAHRLHLKAPAENEPVVPAESDHILDVVGLDVIGLAIGERTVHRLS
                     QFLRITGCREGDIIDESVICRAILHPQGGFKNAPQGCCKIAVLNQADTAERIKHAENI
                     SNYLLKHGVDEVWITSLDPETDKNKRGIIKELKK"
     gene            4659..5489
                     /gene="yqeB"
                     /locus_tag="PK898_03940"
     CDS             4659..5489
                     /gene="yqeB"
                     /locus_tag="PK898_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HOU19215.1"
                     /translation="MNQKNCHPREITAFRGAGDLASGIALRLWRSGYRVILSELPCPL
                     CIRRSVAFANAVYESHMAVEGIESILIHDIAEAETIWAEDKIPLLIDPDISQIKELNP
                     SVIIDARIMKSYRDDSHLSDAPLVIGLGPGFVAGENAHAVIETNRGHDLGRVFWKGSA
                     EPDTGTPGMIGGEGPKRVQKAPASGIFTPKVEIGDFVKEGDLLALVGDTEIRSPLTGM
                     VRGVIYPGIFVNKGLKIMDIDPRGERRYCFTASDKANALGGSVLEAILTWNVRESARQ
                     "
     gene            complement(5449..6483)
                     /locus_tag="PK898_03945"
     CDS             complement(5449..6483)
                     /locus_tag="PK898_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOU19216.1"
                     /translation="MREILQAIVDAHAGREDLVMATIIDNVGSSPRSAGTKMLIKPDL
                     SIIGTIGGGKLEANAILAAKEVFQSKKSNLFHFILNGEDAAKSDMICGGSGDVLLVFL
                     PWDDPETTLVFEKALDAAVGNQEGWLITQFRENGVDTNKFCFVDRQGEKTGNLIINGE
                     FLSDMRGTPDQIAIHSDLLLNSEILVEKIKERTKLYIFGGGHVAKETARLADEVSFET
                     IVLDDRAEFANKERFPNSKIMVVPDYKSLPDLPIDHQSFVVILTRGHLGDYDVLKEML
                     KTDAFYIGMIGSIHKRNLIYERLLNEGVSQKEIDRVHSPIGLPINGETPTEIAISIIA
                     ELILERSKLK"
     gene            complement(6524..7075)
                     /locus_tag="PK898_03950"
     CDS             complement(6524..7075)
                     /locus_tag="PK898_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOU19217.1"
                     /translation="MSRKQDPLSVEFLLLGIIRKQPIHAYDLDKMLKEKEEFSVVWRF
                     NQSQLYAILDKLEKNNLIQSEITQGSAFPFRKIYTLTQEGEDAFQAWKVRPVEKPREL
                     RSDFLVKLYFLKDEPYQIFESVIKQQIMLCNQWIELQNTHLKNQPDDAVYLRMVYQFR
                     LESLRSNVTWLNECLSIRKNEED"
     gene            7326..7514
                     /gene="rpmB"
                     /locus_tag="PK898_03955"
     CDS             7326..7514
                     /gene="rpmB"
                     /locus_tag="PK898_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOU19218.1"
                     /translation="MAKCEICGKTTVFGHNRSFSQRATNRAFYPNLQRVTVYENGQRV
                     QKTLCTKCIRTMAKLPKE"
     gene            complement(7583..8248)
                     /gene="rpe"
                     /locus_tag="PK898_03960"
     CDS             complement(7583..8248)
                     /gene="rpe"
                     /locus_tag="PK898_03960"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU19219.1"
                     /translation="MQRNEIIVSASILASDFMRMGEEVQAVAASGTDWIHVDVMDGVF
                     VPNLTLGFPFIEGIRKITTLPLDVHLMIIQPEKHIERFIEAGADILTVHCENNPNIHS
                     TLLNIRKMGASPAIALNPGTPAEMVEPLLPFLDMVLVMTVNPGFGGQEFIPEMLEKMR
                     KIRKWIDEKQLNVRIQVDGGINHETAGQCFAAGADTFVAGTSIFRNSNGYAAGIRELK
                     NLC"
     gene            complement(8264..9091)
                     /locus_tag="PK898_03965"
     CDS             complement(8264..9091)
                     /locus_tag="PK898_03965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU19220.1"
                     /translation="MKTDSDEISQFHIIRMLANGVTTVIYEATDQKRERKVALKVLND
                     EHREDRYQQQKIIHEAYLQSRIRDPHVVQVYDLIDRENSPCMVMEYVEGENLWQTYHR
                     KPEFTDSAKQSIALQLVSGVRSIHAMDIVHCDLKPLNLLLSSGGNLKIIDFGEAFALE
                     STEDSGWQKPEVVWASPLYMSPEQCRGEQPVKATDIYSIGIILYQLYTGQLPFYSEDP
                     EEMLDFHCNRMPQPPHEIADLPDDIDRIIRECIRKNPQERYRDAGELFIALTKAYHQ"
     gene            9137..9649
                     /locus_tag="PK898_03970"
     CDS             9137..9649
                     /locus_tag="PK898_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19221.1"
                     /translation="MADNHKNRIFNLSTGLISLCVAAFIFLGIILLIVNIGPSPAEEK
                     EDALAQLTVIPMPTITPTSAIPSEKPDLSVKYISPEGFSVGAYVKIENTQGAGLKIRP
                     DPGTGGEVSFIASEGDIFIIIDGPDERNGYTWWKLQGFEDPSLVGWGASKFFSLVAPA
                     ADQIQSSDIQ"
     gene            9649..10431
                     /locus_tag="PK898_03975"
     CDS             9649..10431
                     /locus_tag="PK898_03975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOU19222.1"
                     /translation="MSHNQSIHALESANAHSIGKIRRYNEDALVSFQLETMRKDQTDS
                     LGLYVVADGMGGHINGDEASASAVSAFTEQLGNDIIPLFLKDESDSSIIPELLKKAVL
                     FANQSILDAHPGSGTTFTAALIWNEKIYIAHVGDSRLFLIHQNYEMEQLTHDHSLVQM
                     MVDLGEISEEEALNHPRRSVLLRSLGFDKDIEVDLAENKLEAGESLLLCCDGLWSVIK
                     NDQMIKIIREHNDIQEAADALVDAANEAGGPDNISCILVRRK"
     gene            10507..11295
                     /locus_tag="PK898_03980"
     CDS             10507..11295
                     /locus_tag="PK898_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU19223.1"
                     /translation="MAEGNKRYLILVVDDDERIVRMIRLNLEHDGYSVIEANSGMEAI
                     NQVREKLPHLVILDVMMPGMDGFETLSLIRETSQVPVIMLTAKGEEEDRIRGLELGAD
                     DYITKPFSPRELMSRIKAVLRRTEGTSGMDTGEVIEVDEHLKLDFGKREVWLDGQLVK
                     LRPTEYRLLYHLVQNAGWVMTYDQILAKVWGYEYRDETHYVRLYINYLRQKLEKDPSN
                     PKYILTERGIGYRFVDYKKGKLKEGEGETKENPNNPEESHPSDN"
     gene            11437..11931
                     /locus_tag="PK898_03985"
     CDS             11437..11931
                     /locus_tag="PK898_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU19224.1"
                     /translation="MYGKLRVQPVRDLFTMQDAMDRLFNEFFGDSDNLQTEVLAPRVD
                     LKQKENEIDVKLSIPGVDPDKIQISVADNVLTVKAEMEEEKEEKDEKAVYEIREHRWT
                     SYYREIALPCEVEADKAEAEYENGVLKIKLPKAEIVKPKLISISAGKKGEKSTEKIES
                     KTGK"
     gene            12038..12604
                     /locus_tag="PK898_03990"
     CDS             12038..12604
                     /locus_tag="PK898_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19225.1"
                     /translation="MQLTDTSLVLIAIMPKLRDMEIARVLGWYRIPMKTAPKILLTDY
                     IAFYQTKDFGEEHKSRIEKYAEVRGVELTTRQELFRDEPDHPRAKEEYYKIQIGPLQA
                     LENPILADRWKRFLFFYTSGEKMFTASTLHDLAITGSEREILWKSLRERRENDPEFFR
                     STEKKEEIPEQLMFLLGNLTLSNLDFNE"
     gene            12655..14070
                     /gene="hydA"
                     /locus_tag="PK898_03995"
     CDS             12655..14070
                     /gene="hydA"
                     /locus_tag="PK898_03995"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOU19226.1"
                     /translation="MEKIIKNGKLVTDSEIFDADILIRDEKIVAVGKEFNESNAEIID
                     ASGKLVLPGGVDAHVHLDLPMPGTVSSDDHYTGTKAAAFGGTTTVIDFANHDLPSLID
                     SFNVWQEKALPNVAIDYGIHQNFTRFNENTLKEIPLLPKMGVTSIKMFTAYNGRMRLQ
                     DGEIFQAMRVAKDEGIISLVHAENGDLIDLLVAEALAAGHTAPVWQARTRPAWGAVES
                     GFRVAALSFSAGKAPLYIVHMNVKEEADMLLYARENGLPIFGETCPQYLLFTEKNLEQ
                     PDGAKWVCSPPMRSEKDNEGLWEALADGIIDVVSTDHCPFLFDGTKPILYEGKPYQQP
                     GKELGKDDFSKIPCGLPGVGDRMPVLWTKGVNEGRISPTRFVQLTCTNPAKIFGIYPQ
                     KGALLPGSDADLAIWDPNKIVSYGVKIAKHRTDYNLYEGTELKGFPEKVFLRGSLIVD
                     GDNWLGKRGGGHFLKRSRFLG"
     gene            14078..15655
                     /locus_tag="PK898_04000"
     CDS             14078..15655
                     /locus_tag="PK898_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19227.1"
                     /translation="MRFLTVPIGILSFAAILIFLITRLRKKIGVAWFIGALASFSVWC
                     WIFYLKRTPESVFSIRWQNLELGFLLDNISWSYMAASATMLFFMMASAPSRLNPDSSS
                     NDWGFFLLITAIVMATVASDSGWSMVFGWFLFDLLTVGIQFFSHQKIYPFESLEKILF
                     IRIFSIFLAAVAIAIGNSIELNPAQSFLEGQRETILLIACAMRAGLFPFFEPFQNENE
                     TRKGLNTFVELSSVALVLPAFIRIDSSAMGNDAYGFFMFFSTLSVICGGLGMIFSNKN
                     ASERRYFVLTLTGLSFISALRFQPTASVVWGISLSVLTAPLFLYDIRSRLLNVVQVIS
                     GLLLSGIPRTPAASGWRGLVLVPPSFFDFFLMISFACLLTKFFSDLTVQNPAHPALQD
                     RRMNAIYPFGFMVSVIAYGVIAALSWNFYSDNGMIPASIITLIIAFIIGFRSVWLRFF
                     TQMNTFFSWFREIFFTIWKIFLWIIQFRWLEAPIRWIITGLSAVIDFISGIFEAPGGI
                     LWKFLILIIVLFLVIGI"
     gene            15664..16266
                     /locus_tag="PK898_04005"
     CDS             15664..16266
                     /locus_tag="PK898_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19228.1"
                     /translation="MRGLFFTSIIGGALIILGALIIFIRLNQPDSRISLIAYILESVG
                     IVLLLVRIFSPQILISVFLTGTMGAAILGTDQVALHIPLKPDKVNPQGIFRLLLGFIL
                     AVLILSIEPEIAVWIPVPDSILFCSLFLILMGLTTFSLSSRLMYRFLGILNIFSGFQL
                     VYMLLEESVLVFGFMIAIDLLIALLGAYFMSGSQNDEYPT"
     gene            16288..17685
                     /locus_tag="PK898_04010"
     CDS             16288..17685
                     /locus_tag="PK898_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU19229.1"
                     /translation="MNAPLLWFIIPFFFGAFLLLFSESKRLSIILASIVCLFLIAISA
                     TVPINSMVIIGKNSIIFTDSMEILGRTLSLERAAQPLLAFFYSYAFIWILFSAAVRVH
                     RFFVPLVLMETALVIGVTAVSPFIYGAFFVQLAVLIALPIFWNEKMKSGEGIIRFLLY
                     QTLGLIFLAIGGWLASAVELNPSDQYILKRTMVVLLVGFIFWLAIFPFHSWIAILMDE
                     SCPYLSGFVINILQFSSFLILLYFLKSYLWLRTYDPLFIGLRMIGAIMLIIGSLWAVF
                     QKSVQRLQAFLIVAENGIALFLLGLHTTQSFSVFISLLFIRMIASFFWALAVKSLPEN
                     FDFGLNSLQGLYRSDPFISLTLIVSYFSTVGLPFLPGFPYQATFLQLAFSESSSMGTV
                     LSIGYWLLIAAGFRLAVTLLHPAEKAAGKAETANQRALLIIGIAALFLITFFPEPFNR
                     FIAQIQSQFTLISGG"
     gene            17738..18946
                     /locus_tag="PK898_04015"
     CDS             17738..18946
                     /locus_tag="PK898_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19230.1"
                     /translation="MAKTWNGGPLNEGFVDLDQLVKRLEWLDNERRNDKNRILAIEER
                     LTDVVSENVLLKNQIKDLEEEIQRYANIQAKIEQNESLIAQLRIDAFRAIEEKDKAHN
                     EREKEIDAARRNGIDSINRTLADLRKSIEPLQDIRKTLLSRNEDSIRLGRMIEELQAK
                     IDAVAMDKDEYARFQKSVEENRRQDAKRVTDIQAEVTAYRKRIEDMRSKFDLVSEGLR
                     KVEQRMSELLAAENERKQSVTDFMEKQSLANLEYEKTWQQWKTAFDDIVAKAAGLDTQ
                     IETLENLQRSVKRSQDTLDEVTQRFDRRTNELVEMQRLMEEKLRQEWANFKADNQKRW
                     TNYSLGQEEIQAEYRRNIEALQARMQAIEDASASALDDVNLRDELYISQLQDLFTMVR
                     GWLEKMGKAS"
     gene            18985..20295
                     /locus_tag="PK898_04020"
     CDS             18985..20295
                     /locus_tag="PK898_04020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOU19231.1"
                     /translation="MSQITVIGTGYVGLVTGACFSDLGNNVICLDIDEKRISDLNHGI
                     MPIFEPGLEEIVERSHKAGRLQFTVNYPEALRDAEFVFIAVGTPSGSDGEADMSYVEQ
                     AVIQIADNMDHPIIIVDKSTVPVGTGDWVADIIQKYRDGHMPKFQVVSNPEFLREGSA
                     VADFMHPERIVLGSNDTEACDRVAELYAPLRAPMLVTDIRTAEMIKYASNAFLATKIS
                     FINEIANICESVGANVIDVARGMGLDNRIGSKFLNAGIGWGGSCFPKDVKALAHIAVE
                     YGTQPQLLQAVIDINQTQRRRVVEKLNRMLNGLEGKEIGILGLAFKPDTDDMRESPAA
                     DIIRYLTNEKAIIRAYDPEAMEQAKKILPKEVILCDNPYQVVENADALLLATEWNEFK
                     QLDFKKIHDLMREKNILDGRNLWDPERLSELGFTYVGVGLGKYI"
     gene            complement(20409..21698)
                     /gene="tyrS"
                     /locus_tag="PK898_04025"
     CDS             complement(20409..21698)
                     /gene="tyrS"
                     /locus_tag="PK898_04025"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOU19232.1"
                     /translation="MSEQHSIYGELKWREMIYDSMDGLEELLEKEKVTLYNGFDPTAD
                     SLHVGHLVPMLALARFQRYGHHVIALAGEGTGMIGDPSGRSDERNLLSAEEAHYNVEK
                     IKRQLAKFLDFEIKSNPARIMNNGDWLLDLKLIDFLRDTGKYFTVNSMMAKDSVKSRL
                     SRESGLSFTEFSYSLLQAYDFYHLFVTENCVLQTGGSDQWGNITAGCELIRRKTGKNA
                     YGLTYPLVKKKDGTKFGKTAGGAVWLDPERTSPYKFYQFWLNTDDADVIPFLKYFTFF
                     DRQKIDELEFATRENPEGRAAQKALAVEMTKMMHGETALDKAMQASEALFGGEINGLS
                     AADIKEIFADVPSGVIAADSMNGEGLPIVNLLSDCGFMKSKGEVKRAIAEGGVYINNH
                     RVTDVNTSIRKDQFIEGKYLVLRRGKKNYFLLKTEQE"
     gene            complement(21739..22173)
                     /locus_tag="PK898_04030"
     CDS             complement(21739..22173)
                     /locus_tag="PK898_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU19233.1"
                     /translation="MEIVEIKEMSQAVLDAFTVLVPQLSSSAKIPTWDELEDLIQSRA
                     SILLAAVDPDQNNKIVGSLTLAVFRLPTGVRSWIEDVVVDEQMRGRGVGEMLVRAAVD
                     RARAEGAKTVDLTSRPSREAANRLYRRCGFEPRETNIYRFTI"
     gene            22415..24283
                     /locus_tag="PK898_04035"
     CDS             22415..24283
                     /locus_tag="PK898_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19234.1"
                     /translation="MRQKSYKKDRRNGRGFSVCILILALICLIPIAGGYEFTLSAGEF
                     LAPDGTVESFTPEVYSWLRAVFSVLFLVLMGLFITSVCIPERFFEFWREQWRSFKGFP
                     SALKSDTGTFFSAVRQFFRENRKDIPLLLILFLSGMAIRLWIINRPITHDEAYTITTW
                     GSGSLKYAVSDYQLPNNHVFHTILVNLIYHTLGKTPALLRLPALINGLLLIPAVYILG
                     KQIYNSATGLLAAGISAFSSFLIDYSVWARGYSILAFCAVLIFIEGTIVQKQKNRFVW
                     CLLILTAVIGFYTLPVMLYPFGAFCVWFLLRALFYRKFPLSGYGSMANALKYFIAAGI
                     VIVILTGLCYLPILRNSGIHALFGNVFVRPLPLADFKPTLISRAIDFYRAFTDTVPPV
                     IRIALFLGNVLALIFHRKISRIFIPLQAAFLLWLLPLWAIQRPNLWPRTLIFLWPFLI
                     LWGSAGVIWIVEKICAFLPSGLQTRFIPLFIIVAAGVTAVPQFDRAAEIAKLSSPHET
                     AVLRILENRDTEKILFVVAPEDDAPLWYYADRYGLPKSIFDKNIPFSTVYVYVNPKNE
                     GFEEPRTLDEIINRYGPGESFLDRSTEKVLMASENAILYRFHANERVIRKTFGETP"
     gene            complement(24294..25133)
                     /locus_tag="PK898_04040"
     CDS             complement(24294..25133)
                     /locus_tag="PK898_04040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU19235.1"
                     /translation="MEERIQKILSRAGYGSRRDCEVLIKEGRVTRNGEKVELGAKADP
                     VKDQLAVDGVTLKFKEPEKRYIALYKPRFVLSDRSASDPRRTVFSLVPNSDDLFVVGR
                     LDFESEGLVLLTNDGELANKLTHPRYEKEKEYRVLVAKRPDEDQLNKWRRGVVLEDGV
                     KTAPADVRVIRQQGEGAWIRVVMKEGRKREIREIGKQIGLPIVKLVRVRIGSLTLGPL
                     KPGEWVALKDEEVQALKESASAKPAKKPEKKLFRREIAGSRMKSGAPALSKGQPKQTK
                     RKS"
     gene            complement(25182..25748)
                     /gene="scpB"
                     /locus_tag="PK898_04045"
     CDS             complement(25182..25748)
                     /gene="scpB"
                     /locus_tag="PK898_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOU19236.1"
                     /translation="MTENSTFDDKEPMTILESILFVASGPVPFSQLAMVTEMKPSEVK
                     ELVRKLAGYLEAENRGIRILVHDDKVQLTTAPETADFVEKFLGIESAQKLTRAAQETL
                     TIIAYKQPVSRPAIDAIRGVNSESVVRGLLSRGLIEEVARAETPGRPVLYATTADFLQ
                     YFGLSSIEELPPFEETIEETDHKQILKD"
     gene            25792..26367
                     /locus_tag="PK898_04050"
     CDS             25792..26367
                     /locus_tag="PK898_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19237.1"
                     /translation="MNKHKFILPVIFLLCIFSAGWYAYSPTSQNDSGATALETLETEG
                     NRIANLAAGTPFNLTVSDDTLTSAAEELMRQYKNEIETAIKDAAGVKLSLSNPKVEFT
                     NDGFNLSVKAGLGFLKLKAKINGKVSLVDGKPQVVVEKIDVPIIDISADTVNNEIKSL
                     SDQYMNQLTDSYSLSKIELTEGQAVVEGIKK"
     gene            26444..28015
                     /locus_tag="PK898_04055"
     CDS             26444..28015
                     /locus_tag="PK898_04055"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HOU19238.1"
                     /translation="MFFSFLLIFSANACQTVDFLNRTGSAEKITVNVEKGGNLLTGTC
                     AAGSTVEQALQELKVKYQPEDIITPVLSTVLSEDTTVKITSVTFEEEQKEQPIPFKSL
                     TVQNESIPEGETYIIQAGQNGSEKITIKSTYEDGVLVSQVTSERNVIRDAVPEILMYG
                     VKAEHAPIQIPGKIVYISNGSAWLLENTTGNRIPIVTSGDLDGRVLDLSSDGEWLLYS
                     RKTQNDAINSLWMLHITDWNAKPIDLRISNVIHFAAWLPGEARRLLYSTVTPQESAPG
                     WKANNDLLFRLVSDTGMLMEDETIVQANDEGPYSWWGTEFYLSKDGRTLLYATPGSIG
                     KIDRLTGEKKELTKIQPYEKTRSDWAWIPGVTWSSDETAAFFAFHGEIEGTTKSYDPT
                     DFNIGKINLTDGQAAEIKSGTGLFSQPAASPLFEDGSSYIAYMQTISPLQSESSRYRI
                     MLMDSDGGNAKAVFPLDEEDYVTPQRVIWSPTTVANQSWIAIIYKSNIWLVNPFTGIY
                     NQITIDQSVTKMIWK"
     gene            28038..28997
                     /locus_tag="PK898_04060"
     CDS             28038..28997
                     /locus_tag="PK898_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU19239.1"
                     /translation="MRILITGAAGFLGSHLCDRFLREGHEVIGMDNFITGNPQNLAHL
                     AADQNFSFIRHDVSNFIFVPKKVDAVLHFASPASPNPLSPYGYTNLPIQTMKAGALGT
                     LNALGVAKANNARFLLASTSEIYGMPLEHPQKESYFGNVDTIGIRSVYDEAKRFAESL
                     TMAYYRFHGVDTRIVRIFNTYGPRMHLDDGRVVPNFIQQALRKEQLTVYGDGQQTRSF
                     CYVDDLIEGIYRLFLSDEHYPVNIGNPVENTMVELAEEINRLTGNPAGLIYKPDLRVG
                     DDPQRRQPDITRAREILHWEPKVSLEEGLMRTIPYFEQAMKHD"
     gene            28990..29451
                     /locus_tag="PK898_04065"
     CDS             28990..29451
                     /locus_tag="PK898_04065"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU19240.1"
                     /translation="MIDFINNPKERSRFLKFCTVGIIGAVVDFGVMNLILYYTGANSR
                     IASTISFIAAVISNFFLNYFWAYRDSREKSISTKLFQFFLVSIIGLGIRYLLFLTIEN
                     PIIGFCDRILPADFFMSVETAGHNLTLVISILIVLIWNFFANRFWTYNDVS"
     gene            29458..30036
                     /gene="rsmD"
                     /locus_tag="PK898_04070"
     CDS             29458..30036
                     /gene="rsmD"
                     /locus_tag="PK898_04070"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOU19241.1"
                     /translation="MATPRIIAGTAKGMRLHSVPGDSTRPVTDRVKEALFNILGTDIA
                     DAGMFDLFGGTGSVGLEALSRGASYVRFMDANRNAYKTLQENIKLTGFEKKSKAIFGD
                     VFGYLRTVPDREFEYIYVAPPQYKRLWQKTMQILDDNDGWLSEDGWIIVQIHPVELEP
                     LELNNFIRFDERKYGSTALLFYRRKPSPSNAA"
     gene            complement(30122..31045)
                     /gene="metA"
                     /locus_tag="PK898_04075"
     CDS             complement(30122..31045)
                     /gene="metA"
                     /locus_tag="PK898_04075"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOU19242.1"
                     /translation="MPVNIPDNLPAAETLENENIFVMKGQRATHQDIRPLQILILNLM
                     PTKIETEIQLLRLLSNSPLQINVEFLQMVTHESKHTSQDYLDYFYKTYDQISQNKYDG
                     FIITGAPVENIPFEAVDYWEELCKLMSWSRRNVFSTMHICWGAQAGLYYHYGINKYPL
                     DKKISGIFSHYPVVRNHPLLRGFDEIFYMPHSRYTAVRREDVEAHPELQVLAVSNEAG
                     LAISATRDGRQIFIQGHAEYDVMTLANEYRRDLKAGINPVMPENYFPGNSPKAAPIMY
                     WRSHANLLFTNWLNYYVYQQTPYDIDKISQV"
     gene            complement(31048..32337)
                     /locus_tag="PK898_04080"
     CDS             complement(31048..32337)
                     /locus_tag="PK898_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOU19243.1"
                     /translation="MAANHEWKFETKQLHVGQEQADPASDARAVPIYQTTSYVFRNTD
                     QAAARFSLAESGNIYGRMTNTTQEIFEKRMAALENGTAALAVASGAAAVTYSFLNLAK
                     AGDHIVSAATIYGGTYNLLQHTLPDYGISATFVNADEPGGFEKAIQPNTKAVFIETIG
                     NPNATLIDIEKVAEIAHAHRIPLIVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGT
                     AIGGVIIDSGKFDWAGSGKFPEITEPNPSYHGISFVEAAGPAAFAARARAILLRDTGA
                     TLAPLHAWLFLQGLETLSLRVERHVENALKVVEFLQAHPKVERVNHPSLPDHPHHQLY
                     RKYFPHGGASIFTFNVKGDEKAAKQFIDRLQVFSLLANVADVKSLVIHPASTTHAQLN
                     ERELAEQDIQLNTVRLSIGTEHIDDILYDLNQALEGI"
     gene            complement(32580..33977)
                     /locus_tag="PK898_04085"
     CDS             complement(32580..33977)
                     /locus_tag="PK898_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU19244.1"
                     /translation="MQKNVAGVIVNTKFSPELDPDFIPLPVFMESFLRSATVPFAVAI
                     EREDNQVQVYKTFIHDTPEKEAADRLFAERLVKFMLWSRSGFRLTICGSRKIYEYIKE
                     VYSAGGKRSFDFHFMQDVYDRPFEVLYREYADCPKPFQKSVSIGRNLDGCRIGFDAGG
                     SDRKVSAVIDGVPVFSDETVWNPKVNADPMYHYEGIVDSFKKAAAHMPRVDAIGVSSA
                     GIFINNETKVASLFLKVPPEDFKKKVRTIYIDAAREIGENIPLVVANDGDVTALAGSM
                     SLQKNGVLGIAMGTSEAGGYVDLEGKITGWMNEFAFSPIDLSEYAMVDEWSGDKGCGV
                     KYFSQDGVIKLCPAAGIDLKEEETPAEKLKTVQMLMEENDPRAVKIFDSIGVYLGYAL
                     KLYSMFYEGNEVLLLGRVVSGKGGERMLDMANKVLRTEFPDLNLNVSLPSEKTRRVGQ
                     AVAAASLPKIEKKEK"
     gene            complement(34184..34849)
                     /locus_tag="PK898_04090"
     CDS             complement(34184..34849)
                     /locus_tag="PK898_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOU19245.1"
                     /translation="MTEDEKLIAIYESLLACYGELHWWPAKTPFEVIVGAVLTQNTAW
                     GNVEKALSNFNGDLSPEMIAKTDTTELAEIIRPAGFFNQKAIYVKAVTEWFSRYGYDV
                     SAVRKEPLRKIRTELLATKGVGHETADSILLYAFGFPTFVVDAYTVRLCSRYPIAAGK
                     GYEAIKAYFEEHLPPSAEIYNDFHALIVANAKRHCRKKPICTGCPLELRCEKVGIGST
                     DLS"
     gene            complement(35108..35509)
                     /locus_tag="PK898_04095"
     CDS             complement(35108..35509)
                     /locus_tag="PK898_04095"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HOU19246.1"
                     /translation="MMGELIKNEGAGIIGLFHGEGGSPVVPQPFERDIFLFDTHIAGT
                     SHIEGIEELEPYLNIGDKLDFFREPDNPYDKKAIVIKNADGVKVGYVPRADNVIFSRL
                     MDAGKLLFGRITAKEIQGSWLKINIKVFLHE"
     gene            complement(35511..>37944)
                     /locus_tag="PK898_04100"
     CDS             complement(35511..>37944)
                     /locus_tag="PK898_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU19247.1"
                     /translation="KNRRRQMIGAIIGDIVGSRFEFKNHRSKDFELFGKGCFATDDSI
                     MTLAVAKAIMEASKIKPRSLHGYDDEFYSVLSDLSVKYMQEIGRQYPNCGYGGRFYQW
                     VFSDNPRPYKSYGNGAAMRVSPAGFIGESKQEVEHLAETVTAVTHNHREGIKGAQSTA
                     VAIYLARKGARKSEIRECIVRDYYPLDFKIDDIRPTYRFNETCQETVPQAIECFLESR
                     SFEDAIRTAISLGGDSDTIGAITGAIAEAYYGVPPEIKEKALTYLDDRLRSIYDEWIA
                     FAPQCDERYKVLTKYIGWLSDVKIGNGSTVGFMEDFHYGEFETEWHDSGLVDCQYGET
                     LDKMGVELSYKSITGKDVEDLNAKQVLGLITAAFRNDNFVEGIIMNYLRSGVMGKWLK
                     RLKDIDTEKCSHQITEVELKRINMMGIHTHYVRISDEEASFSTHDEFGDEIGWSTDQK
                     EKITSIRHALDKLHLEYWRMAYPQHFNNHFIKDGEQWILTFQYNDGSVMEINGEETYP
                     ENWDDLLSFFDFDRYRQDKTEREESNEEDPVNSENSQTSVVIVSPDFEKLKSEVEKLR
                     TELSMLVLERDELVLVECKNIEMAYMLTIGGLEYKAYEIQCAIRRLKRKAELIQAKKN
                     RQEKVDPAEIDTLLDVEFSEYQAKLEELVNKMNAAIERSHGKVLSDEEARELKKLYRA
                     IVKALHPDLHPGIEEAKIRLFQNAVTSYENGDLNGLRVIHAMISGPAFSGDEPDAISE
                     LIKEKERITGLIQAVRDRIADAKSRYPYTLKPMIQSREKTEARKAELEESIRQLNEQL
                     AAYTERIQAMLR"
BASE COUNT         9945 a         9080 c         8350 g        10569 t
ORIGIN      
        1 cccgacaacc aaaactctta taaacaaaga cggccggcaa gccgaccgtc ttcttatcaa
       61 aattctttta aacgaattct tacatcagct ttctttcgaa tcgcctttat cggtttcatt
      121 cgaagtttgc ggatgatctt ccgaagtcga atgatccttc atctcggact catgaatcga
      181 atccaacacg gaagctccca cacctctctt ctttttcttc ggcgtagttt ctgcgtatgc
      241 gctactttca cccgtttctt tctgcggttc gcttgcatcg aactccgata cgggttccgc
      301 cccacgcatc tcttcgatac cattttgagc cgagccttct tccgtctcgc cggtttcgac
      361 tttcccgggc aattcaatta cggattccgg tttttgtgaa tcgtttggcc ccttctcgga
      421 tttctcggca accggctctt ctctgctttg tctcattttt tcttcttcgg atgatgactg
      481 aggaattgta ttttccctgg gtcggtaatc tccgctcgga gggttgtttt tgggcaactg
      541 cggattcgaa gtactctttt tcggtcgatc ttggtatcga tgatagtagt aaatcatcac
      601 tccgcccaga ccgaacaaag ccgcgattcc gcccggcatc caaccgacac atgggatgat
      661 tttcttcaat ccatgggcga ccaaagttat gacaaaagtt ccgaaggctg tcgtccagtt
      721 agaagtccaa gtcaccttca tagccttcgt catccattgt ccggcttcat aaccgcaagc
      781 tatccagcca taaaaaatca aaaggattaa agccaagatt aacaaaaacg ccagcggaat
      841 caaaatcaca gtcatgcaga ataaaacaac cacaaaggga gctgcaatca ccgtcaccaa
      901 tccgatccca aaggcgttcc acggtttctc agcgattatt ttgccgatac cttccaaatg
      961 ggaccctaag aggacattgg ccaacaaccc taatccggca ataattaaca ccatggcaaa
     1021 aaacagtccg atacttccga gcacgccaaa aaatgtcaac caaaaacccg ttttgtttct
     1081 atttcctctt gcgaccggtg agaaaacatt cttttcaact cttttatcgg tgttcaataa
     1141 gatttcacca ttttgccgta acagatcacc tttgatgatc gccgtcggtg atttggtaac
     1201 cgtaccgccc atggccgaaa agttcccgtc gataatcgaa ttcggtccga gggatatatc
     1261 tccgccgaac gccgcaatat taccgctcac ctcaccgtcg atgacaatgt caccgccgaa
     1321 aattgccata tctccatcga cgaaagcatc ttcctcgatg gttacatctc ctctgaaaac
     1381 aactaaattc ccgttcagtt tatccccgga ttccaaaaca taatctgaac caaaaacaac
     1441 cttatccttc gcagatacag tcattacggg cagcatcaga atcccgagga ataaaacaat
     1501 tccccacagg aaaaacctat tttttttcat gattgctcta ctccctcatt tgaattttct
     1561 tcattattta tgccgatacg attaatgacg acaaaacaga tcacagcgag aatcccgccc
     1621 agcgaaaaaa tcaataccgg cagcctcagc atggaaagga ttgtacgaaa ctgattggca
     1681 agtcccgcca acaataacaa gatttgactg gcctgtatcg ttgaaggaat caggaattcc
     1741 ggagaaagta atacagtgca tgaaaatata gaaagaaccg taatcaaaat aatcgtcccg
     1801 ataatttgtc gattttgatt tttcatttct ttttcaaagg tacgtacttg caatatctgt
     1861 tcccattttc cggtgatatc agccttcggt ttaaccatcg gggctttcga caacaggttc
     1921 aaacatgatt tccattgttc ttccagtttt tggcattttt cacattcttc cagatgagct
     1981 ttgagatttt tacgctcttc tccgctcaaa tctccatcct tcagaatcaa ttcttcataa
     2041 tattcatgtg aatccatacc atttatccct gattaaattg agtctgtccg tcccaatatt
     2101 gagctaactg ttttctggca cggaatagcc gacttttcac tgcactttca ctcacggaaa
     2161 gactttcggc gatttcccga tccgaaaaat cataccaata tctcaataca atcgccgctc
     2221 gatctgtttc atttaagcta ttaagcatat cttgaatctg ttgttcctcc tcattcctaa
     2281 ttgcttcctg ctccggatga ggaatcggat ccggtatgat ctcttccatc gtctcatcga
     2341 tttctatttc gggaactttc tttttccttt gcagatcaat gcaataatgc gcggctatcg
     2401 aaagtatcca agttgaaaaa gagcgattaa aatcatattt tgaaaaattc gtccatgctt
     2461 tccaaaaagt ttcctgtgtt gcatcctcag cttcggttcg gttatataac atccgatagc
     2521 ataagttata aatcgcattt tgataggcat caacgatatc ggtaaaagcg tcagcatctc
     2581 cttcttttac tttttctaat atgtcggtgg aaatgttgtt caaccttccg ctccaataaa
     2641 ttctatctgt ctatacggaa aaaacgagaa agagttgcag cgcatgccaa caacgctaaa
     2701 tgatctcaat tcccggtttt tccaccgctt cgccgatcac ccatgcactt tgccctgatt
     2761 ttttggcttt ctccaaaaat tcgtcaaggc gatcagaacc aatacccaaa agcaagccgc
     2821 cgctggtttg cggatcaaat aacatcattt gttgttgctc ggaaagagcg attttaaaag
     2881 tcactttatc tccgtaataa ttgcgattat ccgtagcccc gcccgggaat aaccacatat
     2941 tcatatactt ttcataatga tcaattttgg ggatagcatc ccaatcatat cttaaaccga
     3001 ttttacccga tcgaatcatc tcccaactat gccccattaa actgaatccg gttacatccg
     3061 tacccgaatg gcagccgacc gacaccgcca attcggatgc cgttttattc agctttttca
     3121 tccaatccac cgttttttga aaatcctccg aatcagctga gccgttctta ttcgcagttg
     3181 tcatcaaccc gaacccgatc ggttttgtta ataccatttt atcgccgact ttcaattttc
     3241 ctttatacag aatccgatcc ggatgaaccg ttccaaccac cgccagtcca tatttgggtt
     3301 cctggtcgcg gacggtatga ccgccgacga tcggacaacc ggcttctttc gctttttcag
     3361 ccccgccgcg aaaaatatcc gcgatgactt ccaccggcag gttggtcggc atatcggtaa
     3421 tatttaatgc catcaacggc ttgccaccca tcgcataaat atccgatata ctgttggcgg
     3481 ctgcaatagc cccgtaatca tagggattat cgacaatcgg cgggaaaaaa tccgtagtga
     3541 ataccaatgc ctgttcatca ttcaatttgt aaacagccgc atcatcagct tcttccaaac
     3601 cgatcaacag ctggggaaat tcattctggt taaataagcc gccgaacggg ttgatgacat
     3661 ccatcaagac ttccgctcgc agttttgccg cacaacctgc gcaaaaggca agttcggtta
     3721 atttgatcgc ttttttctct tcgctcattt ttactccaat cggtaaaaca aatcccttat
     3781 ttattaatta attatttttt gaatttctat tataatatgg aatgttcggt aataccggac
     3841 gcgagaggga ctgtgaatgg ggaatcattt gattcgacca gtcggaaaag tctgcgtcat
     3901 ataatgaatt catttgaatc tgtcaccgag cgatccattc tatctatcgt aggagccggc
     3961 ggaaaaacat ctttattgtt ttatctggcc gaagttttgc cttggaattg tcttgtcaca
     4021 acgacgacaa aagtcggtcg ggatcaaatt tcctccgctg accgaatcgt ttcattttcg
     4081 gaattccttg ctgcacccga tcacttttcc ggggataaaa ttgtctgggt cagtccggcg
     4141 ccggaacagg attatccgaa agtcaaaggg tttgatccgg ctcaattcag tgaattcgcc
     4201 gaagcggcac gaaagataag aatgccgatc ctcgttgaag cggacggcgc tcatcggtta
     4261 catctgaaag ctccggcgga aaacgaaccg gtggttcccg cggaaagcga tcatatcctt
     4321 gatgtcgtgg gactcgatgt gatcggattg gcgatcggcg agcggacagt ccatcgtttg
     4381 tctcaatttt taaggataac cggatgccga gaaggggaca ttatcgatga atccgttatc
     4441 tgccgtgcca ttcttcaccc gcagggggga tttaaaaacg caccgcaagg atgttgcaag
     4501 atcgcggtat tgaatcaggc tgacactgcg gaaagaataa agcacgctga gaatatttca
     4561 aattatttac tgaaacacgg cgtagatgaa gtctggatca caagtctcga tcccgaaacg
     4621 gataaaaata aacggggaat tataaaggaa ctgaaaaaat gaatcaaaaa aactgccatc
     4681 cgagagaaat cactgccttt cgcggagcgg gtgatttggc aagcggaatt gctttgcgtt
     4741 tatggcgttc cggttaccgc gttattttga gtgaactgcc ttgtccgtta tgcattcgac
     4801 gcagtgtggc gtttgccaat gcggtctatg aaagccatat ggcggtcgag ggcatcgaaa
     4861 gcatcctgat ccacgatatc gccgaagcgg agacgatttg ggcggaagat aaaatcccct
     4921 tattgattga tccggatatt tctcaaatca aagaattaaa tccttcggtc attatcgatg
     4981 cgcgaattat gaaatcttat cgggacgatt ctcatttgag tgatgctccg ttagtcatcg
     5041 gattaggtcc gggtttcgtt gccggagaaa atgcacatgc ggtaatcgaa acaaatcgcg
     5101 ggcatgattt aggtcgggtt ttctggaaag gcagcgccga gccggatacc ggaactcccg
     5161 gaatgatcgg cggtgaaggt ccgaaacggg ttcaaaaagc tcccgccagc ggcatcttta
     5221 ctccgaaagt cgaaatcggc gatttcgtca aagaagggga tttattggca ttggtcggag
     5281 ataccgaaat tcgcagcccg ctgaccggca tggttcgcgg tgtcatctat ccgggaattt
     5341 tcgtgaataa aggcttgaaa ataatggata tcgatccgcg cggagagcgc cggtattgtt
     5401 tcacagcttc agacaaagcc aacgctctgg gcggatccgt tttggaggct attttaactt
     5461 ggaacgttcg agaatcagct cggcaataat gctgatcgca atttccgtgg gggtttcacc
     5521 gttgatcggc aatccgatcg gagaatgaac ccgatcaatc tctttttgtg agacaccttc
     5581 gttcaataag cgttcataaa tcaaatttcg tttatgaata ctgccgatca ttccgatata
     5641 aaatgcatcg gtcttcaaca tttccttaag cacatcataa tcgcctaaat gaccgcgagt
     5701 caaaatcacc acaaaacttt gatgatcaat tggaagatcc ggtaagcttt tataatcggg
     5761 aacgaccatt attttcgaat tcggaaaccg ttccttattg gcgaattcgg ctcgatcgtc
     5821 cagtacaatt gtctcaaaag aaacctcatc ggcaagccgt gcggtttctt tggcgacatg
     5881 accgccgcca aaaatgtaca gcttggttcg ttctttaatt ttttccacca gaatttccga
     5941 attcaggaga agatcggaat gaatggcaat ctgatccgga gttcctctca tatccgatag
     6001 gaattccccg ttgataatca aatttccggt tttttctccc tgcctatcta caaaacaaaa
     6061 cttatttgta tcgacgccgt tttctcgaaa ttgcgttatc agccatcctt cttggttccc
     6121 gacagctgca tccagtgcct tttcaaatac aagggttgtt tccggatcat cccaggggag
     6181 gaaaaccaac agaacatctc ccgatccgcc gcagatcata tcggatttcg ccgcatcttc
     6241 accgttcaaa atgaaatgaa aaagattgct cttcttcgac tgaaaaacct ccttggcagc
     6301 cagaatcgca ttcgcttcga gttttcctcc gccgattgta ccgataatcg aaagatcggg
     6361 tttaatcaac atttttgtcc ctgccgaccg cggagaagag ccgacattat ctatgattgt
     6421 cgccatcacc aaatcttccc gtccggcatg ggcatcgaca atcgcctgta atatttctcg
     6481 cattatttat cctttaaatt gaaatatgag aaccccggtc tgtttaatct tcttcgttct
     6541 tccgaatgga cagacattca ttcaaccaag tgacgtttga ccgcaaactc tccaatcgaa
     6601 attgatagac catcctgaga taaacggcat catccggttg gtttttcaaa tgagtgtttt
     6661 gtaactcgat ccattgatta caaagcataa tttgttgttt aatcactgat tcaaaaattt
     6721 gataaggttc atctttcaaa aaatagagct tgaccaaaaa atccgaacga agctcacgcg
     6781 gtttttcaac cggtcgaact ttccaggctt gaaaagcatc ttcgccttct tgcgttaagg
     6841 tataaatttt tcggaaagga aacgcggatc cctgagtaat ttccgattgg atcagattat
     6901 ttttttccaa tttatccaaa atcgcatata actggctctg attaaatcgc caaacgacac
     6961 tgaattcttc tttttctttg agcattttat ccaaatcgta agcatggatc ggttgcttgc
     7021 gaatgatacc caacaataaa aactcaacgg aaagcggatc ttgtttacgg gacatggctg
     7081 atactcctct ttcttaccaa ttttatcaaa aatttgttca attaataagg ttttttaata
     7141 tcgatccatg caaaatcctg ttttatcaac aaaatcctca aatttgaaaa tattatggta
     7201 aaatcatttt ctgctgggtc gaagggacaa cagcattcga tcgaaatccc gatttcgtaa
     7261 atagtttagg gttcgtatca caatcgggag tcctgaagaa caaacggaag gaaaggaaga
     7321 taaaaatggc aaaatgtgaa atatgtggga aaacgactgt attcggacac aacagaagtt
     7381 tttctcaacg ggcgacaaac cgtgcatttt atcccaattt gcaacgtgtt accgtttatg
     7441 aaaacggaca gcgtgtccag aagaccctgt gcacgaaatg catccgcacc atggcaaaat
     7501 tacccaaaga atgattcgat acggaaactg atcgctccaa gcagatccga tgacttggag
     7561 cgatttttta tttaaaccag tctcaacaaa gattttttaa ttcgcgaata cctgcggcat
     7621 aaccgttgct gttccgaaag atggacgttc ccgcgacaaa agtatcggca cccgcggcaa
     7681 agcattgccc ggcagtttca tggttgattc ctccgtccac ttgaattcgg acattcaatt
     7741 gtttttcatc aatccattta cgaatctttc gcattttttc caacatttcc ggtatgaatt
     7801 cttgtccgcc gaaaccggga ttaacagtca tcaccaacac catgtcaaga aaaggcaaca
     7861 gaggttcaac catttccgca ggtgttccgg gatttagcgc aattgccggg ctggcgccca
     7921 ttttacgaat atttaagaga gtggaatgaa tattcggatt attttcgcaa tggacggtca
     7981 aaatatccgc cccggcttca ataaatcgtt cgatatgttt ttcaggctgg ataatcatca
     8041 gatggacatc caacggaagc gttgtgatct ttcgaattcc ttcgataaac gggaatccca
     8101 gcgtcagatt ggggacaaaa acgccatcca tgacatcgac gtgaatccaa tcggtccctg
     8161 atgcggctac cgcctgaacc tcttcaccca ttctcataaa atcggaggct aatatggaag
     8221 ccgaaacaat aatctcattt ctctgcatgg ttattttcaa tccttattga tgataggctt
     8281 ttgtaagtgc tatgaatagt tcgccggcat cccgatatcg ctcctgcgga tttttacgaa
     8341 tgcattcccg gatgattcga tcgatatcat cggggagatc ggcaatttca tgcggcggct
     8401 gcggcatccg gttgcaatgg aagtcgagca tttcttcggg atcctcgctg taaaaaggca
     8461 gctgccccgt atataactga tagagaataa ttcctatgct gtaaatatcg gttgctttaa
     8521 cgggttgctc tccgcgacac tgttcgggcg acatatataa cggggaagcc caaacaacct
     8581 ccggcttttg ccaaccggaa tcctccgtcg attcaagggc aaaagcctca ccgaaatcga
     8641 tgattttcaa atttccgccg gaggacaaca gaagattgag gggtttcaaa tcgcagtgaa
     8701 caatatccat ggcatggata gaccgaacgc cggaaacaag ctgaagcgca atggattgtt
     8761 tggcggaatc cgtaaattca ggcttccgat gatatgtttg ccatagattt tcaccctcca
     8821 catattccat aaccatacaa ggagaatttt cccggtcgat taaatcatat acctgaacga
     8881 catgcggatc ccttatccga gattgaagat aagcttcatg aattattttt tgctgttggt
     8941 atcgatcctc tcgatgttca tcatttaaaa ctttcagcgc gacttttctt tcacgttttt
     9001 gatcagtcgc ttcatatatg accgtggtaa ccccgttagc aagcatacga atgatatgaa
     9061 attgactgat ctcatcggaa tctgttttca ttgctatata attatagctt gaacaatctt
     9121 catatgccgg agagatatgg cagacaatca taaaaacaga atattcaatt tatcaaccgg
     9181 tctgatatca ctctgtgtcg cagcttttat ctttttggga atcatcctat taatcgttaa
     9241 catcggcccg agccccgccg aagagaaaga agatgcatta gctcaattaa cggttattcc
     9301 gatgcctacc atcactccga cttccgcaat tccttcggaa aagcccgatc tcagcgtcaa
     9361 atatatatca ccggaaggtt tttccgtcgg tgcatatgta aagattgaga acactcaagg
     9421 agccggatta aaaatccgac ctgacccggg aaccggcgga gaagtctctt tcatagcatc
     9481 ggaaggggat attttcatta ttatcgacgg ccccgatgaa cggaacggct atacttggtg
     9541 gaagctgcaa ggatttgaag atccgtccct ggtcggttgg ggggcatcaa aatttttttc
     9601 attggttgct ccggccgcgg atcaaattca atcatcggat attcaataat gagtcataat
     9661 caatcaattc atgcattgga atcggcaaat gctcactcta tcggcaaaat aaggcgatat
     9721 aacgaagatg ccttggtttc tttccagctt gaaaccatgc gtaaagatca aaccgattca
     9781 ctcggcctgt atgtggttgc ggacggaatg ggtgggcata tcaacggtga tgaagcatcg
     9841 gcgtctgccg tatccgcatt tacggaacaa ctcggaaacg atatcatccc gttgttcctg
     9901 aaagatgaat cagattcctc gattatcccg gaattgttaa aaaaggctgt tcttttcgct
     9961 aatcaatcga ttctggatgc gcatcccggc agcggaacga cttttacggc ggcattgatt
    10021 tggaatgaaa aaatttatat tgcccatgtc ggggattcaa ggttattttt gattcatcaa
    10081 aattatgaaa tggagcaatt gacccacgat cattcgttgg tccaaatgat ggttgatttg
    10141 ggagaaatca gcgaagaaga agccctgaat catccgcggc gcagcgtctt actccggagt
    10201 ctgggattcg acaaggatat agaagttgat ctcgccgaaa acaagctgga agccggagaa
    10261 tcgttgctct tgtgttgcga cggtctctgg tctgtcataa agaatgatca aatgatcaaa
    10321 atcattcgcg aacataacga catacaggaa gcggccgatg cactggtgga tgcggcgaat
    10381 gaagcggggg gaccggataa catcagttgt attctcgtcc gccgaaaata agataaattc
    10441 ataatacaat taagtaacgg tttcgggctg ttccgaccag atattttacg aaagagagag
    10501 gcaaaaatgg ctgaagggaa taaacgctat ctgattttgg ttgtcgatga tgatgagaga
    10561 attgtcagaa tgattcgtct gaaccttgaa catgacggat actctgtaat cgaagcaaac
    10621 tccggaatgg aagcgatcaa tcaggtgcgc gagaaattgc cgcatctcgt gattctggat
    10681 gtaatgatgc ccgggatgga cggttttgag accctttctt tgattcggga gaccagtcag
    10741 gttccggtta ttatgttaac ggcaaaagga gaggaagaag atcggatccg cggattggaa
    10801 ttgggggcgg atgattatat taccaaacct ttcagcccga gagaactcat gagccgcatc
    10861 aaagccgttt tgcgacgtac cgaagggaca agcggtatgg ataccggaga agtcattgaa
    10921 gtggatgaac acttgaagct cgatttcggg aaacgggaag tatggctgga tggccaattg
    10981 gtaaaactaa ggcctaccga gtatcgtctt ctttatcatt tggtgcaaaa tgccggctgg
    11041 gtgatgacgt atgaccagat tttggcgaaa gtttggggat atgaatatcg ggatgaaacc
    11101 cattatgtcc gattatatat taattacctg cgacagaaat tggagaaaga tccctccaac
    11161 ccgaaatata tcctgaccga acggggaatc ggataccgat tcgtcgatta taaaaaagga
    11221 aaattgaaag aaggcgaagg ggagacgaaa gaaaatccga acaatccaga ggagagtcac
    11281 ccctccgata attgattaat ctttttttta tagaattctg ataaagcgca tgcaattttc
    11341 ttatacatgc gctttattct ttaaggcgaa gatagaaaaa agacagcgtt ctgaaaagaa
    11401 cgggaaaaac gaaaatggaa ataaaaggag ggagctatgt acggcaaatt aagagttcaa
    11461 ccagtaagag atttatttac gatgcaagat gcgatggatc gcttgttcaa tgagtttttc
    11521 ggagacagcg ataacctgca aaccgaagtt cttgcaccaa gggtagattt gaaacaaaag
    11581 gaaaatgaga ttgatgtaaa gctcagtatc cccggcgttg acccggacaa gattcagatt
    11641 tcagtcgctg ataatgtttt gacagtcaaa gccgagatgg aagaagaaaa agaagagaaa
    11701 gacgagaaag ctgtttacga aatcagagag catcggtgga cttcctatta tcgggagatt
    11761 gctcttccgt gcgaagtgga agccgataaa gcggaagccg aatatgaaaa tggcgttctg
    11821 aagattaaac tgccgaaggc tgagattgtc aaaccgaagc tgatcagtat ttcagccggt
    11881 aaaaaaggcg aaaagagtac tgaaaagatt gaaagcaaaa caggcaaata aaggataaac
    11941 ttgtagtatc gagaagcccc atctgaaatc agacggggct tctccctttt aaattcccgc
    12001 atggtcagga acgttaatta tcgttataat ccattttatg caactgacgg atacttcgct
    12061 ggttctgatt gcaatcatgc caaaattacg cgatatggaa attgcccgtg ttttgggttg
    12121 gtatcgaatt ccgatgaaaa ctgcgccaaa aatattgttg accgattaca ttgcttttta
    12181 tcagacaaag gattttggcg aagagcataa aagccgtatc gaaaaatatg cggaagttcg
    12241 cggcgtagaa ctgacgacac gccaagaact gttccgggat gaacccgatc atccgcgagc
    12301 gaaggaagaa tattataaga ttcaaatcgg ccccttgcag gcgttggaaa atcccattct
    12361 ggccgatcga tggaagcgat ttttattttt ttacacttcc ggcgaaaaga tgtttacggc
    12421 aagcacactt cacgatttgg caattaccgg ttcggaacgg gaaatcttat ggaaatctct
    12481 tcgggaacgg cgggaaaacg atccggaatt ctttagatcg actgaaaaga aagaagaaat
    12541 tcccgaacag ttgatgtttc tcttggggaa tttaaccctt tctaacttag attttaatga
    12601 gtaatccgat tatagaattt accgattttg ttttgtggaa atatgggagg ataaatggaa
    12661 aaaattatta agaacgggaa gttggtgacc gattcggaga ttttcgacgc cgatattctg
    12721 attcgggatg aaaaaatcgt cgctgtcgga aaagagttca atgaatcgaa tgccgaaatt
    12781 attgacgcaa gcgggaaact ggttttgccg ggcggtgttg atgcccatgt gcatttggat
    12841 ttacccatgc cgggaacggt ttcttcggat gatcattata ccggtacaaa agccgctgct
    12901 ttcggcggaa caacaaccgt tatcgacttc gccaaccatg atcttccttc tctgatagac
    12961 agttttaatg tttggcagga aaaagcattg ccgaatgttg cgatcgatta tgggattcat
    13021 cagaatttca cccgatttaa tgagaacacg ctcaaagaaa ttcccctcct tccgaagatg
    13081 ggcgtgacat cgatcaaaat gttcacggca tataacgggc gaatgcggtt acaggacgga
    13141 gagatatttc aagccatgcg tgtcgcgaaa gacgaaggca tcatctcact ggtgcatgcc
    13201 gaaaacggcg atctgatcga ccttttggtt gccgaagcgc ttgccgcggg acatacagct
    13261 ccggtttggc aagcccgcac ccgtccggca tggggagccg ttgaatccgg ctttcgagtg
    13321 gcggcattat cgttcagtgc cggaaaggct ccgttgtata ttgtccatat gaacgtaaaa
    13381 gaagaagcgg atatgttgct ttacgcacgg gaaaacgggc tcccgatttt cggagaaact
    13441 tgtccccaat atctgttatt caccgaaaaa aatctggaac agcctgacgg cgccaaatgg
    13501 gtttgttcgc cgccgatgcg atctgaaaaa gacaacgagg gactttggga ggcattggca
    13561 gacggaataa tcgatgtggt ttccacagat cattgtcctt tcttatttga cgggacaaaa
    13621 ccgatactct atgaaggaaa accatatcaa caacccggaa aagagctcgg caaagacgac
    13681 ttttcaaaaa ttccctgcgg cttgcccggc gtcggcgatc gcatgccggt actttggaca
    13741 aaaggtgtta acgaagggag gatcagcccg actcgttttg ttcaattaac ctgtacgaat
    13801 ccggcaaaaa ttttcggaat ttacccgcaa aaaggggcac tcttgcccgg ttcggatgcg
    13861 gatctggcga tttgggatcc gaataaaatc gtttcttacg gagtcaagat cgcaaaacat
    13921 cgaaccgatt acaatcttta tgaaggaacg gaactgaagg ggttcccgga aaaagtcttt
    13981 ttaagaggga gtttaattgt cgatggagac aattggctcg gaaaacgcgg cggaggacat
    14041 tttctgaaac ggtcccgttt tttgggatga ttcgaatatg cgttttctga ccgttccgat
    14101 cggcattctt tcctttgcgg caattctgat ttttctgatc acgaggctgc gaaaaaagat
    14161 cggcgtcgca tggttcatcg gtgcattggc ttctttttcg gtctggtgtt ggatttttta
    14221 tttaaaacga acaccggaaa gcgttttttc gattcggtgg caaaatctag aactcggttt
    14281 tcttctggat aatatttcat ggtcttacat ggcggcatca gccacaatgc ttttttttat
    14341 gatggcttcc gctccttccc gcttgaatcc ggacagttcg tcaaatgatt ggggattttt
    14401 tctcctgatt accgcaatcg ttatggctac agtcgcttcc gattccggtt ggtcgatggt
    14461 tttcggttgg ttcctttttg atttgctgac ggtcggtatc caatttttct ctcatcagaa
    14521 aatctaccct ttcgaatccc tggaaaagat attatttatc cgtatcttca gcattttttt
    14581 agccgcagtg gcgatagcga tcgggaattc gattgaattg aatcccgcgc aatccttttt
    14641 ggaagggcaa cgcgaaacga ttctcctcat cgcctgcgcg atgcgagccg gcttattccc
    14701 ttttttcgaa ccgtttcaaa atgaaaacga aacgagaaag ggactgaaca catttgttga
    14761 actgtcatcc gtcgctttgg tgttacctgc ctttattcgg atcgattctt ccgccatggg
    14821 taatgacgcc tacggctttt ttatgttttt ttcaacctta tccgttattt gcggcgggct
    14881 cggaatgatt ttttcaaaca aaaacgcatc cgaacgccgt tattttgttt tgacccttac
    14941 cggtttatct tttatttccg cattgcgatt ccaaccgacc gcatccgtcg tatggggaat
    15001 atctttatcg gtccttaccg cgcctttgtt cctctatgat atcaggagcc ggttgttgaa
    15061 tgtcgttcag gtgatttccg gtttgcttct ctcaggaatt ccgcggacgc cggcagcctc
    15121 cggttggcgg ggattggtgc tcgttccacc gtcttttttc gactttttcc tgatgatctc
    15181 tttcgcctgc ctgcttacga agttcttttc ggatttaacg gttcaaaatc ccgctcaccc
    15241 cgctctgcaa gatcgccgga tgaatgccat ttatcctttc ggctttatgg tttccgtgat
    15301 cgcctacgga gtgatcgctg ccttgagttg gaatttttat tcggataacg gaatgatccc
    15361 ggcatcgatt attacgctga tcatcgcttt catcatcgga tttcgatccg tctggctaag
    15421 atttttcact cagatgaata ccttcttcag ctggtttcgg gaaatttttt ttacgatttg
    15481 gaaaattttc ttatggatca ttcaatttcg gtggttggaa gctcccatca gatggatcat
    15541 aaccggttta tccgccgtta tcgatttcat ttcgggaatt tttgaagctc cgggcggcat
    15601 tctttggaag ttcctgattc tgattatcgt attatttctg gttatcggta tttaaggaac
    15661 agcatgcgtg gtcttttctt tacctcaatt atcggcggcg cgttgatcat cctcggtgct
    15721 ctcatcattt ttatccgatt gaatcagccg gacagccgaa tcagtctgat cgcttatatt
    15781 ctcgagtctg tcggaatcgt tctgctgttg gttcgaattt tttctcctca gatcctgatt
    15841 tccgtcttcc tcactggcac gatgggagcc gccatactgg gaaccgatca ggttgccctt
    15901 catatccctc tgaaaccgga taaagtcaat ccgcagggaa ttttcaggct tctcctcggt
    15961 ttcattcttg ccgttttgat cctctcgatc gaacccgaaa tagcggtttg gataccggtg
    16021 ccggactcga tcttattctg ttccttgttt ctgatattaa tggggctaac caccttcagc
    16081 ctcagctccc gcctgatgta ccgtttcttg ggaattctga atattttcag cggctttcaa
    16141 ctggtttata tgcttttgga agaatccgtt ctcgtttttg ggtttatgat tgccatcgac
    16201 ttgctgatcg ccctattggg ggcttatttt atgagcggtt cacaaaatga cgaatacccg
    16261 acctgatttt gacaggaaag cttaaaaatg aatgcacctc tcctttggtt tattatcccc
    16321 tttttcttcg gagcttttct gctgctattt tcggaaagca aaagattatc gattattctt
    16381 gcctcaattg tctgtctttt tctgattgcg atttccgcaa cggttccgat caacagcatg
    16441 gtgattatcg gaaaaaattc gatcatcttt accgattcaa tggaaatact cggaagaaca
    16501 ttatcgcttg agagagcggc gcagcccctt ctcgctttct tttattcata tgcattcatc
    16561 tggattcttt tttccgctgc cgttcgggtt catcgttttt ttgtaccgct ggttttgatg
    16621 gaaaccgctt tggtaatcgg tgtgacggcc gtttcccctt tcatctatgg tgcttttttt
    16681 gtccaattgg cggtcttgat cgctttaccg attttttgga acgagaaaat gaaatccggc
    16741 gagggaatta ttcgcttttt gctctatcaa accttgggat tgatttttct ggcgatcgga
    16801 ggctggctgg cttccgcggt ggaactcaac ccttccgatc aatacatctt gaaaagaaca
    16861 atggtcgtcc ttctggtcgg ttttattttt tggttggcga tttttccgtt tcacagttgg
    16921 atcgcaatac tcatggacga aagctgccct tacctctcgg gttttgtgat taatatttta
    16981 caattttcca gttttctgat cctgctgtat tttcttaaat cctatttgtg gctgcgaacc
    17041 tatgatccgc tttttattgg attgagaatg atcggcgcga tcatgttaat catcggcagt
    17101 ctctgggctg tttttcaaaa atcggttcaa cggctccagg cttttttaat tgtggctgaa
    17161 aacgggattg ccttgttttt attgggactc catacaaccc aatccttttc ggtttttatt
    17221 tcgttactgt ttattcggat gatcgcatct ttcttctggg cactcgccgt caaatccttg
    17281 ccggaaaatt ttgacttcgg attgaattcc ctgcaggggc tttatcgttc cgatccgttc
    17341 atcagtttga cactcatcgt cagttatttt tcaaccgtcg ggttaccgtt tctgcccggt
    17401 tttccttatc aagcgacatt ccttcagctt gctttttcag aatcgtcatc catgggaacg
    17461 gttctttcga tcggatattg gctgctgatt gcggcgggat ttcggttggc agtgacgttg
    17521 ctccacccgg ctgagaaagc tgccggaaaa gcggaaaccg caaatcagcg agctttattg
    17581 atcatcggaa tcgccgcact ttttttgatt acttttttcc ctgaaccctt caatcggttt
    17641 attgctcaga ttcaatctca atttacactg atcagcggcg ggtaatgatg tcctttccgg
    17701 aaatagtata ataaaaccag tcaggagcga atgaattatg gcaaaaactt ggaacggcgg
    17761 accactcaat gaaggatttg ttgatctcga ccagcttgtc aaacggcttg aatggcttga
    17821 caatgagcgg agaaacgaca aaaaccgaat tctcgccatt gaagagcgcc tgacagacgt
    17881 tgtcagtgaa aacgttttgc ttaaaaatca gatcaaagac ctggaagaag aaattcagag
    17941 atatgcgaat attcaggcga aaattgagca gaatgaatcg ttgattgcgc aattgcggat
    18001 tgatgctttc agagcgatcg aagaaaaaga taaagctcac aacgaaagag aaaaagaaat
    18061 cgatgccgct cgtcgtaacg gaatcgattc cattaaccgc actctggccg acctgcgtaa
    18121 atcgattgag cctcttcaag atatccgaaa aacattgctt tcgcgaaacg aggattccat
    18181 tcggttgggc aggatgatcg aagaattgca agccaagatc gatgcggttg ccatggataa
    18241 agatgaatat gccagattcc aaaaatccgt ggaggaaaac agaaggcaag acgccaaacg
    18301 ggtgactgac attcaagccg aggtcaccgc ttaccgaaaa cgaatcgagg atatgagaag
    18361 caaattcgat ttggtaagtg aaggtttgcg gaaagtagaa cagcggatga gcgaattatt
    18421 ggcagcggaa aatgaacgaa agcaatccgt cactgatttt atggaaaaac aatcgttagc
    18481 caacctggaa tatgaaaaga cctggcagca atggaaaacc gcctttgatg atattgttgc
    18541 caaagccgcc ggattggata ctcaaattga gacattggag aatttacagc gttccgtgaa
    18601 acgctcgcag gatacattgg atgaggtcac tcaacgtttt gatcggcgca ccaacgagct
    18661 ggttgaaatg caacgcctca tggaagaaaa actgcgtcag gagtgggcga atttcaaggc
    18721 ggataatcaa aagcgctgga ccaattattc gttagggcaa gaagaaatac aggcggaata
    18781 tcgacggaat atcgaagctc ttcaggccag aatgcaggct atcgaagatg ccagcgcctc
    18841 agctttggac gacgttaatt taagagatga actttatatt tcgcaattac aagacttgtt
    18901 tacaatggta cgtggctggc ttgagaaaat gggtaaggca agctgatcac aactttaata
    18961 ttttatgatt aaggagaatc cttcatgagt caaatcacag tgataggaac gggctatgtc
    19021 ggcttggtaa cgggagcatg cttttccgat ttgggaaaca atgtcatttg tctggatatt
    19081 gatgagaagc gaatttcgga tctcaatcat ggcattatgc ccatcttcga accgggattg
    19141 gaagaaatcg tcgaacgttc acataaagcc ggtcgattgc aatttacggt aaattatccg
    19201 gaagccctga gagatgccga attcgttttc attgccgtcg gaaccccttc cggatccgac
    19261 ggagaagcgg atatgagcta tgtcgagcag gcggtcatac aaattgccga caacatggat
    19321 catcccatca ttattgtgga taaatcaacc gttcccgtcg gcacgggaga ttgggtagcg
    19381 gacattattc aaaaataccg tgacggtcat atgccgaaat ttcaagtcgt atccaatccg
    19441 gagttcctcc gtgaagggtc tgccgttgcc gattttatgc atccggaacg gattgttttg
    19501 ggctccaatg ataccgaagc ctgtgatcgg gtcgccgaat tgtatgcacc gctgcgggct
    19561 ccgatgctgg ttacggatat ccggacggcc gaaatgatta aatatgcctc gaatgccttt
    19621 ttggcaacca aaatctcttt tatcaacgag attgcgaata tttgcgagag tgtcggtgcc
    19681 aatgtgatcg acgtagccag aggcatgggg ttggataacc gcatcgggtc gaaattctta
    19741 aacgcaggaa tcggttgggg aggaagttgc ttcccgaagg atgtaaaggc gcttgcccat
    19801 atcgccgtgg aatacgggac acagccccag ctgttgcaag ctgtgatcga tatcaatcaa
    19861 acgcagcgcc ggcgggtcgt tgaaaaactg aatcggatgc taaacggatt ggaaggaaag
    19921 gaaatcggta ttttgggctt ggcatttaag ccggataccg atgatatgcg cgaaagcccc
    19981 gcggcggata tcatccgata tctgacgaat gaaaaagcaa tcatccgtgc ctatgacccc
    20041 gaagcaatgg agcaggcaaa gaagattctg ccgaaagaag tcatcctctg cgataaccct
    20101 tatcaagtgg ttgaaaacgc ggatgccctc cttctggcga ccgaatggaa tgaattcaaa
    20161 caattggatt ttaaaaaaat acatgacctg atgcgcgaaa agaacatttt ggacggccgg
    20221 aatctttggg atcccgaacg tctttcggaa ctgggcttta cttatgtcgg cgtcggtttg
    20281 ggaaaataca tttgacaccg atttaccatc gatcataaaa attcgaactt aacgaaaaca
    20341 gaagcgcatt tctttaaggg atgcgcttct gttttttata attcgttatt cttattgttt
    20401 atcgttgttt attcttgttc cgtttttaat aggaaataat tcttctttcc tcttcggagg
    20461 accagatatt tcccttcgat aaactgatct ttccgaatgg aagtgttcac atccgtcacc
    20521 cgatgattat taatataaac accgccctcg gcgattgccc gtttgacctc tcctttggat
    20581 ttcatgaatc cgcaatcgct cagcagattg acaatgggca gcccttctcc gttcattgaa
    20641 tccgccgcaa tcactccgga cgggacatca gcgaaaatct ctttgatatc cgcagccgag
    20701 agaccgttga tttcaccgcc gaacagcgct tcactggctt gcatcgcttt atctaaagcg
    20761 gtctcgccgt gcatcatttt tgtcatttca accgccaatg ctttttgggc cgcccgacct
    20821 tccggatttt ctcgggttgc aaattccaat tcgtcaattt tttgtcgatc aaaaaaagta
    20881 aaatacttca ggaaagggat tacgtcggca tcatcggtat tcagccaaaa ttgataaaac
    20941 ttgtaagggg aagtcctttc cggatcaagc cacaccgcgc caccggcagt ctttccgaac
    21001 tttgtgccat cctttttctt taccaacgga taggtcaatc cgtaagcatt tttaccggtt
    21061 tttcggcgga ttaattcaca accggccgta atattgcccc attgatcact gccgccggtt
    21121 tgcaatacac aattttcggt cacgaacaag tgataaaagt cataagcttg taagaggctg
    21181 taactgaatt cggtaaaaga taagccgctc tcccgagaca agcgcgattt taccgagtct
    21241 tttgccatca tgctgtttac ggtgaaatat ttcccggtat ctcgcagaaa atcaatgagt
    21301 ttcagatcaa gcaaccaatc gccgttattc ataatgcgag ccggattgct ttttatttca
    21361 aagtctaaaa atttcgccag ctgccgcttg attttctcga cattgtaatg agcttcttcc
    21421 gccgaaagca ggttgcgttc gtccgatctg ccgctcggat caccgatcat tcccgttccc
    21481 tcgccggcaa gcgcgatcac atgatgaccg taacgttgga agcgggctaa tgccagcatc
    21541 ggcaccaaat gcccgacatg caaactgtcc gcggtaggat caaagccgtt ataaagcgtc
    21601 accttttcct tttccagtaa ctcttccaaa ccgtccatcg aatcataaat catctcacgc
    21661 cattttaatt cgccgtaaat cgaatgttgc tcactcattg tcgctataaa ccccttcttg
    21721 atttgtctgt ttttattttt aaatagtaaa gcgataaata ttcgtttcgc gcggttcaaa
    21781 tccgcaacgg cggtagaggc ggttcgcagc ttcccgtgac ggtcgggagg ttaaatcgac
    21841 cgtttttgca ccttccgctc tggcacgatc gacagcggct ctgaccaaca tttcaccgac
    21901 accccgaccg cgcatttgct catctacgac tacatcctca atccacgatc ggacgccggt
    21961 aggcaatcgg aaaacagcca acgtcagact acccacaatt ttgttattct gatccggatc
    22021 caccgcagcc agcaaaatgg atgcccggga ttgaatcaga tcttccaact cgtcccaggt
    22081 cggaattttt gccgaggagg aaagctgagg aaccaaaacc gtaaatgcgt ctaaaacggc
    22141 ttgagacatt tcttttattt caacgatttc catttttatg ctcttttcta aaaatgtcaa
    22201 atttctgctg gttattatac tatgtgaaaa aatggtttaa atcgttcttt agaagccttt
    22261 tttttcgcaa aatattccga aatcggtaaa aataatgata aaacagttca tagacaatcc
    22321 gaaaagttaa tgtatcatta ccgaaagatt atgtcagacc ttacggagcg aatttcgcaa
    22381 acaatgataa ccggttcaga aagagtgcat cccgatgcgg caaaaatcat ataaaaagga
    22441 tcgacgaaac ggcagagggt tttccgtttg tattttaatt ctggcattga tttgtctgat
    22501 ccccatagcg ggcggatatg aattcacgct gtccgccggt gaatttcttg cgccggacgg
    22561 tacggttgaa tcattcaccc ccgaggttta ttcatggctc cgggcagtgt tttcggttct
    22621 ctttcttgtc ttaatgggac ttttcatcac atccgtctgt attcccgaac gatttttcga
    22681 attttggcgg gaacaatggc gatctttcaa aggattcccc tcggcattga aaagtgacac
    22741 tggcacgttt ttcagcgccg tccggcaatt tttcagagaa aacagaaaag acattccgct
    22801 gctgctgatc ctctttctgt ccggtatggc gatccgcttg tggattatca atcgtccgat
    22861 tactcacgat gaagcttata ccatcaccac atggggaagc ggaagcctga aatatgccgt
    22921 cagtgattat cagttaccga ataatcatgt ttttcacacg atcttggtaa atttaatcta
    22981 tcataccctc ggtaaaacgc cggcgctttt acgactgccg gcattgatca acgggttgct
    23041 gttgattccc gccgtttata tcctcggaaa gcagatttat aattcggcga ccgggctgct
    23101 ggcggccggg atctccgcat tttcgtcttt tttgattgac tattccgttt gggcgagagg
    23161 ttattccatt ttagcctttt gcgcggttct gattttcatc gaaggaacaa tcgttcaaaa
    23221 acaaaagaac cgttttgtct ggtgcctctt gattctcacc gcggtcatcg gattctatac
    23281 gctaccggtt atgctctatc ctttcggagc tttttgcgtt tggtttttgc tccgagccct
    23341 cttttatcgg aaatttccgc tttccggtta cggaagtatg gcaaacgcct tgaaatattt
    23401 catcgcggcg ggtatcgtaa ttgtgattct gaccgggttg tgttatttgc cgattctgcg
    23461 gaactccggc attcacgccc ttttcggaaa cgtatttgtc cggcctttgc cactcgccga
    23521 tttcaagccc actcttattt cgcgcgccat tgatttctac cgcgctttca ccgataccgt
    23581 cccgcccgtg atccggattg ctttgttcct cggaaacgtt ttggctttga tatttcatcg
    23641 aaaaatcagt cggatcttca taccgctcca agcggcattt ctgctgtggc tcttgccgtt
    23701 atgggcaatc cagcgtccca acctttggcc gcgaacctta atctttctct ggccgtttct
    23761 gatcctgtgg ggatctgccg gggtcatttg gattgtcgaa aagatatgcg catttttacc
    23821 atcgggtctt caaacaagat tcattccgct ttttatcatc gttgccgccg gtgtcaccgc
    23881 tgttccgcaa tttgatcgag ccgctgaaat tgcaaaatta agcagcccgc atgagacagc
    23941 cgttctcagg atcctggaaa atcgggacac cgaaaagatc ttatttgtgg tcgcaccgga
    24001 agatgacgcc ccgctatggt attatgccga tcgatatggc ttgccgaaat cgattttcga
    24061 taaaaatatt cctttttcga cggtctatgt ctatgtcaat ccgaaaaacg aagggttcga
    24121 ggaacctcga acccttgatg aaattatcaa tcgttatgga cccggcgaat ccttcctcga
    24181 ccgaagtacc gaaaaagtac tgatggcttc cgaaaatgcc atcctttatc gctttcatgc
    24241 gaacgagcgt gttattcgga agactttcgg cgaaactccc tgacgatccg ctgtcagctt
    24301 tttcgtttcg tttgtttggg ttgccctttc gacagagcgg gggcgcctga cttcatccga
    24361 gaaccggcga tttctcttcg aaataatttt ttctccggtt tttttgccgg ttttgcggaa
    24421 gccgattctt tcaatgcctg gacttcttca tctttcaagg caacccattc gcccggtttc
    24481 agcggaccca gcgttaacga tccgattcgg acccgaacca atttaacgat cggcaatccg
    24541 atctgtttcc cgatctcccg aatctctcgt ttacggcctt ctttcataac cacccgaatc
    24601 caagcccctt ctccttgctg gcgaatcact cgcacatcgg ccggagcggt tttgacaccg
    24661 tcctccaaca caaccccccg ccgccatttg ttcaattgat cttcatccgg acgcttggcg
    24721 actaataccc gatactcttt ctccttttca taacgaggat gagtaagttt attcgctaat
    24781 tcaccatcat ttgtgagtaa aaccaaaccc tcggattcaa aatccaacct gccgacgaca
    24841 aacagatcat cggaattcgg aaccaatgaa aagacagtcc ggcggggatc cgaagccgaa
    24901 cgatcggaca atacaaaacg gggtttatat aaagcgatat atcttttttc cggctctttg
    24961 aatttgagcg ttaccccgtc caccgccaat tgatccttga cgggatcggc ttttgcgccc
    25021 aattctacct tttcgccgtt tcgggtaact ctaccctcct taatcaaaac ttcgcaatct
    25081 ctcctggaac cataacctgc ccgtgataat atcttttgga ttcgttcttc catgtggccc
    25141 tcttctttcc cggatttccc gacaaatgtg aaattcctgc ctcaatcttt caaaatctgt
    25201 ttgtgatccg tctcttcgat cgtctcttca aacggcggca actcttcgat cgacgacaat
    25261 ccgaagtatt gcaaaaaatc ggccgtggtg gcataaagaa ccggccgccc gggggtttcg
    25321 gcgcgggcaa cctcctctat cagtcctctt gagagtaatc cacgtaccac cgactcgctg
    25381 ttaacccctc gaatcgcatc gatggcgggg cgggagaccg gctgcttgta agcgataatc
    25441 gtcaatgttt cctgtgccgc ccgagtcaat ttttgggcgg attcgattcc aaggaacttt
    25501 tctacaaagt cagccgtctc aggtgcagtg gttaattgca ctttatcatc atgaaccaat
    25561 atccgtatcc ctcgattttc agcctccaga tacccagcca gtttgcggac cagctctttg
    25621 acctcggatg gtttcatctc cgtcaccata gccagttgcg aaaacggtac gggtccggaa
    25681 gccacaaaaa ggatggattc cagaattgtc atcggttctt tgtcatcaaa agtgctgttt
    25741 tctgtcatgc tataattctc accagttcat gaataaaagg agatttaatg aatgaacaag
    25801 cataaattta ttttaccagt catctttctg ctgtgcatat tttccgccgg ttggtatgca
    25861 tacagtccta catctcagaa tgattcggga gcgaccgcgt tggaaacgct tgaaacggaa
    25921 gggaatcgca tcgctaatct ggcagcgggt acacctttca atttaacggt cagcgatgac
    25981 actttaacga gtgccgcgga agaattgatg cgtcaatata aaaatgaaat cgaaacagca
    26041 atcaaagacg ctgcgggcgt aaaactgagc ctgtccaacc cgaaagtcga attcacaaac
    26101 gacggattta atttgtcggt aaaagccgga ctcggattcc tgaaattaaa agccaaaatc
    26161 aacggaaaag tctctctggt cgacgggaaa ccgcaagtcg tggtcgaaaa gatcgacgtc
    26221 cccatcattg acatttccgc agacacggtc aataatgaga tcaagagttt gtccgaccaa
    26281 tatatgaatc agttaaccga ttcttacagt ctctccaaaa tcgagctcac agagggccag
    26341 gcggtcgttg aaggaataaa aaaatagctg aagaatgata tcaggcggtt ttttttcaaa
    26401 acaaaagcag attctccgtt ttttccaagg cgggaatttg cttttgtttt tttcgttctt
    26461 gttgattttt tcggcaaacg cctgtcaaac cgtcgacttc ctcaatcgga cgggatcagc
    26521 cgagaaaata accgtcaacg tagaaaaggg aggaaatctc ctcaccggaa cttgcgctgc
    26581 cggctcaacg gttgagcagg cgcttcagga attaaaggta aagtatcaac cggaagacat
    26641 tatcaccccc gtactgagca ctgttctcag cgaagatacc accgtcaaaa tcacctcggt
    26701 tacgtttgaa gaggaacaaa aagagcaacc gattcccttc aaaagcctga ccgttcaaaa
    26761 cgaatccatc cctgaagggg aaacttacat tatacaagcc gggcagaacg gatcggagaa
    26821 aataaccatt aaatccacct acgaagacgg agtgctcgtt tcacaggtga cttcggaaag
    26881 aaatgtaatc cgtgatgccg taccggagat tttgatgtac ggggtgaaag ccgaacatgc
    26941 ccccattcag attcccggaa agattgttta tatctcgaac ggcagcgctt ggttgctcga
    27001 gaatacaacc ggaaaccgca tcccgatcgt gaccagcgga gatctggatg gacgggtttt
    27061 ggatttatct tcggacgggg aatggctgct ctattccagg aagactcaaa acgatgccat
    27121 caattctctt tggatgctcc atataacgga ctggaatgcc aagccgatcg atttacgtat
    27181 ttccaatgtc atccactttg ccgcctggct gccgggtgaa gcgcggcgtc ttttgtattc
    27241 caccgtcaca cctcaggaaa gcgctcccgg ctggaaagcg aacaacgacc tgttgtttcg
    27301 tctggtcagc gatacgggaa tgttaatgga ggatgaaacg atcgttcaag cgaacgatga
    27361 aggaccttac agttggtggg gaaccgaatt ctatctctca aaagacggtc gcacgcttct
    27421 ctacgcaact cccggcagta tcgggaaaat tgatcgactg accggcgaga aaaaagaact
    27481 taccaagatt caaccttatg agaaaacacg cagcgattgg gcatggattc cgggagttac
    27541 ctggtcttcg gatgaaaccg ccgccttttt tgcctttcac ggggaaatag aagggacgac
    27601 aaaaagttac gatccgactg atttcaacat cgggaagatc aatcttacgg acggacaagc
    27661 cgctgaaata aaatcgggaa ccggtttgtt ttcacaaccc gcggcttccc ccctgtttga
    27721 agacggaagc tcttatatcg cttatatgca gacgatcagc cccttgcaaa gcgaaagcag
    27781 ccgttaccgc attatgctga tggattccga cggcgggaac gcaaaggcgg ttttcccgtt
    27841 ggacgaggag gattatgtca ccccgcaacg ggtcatttgg tctcccacaa cggttgcgaa
    27901 tcaatcttgg attgccatta tctataaaag caacatctgg ttggttaacc catttacagg
    27961 aatttataat caaattacaa tcgatcagtc ggtaacaaaa atgatttgga aatgatcgaa
    28021 cgagggttaa gggtatcatg agaattctaa ttacaggcgc agcgggtttt ttgggcagtc
    28081 atctttgcga tcggtttctg cgggaaggcc atgaagtgat cgggatggat aatttcatca
    28141 ctggaaatcc gcaaaatctg gcacatctgg ctgccgacca gaatttttcg ttcatccgcc
    28201 atgacgtatc gaactttatc ttcgttccca aaaaagtaga cgccgtgctt cacttcgctt
    28261 caccggccag tccgaatccg ctttctcctt acggctatac gaatctgccg attcagacca
    28321 tgaaagcggg tgcgctcgga accctgaacg cgctcggtgt ggcaaaagcg aacaatgccc
    28381 gtttcctgtt agcctccacc agcgagatct acggcatgcc cctcgaacat ccgcaaaaag
    28441 aatcctattt cggaaatgtg gatacgatcg gaatccgatc ggtttacgat gaggcgaaac
    28501 gctttgccga gtcgctcaca atggcttact atcgttttca cggcgtcgac acccgaattg
    28561 tgcgcatctt caatacctac ggaccgcgca tgcatctgga tgacggccgt gtagtgccca
    28621 atttcattca gcaagcgttg cggaaagaac agctgacagt atatggcgac ggtcagcaaa
    28681 cgcgcagttt ttgttatgtc gatgacctga tcgaagggat ctatcggctc ttcctttcgg
    28741 atgaacatta tccggtgaac atcggcaatc ccgtagaaaa caccatggtc gaactggcgg
    28801 aagaaatcaa tcgcctgacc ggtaacccgg ccggactgat ttacaaaccg gatctccggg
    28861 tcggtgacga cccgcagcga cgccaaccgg atattacacg agcgcgtgaa attttgcatt
    28921 gggaaccgaa agtgtccctc gaagaaggat taatgcggac gattccctat ttcgagcagg
    28981 caatgaaaca tgattgattt catcaataat ccaaaagaac gatcgcgttt cctgaaattc
    29041 tgtacggtag gcattatcgg agctgttgtt gatttcggcg tcatgaatct catcctgtac
    29101 tataccggcg ccaattcgag aattgccagt accatctcat tcatcgcagc cgttatcagc
    29161 aattttttct taaattattt ttgggcatat cgtgattcca gggaaaaatc aatttcgacg
    29221 aaactgttcc aattttttct ggtcagcatc atcggtttgg gaatccgata tctgttgttt
    29281 ctcacgatcg aaaatccgat catcggcttt tgcgaccgaa ttttgccggc tgattttttc
    29341 atgtccgtgg aaacagccgg gcataattta acgcttgtca tttcaatttt gatcgtattg
    29401 atctggaact ttttcgccaa ccggttctgg acatacaacg atgtttcata aaccgccatg
    29461 gccacaccga gaattatcgc cggaaccgct aagggaatgc gattacacag cgttcccgga
    29521 gacagcaccc gaccggttac cgatcgggta aaggaagcgt tattcaacat tctcggaacc
    29581 gacatcgcgg atgccggtat gttcgatctt ttcggcggga ccggttccgt cggtttggaa
    29641 gctttaagtc ggggggcatc ttacgtccga tttatggatg ccaatcgcaa cgcctataaa
    29701 acacttcagg aaaatattaa actgaccgga ttcgaaaaaa aatcgaaagc cattttcggg
    29761 gacgtattcg gctacttgcg caccgttccg gatcgtgaat tcgaatatat ctatgtcgct
    29821 cctccgcaat ataagcgcct ctggcaaaaa accatgcaga ttctggatga taacgacggt
    29881 tggttgtcgg aagacggttg gattatcgtt caaatccatc cggttgaact ggaaccgtta
    29941 gagcttaaca atttcattcg tttcgacgaa cgaaaatacg gcagtaccgc tttgctgttc
    30001 taccggcgca aaccgtcccc atcaaatgcg gcttagttac cgcacatgac gatgcaaacg
    30061 tccgattctc cgttgattga agaaaccgtc cgataatttt ccttttcgta ccgatacgcc
    30121 gttatacctg actgatttta tcgatatcat acggcgtttg ctgataaaca taataattca
    30181 gccaatttgt gaataacagg ttggcatgac tgcgccaata catgatggga gcagctttgg
    30241 gactgtttcc ggggaaataa ttttccggca tcaccggatt gattccggct ttcagatccc
    30301 gacgatactc attcgccagt gtcatcacgt catattcggc atgtccctga ataaaaatct
    30361 gtcgtccgtc gcgagtagcc gatatcgcca accccgcttc gttcgagacc gccaaaacct
    30421 gcaattccgg gtgtgcttcc acgtcttcgc gccttaccgc ggtataccgg gaatgaggca
    30481 tataaaagat ttcgtcgaaa ccacgcagca acggatgatt gcgcacaacc ggataatgag
    30541 aaaatattcc cgaaatcttc ttatccaagg gatatttgtt gatcccataa tggtaatata
    30601 atcccgcctg ggctccccag caaatatgca ttgtggaaaa cacgttcctg cgtgaccaac
    30661 tcatcagttt gcacagttct tcccaataat cgaccgcttc aaaaggaata ttttcgaccg
    30721 gcgcgccggt gatgatgaaa ccgtcatatt tattttgact gatctgatca tacgtcttat
    30781 aaaaatagtc cagataatcc tgagaagtat gtttggactc gtgcgtcacc atttgtaaga
    30841 attcgacatt gatctgtaaa ggactgttac tcaataatcg cagtaattga atttcggtct
    30901 cgattttggt cggcattaaa ttcaatatca gaatttgcaa cggccggata tcctgatgcg
    30961 ttgcccgttg ccctttcatg acaaaaatat tttcattttc cagcgtctcg gcagccggaa
    31021 gattatccgg aatattcaca ggcataatta aatcccttcc aatgcttgat tcaaatcata
    31081 cagaatatca tcgatatgtt cggtcccgat ggaaagccga acggtattca actgaatatc
    31141 ctgttctgcc aattcccgct catttaattg ggcatgggtt gtgctggcgg gatgaattac
    31201 caggcttttt acatccgcta cattcgccaa cagagaaaat acttgcaagc gatcgataaa
    31261 ttgtttggca gccttttcat cgcctttgac attgaatgtg aaaatcgaag caccgccgtg
    31321 aggaaaatat tttcggtaaa gttgatggtg gggatgatcg ggaagggacg gatggttcac
    31381 acgttccact ttcggatgag cctgcaagaa ttcgacaacc ttcagcgcat tttcgacatg
    31441 gcgttccacc ctcaggctga gtgtttccaa accctgaaga aacagccagg catgcagcgg
    31501 cgccagcgtc gcacccgtgt cccgtaacag gatcgcccgt gccctggccg caaaagccgc
    31561 cggaccggcc gcttcgacaa aactgatccc gtgataactg ggattgggtt ccgttatttc
    31621 gggaaatttt ccgctgccgg cccaatcaaa cttgccgctg tcaatgatta caccgccgat
    31681 tgccgtaccg tgaccgccga taaattttgt cgcggaatgg accacgatgt ccgctccgta
    31741 ttcgatcggc cgcaccaaat aaggtgtggc aaacgtatta tccacaatca acgggatgcg
    31801 atgagcatga gcgatttcag ccaccttttc aatatcaatc aatgtcgcat tgggatttcc
    31861 aatcgtctcg ataaaaacgg ctttggtatt gggttgtatc gctttctcga aaccgccggg
    31921 ctcatcggca tttacgaaag ttgcggaaat tccgtaatct ggaagcgtat gttgcaaaag
    31981 attataagtt ccgccgtaaa ttgtcgccgc cgagacaata tggtcgccgg cttttgccaa
    32041 attcagaaag ctgtaagtaa cggcggcagc acccgaagcc acagccaatg cagccgtacc
    32101 gttttccaaa gcagccatac gcttctcaaa gatttcctgc gtggtattgg tcattcgtcc
    32161 gtagatattc ccgctctcag ccaggctgaa ccgggcagcc gcctgatcgg tatttcgaaa
    32221 tacataggac gtcgtctgat agatcggcac cgctcgggca tccgaagcgg gatcggcctg
    32281 ctcctgcccc acatgcaatt gttttgtttc gaatttccac tcatgatttg ccgccatttt
    32341 taacctcttt cttttcgatc gaaaaccttt ccgaaaatta atataaaaaa ggacctcaat
    32401 aagcgatatc gaggtccttc tcatcttctc tgcatagccc tgactaagcg atcagatcaa
    32461 gtcacctcgc ttctgtacca tgggcatggg catcaaataa atttggtttg tcatagtaaa
    32521 aataagttta tattccccgt atcatttttg tcaatcagat tttcgaaacc ttatgacaat
    32581 tatttttcct tcttttcgat ctttggcaaa ctggcagccg cgaccgcctg cccgactctc
    32641 ctcgtttttt ccgaaggcag agatacattg agattcaaat ccggaaattc ggttcgcaag
    32701 accttgtttg ccatatccag cattctctct ccgcccttgc cggaaaccac ccgtcccagc
    32761 agaagcactt cgttcccttc gtaaaacata ctgtagagtt tcagagcgta tcccagataa
    32821 accccgatcg agtcaaaaat tttcaccgca cgcggatcat tttcctccat cagcatttga
    32881 actgttttca gtttttccgc cggcgtttcc tcttctttca aatcgatccc ggctgcgggg
    32941 cacagcttaa tgacaccatc ttggctgaag tattttacgc cgcagccctt atctcccgac
    33001 cattcgtcaa ccattgcata ctcggaaaga tcgatcggtg aaaaagcgaa ttcattcatc
    33061 caacccgtaa ttttgccttc gaggtccaca tatccaccgg cttcgctggt tcccatagca
    33121 atccccaaca ccccgttttt ctgcagactc atcgaaccgg ccaaggcggt cacatctccg
    33181 tcgttggcaa ccaccaatgg gatattttct ccgatctcac gcgcggcatc gatataaatt
    33241 gtacggacct tttttttgaa atcttccggc ggaaccttca agaacagcga ggcgactttc
    33301 gtctcgttgt tgataaaaat cccggccgaa gaaacgccga tcgcatcgac acgcggcata
    33361 tgcgcggccg cttttttaaa ggaatcgaca atcccctcgt agtgatacat cgggtcggca
    33421 tttactttcg gattccaaac cgtctcatcg ctgaaaacgg gaacgccgtc gattaccgca
    33481 gacactttgc gatcgctccc gccggcatca aacccgatcc ggcagccgtc caaatttcgc
    33541 ccgatagaaa ctgatttttg aaacggtttc gggcagtctg catactcgcg atacaacacc
    33601 tcaaacgggc ggtcataaac atcctgcata aaatgaaaat caaaactgcg cttgccgccg
    33661 gcggagtaaa cctctttgat atattcatag atttttcggc tgccgcagat tgtcagccga
    33721 aacccgctcc ttgaccagag catgaatttc accaaacgtt cggcaaataa ccggtcggcg
    33781 gcttcctttt cgggcgtatc atggataaaa gtcttataga cctgaacctg gttatcctcc
    33841 cgctcgatcg cgacggcaaa cgggacggtt gcgctccgca aaaaagattc cataaacacg
    33901 ggcaggggaa taaaatccgg atccaattcc gggctgaatt ttgtattgac gatcactccg
    33961 gctacatttt tttgcatctt gctgattcct ttatcgagag atatttttcg ttccattccg
    34021 agcggaaact attaagaatt ttatcattga cggaaagagc acgggaggga ttcggcacgg
    34081 gaagctcgcc gtttcgctca tatctgcggt tcgaaaacca acaatttctt ggttatatag
    34141 ttatcggatg acgaaccggc ccatcccgga atgacacttg gaattaagaa agatccgttg
    34201 aaccgattcc gactttttcg caacgaagtt caagcggaca gcccgtacag atcggttttt
    34261 tgcggcaatg ccgcttggca ttcgccacga tcagtgcgtg aaaatcgtta taaatctcgg
    34321 cagagggcgg aagatgctct tcaaaataag cttttatcgc ctcgtaccct ttcccggccg
    34381 caatcggata acggctgcaa agccggactg tatatgcgtc gaccacaaac gtcgggaaac
    34441 caaaagcata cagcaatatg gaatccgcgg tctcatgccc gacacctttc gtcgccagca
    34501 actcggttct gattttccgc aacggttcct tgcgaacggc ggaaacatca taaccatacc
    34561 gtgaaaacca ctccgtcacc gctttcacat agatcgcttt ctggttaaaa aaaccggcgg
    34621 gacggatgat ttccgccagc tcagtcgtgt ctgtttttgc gatcatctca ggcgagaggt
    34681 ctccgttgaa attcgacaaa gctttttcca catttcccca tgccgtgttt tgcgtcaaaa
    34741 ccgctccgac aatgacctca aaaggtgtct tcgccggcca ccaatgaagc tcgccgtaac
    34801 aggcaagcag agattcataa attgcgatca acttttcatc ttccgtcatt tcgccgatac
    34861 catcagatcg atcccgactg ttccaagtta ttgtttcgat cgaagggaat gtcaaagccg
    34921 ccgccccaaa tcagagagca gcgatcatat tctctcgatt cgtctcgctt ccccgccatt
    34981 tttgcaatgt ttcccccaac cttattgccc gtttccaatc gaattagaag aaaaatgggg
    35041 tatgttccaa atatcatgcc gcatagtttt aggatcgtca tacttgatga acggctctgc
    35101 ggtagtccta ctcatgtaaa aataccttaa tattaatttt cagccaactc ccttgaatct
    35161 ccttggcggt tattcttccg aaaagtaatt tccccgcatc cattaagcga gaaaaaatca
    35221 cattatccgc tcgcgggaca taaccgacct tcacaccatc tgcatttttg atcacgatag
    35281 cttttttatc atacggatta tccggctcac ggaaaaaatc caatttatcg ccaatattca
    35341 aatacggttc caattcttcg atgccttcga tatgacttgt cccggcgata tgggtatcaa
    35401 acagaaagat gtccctctca aaaggttggg ggacaaccgg actgccacct tcgccatgaa
    35461 acaaaccgat aataccggca ccttcgtttt ttatcagctc tcccatcatc ctacctcagc
    35521 atggcttgta ttctttctgt ataagcggct aactgttcat ttaattgtcg aatggactcc
    35581 tcaagttcgg cctttcgtgc ctcggtcttt tcgcgacttt gaatcatagg cttcaacgta
    35641 tacggatatc tcgacttcgc atcggcgatt cggtctcgga cggcttggat cagtccggta
    35701 atccgctctt tttctttgat caattccgag atcgcatccg gctcatcacc cgaaaatgca
    35761 ggtccgctga tcatcgcatg aatgacacgc aacccgttga gatcaccatt ctcatacgaa
    35821 gttacggcat tttggaacag tcgaattttt gcctcctcta tgccgggatg caggtcggga
    35881 tgcaaggctt ttacgatcgc ccgatacagt ttctttagct cacgggcttc ctcatccgat
    35941 aaaaccttcc cgtggcttcg ctcgatagcc gcgttcatct tgttgaccag ttcctccagt
    36001 ttcgcctgat attcggaaaa ttcgacatcc agaagtgtat cgatttctgc cggatccact
    36061 ttctcttggc ggtttttctt cgcctgaatc aattccgctt ttctcttcaa acgccgaatc
    36121 gcacactgaa tttcataagc cttgtattca agcccaccga tagtcagcat ataagccatt
    36181 tcaatgttct tgcactcaac aaggacaagt tcatcccgtt caaggaccag catcgataat
    36241 tccgttcgca atttttcaac ttcggatttc agcttttcaa agtccgggga tacgattacg
    36301 accgatgtct gagaattttc cgaatttacc ggatcttcct cattactctc ttctctttct
    36361 gttttatcct gccggtaacg gtcgaaatcg aaaaaagaaa gaaggtcatc ccaattttcg
    36421 ggataggttt cttcgccgtt gatctccata accgaaccgt cgttgtattg aaaggtcaga
    36481 atccattgtt cgccgtcctt aataaaatga ttattgaaat gttgcgggta agccattcgc
    36541 cagtattcca aatggagttt atccaaggca tggcggatag aggtgatttt ttctttttgg
    36601 tcagtgctcc acccgatttc atcaccaaat tcatcatgtg tcgaaaaaga cgcttcctca
    36661 tccgaaatcc gaacatagtg cgtatgaatg cccatcatat ttatacgctt gagttccact
    36721 tcggttattt ggtgagaaca tttttcagta tcgatgtctt tcaggcgttt caaccatttt
    36781 cccattacgc cggaccgcag ataattcatg atgatacctt cgacaaagtt atcattccga
    36841 aatgcggcgg taatcagccc taacacttgc ttggcgttta aatcttccac gtctttgccg
    36901 gtgattgatt tatagcttaa ttccacaccc atcttgtcca gggtttcccc atattggcaa
    36961 tccactaacc ctgaatcatg ccattccgtt tcaaactcac cataatgaaa atcctccatg
    37021 aacccgacgg tagaaccgtt cccgatttta acatcggaaa gccaaccgat atattttgtc
    37081 agcactttat atcgctcgtc acactgcgga gcgaaagcaa tccattcatc gtagatcgat
    37141 cgcagcctgt catccaaata ggtcagagct ttctctttga tctccggcgg tactccgtaa
    37201 tatgcttcgg caatcgcacc ggtgatcgct ccgatggtat cgctgtctcc gccgagagaa
    37261 atagccgtcc gaatcgcatc ctcaaaggat ctggattcaa gaaaacattc aatcgcctgc
    37321 ggtacggtct cttggcaagt ctcgttaaaa cgatatgtcg gccgaatatc gtctatttta
    37381 aaatcgagcg gataataatc gcgaacgatg cactcgcgaa tctcagattt tctcgcgcct
    37441 ttccgtgcca gataaatagc cacagcggtt gactgcgcgc cttttatacc ttccctatgg
    37501 ttatgtgtga cagccgtcac agtctcggcg agatgttcta cctcttgttt ggattcgccg
    37561 ataaaccccg ccgggctcac ccgcatcgcc gctccgtttc catagctttt atagggacgg
    37621 ggattatcgc tgaaaaccca ttgataaaat ctgccgccgt agccgcagtt ggggtattga
    37681 cgcccgattt cctgcatata ctttacgctc aagtctgaga ggaccgaata gaattcgtca
    37741 tcatagccat gaagcgatcg cggctttatt ttggaagctt ccataatcgc cttcgctacg
    37801 gcaagggtca taatactgtc atccgtagca aagcaacctt tgccaaagag ctcaaagtct
    37861 ttgctgcgat gatttttgaa ttcaaaacgc gaacctacga tatcgccgat aattgcaccg
    37921 atcatttgcc gccttctatt cttc
//
LOCUS       DAOSMM010000016        37392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1148_length_37392_cov_16.759408, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000016 DAOSMM010000000
VERSION     DAOSMM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 37392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37392
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1148_length_37392_cov_16.759408"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1617)
                     /locus_tag="PK898_04105"
     CDS             complement(175..1617)
                     /locus_tag="PK898_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU19248.1"
                     /translation="MKKNIRSKMIFSYLLIALITVIVVSLLVRWNSGQSLMNMVVEQQ
                     TANMKEAVQTYYEENGTLDGFFDRYVRNGFDSGLPAPEAVNSDSDAVAQMKPIIKPQD
                     NPQNSPDIRGLHGLVDAQYRALIPTMGYEPGEIVPENMMKDAVPVEYDGEIIAWILPD
                     TQLKFKLSNEEQLYLERTTRAIIFAGIIGVCLAILMGFLFSKRILGPVQRLTKASKAL
                     AAGNLDQTVPVTSEDEIGQLTQTFNQMSTDLVRADLQRKRLTADITHDLSTPIQIISG
                     YIEMAEDEGVEINSNRLQIIKSELQRLKRLVDDLTTLSQVEAGGLDFQFERIRPAEML
                     ERVYQTYLPILKKQNIRFVYDVSEDCPDILADEDRMIQVLHNLIENALRYTPENGTIS
                     LKAYFDEFVRIEVRDSGAGIDLEDLPYVFDRFYRADKSRNANSGKMGLGLAICKALII
                     AQKGKISAESEGIGKGTTFLILFPPYNGTD"
     gene            complement(1617..2303)
                     /locus_tag="PK898_04110"
     CDS             complement(1617..2303)
                     /locus_tag="PK898_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU19249.1"
                     /translation="MARKVLVIDDTRNIQIMLRDFLESQGFEVEIAGDGIEAFDSIER
                     SQPDLILLDIMMPNMDGYQFISQLRKTSDLPVIMITAKQQEADIVHGFELGADDYITK
                     PFKLRELLMRMRAVLRRSTPREEEKPITIIGDLQLDRSRHKVTLGDEPVELTPLEFFI
                     LDILMDSQGKVVRKEDLCIRLIENGFSGSEATLKIHIRNIRMKLRDELDQPKYIETVF
                     GIGYRFLEES"
     gene            complement(2321..2956)
                     /locus_tag="PK898_04115"
     CDS             complement(2321..2956)
                     /locus_tag="PK898_04115"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOU19250.1"
                     /translation="MNNPIMILIGFVYNLLVALILIRLIYYPKSTVQSHVFTFLAFNT
                     VIFFVINFLSSTEMGIGMGFGLFAVFSLLRYRTDPLPIREMTYLFIIVALPIMNATGI
                     ADNNWAGMIIADGLILLTIWILEKGWGFHYEISKTIIYEKIDLIVPEKYDELKADLEK
                     RTGVKINKIAIGKIDFMRDIAYLEVFSNQDPKYNWNRRMEAVTASDTDDDL"
     gene            complement(2970..3803)
                     /locus_tag="PK898_04120"
     CDS             complement(2970..3803)
                     /locus_tag="PK898_04120"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOU19251.1"
                     /translation="MNSFLRENLKLTQTMTPIVYEKKDPKRLMDEEIQTVLEKFPLIS
                     LSEMENVQLLDRIDRKYVLPIQKITDVLNDLTEDYRALFVRGRRLNHYRTLYFDTEDF
                     LLYNLHINDRKNRYKVRSREYVDSQSSFLEVKEKTNKGRTVKSRIPIDFPISQMTSEM
                     ENWVNSQIPMEDLHFEPKLWTNFTRITLVGINAPERVTLDFNLTVFNDFRAEQMNDIV
                     IAEVKTEKTDQHSPFVEKMKMMHIHPQEFSKYTIGVSEIYDGMKKNKIKPTLLKISKI
                     K"
     gene            complement(4248..4658)
                     /locus_tag="PK898_04125"
     CDS             complement(4248..4658)
                     /locus_tag="PK898_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19252.1"
                     /translation="MCKISLIYLLIAIFCALFGIIYEQFSHGVYSGYMIFAFAFPLVG
                     GTFPFAILSLFTHGLLPGRLPRFLYNAGIAALTVGSIMKGVLEIYGTTNDLLIIYRFA
                     GFGFVGTGLMIYAAELLSARKSHYEKNDSNETQQ"
     gene            complement(4675..5661)
                     /locus_tag="PK898_04130"
     CDS             complement(4675..5661)
                     /locus_tag="PK898_04130"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOU19253.1"
                     /translation="MGIFRKKYKWGVIYMILLIAFTAYVVLDTFVIARVYAKVPSVEN
                     AGIQNASESTLSANPVMESTPQATPEETAEQNNTIPFTGSAVITDNSYSDRNIRITIT
                     KYREYDTTIYVADVRVSSAEYLQTAFARGAFGRNITENTSTIAKANNAILAINGDYYG
                     AQTKGYVLKNGVLYRESASGNKEDLVIWVDGSFEIITEGEVTAQTLAEDGALQVLSFG
                     PALIMDGAIVVSENEEVGKAKASNPRTAIGIIDELHYLFVVSDGRTSTSEGLSLYQLA
                     EFMQGLGADVAYNLDGGGSSTMVFNGEVINNPTTNGNNIKERSVSDIVYIGY"
     gene            complement(6016..7968)
                     /locus_tag="PK898_04135"
     CDS             complement(6016..7968)
                     /locus_tag="PK898_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOU19254.1"
                     /translation="MKKLIHILIIFCMLAFLGSQYMAQNGNVLSVFAQTETGTVLEEE
                     DVAAEDGNEPAITSVDKVDFSDTHEDASDAEWSEADAISIQLNGDSISAENESVVIDG
                     TTVTIVTAGSYLIQGKLNDGQIVVNSEDEGPIKLILNNVEITDSKNAAIMIENADKAI
                     IILADGSTNILNDASETANEATDENRADAALYSKADLSIYGSGSLVVNGNYKEGISVK
                     DGLIIGSGDITIQAVEDGIYGKDYLVVGDGRVTVTAGKDGLKSENEDDTSQGFIEIRG
                     GEINITSDGDSISAGTDIRISGGTIHLSGGGGSSVNPNEEISGKGIKASGTIQIDGGT
                     ISVDASDDAIHSDTKVIINDGSFSLASGDDGIHADTEIEINNGQILIKQSEEGIESAV
                     ITINSGTIDITSKDDGMNASDGSGEERMGPGGSDFFGGRGMGQRPVYRGQAPENMDAV
                     QNPIAPQSPDINSGENQSASSIMLTINGGLIIVNAGGDGLDSNGSIGMSGGTVLVNGP
                     TNSGNGALDYMGTFDLSGGTVVAAGSAGMAQGSSADSSQNSIMINFDAVQEANTLFSI
                     LDQNGNVVLAFEPVKQYQSVFLTTPVLKAGETYTVRLGGTCNGTEQDHLYTDGTVIDG
                     TVYTTFEMAEGTTQIGTFAGRGMMRK"
     gene            complement(8081..10621)
                     /gene="mgtA"
                     /locus_tag="PK898_04140"
     CDS             complement(8081..10621)
                     /gene="mgtA"
                     /locus_tag="PK898_04140"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOU19255.1"
                     /translation="MEKEEIQKEIPYWNYPQEKITEMLKSDPDSGLTEAEAENRLRET
                     GLNVLKERQKLTPLDLLLNQFKSPILLILLIATVISAALGNWVDAIIIFVIILFSAVL
                     SFVQEFNADKAAEMMQMRLKIKTDVIREGKKKSVPAEVIVPGDIIELSAGSIIPADAI
                     ILRAEDFFVNQSVLTGETFPVEKTAGTSAENAELEAMNNVVFMGTTVSSGYARCIVMK
                     TGSATAFGQVAKRMVLKPPATEFERGVNKLSYLLTIVMLVLVLIIFAANIYFRRPVLD
                     SLIFSIALAVGLTPQLLPAIININLSKGSQAMAKRGVIVRRLEAIENFGSIDILCTDK
                     TGTLTEGAVTLSSALDVQGKESEDVLRSAVLNAGLQAGMVNQLDEAILKKESLPESGI
                     EKLDEIPYDFIRKRLSVLVREGDEISLIMKGSVKDVLAVCNQARMDQQTVPLTPELIH
                     QIEEKMAEWNSEGNRVLGVAVKSVREHTHNLTKEDETDMVFIGFLLFFDQPKADAIQT
                     IVDLSELGINLKIITGDNVNAAKFIANAVGMKKVSAVTGSELNNMTDEALWHIAEKSN
                     VFAEVNPNQKERIILALKKNGHVVGYMGDGINDAPSLHSADVGISVDNAVDVAKQAAD
                     FVLMKKDLTVLKDGIIEGRKTFANTLKYVFMAVSANFGNMFSMAIASLFLSFLPMLPK
                     QLLLINTLTDLPEMTIATDNVDPIYISRPHRWNITFIRRFMLVFGTLSSLFDITTFVI
                     LLGIFKADEQLFHSGWFVESIISAILVVLVLRTQQPIGKNHPSVTMLLVTILVAAIAI
                     YLPYSPIAGILGFIPIPWTAMLGIFVIAIVYFFAAEYTKRRFYQKYQP"
     gene            complement(10662..11789)
                     /locus_tag="PK898_04145"
     CDS             complement(10662..11789)
                     /locus_tag="PK898_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU19256.1"
                     /translation="MNPKIVVAGHVCVDLTPVIPHQAVEKIDQILIPGKLINTDGLSV
                     SAGGVVSNTGLALKKLGANAELMGKIGEDFLGDLLLDIFRRYGCEQGMIRSQDSATSY
                     TLVLAIPGLDRIFLHDPASNDSFCYDDLNFAHIQDATVFHFGYPPLMKRIYRNNGEEL
                     VRIFKKVKEYGVATSLDMAAIDPNSDAAKEDWQKLIREVIPYVDFFLPSMEELCSMIN
                     PPLFTEWTRRAKGKDLTEVISPKEVEPLADQLLSWGAKAVLIKCGAPGILLKTGSVSQ
                     IRQIGGNLITDPDEWANLSYFEMSYKPEKILSGTGAGDTCNAAFLLSVCRGYPWRRCL
                     QLAAGTGASCVEGYDSLSGLRSFEELEKKIDNGWQKVEWIH"
     gene            complement(11973..12599)
                     /locus_tag="PK898_04150"
     CDS             complement(11973..12599)
                     /locus_tag="PK898_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOU19257.1"
                     /translation="MKVLLINGSPHEKNCTYTALKEVADAIEKEGIETEIFHVGNKPI
                     SGCIVCDYCKRTRKNKCSIDTDSVNEALEKSKEADGFIFGSPVHFASASGAITSFMDR
                     IFRAGIYLEYKPAAAVVSCRRGGATSTFDQLNKYFTIRNMPVVSSQYWNMVHGNTPDE
                     VRQDLEGMQTMRTLGRNMAWMLKCIEAGKAAGISIPVAEKPVVTNFIR"
     gene            complement(12624..13208)
                     /locus_tag="PK898_04155"
     CDS             complement(12624..13208)
                     /locus_tag="PK898_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU19258.1"
                     /translation="MENLPSRDQAWDLLTEYISSETLQRHVLTVEGVMRHFAGLYHED
                     EEEWGIIGLLHDLDYELYPDEHCIKVQEILREKGFPEEFIHAVASHGYGICCDVEPVT
                     NAEKVLFTIDELTGLIYAAALMRPSKSVMDLETKSVMKKFKSPAFAANVNRDLIREGA
                     EKLGMPLEDVITETILGMREIAPLIGLEGEIADK"
     gene            13365..13985
                     /locus_tag="PK898_04160"
     CDS             13365..13985
                     /locus_tag="PK898_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU19259.1"
                     /translation="MQSDNIPVNLMTKKQKGFLIFKIFFSAMVFAFTGGSAATPTLIY
                     QVVKKYKLMSEDEYMEIVALSHSLPGIIGINNSMLTGYKVCGIFGSFMAVLGTALPAF
                     VSMLLVAVIFQELPQSRVVRGAINGIRAISIGILFDTGLRILIRNHKNIFGIAMIILA
                     MCLPLFTKISAFYTVLLSGLIGVVYYLTKNHNNLPAPIENKEEIDG"
     gene            13978..14547
                     /locus_tag="PK898_04165"
     CDS             13978..14547
                     /locus_tag="PK898_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU19260.1"
                     /translation="MADLWTLFFMFFKIGITSFGGGYGMMPMIMDEGQRLVGLTEAQF
                     TDMAALDLITSGPVAVNSATYIGYIKGGYAGSLVATIASILPSILICIIVLIFLDRFY
                     NNKIVKGLFAGITPACGGLMFFTTATLAKSVYFNAETFTEVFNSTVTPTIIGMIVLTI
                     LTVIADIKFKVNPIYLTLAGAVLGIFFLA"
     gene            complement(14578..17154)
                     /locus_tag="PK898_04170"
     CDS             complement(14578..17154)
                     /locus_tag="PK898_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOU19261.1"
                     /translation="MVTNQIIQWLVVLIGIGIAGFPICFSMMRYLPDKGLAFSRIAFL
                     VLFGFFYWLACSLGIIGNNAGGILIIFLPLFIFSVFLWVKNFSELYEWIRINRSYLLI
                     EEAVFLLAFSLIIVFRLANPNIAGTEKPMEMTFINGIVYSETFPPHDSWLAGYSISYY
                     YFGYVMTMLIGRLAGVPAEVSFNLMLATVFALAAAASYSLVNNLLSVARNNGFGKLKH
                     FAGSFLAPIFLLICSNLEGLLEVFYARGLFWKTDGSSAFWQWLGLKELDTAPAIAPTW
                     DITQRPGIWWWRASRVLSDTGLDGSSKEIIDEFPFFSFYLGDLHPHVLGIPFVLLALA
                     LALNAYWGYRNNGIRVSYQDQISWDKKILSVGRNFLSRTFWFDAICIGGLLFMNTWDF
                     PIYFVIYCGVYGLAAYQETGKLKKAFCAFLEKGIPFGIACVFLYFSFLSGLSSQAGGF
                     IPSGVFTTRTIQFVIMFGLFLIPIIWWLVRTVGKSHQELLRKTLKISIILFAVLFLLE
                     TIIFVGLAWFNQSGSELQSSGNKILSQLGAGMMLAGSAYAGVQGFQSMSTAVTGYFSR
                     RLQNLPLMFLIFGSLAFILAVLFSKQTNDDNDRITDEIVLSDEKKWADSSDRFVSLML
                     FLGFGLILIPEFFYLRDFFGTRMNTIFKFYYQVWILFAIASAYACSRIFEKLSSRKKW
                     LIALPMFFVTAACLIYPFQGITDNLEIVHRKVNETPSELTLDGSQFLKVSRPDDWAGI
                     DWLKKAKPGVIVEKVGASYTSDNAVSTFSGHPTILGPTNHESQWRGGYKEIGTREADV
                     QTIYESRNWTTVKKLLQGYQVRYIFIGSAEQTAYKIQEQKFRDNLETVFQSGNCRIYQ
                     VY"
     gene            complement(17164..22260)
                     /locus_tag="PK898_04175"
     CDS             complement(17164..22260)
                     /locus_tag="PK898_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOU19262.1"
                     /translation="MNLGKGDQRKPIYFLWVVPFILGIFFRFYGLNWDNGQFLHPDER
                     FLVQVADSIHFPASISLWFNAAESSFNPSNSGFHFYVYGTFPLFLIKAIQGFLGHYTD
                     PASYSFLLLTGRFLSAFFDFLTLLCICRFSRNLWKSRKAAFLSVCGYGFFVGAIQQAH
                     FFTVDSFCTFFCTFSLLSVLGDRKKHFYSQAIVSGIALGIALACKISAIYFILFPALD
                     MVFRTVRRHSDIKKELFRFFLMISASALAFRIFQPYAFQNPQFFDFRLNSSWIAAMRE
                     LAEQSSGRVDFPPAWQWVGRSPFYALKNLLIYGMGLLPGGLVFAGFVLSIRQGFKKDN
                     YVILTLIIGTAVFFLAQTAQFSKMMRYLYPICPILFLLLGSLLEVEGKVWMKIFLSTA
                     LACAFLRSCAFLSIYQRPHTRVQASRWVYDQIPAAISVQVSGETDEVEPDLQVAVPTI
                     FSVETGDDPIQIPLAVQKGDIPRLIEFENLKVNGENCSIRASINSTEENRGLILTGDL
                     IPNESQILFAGDDAILGNQSFLSLQTVGNGCSAEISGDSFLYFLRNGKPRYLLLKGLA
                     AAWITDDKPLEVHFKIQDDGSIAGLSFHSMMFSGGMDQFVPISFTFINYNNSTEKTEN
                     FSIISSANQPVAFSLSDPIKVKAGEMIGLRIAVNSKRAISVQPKRIATESSWDDRLPL
                     RMEGQIPYDPTYGRYGTVLDLDLFSSEEVLWKEKLIAKIDAADFLIIGSARGYGMADR
                     AEKKFPILNSFYRQLVSCPDGDPLTDCFNKLSVKSAKENPYFKPVAVFQSEPTLFGHS
                     LSTQSAEEAFTVYDHPKVFIFQKQPQFSLSEFRKRLDFVNLSDISDKTPVEYKNMEQT
                     YALLTESQAILQQSGGTWSELFNRSSLINSNPILTVILWYLLILGIGLIFFPLSHMIF
                     GSFRDKGYGISRFFGLLIFGYAVWVLCFSILPYTKKTILSVFLVLAGVNLLIFLFNRK
                     SILRDLYENRRYIFQSEIVFLSCFVFFLLIRLGNPDLWHPYKGGEKPMDFSYFNAVIK
                     STSFPPYDPWFAGGSMNYYYYGFFLSGLLVKWIGLIPSVAYNLILPTWYAFLGVGAFT
                     LGSNLYAHRHFGNTEFNVEKGALKTGILSVLLLQVFGNLGTLKVIAVELTELGCAGIN
                     EASTAFEKIGCFFNGIGKILQGQHFQMYAGDWYWIPSRAIPGEPITEFPFFTFLYGDP
                     HAHLFALPITVMVLVWLTSLIVRRKTSGNGFNLLSLASGALIIGSLIPTNTWDMPTYL
                     LLSCIVLLDLGVQQPLFQFNKPSSQTDSSLRKFSDQKIISSILSVVILCGLSFILFKP
                     YLDTNTRESAIDVWNGSRTPVWSYLMHWGLFLFALISWYYTETIDWLARVKVSDFRNF
                     IRKQKNVLITVAVFFVGIMVAFSAVKVWVAWIAVPLMFWTLCLLMRRDTPISQRFLLF
                     LAGTALFITLFVEFFCLRGDIGRMNMVFKLYLQAWVLFTPVSAFALISVIQRSFEKKT
                     ESEKSRKKVIWRFAYGFLLIGAFSYTIFASVDKIKDRMSTVAPHTLDGMEFMKTSFYF
                     QDGFLMDLSQDYDAIRWMQDNVKGSPVIVEANATEYKWGNRFTVYTGLPGVVGWNYHQ
                     RQQRGNMSDQVWERVNAITSFYNDSSLENAVDFLKRYNVRYIIVGQMERGMYAPEGIE
                     KFIAGNGIYWDCVYLSKDTAIYRVKEQEAK"
     gene            complement(22288..24498)
                     /locus_tag="PK898_04180"
     CDS             complement(22288..24498)
                     /locus_tag="PK898_04180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19263.1"
                     /translation="MDEMSVLDYLKLKLKPQNWHRPILPEESEFQTTETEFDEKPLRN
                     RTNLISIFADSIQRETVSHPFYRVNLSFAALMAATLLALVAQLFLEPMIVHGNRNYLL
                     SVILYGFSALILFISFFVQKKSMEEEKSDRENLLLREKEILTASLFNETFYRYWAALS
                     FGCAFIAFILFGSNRFNFINLTFWFFSVLFAVFSLKGIPSKETIKKQLIKIGSRFNPI
                     QIQLSPWNLLCILVFGLSVWFRFHQLDAVPGDMFSDHAEKLYDVMDVMDGKHSIFFVR
                     NTGREAFQFYWTVLIIKLFGTGISFLSLKIGTAIAGLFILPFVYRLGKKVGNRWVGLI
                     SMFLCGSAYWLNVIGRVALRFAFYPMFAVPALYFLIRGLSEKRRKDLVYSGIFLGIGL
                     QGYSAMRIVPLIFVVIFLIYWFKLPKNSRLNALKAFLILTGFAILFCLPLIRIATVSP
                     EMVFYRTLTRLSDAETSISASPIAIFFSNLWKALIMPFWDNGRIWVHSIPFRPALDYL
                     SASFFFIGLVLIILRIIRDKRWQDIVLILSIPLLMLPSVLSIAFPDENPCLNRTGAAA
                     IPILITAAYGIVSVGNSLISRFKESKINILFTAVLGIAFLFAIGKNNYDLVFNEYRQN
                     YDLNALNTRQIGQVIRGFADSVGSADDAYVIPYPYWVDTRLVGINAGFPRKDYALSKD
                     LIGSVVNGDSPVLFIYKDNDSETAAILKKLYPDGLEKLYEGKVSGKNFYGYLVP"
     gene            complement(24505..25323)
                     /locus_tag="PK898_04185"
     CDS             complement(24505..25323)
                     /locus_tag="PK898_04185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU19264.1"
                     /translation="MESNKEKALTDQKDYLWIQIRELPYFRGLLRSVEAKFYQDLEVK
                     APILDLGCGDGHFASRTFDFQIDVGIDPWKGPLYEAAKTGAYRNVIRCEGADIPFPDR
                     YFQTIISNSVLEHIPEVDAVVIEAGRVIKPGGTFIFCVPNHQFLSNLSVSNFFDRIHI
                     HFLANAYRSFFNKISRHHHCDSPEVWQERLDQAGFELIRYWHYFSPGAFHTLEWGHYF
                     GLPSLICHFLTGKWILFPTKWNLWLTEKICRRWYEEEAEQPDGAYTFYIAQKRS"
     gene            complement(25310..26467)
                     /locus_tag="PK898_04190"
     CDS             complement(25310..26467)
                     /locus_tag="PK898_04190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU19265.1"
                     /translation="MKILVALTYYTPYVSGLTIYADRIARAWAKRGHEVTVLTSRHDS
                     KLPEEEIVEGVRVIRAPIFMRISKGAIMPAFWRQAGKLAKESDVVNIHLPQFDAGWVA
                     RQAKRQKTFSVLTYHCDLQMPAGLINQISNQAVLRMNDIAGKNCDSIVAYTEDYANYS
                     FFLPKYMEKVRIIPPPVELPQVNEAEVKEFRMKRNPEDKSPLIGMACRFAADKGVEIL
                     LDALPKILNTYPNACVFFAGPYDNVLGERAYYERLKPRLDAMIAEGHWKFLGSLPPKE
                     MAKFYQIIDLLAMPSLNRTDAFGLVQIEAMMNGKPVVASNLPGIRVPVTRHGMGKIAE
                     IGNAEDLAQKIVEVLQEKKDYSDKTEEIRKYYEPDKIAAAYEDLFRQYGIE"
     gene            complement(26464..27492)
                     /locus_tag="PK898_04195"
     CDS             complement(26464..27492)
                     /locus_tag="PK898_04195"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU19266.1"
                     /translation="MTKKYFITGGAGFIGSNFAADLLRKNREVTIFDNLSRSGSRHNI
                     AWLRSQFGADSFRLIQGDVRDADALEKAVGNADVVAHFAAQVAVTTSVTNPREDFEIN
                     AAGTFNLLEAVRKSGRQPVVLYTSTNKVYGGMENVRVIEREEDYVYEDFPMGIAESQP
                     LDFHSPYGCSKGTGDQYVRDYSRIYDIPTVVFRQSAIYGPRQFGMEDQGWVSWFIIAV
                     LLKKPITIYGNGKQVRDLLFIDDLVRAYHAAIDAPETTAGQIFNIGGGPNNVISVWKE
                     FGPILEELCGEKIPVNYSNWRPGDQVIYVSDIRKAERLLNWRPEVSVKEGITRLYHWA
                     EENRNLFA"
     gene            complement(27485..28513)
                     /locus_tag="PK898_04200"
     CDS             complement(27485..28513)
                     /locus_tag="PK898_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU19267.1"
                     /translation="MSDQPDTKKSGIWKQMLPGILISVVIILILVFVVDFGDLIASFR
                     NVSVRLIMIVFALQSVAFIFRALAWRVTLEGIPTWAQCFWTVTEGYLLNLLPLRLGEI
                     GRAVIMGGVIHRSPFFVFSTVLLERLFDVIITLIMLLATIPLVSGADFSPNLYYVLIS
                     IFLIGLVLLFLIAKNQENFVKRLGKIIKSESKFGRFLYPKLQSLLNGLSVLAQPKKFI
                     IWLFWILMTWVCWIATMLVCMHAFFPNLPVWSSLFVQSITALGGAIPSAPAGLGVIEG
                     AFVAALRIFGINQSEALGFGMFNHSMGIITPVIWGLIGFAVQGQSFSQVFSGLRKVNL
                     SEEDAKND"
     gene            complement(28519..30318)
                     /gene="lepA"
                     /locus_tag="PK898_04205"
     CDS             complement(28519..30318)
                     /gene="lepA"
                     /locus_tag="PK898_04205"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU19268.1"
                     /translation="MKEHIRNFCIIAHVDHGKSTLADRLLQLTNTVPDRQMMDQILDS
                     MDLEREKGVTIKASAVRMNYTAKDGRDYELNLIDTPGHVDFSYEVSRALAACEGAILI
                     VDATQGIEAQTLANLYMALESDLVIIPVINKIDLPSADPDKTAKEISDLLGVDPDEIP
                     RISAKEGWNVDQVLELIVKMIPPPQGDDSKPTQALIFDSEYDSYKGVIAYVRVREGII
                     SPNMTLRMMATKADLKPVEIGIFSPAMKPVQQLTAGEVGYIATGLKTVKECRVGDTIT
                     SALEPAAEMLPGYKKAKPMVFAGIYPVEGEDYDDLRNALEKLQLNDASLDFQPETSQA
                     LNFGFRCGFLGLFHMEIVQERLEREYDLDIVVTAPSVEYQVELLNGTVITIDSPVQLP
                     DESTIKEIREPWMKLEIFSPVEFYGTIMDLARKRRGIFVSEEYPAPDRVQMNFEIPLA
                     ELIVNFFDELKSRTRGYASMDYQMYEYRPGNLVKVEVLVGGEPVDALATIVHKEDAYH
                     KGQLLVTKLKSLIPRQMFDVAIQASASGRIISRANVKALRKDVLAKCYGGDITRKRKL
                     LEKQKRGKKRMKMVGNVEIPQEAFMAILQLDEE"
     gene            30607..31410
                     /locus_tag="PK898_04210"
     CDS             30607..31410
                     /locus_tag="PK898_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU19269.1"
                     /translation="MKKSLSLSVAIFLFATLVIGIVSVQADGIKTLVVGFDQDFPPYG
                     YIGEDGNFTGYDLELAKAAADIMGLEIVYQPISWDSKDLELDSGTIDCIWNGFTINER
                     EDKYTWTEAYKGAGQVFLVSADSDIKTFDDLKDKVVAVQTDSAGLYAIQDEKNKELLD
                     SFKELLVVPQYNEAFMDLEAGAVDAIILDIDVAKFQIKGKESKYRILDEYLQKEEYGV
                     GFKLGNTELRDKVQEALNEMAKNGTFAKLSEKYFGYDSCIMAACLANEK"
     gene            31503..32156
                     /locus_tag="PK898_04215"
     CDS             31503..32156
                     /locus_tag="PK898_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOU19270.1"
                     /translation="MGLGLMLNKLSEGMATSVLIFVLTLLFSLPLGLLVALMRMSRHK
                     VLQNLMRIYISVMRGTPLMLQLLVVYFGPYYIFGIKLSPDYRFWAVIIAFVINYAAYF
                     AEIYRGGIASVPSGQYEAAKVLGYSSSQTFLKIIFPQMIKRIIPPITNEVITLIKDTS
                     LAFAVAVTEMFTVAKALASAEKTMTAYVFAGVFYYVFNLIVAFVMENIEKRLNYYRL"
     gene            32165..32929
                     /locus_tag="PK898_04220"
     CDS             32165..32929
                     /locus_tag="PK898_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOU19271.1"
                     /translation="MEIFSINDVRKSFSTQTDVLCGMSLSVQKGEVVAIIGPSGSGKS
                     TLLRCATLLETMDSGELVYEGIKAAWNGPAGNVVYAPEETLKKIKSKFGLVFQNFNLF
                     PHYSVLKNVTDAPICVQKRQKEEVFEEALTLLKKMGLSDKADAYPFQLSGGQQQRVSI
                     ARALAMNPDILFFDEPTSALDPELTGEILHVIRDLAEEKMTMVIVTHEIEFARIVANR
                     VVFMDAGVIIEEGNSDKIIRNPENERTKAFLRKLNL"
     gene            32949..33263
                     /locus_tag="PK898_04225"
     CDS             32949..33263
                     /locus_tag="PK898_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOU19272.1"
                     /translation="MYESKSKSPETDELCRAILSLKSVEECYRFFDDIFTIREIKDIS
                     QRLQVAKLLRSGAIFNEIVKKTGASTATIARVNRCLNYGADGYKMILNRLDEEEKKEI
                     KE"
     gene            complement(33349..34140)
                     /locus_tag="PK898_04230"
     CDS             complement(33349..34140)
                     /locus_tag="PK898_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOU19273.1"
                     /translation="MKAEKKRLDVLVQENGLAESRSLAQKLIMAGKVRVNDNMVIKPS
                     TLCSADDRISLDAGPRFVSRGGEKLSGALEAFGADLPNGKICADVGASTGGFTDCLLQ
                     YGAKKVYAIDVGYGILHWKIRNDPRVVSMEKTNARDLKSLPEPVELVTVDASFISLQI
                     LLPVIRNWFGDSGGTVISLIKPQFEAGKKEAAKGAGVIRDPEIHKKVLMETLGKAEEM
                     GYGIYGLIRSPLKGPKGNTEFLVWLQTVPNEFNIKEAVQNTVENG"
     gene            34324..35199
                     /locus_tag="PK898_04235"
     CDS             34324..35199
                     /locus_tag="PK898_04235"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOU19274.1"
                     /translation="MDRIKPYLKAMRINQWTKNAFIFAPLIFDGKLTHFPSFLRILIG
                     TIIFSVLYSGIYVFNDIVDIEADRLHPRKKNRPIASGTISIKNARIFSIALIASALLC
                     GGFLQSEFLLILVLVLFINIIYTFFLKRIPIIDVIAIGVLFLLRLVAGVVLITVKMFS
                     PWLYLVTFMLSLYLGFGKRRSELAALTGNSSQTRPVLNGYSLELLDQLITIVSSVTIM
                     AYSLYTFSGPTLPGNNFMMITIPIVIYGIFRYHYLIQIKHAGGAPEEVLLRDRPLQAT
                     ILIYLLAVCIGLYLY"
     gene            35209..36105
                     /gene="mvk"
                     /locus_tag="PK898_04240"
     CDS             35209..36105
                     /gene="mvk"
                     /locus_tag="PK898_04240"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOU19275.1"
                     /translation="MSEAPAKVILFGEHAVVYGEPGIAIPISALKTSAFFENNIHNAF
                     SIQSAPLGMDLNYLELADDHPLKKLIILLMNEFGITSLPEKKLRIESAIPIASGLGSG
                     AACSVAVIRAFAEEYQKSISTQRISEIAFEIEKIYHGTPSGLDNTVISFGVPIYFIKN
                     QPFQFIQIKKPLNLLIVDTEIESNTAEVVKDIRDHYATFSPLIHEIGDISRTAKTALE
                     DGDIKLIGELMNKNHDLLKRLTVSSPELDYLYEIAIEQGAIGAKLTGSGRGGNIIALV
                     EEEDQLAYLRQALQNKGAKVLN"
     gene            36102..36905
                     /locus_tag="PK898_04245"
     CDS             36102..36905
                     /locus_tag="PK898_04245"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HOU19276.1"
                     /translation="MITLLKLGGSLITNKEKPHSVRTETIRRVGFEIKEALDSDPDIK
                     LILGHGSGSFGHVPASRYHTLDGVQTSSEWRGFAEVHAEADELNKITVSILRGCGLPI
                     LGFSPLSSVFTRNRKVTRWNIDPIFQSILHGLIPLIYGDTVLDSALGGTILSTEELFA
                     ALAIKSPKPVRILLAGIEEGIWKDYQKKDSLIKEMDLSDPENTERDFIKQSAYTDVTG
                     GMFSKVQSMEELIRKNLIQEALIFSGEKQGNIKAALLGGTPGTKIFRSV"
     gene            37154..>37392
                     /locus_tag="PK898_04250"
     CDS             37154..>37392
                     /locus_tag="PK898_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19277.1"
                     /translation="MNYASALNTFRKEEVAIAPYGIRSKDSKGRQFPEEDHAFRSCFQ
                     RDKDRIIHTTSFRRLEYKTQVFINDEGDYYRTRLT"
BASE COUNT        10872 a         8630 c         7444 g        10446 t
ORIGIN      
        1 caggaattca gacaggtaag attcccgata ccatgggttg gactgtcgaa gtcgagaaga
       61 attaatcggt tattgaaaaa gccggcgata ttttccgatg tcgccggttt ttatttaatg
      121 tctcccgctt cttttccgat cctgacattt tgttgaacgt tcgtctcgtc ttatttaatc
      181 cgttccgtta tacggaggaa acaaaatgag aaaagtcgtt cctttgccga ttccttccga
      241 ttcagcagaa atttttccct tttgcgcaat gatcagcgct ttacagatcg ccaatcccaa
      301 ccccattttg ccgctgttgg catttcgtga tttgtcggca cgataaaaac gatcaaaaac
      361 atagggtaaa tcttccaaat cgattcccgc cccgctgtct cgaacttcaa ttctcacgaa
      421 ttcatcaaaa tatgctttga gtgagattgt gccattctcg ggtgtgtatc gtaaggcatt
      481 ttcaatcaaa ttatgaagga cctgaatcat ccgatcttca tcggctaaga tatcagggca
      541 atcttccgaa acatcataga cgaagcgaat attctgcttt ttcaaaatag gcagataagt
      601 ctgatagacc cgttccagca tttcggcggg acggatcctt tcaaattgaa aatccaaccc
      661 gcctgcttcc acttgagata atgtagttaa atcatctaca agccgtttca gacgctgcaa
      721 ttccgatttg atgatttgta atcggttgga gtttatctca acgccttcat cttcagccat
      781 ttcaatatat cccgaaataa tctgtatcgg tgtgcttaaa tcatgagtaa tatcagcggt
      841 cagtctcttt cgttgcaaat cggcccggac caggtctgta ctcatttgat taaaggtctg
      901 agtcaattgt ccgatttcgt cttcggaggt aacgggcacg gtttgatcaa gattccccgc
      961 cgctaaagct ttcgaagcct ttgtcaatcg ctgaacgggt cccaagattc gcttggaaaa
     1021 tagaaatccc attaagatcg ccaaacaaac accgattatt cccgcaaata tgatcgctcg
     1081 cgttgttctt tccagatata gttgctcttc attgctcagt ttaaatttga gttgtgtatc
     1141 gggtaaaatc catgcgatga tttctccgtc atattcgaca gggaccgcat ccttcatcat
     1201 gttttcggga acgatctccc ccggttcata acccattgtc ggaatcaacg cccgatattg
     1261 agcatcgacc aaaccgtgaa gaccgcgaat atcaggtgaa ttctgtgggt tgtcttgcgg
     1321 tttaatgatc ggtttcattt gcgcgaccgc atcgctgtcg gaattcaccg cttccggtgc
     1381 cggcaatccg ctgtcgaagc cgtttcggac ataccgatca aaaaagccgt ctaatgttcc
     1441 gttttcttcg tagtaagttt ggaccgcttc cttcatattg gctgtttgct gttctacaac
     1501 catattcatc aaggattgtc cggaattcca gcgaaccagc aatgaaacga caatgaccgt
     1561 gatcagggca atgagcaaat aagaaaaaat cattttgctt cgaatattct ttttcatcag
     1621 gattcttcca gaaaacgata tccgattccg aaaacagttt cgatatattt tggttgatca
     1681 agctcatccc ggagcttcat tcgaatattc cgaatatgaa tttttaacgt tgcttcggat
     1741 ccgctgaagc cgttctcgat taagcggatg cacagatctt ctttcctcac gactttgccc
     1801 tgtgaatcca tcaggatatc gagaatgaag aattctaagg gtgtcaattc gaccggctca
     1861 tcccctaaag tgactttatg gcgactgcga tccaattgaa ggtcgccgat gattgtgatc
     1921 ggtttttcct cttctcttgg agtggaacgt ctcagaaccg cccgcatacg catcagcagt
     1981 tctctcagct tgaagggttt ggtaatataa tcatccgctc cgagttcaaa accgtgaacg
     2041 atatccgctt cctgctgttt tgcggtaatc ataataaccg gcagatcgga agttttccgc
     2101 aattggctga taaattgata cccgtccata ttcggcatca tgatatcgag caaaatcagg
     2161 tccggttgtg atctctcaat cgaatcgaag gcttcgattc catcaccggc gatttccact
     2221 tcgaagccct gactttccag aaaatcacgc agcatgatct gaatattacg ggtatcatca
     2281 ataaccaaca ctttgcgtgc cacttattct ctcccaaaca tcacaaatcg tcgtcagtgt
     2341 ccgaggcggt caccgcttcc atacgtctgt tccaattgta cttcggatcc tgattcgaaa
     2401 aaacttcaag ataagcgata tctctcataa aatcgatctt cccgatagca atcttattaa
     2461 tttttacgcc ggtccttttt tcaaggtcgg cttttaattc atcgtatttt tcgggaacga
     2521 ttaaatcaat tttctcgtat atgatggttt tggaaatctc atagtgaaat ccccatccct
     2581 tttcaagaat ccaaattgtc aacagaatca gtccgtcggc gatgatcatt ccggcccaat
     2641 tattgtctgc gatccccgta gcattcataa tcggcaaagc gacaatgata aacagataag
     2701 tcatttcccg aatcggtaaa ggatcggtac gatatcgcag caatgaaaaa acggcgaaaa
     2761 gtccgaaacc catcccgatg cccatttccg tgctgcttaa aaagtttata acaaagaaaa
     2821 ttacagtatt gaatgccaaa aaggtaaaga catgtgattg tacggtggat ttcgggtaat
     2881 agatcaatcg aatcaggatc aatgccacca aaaggttata aacaaacccg atcagaatca
     2941 ttattgggtt attcataaaa ttgagcctct cattttattt tcgaaatttt caacaaggtt
     3001 ggttttattt tgttcttctt cattccgtca tatatttcac tgactcctat cgtatatttg
     3061 ctgaactcct gcggatggat atgcatcatt ttcatttttt cgacaaaagg tgaatgctga
     3121 tcggtttttt ctgtcttcac ttccgcgata acaatatcgt tcatctgttc agcgcgaaaa
     3181 tcattgaaaa ccgttagatt aaaatccagt gtgacacgtt cgggagcatt gattcccacc
     3241 aaagtaatcc gtgtgaaatt tgtccaaagc tttggttcaa agtgcagatc ttccatggga
     3301 atttgactgt taacccaatt ttccatctcg cttgtcattt gactgatcgg aaaatcaatc
     3361 ggaattcggc tcttgacggt tcttccttta tttgtttttt cttttacctc aagaaaagag
     3421 gactgactat cgacatattc tcgactgcgt actttatatc tgttttttcg atcgttgata
     3481 tgtaagttgt acaacaggaa atcttcggta tcaaaataaa gcgtccgata atgattcaac
     3541 cttctgcccc gcacaaacaa cgctcgataa tcctctgtaa gatcattcaa aacatcggtg
     3601 attttttgaa taggtagtac atatttacgg tcgattctgt ctaacaactg gacattttcc
     3661 atctcactca aactgatcag agggaatttt tccagcactg tctgtatttc ttcgtccatc
     3721 aaacgtttgg gatctttctt ctcataaacg attggtgtca tcgtttgcgt taatttaagg
     3781 ttttctctaa gaaatgagtt catatctgtt tttctccctt catcctaatt accatagata
     3841 ataatgtttc ggaaagcgga aatggtttac tttttgtata ggaataaaaa gaagtacatc
     3901 ggccttgaat cggggcattt aagaaaggaa caaccccgaa aatctgacgt ggcagtaatc
     3961 gcgtgaattt ttcagaaaac ggtgacagta tcgctcatct ccgtagattc gctctcattc
     4021 accggtgcgg ttaagccgcc caactcaatc ttatgtggca tacacgcgtt ccccgggaga
     4081 aaccacactc ttcttcgacg tatccccggt ttcattgttc attacagcgc ttgtttatcc
     4141 gccgcttccg atagtaatat gcttggacag cattgggtat tgtctgatac gcctccgtat
     4201 ccggccaagg ccggcgtcag atttatcacc ggaaccgctt tcctttttta ttgttgggtt
     4261 tcattcgaat cgttcttttc ataatgtgat tttctggctg acagcaattc ggcagcgtaa
     4321 atcatcaagc cggtcccgac aaaaccgaat ccggcaaacc ggtaaattat taatagatcg
     4381 tttgtcgttc cgtagatttc caaaacacct ttcattatgc tgccaactgt cagcgcggcg
     4441 atgccggcgt tgtacagaaa acgcggcaac ctgccgggta aaagaccatg agtaaataac
     4501 gataagattg caaatgggaa tgtccccccg actaacggga aagcaaaagc gaaaatcata
     4561 taaccggagt agacaccatg actaaattgc tcgtagatta tgccaaacaa tgcacaaaat
     4621 atcgcaatca gcaaatagat taaactgatt ttgcacatat tcctctttcc tagttcagta
     4681 tccgatataa acaatgtcac tcacactcct ttccttgatg ttgttgccgt tggttgtcgg
     4741 gttattgatg acttcaccgt taaagaccat ggttgaggat ccgccgccat ccagattata
     4801 ggcaacatca gcgccaagcc cctgcataaa ctcggcgagc tggtaaagtg aaagcccttc
     4861 gctcgtactg gtacgtccat ccgatacgac aaagagataa tgcaactcgt caataattcc
     4921 gatggcggtt ctcgggttgc tggctttcgc cttaccaacc tcctcatttt ccgaaacaac
     4981 gattgcgccg tccatgataa gcgccgggcc aaaggagaga acctgaaggg ctccatcttc
     5041 cgcaagcgtc tgtgccgtga cctcaccttc cgtgatgatt tcaaacgaac cgtctaccca
     5101 tatgaccaaa tcctccttat ttcccgatgc agattcacgg tagagcactc cgtttttcaa
     5161 aacatagcct tttgtctgag ctccataata atcaccgtta atagccagaa tcgcattgtt
     5221 agcctttgcg atcgttgaag tattctcggt gatgtttctg ccgaatgcgc cccgagcaaa
     5281 tgctgtttgc agatattccg ccgatgaaac ccggacgtcg gcgacataga tggtcgtatc
     5341 gtattcccgg tatttcgtga tagtaatacg gatgttccgg tcactatagg aattatctgt
     5401 aatcacagcc gagccggtaa acggtatcgt gttattctgc tcagcggtct cctcgggagt
     5461 tgcctgcggt gttgattcca ttaccgggtt tgctgacaaa gttgattcgg aagcgttttg
     5521 tattccggcg ttttctaccg atgggacttt agcataaacc cttgcgatga caaaggtgtc
     5581 cagcacgaca taagcggtaa acgctatcaa taaaatcatg tatatcacac cccatttata
     5641 tttttttctg aaaattccca taacatctat cctttttcgc actctgcaaa acggtgctga
     5701 gcaattcgcc aagaagcgga agaaaagcct aaagacgata accattgaaa tcatgaacac
     5761 cgaccggctt tgtatcgatc tgtttgcctc tgattttttt catcgtctcc gggatcatat
     5821 cgttcatgcc gagttgttcg acaaatacac tcggaatcgt tgttcttttt tttctccctt
     5881 catcctaatt accatagatg ataaggtctc gataagcgaa aatggtttac ttttcgtata
     5941 agaacagaaa aagaaccacc ggctttcacc ggtggttctt tttctgttta gtgcatcatg
     6001 atactcgttt tgattttatt ttcgcatcat cccccttccg gcaaaagttc cgatttgagt
     6061 tgtgccttct gccatttcaa acgttgtgta cacagtaccg tcaatgacag ttccatccgt
     6121 atacagatga tcctgttccg taccgttaca agttccgcct aaccggaccg tataagtctc
     6181 accggccttt aataccgggg tggtcaaaaa gacggattga tattgtttca ccggttcaaa
     6241 agcaaggacc acattcccgt tttgatcaag aatactgaac aaggtatttg cttcttgtac
     6301 cgcatcgaag ttaatcatga tcgaattttg agacgaatcc gcgctgcttc cctgtgccat
     6361 tccggcactg ccggcggcaa caacagtccc tccggataaa tcaaacgtcc ccatatagtc
     6421 taatgcgccg tttccgctgt tggtcggacc gttgaccaat acggttccgc cgctcatacc
     6481 gattgaacca ttcgaatcga gtccgtcacc cccggcgttg acaattatta aaccgccatt
     6541 gatcgtaagc attatggaag aggcagattg attttctccg ctatttatgt cgggtgattg
     6601 aggcgcaatc ggattttgta cagcatccat attttccgga gcctgaccac ggtaaacagg
     6661 ccgttgtccc atacctcttc cgccaaagaa atcgcttcct cccggtccca tcctttcttc
     6721 accgcttccg tccgaggcat tcatcccgtc atctttcgaa gtaatgtcaa ttgtccccga
     6781 attaatcgtt ataaccgcac tttcgattcc ctcttcggat tgtttaatca aaatttgacc
     6841 attgttgatt tcgatctcgg tatccgcatg gattccgtca tctccgctgg caagcgaaaa
     6901 actgccgtcg ttgataatga cctttgtatc ggagtgaatg gcatcgtcag aggcatccac
     6961 ggatatcgta ccaccgtcaa tttgtatcgt tcctgaggct ttaatccctt taccggaaat
     7021 ttcctcattc ggattgacgc tgcttccgcc accgccggat aaatggatcg taccgccgct
     7081 gattcttata tcggtcccgg cactgatcga atctccgtca gaagttatat taatctctcc
     7141 tccgcgaatc tcaataaatc cttgggaagt gtcgtcttcg ttttccgact tcaatccatc
     7201 ttttccggcg gtgacggtta cccgcccgtc tccgacaaca agataatctt ttccatagat
     7261 tccgtcttcg actgcctgaa tcgttatatc accgctgccg ataatcagtc catccttaac
     7321 ggaaattcct tctttataat ttccgttgac aaccaatgat ccggaaccgt atatggataa
     7381 atccgcttta ctgtaaagag ccgcgtcagc tctgttctca tctgtcgctt catttgctgt
     7441 ttcagatgca tcatttaaaa tatttgtgct cccatcagcc agaataatta ttgccttatc
     7501 ggcattttca atcatgatcg cggcattttt tgaatcggtt atttctacat tattcaaaat
     7561 caatttaatc ggtccttcat cttcggagtt tacgacaatc tgtccgtcat tcagctttcc
     7621 ctgaatcaga taacttccgg cggtaacaat tgtcactgtc gttccgtcga tcaccacact
     7681 ttcgttttca gccgaaatgg aatctccgtt taattgaatg gatatggcat cagcttcact
     7741 ccattccgca tcggaagcat cctcatgcgt atcggaaaaa tcaaccttat caacggaagt
     7801 tattgccggt tcgtttccat cctctgccgc gacatcctct tcttcaagaa cggtgccggt
     7861 ttcggtttgt gcaaatacag aaagaacgtt tccattttgt gccatatatt gactgccaag
     7921 aaaagccaac atacagaaaa taatcaagat atgaatcaat tttttcatac tcctccaagt
     7981 ttattttttc tttgattcta caaattaaat aaaaagaatc ggtttattta tggtatagac
     8041 cgattctttc ttaaattttc aataacaaac aagaaaatcg ctacggttga tacttttgat
     8101 aaaatcttcg tttggtatat tcggctgcaa aaaagtaaac aatcgcgatt acgaaaattc
     8161 ccaacattgc cgtccacggg atcggaataa agcccaaaat acccgctatc ggagagtaag
     8221 gcaaataaat ggcaatcgcc gcgactaata tcgttaccag caacatcgtg acagaaggat
     8281 gatttttccc gatcggttgc tgagttctca gaaccaagac caccaaaatt gcggaaataa
     8341 tcgattcgac aaaccatccc gaatgaaaga gctgctcatc agccttgaaa atacccaaca
     8401 ggatgacgaa ggttgttata tcaaacaaag aagaaagagt cccgaatacc aacataaatc
     8461 gtcggataaa agtaatattc cagcgatgcg gtcgacttat gtaaatcgga tcgacattat
     8521 ccgttgcaat agtcatttcg ggtaaatccg taagcgtatt aatcaaaagc aactgcttcg
     8581 gcagcatcgg taagaaggat aaaaataggg aagcgatagc catactgaac atattgccga
     8641 aattagcgct cacggccata aatacatatt tcaatgtatt ggcaaaagtc tttctccctt
     8701 cgatgattcc gtctttcaaa accgtcaaat ccttcttcat caggacaaaa tcggctgctt
     8761 gctttgcgac atcgactgca ttatctaccg atatccccac gtccgcgcta tgcaatgagg
     8821 gcgcatcatt aataccgtcc cccatataac cgaccacatg tccgttcttt ttcaacgcca
     8881 aaataattcg ttctttttga ttgggattca cttcggcaaa cacattggat ttttcggcga
     8941 tatgccaaag agcttcatcc gtcatattat tcaattccga gccggtcacg gcactgactt
     9001 ttttcattcc tacagcattg gcaataaact tcgcggcatt aacattatct ccggtaataa
     9061 ttttcaaatt aatccccaac tcggagagat cgacgatggt ttgaatcgca tcggctttcg
     9121 gttgatcaaa aaataagaga aaaccgataa aaaccatgtc tgtttcatct tcttttgtca
     9181 agttatgggt gtgttccctg actgatttaa ccgctacacc caagactcga ttcccttcgg
     9241 aattccattc ggccatcttt tcttcgattt gatgaatcag ctccggtgtc agcggaaccg
     9301 tctgttgatc catacgagct tgattgcaga cagccaaaac atctttaacc gatcctttca
     9361 tgatcaaaga aatttcatcg ccttcgcgaa ccaaaacgct caaacgctta cggataaaat
     9421 catatggaat ctcatctaat ttttcaattc cggattccgg cagactttct tttttcaaaa
     9481 ttgcttcatc caattggttc accattccgg cttgtaatcc ggcattcaat acggccgatc
     9541 gaaggacatc ttccgattct tttccctgta cgtccagagc gctgcttaac gtaaccgcac
     9601 cttcggtcag tgtccccgtt ttatccgtgc aaagaatatc gatgctgccg aagttttcaa
     9661 tagcttccag ccggcgtacg atgacacccc tttttgccat tgcttgagaa cctttggaga
     9721 gattaatatt gataatcgca ggtaagagct gcggtgtcaa tccgacggct aaggcgatcg
     9781 aaaaaattaa cgaatcgaga accggacggc gaaaataaat atttgcggca aaaatgatca
     9841 acactaaaac caacatcaca atggtcaaca ggtaactgag cttattgaca ccacgctcga
     9901 actcggttgc gggtggcttc agaaccattc gtttggcgac ctgcccgaaa gccgttgccg
     9961 accctgtctt cataacgatg catctggcat atccggaact gaccgtcgta cccataaaaa
    10021 cgacattatt catcgcttcc aactcggcat tttcagccga tgttcccgct gttttttcaa
    10081 caggaaaagt ttcgccggtt aatacggatt ggttcacaaa gaaatcttcc gctcggagga
    10141 taatcgcatc tgcgggaata atactgccgg ctgaaagctc gatgatatct ccgggcacaa
    10201 tcacttctgc cgggacagac tttttcttac cctcccgaat gacatcggtc ttgattttta
    10261 atcgcatttg catcatttca gccgctttat cggcattgaa ttcctggaca aaagagagaa
    10321 ctgcgctgaa caagataatc acaaagataa tgattgcgtc cacccagttc cctaatgccg
    10381 cggaaataac ggtcgcgatt aataaaatca acaaaatagg acttttaaac tgattaagaa
    10441 ggagatccaa cggggttaat ttttgcctct ctttgagaac attcaatccc gtttctctta
    10501 atcggttttc ggcttcagcc tccgtcaacc ccgaatcagg atcacttttt aacatttcgg
    10561 tgattttttc ctgcgggtaa ttccaataag gaatttcttt ttgaatttct tctttttcca
    10621 ttttcgatac ctcccttttg tgttctcgga ttaaactctg tttaatggat ccattcgacc
    10681 ttctgccaac cgttatcaat ctttttttcc agttcctcaa aagatctcag accgctgagt
    10741 gaatcatatc cttcgacaca agaagcaccc gtcccggcgg ccaactgcaa acagcgtcgc
    10801 cagggatatc cgcggcaaac cgacagcaaa aaagccgcat tacaggtatc tcccgctccc
    10861 gtgcccgaga ggattttttc cggtttgtag ctcatttcga aataagacaa attcgcccat
    10921 tcgtccggat cggtgatcag gtttcctccg atttgtctga tttgagaaac agaaccggtt
    10981 tttaataaaa ttccgggtgc accgcacttg attaagaccg cttttgctcc ccatgacagc
    11041 aattgatccg ccaacggttc aacctctttc ggcgatatca cctcagtcaa atctttaccc
    11101 ttcgcccgtc tcgtccattc cgtaaaaagc ggtggattga tcatgctgca caattcttcc
    11161 atacttggca aaaagaaatc cacatatggg ataacctccc gaatcaactt ttgccaatcc
    11221 tcttttgcgg catcggaatt cggatcgata gctgccatat ccaatgacgt ggcgacaccg
    11281 tattctttaa ctttcttaaa tatccgaacc agttcttcgc cgttattccg gtaaatacgc
    11341 ttcatcaacg gagggtatcc gaaatgaaaa acagttgcat cttgaatatg cgcaaaattc
    11401 aaatcatcat aacaaaaaga atcattggaa gcgggatcat gaagaaaaat tctgtctaat
    11461 ccgggaattg ccaaaacaag cgtgtaagag gttgccgaat cctgcgaacg aatcatgcct
    11521 tgttcgcatc cgtaccgccg aaaaatatcc agcagcaggt ctcccaaaaa atcctctccg
    11581 attttcccca tcagctccgc attcgcgcct aattttttca aagctaaacc ggtattggag
    11641 accacaccgc ctgccgatac agataatccg tcggtattga ttaatttccc cggtatgaga
    11701 atttgatcga ttttttcgac cgcttgatga gggataaccg gtgtgagatc tacacaaaca
    11761 tggccggcta caacgatttt tggattcatt tatgctccga ttggttattc cgaataggta
    11821 aataatctac aaaacaatta tatctgctga acgacaagcc aagcaacaat tcaagttgag
    11881 cgggtttacg aaaaatgaaa cccatcgaat gaaattaaaa taagagaacc gccatcgttt
    11941 tggatggcgg ctctcttcgt ttccataaga catcagcgga taaaatttgt cacgacgggt
    12001 ttttccgcta caggaatcga gataccggcg gcttttccgg cttcgataca tttcaacatc
    12061 caagccatat ttctaccgag agtacgcatt gtctgcattc cttccagatc ttgccgtact
    12121 tcatccggcg tatttccatg gaccatgttc caatattgcg atgagacgac cggcatgttt
    12181 cgaatcgtaa aatatttatt cagttgatca aacgttgagg ttgcgcctcc tctgcggcag
    12241 ctcacaaccg ctgcagccgg tttatattcc aaataaatgc cggcacgaaa tattcgatcc
    12301 ataaaactgg taatagctcc ggatgccgaa gcaaagtgga cgggagagcc aaagataaat
    12361 ccgtcggctt ccttcgattt ctccaaagct tcattgaccg aatcggtatc gatggaacac
    12421 ttgttttttc ttgttcgctt acaataatcg cagacaatgc aaccgctgat cggtttattc
    12481 ccgacatgaa atatctcggt ttcaattcct tccttttcaa tcgcatcagc gacttccttc
    12541 aaagccgtat aagtgcaatt cttttcgtga ggacttccat taatcagcaa tactttcatc
    12601 ttggtttact ctccttttct cctttattta tcggcaattt ccccttccaa accgatcaat
    12661 ggggcaattt ctcgcattcc caaaatcgtc tcggtaatca catcttccaa tggcattccc
    12721 aatttttccg ccccttcgcg aatgagatcc cgattgacgt tggccgcaaa tgcgggagat
    12781 ttaaactttt tcataaccga ttttgtctca aggtccatca cacttttaga tgggcgcatt
    12841 aaagcggccg cataaatcaa accggtcagt tcatcaattg taaataacac tttttcggca
    12901 ttcgtgaccg gctcgacatc gcaacaaatc ccataaccat gacttgcaac cgcatgaata
    12961 aactcttccg ggaaaccttt ttctctcagg atctcctgaa cttttataca atgttcatcg
    13021 ggatacagct cataatccaa atcatgcaac aatccgataa ttccccattc ctcttcgtct
    13081 tcatggtaaa gtccggcaaa atgacgcatc acaccttcaa ccgttaaaac atgcctctgt
    13141 aatgtttcgc tcgaaatata ttccgtcaat aagtcccaag cctggtccct ggaaggaaga
    13201 ttctccataa tctttattct gtcctctgct ttctgtaaat ctttttctat tatacattca
    13261 gatttatgtg aatttttatg tcctgtaatt ggagttgaaa caagaaaatt atgttcggaa
    13321 tgttataatc ggttcgacca aaagatagaa attgggggta attcatgcaa tcagataata
    13381 ttcccgtaaa tttaatgaca aaaaagcaaa agggattcct gattttcaaa atttttttca
    13441 gcgcgatggt attcgctttt acgggaggaa gcgcagccac acccacctta atttatcagg
    13501 ttgttaaaaa atataaactg atgagcgaag atgaatatat ggaaatcgtc gcgctcagcc
    13561 attcgttgcc cggaattatc ggaatcaaca acagtatgtt aaccggttat aaagtctgtg
    13621 gcatattcgg atcttttatg gcggttctgg gaaccgcttt accggccttc gtgtcaatgc
    13681 ttttagtggc ggttatcttt caagaacttc cgcaatcgag agtcgtcaga ggtgcgatta
    13741 acggtatccg agccatttcg atcgggatct tatttgatac cggtcttcga atattaattc
    13801 ggaatcataa gaacattttc ggaatcgcca tgattattct ggcaatgtgt ttgcctttat
    13861 ttacaaaaat cagtgctttt tatacggttc tgctgagcgg gctgatcggg gtggtctatt
    13921 acctgacaaa aaatcataac aatcttccgg ctccgattga aaataaggag gaaatcgatg
    13981 gctgatcttt ggacgctttt ctttatgttc ttcaaaatag ggatcacttc tttcggcggc
    14041 ggatatggga tgatgccgat gattatggat gaaggacaac gtttggtggg gttgaccgaa
    14101 gctcagttta ccgatatggc ggccttggat ttaataactt ccggaccggt ggctgtcaat
    14161 tccgcaactt atatcggcta tatcaaaggc ggttatgccg gctcgctggt cgcgacgatt
    14221 gcctcgattt tgccgtcaat attgatctgt attattgtcc taattttcct ggacaggttt
    14281 tataacaata agattgtaaa aggtcttttc gcgggaatca caccggcatg cggcgggttg
    14341 atgtttttta cgacagccac actcgccaaa tccgtatatt tcaatgcgga aacttttacg
    14401 gaggtgttca attcaaccgt cacaccgacg attatcggga tgatcgtttt gacgatatta
    14461 accgtcatag cggatattaa attcaaagtg aatcctattt atctgactct tgccggagct
    14521 gttttaggaa ttttctttct cgcgtgattc gtccaagccc gcttgaatat gaaaacctta
    14581 ataaacctga taaattctgc aattcccgga ttgaaagacc gtttctaaat tatcccggaa
    14641 tttttgttcc tgtattttgt atgcggtttg ttcggccgat ccgataaaaa tatatcgaac
    14701 ttgatagccc tgcaacagtt tttttaccgt tgtccaattt cttgattcgt atattgtttg
    14761 aacatccgct tcccgggttc cgatttcttt atacccgccc cgccattgac tctcatgatt
    14821 ggtgggacct aaaattgtcg gatgaccgga aaaagtactt acagcattat cggaggtata
    14881 actcgcacct actttttcaa caatcacacc gggtttcgct tttttaagcc aatcgatccc
    14941 tgcccaatca tccggtcgtg agaccttcag aaactgcgat ccgtccaatg ttaactccga
    15001 aggagtctcg tttactttcc ggtgaacgat ttccagatta tccgtaattc cttggaaagg
    15061 ataaatcaag caggcagctg tgacgaagaa catagggaga gcgatcagcc attttttcct
    15121 cgaagataat ttttcaaaaa tccggctgca ggcgtaagcg gaagcgatag cgaacaatat
    15181 ccaaacctga taataaaact tgaaaatcgt attcatgcgt gtcccaaaaa agtcacgcag
    15241 gtagaagaat tcgggaatca atatcaatcc gaaccccaag aaaagcatta atgaaacaaa
    15301 tcgatccgaa gagtccgccc attttttttc atcgcttagg acaatttcat ccgtgatccg
    15361 gtcgttgtcg tcattcgttt gtttggaaaa taaaacggcc agaataaaag ccaaggaccc
    15421 gaatataaga aacatcaacg gaagattttg caatcttctc gaaaagtaac cggtaacggc
    15481 agtactcatg gactgaaagc cttgtacgcc ggcataagcg gaaccggcta acatcattcc
    15541 ggctcccaat tgagagagga tcttatttcc ggaggactgt aattcggaac cggattgatt
    15601 gaaccaagct aacccgacaa aaatgattgt ctccagcaaa aaaagtacag caaacagaat
    15661 aatacttatt tttagcgtct tcctcaataa ttcctgatgg ctttttccta ccgttctaac
    15721 caaccaccag ataatcggga tgagaaacaa accgaacata atcacaaact gaatcgttcg
    15781 tgtcgtaaaa acgccgctgg gaatgaatcc cccggcttga gaggataagc cggataaaaa
    15841 gctgaaatat aaaaaaacac aggcaattcc aaaaggaatc cctttttcaa gaaatgcaca
    15901 gaaagccttc tttaattttc cggtttcctg gtaggcggcc aacccgtaaa cgccgcaata
    15961 aatgacgaaa taaatcggaa aatcccaagt attcatgaac agcaacccgc cgatacagat
    16021 tgcatcaaac cagaatgtcc gtgataaaaa gttccgcccg acgcttaata tctttttatc
    16081 ccaagaaatt tggtcctgat aagaaactcg gatcccgtta ttacgatatc cccaatatgc
    16141 gtttaaagct aatgccagcg ccaataagac aaatgggatg ccaagaacat gaggatgaag
    16201 atcgcctaaa taaaatgaaa aaaagggaaa ctcatcaata atttctttgc ttgaaccatc
    16261 caaacctgta tcggataaga cccgtgaagc gcgccaccac caaattcccg gccgttgggt
    16321 aatgtcccaa gtcggcgcga tggctggtgc ggtatccaat tcctttaaac ctaaccattg
    16381 ccaaaaagcg gatgaaccat ctgttttcca aaaaagtccg cgagcataaa acacttccaa
    16441 taatccctca aggttactgc aaatcagcag aaaaattggg gccaaaaaag agccggcaaa
    16501 atgtttcaat ttaccgaatc cgttatttct ggcgacggaa agcagattat ttaccaaaga
    16561 ataggaagcc gcggcggcta atgcaaagac ggttgccagc atcagattaa aactgacttc
    16621 tgcgggaact ccggcaagtc gaccgatcaa catcgtcatg acatatccga agtaataata
    16681 cgaaatactg tatccggcca accaggaatc atgcgggggg aaggtctccg aataaacaat
    16741 tccattaata aaagtcattt ccatgggctt ttcagtgccg gcaatattgg gattcgccaa
    16801 gcgaaataca ataatcagcg aaaaagcaag taaaaaaaca gcttcttcaa tcaacaggta
    16861 agatcgatta attctgatcc attcatacag ttcggaaaag ttttttaccc ataagaaaac
    16921 ggaaaaaata aacaaaggaa gaaaaataat gaggattccg cccgcattat tcccgatgat
    16981 gcccaacgaa catgccaacc aataaaagaa tccgaatagt accaaaaaag cgatccggct
    17041 gaatgccaac cctttgtccg gtaaatatcg catcatagaa aaacaaatcg gaaatcccgc
    17101 gattccgatc ccgattaata cgacaagcca ttggattatt tgatttgtaa ccataatgat
    17161 cgactatttc gcttcctgtt cttttacccg ataaatcgcg gtgtctttgg atagataaac
    17221 acaatcccaa taaattccgt ttccggcaat gaatttttca attccttccg gagcatacat
    17281 tccccgttcc atttgtccga cgataatata cctgacgttg tatcgtttca aaaaatcgac
    17341 cgcattttca agcgatgaat cattatagaa ggaagtgatc gcatttaccc gttcccaaac
    17401 ctgatcggac atattaccgc gctgttggcg ttggtgataa ttccaaccaa cgactccggg
    17461 taagccggtg tataccgtaa aacgattgcc ccatttatat tctgtcgcat tcgcttctac
    17521 gattaccggc gatcctttta cgttatcttg catccatcgg atagcatcat aatcctgaga
    17581 caaatccatc aaaaaaccat cttgaaaata aaaagatgtt ttcataaatt ccataccgtc
    17641 caatgtatgg ggtgccacgg ttgacattct gtctttgatt ttatcaacgg aagcaaaaat
    17701 cgtataactg aaggcgccga ttaatagaaa cccgtaagca aaccgccaga tcactttttt
    17761 ccggcttttc tcggattccg tcttcttttc aaaactcctt tgaatcacac tgatcagcgc
    17821 aaatgcagat accggcgtaa ataataccca agcttgcaaa taaagcttga ataccatgtt
    17881 catccggccg atatcccctc tgagacagaa gaactcgaca aacaatgtga taaacaaagc
    17941 ggtcccggca aggaataaca ggaagcgttg gctgatcgga gtatccctcc tcattaataa
    18001 acataacgtc cagaacatca acgggacggc aatccatgct acccatactt ttaccgcgga
    18061 aaatgcaacc attattccga caaaaaatac cgcaacggtt atcaaaacat ttttttgttt
    18121 tcgaatgaaa tttcgaaaat cactgacttt tacccgagct aaccaatcaa tcgtttccgt
    18181 ataataccaa ctgatgagtg caaagaggaa taatccccaa tgcatcaaat aagaccagac
    18241 gggtgtccga ctgccattcc atacatcgat cgcggattct cgagtatttg tatccaaata
    18301 gggtttaaac agaataaaag acagcccgca taaaattact accgatagga tcgaagaaat
    18361 aattttttga tcggagaatt tccgcagcga tgagtctgtc tgcgatgatg gtttattgaa
    18421 ttgaaaaagc ggttgctgca ctcccaaatc caataataca atgcaggaga gcaataaata
    18481 agtgggcatg tcccaagtat tggtcgggat taatgacccg atgatcaatg cgccgcttgc
    18541 aagagataac agattgaaac cattgccgga cgtttttctg cggacgatca gcgaagtcaa
    18601 ccaaaccaat accatgaccg tgatcggtaa ggcaaaaaga tgggcgtgag gatctccata
    18661 tagaaaagta aaaaaaggaa attcggtaat cggttcgccc ggtatggcac ggctcggaat
    18721 ccagtaccaa tcccccgcat acatttggaa atgttgcccc tgaaggatct tcccgattcc
    18781 gttgaaaaag caaccgattt tctcaaaagc tgtcgaagct tcattaattc ccgcacatcc
    18841 cagctcggtc aattccaccg cgatcacttt gagtgtccct aaattcccga aaacttgtaa
    18901 taataaaacc gataaaatgc cggttttcag ggctcctttt tcgacattaa attcggtatt
    18961 tccgaaatgt cgatgcgcgt aaaggttgct tcccaacgta aaggctccaa ctcccaaaaa
    19021 tgcataccac gtcgggagaa tcagattgta ggcaacactc ggaatcaatc cgatccattt
    19081 caccaacagc cctgacagga aaaaaccata ataataataa ttcatcgatc cgccggcaaa
    19141 ccacggatca tacggaggga acgaagtact ttttatcacg gcgttgaagt atgaaaaatc
    19201 catgggcttc tcgccccctt tataggggtg ccacaaatcc ggatttccca ggcgaatcaa
    19261 taaaaagaaa acaaaacaac ttaaaaagac gatttcggat tgaaaaatat accgccgatt
    19321 ttcgtaaaga tcgcgcaaga ttgatttccg attaaataaa aagatcagta gattgactcc
    19381 ggcaagaacc agaaaaaccg aaagaatggt tttttttgta tatggaagga ttgaaaagca
    19441 tagcacccat accgcatatc cgaagatcaa taaaccaaag aagcgggaaa ttccgtatcc
    19501 tttatcccga aaagaaccga agatcatatg agacaaaggg aaaaaaatca atccgattcc
    19561 caaaattaat aaataccaaa gaataacggt caaaatcgga ttggaattaa tcagtgacga
    19621 acggttgaac aattcggacc atgtcccgcc ggattgctgt aagattgctt gagattcggt
    19681 taaaagcgca tatgtctgtt ccatattttt gtattctact ggagttttgt cggaaatatc
    19741 ggaaagattg acaaaatcca aacgtttccg aaattcggac aaagaaaatt gcggctgttt
    19801 ttgaaagata aatactttcg gatgatcata caccgtgaac gcttcttcgg cagattgtgt
    19861 ggataatgag tgaccgaata gagtcggttc agactgaaat acggcaaccg gtttaaagta
    19921 cgggttctct tttgcgcttt tgacggataa tttattaaaa caatcggtta aagggtcacc
    19981 atccgggcaa gataccagtt gacgataaaa ggaattcaat atcggaaact tcttttcagc
    20041 ccgatccgcc atcccataac cgcgagcgga accgataatc aaaaagtccg ccgcatcgat
    20101 tttcgcaatc aacttttctt tccataaaac ttcttcggat gagaagagat ccaaatccag
    20161 cacggttccg taccgtccgt aagtcggatc atagggaatc tgtccttcca tccgcaaagg
    20221 aagccggtca tcccagctgc tttcggttgc aattcgtttc ggttgaacgg atatagctcg
    20281 ttttgaattt acggctatac gcaatcctat catctcacct gcttttactt tgatcggatc
    20341 ggaaagtgaa aaagcaaccg gttgattcgc ggaactgatg attgaaaagt tttccgtttt
    20401 ttcggtggaa ttgttgtaat ttataaaagt gaacgaaatc ggcacgaatt gatccattcc
    20461 gccactgaac atcatcgaat gaaagctcaa accggcaatt gaaccgtcgt cttgtatctt
    20521 aaaatgaact tccaacggtt tgtcatcggt tatccacgca gcagccagcc ctttcaataa
    20581 caaataacgg ggtttgccgt tccgaagaaa ataaagaaaa gaatccccgc ttatctcggc
    20641 agaacagccg ttccctaccg tttgcaatga aagaaaagat tgattgccga gaatcgcgtc
    20701 atccccggca aataggattt gtgattcatt cgggatcaaa tcacccgtca aaataagtcc
    20761 ccgattttct tccgtactat tgatcgaagc gcggatcgaa cagttttcac cgttcacttt
    20821 taaattttcg aactctatta atcgaggaat gtcccctttt tgaaccgcca atggaatttg
    20881 gattgggtca tcccctgttt caacagaaaa aatcgtcggt accgcaactt gcaaatccgg
    20941 ttcaacttca tccgtctctc ccgagacttg aacggatatc gcagccggaa tttgatcata
    21001 gacccaccgg cttgcctgaa cacgggtatg gggacgctga taaattgaaa ggaaggcaca
    21061 tgatcggaga aaagcgcatg cgagagctgt cgataggaaa atcttcatcc agactttccc
    21121 ttcaacttcg agaagagatc cgagaagcag aaacaaaatc ggacatatgg ggtaaagata
    21181 acgcatcatc tttgaaaatt gagcggtttg cgccaagaaa aaaaccgctg ttccgatgat
    21241 gagcgtcaat atgacgtaat tgtctttttt gaatccctga cggatagaca gaacaaagcc
    21301 ggcgaaaacc aatccccctg gtaataatcc catcccataa atcaacagat tcttcagggc
    21361 ataaaacggt gatcttccca cccattgcca tgcaggcgga aaatcgactc tgccgctgct
    21421 ttgttcagcc aattcccgca ttgcggcgat ccacgatgaa tttaaccgaa aatcgaagaa
    21481 ttgcggattc tgaaaagcat aaggctgaaa aatcctgaaa gcaagagcgg aagctgaaat
    21541 catcaaaaag aatcgaaaca attccttttt gatatcgcta tgcctccgaa ccgtccggaa
    21601 gaccatatca agcgcgggaa acagaataaa ataaatcgct gatattttac aagccagcgc
    21661 gatccccaat gcaattccgc tgacaatggc ttgcgaatag aaatgcttct ttcgatcacc
    21721 caaaacagaa agaagagaga aagtgcaaaa gaaagtgcaa aaagaatcaa ccgtaaaaaa
    21781 atgtgcctgt tggatcgctc cgacaaaaaa accgtacccg caaacgctta agaaagccgc
    21841 cttacgactt ttccatagat tccggctgaa tcggcagatg cacaataacg tcaaaaaatc
    21901 aaaaaaagcg ctaagaaacc ttcctgtcaa caacaaaaat gaataggagg ccggatccgt
    21961 gtaatgaccg aggaatcctt gaatggcttt aatcaaaaac agcggaaaag ttccataaac
    22021 ataaaaatga aaaccgctgt tggaaggatt aaaagaggat tccgcggcat tgaaccagag
    22081 cgaaatcgaa gccggaaaat gaatgctgtc cgctacttgt actaaaaaac gttcatccgg
    22141 atgaagaaat tgaccgttat cccaatttaa cccgtaaaat ctgaaaaata tgccgagtat
    22201 gaacgggaca acccacagaa aatagatggg ctttctttga tcgccctttc cgaggttcat
    22261 acgaatctct tccggttatt caagcctcta cggaaccaaa taaccgtaaa aattcttacc
    22321 ggagactttt ccctcatata atttttccaa accgtccgga tagagctttt tcaaaattgc
    22381 tgccgtctca gaatcattgt ctttataaat aaacagcacc ggcgaatccc cattaacgac
    22441 cgaaccgatc aaatctttac ttaatgcata atcttttcgc gggaatccgg cattaattcc
    22501 caccaacctt gtatcgaccc aataaggata agggatgaca tatgcatcgt ctgctgagcc
    22561 gaccgagtcc gcaaatcccc tgataacctg cccgatttga cgggtattta aagcatttaa
    22621 atcataattt tgccgatact cattgaaaac caaatcataa ttattcttcc cgatagcaaa
    22681 gagaaaagcg attccgagca ccgccgtaaa aaggatattt attttgcttt ccttaaaacg
    22741 cgaaatcaat gaattgccaa ccgaaacgat cccataggca gccgttatca aaatcggtat
    22801 tgctgccgct ccggttcgat ttaaacaggg gttttcatcc ggaaacgcga tagacaaaac
    22861 ggaaggcaac atcaacagcg gaatagaaag gatcaacacg atgtcctgcc atcgcttatc
    22921 ccggataatt cgcagaatga ttaagacgag tccgataaag aaaaaagaag cgcttaaata
    22981 gtccagcgcc gggcgaaatg ggatcgaatg aacccagatc ctgccgttat cccaaaaagg
    23041 cattatcagt gctttccaca gatttgaaaa aaagatagcg ataggtgaag cactgatcga
    23101 tgtttctgcg tcactgagtc tggtaagtgt tcgataaaaa accatttcag gagaaaccgt
    23161 tgctatgcga attagcggta agcagaaaag aatcgcaaat cccgtcaata tcaaaaaggc
    23221 ttttaatgca ttcaaacggg aatttttggg aagtttgaac caataaatca ggaagatcac
    23281 aacaaatatt aacggaacaa tccgcatcgc actgtatccc tgcaagccga tcccgaggaa
    23341 aattccgctg taaaccagat cttttcttct tttttcggac aaaccgcgaa taagaaaata
    23401 aagtgccggt accgcaaaca tcgggtaaaa agcaaatcgc agcgctactc gaccgataac
    23461 attcagccaa taagcgctcc cgcataaaaa catggaaatc aatccgaccc aacggtttcc
    23521 gactttctta ccgagccggt aaacaaaagg caaaataaat aagcccgcta tcgctgtacc
    23581 gattttcaaa ctcaaaaagc tgattcccgt accgaaaagt ttgatgatca gtaccgtcca
    23641 ataaaattga aatgcttcgc gtccggtatt tctcacaaaa aagatggaat gttttccgtc
    23701 catcacatcc atcacgtcgt aaagcttttc ggcatgatcg ctgaacatat cacccggaac
    23761 cgcatctaat tgatgaaatc gaaaccaaac actgagtccg aaaaccaaaa tacacagtaa
    23821 attccagggg gataattgaa tctgaatcgg attgaatcga ctcccgatct tgattaactg
    23881 ttttttgatc gtttctttgg aaggaatccc ttttaatgag aaaacagcga aaagaacgga
    23941 aaaaaaccaa aaagtcaaat taataaaatt gaatcgatta ctgccgaata agataaatgc
    24001 aatgaaagcg cagccgaaag ataaagctgc ccaatatcga taaaatgttt cattaaataa
    24061 cgatgcggtt aatatttctt tttcacgaag gagaaggttt tcccggtcgc ttttttcttc
    24121 ctccatggat tttttttgaa caaaaaacga aataaacaaa ataagcgcag agaacccgta
    24181 taaaataacg gaaagcaaat aattccgatt gccatgaacg atcatcggct caagaaagag
    24241 ttgcgcaacg agagcaagca aggtcgcggc catcaaggcg gcgaacgata aattgaccct
    24301 ataaaatgga tgagaaacag tctcacgttg tattgaatcg gcgaaaatcg aaatcaagtt
    24361 tgttcgattt cgtaaaggct tttcatcaaa ctccgtttcg gttgtttgaa actcggattc
    24421 ttccggcaag atcgggcgat gccaattctg cggtttgagt ttcagtttca aataatccaa
    24481 aacgctcatt tcatccatag aaaattaact ccgtttttgg gcgatataaa aagtataagc
    24541 accgtccggt tgttcggcct cttcttcata ccatcttcga caaatctttt cggtcagcca
    24601 aagattccac tttgtcggaa ataaaatcca tttcccggta aggaaatggc aaatcaagga
    24661 gggtaatccg aaataatgcc cccattccaa cgtatgaaat gctccaggtg aaaaataatg
    24721 ccaatatcga atcaattcaa atccggcttg atccaagcgt tcttgccaga cctcaggcga
    24781 atcacaatga tgatgacggg atattttatt gaaaaaagaa cggtacgcat tcgcgagaaa
    24841 gtgaatatgg atgcgatcaa aaaaattgga aacagataaa tttgataaaa attgatgatt
    24901 cggaacacaa aaaataaatg taccgccggg tttgatgacc cggccggctt cgatcaccac
    24961 ggcatctact tccggtatgt gttccaatac ggaattactg ataattgtct gaaaataacg
    25021 gtcgggaaac gggatatcgg caccttcgca acggatgaca tttcgatatg cgccggtttt
    25081 cgccgcttca tataagggtc ctttccaagg atcgattccg acatcaatct gaaaatcaaa
    25141 cgtcctggaa gcaaaatgac cgtccccgca ccccaaatcc agaatcggcg cttttacctc
    25201 taaatcctga tagaatttcg cttcgacgga acgtaacagt ccgcgaaagt atggaagttc
    25261 gcgaatttga atccataaat aatctttttg atcggttaat gccttttctt tattcgattc
    25321 catattgtct gaaaagatcc tcataagctg cggctatttt gtccggttca taatactttc
    25381 gaatttcttc tgttttatcg gaataatctt ttttctcttg taaaacttcg acaatttttt
    25441 gagccagatc ttccgcattg ccgatttcgg caatttttcc cattccgtgt cgagtgaccg
    25501 gaacacgaat accgggaaga ttggaggcaa caaccggttt tccgttcatc atcgcttcaa
    25561 tctgtaccaa cccgaaagca tccgtacgat tcagactggg cattgccaat aaatcgatga
    25621 tttgataaaa ctttgccatt tctttcggcg gcaaacttcc caaaaatttc caatgccctt
    25681 cggcaatcat cgcatccaat cgcggtttca aacgctcata ataagcgcgt tcccccaaaa
    25741 cattatcata aggacccgca aaaaatacgc aggcattcgg atatgtattc aatattttgg
    25801 gtaatgcatc caacagaatt tcaacgcctt tatccgcggc aaaccggcat gccataccga
    25861 tcagcggtga tttatcttcc ggattccgtt tcatccgaaa ttctttgact tccgcttcgt
    25921 tcacttgagg caattcaacc ggcggcggaa taattctgac tttttccata tacttaggca
    25981 gaaaaaaaga ataatttgca taatcttcgg tatatgcgac gatggaatca caattcttac
    26041 cggcgatatc attcatccgt aataccgctt gattcgaaat ctgattgatc aatccggccg
    26101 gcatttgcag atcgcaatga taggtcaaaa cagaaaaagt tttctgacgc tttgcctgcc
    26161 gagctaccca gccggcatcg aattgcggaa gatgaatatt gactacatcc gattctttcg
    26221 ccagttttcc ggcttgccgc caaaaagcag gcataatggc acctttgctg atacgcatga
    26281 atatgggagc gcgaattact ctcacgccct ctactatttc ttcctccgga agtttcgaat
    26341 catgcctgga ggttaaaacg gtcacttcat gcccgcgttt cgcccatgcc ctcgcgattc
    26401 gatcggcata tatggttaac ccgctgacat aaggggtgta ataagttaac gcaaccagaa
    26461 ttttcatgcg aaaaggttcc ggttttcctc tgcccaatga tacaaacgag ttattccttc
    26521 cttaacggag acttcgggtc gccaatttag caagcgttcc gctttccgga tatccgatac
    26581 ataaatcact tgatccccgg gtcgccaatt gctgtaattg accgggattt tctctccgca
    26641 aagctcttcc aaaatcggtc cgaattcttt ccatacggaa atcacattgt tcggccctcc
    26701 gccgatatta aatatctgtc cggcggttgt ttccggtgcg tcgatcgccg catggtatgc
    26761 tctgaccaag tcatcgataa acagcagatc gcgaacctgt ttcccgtttc cataaattgt
    26821 tatcggtttt ttcaggagaa ccgcgatgat aaaccaagaa acccatccct gatcttccat
    26881 accgaactgt ctcggaccgt aaatcgcact ttggcgaaaa acgacggtcg gaatatcgta
    26941 gatacgcgaa tagtctcgca catattgatc gccggtccct ttcgaacaac cgtaaggaga
    27001 atgaaaatcc aacggctgac tttcggcaat gcccatcgga aaatcttcat aaacataatc
    27061 ttcctcacgt tcgatgactc ttacgttttc catccctccg tagactttat tggtggaggt
    27121 atataaaacg accggctgcc tgcctgattt tcgcaccgct tccagcaaat taaaagtccc
    27181 cgccgcattt atttcaaaat cctcgcgcgg gttggtcacc gaggtggtaa cggctacctg
    27241 cgcggcaaaa tgagctacca catctgcatt tcctaccgct ttctccaacg catccgcatc
    27301 acggacatct ccctgaatca aacggaagga atccgcaccg aattgactgc gtaaccaggc
    27361 gatattatgc cgtgatccgc ttcgagataa attatcaaaa atggttactt cccgattctt
    27421 tcttaataag tctgccgcga aattggaacc gataaaaccg gctcctccgg ttataaaata
    27481 ttttttagtc attttttgca tcctcttcag aaaggttcac ttttcgtaaa cctgaaaata
    27541 cctgtgaaaa actttgcccc tgtaccgcaa agccgattaa accccagata accggggtga
    27601 tgatccccat tgaatgattg aacataccga aaccgagtgc ttcggattga ttgataccga
    27661 aaattcttaa agcggcgacg aaggcgcctt cgatgacacc caatcccgcg ggagcggagg
    27721 ggatagctcc tcccaatgcc gtaatacttt gaacaaataa agacgaccat acgggaagat
    27781 tcgggaaaaa ggcgtgcata cataccaaca tggtcgcaat ccagcaaacc caagtcatca
    27841 gaatccaaaa aagccagatg ataaatttct tcggctgtgc caacacactc aatccgttta
    27901 aaagcgactg caatttcgga tataaaaatc ggccgaattt tgattccgat ttgatgattt
    27961 ttcccaaccg tttgacaaag ttttcctgat tcttggcgat taaaaacaaa agaaccaaac
    28021 cgatcaaaaa tatcgaaatc aaaacataat ataaatttgg tgaaaaatcg gcaccggaaa
    28081 ccaacggtat ggtcgcaaga agcataatca aagtgatgat gacatcaaac aatctttcta
    28141 aaagaacggt tgaaaaaaca aaaaagggac tgcgatgaat aacccctccc atgatgacag
    28201 cccgaccgat ttcccctaat ctcaaaggca ataaattgag caaatagccc tctgtaaccg
    28261 tccaaaaaca ttgagcccag gttggaatgc cttccaaggt gacccgccaa gctaaggctc
    28321 ggaaaataaa agccacggat tgcaaggcga atacgatcat gattaatcgg acggaaacgt
    28381 ttctgaaaga agcgatcaaa tcaccgaaat caacgacaaa gaccaaaata aggattatta
    28441 caacactgat caggatgccc ggcagcatct gtttccaaat accggatttt ttcgtatcgg
    28501 gttgatcgct cattgacttt attcctcatc caactgtaat atcgccataa atgcttcctg
    28561 tgggatttca acgttcccca ccatcttcat ccgttttttc ccgcgcttct gtttttccaa
    28621 caattttctt tttcgggtaa tatcgccccc gtagcatttt gccaatacat cctttcgcag
    28681 cgctttgaca ttcgcccgcg aaataatcct gccggaagca cttgcctgaa tcgccacatc
    28741 aaacatttgc cgcggtatta atgactttaa tttcgtgacc agcaactgcc ctttgtggta
    28801 agcatcttct ttatggacga tcgtcgcaag cgcatccacc ggttctccgc ccaccaaaac
    28861 ttccactttg actaaatttc ccggtctgta ttcatacatt tgataatcca tcgaagcgta
    28921 accccgagtt cgggatttca gttcatcaaa aaaatttacg attaattcag ccaaagggat
    28981 ttcaaaattc atctgcacac gatcgggagc cggatattct tcactgacaa atatgccgcg
    29041 tctttttcga gccaaatcca taatcgtgcc gtaaaattcc accggagaaa agatctcaag
    29101 cttcatccac ggttcccgaa tttcttttat ggttgactca tccggtaatt gaacggggga
    29161 atcaatcgta ataaccgttc cgtttaataa ttccacctga tattccaccg aaggagcggt
    29221 gacgacaata tcaagatcgt actcccgttc caaccgttcc tgcacaattt ccatatgaaa
    29281 caagcccaaa aatccgcaac gaaagccgaa attgagagcc tgcgacgtct ccggctgaaa
    29341 atctaacgac gcatcattta gttgaagctt ttccagcgca tttcgtaaat cgtcataatc
    29401 ttctccctca acgggataaa ttccggcaaa gaccatcggc ttggcttttt tataaccggg
    29461 gagcatttcg gcggccggtt cgagtgcgga tgtaatagta tcacctactc tgcattcttt
    29521 tacggttttc aatcccgtag cgatatatcc cacttccccc gcagtcagct gctgtacggg
    29581 tttcatcgcc ggagaaaaaa tcccgatctc cacgggtttt aaatcggctt tggtcgccat
    29641 catccgtaag gtcatattgg gcgatataat cccttctctg acacgaacat aggcaatcac
    29701 acctttataa gaatcatatt cggaatcaaa aatcagagct tgagtgggct ttgagtcgtc
    29761 accttgaggc ggcggaatca ttttgacgat taattccaac acttggtcta cgttccagcc
    29821 ttctttcgct gaaatgcgcg ggatttcatc cggatcgaca cccaacagat cggatatctc
    29881 ttttgcggtc ttatcggggt cggcagaagg caggtcaatc ttattgatta ccgggataat
    29941 caccaagtcc gattcgagag ccatatacag atttgcaagt gtttgcgctt cgatcccctg
    30001 tgtcgcatcc acaatcagaa ttgcaccttc gcaagccgcc aatgcgcggc tgacttcata
    30061 actgaaatcc acgtgccccg gtgtatcgat cagattgagt tcatagtctc ggccgtcttt
    30121 ggcggtatag ttcatccgga cagcggaagc tttaatcgtc acgcctttct ctcgttccag
    30181 atccatggaa tccaagattt gatccatcat ttgtcgatcc ggtactgtat tggttaactg
    30241 caataatcga tcagccagag ttgattttcc gtgatccacg tgtgcgatga tgcagaaatt
    30301 tcggatatgt tctttcataa tcgggaatta agtatatcat cgaaaaaatt gttgaatgac
    30361 ggggtattca taaagtatcc tgtctccact gcttttatat cgcttaaaaa caaatgggaa
    30421 taacctgaaa atttgaattt acaacttatg aacgcagaat aaaaatagat cttgatcctt
    30481 tttatggatt ggatttacgg acgctctttt aatttgtgtc acattttttc aaatacttta
    30541 ttatgctaac gtggtagagt gataaactat cggaagtttt tgacaattct atttaggaga
    30601 agctttatga aaaagtcttt atcgttgtct gttgcaatct ttttatttgc aaccttggta
    30661 atcggaatcg tttcggttca ggctgatggg atcaaaacat tggtcgtcgg ttttgatcag
    30721 gattttccgc cttacggcta tatcggcgaa gacggaaact tcacgggata tgatcttgaa
    30781 ttagcgaaag ctgccgcgga catcatgggg cttgagattg tgtatcaacc gatttcatgg
    30841 gattccaaag atctggaact ggactccgga accatcgatt gtatttggaa tgggttcacc
    30901 ataaatgagc gcgaagataa atacacctgg accgaagcat ataaaggcgc cggacaagtc
    30961 tttttagttt cggcagattc ggacattaaa acttttgacg acctgaaaga taaagttgtc
    31021 gcagttcaaa cggattcggc cggactatat gccattcagg atgagaagaa taaagagctg
    31081 ttagactcat tcaaagaact tttagtcgtc cctcaatata atgaagcttt tatggacctt
    31141 gaagcaggtg ccgttgacgc cattattttg gatattgatg ttgcgaagtt tcaaattaaa
    31201 ggcaaagaat ccaaatatcg aatcttggat gaatatctcc aaaaagaaga atacggcgtc
    31261 ggattcaagc tcggaaatac cgaactcaga gacaaagtcc aagaagcact gaatgaaatg
    31321 gctaaaaacg gtacctttgc aaaattatcc gaaaagtatt tcggttatga ttcctgcatt
    31381 atggctgcct gcctggcaaa tgaaaaataa gaaattgaca attacatctc ttcctttatt
    31441 gagggcactt ttaatcggaa gtgccctcaa tatttggaat cgatatttcg gggagaattt
    31501 ttatgggttt ggggttaatg ctcaataagt tatccgaagg gatggcaact tcggtcctga
    31561 tatttgtcct gactttgttg ttcagccttc cgctcggcct tttagtggct ctcatgagga
    31621 tgagccgtca caaagtctta cagaatctga tgcgaattta catctccgtt atgcgtggaa
    31681 cgccattaat gttgcaactt ttggtcgttt atttcgggcc ttattatatt ttcgggatca
    31741 aactttctcc cgattaccgt ttttgggctg taattatcgc ttttgtcatc aactatgccg
    31801 cgtatttcgc ggaaatctat cgaggcggca tcgcatctgt gccgtccggc caatatgaag
    31861 ccgcgaaagt cttgggatat tcgtcttcac agactttctt aaaaatcatt tttcctcaga
    31921 tgatcaagcg aatcatccca ccgattacga acgaagtgat tacgttgatc aaggacacct
    31981 ctttagcatt tgcggtcgcg gtaacagaaa tgtttacagt cgcaaaagcg cttgcgtcag
    32041 ccgaaaaaac aatgaccgct tatgtgtttg cgggagtttt ttattacgtt tttaatctga
    32101 ttgttgcatt tgttatggaa aatattgaaa aaagactaaa ttactataga ttgtgagcga
    32161 ttgaatggaa atattcagta tcaatgatgt caggaaaagt ttttcgaccc aaaccgatgt
    32221 tttatgcgga atgagtttat ccgttcagaa aggagaagta gtcgccatca tcgggccttc
    32281 cggatcaggg aaatcaacgc tgttacgttg tgcaactttg ttggaaacaa tggattcggg
    32341 tgaactggta tacgaaggga taaaagccgc atggaacgga cccgccggaa atgtcgttta
    32401 tgctccggaa gaaaccctta aaaaaataaa gagtaaattc ggtttggttt ttcaaaactt
    32461 caatcttttc cctcattatt ctgtgctgaa aaatgtgaca gatgcaccga tttgtgtgca
    32521 gaagagacaa aaagaggaag tatttgaaga ggcattgaca ttattaaaga agatggggct
    32581 gtcggataag gcagatgctt accctttcca attatccggc ggtcaacaac aacgggtttc
    32641 aatcgcaagg gctttggcga tgaatcccga tattcttttt tttgatgaac cgacctctgc
    32701 actggatccc gaattgacgg gtgaaatttt gcatgtcatt cgagacctcg ccgaagaaaa
    32761 aatgacaatg gtgatcgtaa cccatgaaat cgaatttgcg cggatcgttg ccaatcgggt
    32821 tgtttttatg gacgccggtg ttatcattga agaaggaaat tcagataaaa ttataagaaa
    32881 tcctgaaaac gaacggacga aagcttttct tcgcaaatta aatctttaac aaacagcgag
    32941 gtttcattat gtacgaaagt aaatcgaaaa gccccgaaac ggatgagctt tgtagagcca
    33001 ttctttcatt gaaatcagtc gaagagtgtt atcgcttttt cgatgatatt tttactatcc
    33061 gagaaataaa agatatttct caaaggttac aggttgccaa actgctccga tcaggagcta
    33121 tcttcaatga aattgtaaaa aaaaccgggg catccacagc aaccattgcc cgggtaaacc
    33181 gctgcctgaa ttatggtgcg gacggatata aaatgattct gaatcgtttg gatgaagagg
    33241 agaaaaaaga aatcaaagaa taatttctcc tttgaaaaaa attccggcag ttatcgtata
    33301 gaagcctttt ttactcttta caggagagat tttttttgct ctctactatt aaccgttttc
    33361 caccgtattt tgaaccgctt ctttaatatt aaattcattg ggtacagttt gcagccaaac
    33421 taaaaattct gtatttcctt tcggaccttt taaaggagaa cggatcaatc catatattcc
    33481 gtatcccatt tcttccgctt ttccgagcgt ttccattaaa acttttttat gaatttccgg
    33541 gtcacgaatc acaccggcac cctttgcggc ttcctttttc ccggcttcaa attgcggttt
    33601 gatcagactg attaccgttc cgccggagtc gccaaaccaa ttccggataa cgggcaaaag
    33661 aatttgtaaa gaaataaagg acgcatcaac ggttaccagt tcaacgggtt ccggtaagct
    33721 cttcaaatca cgggcattcg tcttttccat ggagacaact ctcggatcat ttcggatctt
    33781 ccaatgcaag attccgtatc cgacatcaat cgcatagact ttctttgccc catattgcaa
    33841 gagacaatcc gtaaatccgc cggtggatgc tccgacatcg gcgcaaattt ttccgttggg
    33901 gagatctgca ccgaaagctt ctaaggcacc tgataatttc tctcccccac gtgaaacaaa
    33961 acgcggtccg gcgtcaagcg agatccgatc atcggccgaa cagagagttg agggtttgat
    34021 aaccatatta tcattcactc gaacttttcc ggccatgatc aatttttggg ctaaagaccg
    34081 gctttccgcc aagccgttct cctgaaccaa aacatccaac ctcttctttt cagctttcac
    34141 ggggagactc cattgatttt ataagccata aagtgataaa gctcaataaa caaatttcag
    34201 aaacgatcat tttttggcgc gatttttaat agaatatgtt ttacaccgaa attattcgct
    34261 cggctatatt tcaatttcac aaaatgtcat cgttcatgtc tcaccgatta taatatacat
    34321 gctatggatc gaataaagcc ttatttgaaa gctatgcgta tcaatcaatg gaccaagaat
    34381 gcatttattt ttgctccgtt gatttttgac gggaaattaa cccattttcc ttcatttctg
    34441 cgaattctga tcggaacgat cattttcagc gttctctaca gcggaattta tgttttcaac
    34501 gatatagtcg atattgaagc ggaccgtctt catccgcgta aaaagaatcg cccgatcgca
    34561 tccggaacaa tatcaattaa gaatgctcgg atcttttcaa tcgctttgat agcatcggca
    34621 cttttatgcg gcggtttcct ccaatcggaa ttccttttga tcttagttct ggtcctcttc
    34681 attaatatca tctatacttt cttcttgaaa cgaatcccga ttatcgatgt gattgccatc
    34741 ggagtcttat ttcttttgag actggtcgcg ggcgttgtat taattacggt gaagatgttt
    34801 tctccttggt tatacttggt cacttttatg ctttctctgt atttgggctt cgggaaacgt
    34861 cgttccgaat tggcggcact taccggtaat tcttcacaaa ctcgtccggt cttaaacggt
    34921 tatagcctcg aattactgga tcaattaata acaattgttt caagcgtcac gattatggca
    34981 tattcgttat atacattttc cggacctact ttgcccggaa ataattttat gatgatcacg
    35041 atcccgattg tcatttatgg gatattcaga tatcactact taatccaaat caaacatgcg
    35101 ggcggagctc ccgaagaagt tctcttaaga gatcgacctt tacaagcgac aattttgatt
    35161 tatctcttag ctgtttgcat cggactttat ctttattaat aggttaatat gagtgaggca
    35221 ccggctaaag taatcttatt cggtgaacat gccgttgttt acggggaacc gggtattgct
    35281 attcctattt cagcgcttaa aacgagcgct tttttcgaaa acaatatcca taatgctttt
    35341 tcgattcaat ccgcaccgct cgggatggat ttgaattatc ttgaacttgc agacgatcat
    35401 ccgttgaaaa aattgatcat tctgctgatg aatgagtttg gaattacctc gttaccggaa
    35461 aagaaattac ggatcgaatc ggctattccg attgcatccg gtttgggatc gggtgcagct
    35521 tgttccgttg ccgtaattcg agcctttgcc gaggaatacc aaaaatcgat aagcacccaa
    35581 cggatttcgg agattgcttt tgaaattgaa aaaatctatc acggaacccc gtcgggatta
    35641 gataatacgg tgatcagctt cggagtaccg atttatttta taaaaaacca accgtttcaa
    35701 ttcatccaaa tcaagaaacc gctaaacctc ttgattgtag atacggaaat tgaaagcaac
    35761 acggcggaag ttgtgaaaga tattcgggat cattacgcga ccttttcgcc tctgattcat
    35821 gaaatcggag acatttctcg aacggcaaaa acagctttgg aggatggtga tatcaagctg
    35881 atcggtgaat taatgaataa gaaccatgat ttgctgaagc gattaaccgt ttcgtcaccg
    35941 gagcttgatt acctatacga aattgctata gagcaagggg caatcggcgc caaacttacc
    36001 ggttccggac gaggcggcaa catcatcgca ttggttgaag aagaagatca gctcgcatat
    36061 cttcgacaag ctttgcaaaa caagggggca aaggtcttaa attgatcaca ttattgaaat
    36121 taggcggttc gttaattaca aataaagaga aaccccattc cgtccgtaca gagaccattc
    36181 gaagagtcgg attcgagatt aaagaagctt tagattcgga tccggatatt aaactgattt
    36241 taggacacgg atcgggttca ttcggtcatg tcccggcttc tcgttaccat accctcgacg
    36301 gggttcagac ctcgagtgaa tggcgtggct ttgcagaagt ccatgccgaa gcagatgagt
    36361 tgaacaagat tacggtatcg atcctcagag gctgtggatt gccgattctc ggcttttcgc
    36421 cgttgagctc tgttttcacc cggaacagaa aggtaacccg atggaatatc gatcctattt
    36481 ttcaatccat tttgcatggc ttaattcccc tcatttacgg ggataccgtt cttgattccg
    36541 cactcggcgg taccattctt tccaccgaag agctttttgc ggcgctggcg atcaaatcgc
    36601 cgaaaccggt acggatcttg ttggcgggga tcgaagaagg catatggaaa gattatcaaa
    36661 agaaagattc tctaatcaaa gaaatggatc tttccgatcc cgagaatacg gaaagagact
    36721 ttattaaaca atccgcttat accgatgtga cgggtggcat gttctccaaa gttcaatcga
    36781 tggaagaatt aatcagaaag aatttaattc aggaagcttt aatattttcc ggagaaaaac
    36841 aaggaaatat caaagcggct cttttaggag gaacgcccgg aacaaaaatc tttcggtcgg
    36901 tttaatcggc accttctatc tgttcttttg cggaatgatg attcgacgaa tttatacctt
    36961 tcattccggc cggagtgaga agttttcggt ttatccttta aatcgtggca aaattaagaa
    37021 gcattaagtg ataataaatg tgaatccggt ataggatgaa caacattcat gtatgatcag
    37081 agttcatcgc tttttggtat attgacgata agatgaccgg aaccgacgat gaatcgataa
    37141 agggtttaag aaaatgaact atgcatctgc tttaaatact tttagaaaag aagaggttgc
    37201 aatcgcgccc tacgggatcc gttcaaaaga ttcaaaagga cgacaattcc ccgaagaaga
    37261 tcatgccttt cgctcttgct tccaacggga caaagatcga atcatccata ctacttcgtt
    37321 cagacgattg gaatataaga cgcaggtatt tataaatgat gaaggcgatt attatcgtac
    37381 tcgtctgact ca
//
LOCUS       DAOSMM010000017        37104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1158_length_37104_cov_17.029636, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000017 DAOSMM010000000
VERSION     DAOSMM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 37104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37104
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1158_length_37104_cov_17.029636"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..4005)
                     /gene="nifJ"
                     /locus_tag="PK898_04255"
     CDS             complement(415..4005)
                     /gene="nifJ"
                     /locus_tag="PK898_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU19278.1"
                     /translation="MARKNITIDANEAVAYVAYRLSEVIAIYPITPSSPIGEHADEWA
                     AKGVKNLWNTVPSIMEMQSEGGAAGAVHGALTTGSLTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVTARAVASHALSIFGDHSDVMATRQTGFALLASNSVQEAQDMALIAHAAT
                     LKARVPFLHFFDGFRTSHEISKIEQLDEETMKAMIDDELIIAHRNRGLNPEHPFIRGT
                     AQNPDVFFQGREAGNKYYEACPGIVQETMDQFAKLTGRSYHLFDYYGAKDAETVIIAM
                     GSGVETATETAKYLNSKGEKLGVLAVHLYRPFDAATMLKSLPASVKKICVLDRTKEPG
                     SAGEPLYLDVVTAVSEGFTNGTLPFEKMPIIVGGRYGLGSKDFTPAMVKGIFDELSKD
                     KPMNHFSVGIEDDVSGRSIAYDKNFLLPEAKTVRCMFFGLGSDGTVGANKNSIKIIGE
                     ETNNYAQGYFEYDSKKSGSTTTSHLRFGPDPIHAPYLIGENDANFLALHMFTFLEKLD
                     ITRFAAKGGTFLLNSFYGPDEVWDHLPAEVQKDIIDKELKFYVIDAFSVANKTGMGGR
                     INTIMQTCFFAISGVLPRDEAIEEIKKSITKTYGKKGDKVVAQNFEAVDSTLANLFEV
                     KYPKQVTSKIHRLPAVPPEAPEFVQKTTAPMIAAQGDKLPVSALPEDGTYPSGTSQWE
                     KRNIALVSPVWDPATCIQCGKCSMVCPHAVIRQKVYDASYLKNAPETFKSVDAKGFKE
                     FPGAKFTLQVSVEDCTGCGLCVYTCPAKNKENPQLKAINMAEQLPQRATESKNWAFFL
                     TIPDPDRTKIVPNSVKLAQQLRPLFEFSGACAGCGETPYIKLVSQLFGDRAIIANATG
                     CSSIYGGNLPTTPYTFNNEGHGPAWSNSLFEDNAEFGLGMRLTVDKQNQFAKELIADL
                     RGEIGDQLADGLLNADQSTEQGINEQRKRVKDLKDKLSKSKDPKAANLISIADALIKK
                     SVWIIGGDGWAYDIGYGGLDHVLASGKNVNILVLDTEVYSNTGGQASKSTPMAAVAKF
                     ASKGKDLPKKDLAYIAMTYGYIYVAKVALGANDNQVLKAMLEAEAYDGPSLIIAYSHC
                     INHGINMSLGLEQQKKAVESGHWPLFRYNPTKIEQGENPLTIDSKAPSIPLSDYAYSE
                     NRYKMLLKSDEARAEMLMKNAEKESGRRWELYKQLAELNYKTGVAGPKNE"
     gene            complement(4212..4931)
                     /locus_tag="PK898_04260"
     CDS             complement(4212..4931)
                     /locus_tag="PK898_04260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU19279.1"
                     /translation="MAVIEQDLNDTQEFDDEDEIRRLILEAEENQVWLEESGDEIPVE
                     KYENRRFEQKQFMKTEIISSEKQYQGHVFTVSKLNVRLPNGKIRHYDLVEHVPAVTIV
                     PVTNDGKLIFVRQFRMGANQEILELPAGILNGENGDEQPLLAAQRECQEETGYESRTI
                     KRIGGFYMTPGYCTEFIHVYLAQDLVWNPLPQDEDEFLKTELIPIEDAYRMAENGELN
                     DVKTIASLLMAQKDIRRKNEK"
     gene            complement(4969..6168)
                     /locus_tag="PK898_04265"
     CDS             complement(4969..6168)
                     /locus_tag="PK898_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOU19280.1"
                     /translation="MLKSSSFEDLNEDQRSIEQTQAELENKKAIRKDAILSTIIDAFS
                     SLIRTRPTQAAASISYYALFSVFPLILFVVVILSYFIEFTVIQKEIILLLENIIPGSE
                     TYVIENLQNILNNRTSTSIAASITLLWSSSRAFNGIISNIHRAWPESKGRGYFVNRFF
                     AILGIIAIIVFLVALTVFSVAINFSELVPLFDFQINQFVQFLMNLVVGTVLPIVLVYF
                     SSYLLYYYVPTVDVDKKAAQIGALVTSLTLKIFTGIFGLYILSPLNRYDLVYGSVTTI
                     ILMLLYVYFCTFIILFSAHLVAAITHYKLKKGIPTSFQQKTVPLSVKPSGKKVPVTKK
                     KRKSLAEISDEAGDQFAKWVEAKIVSIAAKRPNLRSKINELNAADKTDQIQKAIKNFF
                     GTLFRWK"
     gene            complement(6285..6821)
                     /locus_tag="PK898_04270"
     CDS             complement(6285..6821)
                     /locus_tag="PK898_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19281.1"
                     /translation="MENEVYRFETKLPVSVYILSGEPLSSRWLDWQFYDTGPGVFMIP
                     QNYFMGIRVQGFDNRQLKLLIDDLEPVSNLRYLHLAENRSITDDGILQLARLPQLQFL
                     NLSSCDITDAGLAFLPSLPKVTFLDLSYCNRISRGAAKYVRKLPYLHFLDLQGVIKVN
                     NADIKKFERRGLEIHRGS"
     gene            7241..8638
                     /locus_tag="PK898_04275"
     CDS             7241..8638
                     /locus_tag="PK898_04275"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOU19282.1"
                     /translation="MKGSAINQKTIPHRIYLTEDQIPTQWYNIRADMKNKPAPILNPG
                     TFKPLSKEELTPIFAEKLVDQELDNETRYIDIPDGIREIYKIYRPSPLIRAYNLEKAL
                     DTPAKIYYKYEGNNTSGSHKLNSAIAQAYYAKEQGLKGMTTETGAGQWGTALAEACSL
                     FGIPLIVYMVKVSYDQKPFRRTVMQTFGAEVHPSPTTQTQIGKKILAENPETTGSLGC
                     AISEAVETAVTHEGYRYFLGSCLNYVILHQSIIGIESKIAMESIGEYPDIVIGCAGGG
                     SNFGGLISEFVHDKLTGKSNPRLIAIEPASCPSFTRGKFAYDFADTGKITPLCKMYTV
                     GSQYIPPSDHAGGLRFHGMSPILSQLFHDGIIEAQAVTQRKVFEAAVLFAKTETILPA
                     PESAHAILGAINEALKCKESGEEKTILFGLTGTGYFDMTAYEAYLNGSMNDSSISEEE
                     LQRSLAQLPVVPEEA"
     gene            complement(8724..10214)
                     /locus_tag="PK898_04280"
     CDS             complement(8724..10214)
                     /locus_tag="PK898_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19283.1"
                     /translation="MKDYMSLQNGSDIRGIALDGDNGEKANLTSEAVFDITKSFVFWL
                     SEKAEKPSKILKIGVGTDSRLSADIMRQAILSGIEAGGANGFDCGMTSTPAMFMSTVF
                     PESDFDGAIMATASHLPWNRNGMKFFTKGKGTESADVTAILTKAQETLPEVPSGTRTK
                     FDLMPLYNSHLQTVIRNGIEDQSEFPLAGYKIAIDAGNGSAGFFATEVLEPLGADISG
                     SQFLEPDGRFPNHVPNPELPEVMEAAKKMTLESHSDLGIVFDTDVDRAAFVDDKGTIL
                     SRNRLIGVLAEIVSKKYPGCTIVTDSVTSDHLTKFISKLGCSHHRFKRGYRNVINEAI
                     RLNREGIICPLAIETSGHCAFSDNYFLDDGAYLAVVMIAETVKCRREGRSLADLASEL
                     KDPLESVEYRIKILNPDPQKYCESVLSDLREYTARQPSWKIAPENYEGVRISIPVFKG
                     WFLLRMSLHEPLMPLMIESDLEGGVNSILNEIKPFLEMYPDLELPF"
     gene            complement(10452..11135)
                     /locus_tag="PK898_04285"
     CDS             complement(10452..11135)
                     /locus_tag="PK898_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOU19284.1"
                     /translation="MEYKIEGAPFPVLICTLSNGEVMVTESGAMCWMTPNITMEATSR
                     GGLGKALGRMLSHESLFLNTFTASGTGMVAFGSSFPGMILPFDIEPGKELIVQKSGFL
                     ASEVGVELSMFFQKKLSSGLFGGEGFIMQRLSGQGKAFLEIDGYCKTYELGPGEKMLI
                     DTGYLAAMEPTCAMDIKKVSGGLKTMAFGGEGLFNTEVTGPGKVYLQTSPIKTLAGAL
                     LPYIPSKSS"
     gene            complement(11325..12392)
                     /locus_tag="PK898_04290"
     CDS             complement(11325..12392)
                     /locus_tag="PK898_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19285.1"
                     /translation="MKKNKFFFVIFVLTFLLPKTPVLADMTVKIGDEHSYRTLNEALN
                     ALPKNAGIVTFQLMNDLDNSADAVISVPDDKGITELKILCGKSEGVVKAGNIFQLFAN
                     GIPFLLGSSVEMDNAWIFGGAQADSKRIRKVQSTKVTVNGSVAYVFGGGGAFNGGTST
                     VAGTAEVELGESGKIYWEIFGGGLAQGEGSHAEVADTKLNIYGETDYALAGGLAQDGG
                     TATVGKTSSLSIMAGGKVNIALFGGGSAVGKGSIYETRDSFVNIYGNVAWAFGGDFVY
                     QGGTSYLSGTANVFIDQNGKAKAVYGGSFATDPGSRAEVNQTKVEVRGIAETMTECGE
                     HSKDGISIVRSKIDCTVNSVP"
     gene            complement(12553..12966)
                     /locus_tag="PK898_04295"
     CDS             complement(12553..12966)
                     /locus_tag="PK898_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19286.1"
                     /translation="MKTKRNQLFVLFILVLVTVFAGSSQASAADSVYPPAHYLGTLRE
                     YMNDIQRLPDIINTAICDKNEEEYQEKYQMLVKRISDVEWLMDEMGLKNTMNLQIIPT
                     TFLKHKECGIGKVVTGYADTAREKLQLELDKSGLE"
     gene            complement(13011..14309)
                     /locus_tag="PK898_04300"
     CDS             complement(13011..14309)
                     /locus_tag="PK898_04300"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF002823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HOU19287.1"
                     /translation="MNIDELIIKYPEILSLQSYRPFLWTNPHQNYGENLRFTITDIED
                     AENRFQRFAPFITEAFPETVLNKGIIESPLVEIRAMKAAMAERIGCSFSDHDRLFLKC
                     DHALPISGSIKARGGIYEVLKYAETLAIQAGLLRVQDNYRCLTEEKFKRFFSDYSLAV
                     GSTGNLGLSVGIMGRKLGFNVEVHMSTDAKKWKKELLRSVGATVYEHSGDFTQAVAQG
                     REQAKSNEKMHFIDDEYSTDLFLGYAVAAFRLKKQIAEQNISVDAEHPLAVIIPCGVG
                     GAPSGITFGLKQIFGKNVRCFTAEPTHAPAMLLGLMTELWDQISVKDVGIDGITAADG
                     LAVGRPSKLACEVLDSQISGCFTVTDDDLFKYLALLANTEKIQVEPSASAGFGGFDLI
                     RNRFFTTFDRVNYIVWSTGGGMVPPAEMTEYIKRGNRSFR"
     gene            14521..15213
                     /locus_tag="PK898_04305"
     CDS             14521..15213
                     /locus_tag="PK898_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOU19288.1"
                     /translation="MKVKALKAAFPFTIPVMIGYLFLGIGFGILLTGKGYPWWLSPLM
                     ALFIYAGSMQFVAIDIFSKAFNPLNALILTLTVNARHLFYGLSMLTKFKATDQKKPYL
                     IFALTDETYSLLCSTEPPQGIDRNWFYFFIALLDQIYWVTGCTLGSVAGSLVKFNTTG
                     IDFVMTALFVVIMVEQWESSKNHLPVLIGIGVSAICLLLFGKDLFILPSMISILAIVT
                     IFRKSIERRNVI"
     gene            15210..15542
                     /locus_tag="PK898_04310"
     CDS             15210..15542
                     /locus_tag="PK898_04310"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOU19289.1"
                     /translation="MTIRQILITVSVIVFGTILTRSLPFLIFPDSKESPDYIKYLGQV
                     LPYAIIGMLVVYCLKNVNLLNFPFGIPEGIALIYVVLVHHRRHNLLLSIGGGTILYMV
                     MVQFLFPV"
     gene            15651..16781
                     /gene="dnaN"
                     /locus_tag="PK898_04315"
     CDS             15651..16781
                     /gene="dnaN"
                     /locus_tag="PK898_04315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU19290.1"
                     /translation="MKVSVSQTNLAQALSIVSHAVATKSTLAILVNILITTEDGQLRL
                     SATNLEIGINYRIPADIIEEGAVTVPAKLMIDLINTLPNDTVYLELDTKTQTLSIRCL
                     QVITEIKGIDANEFPPMPVFEEDNAMTFDGSELRKMIQQVAFAASTDDARPILQGIYL
                     TAAGKTMTMVATDGFRIAIRNSTLEDSFTQPLSAIIPARALVELVRITNDSDKQIMMT
                     ISAGRNQVIFHLPQLDLASQTISGNYVDYKAIVPKSFQTTTVVSTAAFQKTCSQAMII
                     ARDSKYLTRFNIMGQENGMGKIQVTAQSEESGFYENYIDANVDGPDIQIAFNVLFLKE
                     VLDVITTPNVSLRTNTSVSPGLIVPLDNENEFQYVIMPMHLG"
     gene            16899..19124
                     /locus_tag="PK898_04320"
     CDS             16899..19124
                     /locus_tag="PK898_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU19291.1"
                     /translation="MFNRLVQDGYTTFAEFEERVRDFALMSQRREGLQNPFGQEGPNI
                     WENRLQRVRAQLINVEFSQHYSINVFNSIVNDVIQNRVVEKQPLFMWHNLEFASEETI
                     FDQALAIERMPAAERKEYESKLTGAKVVLIRRLLSEQLPYVEIAKNYFSIQDLIEISK
                     HRIGKGCIGGKSAGMLLARRILQGAEDEEIRSSATAKESFFIGADEYYSFLSINDRLD
                     LLDFRYDNEDQYWSRYPEIRESFKNTNFSKEIIESLERVVREFQGKPFIVRSSSLLED
                     GFNYSLSGLYESHVIANQGSVSEGLNKLLDAIRDIYAGTFDPRVLTYRLRNGLIDYPE
                     SMGILIQVITGNKQGNYLFPDISGVGASKSPFIWQGNSKNTSTDEGYIRVVCGLGTRA
                     IKRSGYDFSQIVVLNDPDKIRTVFSIDDPIYSQEMIDVINLKNNQFESLDVTSIVNEK
                     YNLFPWMAQIYKDGSLQPVVRGMNPDRYVINFNELIKKSSFTKLLRDILSILEKAYRR
                     PVLIEFTILVNLSNGALPSFQVQIDKCRPVNISDRMKGQWTFEEIDPNSKFLETDLYV
                     QNGLLKNIDYVVWIDSHELAKFAGNNPHIAAGLIRNLNKAMENQRFILMSNDRFGTSD
                     MGGGLPVAYSDITNAQAIVEISDSQKGKNADPMVGSQFYQNLLEAGIFTISINLERKG
                     CFFDQAFLETQLNQLTSWIDQPAAVKAVQVLPVKGYRGFEKLNIAFDQNRGKTTAYFS
                     K"
     gene            complement(19116..20144)
                     /locus_tag="PK898_04325"
     CDS             complement(19116..20144)
                     /locus_tag="PK898_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOU19292.1"
                     /translation="MDQFLPTGKIKPELLKDIIQRAPVFDHRIVLGPGIGLDCGVIDG
                     GDHYLVMKTDPITFATEEIGWYAVQISANDIATTGAKPEWMMITALLPEKSTTEQLIL
                     EITDQIYQAAAEINISLVNAHTEITVDLNRPILVCSLIGEAEKDRLVTPKGVRPGNEL
                     LLTKGIPIEGTALLAKEFPNLLKEVLSEDELIEAKAFTHIPGIGVTKDAQIALQNGTV
                     TGMHDPTEGGLASALWEFSTASGYALEIDPERIPIPELSAKICKRFHLNPLNTIASGA
                     LLLSVEAQDSAKIIQALEKADIPCSKIGLVTDREEVSVSDIKNKNLLPWPEQDDITKV
                     FQHENRLF"
     gene            complement(20362..21186)
                     /locus_tag="PK898_04330"
     CDS             complement(20362..21186)
                     /locus_tag="PK898_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU19293.1"
                     /translation="MVILDNGYIPNTLDEALQIRSENKVIPYAGGTDLMIKAEDVPYL
                     FLNKLAELQQISVDGDMIRIGAGCTYTSLLDSPITPKVLKEAIRLIAAPAIRNLGTVG
                     GNICNASPKADSALIFYVSDSKLRLRSVKGERILPIEDFYVGRGKTVLQPDELLIEIL
                     MPKKYLDNYYYQKVGARQALAISRVAFVGLFGMEDGKITCCSTAYGSVSDTILRFRNL
                     EAMLLGKTIAEAKELKGSFLAEYDKAIVPIRGRVSADYRKRVCMNLLEDYLTQNGI"
     gene            complement(21180..21656)
                     /locus_tag="PK898_04335"
     CDS             complement(21180..21656)
                     /locus_tag="PK898_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU19294.1"
                     /translation="MSNGISFILNGEKVVSKGKSGDRLLDILRDEFNLTGTKCGCKEG
                     ECGACSVLIDGKLANSCMVALGSIAGSEVVTIEGYSKTKRFQALSDAYAEFSAVQCGF
                     CIPGMVLASEALLSKNPHPSESEIREGISGNLCRCTGYNAIVHAIQTASEKGAGLW"
     gene            complement(21653..23770)
                     /locus_tag="PK898_04340"
     CDS             complement(21653..23770)
                     /locus_tag="PK898_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOU19295.1"
                     /translation="MDSITNSIKKRDHDPKISGRAKYVDDLRFEGMLYGKILRSTIAC
                     GIIKGIHIPDLPSGYTIVDKNDVPGVNIVHVVDDDTPVFSDGTVNYVGEPILMVVGED
                     QKVIKQILSEIIVDYEEAEPVLDMMKSETVFYHYGYTKGNPEKAFREADKIIDETFHT
                     GYQEHVYMEVQGMIGFYENGKSIVRGSMQCPYYVHTAVKKVMGAQTDQEVEVIQEEMG
                     GAFGGKEDYPSILGSQVAVAAKKVGRPVKCVFEREEDIICTSKRHPATMRYRAAIKDN
                     RIAAIQADVVYNCGAYTTLSPVVLQRGLIGATGVYTFDNIRVEGKGVKTNTTPNGAFR
                     GFGGPQTFFAIEMFMNHLAKEVGSDPLPFKRSYLSKQGDTTATSGKYHFYIPMPEMLE
                     KILKASDYERKYESYKRQTGRYRKGIGLSMVYHGCGFTGSGERDMIKGVVKLHKNVNN
                     TVEILTAGTDMGQGLKTTFSKIVAKRLEIPIDQIIIRNPDTDRVPNSGPTAASRSLMV
                     VGRLLDKAALKLKECWVDEEEQEITERYVHPDFVIPWDLNTFQGDAYPDNAWSVNVVE
                     VEVDTLTAVSKVIGAWGVYDVGTPIDMAIIQGQMQGGFLQGIGYGSIELMDVNEKGII
                     RNNTLSDYIIPTAMDVPHLETDVVNYPYIEGPFGGKGAGELPAVGGAPAYVSAMESAL
                     GKNLYKTPFSQEDAMKVLEEIKK"
     gene            complement(24207..24848)
                     /gene="udk"
                     /locus_tag="PK898_04345"
     CDS             complement(24207..24848)
                     /gene="udk"
                     /locus_tag="PK898_04345"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOU19296.1"
                     /translation="MNSDDESNSFHSITIGIAGGTGSGKTTFSQELVSRLNTDKIVYI
                     SHDSYYKDLSFMPLEERKKMNFDHPDSLDTDLMIQHLITLKEGKSVDIPVYDFKTFTR
                     TDQVEHITPQPVILVEGILIFAIKELRDLMDIKIFVDTDADIRFMRRLKRDIEERGRT
                     MESVYDQYLNEVRPMHEAFVEPSKRYADIIVPRGGRNDAALDMLAAKIRDYLS"
     gene            complement(24845..25516)
                     /locus_tag="PK898_04350"
     CDS             complement(24845..25516)
                     /locus_tag="PK898_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU19297.1"
                     /translation="MLYLKENHQELDAVVFDLGNVLLDYDPPRFMKDIGVPDQYHELM
                     GNILFNNKNIWPKWDLGVLNREGIIQQAILVEPSLKDEIIKYMENWDNYFYAVPHNVA
                     AMYRIKETGTKVYVLSNFSQETFEKIREHNAFLFDFDGMVISYQHHLIKPDPRIYQLL
                     IETYNINPARAVFIDDLEENVKAGREAGFFGIHLPPCADVTPYFIFPEGQETVENSEF
                     RQESK"
     gene            complement(25571..25843)
                     /locus_tag="PK898_04355"
     CDS             complement(25571..25843)
                     /locus_tag="PK898_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19298.1"
                     /translation="MAEHNSKKTSTKIEKEASEKPDELSQPATGLSDFIGIVLIAAGF
                     FNVPSLLKKPETQSDWMMLVIVLVFLISGFILLFLPRFLQKKRNKN"
     gene            complement(25855..26487)
                     /locus_tag="PK898_04360"
     CDS             complement(25855..26487)
                     /locus_tag="PK898_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU19299.1"
                     /translation="MQEEKEIDRDNLEICPNCLEPNEKDLANCAYCGMPLRKQTETAG
                     EALSSESTEKPDSDNSDESAKPNPPKAPAEEKKPNRGMAYAMRGMGIYLIFYAVTEIP
                     RSLKVEDPKNRMLAIVSDIIYMVAGVMMAWPLFKDYQKKRKEKKEKESAELIDSQTPD
                     NIGEETVHSSEIIDGTFKDNLNNAEEQADDSKAHEYEKEAVSQRDDHPSD"
     gene            complement(26491..27627)
                     /locus_tag="PK898_04365"
     CDS             complement(26491..27627)
                     /locus_tag="PK898_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU19300.1"
                     /translation="MDRTAKPRFPAISAGFVCLDIFPGLAHIDGNEFDRNFIPGRAVE
                     IDATTFATGGSVSNTGMALVKLGIETRLIGRIGKDPYGEILQQIYRQRGKNLADDLHI
                     IPDEATGHTYVIDPNGQERRFLFHPGSNDHFGLEDVPEESLDQAGLFHFGYPSSIKRL
                     SAGDGNDLTAIYLHAKQKGLTTSMDTSMPDPTRFSGQADWKNIFRKVLPYTDLFLPSF
                     EEILFMLDRERFERLNRSGDVIARAEKPLIRDLADQLLEMGAGIVMLKLGYRGLYLRT
                     AKIDRLNRLGRAAPSNLAAWEDLMIYQPSYKVSVAGTTGAGDACIAGLLAAFLRDTSP
                     DDAVKAANGAGACCCEKPDAFSGIQSWEQMCDRIDNGWEIVQEI"
     gene            complement(27627..28301)
                     /locus_tag="PK898_04370"
     CDS             complement(27627..28301)
                     /locus_tag="PK898_04370"
                     /inference="COORDINATES: protein motif:HMM:NF019031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOU19301.1"
                     /translation="MKRSEINQIIRNADAYLKKMNFYLPEWAYWDWETWKKKDRSSLH
                     EILTKEMGWDITDFALDDFYHRGMLLFTIRNGSTPETGYDKPYCEKIMILEDGQKCLL
                     HFHWDKVEDIINRGGGTLKLQFYNATADEQLDQKNPVILYVDGIRTEIEPGGILSLVP
                     GQSVTLPQRNYHSFWGDGKVLVGEVSKVNDDHTDNRFFDPIPRFPKVIEDEPIFRPMI
                     GDYKNL"
     gene            28553..>28794
                     /locus_tag="PK898_04375"
     CDS             28553..>28794
                     /locus_tag="PK898_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU19302.1"
                     /translation="MAGTIKEIARIANVSRGTVDRALNDRPGVNKEVAQRIKEIARSM
                     DYKPNSVAKALASRNRQFVIGVLINSIGNPFFDDVI"
     assembly_gap    28795..28894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28895..29450
                     /locus_tag="PK898_04380"
     CDS             <28895..29450
                     /locus_tag="PK898_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU19303.1"
                     /translation="GVNYLAYVGCDYYKSGETLGGMIGYITGGKARLGVIACTNNLEG
                     HRMRVEGCQRVLEKEFPQSEIVEVIEAEDNDEIAYQKTLRMLEERKEINALCFNAGGT
                     RGGLQAVRDLGLEKKLDIFTFDLTPAVIEGIQNGIVKSTVCQQPFTQGYQSIKILFDA
                     VLNDVIPENDHLYTDLSVKIKYNL"
     gene            29588..30898
                     /gene="xylA"
                     /locus_tag="PK898_04385"
     CDS             29588..30898
                     /gene="xylA"
                     /locus_tag="PK898_04385"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF003998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOU19304.1"
                     /translation="MKEYYPGIPKVKYEGPQSDNPFAYKFYNPEEIVAGKKMKDQLRF
                     SAAYWHTICAAGADMFGVATADKTFGKTDPMELAEAKAHAFFEIIDKLGIDYFAFHDR
                     DIAPEGSNLAETNKNLDKIVDLFETLMKEYKKNLLWGTANMFNNPRYMHGAGTSCNPD
                     AFAYAAAQAKKAIEVTVRLNGSGYTFWGGREGYETLLNTNMGLELDNMARLLHLATDY
                     GRSIGFKGAFYIEPKPKEPTKHQYDFDASTVIGFLRKYGLNMKLNLEANHGTLAGHTF
                     QHELRVARTEGYFGSVDANIGDLLLGWDTDQFPNDVYDSTFAMYEILKAGGFTTGGLN
                     FDAKARRASFQPDDIFISHIMGMDTMALGLRMADKLIRDGRIDQFVEDRYAGYKSGIG
                     KKIVDGTVTIQELEKYALEMGDVKTNKSGRQEYLEGVVNQILFG"
     gene            30903..32426
                     /gene="xylB"
                     /locus_tag="PK898_04390"
     CDS             30903..32426
                     /gene="xylB"
                     /locus_tag="PK898_04390"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOU19305.1"
                     /translation="MNYLIGIDLGTSGTKTVLFDENGHVHGSETIEYPLYQEQNGWGE
                     QEPDDWWHAACNSIRSVIAQSKINPSDIKGLGISGQMHGLVMLDKDGKVLRRSILWLD
                     QRTAKECEEITAKVGAKRLIEITANPALTGFTASKILWVRNHQPEIYEKCAHILLPKD
                     YVRYKLTGEFATEVSDASGMQLLDVPKRKWSDEVLEKLDIDKSLLGKVYESQEITGLV
                     SARAAAETGLKAGTPVVGGAGDNAAAAVGTGVVRDGLAFTTIGSSGVVFAHSSGIKID
                     PKGRVHTFCCAVPGAWHVMGVHQAAGLSLRWLRDNVCAEEVAEAEKRGIDPYDVMSEK
                     ADKITPGSDRLIYLPYLNGERTPHLDPNCRGVFFGLSGIHKRAHLIRAVMEGVSYSLR
                     DSISILREMDIETSEMTATGGGGRSFVWRQMLADNYHCPVKTIDNKEGPALGVAILAG
                     VGVGLFSSVPEACDSLIHDKSVQEPIRANSDVYDKYYPLYQKLYTDLKDSFNELATL"
     gene            32556..33617
                     /gene="chvE"
                     /locus_tag="PK898_04395"
     CDS             32556..33617
                     /gene="chvE"
                     /locus_tag="PK898_04395"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU19306.1"
                     /translation="MKKFIFVLLALVVLAVSVPVFAEGELIGVAMPTNSLQRWNQDGN
                     NMKTQLEKAGYKVILEFANNDVALQISQLENMILNDVKLLVVASIDGGALGDVLKTAK
                     DNGIPVIAYDRLITGTEYVDYYTSFDNYRVGKFQGDYIVKALDLENAAGPFNIEFFGG
                     SPDDNNAHLFYQGAIDVLQPYLDSGKLVCPSGQIDFNTVAILGWGTDDAQARMDNLIN
                     SYYADGKTKLDVVLSPNDSLALGIANSLKAAGFTLDNWPIITGQDCDIPNTINLIQGY
                     QAMSVFKDTRTLAERTVKMASQILNGEEVEVNNTTDYDNGVKVVPSYLCDPVVVDKSN
                     YKEMLIDSGYYTEDQIAVK"
     gene            33836..35425
                     /gene="gguA"
                     /locus_tag="PK898_04400"
     CDS             33836..35425
                     /gene="gguA"
                     /locus_tag="PK898_04400"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU19307.1"
                     /translation="MEKILLEMQSITKEFPGVRALDNVTFQVREGEIHALVGENGAGK
                     STLMNVLSGWYPHGSYEGKIIYDGEECKFFNLKDSEKLGIVIIHQELGLIPYLSIAEN
                     MFLGNETAHRVGPFEVIDWNECHNKAIMMLNRVGLDENPETLVKDIGVGKQQMVEIAR
                     ALAKNVRLLIMDEPTASLNDADSQRLLQMVQGMKEQGITCIIISHKLNEVEEIADAIT
                     VIRDGATIETLYKGIDEITQSRIIKDMVGREMTDQYPKRVPKIGQIGFEVKDWNVYHP
                     LYEGRKVIDNVSFNVRKGEIVGFAGLMGSGRTELAMSIFGKAYGKDISGKIIKDGKEV
                     EFKNIADAINQGVMYLTEDRKGAGLNLIDDIRHNITIANLQAISDRSVINENEEIKVA
                     NEYRKRLNIRSTGILQPTGTLSGGNQQKVMLSRGLFSDPDVLILDEPTRGIDVGSKYE
                     IYTFMNQLVEEGKCLIFISSEMPELLGMCDRVYVLNEGRIISELKGSEITQERIMDDI
                     LTDTRKRAKNGANSKSNENEN"
     gene            35388..36575
                     /gene="gguB"
                     /locus_tag="PK898_04405"
     CDS             35388..36575
                     /gene="gguB"
                     /locus_tag="PK898_04405"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU19308.1"
                     /translation="MEQTAKVTKTKINFRQYGMIIALVILALFFFVTTNGRFGKPMNV
                     YNIIMQNGYVLVLAIGMLLPILTGSIDLSVGSVVAVVSAFAGVMMYNWGMPVWITIII
                     CIISGIIIGLWQGFWIAMVNMPPFIATLGGMLIFRGLTLVILQGKTLAPLPSSYVQIS
                     SGYVKDYIGNLFGIKSSLNITTIVVAVIFCILILIGQLNSRKQKLKYGFEVSSVGSVF
                     LKTAVISAAILLVFWRLALYKGIPYVLLIVGVLALFYNFILTKTTLGRHIYAVGGNAK
                     AAELSGIDIRRVKYIIYINMAFMSAVAGIVFSARLNSASPLAGQGFEMDAIAACYLGG
                     ASASGGIGTITGALVGGLIMGILNNGMSLMGISSDVQQIVKGLVVIFAVAFDILSKNK
                     SSR"
     gene            36656..37102
                     /locus_tag="PK898_04410"
     CDS             36656..37102
                     /locus_tag="PK898_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU19309.1"
                     /translation="MDYKQLARQLVFLLQGETDQVAILANTAALLMTELENVSWAGFY
                     LEKEGELILGPFQGKPACIHIPLGKGVCGTAFVTQETLRVPDVHAFSGHIACDSESAS
                     EIVIPLSNRSGKLIGVMDLDSKKPDRFSEDDQAGLEMLAAALREFL"
BASE COUNT        10307 a         8206 c         7899 g        10592 t
ORIGIN      
        1 attgtaattt catatttttc agcatcaacc actttgagac tcccgggttc cgccgaaacg
       61 aatacaaaaa cagctaaaaa acagacaaga acaataaaaa aagaaagact gacatttttt
      121 ttcatagaat ttcctttcat acattttttc attatcaata cgcccactcc gttattttag
      181 ttccttatca cctgatgaac gagacaaatg gtcttaaaca aattggtgag agagcagagt
      241 tatttttaca tttttcccga tagtatcttg aagaatttca agcgattaat ctttatatta
      301 tttttcttct tcgattgata tcatccgaaa atcaatttag aaattttaac caaaagaagg
      361 ctgaaaaaca gcctcctttt gatgtattat ttattcaaaa aataagcaac ttccttattc
      421 gttcttgggt cccgcaacac cggttttgta attcaattcg gctaattgct tatacaattc
      481 ccagcgtctg ccggattctt tttcggcgtt cttcatcaac atttccgccc gagcctcatc
      541 actcttcagc aacatcttat aacgattttc gctataagca taatctgata gcggaatgga
      601 aggcgctttt gaatcaatcg tcagaggatt ctcaccttgc tctattttgg ttgggttata
      661 gcggaacaaa ggccaatggc cggattcgac agctttcttt tgttgttcca atcccagact
      721 catattaatt ccgtgattaa tacagtgtga ataagcgatg atcagagacg gtccgtcgta
      781 ggcttccgct tcaagcatgg ctttcaaaac ctgattgtca ttcgcaccca acgccacttt
      841 cgctacatag atataaccat aagtcatggc aatataagcc agatccttct tgggcagatc
      901 ttttcctttg gacgcaaatt tagcaaccgc cgccatcggg gtagacttgg aagcctgtcc
      961 gccggtattt gaatacactt cggtgtcgag tacaaggatg ttaacatttt tccccgatgc
     1021 caaaacatga tccagaccgc cataaccgat atcatatgcc cagccatcgc caccaatgat
     1081 ccagacactc tttttgatta aagcatcggc gattgaaatc aaatttgccg ctttaggatc
     1141 tttcgatttc gaaagcttat ccttcaaatc tttgacacgc tttcgctgtt cattaattcc
     1201 ttgttcggtg gattgatcgg cattgagtaa accgtcggcc agctgatcgc ctatttctcc
     1261 acgtaaatca gcaatcagtt cttttgcaaa ctgattctgt ttgtcaacgg tcaatcgcat
     1321 gcccagtccg aactcagcgt tatcttcaaa taggctgttc gaccatgccg gcccgtgtcc
     1381 ttcattgttg aaggtataag gagttgtcgg caagttaccg ccataaatgg aggaacaacc
     1441 ggtcgcattt gcgattatgg cacgatcgcc aaataattgc gagaccagtt taatgtatgg
     1501 ggtctcgccg catccggcgc aggcgccgga aaattcaaat aaggggcgca attgctgagc
     1561 caatttaacc gaattcggaa caatttttgt ccgatccgga tcgggaatgg tcaggaaaaa
     1621 tgcccaattc ttcgattcgg tggcacgttg tggtaactgt tcagccatat ttatggcttt
     1681 caactgcgga ttttccttat tttttgccgg gcaagtatag acacacaaac cgcagccggt
     1741 acaatcttcg accgagacct gaagggtgaa tttcgctccc ggaaattctt taaaaccctt
     1801 cgcatcgact gatttgaaag tttccggagc attcttcaga taagaagcat cataaacttt
     1861 ttgtcgaatg accgcatgcg gacagaccat ggaacatttt ccacattgga tacaggtcgc
     1921 cggatcccat accggagaaa ccaacgcaat attccgtttc tcccattggc ttgtccccga
     1981 cggataagtt ccgtcttcag ggagggcaga aaccggcaat ttgtcgcctt gggcggcaat
     2041 catcggtgct gtggtcttct gaacaaattc aggtgcttcc ggcggaacag ccggcagtct
     2101 atgaatttta ctggtgacct gcttgggata tttcacctca aataaattag caagggtaga
     2161 atcaaccgct tcaaagttct gagcgacgac tttatcacct ttcttcccat atgtttttgt
     2221 aatggacttt ttaatttcct cgattgcttc atcgcgcggc aagacaccgg aaattgcgaa
     2281 aaagcaagtc tgcataatgg tattaatcct tccacccatg cctgtcttat tcgcaaccga
     2341 gaaagcgtca atgacataga actttaattc tttgtcaata atatcttttt gtacttcggc
     2401 gggaagatga tcccaaactt cgtcaggacc gtaaaacgag ttcaatagga atgttccacc
     2461 cttcgcagcg aaacgggtta tgtccaactt ttcaagaaaa gtgaacatat gcaatgccaa
     2521 gaaatttgcg tcattctcac caattaaata aggagcatga atcgggtcag gaccaaagcg
     2581 caggtgactg gttgtagtag aaccggattt cttggaatca tattcgaaat atccttgtgc
     2641 ataattattc gtttcttcgc cgataatctt aatggagttt ttattcgctc cgacggtacc
     2701 atcggatcct aagccgaaga acatgcaccg gacagttttg gcttccggca gcaagaagtt
     2761 tttatcgtaa gctatgcttc tgccggaaac atcatcttcg atgccgacgg aaaaatgatt
     2821 catcggttta tctttggata attcatcgaa aatgccctta accattgcgg gagtaaaatc
     2881 tttggagccc aatccgtacc gaccgccgac aataatcggc atcttttcaa acggcaaagt
     2941 cccatttgta aagccttcgg atacagcggt tacgacatcc aaatagaggg gctcaccggc
     3001 agaacccggt tcttttgttc gatcaagaac acaaatcttt ttaacactcg ccggcagact
     3061 tttcagcatg gttgccgcat caaaaggtcg atacagatga acagcgagaa cacccagttt
     3121 ttcgcctttt gaattgagat attttgcggt ttcggtagcc gtttcaacgc cggaacccat
     3181 tgcaataatt actgtttcgg catcttttgc accgtaataa tcaaacaaat gataagaacg
     3241 accggtcagt ttcgcaaatt gatccatcgt ctcttggacg attcccggac atgcttcata
     3301 atacttattt cccgcttcgc gaccctggaa gaacacatcg ggattttgag ctgtgccgcg
     3361 aatgaaagga tgctccggat ttaaacctcg attacgatga gcgataatta attcgtcatc
     3421 aatcatcgct ttcatggttt cttcatcaag ttgttcgatc ttgctgattt cgtgagaagt
     3481 tcggaagcca tcaaagaaat gaaggaaagg aacacgtgct ttcaaagtcg ctgcatgagc
     3541 aatcaacgcc atatcttgcg cttcctgaac agaattagat gccagaagag caaagccggt
     3601 ttgtcgagtt gccattacgt cactgtgatc tccgaaaatg gatagagcat gactggcgac
     3661 cgcacgcgcc gtaacatgga aaacggttga agtcagttct ccggcgattt tatacatatt
     3721 cggaatcatc agcaacaaac cttgagacgc agtgaaagtc gtcgttaaag atccggtcgt
     3781 taaagcaccg tggacagccc cggctgcccc gccttcggac tgcatctcca tgatgctggg
     3841 gacagtgttc cagagattct tgactccttt cgctgcccat tcatcagcat gttcaccgat
     3901 cggtgaagat ggagttattg ggtagatggc aatgacttcg ctgagccgat aagccacata
     3961 tgcgaccgct tcattggcat ctattgttat gttttttcga gccatggatt attctcctaa
     4021 aaatataatt atttgtgttt gggtatgatt caaggttgac cctattctta atcaacagca
     4081 acatgtcctg cattcagaca accgaacaga atcggatgtc atttccggac cttctataat
     4141 tgtaccctct ttttaggggt taaatcattt ctgtttggga tcggtagtga aataaaataa
     4201 aaaagtgggc tttatttttc atttttccgg cgaatatctt tttgagccat taataaagat
     4261 gcaatcgtct tgacatcgtt caattcccca ttttcagcca tccgataggc atcctcaatc
     4321 ggaatcagct ctgtttttag aaattcgtct tcatcttgag gcagcggatt ccacaccaaa
     4381 tcttgagcaa gataaacatg aataaactct gtacaatagc cgggcgtcat ataaaaacca
     4441 ccgattcgtt ttatggtacg ggattcatat ccggtttctt cctggcattc ccgttgtgca
     4501 gccaaaagcg gctgctcgtc tccgttttct ccgttcaata ttccggccgg tagctcaaga
     4561 atttcttgat tcgcccccat tcgaaattgg cgcacaaaaa tcaatttccc gtcgttggtt
     4621 accggtacga tagtcaccgc agggacatgt tcaaccaaat cataatgtct tatttttccg
     4681 ttcggcaacc gtacatttaa ttttgaaact gtaaaaacat gtccttggta ttgtttttcg
     4741 gagctgataa tttcggtttt cataaattgt ttctgttcaa atctcctatt ttcatacttt
     4801 tcaacaggga tctcatctcc cgactcttcc aaccagactt gattttcttc cgcttctaaa
     4861 atcagacggc ggatttcatc ttcatcatca aattcttgag tatcattcaa atcttgttcg
     4921 attacggcca ttatgaatca attattcctt gatcaatgct aaaaaatttt atttccaccg
     4981 gaagagcgta ccaaaaaaat ttttaatggc cttttgtatt tgatcggtct tatctgctgc
     5041 gtttaattca tttattttgg accgtaaatt gggacgttta gcagcaattg atacgatttt
     5101 tgcttccacc cactttgcaa attggtctcc tgcttcatca gagatttctg cgagactctt
     5161 tcgtttcttt tttgttacag gaactttctt tccgctcggt tttacggaga gcggaactgt
     5221 cttctgttgg aaagaagtgg gaataccctt tttaagcttg taatgagtga tcgccgctac
     5281 taaatgagcg gaaaataaaa ttataaaggt acagaaatat acatacaaca acattaaaat
     5341 aatggttgta accgatccgt aaaccaaatc atatcgattg agaggactca gtatatataa
     5401 accgaaaatg ccggtaaaaa ttttaagggt taaagaggta accagggcgc cgatctgcgc
     5461 tgctttttta tcgacatcaa ccgtcggaac atagtaataa agcagataag aagaaaaata
     5521 aaccagcaca atcggcaaaa cagtaccgac aaccaaattc atcaggaatt gaacgaattg
     5581 attgatttga aaatcaaaca gaggaacaag ctcggaaaaa ttgatcgcaa ccgaaaaaac
     5641 tgtcagcgca accaagaaaa caatgatggc gattattcct aaaatcgcaa aaaaacggtt
     5701 gacaaaataa ccgcgtcctt tggattccgg ccatgctcga tgaatattac tgataatccc
     5761 attaaaagct ctggaactgg accataaaag tgtgattgaa gcggcaattg aagtggaagt
     5821 tctgttattg agaatattct gcaaattttc aataacgtag gtttcggatc ccggaattat
     5881 attttcaaga agcagaataa tttctttttg gataaccgta aattcaataa agtaagataa
     5941 aataaccaca acgaacagga ttaaaggaaa cacagaaaat aaagcatagt aggaaatact
     6001 ggctgccgct tgtgtcggtc tggttcggat aagtgaagag aatgcatcga tgattgttga
     6061 gagaatcgca tccttccgaa tggctttttt attttccaat tcggcttgcg tctgctcaat
     6121 cgatcgctga tcttcgttca aatcttcaaa agagcttgat ttcaacacgt ctatcccttg
     6181 catgacctac aatgtatatt actccttaaa tatcttgatt atcctgaaca tttcattaat
     6241 tcattccgaa ttaagtataa cctgcaaagc ttatgccaat ccggttaaga accgcgatga
     6301 atttccaacc cacggcgctc gaacttcttg atatcggcat tgttgacttt gatcacccct
     6361 tgtaaatcca gaaaatgaag gtagggtaat tttcgaacat atttcgctgc cccgcgcgat
     6421 attcgattgc aatagcttaa atccaagaaa gtaacttttg gcaaagaagg taaaaaagca
     6481 agtccggcat ctgtaatatc acaagagctc agatttaaaa attgcaattg cggtaacctc
     6541 gctaattgaa ggatcccgtc atcggtaata ctgcgattct cagccaaatg gagatatcgc
     6601 aaattggaaa caggttcaag atcatcgata agcagtttga gctggcggtt atcaaatccc
     6661 tgcacacgaa tccccataaa ataattttga ggaatcataa aaacacccgg acccgtatca
     6721 taaaattgcc aatccaacca acgagaggaa aggggttctc cggataaaat atagacagaa
     6781 accggtaatt ttgtttcgaa acgataaact tcattttcca taccatggat tataagttat
     6841 ttctggattt gtcatatttt taccgaattc ggtattgaca taacttcatc cggaatttaa
     6901 aatgcctgca atcaataaga aataaccgtt gattgagaat agtaggttca gcaacgcagc
     6961 atacagagag ttgcataaat ggtgagagag caacagccgg cagccgaact gaatggactc
     7021 aggagggcag gacgaaagca atttgcaagt agtgcctgac gggaactcca cccgttatca
     7081 agggagtacg catgatggtg cgtagaataa gcgggcgtga gaacacgtca agatgggtgg
     7141 taccgcagag gttgagtttt acgacctttg tcccaggttt aatcagaccg gggacaaagg
     7201 tctttttgtt tccccggtaa gaatcaaaag gagaaataaa atgaaaggct cagcaatcaa
     7261 tcaaaaaact attcctcacc gtatctacct taccgaagat caaattccga cgcaatggta
     7321 taacattcga gcagacatga aaaataaacc tgctccgatt ttaaacccgg gaacattcaa
     7381 accgctttcc aaagaagagt tgactccgat ttttgccgaa aaattggtgg atcaggaatt
     7441 agataacgaa acccgatata tcgatattcc ggacggaatt cgggaaatct acaaaatata
     7501 tcgcccgtcg ccgttaatcc gggcttacaa tttggaaaaa gctcttgata ctcccgccaa
     7561 aatttattac aaatatgagg ggaataatac atccggaagt cataagttaa attcggctat
     7621 cgctcaagca tattatgcga aagaacaggg attgaaaggg atgactacag aaaccggtgc
     7681 cggacaatgg ggaacggcat tggcggaagc ttgctcttta ttcggaatcc ctttgatcgt
     7741 atatatggtt aaagtcagtt atgatcaaaa accctttcgc cgcacggtta tgcaaacctt
     7801 tggtgccgaa gttcatccca gcccgacaac tcaaacgcaa atcggaaaga aaattttagc
     7861 tgaaaatccg gaaacgaccg gtagtttagg ttgcgcaatc agtgaagcgg tggaaacagc
     7921 tgtcacacat gaaggatatc gctattttct cggttcgtgt ctgaactatg tgatcttgca
     7981 tcaatcgatc atcgggattg aatcgaaaat tgcgatggaa tctatcggcg aatatcccga
     8041 cattgtcatc ggatgtgccg gcggaggttc caacttcggc ggattgattt cggaatttgt
     8101 ccatgataaa ttaaccggaa agtcaaatcc gcggttaatt gcgatcgaac cggcttcttg
     8161 tccctctttt acccgaggaa agtttgcata tgattttgcc gataccggta aaattactcc
     8221 gctgtgtaaa atgtataccg tcggcagcca atacattccg ccttctgatc atgccggcgg
     8281 actacgcttt catggaatgt cccccatcct ttcccagtta tttcatgacg gaattataga
     8341 agctcaagcg gtcacccaaa gaaaggtatt tgaagctgcg gttctttttg ccaaaacaga
     8401 gacaatcttg ccggcaccgg aatccgctca cgccattttg ggagcaatca atgaagcctt
     8461 gaaatgcaaa gaatccgggg aggaaaagac gattcttttc ggtttgaccg gaacaggcta
     8521 tttcgatatg acagcttatg aagcttatct caatggcagt atgaacgatt catcgattag
     8581 cgaggaagag ttgcaaagaa gtctggctca attgccggtt gtgccggaag aagcataaaa
     8641 tttagggcag gaaggcttta caatttattt gaaaatcttt ctgcccgatt cttttcatat
     8701 agatcaaacg accgttagac cgattaaaaa ggcaattcca agtcgggata catttctaag
     8761 aaaggcttta tttcgttcaa aatactgtta accccacctt cgagatcgct ctcgatcatc
     8821 agcggcatca gcggttcatg aagtgacatt cttaaaagaa accagccttt aaagaccgga
     8881 atagaaatcc tcaccccttc ataattctcc ggagcaattt tccatgaagg ttgccgggcg
     8941 gtatattccc gaagatcact caatacggat tcgcaatatt tttggggatc gggatttaga
     9001 attttgatcc gatattcaac gctctctaag ggatccttta attcggaagc caaatcggcc
     9061 aacgatcttc cttcccgccg acattttacc gtctcagcga tcatcacaac ggcaagatac
     9121 gcaccgtcat ccagaaaata attatcggaa aaagcacaat ggccggaagt ttcgatagcc
     9181 agcggacaga tgattccttc tctgtttaag cgaatggctt cattaattac atttcgatac
     9241 ccgcgtttaa agcgatgatg gctacatccc aatttggata taaactttgt caaatgatcc
     9301 gatgtgacgg aatcagtcac aattgtgcaa ccgggatatt ttttggaaac aatttccgcc
     9361 aaaaccccaa tcagacgatt acggctgagg attgttccct tatcatccac aaaagccgcc
     9421 cgatcgacat ccgtatcaaa aacgatcccg agatcactgt gactttcaag ggtcattttt
     9481 tttgccgctt ccatcacttc gggcaactcg ggattgggca catggttcgg gaaacggccg
     9541 tccggttcca aaaattgact cccggagata tccgctccca acggttccaa aacctcggtg
     9601 gcaaaaaagc ctgccgaacc gtttccggca tcgatcgcta ttttatatcc ggctaaagga
     9661 aattcacttt gatcctcaat cccatttctg ataaccgtct gtaaatgaga attgtaaaga
     9721 ggcatcaagt caaattttgt tcgtgttccg gaaggaactt ccggcaacgt ttcctgggct
     9781 ttggttaaaa tagcggtcac atccgccgat tccgttcctt tgcctttggt aaagaatttc
     9841 atcccgtttc gattccacgg cagatgactg gctgtagcca tgatggcacc atcaaaatcg
     9901 gattcaggaa agacggtcga cataaacata gccggcgtcg atgtcattcc gcaatcaaat
     9961 ccgtttgcac cgccggcttc tataccggat aaaatcgctt gccgcatgat atcggcagac
    10021 aatcgggaat cggtccccac tccgattttt aagattttgg aaggtttctc cgctttttcc
    10081 gaaagccaaa aaacgaaact tttcgtaatg tcaaaaaccg cttcagacgt aagattcgct
    10141 ttttctccgt tgtctccatc gagcgcaatc cctctgatgt cactgccgtt ttgaagggac
    10201 atgtaatctt tcatttcgaa acctcatttt aaatttgttg aatgaacctt tattatttta
    10261 taacatttgt catcaaagaa tcaatgccca tcggaaaatt cattcgaata tgtttttctt
    10321 tttggaatta tcactcatga taatttccgt acaaaaaaaa aggacgcccg attttgggca
    10381 tccatttagg aaatacaaaa tattcaattg tttggaaccg tctgaggaat cgattccgtt
    10441 aaatcaatca tctaactgct ctttgaagga atatagggaa ggagagcacc cgcaagcgtc
    10501 ttaatcggag atgtctgcaa gtatactttt cccggcccgg ttacttccgt attaaataat
    10561 ccttctccgc cgaaagccat ggttttcaat ccgccgctga cctttttaat atccattgca
    10621 caggttggtt ccattgccgc gagataaccg gtgtcaatca acattttttc tccggggccc
    10681 agctcatacg tcttgcaata tccgtcgatt tcaagaaaag cctttccttg accgctaagg
    10741 cgttgcataa taaaaccctc accgccgaaa agcccactgg ataatttctt ttgaaagaac
    10801 attgacaatt cgaccccgac ttccgatgcc aaaaagcctg atttttgaac aatcaattcc
    10861 ttcccgggtt cgatatcaaa cggaaggatc atgccgggga aggaagatcc gaatgcaacc
    10921 atgccggttc cgcttgcggt aaacgtattc agaaataatg attcgtggga taacattctg
    10981 cccaacgctt tgcccaaacc acctcttgag gtggcttcca tggtgatgtt cggagtcatc
    11041 cagcacatag caccgctttc agtcaccata acttcaccgt ttgacaaggt acagattaat
    11101 accgggaagg gagccccttc aattttatat tccatattac cctctcattt ctggattggt
    11161 tgttttgcca cttttcagta tatgccgaat tcttatggtg tcaaggatat ttttgggctg
    11221 cagcgttcca ctatgaataa gtcatataga aggcttggac gaccggaaaa ccggctgaga
    11281 tttggaaatc ccaattccga taaaaatctt tgcagattga aaaatcaggg aacggaattg
    11341 accgtacagt cgatttttga ccggacgatt gagattccgt ctttggaatg ttctccacat
    11401 tccgtcattg tttcggcaat acccctgact tcgacttttg tttgattcac ttcggctctg
    11461 ctgccgggat ctgtggcaaa acttcctccg taaaccgctt ttgctttccc gttttgatcg
    11521 atgaagacat tggctgttcc ggataaataa gatgtaccgc cctggtaaac gaaatctccg
    11581 ccaaaagccc aggcgacatt tccgtagata ttaacgaatg aatcccgggt ttcataaata
    11641 ctgccctttc cgacagcgct tcctccaccg aaaagcgcaa tatttacttt accaccggcc
    11701 ataattgaaa gagaagacgt ttttccgacg gttgcggttc ctccgtcttg agccaatcct
    11761 cccgccagtg cataatccgt ttctccgtag atgttcagct tcgtatcggc aacttcggcg
    11821 tgactgccct ccccttgtgc caatcctccc ccgaatattt cccaatagat ctttccgctt
    11881 tcgccgagtt ccacttctgc ggttcctgcc actgttgaag ttcctccgtt gaaagccccg
    11941 ccgccgccga atacataggc aaccgatcca ttgacggtta cttttgtgct ttgaactttt
    12001 cgaattcttt ttgaatcggc ttgagcgcct ccaaaaatcc aagcattatc catctccacg
    12061 gaagagccca atagaaacgg aattccgttg gcgaacagct ggaaaatatt cccggctttt
    12121 acaacacctt ccgattttcc gcacagaatc ttcaattcgg taatcccttt atcatccgga
    12181 acggagatta ccgcatcagc tgaattatcc aaatcattca tcaactgaaa ggtaacgatg
    12241 ccggcatttt tcggaagtgc attgagggct tcattcaacg tccgataaga atgctcatcg
    12301 ccgattttta cggtcatatc agccaaaacg ggtgttttgg gtaaaagaaa agtcaggacg
    12361 aagatcacaa aaaaaaactt attttttttc atgaggaaaa ttttatcaga atcggttttt
    12421 atcccgaagg aattgaatcc taaaaaatgt gaatagaatc ctttcgcgta cgaacctgct
    12481 tttatctcaa cattttgaaa aaaaccatcc cgacagcatc aggatggttt tttcaaaata
    12541 tgattcagga aattattcca gaccggattt atccaattcc agttgcagtt tttcacgggc
    12601 ggtatccgca taaccggtga caactttccc gattccgcat tctttatgtt tcagaaacgt
    12661 ggttgggata atctgcaaat tcattgtgtt cttcaagccc atttcatcca tcaaccattc
    12721 cacatcagaa attcttttta ccagcatttg atatttttct tgatattctt cttcattctt
    12781 atcgcaaatc gccgtattga tgatatccgg caatcgttga atatcattca tatactcacg
    12841 caatgtcccc agataatgag caggcgggta aacactatcc gctgcggatg cttgagagct
    12901 cccggcaaat accgtcacca gaaccaaaat aaataaaaca aacaattgat ttcgttttgt
    12961 cttcataaaa acatccctcc aaaaaattca tactttatca tatatacgcc ctatctgaaa
    13021 gatcggttcc ctcttttgat atattcggtc atttcggctg gcggaaccat tccgccgccc
    13081 gttgaccaga caatgtaatt cacccgatcg aaggttgtga aaaatcgatt acgaatcaga
    13141 tcgaatccgc caaaaccggc ggaagccgaa ggttcaacct gaattttttc ggtgtttgcc
    13201 aataaagcaa gatatttaaa taggtcatca tcggtaacgg tgaagcatcc gctgatctgt
    13261 gaatctaata cttcacaggc taatttggaa ggtcgcccca ccgcaagtcc gtccgcggcg
    13321 gtaatgccgt ctatcccgac atcttttact gaaatttgat cccacaattc cgtcatcaac
    13381 cccaacagca tcgccggagc gtgggtgggt tcggctgtaa aacagcggac attcttcccg
    13441 aagatctgct ttaaaccgaa agtgatcccg ctcggtgcac ctccgacgcc gcagggaata
    13501 atcacagcca gcggatgttc ggcatctacg gatatatttt gttcggctat ttgttttttc
    13561 agtctgaatg ccgccacggc atatccgaga aataaatccg ttgaatactc atcgtcgata
    13621 aaatgcattt tctcgtttga ttttgcctgc tcacgacctt gggctaccgc ctgagtgaaa
    13681 tcccccgaat gctcgtatac cgtcgctccg acagatcgga ggagttcctt cttccatttc
    13741 ttggcatcgg tcgacatatg aacctcgaca ttaaaaccga gttttcgtcc catgatcccg
    13801 acactcagcc cgagattgcc ggtagagccg actgccaggc tgtaatcgga gaagaatcgc
    13861 ttaaatttct cttcggtcaa gcagcgataa ttatcttgaa cccgaagtaa gccggcttga
    13921 atggcaagcg tttcggcata tttcaagact tcataaatcc cgcctctcgc tttgatcgag
    13981 ccggaaatcg gaagggcatg atcgcatttc agaaaaagtc tgtcatggtc tgaaaaagaa
    14041 caaccgattc tttcagccat tgcggctttc atggcacgaa tttcaaccaa gggagattca
    14101 ataatacctt tattcaaaac agtttcaggg aatgcttccg tgataaacgg agcgaatcgt
    14161 tggaatcgat tttctgcatc ttcgatatcc gttatcgtaa atcgcaaatt ttcaccgtaa
    14221 ttttgatggg gattagtcca gagaaacggc cgataggatt gaagggaaag aatttccgga
    14281 tatttgataa tcaattcatc gatattcatt tcactttctt tttatcatcc ctttcgtaat
    14341 tcgactttct cataattata ttgagttatc gttcgatatt tctgaacctt gttatcaata
    14401 ggaaagactc cttcctctgt catgattcag cgattatttc ttttcaaatc aggacacttc
    14461 caataaaatt tttttattcg gattgttctg atgaacctgt ctccaaagga aaaataacga
    14521 atgaaggtaa aagctttaaa ggcggctttc ccgtttacga tccccgtaat gatcggttat
    14581 ctatttctcg ggattggatt cggtatttta ttgaccggaa agggatatcc ctggtggtta
    14641 tcacctttaa tggccctttt tatttatgcc ggctccatgc aatttgtcgc gattgatatc
    14701 ttttcaaaag catttaaccc tttaaatgct ttgatattaa ctttgaccgt caatgccaga
    14761 cacctttttt atggtttgtc aatgttaaca aagtttaagg ctacggatca aaaaaagcct
    14821 tacttgattt ttgcattaac cgatgagact tattctctcc tgtgctcaac agagccgcca
    14881 cagggaatcg atcgaaattg gttttatttc tttatcgccc tgctggacca gatctactgg
    14941 gtcaccggtt gtactctcgg ttcggtcgcg ggatcactgg tgaaattcaa tacgacgggg
    15001 attgactttg taatgaccgc tttgtttgtt gtcattatgg tcgaacaatg ggaaagttca
    15061 aaaaatcatt tgcccgtttt aatcggaatc ggagtttcgg cgatctgttt gcttctgttc
    15121 ggaaaagatt tattcatcct tccctccatg atcagtattc ttgccattgt gacgattttc
    15181 aggaaatcga ttgagagaag gaatgtcata tgacgatccg acaaattctt attaccgtct
    15241 cggtgattgt tttcggcacc atcctcactc gatctcttcc tttccttatt tttccggatt
    15301 ctaaggaatc acccgactac attaaatatc tcggccaggt ccttccatat gcgattattg
    15361 gaatgcttgt agtctattgt ctaaagaatg taaatctatt gaattttccg tttggaattc
    15421 cggaaggaat tgctttgatt tacgtcgtgc ttgttcatca tcgccgacac aatttgttgt
    15481 tgagtatcgg gggaggtaca attttatata tggtaatggt acagtttctg tttccagtct
    15541 gatctctttc atgaaccgaa actgcggttc gttcgaatga gtcataaaaa atgaaaatag
    15601 tggaaagtgg tatactttta gcacaaaatt aaacacatcg ggagttggat atgaaagtct
    15661 ccgtatcaca aacgaatctc gcacaggctt taagtatcgt ttctcatgca gtcgcaacca
    15721 aaagtacact ggctatattg gtcaacattt tgatcacgac cgaagacggc cagctgagac
    15781 tttcagccac caatctggaa atcggtatca attatcgcat accggctgac atcatcgaag
    15841 aaggtgccgt gaccgttccg gcaaaactga tgatcgattt gatcaatacg cttccgaatg
    15901 ataccgtata tctggaattg gataccaaaa cccaaacttt atcgattcgt tgcctgcaag
    15961 tgattacgga aatcaaaggg attgatgcaa acgaattccc accgatgccg gtattcgaag
    16021 aagataacgc catgacattt gacggttccg aacttcgaaa aatgatacaa caagtcgcct
    16081 ttgcggcttc caccgatgac gcgcgcccga ttttgcaggg aatttacctg accgcagccg
    16141 gaaaaacaat gacaatggtt gcgactgacg gctttcgaat cgcaattcgc aactcgacgt
    16201 tagaggacag ttttacccag ccgttatcgg ccatcatccc cgcccgggct ttggttgagc
    16261 tggttcggat taccaacgat tcggataaac agattatgat gacgatctca gccgggcgga
    16321 atcaggttat attccatctg ccgcagcttg atttggcatc acagacgatt tccggaaatt
    16381 atgtggacta taaagccatt gttccgaaat cattccagac taccacggtc gtttcgacgg
    16441 ctgctttcca aaaaacctgc agtcaagcga tgattattgc ccgcgattcc aaatatctga
    16501 cacgttttaa cattatgggg caggaaaacg gaatgggaaa gattcaagtc accgcccaat
    16561 cggaagaatc cggtttttat gaaaattata ttgacgcgaa tgttgacgga cccgatattc
    16621 agattgcatt caacgtctta tttctgaaag aagtactgga tgtgatcacc acacccaacg
    16681 tttctctgcg caccaataca agtgtttcgc cggggttgat tgtccctctc gacaatgaga
    16741 atgaattcca atacgtgatt atgccgatgc atttgggata aaaatgacaa acagaaatga
    16801 taccgtcgac cagcaactca tttaattcga atgtattgtt ttcgattcaa attattctgg
    16861 cgcaatatcc gatattgcag gacagaatta gtgaagccat gtttaatcgg cttgtacagg
    16921 atggatatac tacttttgcc gagtttgagg agagggtccg tgattttgcc ttgatgtccc
    16981 aacggagaga gggactgcaa aatccgttcg ggcaggaagg accgaatatt tgggagaatc
    17041 gccttcaacg tgtccgcgct cagttgatta atgttgagtt ttctcagcat tactccataa
    17101 atgtattcaa ttccatcgtt aatgacgtca tccaaaatcg ggttgttgaa aaacaacctc
    17161 tttttatgtg gcacaacctc gaattcgctt cggaagaaac gattttcgat caggctttag
    17221 ccatcgagcg gatgccggca gccgagcgaa aagaatatga atcaaagttg acgggtgcca
    17281 aagtagtgct gattcgccgt cttttgagtg aacagttgcc ttatgtggaa atcgccaaaa
    17341 attatttttc cattcaggac ttaatcgaga tcagcaagca tcggatcgga aaaggatgta
    17401 tcggcggaaa gtcagccgga atgctgttag cccgtcgaat tttacaaggt gccgaggatg
    17461 aggaaattcg cagttccgct acggcaaaag agtcgttttt catcggtgcg gatgaatatt
    17521 attcttttct ttccatcaac gatcgattgg atttgcttga ttttcgttat gacaatgaag
    17581 accaatactg gagtcgttat ccggaaatac gcgagagctt taaaaatacc aatttttcaa
    17641 aagaaattat cgaaagtctc gaacgggttg tgcgggaatt tcaagggaaa ccgtttatcg
    17701 tccgatcttc cagccttttg gaagacgggt tcaattattc tttaagcgga ctctatgaaa
    17761 gccatgtgat cgccaatcag ggatcggttt ccgagggatt aaataaactg ttagatgcga
    17821 tccgcgatat ttatgccggt acttttgatc cgcgcgtgct gacttatcgt cttcgtaacg
    17881 gtttgattga ttaccccgaa agcatgggga tcctgattca ggtaattacc ggaaataagc
    17941 agggaaatta tcttttcccg gatatctcgg gagtgggcgc aagcaaaagt ccgtttattt
    18001 ggcagggaaa ttcaaagaat acatctacgg acgaaggtta tattcgagtg gtttgtgggt
    18061 tgggaacccg tgccattaag cgaagcggat atgatttttc gcaaatagtt gttctgaatg
    18121 atccggataa aatccgcacg gttttctcga tcgatgaccc gatttattct caggaaatga
    18181 tcgacgtcat caatttgaaa aacaatcaat ttgaatcgtt ggatgtaaca agtattgtca
    18241 acgagaaata taacctgttt ccttggatgg ctcagattta taaagacggt tccttgcagc
    18301 cggttgtccg aggaatgaat ccggaccgtt atgtcattaa tttcaacgaa ttgatcaaga
    18361 aaagttcatt taccaagctt ctgcgggata ttctttcaat ccttgaaaag gcgtacagac
    18421 ggcctgtcct tattgaattt acgattttgg tcaatctgtc gaacggggca ttgccctctt
    18481 tccaggtgca gatcgataaa tgccgcccgg tgaatatatc cgatcgaatg aaaggacagt
    18541 ggactttcga agaaatcgat ccgaattcaa aattccttga aaccgatttg tatgttcaaa
    18601 acgggttgtt gaagaatatt gattatgttg tttggattga ttctcatgaa ttggcaaaat
    18661 tcgccggaaa taacccgcat atagctgccg gcctcatcag aaacctgaat aaggcaatgg
    18721 aaaatcagcg atttattctg atgagcaatg atcgtttcgg cacttccgat atggggggtg
    18781 gtttgccggt cgcttatagc gatattacca atgctcaggc aattgtggaa atctccgatt
    18841 cgcaaaaagg caaaaacgcc gatccgatgg tcggctcaca attttatcaa aacttattgg
    18901 aagcgggcat ctttacgatc agtatcaatt tggaacggaa aggttgcttt tttgatcagg
    18961 cttttttgga gacacaactt aaccaattga cttcatggat tgatcaaccc gcagcggtaa
    19021 aagcggttca ggtcttgcct gtcaaaggat atcggggatt tgaaaaactc aatatcgctt
    19081 ttgatcaaaa tcgagggaaa acaaccgctt atttttcaaa ataaacggtt ttcatgctga
    19141 aaaactttcg taatatcatc ctgctccggc caagggagca ggtttttatt cttaatatcc
    19201 gagacggaaa cctcttcccg atctgtgacg agccctattt tcgagcaggg gatgtcggct
    19261 ttttccaacg cttgaataat ttttgcgctg tcctgcgctt ccaccgacag cagcaatgcg
    19321 ccggacgcga tggtattcaa cggatttaaa tgaaatcgct tgcatatttt tgcggataat
    19381 tccggaatgg gaattctttc gggatcgatt tccaatgcat aacccgaagc ggtcgagaat
    19441 tcccacaaag cggaagccaa accaccctcc gtcggatcgt gcataccggt aacggttccg
    19501 ttttgaagcg caatttgcgc atctttggtt actccgattc cgggaatatg ggtgaatgct
    19561 ttcgcctcga tcagttcgtc ttcactcaat acttctttca gaagattcgg aaattctttt
    19621 gccaataaag ccgtcccttc aatcgggatc ccttttgtta atagtaattc attccccggc
    19681 cgcacccctt tcggtgtaac caaacggtcc ttctccgcct cgccgatcag ggaacaaaca
    19741 agaatcggcc gatttaaatc aaccgtgatt tccgtatgag cattcactaa tgaaatattg
    19801 atttccgcgg cagcctgata gatttgatcg gtaatttcga gaataagctg ctcggttgtc
    19861 gacttctcgg gcaacaaggc tgttatcatc atccattcgg gtttcgcacc cgtcgttgcg
    19921 atatcatttg cgctgatttg aaccgcgtac caaccgattt cttcggttgc gaaagtgatc
    19981 ggatctgttt tcataaccag gtaatggtct ccaccatcga ttacgccaca atccaatccg
    20041 atcccgggtc cgagtacgat acgatggtca aaaaccggcg cccgttgaat aatatccttt
    20101 aataattccg gcttgatctt accggtcggc aaaaattgat ccaaaatcca gctccctaag
    20161 tttattcgca gaggcaagta taacattatg aaaacagaat ctctccggaa acgaataaaa
    20221 acctgaaggt caccgaattt cgattccttt tagcaaatcc cttaaaattt cgctgttcaa
    20281 cttgaaaata aaattttcaa ataaagaacc ggacttaatc gagtccggtt ctttattaac
    20341 cccgctgatc acttccaagc atcagattcc gttttgtgtc aaataatctt ccaataaatt
    20401 catacagaca cgcttccgat aatccgccga tacccgtccg cgaatgggaa caatcgcttt
    20461 atcatattcc gcaaggaatg aacctttcag ctcttttgct tccgcgatcg ttttccccag
    20521 cagcatggct tcaagattcc gaaatcgcag gatggtatcg ctgaccgacc cataagcggt
    20581 cgaacaacag gtaattttcc cgtcttccat cccgaataaa ccgacgaagg caactcgcga
    20641 gatggctaac gcctggcggg cgcccacttt ttgataatag taattatcca aatacttttt
    20701 cggcattaag atttcgatta ataattcatc gggttgcaag acggtttttc cgcgcccaac
    20761 ataaaaatct tcgatcggca aaattctttc gcctttgaca ctgcgaagac gtaattttga
    20821 atccgacaca taaaaaatca aggcgctgtc ggcttttggc gacgcattgc aaatatttcc
    20881 tccgaccgtc ccgagattgc ggatggccgg tgccgcgatc agacgaatcg cctctttcaa
    20941 aactttcggg gtgatcggac tgtccaatag agatgtatag gtacagccgg caccgattcg
    21001 aatcatgtcg ccatcgactg atatctgttg caactccgct aatttattca aaaaaaggta
    21061 aggaacgtcc tccgctttaa tcattaaatc cgttccgccg gcataaggga taactttatt
    21121 ttccgaacgt atttgtaagg cttcatccaa tgtatttggg atatatccgt tatcaagaat
    21181 caccataatc ctgctccctt ttccgatgct gtttgaatag catgaacaat cgcgttgtat
    21241 ccggtacagc ggcacaaatt gcctgagata ccttctctga tttcgctttc gctgggatgc
    21301 ggattctttg aaagcaatgc ttcggaagct aaaaccattc cggggatgca aaatccgcat
    21361 tgaacggcac tgaattccgc ataggcatcc gataatgcct gaaaacgctt tgttttgctg
    21421 tacccttcaa tggtgacaac ttcgctgccg gcaatgcttc caagcgccac catacaggaa
    21481 tttgcgagtt tcccgtcgat caacacggaa caagctccgc attccccctc tttacaaccg
    21541 catttggttc cggttaaatt gaattcatca cgaaggatat ccagcaatcg atcaccggat
    21601 tttcccttgg atacaacttt ttctccgttt aagataaatg aaatcccgtt gctcatttct
    21661 ttatttcctc caataccttc attgcgtctt cctgggagaa tggagtttta taaagattct
    21721 ttcccaacgc actctccatt gccgatacat aagccggcgc acctcctacc gcagggagct
    21781 cccccgctcc ttttccgccg aacggacctt cgatataagg atagttaacc acatccgttt
    21841 ccaaatgggg gacatccatt gccgtgggaa taatatagtc gctcaatgta ttattgcgga
    21901 taattccctt ttcattgaca tccatcaatt cgatggaacc atacccgatt ccctggagga
    21961 acccgccctg catctgtccc tggattattg ccatgtcaat aggcgttccg acatcataaa
    22021 cgccccaagc tccgattact ttactgaccg cggttaaagt gtcgacttca acttcaacaa
    22081 cgtttaccga ccaagcatta tccgggtaag catctccctg aaatgtattc aaatcccacg
    22141 gaatcacaaa atccggatgg acataacgtt cggttatttc ctgttcttct tcatcaaccc
    22201 aacattcctt taatttgagc gcggctttat ccaacagcct gccaactacc attaaagagc
    22261 gactggcggc ggtcggtccg gaattgggaa cccgatcggt atccgggttc cgaataatga
    22321 tctgatcaat cggaatttcg agcctttttg ccacgatctt gctgaaagtc gtcttcagcc
    22381 cctgtcccat atccgttccc gcggtcagga tttcaaccgt gttattgaca tttttatgaa
    22441 gttttacgac ccctttaatc atgtcacgtt cgccgcttcc cgtaaacccg cagccgtggt
    22501 acaccatgga caacccgatt cctttccgat atctgccggt ttgccgctta taactttcat
    22561 atttccgttc ataatcactg gctttcagaa ttttttccaa catctccggc atcggaatat
    22621 agaaatgata ttttccgctt gtagcggttg tatcaccttg tttggaaagg tacgatcgtt
    22681 tgaacggcaa cggatcgctt cctacttctt ttgcaagatg attcataaac atttcaattg
    22741 cgaaaaatgt ctggggaccg ccgaaaccgc gaaatgcgcc gtttggcgtg gtattggttt
    22801 tcacaccttt tccttcgact cgaatattat cgaaagtgta aactccggtc gcaccgatca
    22861 aaccacgttg caaaaccacc ggagagaggg ttgtataggc gccgcagtta taaacaacat
    22921 cggcttgtat tgccgcaatt cgattatcct tgattgccgc ccgataacgc atggtggcgg
    22981 gatggcgttt agaggtgcaa ataatatctt cttcccgttc gaaaacgcat tttaccggac
    23041 ggccgacttt tttcgccgct acagccactt gtgatcccaa aatggaagga taatcctctt
    23101 ttccgccgaa agctcccccc atttcttcct gtatgacttc aacttcctga tcggtttgtg
    23161 ctcccattac ttttttaacc gcagtatgga cataatacgg acattgcatc gaacctcgca
    23221 ctatgctttt tccgttttca taaaaaccga tcatcccctg gacttccata taaacatgtt
    23281 cctgataacc ggtatggaaa gtttcatcaa ttattttatc cgcttcccga aaggcttttt
    23341 ccggatttcc tttggtataa ccataatggt agaaaacagt ttcggatttc atcatatcca
    23401 aaaccggttc cgcttcttca taatcgacaa taatttcact caatatttgt ttgatcactt
    23461 tttgatcttc gccgacgacc atcaagatcg gctcacccac ataattgacc gtaccgtctg
    23521 aaaaaaccgg tgtatcatca tccaccacat ggacaatatt gacgcccggc acatcatttt
    23581 tatccacaat ggtataaccg gagggtaaat cgggaatgtg aattcctttg atgattccgc
    23641 aggctatggt tgatcgcaaa attttgccgt aaagcatgcc ttcaaatcgt aggtcatcca
    23701 catattttgc cctgccgctg attttcggat catgatctct ttttttgatc gaattagtaa
    23761 ttgaatccat tcttgctcct atattatccg ctgactcgaa agtcaatttc tatatctttt
    23821 tttacttttg cctttgatta tttctaaatc gggtcaatct gacatctttt tttgatttag
    23881 aagagttacc gttccttttt gaatgaattc agaaaggaat ttctaagcgt aggtcaaccc
    23941 catttttgtt caatcgactc tgtttctctc tgtcaaacta atcaaaatcg gctgaggtcg
    24001 ctccttgcaa tcttatttcg cctaatagaa ttattctaca tggcaaataa tttgtcatat
    24061 ggtattttac ctgtatttgt ctgacaaagg taataaaaat gtgtaaaaaa tcagggtttc
    24121 tcgaagccgt aatcgttaaa aagcatctcg aatgaatcta aaaaagcaga atgccgaacg
    24181 gtccttctcg catgtgaatg aatggctcag gaaagataat ctctgatttt agcagccagc
    24241 atatccagtg ccgcatcatt tctgccgccg cgtgggacga taatatcggc atatcgtttc
    24301 gaaggttcaa caaacgcttc atgcatcggg cgtacttcgt tgagatattg gtcataaacc
    24361 gattccatcg ttcttccccg ctcttcgata tcccgtttca accgtcgcat aaaacgaata
    24421 tccgcatccg tatcgacaaa aatcttgata tccatcagat cccggagttc tttaatcgca
    24481 aagatcaata ttccctcaac caaaataacc ggctgaggag taatatgttc cacttgatcg
    24541 gtacgtgtga atgttttaaa gtcatagacc ggaatatcca cactttttcc ttctttcagc
    24601 gtaattaaat gttgaatcat caggtctgta tctaacgaat cgggatgatc aaaattcatt
    24661 ttcttacgct cttccagagg catgaacgaa agatctttgt agtaggagtc gtgggaaata
    24721 taaacaattt tatcggtgtt cagtctggag accagttcct gtgaaaaagt ggttttcccc
    24781 gaaccggtcc caccggcaat tccgatcgtg attgagtgaa aagaatttga ttcgtcatcc
    24841 gaattcattt tgattcctgt ctgaactccg aattttccac cgtttcttgt ccttccggaa
    24901 atataaagta gggtgtgaca tcggcgcatg gcggcaaatg aataccgaaa aatcccgctt
    24961 ctcttccggc ttttacattt tcttccagat catcaatgaa gaccgcccga gccggattga
    25021 tgttatacgt ttcgattaat aattgataaa ttctcggatc cggtttaatc aaatgatgct
    25081 gataagagat caccataccg tcaaagtcga acagaaatgc attgtgttct ctgatcttct
    25141 caaaagtttc ctgagaaaaa ttggaaagaa catagacttt ggtgccggtc tctttaatcc
    25201 gatacatggc tgccacatta tgaggaacgg cataaaagta attgtcccaa ttttccatat
    25261 attttatgat ttcatctttg agagaaggtt cgaccagaat ggcctgttga ataattccct
    25321 ctctgttcag gacgcccaaa tcccacttcg gccatatatt tttattattg aaaaggatgt
    25381 ttcccatcag ttcatgatat tgatcgggaa cgccgatatc cttcataaat ctgggaggat
    25441 catagtccaa cagaacattc ccgagatcaa atacaacggc atccagttcc tgatgatttt
    25501 ctttcaaata caacatgtga tttttataac ctcatcttcg aattactttt caaacaatca
    25561 ttaccgcgtc ttaattttta ttcctcttct tttgcaaaaa acgaggcaaa aacaggagga
    25621 taaaacccga tattaagaat accagaacaa tgacaagcat catccaatcg ctttgggtct
    25681 caggtttttt caacaatgag ggaacattga aaaaaccggc ggcgattaag acaattccga
    25741 taaaatcaga aagtccggta gccggttgag agagctcatc cggcttttcg gaagcttcct
    25801 tttcaatctt tgtgctcgtc ttttttgaat tgtgttcagc cattttataa aaatttaatc
    25861 cgacggatga tcatctctct gagaaacagc ctctttttca tactcatgtg cctttgaatc
    25921 atccgcctgc tcttccgcat tgtttaagtt gtccttgaac gttccatcaa tgatttcgga
    25981 agaatgaacc gtttcttcgc ctatgttatc cggcgtttgc gaatcgatga gctctgccga
    26041 ttccttctct ttcttctctt ttcttttctt ctgataatct ttgaataaag gccatgccat
    26101 cataacgccc gcgaccatat aaataatgtc gctgacaatc gcaagcattc tgttcttagg
    26161 atcttccact tttaaagatc ggggaatttc ggtaaccgca taaaaaatca aataaatacc
    26221 catcccccgc atggcataag ccatcccccg gttgggtttt ttctcttccg ccggagcctt
    26281 gggaggattg ggttttgccg attcatccga attatccgaa tccggttttt cagttgattc
    26341 ggatgaaagg gcttcaccgg cggtttccgt ctgttttctt aatggcatac cgcaataagc
    26401 acaatttgcc agatcctttt cattcggttc cagacaattc ggacagattt cgagattatc
    26461 gcggtctatt tctttttctt cttgcatagg ttatatctcc tgaacaattt cccagccgtt
    26521 atcgatccga tcacacattt gttcccaact ttgtattccg ctgaacgcat ccggtttttc
    26581 gcaacaacat gcacctgctc cgtttgcggc ttttactgca tcgtcgggag acgtatcccg
    26641 cagaaaagcg gctaaaagac ccgcaatgca cgcatctccg gcaccggttg ttcccgcaac
    26701 ggacacttta taagacggtt gataaatcat caggtcttcc cacgcagcca aattggaggg
    26761 agctgcccgc cccaatcggt ttaatcggtc tatcttggct gtccgtaagt ataatccacg
    26821 atagcctaat ttgagcatga caattccggc ccccatctcc aacaattgat cggccaaatc
    26881 gcgaatcagc ggtttttccg ctctggcgat tacatcaccg ctacggttca gtcgttcgaa
    26941 cctttcacgg tcaagcataa acagaatttc ctcaaaactc ggtaagaaca agtccgtata
    27001 aggaaggact tttcgaaaaa tatttttcca atccgcttga ccggagaacc gcgtcggatc
    27061 aggcatggac gtatccatcg aagttgtaag tcccttttgt ttcgcatgca gataaattgc
    27121 cgtaagatca tttccgtcac cggcggataa tctcttgatg gatgacggat agccgaaatg
    27181 aaaaagaccg gcttgatcca agctttcttc gggaacatct tccaacccga aatgatcatt
    27241 ggaaccggga tggaaaagaa aacgcctttc ctgtccgttc ggatcgatta cgtaagtatg
    27301 tccggtcgct tcatcgggga taatgtgaag atcgtccgcc aaattttttc cgcgttgacg
    27361 ataaatttgt tgcagaattt ccccatacgg atcttttccg attcttccga ttaaccgggt
    27421 ctcgatacct aatttgacca gagccatgcc ggtattggaa acggaacccc cggtcgcgaa
    27481 tgtggtcgca tcgatttcta ccgcgcgacc gggtataaaa ttccggtcaa attcattccc
    27541 atcaatatga gctaagccag ggaatatatc cagacagaca aaccccgccg aaatagcggg
    27601 aaagcgtggt tttgcggttc tgtccattac agatttttat aatccccaat catcggacgg
    27661 aaaataggtt cgtcttcgat taccttcggg aaacggggaa tcggatcgaa gaaacgatta
    27721 tcggtatgat catcattcac tttggagact tctccgacca aaactttccc gtcgccccag
    27781 aaactgtgat aattacgctg cggtaatgtg acgctttggc ctgggacaag cgaaagaatt
    27841 cccccgggtt ctatttccgt acggatgccg tcaacatata aaattaccgg atttttctga
    27901 tccaactgtt catccgcagt tgcgttataa aactgaagtt ttaatgttcc gccgccgcgg
    27961 ttgataatat cctcaacttt atcccaatgg aaatgaagaa gacatttttg cccgtcttcg
    28021 agaatcataa ttttctcaca atacggttta tcgtaacctg tttccggcgt actgccgtta
    28081 cgaatggtaa aaagcagcat tccccgatga taaaagtcat ccaaagcgaa atccgtgata
    28141 tcccaaccca tctcttttgt gaggatttca tgcaaagatg agcgatcttt ctttttccat
    28201 gtttcccaat cccaataagc ccattcggga agataaaagt tcattttctt tagataggca
    28261 tccgcattac gaattatttg attaatttcc gagcgtttca taatattttc cccattaaaa
    28321 aataagattc cctaaattaa ttattttacc ttcagaatga tcgtttcccg aaagccggtg
    28381 aatcaattta acttatcgca aattatcgcg aaaatctttt tcgacttccg cttatgggta
    28441 gagattttgc tccggaagct taagaaaccc tattaaaatt taatgtttcg aatgtaaaat
    28501 attaaacaga actaagatta acgctgaatc gacatcatta aggaaatcaa agatggccgg
    28561 aacaataaag gaaatagcaa gaattgccaa tgtctcccgc gggacagtag atcgggctct
    28621 gaacgatcgt cccggggtca ataaagaagt tgcccagcga attaaagaga ttgcgcgatc
    28681 gatggactac aagccgaatt cggtcgcgaa ggcgcttgct tcacggaatc gtcagtttgt
    28741 aatcggtgtt ttgatcaaca gtatcgggaa cccctttttt gatgatgtca ttttnnnnnn
    28801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggggt aaattatctc gcttatgtgg
    28921 gatgtgatta ttataaaagc ggcgaaacat tgggagggat gatcggctat attacaggtg
    28981 gtaaggctcg attgggagta atcgcctgta ccaataactt ggaaggtcat cgcatgcggg
    29041 tggaaggatg tcaaagggtt ttagagaaag aatttccgca aagcgaaatt gttgaagtaa
    29101 ttgaggcgga agataacgat gaaattgctt atcaaaaaac ccttagaatg ttggaagaga
    29161 gaaaagaaat caatgccctt tgttttaatg ccggcggtac aagaggcgga cttcaggctg
    29221 tccgagattt agggcttgag aagaaattgg atattttcac ttttgatttg acaccggcag
    29281 tgatcgaagg cattcaaaat ggaatcgtta aatcaaccgt gtgtcaacaa ccgtttaccc
    29341 aaggatatca aagtatcaaa attctttttg atgccgtact gaacgacgtt attcccgaaa
    29401 atgatcatct ttataccgat ttaagtgtca agataaaata caacctttag ttttaaaata
    29461 atagtcattc atcacaaaaa aatagttaca ttagtaagta gcagttaccc gcgagattca
    29521 tagtataatc aaattattct tcgtgtacga gcacgatgaa taaagttcag actcaggagt
    29581 gtaatccatg aaagaatatt acccgggaat ccccaaggtt aaatatgaag ggccgcaaag
    29641 cgataaccct tttgcatata aattttataa tccggaagaa attgtagccg gcaaaaaaat
    29701 gaaggatcaa ttacgctttt cagccgctta ctggcatacc atttgtgcgg ccggagccga
    29761 tatgttcggg gtagccacag cggataagac attcggcaaa accgatccga tggagctcgc
    29821 tgaagccaaa gcgcatgctt tttttgaaat tatcgataag ctgggcatag actatttcgc
    29881 attccatgat cgtgatattg ctccggaagg ttcgaacctg gctgaaacca ataaaaattt
    29941 ggataaaatc gtcgatttat ttgagacact gatgaaagaa tataaaaaga atctgctttg
    30001 gggaaccgcc aacatgttca ataatccgcg ctacatgcac ggtgccggca cttcctgcaa
    30061 tccggatgct tttgcttatg cggctgctca ggcgaagaaa gctattgaag taaccgtccg
    30121 attaaacggt tccggttaca ctttttgggg aggccgcgaa gggtatgaaa cccttttgaa
    30181 taccaatatg ggtctcgaat tggataatat ggcacgctta ctgcatttgg cgacggatta
    30241 cggtcgaagc atcggtttta agggagcatt ttacattgaa ccgaaaccga aagaaccgac
    30301 aaagcaccaa tatgattttg acgcctcaac cgtaatcggt tttttgcgga aatatggcct
    30361 gaacatgaag ttgaatttgg aagcgaatca cggaacactg gccggacaca ccttccagca
    30421 tgaattacgg gtagcgcgga ccgaaggata tttcggctcg gtcgatgcca atatcggcga
    30481 tttgctgctt gggtgggata ccgaccaatt cccgaatgat gtctatgatt cgacttttgc
    30541 catgtatgaa attctcaaag cgggcggatt tacgaccggc ggtctgaatt tcgatgcaaa
    30601 agcccgccgt gcctcattcc agccggatga tatttttatc tcccatatta tgggcatgga
    30661 tacgatggca ctggggttgc gaatggctga caaattaatt cgagacggca gaatcgatca
    30721 attcgtggaa gatcgttatg ccggttataa gagcggcatc ggcaagaaaa ttgtcgacgg
    30781 aaccgtcaca attcaggaat tggaaaaata tgccttggaa atgggcgatg taaaaacaaa
    30841 taagagcggt cgccaggaat atttggaagg cgtcgttaat caaattctgt ttggataaac
    30901 tcatgaatta tctgatcggc attgatctcg gaaccagtgg gacaaaaacc gttctgtttg
    30961 atgagaacgg tcatgttcac ggttccgaaa ccatcgaata cccgctttat caggaacaaa
    31021 acggttgggg agaacaggaa ccggatgatt ggtggcatgc cgcatgcaac agtattcgat
    31081 ctgttattgc gcaaagtaaa ataaatcctt ctgatattaa aggcctgggc atttcgggcc
    31141 agatgcacgg attagtgatg ttggataaag acggtaaagt attgcgccgt tccattctct
    31201 ggcttgacca acggacagct aaggaatgtg aagaaattac cgcaaaagtg ggggcgaaac
    31261 gtcttatcga gataaccgcc aaccccgcac tgacgggttt taccgcctca aaaatattat
    31321 gggtgcgcaa tcatcaaccg gaaatttatg aaaaatgtgc tcatattctt cttccgaaag
    31381 attatgttcg ctataagttg accggcgaat tcgccaccga agtatcggat gcttccggaa
    31441 tgcagttgtt ggatgtaccc aaacgcaaat ggtccgatga agttttggaa aagttggata
    31501 tcgataaaag tttgttggga aaagtctatg aatcacagga gattaccggt ttggtcagtg
    31561 cccgtgccgc agccgaaaca gggctgaaag ccggaacacc cgttgtcggc ggagcgggag
    31621 ataacgcagc cgcggcggtc ggaaccggtg tcgtgcggga cgggttggcg ttcacgacga
    31681 tcggttcttc aggcgtcgtt tttgctcaca gctcggggat caaaatcgac ccgaagggcc
    31741 gggttcatac tttttgctgc gcggttcccg gagcatggca tgtgatgggg gttcatcagg
    31801 cagcggggct ttcattgaga tggttacgcg ataacgtctg tgcggaagaa gtcgcggaag
    31861 ctgaaaaacg ggggatcgat ccgtatgatg tgatgagtga aaaggcagat aaaattactc
    31921 ccggatcgga ccggctgatt tatttgccct atttgaacgg ggaaagaacc ccgcatttgg
    31981 atcccaattg ccggggcgta ttcttcggat tatccggtat tcataaacgg gctcacttaa
    32041 tccgtgcggt aatggaaggt gtcagctatt ccttgcggga tagtatcagt atcctgcgcg
    32101 aaatggacat cgaaaccagc gaaatgaccg cgaccggcgg aggcggcagg agctttgtct
    32161 ggcgacaaat gcttgccgat aattaccatt gcccggtgaa aacaatcgat aacaaagagg
    32221 gcccggcttt aggtgtagcg attcttgccg gagtcggcgt ggggttgttc agttcggtgc
    32281 cggaagcatg cgattcctta attcacgata aatccgtgca ggaaccgatt cgagccaatt
    32341 cggatgttta tgataaatat tatccgttgt atcagaaact ttatacggat ttgaaagatt
    32401 cattcaacga attggcgact ttataggagg ggaaataaat ctaaagtatt tcgagattaa
    32461 tgatagaatt aatatcgatt gattactttg gattttttca aaaatcgttt tttatatcaa
    32521 actggtaaat ccagcaaatt aagaaggaga gatcgatgaa aaagtttata tttgttttgt
    32581 tagctctggt agttttagct gtttcagtac cggtctttgc tgaaggtgaa ttgatcggtg
    32641 tggctatgcc caccaacagc ctgcagcgat ggaatcagga tgggaataac atgaagactc
    32701 agcttgaaaa agccggttac aaagttattc tggaatttgc gaataacgat gttgcgcttc
    32761 agatttccca gcttgaaaat atgattttga atgatgtgaa actgttggtc gtcgcttcca
    32821 ttgacggcgg tgctttgggt gatgtgctca agacggcgaa agataacgga attcctgtta
    32881 tcgcttatga tcgcttgatc accggaaccg aatacgttga ttattacact tcatttgata
    32941 attatcgtgt cggtaaattc caaggcgatt acatcgtaaa agcactggat ctggaaaatg
    33001 cagccggccc gttcaatatt gaattcttcg gcggttcacc ggatgataac aatgctcacc
    33061 tgttctatca gggtgcgata gacgttttgc aaccctatct tgattcaggg aaactggttt
    33121 gcccatccgg acagatcgat ttcaacaccg tcgcgattct cggctggggc actgatgatg
    33181 cacaggctcg tatggataac ctgatcaaca gctattatgc cgatggaaag accaaattgg
    33241 atgtggtgct ttctccgaat gactcactgg cgctgggaat tgccaactca ttaaaggcag
    33301 ccggcttcac actggacaat tggccgatca ttaccggtca ggactgcgat atccccaata
    33361 cgattaacct gattcaggga tatcaggcga tgtccgtgtt caaggacacc cgcacattag
    33421 ccgaacggac ggtaaagatg gcttcccaga ttctgaacgg ggaagaagtc gaagtcaaca
    33481 atacaaccga ttatgacaac ggtgtgaagg ttgtgccttc ctatctttgc gatccggttg
    33541 tcgttgataa gtcaaattac aaagagatgc tgatcgacag cggctattat accgaagatc
    33601 aaatagcggt taaataatcg tcaagcattg atagtttttt tgagggcagt gtgattttta
    33661 cactgccctc attcaaaaat acacttgtac aaaaccgcgg tcgcatatta aaataaaagt
    33721 tgaaaattcc ttgattctag cgaacgacaa tttttaccta ccggaacgac aacttttatt
    33781 tcaatatcca ccaagccctg tgaatcagga acctaatatt caaggagcgg aacaaatgga
    33841 gaaaattctt ctcgaaatgc aatctatcac caaggagttc ccgggtgtcc gtgcattgga
    33901 taatgtgact tttcaagtgc gtgaagggga aatccatgct ctggtaggag agaacggggc
    33961 gggtaaatca accttgatga atgttttatc cggttggtat cctcacggaa gctatgaagg
    34021 aaaaattatt tacgacggtg aagaatgtaa attcttcaat ctgaaagaca gtgaaaaact
    34081 cggtatcgta attatccatc aagaactggg gttgattccc tatttatcca tcgcggaaaa
    34141 tatgttttta gggaacgaaa ctgcgcatag ggtcgggcca ttcgaggtaa tcgattggaa
    34201 cgaatgccat aacaaggcaa ttatgatgct taatcgggtc ggtttggatg aaaatcctga
    34261 aacactggta aaagatatcg gcgtcgggaa acaacagatg gttgaaattg cacgggcact
    34321 tgcgaaaaat gtccgtttgt tgattatgga tgagcccacc gcttccttga atgatgcgga
    34381 cagtcaaaga ctgttgcaga tggttcaggg aatgaaggaa cagggaatca cctgcattat
    34441 tatttcacat aaattaaatg aagtggaaga gatcgcggat gcgattaccg tgattcggga
    34501 cggtgctacg attgaaacac tttataaggg aatagatgaa atcacccaaa gcagaatcat
    34561 caaggacatg gtcggccgtg aaatgacgga tcaatatccg aaacgcgtcc cgaaaatcgg
    34621 ccaaatcgga ttcgaggtga aagattggaa tgtctatcat ccgctttatg aaggcagaaa
    34681 agtaattgac aatgtctcct tcaatgttcg gaaaggtgaa attgtcggtt ttgcggggtt
    34741 gatgggttcg ggtcgtacag aacttgccat gagtattttc gggaaagcat atggcaagga
    34801 tatttccgga aaaataatta aagacgggaa agaagtggaa tttaaaaata tcgccgatgc
    34861 gattaatcaa ggtgtaatgt atctgacgga agatcgaaaa ggggcggggc tgaatctgat
    34921 cgatgatatt cggcataaca ttacgattgc gaatttgcaa gctatcagcg atcgttccgt
    34981 gattaatgaa aatgaggaaa tcaaagtcgc aaacgaatat cggaaaagat tgaatattcg
    35041 ttctaccggg attttgcaac ctaccggtac actttcaggc ggtaaccagc aaaaagtgat
    35101 gcttagccgc ggtttgtttt ccgatcctga tgttctcatt cttgacgaac cgactcgcgg
    35161 tatcgatgtc ggttcaaaat atgaaattta cacatttatg aaccagttgg tcgaagaagg
    35221 aaagtgcctg atattcattt cctctgaaat gcctgaactt ttaggaatgt gcgatcgcgt
    35281 gtacgtatta aatgagggaa gaataatcag cgaactgaaa ggcagcgaaa taactcagga
    35341 aagaatcatg gatgatatat taaccgatac cagaaagaga gcaaaaaatg gagcaaacag
    35401 caaaagtaac gaaaacgaaa attaatttcc gccaatatgg gatgatcatc gccctggtca
    35461 tcttggcact tttctttttt gtgacaacca atgggcgttt cggaaaaccg atgaatgtgt
    35521 ataacatcat tatgcagaac ggatatgttc tcgttcttgc gatcggaatg ttattgccta
    35581 ttttaacggg aagcattgat ttgtccgtcg gttcggtcgt cgccgttgtc agtgcttttg
    35641 ctggagttat gatgtacaac tggggaatgc cagtttggat cacgattatt atttgtatta
    35701 tttccggcat aatcatcggt ttatggcaag gcttttggat cgcaatggtc aatatgccac
    35761 catttattgc gacactcggc ggtatgttaa tctttcgcgg attaactttg gtgattttac
    35821 aagggaaaac attagccccg ttgccatctt cttatgtcca aatttcttcc ggatacgtca
    35881 aggattatat cgggaattta ttcggaatca aatccagttt aaatatcaca acaattgtcg
    35941 tagccgtcat tttttgtatt cttattttga tcggtcaatt aaattcaaga aaacagaaat
    36001 tgaaatacgg gtttgaagtt tcaagcgtcg gcagcgtttt cctgaaaacc gccgtcattt
    36061 cggctgcgat tttgttggtt ttctggcgtc tggctttgta taaagggatc ccttatgtcc
    36121 tgctgattgt cggggttttg gctctgttct ataattttat tttaaccaaa acgacactcg
    36181 gccgtcatat ctatgcggtt ggcggaaatg ccaaagcggc tgaattgtcc ggtatcgata
    36241 tccgcagagt taagtatatt atttatatca acatggcatt tatgagtgcc gttgccggta
    36301 ttgtattttc ggcgcgatta aattcagcca gcccattggc cggtcaggga tttgaaatgg
    36361 atgccatcgc cgcctgttac ttgggcggtg catccgcttc cggcggtatc ggtaccatca
    36421 caggcgcact ggtcggcggc ttgattatgg gaatcttgaa caacggaatg tcgttaatgg
    36481 gcatatccag tgatgttcag cagattgtta aaggtttggt ggttattttt gcggttgcct
    36541 ttgatatcct ctccaagaac aaatcatcca gataatcatt tcattgtcaa aaaagcggac
    36601 ggagtaattc tctccatccg cttttttata tcagtcagcc gttccggagg attttatgga
    36661 ttataagcag ttggctcggc aacttgtttt tctattacag ggagagacgg atcaggtcgc
    36721 gattttggca aatactgcgg ctttattgat gacggaattg gaaaatgtca gttgggccgg
    36781 tttttatttg gaaaaagaag gggaattgat cttgggaccc tttcagggaa aaccggcttg
    36841 tatccacatt ccgttaggca aaggggtttg tggaacagct tttgtcactc aggaaacatt
    36901 gcgggtaccg gatgtgcatg ctttttcggg acatattgcc tgtgattcgg agtcggcatc
    36961 cgaaattgtc attcctttat caaatcgatc gggcaagttg atcggtgtca tggatttgga
    37021 cagcaaaaag cctgaccgtt tttcggaaga cgatcaggcc ggtttggaga tgcttgcagc
    37081 ggctcttcgt gagtttctct aagg
//
LOCUS       DAOSMM010000018        33718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1334_length_33718_cov_15.961144, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000018 DAOSMM010000000
VERSION     DAOSMM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 33718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33718
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1334_length_33718_cov_15.961144"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PK898_04415"
     CDS             <1..1125
                     /locus_tag="PK898_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU19310.1"
                     /translation="QIVILTLIQVGGIGLMTIVSGAYILLNKRIGIQTRQLLMTSAGN
                     LQLSGVVRLIKRILFGTLLFEGTGTVFLATQFCPEMGIGKGIYYAIFHSVSAFCNAGF
                     DIMGFKGEYSSLISYQSNWIVNLTIILLIVIGGIGFYVWSDTVKNRFRFRRLSLHTQI
                     TLTTTAFLLAFGTLLYFFFEKNFSMQGMTTDNRILASLFMATTPRTAGFNTIDLTKLS
                     EPGNLLTMILMLIGAGSGSTGGGIKVTTFAVLMLNLFFMARGYENVTIFKKRLESNSF
                     KQAGSILCCYLLAVIAATMWICSQEIYSATDVAFECFSAIGTVGLSKGLPALMKPISK
                     IILILLMYGGRLGGISFLMIFTRQEKERPLKRVSKRILLG"
     gene            1141..1791
                     /locus_tag="PK898_04420"
     CDS             1141..1791
                     /locus_tag="PK898_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOU19311.1"
                     /translation="MKSFLVIGMGSFGRHLAEKMIELGNSVIAVDKNQSLIEEIADRF
                     PDSYYGDCTNPAVLGNLGVNNFDVCFVAIGEDFFASLEITSILKELGARYVVSKAKGE
                     RQSKFLRKIGADEVIYPEKEIAERLAVRFNADNIFDNYQLTDEYSIYEIPVLPKWINK
                     AIETLDVRNKYNVNIIAIKNENYLNPNPSPKYVFKEGDHIIVIGSSKNVFTLAGKK"
     gene            2015..3967
                     /locus_tag="PK898_04425"
     CDS             2015..3967
                     /locus_tag="PK898_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOU19312.1"
                     /translation="MDDHNLMEEDNLFHKKSDYKPSRSITTILIGKPLETAEQEHQTI
                     NKIIGLAVFASDALSSMAYSTQEMMLILAAAGTAAFGYVIPLTLCIVLLLVILTISYE
                     QTIHAYPNGGGAYIVARDNLGDYPAMTAAAALLTDYILTVAVSISSGVAQFTSVFPNL
                     YPNRVIIAVCLVLLVMVINLRGVKESGITFAIPTYIFLVSMGVMVIIGLIQYFTGSLG
                     QVVNPPALQHDGPFVAISTFLILKAFASGTTALTGVEAISNGITAFKEPRTKNAGQAL
                     MMMSVILGTLLMGVSFLTVKTGVIPSEMEANISQVARTIYHSKGIPYLITVASTMIIL
                     VMAANTAFADYPRLSAMLGRDGFLPRRFAWRGSRLVFSTGIMFLAGMAILLIVVFQAS
                     VNRLIPLYAVGVFVSFTLSQFGMAKRWHRVGKLKEGEELKSHGSTISYDKNWHFKMFA
                     NAIGAFCTLVISLIFGITKFTSGAWFVLVLTPFMVFIFSRIHKHYAKVAYKLSLKKGE
                     KTGTQIVRHRVLLLVSGVHQGTLKALRYARTISDDITAVHVAVDPEEAEKVMKGWQEW
                     GDGYRITFLESPYRVLIEPLVGYIESLLKKLNPNELITIVVPQFVSRDPAVSILHARA
                     ADTLRKALIHYQNVVIIEVPYNIDEL"
     gene            3998..4669
                     /locus_tag="PK898_04430"
     CDS             3998..4669
                     /locus_tag="PK898_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOU19313.1"
                     /translation="MNVIVIGCGKFGTSIASELEHAGYDVVVVDKDYQSFADLPDSFH
                     GRRINGDAMDLEVLERAKVKDADAVILTSQDDTTNLVLAHMFKNHFNIENVISLNTDP
                     EFRFYYDMTGNNTISMVSWGTLKIEEILMKKSKPSDDSIETTPIEIFNVVLPDIGRPY
                     SAIELQKVPGSIIFAITHDHITSKFLDKTEVKPGDILHMSSTMEGLRNFYDYLDQAAQ
                     KEKSE"
     gene            4666..5340
                     /locus_tag="PK898_04435"
     CDS             4666..5340
                     /locus_tag="PK898_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOU19314.1"
                     /translation="MNILIASAGRTSSQLAKVLLKQNHNVSVIESRPEVLQYVHKELP
                     TDAIFEGEILDPDTLEQAGIKDANVLVASTDDDKVNLLLCYIAKERYNVPRTIARVNN
                     SKHAWLFDEKFHVDYALDQATIFSMMIEEEISAGHMMVLLQLGKGDFSLVKEVIPADS
                     SVIGKKIMDLNLESVIAAIIRNGNVIPPHGDTVFMEKDEVIALTDREGLNQLRNLFSS
                     QSIDRK"
     gene            5580..6560
                     /locus_tag="PK898_04440"
     CDS             5580..6560
                     /locus_tag="PK898_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19315.1"
                     /translation="MKKISVSVLLFIMIMSVLIVHPVTAEDDIIEIGVLQLVEHASLD
                     AAYRGFVDALKEAGYEDGVNIKIDYQNAQGDQANLMTISERFVKNQVDLILAIATPAA
                     QSVASATTDIPILFTAVTDPVAARLVESNEAPGLNVTGTTDAGPIDKQLELLKELYPD
                     LKTLGIIYNSSEVNSEVQAKQAEEIAKNLGWEVKLGTITSVNDIEQVATSLAGKVEAF
                     YAPTDNTIASAMPNLAKVAEEHGLPIIAGAPEMVEAGGLATVGIDYYKLGAQTGKMAV
                     RILKGEAKPAAMPVESLKDVDVVLNKDYAKAIGYSFPQSVLDKATKIIGE"
     gene            6841..7818
                     /locus_tag="PK898_04445"
     CDS             6841..7818
                     /locus_tag="PK898_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19316.1"
                     /translation="MKKISALILFVLVVTALPLGIVSAEDAPIKIGVLQLVEHAALDA
                     AYQGFVDALKEAGFEDGVNIEIDYQNAQADQSNLMTISERFVKNNVDLILAIATPAAQ
                     AVASATTTIPILFTAVTDPVAARLVESNEKPGLNVTGTTDAGPIDKQFALMKELFPDL
                     KTIGIIYNSSEVNSEVQAKQAEEIAKEMGWEVKFGTITSVNDIEQVANSLAEKVDGFY
                     APTDNTIASAMPNLVKVAEEKKLPILGSEPGMVEGGALMTVGIDYFKLGQQTGKMAVK
                     ILKGEAVPAEMPVESLKDVDIVLNQATAKAIGFEFPESVLKKATKVIGE"
     gene            complement(7910..8842)
                     /locus_tag="PK898_04450"
     CDS             complement(7910..8842)
                     /locus_tag="PK898_04450"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOU19317.1"
                     /translation="MFELLFLGTSASAPSIKRNLPSLLVIHDEFRFLIDCGEGTQRQI
                     LTAGCGFKRLNKILFTHSHLDHILGTAGLLSTLLRWESMEDMDIYGGEDTIARVHDLL
                     FSVVLRGNRAPDFLRFHLLSEGILFDEKDFQVSCFPVNHRGSDSFGFRFDKKSRRPFL
                     PEKAEALGIPPGPWRRDLANEERVVLPDGRTIDPDQVLGDLIPGESLVVVGDTGETDS
                     LLPYVQHADALVIEATYTEEEADMAFQYSHLTAKHSARFAKTAGVGTLYLTHISRRYR
                     EKDILEEAQAIFPNTVVARDLAHYQIKKQAQTVK"
     gene            complement(9099..10427)
                     /locus_tag="PK898_04455"
     CDS             complement(9099..10427)
                     /locus_tag="PK898_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU19318.1"
                     /translation="MTEGNIIHQLLVFCWPLLVGNVFQQLYNTVNAILLGNYIGSDAL
                     AAIGVFNPINNLLLGLFSGISTGATVLVSQSYGAKNNEKLNQSVLVAIWMTILGGIFI
                     SIVGETVMNPLLRMIGTPAEVFTYAAEYSRIMFFGLVTMFFYNILSGILRGMGDSIIP
                     LLFLIFSNILNVGLLYFFIVILGTGIKGAAYGTVISETSAGILTFIYLLRSQEKYGIS
                     IKGLKPDWQIGKQMINIGLPAGLQTSIFSVGFLLQQNLINSFGPTVIAAYAVVTRIDQ
                     FAMLPMNSFAVGITAFVGQNTGARKMNRTIEGIKQTLILSQSLTIALVSILFFFGANV
                     MALFTNDLAVVQSGKLILRTLSAGYILVNAYTVLSGAVRGVGDTVAPLLASFICNVII
                     RVSLAYFLVKMTGNYQLIFVSISFSWALASAFLWIYYKKGFWQKKIRGII"
     gene            complement(10594..11334)
                     /locus_tag="PK898_04460"
     CDS             complement(10594..11334)
                     /locus_tag="PK898_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19319.1"
                     /translation="MNFDAIKFPVAGIAQKQIHFTDEIHNYAFSFASPYIYNEPRRYK
                     EKNAFLLGAYNPFNPELQVPEKIHSYFTVSDKKEPYRDKKLNLNSSKVQIFDESDQEI
                     GHMDVLFPRNRFFKFSVMFPNILYNASGEPVAFCHYQPALRRKCIYSFQSSFDGLYKE
                     IKQEAIRDLENQGIIRCHFTYDLVRRDSDARKMLETLFSSEKKSELIQNRINMIQKPN
                     SDEAALLYLTAMVAFFWNTMIELPLTEN"
     gene            11504..13987
                     /locus_tag="PK898_04465"
     CDS             11504..13987
                     /locus_tag="PK898_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19320.1"
                     /translation="MNLINGRNLGELPGLRIYTAPKKCDRSRINDVLMMVITVEGKSP
                     KVKDFEEWGAILSEAYFTARGSFTMGITAAVKQFVSYLDEKFPGILLPNVMLNIAVLR
                     DRTLLIGHSGPVNTTVVYADHVDNFSDATSVGVSQGTQGLRFFQIEIHSGDLILMCPR
                     APSGWTNEAILDATGESPLNVIRYLMDQANGNLQGVVIQVKTGRGEILYRYKPPVITT
                     VDLGYQEESETEGYPFEQPSKAMPRSSAGKTENAYRVNDQANENKKPLFRTRLPLEPF
                     PIDFNSSFQKRTDQFSEEKETMSQPDRVTEAEEIGEELKSEPAAVQPDEARPVENEPA
                     EELPPFLRPDKLQDELPETTIPDTKKTTLFEEEYIFREPLTEVKKKSEPELIPNSDVS
                     QSPSTKHQRQKSTRVAKKPKVEKKAKAKGVFSWKKFFLGLFFGILIPILVVAILFFTY
                     TGRSKDTAYRSGLRNAVETAKIAVQQTEPTLQRISWEKVLEYLKEAALYGNSPAAEDL
                     RKQALESLDKLDNGILIPYHYALQSPLPKGTNLVKLDSTNQFLYALDAATGSIFRLNI
                     LNNGLSLDSSFSCTPGTYQSSRESDSDEQISVGKLVDFVILPAESGSERVVMGIDNNA
                     QLLYCPSVGEKTAQKLESPGIGWLGVDAVYFTDRTLFILDAKNNSVWKVPYLGKSDFD
                     PETSSYFGADGPALKDIVDFVIYEQYSYLLRQNGTLIFCDYTGYSPLCSYVDTLTFYN
                     DSQSIDLTKKHVFQINMNRSPDTSIYLMDRENQSLLNTTIKLNLIRNLVPDRFEGDGD
                     GKVVTAFGFIGQFQVVWASQDQLFVGALN"
     gene            complement(14053..14730)
                     /locus_tag="PK898_04470"
     CDS             complement(14053..14730)
                     /locus_tag="PK898_04470"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19321.1"
                     /translation="MKNSGKKIRIFLIISLITLLMAGFVPAYQEETPIISIDVDALTA
                     KIEAALSKQTDQKIADLELRISSLEEKLKAYETRITTLEKLASLPTVTPAPKGTPTET
                     PIPTRTPNPTGYECSTELLSPYYYGQFNPGSEFMFQVRITNTGSKTWGNDVTIKYSDG
                     LKAEVEEHYAYALPVASVAPDESIEISIPMKAPTEKKNDGKYQSYYVLNNGKEDFCEF
                     SYSIYVP"
     gene            14969..15742
                     /locus_tag="PK898_04475"
     CDS             14969..15742
                     /locus_tag="PK898_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU19322.1"
                     /translation="MKKHVKIAIFLSLLITVIAGCSSSNQKASEKKYRVATDPVWPPF
                     EMVDEKTKEITGFDIDLMNAIAENQGFAVEYVNVNFDALIPGVGTCQYDAGASAITIT
                     DERKKQMLFSDPYVNIGQVMVVREDVNDLVNKEDFAGKVIGAQTGTTGAIMAEEWQTE
                     GLVTFRGYDTIDLAYMDLKNGQIDGVLGDNTMAEAYVKSMGGLKIVGDQYSSEELAFA
                     VCKDNKDLLDKINAGLKALKESGKFDEIYKKYFEQSVNK"
     gene            15929..16507
                     /locus_tag="PK898_04480"
     CDS             15929..16507
                     /locus_tag="PK898_04480"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOU19323.1"
                     /translation="MTQKNNTAYIILGLLTHKDSSGYDLKKEIDLKISRFWNIGYGQL
                     YPTLKILEEEGSIIGKPEKSEKGPDRIIYSITEKGKKKLTDWLSLPNEKEYVRYEILL
                     KLFFGNLSGPTNTIQRIETFQKQHQEDLQLMQSFKTNLESVFQENADHLYYYLTVLFG
                     EHIYKAYLDWADEALAFINKNLLSEENKKESS"
     gene            16504..17304
                     /locus_tag="PK898_04485"
     CDS             16504..17304
                     /locus_tag="PK898_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU19324.1"
                     /translation="MMKRQKIRKFLLLFSFLLFPVTLYYFSPVLIIQGSFSGIVTGSL
                     ILFAIQFVISLFWGRAFCGWACPGGSLQEYCSQVIDKKVSDKKTDRIKWIIWMPWIFT
                     IIFGFISQGGIKSVNFFYMTDHGISVSGITELVVYLFFVFLITVLALTVGKRGMCHSI
                     CWMAPFMILGSRIKNRFGYPSLHLEANSEKCVKCGVCSKNCPMGLNVEEMVRTNTLQN
                     DECILCGGCVDLCAKNVLRLTFKKAEKPNTKKAYILDTTEDPSKTVSI"
     gene            17324..17803
                     /locus_tag="PK898_04490"
     CDS             17324..17803
                     /locus_tag="PK898_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19325.1"
                     /translation="MIGHLGFSYVGLVYLLMLWIPNAIWSKNKPIDYRPDRENRVLLI
                     FERAGQVCCTCCVLIFTDLNITPFSGRSSWLIASFFSMILYELNWIRYFLNEHTEKNF
                     YRSFCGIPVPAASLPVLAFLLLGIYGNVIWLILSSILLGIGHIGIHIQHLRAIKKVL"
     gene            complement(18195..20516)
                     /locus_tag="PK898_04495"
     CDS             complement(18195..20516)
                     /locus_tag="PK898_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19326.1"
                     /translation="MKTKIFIYLFPIFLIFSIFSCASAEGLSDGFGMGSAIALRNNYS
                     EGMLEAYKTLNNAGISYVREEIIVDNISDLNNQKRMIEAARNQNLKIVGLLTYGFGFT
                     ENDDDFFEKWEDAVDKIVKELGYGIDYWEIGNEMNCIEFWKKVRPNAQTVEVNIYAEM
                     LNRAYKIIKKYDPDDRVILGGLIASADFKNGYDPADFLRNAEKYWDKPPFDAIGIHIY
                     WGAAFPDMITQNIIDKKIVTGNMADYIRLFNESIIEIFEKEYPIWITEVGYSNYWLSE
                     LYASYNHYEESTLQAIGLIRTYTMLLSTPPVEKVFWYTYSPDIEDVYVINDISLNVLR
                     TLYLAINDTMPLGRFRVMDTLGKERNDLCDYRFLQNDGQTISIFWSNDKEESSSSIKP
                     PLSSSIYLFDIENKTESQGIISFNEGTKFYILNIPIFIVGKMDNNESIVVTNDDTPVV
                     AENKPDVRPLIYTKEGNVYVYDPEKGVTRALTFDGGLSGGILYYNAMLSPDSKYVGVE
                     LSDSFMILDLQGNILYSWKKYSKPDIYADTLVGWDDQNYLYFTRTIGDCIFTDNEIIG
                     PDHVELVRIDPKTGESETLTNLAKLSNASHAYSIGDKISNDGKYISMINAACSIGISD
                     ERYYMNIETGDVIQESDYLKIYPDYDIQFPIEEENNYMIPGRILSPDHNYVAYIRFKY
                     SEYDGEYFVISEEADSRQLFIASTKDLQNKLFIDYADDLLGWSPDQTKLAYIRKEPGA
                     GGLYPPSTLYILDLTVGQIIQIDNGLGIKSSGW"
     assembly_gap    20738..20837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20838..21020
                     /locus_tag="PK898_04500"
     CDS             <20838..21020
                     /locus_tag="PK898_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19327.1"
                     /translation="YRNKGASKAVPGFDVIDYGQEDYVFGDKDSDARHWDTFVNKIFQ
                     SPEYAGVLANLFNEAK"
     gene            complement(21233..22027)
                     /locus_tag="PK898_04505"
     CDS             complement(21233..22027)
                     /locus_tag="PK898_04505"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOU19328.1"
                     /translation="MYKFEKAVVRTPADSFAEGITPGLFGKADPELAKIQHKKYIQAL
                     RQCGLEVIVLEPDNDFPDSCFVEDPVIVTDRVAILTNFHAPTRKGEVDRILPVIKDIY
                     GSHIERIEGPGTVEGGDICQVENHFFIGLSQRTNEEGACQLAAILKKYGFTSSIISIR
                     ALPEILHLKTCMSYIGENTFILQKAILKESELAPFKKIVVPDNEGYASNCIRVNDTVI
                     LPEGFDETIKQTRAAGFNVLETPMSEFMKQDGGLSCLSLRIPKLNL"
     gene            complement(22194..22916)
                     /locus_tag="PK898_04510"
     CDS             complement(22194..22916)
                     /locus_tag="PK898_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOU19329.1"
                     /translation="MISIRNVYKYYKDDIQALRGVSLDVYKGEVVMIIGPSGSGKSTL
                     LRSINNLEDIDFGEIIVDGLSVKDHKDINKLRMEVGMVFQQFNLFPHLSVLDNVMLAQ
                     KIVRKRSQKEAYETAMLLLEKVGIPDKASAYPLQLSGGQQQRVAIARALAMNPKIMLF
                     DEPTSALDPEMIQEVLEVMIQLAKEGMTMVVVSHEMGFARAAADRCILMDEGKIVEEA
                     PKDELFTNPKEERTKLFLSKIL"
     gene            complement(22930..23766)
                     /locus_tag="PK898_04515"
     CDS             complement(22930..23766)
                     /locus_tag="PK898_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOU19330.1"
                     /translation="MLSKKKNENDTNQWIDKDLPQVGELSKIDAWWGLVLIVLISIIL
                     LLVLKPDPFSRILDYVSDGIIVTLEVTVFSFIFTMVLGLFCGLGRVSTNKIIYGLVSV
                     YVEIIRGIPLLVQLLVWYFAFPAVVINIGKSMHYEPFENYLANPVAMAIIGISICYAA
                     YMAEIVRAGIESLPAGQMEAARSLGMTKSQAMQHIILPQAYRTIMPAVGNEFISLLKD
                     SSLVSVVAVADLTRRGREFMSTTFMPIETWLMVALLYLLMTLISARLVKILENKSKMG
                     GR"
     gene            complement(23907..25115)
                     /locus_tag="PK898_04520"
     CDS             complement(23907..25115)
                     /locus_tag="PK898_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU19331.1"
                     /translation="MHILLIHQAFVTVKEAGGTRHAELAHWLIQKGHQVTVITSPVSY
                     LTGENQPDIQKKPIDRIDGIDVYYCYTSGGLHKGFFPRVMSFFSFMFSSIKTAKKVQN
                     IDIVWGTSPNLFQAFAAWVIAKQQKKKFLLEIRDLWPDFAVGMGVLKNRFLIQASLWL
                     EKFLYAHADRIIVNSPGFIPHIQKISGKTALLVPNGADPDLFVSTDGGFSFRREHQLE
                     EKFVVMYTGAHGPANDLFTVLKAAELLREDDRIRIVLVGSGKEKDLLIKQKEEKGLNN
                     VLFVPPVPKEKIADVIAAADAGLAVLKAIPLFTTTYPNKVFDFMAAGKPVLCQIDGVI
                     RDLVEQYDAGLFVKPGDPQALKEAILLLAGNPEDCRRMGQNGIRTIREHFSRKKIAED
                     FERILFDLLI"
     gene            complement(25120..27114)
                     /locus_tag="PK898_04525"
     CDS             complement(25120..27114)
                     /locus_tag="PK898_04525"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOU19332.1"
                     /translation="MMNPISKKLKALRSFGIKRLWYYGTYTLKVKSGFFQKQSIPKNS
                     KNNKKTVVFSPLPNGRKDEILPFLLADSKSILESAGEIITVGYRPYSGNIIRSLDLVP
                     PHKDLFWTRIPSRENLDIKDIWEPARFCWVTALCRAYVLTGDRIYPEFFYRQVQTFIE
                     SNPPYYGENWVSAQEAAIRMISVTFAAGILQPSLPDKNSKFPDLPAFITLHATRIMQT
                     RSYAKAQNNNHRLSEAAGLMTAACVLPKSPDASKWFSTGWKDFQNALNSQILPDGAYI
                     QYSVNYHRLMLQLVLWVDSLLKTQKIAWPANLIRKIRRSVLWLAKHTDEFSGQACNTG
                     HNDGSLLFALGGNYNDFRPTLQTAAIVFLGKPLFPPGPWDEMRIWLGCYNRAGVDPDS
                     QFESTFYSQNVQRISAGNQWGILHAAGFKDRPAHADQLHVSLWKGGKCMALDAGTYRY
                     TAAPPWNNRLKSAFVHNCLTIDCQEPMIDAGKFLWLDWDQARILDQTEFRKIAEHSGY
                     KRLGLKHSRILEKTRQDWLAVDKVLPIRVPNSPRAPNTLFHGNLSEKKHLFRLHWLLP
                     EQKFEVLKENNHWCFLSKDVKIIFSANTIQLNLIIIRAGQQIFPTGSSAENSDPFRDI
                     CGWYSPTYGIHIPALSLILTAETAAPFVICSRWKFLPPGL"
     gene            complement(27155..29329)
                     /locus_tag="PK898_04530"
     CDS             complement(27155..29329)
                     /locus_tag="PK898_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU19333.1"
                     /translation="MKQLQQNMKNGKIAIKEIPVPEPESGFILIQNAYSLVSAGTERT
                     SADFAEKNLLEKAASRPDLTKQVIEKAKREGIVSSLESALNRLDIPMYPGYSSAGKII
                     AIGNGVTGFRIGDRVACGGGNYAIHAEYCTVPKNLVVKIPDHVPFEEAAFTTLGSVAM
                     HGIRLAVPQINETVLVIGLGLLGLLTIQISAAAGCRVLGIDISSQRVNLAQDLGCDAC
                     LRDEIDSVALPFTKGRGFDHVFICAGNHDNDTIELAGNECRDRGNVIAIGDVGLNIPR
                     KIFYNKEIAFRVSRSYGPGRYDPEYEEKGKDYPIGYVRWTEGRNMEAFIDLLAEGKVK
                     TAPLISHRIPIAEGEKAYEIIKGKTNEPYLGILIEYPFQNDETIFTKNTVQLREPSLP
                     QPVNLGVIGAGNYANATFLPILKSAGKSIRLVTIVSNRGASAREAAEKFGFEKAGVNA
                     ETVLNDPDINTVVILTRHNLHSSMVIEALNRQKNVYCEKPLALDFDSFQAVYQAKKAN
                     PKPILMVGYNRRFAPLAVKLKNFLADCAEPLAMHYTVNAGFIPLTHWTQDIEIGGGRI
                     LGECCHMLDFMLFLTQSRPLQVNTIALPNNGRYQDDNVSIQVKFENGSIGTVAYLANG
                     DKSYAKERLEVFGGGKIAILNDFKSLETWDRGNHHRTNSPLRIDKGHAASWQAFVSAI
                     INGEHEPIPTEQLFYSSLTSLAAVDSLHSNEIVAVPDLNIFL"
     gene            29517..30293
                     /locus_tag="PK898_04535"
     CDS             29517..30293
                     /locus_tag="PK898_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19334.1"
                     /translation="MKPFTEHFIKSWHITAVLMISCGLAAFVIAHFLPARYTATAFLS
                     FSIDHNQTGSLTDLEEDRMIGITEDIIKSDEVFAEVCRNMEGCTKESFLNRIQLDRTN
                     NQWYMSVSGEDPQESAAMASVWLDEAYTVLQRCLRAALEERGLKEVLNNLVLCFENSV
                     SGSLTNICPSDTAILFKKIEEISAEIMEKQRLAKGISPAIVLGEKRNVPGDIPVVRSN
                     ATTGFLTLLGLLIGFLISLLLCLMKPGVSKEPKTDCDPAE"
     gene            30300..31760
                     /locus_tag="PK898_04540"
     CDS             30300..31760
                     /locus_tag="PK898_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU19335.1"
                     /translation="MNPISFFEKQADSPIWKGIWFLFFFLLPITSMPAVVNLVHSDVV
                     AAPSGLILAVLLIGWVFPKLLSGEKFSENAIPLFCFVIVSLISTAVSFFYEIPIYKQS
                     DPLRSSISAIITVFIGAGFFIAVMLWVQDPQRLKLAMQALNWSGALVLIWTLMQAFFW
                     YTTFGYPQWMKDIQYAVSVGSLYRQRFVGFALEPSWLAHQLNLLYLPFWFAAAVTGYS
                     AHSKRFGKITFERILFVLGAVVLYLTLSRVGLAAFILSILFYFIWRIVHFAETQSAGT
                     KAPKKKKGLILLGFFVLIPFILLFILWTLTKLDFRMAELFTVNIKGRNNPLMYLAEKL
                     SLASRFSYWEGGLKIFSQFPILGVGLGHAGFYLPQNLSAFALRLVEVRDLLFRSEVLL
                     NIKSLWIRILAENGIIGFAFFVAWLVSTWGLSRRMMIRSSSLIKTIGWMGCFSAVAFL
                     LEGFSLDTFALPYLWVSMGLVGGVSLMPVPNGSSEK"
     gene            31786..33684
                     /locus_tag="PK898_04545"
     CDS             31786..33684
                     /locus_tag="PK898_04545"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOU19336.1"
                     /translation="MKKLMILIFLSTRFFLLLSPVQAEESEFNAASAEEMQERLESIQ
                     IDPADREEIARRFNHLTGELSPIPEPLNWQIGMDHLFWVINTDRKITVQIDAEAVYVG
                     EHIVIWVEKTALSQLSDAVYDQFKKFDFEIYPFEKYVFGSELSPGIDHDLRIHALFTK
                     KAGAGILGYFSSRDEDHPQVSPHSNAMELFILDSTMLAGNPDQITNTLSHEFQHMIHY
                     AHDANEGSNIDEGFSGLAEYLIHNRLSEAYERSFLENPDISLTLWPVSGSSIPYYGAS
                     FLFSKYLADRLGQDFLNKVVEQPRNGFEGIDAALKEVKSTFTADQLFAEWTAANLLYG
                     LGISNGEFSYRDYEPPLPKNGSMIKSLSCKNQSFDSTAMQYGTDYYNLPCDAGTYEIE
                     ITGQPTIPILSMNPIQGQYAWWSNSVNNSDTWMQHNFDLSNAKGSIRFEFDLNYDLEE
                     AYDFLYLSLSADGGKTWQMLKTAHGTDLNESGFNLGWGWTGKSGGWFHESVNLSEYSG
                     KNVFLRFDLITDQALTGEGVLLDNFRIDAIDFSDDVEKGSSGWEANGFLPIMNQLPQQ
                     YSDVLITGDSKKPAIFFHTTEGSETFSWTCDFEQQDKPICAFGISPINRISQLSAAYR
                     IQIKRNGP"
BASE COUNT         9440 a         6884 c         7507 g         9787 t
ORIGIN      
        1 caaattgtga tcctgacgct gattcaggtc ggagggatcg gcttgatgac gattgtcagc
       61 ggcgcttata ttctgttaaa taaaaggatc ggcattcaga ctcgccagct gctgatgaca
      121 tcggccggga atttacaatt gagcggtgtc gttcgcttga taaaacgaat acttttcggg
      181 actttattgt ttgagggaac ggggactgtc tttttggcaa cccagttttg ccctgaaatg
      241 gggatcggaa agggcattta ttatgcgatt tttcattcag tatccgcctt ttgtaacgcc
      301 ggatttgata tcatgggatt taagggagaa tattcttcct taatttctta tcaaagtaat
      361 tggatcgtca atctgaccat cattctgctg attgtcatcg gcgggatcgg attttatgtt
      421 tggagtgata cggttaaaaa tcgattccga tttcggcgtc tttctctgca tactcaaatt
      481 acattgacta cgactgcttt tcttttggcg ttcggtacac tcttatattt tttcttcgag
      541 aagaatttca gtatgcaggg gatgacaacg gacaatcgca ttctcgcctc attatttatg
      601 gcgacaaccc cgagaaccgc gggctttaat acaattgatc tgacaaaatt gtcggaaccc
      661 gggaatttat taaccatgat cttgatgtta atcggcgccg gctcgggttc aaccggcggt
      721 ggaattaaag tcacaacctt tgccgtcttg atgctcaatc ttttttttat ggcaagaggc
      781 tatgagaatg taacgatatt caaaaaaagg ttggaatcga attcttttaa acaagccggt
      841 tccatattgt gctgctatct tctggcagtt attgcggcta cgatgtggat ttgttcgcaa
      901 gaaatttaca gcgcgacaga tgttgctttt gaatgttttt ctgcgatcgg cacagtcgga
      961 ttatcaaagg ggctgcctgc tcttatgaaa ccgatttcca aaatcatttt aattttattg
     1021 atgtatggcg gaagattggg tggaatctcg ttcctgatga tttttaccag acaggaaaaa
     1081 gagcgaccgc tcaaacgcgt ctcgaaaaga atattattag gataagggac gggggaaaaa
     1141 atgaaatctt ttttagtcat cggtatggga tcattcggtc gccatctcgc cgagaaaatg
     1201 atcgaactcg gcaatagtgt cattgcggta gataaaaatc aaagcctgat tgaagagatc
     1261 gccgatcgat ttcccgattc ttattatggc gattgtacca atccggcggt tctcggcaac
     1321 ttgggtgtaa ataatttcga tgtctgtttt gtggcgatcg gcgaagattt ttttgcttcg
     1381 cttgaaatta cttcgatctt aaaggaactg ggagctcggt atgtcgtttc aaaagcaaaa
     1441 ggagaacggc agtcgaaatt tttacgaaaa atcggagcgg acgaagtgat ctatccggaa
     1501 aaagaaatcg ctgaaaggtt ggcggttcga tttaatgcgg ataatatttt tgataattat
     1561 caattaacgg atgaatattc catttatgaa attccggttt tacccaaatg gatcaacaag
     1621 gcgattgaga cattggatgt tcgaaataaa tataatgtga atattattgc gataaagaac
     1681 gagaattatt tgaatcccaa tccgagccct aagtatgtgt ttaaagaagg cgatcacatt
     1741 attgttatcg gcagttcaaa aaatgttttt acgttagcgg ggaaaaaata attctcatct
     1801 gtcgtgatat ttattttccg acttttgtcg atatggtcat cctgtgaaga atttgtcccg
     1861 ctcggatttt cctaaacggg ataaataaag attcactgga ttattcgatt ttgtcattta
     1921 agcggttccc tattgtataa ttgactcctg cttaaggatc gatgtattca aatgaagaat
     1981 gatttgtttt taacttattt gaggatataa aagcatggat gatcataatc ttatggaaga
     2041 agataattta ttccataaaa aatccgatta caaaccttcc cgttccatca ccacaatttt
     2101 gatcgggaaa cctctcgaaa cagctgaaca ggaacaccaa accattaata aaatcatcgg
     2161 attggcggtt ttcgcttcgg atgctctgtc atcaatggca tacagcactc aagagatgat
     2221 gttaattctt gccgcagccg gtacggcagc tttcggctat gtgattccgt tgacactctg
     2281 cattgttttg ttattggtga tcttgacgat ctcctatgaa caaacgattc atgcttatcc
     2341 gaacggcggc ggagcctata ttgtggcgcg tgacaattta ggcgactatc cggcgatgac
     2401 cgcggcagcg gctctgttga ccgattacat tcttacggta gcggtttcca tttcttccgg
     2461 ggttgctcag tttacttcgg tatttccgaa tctttacccg aaccgcgtaa ttatcgcggt
     2521 ttgcttggta cttttggtga tggtaatcaa tttgcgcggg gttaaggaat cggggattac
     2581 ttttgcgatc ccgacgtata tattcctggt ttcgatgggc gttatggtga tcatcggatt
     2641 aatccagtat tttaccggtt ctctgggtca ggttgtgaac cctcccgcct tacagcatga
     2701 cggtccgttt gttgcgattt cgactttcct gattttaaaa gctttcgcga gcggaacaac
     2761 cgctttgacc ggtgtggaag cgatttcgaa cggtattaca gcgtttaaag aaccgcggac
     2821 gaagaacgcc ggccaagcat taatgatgat gtctgtcatt ttgggaacac tcctgatggg
     2881 agtctccttt ttgaccgtca aaaccggtgt tattccttcc gaaatggaag caaatatctc
     2941 ccaggttgca aggacgatct atcattcaaa aggaatccct tacctgataa cggtcgcttc
     3001 gacgatgatt attctggtaa tggctgccaa taccgctttc gcggattatc cgagactcag
     3061 tgccatgctg ggacgggatg gttttttgcc gcggcggttt gcatggcgcg gatcccgcct
     3121 ggttttttcc acgggcatta tgtttttggc gggaatggcg attttattga ttgtggtatt
     3181 ccaggccagt gtgaaccggt tgatcccgct ttatgcggtc ggtgtttttg tttcctttac
     3241 actttcgcaa tttgggatgg cgaaacggtg gcacagagtc ggaaaattga aagagggcga
     3301 agagctgaaa agtcacggtt cgacgatcag ctacgataaa aattggcatt tcaaaatgtt
     3361 tgccaacgcc atcggagcat tctgcacact ggtgatttca ttaattttcg gaattacaaa
     3421 atttaccagc ggcgcttggt tcgttttggt cttaacccct ttcatggttt ttattttttc
     3481 cagaattcat aagcattatg ccaaagtagc ttataaactg tctcttaaaa aaggagaaaa
     3541 aacaggtact caaattgttc gccatcgagt cttattattg gtaagcggtg tccatcaagg
     3601 aactttgaaa gccctgcgat atgctcggac gatttcggac gacatcactg cggttcatgt
     3661 cgcagtggat ccggaagaag ccgaaaaagt catgaaaggt tggcaggaat ggggagacgg
     3721 atatcggatt accttcctgg aatcacctta tcgggtcttg attgagccgc tggtcgggta
     3781 cattgaatct ctgttgaaaa agctgaatcc caacgaatta attacgattg ttgttccgca
     3841 atttgtcagt cgcgatccgg cggtttccat cttgcatgcc cgagcggcgg atactcttcg
     3901 aaaagctttg atccattatc aaaatgtggt gattatcgaa gtaccttata atattgatga
     3961 actatagatt attccttcgg aggagagaga ttttttcatg aatgtcattg tgatcggctg
     4021 cggaaaattt gggacaagca ttgcctcgga actggagcat gcgggttatg acgttgtagt
     4081 cgtcgataag gattatcaaa gttttgcaga tttgccggat tcctttcacg gacgacggat
     4141 taatggcgat gccatggatc ttgaggtctt ggaacgggca aaagtgaaag atgccgatgc
     4201 cgtaattctg acttcgcaag acgatacgac gaatttggtt ctggctcata tgttcaaaaa
     4261 tcattttaat atcgaaaatg tcatctctct gaataccgat cccgagtttc gtttctacta
     4321 tgatatgacg ggaaataaca ccatttcaat ggtatcgtgg ggaactctca agatcgaaga
     4381 aattctgatg aaaaaatcca aaccgtccga tgattcgatt gaaaccacgc cgattgaaat
     4441 ttttaatgtt gttttgcccg atatcggaag gccatattca gcgatcgagc tgcaaaaagt
     4501 tcccggaagc atcatttttg cgattacaca tgatcatatt acgtcgaaat ttttagataa
     4561 gaccgaagta aaaccgggag atattcttca tatgagctcc accatggagg gattgcgtaa
     4621 tttctatgat tatcttgatc aggcggctca aaaggagaaa tccgaatgaa tatattaatc
     4681 gcttcagccg gcaggacctc atctcaattg gcgaaagtgc tattaaaaca aaatcacaac
     4741 gtatctgtga tcgaatcacg cccggaagtt ttgcaatatg ttcataagga attgcccacc
     4801 gatgccattt ttgaagggga aatattagat ccggacacgc ttgaacaagc cggaatcaag
     4861 gatgcgaatg ttttggtggc aagtacggat gatgataaag tcaatttgtt gctttgctac
     4921 atcgctaagg aacgttataa cgttcctcgg accattgcgc gggtaaataa ctccaaacat
     4981 gcctggctgt tcgacgaaaa atttcatgtc gattatgcct tggatcaggc gaccattttc
     5041 tccatgatga ttgaggagga aatttcggcc ggtcatatga tggtactgct tcaactcggc
     5101 aaaggcgatt tttcattggt taaggaagtt attccggctg attcttcggt gatcggaaag
     5161 aaaataatgg acttgaattt ggaaagcgtg atcgctgcca ttattcgaaa tggaaatgtg
     5221 attccccctc acggtgatac ggtttttatg gagaaggatg aagtaattgc cttgaccgat
     5281 cgggaaggtt tgaatcaatt acgcaatttg ttcagttcac aatcgatcga caggaaataa
     5341 gcgattgcta tggtcatgtc attaagcatg tcatgatttt caaaataagg gttgaaaacg
     5401 aaaacggaat catgttataa aatgggtatg caaaatattc actcgttttt cttctttttc
     5461 ttttttgcca cacctcacaa agttgtgcag cgtgaatttt ttgcataaat caatccggta
     5521 gcaaaaagac ctcctgcaca acaggaggtc tttttatttt atggttcagg agtcatacaa
     5581 tgaaaaaaat atctgtgtcg gttcttttat tcatcatgat tatgtctgtt ttgatcgttc
     5641 atcccgtaac ggctgaagat gacataatcg aaatcggtgt gttgcaattg gttgaacacg
     5701 catcacttga tgcggcttac cggggatttg tcgatgctct caaggaagcc ggctatgagg
     5761 atggggtcaa catcaaaatc gattatcaaa atgctcaggg ggatcaagcc aatctgatga
     5821 cgattagcga acggtttgtg aaaaatcagg tggatttgat tttagccatt gccacccccg
     5881 ccgcgcagtc cgttgcttcc gcgactacgg acattccgat tctgttcacc gctgtcacgg
     5941 atccggttgc ggctcggtta gtggaaagca atgaagctcc cggtctgaac gtcaccggca
     6001 caaccgatgc cggcccgatc gataaacagc ttgagctgtt gaaggaactt tatcccgatt
     6061 tgaaaacttt ggggatcatt tataattcct ccgaggtgaa ttcagaagta caggcaaaac
     6121 aagcggaaga aattgcgaaa aatctcggct gggaagtcaa actcggcaca atcacttcgg
     6181 taaatgatat cgaacaagtg gcaacctctc ttgcgggtaa ggtagaagct ttctatgcac
     6241 cgacagataa cacgatcgct tctgccatgc caaatctggc aaaagttgcg gaagaacacg
     6301 gtttgccgat cattgcgggg gctccggaga tggtcgaagc gggcggtttg gccaccgtcg
     6361 ggatcgatta ttacaaattg ggtgcacaaa ccggaaagat ggctgtccga atcctaaaag
     6421 gcgaagccaa accggctgcc atgccggttg agtctctcaa agatgtggac gttgttttga
     6481 ataaggatta tgccaaagcg atcggataca gcttcccgca aagcgtgttg gataaggcta
     6541 ccaagatcat cggagaataa gaacaagaga tgatttgaga tgatgattac cgatttcggc
     6601 aatcatcatc tcaaaaaatg gatgtcatca atttgttaaa atcatcttgc ataaaaatcg
     6661 aaaaatgtgc tataaaaaag ccatgcaaaa aacgaatcgt tcctattatt tttacggtta
     6721 ttattacgga aaatcacccc attcggtgtg gcgtgaagtt tttgcataaa tagaatcgta
     6781 agcaaaagac ctcctgcaca ccgggaggtc tttttttatt ctataaccag gagaaagatc
     6841 atgaaaaaaa tatcggctct cattttgttt gttcttgtag ttacggcttt gccgttgggg
     6901 attgtttcgg ctgaagatgc tccgattaag atcggtgtat tgcagttggt tgaacatgcg
     6961 gctttggatg cggcttatca aggatttgtg gatgccctca aagaagccgg ctttgaagac
     7021 ggggtgaata tcgagatcga ctatcagaat gcacaggcgg atcaatcgaa cctgatgaca
     7081 atcagtgaac gtttcgttaa aaacaacgtg gatttgatcc tggctatcgc tacaccggct
     7141 gctcaggcgg tggcctccgc gacaacaacg attccgattc tttttacggc ggtaaccgat
     7201 ccggtagcag ctcgcttggt cgaatcgaac gaaaaaccgg ggttgaatgt gacgggaacc
     7261 accgatgcgg ggccgattga caaacagttt gccctgatga aagagttatt cccggatttg
     7321 aagacgatcg gcattattta taattcttcc gaagtcaatt ccgaagtcca agccaaacag
     7381 gctgaagaaa ttgccaaaga aatgggttgg gaagtaaagt tcggaacaat cacttcggtg
     7441 aatgacattg aacaggttgc caactctctc gcagaaaagg tagacggatt ttatgcgccg
     7501 acggataata cgattgcctc ggcgatgccg aatcttgtaa aagtggctga agagaaaaag
     7561 ctgcccattt tgggaagcga acccgggatg gttgaagggg gcgcattaat gaccgtcgga
     7621 atcgattatt ttaaactggg tcaacaaacg ggtaagatgg ctgtaaagat tttgaaaggg
     7681 gaagcggttc ccgcagagat gcccgttgag tcgttgaaag atgttgacat tgtgctgaat
     7741 caagccacag ccaaagcaat cggtttcgaa tttccggaaa gcgttctcaa aaaagccacc
     7801 aaagtcatag gcgaatgaga acatctcttc agaaagaatc cccgcttttt cggggattct
     7861 tttttaacga acagactctc agacgttatt ttgtgccgag acgtggaaat tactttacgg
     7921 tttgggcttg cttttttatt tgatagtgcg ccaaatcacg ggcgacaacg gtattgggaa
     7981 agatcgcttg cgcttcttcc aaaatatctt tttcgcggta tcgtctggaa atgtgggtta
     8041 aatataacgt tcccacaccg gctgttttcg caaaccgggc agagtgtttg gcggtcagat
     8101 gagaatactg gaatgccatg tctgcttctt cttccgtata ggtggcttcg atgaccagcg
     8161 catccgcatg ttgaacgtaa ggcaataacg aatcggtttc tcccgtatca ccgacgacga
     8221 ccagactttc tccgggaatt aaatcaccca gtacctgatc cggatcaatc gttcgtccat
     8281 ccggtaggac cacgcgttct tcatttgcca aatcacgacg ccaagggccc ggcgggattc
     8341 ccaatgcttc cgctttttcg ggaagaaacg gccggcgcga ttttttatcg aagcgaaagc
     8401 cgaacgagtc cgaaccacga tgatttaccg gaaaacagga tacttggaaa tctttttcat
     8461 cgaaaagaat cccttcggaa agcagatgaa atcgcaaaaa atcgggagca cgattcccgc
     8521 gcagaacaac ggaaaaaagc aggtcatgga cgcgggcgat tgtatcttct ccgccgtaaa
     8581 tatccatgtc ttccatgctt tcccagcgaa ggagcgttga aaggagtccg gctgttccca
     8641 aaatatgatc cagatgactg tgagtaaaaa gaattttatt caatcttttg aaaccgcatc
     8701 cggcggtgag gatttgtcgt tgagtgcctt ctccgcaatc gatcaaaaaa cgaaattcgt
     8761 catgaattac caataaagac ggtaagtttc gtttaatgga gggcgcggaa gccgaagttc
     8821 ctaagaaaag taattcaaac aaattggttt atgttttggc ctcaatttct gtcagatcga
     8881 agttatttta ccagacagtg ggcaaagttc tcgacgtttt cgggcggata tcatacgaag
     8941 cggatttgat tccggcagat ttcgctgaat taccgatgcg gaggtcatgt acgctcatga
     9001 aattgacttg acttttattt tccgatacgc tttaattaag agtggcggac agcttatggg
     9061 tgaccccctc atccataatg tctgatccgc catgtctttt aaataatgcc gcgtattttc
     9121 ttctgccaaa aacccttttt ataataaatc caaagaaaag cactcgctaa agcccaggag
     9181 aaagaaatgc tgacaaaaat cagctgataa tttccggtca tcttgacaag aaagtaagcc
     9241 agggaaacgc ggataatcac attacagata aaacttgcca acagcggtgc gaccgtatct
     9301 ccgactccgc gaaccgctcc gcttaagacc gtataggcgt tgaccaagat atagccggcc
     9361 gaaagtgtcc gcaaaatcaa ttttccgctt tggacgaccg caaggtcatt tgtaaaaagc
     9421 gccatgacat ttgcgccgaa gaagaaaaga atggaaacca aagcgatggt caacgattgg
     9481 ctgagaatta atgtctgttt aattccctca atcgtacgat tcatctttct ggcacctgtg
     9541 ttttgcccga caaaagcggt aattccgacc gcaaatgagt tcatcggtaa catggcgaat
     9601 tgatcgatcc gtgtcacaac cgcataggcg gcgataaccg tcggaccgaa actattaatc
     9661 aggttttgtt ggagcaaaaa accgacggag aaaatggaag tttgcaaccc cgctggtaaa
     9721 ccgatattaa tcatttgttt cccgatttgc cagtccggtt tgagcccctt aatggaaatg
     9781 ccgtattttt cttgggatcg cagcaaataa ataaatgtta atattccggc agatgtttcg
     9841 gatattaccg ttccgtaagc cgcgcccttg ataccggttc caaggatgac gataaagaaa
     9901 tataacagcc cgacattcag gatattggaa aaaatcagaa agagcaacgg aataatggaa
     9961 tcgcccattc ctctcagaat tcccgaaagg atattataga aaaacatggt gaccaatcca
    10021 aaaaacataa ttctggaata ttcggcagca taggtaaaaa cttccgcagg tgtcccgatc
    10081 attctcagaa gaggattcat aaccgtctca ccgacgattg aaatgaaaat cccccccaag
    10141 atggtcatcc agattgccac cagaaccgat tgattcaatt tttcgttgtt ttttgcgccg
    10201 taactttgtg aaaccaatac ggttgcgccg gtcgaaattc cgctgaatag gcccaataaa
    10261 aggttattaa tcgggttgaa aacaccgatc gcagcgagtg catcactgcc gatatagttt
    10321 cccaatagaa tcgcattaac cgtattataa agttgttgaa aaacgttccc gacaagaagc
    10381 ggccagcaga aaacaagcag ctgatgaata atgtttcctt ccgtcatatc agcttgtcgg
    10441 tttcttgtca gttgtacaga tgttcgttcg gaagacttcg tttgtgacct tttctgtttc
    10501 aaatggtttg tgaaaatcgg ggctgtttcc ggcatattga tataactgtt tctcttatat
    10561 gaaggacagt aaataattta ttaataaatg attttaattt tccgtcaacg gtaattcaat
    10621 cattgtgttc cagaaaaatg cgaccatagc tgtcagataa agcaaagccg cttcatccga
    10681 attcggtttc tgaatcatgt tgattctgtt ttgaatcaat tcggatttct tttcggaaga
    10741 gaataacgtc tccaacattt ttcgcgcatc cgaatctcgc cggaccagat cataagtgaa
    10801 atggcagcga ataattccct gattttcaag atctcggatc gcttcctgtt tgatttcttt
    10861 ataaaggccg tcgaaagagg attgaaaaga ataaatgcat tttctgcgca atgccggttg
    10921 gtaatggcaa aatgccacag gctctccgga tgcattatat aaaatattgg gaaacatcac
    10981 gctgaattta aaaaaacggt tgcgggggaa aaggacatcc atatgtccga tttcttggtc
    11041 cgactcatcg aaaatctgaa ctttgctgct gtttagattg agttttttat cccggtaggg
    11101 ttccttttta tcgctcaccg taaaatagga gtgtattttt tccgggactt gcaactccgg
    11161 attgaacgga ttataagcac ccaaaagaaa agcatttttc tctttatacc ggcgcggctc
    11221 gttgtaaata taggggctgg caaaagaaaa agcgtaatta tgaatttcat cggtaaaatg
    11281 aatttgcttt tgggcgattc ctgctactgg gaatttaatg gcatcaaaat tcatggcatc
    11341 tccgcatttt ccaaaaatga caacattgta cacttgatta tacacggatt ggaatttcga
    11401 ttcggatgaa ttctggcttg aaaattgcac atttaatgac aagtctgata tttgtgaaag
    11461 cctgataact ggtgatgatt tgttcgattt aaatattctc ccaatgaatt taataaacgg
    11521 tcgaaatctc ggtgaattac cgggtttgcg catctatacc gctcccaaaa aatgtgaccg
    11581 atcacggata aacgatgttt tgatgatggt cataaccgtc gaaggaaaga gcccgaaagt
    11641 caaagatttt gaagaatggg gagcaattct ttcggaagct tattttacgg cccgtggatc
    11701 ttttacgatg ggaataacag cggccgtaaa acaattcgtt tcttatttgg atgaaaagtt
    11761 tcccggaatt cttctgccga acgttatgtt gaacattgcc gttttacgcg atcgaacgct
    11821 gttgatcggc cattccggtc cggtgaatac gaccgttgtc tatgcggatc atgtggataa
    11881 tttctcggat gcgacttccg tgggcgtttc tcagggaacg cagggactga gattcttcca
    11941 aatcgaaatc cattcgggag atctgatttt aatgtgtccc cgcgccccat ccggatggac
    12001 aaatgaggcg atattggatg ccaccggcga atctccgttg aatgtaatcc gttacttgat
    12061 ggatcaggcg aacggaaatc tgcaaggtgt tgtgattcaa gttaaaacag gacgcggcga
    12121 aattctctat cgttataagc cgccggttat tacgaccgtt gatttgggat atcaagaaga
    12181 atcggaaaca gaagggtatc ccttcgagca acccagtaaa gcaatgcctc gatcttcggc
    12241 gggaaagacc gagaatgcat accgtgtgaa cgaccaagcc aatgaaaata aaaagccgct
    12301 atttcggacc cgtttacctt tggaaccttt cccgattgat ttcaattctt cttttcaaaa
    12361 aagaacggat caattttcag aggaaaaaga gacgatgtct caaccggatc gggtgaccga
    12421 agcggaagaa atcggtgaag aattgaaatc cgaacccgcg gcggtgcagc cggacgaagc
    12481 gagacccgtt gaaaacgaac cggcggagga gcttccgccg tttctccggc ccgataagtt
    12541 gcaggatgaa ctgcccgaaa caacgattcc ggatacaaaa aagacgacac ttttcgaaga
    12601 agaatatata ttccgagaac cgcttacgga agtaaaaaaa aaatccgaac cggaactgat
    12661 tccgaattct gacgtttctc agagtccgtc gacaaagcac caaagacaaa aatccacaag
    12721 agtcgcaaaa aaaccgaaag tagaaaagaa ggcgaaagcg aaaggagttt tttcctggaa
    12781 aaaattcttt ctcggattgt ttttcggcat tctgattccg attctggttg tggcgatttt
    12841 gttctttact tataccggac gcagcaagga taccgcgtat cgttccggtt tgcggaatgc
    12901 ggtggaaacg gcaaaaattg ccgttcagca aacagagccg actttacagc gaatttcatg
    12961 ggaaaaagtg ctggaatatc tgaaagaagc agccctctac ggaaattctc ccgctgccga
    13021 agatttgaga aaacaagctc tcgagtcttt ggataaactg gacaacggaa tactgattcc
    13081 gtaccactat gcgttgcaaa gtccacttcc gaaaggaacg aatctggtaa aacttgacag
    13141 cacaaatcaa ttcttatacg ctttggatgc cgctacgggc agcatttttc gtctgaatat
    13201 cctgaacaac ggcctctctt tggattcgtc gttttcgtgc acaccgggaa cttatcaatc
    13261 gagtcgggaa tcggattcgg atgaacaaat ttctgtggga aaattggttg attttgtgat
    13321 tcttcccgcc gaaagcggaa gcgagcgcgt cgttatggga atcgataata acgcacaatt
    13381 actttattgt ccttcggtcg gcgaaaagac cgcccaaaaa ttggaaagcc cggggatcgg
    13441 ttggctgggt gtcgatgcgg tttattttac cgatcgaaca ttatttattt tggacgccaa
    13501 gaataattcc gtttggaaag ttccttatct cggcaagagt gattttgatc ctgagacatc
    13561 ttcttatttc ggcgccgatg gaccggcgtt aaaggatatc gtcgattttg tgatttatga
    13621 acagtattct tatttgctca ggcaaaatgg aacactcata ttctgtgatt atacgggtta
    13681 ctcgcctctc tgttcttatg tggatacgct gacattttat aacgattcgc aaagcattga
    13741 tctgacaaaa aagcacgttt ttcaaataaa catgaatcgt tctccggata cgtcaattta
    13801 tttgatggat cgagagaatc aatcgctttt gaataccaca atcaaattaa atttgattcg
    13861 taatctcgta ccggatcgct ttgaaggaga cggggacgga aaggtggtta ctgcttttgg
    13921 atttatcggt caattccagg tggtgtgggc atctcaagat cagctttttg tcggtgcgtt
    13981 gaattaaacg aatcataaaa gggaaatgat aagaacattt cccttttatg attcgtcgga
    14041 tcgtacgaat gcttacggaa cgtatatact ataactgaat tcgcaaaaat cttcttttcc
    14101 gttattcaga acataatagg attgatattt cccgtcattt ttcttttccg tgggagcctt
    14161 catcggaatg ctgatctcaa tggattcatc cggcgcgacc gaggctaccg gcagcgcata
    14221 agcataatgt tcttcgactt ccgccttcaa accgtccgaa tatttaattg tgacatcatt
    14281 tccccaagtc ttggaaccgg tgttggtaat tcgaacctgg aacataaatt cggagcccgg
    14341 gttgaactgt ccgtaataat atgggctgag gagttcggtt gaacattcat atccggtcgg
    14401 attcggagta cgcgtgggta tgggcgtttc cgtgggagtt cctttgggcg cgggtgtgac
    14461 cgtcggcagg ctggctaatt tttccagtgt ggtgattctg gtctcataag ctttcaattt
    14521 ctcttcgaga gaagaaattc gaagttcaag atcagctatt ttttggtctg tttgtttgga
    14581 aagggctgct tcgatttttg cggttaaagc atccacatcg atggaaatga ttggagtttc
    14641 ttcctgataa gccggtacga agccggccat cagcaacgtg atcaacgata ttatcagaaa
    14701 gattcgtatt ttttttccgg aatttttcat aatctactcc tttttgtcaa aagtaaaatc
    14761 caagttaatc ttagcatatc atatttatcg gtttgacaaa aatatttata aaataattcg
    14821 ttgaaaattc aattaataaa atcggataaa ggacaaaatc taccgtcctc ccccaaaaag
    14881 tttgtcataa atttataaag attatttttt ctatgctaaa atccgataaa tttaatccaa
    14941 cagcttttta ttcaaagagg agaatattat gaaaaagcat gtaaaaatag ctatctttct
    15001 cagtttatta atcacggtta tcgccggatg ttcttcaagc aatcagaaag catcagagaa
    15061 aaagtaccga gtcgcaacgg atccggtttg gccgcctttt gaaatggtgg atgaaaaaac
    15121 gaaagaaatt accggtttcg atattgactt gatgaacgct attgccgaaa atcaaggttt
    15181 cgccgttgaa tatgtcaacg tgaatttcga tgcactgatc ccgggtgtcg gaacttgcca
    15241 atatgatgcc ggtgcgtctg cgattacgat taccgatgaa cggaagaaac aaatgctttt
    15301 ctccgatccc tatgtcaata tcggtcaggt aatggttgtc agagaagatg tgaatgatct
    15361 ggtgaataaa gaagattttg ccggaaaggt gatcggtgct caaaccggaa caaccggcgc
    15421 tatcatggct gaagaatggc agacggaagg gttggttaca ttcagaggct atgacacaat
    15481 cgatctggct tacatggacc tgaaaaacgg tcaaatcgac ggtgttttgg gcgacaatac
    15541 aatggcggaa gcctatgtga agtcgatggg cggattaaaa attgtcggcg atcaatattc
    15601 atccgaagaa ttggctttcg cggtctgtaa agataacaaa gatttactgg ataaaatcaa
    15661 tgccggcttg aaagcgttga aggaatccgg aaaattcgat gaaatctata aaaagtattt
    15721 cgaacaatct gtaaataaat aatgtaaaag ccgcaccaat cgagcggaag atcgggaaag
    15781 tttttggctt tcccacagtt gggtcggaaa gatatcaacc gatatctttc cgaccttttt
    15841 ttcatccgaa aggaattcag tgaaaacact cttgacataa ctatatcaat ttgatatagt
    15901 tatgtcagat tttcaaagag gttgagaaat gactcaaaaa aataatacgg cttatatcat
    15961 actcggactg ttgacccata aagattcaag cgggtatgat ttgaaaaaag agatcgatct
    16021 taagatttca aggttttgga acatcggtta cggtcagttg tatcccacat tgaaaatatt
    16081 ggaagaagaa ggttcaatta tcggcaagcc tgagaaatcg gaaaagggtc cggatcgcat
    16141 tatttattcg atcactgaaa aagggaagaa aaaactgacc gactggttat cattacccaa
    16201 cgaaaaagaa tatgtgcgat atgaaattct cctaaaatta ttttttggaa atctcagcgg
    16261 tccgacaaat acaattcaaa gaatcgagac atttcaaaaa cagcatcagg aagatttaca
    16321 gctgatgcag tcatttaaga cgaatttgga aagcgtcttt caggaaaatg cagatcatct
    16381 ttattattac ctgacagtct tgttcggaga acatatttat aaagcttatc tcgattgggc
    16441 agatgaagcc ttggcattta taaataaaaa tctgctatcg gaagaaaata agaaagagag
    16501 ctcatgatga agcgccaaaa aattcgtaaa ttcctgcttt tattttcatt cttattattt
    16561 cctgtcacct tgtattattt ctctcccgta ctgattatac aaggaagctt ttcgggcatt
    16621 gttacgggaa gtttgattct gtttgcgatc cagtttgtca tttctctttt ttggggaaga
    16681 gctttttgcg gttgggcttg cccgggcggg agccttcagg aatattgctc acaggttatt
    16741 gataaaaagg taagcgataa aaaaacagac cgaataaaat ggattatctg gatgccttgg
    16801 atatttacga taatttttgg ttttatctca caaggcggaa taaagagcgt aaatttcttc
    16861 tacatgactg atcacggaat ttcggtctcg ggcataacgg aattggttgt ctatcttttc
    16921 tttgttttct tgattaccgt tctggcgctt acggttggta agcgcgggat gtgccattcg
    16981 atttgctgga tggctccgtt catgatcctc ggaagcagaa tcaaaaatcg gtttggatat
    17041 ccatctcttc atttggaagc gaattcggaa aagtgcgtga aatgcggagt ttgttctaag
    17101 aattgtccga tgggcttgaa tgtcgaagaa atggtccgga caaatacttt gcaaaatgat
    17161 gaatgcatcc tttgtggcgg gtgtgttgat ctgtgtgcta aaaatgtttt gagattgact
    17221 ttcaaaaaag cggagaaacc gaacactaag aaagcatata tcttggatac tacggaagat
    17281 ccgtccaaaa ccgtttcaat atagttttct ttcaggcatt ctgatgatag gacatttagg
    17341 tttttcctac gtaggtttgg tttatttatt gatgctttgg attccgaatg caatatggag
    17401 taagaataaa ccgatagatt atcgtccgga ccgggaaaac agagttttat tgatatttga
    17461 acgtgccgga caagtgtgct gtacttgctg cgttcttatt tttactgatt tgaatattac
    17521 tccgttttcc ggcagaagtt catggttgat cgcatcgttt ttctccatga ttttgtatga
    17581 acttaattgg atacgatatt ttttgaatga acatacggaa aagaattttt atcgtagttt
    17641 ttgtggaatt cccgttcccg ctgcgagttt acccgtttta gctttcttgt tgttaggtat
    17701 ctacggaaat gttatttggc tgattttgtc gagcatttta ctcgggatcg gacatatcgg
    17761 aatacacatt caacatttaa gggcaataaa gaaagtcttg taagaatcga ttttcccggt
    17821 caagcgagtt actatttctt taccattatc cctgctctga aaagatttac cggaaatttt
    17881 tacggatgct tttctttccg ataaatgatc ctcaatgagt tattaccgat ctattaccga
    17941 aagacgaaag acgctgatcg atcatttttc ccgagtttta cagttggaaa aataaaaaac
    18001 gctctgaata cgccgaaaat acggggaaaa agagcgttga tttcatttgg aaaaagtata
    18061 tctacaagcg tatattatct cggagtaatc cggaagccga gaccttttta ttgaactgat
    18121 tatcgtgttc gacggatgaa atcattaaaa accgaaggca tctttcaaga cgaaaaaatc
    18181 aaatcgaaac ggaattacca accggaagat ttaataccca gtccattatc aatctgaatg
    18241 atctggccga ccgtaagatc gagaatatat aatgtcgacg gaggatataa tccgccggca
    18301 ccgggttcct ttctgatata agccagctta gtctggtccg gtgaccatcc taataaatca
    18361 tccgcataat caataaacag tttattttgt aaatcttttg ttgaagcgat gaatagttgt
    18421 cttgagtcag cttcctcaga aataacgaaa tattcaccat cgtattcgga atatttaaag
    18481 cggatatagg caacgtaatt atggtccgga gagagaatcc ttcccgggat catataatta
    18541 ttttcttcct caatgggaaa ttgtatatcg tagtccgggt aaattttgag atagtcggat
    18601 tcctgaatga cgtcacccgt ctcgatattc atataatacc gttcatccga tatgccaatg
    18661 gagcaggctg cgtttatcat tgagatatat ttcccatcat tactgatttt gtctccgatg
    18721 ctgtatgcgt ggctggcatt acttaatttt gcaagatttg ttaatgtttc cgattctccg
    18781 gttttcggat caatacgaac caattccaca tgatcgggac cgataatttc gttatcagtg
    18841 aaaatgcaat cgccgatcgt tcttgtgaag tacaggtaat tctgatcatc ccagccgaca
    18901 agcgtatccg catagatatc cggtttggaa tactttttcc aggaataaag aatatttccc
    18961 tgaagatcga ggatcataaa tgaatcactt aattcgactc cgacatattt tgaatcaggg
    19021 gaaagcatag cgttatagta taaaataccc ccggaaagac cgccatcaaa ggtaagtgcc
    19081 cgggttaccc ccttttcagg atcatagaca taaacatttc cctcttttgt ataaatcaaa
    19141 ggtcggacat cgggtttatt ttccgctaca acaggtgtgt cgtcatttgt cacaacaatg
    19201 ctctcattgt tatccatctt tccgactata aaaatgggaa tattaagaat atagaatttt
    19261 gtcccttcat taaaagagat tatcccttgg gattctgttt tattctcaat atcgaaaaga
    19321 tatatcgaac ttgacagagg cggttttatc gatgacgatg attcctcctt atcatttgac
    19381 caaaaaatgg atatggtctg tccgtcattt tgcaagaatc gataatcaca taaatcatta
    19441 cgctcttttc caagggtatc cataacacgg aatcttccga gaggcattgt gtcgtttatt
    19501 gcaaggtata aagttctaag aacattcagg ctgatgtcgt taattacata gacatcttca
    19561 atatccggag aataggtgta ccagaatact ttttcaaccg gaggcgttga taagagcatt
    19621 gtataggtcc gaataagtcc gattgcctga agcgtgcttt cttcataatg attataagaa
    19681 gcataaagct cagagagcca ataatttgag taaccaactt cggttatcca aatcggatat
    19741 tctttctcga aaatctctat aattgattca ttaaacaacc gaatataatc ggccatattt
    19801 ccggtaacaa tctttttgtc gataatattt tgagtgatca tatccggaaa agcggctccc
    19861 caataaatat gtataccaat cgcatcaaac ggtggcttgt cccaatattt ttctgcattt
    19921 cgcaaaaaat ctgccggatc ataaccattc ttaaaatcag cggaagcaat taatcctcct
    19981 aaaataaccc tatcatcagg atcgtatttt ttaatgattt tatatgcacg atttaacatc
    20041 tcggcgtaaa tatttacttc aacagtctgt gcgtttggac gaactttttt ccaaaactcg
    20101 atacaattca tctcattgcc gatttcccaa taatcaattc cgtatcctaa ttctttaaca
    20161 attttgtcaa ccgcatcttc ccacttttca aaaaaatcat catcattttc ggtaaaacca
    20221 aagccgtaag ttaacaatcc tacgattttc agattttgat ttcttgctgc ttcgatcatt
    20281 cttttttgat tattaagatc gctaatgtta tcaacgataa tttcctctcg aacgtatgat
    20341 ataccggcat tatttaatgt tttatatgct tcaagcattc cttcagaata gttatttctt
    20401 aatgcaattg ccgagcccat cccaaatcca tctgataagc cttccgcaga tgcacatgaa
    20461 aatatcgaaa aaatcaaaaa gatcgggaaa aggtaaataa agatttttgt tttcattatt
    20521 gactccattt tcggacgaaa tcgtgccttg tgacggggaa aaatgaatcc ctttgaaaac
    20581 caggtgtttt gaattttttt atttttcttg cttattattt gtatatgatg tgaattataa
    20641 acaaaaataa aaaaacagtt aataaccgaa tatatcggtg ccgagggaac caagaatccg
    20701 acctggacac gtatgctgat gggtgcctca gagggcgnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20821 nnnnnnnnnn nnnnnnntat cggaataagg gcgccagcaa agcggtgccc ggtttcgacg
    20881 tcattgatta cggacaagag gattatgtat tcggggataa ggacagtgat gcacggcatt
    20941 gggatacttt tgtgaacaag atcttccaaa gcccggaata tgccggcgta ctcgcaaact
    21001 tgttcaacga agcgaaatga agttcacttg gatgaagccg acattttcag atagtctatt
    21061 ttgaaaaata gcagaaagaa taagagtatc gatccaatcc ggaaatgaga aaacaagacc
    21121 gccgagcttg gcaatgataa attgtcggct cggcggaaaa atgtttgttt gtcaaaagaa
    21181 tcacactccc catcctgaaa gataagagaa tgatatgtga ttccgaatta ttttacagat
    21241 tgagcttcgg aattcttaag gaaaggcaac tcagaccgcc gtcctgcttc ataaattcac
    21301 tcatcggtgt ttccaacaca ttaaaaccgg cagcgcgggt ctgtttaatc gtctcatcaa
    21361 aaccttccgg cagaataacc gtatcattca cccgaataca gttcgatgca tacccttcgt
    21421 tatcgggaac gacaattttc ttgaaaggcg ctaattccga ctctttcaga atggcttttt
    21481 gcagaataaa tgtattctct ccaatgtatg acatgcatgt tttcagatgt aatatctccg
    21541 gcagagcacg gatactgatg atgctgctgg taaaaccata cttcttcaga attgcggcaa
    21601 gttgacatgc accttcttca ttcgttcgtt gagataatcc gatgaaaaag tgattttcca
    21661 cttggcaaat atcgcccccc tcaaccgttc ccggtccctc gatgcgttca atatgcgatc
    21721 cgtagatatc tttaataacc ggcaaaattc gatcgacttc tccttttcgt gtcggggcat
    21781 gaaaatttgt taagatggcg acccgatcgg tgacgattac cgggtcttca acgaaacaag
    21841 aatccggaaa atcgttatcc ggctctaaga cgattacttc gagaccgcat tgacgtaaag
    21901 cttggatata ttttttatgt tgtattttgg ctaattccgg atccgctttt ccgaataatc
    21961 cgggtgtaat tccttctgcg aaggaatcgg ccggagttcg gacaaccgct ttttcaaatt
    22021 tatacataat ccctttcctt ggcattcaaa aaatttaaaa gatttttctg ttctttggtc
    22081 gaacacgaaa cgttactatc ataccagatt atcgaaaacc aaatgatgcg ataaaaaaaa
    22141 tcaaaaaagc ccggattaat gggctttttt gattcctaac ttgcctggtt gctttataaa
    22201 attttgctca ggaataactt tgtccgttct tccttgggat tcgtaaataa ttcgtctttt
    22261 ggagcttctt ccacaatttt tccctcatcc atgaggatgc agcgatccgc tgccgcacgg
    22321 gcaaaaccca tttcatgaga tacgacgacc atcgtcatgc cttctttcgc caactgaatc
    22381 atgacttcca gtacctcctg aatcatttcg ggatccaatg cgcttgtcgg ttcatcaaac
    22441 aacataattt tcgggttcat ggctaaagcg cgagcaatcg ccacccgttg ttgttgtccg
    22501 ccggataact gaagcgggta tgctgaagct ttgtccggaa ttcccacttt ttccaaaaga
    22561 agcatggctg tttcgtaagc ttccttttga ctgcgtttac gaacaatctt ttgtgccaac
    22621 ataacattat ccaatacgga taagtgaggg aacagattaa actgttggaa taccattccg
    22681 acttccatcc gcaatttatt aatatcttta tgatctttta cggataagcc gtcgacgata
    22741 atttcaccga aatcgatatc ttcaagatta ttaatgctgc gtaataaggt cgattttccg
    22801 gaaccggacg gtccgataat cattacgact tctcctttat aaacatccag agaaacgcct
    22861 cgtaaggctt ggatatcgtc tttataatat ttataaacat ttctaatgct gataatgggt
    22921 tcttccattt tatcttcccc ccattttcga cttgttttct aatatcttca ctaagcgggc
    22981 tgaaataagt gtcatcagca gataaagtaa cgcgaccatc agccacgttt cgatcggcat
    23041 aaaagtggtg gacataaatt cacggccgcg tcgggtgagg tctgccaccg ctacaaccga
    23101 caccaaagaa gagtctttta acagagaaat aaattcatta ccgaccgccg gcatgatcgt
    23161 tcgatatgcc tgtgggagga tgatatgttg catcgcctgt gattttgtca ttccgagaga
    23221 acgggcggct tccatctggc cggcaggtaa agattcgatt ccggcgcgga cgatttcggc
    23281 catataggcc gcgtaacaaa tggaaatacc gataattgcc atagcgaccg gatttgccag
    23341 ataattttca aacggttcgt aatgcattga ttttccgata ttgataacaa ctgcgggaaa
    23401 tgcaaaatac caaaccaata actggactaa taacggaatt ccgcgaataa tttccacata
    23461 aaccgatacc agcccataaa ttatcttatt ggtagagacg cgtcccaagc cgcagaaaag
    23521 tcccaaaacc atggtaaata tgaacgagaa aacagttacc tctaaggtga caataattcc
    23581 atccgagaca taatcgagga ttcgggaaaa gggatccggt ttcagtacca ataaaagaat
    23641 aatggaaatt aagacaatca gtactaatcc ccaccatgcg tcaatcttcg ataattcacc
    23701 tacttggggt aaatctttat caatccattg attggtatca ttttcgtttt tctttttact
    23761 caacataaac ccattccttg atttgttccc aactaagaat tattgcattc caaatcggtt
    23821 gtttttaatc ttccggaaga ttttggttat tctatcacag gaatttcgat gggggatgga
    23881 aggtatgacg gatagaaaat aaatcattat attaaaagat cgaatagaat ccgttcgaaa
    23941 tcttccgcga tttttttacg ggaaaaatgt tcccgaatcg tccggatgcc gttttgcccc
    24001 atgcgtctac agtcttccgg attgccggca agtaaaagaa ttgcctcttt cagagcttgg
    24061 ggatctcccg gcttgacaaa gagaccggca tcgtattgct cgaccaaatc tcggataacg
    24121 ccatcgattt ggcaaagcac cggcttaccg gccgccataa agtcaaagac cttattgggg
    24181 taggtagtgg taaaaagcgg aatggctttt aaaaccgcta aaccggcatc ggcagcggcg
    24241 atgacatccg cgatcttttc cttggggacg ggaggaacga acaatacatt attcaaccct
    24301 ttttcttctt tttgtttgat cagaagatcc ttttctttcc cgctgccgac taaaacgatg
    24361 cgaatccgat catcctctcg aagcaattcc gccgctttca ataccgtgaa tagatcgttg
    24421 gcgggtccgt gtgctcccgt atacattacg acgaatttct cttcaagttg atgttctcgt
    24481 cggaacgaaa agccgccatc ggtcgaaaca aacagatctg gatcagcgcc gttaggaacc
    24541 agaagggctg tttttccgct gattttttga atatggggaa taaatccggg tgaattgacg
    24601 atgatccggt cagcatgagc gtataaaaac ttttcaagcc aaagcgaagc ttgaataagg
    24661 aatctgttct ttaatacacc cattccgacg gcaaaatcgg gccataaatc tcgaatttcc
    24721 aacagaaatt tctttttctg ttgtttcgca attacccacg cggcaaatgc ctgaaacaga
    24781 ttgggagaag ttccccaaac gatatcaata ttttgcacct tttttgcggt ctttatcgat
    24841 gaaaacatga aactgaagaa agacattacc cgcgggaaaa aacctttatg taatcctccg
    24901 cttgtatagc aataatagac atcaattccg tctatccggt cgattggctt cttttggata
    24961 tccggctggt tttctcctgt taaatagctg acgggactcg tgattacggt cacctgatga
    25021 cctttttgaa tcaaccagtg agccaattcc gcatggcggg ttcctcccgc ctctttaacc
    25081 gtcacgaatg cttgatgaat caaaaggata tgcatacagt cataaccccg gtggtaagaa
    25141 tttccaacgc gagcagataa cgaaaggggc agcggtctcc gcggttaaaa tcagagaaag
    25201 ggcgggaata tgaattccgt aggtgggaga gtaccaaccg cagatgtcac gaaacggatc
    25261 ggaattttcg gcggaagaac cggtcggaaa tatttgttga cccgctcgaa taattattaa
    25321 attcagttga attgtattgg cggaaaaaat tatttttaca tctttcgaaa gaaaacacca
    25381 atggttattc tctttcagaa cctcgaattt ttgttccggc agcagccaat gaaggcggaa
    25441 cagatgtttc ttttccgaaa gatttccatg aaacaaagtg tttggggctc gtggcgaatt
    25501 cggaactcgg atcggtaaga ccttatccac cgccaaccaa tcttgtcgag ttttttcgag
    25561 aattcgtgaa tgttttaatc ccaaccgttt atagccgctg tgctcggcga tttttcgaaa
    25621 ttccgtttga tccagaatcc gtgcctgatc ccaatccagc cataaaaatt ttccggcgtc
    25681 gatcatcggt tcctgacaat caatcgtcag acaattatga acgaacgcgg attttaatcg
    25741 attattccat ggcggagcag cggtataacg gtaggtaccg gcatctaaag ccatacattt
    25801 accgcccttc caaagagaaa catgcaattg atccgcatga gcaggtcggt ctttgaaacc
    25861 ggctgcatgc agaattcccc attgattccc tgcagaaatc ctttgaacgt tttgtgaata
    25921 aaaagtggat tcgaattgag aatccggatc gacgcccgcg cgattataac aaccgagcca
    25981 aattcgcatt tcatcccacg gtccgggcgg gaaaagaggt tttccgagaa agacgatggc
    26041 ggcggtttgc agggtggggc gaaagtcgtt atagtttccg cccagagcaa aaagcagaga
    26101 tccgtcattg tggccggtat tacaagcttg gccgctgaat tcatccgtgt gttttgccag
    26161 ccaaaggacc gatcgacgaa tcttccgaat caaatttgcg ggccaagcaa ttttttgggt
    26221 cttcaacaat gaatccaccc aaagaacaag ctgcagcatt aaccggtgat aattgacact
    26281 gtattgaatg taggcaccgt cgggaaggat ttgagaattc agtgcatttt gaaaatcttt
    26341 ccatccggtc gagaaccatt ttgaagcatc cggtgatttc ggaagaacgc aagcggcggt
    26401 catcaaaccg gctgcctcac ttaatcgatg gttgttattt tgtgcctttg cataactgcg
    26461 cgtttgcatg atccgcgttg cgtgtaaagt aataaaagcc ggtaaatccg ggaattttga
    26521 atttttgtcc ggaagcgagg gctgtaaaat acccgccgcg aaagtgaccg aaatcatgcg
    26581 gatagctgct tcctgggctg atacccaatt ctcaccgtaa tacggaggat tcgattcaat
    26641 gaaagtctga acctgtcgat aaaaaaattc agggtaaatt cgatcacctg tcaagacata
    26701 agctctgcaa agtgcggtca cccaacaaaa tcttgccggt tcccaaatat ccttgatatc
    26761 taaattttct cgagacggta tccgggtcca aaaaagatcc ttatgcggag gaaccagatc
    26821 caaagaccga atgatattgc cggaatacgg cctgtagcct accgtgataa tttcgcctgc
    26881 agattccaga atgcttttcg aatctgcaag aagaaaaggc aggatttcat cttttcgacc
    26941 gttcggaaga ggagagaaaa caacggtttt tttgttattt ttactgtttt tcggaataga
    27001 ttgcttttga aaaaaaccgc ttttcacctt taatgtatat gttccgtaat accagagacg
    27061 ttttattcca aatgaccgga gcgcttttaa tttttttgag atcggattca tcatttggtt
    27121 ttgtcttatc aatcagcttc aaactgtttc ataatcagag aaagatattc agatcgggaa
    27181 ctgccacaat ctcattgctg tgcaacgaat ccactgcggc gagcgaagtc aatgatgaat
    27241 aaaagagctg ttccgtggga atcggttcat gctccccgtt tatgattgcc gagacaaaag
    27301 cctgccagga agctgcatgt cctttatcga ttcgcagcgg tgaattggtg cgatgatggt
    27361 tcccgcgatc ccaagtttcg agcgatttaa aatcatttag gatcgcgatt tttccgccgc
    27421 caaagacctc caaacgttct ttcgcatagc ttttatcgcc gttcgccaga tatgccacgg
    27481 tcccgataga accgttctca aactttactt ggatgctgac attatcatcc tgataacgtc
    27541 cgttattggg caaagcaatc gtattgacct gcagcggtcg tgattgggtt aaaaagagca
    27601 taaagtcaag catatggcag cattcgccga gaatccgacc tccgcctatt tctatatcct
    27661 gtgtccaatg agtgagcggg ataaacccgg cattgaccgt atagtgcatg gcaagaggtt
    27721 ccgcgcaatc agccaagaaa ttttttaatt taacagccaa cggggcaaac cgacgattgt
    27781 aacctaccat caaaatcggc ttcgggttgg cttttttggc ttgataaaca gcctgaaagg
    27841 aatcaaaatc aagggccagc ggtttttcac aatagacgtt tttttgacgg ttaagcgctt
    27901 cgatgaccat ggaactatga agattgtgac gtgtcagaat gacaaccgtg tttatgtcgg
    27961 gatcattaag gacagtttcg gcattgacac ccgctttttc gaatccgaat ttctcggctg
    28021 cctcacgggc gctggcacct cgattggaaa cgatagtgac aagccggatc gatttccccg
    28081 cgcttttcaa aatcggaaga aaagtggcat tggcataatt accggcaccg atgacgccca
    28141 gattaacggg ttgaggaaga gacggttccc gcagttgaac agtatttttt gtaaaaattg
    28201 tctcatcatt ttggaacgga tattcaatca gaatcccgag ataaggttca tttgtctttc
    28261 ctttaatgat ctcatatgct ttttcgcctt ccgcaatcgg gattcgatgg gagatcagcg
    28321 gggctgtttt gacttttcct tccgctaaca ggtcgataaa tgcttccata tttcgacctt
    28381 ccgtccagcg tacataaccg atgggataat cctttccttt ttcttcgtat tcgggatcgt
    28441 atcgtcccgg cccgtacgat cttgatactc taaaagcgat ttctttgtta taaaaaatct
    28501 tgcggggaat attcaacccc acatcgccga tcgcgatgac atttccccga tctcgacatt
    28561 cgtttcccgc cagttcgatg gtgtcattgt catgattacc cgcacagatg aatacatgat
    28621 caaatcccct tcctttcgta aacggaagag caaccgaatc tatttcatca cgcaaacaag
    28681 catcacagcc aagatcctga gcgagattta cgcgttgaga ggaaatatca attcctaaaa
    28741 ctcggcatcc cgccgctgcg ctgatctgta tcgtcaataa accgagtagt cccagaccga
    28801 tgaccagaac agtttcatta atttggggaa ccgccaaacg gatcccatgc atggcaaccg
    28861 atccgagagt cgtgaaagct gcctcttcaa aagggacgtg atccggaatt tttaccacaa
    28921 gattcttcgg aacagtgcaa tattcggcat gaattgcata attcccgccc ccgcaagcca
    28981 cccgatcacc gatccgaaag cctgtgactc cgttgccgat tgcgataatc tttccggcgg
    29041 atgaataacc ggggtacatc gggatatcta accggttcaa agcggattca agcgaggaga
    29101 cgatcccttc ccgcttagct ttttcgatta cttgtttggt taaatccgga cgcgaagccg
    29161 ccttttcaag caggtttttt tcggcgaaat ccgccgaggt ccgttcggtt ccggcggaaa
    29221 ctaaggaata ggcattttga atcagaataa acccgctctc cggttcggga accggaattt
    29281 ctttgatggc gattttaccg tttttcatgt tctgctgcag ctgtttcatt ctttgctctt
    29341 accccattcc aaaggattct cgtgaaagaa ttatacatta ctctcattat tccggaattt
    29401 gcgattggat cttcgtttta aacggttaaa taagagaaat tatgatgaat gccgaacagt
    29461 tatacgccgg aagtcattca cgcgatatca taaaatcctt ttataatagg attgcaatga
    29521 aaccatttac ggagcatttt ataaagagtt ggcatattac agcggtgctg atgatttcat
    29581 gcggtcttgc cgcttttgta atcgcccatt ttcttcccgc aaggtacact gccaccgcct
    29641 ttctctcttt ttcaatcgat cataatcaga ccggatcgtt gacggatctg gaagaagatc
    29701 ggatgatcgg aattaccgaa gatatcatca aatcggatga agtttttgcg gaggtatgcc
    29761 gaaatatgga aggatgcacc aaagaaagct tcctgaatcg cattcaattg gatcgtacca
    29821 ataaccaatg gtatatgagt gtttccggag aggatccgca agaatcggct gccatggcat
    29881 cggtttggtt agacgaggct tataccgtac tgcaacgctg cctgagagct gcgttggagg
    29941 aacggggatt gaaggaagtt ttgaataatt tggtgctctg tttcgaaaac tctgtttccg
    30001 gttctttgac aaatatctgc ccgtctgata cggcaatact gttcaagaaa atcgaagaaa
    30061 tatccgcgga gattatggaa aagcagcgtc ttgcgaaagg aatttctccg gcaattgtat
    30121 tgggcgaaaa aagaaatgtt ccgggagata ttccggttgt gcgctcgaat gcgacaacgg
    30181 gttttctgac attgctcggt ttgttgatcg gttttttaat ctctttgctt ctgtgtttga
    30241 tgaagccggg agtttcgaaa gagccaaaaa ccgactgcga tccggcggaa tgaaggcgaa
    30301 tgaatccgat atcgtttttt gaaaaacaag ccgattcccc gatttggaag ggaatatggt
    30361 ttcttttctt ttttttgctg ccgatcactt ccatgccggc agtggttaat ttggttcatt
    30421 ccgatgtggt ggctgcgcct tccggattga ttctggcggt tttgttgatc ggatgggtat
    30481 ttcctaaatt gctttcggga gagaaatttt ccgaaaatgc gattcctttg ttttgttttg
    30541 tgattgtcag tttgatatcc accgctgttt cttttttcta cgaaattccc atatataaac
    30601 aatcggatcc tttaagatct tcaatttcag cgatcattac ggtatttatc ggagctgggt
    30661 tttttattgc ggtgatgttg tgggtccagg acccacaacg attgaaactg gctatgcagg
    30721 cgctgaattg gagcggtgca ttggttttga tttggacact gatgcaagct tttttttggt
    30781 atacgacatt cggttatccg caatggatga aagacattca atatgccgtc tcggtcggtt
    30841 cgctgtaccg ccagcggttt gtaggtttcg cgctggaacc ttcatggtta gctcatcaat
    30901 taaatctgtt ataccttcct ttttggtttg ccgctgccgt taccggctat tccgctcatt
    30961 ccaaacggtt cgggaaaatt acctttgaac ggatattgtt tgttttgggt gcggttgtcc
    31021 tttatctgac gctttcacga gtggggttgg ctgcgtttat tctctcaatt ttgttttatt
    31081 ttatatggcg gatcgtccat tttgcggaga cacaatctgc cggtacaaaa gctccgaaga
    31141 aaaagaaagg gctgatcctg ctcggctttt ttgttttaat tccctttatt ctgcttttta
    31201 tcctctggac gcttacaaaa ctcgattttc gtatggcgga attatttacc gtcaacataa
    31261 aaggccgaaa taatccgctg atgtatttgg cggaaaaact gagtttggcg tccagatttt
    31321 cctattggga aggcgggttg aagatatttt ctcaattccc gattctcgga gtagggctcg
    31381 ggcatgctgg attttatctt ccgcagaatt tgagcgcttt tgctttacgt ttggttgaag
    31441 tcagggattt actgtttcga tccgaagttc tcttaaatat taaaagttta tggatccgaa
    31501 tcttggcgga aaacggaata atcggctttg ccttttttgt cgcatggttg gtgtccactt
    31561 ggggattgag ccggcggatg atgattcgaa gcagttctct gatcaaaacc atcggttgga
    31621 tgggttgttt ttctgccgtt gctttcttgc tggaaggatt cagtttggat acatttgctt
    31681 tgccctattt atgggtttct atggggttgg tcggaggtgt ttccttgatg ccggtcccga
    31741 acggaagctc cgagaagtga aagatttgaa agagctgcat atttcatgaa aaaattaatg
    31801 attttaattt ttttatcaac tcgttttttt ctgttattgt cacctgttca agcggaagaa
    31861 tctgaattca atgcagcttc tgccgaagag atgcaggagc gcttggaatc aattcaaatc
    31921 gatccagcgg acagagaaga gattgcaagg cgatttaatc acttaaccgg ggaactctct
    31981 ccgattccgg aaccgttgaa ttggcagatt ggaatggatc accttttttg ggtaattaat
    32041 accgatcgta aaattactgt tcaaatcgat gcggaagccg tttatgtggg agaacatatc
    32101 gtcatatggg tagaaaaaac ggcactttct caactttccg acgcagtcta tgatcaattc
    32161 aagaaatttg actttgaaat ttatcctttt gaaaaatacg tgttcgggtc ggagctttct
    32221 cccgggattg atcatgattt gcggattcat gctcttttca ccaaaaaagc cggagccgga
    32281 atccttggtt atttttcttc ccgggatgaa gatcatcccc aggtttctcc tcattccaac
    32341 gcgatggaac tctttatatt ggactcgacg atgctggcgg gaaaccctga ccagattacc
    32401 aatacgctgt cgcatgaatt tcagcacatg atccattatg cgcatgacgc caatgaaggg
    32461 tccaatattg acgaaggatt ttcgggattg gccgaatatc tgattcataa tcgattatcc
    32521 gaagcctatg agcgatcttt tttggaaaat ccggatattt ccctgacgtt atggccggtt
    32581 tccggttcaa gtatcccgta ttatggcgct tcttttcttt tttcgaagta ccttgcagat
    32641 cgactggggc aggattttct gaataaagta gtagaacagc cgcgaaacgg ttttgaggga
    32701 atcgatgctg cgttgaaaga agtgaagagc acttttactg ccgatcaact ttttgcggaa
    32761 tggaccgcag ccaatctttt gtatggtttg ggtatctcaa acggagaatt ttcttaccgg
    32821 gattatgaac ccccgttacc gaaaaacggt tctatgataa agagtttgtc ttgtaaaaac
    32881 caatcttttg attcgaccgc gatgcaatac gggacggatt attacaatct tccatgcgat
    32941 gcggggacct atgaaataga aattaccggc caacctacca tcccgatttt atcgatgaat
    33001 cctattcaag gtcagtatgc atggtggagt aattcagtca acaactcaga tacatggatg
    33061 caacacaatt ttgatttatc aaatgcaaaa ggttcgattc gctttgaatt tgatctaaat
    33121 tatgacttgg aagaggctta tgattttctc tatctttctc tgtctgccga tggcggaaaa
    33181 acttggcaga tgttgaaaac agcccatggg accgacttaa acgaatccgg attcaacctc
    33241 ggttggggat ggacggggaa aagcggcgga tggtttcatg aatccgtcaa tctcagtgaa
    33301 tattccggga aaaatgtctt tctgcgcttt gatctgatta cggatcaggc attgaccggc
    33361 gaaggggtgc tgctggataa tttcagaatt gatgcgattg atttttcgga tgatgttgaa
    33421 aaaggaagtt ccggttggga agcaaacggc ttcctgccga tcatgaatca gcttccccag
    33481 cagtattccg atgttcttat caccggcgat tcgaaaaaac ctgcgatttt ctttcataca
    33541 acggaaggct ccgaaacatt ttcatggaca tgtgatttcg agcagcaaga taaaccaatt
    33601 tgtgcgttcg gtatcagtcc gatcaatcga atatctcaat tatcagccgc atatcgaata
    33661 cagataaaaa gaaacggacc atgaatcgtt tcaaaaacag atcatgatcc gttatttt
//
LOCUS       DAOSMM010000019        32964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1377_length_32964_cov_20.098879, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000019 DAOSMM010000000
VERSION     DAOSMM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 32964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32964
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1377_length_32964_cov_20.098879"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK898_04550"
     CDS             <1..728
                     /locus_tag="PK898_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU19337.1"
                     /translation="SLSQKLDSSEQTRTKLVSYLTHELGRPLGGLSSAVDALQMGAAQ
                     DEKLVQDLTVGMKNEIKRLELLVGDIALLREKTDPINLYVMKPVPLSAWLNEVYSYWA
                     EYARDKDIKLVDEIDQNLPTIKMDENRMYQAVGNLLSNAIKYSPNGKTVTFRAFQTDD
                     EIQIQVTDQGCGIDREDLPHIFESFYRGSENKRIIQGMGLGLTISQDVVQAHRGTITV
                     ESEKGVGSTFTIHLPLKNISPEK"
     gene            730..1425
                     /locus_tag="PK898_04555"
     CDS             730..1425
                     /locus_tag="PK898_04555"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOU19338.1"
                     /translation="MNRTGLSIRQQLVSMADDHYKIFSCKLIPNIPPERILGVRLPDL
                     RKLAQRIAGEDPESFFADAGDETFEEKMLQGMVICYFDGPFNEKLALVEKFIYKIDNW
                     SICDSFCAGLKLPLKYPGEMWDFITPYLQDQREYFIRFGSVMLLSYYVDETHLHAALS
                     LLDQVRSRDYYARTGVAWAISEYFSHFPEETMRYLHESSLDDQTFNKVLQKIIESRFT
                     DAKMREEIHALQR"
     gene            1599..>2757
                     /locus_tag="PK898_04560"
     CDS             1599..>2757
                     /locus_tag="PK898_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU19339.1"
                     /translation="MKSTTRNTILSILLTLLIVFSAFSPVLAQSQTAEQTISETSTSF
                     SKLYEEVNPSVVYISVAIQGGSSNTQQFQGLPDDLEELLPFLKQFYGPQGQNNQDQES
                     EPNQNQNQNQNQNQNQNITYGSGTGFVWDTEGHVVTNNHVIEDATDITVTYYDGIERK
                     ATVIGADPDSDLAVLSVEDYRKDIAPVKMGDSDTVKTGEYVAAIGNPFGNVGTITMGI
                     VSALGRNFPIDDGSTGSGGHYTIPDMIQTDAAINPGNSGGVLINLNGEVIGVVNSFVS
                     ATYSSAGIGYAIPSNLAVRVIPKLISDGKYEHAWIGISGIALNPDINAKLELDRDQRG
                     ALVETVQSGSPADKAGIKAGDETTTINGAEITIGGDIITKINDREVKSMDDI"
     assembly_gap    2758..2857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2858..3379
                     /locus_tag="PK898_04565"
     CDS             <2858..3379
                     /locus_tag="PK898_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOU19340.1"
                     /translation="GDIITKINDREVKSMDDIISYLAFHTEVGDKVTLHILRDGKEQD
                     IELTLAARPSVEERTGVKANENTIREQVGEAWIGTYVKDISANDIKKLELPEGTTGAL
                     ITQVTKDSPADDANLQENDIIQKIDDTEIKSVDDLKAELSKYMPGERVTLTILRDGKT
                     KEVRLTLGTTKNK"
     gene            complement(3493..4662)
                     /locus_tag="PK898_04570"
     CDS             complement(3493..4662)
                     /locus_tag="PK898_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOU19341.1"
                     /translation="MNFQQNIVCPDYNHSNLGIISSVLNYYAVPSSTKSSTDVDSLLA
                     QKRPRNVIFLLIDAMGTGILNRHLPEDSFLRSHRRRDLTAVYPCTTVCVTSSFFSGLQ
                     PVSHAWLAWSLYFKEWDECIEVFPYRDAFTGEPIDPNEKNILDFMGFRTIFEQIENNA
                     DRKIKIDCIFTDVARQRLVQIAGDRLTKAHTLEELFQNIELKCAEEGDHFIIGYWKSP
                     DDLLHNNGLSHESVTEFLSKTDSLLEQYAPRFSDAVGIISADHGMQDIFNHFHLDQIS
                     PLSELMNHYPSGDPRAKAISVIPGKEDLFASRFERELGDEFILLSADEFLRRGFLGEG
                     PLHPKVKDFLGSFIALGVGHSDLLYAPPGAKPPKEFKGHHAGLTEDEMQVPLILF"
     gene            complement(4672..5448)
                     /locus_tag="PK898_04575"
     CDS             complement(4672..5448)
                     /locus_tag="PK898_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOU19342.1"
                     /translation="MPDITILQILILSGFAFLAGLIDSVAGGGGLIQLPAIMAILPYA
                     PVASLLGTNKLSSSCGTGMAVFQYSRKVPIEWKKVLPIAGVAFCSSIFGAMTAVSVPN
                     QWMRPLVLVLLTVVLIYTILNKNLGVRVHEVSFSFRQQLGLSLLAGLVIGFYDGFFGP
                     GTGNFLILALIGIFGMDFLRASASCKIINLATNFGALILFGSQGAIFAYLGLIMAVFN
                     MAGSYVGVRLAVLKGSKFIRILFIVVVSGLLLKQCIDYLL"
     gene            5662..6726
                     /gene="thrC"
                     /locus_tag="PK898_04580"
     CDS             5662..6726
                     /gene="thrC"
                     /locus_tag="PK898_04580"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU19343.1"
                     /translation="MSYQGIIHRYHEFMDLPPAVQPITLYEGNTPLIPVPAIADNLGG
                     GFELYVKYEGLNPTGSFKDRGMTTAMTMAKHNGKKTVICASTGNTAASAAAYASRAGL
                     RAIVMIPEGKVAAGKLAGAVAYGAEIIQIPGSFDDALSFVVEISQKSEIELVNSLNPY
                     RMEGQKTASFEICDELGSAPDWLCLPVGNAGNITAYWMGFKQYNRIKNTGLPHILGVQ
                     AEGAAPLVIGHTVEHPETVATAIRIGNPARGEQALMAAEESKGRIIAVSDEKILAAQK
                     LLASSGIWVEPASAAGLAGLTDRIKNNSFDPKGKRIVLVCTGHGMKDPDIIISRGIKP
                     VRLPVDFDKVAEYILGGGNR"
     gene            6723..7634
                     /gene="thrB"
                     /locus_tag="PK898_04585"
     CDS             6723..7634
                     /gene="thrB"
                     /locus_tag="PK898_04585"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOU19344.1"
                     /translation="MRSFSVYVPASTSNIGPGFDCVGLAVNLWNEARFYFDSSLSSIE
                     ITAEGYQSKVQKQPSENLIFRSFLHFFELKGIKVPEGLRIHCVNRVPIGSGLGSSSTA
                     ILIGLLAADELLGTKTSQQGILEIAASLEGHPDNVTPAIFGGLTAASMDETGVRTERF
                     PIADWNMAVVVPHFNLSTKAARKALPDTVPFRDAVFNLNHAIFLMRALQDGDEEELRF
                     AMQDRLHQQYRMRLIPGSEAILQAGVDAGAAATGLSGAGPGVIAFSMGKTDQILAAML
                     QAAHLAGYEADGFELKTSSNGAWIMPL"
     gene            7649..8284
                     /locus_tag="PK898_04590"
     CDS             7649..8284
                     /locus_tag="PK898_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU19345.1"
                     /translation="MKKKLIIIAIVLICVTGCGKKKIADPIQPLTKVTISPEREALYE
                     ATKEAWLTVNPVEIKSTQSDHATPVPQDQAPKAVESESIVLANTKPAAVTEAPQGFYQ
                     LQAGETPKCIARRFNVDWLKLYSMNNISFENENDIQPGTVLVIPQNSVWNEKHGPRAS
                     LPHPTDYNVQAGDTLNSIACQFGDVTPEQIALENGITSQEQLIPGLSLHIP"
     gene            8297..9403
                     /gene="tgt"
                     /locus_tag="PK898_04595"
     CDS             8297..9403
                     /gene="tgt"
                     /locus_tag="PK898_04595"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU19346.1"
                     /translation="MTEPETEKSWLETPHGRLDYPVYLPDATYGMVRGVDAVDLEQAG
                     VQGLVMNTFHLMQKPGSLTVQSLGGIHTMSGWSKPIITDSGGFQAYSLIRENAKFGKI
                     NENGITITPEGSKRKLLLSPEKTVQLQMSYGSDVVICLDQCTHPDDSDQVQRESVRRT
                     IEWAKRCKHEFTKRLIHTEKDTKPPKIFGVVQGGNSFELRKTCADALLEIGFDGFGFG
                     GWPLDGEGNLVEDILQYTRELIPAEYPLHALGIGHPENVVRTFRLGYDIFDCAMPTRD
                     ARHGRIYAFREDPPQLEGKWLRYLYIDDDKYIRSSQPLSPGCDCLTCTRYSTGYLHHL
                     FKMNDSLYPRLATIHNIRILTRLTELLRREKYGK"
     gene            9393..10208
                     /locus_tag="PK898_04600"
     CDS             9393..10208
                     /locus_tag="PK898_04600"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19347.1"
                     /translation="MENDSSVLQIAAEISAGKNFREIDPGLILHLVKTEMQPNESEKE
                     TVKRVRSKLHQISNAYREGVSNYQDLITAIWQSDNSLTSLEAAKPLAIELMKSHASTR
                     ERIAILDVFYSQIFAALPPIQSVLDLACGLNPLAIPWMKLADHFTYEAYDIYRDIALL
                     LNVFFEKLNIRGKADSMNLLEEFPQSECDLTFLLKTIPCLEQVDKNAGTHILNQIRSP
                     YAVVSFPIFSLSGRQKGMKVNYEKHFLEIYDPQRWELERMLFPNELVFLMRRK"
     gene            10205..11029
                     /locus_tag="PK898_04605"
     CDS             10205..11029
                     /locus_tag="PK898_04605"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19348.1"
                     /translation="MNNRKETDDTVDQIVRKILSSPKYRELGIPKESVEDIVKREVSR
                     QTDIKVAEKKAREKLHQVVAPYLGDPDYRSAGTALTQIHRDDREGLKAWCLEMLRSHS
                     STRERLPNLELFYQTVFEFTGKPNTVLDLACGMNPFAFPWMGLEPSVRYYAFDLHTPR
                     VNLINRFFDAWGLQPLATVTDVLVQVPQTPADVAFFFKEAHRFESRRKGSNRAFFQQL
                     SVKWIVVTLPAENLTGRHQMRDRERMLIEKTITGLDWEISETEVGTEMIFFIRKYE"
     gene            11022..12518
                     /locus_tag="PK898_04610"
     CDS             11022..12518
                     /locus_tag="PK898_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19349.1"
                     /translation="MSKKKNSERSRNISPRKEAFDTAWEKFEPLIPEEELPALKACIQ
                     FTPDPAIRLNPQKASISDIGKLRERYHWDTEPVAFCPDGYKLKDTHGIAPGQTNEHRC
                     GAFYIQDSASMLPVTLFDRQKILNKPLILDMAASPGGKTTHLSAASRDSGLILANDSS
                     ASRITILKKVMKNWGSIHHLITVFPGEFFGQWYPNTFDLVLLDAPCSMQSLVSIASHP
                     MRPISEREENALAQRQLSLLDSALKAVKPGGQVVYSTCTLSPTEDESVIDGILKKYGA
                     QITLLDAQQKLPYPAPGLVSVEGSEPNPSLERTIRLWPHRYQTTGFFAALIQKNDTFG
                     FSEQIEKPFRRSREELRFIEIDPRQDHHPIERMNEIFGFDLTEFAEQRECCFRMRGNE
                     LWAVPTVFFDESVDWPPCKSCGMRVAAQTASGWLPDFDWVSLVFDRISQNRYPLDETL
                     AEAWVRGEDLPISLPEHSKGTILFMTDSWNVFIGCGYVSGNRIRNLTK"
     gene            complement(12577..13677)
                     /locus_tag="PK898_04615"
     CDS             complement(12577..13677)
                     /locus_tag="PK898_04615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU19350.1"
                     /translation="MRIQSGMPERKKQTPSWLPYALWAVALVCVFFMGVVMEQRTPYI
                     RGMADDNNVSENVGAQLVNEVENNTDLPDQLNPITVQSSDSVERKAKLTTDKPDRGNL
                     EPQKYTIQAGDSIYGIAKKFGLKPETILWSNYNVLHDDAHNISVGDELIIPPGDGIYY
                     QWKENDDLQKIADKYRVQVEDILSYPANHLDMTNPEIKAGDFILLPGGYRETAVISPI
                     VFEYSKNSGVKKQISGPGGCAWDYVAYGTGTFVWPSASHSVSGNDFWSGHMGLDIASV
                     EGGPIYATDSGTVVYAGPIGGGYGIMVMIDHGNGYQSLYGHLSAVNVSCGQAVSQGQV
                     IGLGGSTGNSTGPHLHFEVRYGGGYVNPWNVL"
     gene            complement(13674..14948)
                     /gene="rho"
                     /locus_tag="PK898_04620"
     CDS             complement(13674..14948)
                     /gene="rho"
                     /locus_tag="PK898_04620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOU19351.1"
                     /translation="MKNESLTNMTLAELRKIAQDMNIPNAQRLKKDSLVVKIRQVQGE
                     QEGVEVRGGILEILNEGIGFLRADYAIGPEDVYVSQAQLRRYNLRSGDQVIGFVRPPR
                     ESERHYGLLRVESINGQSPEEAKNRKTFESMTPIFPNERFNIEYDPASYATRLIDLIA
                     PIGRGQRGMIVSPPKAGKTTILKQIANAISSSYPDVHLIIALIGERPEEVTDMDRSVD
                     AEVVASTFDEPVTQHVRTSEITLERAKRLVEIGKDVVILLDSLTRLARAYNMVVNPSG
                     RTLSGGMDPSAMYPPKRFFGAARNLEEGGSLTILATALVDTGSRLDDVVYEEFKGTGN
                     MELHLSRKLQERRIFPAIDIEKSGTRREELLLGPDLLPRVWLMRRMYMQMIGQPPQGA
                     GMDSAVATEAIIQRMMKTKTNNEFLENLTEDM"
     gene            complement(15241..15939)
                     /locus_tag="PK898_04625"
     CDS             complement(15241..15939)
                     /locus_tag="PK898_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU19352.1"
                     /translation="MERESVFNFLDHAYKLLTHPSYVNLEFVPKEGPIIVAINHMSRL
                     DFPFLATKGFRDDWSALIADSYQSWPVFKQICDAIDMIWIDRSKADFTAFKKAFDWLK
                     AGKSLVLAPEGTRSRTGQLIKGKPGIVLLAMKSGVPVCTGSITGTEHFMDDFRHFRKP
                     KITMRFSPPYTMKKIDPERRDESMEEETTELMCRIAAMLPEQYRGYYKDNPRIRELTE
                     EWSKIPGLKLPDNI"
     gene            complement(16016..17317)
                     /locus_tag="PK898_04630"
     CDS             complement(16016..17317)
                     /locus_tag="PK898_04630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU19353.1"
                     /translation="MLKKNLGIVILAVLISLIFGTVVFAEDEWVPFEAGQDNVQPMEC
                     TIVKPVGPNEKITAVLPPITDEDWSIGPKDAPLTILEYADFQCPYCSGAGLALLEFQK
                     NHPDEVRYVYRHFPLSFHEKAPMAAYAADAAGAQGFFFEAEHLLYSTQSDWSGLESLD
                     AFETWLKDNFKTQISGLNYDQWLTDFADPDLRAKIDKAFDEVVATGVVSGTPTIFLNF
                     NSYQGSYDEAALTSYLKFFELQGKAFQECPPNLIDVNKDYRAVLDTTKGKITVDLYTE
                     QAPLAVNNFIFLATQGWFDGIPFYKVVPGFIVQSGDPSGSGMGNPGYQFVNEKHDLKF
                     GDPGVVGMANSGKDKNGSQFFITFNLHDYYQQSIKEANDKLTSENKLTDEMIEKEVQD
                     QLNKMSDSYTVFGKVSEGYDVAQTLTTSDSILSVTIEEKAK"
     gene            complement(17455..18072)
                     /gene="tmk"
                     /locus_tag="PK898_04635"
     CDS             complement(17455..18072)
                     /gene="tmk"
                     /locus_tag="PK898_04635"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOU19354.1"
                     /translation="MFITFEGPDGSGKTTQVKRIGRKLIEKGFDIVYTREPGGTEISD
                     QVREILMNMKNKQMCPRTEILLFCAARAQLVEEVIRPALQSGKIVISDRYADSTLAYQ
                     GYGHGFEQETLKQLLNFATGNLWPDLTFLLDIPAEKGLRRRINNQEEWNRMDDYQLAF
                     HERVRNGYHQLAAADPQRWEIIDADQSEEAVENEIMQRLARRLHF"
     gene            complement(18076..19779)
                     /locus_tag="PK898_04640"
     CDS             complement(18076..19779)
                     /locus_tag="PK898_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOU19355.1"
                     /translation="MKQYENNLDDIHALVAIFPEEIGEKIESIGNLSELVEVVFDVGR
                     PPLARYTDDEIQLSEKEVAFEDLETIISQIGDFDLDNRAGMERTLHRISAVRNRKGKI
                     IGLTCRVGRAVFGTLEIIQDFVDSGKSILILGKPGIGKTTMLREAARVLAERKRVVIV
                     DTSNEIGGDGDVPHPAVGKARRLQVAKPALQHEVMIEAVENHNPEVIVIDEIGRELEA
                     QAARTIAERGVQLIGTAHGRTLSNLLMNPTLSDLVGGIESVTLSDEEARRRGTQKTVL
                     ERRSPPTFDVLVEIVERNKVVVYPDVAAAVDALVRGIELAPELRIKTDQGKIIREKAP
                     APVLEGNGFTPAAESDLQIRQKRNILQISGRSKTESGNRRSSRRGVSGESYGMTQSTS
                     PVLTGERDLDSDSQNDPGMKPIRIYPHGVARNRLEQASARLGVLISIVNNVEEADVLM
                     TLRPYYRSRQKMIVDAEERNVPIYVLRSNTINQIEYTLANLFNVTVQQPSGNWENVEK
                     ETTEAIMAVKNGEAVWVDLAPANRMIRQMQHELVEQYGLLSQSYGKEPKRRIRIFQNP
                     E"
     gene            complement(19776..20768)
                     /locus_tag="PK898_04645"
     CDS             complement(19776..20768)
                     /locus_tag="PK898_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19356.1"
                     /translation="MWWFSESLNGGIERNFRIVVAQARTFRLYDMKFFLETENKYYCL
                     SNRTRFTNNQFSLTTCFLDSLNLLSSESIAVSRGPYLTYYWGGQISFDMKKNTAWFSW
                     LIAGKHSNNSNLLELLEFLVAKAGKAGMQSCAVVLDEGQWVTGSFRQIGFSVYSKQTV
                     WCYKGELNSDGENWQSVSVTPFDEYDLFYESQITPLIRQLGSNWNQENIYALKKDGMI
                     QATAKATAQNGNRVYLEPIFNPGVENPAKLMLSLAQQINHTTDQEVYVMIPGFQAWAN
                     DPFNEENFNLVCRQTVFVKNLAAKVAEEKPAIQLENGYLQPVGPANYQMNKKEK"
     gene            complement(20731..21525)
                     /locus_tag="PK898_04650"
     CDS             complement(20731..21525)
                     /locus_tag="PK898_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU19357.1"
                     /translation="MNRIRKILFSFRWMLCIPLGILGLLLIFEQPVFAYQALASPTPD
                     FDGNIFYTVSDLDTCLSISLKMGVDVETIRSLNNLDVNCTLIAGTKILLGRVLTPTPT
                     AGPSPTPTEILPTPTPYDGSAQLCIYLFEDKNGNATAEETETGIAGGAVSVTKRGGTE
                     SFTGLTTGLDLLCFPEVPEGDYNISVAPPKEYNPTTNMNYPITIKAGDTTQVNFGAQK
                     QAAFENSAAATQQQTKKSPLLAIIGIVLILIGVGIVVVFGILKRRD"
     gene            complement(21539..23275)
                     /gene="recN"
                     /locus_tag="PK898_04655"
     CDS             complement(21539..23275)
                     /gene="recN"
                     /locus_tag="PK898_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOU19358.1"
                     /translation="MLTELYIRNFAIIDELELSLKEGLNIFTGETGAGKSIILDAVST
                     VVGGKTDPTFIREGTDRAIVEAVFRPDENREAIREILKREDLPEDSDGDQILLSRDIR
                     AGGRSSARVNGSSVNVNLLREIGRLLVDIHGQSDHLSLLNPSNHIVLLDRFAGNQELL
                     KKYQAEFKKLQKIRTRLTEIRMNDEELLRKKDMLSFQMNEIRSAKINESEEETLKKDR
                     DRLANAENLSQLTQKAMEQLEGRSEEYPGIFELLGYLSKSMDNLSRIDAELAPLGEEV
                     AAINDALNELLASLQRYQETLEFNPARLDAIEERLALLNTLKRKYGGSLRAVLEFAAR
                     AEEQLAGLENISDQTEDLIRQETSVINLLSELAQELSDRRHEASRKVSESVEKELHEL
                     RMESAQFEIRFTQTAAENGLRDRHGRSLAYDENGFDQVEFLIAPNPGEGLKPLAKIAS
                     GGETSRLMLALKNTLAEADSIPSMIFDEIDQGIGGRVGSIVGEKLWLLSRNHQILCIT
                     HLPQLAAYYDAHFHVSKQLSDGRTRTLVKQLQPEESLREMAQLMGGDSPENRRASGAM
                     ILNAQKWKESVK"
     gene            complement(23277..23840)
                     /locus_tag="PK898_04660"
     CDS             complement(23277..23840)
                     /locus_tag="PK898_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19359.1"
                     /translation="MSDEISEIKTCYKHPNRETLICCNQCGRPICMDCAVSTPTGYRC
                     KECVKEQQKKFNTAVSRDYVLSAIVAFLIGALGSFVLYFFNFYSMLTAIILGLLLGNL
                     IAKAVRAVTQKRRAADLNKLTAIAAGIGGAVPILRYILGLFRMIFIGNYAGLMGYTLS
                     VGWGILYIALLCTTILTNMRGFAIRRR"
     gene            complement(23837..24724)
                     /locus_tag="PK898_04665"
     CDS             complement(23837..24724)
                     /locus_tag="PK898_04665"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOU19360.1"
                     /translation="MNQTTKKPERIGVTWHPHLAAAEEEAKKIIEEINTRFHGQAVAE
                     GWSLYDEAFRESLERRPFDLVITFGGDGTMLRTNKLCAPFEVPVCGINMGSFGFMMEI
                     AKNQWREKLPALMNGDWRIEKRMMLHVELVRKNNERTRWEVLNDAVIGHGSKLRPIRI
                     NVRINENYLTDYVADGLIVSTATGSTAYAMAAGGPIMEPELRNILVIPVAPHLCLERG
                     IVLDENDVVEIRSSSEHETIFSPDGQDGMMIDESDTISVSASEFSARYVRLEGKGFFY
                     SNFIQYMQRNPSANRKTTI"
     gene            complement(24731..25249)
                     /locus_tag="PK898_04670"
     CDS             complement(24731..25249)
                     /locus_tag="PK898_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU19361.1"
                     /translation="MNQQLLLGILLLVLALAGLILTERKKASDVLFQGKPVYEDIAPN
                     GKLKAPLFDPETRLAGKPDLLIQKGEQLIPIEIKSQPAPETPPASHVFQLMAYCLLAE
                     KAFAVKPDYGILRYRNKEFKISYTIKRRDELLDRLERIREDDSLEEVHRSHCDSFRCG
                     GCGYRSICDEKL"
     gene            complement(25246..25506)
                     /locus_tag="PK898_04675"
     CDS             complement(25246..25506)
                     /locus_tag="PK898_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19362.1"
                     /translation="MKNKTSQKIITASELGTYVYCARAWWYRNRNAPNENLPAMNEGT
                     RFHRKMGRRLSAMRIARIILILMLISALGLISWWIAANVGAR"
     gene            complement(25755..26399)
                     /locus_tag="PK898_04680"
     CDS             complement(25755..26399)
                     /locus_tag="PK898_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HOU19363.1"
                     /translation="MNYIDKISEIAGRAYQDFEARTLARDNALTKARSLTRHSANAIR
                     AIHRGEADEAHSQLQEGQKLFEALKAELEIYPDLYYAGYTQDAIKEHCEATLTCAFIK
                     NEPIPTAEEMGVLTATYLRGMAETPGELRRRCMDILRMGYSEEAERLLSLMDEIYSIL
                     ITMDYPDAVTNGLRRQTDLLRGIVERTRADLTLSLREEKLKAELQKAIEVFEKK"
     gene            complement(26399..26830)
                     /gene="ssb"
                     /locus_tag="PK898_04685"
     CDS             complement(26399..26830)
                     /gene="ssb"
                     /locus_tag="PK898_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU19364.1"
                     /translation="MFHTIIIAGNIGRDPEMRYTPSGVPVTSFSVASSRQYSNTQGQT
                     VKETIWFRVTCWSKLAEYANNYLKKGNKVLIEGRLVPDPNTGGPRVWTRNDGTASASF
                     EVNASTIRSLTTRSESENAGNASGGVPEYSDSEIDEDEIPF"
     gene            complement(27172..28992)
                     /gene="glmS"
                     /locus_tag="PK898_04690"
     CDS             complement(27172..28992)
                     /gene="glmS"
                     /locus_tag="PK898_04690"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOU19365.1"
                     /translation="MCGIVGYAGSKECLPVLLDGLTRLEYRGYDSAGVAVIEQNQIRT
                     VKAKGRLSCLREKLASMEPLTGCCGIGHTRWATHGEPSDVNSHPHSSARVSIVHNGII
                     ENHAEIRDFLIEKGYEFLSETDTEAAVKLLDYYYDGDPIRTILRTTQELHGYYALAIL
                     FLDFPDRIYAVRRESPLVIGYGDGENFVASDIPAFLKYTRRYSLLDTDDIAVITKDGV
                     DIFNSLGNRVKKEILTADWDIESAEKGGYPHFMLKEIHEQPSALEKTLAPRIREGRPD
                     FSGEGIDDAVFREAQCIHIIGCGTAMHAGIVAKYAMEKLIHLPVTVDIASEFRYADPI
                     LAQGDPVIVISQSGETLDTIAAMRLAKQAGCTTIAIVNVVGSTIAREADKTVYCWAGP
                     EIAVASTKAYSVQLGLLYLLAIKIAAIRGQISPQRELELTAALFSVPALDEKVLSDTD
                     GIKHIASEYMNADHAFFIGRSLDNAVSVEGSLKLKEISYIHSEAYAAGELKHGTISLI
                     QDGVSVVAVATQYALIEKMFGNIREVKTRGARVLLVTQEDITVPEGLADEIIRIPKVD
                     DFFAPLVAIVPLQLFAYYTAVLRGCDVDKPRNLAKSVTVE"
     gene            complement(29214..30398)
                     /locus_tag="PK898_04695"
     CDS             complement(29214..30398)
                     /locus_tag="PK898_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU19366.1"
                     /translation="MGNLGSIVDTFYLLSWREIVMIIVGLVLIGLAIFKEYEPTLLLP
                     IGFGCVLANLGMSAYQTFTVTEVVNGQATEVVRSGFLKVLYDAGITTELFPLLIFVGV
                     GAMIDFRPLLAMPKMVILGAAGQFGIYGTLLLASFLAKSFPGLHWGLAEAASIGTIGA
                     IDGPTAIYVTSKIAPNLLGPIAVAAYSYMSLIPIIQPPIMKALSSKEDRKIRMEYAPR
                     EVSDRSIVVFPMLVTIIISIISPAAAPLIAALMLGNLLKESGVVDRLDKAAQNEIINV
                     STLFLGLAVGGTMIGSQFLTVGVLFILVLGLLAFVLDTVFGMLFGQFMKVITGRKINP
                     LIGACGISAFPMAGRIAAKIALEEDPDNFILMHAMGANTAGQLGSVIAGGVLLALVQG
                     FL"
     gene            complement(30411..30818)
                     /locus_tag="PK898_04700"
     CDS             complement(30411..30818)
                     /locus_tag="PK898_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU19367.1"
                     /translation="MKLTVKVEDKTFDVEIADPNANPMTVKVDGTEFKVYGDSAAKPA
                     PVAHAAKPAAAPAPKPAAAGSDTVKAPIPGVIQEVLVKEGQTVKKGDVLFVLEAMKMK
                     NNILANRDGTVATINVHKGDSVTHGQVLLSFAD"
     gene            complement(30877..31224)
                     /locus_tag="PK898_04705"
     CDS             complement(30877..31224)
                     /locus_tag="PK898_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19368.1"
                     /translation="MEVEIVETTEKLIDNSHVMGQAGQITLIGILILFISLLILWGVM
                     EIIVRVIKDPEKAEVIEEVEAEIITPVVEMVDENVLKMQAAAAAVASIQNYDLKMQVA
                     AAAAGYVAAKKES"
     gene            complement(31252..32787)
                     /locus_tag="PK898_04710"
     CDS             complement(31252..32787)
                     /locus_tag="PK898_04710"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOU19369.1"
                     /translation="MSDSEYEELRRRRQQILDGGGKERVAKHHAQGKMTARERIETLL
                     DPDSFLELEPYTLSRFDTKNKYYGDGVITGFGTIDNRTVYLYAQDFTVNGGTMSEIQS
                     RKICHLMDMAKENKAPVIHLIDSGGARIQEGIVSLAGYAGIFMRTTGLSGVVPQISLI
                     LGPCAGGAAYSPATTDFIIMTRKKSFMFLTGPDVVESVTGEQIDSETLGGSEVHLAES
                     GLAHLAADSDREALEYCRRLLAYLPDRWHGISADLPADDPIDRMDPFLNSIVPADPAE
                     PYSMHQIVETITDRDSFFELQAEFAPNCIIGFARFNGTAVGIVAQEPSIMAGVMDINS
                     SDKISRFVRTCDCYDIPIVTLVDCPGFLPGVYQEHHGVIRHGAKVLYAYAESTVPKVT
                     VVTRKAYGGAYIVLGSKMMGSDVSFAWPQAEIAVMGPEGAAKILYRKQIEAAQDPDAE
                     YRRLVEEYRKKYLNPFVPAETGFIDDVIEPQETRKKIIKALSGIKRKKIVPEERKHGN
                     MPV"
BASE COUNT         8579 a         8614 c         7420 g         8251 t
ORIGIN      
        1 attcgctttc ccaaaaattg gattcttccg agcaaactcg tacaaaattg gtttcttatt
       61 tgactcacga actgggacgc ccgttgggcg gactttcatc agccgtggat gcacttcaga
      121 tgggtgcggc acaggatgaa aaactggttc aggatttgac cgtcgggatg aaaaacgaaa
      181 ttaaacgttt ggaattgcta gtgggtgata tcgcgctttt gcgggaaaaa acagatccca
      241 tcaacctata tgtcatgaag ccggtcccgt tgtccgcttg gttgaacgaa gtctacagct
      301 attgggccga atatgcgcgg gataaagata ttaaactggt cgatgagatc gatcagaatc
      361 tgccgacgat caaaatggat gaaaaccgca tgtatcaagc cgtcgggaat ttattgagta
      421 atgccattaa atattcaccc aatgggaaga ccgtcacctt tcgggcgttt cagaccgatg
      481 acgagattca aattcaagtg accgatcaag gctgcggcat cgatcgagaa gatcttccgc
      541 atatttttga atccttctat cgaggttcgg agaataaacg catcattcag ggaatggggc
      601 tgggtttgac tatctctcag gacgtcgtcc aagctcatcg agggacgatt actgtcgaaa
      661 gcgagaaagg cgtcggcagc actttcacca ttcaccttcc gctcaagaac atttcacccg
      721 aaaaatagta tgaaccggac aggtctttca atccgacaac aactcgtttc catggctgac
      781 gatcattata aaatcttcag ttgcaagctt atcccgaata ttccgccgga acgaatttta
      841 ggagttcgtc tccccgattt acgaaagctt gcgcagcgga ttgccggcga agatcccgaa
      901 agtttttttg ccgatgccgg cgatgaaact tttgaagaaa aaatgctgca gggaatggtg
      961 atatgctatt tcgacggtcc tttcaacgag aaattggctt tggttgagaa atttatctat
     1021 aaaatagaca attggtctat ttgcgacagt ttttgtgccg gcttgaaact tccgttgaaa
     1081 tatcccggag aaatgtggga ttttattact ccttatttac aggatcaaag agaatatttc
     1141 atccgtttcg gatcggtcat gctgctttcc tattatgtcg atgagactca cttgcacgct
     1201 gctttatctc tgcttgatca agtcagaagc cgagactact acgcgcgaac gggagtagcc
     1261 tgggcgattt ccgaatattt ttcgcatttt cccgaagaga ccatgcgcta ccttcatgag
     1321 agctccttgg atgaccagac ctttaataag gtccttcaga aaatcatcga atctcgcttt
     1381 acggatgcca aaatgcgtga ggaaattcat gctctgcaac gataatacag ctttaataag
     1441 ccaaatttct gcttaactcc caccttaaga taaaaataat gttcttcttc aataaaaatt
     1501 aatgttctat taatattttc gtagtaggct taataatgat caaattagag gaaaatgcct
     1561 tatttaacaa aaaagaaata agaaagggta aaaaaaatat gaaaagcaca actagaaaca
     1621 ctattttgtc gattcttctt acattgctga tagttttcag tgctttttct ccggttcttg
     1681 ctcaaagtca aaccgctgag caaacgatca gcgaaactag cacttcgttc agcaaattgt
     1741 atgaagaagt caatccctcc gttgtctata tttccgtagc cattcaggga gggtcttcca
     1801 acacccaaca attccaagga ttgccggacg atctggaaga attgcttccg tttctgaagc
     1861 aattctatgg tccgcaggga caaaacaatc aggatcagga atcggaaccg aatcagaatc
     1921 agaatcagaa ccagaatcaa aatcagaatc agaatattac ttatggatca ggaaccggtt
     1981 tcgtctggga tacggaagga cacgttgtca cgaataatca tgtcatcgaa gacgcaactg
     2041 atatcaccgt cacttattat gacggaatcg aacggaaagc gacggtaatc ggcgccgatc
     2101 cggacagtga tttagcggtt ctgtcggtgg aagattacag aaaagatatc gctcccgtga
     2161 aaatgggtga ttcagatacc gtaaagacgg gcgaatatgt tgcagcaatc ggcaatccgt
     2221 tcggaaatgt cggaaccatc accatgggaa ttgtatctgc tctcggccgt aatttcccaa
     2281 ttgacgatgg gtcaacgggc agtggcggac attataccat tccggacatg attcaaacgg
     2341 atgccgcaat caacccgggg aactcaggcg gcgttctgat taacctaaac ggcgaagtca
     2401 tcggagtggt taactcattt gtttcagcca cctattcatc agcgggaatc ggatatgcca
     2461 ttccatcgaa cttggcggtg cgtgttattc caaaactgat cagcgacggg aaatacgaac
     2521 atgcatggat cggcatcagt ggtatcgcgt tgaatcctga tatcaatgcg aaactggaat
     2581 tggatcgcga tcagcggggc gctttagttg aaaccgttca atccggcagc ccagccgata
     2641 aagccggaat taaagctggc gacgaaacaa caactatcaa cggcgctgaa attacaatcg
     2701 gcggagatat cattaccaag atcaacgacc gagaggtaaa aagcatggat gatattannn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga gatatcatta ccaagatcaa
     2881 cgaccgagag gtaaaaagca tggatgatat tatttcatac ctggctttcc atactgaggt
     2941 cggagataaa gtcacactgc atattctgcg agatggaaaa gaacaggata tcgaattgac
     3001 attagctgcc cgtccgtctg tcgaagaacg caccggcgtg aaagcaaatg agaatactat
     3061 tagagagcag gtcggtgaag cttggatcgg aacttatgtc aaagatatat ctgccaacga
     3121 tatcaaaaaa ctggagctgc cggaaggaac caccggtgct ttaatcactc aggttaccaa
     3181 agacagcccg gcggatgatg cgaacttgca ggaaaacgat atcatccaga aaatcgatga
     3241 taccgaaatt aaatcggtgg atgatctgaa agccgaactt tcgaaatata tgcccggcga
     3301 acgagtgaca ttgacgattc tgcgcgacgg taaaaccaaa gaagtgcgct tgacgctcgg
     3361 tacaaccaag aataaataat cgcaaagaga ttcagaaaga tccgctttct gaatcaaacg
     3421 atcaaaaaca agactgacga aatctgtcag tcttgttttt ttaacatcga atcgcagttc
     3481 aaaagggacg gttcaaaaaa ggattaatgg aacctgcatt tcatcttccg tcaagccggc
     3541 atgatgccct ttaaattcct tcggaggctt tgccccgggc ggagcataca gcagatcact
     3601 gtgaccgacg cccaaagcga tgaaacttcc caaaaaatct ttaactttcg ggtgaagcgg
     3661 accttcgccc aaaaagcccc gacgaagaaa ttcatccgcg ctgagcaaaa tgaattcatc
     3721 accgagttcc cgttcaaatc gggaggcaaa cagatcttcc ttccccggta taaccgatat
     3781 cgcttttgcc cgtggatccc cggacggata atggttcatc agctcggata acggagatat
     3841 ttgatccaga tgaaaatgat tgaaaatatc ctgcatcccg tgatcggcgg aaatgattcc
     3901 cacggcatcc gaaaatctcg gagcatattg ttccaacaac gaatctgttt tgcttaaaaa
     3961 ttccgttacc gattcatgcg aaagaccgtt attatgcaac agatcatccg gcgatttcca
     4021 ataaccgatg atgaagtgat ccccttcttc ggcacatttc aactcgatat tttgaaaaag
     4081 ctcttccaac gtatgggctt tggtcagacg atcccccgca atttgaacaa gtcgctgccg
     4141 ggcaacatcc gtaaaaatac aatcgatctt tattttccga tcggcattgt tttcaatctg
     4201 ctcaaaaata gtccgaaaac ccataaaatc caaaatattc ttttcgtttg gatcaatcgg
     4261 ctcgccggta aaggcgtccc gataggggaa aacctcgatg cattcatccc attctttaaa
     4321 atataaagac caagccaacc aggcatgtga aacaggctga agtcccgaaa aaaaagaaga
     4381 ggtcacacaa accgtcgtgc aagggtaaac agccgtcaaa tcccgccgcc ggtggctgcg
     4441 taaaaaactg tcttccggca aatgacggtt aagaatgccg gtccccatgg catcaatcaa
     4501 aagaaaaata acattgcgcg ggcgcttttg agcgagcaaa gaatccacat ccgtggaaga
     4561 ttttgtggaa gaaggtacag catagtaatt taaaaccgat gaaataatcc ctaaattgga
     4621 atggttataa tccgggcaaa caatattttg ttgaaaattc atttcctttc cttaaagtaa
     4681 ataatcgata cattgtttca gcaacaatcc gctgaccacc acgataaata aaatgcgaat
     4741 aaacttgctt cccttaagaa ccgcaagccg cactccgaca taacttcccg ccatattgaa
     4801 tacagccata atcaaaccga ggtaagcaaa aatcgctccc tgtgacccga aaaggatcaa
     4861 tgccccgaag ttggtagcca gattgataat tttacaggat gccgaagccc gaagaaaatc
     4921 catcccgaaa attccgatca acgcaaggat caggaaattc cccgtaccgg gaccgaaaaa
     4981 tccgtcataa aagccgataa caagacccgc cagcaggctc aatccgagtt gttgcctgaa
     5041 agaaaaagaa acttcgtgca ccctcacccc gaggttctta ttcaatatcg tataaatcag
     5101 gacaaccgtc aacaaaacca agaccaacgg acgcatccat tgattgggga ccgaaaccgc
     5161 cgtcatggca ccgaagatcg atgaacaaaa tgcgacgccg gcaatgggaa gcactttctt
     5221 ccactcgata ggaacttttc tcgaatattg aaaaacagcc attccggtcc cgcagctgga
     5281 tgaaagttta ttggtaccga gcagagaagc gaccggtgca taagggagta tggccatgat
     5341 tgccgggagt tgaattagtc ctcctccgcc cgcaaccgaa tcgatcaaac cggcaagaaa
     5401 agcaaatccc gaaaggatta atatttgaag aattgtaata tccggcatgg aattgattcc
     5461 aatctcctga gagcgattta accttttcga ttataccctc agtcccgtcg ttttcccgtc
     5521 gccactcttt tttttcattc atttcggcgc aaaattcaaa aaatggaaaa atcaccgcga
     5581 aaaaacacga acccttttcc caatccgatt gaagtagatt agaattacac gcataaccat
     5641 ttttgcgtcg gagaaacaga catgagctat cagggcatca ttcatcgcta tcacgaattc
     5701 atggatcttc cgcctgcggt gcaaccaatc actttatacg aagggaatac ccctttgatt
     5761 cccgtgccgg cgatcgcaga caatttaggc ggcggtttcg agctgtatgt caaatatgaa
     5821 ggcctgaatc cgacgggttc gttcaaagat cgcggaatga caaccgccat gacgatggcg
     5881 aagcataatg gtaagaaaac ggttatctgc gcttccaccg gaaacacagc cgcctcggca
     5941 gccgcttatg cgagccgagc cggtcttcgt gccattgtca tgatcccgga aggaaaagtc
     6001 gccgccggaa aactggccgg tgcggttgct tatggagcgg agattatcca gattcccggc
     6061 tcattcgatg acgcgctgtc ttttgtcgta gaaatcagtc aaaaaagtga aatcgaactg
     6121 gtcaacagtc ttaaccctta tcgcatggaa ggacaaaaga ccgcttcttt tgaaatttgt
     6181 gacgagctgg gaagcgcccc ggactggtta tgtctgccgg tgggcaacgc tggaaatatc
     6241 acggcatatt ggatgggatt taaacagtat aaccggataa aaaataccgg cctgccgcat
     6301 attctgggtg tccaagccga aggtgcggcg ccgcttgtga tcggacatac agtggaacat
     6361 ccggaaacgg tggcgacggc gatccgaatc ggcaatcccg cccgcggaga gcaagcttta
     6421 atggccgcgg aagaatcaaa gggacggatt atcgccgttt ccgatgaaaa gattttggcg
     6481 gctcaaaaat tgttggcttc atccggtatc tgggtggaac cggcttcggc tgccggtctg
     6541 gccggattaa ccgaccgaat taaaaacaat tccttcgatc ccaaaggcaa acgaattgtg
     6601 cttgtctgta ccggacacgg tatgaaagat cccgatatca ttatttcccg tgggattaaa
     6661 cccgttcgtc tgccggttga tttcgataaa gtggcggaat acatcctcgg aggagggaac
     6721 cgatgagatc tttttccgtt tatgttccgg cttccaccag caatatcgga cccggattcg
     6781 attgtgtcgg attggcggtt aacctgtgga acgaagcacg gttttatttt gattcctcgc
     6841 tgtcatcgat cgaaattaca gcggaaggct accaaagcaa agtccaaaaa caaccgagtg
     6901 aaaatctgat ctttcgttcg tttcttcatt ttttcgaatt aaaaggaatt aaggttccgg
     6961 aaggactcag aattcattgt gtgaaccgtg tgccgatcgg gtcggggttg ggctccagtt
     7021 caaccgcgat tctgataggg ttgctcgctg ccgatgagct gctggggaca aagacttcac
     7081 aacagggaat tcttgaaatc gccgcaagcc tcgaaggaca tcccgataat gttacgccgg
     7141 ctattttcgg cggtctgacc gctgcttcga tggacgaaac gggtgttcgg acagaacgct
     7201 tcccgatcgc cgattggaat atggcggttg tggttcccca tttcaacctg tcgacaaaag
     7261 cggcccggaa agctttaccg gatacggtcc cgttccggga tgcggttttt aatctgaacc
     7321 atgcgatctt tttaatgcgg gcacttcagg atggggatga agaagaattg cgatttgcca
     7381 tgcaagatcg attgcatcag cagtatcgaa tgcgattaat cccgggatcc gaagcaattt
     7441 tacaagccgg agtcgacgcc ggtgcagcag ccacggggtt atcgggagcc gggccgggcg
     7501 tcattgcctt cagcatggga aaaacagatc aaatcctggc ggcaatgctt caggcggcac
     7561 accttgccgg ctatgaagcg gacggtttcg aattaaagac ctcatccaac ggagcatgga
     7621 taatgcctct ctaaaagaag gaggagacat gaaaaagaaa ttaattatta ttgcgattgt
     7681 gctgatttgt gtaaccggtt gcgggaagaa aaaaattgcc gatccgattc aacctttgac
     7741 gaaagtaacc atcagtcccg aaagagaagc cctttacgaa gcgacaaaag aagcttggct
     7801 gacggtaaat cccgttgaaa taaagagcac ccaatccgac catgccacac cggttccgca
     7861 agaccaagca ccgaaagccg ttgagtcaga gtcgatcgtt ctggcaaata ccaaaccggc
     7921 tgcggtaacg gaagcgccgc agggatttta tcaattgcag gccggcgaaa caccgaaatg
     7981 tatcgcccgc cgctttaatg tcgattggct gaagctgtac tcgatgaata acatcagttt
     8041 tgaaaatgaa aacgatattc aaccgggaac cgttttagtc attccgcaaa attccgtttg
     8101 gaatgaaaaa cacggtcctc gggcttcgtt gcctcatccc accgactaca atgtccaagc
     8161 cggcgacaca ctgaacagta ttgcctgcca attcggagat gtgacccccg aacaaatagc
     8221 gcttgaaaac ggcattactt cacaagaaca attaattccg ggattgagcc tgcatattcc
     8281 ctgatgcccg atcgaaatga ctgaaccgga aaccgaaaaa tcctggcttg aaactcccca
     8341 tgggcggttg gattatcctg tctatctgcc ggatgcaacc tacggaatgg tgcggggagt
     8401 cgatgcggta gatctggagc aagccggtgt acaggggctc gtgatgaata catttcatct
     8461 gatgcagaag cccggcagtc tgaccgttca gtctcttggc ggcattcaca caatgagcgg
     8521 ttggtccaag ccgatcatca ccgactccgg cggttttcag gcatattcac tcattcgcga
     8581 aaatgccaag ttcgggaaaa taaacgaaaa cggaatcacg atcacaccgg aagggtcgaa
     8641 acggaaactc ctgctcagcc cggaaaaaac tgttcaattg cagatgtcat acggatcgga
     8701 cgtggtaatc tgcctggatc aatgtaccca tcccgatgat tcggaccaag tgcagcggga
     8761 atccgtccgc cgcacgatcg aatgggcaaa acgctgtaaa catgaattta ccaaacgctt
     8821 aatacacacc gaaaaagaca caaaaccgcc gaaaatcttt ggggtggttc agggtgggaa
     8881 ctctttcgaa ttgcggaaaa cctgtgccga cgcattgctt gaaattggct ttgacggctt
     8941 cggattcggc ggttggccgc tggacggaga aggaaatctg gttgaggata ttttgcaata
     9001 cacgcgggaa ttaattcccg ctgaatatcc gctccatgct ttgggaatcg gacatccgga
     9061 aaacgtcgtc cggacttttc gattgggata tgatatcttc gattgtgcga tgcccacacg
     9121 ggacgcacgc catggccgaa tttatgcttt tcgcgaggat ccgccgcaat tggaaggaaa
     9181 atggctgcgc tatctttata tcgatgacga taaatatatc cgctcatccc agcccctttc
     9241 tccggggtgc gactgcctga cttgtacacg ctactcgacc ggatatttgc atcatttgtt
     9301 caagatgaat gacagcctct atcccagatt ggccactatt cataatattc gaatccttac
     9361 ccgattgaca gagctgctgc gtcgtgagaa atatggaaaa tgattcatcc gtcctccaaa
     9421 tcgccgccga gatatccgcc ggaaagaact tcagggagat cgatcccggt ttgatccttc
     9481 atttggtaaa gacggaaatg caacccaatg aatccgaaaa ggaaaccgtc aaacgggtaa
     9541 gatcgaaact tcatcaaatt tccaacgctt atcgggaagg ggtaagcaac tatcaagacc
     9601 ttattacggc gatctggcaa tccgataatt ctttgacttc tttagaagct gccaagcctt
     9661 tggcaatcga actgatgaaa tcgcatgcct cgacccgtga gagaatcgca atattagatg
     9721 tcttttactc ccaaatattc gctgcgctgc cgcccatcca atccgttttg gatctggctt
     9781 gcggattgaa tccgttagcc atcccttgga tgaaattagc cgatcatttt acctatgaag
     9841 cttatgatat ctatcgggac atagcgctgt tattgaatgt tttttttgaa aagctcaata
     9901 ttcggggaaa agccgattcg atgaatctgc tggaagaatt tcctcaaagc gaatgtgatc
     9961 tgacgtttct attgaaaacg atcccctgtc tggagcaagt cgataagaat gccggaaccc
    10021 atatcctgaa tcaaatccgt tctccctatg cggtcgtcag ttttcccatt ttcagcctga
    10081 gcggcagaca aaaaggaatg aaagttaatt acgaaaagca ttttttggaa atatacgacc
    10141 cgcaacgttg ggaactcgaa agaatgctgt tcccaaatga gttggttttc ctgatgagaa
    10201 gaaaatgaac aatcgcaaag aaacagacga tacggtcgat caaattgtcc gcaagattct
    10261 ctcatcacca aagtatcggg aattgggaat tccgaaagaa agtgtcgaag atattgtcaa
    10321 acgggaagtt tcccgacaaa ccgacatcaa agttgccgag aaaaaagcgc gggagaagct
    10381 gcaccaagtg gtcgcccctt atttgggcga tccggactat cgctcagccg gtaccgcatt
    10441 gacgcaaatt catcgtgatg atcgggaagg gctcaaagcc tggtgcctgg aaatgctccg
    10501 atcccattca tccacccgcg agcggctgcc gaatttggag ctgttctatc agactgtctt
    10561 tgaattcacc ggaaaaccga atactgttct tgatttggct tgcgggatga atccttttgc
    10621 tttcccctgg atgggattgg aaccgtccgt ccgctattat gcctttgact tgcacactcc
    10681 gcgagtaaat ctgatcaatc gcttcttcga cgcatgggga ttacaaccgc tggcaaccgt
    10741 gaccgatgtc ttggttcagg ttccgcaaac gccggcggat gtcgctttct tttttaagga
    10801 agcgcatcgc ttcgaaagcc gccggaaagg atccaatcgg gctttttttc aacaactttc
    10861 ggtaaaatgg attgtggtca ctctgccggc agaaaacctg accggccggc atcaaatgcg
    10921 tgatcgcgaa cgtatgctga tagagaaaac aatcaccggt ttggattggg aaatcagcga
    10981 aacggaagtg gggacggaaa tgattttctt catcaggaaa tatgagtaaa aaaaagaatt
    11041 cggaaagaag ccgaaacatc tcccctcgaa aagaggcttt tgacaccgcc tgggaaaaat
    11101 tcgaaccgct gattcccgaa gaggaattgc cggcattaaa ggcatgtatt caatttacgc
    11161 cggacccggc gattcgatta aacccgcaaa aagcaagcat atcggatatc ggaaaattac
    11221 gggagcgata tcattgggac accgaaccgg tcgctttttg tcccgacggt tataaattaa
    11281 aagataccca cggaatcgca cccggccaaa caaatgaaca tcgctgcgga gcattctata
    11341 ttcaggacag cgcctccatg ttgccggtta cactttttga tcggcaaaag attctgaaca
    11401 agccattgat tttggacatg gcagcttcgc ccggcggcaa aacaacccat ctttccgcgg
    11461 caagccggga ttcgggactg attcttgcca acgattccag cgcctcccgc atcacgatat
    11521 tgaaaaaagt gatgaaaaat tggggcagta ttcatcatct gatcaccgtt tttcccggtg
    11581 aatttttcgg acaatggtat ccgaatacat ttgatctggt gttattggat gcgccctgca
    11641 gcatgcagag tttggtttcc atcgcttctc atccgatgcg cccgatcagc gaacgggaag
    11701 aaaacgcact ggcgcaacgc cagctgtcgc tgctcgacag cgccttgaaa gccgtcaaac
    11761 ccggcggtca ggtcgtttac tcaacctgca ccctgtcacc gacagaagac gaatcggtaa
    11821 tcgatgggat tctgaaaaaa tacggtgcgc aaatcaccct gcttgacgct caacagaagc
    11881 tcccatatcc ggcgccgggt ctggtatcgg ttgaaggatc agagccgaat ccgtctttgg
    11941 aaagaacaat ccggttgtgg ccgcatcgtt atcagaccac cggatttttt gccgcattga
    12001 ttcaaaagaa tgatactttc ggattttcgg aacaaataga aaaacccttc cgtcgatcac
    12061 gcgaagaact tcgattcatt gaaatcgatc ccagacaaga tcatcatccg atcgagcgca
    12121 tgaacgagat tttcggattc gatcttaccg agttcgcgga acaaagagaa tgttgttttc
    12181 ggatgcgcgg caatgaactt tgggctgtcc caacggtatt ttttgacgaa tccgtcgatt
    12241 ggccgccttg caaatcctgc ggaatgcgag tcgccgccca gacggcctcc ggatggctcc
    12301 cggactttga ttgggtcagt ttggtttttg atcgtatttc ccaaaaccgc tatccgttgg
    12361 acgaaacatt ggcggaggca tgggttcggg gggaagattt gcccatatct ctgccggaac
    12421 actccaaggg gacgatcctg tttatgacgg attcctggaa cgtattcatc ggttgcggat
    12481 atgtcagcgg aaatcggatc cgcaacctga caaaataaaa agacagagga ttttttctct
    12541 gtcttttttc ccgaatctca tcagaaacgg tctcttttat aacacattcc acggattcac
    12601 gtatccgccg ccgtatcgca cttcaaaatg aaggtgagga ccggtggaat ttccggtact
    12661 gccgcccaag ccgatcacct gaccttggga tacggcctgc ccgcaactga cgttgaccgc
    12721 ggacaagtgg ccataaaggg attgataacc gtttccgtga tcaatcataa ccataatacc
    12781 gtacccgcct ccgatcggtc cggcgtatac gaccgtcccg ctgtccgtgg cgtaaattgg
    12841 accaccctca accgaagcga tatccaaacc catgtgcccc gaccagaaat cattgcccga
    12901 taccgagtgg ctggccgaag gccagacaaa agtgccggtt ccgtaagcga cataatccca
    12961 ggcacatccg ccgggtccgg aaatctgctt cttgacgccg gaattcttgg agtactcaaa
    13021 cacaatcggg ctgataaccg ccgtctcgcg gtagccgccc ggcaacagga taaaatctcc
    13081 ggcttttatt tccggatttg tcatgtccaa atggttggct ggataggata atatatcttc
    13141 tacctgaacc cgatatttgt ccgcgatctt ttgcaggtca tcattttctt tccattgata
    13201 atatatcccg tcaccgggcg gaataatcag ctcatccccc acggatatat tatgggcatc
    13261 gtcatgcaaa acattataat tggaccaaag aatggtttcc ggcttcaaac cgaatttttt
    13321 ggcgatcccg taaatcgagt caccggcttg tatggtatat ttctgcggtt ccaaattccc
    13381 gcgatcgggc ttatccgtcg tcagtttggc tttgcgttcc acggaatccg atgattgaac
    13441 ggtaattgga ttgagctgat ccggcaaatc cgtattattt tcgacttcat tcacaagctg
    13501 agcgccgaca ttttcggaga cattattgtc atccgccatc ccccggatat agggagtcct
    13561 ttgctccata acgactccca tgaaaaaaac gcaaacaaga gcaaccgccc aaagggcata
    13621 agggagccag gaaggagttt gttttttccg ctccggcatc cctgattgaa tcctcacata
    13681 tcctccgtca aattctccag gaattcgtta ttcgtcttcg tcttcatcat tcgttgaata
    13741 atggcttccg tcgccacagc cgaatccatt cccgcgcctt gcggcggttg accgatcatc
    13801 tgcatataca tccggcgcat cagccagact ctcggcagca gatcgggacc cagcaaaagt
    13861 tcctcccgcc gtgtccccga tttttcgata tcgatggcgg ggaaaattcg ccgttcctgc
    13921 aattttcggg aaagatgaag ctccatgttg ccggtgcctt tgaattcctc ataaaccaca
    13981 tcatccaacc gcgatccggt atcaaccagc gcggtggcta aaatcgtcaa cgatccgcct
    14041 tcttccaaat ttcgtgcggc gccgaaaaag cgtttcggcg ggtacatggc ggacggatcc
    14101 attccgccgg aaagcgtccg gccggatgga ttcactacca tattataagc tcttgcgaga
    14161 cgggtcaagc tgtccaacag gatcacaaca tccttgccga tctcaaccag tcgtttcgcg
    14221 cgttccaagg taatttccga agttcgcaca tgttgtgtga ccggttcatc gaaagtggag
    14281 gcaaccactt ccgcatccac cgaacgatcc atatccgtca cttcctcggg acgttcaccg
    14341 atcaaggcaa taatcagatg cacatccgga taactggaag aaatcgcatt cgcaatctgc
    14401 ttcaaaatcg tcgttttacc ggccttcggc ggagaaacaa tcatcccccg ttgcccgcgg
    14461 ccgatcggag cgatcaaatc gatcagacgg gtggcataac tggcgggatc atattcgata
    14521 ttaaaccgtt cattgggaaa aatcggagtc atggactcaa acgtttttcg gttcttcgct
    14581 tcttcaggag actgtccgtt aatggattcc acccgtaaaa gtccgtaatg ccgttcggat
    14641 tcccgcggcg gtcgaacaaa accgattact tgatcgccgc tgcgtaaatt ataccggcga
    14701 agctgcgcct gagaaacata tacgtcttcc ggtccgatcg cataatccgc acgcaaaaaa
    14761 ccgatccctt cgtttaatat ttcaaggatc cctccccgaa cttcaacgcc ttcctgttcg
    14821 ccctggactt gtcgaatctt cacgaccaga ctgtccttct tcaatctctg ggcgttcgga
    14881 atattcatgt cctgagcgat tttacgaagc tcggcaagtg tcatattagt aagtgattca
    14941 tttttcatag ataatttaat cctggatata taaagtattt agaatttggt cggaaattcg
    15001 gaggtcaaaa agccgttgca ggttcaacgc atgcaaatcg ggaaaaactc agaatttcag
    15061 ggatttgttt tttttgcaaa tagtatgttt ctcggggtcg cacccaggtc tactcacaat
    15121 cgtgaatatc ggttaatcaa tttataaaac agcaaaattt tcaagaaata tcatttattg
    15181 aggcaaatcc attatagcac tgaaacaaat tgaatgccaa attatattaa taattatacg
    15241 ttaaatatta tccggaagtt tcaatccggg aattttgctc cattcttccg ttaattcccg
    15301 aatccgtgga ttatctttat aatacccgcg atattgttcc ggcaacatag ccgcgatgcg
    15361 acacattaat tcggtggttt cttcttccat ggattcatcg cgtcgttccg gatcgatctt
    15421 tttcatggta taaggcgggc tgaaacgcat ggtgattttc ggctttcgaa aatggcgaaa
    15481 atcgtccata aaatgttcgg tgccggtaat actgcccgta caaactggta ctcctgattt
    15541 catggcaagc agaacaattc ccggtttccc tttgatcagc tgtcctgtcc ggctgcgggt
    15601 cccttccgga gccaatacca gcgattttcc cgctttcagc cagtcgaagg cttttttgaa
    15661 agcagtaaag tccgctttgc tgcgatcgat ccagatcata tcgatcgcat cacaaatctg
    15721 tttaaaaacc ggccatgatt gatatgaatc ggcgatcaat gcgctccaat cgtcacgaaa
    15781 tccttttgtg gcaaggaacg ggaaatccaa ccggctcata tgattaatcg caacaataat
    15841 cgggccctct ttaggaacaa attccagatt gacataagac ggatgggtca gcaacttata
    15901 agcatgatcc agaaaattga aaacggattc tctttccaac gacacctcac atccggacgg
    15961 attccaaaaa gtccggtttc aaccgagaac tttttggaat ccttttccgg aataattatt
    16021 ttgctttttc ttcgattgtc accgaaagaa ttgaatcgga agtggtcaaa gtttgcgcca
    16081 catcataccc ttccgaaact tttccgaaaa cggtataact atcggacatt ttattcagtt
    16141 gatcctgaac ttccttctcg atcatctcat cggtcagttt attttcggaa gtcagtttat
    16201 catttgcttc tttgatggac tgctgatagt aatcgtgcag attgaacgta atgaagaatt
    16261 ggcttccgtt tttatccttg cctgaattcg ccatacccac aacacccgga tcgccaaatt
    16321 tcagatcatg tttttcgttt acgaattgat atcccggatt gcccattccc gaaccgctgg
    16381 ggtcacccga ttgaacgata aatcccggga caactttgta aaacggaatt ccgtcaaacc
    16441 agccctgcgt tgccaaaaag ataaaattgt ttacagccag cggtgcttgc tcggtataaa
    16501 gatcaacggt gatcttgccc tttgtcgtat ccaacaccgc ccgatagtcc ttatttacgt
    16561 cgataagatt cggcggacat tcctgaaatg ctttgccttg caattcgaag aatttcaggt
    16621 agctggtcag cgcagcttca tcatacgaac cttgatagct gttgaaattc aagaaaatcg
    16681 tcggtgtacc ggagaccact cccgtcgcaa ccacttcgtc aaaagcctta tcgatcttcg
    16741 cccttaaatc cggatcggca aaatcggtca gccattgatc atagtttaat cccgaaatct
    16801 gagtcttgaa attatccttt aaccaggttt cgaaagcgtc caaagattcc agcccgctcc
    16861 aatcggattg agtggaataa agcagatgtt ccgcttcgaa gaaaaatccc tgcgcaccgg
    16921 cggcatccgc cgcgtaggct gccatcggcg ctttttcatg aaaactcaat gggaagtgac
    16981 ggtaaacata gcgaacttca tccggatgat tcttttggaa ttccaacaga gccagcccgg
    17041 caccggagca gtaagggcat tggaaatccg catattccaa aatcgtcagc ggagcgtctt
    17101 tcggtccgat gctccagtcc tcatccgtta tcggaggtaa cacagccgta attttttcat
    17161 tcggaccgac cggtttaaca atggtacatt ccatcggttg aacgttgtcc tgaccggctt
    17221 caaagggaac ccactcatct tcggcaaaaa caacggtgcc gaagatcaaa ctaattaaca
    17281 ccgcaagaat aacaatacct aaattctttt taagcataga tcattttcct ttcagaatag
    17341 gccttacaca agatgatttt ataccaaatc ttttcgggat ggtatcttta acaaagtttt
    17401 aagcaaataa ttacggctac agacccgttt taattacttt ccgaatattt ttcttcaaaa
    17461 atgaaggcga cgggcaagcc gttgcataat ttcattttcg acagcttctt cggattgatc
    17521 ggcatcgatt atctcccacc gttgcggatc ggcagcagcc aattgatgat agccattgcg
    17581 aacccgctca tggaacgcaa gctgataatc atccatccga ttccattctt cctgattgtt
    17641 tattctgcgt ctcaatcctt tttcggcggg tatatccagc agaaacgtca aatccggcca
    17701 aaggttgccg gtggcaaaat tgagaagttg ttttaatgtt tcctgttcaa atccgtgacc
    17761 gtatccctga taagccaagg ttgaatccgc atagcggtca ctgatcacaa tctttccgct
    17821 ttgaagggcg ggacgaatca cctcttccac cagttgagcg cgagccgcac aaaaaagcag
    17881 gatctccgtc cgcgggcaca tctgtttgtt tttcatattc atcagaattt cccgcacctg
    17941 atcactgatt tcggtccccc ccggttcgcg ggtgtagacg atatcaaatc ctttttcgat
    18001 aagcttccgc ccaattcgtt tcacttgcgt ggtttttccg gatccgtccg gtccttcaaa
    18061 ggtgataaac atgttttact ccggattctg aaaaatgcgg attctgcgtt tgggctcttt
    18121 cccgtaagac tgggataaca acccgtactg ttcgaccaat tcgtgctgca tctgccgaat
    18181 catgcgattt gcgggcgcca gatcaaccca gaccgcttcc ccgtttttca cggccataat
    18241 ggcttccgta gtttcttttt caacgttttc ccaattaccg gacggctgtt gaacggtcac
    18301 attaaacaag ttcgccaacg tgtattcgat ctgattgatc gtgtttgacc gcagaacata
    18361 gatcggcaca ttccgttctt ccgcgtccac aatcattttc tgccggctgc ggtaataggg
    18421 acgtaaagtc attaagacat ccgcttcctc gacgttattc acaatggaaa tcaagactcc
    18481 cagccttgcg gaagcctgtt ccaaacggtt acgggctacg ccatggggat aaatccggat
    18541 gggtttcatg cccggatcat tttgagaatc cgaatccaga tcccgttcac cggtcaaaac
    18601 cggtgatgtc gattgggtca tcccatagga ttccccggat acgccccgcc gtgaactccg
    18661 acgattgccg ctttccgttt tactgcgacc ggaaatctga agaatattcc gcttttggcg
    18721 gatttgcaga tcggactcgg ctgcgggtgt aaacccgttc ccttccaata cgggagccgg
    18781 cgccttttcg cgtattattt ttccctgatc cgtctttatc cgcaactccg gtgccagttc
    18841 gatcccgcgg accaacgcat ccaccgccgc cgccacatcg ggataaacaa ccaccttatt
    18901 tcgttcgaca atttccacca gcacatcgaa agtcggcgga gaacgccgtt ccaaaaccgt
    18961 tttctgcgtt ccgcggcgtc gcgcctcttc atccgacaac gtcaccgatt cgattccgcc
    19021 gaccaggtcg gagagggtcg ggttcatcag cagattgctc aaggttcggc cgtgagccgt
    19081 cccgatcaac tgaaccccgc gctccgcgat cgtacgcgca gcctgcgctt ccaattcacg
    19141 gccgatttca tcgatcacga tcacttccgg attatggttt tcgaccgctt cgatcatcac
    19201 ctcatgctgc aaagccggtt tcgccacttg gaggcggcgc gcctttccca ccgccggatg
    19261 aggcacatcg ccgtccccgc cgatttcatt ggaagtgtcg acaatgacga cccgtttcct
    19321 ttccgccaac actctggccg cttcccgtaa catggtcgtc ttcccgatcc cgggtttccc
    19381 caaaatcaga atacttttgc ccgaatccac aaaatcctga atgatttcga gcgtcccgaa
    19441 taccgcacgg cccactcggc aggtcaatcc gatgattttc cctttccggt tgcgaaccgc
    19501 cgaaatccga tgcagcgtcc gttccattcc cgcccggttg tccaaatcga aatcgccgat
    19561 ttgggaaata atcgtctcta aatcttcaaa ggcgacttcc ttttcggaaa gctgaatctc
    19621 atcatccgta tagcgagcca agggcggacg tcctacatca aaaaccactt ctaccaattc
    19681 agatagattt ccgatacttt cgatcttttc tccgatctcc tcggggaaaa tcgccactaa
    19741 cgcgtggata tcatctaaat tattttcgta ctgcttcatt tttccttttt attcatctga
    19801 taatttgccg gtccgaccgg ttgaagataa ccgttttcca attggatcgc cggtttttcc
    19861 tcagccactt tcgcggcaag attttttaca aaaactgtct ggcgacatac cagattaaaa
    19921 ttttcctcat taaacggatc gtttgcccaa gcctgaaaac ccgggatcat cacataaacc
    19981 tcctgatccg tcgtatggtt gatttgttgt gcgaggctta gcattaactt tgccggattc
    20041 tcgacccccg ggttgaaaat aggctccaaa tagacccgat tcccgttttg agcggtcgcc
    20101 tttgccgtcg cttggatcat accatctttt ttaagcgcat agatattttc ctgattccaa
    20161 ttcgaaccca actggcgaat cagcggcgta atctgcgatt cgtagaacag atcgtattca
    20221 tcaaaaggcg taacgcttac ggattgccaa ttttctccat ccgaatttaa ttctccttta
    20281 taacaccaaa ccgtctgttt ggaataaacg ctgaatccga tctgacggaa tgaaccggtt
    20341 acccattgcc cttcatccaa aaccaccgca caagactgca ttccggcttt ccccgccttg
    20401 gccaccagaa attccaacaa ctccaacagg ttcgagttat tcgaatgttt cccggcaatc
    20461 aaccacgaaa accaagccgt atttttcttc atatcgaaac tgatctgccc gccccaataa
    20521 taggtcagat aaggcccgcg cgaaacggcg atcgattcgc ttgaaagcaa attgagagaa
    20581 tccaaaaaac aggttgtcag cgaaaactgg ttattggtaa aacgggttct gtttgaaaga
    20641 caataatatt tgttctccgt ttcaaggaaa aacttcatat cataaagtcg aaatgttctt
    20701 gcctgagcca ctacaatccg aaaattcctc tcaatcccgc cgtttaagga ttccgaaaac
    20761 caccacaatc cctactccga tgagaatcaa aacaatgccg atgatcgcca acaacggcga
    20821 tttcttggtc tgctgttggg tcgccgcagc cgaattttca aatgcggctt gtttttgcgc
    20881 accgaaattg acctgagtcg tatcacccgc tttgatcgta atggggtaat tcatattggt
    20941 cgtcgggtta tactccttcg gcggagcgac gctgatgtta tagtcccctt ccggcacctc
    21001 cggaaaacac aacagatcca atccggtcgt caaaccggtg aaggactccg tccctcctcg
    21061 tttcgtaacg ctaacggctc cgccggcgat tccggtttcg gtctcctccg cggtggcgtt
    21121 cccgttctta tcttcgaaca gatagataca gagttgtgcc gatccgtcat aaggcgtcgg
    21181 cgtcggtaaa atttcagtcg gcgtcggcga aggacccgcc gtcggtgtgg gggtcaggac
    21241 tctgcccaaa aggatcttcg ttccggcaat cagcgtgcaa ttcacatcca aattattcag
    21301 cgagcgaatc gtttcgacat ccacacccat cttcagtgaa atactgagac aggtatccaa
    21361 gtcggacact gtataaaaaa tattgccgtc aaaatcgggc gtcggcgacg ccaaagcctg
    21421 ataggcaaaa accggctgtt cgaagatcaa caacaacccc aaaattccca acggaatgca
    21481 cagcatccaa cgaaacgaaa ataatatttt tcttattcta ttcacgattc aaattatatc
    21541 atttgacgct ttctttccat ttttgggcat tcagaatcat ggcgccggac gcccgccggt
    21601 tttccgggct gtccccaccc attaattgcg ccatttcgcg cagactttct tccggctgca
    21661 attgtttaac cagtgtacga gttctgccat cggaaagttg cttggaaaca tgaaaatgag
    21721 cgtcataata agccgcaagc tggggcagat gagtaatgca taatatctga tgattgcggg
    21781 aaagcagcca tagtttttcc ccgacaatcg aaccgacccg tccgccgatt ccttggtcga
    21841 tttcatcgaa aatcatcgac gggatcgaat ccgcttcggc caatgtgttt ttcaacgcca
    21901 acatcagtcg cgaagtctcc ccgccggatg cgatcttggc caacggtttc aatccttcgc
    21961 ccgggttcgg cgcaatcaga aattcaacct gatcaaagcc gttctcatca taagccaatg
    22021 atctgccgtg ccggtctcgg agtccgtttt cagccgccgt ttgcgtaaat cggatctcaa
    22081 actgtgccga ttccattcgc agctcatgca gctccttttc cacgctctcg ctcactttcc
    22141 gagaagcttc gtgccggcga tcggaaagct cctgcgccaa ttcggataac aggttgatca
    22201 ccgaagtttc ctgccggatc aaatcttccg tctgatccga aatattttca agcccggcca
    22261 gctgttcttc cgcccgcgcg gcaaattcca aaaccgcacg caggctgccg ccgtattttc
    22321 tttttaaagt gttcaacagc gccagccgtt cttcgatggc atccagccgg gcggggttaa
    22381 attccaatgt ttcttggtaa cgctgtaaag aggcaagcaa ttcgttcagc gcatcgttta
    22441 tcgcggcgac ttcttcaccg agcggcgcca gctccgcatc gattcgactt aaattatcca
    22501 ttgatttgga taaataaccc agtaattcga aaattcccgg atattcttcc gatcttcctt
    22561 ccagttgctc catggctttt tgagtcagtt ggctgagatt ctcggcattc gccagccgat
    22621 cccgatcttt cttcagcgtt tcttcttccg attcattgat tttggccgag cgaatctcat
    22681 tcatttgaaa ggacaacata tctttttttc gcagcaattc ttcgtcattc atgcgaatct
    22741 cggtcaatcg ggtacgaatt ttctgtaatt ttttgaattc cgcttgatat ttcttcagca
    22801 attcctgatt tccggcaaag cgatccagca agacgatatg attggaagga ttcagcagtg
    22861 acagatgatc ggattgaccg tgaatatcca ccagcagccg gccgatttcc cgcagcagat
    22921 taacgttcac actgctgccg ttgacccgtg ccgagctgcg gccgccggcc cgaatatcgc
    22981 ggctcaagag gatctgatcg ccgtccgaat cttcgggcaa atcttccctt ttcagtattt
    23041 cccgaatcgc ctcccgattc tcgtccggcc ggaaaaccgc ttcgacaatc gcccgatccg
    23101 tcccttcacg gataaaagtc ggatcggtct tcccgccgac aaccgttgaa acggcatcca
    23161 aaataatcga tttaccggcg ccggtctcac ccgtgaagat attcaaacct tccttcagag
    23221 aaagttccag ctcatcgata atcgcaaaat ttcggatata taattcggtc aacatttcag
    23281 cgtctgcgaa tggcaaaacc gcgcatattc gttaatatgg tcgtacataa caaggcaatg
    23341 tacaaaattc cccaaccgac ggacagggta tatcccatca aaccggcgta atttccgatg
    23401 aatatcatcc ggaaaagccc caagatatat cgcagaatcg gaaccgctcc gccgattccg
    23461 gccgcgattg ccgtcaattt attcagatcc gccgctcggc gtttttgagt aaccgcccgg
    23521 acggctttgg cgatcagatt ccccaacaac aatcccagaa tgatcgccgt cagcatggaa
    23581 taaaaattaa aaaaatacaa tacaaaagat cccaaggctc cgatcagaaa agcaacgatc
    23641 gccgataaaa cataatcccg ggatacggcg gtgttgaatt ttttctgctg ctctttgaca
    23701 cattccttac agcgataacc ggtcggtgtg gagaccgcgc aatccatgca aataggccgc
    23761 ccgcattgat tacaacaaat caatgtctcc cgattcggat gcttgtaaca ggttttaatt
    23821 tcgcttattt catcactcat atcgttgtct ttcgattggc gctcggattt cgctgcatat
    23881 attgaataaa attcgagtag aaaaatccct ttccttccaa gcgcacataa cgcgccgaaa
    23941 attctgaagc ggagaccgaa atcgtatccg attcatcaat catcatccca tcttgcccgt
    24001 ccggtgaaaa aatcgtctcg tgttcggaag acgatcgaat ttccaccaca tcgttttcgt
    24061 cgagaacaat cccgcgctcc aggcacaaat ggggcgccac gggaatcacc agaatatttc
    24121 gcaattccgg ttccataatc ggtcccccgg ctgccatggc gtatgcggtg gaccccgttg
    24181 cagtggaaac aatcaatccg tccgcgacat aatccgtcag ataattttca ttaatgcgga
    24241 cgttgatacg aatcgggcgt aatttggaac catgaccgat caccgcatca ttcagcactt
    24301 cccatcgagt tcgttcgtta ttctttcgaa ccagctcgac atgcaacatc atccgttttt
    24361 cgatccgcca atccccgttc attaaagccg gaagtttttc ccgccattga tttttggcga
    24421 tttccatcat aaaaccgaag ctgcccatat tgatcccgca gaccggaacc tcaaatggtg
    24481 cgcatagttt attggtgcgt aacatcgtcc cgtcaccgcc gaaagtaatc accaaatcaa
    24541 aggggcggcg ttcaaggctt tcacgaaaag cttcatcgta aagggaccaa ccttccgcga
    24601 cagcctgtcc gtgaaaacgg gtattgattt cttcgataat cttcttggct tcttcttcgg
    24661 cggcagccag atgagggtgc caggtcacac cgattcgttc gggttttttt gtcgtttgat
    24721 tcatcttcgc tcacaatttt tcatcacaaa tcgatcggta tccgcaaccc ccgcagcgga
    24781 atgaatcgca atgagaacga tgaacttctt cgagcgaatc atcttccctg atcctttcca
    24841 accggtccag aagttcatcc cttcttttta ttgtatacga aatcttaaac tctttgtttc
    24901 gataacgcaa aattccgtaa tccggcttca cggcaaaggc cttctccgcc aacaggcaat
    24961 aagccattaa ttggaagacg tgagaagccg gcggtgtttc cggagccggc tgagatttga
    25021 tttcgatcgg aatcagttgt tccccttttt gaatcaacag atccggtttc cccgccaagc
    25081 gggtttccgg gtcaaacaga ggggctttca acttcccgtt cggagcgata tcctcataaa
    25141 ccggtttgcc ttgaaagaga acatcggaag ctttctttcg ctccgtcaaa atcaacccgg
    25201 ccagcgcaag caccaaaagc aaaattccga ggagcagctg ctgattcatc tcgcccccac
    25261 gttcgccgca atccaccaag aaatcaagcc cagcgccgaa atcagcatta ggatcaggat
    25321 aatccgggca atccgcatcg ccgataatcg cctgcccatt ttccgatgaa aacgggtgcc
    25381 ttcattcatg gcgggcaggt tttcgttggg agcgtttcga ttccgatacc accatgcccg
    25441 cgcacaatag acataggtcc ccagctcgga agcggtgata attttttgcg aggttttatt
    25501 tttcattttc aatcgatttt accgcagccg gcccgatact cccgcgaaac attcgcagaa
    25561 aaagtcgaac agccgatccg tttcggcgcg ctgagactgt ttttcttcgt ctccgtacgt
    25621 cgggaacggc gcggcgaaag aagcaagccc gatcgcaccg gtcctcttgc ggaggcgttt
    25681 ccgcggcaca atcggaaaaa cctgcaccga atttctcaac cccggtgcag gatatccgtg
    25741 aacgatatgc cgttttattt tttttcgaaa acttcgatcg ctttttgcag ttccgctttc
    25801 agcttttctt cccgcaagct caaagtcaaa tccgctcgcg tccgttcaac gataccgcgc
    25861 aaaagatccg tctgccgccg caatccgttt gtcacggcat cgggataatc catcgtgatc
    25921 agaatcgaat aaatctcatc catcaggctc agcaatcgtt ccgcttcttc cgagtatccc
    25981 attcgcagga tatccataca gcggcggcgc aattccccgg gcgtctcggc catcccgcgc
    26041 agataagtgg cggtcagcac ccccatctct tccgcagtgg gaatcggctc gtttttgata
    26101 aacgcacagg tcagcgtcgc ttcgcaatgt tccttgatgg catcctgagt gtaaccggca
    26161 taatacagat ccggatatat ttccagctcg gcttttaatg cctcgaacag cttttgccct
    26221 tcctgtaatt ggctgtgcgc ctcatccgct tccccgcgat ggatggcgcg aatggcattc
    26281 gccgaatgcc gcgtcaacga acgtgctttc gttagagcat tatcccgtgc cagcgtacgg
    26341 gcttcaaaat cctgataagc ccgaccggct atttccgaaa ttttatcgat gtagttcatt
    26401 agaacggaat ctcatcctca tcgatttccg aatcgctgta ttcggggact ccgccgctcg
    26461 catttccggc attttccgat tcggaacggg tcgtcaacga acggatcgtc gaggcattca
    26521 cttcgaagga agccgaagcg gtcccgtcgt tgcgcgtcca aacccgcgga cccccggtat
    26581 tgggatccgg aaccagccgc ccttcgatca acaccttatt gccttttttc agatagttat
    26641 ttgcatattc cgccaatttt gaccagcaag tcaccctgaa ccaaattgtc tcctttaccg
    26701 tctgaccctg agtattcgaa tattgtcggc tggaagccac actgaaagac gtaaccggaa
    26761 cccctgacgg tgtgtatcgc atttccggat cccggccgat attccctgct atgataatcg
    26821 tatgaaacat gttttctcct tattcaattg tcttctatcg gttttccatt gttgtggaca
    26881 attgtatcaa aataactttt ttctgtttag gaaattcatt tcccgagatc cgttttcatc
    26941 cgcaaatccc gcgaaaagtg atttttaaac ataaaaggcc gggaatcaat cccgaccttt
    27001 acatgaaaag gagtgccgtc tttcaatttt tcaatctgag acggcttgga aaaagataaa
    27061 aaaagaagaa ttttgctttt gctgtcccga tcttgattat gttacaaatg tcatgatttg
    27121 tcaatgccta aatatgacat tcgtcaaatt tgatgacatt tcttttttca ttcattcgac
    27181 cgtcacggat ttcgccaaat ttcgcggttt atccacatcg cagccacgca ataccgccgt
    27241 gtaataggcg aacagttgga gtggaacgat agccaccaac ggcgcaaaaa aatcatccac
    27301 tttgggaatc cggatgatct catccgccaa tccctccggc accgtgatat cctcttgggt
    27361 caccagcagc acccgcgcac cgcgggtttt cacctcgcgg atattgccga acatcttctc
    27421 gatcagggca tattgcgtcg ccaccgccac cacgctgacg ccgtcctgaa tcagtgagat
    27481 cgtgccatgc ttcaattcac cggcggcata tgcctcgctg tggatataag atatctcctt
    27541 taatttcagc gatccttcga ccgagacggc gttatccagc gaacggccga tgaaaaaggc
    27601 atgatccgca ttcatatact cgctggcaat gtgcttaatt ccatccgtat ccgatagaac
    27661 cttctcgtcc agcgccggga cggaaaacag cgcggcggtc agctcaagct ctcgctgcgg
    27721 tgatatctgc ccgcgaatcg ccgcgatttt gatcgccaac agatagagca gccccaactg
    27781 taccgaatac gctttcgtcg aagccaccgc aatctccgga cccgcccagc aataaacggt
    27841 tttatccgcc tcgcgggcga tcgtcgaacc caccacattg acgatcgcga tggttgtgca
    27901 acccgcctgc ttcgccaacc gcatcgccgc aatggtatcc aacgtctctc ccgattggga
    27961 aatgacgatc accggatcac cctgcgccaa aatcgggtcg gcataacgaa actcagaggc
    28021 gatatccacc gtcaccggca aatgaatcag tttttccatt gcgtactttg ccacgatacc
    28081 ggcatgcatg gccgtcccgc agccgatgat gtgaatgcac tgtgcctccc ggaagaccgc
    28141 gtcgtcgatt ccttcgccgc tgaaatccgg cctcccttcg cgaatgcggg gagccagtgt
    28201 cttttccagc gcggaaggct gttcatgaat ttccttcagc atgaaatggg gataaccgcc
    28261 cttctcggcc gactcgatgt cccaatcggc ggttaatatc tcttttttca cccgatttcc
    28321 cagtgaattg aaaatatcca ccccgtcctt ggtgatcacg gcgatatcgt ccgtatccaa
    28381 caagctgtac cggcgggtat atttcagaaa agccggaatg tccgaagcga cgaaattctc
    28441 tccgtctccg tatccgatca ccagcgggct ttcccgccga acggcgtaaa tgcgatccgg
    28501 aaaatccaag aataaaatcg ccagagcgta ataaccgtgc aattcctgcg tggttcgaag
    28561 aatcgtccga atcgggtcgc cgtcgtaata ataatccagc agcttgaccg cggcttccgt
    28621 atccgtctcc gacaaaaatt cataaccctt ttcgatcaga aaatcgcgga tttcagcatg
    28681 attctcgata attccgttat ggacgatcga aacccgggcg ctggaatgcg gatgagaatt
    28741 aacgtcgctc ggttctccgt gggttgccca gcgggtatgt ccgatcccgc agcatccggt
    28801 cagcggttcc atcgaagcca gcttctctcg cagacaagat aaacgaccct tggcttttac
    28861 cgtccgaatt tgattctgtt cgataactgc cacccccgcg gaatcatacc cgcgatattc
    28921 aagcctggtt agaccgtcca acagcaccgg caaacattct ttggatccgg catatccgac
    28981 aattccgcac attttttcat cctttccgac ccgttgtcag aaacatcaaa aacagaagaa
    29041 tcttaatcca ttttcaagat cccgaaagat tttcctgacc ccgctcatcg gcgccggaac
    29101 ggcttccccc gcaacttttt catgtcacga gcaataaagt cccccgttat acataaaaaa
    29161 gacgacctcc ccttacggag aagtcgtctt cgtcctaatc caaaagaaaa attttagaga
    29221 aaaccctgca ccagagccag cagaacaccg ccggcgatca ccgaacccag ctgaccggcc
    29281 gtgttggcac ccatagcgtg catcaaaata aagttatccg gatcttcttc caaagctatc
    29341 tttgccgcga tccgtcccgc catcgggaac gcgctgattc cgcaggcacc gatcaacggg
    29401 ttgatcttcc gaccggtaat taccttcatg aactggccga agagcatacc gaagaccgta
    29461 tcgagcacga aagccagcag ccccagtacc aggatgaaca gaacaccgac cgtcaggaac
    29521 tggctgccga tcatggttcc gccgaccgcc aggccgagga acagcgtgga aacgttgata
    29581 atttcatttt gagcggcttt atccaaacga tccacaacac cgctttcctt cagcaggtta
    29641 cccagcatca gagcggcgat caacggcgca gccgccggag aaataatgct gataatgatc
    29701 gtaaccagca tcgggaaaac cacgatcgaa cgatcggaaa cttcgcgcgg tgcatattcc
    29761 atgcggatct tccggtcttc tttgctggat aacgccttca tgatgggagg ctggataatc
    29821 ggaatcaagc tcatatagga ataagccgcc acggcaatcg gtcccagcaa attgggagcg
    29881 atcttcgatg tgacatagat agccgtcggc ccgtcaatgg cgccgatcgt cccgatggat
    29941 gcggcttccg caagccccca atggagaccc gggaaagatt tagcaaggaa ggaagccagt
    30001 aaaagcgtcc cgtaaattcc gaactggccg gcagcgccca gaataaccat cttcggcatt
    30061 gccagcagcg ggcgaaaatc gatcatcgcg cccacgccga caaaaatcag cagcgggaac
    30121 aattccgtcg taatcccggc gtcatacaac accttcagga aacccgagcg aaccacttcc
    30181 gtcgcctggc cgtttaccac ttccgtcacc gtaaatgtct gataagccga cataccgagg
    30241 ttcgccaaaa cgcagccgaa accgatcggc agcagcaacg tcggttcata ttccttgaag
    30301 attgccagcc cgatcaaaac caaaccgaca ataatcatca caatctctct ccacgagaga
    30361 agataaaaag tatctacgat actaccgagg ttccccatat cgatacctcg ttaatctgcg
    30421 aatgacagta aaacctgccc gtgcgtcaca gaatcgcctt tatggacgtt gatcgtcgca
    30481 acggttccgt cgcgattggc aaggatgtta ttcttcattt tcatcgcttc caaaacgaac
    30541 aggacatccc ctttcttcac agtttgaccc tctttgacca aaacctcctg gatcacaccc
    30601 ggaatcgggg ctttgacggt atcggagccg gcagccgcag gcttcggagc gggggcagcg
    30661 gcgggttttg ctgcgtgagc gacgggagcg ggtttggcag cggaatcccc gtaaaccttg
    30721 aattcggtcc cgtcgacttt cacagtcatc gggttggcat ttggatcggc aatttcgaca
    30781 tcgaatgttt tatcttcgac cttaacagta agtttcattg gtaagactcc tttaaaaatt
    30841 ttttatcgtt cagtctttta attcttcgaa aaacaattat gattccttct tggcggcaac
    30901 ataaccggca gcggcggcgg caacctgcat cttcagatcg taattctgaa tggaagccac
    30961 agcggcggcg gcagcctgca tcttcaacac attctcatcc accatttcaa cgacgggggt
    31021 gatgatttcc gcttcgactt cttcgatcac ttccgctttt tcgggatctt tgatcaccct
    31081 gacaatgatt tccattacgc cccaaaggat caataatgaa ataaacagga tcagaatccc
    31141 gatcagcgta atctgacccg cctgacccat cacatgggaa ttgtcgatca acttttcagt
    31201 ggtttcaact atttcaactt ccatctgttt tttcctttcc tgccggcttt tctataccgg
    31261 catatttccg tgctttcgtt cttccggcac gatttttttc ctttttattc cggacaaagc
    31321 ttttataatc tttttgcgtg tttcctgcgg ttcgatcaca tcatcgataa agccggtttc
    31381 cgccggcaca aagggattca aatatttctt tcgatattct tcgaccaagc ggcgatattc
    31441 cgcgtccgga tcctgtgccg cttcgatctg ttttcgatac aatatcttgg ctgccccttc
    31501 cggacccatc accgcgattt cagcctgcgg ccaggcgaaa gacacgtccg agcccatcat
    31561 tttgctgccc agcacgatat aggcgccgcc gtacgccttg cgggtcacga ccgtcacctt
    31621 gggcacggtg gattcggcgt aagcatacag caccttcgcc ccgtgacgga tcaccccgtg
    31681 atgctcctga tagacaccgg gcaaaaatcc cggacaatcc accagcgtca cgatcgggat
    31741 atcataacaa tcgcaggttc ggacaaagcg ggagatttta tccgatgaat tgatatccat
    31801 caccccggcc attatcgaag gttcctgggc cacaattccc accgctgtcc cgttgaaacg
    31861 ggcaaatccg ataatgcagt tcggtgcaaa ttccgcctgc aattcgaaga aactgtcccg
    31921 atctgtgatc gtttcgacga tttgatgcat cgaatacggt tcggcgggat cagccgggac
    31981 gatcgaattc aaaaagggat ccatgcggtc gatcgggtca tccgccggaa gatcggcgct
    32041 gataccgtgc caacgatccg gaagataggc cagcaagcgc cggcaatatt ccaacgcctc
    32101 tcgatccgaa tcggctgcca gatgcgccag accgctttcc gcgagatgaa cctcgcttcc
    32161 gcccaacgtc tcggagtcga tctgctcgcc ggtcaccgat tcgaccacat ccggcccggt
    32221 gagaaacata aacgattttt tccgggtcat aatgataaaa tcggtcgtcg cgggtgaata
    32281 ggccgcgccg ccggcacagg gtccgagtat caaactgatt tggggaacca cgcccgatag
    32341 acccgtggtg cgcataaaga tgccggcata tccggcaagg ctgacgatgc cttcctgaat
    32401 gcgcgcgccg ccggaatcga tcagatgaat caccggcgct ttgttttcct ttgccatatc
    32461 catcagatga cagattttcc ggctttgaat ctcgctcatc gtcccgccgt tcacggtgaa
    32521 atcctgagca tataaataga ccgtgcggtt atcgatcgtg ccgaagccgg tgatcacgcc
    32581 gtctccgtaa tatttgtttt tggtatcaaa acgggaaaga gtataaggtt ccaattccag
    32641 aaaagaatcc ggatccaaca gggtttcaat gcgctcacgg gcggtcattt ttccctgagc
    32701 atggtgtttt gcgacccttt cctttccgcc gccgtccaga atctgttgtc tgcgccggcg
    32761 aagttcttca tattcagaat cagacatcaa attttccatt ctaccaattt taccttactt
    32821 cctccgtttt gccgttcccg atcggaagaa gtgttatcct cgcgggcgtt ccgccccgag
    32881 gctggcatcg aaaggatgtc cgattgagta tataattact cataatctat cgtaccggat
    32941 tcttgccgtc acagcagggt gcgg
//
LOCUS       DAOSMM010000020        32430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1407_length_32430_cov_16.752680, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000020 DAOSMM010000000
VERSION     DAOSMM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 32430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32430
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1407_length_32430_cov_16.752680"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..990)
                     /locus_tag="PK898_04715"
     CDS             complement(181..990)
                     /locus_tag="PK898_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU19370.1"
                     /translation="MSNKKIFLIIYNQLFLIFGMLCLGINVIRCSAEPVRKILPYKSA
                     MINYSEPIKFSIYLPESNDQTDKYPVLYLLHGQSQDEKIWDQMGIKEIADHLISTGSI
                     SPLVIVMPREEAYMEKISESDFGNRIFNELIPYVESNFPVLTDPASRAIGGISRGALW
                     AQKIAFNHYGTFGLLGQHSLPAAVFSDYVIYRIYADSEGKIPLMKIRIDSGTEDPYLK
                     GALAFSKQLADVKAPYTLVVEPGGHDPEYWKEHLEDYLIWYADNLKNPAND"
     gene            complement(980..2284)
                     /locus_tag="PK898_04720"
     CDS             complement(980..2284)
                     /locus_tag="PK898_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU19371.1"
                     /translation="MLYSFVCPFFTDFDSISEEEFIDIWTNGVPIAGKVSSIAMTEET
                     FGRLSIKFGNVHSALIKIRSQKEIAERLERIKSTECAIVPTNDLQPNWKRISIGEQAS
                     APWDQDYDVHRDLLAVSGEYSELDFDPEKITTVLLTGTTALARTTAYKMIEKGITYPG
                     EKIRDIFENSEIRHISNESSFWSVCPTPILASNGKMQFCTPASFIDLFDYLGVNVVEL
                     TGNHLRDYDWPPLSETFQILKDHHIRYYGAGKNKEDAAEVLFIEESGNKFAFLGCNIA
                     GPEHVFADENLPGVNRCDMDTLEKQVSELHAAGYLVIVTLQYYEAYSRTPTEQQEKDF
                     QRISDAGAVVVSGSQAHFSQTMKPFDDRFIHYGLGNLFFDQMDRPVVGTREEFLDRYV
                     FYDGKLIQVELITALLEDYAQPVPMKADERQKFLKEIFSYVK"
     gene            complement(2547..3803)
                     /locus_tag="PK898_04725"
     CDS             complement(2547..3803)
                     /locus_tag="PK898_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU19372.1"
                     /translation="MSKEKFYESLKNEVKRIDESKTSKRFENLIEDFTDDPSPRAIIN
                     GKKFAIFNSNDYLGFRHHPALKKAEHDATERLGIGPGAVRFISGSFKIHRQLEKKLAE
                     FHGRDDAIVFSSAFATNLAVIFSLVRGQSKDSLINSNTLVISDELNHRSIIDGVRLAN
                     LGKDEKLIFRHRDTGSLSQQLEANIGKFDRVVVISDGVFSMLGAYQDLKKVREVMDRY
                     DSKYPEGIFLIVDDCHGIAAFGKTGRGCEEVSGAKADALVGTLGKGFGVDGGYVVSDQ
                     VVIDYLRESAATYIYSNSITPGCAAAALKAVEILDSDEGRKMLANLERNISLLKTELK
                     KKGFTLAADSSHPIQPVLIGDPGTTKKIIDEMYSRGYLLSSINYPVVPKGRDEIRVQL
                     SAAHTEQDIIDFVNAFTAGAKAVGVL"
     gene            complement(3796..4740)
                     /locus_tag="PK898_04730"
     CDS             complement(3796..4740)
                     /locus_tag="PK898_04730"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU19373.1"
                     /translation="MKRILVTGACGLVGSDLVLALQSKFGSENIIALDLHDPPKEFQG
                     RFEKMDVRDFEKLKSLILQYEIGQIFHLAGLLSAGSEKNPDMAWDVNLSGLKNVLDIA
                     KDHNIRVFWPSSIGVYGETTPKILTPQHTSFEPNTMYGVTKVSGELLCQYYHHRYGVD
                     VRSLRYPGLNGYKAMPGDGTTEYAIHIFYGLIRENRYECFLSADTRLPMMYMDDAIRG
                     TLMLMDAPSEAITIRTSYNFTAISFTPAELVAEIRKISPDFKIIYKPDYRQKIADSWS
                     QSIDDSQARKDWGWKEEYDLPKMTTALYEGIKERISNE"
     gene            5010..6050
                     /locus_tag="PK898_04735"
     CDS             5010..6050
                     /locus_tag="PK898_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOU19374.1"
                     /translation="MIRFNSDYTEGAHPAILERLAETNFVQTNGYGEDEYCFQAKQTI
                     KKVCNKENADVHFLVGGSQTNLTVISSALRPYQSVLSPVSGHIFVHETGAIEATGHKV
                     EALPSTDGKITASQIEEKYLSHIHDESFEHMTQPKMVYISNSTELGTIYNRAELKEIS
                     DVCRQYDLYLYMDGARLGYALTCDENDLDLPFIAECCDVFYIGGTKQGALFGEAVVIT
                     RDELKKDFRYMIKQKGGMLAKGRLLGLQFQVLFEDNLYFQLARHANETAAFLRKSISG
                     AGYSFLIPSPTNQQFPILPNDLLAELQKKYVYSNSQQIDKNHSAVRLCTSWATKWEDV
                     KQLAQDIEGYNE"
     gene            6102..8048
                     /locus_tag="PK898_04740"
     CDS             6102..8048
                     /locus_tag="PK898_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU19375.1"
                     /translation="MKQKIRAISLFIIFILFFSSLTAFAQDTESATADIVFTHDLHSY
                     IDPVQVNENGVLVEKGGFARIKTIIDSVKKQNENTFVLDGGDFSMGTLYQSVFSEKAL
                     ELRLLGLMGYQATTIGNHEFDYRSDGLAKMLDAAKASNDPLPIIVQSNINWEASQGEY
                     TEMLHKSMENYGVKKYAIIQAGDLKAAVFGLMGKEAASYAPTSGLIFDDIVESSKEIV
                     NEIKKNENVDLIIALSHSGTNSDPKSSEDEILAKEVPEIDVIISGHSHTYMDNPILIG
                     DTAVVSAGEYGRFVGNLKLSKKENNRWKVDQFTPIPVDESIEPDPDLQKTIDHYRESL
                     NSYIQLFGFDSYDQIIARNPYPFDSVQALYKDPVDNPLGNLISDSYVYEIKKVEGENY
                     IPVDVAIVPVGIIRSTLNQGDIHVRDAYEILSLGIGEDGISGYPLINVYLTGAELRMV
                     AEIDASLSDLMSGTKLYWSGLYSEFNPHRMILNRVVDVKLIGEDGSLKDLDDKKLYRV
                     VAGLYSGQMLGAVESMSKGLLQLKPKDKDGNPLEGFSSVIIKNQSGMEVKEWYAFADY
                     LSSFEKTDGISTIPAKYSKSEGRKVINDSTGIKDILRNPSAPFLKVAGILLLAILIIV
                     LIIILLVKLIRRITRKQSNKIAEK"
     gene            8122..8502
                     /locus_tag="PK898_04745"
     CDS             8122..8502
                     /locus_tag="PK898_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOU19376.1"
                     /translation="MEQKFLYCKHCGKVLIPGVDRDTPTICCGEEMVILKANSTDAAQ
                     EKHVPVVKFTDNKIEVAVGSVLHPMTPEHFIQFIYLQTEKGGQLKKLNPGDEPKAVFL
                     IGEDKPLVVYEFCNLHGLWKADVK"
     gene            complement(8601..8849)
                     /locus_tag="PK898_04750"
     CDS             complement(8601..8849)
                     /locus_tag="PK898_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOU19377.1"
                     /translation="MGLVSWLVVGGLAGWIASKIMGKDAKMGLMANIAVGIIGALVGG
                     FVLQIFGIGGVTGINLRSVLVAILGSCILLYIMNKINR"
     gene            complement(8969..11032)
                     /gene="ligA"
                     /locus_tag="PK898_04755"
     CDS             complement(8969..11032)
                     /gene="ligA"
                     /locus_tag="PK898_04755"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOU19378.1"
                     /translation="MEESEDLRKKYLQLKQEINYHNYRYHVLDDPEISDEAFDKLLVE
                     LRKIEEMHPEWITPDSPTQRAGSAVSEKFEKVTHPVQVLSLANAFNKQDVINWLDRTE
                     KMDPRVKYADFVLEPKIDGLTVVLTYQNGLLIRGATRGDGVIGEDVTPNIRTIKSVPL
                     AIPVSEKSVKVPETIVVRGEVFILESDFEKINAELAKRGEKTWVNPRNTAAGSLRQLN
                     PEITAQRPLRIFTYQILFSSSDQPIPDTQWGTLEYLQQLGFPVSPDSRYCENREQMLN
                     HLDEWQEIRNRLDYEIDGVVIKINDLRIAGSLGFVGKDPRGAIALKFPAREATTKLLD
                     IGVNVGRTGVITPYAILSPVEIGGVVVKQATLHNFDFIRDRDIRIGDTVMIKRAGDVI
                     PYVIGPLTEKRDGTQVPYVMPDRCPSCGQPLEHLENEVAVYCVNNACPAQLVRNVEHF
                     ASREAMDINGLGIKIVEQIVQNGLIKDVGDLYSLKKEDLLSLEGFGDKKAENLITAIE
                     NSKKQPLSRLINALGIRGVGEVMAITLAEHFPSLDQLKNATADDLMQIEGIGPNVAQA
                     ILDWFSRDTNQMVLKKLQNAGVWPVNMKSAESDKAPQVLAGKTIVVTGTLSHFTRDEI
                     KDFILKHGGKPVESVSNKTSFVIVGENPGSKRDKAIELGIPLYTEQMFLDMVGIEKNG
                     QRTID"
     gene            11286..11630
                     /locus_tag="PK898_04760"
     CDS             11286..11630
                     /locus_tag="PK898_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19379.1"
                     /translation="MKIIGYIISGICIFFGVLFIWSAFGTPFSSSDLIIGLITTAIGI
                     VILYVTTKNNRKTTNIYKVDLPGEIKMNPKSCQKCGGPLNADNFTIVNGAPTVKCPYC
                     GAVYEVTEEPKW"
     gene            11649..13409
                     /locus_tag="PK898_04765"
     CDS             11649..13409
                     /locus_tag="PK898_04765"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOU19380.1"
                     /translation="MNDFFGKSKTGARLFVSVFLATLIFFIGFFAVQADYRFSIPEDL
                     VIFWVEKDGTVSVSYEFTIQNYGQDIDYIDIGMPNNNFSLRDVIAKIDDQTVDQDKIS
                     YVDPDEVGYSYGITIDNTENPIPRNAKASVYVEVQNIKNVLYLSSDSSDGTEYVSFRF
                     MPNYFGSDFTTGTTKLTVRLVLPPGMSSSDPSYFTPENWVGDDEPDSWITAEGNIVYE
                     WQSDHADSSSPYTFGAKFPKEFLNPDVKIQTESQVKNNDSGGIGSLIFIFVVGLLIII
                     PLIRSFSSSKKKKNNIVPGSYLPPSIKAEGEGIKRGLTAVEAAVLLEKRIDTIISMII
                     FSLVKKNAIQVIQQNPLKIKVSEPLPEGLYDYEKGFIEAMTSEKDSQKKAKMEKTISD
                     LINSVAEKMKGYSLAETKAYYRSIIEKAWDQVKEAGTPELKGEKLDEIFGWAILDDDV
                     DKKSQEVFGDYPVFLPTWWWRVGPVYASPTPVHPASSSGTPSPSVPSPLPAPTLPGAD
                     FARSITDTVRNFGEGTVGEIKSFTQNVIGHTNPAAAHSGSSNRRSGSGRGGSGGGFSC
                     ACACACAGCACACAGGGGGR"
     gene            13412..14779
                     /locus_tag="PK898_04770"
     CDS             13412..14779
                     /locus_tag="PK898_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU19381.1"
                     /translation="MGISNLFGFNKQFHKGLYQKKVKDESGFENRIHLRVDEDGTGVL
                     WVNANQTFYLNQSAALMTWCLINDFTDEEDRRLLKKYYPDQYEQVTKDFDAFSPALKK
                     MLRGENNVCDLCQSGIDSDMPFSANPSAPYRMDLALTYRCNNNCSHCYNDKSRQVKEL
                     PSKSWHKILEKIAQEGIPHVVFTGGEPTLYPDLIDLAEYAENLGLVAGLNTNGRKLRD
                     AELMASLVRSKLDHIQITIESDQESIHDAMVQVPGAWKETVAGIQNAVKSGIFTMTNT
                     TLLQSNGTEENVRQLINFLAELGVQTVGLNALIYSGEGKNVHTGLAESDLPALLNLAL
                     QTCENNGQQLIWYTPTQYCYFDPQDFQLGVKGCSAARYSMCVEPDGTVLPCQSWYEGV
                     GNILTDPWQKIWNHPLCNEIRERKRMPESCNICEKSQICGGGCPLAVDYKPPVKPQAS
                     IPSCF"
     gene            14797..15519
                     /locus_tag="PK898_04775"
     CDS             14797..15519
                     /locus_tag="PK898_04775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU19382.1"
                     /translation="MDTDLSALNLRFEQQAKWTFDFQRKLFHDRRTPPPWKILEVGCG
                     TGAVLRQMGQMGSVNDIFYGIDSDRVSLSFARTSEEGHFCNAFGSDLPFPNESFDLVF
                     CHYFLLWVKNPVMILKEMARVCKPGGMVVAFAEPDYEARIAYPPELDSPVHLQTDELI
                     LQGINPTAGRQLSSWMENAGFVNPVVGIHGFEWQGEQYKQFLEQEWGQLKKDIHLTEP
                     SELPIRENVIFYTPTFYAYTRL"
     gene            15748..16905
                     /locus_tag="PK898_04780"
     CDS             15748..16905
                     /locus_tag="PK898_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOU19383.1"
                     /translation="MQYDFDQIIDRRGTNSVKWEHFPADVLPLWVADTDFAVEPAIIR
                     RIQKRLEHPILGYSIDSVELKETVQQRMERLYNWKIGIESISLVPGIVSGFNFAERAI
                     CEAGDNVIFQVPAYPPFFEAPINNNLGSVLNPLVFNKDKNIYEIDFDDFEKKINPRTK
                     MLLLCNPQNPTGRVFTPAELTRLGEICLKHDLIICSDEIHSDIVYNGYRHTPIASLNK
                     ELENRSITFIAPSKTFNIPGLSCSVAIIPNPEIHKKYLKALNGFCSGVTCLSQEAGIA
                     AYQEGAEWLSQMKIYLEGNRDFLLNFISREMPSIKTTEIQATFLAWLDCSQTGIPGDP
                     QKFFEENAKVGLNEGIPFGPGYEKFVRLNFGTPRKILKEALERMANSLRQL"
     gene            16925..17596
                     /locus_tag="PK898_04785"
     CDS             16925..17596
                     /locus_tag="PK898_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOU19384.1"
                     /translation="MGSSETESNQPVIQRKKNIRKWIILLIIIAVLLGFLSLAGEYLT
                     IDSELNHADLIVVLSGNDTVRLKAAAELYKEGYSETILLTNTGRTYGEYNIPYTQLQI
                     EMLKELDVPEGAIHQVDFVAKNTGQEGTAIIEGMVNLHANSVIIVTDAWHTRRVKTIF
                     DDTFSNTNYSVQYYGVSESEINPKLWWLSADGWKNVIGEYIRIIGYYIKRDTNIPDYP
                     IFNIS"
     gene            complement(17653..17868)
                     /locus_tag="PK898_04790"
     CDS             complement(17653..17868)
                     /locus_tag="PK898_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOU19385.1"
                     /translation="MSERYVGTVKWFNATKGYGFIGRDGDSPDVFVHFSEIKMDGYKR
                     LNEGQKVEFSIEEGSRGAQATDVKVIE"
     gene            18165..18659
                     /locus_tag="PK898_04795"
     CDS             18165..18659
                     /locus_tag="PK898_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOU19386.1"
                     /translation="MTEKNNRPAKRINWKVVISLTAIIGGVLYLLISSTINNAQYFIT
                     VDELLANRQQYANKSVRVSGAVIGDTIKVSETDGSVSFEVANISADHKAIEENGGMAK
                     ALAEAVNDPSAAHLKVLYTGPKPDLLKDQAQAILTGTINNDNVFMADELLLKCPTKYE
                     GAMQ"
     gene            18661..20703
                     /locus_tag="PK898_04800"
     CDS             18661..20703
                     /locus_tag="PK898_04800"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOU19387.1"
                     /translation="MATLGYSSLVFALFAEIYGVAALMIGSVRKSKKWLSSGRQACLI
                     LFPLLSISVIALLIPLIRKDYRFEYVFETVNNAQPFYLRIAALWGKQSGSLLFWTWLF
                     SIFLFVWTLKSVKTDDPLFPTAAILLLLNIGFFNVLNLFGSNPFNRFWLNNLNGKVYT
                     AFSQISGTVSYQPADGMGMNPLLRHFGMVLHPPALYLGFIGVFIPAAYELSALIHKPD
                     HFDWIRKTHSWFLFAWIFLAIGLILGSRWAYDVLGWGGYWGWDPVEVSALIPFLLLTA
                     YIHAMIGFRKTGRYKAWVAISGLLTALSIVFSTFVTRSGLIASVHAFSASPLSQYLIL
                     YLAVSLITVVVILLIARKNFASQTVLRKLSFEQTIQTREFFITAMIVVLFVIAAFCLW
                     GILVPNISALFSEKTISIGADYYTSATGPLFLILVFLTGCFPLTSWGVPFTQKIRKIF
                     FLLLALSAVVTGWICINLAVTQPLRIISIWVLVFSISVYLFDWIDHAFVINKDHRQFS
                     FVRLFSQFSFRRPRLSTCLIHGSILLMAVGIIGIEFFQKETQFTFKPNDTRSFSGFEL
                     TFNRVTKEDTLEGYLTTAEFDIFQNGKNITTVYPKQKYYETSQQMTTIPGIKSDLKDD
                     LYLILTDSEASAEKQQVAVTIFYNPLINWLWIGGILLCLGGILAYATSLRKEKTSS"
     gene            20700..21191
                     /locus_tag="PK898_04805"
     CDS             20700..21191
                     /locus_tag="PK898_04805"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOU19388.1"
                     /translation="MNEVLFHPIPPVYDQNSRILILGTFPSPKSRESGFFYGHPQNRF
                     WKVISRVLNWNLPESINEKKEMLISNRIALWDVIASCEITGADDGSIRNPKPNPIEKI
                     LLQSEVKAIFSTGKKSFHLYRKLIYPRTAVPSNYLPSPSPANCKISLDELVRAYDAIL
                     PWL"
     gene            21316..21987
                     /locus_tag="PK898_04810"
     CDS             21316..21987
                     /locus_tag="PK898_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19389.1"
                     /translation="MNRTTDISQPILSVSGIRKKFGPRTVLKSISLDLKPGDCTVLQG
                     DNGVGKSTLLKILAGTLKPDEFDFIRINGEDSYNSPSYRKMIGSVNHQLMAYGDLDAL
                     DNLRFTAGLYGIKTNHQTILELLDRFDLSARVHDPVRTYSRGMQQRLSICKALLIRPI
                     LFLMDEPYTGLDQKSRTMLNEVILEQKSLSKAILITSHEPETLKSLATNFIKLEKGIL
                     IQENS"
     gene            21984..22658
                     /locus_tag="PK898_04815"
     CDS             21984..22658
                     /locus_tag="PK898_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOU19390.1"
                     /translation="MKSTFQTIRALIRKDMQIESHTREIFTVMLVFALSILFILNFAL
                     EQNVKVRTGSAAGLIWIILILAGQLGLNQIMGIEKQGNMIEALLASPVPRSAIYLSKT
                     ISAFVFMFLMALIIHPLYCIFYNVPMISPDFILVSALGCWGYAALGVLISAMTIQTRT
                     RDILLPVLFYPLIIPLVVTTVKVTGSILSGENFSSLLSSLYQILAFDVIFTASGILGF
                     ELIVED"
     gene            22664..23362
                     /gene="ccsA"
                     /locus_tag="PK898_04820"
     CDS             22664..23362
                     /gene="ccsA"
                     /locus_tag="PK898_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOU19391.1"
                     /translation="MNMQIPKKLKILDWFSFFLILFTCWLIFYKTPVEMNMGLVQKIF
                     YFHVAYAWIGMVSFLVAAISAIIFLRKGLFIYDAVTESAVEIGLVFTLIAIITGSIWA
                     RPIWNTWWTWDPRLTTTSIMALIYAGYLFLRGSLDNPEKRRKITSIYSIIGCLSVPLT
                     FFSIRGVRSIHPVVFGGDTPDFNMDSQMLRTMLIAIAVFSIFYIAVMWHRIRLAELKE
                     EIEQLKLTSEEDHD"
     gene            23355..23528
                     /locus_tag="PK898_04825"
     CDS             23355..23528
                     /locus_tag="PK898_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19392.1"
                     /translation="MTEIPNTLNYMIAGFLVVVIGTLIYIGSLIVRWKKYSKLKVELE
                     NLLDHSKKQQKGN"
     gene            23535..25316
                     /locus_tag="PK898_04830"
     CDS             23535..25316
                     /locus_tag="PK898_04830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19393.1"
                     /translation="MRSESSKKVLFVSGIFLIAFILRFASLGTIPLSEDEAILALQAK
                     SLASGIPNYGIAQPLYVLITAFIFKFFGPTDFTARLFPALMGIFLVSIPFFFSEKISQ
                     KKKYLFAFCLAIDPALVAWSRQADSLIPLIFLIALAIILFRRGSEKTAVALAALSFLG
                     GERFFPFLAAAGIIFALIYWINRYFLKLDFKRSEETRKQVNKQNLLIFLSVLIVAGMG
                     LLAFPEGINGIGNGIINGFKINVNHDFRSVGFVPLLIAFFVYEGSAVLLFLIKYIKSI
                     SKKEKPQVIAMSCMILFAAILSIFNQGILIIPWISLPLWFFSTDVILSLRPIKKTEID
                     LPMAIAILIPPILSFFLLLRLSEMLRMGDLSLPLQFTWQNQAVSTPFSRLPAYLLIIV
                     ICIIIFAVLLPMLLDYFSPGKIKKGMLYGCLSVLLVCTFGSSWKAAGFSNTKDDPFLH
                     TSASRHELILGNQIVQIENQIIPIIQEIGWKATGFIHQASGIILVDNDPMLRWTLQYY
                     PDVVFTDVLDIRTNSPSFVITEGSEYEPLWASYVGLVSDWKTTDHWEMYRTNDWLSWI
                     IYRKPVISGQKLVIWQQSRFLTKTNLE"
     gene            25335..26006
                     /gene="cmk"
                     /locus_tag="PK898_04835"
     CDS             25335..26006
                     /gene="cmk"
                     /locus_tag="PK898_04835"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOU19394.1"
                     /translation="MKKPLQIAIDGPAASGKSTVGKKIAENLNYLFFDTGLMYRVATY
                     AVLEQQCPLDDEGKLADIISRADIRLQNNSQTGETDVLLDGKTITDLLHLPQVDANVS
                     LIAAIPAVRKLLTAQQRKIALQGKIVIAGRDIGTVVLPEAPLKIFLLASAEKRAERRY
                     LENLRNGIPSDYDEILYNIKQRDQIDSNRNVAPLKPAEDAILIDTDSLTQNQVTDKIM
                     SLIDQ"
     gene            26046..>26327
                     /locus_tag="PK898_04840"
     CDS             26046..>26327
                     /locus_tag="PK898_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19395.1"
                     /translation="MDQKTDNKIKTILLTVQILKPATIRLILNENAGQMENEELKTWF
                     RGHNSTSLQEADVQFYQIMKNQSRIVIPSINQSRLLAGLEMEDSGAIILP"
     assembly_gap    26328..26427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26428..27589
                     /locus_tag="PK898_04845"
     CDS             <26428..27589
                     /locus_tag="PK898_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU19396.1"
                     /translation="SRLLAGLEMEDSGAIILPVRTDFGYCGCFWGCFPEASFDDTLIE
                     TFANFNDWVIEVIGNTQKNDFGVEAIAKRYADFLDQQKTPALIVIYPDQISFSNPSFE
                     AMSTKENVLQMIRKNISVDSSFMELVDLYHCRIRDVNFPGGKTGKIFQFVSATENSVP
                     VSFDANELEYFSLLVEKAGGNLELLDKAGNMSLIQFNYKEKAEAQLKRLNTLIRYEQK
                     HYKNFLNSREGSFEIINIGDLVKDVVFDLISVAKKKNLDINYDSEKVDNSQTQSGKVI
                     GDPWLLSLSIFNLLSNAIRYSKPDSKSIQVDLTYQVDSWILRIQDFGIGISPLDVEKI
                     LSDTPDRSPAQDTQAVHGLDFVKYVVKLHRGRFNLESKLGKGSTFTLDIPYY"
     gene            27791..32023
                     /gene="rpoC"
                     /locus_tag="PK898_04850"
     CDS             27791..32023
                     /gene="rpoC"
                     /locus_tag="PK898_04850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU19397.1"
                     /translation="METKGLVALGISLASPETIRSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPTRDWQCYCGKYKNPRYKGIICDKCGVEVTRSSVRRERLGHIELATPIAH
                     IWYTRRIPSYLGLLLDISRRNLDRVLYFAQYIVTYVDEDARQKALNRLEDEISVSEKE
                     VAQEVNQKILGVKANRDKMLAQIQEQRDALLAKTDEMIAQKLDPVMKEGQRLEAELQE
                     KIGQVINSDLVFSSTGTAIASAGETISNQHISLVQKTVKEKLDEIENDFKGQLQRDIE
                     RLDMDKQQVRAEAEEQMEALRNQLADESAESQNQNSKMRDELQELQPLTFMSETSYRE
                     LKSRWGNVFRADMGAEAFYDILKRLDLDQLSKDLWNEVRTTKSKQKRKKATSRLKVVE
                     SFRSSKNHPEWMILTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPKLKLYQCGLPKTMALELFHPFLVSRLVEHGYATNVKGANR
                     IIERNRPEIWEVLEEVISERPVLLNRAPTLHRLGIQAFEPILIEGNAIQLHPLVCTAF
                     NADFDGDQMAVHVPLSEKAVEEARTLMLASKNLLKPADGEPIISPSKDMVLGIYYLTM
                     DSDGDHPGDGRAFYSIDEVQYAYDLDQVKLHTKIKFLTDTWFGKDGARLLKPEKRIID
                     TTIGRVIFNNILPEEIRFKNSPLDKGAIKDLIAEVYEICGQDVTTEVADSIKNTGFEF
                     AMRSGSTIAVSDITIPDTKDALLAEADNEVESINRQWRRGLLTEQERNDRTIDVWQET
                     TNKIASEVRKSMDPQGNMAIMATSGATKGGFAPIAQLAGMRGLMADPSGRIIRLPIKS
                     NFREGLDALEYFISTHGARKGLADTALRTADAGYLTRRLVDIAQDIIINEEDCGTEEG
                     IWIRRSDDVAKQAFHERIYSRVLAEKVIDPETGEILAEKGEIIDHDLAHKISDTKSIP
                     EIKVRSPMTCELKHGICAKCYGIDLGRGKMVELGAAVGTVAAQSIGEPGTQLTLRTFH
                     SGGVAQGGDITTGLPRVEELFEARRTPKGEAVITQIEGYAKIIQSDKYNDLRIVRVEH
                     SEMNSDVFEITEGWTIKVKDEQTVNLGDIIAENGEATITAAHPGQVRIEEDKVIVSYE
                     KKESEEYEIPSTARLVIKDNQYVTSGEPLTEGSLNPHTILKLKGRDACQMYILSEIQR
                     VYRAQGQNINDKHFEVIIRKMLGKVQVVRSGDSPFLPMDIVDRLRIKEINKKLEEEGK
                     QPAKYVEILLGVTKASLSTDSWLSASSFQHTIKVLASAAIGGMKDPLYGLKENVIIGK
                     LIPAGTGFVPGRFDHEDEIVSPSALISNLPTE"
     gene            complement(32090..>32430)
                     /locus_tag="PK898_04855"
     CDS             complement(32090..>32430)
                     /locus_tag="PK898_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19398.1"
                     /translation="IFTNLVDFDMKYGHRRDVIGYGNALIEFDRWLGSFLPKMKDSDA
                     LFITADHGCDPTHEGTDHTREYVPLLIYGKGIEPAVNYGTRKTFADAGQTIASIFRVE
                     PVEDGVNLFA"
BASE COUNT         9239 a         6836 c         6870 g         9385 t
ORIGIN      
        1 catttctcgt ttttccgaat tgccgttcat ttatccggaa atttcggaag ctgatctaaa
       61 tccggtgttc ttgttgcatg aaggtttggt cgtcggagac gtacgaatta ttaaaaagtg
      121 atttttttat attttgttga tgaaaaagct tgagagattt ctcaagcttt tttgttcgtc
      181 tcaatcgttt gccggatttt tcaaattatc tgcataccaa atcagataat cctccaaatg
      241 ttctttccaa tattccggat catgccctcc cggttcaaca acaagcgtat aaggagcttt
      301 aacatcggct aattgcttgg aaaaagcgag cgctcccttg agatatggat cttcggttcc
      361 gctgtcgatt cgtattttca ttaaggggat ctttccttcg gaatcggcat agattcgata
      421 tattacataa tccgagaaaa cagctgccgg taaactgtgt tgtcctaaaa gcccgaaggt
      481 accgtaatga ttaaacgcaa tcttttgcgc ccataaagcc ccgcgtgata tccccccgat
      541 tgctcttgaa gccggatcgg taagcaccgg aaaattggat tccacatagg ggattagctc
      601 attgaagatc cggttcccaa agtccgattc ggaaattttt tccatataag cttcttcacg
      661 cggcataaca attaccaacg gagatatgct tccggtcgaa attaaatgat cggcgatttc
      721 tttaattccc atttggtccc atattttttc atcttggctt tgtccatgga ggagataaag
      781 gaccggatac ttatccgttt gatcattcga ttcgggcaaa tagatagaaa atttaatcgg
      841 ctcggaatag ttgatcatcg cactcttata gggaagtatt tttcgaaccg gttcagccga
      901 acaacgaata acattaatac ccaggcaaag cattccgaat attaagaata attgattata
      961 aataataaga aatattttct tatttgacat atgaaaaaat ctcttttaaa aacttctgtc
     1021 tttcatcagc cttcatcgga acaggttgtg cataatcttc cagcaatgcg gtgattaatt
     1081 caacttggat caattttccg tcatagaaaa catatcgatc taaaaattcc tccctggtcc
     1141 cgactaccgg ccggtccatt tgatcaaaaa acaaatttcc cagaccgtag tgtataaacc
     1201 gatcgtcaaa cggtttcata gtctgtgaaa agtgtgcctg agatccggag acaacgacgg
     1261 cacctgcatc ggaaatgcgt tgaaaatctt tctcttgttg ttcggtagga gtccgggagt
     1321 aggcttcgta atattgaagt gttactatga ccaaatatcc cgctgcatgc aattcgctga
     1381 cttgcttttc caatgtgtcc atatcgcagc gatttacacc gggtaaattt tcatccgcaa
     1441 aaacatgctc cggtccggca atattacaac cgagaaaagc gaatttattt ccgctttctt
     1501 cgatgaaaag aacctcagcc gcatcttcct tatttttgcc ggcgccgtaa taacgaatat
     1561 gatgatcttt taaaatctga aaagtttctg aaagaggggg ccaatcataa tcccgcagat
     1621 gatttcctgt caactcaaca acattaactc ccaaataatc aaaaagatca atgaatgatg
     1681 ccggtgtaca gaattgcatt ttcccgtttg atgctaatat cggcgtcgga caaacagacc
     1741 aaaaagaaga ttcattcgaa atatgtcgaa tttcggaatt ttcaaaaata tctcggattt
     1801 tttcaccggg gtaggtaatt cctttttcga tcattttata tgccgtagtt cgcgctaaag
     1861 cagttgtccc ggtcaataga acggtcgtta ttttttccgg atcaaaatcc aattcagagt
     1921 attcaccgga aacggcaagc aaatctcgat ggacatcata atcctgatcc catggggctg
     1981 atgcttgttc cccgattgaa attcgtttcc aattgggctg aagatcattt gtcgggacaa
     2041 tggcacattc ggtgcttttt attcgttcca atctttcggc gatttctttt tgagaccgaa
     2101 tttttattag tgcgctgtgc acgttgccga attttattga taatctgccg aacgtctcct
     2161 cagtcatcgc aatcgaggat acttttccgg caatcggtac tccgttagtc caaatatcga
     2221 taaattcctc ttcggatatg gaatcaaaat ctgtaaaaaa gggacataca aatgaataca
     2281 acatctcatt ttcggcatcg gcagatattg aagggactaa ataaatcaaa attaaaatga
     2341 ttaactcaag agctttcctg tttattattt cttttcctga aaaaataatc ttttttcctg
     2401 atttggattt catcaagtag ttttttcgca ataaaacacc tctctattcc gatgaattat
     2461 attttttgac aacagaaaaa tcattgaatt gcttatctca atgatttttc tgttttatgc
     2521 cagatgaaat aggtatcttc ggtcaattac aaaacaccga ccgcctttgc tcccgcagta
     2581 aatgcattca caaaatcgat aatgtcctgt tcggtatgag ccgcactaag ttgtacgcga
     2641 atttcatcgc gacccttcgg tacgaccgga taattaatac tggaaaggag atatccccgg
     2701 ctatacattt cgtcgattat ttttttggtc gttccggggt caccgatcaa aacaggttga
     2761 atcggatgag acgaatcggc ggcgagagta aatccttttt tctttaattc ggttttcagc
     2821 aatgaaatat ttcgttccag attagcgagc attttccgtc cctcatccga atccaaaatt
     2881 tccaccgcct tcagagccgc tgcggcacat ccgggagtta tcgagttgga ataaatataa
     2941 gtagccgctg attcacgtaa ataatcgatg acaacctgat ccgaaacgac atagccgccg
     3001 tcaaccccga atcctttccc caaagttccc accaaagcat cggcttttgc gccgctgact
     3061 tcctcacatc ctctgcctgt ttttccgaaa gcggctatgc catggcaatc atccacaatc
     3121 agaaaaattc cttcgggata ttttgaatca tatcggtcca taacttcacg gacttttttc
     3181 agatcctgat atgcacccaa catgctgaaa actccgtcag aaatcacgac gactcgatcg
     3241 aactttccga tattggcttc aagttgctgt gacaaagaac cggtatcgcg atgtctaaaa
     3301 atgagctttt catctttccc caaattcgca agtcgaacac catcgataat actgcggtga
     3361 tttaattcat ccgaaataac aagcgtgttg ctattaatca gagaatcttt gctttgtcct
     3421 cttacaaggc tgaagataac tgcgaggttc gtcgcgaaag cggaagagaa cacaatcgca
     3481 tcatctctcc cgtgaaattc ggctaatttc ttttcaagtt gacgatgaat tttaaaagaa
     3541 ccggaaataa accgaacagc accggggccg atacctaacc tctcggttgc gtcatgttcc
     3601 gctttcttta acgccggatg atggcgaaac cccaaatagt cattggaatt aaatatggca
     3661 aacttttttc cattaataat cgctcgcgga gacggatcat cggtaaaatc ttcaatcaaa
     3721 ttttcaaatc gtttggaagt cttggattca tctattcgtt tcacttcatt ttttaaagat
     3781 tcataaaatt tttctttact catttgatat cctttcttta attccttcat ataatgcggt
     3841 ggtcattttg ggcagatcat actcttcctt ccaaccccaa tcttttcgag cttgagaatc
     3901 atcgatcgat tgagaccagc tgtccgctat cttctgccgg taatccggct tgtaaataat
     3961 tttgaaatcc ggtgagattt ttcggatttc agctaccaat tcggcaggtg taaagctgat
     4021 cgccgtgaaa ttgtaactgg tacgaatcgt aatcgcttcc gaaggagcgt ccatcaacat
     4081 taaagttccc cggatcgcat catccatata catcatcggt aaacgggtat cggcagataa
     4141 aaagcattca tatcgatttt ctcgaatcaa tccataaaag atatggattg catattcggt
     4201 agtcccgtct cccggcatag ccttatatcc gttcaaaccc ggataacgaa gactgcgtac
     4261 atcaactcca tagcgatgat gataatattg acaaagtaat tctccggaaa ccttggtgac
     4321 accgtacatg gtattcggtt cgaaactggt atgctgcggc gtcagaattt tcggcgtcgt
     4381 ctcgccgtat acaccgatag aactcggcca aaaaactctg atattatgat cctttgcaat
     4441 atctaagaca ttttttaatc cgctcagatt aacgtcccaa gccatgtccg gatttttttc
     4501 acttccggcg gaaagtaatc ccgctaaatg aaatatttgc ccgatttcgt attgaaggat
     4561 cagagatttc aacttttcga aatctcgcac atccattttc tcgaatcgcc cttgaaattc
     4621 tttgggggga tcatgtaaat ccaaagcgat aatattttca gaaccgaatt ttgattgtag
     4681 cgccagaacc agatcactgc caactaaccc acacgcaccc gtaacgagta tccgtttcat
     4741 gttgatcctt tgtgaaagag agtggccttc tgtcgaaggt ttttaaaacc aaacctgcat
     4801 tcatgaacat taagattcta cctgttttat ccgattttca acagatttga tcatcctgtt
     4861 ttttgtgtta tttccggttg taatgcagct atccggtatt ttcaccgatt catactcaga
     4921 aaaaaatcgg tttcgatcgt tttttcgaag aaaaagaata gaatatattt cagttgttac
     4981 catgtaaata aaaaaggaat atagacagta tgattcgatt taatagtgac tacaccgaag
     5041 gcgctcatcc cgctattctc gagcggcttg ccgagacaaa tttcgttcag acaaatggtt
     5101 atggcgaaga tgaatattgt tttcaagcga aacagacaat taaaaaagtc tgtaataagg
     5161 aaaatgccga tgttcatttt ttggtcggag gatcccaaac aaatttaaca gttatttctt
     5221 ctgcattacg cccgtatcag agcgtattat cccctgtttc cggacatatt ttcgttcatg
     5281 aaacaggagc cattgaagcc accggacata aagtggaagc gcttccctca accgacggaa
     5341 aaattacggc ttcgcaaatc gaagaaaaat atttaagcca tattcatgat gaatcttttg
     5401 aacatatgac tcagccgaaa atggtctata tctcgaactc caccgaattg ggaaccattt
     5461 acaaccgcgc cgaattaaaa gaaatcagcg atgtctgccg gcaatatgat ctgtatctct
     5521 atatggatgg agccaggtta ggttatgcct tgacatgcga tgaaaacgat ctcgatcttc
     5581 cgtttatcgc cgaatgttgt gatgtttttt atatcggcgg aaccaaacaa ggcgctctgt
     5641 ttggagaagc ggttgtaatt acccgtgatg aattaaaaaa agacttccga tacatgatta
     5701 agcaaaaagg cggtatgctg gctaaaggac gtttactcgg attacagttt caggtattgt
     5761 ttgaagacaa tctttatttt caattagccc gtcatgcaaa tgaaacggct gctttcttaa
     5821 gaaaatccat ttccggtgca ggatattctt ttctgatacc ttctccgacg aatcaacaat
     5881 tcccgattct cccgaatgat cttctcgctg aattacagaa aaaatatgtt tattcgaatt
     5941 cgcaacagat agataaaaat cacagtgccg tccgtctttg caccagttgg gcgacaaaat
     6001 gggaagatgt gaaacaactc gctcaggata tagaaggtta taatgagtaa gaaataattg
     6061 ttattttaaa acttcacaat cgtttttcgg aggagagcaa aatgaaacag aaaatcagag
     6121 cgatttcgtt attcattatt ttcatcttat tcttttcttc cctcaccgct ttcgcacaag
     6181 atacagagtc ggctactgca gatatcgtct ttacacatga tttgcattcc tatatcgatc
     6241 ctgtgcaagt gaatgagaac ggagtactcg ttgaaaaggg tggttttgct cgcattaaaa
     6301 caattattga ttcggttaaa aagcaaaatg aaaatacctt cgttttggat ggcggcgatt
     6361 tttcgatggg caccctttac caatctgttt tttccgagaa agcgttggaa ttacgattat
     6421 tggggctgat ggggtatcaa gcaactacca tcgggaatca tgaatttgat tatcgcagtg
     6481 acgggctggc aaaaatgctg gatgctgcca aagccagtaa tgatcctttg ccgatcatcg
     6541 ttcaatccaa tatcaattgg gaagcttctc agggtgaata taccgaaatg ctgcataaat
     6601 caatggagaa ttacggtgtt aaaaaatacg cgatcattca agccggtgat ctgaaagccg
     6661 cagtgtttgg tttgatgggt aaagaagccg ccagttacgc accgaccagc ggcttaattt
     6721 tcgatgatat tgtcgaaagt tccaaggaaa ttgtcaatga aattaagaaa aatgaaaatg
     6781 tggatttgat tattgccttg tcccactccg gaacgaattc cgatcccaag agttcggaag
     6841 atgaaattct tgccaaagaa gttcctgaaa ttgatgtaat catcagcgga cattcacata
     6901 cctatatgga caatccgatt ctcatcgggg acaccgctgt tgtttcagcc ggtgaatatg
     6961 gtcgatttgt cggaaatctt aaactttcca aaaaggaaaa caatcgctgg aaagttgatc
     7021 aatttactcc tattccggtc gatgaaagta ttgaacccga tcctgacctg caaaaaacaa
     7081 tcgatcatta ccgtgagtca ttgaattcat atatccaact ttttggattt gacagttatg
     7141 accaaataat cgccagaaat ccttacccgt ttgattcggt gcaagcgtta tacaaagatc
     7201 ccgttgataa ccctctcgga aatttgattt ccgattccta tgtttatgaa ataaagaaag
     7261 ttgaaggaga aaattatatc ccggttgatg ttgcgatcgt tccggtcgga attattcgtt
     7321 cgacattaaa tcaaggagat atccacgttc gagatgctta tgagatatta tcactgggaa
     7381 taggggaaga cggtatttcc ggatacccgc tgatcaacgt ttacctgacc ggcgcagagc
     7441 ttcgaatggt agcggaaatc gatgcatcgc tctctgatct gatgagtggt accaaacttt
     7501 attggagcgg cctttacagc gaattcaatc cccaccggat gatattgaat cgagtagtgg
     7561 atgttaaatt aatcggagaa gacggttcac tgaaagattt ggatgacaag aaactttatc
     7621 gtgtggtcgc cggattgtat tccggtcaaa tgttgggagc cgtcgaatcc atgtcaaaag
     7681 gtttattgca gctaaaaccg aaagataaag acggtaatcc tttggaaggt ttcagttccg
     7741 taatcattaa aaatcaatcg ggaatggaag taaaggaatg gtatgctttt gccgattatt
     7801 tgagttcctt tgaaaaaaca gatgggattt cgacaattcc cgccaaatat tccaagagcg
     7861 aaggtcgaaa agttatcaat gacagtaccg gtatcaagga tattttacga aatccgagcg
     7921 ctccgttcct gaaagtcgca gggattttgc tgctcgccat tctcatcatt gttttaatca
     7981 tcattctctt agtaaaactg attcggagaa taacccgcaa acagtcaaat aaaatcgcag
     8041 aaaagtaaga tcggaaattt ttgatatact taatattggt tgaattttat ataccaaatc
     8101 atttcgtttg aggagggaaa tatggaacaa aaattcttat attgtaaaca ttgtggaaaa
     8161 gttcttatcc cgggagttga ccgagatact ccgacaatat gctgcgggga agaaatggtg
     8221 attctgaaag cgaacagtac cgatgctgct caagaaaaac atgtccccgt tgtcaagttc
     8281 acagataata aaatcgaagt cgccgtcgga agtgttttac atccgatgac tcccgaacat
     8341 tttatccaat ttatctattt gcagacggag aaaggcggcc aactgaagaa attaaatccc
     8401 ggcgatgagc cgaaagcggt tttcctcatc ggtgaagata agccgttggt tgtttatgaa
     8461 ttctgcaacc ttcatggtct atggaaagct gacgtcaaat aattcttttc atattgaatc
     8521 aatcttaaaa aaaaagcacc gctccgaatg gaagcggtgc tttttaatag cagaagtctt
     8581 ttccattgaa aagataaaat ctatctgttt attttgttca taatataaag caatatacaa
     8641 gaccccaaaa tcgctaccaa aacacttctc agattaatgc ccgttacacc gccgattcca
     8701 aaaatttgca gaacaaatcc accgactaat gcgccgataa ttcctactgc aatattagcc
     8761 atcaatccca ttttggcatc tttccccata atcttactgg cgatccaacc ggctaagcca
     8821 ccgacaacca accatgaaac aagtcccatc gttcctcctc tttcgttgaa ttaaatatac
     8881 tcggcacgat tccgtataat ttgtaattta tcgtaacaca atttattcga tcaaatgatt
     8941 atttttttat atgatatttc aaaaagtatc agtcaatcgt cctttgacca tttttctcta
     9001 ttccgaccat atcgagaaac atctgctcgg tatacagcgg tatccccaat tcgattgctt
     9061 tatcccgttt cgatccggga ttttcgccga caatcacgaa agaagtttta ttgctgacac
     9121 tttcaaccgg ttttcctccg tgtttcaaaa taaaatcttt tatttcatcg cgagtaaaat
     9181 gagataaagt tcctgttacg acaattgttt ttcccgctaa aacttgtgga gccttatcgg
     9241 attcagcgga tttcatattg acaggccata cacctgcgtt ctgtaatttt tttaaaacca
     9301 tctgatttgt atcacgcgaa aaccaatcca agatggcttg agcgacattc ggaccgatcc
     9361 cttcgatttg catcagatca tcggctgtgg cattttttaa ttgatctaat gaaggaaaat
     9421 gttcagccaa tgtaattgcc atcacttctc cgactcctcg aattcccaat gcattgatta
     9481 atcgtgacaa aggttgtttt tttgaatttt caatcgccgt gatcagattt tccgctttct
     9541 tatctccgaa tccttcgagg gaaagcaaat cttctttttt taggctgtag agatcgccca
     9601 catcttttat caatccgttt tgcactatct gttcgacaat ctttatgccc aatccgttga
     9661 tgtccattgc ttcgcgtgaa gcaaaatgct ctacatttcg caccaattga gccggacagg
     9721 cattattgac acaataaacg gcaacttcat tttcaagatg ttccaaaggt tgtccgcagg
     9781 aaggacatcg atcaggcata acataaggaa cctgagtccc gtcccttttt tccgtcagag
     9841 gtccgatcac atagggtatc acatcgcccg ctcgttttat catcaccgta tctccgattc
     9901 ggatatcccg atctcgaata aaatcaaaat tatgcaatgt cgcttgcttt acaacaacac
     9961 cgcctatctc taccggagat aaaatcgcat agggagtgat cactcctgtt cgtccgacat
    10021 ttacaccgat atccaacagt ttggtcgtcg cttcccgtgc cggaaatttc aatgcgatgg
    10081 caccccgcgg atcttttccc acaaagccca aactgccggc tatgcgcaaa tcgttgattt
    10141 ttatgacgac accgtctatt tcataatcga gtcgatttcg aatctcttgc cattcatcca
    10201 agtgattcaa catttgttcc cgattctcac aataccttga atcggggctg acggggaatc
    10261 ccagttgttg caaatattcg agtgttcccc attgtgtgtc gggaatcggt tgatcggaag
    10321 aggaaaaaag aatttgatag gtaaaaattc gtaaaggtcg ttgagcggta atttcagggt
    10381 tcagctggcg tagcgatccg gcggccgtgt ttcttggatt cacccaggtc ttttcgcctc
    10441 ttttagccaa ttcggcatta attttctcga aatcggattc cagaataaaa acttctccgc
    10501 gtaccacgat ggtctcggga acttttacac ttttttcgga aaccggaatt gctaagggga
    10561 cgctctttat cgttcgaata ttgggagtga catcctcacc gataacgccg tctccgcggg
    10621 tagctcccct tattaaaagt ccattttgat atgtcaaaac aactgtcaaa ccgtcaattt
    10681 tcggttccaa gacaaaatcg gcatatttta ctctcggatc catcttttct gttcgatcca
    10741 gccagttgat aacgtcttgc ttgttaaacg catttgccag actcaacacc tgaacaggat
    10801 gagtcacttt ttcaaatttc tcagaaacag cacttccggc acgttgcgta ggggaatccg
    10861 gagttatcca ttcgggatgc atctcctcta ttttccttaa ttcgaccagc agtttatcga
    10921 acgcctcatc ggaaatttca ggatcatcca aaacatgata acgatagttg tgataattaa
    10981 tttcttgttt caattgaaga tattttttcc gtaaatcttc gctttcttcc atacccctcc
    11041 gctacatcga actgaagctt caatattgat gatttttttc agaaagagat taatgccctt
    11101 ttctatcgtt gattaaaacc ctgattcttt aattcgataa aaaggttttt ttccattata
    11161 taatatatgt gaagaggaat tgccattaag tttcagtaga caacatgatt tttaatcaac
    11221 tcattcgtta tttctgatct tttgtgcaaa tcggacaata aaaaaggctt aaaaccggag
    11281 agaaaatgaa aattatcgga tatatcattt cagggatatg tatttttttt ggagtccttt
    11341 tcatatggag cgcatttgga acccctttca gcagcagtga tttgataatc ggtctgatta
    11401 ctacggcgat cggtatcgtt attctatatg taaccacaaa aaacaatcga aaaacaacga
    11461 acatctacaa agtagatctc cccggcgaga tcaagatgaa tccaaagagt tgtcaaaaat
    11521 gcggcggacc tttaaatgcc gacaatttta cgatagtcaa tggggcgccg acggttaaat
    11581 gtccctattg cggtgctgtc tacgaagtca cggaagagcc gaaatggtaa tcggggcgga
    11641 gaataacaat gaatgatttt ttcggtaaga gtaaaacagg cgccagatta tttgtctctg
    11701 tttttttggc aactttgatc ttctttatcg gattttttgc ggtccaagcg gattatcgtt
    11761 tttcaatacc ggaggatctc gtaattttct gggtggaaaa agacgggacg gtatctgttt
    11821 cttatgaatt tacgattcaa aactacggtc aggatatcga ttatattgat atcggtatgc
    11881 ccaataataa tttcagtctg agagatgtaa ttgcgaagat tgatgatcaa acggtggatc
    11941 aagataaaat ctcttatgtc gaccccgacg aagtcggata ttcgtacggc attaccatag
    12001 ataataccga aaatccgatc ccgagaaatg caaaagccag tgtttatgtc gaagttcaaa
    12061 atataaaaaa tgttctatat ctttcatcgg attcaagtga cggtacggaa tatgtcagtt
    12121 tccgttttat gcccaattat ttcggttcgg atttcactac cggtacaaca aaattaactg
    12181 tcaggttggt attgccgccc ggtatgagtt cttcggatcc ttcttatttc acaccggaga
    12241 attgggtcgg agatgatgag cccgattcat ggattacagc agaaggaaat atcgtatacg
    12301 agtggcaatc cgatcatgcg gacagcagct cgccctatac attcggagca aagtttccta
    12361 aagaattcct taatccggat gtaaaaatcc aaaccgaatc ccaagtaaaa aacaacgatt
    12421 cggggggaat tggctccctg atattcattt ttgtcgtcgg tttgttgatc atcattccgt
    12481 tgattcgcag tttttcttct tctaagaaga agaaaaataa tattgttccg ggcagttatc
    12541 tccctccgtc cataaaagcg gaaggagaag ggattaaacg cggattgact gccgtggaag
    12601 ctgccgtcct actcgaaaag aggatcgata caattatttc gatgatcatt ttctcattgg
    12661 tcaaaaagaa tgcgatccag gtgattcaac aaaatccttt aaaaataaaa gtttcggaac
    12721 cgttgcccga aggtctttac gattatgaaa agggttttat cgaggcgatg accagcgaaa
    12781 aagattctca gaaaaaagcg aagatggaaa aaacgatttc ggacttgatt aattccgtgg
    12841 ctgaaaaaat gaaaggctat agtttggcag agactaaagc ttactatcgg tctattatcg
    12901 aaaaagcttg ggaccaagta aaagaagccg gaacgcccga attaaaaggt gaaaaattgg
    12961 acgaaatctt tggctgggca attcttgacg atgatgttga taaaaaatca caagaggtgt
    13021 tcggtgatta tccggttttc ctgccgacat ggtggtggcg tgtcggaccg gtatacgctt
    13081 ctcccacgcc cgttcatccc gcttcttcgt ccggaacgcc gagcccttct gttccgtctc
    13141 cattaccggc ccccactttg cccggagctg attttgccag aagtatcacg gacacggtca
    13201 gaaacttcgg agaaggaacc gttggtgaaa ttaaatcgtt tactcaaaat gtaatcggtc
    13261 atacaaaccc ggcagccgcc cattcgggat caagtaatcg gaggagtggt tccggaagag
    13321 gaggaagcgg gggcggtttc agttgtgctt gcgcatgcgc ctgtgccggt tgtgcctgtg
    13381 cttgtgccgg tggcggagga ggaagataaa gatgggtatt tcgaatctct tcggcttcaa
    13441 caaacaattc cacaaagggt tatatcagaa aaaggtaaag gacgagagcg gctttgaaaa
    13501 caggatccat ttaagagtgg acgaagacgg caccggtgta ctctgggtaa atgcaaatca
    13561 gactttttat ttgaaccaga gcgctgcctt aatgacatgg tgtcttatca atgattttac
    13621 ggacgaggaa gaccgccgcc tgctgaaaaa atattacccc gatcaatatg aacaggtgac
    13681 caaggatttt gatgcttttt cacccgcctt aaagaaaatg ctacggggtg aaaataacgt
    13741 ctgtgatctt tgtcaaagcg gaatcgacag cgatatgcct ttttccgcaa acccttccgc
    13801 tccatatcgg atggatttag ctctcaccta ccggtgtaac aataactgtt cacattgtta
    13861 taacgacaaa tccagacaag tcaaagaact cccttcaaaa tcttggcata aaattcttga
    13921 aaagatcgca caagaaggaa ttccgcatgt tgtttttacc ggcggcgaac cgacgcttta
    13981 tccggatttg attgatctgg ctgagtatgc cgaaaatctg ggtttggtag ccggattgaa
    14041 tacaaacggg agaaagctga gagatgccga attgatggca tctcttgtaa gaagcaaact
    14101 ggatcatatt caaattacca ttgaatcgga ccaagaatcc atccatgatg cgatggtgca
    14161 ggtcccgggt gcttggaaag aaaccgtagc cggaattcaa aatgcagtaa aatccggcat
    14221 ttttaccatg acaaatacta ccttgcttca atcaaacgga acggaagaaa atgttcggca
    14281 attaattaat tttcttgcgg aattaggcgt acaaaccgtc ggattgaacg ctttaattta
    14341 ttccggcgaa gggaaaaatg tccatacagg ccttgcggaa tcggatttac cggcactgct
    14401 aaatttagct cttcaaactt gtgagaataa cggacagcaa ctgatctggt atacacccac
    14461 tcagtactgt tattttgatc cccaagattt ccaattaggc gttaaaggtt gttctgctgc
    14521 aagatacagt atgtgtgtcg aaccggatgg cactgtcctc ccctgccaat catggtatga
    14581 aggggtcgga aacattttga cggatccatg gcaaaaaatt tggaatcatc cgttatgcaa
    14641 cgaaattcgt gaacggaaga gaatgcccga aagctgcaat atctgcgaga aatcacaaat
    14701 ctgtggagga ggttgtccgt tggcggtgga ttacaagcct ccggttaaac cccaggcttc
    14761 cattcccagt tgcttctgat tccgaaaata tttgaaatgg ataccgattt gtctgcctta
    14821 aatctccgtt tcgaacaaca ggcgaaatgg acttttgatt tccagcgaaa attgtttcat
    14881 gaccggcgga caccgccgcc ctggaaaatt ctggaagtcg gctgcgggac cggagccgtc
    14941 cttcgacaaa tgggacaaat gggatccgta aatgatatct tttacggtat cgattcggac
    15001 agagtgtcat tgtcttttgc tcgcacatcg gaagaagggc atttttgcaa cgcattcggc
    15061 tcggatcttc cgttcccaaa tgaatctttt gatctggtat tttgtcacta tttcctgcta
    15121 tgggtaaaaa atcccgtaat gattctgaaa gaaatggcac gcgtttgtaa acccggcgga
    15181 atggtcgtgg cttttgccga gccggattat gaagcgagaa tcgcttatcc tcctgaactc
    15241 gattcaccgg ttcatctgca gacagatgaa ttaattctac aaggcatcaa tccgacggca
    15301 ggcaggcagc tttcttcttg gatggaaaat gccggctttg taaaccccgt cgtcgggatc
    15361 cacgggtttg aatggcaagg ggagcaatat aagcaattcc tcgaacaaga atggggtcag
    15421 ctgaaaaagg acattcatct cactgaacca tccgaacttc ccatccgaga aaatgttatt
    15481 ttttataccc cgacatttta tgcttatacg aggctgtaag ttccgaaaaa tcccggggcg
    15541 atattctatc gttccgaaat ccgttttatt gaaacaaccc taaaattttt tgcggttcat
    15601 catcattctt actcacgatt ttctgatcga tcaaaggttg ttttgtttta ttcggaaaaa
    15661 agaattagcc ggcaagagaa ttcttatttc ttgtttggtt tataatgctt tcggtatatt
    15721 atcataaatt ttcaggagag aagaacaatg caatatgatt ttgatcaaat catcgatcgt
    15781 cgtggaacga attcggtaaa atgggaacat tttcctgccg atgttctgcc gttatgggtg
    15841 gctgatactg attttgcggt tgaacccgca attatcagaa ggattcaaaa aagattggaa
    15901 catccgattt taggctacag tatcgacagt gtcgaactca aagaaaccgt tcaacagcgg
    15961 atggaaagac tatacaactg gaaaatcggt attgagtcta tctcacttgt tccgggtatt
    16021 gtatccggat ttaactttgc cgaaagagct atttgtgaag ccggagataa tgttatcttt
    16081 caggtccctg cctacccgcc tttttttgag gctcccataa acaacaatct gggaagtgtc
    16141 ttgaatcctt tggtcttcaa caaggataag aatatctacg aaatcgattt cgatgatttt
    16201 gagaaaaaaa tcaatccgcg aacaaagatg ctgcttcttt gcaatccgca aaatccgacg
    16261 ggaagggttt ttacgccggc tgagctgacc cgattgggag aaatttgcct gaaacatgac
    16321 ttgatcatct gctcggatga gattcacagt gatattgttt acaacggtta ccgtcatact
    16381 ccgatcgctt ctctgaacaa agaactcgag aatcgttcca taacctttat cgcccccagc
    16441 aaaacattta atattccggg gctctcttgt tcggtcgcga ttattccgaa tccggaaatt
    16501 cataagaaat atctcaaagc actcaacggt ttttgcagcg gggtgacctg cctttcacag
    16561 gaagccggaa tagcggctta tcaggaaggt gcggaatggc tctctcaaat gaaaatatat
    16621 ttggaaggaa accgtgattt tttgttgaat ttcatcagtc gggaaatgcc gtctataaaa
    16681 acaaccgaaa ttcaagcaac ttttttggct tggttggatt gttcgcagac gggtattccc
    16741 ggagatcctc agaaattctt cgaggaaaat gctaaagtcg gtttaaatga aggcattccg
    16801 tttggtcccg gctacgaaaa attcgtccgg ttgaatttcg gaacaccgcg gaaaattctg
    16861 aaagaagcgc tggagagaat ggcgaattcg cttcggcaac tttaatcaac acgatcaaat
    16921 aagtatgggt tcgtcagaga ccgagagcaa tcaacctgtc attcaaagga aaaagaatat
    16981 ccgaaaatgg ataatccttc tgattatcat tgccgttttg ttgggttttc tttcgcttgc
    17041 cggtgaatac ttgacgattg attcggaatt gaaccatgcc gatttgattg tcgtattaag
    17101 cggaaatgat accgttcggt taaaagccgc tgccgaactt tataaagaag ggtattccga
    17161 aaccatttta ttaaccaata ccggtcgtac ttacggagaa tataatattc cttatacaca
    17221 attgcagatc gagatgttga aagagctgga tgttccggag ggtgcgattc atcaagtcga
    17281 ttttgtcgca aaaaacaccg ggcaggaagg gaccgcaatt atcgaaggaa tggttaatct
    17341 gcatgcgaat tcggttatta ttgtcacgga tgcatggcat actcgtcgcg taaaaacgat
    17401 ttttgacgat actttttcca acacaaatta ttccgtccaa tattacggag tgtcggagtc
    17461 cgaaataaat ccgaaattat ggtggctttc tgcggacgga tggaaaaatg tcatcggaga
    17521 atatattcga atcatcggct attacatcaa aagagatacg aatattccgg attaccccat
    17581 ttttaatatt tcttgattct aaaaaaaaga gactgctcat ttccggagca gtctctaaaa
    17641 ttttccttta tcttactcga tcaccttcac atcggttgcc tgagcgccgc gcgaaccttc
    17701 ttcaattgaa aattcaactt tttgtccttc attaagccgt ttatatccgt ccatcttgat
    17761 ttccgagaaa tgaacgaaaa cgtccggaga atctccgtct cgaccgataa aaccatagcc
    17821 ttttgtagca ttaaaccact ttactgtacc tacatatctt tcactcattt tattaactcc
    17881 gattgttttc ttaattccat tttggcaacc cctccgcctt atgttttatc gtactgaaaa
    17941 gaaaaatttg agggttcttt tcaaacgtta atatttttat cactcataaa aaaaagcgtc
    18001 aaggaaaagg accgaaaatt tttgaatttt tacctgcatg ttttatagtt gactccgaaa
    18061 atctgcataa tctcccttta atcgggaaag agaaatactc aagaatgata aaattatgtc
    18121 tgtttaataa agtcttcaaa aatgccttct tttcggggga agaaatgaca gaaaaaaata
    18181 atcgcccggc aaaaagaatt aactggaaag ttgtaatcag tctgaccgca atcatcggcg
    18241 gagttcttta cctgcttatt tcctcaacaa tcaataacgc gcaatatttc attaccgtag
    18301 atgaacttct tgcaaatcgc caacagtatg cgaataagtc ggttcgagtt tcgggcgcgg
    18361 ttatcggaga caccatcaaa gtctcggaga cggatggatc cgtttcattt gaagtggcaa
    18421 atatttctgc cgaccataaa gcaatcgaag aaaacggcgg aatggcaaag gcattagccg
    18481 aagcggttaa tgatccttcc gccgctcatc tgaaagttct ttacaccggt ccgaagcctg
    18541 atttgctaaa agatcaagcc caagcaatcc tgaccggaac aatcaacaac gataatgtat
    18601 ttatggcgga tgagctctta ttaaaatgcc cgacaaaata cgaaggagcg atgcaataag
    18661 atggcaacct taggatattc ttcattagtc tttgccctgt ttgctgaaat ctacggagtc
    18721 gcggcgctga tgatcggatc cgttcggaaa tcaaaaaaat ggctgagcag cggtcgtcaa
    18781 gcttgtttga tcctttttcc gctgctcagt atttcggtta tcgctttatt gattcctttg
    18841 attcggaaag attatcgttt cgaatatgta tttgaaacgg ttaataatgc ccaacctttt
    18901 tacctccgga tagcggcttt atgggggaaa caatccggat cgctcctttt ttggacctgg
    18961 cttttttcaa ttttcttgtt cgtttggaca ttaaaaagcg tcaagacgga tgatccgcta
    19021 ttcccgacag ctgctattct gctgctctta aatatcggat tcttcaatgt tttaaatctc
    19081 tttgggagta acccttttaa tcgtttttgg ttaaacaatt taaacgggaa agtttatacc
    19141 gctttttcgc agatttccgg taccgtttct tatcaacccg ccgatggtat gggaatgaat
    19201 cccttattac ggcatttcgg tatggtactt catccgccgg cactttattt gggatttatc
    19261 ggggttttca ttccggcagc ctatgaatta agcgctttaa ttcacaaacc cgatcatttt
    19321 gattggatcc gaaaaactca ctcatggttt ctgttcgcat ggatttttct ggcaatcgga
    19381 ttgatcttag gatctcgatg ggcgtatgat gttctcggtt ggggcggata ttggggctgg
    19441 gatccggtcg aggtcagtgc cctaattcct tttttacttt tgacagctta cattcatgca
    19501 atgatcggtt tcagaaaaac aggtagatat aaggcctggg tagccatctc cggattgctg
    19561 accgcgctat caattgtttt ttccactttt gttacgcgat ccggattgat cgcttccgtt
    19621 catgcttttt cggcgagtcc tttatcacaa tacctgattt tatatttggc ggtttctttg
    19681 ataaccgtag ttgttatatt attgatcgcg cgtaaaaact ttgcctccca aaccgtctta
    19741 agaaaattat cttttgaaca gacgattcag acccgagagt tttttattac agcgatgatt
    19801 gtcgtacttt ttgtaatcgc cgcattttgt ttatggggaa ttctggtccc gaatatttcc
    19861 gcgctttttt cggaaaaaac gatttcaatt ggagcggatt attacacttc ggctaccgga
    19921 ccgttatttc tgattctggt tttcctgacg ggatgctttc cgctgacttc ttggggagtt
    19981 ccgttcactc aaaagatccg aaagattttc tttcttcttt tggccctgtc tgctgttgta
    20041 accggttgga tttgcatcaa tctggccgtt actcaaccgt tgaggatcat ttcgatttgg
    20101 gttctcgttt tttcgatatc ggtatatctc ttcgactgga tcgatcatgc ttttgttata
    20161 aataaagatc acagacaatt ttcatttgtc agattatttt cacagttttc attccgtcga
    20221 cctcgattat caacctgtct gattcacgga agtattcttc ttatggcggt aggcatcatc
    20281 gggattgaat ttttccagaa agaaactcaa tttacattca aaccgaacga tacccgctct
    20341 ttctccggat ttgaactgac attcaaccgt gtcaccaaag aagacacact cgaaggatat
    20401 ttaacaacag ccgaatttga catctttcaa aacggcaaaa atattacgac tgtctacccc
    20461 aaacagaaat attatgaaac aagtcaacag atgacaacaa ttccgggtat taaatccgat
    20521 ctaaaggatg atttatatct gattctgaca gattcggaag cttccgctga aaagcaacaa
    20581 gttgccgtca caatattcta taatccgctc ataaattggc tttggatcgg tggaattctc
    20641 ctttgcctcg gcggaatact tgcatatgca acttcgttga gaaaagaaaa aacgagttca
    20701 tgaacgaagt gctctttcac cccattccgc cggtatatga tcaaaattcg cgcattttga
    20761 ttctcgggac atttccttct ccaaagtcaa gggaatcggg ttttttctat ggacatccgc
    20821 aaaatcggtt ctggaaagta atcagccgtg ttcttaattg gaaccttccg gaatcaatta
    20881 acgaaaaaaa agaaatgctt atttcaaatc gaatcgcttt atgggacgtc atcgcatcct
    20941 gcgaaattac cggcgccgat gacggcagta tcagaaatcc caagcctaac ccgatcgaaa
    21001 aaatattact gcagagcgag gtgaaagcca tattttctac cggtaaaaaa tcttttcatc
    21061 tttatcggaa attaatttac ccaaggacgg cggtcccgtc gaattacctt ccttcaccga
    21121 gtccggcaaa ttgtaaaatc tcattggatg aattagtacg agcatatgat gcgattcttc
    21181 catggttgta aaaaggatct gaaacaatgg aatcgaaatt ctttgaatta gctgtttgga
    21241 ttattctggc tgtcggaaca agtctttttt tgatctggcc ctttttgaaa gattcgaata
    21301 aataattctc actcaatgaa tagaacgaca gatatttcac agcctatttt atccgtttcc
    21361 ggcattcgaa aaaaattcgg tccgaggacg gttttgaaat cgatttcact ggacctgaaa
    21421 ccgggtgatt gtacagttct tcagggtgat aacggagtcg gtaaaagcac tctgctgaaa
    21481 attctcgccg gaacattgaa acccgatgag ttcgatttta ttcggataaa cggagaagat
    21541 tcttataatt ctccttctta tcgaaaaatg atcggctctg ttaaccatca gctgatggca
    21601 tacggcgact tggatgcgct tgataacctg cgatttaccg cggggctgta cggtatcaaa
    21661 acaaatcatc aaacgatcct cgagcttttg gatcgatttg acttaagcgc cagagttcac
    21721 gatccggtac ggacttattc acgcgggatg cagcaacggc tttccatctg caaagctctt
    21781 ttgatccggc cgattttatt tttaatggat gaaccgtata ccgggttgga tcaaaaaagt
    21841 cgaaccatgc tgaatgaggt catacttgag cagaaatcct tatccaaagc gattctgatt
    21901 acctcccatg aaccggagac actgaagagc ctggcaacaa attttataaa acttgaaaaa
    21961 gggatcctta tacaggaaaa ttcatgaaaa gcactttcca gacgatccgg gcattaatcc
    22021 gcaaagatat gcaaatcgaa agtcatactc gggaaatatt taccgtgatg cttgttttcg
    22081 ccttatcgat ccttttcatc ttaaattttg cgcttgaaca aaatgtaaaa gttcgtaccg
    22141 gcagcgccgc cgggctcatt tggattattc tgattcttgc cggacaactc ggattaaatc
    22201 aaattatggg aatcgaaaaa caggggaata tgatcgaagc cttgttggct tcaccggtcc
    22261 cccgttccgc tatctatctg agtaaaacga tttccgcttt tgtctttatg ttcctcatgg
    22321 cattgattat tcatccgctt tattgtattt tctataatgt cccgatgatc tctcccgact
    22381 tcatcttggt ttccgctttg ggttgctggg gatatgcagc cttaggcgta ttgatttcgg
    22441 caatgacaat tcaaaccaga acaagagaca tccttttgcc cgttctgttc tatcctctga
    22501 tcattccatt ggttgtaacg accgttaagg tgaccggatc gattctttcc ggagagaatt
    22561 tttcttctct tttatcttct ttatatcaaa ttttagcatt cgatgttatt tttacggcaa
    22621 gcggcattct gggttttgaa ttaatagtgg aggactaaat aaaatgaaca tgcaaattcc
    22681 aaaaaagctc aaaattctcg attggttttc tttctttttg attcttttca cttgttggct
    22741 tattttttat aaaacgcccg tggaaatgaa catgggactc gttcaaaaaa tattctattt
    22801 ccatgtcgca tatgcttgga tcggaatggt cagctttctg gtagcggcga tcagtgcgat
    22861 tatttttttg cgaaaaggcc ttttcatcta tgatgcagtg accgaatccg ccgtcgaaat
    22921 cggactggtt ttcacattga tagcgatcat cacaggatcc atctgggcgc gacccatttg
    22981 gaatacctgg tggacatggg atccgcgctt gacgacaacc agtatcatgg cacttattta
    23041 tgccggttat ctttttttgc gcggctcgtt ggataatccc gaaaaaagga gaaaaatcac
    23101 ttccatatac agcattatcg gatgtctgag cgtgccgttg accttctttt cgattcgcgg
    23161 agtccggtcc attcatccgg tcgtattcgg aggagatacg cccgacttca atatggattc
    23221 acaaatgctt cgaacgatgt tgattgcgat agccgttttt tcaatttttt atattgcggt
    23281 catgtggcat cgaatccgct tggcggaact gaaagaagaa atcgagcaat taaaattaac
    23341 ctcggaggaa gatcatgact gaaattccca atacgctaaa ctatatgatt gccggatttc
    23401 ttgtcgttgt tatcggaaca ttgatatata tcggttcatt aatcgtaagg tggaaaaaat
    23461 atagtaagct taaggttgaa cttgaaaatc tgttagatca ttcaaagaaa caacaaaaag
    23521 gaaattaatc ggctatgcga agcgaaagca gcaaaaaagt tctttttgta agcggaattt
    23581 ttctgatcgc atttatttta cgttttgctt ccctcggaac aattcctttg tccgaggatg
    23641 aagcgatttt ggcattacaa gctaagtcac tggcttcggg aattccaaat tacggcattg
    23701 cacagcccct ttatgtttta ataaccgcat ttattttcaa attcttcggt ccgacggact
    23761 ttactgctcg tttatttccg gcgttgatgg gaatttttct tgtttcgatt cctttcttct
    23821 tttctgaaaa aataagccaa aagaagaaat acctgtttgc tttctgctta gctatcgacc
    23881 ccgctttggt tgcctggtcc cggcaagcgg attctttgat cccgctgata ttcctgattg
    23941 cattagcaat tatattattc cgaagaggtt cggaaaaaac agccgttgct ttggcagcgc
    24001 tttcttttct cggcggagaa cgctttttcc cgtttctggc agctgcggga atcatttttg
    24061 cgttgattta ttggataaat aggtattttt tgaaattgga tttcaagcga tcggaagaga
    24121 caaggaaaca agttaacaaa cagaatttat tgatttttct gtcggttctc atcgttgcgg
    24181 ggatggggct cttagctttt ccggaaggga tcaacggcat tgggaacgga ataatcaacg
    24241 gcttcaaaat caatgtcaat cacgattttc gatcggtcgg ttttgttccg ttgctgatcg
    24301 ccttttttgt ttatgaaggc tccgcggtcc tgctttttct tattaaatac atcaagagca
    24361 tttcgaaaaa agagaagcca caggttatcg caatgtcatg catgatttta ttcgctgcga
    24421 tcctttcgat ttttaatcaa gggattctga ttattccttg gattagcctt cctctatggt
    24481 ttttttcgac ggatgtcatt ttatcgctca gacccattaa gaagactgaa atcgatctgc
    24541 cgatggcgat tgcaatttta atcccgccga ttttgtcctt cttcttattg ctccgtttga
    24601 gtgaaatgct tagaatgggt gacctgtcgt taccgttaca atttacctgg caaaatcagg
    24661 cagtctctac accgtttagt cgactgccgg cttatctttt aattattgtt atttgcataa
    24721 tcatctttgc cgttttactc cctatgctgc ttgattattt cagccccggg aaaataaaaa
    24781 aaggaatgtt atacggttgt ctatcggttt tgcttgtctg tacattcgga agttcttgga
    24841 aagctgcggg attcagcaat accaaagatg atccctttct tcatacttcc gcttcaaggc
    24901 atgaattgat cttgggaaac caaattgttc aaatagagaa tcaaataatt ccgataattc
    24961 aggaaatcgg ttggaaagct accggattta ttcatcaagc atccggaatt attttggtcg
    25021 ataacgatcc gatgctgcga tggacgttgc aatattatcc tgatgtcgtt tttacagacg
    25081 ttctggatat ccgtacaaat tctccgtctt ttgtgattac cgaaggatct gagtatgaac
    25141 ctctgtgggc ttcatatgtc gggctggtat ccgattggaa gactaccgat cattgggaaa
    25201 tgtatcgaac aaatgattgg ctttcctgga tcatttatcg gaagcccgta atttccggcc
    25261 aaaaacttgt tatatggcaa caaagccgtt ttttgacaaa gacaaatctt gaatgaaaag
    25321 agacacctgc tttaatgaaa aagcctctcc aaattgctat tgacggaccg gccgcttccg
    25381 gaaaatcaac agtcgggaag aaaattgccg agaatttgaa ttaccttttt tttgacaccg
    25441 gtttgatgta tcgagtggca acttacgcgg ttctggaaca gcaatgcccg ctcgatgacg
    25501 aagggaaatt agcagacatc atttcacgag cagacatcag gttgcaaaat aactcgcaga
    25561 caggtgaaac ggatgttttg ctggacggaa agacaataac cgatcttctc catttacctc
    25621 aagtcgatgc aaatgtttct ctgattgccg ccattcccgc cgtcagaaaa ttattgaccg
    25681 ctcaacagag gaaaattgct cttcaaggca aaatcgtgat agccgggcgg gatatcggaa
    25741 ctgttgtttt acccgaagcg ccgctgaaga tttttctgct cgcttccgct gaaaaacggg
    25801 cggaacggcg atacctggaa aatctcagga acggtatccc gtcggattac gatgaaattc
    25861 tgtataatat taaacaacga gatcaaatcg attctaaccg taacgtcgca ccgcttaaac
    25921 cggctgaaga tgcgattctc atcgacaccg attcgttgac gcagaaccag gttaccgata
    25981 agataatgtc attgattgat caatagaata tataaaatag acatctcgaa gactggaaaa
    26041 ggaatttgga ccagaaaaca gataataaaa tcaaaacaat tcttttgacc gttcaaatcc
    26101 tgaagccggc caccatccgt ttaatattaa atgaaaatgc cggccaaatg gagaatgaag
    26161 agctgaaaac ctggtttcgc gggcataaca gtacttctct tcaggaagcg gatgtccaat
    26221 tctatcaaat catgaaaaac caatcccgca ttgtcattcc ctcgatcaat cagtcgcggc
    26281 tgttagccgg attggagatg gaagattcgg gcgcgattat tttgccgnnn nnnnnnnnnn
    26341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gcggctgtta gccggattgg agatggaaga
    26461 ttcgggcgcg attattttgc cggttcgaac ggatttcggt tactgcggtt gtttttgggg
    26521 atgttttccc gaagcttctt tcgatgatac cttaatcgaa acttttgcaa atttcaacga
    26581 ttgggtcatc gaagtcattg gaaatactca gaagaatgac tttggtgtgg aagcgatcgc
    26641 caaacgctat gcagattttt tggatcaaca aaaaacgcct gctttaattg tcatctatcc
    26701 ggatcaaatc agcttttcca acccttcgtt tgaagcgatg agcacaaaag aaaatgtgct
    26761 tcaaatgatc cggaaaaata tttcggtcga ttcctccttt atggaactgg tggatttgta
    26821 ccattgccgc attcgcgatg tcaactttcc cggcgggaaa acgggtaaaa tctttcaatt
    26881 cgtttcggcg acggagaatt ccgttccggt aagttttgat gcgaacgaac tggaatattt
    26941 ctcattactg gtcgagaaag ccggcgggaa cttggaattg cttgataaag ccggaaatat
    27001 gtcgttgata caatttaact ataaggaaaa agcggaagcg cagttaaaac gcctcaatac
    27061 actaattcgg tatgaacaaa agcattataa aaacttttta aattcacgcg aaggatcttt
    27121 tgaaattatc aacatcggtg acttggtaaa agatgttgtt tttgatttaa tatccgtggc
    27181 caaaaagaaa aaccttgata tcaattacga ctccgaaaaa gtagataaca gccagacgca
    27241 aagcggaaaa gttatcggag atccctggct gctttccctt tcgattttta atttattatc
    27301 caacgcgatt cgttattcca aacccgacag caaatcgatc caagtggatt tgacctatca
    27361 agtggattca tggattctgc gaattcagga tttcggaatc ggtatttctc cgttggatgt
    27421 tgaaaaaatt ctcagtgata caccggatcg atcacccgca caagataccc aggcggttca
    27481 cggtttggat ttcgtaaaat atgtagtaaa attacaccgt gggagattta atctggaaag
    27541 taaattagga aaaggttcga ctttcaccct ggatatccca tattattgag aacattcttg
    27601 tcacattttt caaaaagccg cttaaatata gcggaggaat ctttctgttg tgttataatg
    27661 ataaatcggc tttaatgcat ttaaagccga atgagctttt aataactcct tttatgcata
    27721 gagaaaaaag aagctatgca ttgaatgtct taatgaatgg gaaacactca aactctcggg
    27781 aggaaaattc gtggaaacta aaggtttggt tgcattagga atcagtctcg cttcacctga
    27841 aacaattcgg tcatggtctt acggtgaagt tttaaagccc gaaacaataa actatcgacg
    27901 tcttcgaccg gaaaaggacg gattgttttg tgaagctatt tttggcccga cgcgtgactg
    27961 gcaatgctat tgtgggaaat ataaaaatcc acgttataaa ggaatcatct gtgataaatg
    28021 cggcgttgaa gtaactcgtt cttctgttcg gcgggaacga ctcggccata tcgagttggc
    28081 taccccgatc gctcacatct ggtatacccg gagaattcct tcatacttgg gattactttt
    28141 ggacatttca agaagaaatc tggatcgtgt tctttatttt gcgcaatata tcgtcactta
    28201 tgtcgacgaa gatgcccggc agaaggcatt gaatcggctt gaggatgaaa tcagtgtgtc
    28261 ggagaaggaa gtcgctcagg aagtcaatca gaaaattctc ggtgtaaaag cgaatcgcga
    28321 taagatgctc gcccaaattc aggaacaacg ggatgctttg ttggcaaaaa cggacgagat
    28381 gattgctcaa aagctggatc cggtaatgaa agaaggccag cgcttggaag cggaattaca
    28441 ggagaaaatc ggtcaggtta tcaactcgga tttggtcttt tcatccaccg gtaccgctat
    28501 cgcttccgcc ggcgaaacaa tttccaacca gcatatttct cttgttcaaa aaacggtaaa
    28561 agaaaaattg gatgaaatcg aaaatgattt caaaggacaa ctgcaacgcg atatcgagcg
    28621 attggacatg gacaaacagc aggttcgcgc cgaagcggaa gagcagatgg aagcccttcg
    28681 caatcagctc gcggacgaat cagccgagtc gcaaaaccaa aactcgaaaa tgcgggatga
    28741 gcttcaggaa ttacaaccgt taactttcat gagtgaaact tcataccgtg aactcaaaag
    28801 ccgctggggg aatgtttttc gggcggatat gggcgcggaa gcgttttacg atattttgaa
    28861 acggttggat ttagatcaac tttcaaaaga tttatggaac gaagttcgaa caaccaagag
    28921 taaacaaaaa cgcaagaaag ctacgtcacg tctcaaagtc gtcgaatctt tccgttcttc
    28981 gaagaatcat ccggaatgga tgatcttaac cgttctgccg gtcatccctc ctgatttgcg
    29041 tccgatggtt cagctggatg gcggacgttt tgccacatcc gacctgaacg acttataccg
    29101 gcgggtcatc aaccgcaaca atcgtttgaa gcggctgttg gaattgggtg caccggatgt
    29161 catcgttcgg aacgaaaaaa gaatgttgca ggaagcggtg gattcgctga tcgataactc
    29221 acaacgcggg aaagcgttgt cacgccgcgg tcgccgcgaa ttgaaatctc tcagcgatat
    29281 gctgaagggt aagaaaggac gattccgcag aaatctgctc gggaaacggg tcgattattc
    29341 cggtcgttcg gtgattgtcg tcggaccgaa attgaagttg tatcaatgcg gtctgccaaa
    29401 aacaatggcg ctcgaattat tccatccttt cctcgtttct cgcctggtgg aacatggtta
    29461 tgccaccaat gtcaaaggtg cgaatcgtat catcgaaaga aatcgaccgg agatctggga
    29521 agttcttgag gaagtgatca gtgaaagacc tgttttattg aatcgtgcac cgacgcttca
    29581 ccgattgggt attcaagcct tcgaaccgat tttgatcgaa ggaaatgcga ttcaactgca
    29641 tccgttggtt tgtaccgctt tcaacgcgga cttcgacggt gaccaaatgg ctgttcatgt
    29701 accgctttcc gaaaaagccg ttgaagaagc gcgtacattg atgctggctt cgaaaaactt
    29761 actcaaaccg gcggacggag aaccgatcat cagcccatcc aaggatatgg tgctcggtat
    29821 ttattatttg acaatggaca gtgacgggga tcatccgggt gacggaaggg ctttttactc
    29881 tatcgatgaa gtccaatatg cttatgatct cgaccaggtc aagctccata ccaagattaa
    29941 attcctgaca gatacctggt tcggcaaaga cggcgctcgc ttattgaaac cggaaaaacg
    30001 aatcatcgat acaaccatcg gacgggttat ctttaacaat atattaccgg aagaaattcg
    30061 ctttaaaaat tcaccgttgg ataaaggcgc cattaaagat ttgatcgccg aagtatatga
    30121 aatttgcggt caggatgtca caacggaagt cgccgattcg attaagaaca ccggctttga
    30181 atttgcgatg cgctccggtt cgacgattgc cgtatcagat attacgattc ccgacacaaa
    30241 ggatgcatta ctggcggaag ccgacaacga agtcgaatcg ataaaccgcc aatggcggcg
    30301 cgggttactg acggaacagg aacgaaacga tcgcacgatc gatgtttggc aggaaaccac
    30361 caacaaaatc gcatccgagg tccgcaagag catggatcca cagggaaaca tggcgattat
    30421 ggcaacttcc ggcgcgacaa aaggcggttt tgcaccgatc gcacagttag cgggaatgcg
    30481 cgggctgatg gctgatccat ccggtcgtat tattcggctg ccgatcaaat ccaatttccg
    30541 tgaaggtttg gatgcgttgg agtacttcat ttccacccac ggagctcgga aagggctggc
    30601 ggataccgcc ctccggaccg ctgacgctgg ttatctgacc cgtcgtttgg tcgatatcgc
    30661 gcaagatatt attatcaacg aagaagattg cggaacagaa gaaggtatct ggattcggcg
    30721 ttccgatgat gtggcgaaac aggctttcca cgaacgtatt tacagccggg tactggcgga
    30781 aaaagtgatt gatccggaaa ccggcgaaat tctcgccgaa aaaggcgaaa taatcgacca
    30841 cgatctggct cataaaattt ctgatacgaa gagcataccg gaaataaagg ttcgctcacc
    30901 aatgacctgc gagttaaagc atggaatttg tgcgaaatgc tacggtatcg atttgggccg
    30961 cggaaaaatg gttgaattag gcgcggcggt cggtacggtt gccgctcaat ccatcggtga
    31021 acccggaaca caattaactc tccggacctt ccattccggc ggtgtcgcgc agggcggaga
    31081 tattacaacc ggtctgcccc gtgtcgaaga gctgtttgaa gcccgccgca ctccgaaagg
    31141 cgaagccgtt attacgcaaa tcgaaggcta cgctaagatt atccaatcgg ataaatataa
    31201 cgatctgcgg atcgttcgag tcgagcactc cgaaatgaac agtgacgtgt ttgagattac
    31261 ggaaggttgg acaatcaaag tgaaggatga gcaaaccgtc aacttgggag acattatcgc
    31321 cgaaaacggc gaagctacca tcaccgcagc tcatccggga caggttcgga tcgaagaaga
    31381 caaagtaatt gtctcttacg aaaagaagga aagcgaagaa tatgaaattc cgagcaccgc
    31441 tcgcttggtc atcaaggata atcaatatgt aacttccggt gaaccattga ccgaaggttc
    31501 gttgaatcct catacgatct tgaaactcaa ggggcgggat gcctgtcaga tgtatatttt
    31561 gtccgaaatt cagcgtgttt atcgagcgca gggacagaat atcaacgata aacactttga
    31621 agtgatcatt cgtaaaatgc tcggcaaagt gcaggtggtt cgctccggtg attcaccgtt
    31681 cctgccgatg gacattgtcg atcgcttacg gatcaaggaa atcaataaaa aactggaaga
    31741 ggaagggaaa caaccggcaa agtatgtcga aattttgctc ggcgtaacca aagcttcttt
    31801 gagcacggat tcctggctgt cggcttcttc gttccagcac accatcaaag tgctggcaag
    31861 cgcggcgatc ggtggaatga aagatccgct ttacggactg aaagaaaacg tcatcatcgg
    31921 gaagctgatt cccgccggaa ccggatttgt tccgggacga tttgatcacg aagacgaaat
    31981 cgtcagcccc agtgctctga tttccaatct gccgaccgaa tagatcattt tagaaaagaa
    32041 aaaacagctc gcaaatgact gcgagctgtt tttttatgct tattattaac tatgcgaaaa
    32101 gattcacacc gtcttctacc ggttcgacac ggaaaatact cgcaattgtc tgtcccgcat
    32161 cagcaaaagt ctttcgtgtt ccgtaattaa cagcgggttc gattcctttt ccgtaaatca
    32221 acaacggcac atattctctg gtatgatcgg tcccttcgtg ggtcggatcg caaccatgat
    32281 cggcggtaat aaacaatgcg tcggaatctt tcattttcgg gagaaaagaa cccaaccacc
    32341 gatcaaattc aatcaaagca ttcccgtaac cgatcacatc ccggcggtgt ccgtatttca
    32401 tatcaaaatc aaccagattg gtaaaaatca
//
LOCUS       DAOSMM010000021        29181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1608_length_29181_cov_19.136133, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000021 DAOSMM010000000
VERSION     DAOSMM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 29181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29181
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1608_length_29181_cov_19.136133"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..301
                     /locus_tag="PK898_04860"
     CDS             86..301
                     /locus_tag="PK898_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19399.1"
                     /translation="MLKKELLDILRCPVCVKENKGNLINYKDSWLICEDCQRKYPVVE
                     DIPVMLIEEGSKWIQTDPENLQIPPEI"
     gene            321..1862
                     /locus_tag="PK898_04865"
     CDS             321..1862
                     /locus_tag="PK898_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOU19400.1"
                     /translation="MAQTNYPQNQDSNKEYPNNRAPQNYGIPGQFPPNFNPQQPHGAI
                     PPQPQPKKKKNNKKWVILGVFALVGLAIGIFLFTKVNQYVKAMTVIDLPGAPVISDNG
                     KVAVETESNPDATEEAVSLSDITKVKGQPWDGVSRITCLAMGLDYRDWEAQEKYSRTD
                     SMMLLTYDPVTKMAGMLSLPRDLWVAIPNHGYGRINTAYYLGEAENLPGGGPQLAIDT
                     VELFLGIDIQYYAVISFEGFVNFIDSIDKLAINVKDQITVDPIGPGNTVTLYPGVQDL
                     DGATALAYARQRYTEGGDFERAQRQQDVIYALYEQMVWQLPQLLLHADKLYNTISQAI
                     RTNLTFDDIIKLAWTVVDIKPEQIVRSVIAPPYQVQYGKTYDGTQDILIPIPDKIREA
                     RDVIFSTSLAASPATVADDQATLIAAEGAHISLLNGSTNPDITDKTVAYLQQFGITID
                     SIGQAANSYTNSLVINKGKPFTAKFLADLMAIPTAAITMDYQPDAASDLVLTITDAWA
                     NSNPM"
     gene            1927..3495
                     /gene="gpmI"
                     /locus_tag="PK898_04870"
     CDS             1927..3495
                     /gene="gpmI"
                     /locus_tag="PK898_04870"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOU19401.1"
                     /translation="MEKPVVLVIMDGVGIGKGDIGDAVKKANTPTLDWLRSHCPYTQI
                     KAHGTAVGLPSDEDMGNSEVGHNALGCGQIYSQGAKLVNESIENGSIFHSETWNKLIG
                     NCVENHSTLHFIGLLSDGNVHSNINHLKAMIREAKNQKVSRVRIHILLDGRDVPSTSA
                     LQYVNDLESFLKTLNDNSFDARIASGGGRMKVTMDRYQADWNMVKIGWDTHVLGIGRQ
                     FANAAEAIETYRKELEVIDQDLPAFVIAENGKPIGTIEDNDSVIFFNFRGDRAIEISM
                     AFDYPDFNKFDRVRYPKVFYAGMLQYDGDLKLPKNFLVNPPNIQHTLTELLVSRHLSQ
                     YAISETQKFGHVTYFWNGNRSGKLSEELETFEEIPSDMVSFDERPWMKSAEITDKMLS
                     AISSGKYDFIRCNYPNGDMVGHTGNFDATVIAVEAVDLELARIVKLMESHDFTLVVTA
                     DHGNADDMIEKTKSGELQPKTSHSLNPVPFMIYDKTREWKIKEGSYGLANVTGTLVKL
                     MGLEPPEIWEKPMI"
     gene            3937..5487
                     /locus_tag="PK898_04875"
     CDS             3937..5487
                     /locus_tag="PK898_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19402.1"
                     /translation="MYKRKILFVFIFLTVFAFAAQGLLTVSAQSDNAAKAPDQDLVIA
                     FNNDIRALDPHNVSDTLTISATRTMYESLVKFNEKQEIVPLLAESYEISDDGLTYTFH
                     LRSGVLFHDGTEFNSDAVKANIDRCKDESLRQNRIVKQIANAEYPDPYTVILTLAKPN
                     NTFLNKMTMFDFIAPATIAAGEEKILREPCGTGPYTFKDRVEGDHVTVVPFEKYWDGA
                     PQVASLTFKAVPEDGARVAMLQTGEADYIYPMPTTQIKVVEGKKDILIDAKTSNIMRY
                     VTLNTELEQLKDKRVRQAINYAVDKDAYIKTVFNGYASKVYSCYPETIQYYAPQPPYD
                     FNLEKAKALMKEAGAENGFKLTIWGDNITSEEKGMQFIQQQLKQIGIDVEVMPMEANV
                     VAEKIQAERDKAEVNMWYVNWSASSFDADGAIRAILHGESIPPGQYNSAYYNNEEVNK
                     LMDEALAITDPKILQEKYARIQEIVWDDAPWIFLASDQVISGYKSYCSGIQLYPDGAI
                     DCSKAKLN"
     gene            5733..6653
                     /locus_tag="PK898_04880"
     CDS             5733..6653
                     /locus_tag="PK898_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU19403.1"
                     /translation="MGRYFVKRVLGTIPLLLVISFLIFMFIHLIPGDPVRNMAGREAT
                     TEELENLRESMGFNKPLYQQYFSYLNDLFHGDLGISYKTKLPVSEMMIGRLKPTLKLT
                     FFAMLWSTIVGIAIGVLSAINRGKLIDYLGMLIAISGISIPGFWLGLVMIQIFSVKLG
                     WVPTGGLDTWKSYFLPSITLGCGIMAILARFSRSSMLDSLGEDYVRTARAKGQRERLV
                     ILRHAFRNSLVQVVTVIGLQIGGLLAGSVLIETVFTIPGLGRLLVDSLAFRDYKVVQA
                     LLLFFSFEYILINLIVDLLYGVLNPRIRLK"
     gene            6666..7544
                     /locus_tag="PK898_04885"
     CDS             6666..7544
                     /locus_tag="PK898_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU19404.1"
                     /translation="MQTSTFIDQPIKKAENRTKYYFRKFFQRKTAVIAAVIILIFLFF
                     AIFGPQIAPFDPSLPDYDNLYGAPDAVHWFGTDEFGRDVLSRLLVGTRNSLGVSLASV
                     LIGAAIGTFLGVLAGYYGGIIDSVVMRGSDILFAMPDILLAIAIVAIIGPGIINVVIA
                     VAVFTIPSFARIMRSATLSVKSSLYVEAARSIGCKNSRILWVHIFPSTIQSMIVNFTM
                     RIGTAILAAASLSFLGYGAKVDEPDWGAMLSSGRNYLDIAPHLVYFPGLLIFIAVLAF
                     NLLGDGLRDTLDPKLK"
     gene            7556..8572
                     /locus_tag="PK898_04890"
     CDS             7556..8572
                     /locus_tag="PK898_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19405.1"
                     /translation="MSENNLLKVENLRTEFKISRKKSVAAVSDITFEINKGEIVGLVG
                     ESGCGKSVTSLSIIRLLNDTSGEITHGSVWFKGSNLLKLSDEEMRQIRGKEIAMIFQE
                     PMTSLNPVLRIDNQLIEAIQLHTEYTKQEAREHALETLRSVGIPDPERVSRCYPHQLS
                     GGMSQRVMIAMAMSCYPDLLIADEPTTALDVTIQAQILDLMQGIRNTRGTSILLITHD
                     LGIVAETCSRVYVMYAGRIIEEAPVDILFSDPSHPYTQGLIASIPKLGLEAESLPFIP
                     GSVPDITMMPGGCRFHPRCRYAMEICSQKEPELISINESQKCRCWLKTSEYSIKTENG
                     ESRK"
     gene            8569..9534
                     /locus_tag="PK898_04895"
     CDS             8569..9534
                     /locus_tag="PK898_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU19406.1"
                     /translation="MTDLLTINNLVKHFPAEKKLFGKQDYVHAVNNVSLEVREGETFS
                     VVGESGCGKSTVSRLINHLIQPDSGEIWFDGTETAHLNENEFRPFRQHMQMIFQDPNS
                     SLDPNMRIFDLISEPLLIFTEMSDRERRERVRELLEVVGLSYNHAERYPHEFSGGQRQ
                     RIGIARALSVNPKLIIADEPISSLDVSIQAQILNLLQKLQKQFNLTYFFISHDLSVVQ
                     LISDHVAVMYLGFIVEKAPKKELYQHPLHPYTQALLSAVPIPDPKIKKQRILLAGDLP
                     SNIHLPAGCPFNSRCFRCQDRCREEKPELREIEKDHFVACHFADL"
     gene            complement(9585..10841)
                     /locus_tag="PK898_04900"
     CDS             complement(9585..10841)
                     /locus_tag="PK898_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOU19407.1"
                     /translation="MSSYPKNWFLDAKYGLFIHFGLYSLLGGEYKGKVTPFFSEWIMN
                     DLNIPLSEYEQLAAQFDPFEFDADKIAESALRWGTRYLCFTAKHHDGFALFGSSCSDY
                     NSVCRTPSRRDYVSELAEACRKKGIVFCLYYSQAQDWHHPDGYVAYHDNQNKNFRRYL
                     DEKCLPQLTELLSDYGPIGMMWFDTPMGMKAEECEEIYQLVKKLQPDCLVSGRIGNGL
                     GDYFCTQDNRLPAFPLKRKWEIPGTLNRSFGYKKSDTNWDSAETVIRKLVKIVSRGGN
                     YLLNVGPDGSGKIPQESTKIMDEAGEFLSENGEAFYGAEAVPPYVYETPDIYFTHKPH
                     RLFLTVFHPGQFPELTVALPNIANIPQSAKILRGNRPIAFNYRKTLENDSYWEIFLPP
                     DAENQMVVVICVETQEADVRFELLDG"
     gene            complement(10887..11858)
                     /locus_tag="PK898_04905"
     CDS             complement(10887..11858)
                     /locus_tag="PK898_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19408.1"
                     /translation="MPDKQPLVSVKNLKKYFNVGKNLTLKAVDDVTFDIYPGETLGLV
                     GESGCGKSTLGRTLIRLYHATDGEIWFDGMNLTKDLTSRESFQFTRRAQMIFQDPYAS
                     LNQRMKVMDIIAEGIDAHHLAADKKEREEMVVELLQTVGLQREHANRYPHEFSGGQRQ
                     RIGIARALAVNPDFVIADEPISALDVSIQAQIVNLLQDLQEKRGLTYLFIAHDLSMIK
                     HISDRVGVMYLGSLVELTTADELFANSLHPYTQALLSAIPLADPNLEKSRKRIVLQGS
                     LPSPINLGSGCRFASRCPQVTEICKEVSPELIEVSPGHFVACHLTDR"
     gene            complement(11848..12855)
                     /locus_tag="PK898_04910"
     CDS             complement(11848..12855)
                     /locus_tag="PK898_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19409.1"
                     /translation="MGKILQVENLHVEIKTELGRIQAVRGVSFDLDKKETVAIVGESG
                     SGKTITVKSVMQLLPSTGSITEGSVRLEGKELTTLSEKEMLKIRGSDISMIFQDPMTS
                     LNPTMTVGNQIVEVLKNHRKDLSRDEMKRRAIEIIDLVGISNPETRFKQYPHQLSGGM
                     RQRIIIAIALACDPKVLIADEPTTALDVTIQAQILDLMKELQEKINTSIIIITHNLGV
                     VANIADRVYVMYGGKIVENGSVEDIFYHPQHPYTKGLLAAVPKLHEKTDKLFTILGTP
                     PDLTDPPKGCPFAARCSYTLKVCRQYPPAFTPFENHHESACWLHDPRAKRMINEVRNA
                     G"
     gene            complement(12864..13790)
                     /locus_tag="PK898_04915"
     CDS             complement(12864..13790)
                     /locus_tag="PK898_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19410.1"
                     /translation="MENYRDLPLELFEKAEVNLDEKEHIAKPSLTAFQDGWIRLKKNK
                     AAVVSLFILILIFLFAVFGPIFSSYDYAKTDYHHTFQLPSREHIFGTDQFGRDQFARI
                     WYGTRISLLIAFAAACLDIFVGVTYGAVSALAGGKVDAVMQRIIEILVGIPSLIIIIL
                     LMMVMPAGIGTIIIAMSITGWVNMARLVRAQILKLKSQEFVLAARILGTNSRKIILKH
                     LIPNTMGVIVINTMFTIPSAIFTEAFLSFIGIGLSEPRASLGVLINNGYQVLHNFPHL
                     LIYPAIVIVLIMVCFSILGDGLRDALDPRMRK"
     gene            complement(13794..14732)
                     /locus_tag="PK898_04920"
     CDS             complement(13794..14732)
                     /locus_tag="PK898_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19411.1"
                     /translation="MGKYILKRILYLFITLLIIITATFFLMKRLPGTPFDSERFSQMP
                     PQQQKQLLEKYGLDQPVSVQYLKYLNNLIHGDLGVSFFYTGRTVKDVIFGRIWPSVLI
                     GLQAVLLGLAVGLTLGIIAAWKHNSGIDYFTMVLAVLGVSVPNFVVAALLQYYVGLKW
                     GILPVAFWGTWAHSVLPSIALSFGATAMFARFIRTEMLDVLEQDYIITARAKGLNQFQ
                     VLMAHAVRNSIIPVVTILGPIVVNLLTGSLAVESIFSVPGIGSLFVESIKSNDYATIM
                     GLTIFYSAFYILVVMIVDILYSVIDPRIRIQGNKDE"
     gene            complement(14953..16686)
                     /locus_tag="PK898_04925"
     CDS             complement(14953..16686)
                     /locus_tag="PK898_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU19412.1"
                     /translation="MKKLSLFILIISIVLIGLTGCSGSKESTTTAPATVSTESVSEPT
                     VEATAETEKETVATEETVATEPVTSGKPETASGTITVYDTNEIRTLVQWAASDTQSFT
                     VLNNVFEGLYRLGIDHQPEPALATGYTVSDDKLTYTFTLRDGIQWSNGTPITAKDFVF
                     AWLKQMGPDATNGYSFIMNDYIVNGTEYNEGKVKAEEVGVKAIDDKTLEVNLKTPTPY
                     FIRLTTLSMYFPLNEEFVTSQGDQYGVKAENMIYSGPYVITSYDPVVGTTLEKNEKYW
                     DAANVAVKNVNIKIIKDQSAALNAYKAGELSKVLLSSTDVAVYKNDPEFHSQSEFRTT
                     YLQFNTTAKGLDNVNIRKALGYSIDRSILADTILADGSAAAEGLVSFGVAGDGTSTFR
                     ELNGNLSPFDPEKAKEYWAKGVEELGEAPQLTLLIADDSVTKTVATFVQSQFKDNLGI
                     DVAIDSKTSKARNELMDNNNYQFAITAWGADYDDAMTYLDLWTNGTPYRGNYSNETYN
                     KLIADAKKETDEAKRLTMMLDAEKILLDTDAVISPLYYRGFAYLQKSNIENLVTHPFG
                     NPIEFKYARIK"
     gene            complement(16743..19337)
                     /locus_tag="PK898_04930"
     CDS             complement(16743..19337)
                     /locus_tag="PK898_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU19413.1"
                     /translation="MTLLFSEEKIQTIHRGYLQRLPSFGKSGVETARFIRELPEVERI
                     CMEFCYTFMPVQDFVSYPFQLIDIHVKHAVMLLNTVPWLKNIPEDIFLNFVLFYRVNN
                     EFIEDYKNRFFEELWPRVRDKSDRDAILEVNYWCYEKATYKESDDRTASPLTVIRRTY
                     GRCGEESTLLVSALRSVGLPARQCYTPRWAHCDDNHAWVEAYSDGKWHYLGACEPEPV
                     LDKGWFTAAASKAMLIHSKVFNSDMKGELIAGRTPVFCMINNLKTYAKTTCLNVFVRK
                     DNRPFAGAVVRCEIVNYSELYPLLTAETDDNGFASFVLGRGDIILHVHDGNHFLMKKV
                     DLRRQSEITVDFDEAVENKPGNFDFELIPPAEDLSNPVEISRETAEMHEKRLEHCNRV
                     RLQTEMSFAENPALKNLKLDSDWDKSLVKQYLTDAKGNFDEIIDFLQCDSFMTADKAA
                     ILNTLREKDFVDITSETLKEYLEASLPYKGLFPEDVYIHSVLAPRIENEMILPQRKQL
                     KAFFDRFSEPIRSASQLWDYLRKAVILQDEYGYDSLVCDAKNILSTGICDSHSLDILF
                     VSAARSLGIPARINSSTGRKEFYDGEKYCGIGEDSKPVDRNNSRLIIRNQSGKDLNYF
                     SQISIGKFSAGVYHSLVYWNQTIGEIWELPVEAGEYRVITTVRQIDGSVKCKVYFISV
                     PENQSVVLPVDLAKEDILGKLKYAKIENVPLMSESGENTDLYSELREDYTILAFADPG
                     KEPTEHLFNEWIRLAEKYRSPDLGVMIVLDDRRGLQNPTLQKVLQAIPGTRVLIMDNP
                     RYLKEFHLLMGVGDERKPFVLVINNHREGLYAFSNYNVGTGQTLLNIISEDSSGRKSE
                     KKPSGNIK"
     gene            complement(19399..20574)
                     /locus_tag="PK898_04935"
     CDS             complement(19399..20574)
                     /locus_tag="PK898_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU19414.1"
                     /translation="MMKTQKLDKDLMKKNNMKLVLACINEADEISKAQIAEKVQMSIM
                     SINRITDDLIQLGFVCETDRTKENSIGRPPMMLSLNRDNLLSLSVSLEKDTMRVGLVD
                     PFGEIKAIREYTITSSRFDPQEVLKQLAMNLQRFIDSQPDRKILPVVGIVLPGIVDNK
                     KGRMEFSSNLKWHNVQISKILKEYFPSKDFVVENDIKAIAIAENRFGASKQCHNSVVM
                     NIGNGIGAAVIIDNEIYRGKNNMAGEIGHIIINPVGQMCECGQIGCLQTKIAEWAILR
                     EAQSVEADITLEGLFARYDEKADWAVKLIDSVVDYISISINLLANTYSPEMILLCGSM
                     IQQNKKLRELIEKNYRRHLNDYIKNSFQLKFESFGRDGHIIGGSIIAYQRYIDEVIK"
     gene            20786..21529
                     /locus_tag="PK898_04940"
     CDS             20786..21529
                     /locus_tag="PK898_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOU19415.1"
                     /translation="MGNILVEVCCGSADDAVESFQAGADRVELNSDLFHGGLTPSIGE
                     LIIAKKHTGLKIMTMVRPREGGFCYTENEFATAVEDAKLLLAHGSDGLVFGFLKSDGT
                     IDVERTKIMKQIAGDKTTVFHRAIDVVPDWKSALDTLISLGITRVLTSGQESNVFFGI
                     ETVKEMITYADGRIEILPGAGITLKNIDRIVSETGCDQVHVAHYKPYSDSSVNNNREI
                     FYGGALYPPEDRFNMVDRDYISAIVNMTN"
     gene            complement(21668..22414)
                     /locus_tag="PK898_04945"
     CDS             complement(21668..22414)
                     /locus_tag="PK898_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU19416.1"
                     /translation="MKITVLGSNGWFDTDTGSTNCVLLQTSDYSVIMDAGFGITKIKN
                     RVDFSKPVYLFLTHLHLDHVIGLHALDYFNFEQPLRVITPIGGTDDLKDLLRSPFSAA
                     WDSHVYPIEMIDAKNLEKTNLPFKVEALPLVHAVPDTGYRFEIDGKIIAFVIDTAYCE
                     NAVKLAKNADLLITECGFYPGTVNTVSGHMTPETAAKLASDSNAKQTVLIHFGANAFE
                     TIDKRSRAVDTGRKVYPGLIMGLDNMEFIL"
     gene            22532..23338
                     /locus_tag="PK898_04950"
     CDS             22532..23338
                     /locus_tag="PK898_04950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOU19417.1"
                     /translation="MIPEIKDLCTFAEQAGVITIDGFGKTHQVTQKGIQDPVTEIDQK
                     SENFLIRQINHFYPGHTILSEESGLHNGTITAAEHVWYLDPLDGTMNYSHGVPFYSIS
                     IGYAFKGELVLGVIYDPMRHECYCAEKGKGAYLNGTKIQVSRETDLCYALLATGFNMK
                     IPETGRDNFPLFKHFMQTTAGVRRMGSAALEIAYTAAGRLDGMWEMHLSAWDVAAGFV
                     IAREAGAVVSNLKGDPNCFHVPFEFLIANPILHPLLLKEIELMIESKQTE"
     gene            23335..23724
                     /locus_tag="PK898_04955"
     CDS             23335..23724
                     /locus_tag="PK898_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU19418.1"
                     /translation="MKIDRTFLRNLILYGIIGAISAGFDALVFYLLFDKLAINKFVAN
                     VISVHFGIFLSFFLNSFYNFKKTDKMGKRFFSFYLTGLFGLGLSTLILYAGGRLRIDV
                     MATKVFSVVFVALVQFIINRAVAFGDK"
     gene            23759..26002
                     /locus_tag="PK898_04960"
     CDS             23759..26002
                     /locus_tag="PK898_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOU19419.1"
                     /translation="MNRTEILLGILAGLFISQKIRVPLPIWLIFLLLFAVLAVFCGKK
                     SSKYVLDQPVKKAAMIALTAAFCCLGGLRGSVTNQPLPSGHLSEVSGEEVSFKGIINR
                     PVTRSDSQTRLRIRLVRNEDSDSDPDRGECIVVLNSLTNSFEYGDLLSVRGKLTKLDA
                     SAPNSYGSYLERSGISGILYNPEIELIAKQQGNPILNRIFRLQSYLLQKTYDIFPEPE
                     NSLMAGILLGDKSRIPAEIEEAFRNTGTAHIIAISGMNFSVLIWMLLSVLRAFHKRWW
                     TPLILLPFIFFYTILTGANPAIVRAGIMGGIALIGMSIGREKGGVDSLLLSVAAMAMF
                     QPKILFDVGFQLSATATLGILIFNPPLNSLIEKGFTAIHLKNNVQKILLDTLNEILIT
                     SFSAQIFTTWIIAASFHQFAIFSLIVNMLIAPFQNLIMVGGVFALFLSILSESLGKIA
                     GLIVDPLPALTIRIICFFGKFDWASQYSDLSNLAAWIIIFSILTVWINRNRWIRQKKE
                     TLIQAGITILALFTLVCWRQVLAKADHRLHLTFTGGTNTSSILIRLPESQKILVANGV
                     TNFQAQNLLKKNPFDPADYAAAILDFQEEWMADEYLRSDDEKPEWLILNGRNRSLSAE
                     QTNPTPVYLNEGFQLRTNPFILTLQQEYLKRKRWIISYRSLTIDIPNGIPLHRIDTLN
                     DPNPSVSIILLGKKDNEREWEEYLSQTYCHGSSCSGKPILLSTNEYGTIEVISDGEKI
                     WLVQQSS"
     gene            26179..27456
                     /locus_tag="PK898_04965"
     CDS             26179..27456
                     /locus_tag="PK898_04965"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOU19420.1"
                     /translation="MLPLDNVLAVILGGGRGTRLFPLTQLRSKPAVPIAGQYRLIDIP
                     ISNCINSGIFKIAVLTQFNSVSLHRHILDTYQFDRFHSGWVQIWAAEQRTDNNDWYQG
                     TADAVRKQLSEIISSRTEYVLILSGDHLYRCNYADMLQFHLDHHADVTIATQPVHKDE
                     ADRFGILKRDSEFKINDFKEKPKDPEVLKELVSLDDEERPYLGSMGIYLFNKDLLVGL
                     LNALDYSDFGKHIIPYAIEKYDVYGWTFDDYWEDIGTIRSFYETNLMLTEDHPKFNFY
                     DPKHPIYTLNKTMPTTKIENTEVKNALIANGCMIKANSIEHSIIGIRSMIDVGTTIKD
                     SILMGVDDDVFEKSANDSKYFDIPLGIGKNCHIEQAIIDKNARIGDNVIIKPFPPHIE
                     LDDADPIKKYYVRDGIVIIPKNTSIKSGTVITP"
     gene            complement(27518..28156)
                     /locus_tag="PK898_04970"
     CDS             complement(27518..28156)
                     /locus_tag="PK898_04970"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU19421.1"
                     /translation="MKKNPTLDYEKALWEQGFFYVAGIDEAGRGAWAGPVMAGAVIFP
                     VDENLPVILDGVRDSKQMTPETREEKFRIITDYAIGWAVGSASNWEIDSMGILPATRL
                     AMDRAIAQLQPLPQYLLIDYVRLFRQMMPQKSIIKGDECSYSIAAASILAKVCRDHWM
                     VEHCAEEFPQYGFEHHKGYGTQFHQEALEKFGPCKYHRKSFHPIASELKLFE"
     gene            complement(28153..28905)
                     /locus_tag="PK898_04975"
     CDS             complement(28153..28905)
                     /locus_tag="PK898_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU19422.1"
                     /translation="MESTINPSSHIYLSIILPAHNEEKRLPICLEKVIAFVSSLRFPF
                     EVIVVENGSVDQTLAIATEYAKRYDWLRVLQNSKPGKGLAVRTGMLAAKGEYRFFADV
                     DFSMPIEEILNFLPPKLSDYDVAIGSREVKGAIRYNEPPIRHLTGRVFNQIVRLMTVH
                     NIQDTQCGFKCFTANAAEKIFSVQVLDGWAFDAEVLYIAQKNGFKIVEVPVRWYYDGQ
                     SKVHVLNDSVKMFRELLQIRCNDKSGIYKIEQ"
     gene            complement(28991..29063)
                     /locus_tag="PK898_04980"
     tRNA            complement(28991..29063)
                     /locus_tag="PK898_04980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29028..29030),aa:Pro,seq:tgg)
BASE COUNT         8192 a         6597 c         6261 g         8131 t
ORIGIN      
        1 ttgtcccaat taacataatt gatcatcctt atcccatcga tatggtaaaa tcgaaatata
       61 aaaagtgaga ttatgggagg aaagaatgct caagaaagaa ttattagaca ttctgcgctg
      121 tcctgtctgt gtaaaagaga ataaagggaa tctgatcaat tacaaagatt cctggttaat
      181 ctgcgaagac tgtcagcgaa aatatcccgt tgtggaagac attccggtaa tgttgatcga
      241 agaaggctcc aaatggatcc aaaccgaccc ggaaaatctt caaatcccac cggaaattta
      301 agaaaaagaa gttgagtttt atggcacaaa caaattatcc acagaatcag gattcgaata
      361 aagaatatcc gaataatcga gcgccgcaaa attacggcat tccggggcaa tttccgccca
      421 attttaatcc gcaacaacct catggggcaa tacctcccca accgcaaccc aagaagaaga
      481 aaaacaataa aaaatgggtc attttgggcg tttttgcact cgtcggtttg gctatcggga
      541 tttttctctt caccaaggtg aatcaatacg taaaagcgat gaccgtcatc gacttaccgg
      601 gcgcacccgt aatcagtgat aacgggaagg ttgctgttga aaccgaaagc aatccggatg
      661 ccaccgaaga ggctgtctca ctgagtgata ttacaaaggt gaaaggtcag ccctgggatg
      721 gggtttcaag aattacctgc cttgcaatgg gtttggatta tcgggattgg gaagcacagg
      781 aaaaatactc acgtacggat tccatgatgt tactgaccta tgaccccgtc acaaaaatgg
      841 ccggtatgct ttcgttgccg cgtgacttat gggtggcaat tcccaatcac ggttacggac
      901 gtattaatac cgcttactat ttaggtgaag ccgaaaacct gccgggagga ggtcctcaac
      961 tcgcaatcga taccgtcgaa ttattcctcg gaatcgatat tcagtattac gctgtgattt
     1021 cttttgaggg tttcgtcaat ttcattgatt ccatcgataa attggcaatc aacgtcaagg
     1081 atcagatcac ggttgacccg atcggtccgg gaaacaccgt cacactctac cccggtgtgc
     1141 aagacttaga cggtgcgact gcattggcat acgcccgtca acgatatacc gaaggcggcg
     1201 attttgagcg agcccaacgc cagcaggacg ttatttatgc tttgtacgaa caaatggtct
     1261 ggcagctgcc tcaacttcta ctccacgccg ataagcttta caacaccata tcccaagcga
     1321 tcagaaccaa tctgactttc gacgatatta tcaaactggc atggacggtg gtggatatca
     1381 aaccggaaca aatcgtccga agcgtcatcg ctcctccgta tcaggtacag tacggtaaaa
     1441 cctatgacgg tactcaggat attctcattc caattccgga taaaatccga gaagcaaggg
     1501 atgtcatttt cagcaccagt cttgccgcat ctccggctac cgttgccgat gatcaggcga
     1561 ctttgatcgc tgcggaaggc gctcatatca gcctattgaa cggctcgaca aatcccgata
     1621 ttacggacaa gaccgttgcc tatctgcaac aattcgggat cacaatcgat tcaatcggac
     1681 aagcggcgaa ttcttataca aattcgctgg taatcaataa agggaaaccg tttacggcga
     1741 aattcctggc agatctgatg gccatcccaa ctgctgcgat cacaatggat tatcaaccgg
     1801 acgcagcctc ggatttggtt ctgaccatta cggacgcttg ggcaaacagc aaccccatgt
     1861 aacaataacg caactccgga tttcatgtaa catccggagt tgtaatttat caacaatcgg
     1921 agcaatatgg aaaaaccagt tgttttagtc attatggatg gcgtcggtat cggaaagggt
     1981 gatataggtg acgccgtaaa aaaagcgaat acccccacat tggattggct gcgcagccat
     2041 tgtccgtata ctcaaatcaa agctcacggc acagcggtgg gacttccatc ggatgaagat
     2101 atggggaaca gcgaagtggg tcataacgca ctcggctgcg gtcaaattta ctcacaggga
     2161 gccaaactcg tcaatgagag cattgagaac ggatccattt ttcattccga aacctggaat
     2221 aaactgatcg gaaattgtgt agaaaatcat tcgacactcc attttatcgg gcttctttcc
     2281 gacggaaatg ttcattccaa tatcaatcat ctgaaagcga tgattcggga agcaaaaaat
     2341 caaaaagtct ctcgcgtgcg gattcatatt ttattggacg gccgagatgt cccctcgact
     2401 tcggcactgc aatatgtcaa tgatttggaa tcttttttaa aaacgctcaa cgacaattcg
     2461 tttgacgcca ggatcgcatc cggcggcgga cgaatgaaag taacgatgga ccgctaccag
     2521 gcggactgga atatggtcaa gatcggttgg gatacgcacg tactgggcat cggcaggcag
     2581 tttgccaacg ccgcggaagc gattgagacc tatcggaaag aattagaggt aatcgatcag
     2641 gatctgccgg cttttgttat tgccgagaat ggcaagccga tcggaaccat tgaagataac
     2701 gacagtgtta ttttcttcaa tttccgtgga gatcgtgcga ttgaaatcag tatggctttt
     2761 gattacccgg actttaataa atttgaccgt gtccgttatc cgaaagtttt ttatgcgggt
     2821 atgctgcaat atgacggcga cctgaagctc ccaaagaatt tcttggtgaa cccgccgaac
     2881 attcaacata cgcttacgga attgctcgtc agccggcacc tcagccaata tgccatttcc
     2941 gaaactcaga aatttgggca tgtcacctat ttctggaacg ggaaccggag cggtaaatta
     3001 agtgaagaat tagaaacttt cgaagaaatt ccttccgata tggtgtcttt tgacgaacga
     3061 ccttggatga aatcggctga aataaccgat aaaatgctga gcgcgatctc ctccggaaaa
     3121 tatgatttta ttcgctgcaa ttatccgaac ggagatatgg tcggacacac cgggaacttt
     3181 gatgcaacgg ttatcgccgt cgaagcggtt gatttggaac ttgcgcggat cgttaaactg
     3241 atggaatcac atgattttac cttggttgtt acggctgatc acgggaatgc ggatgatatg
     3301 atcgagaaga caaagtccgg agaattacag ccgaaaactt cccattcgct caatccggta
     3361 ccttttatga tttacgataa aacccgtgag tggaaaatca aagagggcag ctacggtttg
     3421 gcaaacgtta ccggaacact ggtgaaatta atgggattgg aaccgccgga aatttgggaa
     3481 aaaccgatga tctgatcggt gacggaacgc gcgaaacaaa aaaagaatat ggttcaaaaa
     3541 aggatcggat ttgaaaatcc gatccttttt tatgaaagac cgattttctt cgttatcagg
     3601 taactttttt tgattcctct tttatgaatt tttgcatttt tccaaataat ctgaatattt
     3661 gttattaaca ctatgttata aacatcaact tattccgggt tagaattact tattaacgtt
     3721 ttaatttaac gcttatttca gttcgatttg gacaaatatc acatttaatt aagattaatt
     3781 acttgagtgg ccatgttatt attgcatata atgttaataa taaagcagaa aatattcgtc
     3841 cgactttgtc gataagcgag atttgaatcg ttcgattcca tagtgtatat gcaggagaga
     3901 tttcctttcc tgcgaaaatt ttgaaaggag atttatatgt acaaaagaaa aatcttattc
     3961 gtttttattt ttctgaccgt ttttgctttt gccgctcagg gattgttgac agtatcggca
     4021 caatccgaca atgccgcgaa agccccggat caggatttgg ttattgcttt caataacgat
     4081 atccgggcgc tcgatccgca taatgtctca gacacgctga ccatctctgc gacacggacc
     4141 atgtatgaat cactcgtaaa attcaacgag aaacaggaaa tcgtacctct cctggctgaa
     4201 agctatgaaa tttcggatga cgggttgact tatacgttcc accttcgctc aggcgtgctt
     4261 ttccatgatg gcaccgaatt caattcggat gcggtgaaag ccaacatcga tcgctgtaag
     4321 gatgaaagtt tgcgacagaa tcggattgtg aagcagattg cgaatgccga atatcccgat
     4381 ccgtataccg ttatcctgac acttgcaaaa ccgaacaaca ccttcctgaa taaaatgacc
     4441 atgttcgatt tcatcgcacc cgcgacgatt gccgcaggcg aagagaaaat cctgcgggaa
     4501 ccctgcggga caggccctta cacattcaaa gatcgtgtgg aaggcgatca tgttactgtc
     4561 gttccttttg aaaaatattg ggacggagcc ccacaggttg catcgttgac cttcaaagca
     4621 gtcccggaag acggcgcccg agtcgcaatg ctgcaaaccg gtgaagcgga ttacatttat
     4681 cccatgccga ccactcagat taaggtcgtc gaagggaaaa aagatattct gatcgatgca
     4741 aagacctcca atatcatgcg gtatgttacg ctcaataccg agttggaaca attaaaagac
     4801 aagcgcgttc ggcaggctat caattatgcc gttgacaaag acgcatatat caaaaccgtt
     4861 ttcaacggtt atgcttccaa agtctattcc tgctatcccg agacgatcca atactatgct
     4921 cctcagcctc cttatgactt taatcttgaa aaagccaagg cactgatgaa agaagccgga
     4981 gcggaaaacg gatttaaact tacgatttgg ggagataaca ttacctccga agaaaaaggg
     5041 atgcaattca ttcaacaaca attgaaacaa atcggaatcg atgtcgaagt aatgccgatg
     5101 gaagccaatg tcgtcgcaga aaaaatccaa gccgaaagag ataaagccga agtgaatatg
     5161 tggtatgtaa attggtcggc ttcttcattc gatgcggacg gtgcgattcg cgcaattctg
     5221 cacggagaga gcatcccgcc gggtcaatat aactcagcat actataacaa tgaagaagtc
     5281 aacaaactga tggatgaagc cttggcaata accgatccga agattcttca ggaaaagtac
     5341 gcaagaattc aggaaatcgt ctgggatgac gcgccgtgga tcttcttggc gagtgatcag
     5401 gtgatcagcg gttacaaatc ctattgcagc ggcatccagc tgtatccgga cggagccatc
     5461 gattgttcaa aagcgaaatt gaattaatcg agaatccatt cgaccggtca tctcggttgg
     5521 ttgaaatcag gtaaccggca aaatgatctt caaaaaccct gcatatcagt tgcggatcga
     5581 tcacaatcta aatccgaact ccggattttc acgagcgatc caaatgaatg cagggttttt
     5641 cttcaaccgt aagttattat taaaaagtga aagtttcgtt gatctgaaaa gttttaaccg
     5701 gcgaaacata tcgatcaaat cggaggtatt ctttgggccg ctattttgtt aaaagagtat
     5761 tgggaaccat tccgttgctt ttggtaattt cttttttgat ctttatgttt attcatttga
     5821 tccccggtga tccggtacga aatatggcag gtcgagaagc gacaaccgaa gaactcgaaa
     5881 acctgcggga aagcatgggg tttaacaagc cgctttatca gcaatacttt tcgtatttga
     5941 atgatctttt tcatggagat ttgggaattt cgtataagac gaaattgccc gtttcggaga
     6001 tgatgatcgg ccggttaaag cccacattaa aactgacttt ctttgccatg ctctggtcaa
     6061 ctatcgtggg aattgccatc ggcgtccttt ccgccataaa tagaggaaaa ctgatcgatt
     6121 atttgggaat gctcatcgcg atttccggaa tttccatccc ggggttttgg ctcggtctcg
     6181 tcatgatcca aatcttttcc gtcaaactgg gctgggtgcc gaccggaggg ttggatacct
     6241 ggaaaagtta cttcctgcca tccatcacgc tgggatgcgg tattatggcg attctcgcac
     6301 gtttttcgag atcatcgatg ctggattcac tcggggaaga ttatgtgcgg accgctcggg
     6361 caaaaggcca acgtgaaaga ttggtcattc tgcgccatgc tttccggaat tctttggtac
     6421 aggttgtaac ggttatcggc cttcaaatcg gcgggctctt ggccggatcg gtattaatcg
     6481 aaaccgtgtt tacgattccc ggattgggaa ggcttctggt cgattctctc gctttccgcg
     6541 attataaagt cgttcaggcg ttattgctgt ttttctcttt tgaatatatc ctgattaatc
     6601 tgattgtcga cctgctttat ggtgtcctca atccgagaat tcgattaaag taacaggaaa
     6661 agaccatgca gacatcaacc ttcatcgatc aacctataaa aaaagccgaa aacagaacaa
     6721 aatactattt tcgtaaattt ttccagcgga aaacagcggt catcgcggcg gttatcatcc
     6781 tgatcttcct gttctttgct atttttggtc ctcagatagc tccttttgat ccttctttac
     6841 cggattacga taacctatac ggagctcctg atgctgtcca ttggtttggg acagacgaat
     6901 tcggacgcga tgttctcagc cgtttgttgg tcggaacgcg aaattcattg ggtgtttcct
     6961 tggcttccgt tctgatcgga gctgccatcg gcacattttt gggagtcctt gccggatatt
     7021 acggcggaat catcgattcc gttgtgatgc ggggctcgga catcctgttt gccatgccgg
     7081 atattttgct ggcgatcgca attgttgcaa taatcggtcc cggaatcatc aatgttgtca
     7141 tcgccgtcgc ggtgttcacc attccttctt ttgctcggat catgcggagt gccaccttgt
     7201 ccgtcaaaag ctctctgtat gtggaagctg ctcgttccat cggctgtaag aacagtcgta
     7261 ttttatgggt acatattttt ccgtcaacga ttcaatctat gatcgtcaat tttacgatgc
     7321 gtatcggcac tgcgatcctt gcggcagcct ccctcagttt cctcggttac ggggcaaaag
     7381 tcgacgaacc ggattgggga gcaatgcttt cttccggacg aaattatttg gatatcgctc
     7441 cgcatttggt ctatttcccc gggctgctga tttttatagc ggttctggca tttaatcttc
     7501 tcggggacgg actgcgcgat acgttggatc cgaagttgaa ataggacaca gaaaaatgag
     7561 cgaaaataac cttctgaaag tcgagaatct caggaccgaa tttaaaatca gccggaaaaa
     7621 gtccgttgct gccgtcagtg atatcacctt tgaaataaac aaaggcgaga ttgtcggttt
     7681 ggtcggagaa tcgggctgcg gaaaatcagt cacatcgctt tcgattatcc gtttgttaaa
     7741 tgatacttct ggcgaaatta cccatggatc cgtctggttc aagggaagca atttactgaa
     7801 attatccgac gaggaaatgc gccaaatccg cgggaaagaa atcgcgatga tttttcagga
     7861 accgatgact tcattgaatc ccgtcctccg gattgataat caactcatcg aagcgattca
     7921 acttcatacc gaatacacaa aacaggaagc tcgtgagcac gcgttggaaa cgttaagaag
     7981 tgtcggaatt cccgatccgg agcgtgtcag ccgctgctat ccccaccaat tatccggagg
     8041 aatgagccag cgggtaatga ttgcgatggc aatgtcctgc tacccggatt tgttgatcgc
     8101 agacgaaccg accaccgcat tggatgtcac gatccaagct cagatcctcg atctgatgca
     8161 gggaattcga aatacccgcg gcacaagcat cttattgatc acacatgatt tgggtatcgt
     8221 tgccgagacc tgttcccgtg tttatgtgat gtatgccgga agaattatcg aggaagcacc
     8281 ggtagacatc cttttttccg atccgagcca tccctataca caaggtctca tcgcgtccat
     8341 tccgaaattg gggttggaag cggaaagcct gccgtttatt ccggggagtg taccggatat
     8401 caccatgatg ccgggcggat gtcggtttca tccccgctgc cgctatgcca tggaaatctg
     8461 cagccaaaaa gaacccgaat tgatctcaat caatgaatca caaaaatgtc gatgctggtt
     8521 gaaaacatcc gaatattcca taaaaaccga gaacggagaa agcagaaaat gactgatttg
     8581 ctgaccatca acaatctggt aaaacatttt ccggcggaga aaaaactttt cggaaagcag
     8641 gattacgttc atgcggtgaa taatgtttct ctggaggtcc gggaaggcga aacgttctcc
     8701 gtcgtcggtg aatcgggttg cgggaaatcg actgtcagtc gtttgatcaa ccatctgatt
     8761 caacccgatt ccggtgaaat ttggtttgac ggaacggaaa cagcacatct caatgaaaat
     8821 gagtttcggc cctttcgcca acatatgcag atgatcttcc aagatccgaa ttcttctctg
     8881 gatcccaaca tgcggatctt tgatttaata tccgaaccgt tgttgatttt taccgaaatg
     8941 tcagatcggg aaagacggga acgcgtccgc gaattattgg aagtggtcgg cttaagttac
     9001 aatcatgccg aaagatatcc gcatgaattt tccggcggac aaagacaaag gatcggcatc
     9061 gctcgtgcct tatcggttaa ccccaagctg atcattgccg acgaaccgat ttcgtcgtta
     9121 gacgtatcca ttcaagctca aatattaaac ctgttacaga aattacaaaa acagttcaac
     9181 ttaacctatt tttttatttc ccatgacttg agcgtcgttc aattaatctc ggatcatgtt
     9241 gcggtaatgt atttggggtt catcgttgaa aaagcaccta aaaaagaact ttatcaacac
     9301 ccgcttcacc cttatacaca agcattacta tccgctgtcc cgatccccga tcccaaaatt
     9361 aagaaacaac gtattttatt ggcgggggat ctccccagca atattcactt gcctgcggga
     9421 tgccctttca attcccgttg ttttcgctgt caggatcgat gccgagaaga gaagccggaa
     9481 ttacgtgaaa tcgaaaaaga tcattttgtg gcttgtcatt ttgccgatct gtgaccattt
     9541 aagcaaataa tcctgtcccg ggatgaatgc ttttcgaaat ttgattagcc gtcaagcagt
     9601 tcaaaccgca catctgcctc ttgcgtctca acacaaatga cgacgaccat ttggttttcc
     9661 gcatctggcg gaaggaaaat ttcccagtag ctgtcattct ccaacgtttt tcgataattg
     9721 aacgctatcg gtcgatttcc gcgcaaaatc ttggcgcttt gaggaatatt ggcaatattc
     9781 ggtaaggcaa cggttaattc cggaaattgt cccggatgaa agacagttaa aaacagtcga
     9841 tgaggtttat gagtgaaata gatatccggg gtttcgtaaa cataaggagg gaccgcctcc
     9901 gccccataaa atgcttcacc gttttcggac aagaattcac cggcttcatc cattattttg
     9961 gtgctctctt gcggaatttt gccgctgccg tcggggccga cattcagcag ataattgccg
    10021 ccgcgactga caatcttcac cagtttgcga atcactgttt ccgcggaatc ccagtttgta
    10081 tccgatttct tatatccgaa tgaacgattc aacgtcccgg gaatttccca ttttcgcttc
    10141 aaagggaaag cgggcaagcg attatcttga gtacagaaat aatcacccaa tccgttcccg
    10201 atcctcccgc tgaccaaaca atcgggttgt agttttttaa ccagttgata aatctcttcg
    10261 cactcttccg ctttcatccc catcggtgta tcaaaccaca tcattccgat cggaccgtaa
    10321 tcggacagca actcggttaa ttgcggaagg cacttttcat caagatatcg gcgaaaattt
    10381 ttattttgat tgtcatgata agcgacataa ccatccggat gatgccaatc ctgagcttgt
    10441 gaataatata aacaaaagac gattcctttt ttccggcaag cctctgccaa ttcggagaca
    10501 tagtcccgtc gggatggtgt ccggcagaca ctgttatagt ccgaacagga tgaaccaaac
    10561 aaagcaaacc cgtcatgatg tttcgcggta aaacagagat aacgggttcc ccaacgaagg
    10621 gcggattccg cgattttgtc ggcatcaaat tcaaacggat caaattgagc ggcaagctgc
    10681 tcatattcag acagcggaat attcaagtca ttcatgatcc attcggaaaa gaacggcgta
    10741 acttttcctt tgtattcgcc gcccaaaagg gaatacaagc caaaatggat aaataatcca
    10801 tatttcgcat caagaaacca gtttttcgga tatgaactca taaaggaatc ctttcgatcg
    10861 gatttttcgc agttaaaaat cggttttcag cgatccgtaa gatgacaggc gacaaaatgc
    10921 cccggtgaca cctcaattaa ttccggagaa acctctttgc atatttccgt cacctgcgga
    10981 catcgggaag caaatcggca tccgcttccc aaattaatgg ggctgggcag acttccctgc
    11041 agcacaatcc gtttccggct tttttccaaa ttcggatcag ccaaaggaat cgcagacaac
    11101 aacgcttgtg tatacggatg caaagaattt gcaaataatt catcagcggt tgttaattcc
    11161 accagactgc ccagatacat cacaccgacc cgatcgctga tatgtttaat catagataaa
    11221 tcgtgggcga taaacaagta agtcaatccg cgcttttcct gtaaatcctg aaggaggttg
    11281 acaatttgag cttggatcga gacatccaat gcggaaatcg gttcatcggc aatgacaaaa
    11341 tccgggttga ctgcaagcgc tcgggcgatg ccgatccgtt gtcgctgtcc accggaaaat
    11401 tcatgcggat aacggtttgc atgttccctt tgcagtccga ccgtttgaag cagctccact
    11461 accatttcct ctcgctcttt cttatcggca gctagatgat gagcatcaat cccctcagca
    11521 ataatatcca tcactttcat gcgttgattc aaagaggcat acggatcttg aaagatcatt
    11581 tgcgcccggc gtgtaaattg aaaactttcc cgggaagtta aatctttggt caaattcata
    11641 ccgtcaaacc aaatttctcc gtcagtcgca tgataaagtc gaatcaatgt tcgtccgaga
    11701 gtcgattttc cgcagccgct ttctccgacc aacccgagtg tttcacccgg ataaatatcg
    11761 aacgtcacat cgtcaaccgc cttcaaggtt aggttctttc ctacattaaa atacttcttt
    11821 aaatttttaa ccgaaaccaa aggctgttta tccggcattt cgcacctcgt taatcattct
    11881 ttttgcccgc ggatcgtgca gccagcaagc ggattcgtga tggttctcaa aaggagtaaa
    11941 ggccggtgga tattgccggc aaacctttaa agtatacgaa catcgagcgg caaaaggaca
    12001 gcctttcgga ggatctgtca gatcaggcgg ggttcccaaa atcgtaaata acttgtccgt
    12061 tttttcatga agtttcggta cggcagccaa caatcctttg gtatacggat gttgcggatg
    12121 atagaaaata tcttccacgc tgccgttttc cacaatcttg cctccgtaca tcacataaac
    12181 gcgatcggcg atattggcaa ccacgcccaa attatgagta ataataatga tagaagtatt
    12241 aatcttttct tgcagttctt tcattaaatc aaggatttgt gcctggatgg ttacatccaa
    12301 agcggtcgtc ggctcatcgg caattaatac cttgggatca caagccaaag caattgcgat
    12361 aatgatccgt tgccgcatac ctccggataa ttgatgggga tattgcttga aacgggtctc
    12421 gggattggaa attcccacca aatcaatgat ctcaatagcg cgtcgtttca tctcatcccg
    12481 acttaaatct tttcgatggt ttttcaatac ttcgacgatt tggttcccga ccgtcatggt
    12541 cggattcaaa ctggtcatcg gatcctgaaa gatcatggat atatccgagc cgcgtatttt
    12601 cagcatctct ttttcgctga gcgttgtaag ctctttcccc tccaatcgaa ccgatccttc
    12661 ggtaatcgaa ccggttgaag gcagcagttg cataacgctt tttaccgtaa tcgtcttacc
    12721 gctgccggat tcaccgacga tggcaacggt ttccttttta tccaggtcaa aactgacacc
    12781 gcgaaccgct tgaattctgc ccaattccgt tttgatttcg acatggagat tctccacctg
    12841 taatattttt cccattcagc ctcctatttc cgcatccgcg gatcaagcgc atcgcggagg
    12901 ccgtctccca aaatactgaa acagaccata atcaaaacga tgacgatcgc cggatagatc
    12961 aaaagatggg gaaaattatg aaggacctga taaccgttat taatcagaac ccccaaagat
    13021 gcgcgaggct cactgagccc gatcccgata aagctgagaa atgcttcggt aaaaattgcg
    13081 ctcgggatcg taaacatcgt gttgatgaca atcacaccca tcgtattcgg aatcagatgt
    13141 ttcagaatga tttttcgtga atttgtaccc aaaattctcg ctgccaaaac gaattcctga
    13201 ctttttaatt taaggatctg agcccgtacc aatcgagcca tgttgaccca gccggtgatg
    13261 gacattgcga taataatggt tccgataccg gccggcatca ccatcattaa cagaatgata
    13321 atgatcagac tggggattcc caccaaaatt tctatgatcc gctgcatcac cgcatccact
    13381 ttacctcccg ccaatgcgga aaccgctccg taggtcactc cgacaaatat atccaaacag
    13441 gcggcggcaa aggcgattaa taatgagatt cttgtaccgt accagatccg ggcaaattga
    13501 tcacgtccga attgatccgt gccgaaaatg tgctcccttg atggaagctg aaatgtgtga
    13561 tgataatccg ttttagcgta atcgtagctg ctgaaaatcg gtccgaaaac cgcaaaaagg
    13621 aaaataagaa tcaaaataaa aaggctcaca acggcggctt tattcttttt taaacgaatc
    13681 cagccatcct gaaaagccgt caaactgggc tttgcgatat gttctttttc atccagattg
    13741 acttccgctt tttcgaataa ctcaagtggt aaatcccgat aattttccat ggcttattcg
    13801 tccttatttc cctgaatacg tattctggga tcgatgacgg agtagagaat atccacgatc
    13861 ataaccacca aaatataaaa tgcactgtaa aaaatcgtta atcccataat tgttgcataa
    13921 tcattgcttt taatactttc gacgaacaga ctgccgatgc cggggaccga aaaaatgctc
    13981 tccaccgcca aggatccggt caaaagattg accacgatcg ggcccagaat cgttactacc
    14041 ggaataatcg aatttcggac cgcatgggcc atcaacacct gaaattgatt caatccctta
    14101 gcgcgggcgg tgataatgta atcctgttct aaaacatcca acatctcggt tcggataaaa
    14161 cgggcgaaca tagccgttgc gccaaaggag agcgcaatcg atggcaaaac ggaatgtgcc
    14221 catgtccccc aaaaggcaac cggtaagata ccccatttca aaccgacata atattgaagc
    14281 aacgcggcaa caacaaaatt cggaaccgaa acacccagaa cagccagaac catagtgaaa
    14341 taatcgatgc cgctgttatg tttccaggca gcgataatcc ccagcgttaa tccgacggca
    14401 agcccaagta aaacagcttg taatccgatt aaaaccgaag gccaaatcct gccgaagatc
    14461 acgtctttaa cggttcttcc ggtatagaag aacgaaactc ccaaatcgcc gtgaatcaga
    14521 ttattcagat actttaaata ttggacagaa acgggttgat ccaatccgta tttttcaagc
    14581 aactgttttt gttgttgtgg cggcatttgt gaaaatcgtt ccgaatcaaa cggcgttccg
    14641 ggcaagcgtt tcatcagaaa gaaagtcgcg gtaataataa tgagcaatgt aatgaacaga
    14701 taaagaattc tttttaaaat atacttcccc atatcactcc tttcttcctt gtttgaacaa
    14761 agggctcttc atggttttga tggtttcgtg attctttgag ataaatatca agatcattgt
    14821 ttttcttcga aatttgaacc gccagaaatt tactttactg cttttgtcaa aaaccgatcc
    14881 ccgacagccg gtaaaaattc tcaccggctg ccggttatct ctatttttct tttcgatcaa
    14941 aaaacgaaaa gactatttaa ttctggcgta tttaaactca atcggatttc caaacgggtg
    15001 ggttaccaaa ttctcgatat tactcttctg caggtaagca aatccgcgat aataaagagg
    15061 cgaaattacg gcatcggtgt ccaataaaat cttttcggca tcgagcatca tggtcagccg
    15121 tttcgcttca tctgtttctt ttttcgcatc cgcaattagc ttgttatagg tttcgtttga
    15181 gtaattgccg cgataaggag ttccgtttgt ccataagtcc agataggtca ttgcgtcatc
    15241 ataatcggcg ccccaagcgg taatagcaaa ttgatagtta ttgttatcca tcagttcatt
    15301 gcgggccttg ctggtttttg aatcgattgc gacatcaatc cccaaattat ccttaaactg
    15361 gctttgaacg aacgtggcaa ccgtctttgt caccgaatca tcggcaatga gtaaagtgag
    15421 ctgcggcgct tcgcctaatt cttcaacacc cttcgcccaa tattctttcg ctttttcggg
    15481 atcgaacgga gagaggtttc cgtttaattc gcggaaagtc gatgttccgt caccggcaac
    15541 accgaaggaa acgagtcctt ctgcagcagc ggaaccgtcg gcaagaatgg tatcggcaag
    15601 gatcgaacga tcgatcgaat aacctaaagc cttacgaatg ttgacattgt ccagtccctt
    15661 ggcagtcgtg ttgaattgca ggtaggtagt ccggaactcg ctttgggaat gaaattccgg
    15721 atcattttta taaaccgcca catctgtcga ggacaataag actttggata attctccggc
    15781 tttatatgca tttaatgcgg ccgattggtc tttgataatt ttgatattca cattcttgac
    15841 cgccacattt gccgcatccc aatatttttc attcttttcc agagtcgtac cgacgacggg
    15901 atcatagctg gtgatcacat agggaccgga ataaatcata ttctcggctt ttacaccgta
    15961 ttgatctccc tgggaagtga cgaattcctc attcaacggg aaatacatgc tcaatgtcgt
    16021 caaccgaata aaatagggag tcggggtttt taagttaact tccaacgtct tatcatcgat
    16081 tgccttgacg ccaacctctt ccgctttgac tttgccctcg ttgtattcgg ttccgttgac
    16141 aatatagtca ttcatgataa acgaataacc gtttgtggca tccggaccca tttgctttaa
    16201 ccaagcaaaa acaaaatctt tggcagtaat gggagttccg tttgaccatt gaatcccgtc
    16261 gcgtaaggta aaagtgtaag tcaacttgtc atcggaaacg gtataaccgg tcgccaaagc
    16321 cggttcgggt tgatgatcga ttccgaggcg atacaaacct tcaaagacat tgtttaaaac
    16381 ggtaaaggat tgagtatcgg aagcagccca ttggaccagt gttcgaattt cattggtgtc
    16441 ataaacggta attgttccgg acgccgtttc cggtttaccg gaagttaccg gttccgtcgc
    16501 cacagtttct tccgtcgcaa cggtttcttt ttcagtttcg gctgtcgcct ctacggtcgg
    16561 ttccgaaacc gattcggttg aaacggttgc cggtgcggtt gttgtacttt cttttgaacc
    16621 tgagcagccc gtcaatccga tcagaacgat gctgatgatt aaaataaata acgatagttt
    16681 cttcattttt tctccttaat atatttaaaa tgcggatggc attttatttt ccgtttgaaa
    16741 aatcatttta tatttcccga tggtttcttc tctgatttcc gccccgacga atcttcgctg
    16801 atgatattca gcagggtttg tcctgttccg acgttatagt tactgaaggc atataatccc
    16861 tccctatgat tattgatcac caaaacgaac ggttttcttt catctcccac tcccatcaac
    16921 agatggaact ctttcagata acgggggtta tccataatca aaactctggt tccggggatg
    16981 gcttgcaaca ctttttgcag tgtcggattt tggagtcccc ggcgatcatc caaaacaatc
    17041 atgaccccta aatcgggcga acgatacttt tccgccaaac ggatccactc attaaacaga
    17101 tgttcggtgg gttctttgcc ggggtcggca aaggcaagaa tggtataatc ctctcgcaat
    17161 tcggaataca gatcggtatt ctcacctgat tcactcatta atgggacatt ttcgattttg
    17221 gcatatttca gcttgccgag aatgtcctct ttcgccaaat caaccggtag aacaacactc
    17281 tgattttccg gtaccgatat aaaatacacc ttgcatttca cgctgccgtc gatttggcgt
    17341 acagttgtaa ttacacgata ctcaccggct tcgaccggta actcccatat ctctccgatt
    17401 gtttgattcc aataaaccag gctgtgatac acccccgcgg aaaattttcc gatcgagatt
    17461 tggctgaagt aattcaaatc cttcccgctt tggtttcgga taatcagtcg ggaattgttt
    17521 cgatcgaccg gttttgaatc ttcaccgatt ccgcaatatt tttcgccgtc gtaaaattct
    17581 ttccggccgg tggagctgtt tattcgcgcg ggaattccca aacttcttgc ggcactgaca
    17641 aataagatat ccaatgaatg ggaatcacaa attccggtag aaaggatatt ttttgcgtca
    17701 caaaccaaag aatcataacc atattcatcc tgaagaatga cagcctttcg taaataatcc
    17761 catagctggg aagctgaacg aatcggttcc gaaaatcggt caaaaaatgc tttcaattgt
    17821 ttccgctgcg gtaaaatcat ttcattttca atccgaggtg ccaaaacgga atgaatatag
    17881 acatcttccg gaaataagcc cttataagga agagaagctt ccaaatattc tttcagtgtt
    17941 tctgaagtga tatcgacaaa gtccttttcg cgcaaagtat tcagaattgc cgctttatcc
    18001 gctgtcatga aggagtcgca ctgtaaaaag tcaattatct cgtcgaaatt tcctttggca
    18061 tccgtaaggt attgcttcac caaagatttg tcccaatccg aatcgagttt gagatttttc
    18121 agcgccggat tttccgcaaa gctcatttcg gtttgcaacc gaacccggtt gcaatgctct
    18181 aaacgtttct catgcatttc tgctgtttcc cgcgaaattt caaccggatt cgataaatcc
    18241 tcagcgggtg gaatcaactc aaaatcaaaa ttccccggtt tgttttcaac ggcttcatca
    18301 aaatcaacgg tgatttcgct ctgccgtctg agatcgacct ttttcatcag gaaatgattt
    18361 ccgtcatgaa cgtgtaaaat aatatcccct ctgccgagca caaatgaagc gaagccgttg
    18421 tcgtcggttt cggcggttaa taacgggtat agttcgctgt aattcacaat ttcacagcgg
    18481 accacggctc cggcaaatgg acggttgtct tttctcacaa aaacattcaa acaggttgtt
    18541 tttgcatacg ttttcaggtt gtttatcata caaaaaacag gagtacgacc ggcgatcaac
    18601 tcacctttca tatccgagtt aaagactttg gaatgaatca acatcgcttt cgaagctgcc
    18661 gcggtaaacc aacctttgtc taaaacgggt tcgggttcac acgcaccgag ataatgccat
    18721 ttcccgtccg aatatgcttc aacccaggca tgattatcat cacagtgagc ccaacgagga
    18781 gtgtaacatt gccttgccgg cagcccgaca ctgcgtaaag cgctgaccag caaggtgctt
    18841 tcttctccgc agcgaccgta tgtccttcga ataacagtta atggtgaagc tgtccggtca
    18901 tccgattctt tataagtagc tttttcataa caccaataat tcacttctaa gatggcatcc
    18961 cgatcggatt tatcccggac acggggccac agttcttcaa agaaccgatt tttataatct
    19021 tcgatgaatt cgttatttac gcgatagaag agaacgaaat tcagaaaaat atcttccgga
    19081 atattcttca gccatggaac ggtattcaac aacatgaccg catgtttcac atgaatatca
    19141 attaattgaa aaggataaga cacaaagtct tgaaccggca tgaatgtata acaaaactcc
    19201 atgcaaattc tttcaacttc cggcaattct ctgataaacc gagctgtttc aactccggac
    19261 ttaccgaaag aaggaagtcg ttgaagataa cctcgatgaa ttgtttgaat tttttcttca
    19321 ctgaaaagca aggtcatttt cctaccgtcc ttccttttcc gtctaaggca gccgttcgtt
    19381 gactcttaaa aataaaaatc atttaatgac ctcatcaata tagcgttgat aagcgattat
    19441 tgatccgccg ataatatgcc cgtctcggcc aaagctttcg aatttcaact ggaacgaatt
    19501 tttaatataa tcgtttaaat gccttcgata atttttttcg atcaattctc ttaacttttt
    19561 attctgttga atcatggacc cgcaaagcaa gatcatttcc ggtgaatagg tattggcaag
    19621 cagattaatg gagatggaaa tataatcgac gacactgtca ataagtttta cagcccaatc
    19681 cgctttctca tcatagcggg caaataaacc ttcgagcgtg atatccgctt cgacactttg
    19741 tgcttccctc aggatagccc attcggcgat ttttgtctga agacaaccga tttgaccgca
    19801 ttcacacatc tggcctaccg gattgatgat aatatgcccg atttcgccgg ccatattatt
    19861 cttaccgcga taaatttcgt tatcaataat gaccgcggca ccgataccgt tcccgatatt
    19921 catcacaacc gaattatggc attgcttcga tgcgccgaaa cgattctcag cgatcgcgat
    19981 cgctttaatg tcattttcaa ccacaaagtc ttttgaagga aaatattctt ttagaatttt
    20041 actgatttga acgttatgcc atttcaggtt ggatgaaaac tccatccttc cttttttatt
    20101 atccacaatt ccgggtaaca cgattcccac caccggtaag atcttccgat cgggctggct
    20161 gtcgatgaat cgttggagat tcattgccag ttgcttcaaa acttcttgcg gatcgaatcg
    20221 actggaagtg atcgtgtatt cccgaatagc ttttatctct ccgaaaggat cgaccaatcc
    20281 cacccgcatc gtgtcctttt caaggctgac actcaatgaa agcaggttgt cgcgattgag
    20341 agaaagcatc atcggaggcc ttccgattga attctccttg gtgcggtccg tctcacaaac
    20401 gaaacccaat tggatcagat catcggtaat ccgattgatt gacataatgc tcatttgcac
    20461 tttttcggca atttgcgctt tggagatttc atcagcctca ttaatacagg ccaacacgag
    20521 cttcatatta tttttcttca tcagatcttt atctaatttc tgcgttttca tcatgaaggc
    20581 ctctttaaat atcactgatc ggatcattca ttacggtttc aatataatta ttatcgtcat
    20641 gttaatatta aagctgtaat acttcgtgct tatttctatt ttatcaaact ttcaaaagct
    20701 ttacaaaccg atttttacca aaatttccgg ttacaaagta taatgacaca aaactagaag
    20761 aataatattg caagcgaggt taataatggg gaacattctt gtcgaagtct gctgcggcag
    20821 cgcagatgat gcggttgaat cttttcaggc gggagcggat cgagtcgaat tgaacagcga
    20881 cctttttcac ggcgggttaa ccccttcgat cggcgaactg atcattgcaa aaaagcatac
    20941 cggcttgaaa atcatgacaa tggttcggcc cagagaagga ggattctgct ataccgaaaa
    21001 tgagtttgcc acggcggttg aggatgcaaa attattatta gcccacggtt cggacggatt
    21061 ggtgttcggt tttctcaaat cggatgggac aatcgatgtt gaacgaacaa aaattatgaa
    21121 gcaaattgcc ggcgataaaa caaccgtttt tcatcgggca attgatgttg tgccggactg
    21181 gaaatccgcc ttggataccc tcatctcgct tggaataacc agggttctga ccagcggaca
    21241 ggaatcgaat gtctttttcg gaatcgaaac agtaaaagaa atgatcacct atgctgacgg
    21301 aagaatagaa attttgccgg gagcgggcat aacattgaaa aatatcgacc ggatcgtcag
    21361 tgaaaccgga tgcgatcagg tacatgttgc ccattacaaa ccgtattcgg attcttctgt
    21421 caataataat cgcgaaatat tctacggcgg cgcattatat ccgccggaag atcgtttcaa
    21481 tatggtggac agggattata tttccgccat cgttaatatg acaaactaaa aatattcagg
    21541 cagtataaat atttttcagt ttctcctcca aaccatgagg gcgaatcgcc ctcatggttt
    21601 ttaatcccgg atcaaactcc gcatcgaatc gatatttatt tggataaaac agtccggcga
    21661 tatcgcttca aaggataaat tccatattat cgagtcccat gatcaatccc ggataaacct
    21721 ttcttcccgt atccactgcc cggctccgtt tatcgatcgt ttcaaaggcg tttgcgccga
    21781 aatgaatcaa gacagtctgt tttgcgtttg aatcagacgc caatttcgct gccgtttccg
    21841 gcgtcatatg acccgatacc gtattgacgg ttccgggata aaaaccgcat tccgtaatca
    21901 gcaaatcggc gttttttgct aatttgaccg cattttcgca gtaagcggta tcaattacga
    21961 aggcaataat ttttccatcg atttcaaatc ggtaaccggt atccggtacg gcatgtacca
    22021 gaggcaaggc ttcgactttg aaaggaagat tcgttttttc caaattcttc gcatcaatca
    22081 tctcaatcgg ataaacatgg ctatcccatg ctgcggaaaa aggggatcgc agcaagtctt
    22141 tcagatcatc cgttcctccg atgggcgtaa tcactcgcaa cggttgttcg aaattgaaat
    22201 aatcgagcgc atgtaacccg atcacatgat ccaaatggag atgggtcaga aaaagataga
    22261 ccggttttga aaaatcgact ctgtttttaa tttttgtaat gccaaagccc gcatccatga
    22321 ttacggaata atcggatgtc tggagcagca cacagtttgt gctgccggta tctgtatcaa
    22381 accaaccgtt tgaccctaaa acagttattt tcatgatttc actcctaagc tatttctgca
    22441 tagaatttta catcaagatt attcacggaa tttgtccgcc tcgaaaagca gagtataatg
    22501 gtcaataata agccgaaagc gaacgaaaga catgattccc gaaatcaaag acctttgcac
    22561 atttgccgaa caggccggag tgatcaccat tgatggattt ggtaaaacac accaagtcac
    22621 tcaaaaagga atccaggatc cggttaccga aatcgatcag aaatccgaaa attttcttat
    22681 tcgacaaatc aatcatttct atccggggca taccattctc agcgaagaaa gcggccttca
    22741 taacggaacg ataaccgctg cggaacatgt ttggtatttg gacccgttgg atgggacaat
    22801 gaactattca cacggcgtac ctttttacag catctccatc ggttatgctt tcaaagggga
    22861 gctggtattg ggagttattt acgatccgat gcgacacgaa tgctattgtg ccgaaaaagg
    22921 aaagggtgcc tatttaaacg gtacaaaaat acaggtttca cgggaaacag atttgtgcta
    22981 tgccttgctt gccacgggat ttaacatgaa gattccggaa acgggaagag ataatttccc
    23041 tctgttcaag cattttatgc aaactacagc gggtgtacga aggatgggtt cggctgcttt
    23101 ggaaatagct tacactgcgg cgggcaggct ggatggaatg tgggaaatgc atctatcggc
    23161 atgggatgtc gccgccggat ttgttatcgc gcgggaagcg ggcgctgtgg tcagtaatct
    23221 caaaggcgat ccgaattgct ttcatgtacc ctttgaattt ttgatcgcca atccgatcct
    23281 tcatcctttg ttgttaaaag aaatcgaact gatgatagaa tcgaaacaga cagaatgaaa
    23341 attgatcgaa cgtttttgcg aaatctgatt ctctatggaa ttatcggtgc gatcagcgcc
    23401 ggttttgatg cgcttgtttt ctatctgctt tttgacaaat tggcgatcaa caaatttgtc
    23461 gccaatgtaa tcagcgttca tttcggcatt tttttaagtt ttttcctcaa tagtttttat
    23521 aacttcaaga aaacagacaa aatgggcaag cgattcttct ctttctatct gacaggatta
    23581 ttcgggctcg gcttatccac attgattctt tacgccggcg gaaggctgag aatcgatgtt
    23641 atggcaacca aagtcttttc cgttgttttt gttgcgctgg tccaatttat aatcaaccga
    23701 gctgttgcgt tcggcgacaa ataaaaataa tttgatgaaa ttcatttgag aaattttcat
    23761 gaacagaacc gaaatattgc tcggtatact ggcgggatta tttatttcac agaagatccg
    23821 tgtcccctta ccgatatggc tgatattttt gcttttgttc gctgttttgg ctgtattctg
    23881 cggaaagaaa tcttcaaaat acgtccttga tcaaccggta aaaaaagcgg cgatgattgc
    23941 tttgacggct gctttctgct gcctcggcgg attgcgcgga tcagtgacaa atcaacccct
    24001 cccttccgga catttatccg aagtctccgg tgaagaagtt tcctttaaag ggatcatcaa
    24061 ccgtcccgtg acacgatcgg attcacagac acggttgcgg attcgtttgg ttcggaatga
    24121 agattcggat tctgaccccg atcggggaga atgtattgtg gttctcaatt ctttgacaaa
    24181 ttcttttgaa tatggggatc ttctttccgt ccgcggaaaa ttgacaaaac tcgacgcctc
    24241 tgccccaaat tcttacggaa gttatctgga gcgatcgggt atatccggaa ttttatataa
    24301 tcccgaaatc gaattgattg caaaacaaca ggggaatccg attctcaata gaatctttcg
    24361 gcttcaatcc tatttgctgc aaaaaactta tgatatattc ccggaacctg aaaattcgtt
    24421 gatggcgggg atattgttgg gagataagag ccgtattccg gcggaaatcg aagaagcatt
    24481 tcgaaacacg ggaaccgctc atattatcgc aatcagcggg atgaattttt ccgttttgat
    24541 ctggatgctt ttatccgtct tacgagcgtt tcataaacgc tggtggacac cattaatcct
    24601 attgccgttt attttcttct acacgattct taccggggcg aacccggcga tcgtccgagc
    24661 cggaattatg ggaggaattg ccttgatcgg aatgagcatc ggcagagaga aaggaggagt
    24721 tgattcattg cttttatccg tcgctgcgat ggcgatgttt caaccgaaaa ttctttttga
    24781 cgtgggcttt cagttatcag ctacggcaac tctgggaatt ttgattttta atccgccatt
    24841 gaatagtctg atcgaaaaag gatttacagc aattcatctg aaaaataatg tccaaaaaat
    24901 tcttctggat acgctgaacg aaatattgat cacttcattt tccgctcaga tatttactac
    24961 ttggattatt gccgcatcat ttcatcaatt cgcaatattt tccctgattg tcaatatgct
    25021 tatcgcgccg tttcaaaacc tgatcatggt cggaggtgtg tttgcgttat tcctttcaat
    25081 actatccgaa tcgctgggaa aaattgccgg cctaatcgtt gatcctttac cggcattaac
    25141 catccgaata atctgctttt tcggaaaatt tgactgggca tctcaataca gtgatctttc
    25201 aaatttggct gcctggatca ttattttttc gatcctgaca gtttggatca atcgaaatcg
    25261 ctggattcga caaaagaagg aaactttaat ccaagccggc ataaccattc ttgcgctttt
    25321 tacacttgtt tgctggcggc aggttttggc aaaagcggat caccgacttc atctgacttt
    25381 taccggcggc accaatacaa gctctatctt gattcgcttg ccggaatctc aaaaaattct
    25441 ggtggcaaac ggagttacaa attttcaagc ccaaaatctt ctgaaaaaga acccctttga
    25501 cccggcagat tatgccgctg caattttgga tttccaagaa gaatggatgg cagacgaata
    25561 tctgaggagt gatgatgaaa agcctgaatg gttgattctc aacggtagga atcgcagttt
    25621 atccgccgaa caaacaaacc caacgcctgt ctatttgaac gaaggatttc aactccgaac
    25681 gaatccgttt attctcactt tgcagcaaga atatctcaag cgaaaaaggt ggataataag
    25741 ctaccgatct ttgacaatcg atatcccaaa cggtatcccg cttcatcgaa tcgacacctt
    25801 gaacgatccg aacccttccg tttcaatcat tctgttaggg aagaaagaca atgaaaggga
    25861 atgggaagaa tatttatcac aaacttattg tcatggttcc tcctgttcgg ggaaacctat
    25921 tttattgagt acgaatgaat acggaaccat cgaagttatt tccgacggcg aaaaaatctg
    25981 gctggttcag caatcttctt aaccataagt ttcgtatcga tcccacagag taatgagaaa
    26041 cgtcgattta ttaattcttt ccataaaatt tacaaaaagc cgaaaactct tacaaaaatt
    26101 tactctgtaa acagaccctt taatcgtatt ttttgataga atcaatctat acaaaagaaa
    26161 gagtgaaggg aggataatat gttgcccctt gataatgtat tggcagtcat tctcggcggt
    26221 ggccgaggga ccaggttgtt tcctcttacg caattgcgct caaagccggc ggtaccgatc
    26281 gcaggtcagt accggttaat tgatatcccg atcagtaatt gcatcaattc cggaattttt
    26341 aagattgcgg tcttaaccca attcaactcc gtctcccttc acagacacat cttggatact
    26401 tatcagtttg atcggtttca ttccggatgg gttcaaattt gggcagcgga acaacggaca
    26461 gataacaatg attggtatca gggaaccgca gatgcggttc gaaaacaatt gagtgagatt
    26521 atctcatcgc gaacggaata tgttctgatc ttatccggtg accatctgta tcgttgtaat
    26581 tacgcggata tgcttcaatt tcatttagat caccatgcgg atgtaacgat cgccactcaa
    26641 cccgtgcata aggatgaagc ggatcgcttc ggtattttga agcgtgattc cgaattcaaa
    26701 atcaatgatt tcaaggaaaa acccaaagat cccgaagttc ttaaggaatt ggtcagtctg
    26761 gatgacgaag aacgacctta tctgggctct atgggaattt atctgttcaa taaagatctg
    26821 ttggtcggct tactgaatgc actggactat tccgacttcg gcaagcatat tatcccttat
    26881 gcaattgaaa aatatgatgt ttacggctgg acctttgatg actactggga agacatcggg
    26941 acaattcgct cattctatga aacaaattta atgctgacgg aagatcatcc gaaattcaat
    27001 ttttacgatc cgaagcatcc gatttatacc ttaaacaaga ccatgcccac gacgaaaatt
    27061 gaaaataccg aggttaaaaa tgctctgatt gcgaacggct gcatgattaa agcgaacagt
    27121 atcgaacatt ccattatcgg tatacgcagt atgattgatg tcgggacaac aatcaaagat
    27181 tctattttga tgggcgttga tgatgatgtc ttcgaaaaga gtgccaacga ctcaaagtat
    27241 tttgatatcc ctttgggaat cggaaaaaat tgccatattg agcaagctat aattgataaa
    27301 aatgcccgta tcggtgataa tgtcatcatt aaacccttcc cgccccatat tgaattggac
    27361 gatgcggatc ccattaagaa atattatgta cgtgacggga ttgttattat tcccaagaat
    27421 accagtataa aatccggcac ggtaatcact ccgtaaatac aacttttgat cagaaaaaac
    27481 ggacgcttga agcgcccgtt tttttctgaa atttatttca ctcaaataac tttaattcgg
    27541 aagcgatggg atgaaatgac ttccgatgat acttacaggg accgaatttt tccaaagcct
    27601 cttgatgaaa ctgggtaccg taccctttat ggtgttcgaa tccatattgc ggaaattctt
    27661 cggcacagtg ttcaaccatc caatgatccc ggcaaacttt tgccaggata gaagccgccg
    27721 cgatactgta agagcattca tctcctttga tgatggattt ctgcggcatc atttggcgga
    27781 ataatcggac ataatcaatc agtaaatact gcggcagcgg ttgaagttga gcaattgctc
    27841 ggtccattgc cagtcgtgta gccggcaaaa ttcccatgga atcaatttcc caatttgaag
    27901 cggatcccac tgcccagccg atcgcataat cggtaattat ccggaatttt tcttcccgag
    27961 tttccggcgt catttgttta ctgtcacgca cgccgtccaa aataacaggc agattttcat
    28021 ccaccgggaa aatgaccgct cccgccataa ccggaccggc ccaagctcct cgaccggctt
    28081 cgtcgattcc cgccacataa aaaaagcctt gttcccacaa agctttttca taatcgagag
    28141 tcggattttt cttcattgtt cgatcttata aattccggat ttatcgttgc agcgtatttg
    28201 caatagttcc cgaaacatct tgactgaatc attcagcaca tgaactttgc tttgtccgtc
    28261 ataataccaa cgtaccggaa cttcgacaat tttaaatccg tttttttggg ctatatacaa
    28321 cacttccgca tcaaatgccc aaccgtctaa aacctgaacc gagaaaattt tttctgccgc
    28381 gtttgctgta aaacacttga aaccgcactg agtatcctgg atgttatgta cggtcattag
    28441 acggacaatc tgattaaata ctcgaccggt caggtgacgg atcggtggtt cattatagcg
    28501 aattgcccct ttaacttctc tggatccgat agcgacatca taatcggaaa gcttgggagg
    28561 aaggaaattt aatatttctt caatgggcat ggagaaatca acatcggcaa aaaaacgata
    28621 ttcccccttt gcagccaaca tacccgttcg aaccgccaac cctttgccgg gttttgaatt
    28681 ttgcaaaact ctcaaccagt catatcgttt tgcatattcg gttgcgatag ccaatgtctg
    28741 atcgacactg ccgttttcta ccacaatcac ttcaaagggg aaccggagag aagaaacaaa
    28801 tgcgattacc ttttcaagac aaatgggtaa gcgtttttct tcattatggg ccggaagaat
    28861 aatcgataaa taaatatgag aggaggggtt tatcgtactt tccattggct tttcccctaa
    28921 atcttatcat aaaaaacata aaatcgaaga gtcgtttggt tttacagtca ggggaaagaa
    28981 gggtcatctg tcggggtgcg cggacttgaa ccgcggaccc ccgcgtccca aacgcggtgc
    29041 gctaccaact gcgctacacc ccgaaaaagc aatacgagta tactctttct gataaaattt
    29101 cgcaagaaaa acaaaaattt gtgttgttat tcaaccataa tggtgtgtca aggctattta
    29161 caaatgtcct aatatcgacg c
//
LOCUS       DAOSMM010000022        29142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1614_length_29142_cov_16.030873, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000022 DAOSMM010000000
VERSION     DAOSMM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 29142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29142
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1614_length_29142_cov_16.030873"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..935
                     /locus_tag="PK898_04985"
     CDS             54..935
                     /locus_tag="PK898_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU19423.1"
                     /translation="MKKNLDILMVGHFAKDKIVIDGNEFESPGGAVYYGSVVLRRLGL
                     EVGVATRGNPDDFHYLKELTDLGIEVDAKPAPQSSGCINIYQSADMERRICKLNGFAG
                     EIPYEDIPDVNAKIIMVTPIIAGEITLPTLIKLSEKAPIALDVQGFVRVPEGNDLNFR
                     PWKDMEEGLKHVTFLKVDKAEAEHITGTDDLEKAAVILAGYGPKEIVLTQSKEVLVYA
                     DGKFYRAPFTPKSLAGRTGRGDTCITSYVGSRLKYDPERSCKIAGIITTMKQEKHGPW
                     TGEIDPQRFDPSIVFGD"
     gene            985..1569
                     /locus_tag="PK898_04990"
     CDS             985..1569
                     /locus_tag="PK898_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19424.1"
                     /translation="MAEKKVSRKKVNMNAASSNEEENLESGTKEQSLNTAETNNSLPQ
                     AEENEIKGASESQSNIIESPANIHQDFFEENKKVEEKKMEENEAAAGSVNPVPEFEKL
                     PDPFIQEPEISDKKIPAENEGIEKDASEEGKTVEIPETSPSGELPKDVFEEGTGEEKK
                     NNNNIWLWIVIAIIVIIFLCFCGFIGSIVFLFWG"
     gene            1664..2023
                     /locus_tag="PK898_04995"
     CDS             1664..2023
                     /locus_tag="PK898_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19425.1"
                     /translation="MKENNPIVYSTEKGRICPECDFPEKECHCRTKKISKIISDDGTI
                     RIRKEKKGRNGKEVTIIQGISLSEQAVQSIVSDIKKKLGTGGTVKDDIIEIQGDRVKF
                     IAEYFIKLGYKVQINGG"
     gene            2025..3710
                     /gene="ettA"
                     /locus_tag="PK898_05000"
     CDS             2025..3710
                     /gene="ettA"
                     /locus_tag="PK898_05000"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOU19426.1"
                     /translation="MAVEQNKIIYSMIGVSKYYDKKVILKDIYLSYFYGAKIGVLGLN
                     GAGKSSLLKILAGVDKDFIGETVVAPGYTIGYLEQEPLVDSKKTVREVVEEGAQEAIN
                     LLKEFEEISNKFSEPMSPDEMDRLIEKQGKIQEKIDALDAWDIDSRLEMAMDALRCPP
                     GDSPVDILSGGEKRRVALCRLLLQKPDILLLDEPTNHLDAEAVAWLERHLQQYKGTVI
                     AVTHDRYFLDHVAGWILELDRGQGIPYQGNYSSWLEQKKIRLEQEEKGESQRQRTLAR
                     ELEWIHMSPKGQHAKSKARISSYEKLLTQDFTKANEELQLYIPPGPRLGNLVVEMKDV
                     QKVFGNRIIMDHVTFTLPPGGVIGIIGPNGAGKTTLFRMIVGEEKPDSGTIRIGETVK
                     LGYMNQSRDSLDPEKTIWEVISEGQDEIKFGDRKMNSRAYVARFNFSGADQQKKVGVL
                     SGGERNRVQLALTLKSEANVLLLDEPTNDLDVNTIRALEEGLENFGGCALVISHDRWF
                     LDRIATHILAFEDDSSVIFFDGNYTQYEADRRNRLGIEADTPHRIKYRSLTRI"
     gene            3968..4987
                     /gene="iolG"
                     /locus_tag="PK898_05005"
     CDS             3968..4987
                     /gene="iolG"
                     /locus_tag="PK898_05005"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HOU19427.1"
                     /translation="MNRIRVGIIGAGRIGRVHAQSIAFGIPEAEIVAISDPFIENAER
                     AARDFAIPAIYADYHAILNDPTIQAVLICSPTNTHADIAMEAARAGKHIFCEKPVDQT
                     VSKILETKKLVEENAVKMQIGFNRRFDHNFRKIKKLRDDGTLGEIQIIRITSRDPEPP
                     SPEYAKTSGGMFLDMTIHDFDMACYQANSEVDEVYAMGAVTVDPEIGKAGDIDTAIVT
                     LKFKNGAMAVIDNCRKAVYGYDQRVEVFGTNGAAASENDRETMVKISSKDAVCEDKPL
                     FFFLERYMQSFRDEIKSFINAILNDRPVEVNIDDGLRPILIAIAANKSMKENRPVKIS
                     EVMEG"
     gene            4992..5804
                     /locus_tag="PK898_05010"
     CDS             4992..5804
                     /locus_tag="PK898_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOU19428.1"
                     /translation="MSKKIIKGKPFDFGYNPITEIGGDPDMLMDFGILRLKSGQIYED
                     NSALERAYLLVYGEITLEYNKSSIKINRPNCFDYAPWVLHLPADVPVRITGVAEDSEI
                     TVNKTVNSRIFDPKLYEPKDSPDEFRGAGTMQETSTRIVRTTFDYNNAPYANLVIGEV
                     IGFPGKWSSYPPHFHPQPEIYYYKTYPTGGHAFAEVGDDVFKISGNDTVLIQNGMTHP
                     HVTYPGYALWYLWAIRHLDGNPYITPTFVPEHEWVAKPDAKFWSYGNKKEMK"
     gene            5801..7669
                     /gene="iolD"
                     /locus_tag="PK898_05015"
     CDS             5801..7669
                     /gene="iolD"
                     /locus_tag="PK898_05015"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HOU19429.1"
                     /translation="MIKRIRLTMAQALIKFLDNQYVEFDGEEDRFVEGMIGVFGHGCV
                     TGVGQALEQGGHSLRFYQGHNEQGMAHVAIGYAKQKNRRKILPCLSSIGPGALNMVTA
                     CGTATANRIPLLVLPGDTFACRQPDPVLQQVEFPDSYSTTVNDSFRAVCRYWDRISRP
                     EQLMTAMIHAMRVLTDPADTGAVCVALPQDVEAEAYDYPEYFFRKRVWHMDRRPITDR
                     QLEQAIDLISNSKKPLLICGGGVRYSEAWKDLQKFAEEFNIPIGETQAGKGLIAWDHP
                     LNLGGIGVTGTKAANLIARDADLIIGVGTRFTDFTTNSKWGFRNPECKILAINVCSFD
                     AVKMDAEGILADAREALSAIRKELKNLNYQSKYTDEIIEAKKEWDDIVNEYYNLPEEK
                     NGIPQTRALGEINRFMRSKDIAVGSAGSLPGDMQRLFRPGKPGTYHMEYGFSCMGYEI
                     NAAVGVKMAAPDSEVYAFLGDGSFIMLHSELFMAIQEGLKINIMVFDNSGWGCIESLQ
                     NNQGTKTFGTVFRARNPETDLLDGEILPVNFAKIAEGYGAKGYTIHNADELKKALKNS
                     KMQEKPCVFDIKVLPGSMTPGFESWWRVGVAEVAEDQSVNEAYAEMQKHIAEARDY"
     gene            7681..8577
                     /gene="iolE"
                     /locus_tag="PK898_05020"
     CDS             7681..8577
                     /gene="iolE"
                     /locus_tag="PK898_05020"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HOU19430.1"
                     /translation="MLNPEKVKLGIAPIGWTNDDMPDLGAENTFEQCISEMALAGFKG
                     CEIGNKFPSDPAVLKHKLEVRGLQICNAWFSSYLIGRPYEETIASFIRHRDKLNYLGA
                     KIIGASEQGNSIQGKIDVPILENKPIYTEEEWKIVTKGFNEMGELAREKGMFFTVHHH
                     MGTGIQTEEEIDRIMEMTDPNLVYLLFDSGHLSFAGIDPVHILKKYIHRVKHVHLKDL
                     RYNVYAEVKAKRMSFLDAVRAGVFTVPGDGDVDFKPIFDILEEAGYEGWIVVEAEQDP
                     AKANPFEYAVKARKYIRDITGL"
     gene            8599..9621
                     /locus_tag="PK898_05025"
     CDS             8599..9621
                     /locus_tag="PK898_05025"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOU19431.1"
                     /translation="MVKVGVCGYGVIGQRLADGVALQKDMELVGVADVAPTLAIRALK
                     EKGMPYALYLASEENRAKFEQLKIPVSGTLEELVRKCDIILDATSAGVGAKNKELYAK
                     YNKKAVFQGGEKNSVADVFFHGYANYEKGIGKQFLKLTSCNTTGLIRAVDCLDRDYGI
                     KKVAITIVRRVADPGDYHRGLTNALQIDKAPSHQAVDLMTIMPHIDATGILIHTPVTH
                     GHIITVVATGHKKITKEMALESFLKHDRIRVVSTDDGFLGNASFFRYARDLGNPRGDM
                     YEIGLWSDSIVESGDEIMFAINIPQESVTIPETMDAVRAACEMQTDRLEGIGKTNTYL
                     GMGKWK"
     gene            9612..10874
                     /gene="pgk"
                     /locus_tag="PK898_05030"
     CDS             9612..10874
                     /gene="pgk"
                     /locus_tag="PK898_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU19432.1"
                     /translation="MEIKMLSLEGVDLRGKKVLLRPDINSPLDPQSKEIVNDERIVKT
                     IPVIQMLLKGGASVAIIAHQGDTLDYQNLTDLSKHAQKISEYLDHPVEYLDDVCGPAA
                     VQRVKALKPGEIVILGNLRYLTEEVSTFERDVKLDAAAYTKTWEVRNLAPLFDLYVNE
                     AFSAAHRNSPSMVAFQELLPTYAGPLLFQEYEALSKILHGAQKPAVFVLGGAKISDAF
                     GMMKPVLENGTADKILTSGVTGLVMLIAAGINVGEKTVRFLKDKDLLGFVEDSKEYLK
                     NYPDKIMMPVDVAIEENGKRVEIDIDEMPKDTLYLDIGAKTVAAYREVLMHAKTIFAN
                     GPAGVYENPLFEAGTKGLWQAIADSPAYSVIGGGDTISSATRLIDISKIGYVCTGGGA
                     MVRFMSGKKLPLISAMEKAYERDQSRTI"
     gene            11057..11884
                     /locus_tag="PK898_05035"
     CDS             11057..11884
                     /locus_tag="PK898_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU19433.1"
                     /translation="MITPLKEFATDIRIQEMIEFKARGFGHTGGSLSVADCLAALYGG
                     IMRIDPKNPQWEDRDRLVLSKGHAGPALYATLALKGYFPLEELKTLNQNGTKLPSHCD
                     RNLTVGVDMTTGSLGQGASTACGMALGLKHSGKDARVYLILGDGECQEGQVWEMALFA
                     AHHKLNNLIAFIDYNKRQLDGYVKDICDLGDLVAKFKSFNWYTTMIKGDDPDLIRQTV
                     LDVQKNQGDRPGMIVLDTIKGAGVPQIENIPLNHHTNVTPEEADEIIAQLEEFKRKG"
     gene            11891..12853
                     /locus_tag="PK898_05040"
     CDS             11891..12853
                     /locus_tag="PK898_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU19434.1"
                     /translation="MFKLIYNGEKEKKAGKKVFGETMQKIVEQDPLAVYLDGDIMNSI
                     GTLGLEKKFPDQVFECGIQEADMMGIAGGLSAVGKKPYVHTFAAFAARRAFDQVFISI
                     AYAKNNVRIIGSDPGVTSAYNGGTHMPFEDICLYRAIPHSTVLEFTDSAMLASLLPTL
                     KDRPGLTYCRLNRKEGTAIYSEDSTFEPGKANLLRDGKDVTIVACGMMVGESLKAAEI
                     LEKEGISAAVIDMFTIKPLDEEIIKEFAGKTHAFVTAENANIVGGLGAAVAAFLAETT
                     PCPVIRVGVKDEFGQVGTPEYLAEVYGLTSKEIIEAVKKAVSMK"
     gene            12882..13919
                     /locus_tag="PK898_05045"
     CDS             12882..13919
                     /locus_tag="PK898_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU19435.1"
                     /translation="MDCGDPFLISMADVTIKDIAEATGVSYATVSRTLNSRNGVSPKT
                     REKVLQTAKEMGYKPNIHARSLKTNQTLTIALIVPDISNPFFADIALSVNKTAFAKGY
                     NTILCSTNWDPEVERLQLMHIQEQRVDGIIFKPCDRCTDRYMDIHIPKVMIANLLKTE
                     GLSYIEVDNILGGQKAASHLIACGYRKPAFLGGEIVSNSNADRLFGFQSTFQTFGIEI
                     PDERIHFGPFTIENGYQSAKRQIELSSSPTPDCFFCGNDLIALGAMQYLAEQRIRIPE
                     DFGVVGFDDVYFASLPQIQMTTIAQPRNQMGVLAAEILIRHIESGDNEEEHILLQPEL
                     IIRNSTRKPLR"
     gene            13944..15197
                     /gene="larA"
                     /locus_tag="PK898_05050"
     CDS             13944..15197
                     /gene="larA"
                     /locus_tag="PK898_05050"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOU19436.1"
                     /translation="MNISFGFGQSTVQLNIPDKNLLKVLEANPIQMIGDESEIVQKAL
                     QNPIQSRRLNEIVRKGEKIAIITSDITRPMPSARVLPFVLDELSSAGIPPSDIVIIFA
                     LGNHRSHTEEEKKMLVGEEIFKKYECIDSDGPMVRLGITANGTPVDIFERVVKVDKRI
                     CLGNIEYHYFAGYSGGAKAIMPGVSTREAIQANHRHMTDPNAIAGKIENNPVRTDIES
                     VLNFISVDFIVNVILDEHKKIVRCVAGDVIAAHRQGCRYLDQMYSVEIPEKADIVLVS
                     PGGYPKDINLYQAQKALDNAKNAVRNGGSILWIASAKEGLGEKTFEEWMLHHNNPSEM
                     IDHIQKEFVLGGHKAAAIAMVMQKATVILYSELNPDLVRKIKLESTQNPQETLERLIN
                     QYGQNASIIAMPYGGATLPILADKR"
     gene            15201..16094
                     /locus_tag="PK898_05055"
     CDS             15201..16094
                     /locus_tag="PK898_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU19437.1"
                     /translation="MKYYGAIEAGGTKFVCSILADPDHYFEEVRFPTTTPEETLERTV
                     SFFKEQRNKYELTAIGVSCFGPIDLNPSSPTYGYITTTPKIKWRYTNIVGFLKQELNL
                     PVGFDTDVNGAALAEYKWGNPENIRNLVYYTVGTGVGGGVVIDGKPVHGLVHPEAGHI
                     LIRQNLEKDPFKGDCPYHGNCLEGLCAGPTIQARFGIPPQNLPEDHPFWPILANYLAQ
                     ACIAQIYQISPQKIVLGGGVMGQKQLYPMIRKEVIRLLNGYIQHPMILEHIDQYITPP
                     ILGNRAGACGAMALAQMLEKQ"
     gene            complement(16098..17825)
                     /locus_tag="PK898_05060"
     CDS             complement(16098..17825)
                     /locus_tag="PK898_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU19438.1"
                     /translation="MYGIRDAQKEFDRARNQAIWQQALGFLQRKNYNLIDYDEVRKRL
                     AKTVNALPVQKDIPIDAIVGSVSRNNDFTRTFLPKMDYDKNRWVNVRLANESMEGVPP
                     IDVYQIGEVYFVLDGHHRISVMKSYGAQYINANVRIINTEVPLKPTDSPDEIIVKTEK
                     QAFLDQTNIEKLIPEANLEMYLPGEYPELAEHIAVHRYFMGLDYKRDISKDEAVMDWY
                     RTVYMPVIEVIRSQKIMDEFPGKTETELYLWIENNKAALTEEYGEGLRMTALAWKLDE
                     EYGKQKKSIGYRFKKAFFMIFSPDLSDWGIKTGDWRKELRKDDSSLSIQRMMISVRDP
                     IEDFDYLQSAVCFCKQFNAWAGIVHVVKRPSMLDSEWIRKYEQDIQTLLEKENVKGKF
                     FVLKGNLLKNLSDRAFWSDISLFKMNYRPSPSRYRSLGSGWNSIFMRVPGPIFVTPDL
                     FPSKIQQILLAFSQSPKSREAMYFASGLAQSTGGHITVVVSGGDEQRRAVTRQEVENY
                     YQAQGIQAEYVTSSDEPGKAIIENANRKKSDLIIMGGYSRSYIKRLFTGSTIEKVLSV
                     TSIPVIICK"
     gene            complement(17951..18682)
                     /locus_tag="PK898_05065"
     CDS             complement(17951..18682)
                     /locus_tag="PK898_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19439.1"
                     /translation="MVTIENLSVEYEDGTFALRAINLKVHDGDSIAILGENGAGKTTL
                     FYTMLGLLKISEGKILVNDIELSSPTLKDIRSQIGLVFQNPDDQLFMPTVEEDIAFGL
                     RNYGKSEEEINKKIRDIAERLGIVHLLKKAPYKLSGGEKRLAALATVLAMEPQAILFD
                     EPSSFLDPRSRRTVINLLKEIRDTKMIATHDIDLAWELCDRAIILSKGEVVAEGSIKE
                     ILTNENLLMKYGFELTTCSQTDSRF"
     gene            complement(18695..19447)
                     /gene="cbiQ"
                     /locus_tag="PK898_05070"
     CDS             complement(18695..19447)
                     /gene="cbiQ"
                     /locus_tag="PK898_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOU19440.1"
                     /translation="MANGNTVIHKLHPYVKSVVLVFYLITVLSVSPYQFDRLAPFILF
                     PAVAISLAEIPFRFIFQRILPVFPFVIFAGLSNLWFDRRIAFFMDSLPITYGAVSFIT
                     LILKTFLTVSAVLILLTTTPMKSLTDLLRSIKIPELIITQIMLSYRYITLVVDEVSVM
                     ETAYLLRSCGKQRIELKHIGSFIGQILLRSYDRAARVAFAMKCRGYGNISNISSSNPL
                     KPVDILIGMGMIGLIFGLRVWDISGVLSRLFL"
     gene            complement(19546..20559)
                     /locus_tag="PK898_05075"
     CDS             complement(19546..20559)
                     /locus_tag="PK898_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOU19441.1"
                     /translation="MTDALINPAVGGMMWAGSAGAIAYSVKKIKESDSEEKTAPLMGV
                     MGAFVFAAQMVNFAIPGTGSSGHIGGGILLAAVLGPFPAFLTLSVVLLIQALFFADGG
                     LIALGCNIFNMGVIPCFLIYPLIFQPIIKRGHTTRNFFLASVISSTVNLEAGAFCVVV
                     ETLLSGITALPFREFLLLMLPIHLPIGLVEGVITASVLIYVFKVRPEILHFLKKEEQP
                     NKVPLRLILASFSIVTILTAGFFSLHASTNPDGLEWSIAQITDSTEFNEEDSLPNKSA
                     EIQHQHSVLPDYNFKEGKTLGNINGTTVAGFLGSGLVFVIAGVSGWLISKLKKRKRES
                     NIE"
     gene            complement(20722..21135)
                     /locus_tag="PK898_05080"
     CDS             complement(20722..21135)
                     /locus_tag="PK898_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HOU19442.1"
                     /translation="MKIVVIDGQGGSIGKTIIEQLKKNSFSDPIIAIGTNSIATSTML
                     KAGADFAATGENPILVACEDADILIGPMGIIAANSLLGEITPAIAVAVGKSKAEKILI
                     PSNKCKITVAGMHDLTLGEAINQAVQTVLNYKNEK"
     gene            complement(21236..22714)
                     /locus_tag="PK898_05085"
     CDS             complement(21236..22714)
                     /locus_tag="PK898_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19443.1"
                     /translation="MTKKVLFNDLTLRDGHQSCAATRMTTEQAMRVLPIIKNAGYNMF
                     ELWGGATLDSAIRFTKDDPWERLERFRDEMGGGDKIQSLLRGQNLFAYSPYPDDLLAA
                     FIRQSIESGSKNMRIFDAMNDVRNLWMAILATKAYGGKVEAAMCYTTSPIHTTQYFVD
                     YGKRLLEEGVDRIAIKDMAGLLHPKDAVVLFPALRKEINVPIVLHTHTTTGVALVNLA
                     LGMKYGLDQFDTCISPFAGGPSHSPIEVMAVVAERMGFEHDLDKDAISKAQDVLRVIF
                     EELKGSIPSFGKSRRKPVYFDDVPVDKVDKMVEAIMKTPEDLDTAIAVSREILADLDY
                     PGYDDKVFITQIPGGMISNLQSQMKEMGVTGDMMDAVMEEFPKVRKDVGYVPLVTPTS
                     QIVGSQAAFNVLAGERYAFVSNEFKMILRGEFGKTPGPIDEEFRKKILGDDVEVKKYR
                     PASYLMPVLEDEVNLPYIKSKKDLLLHHVFGNAADEFLKSRK"
     gene            23236..24369
                     /locus_tag="PK898_05090"
     CDS             23236..24369
                     /locus_tag="PK898_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOU19444.1"
                     /translation="MKKQIDLQQFPAGNLPARILMGPGPSMVDPRVYQAMAAPLVGHL
                     DPEFVHLMDRTQELLRYTFRTENKLTIPVSGTGSAAMEASIANSVEPGTPVLICINGY
                     FGERLADMAKRYGGDVSTIHRPWGEVFTPSEIEQALREKPAKVVAIVHAETSTGAEQP
                     LDEIAKIVHNHGAILIVDAVTSLGGVPLSVDEIGIDICYSGTQKCISAPPGLGPITFN
                     ERALNLMQSRKAPVTSWYLDMSLVQAYWGPNRTYHHTAPITMNYALYEALRIIAEEGI
                     ENRWARHRANSEMFWEGLEDLNLKCFIPKEHRLTSLCTIQIPEGVDDAAVRKSLLEDY
                     NIEISGGLGSLKGKIWRVGLMGYTSRKENILLLLAALKKILYK"
     gene            complement(24484..24834)
                     /locus_tag="PK898_05095"
     CDS             complement(24484..24834)
                     /locus_tag="PK898_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOU19445.1"
                     /translation="MSEETIMQGKASWQLEKTDPKEAELLKESLRNVHDPELGYSVID
                     LGLIRDVVKEENRAVITMILTTPFCPYGGHMIEQVRMAAMEALKTDVYVDLRFDPWDP
                     EMMEEGADMDWGFL"
     gene            complement(24871..26565)
                     /locus_tag="PK898_05100"
     CDS             complement(24871..26565)
                     /locus_tag="PK898_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19446.1"
                     /translation="MPDLKKSLSLHEPYILNAIAASWNLSASAESAEPIEEILARKII
                     QSIDSSSLRKDLPESVRPAIEKLVINNGKIQSGPFFRLFGEIEVMGEARFIREEPWKS
                     PQNPAQWLWYRGLIQKSFFDTPEGMREYVYLPDELYDKFRQELKNLPIEESNSPDLII
                     RPAAPREVVQIYAGNDSVLDLSCLILASLRTGHEFSLFYPVYGEKKTRFTVNLLQIAG
                     ILDQSFMPIAETGQSFLMIDRLSALLQLIRAWQQTEEMDELLWIKELAVDELFAHNPS
                     KLRTNVMQFLNRIHGNGWWSFSGFLSAVKQDSPDFLRNQNDSTAWLIRNREKNQYKEW
                     ENVEGIYLRYLLSGPLHWLGIVDIASNAPKSENPEKPNGFLPISAFRVSRIGERCLAE
                     SLGKEVPKDNYRKANLEKVPPKVTIDGRISVNPNVPRILRYHIARFCEWEKIQPDRWI
                     FRVTPESLLHAKENGLSVPSFIAMLKRSGEKALPEALLNAVEQWDRMDTQATMFQATV
                     LTVTNPDWIASLLNSKDTSQWIQQQLNANTVFIKPKGEEAVRRALIEMGVLTDTRK"
     gene            complement(26565..>27380)
                     /locus_tag="PK898_05105"
     CDS             complement(26565..>27380)
                     /locus_tag="PK898_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19447.1"
                     /translation="QNKLSLFVYIIILSIGFGSAIRRTFWAGAVPFLLHLTYSLGTAF
                     AMTSGFRFILPVDWVTLIYFAIGCVAVMRFPFIYIWEMTDQPAFTQIAITDGFPKRSL
                     SICLSFIIILLGLALPLCDSVIPRRFHSSSSSEEILNRWIEMTPENKNLAIQNQLSQK
                     IQNGQLEILEGRAVYPRIYTAHTGDSGGNSSIKVNADYDRLVWMLLNGKVSVISLPVV
                     KEDFDQPIPDPQDLIVIGEKKEDYLSAQWVTAVISSKVQTWEAASIDKKDSDE"
     assembly_gap    27381..27480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27481..29045)
                     /locus_tag="PK898_05110"
     CDS             complement(<27481..29045)
                     /locus_tag="PK898_05110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19448.1"
                     /translation="MIPILLKVDEEPHPFYNMNVSKKWFWFWLVNAFEGIASVVMIFQ
                     TASEAESAVLFGLSPLRIGLVLGLLFLIGLCLRFAKNALTEQDASDSVKDNRRYQVVG
                     LVGLLSGYIVVLAWIFLRPPFGITAFSKSLFTRLIPALVWGGLFSVESIFFVWFFSSK
                     EDIKSRLNRNRKTLIWAVTCGILLIFLIVFALKTGLGLHIISGTFYRQGVSILEGHLI
                     FPLLILFPIIGLITFLQSRFLKKKAISEKGQKNFIRLTGLIIWLVASIVWIRTPFEGR
                     SYFLPALRPPNYNFYPSSDAEYYDMFAQSILIGDGFRNGMGVVRPFYAAFLALLHAIA
                     GNNYLSVTNLQIIVLALFPVAVYRIGTLLHFPLSGILAAWWVILREVYSIELTPVVQV
                     SNSRLMMSDLPTALIVSWLMVALIHWYQADKKRQSLFAIISGGLCGIAVQIRTQSAVF
                     IPVVLLFLLIRYSIEKRLNLHTIKSFGIFAVSVLIIILPWNVWNQIHPNPTAGESNSE
                     VNYLYSLYAHAANA"
BASE COUNT         8773 a         6152 c         6203 g         7914 t
ORIGIN      
        1 aaaccaaaca gtatataata taaaaaatta tatcgggaaa ggttttatat caaatgaaaa
       61 aaaatttaga catcctaatg gtcggtcatt tcgcaaaaga taaaatcgtc atcgacggaa
      121 atgaattcga aagccccggc ggagccgtct actacggcag cgtcgtctta cgcagactcg
      181 gtttagaagt cggtgtcgca acacgaggaa acccggatga tttccactat ctgaaagaac
      241 tgaccgatct cggaatcgaa gttgacgcga aaccagcacc gcaatcctcg ggatgcatca
      301 acatctatca atccgccgac atggaacgaa ggatttgcaa actgaacgga ttcgcaggtg
      361 aaattcctta cgaagatatt ccggatgtaa atgcaaaaat cattatggtt acaccgatta
      421 tcgccggaga aattacgctc cctaccttaa tcaaactttc cgaaaaagca cctatcgcat
      481 tagatgttca gggctttgtc agagttcccg aagggaatga tctcaacttc agaccttgga
      541 aagatatgga agaaggactg aaacatgtca catttttgaa agtcgataaa gcggaagccg
      601 agcatattac cggaaccgat gatcttgaaa aagccgctgt tatcttagcc ggatatggtc
      661 cgaaagaaat tgttctgacc caatcaaaag aagtcctggt atatgcggac ggaaaatttt
      721 atcgtgcacc ttttactccg aaatctctcg ccggcagaac aggcaggggc gatacctgta
      781 ttacttctta tgtcggttca aggctaaagt atgatcccga acgatcctgt aaaattgccg
      841 gaattatcac aaccatgaaa caggaaaaac acggtccttg gaccggcgag attgatcctc
      901 aacgattcga tccttcaatc gttttcggtg attaatagtt ttttgttttt agattattat
      961 taatgtataa acaaggaggg gaaaatggca gaaaagaaag tttctagaaa aaaagtaaat
     1021 atgaatgcgg cttcttcaaa cgaagaagag aatcttgaaa gcggtactaa agaacaatcg
     1081 ctaaataccg ccgaaacaaa caattccctt cctcaagccg aagaaaatga aataaaaggc
     1141 gcttcggaaa gtcaaagcaa tattatcgaa tcaccggcca atattcatca agattttttc
     1201 gaagaaaaca aaaaagttga agaaaagaaa atggaagaaa acgaggcagc cgcaggttca
     1261 gtcaatcctg ttcccgaatt tgaaaaactc cctgatcctt tcattcaaga accggagatt
     1321 tcagacaaga agattcccgc cgaaaacgaa ggaatcgaaa aggatgcatc cgaagaagga
     1381 aaaacggttg aaattcctga aacttctcct tccggtgaac tgccgaaaga tgtgtttgag
     1441 gaaggaaccg gtgaggagaa gaagaataat aataatatct ggttatggat tgtcattgct
     1501 atcattgtta ttattttctt atgtttttgc ggttttatcg gctccatcgt ctttctgttt
     1561 tggggctaac cggcgtttat cgaatactat tgttccatat cgattgagac aggatctttt
     1621 atagaacaaa aagatcctgt ttgtttgcta taattatgct cctatgaaag aaaacaatcc
     1681 gatcgtctat tctaccgaaa aaggacgcat ctgtcctgaa tgcgattttc cggaaaaaga
     1741 atgccactgc cggacaaaaa agatctcgaa aattatttcc gatgatggca cgattagaat
     1801 tcggaaagaa aagaagggaa gaaacggaaa agaagtcacc ataattcaag ggatctctct
     1861 ttccgaacaa gccgttcaat cgattgtttc cgatatcaaa aagaaattgg gaaccggagg
     1921 aacggttaaa gacgacatca ttgaaatcca aggggatcgg gtgaagttta tagcggaata
     1981 tttcattaag ctcgggtata aagtccaaat aaatgggggg taagatggcg gttgaacaaa
     2041 acaaaatcat ctattcgatg atcggggttt caaaatatta tgataaaaag gttattctca
     2101 aagatattta cctctcctat ttttacggtg caaagatagg tgtcctcgga ttaaacggtg
     2161 ccggaaaaag ttcgttatta aaaattcttg ccggagttga taaagatttc atcggtgaaa
     2221 ccgttgtagc acccggttat accatcggtt atcttgaaca ggaaccgttg gttgacagta
     2281 aaaaaacagt tcgtgaagtt gttgaagaag gtgctcaaga agctatcaac ctattaaaag
     2341 aattcgaaga gatcagtaac aaattttccg agcccatgag tcccgatgaa atggatcgat
     2401 taatagaaaa acaaggcaaa attcaagaga aaattgatgc actggatgct tgggatatcg
     2461 attcccgctt ggaaatggca atggatgcct tgcgttgtcc gcccggggat tctccggtcg
     2521 acattttatc cggaggggaa aaaagaaggg tcgccttatg tcgtttattg ctccaaaaac
     2581 ccgatatcct tcttctggat gaacccacca accacctcga tgccgaagct gtggcttggt
     2641 tagaaaggca cttacaacaa tacaaaggaa cggttatcgc cgtcacgcac gatcgttatt
     2701 ttttggatca tgtcgccggc tggattcttg agttagaccg cgggcaggga attccttatc
     2761 agggaaatta ttcatcttgg ctggaacaga aaaaaattcg tttggaacag gaagaaaaag
     2821 gagaaagtca aaggcaaaga acgcttgctc gggaactgga atggatccat atgtctccca
     2881 aaggacagca cgccaaatcc aaagcgagaa tttcttccta tgaaaagctt ttaacccagg
     2941 attttacaaa agccaatgag gaattacagc tgtatattcc tccgggtccg cgtttgggga
     3001 atttggtggt tgaaatgaaa gatgtccaaa aagtcttcgg aaaccgaatt attatggatc
     3061 acgtgacctt tacgctccca cctggcggag tcatcggaat tatcggaccg aacggtgccg
     3121 gtaaaaccac tttatttcgc atgatcgtcg gagaagaaaa accggattcc ggtaccattc
     3181 gaatcggtga gactgttaaa ttgggatata tgaatcagag tcgggactct ttggatccgg
     3241 aaaagacaat atgggaagtt atttcggaag ggcaggatga aataaagttc ggcgatcgaa
     3301 aaatgaattc cagagcttat gtcgcccgtt ttaatttttc cggagccgat caacaaaaaa
     3361 aagtcggagt cctgtccgga ggggaaagaa atcgcgttca acttgcatta actttaaaaa
     3421 gcgaggcaaa tgttcttctc ttggacgagc cgacaaatga cctggatgtc aatacgattc
     3481 gtgcacttga agaagggctt gaaaatttcg gaggttgtgc tcttgtcatc tctcatgatc
     3541 gttggtttct cgatcgcatc gcaactcata ttctcgcttt cgaagatgat tcttctgtca
     3601 tcttttttga cggtaactat acacaatatg aagcggatcg acgaaatcgt ctcggcattg
     3661 aagccgatac gcctcaccgg atcaaatatc gatctttgac ccgaatataa gaaaaaatca
     3721 gcaaccgtta ttgatatctt gctgatcgat cagaaggaaa ataactcttt ccgctgttgt
     3781 tgatgtcgat tcaattcatt tactgacaat atgaattatt tcatttaaat caattacatt
     3841 tctcgtaatt cgattcatta tttccatgtt atgataaaag aaggttttgt aaacgtttac
     3901 aatgccaact gacagataac agatagataa actttttatt ttcactatcc ctaaggagtt
     3961 tgttcctatg aatagaattc gagtcggaat tattggagca ggccggatcg gacgagtcca
     4021 tgctcaatca attgcatttg gaatccccga ggcagaaatc gtcgcgattt cagatccctt
     4081 tattgaaaat gcggaaagag ccgcaagaga tttcgctatt cccgccatat atgcggatta
     4141 tcatgccatt ttgaatgatc ctactattca agcggtttta atttgttctc cgaccaatac
     4201 ccatgcggac attgcaatgg aagcagcccg agccggtaaa cacatttttt gtgaaaaacc
     4261 ggtagatcaa actgtttcaa aaatacttga aacaaaaaaa ttagtcgaag agaacgccgt
     4321 aaaaatgcaa atcggcttta atcgccgctt cgatcataat ttcagaaaga ttaaaaaact
     4381 cagagatgac ggaaccttgg gagaaattca aattatccga ataacttcca gagatcccga
     4441 accgccttca ccggaatatg ccaaaacttc cggcggaatg ttcttggata tgaccattca
     4501 tgattttgat atggcatgtt accaagcaaa ttctgaagtt gatgaggttt atgcaatggg
     4561 tgctgttacg gtagatcctg aaatcggaaa agcgggcgat atcgatacgg caatcgtgac
     4621 actcaagttt aaaaacggcg ccatggctgt tattgacaat tgcagaaaag ctgtttatgg
     4681 ttatgatcaa cgggtcgaag ttttcggaac aaacggagcc gcggcatccg aaaacgatcg
     4741 ggaaacaatg gttaaaatta gttcaaaaga tgcggtttgc gaagataagc ccttattctt
     4801 ctttttggaa agatatatgc aatctttcag agatgaaata aaatctttta tcaatgcgat
     4861 tttgaacgat cgacccgttg aagttaatat tgatgacgga ttacgtccga tcctgatcgc
     4921 aattgccgca aataaatcga tgaaagaaaa tcgacctgta aaaatatcgg aggttatgga
     4981 aggataatat tatgagtaaa aagattatca aaggaaaacc attcgatttc ggctataatc
     5041 cgattacaga aatcggtggc gatccggata tgttaatgga ttttggcatt ttaaggctaa
     5101 aatccggaca gatttatgaa gacaactctg ctttggaaag agcttatttg ttggtttacg
     5161 gagaaataac actcgaatat aataaatcat ccataaaaat taatcgaccg aattgttttg
     5221 attatgctcc ttgggtattg cacttgccgg ctgatgttcc ggtaaggata acgggagtgg
     5281 cggaagattc agaaatcact gtcaataaaa ccgttaattc gcgaattttt gatccaaagc
     5341 tatacgaacc taaggattct ccggatgaat tcagaggagc cggaacgatg caggaaacat
     5401 ccacgcgcat tgttcgaact acatttgatt acaacaacgc cccctatgcg aatttggtaa
     5461 tcggagaagt gatcggtttc cccggcaaat ggagttccta tccgcctcat tttcatccgc
     5521 agccggaaat ttattattac aaaacctatc cgaccggcgg ccatgcattc gccgaagtcg
     5581 gtgatgatgt tttcaaaata agcggaaatg atacagtcct gattcaaaac ggaatgactc
     5641 atccgcatgt tacttacccc ggatatgccc tttggtattt atgggcgata cgtcatttgg
     5701 acggaaatcc gtatattaca ccaacctttg taccggaaca tgaatgggtt gccaaaccgg
     5761 atgcaaaatt ttggtcttac ggcaataaaa aggagatgaa atgatcaaga gaatccgact
     5821 gacaatggct caggcgctta ttaaatttct ggataatcaa tatgtcgaat ttgacggtga
     5881 ggaagaccgg ttcgttgaag gaatgatcgg agtctttggt cacggttgcg tcaccggcgt
     5941 cgggcaggca ttggaacaag gcggacacag tctgcgattt tatcaggggc ataacgaaca
     6001 gggtatggca catgttgcaa tcggatatgc caaacagaaa aacagacgga aaatccttcc
     6061 ctgcctgtcg tccatcggcc ccggcgcttt gaatatggtt accgcatgcg gtaccgcgac
     6121 agcaaatcga atccctttac tcgttttacc gggagatact ttcgcttgcc ggcagccgga
     6181 tccggttctg caacaagtcg aatttccgga tagttatagt acgacagtga acgattcttt
     6241 ccgagcggtt tgtcgctatt gggatcgaat cagtcgaccc gaacaattaa tgacggctat
     6301 gatccatgcg atgagagttc tcacagaccc cgcggatacc ggtgctgttt gcgtggcttt
     6361 gccgcaagat gtcgaagccg aagcttacga ttatccggag tattttttcc gtaaacgagt
     6421 ttggcatatg gatcgccgtc ccataacaga tcgacaattg gaacaggcaa tcgacttgat
     6481 ttcaaacagc aaaaagcctc tgctaatttg cggtggaggt gtgcgctata gtgaagcgtg
     6541 gaaggattta caaaaatttg ccgaagaatt taatatccct atcggtgaaa ctcaggcagg
     6601 aaaaggtttg attgcttggg accaccctct gaatctgggc gggatcggag ttacaggcac
     6661 caaagccgct aatttaattg cgagagacgc cgacttgatt atcggtgtcg gaacacgttt
     6721 caccgacttt acgaccaatt caaaatgggg attcagaaat cccgaatgca agattctggc
     6781 gattaatgtg tgctcttttg atgccgtcaa gatggacgcg gaagggatat tggcggatgc
     6841 ccgtgaagct ctctcggcga ttcgaaaaga gttaaagaat ctcaattacc aaagtaaata
     6901 tacggacgaa attatcgaag ccaagaaaga atgggatgat atcgtcaatg aatattacaa
     6961 ccttccggaa gaaaaaaacg gtattccgca aaccagagca ttgggagaaa tcaatcgctt
     7021 catgcgctca aaagatatcg ctgtaggatc tgccggaagt ttgcccggag atatgcagag
     7081 gctgttccgc ccgggaaaac ccggtactta tcacatggaa tatggttttt cctgcatggg
     7141 atatgaaata aacgcagccg tcggggtaaa aatggcggct ccggatagcg aagtgtatgc
     7201 tttcttgggt gacggcagtt ttattatgct tcacagcgaa ctttttatgg caatccaaga
     7261 aggattgaaa atcaacataa tggtttttga taactcaggt tggggctgca tcgagagtct
     7321 ccaaaataat cagggaacaa aaacatttgg gactgtcttc cgggcacgta atcctgagac
     7381 agatttgttg gacggagaaa ttcttccggt taacttcgca aagatcgctg aaggatacgg
     7441 ggccaaagga tatacgattc ataatgcgga tgagctgaaa aaagcattga agaattctaa
     7501 aatgcaagaa aagccctgtg ttttcgatat taaagttctt cccggcagca tgactccggg
     7561 atttgaaagt tggtggagag tgggtgtggc ggaagtagcc gaggatcaat ctgtaaacga
     7621 agcgtacgcg gaaatgcaga aacatattgc cgaagctcga gattattagg aaggggagaa
     7681 atgcttaatc cggagaaagt taaacttgga attgctccga tcggatggac aaacgatgat
     7741 atgcccgatt tgggagcgga aaatactttt gagcagtgta tcagtgaaat ggcgttggcc
     7801 gggttcaaag gctgtgaaat agggaataaa tttccctctg atcccgctgt cttgaaacac
     7861 aaattggaag tccgtggttt acaaatctgt aacgcatggt tttcaagcta tcttatcggc
     7921 agaccctacg aagaaacgat cgcttctttt atccgccatc gtgacaaact gaattatttg
     7981 ggagcgaaga tcatcggtgc atcggaacag ggaaattcga tccaagggaa aattgatgtc
     8041 cctatacttg agaataaacc gatttatacc gaagaagaat ggaaaatcgt cacaaaaggg
     8101 tttaacgaaa tgggagaact tgcccgcgaa aaggggatgt ttttcaccgt acatcaccat
     8161 atgggaaccg gcattcaaac agaagaagag atcgatcgaa tcatggaaat gacggatccg
     8221 aatttggtat atcttttatt cgactccgga cacctgagtt ttgcgggtat cgaccctgtt
     8281 catatattga aaaaatatat tcatcgtgtg aaacatgtcc atctgaaaga tctgcgatac
     8341 aacgtctatg ccgaagtcaa agcaaaaaga atgagttttt tggacgccgt tcgagccgga
     8401 gtatttactg ttccggggga cggtgacgtt gattttaaac cgatctttga tattcttgaa
     8461 gaagcggggt atgaaggttg gatcgtagtc gaagcggaac aggacccggc aaaagcaaat
     8521 ccatttgaat atgccgtcaa agcacgaaaa tatatccggg atattaccgg tttgtaatac
     8581 ttttgattgg agggaaaaat ggttaaagtt ggagtatgcg gttatggtgt aatcggtcaa
     8641 cgattggctg atggggttgc gttacaaaaa gatatggaac ttgtcggagt tgccgacgtt
     8701 gcgcctacgt tggcaattcg tgctttaaaa gaaaaaggga tgccttatgc tttgtatttg
     8761 gcatccgaag aaaaccgagc aaagtttgag caattaaaga ttcccgtttc tggaacattg
     8821 gaagaactgg tccggaaatg cgacattatc ttagacgcca ccagcgccgg tgtcggggcg
     8881 aaaaataaag aattatatgc aaaatataat aagaaagccg ttttccaagg cggcgaaaag
     8941 aattcggttg ccgatgtatt cttccacgga tatgcgaact atgaaaaagg aatcggtaaa
     9001 caatttttga aactcacatc ctgtaacaca accggattga tccgggcggt tgactgtctc
     9061 gacagagatt atggcattaa aaaagtggcc ataacgatcg ttcgcagagt cgccgatccg
     9121 ggcgattatc atcgcggctt aaccaacgct ttacaaatcg acaaagcacc aagccatcag
     9181 gcggtcgact tgatgacgat catgcctcat atcgacgcca ccggaattct gattcatacg
     9241 cctgtgacgc atggccatat tattacggtt gttgccaccg gtcataaaaa gatcaccaaa
     9301 gaaatggcgc tcgaatcgtt tctgaaacac gatcgaattc gggttgtcag taccgacgac
     9361 ggatttttag gaaatgcttc cttcttccgt tacgcccgtg atttaggaaa cccgcgcggc
     9421 gatatgtatg aaatcgggtt atggtcagac agtattgttg aatcgggcga cgagattatg
     9481 tttgcaatca atatcccgca ggaatctgtc acgattccgg aaacgatgga tgctgtccga
     9541 gctgcctgcg aaatgcaaac agatcgattg gaaggtatcg gtaagacaaa tacgtatctc
     9601 ggaatgggaa aatggaaata aaaatgctct cactcgaagg agtagatctt cgaggcaaaa
     9661 aagttctttt acgaccggac atcaattcac cgctcgatcc gcaatccaag gaaattgtga
     9721 atgatgaacg gatcgtaaaa acgataccgg taattcaaat gctgttaaaa ggcggtgctt
     9781 ctgttgccat catcgcccat caaggtgata ccctggatta tcagaattta accgatctat
     9841 cgaagcatgc tcagaaaata tccgagtatc ttgatcatcc ggtggaatat ctggatgacg
     9901 tctgcggtcc ggcagctgtc caaagagtca aggcactgaa accgggtgaa atcgtaatcc
     9961 tcggaaatct tcgttatctg acggaagagg tttctacttt cgaaagagac gttaaattgg
    10021 acgccgccgc atataccaaa acctgggaag tccgtaacct tgctccgttg tttgatttgt
    10081 atgtgaatga agctttttcc gcagcccatc ggaattcacc cagtatggtt gcattccagg
    10141 aattacttcc gacctatgcc ggtcctcttc tttttcagga atacgaagca ctgtcgaaaa
    10201 ttcttcatgg tgctcaaaaa cccgctgttt ttgtattggg cggtgcaaaa atctccgatg
    10261 cgttcggaat gatgaagcct gtgcttgaaa acgggaccgc tgataaaatc ctgacttccg
    10321 gagtgaccgg cttggtaatg ttgatagccg cgggaataaa tgtcggagag aaaactgtcc
    10381 gatttctgaa ggataaagat cttctgggat tcgtcgaaga ttcaaaagaa tacctcaaga
    10441 attatccgga taagattatg atgcctgttg atgtggctat cgaagaaaac gggaaacgcg
    10501 ttgagatcga tatcgacgaa atgccgaaag atacgcttta tcttgatatc ggcgcaaaga
    10561 cggtcgctgc ctatcgggaa gttttgatgc acgcaaaaac tatttttgca aacggtccgg
    10621 ctggcgttta tgaaaatccg ttatttgaag ccggaaccaa aggattgtgg caggctattg
    10681 cggactctcc ggcttattcc gtcatcggcg gcggagatac aatcagttcg gcaacccgcc
    10741 tgatcgatat ctcgaagatc ggctatgtgt gtacaggggg tggcgccatg gtgcgattta
    10801 tgtccgggaa aaaattacct ttgatctctg cgatggaaaa ggcttatgaa agagatcaat
    10861 cgagaacaat ctaagcgatg aaataaacgg ttctttgtct ttatcaatat ttgagaaaaa
    10921 gataacccga gaatgtgcgg atttctcggg aaatatcata atcggtcaga cttataagat
    10981 acaattagca ggaagttgca gaatttttga tcttgcacaa acagaacgaa agcggattga
    11041 aaggaaacaa tattatatga ttactccctt aaaagaattt gcaacagaca tcagaataca
    11101 ggaaatgatt gaattcaaag cccgcggttt cggccatacg ggtggttcac tttccgttgc
    11161 cgattgtctc gctgctctct acggtgggat tatgcgaatt gatccgaaga acccccaatg
    11221 ggaagaccgt gatcgattgg tcctgtccaa aggtcatgca gggccggctc tttatgcgac
    11281 attggcacta aaaggctatt tcccgttgga agaattaaag actcttaacc aaaacgggac
    11341 aaaacttccg agccactgtg atcgtaattt aacggtcggt gtcgatatga ccaccggttc
    11401 tttggggcaa ggtgcttcga cagcatgcgg aatggcactc ggcttaaaac actcgggaaa
    11461 agatgctcgt gtatatttaa tcctcggcga cggtgagtgt caagaggggc aggtgtggga
    11521 aatggctctt tttgcggccc accataaact caataacctg atcgctttca tcgattataa
    11581 caagcgccaa ttggacggtt atgtaaagga tatctgcgat ttgggcgatc ttgtggcaaa
    11641 attcaagagt tttaattggt atacaacaat gatcaaagga gatgacccgg atttgatccg
    11701 acaaactgtt cttgatgttc aaaagaatca gggagatcgc ccgggaatga ttgttctcga
    11761 tacgattaaa ggagcaggtg tccctcaaat cgaaaatatc ccattaaatc atcatacgaa
    11821 tgtcactccc gaagaagcgg atgaaataat tgcccagctt gaagaattca agaggaaagg
    11881 ataagctgtc atgtttaaac taatttacaa tggtgaaaaa gagaaaaaag ccgggaaaaa
    11941 agttttcggc gaaacgatgc agaaaatcgt tgaacaggat ccgttggcgg tttatcttga
    12001 tggcgatatc atgaattcaa tcgggacttt aggtctggaa aagaaattcc cggatcaagt
    12061 tttcgaatgc ggaattcaag aagccgatat gatggggatt gcgggtgggc tttccgctgt
    12121 aggaaagaaa ccttatgtgc atacctttgc ggcttttgcc gcacgtcgag cattcgatca
    12181 agtctttatt tcaatcgcat acgcaaaaaa taacgtaaga ataatcggtt cggatcccgg
    12241 cgttacctca gcttataacg ggggaaccca tatgcctttt gaggatattt gcctttatcg
    12301 cgcgattccg cattcaacgg ttcttgaatt caccgacagc gctatgcttg catctcttct
    12361 tccgacatta aaagacagac ccggtttaac ttactgccgc ttaaaccgga aagaaggaac
    12421 cgctatttat tccgaagatt ccacttttga gcccggaaaa gcaaatttgt tacgggacgg
    12481 aaaagatgtg acgatcgtgg cttgcggtat gatggttggt gaatcgttga aagcagccga
    12541 aatactggaa aaagaaggga tttcagccgc tgtaattgat atgtttacca tcaaaccgct
    12601 ggatgaagag attatcaaag aatttgccgg taagactcat gccttcgtta ccgctgaaaa
    12661 tgcaaatatc gtcggtggtt tgggagctgc ggttgccgcc ttcctcgctg aaaccactcc
    12721 ttgccccgtt atccgggtcg gagtaaaaga tgagttcggg caggtcggaa cgccggaata
    12781 tctggctgaa gtctatggtt taacttcaaa agaaattatc gaagcagtga aaaaagctgt
    12841 ttccatgaaa taaaaggaag aaagagggtc accgtatttg catggattgc ggtgatcctt
    12901 ttttaatatc tatggctgat gtaacgatca aagatatcgc ggaggctaca ggagtatcct
    12961 acgctacggt ttccaggaca ttgaactccc gcaacggagt cagtccgaaa actcgcgaaa
    13021 aagtcctgca aaccgcaaag gaaatgggat ataaacccaa tatccatgcg cgcagtctga
    13081 aaacaaatca aacacttacc atagcattaa ttgttccgga tatatcgaat ccgttttttg
    13141 cggatatcgc gctttcggtg aataagaccg cttttgctaa agggtacaac acaattttat
    13201 gcagcacaaa ttgggatccg gaggtcgaga ggctccaatt aatgcatatc caagaacagc
    13261 gtgtggatgg cattattttt aaaccgtgtg atcgttgtac ggatcgatat atggatattc
    13321 atattccgaa agtaatgatc gcgaatttgc tgaaaacgga aggattgagt tatatcgaag
    13381 ttgataatat tttaggaggg cagaaagccg cgtcgcattt aattgcctgc ggttatcgaa
    13441 aacccgcttt cttgggtggg gagatcgttt cgaattcaaa tgccgatcgc ttattcggtt
    13501 tccaatcgac ttttcagacg tttggcattg aaattccgga tgaacggatc catttcggcc
    13561 cttttacaat tgaaaacggc tatcaatccg ccaaacgaca gatagaatta tcatcgtctc
    13621 ccactccgga ttgtttcttc tgcgggaacg atctgatcgc tctcggtgcc atgcaatatc
    13681 ttgccgaaca acgaatcaga atccccgaag attttggtgt ggtcggtttt gatgacgttt
    13741 actttgcatc attaccgcaa atccaaatga ccaccattgc tcaaccgaga aatcaaatgg
    13801 gggttcttgc cgcagaaata ttaattcgtc atatcgaatc gggggataac gaggaagagc
    13861 atattttact tcaacctgaa cttatcattc gaaacagcac cagaaaaccg ctccgatagt
    13921 aaaatctatc acaagaggaa cttatgaata tttcatttgg cttcggacag tcaaccgttc
    13981 aattaaatat accggacaag aatcttctta aggttcttga ggcaaatccc attcaaatga
    14041 tcggagatga atcggaaatt gtgcaaaagg cattacaaaa tccgattcaa tctcgacgtc
    14101 tgaatgaaat tgttcggaaa ggtgaaaaaa tagcaattat cacaagcgat atcacccgcc
    14161 cgatgccgtc tgctcgtgta ctgccatttg tattagacga gttgtcatct gcaggaatac
    14221 ccccgtctga tatcgtcatt atttttgcgc ttgggaatca tcgttctcat acggaagaag
    14281 agaaaaaaat gctcgtcggg gaagagatat tcaaaaaata cgaatgtatc gattctgacg
    14341 gaccgatggt tcgtttggga atcaccgcga atggtacccc ggtagatatt tttgaacgcg
    14401 tcgtcaaggt cgacaaacga atatgcctcg gaaacatcga ataccattat tttgcgggct
    14461 atagcggcgg agcaaaagcg atcatgcccg gtgtatcgac ccgtgaagcg atacaggcaa
    14521 atcacagaca tatgacagat ccgaatgcca ttgccggaaa aattgaaaat aatcctgttc
    14581 ggaccgatat cgaatcggtt ttaaatttta tttccgtcga ttttattgta aatgtaatac
    14641 tggatgaaca taagaaaatt gtccgttgtg ttgccggaga cgttattgcc gctcatcggc
    14701 agggatgccg atatttggat caaatgtata gcgttgaaat ccccgagaaa gcggatatcg
    14761 tcctcgtttc tccgggcgga tatccgaaag atatcaacct ttatcaagct caaaaagcgt
    14821 tggataatgc aaaaaacgct gttcgaaacg gcggatcaat tttgtggatc gcttccgcta
    14881 aagaaggttt gggagaaaaa acttttgaag aatggatgtt acaccataat aatccttctg
    14941 aaatgatcga tcacattcaa aaagaatttg ttttgggagg tcataaagcc gccgcaattg
    15001 cgatggtcat gcaaaaggcg accgttattc tttattccga actgaatccc gatctagtgc
    15061 gaaaaataaa attggaatcc actcaaaatc cacaggaaac cctcgaacga cttataaatc
    15121 aatatggaca aaatgcatcg attatcgcga tgccttacgg cggtgccaca cttcccattt
    15181 tagctgataa aaggtgaatt atgaaatatt acggagcaat cgaagcaggc ggtacaaaat
    15241 ttgtttgttc gatcttggct gatcccgatc attattttga agaagttcga tttcctacga
    15301 caactcccga agagacgctt gaacgcacgg tttctttttt caaggagcaa cgaaataaat
    15361 atgagctcac cgctatcgga gtaagctgtt tcggacccat tgatttgaat ccctcttcgc
    15421 ctacttacgg ctatatcaca accacgccta agataaaatg gagatataca aacatcgtcg
    15481 gatttctgaa acaagaatta aatttaccgg tcgggtttga taccgatgta aacggcgcag
    15541 cactcgccga atacaaatgg ggcaatccgg aaaacatccg caacctcgtt tattacacag
    15601 tcggaaccgg agtgggtgga ggcgttgtta ttgacggaaa gccggttcat ggtttggtcc
    15661 accccgaagc cggacatatt ctgatccgtc aaaatcttga aaaagatcct tttaagggtg
    15721 attgccctta tcatggaaat tgtttggaag gtctatgtgc gggaccgaca attcaagccc
    15781 gctttgggat tcccccgcaa aacctgcccg aagatcatcc tttttggccg attttggcta
    15841 attatctggc tcaagcctgt atcgcccaaa tttatcagat ttccccgcaa aaaattgtcc
    15901 tgggcggagg tgttatgggg cagaaacaac tttacccgat gattcgtaaa gaggtcattc
    15961 gtttactgaa cggttatata caacatccga tgattctcga acatattgat cagtacatta
    16021 ctccgcctat tttaggaaat cgtgccggtg cttgcggtgc gatggcatta gcacaaatgt
    16081 tggaaaaaca ataattttta cttacaaata ataaccggaa tggaagtcac cgaaaggact
    16141 ttttcgatgg tacttcccgt aaaaagtctt ttgatatagg agcgggaata tccgcccatg
    16201 ataatcaaat cggatttttt tcgattcgca ttctcaataa tcgctttgcc gggttcgtcc
    16261 gaacttgtga catattccgc ctgaatgcct tgcgcttgat aataattttc tacttcctgc
    16321 cgggtaactg cccgtctctg ctcatcaccg ccggaaacga caaccgttat atgcccgccc
    16381 gtgctttgcg ctaagccgga tgcgaaatac atggcttcac gagatttcgg gctctgtgaa
    16441 aaagcaagta aaatctgttg aatcttcgaa ggaaacagat cgggagtaac aaatatcggt
    16501 cccggaacac gcataaaaat agaattccaa cccgatccca acgaccggta tcgtgaaggc
    16561 gaaggtctgt aattcatttt aaataaggaa atatcactcc aaaaagcccg atctgaaagg
    16621 ttcttcagta aatttccctt aagcacaaag aattttccct tgacattttc tttttccaaa
    16681 agagtttgaa tatcctgttc atatttccgg atccactcgg aatcgagcat ggaaggtctt
    16741 tttacaacat gcacaattcc cgcccaggcg ttgaattgct tacaaaaaca gactgcactc
    16801 tgcagataat caaaatcttc aatcggatcc cgcacggata tcatcatacg ttgtatggat
    16861 aacgaagaat catcctttcg caattccttc cgccagtctc cggtcttaat tccccaatcg
    16921 gaaagatccg gtgaaaaaat cataaagaaa gctttcttaa aacgatagcc gatgcttttt
    16981 ttctgctttc cgtattcttc gtccaatttc cacgccaaag cggtcattcg taatccttcg
    17041 ccgtactctt cggttaaagc agctttatta ttttcgatcc agaggtagag ctcggtttcc
    17101 gttttcccgg ggaattcatc cataattttc tgagaacgaa taacttcaat aaccggcata
    17161 tagactgtgc gataccaatc catcacagct tcatcctttg aaatatctct tttataatcc
    17221 aatcccataa aatatcgatg aacagcgata tgttccgcca actcggggta ttcaccgggc
    17281 aggtacattt ccaggttggc ttcaggaata agtttttcga tattggtctg atcaagaaaa
    17341 gcttgttttt ctgtttttac aattatttca tcgggtgaat cggtcggttt taagggaact
    17401 tcggtgttga tgatccgaac attcgcatta atatattgcg ccccatagct tttcatcacc
    17461 gaaatacgat gatgtccatc caaaacaaaa taaacttcac cgatttgata tacatcaatg
    17521 ggcggaacgc cttccatcga ttcattcgcc aaccgaacgt tcacccaacg gtttttatcg
    17581 taatccatct tgggcaaaaa cgtccttgtg aaatcattat ttcgagatac ggatcccaca
    17641 atcgcatcaa tcgggatatc tttttgtaca ggtaacgcgt tgactgtctt ggctaagcgt
    17701 tttctgactt catcatagtc gattaaatta taatttttcc gttgcagaaa accgagtgct
    17761 tgttgccaaa tcgcttggtt tctggcccga tcaaattctt tttgtgcgtc tcgaattccg
    17821 tacattcatt tacccggtta ataagttcat tgtttcaact gtattttact ttgctttatt
    17881 tggggaactc attgcaaatc gttcaaaaag gacaacaatg gagtaaagtg gaatgaattt
    17941 atgaatcaaa tcaaaatcta ctgtcggttt gagagcaagt cgtcaactca aacccatatt
    18001 tcattaatag gttttcattc gtcaaaattt ctttaatgga accttcggca accacttctc
    18061 ccttggataa aataatggcg cgatcgcaca gttcccaagc caaatcgata tcatgcgtag
    18121 cgatcatttt ggtatcacga atttctttta acagattgat taccgttctt cttgaacgtg
    18181 gatcaagaaa agaggagggt tcatcaaata gaatcgcctg cggttccatt gccaaaacgg
    18241 tagccaaggc agccaacctt ttttcgccac cggataattt atagggagcc ttttttaaca
    18301 aatgaacaat tcccaatctt tcggctatat ctcggatttt tttattgatt tcttcttcgc
    18361 tttttccgta atttcttaaa ccgaaagcga tatcctcttc aacagtcggc ataaataatt
    18421 gatcatccgg attttgaaat accaagccga tctgagaacg tatatctttt agggtcggag
    18481 atgataattc aatatcattc acaagaatct ttccttcaga aatttttaaa agccctaaca
    18541 ttgtataaaa tagtgttgtt ttccccgcac cgttttctcc gaggatcgca atcgaatctc
    18601 catcgtgaac tttcaaatta atcgctcgga gagcgaatgt cccgtcttca tattcaaccg
    18661 ataaattttc tatggtaacc attaattcct tttattaaag aaagagtcga cttaaaacac
    18721 cggaaatatc ccatactctc agaccaaaaa tcaaaccgat cattcccatt ccgattaaaa
    18781 tgtcaaccgg tttgagagga tttgatgaac ttatatttga aatattgcca tatccgcgac
    18841 atttcatcgc aaaagccact cgtgcggctc gatcataact tcgcagaagg atttgtccga
    18901 taaaactgcc gatgtgtttc aactcgattc tctgcttccc gcaagatcgt aacaaataag
    18961 cggtttccat gacagatact tcgtccacca ccaaagttat ataacgatag ctcaacatga
    19021 tttgggtgat aatcaattcc ggtattttga tgcttctcaa caaatcggta agcgacttca
    19081 tgggtgtcgt ggtcaataaa atcaatacgg cactcacggt caaaaaagtt ttcagaatta
    19141 gggtaataaa agagactgcg ccatatgtaa tcggcagcga atccataaaa aaagcaattc
    19201 ttcgatcaaa ccaaaggtta gaaagcccgg caaaaattac aaaaggaaat actggcaaaa
    19261 tccgctgaaa aataaatcga aagggtattt ccgccaaaga gatcgccact gcggggaaaa
    19321 gaataaaagg agccagacga tcaaattgat aaggtgatac ggaaagaaca gtgattaaat
    19381 aaaaaaccaa aacaacactt tttacatagg gatgtaattt atgaataacc gtgtttccgt
    19441 ttgccaactg ttcgatacga acaatttcac tttttttctc attaaaacga ttaataagtt
    19501 ctcctcaatc ttgatccaaa gagatttaaa aaaatctatc cgatattatt cgatattact
    19561 ttctcttttt cgctttttca gctttgaaat gagccaaccg cttacgccgg cgatgacaaa
    19621 taccaacccg cttcccaaaa atccggctac ggtcgttccg tttatattcc ccaaagtttt
    19681 accttccttg aaattgtaat caggtaaaac ggaatgttga tgttgaattt ccgcactttt
    19741 attaggaagt gaatcttctt cgttgaattc cgtagaatct gttatttgag cgattgacca
    19801 ctccaatcca tcggggttag ttgaggcatg aagagagaaa aagccggcgg tcaaaatcgt
    19861 tacaatcgaa aagctggcta aaatgaggcg cagcggtact ttattcggct gctcctcctt
    19921 tttcagaaaa tgtaaaatct cgggacgaac tttaaagaca taaattaaaa cggaagcggt
    19981 aatgacaccc tcgacaagac cgatgggaag atgaatgggt aacatcaaca acaaaaattc
    20041 tctgaacggc aaagctgtaa ttccggataa caatgtctca accacgacgc aaaacgcacc
    20101 cgcttccaaa ttgactgttg aagaaatgac cgaagcaaga aagaaattcc tcgttgtatg
    20161 tcctcttttt atgatcggtt gaaatatcaa agggtatatc aaaaagcacg gaataacacc
    20221 catattaaaa atattacatc ctaaagcgat taaaccaccg tcggcaaaaa ataaagcctg
    20281 aatcaacaga actacagaaa gagtcaggaa tgcggggaaa ggtcccaaaa cagccgctaa
    20341 aagaattcct cccccgatat gcccgcttga tccggtaccc ggaatagcaa agttaaccat
    20401 ttgtgcagca aagacaaagg ctcccatcac acccataagc ggggctgttt tttcttccga
    20461 atccgattct ttgatctttt taaccgaata agcgattgct ccggcactgc cggcccacat
    20521 cattccaccg acagccggat taatcaaagc atccgtcata tgcatcgcaa ttcctccgaa
    20581 ttaaataaaa ataccatttg cggtttcaca gtgcttcaag cctgtgttct tccttcatgg
    20641 cattttaaga aaatcaaaac atgatgatgt cttcatgaca tcgttggact tatcttatcg
    20701 aatacttaac gttttgtcaa tttatttttc atttttataa ttcaataccg tttgcacagc
    20761 ttgattaatc gcttcgccga gtgtcaaatc atgcatcccg gcgacggtaa tcttgcattt
    20821 attactggga attaatattt tttcggcttt acttttccca accgcaacgg caatcgcggg
    20881 cgtgatctct cccaataagg aattcgccgc gataatcccc atcggaccga tcaaaatatc
    20941 cgcatcttca cacgcgacca aaatagggtt ttcaccggtt gcggcaaaat ccgcaccggc
    21001 ttttaacatt gtcgacgtcg caatactatt cgttccgatt gcgataatcg gatccgaaaa
    21061 tgaattcttt tttaattgtt cgataattgt tttcccgata ctgccgccct gcccgtcaat
    21121 caccacaatt ttcatcgatc atttcctttc cgtctattat tacggaccat ttgaacaaaa
    21181 agcaggcttg tcttcttgaa tacaggcctg cttttattcg tcaactttta taaaattact
    21241 ttcttgactt taagaactcg tccgctgcat ttccgaacac atgatgcaag agtaagtcct
    21301 ttttgctctt gatatacggc aaattgactt catcttccaa aaccggcatc agataagaag
    21361 cggggcgata tttcttaact tcaacatcat ctcccaaaat cttctttctg aattcctcgt
    21421 caatcggacc cggcgttttt ccgaattcgc ctcttaagat cattttgaac tcattggaaa
    21481 caaatgcata cctctctcca gcgagaacat tgaatgcggc ttgagatccg acaatctggc
    21541 tggtgggcgt aaccaaagga acataaccga catcctttcg aactttcgga aactcttcca
    21601 taacagcgtc catcatgtct cccgtaacac ccatttcttt catttggctc tgcaaatttg
    21661 aaatcatacc gcccggtatc tgggtaataa aaactttatc atcataacct ggataatcca
    21721 aatccgccaa aatttctctg cttaccgcaa tagcggtatc caaatcttcc ggagtcttca
    21781 ttatggcttc aaccatttta tcgaccttat ccaccggaac atcatcaaag taaaccggtt
    21841 ttcttctcga tttgccaaaa gaagggatag atcctttcaa ttcttcaaaa ataactcgca
    21901 atacatcttg agcctttgaa atcgcatctt tatccaagtc atgttcaaaa cccattcgtt
    21961 cggcaacaac cgccatcact tcaatcggcg aatgggaggg accacccgca aacggggaaa
    22021 tacaggtatc aaattgatca agtccgtatt tcattcccaa agcaaggtta accagcgcaa
    22081 caccggtggt tgtatgtgta tgcagaacaa tcggaacatt aatttctttc cttaaagccg
    22141 ggaacaacac gacggcatcc ttcggatgga gtaaaccggc catatcttta atggcaattc
    22201 gatcgacgcc ttcttccaag agacgctttc cgtaatcaac gaaatactga gtggtatgaa
    22261 tcgggcttgt cgtatagcac atcgccgctt cgacttttcc gccgtatgct ttggtcgcaa
    22321 gaattgccat ccaaagatta cgaacatcat tcatcgcatc aaaaattcgc atattcttac
    22381 taccgctttc gatactttgt cgaatgaatg cggctaataa atcatccgga taaggactat
    22441 acgcaaataa gttttggccg cggagaagag attgaatttt atcccctcca cccatttcat
    22501 cacgaaatcg ttcaagtcgt tcccacgggt catctttggt gaatcgaatt gccgaatcta
    22561 aggtcgcccc accccaaagt tcgaacatgt tatatcccgc attttttatg atgggaagga
    22621 cacgcatagc ctgttcggtt gtcatacgag tcgccgcgca actttgatgc ccgtcacgca
    22681 acgttaaatc attaaaaaga actttttttg tcattatgct ttttctccgt tgtttatttt
    22741 tttcttttct atatttattg atctcttata ttatttattt actaaaatta tcatattatc
    22801 tgtatggtaa aaaaaactaa tccgtataat tctatcatat atccttggga atagttgggc
    22861 tctttccgtt ctttcttgaa agaatataga tagaaattcg atcttttaga aatgttgatt
    22921 gaaagagttt ttatttataa tatctcctac gggtccggat cgaaatcaaa gaaacaaacc
    22981 attcaaatca tacggaggga taattcttaa atcaataaat tattgttcat taaatctgct
    23041 tgacacctaa ttcttaaagt caatataata ataataattg ttcgattctt tatattcttt
    23101 aaagaatata tggaggagat cttttatggg tttctgacaa atgaaaattt ataacttccc
    23161 atttcttttt ttacgagtat tatcaataag agaacataat tcagaccatg ttaaacacaa
    23221 atcggaggat atggaatgaa aaaacaaata gatctgcaac aatttccggc tggaaatcta
    23281 cccgcacgta ttttaatggg tccgggaccg agtatggtcg atccgcgagt atatcaggca
    23341 atggcagccc ctttagtcgg ccatttagac cctgaatttg ttcatttgat ggatcgaact
    23401 caggaattgc ttcgatatac tttccgtacg gaaaacaaat taacgattcc ggtatctgga
    23461 accgggagtg ccgcaatgga agcttccatc gccaactcgg ttgaaccggg aacgccggtt
    23521 ttgatttgca tcaacggtta tttcggtgag cggctcgccg atatggcgaa aagatatggt
    23581 ggagatgttt caacgattca tcgcccgtgg ggtgaagttt ttaccccttc cgaaatcgaa
    23641 caggctctac gggagaagcc ggcaaaagtc gttgcgattg tccatgcgga aacttcaacc
    23701 ggcgcggaac aaccgctgga tgaaattgcc aaaatcgtcc ataatcacgg agcgattctg
    23761 atcgttgacg cagttacatc cctcggaggt gtaccgttga gtgtggacga aatcggaatc
    23821 gatatttgtt acagcggaac tcaaaaatgc atcagtgcac ctcccggatt gggaccgata
    23881 actttcaatg aaagagcttt gaatttaatg caatccagaa aagcaccggt gaccagctgg
    23941 tatctggata tgtcacttgt tcaagcttat tggggtccta accgaaccta tcaccacacc
    24001 gcacctatta ctatgaatta tgcgttatat gaggcacttc ggattatcgc ggaagaggga
    24061 attgaaaatc gctgggcacg tcatcgggca aattcggaaa tgttttggga aggtttagag
    24121 gatttgaacc taaagtgttt tattccgaaa gaacaccgtc tcacatctct ttgcacgatt
    24181 cagattcctg aaggggtgga tgatgccgcg gttcgaaaat cacttctgga ggattacaac
    24241 atcgaaattt ccggtggatt gggcagcctg aaaggtaaga tctggcgggt cggcttaatg
    24301 ggatatacct caaggaaaga gaatatattg ctgcttttgg ctgcattgaa gaaaatactt
    24361 tacaagtaat ttatcttttg ttgaaatttt aacaaaaaag aaggaggtgc atcttagtct
    24421 gatttcgatg cacctccttc ttttattcaa atttttgtag aatcgtcccc gaaagagaaa
    24481 agattacagg aatccccaat ccatatccgc tccttcttcc atcatttcgg gatcccatgg
    24541 atcaaagcgt aaatccacat aaacatctgt tttcaaagcc tccattgctg ccattcgcac
    24601 ttgttcgatc atatgtcctc cgtaagggca gaaaggagtc gttaaaatca ttgtgatgac
    24661 agcacggttt tcttctttaa cgacatcacg gattaatccc aaatcaatca cactgtatcc
    24721 caattcgggg tcgtgaacat tccttaatga ttctttcaat aattccgctt cctttggatc
    24781 cgttttttcc aactgccagg aagcttttcc ttgcatgatt gtttcttcac tcatattgat
    24841 ttccctttct tgtgcttttt tcctcatcgg tcattttcgg gtatctgtca gcacccccat
    24901 ctcaattaat gcccggcgaa cagcttcttc ccctttcggt tttatgaata cggtattggc
    24961 attcagctgt tgttgaatcc attgtgaagt gtcttttgaa tttaataagc tggctatcca
    25021 atcaggattg gttacggtta atacagtcgc ttgaaacatg gtcgcttgtg tatccattcg
    25081 atcccattgc tcaacggcat tcaacaaggc ttccggcaag gctttctcac cggacctttt
    25141 caacatagcg ataaatgaag gtacggataa tccgttttct ttagcatgaa gcaatgattc
    25201 gggggtaact cggaagatcc accgatccgg ttgaattttt tcccattcgc agaatcttgc
    25261 gatatggtaa cgcaatatcc gcggtacatt cggatttacc gaaattctcc cgtctatggt
    25321 gactttgggc ggtacttttt caagattcgc ttttctataa ttgtcttttg ggacttcctt
    25381 ccccaaggat tcagccaaac atctttcacc gatcctgctg accctaaaag cactgattgg
    25441 tagaaaacca ttcggctttt ccggattttc acttttgggg gcatttgaag ctatatccac
    25501 aattcccaac caatgaagcg gtcccgaaag caaatatcgg aggtagattc cttcgacatt
    25561 ttcccattct ttatattgat ttttctcgcg attacgaatc aaccacgccg tggaatcatt
    25621 ttgatttcgc aaaaaatcag gcgaatcctg ttttactgcg gaaagaaaac ccgaaaagct
    25681 ccaccaaccg ttgccatgaa tccgattaag aaattgcatt acatttgttc gtagtttact
    25741 cggattgtga gcaaataatt catcgacagc caattcctta atccatagca attcatccat
    25801 ttcctcggtc tgctgccagg ctcggatgag ttgaagcaaa gcactaagtc gatctatcat
    25861 taaaaaagat tgacctgttt ccgctatcgg cataaaagat tggtccaata taccggcgat
    25921 ttgcagtaga ttaaccgtaa atcgagtttt tttctccccg taaaccggat aaaacaacga
    25981 aaactcatgc ccggtacgca acgaagctaa gatcaaacaa ctgagatcca agacggaatc
    26041 atttcccgca taaatttgaa caacttcccg cggagcagcc ggacgaataa tcaaatcagg
    26101 tgaatttgat tcttcaatag gcaggttttt cagttcttgg cggaatttgt cataaagctc
    26161 atccggtaaa tagacatatt ctctcattcc ttccggggta tcaaaaaagc ttttttgtat
    26221 caatcctcga taccaaagcc actgcgccgg attttgcgga gatttccatg gttcttcacg
    26281 aataaatcgt gcttcaccca ttacttctat ctcgccgaat aaccgaaaaa aaggtcccga
    26341 ttgaatcttg ccgttgttta ttactaattt ttcaatcgca ggtctgacgg actccgggag
    26401 atctttcctt aaagagcttg aatcgattga ttgaataatt tttcgcgcaa ggatttcctc
    26461 gattggttcc gctgactcgg cagacgcgga tagattccaa ctggcagcaa tcgcatttag
    26521 aatataaggc tcgtgaagag atagactttt tttcagatca ggcattattc atcgctatcc
    26581 tttttgtcaa tcgacgccgc ttcccacgtt tggaccttgc tggaaattac cgccgtgacc
    26641 cattgtgcgg ataaataatc ctcttttttc tccccgatta caattaaatc ttgcggatcc
    26701 gggatcggct gatcaaaatc ttcttttaca accggaagag aaattaccga tactttccca
    26761 ttaagtaaca tccagaccag acgatcataa tcggcattca cttttatact cgaatttccc
    26821 cccgagtcac cggtatgggc ggtataaata cgcggataaa cagctcttcc ctcaagaatc
    26881 tcaagctgtc cgttttggat cttttgcgat aattggtttt ggatggcgag gtttttattt
    26941 tcgggagtca tttcgatcca tcgattcaat atttcctcgg atgacgatga cgaatgaaac
    27001 cgccttggaa taaccgaatc acagagcggc aaagccaacc ccaacagaat aattataaaa
    27061 gaaaggcata ttgacagcga ccgtttcgga aacccatccg ttatcgcaat ctgcgtaaaa
    27121 gcaggctgat ccgtcatttc ccaaatataa atgaaaggaa atcgcatcac cgcaacgcac
    27181 ccgatcgcga aataaatgag tgtcacccag tctaccggta aaataaaacg aaaacctgag
    27241 gtcattgcga aagcggttcc caaagaataa gtgagatgca gcagaaacgg aacggcacca
    27301 gcccaaaaag tccgtctgat cgcacttcca aatccgatgc tgaggataat aatataaaca
    27361 aaaagcgata acttattctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27481 gcgttcgcag catgagcgta aagtgaataa agataattta cttcggagtt gctttcgccg
    27541 gctgtcggat tcgggtgaat ttgattccaa acgttccacg gcaaaataat gattaatacc
    27601 gaaaccgcaa atattccgaa cgatttaata gtgtgcaaat tcaagcgctt ttcaatggaa
    27661 taacgaatca ggagaaagag caatacaacc ggaataaaaa ccgcggattg tgttctgatt
    27721 tggacggcaa ttccgcataa gccgcccgaa atgattgcaa acagagattg tcgtttttta
    27781 tctgcctggt accaatgaat caatgcaacc atcagccaac tcacaatcaa tgcagtcggg
    27841 agatcagaca tcatcagacg gctgttcgaa acttgtacaa caggggtcag ttcaatgctg
    27901 taaacttccc gcaggatgac ccaccaagcc gccaggatgc cgcttaacgg aaaatgaagc
    27961 aaagtaccga tccgataaac cgctacaggg aataaagcta atacgattat ctgtaaattc
    28021 gtcacagaaa ggtagttatt acccgcaatg gcgtgcaaga gtgctaagaa cgcggcataa
    28081 aacggccgca ccacacccat tccattccga aatccatcac cgatcagaat actctgtgcg
    28141 aacatatcat aatattccgc atcggaagag ggataaaaat tataattcgg gggtcttaac
    28201 gccggtaaaa aataacttct tccttcaaaa ggtgtcctga tccagacaat ggaagcgacc
    28261 agccagatga tcaaccctgt caatcggata aagttttttt gacctttttc ggatatcgcc
    28321 ttttttttca agaatcgact ttgcaaaaaa gtgatcaatc cgattatcgg gaaaagaatc
    28381 aacaaaggga aaatcaaatg cccttccagg attgagactc cctgtcgata aaatgttcct
    28441 gaaatgatat ggagtcccaa tccggttttt aaagcaaaca caatcagaaa aatgagcaga
    28501 attccgcaag ttactgccca gatcaacgtt tttcgattcc gattcagcct gctcttaata
    28561 tcttctttcg aagaaaaaaa ccatacaaag aaaatcgatt cgacagaaaa caaaccgccc
    28621 caaactaaag ccggaattaa ccttgtaaat aaagatttcg aaaaagccgt gattccgaaa
    28681 ggaggtcgca gaaatatcca tgctaaaact acgatatatc ctgagagtaa accaaccaaa
    28741 ccgacaacct gatatctgcg gttatctttt acgctatctg aggcatcttg ctcggtcaaa
    28801 gcattcttgg cgaatcgcag gcaaagaccg ataagaaaaa gcaatcccaa aaccagtccg
    28861 attctgagcg gcgacagacc gaacagaact gcactttccg cttcggatgc ggtttgaaat
    28921 atcatcacga cggatgctat tccctcaaat gcgtttacca gccagaacca gaaccatttt
    28981 tttgacacat tcatattata aaatgggtga ggttcttcat caaccttgag caaaatggga
    29041 atcatctctc cggtttataa agaaaaattt gagagatatt tatgaagtct taaacggctg
    29101 aaacggattt ttatccgatt gaaatgattc tcaaaaaaca tg
//
LOCUS       DAOSMM010000023        27483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1749_length_27483_cov_16.583710, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000023 DAOSMM010000000
VERSION     DAOSMM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 27483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27483
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1749_length_27483_cov_16.583710"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PK898_05115"
     CDS             <1..393
                     /locus_tag="PK898_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19449.1"
                     /translation="GDTYIGPVSPTQALTYQFIKSGYYEPNLKKIKELYAPRLAKALE
                     VLDRELPGAVYPRPEGGFFIGVTLPEGNDMATLIPRAKEAGINITDGRGFYLNPEDGK
                     RFLRIPFCGLKPAEIEEALGQLIPLLVK"
     gene            complement(456..1787)
                     /locus_tag="PK898_05120"
     CDS             complement(456..1787)
                     /locus_tag="PK898_05120"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOU19450.1"
                     /translation="MDEKTLFERIAQEQVKFISLQFTDVFGTVKSVDLPVSKLHEAID
                     QGIWFDGSSVEGFARIQESDMHLRPDIDTYVILPWTPEEIRRARIICDVELPDGSPYM
                     EDPRGLLKRACAKLAEQGMVFNVGPEPEFFLFRRNGDKMHPVPFDTGRYFDFSADDTA
                     VRVRTKLILALNEIGLDVEVGHHECALGQHEIDFKFADALKTADNIQMMKSTVKAIAA
                     QEGIIASFMPKPVFGENGSGMHCHQSLSASDGQNLFFDPNDEMYLSDMAKYFIAGQLK
                     HAKGMTGILAPCVNSFKRLVPGYEAPVYIGWAQTNRSALIRVPSTAGKEKASRVELRC
                     PDPSCNPYLALTVMLAAGLEGIREKVLPPVPWNNVNVYELSRKERRKNGIEELPGSLK
                     EALLALEEDTLIRSVLGDKLFDSYIRGKWEDWDEYRISVSEWEVDRYLETA"
     gene            complement(2024..3310)
                     /locus_tag="PK898_05125"
     CDS             complement(2024..3310)
                     /locus_tag="PK898_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOU19451.1"
                     /translation="MKKFFGSFVKILPSIILSLLLSITVWVIAVTEKDPTEERRFPNT
                     INIEVVGLSDDLVMTNSLPEQIYLKLRAPSSVWDKIVNDRVQATAVIDVTGLREGKHQ
                     VPVQIKLPVDPISIISYSPETAEVDLEKYETREFEVKVTEMGEIPTAFKGETPVVSPK
                     TVRVSGAVSVLDQIDYIRVILNHSNATETISKDLPVGAISLSGKVINSGLTFDPPQVH
                     VTQEINLRGGYRVVVVKVVTEGSVPDGYQVAKISVDPSVVTIYSSDRTLLESLPSYVE
                     TDPILLSEYTGDASLKIGLNLPEGISLVGDQSVKVDIQIPPIVSAKTFSNVPVYVIGL
                     DTDQRAQISPETVEIYLSGPQPILNNVKADELYAVIDLQEYSAGHYQLTPRIDLASWE
                     GVSVQSINPATIDVTITGGKPATPAPAPTVTATVQP"
     gene            complement(3307..4194)
                     /gene="cdaA"
                     /locus_tag="PK898_05130"
     CDS             complement(3307..4194)
                     /gene="cdaA"
                     /locus_tag="PK898_05130"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOU19452.1"
                     /translation="MQQLISELLFLGQRFNRVSVIDILLVASIFFVILRFIRSSQANT
                     LLRGTITIFIIIALLYVLVDLPAFTWLIGNILPVLLIVIPVVYAPEIRRAFERMGRVQ
                     SIHDLFASAPPHAEEMNQVIDAVVTACSMLAARNHGALIVMQRYDDLEKFIETGVYLN
                     AVVSPELLLQIFYPNTPLHDGAVIIRKGRIEAAACVMPLSSRNVLDKTPGRSMGLRHR
                     AALGTSEANDSITIVVSEESGTISVAFEGKITREISSERLKSMLENVYEQPEETPFMQ
                     RVGKFFDFIEKFLLGGEKK"
     gene            complement(4337..4933)
                     /gene="coaE"
                     /locus_tag="PK898_05135"
     CDS             complement(4337..4933)
                     /gene="coaE"
                     /locus_tag="PK898_05135"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOU19453.1"
                     /translation="MKKLVIGITGGIASGKSEIRRELESLGCLGIDADRIGHEVLEPE
                     SSVADDVLRHFGSRIRSDRNTGKIDRKKLGQIVFENPTELKWLESVTHPQIIRRINQI
                     IAETDRSVALEAVKLSDSGLSSLCNQKWLVETDPEIQTERLMRKRGLSREDAQIRIRM
                     QQNMDWHRNEMDHIFNGGIPLADLKAEVRKVWYALQDH"
     gene            complement(4957..6294)
                     /locus_tag="PK898_05140"
     CDS             complement(4957..6294)
                     /locus_tag="PK898_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU19454.1"
                     /translation="MNLTGKNLEFKHKNRSDPGVVIMLMFLCLFSFFLLKGVLTGQIV
                     SPWKPTAIPTRDAASYAMEAEAQFVAGNLEKAVEAYQAAANLEPTNPEYIWKQGRALA
                     YMTASQTTDMEKKETLEKAIKLLESSLETFKENSDIYAVLAMVYLWYADKDICGADMV
                     ETNMATAESYIRRAVDYGPRNAMAYAFYSEILLDQANYERAEQMLSQALEYGTANGSD
                     MFDVYRVQGNLLESYGQYRSAIDAYNRALALSPNMTFLQIRIGVNWRQIQEYDAALEM
                     FSKAARINDQLGINDPLPYLAIGNTYSQTGDFYAAGINIKKALSINPYNADVYGRLGV
                     NYYKARNYEAAIEALKCATYGCGAVESCAVRECDDPGNPLIEISGLPLSGTSVIYYFT
                     YAAALSYMHTNANHYCEEAVKITKEVSKQFGTDETVMSIVRDSEAICASYGIK"
     gene            complement(6316..6939)
                     /locus_tag="PK898_05145"
     CDS             complement(6316..6939)
                     /locus_tag="PK898_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19455.1"
                     /translation="MRKRITILTLIGMAICSFIYPVSAEDRPPVAPDMAQYALNLMAV
                     ECQNLSDFAGQAAQNNFGNFDSVKSQFEVAISNFNYLIGPFIPEFIQEFNSAYQNFEP
                     SVSSAQQTAQICQNIRTNTYQKMTQYNGILNPPQDIDTFEKCEQAGYFISGDTCFIGG
                     EYILDKKGYLIGIYNASCFVGDTFYGGTCWYCVYGNDENGCLERRDR"
     gene            7181..7984
                     /locus_tag="PK898_05150"
     CDS             7181..7984
                     /locus_tag="PK898_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU19456.1"
                     /translation="MKHCLNCYRKIPNDAKICHYCGAPQAEKEAFPGKKFLRCPNCMS
                     YFYDEEHGCQNCGYSPKKPKKKGTWLLAVLFVLIAVFIFWQLGIIPDLSKSEKKADVV
                     QTILSTADEQPPAEIETSEMEADVLSADAIEIGRLTEEAVPDASVAETSATAMNSPVP
                     MTATPAPFQCNGIDNRLMKGMTGKIIAGGNPVKIRMQPSTGGESVSVLYGDELFTVLD
                     EKPVCAEGYLWIKIKLEKADLEGWTVEAADQNYWLMPLDLTAATETPAD"
     gene            8079..10562
                     /locus_tag="PK898_05155"
     CDS             8079..10562
                     /locus_tag="PK898_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOU19457.1"
                     /translation="MATNDSEKFYLGKVYDEQGNLTDEKLLYDPQDLVTHCMITGMTG
                     SGKTGLGVILLEEMARKNIPAIVIDPKGDLTNLMLHFPELRAEDFEPWIDPEAPVREG
                     KSLAEISGETAEAWKNGLSEWGLGKEDISELEKVSYTIFTPGSTVGNPINILSSFEAP
                     ETPVKEGDEFIREEIATTVTALLDLIGISNIDPLQSREHILLSSIIEQYWNMGKSIDI
                     EEMIAAVNDPPFEKLGALPVDKMYPPKDRFQLSLLLNNFLASPSFQNWNKGPNLDIGK
                     LLYTNDGKARMNVFYLQHLNDHERMFFVTMLYSQIESWMRQQPGTGNLKLGVYFDEIA
                     GYLPATSNPASRNVIIRLLKQARAFGVGMILSSQNPIDFDYKALSNAGTWFIGHLQTA
                     QDVKRLIDGLATNDGGIDQTEAKRRISALKNRQFLYMNVHTPGLKVMTTRWTLNYLAG
                     PLTRNRIPLLIEKGLSERVDTVSPVMNVNEAVAELADTSQNQEEDQGEAKMSEEKAKE
                     VQPEGTKPEIAANINEYYLRAEKTAAEVGAPAEEITYLPAWIAQAEARILQPKYNLDF
                     IDKKAVLIDEPDIRGVSINWREYETEPFDEKTVQSRPQYADAKYEQPPEWMSDSGSVR
                     SRESDFLDYIYREGIVKIRANKPLDVYAAPDVTEEDFIAMCKGKVAEASQNEKDKLAK
                     AYENIVDRMETKIRKAEADVADKTDKVKSKNIEKYGAFGELVIGMFTGRKRSVSTSIN
                     KYGQSTNADNALEKAQLVLDELKADLEQQTEEFKKQIAEVEEKWNKIAEETDEVTVTP
                     MKKDIFIDYFGILWLPYYTDENGKFVKAF"
     gene            10707..11558
                     /locus_tag="PK898_05160"
     CDS             10707..11558
                     /locus_tag="PK898_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19458.1"
                     /translation="MDIEETQPIRPKRGNPYVTFPDFSPSVPAEPGIAVPTELNRESE
                     GRFVPPPYPPVPVSPEMAKALSAEIKSRKIQFLTHFTRLENLASILTYGLLPPNQLKS
                     EGRTRFVRFNPPYLPGYWQNAISFNISFPNYRLFYNLQEQRGYDWVVLLFDINILLSQ
                     PFYYFIYPAANLIHTPIFATEISPKLQTFEAFEELFQDTENVRRAFLQIPDCYPTHPQ
                     SEVLTFQPVSVNALLEVHFYNDYKFNQWFMQNTALAMTMDKNIWQVSLEFFSPRCDYL
                     NWKSTQR"
     gene            11671..12231
                     /locus_tag="PK898_05165"
     CDS             11671..12231
                     /locus_tag="PK898_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19459.1"
                     /translation="MTCDLQFIQSINQGFVEILGKEDLDDFRLFLKEPSESESCMRYL
                     SMMQCYLSEKFGYFESEGIAISIGKNAYRPIKIAFESVLHFQEVDFRLLPHHKRIKTA
                     LVRLIERVYKPVGIPIMSLDTEETDSWAIKPEGPIRMFPVQILNGILQEMLLDVSGGH
                     FFPVEVQEDEQRNMIILTFATSPIGH"
     gene            12308..13354
                     /locus_tag="PK898_05170"
     CDS             12308..13354
                     /locus_tag="PK898_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU19460.1"
                     /translation="MKKFLPLVLTILILFCFFSVQADKNVLNVLTYNSFAVSEDVIKD
                     FEAENNVKVQFIEGGDTPSIVNRAILTKNSPIADVIVGMNDVLLARVLKNEILEPYES
                     PELKNIPDEFKSDPAFGALPFDYGHVCINYDTAYFTDHQLAVPSSLDDLISEPYKGLL
                     VVENPTSSGPGQAFFLTTVAEYGEDGFVDYWNKLLANDVQIDNDWSSAYYTSFTAGGG
                     NGPRPMVVSYDTSPAAVPFYSETPMETAPTAAVLADGTCYRTIEFLGILKGTKNREIA
                     EKFVDAMLGKRWQEDLPGQMFVYPVNREAVLPEVFAKFAIQPENPVSLDSALIEEKYE
                     DWIQTWNDEVLVNY"
     gene            13377..14333
                     /locus_tag="PK898_05175"
     CDS             13377..14333
                     /locus_tag="PK898_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU19461.1"
                     /translation="MSIVCSGSVAYDYLMRFPGYFRDQILPDHLDSISLSFLVDDMVR
                     LQGGVAPNVCYSLALLGEKPILFAAVGEDFDQYRKWLEERGVDCRFARVIPGKFTASF
                     FSNTDLANAQIASFYTGAMKNSADLKVSELKDEKVDLFLISPSDPDAMIGYARQCKEL
                     GIPFGFDPSQQIVRMNREMIREGLDGAKFLFCNEYEFELLKKHSEWSEEEVLDRVDCA
                     VITFGEKGSRIYADGSVTTVPVYPPERIVDPTGVGDAYRSGFLTGYYKGLDWELCGKM
                     GALASTYVLEQKGTQSHRYDRETFIERFRKLWDDGGRLDCLL"
     gene            complement(14455..17049)
                     /gene="mutS"
                     /locus_tag="PK898_05180"
     CDS             complement(14455..17049)
                     /gene="mutS"
                     /locus_tag="PK898_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU19462.1"
                     /translation="MSEEFTPIRQQYLEIKKQYPDTIVFFRLGDFYETFDEDAETTSR
                     ELDLVLTSRNVAKGVRVPMAGIPYHAVENYLSRLIDKGYHVAICEQIGDQPTKGLFER
                     EVVRVVTPGTLIEPGLLKSDSNNYLMAACVENDKIGIAYADITTGEFAVTQIQSADVT
                     ADLQSEITRLNPAEILVAQSFDLPLSENLHITRVPDYNFEPLRCTELLRDHFQVATLD
                     GYGLKENSIPVRAAGCLLQYLQDNQPSSIKLLSSISFYSLDDFMVLDSSTRRNLELTE
                     SLRNNELKGSLLGVIDCTVTPMGKRVIRQWVSKPLTNQNGIQNRLDAVESFFNDGMIR
                     AEVRESLKGMNDMERLINRISGGMAIPRDLTALRSSIRKLPEIIEIIEPILPALTPII
                     QKIYPLEVVKQILESAVADDPPATLMNTGVIRAGYSEELDRIHQESGHAREWMANLES
                     SERERTGIKTLKVSYNKVFGYYIEISRGSADQAPPEYIRKQTLVNAERFITPEMKEYE
                     ALVLNAEERIREIENRLFYQICAEISQYATEILTTARGIATLDVLAALAESAVRNKYV
                     KPEINNSLEIEINEGRHPVVEQTLPQGTRFIPNDIKLGGNSFIHVITGPNMSGKSTFL
                     RQTAIIVLMAQMGSFVPASSAKIGLVDRIFTRIGAQDEIHAGLSTFMVEMVEAANILN
                     NGTDRSLLILDEIGRGTSTYDGLSIAWAIIEYIHNAPGLKSRTLFATHYHELTSLANS
                     LPGVKNYNVAVTENAGEVVFLHKIIPGAADRSYGIHVAQIAGLPKPVISRANEILKQL
                     ESDSVKQMEIRETPSYQLTLFPETNPIIDTLDRLDVNSMTPIEAINILYEWKQRFGKS
                     DSNKKKTG"
     gene            complement(17046..17693)
                     /locus_tag="PK898_05185"
     CDS             complement(17046..17693)
                     /locus_tag="PK898_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU19463.1"
                     /translation="MKHQTRLCINPDCTLRFTIPLSENISIRCPRCGSGTIPIDEGYD
                     SNLIPQRKQDESGLPTVVAVLDNIRSALNVGAILRTADGAGIRKIFLCGITPTPENPK
                     VLKTSLGAENAIRWKHSWNILEVVEQLKSEGFLILCLEGGKRSINLFQNIGKILSGQP
                     VALFIGNEINGIDPVVLEKADFSVFLPMQGEKESLNVATAFGIAAYLLRYGKTIK"
     gene            complement(17843..21076)
                     /locus_tag="PK898_05190"
     CDS             complement(17843..21076)
                     /locus_tag="PK898_05190"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HOU19464.1"
                     /translation="MNKTVVIASDHAGFELKQNIIETVEEAGCKVIDVGAFSKESSDF
                     PDFVQLGAEKIKSGKADAGIFLCGSGVGVCIAANKIPGIYASVCHDTYSAHQGVEHDG
                     MNVLCLGARIIGPELANELVKAFLGAAFNNKPNQIRRFNKIKQIEAGDLYLINRIEAL
                     RDIGQSVWLDQANRCQLKNGELVKMIEKGQVSGLIMNSGNMCSAILNSDDYASALVPM
                     MLAGWNCRKIFDKLLVDDVRSAAGIFKDLYISSSGKEGYVTLTLDPELSHNIEKTVSE
                     TKRIWTAVNRPNILIGIPATEEGLEAAKQLVTEGININFTMIVSEKSYSGAVDAYLSG
                     LETRLKEGKPIDKIYSTASVDVAEIDHIVNKFLDQAVDPVDPDKSTATRRLKMKAALI
                     ICRRIFNLFLETNQSGRMQNLIAEGAEIQHLLWSSLCHKEPSFKDTYYTDSLIAENTI
                     LSVHPKLMDAVIDHGQIIPSLRVPHEEDNQIIEEFEKLGIQFESLTERVQEEIMESYK
                     SAFESMLKELDKKSDRVKKSLGCLYTPVFENFKKLEEDSIIQRIFAKDPTVWTFDTQA
                     YPEIRNRLGWLDAHQNIAKSIDEYKSIAAGLKKDGIHKILLMGMGGSSLAPEVMALTF
                     KNNDGMALEIIDSTDPVQVLAADASHPPKETVYIVSSKSGGTAEVQAFLDYFFERAKT
                     ELSDQAGSHFIAITDPGTQLERKAKEMGFRNIILADPSIGGRFSALSAFGVFPAVLMG
                     INPETIIEQATRMAKICAPSNSVGCNEGAALGVYMGTATLNSRDKFTILTDPEFSAFG
                     SWLEQLIAESTGKMGKGIVPIDIEPKLAPSEYAEDRAFVYVETSGTQTDFVNELITAG
                     QPVLTIKLNDLNELFAEFYRWEIAVAVACSLLGVNAFDQPNVQDSKNRTVAKITEYKE
                     KGQLTDFPACWEKDGLSVYFNTDIPDLKQADDIKHLIKAFIRQAIKGKDYIAINAYLP
                     RNEKMAADLQNVRAWILKETQCATTLGFGPRFQHSTGQLHKGGANNGLFIQLIADAPQ
                     DIEIPNEGITFSILEKAQALGDFEALLAKERRAIRINLGSKAVTDLL"
     gene            complement(21122..22150)
                     /locus_tag="PK898_05195"
     CDS             complement(21122..22150)
                     /locus_tag="PK898_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOU19465.1"
                     /translation="MKALVLTDYKKMEIQDVPVPIIAEDEVLIQVKACGICGSDIHGY
                     DGSSGRRQPPVIMGHEASGIIVETGAKVLGWKKGDRVTFDSTIYCGHCSYCREGLINL
                     CENRRVLGVSCDEYRRDGAFAEYIAVPQHILYALPDEVDFNQAALVEPLSVAVHAADL
                     SPHQLDASVTVVGTGMVGLLVIQALRAYGYGKIIAVDVDSKRLELAKQMGADDTVISD
                     ENALEKIRMLSGGEGTDAAFEVVGADATFNLAVNSVHKGGSVTLVGNISQKTNFPLQS
                     VVTRQIRLQGSCASSGEYPACLNMIARGSFNVNPIISATAPLSEGPEWFDNLYYQKVN
                     ALKVILNP"
     gene            complement(22169..23311)
                     /locus_tag="PK898_05200"
     CDS             complement(22169..23311)
                     /locus_tag="PK898_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU19466.1"
                     /translation="MDKIRTAVIGCGKVGATHALAYKTLPNSELCAVCDVSQKNANQF
                     AKDYNVRAYSDIKKMIRENGVQVVSVCTPHPLHAEPTCEAAEAGANVICEKPLASDLR
                     DCDRAITACEKAGVKLGVISQRRFYWPVLRMIQAIRDGKIGKPIIGTIEVMGWRSPEY
                     YQMDGWRGKWREEGGGVMVNQTPHQIDLLQWMMGDIDELFGYWDNFNHPEIEVEDTAL
                     AVIRFKNGGIGQILVSNSQKPGLWGRIHIHGSNGASVGAQVEGGSSFISGVTTSKVEP
                     AFNDIWTVPGEENMPEVWKKEDQIKSEGIDPMTYFHQIQIADFLDAVIHNRPPSVDGH
                     EGRKTVEIFTAVYRSQRDHRPIKFPLSAEDGSDFDGRLSYVPYSHR"
     gene            complement(23356..24834)
                     /locus_tag="PK898_05205"
     CDS             complement(23356..24834)
                     /locus_tag="PK898_05205"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOU19467.1"
                     /translation="MTAHNLREKYDSRNLSMLFDFYEMTMGNGYFKQGMKDKIVIFDL
                     FFRRIPDDGGFAIICGLEQVVQYIENLHFTEDELAYLTNKKLFDPDFIDYLRHFEFHC
                     DVWAIPEGTPVFPNEPLVRVRGPIIEAQLIETMLLVTINHQSLIATKTNRIVRTAKGK
                     TVLEFGTRRAQGYDAANYGARAAYIAGVAGSANNWADIHLGVPASGTMAHSWILSFDS
                     EYEAFKAYAKSYPDNCNLLVDTYNTLKSGVPNAIRVAHEVLEPMGKRLVGIRLDSGDI
                     AYLSKKARKMLDDEGLKDCKIFASNSLDEYIIRSLNVQGARVDVYGVGERLITASSEA
                     IFGGVYKLVGIYDENGNLIPKIKISDTFEKITNPSFKEIWRLYEIGDNHPLADVITLE
                     DEVIEDNVPIEIFDPLDPTKRKVITNFHTKKLLVPIYEKGKLVYKLPTLQQIRRNCQK
                     NVDELWEEIRRLDNPHTYYVDLSQKLWDLKTQMIKEARMISQ"
     gene            complement(24834..25406)
                     /locus_tag="PK898_05210"
     CDS             complement(24834..25406)
                     /locus_tag="PK898_05210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOU19468.1"
                     /translation="MQNLLVIMDMNNGFAKKGNLFSPRTANLIDPIADYCKKSKEKGW
                     TILALSDTHDPQDIEMESFPSHCLAGSDESEVVDEIKPYCDFILPKFTTGGFFEIQEA
                     SQENKSLDLRRYEEIHVVGCCTDLCVFNFCLITQKHMEYEYHKKRISRIPDIIVHKDL
                     VDTYDSEGHEADNLNRFFWEHMKLNGIKVE"
     gene            25729..27354
                     /locus_tag="PK898_05215"
     CDS             25729..27354
                     /locus_tag="PK898_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU19469.1"
                     /translation="MLQDIRVRQRDYLIELARVLTQEMDLDVLLWKILRITLNLLNGE
                     AGFIALYDSKEKWHVRAISGVLEPTARYIETYLKNFSEREESFDQNALIEINLLIQRI
                     CQMPELGFADGVGLPLVEHGNFLGIIVIFRKFHTGFTINDLAMLKIFADQAAIAIHNA
                     DLYGENIREKNRINALFDAVPDGIFVLDLSHHIIRVNPAMTKLLHVKEETLLGEHHDE
                     VIVLENITAGMELSAGEAGGWPFSKESKLYVEGNLAYGPLKEKPLPVGIAYSPVMNDE
                     NTMINIIGLVRDISKFREADDLKNTFISTMSHELKTPVALIKGYASTMQLDDAKWDKD
                     LIKESFAIIEEEADRLANMINSLLDASRLYAGALTLNRSEINIAELARNIAKRMQTQT
                     DKHTIRCEFPADLPIIYGDADRIEQVLMNLISNAIKYSPGGEILISGELQNDFVKISV
                     KDEGQGLQSEDLQHLFERFYRSKRSANLAKGVGLGLYLSNGIVEGHGGKMWAENRTDR
                     SGAIFSFTLPLNPDDHPAALKFRDIGDYKNHSL"
BASE COUNT         7348 a         6174 c         6279 g         7682 t
ORIGIN      
        1 ggcgatacct atatcggtcc ggtcagcccg acacaggctt taacctatca gttcatcaaa
       61 tccggttatt atgagccgaa tctcaagaag attaaggaat tgtatgcgcc gcggctcgcc
      121 aaagcattgg aagttttgga tcgagaattg ccgggcgccg tataccccag accggaaggg
      181 ggctttttca tcggcgtgac gctgcccgaa ggaaatgata tggcaacgtt gattcccaga
      241 gcgaaggaag ccggaatcaa tattaccgac ggtcgcggtt tttacctgaa tccggaagac
      301 ggaaaacgtt tcctgcgaat tccgttctgc gggctgaaac cggcggaaat cgaagaagct
      361 ttgggacagt tgattccgct gctggttaag tgattggatt ccatcaaaaa acaccgttca
      421 gcgaacggtg ttttttgatt ttctgccaga tttgtttatg cggtttccag ataacgatcc
      481 acttcccatt ccgaaaccga gatgcgatat tcgtcccaat cttcccactt gccgcggata
      541 taggaatcga acagtttatc ccccaaaacg gatcggatca aggtatcttc ctctaatgca
      601 agcaaggctt ctttaaggga gcccggcagt tcttcgattc cattctttcg gcgttcttta
      661 cgagagagtt catagacatt gacattgttc cacggcaccg gtggtaatac tttttcacga
      721 atgccttcca atcccgctgc gagcatgacg gtgagcgcca aataaggatt gcaagaggga
      781 tcggggcagc gaagttccac ccgcgacgct ttttcttttc ccgcagtgga ggggacacga
      841 attaatgcgg agcggttggt ctgcgcccag ccgatataaa cgggtgcttc atagcccgga
      901 accagtcgtt tgaaactgtt gacgcacggt gccaagatgc cggtcatccc tttggcatgt
      961 tttaattgcc cggcgataaa atattttgcc atatcggaga ggtacatttc atcgttcgga
     1021 tcaaaaaata ggttttgccc atccgatgcg gaaagggact ggtgacaatg cattccggaa
     1081 ccgttttcgc cgaaaacggg tttgggcata aaagaagcga tgattccttc ctgtgcggca
     1141 atggctttta cggtcgattt catcatttga atgttatcgg ctgtttttaa cgcatccgca
     1201 aacttaaaat cgatttcatg ctggccgagt gcacattcat gatgtccgac ttccacgtcc
     1261 aagccgatct cgttcagtgc gaggatcagt tttgtccgaa cgcgcacagc ggtatcatcc
     1321 gcggaaaaat caaaataacg gccggtatca aaaggcaccg ggtgcatttt gtcgccgttt
     1381 cttcgaaaca agaagaattc cggctcggga ccgacgttga agaccatgcc ctgttccgcc
     1441 aatttggcac aggctctttt gagcaatccg cgcggatctt ccatataagg cgagccgtcc
     1501 ggcaattcga catcgcaaat gattcgggct ctacgaattt cttcgggtgt ccaaggaaga
     1561 atgacatagg tatcgatatc ggggcgcagg tgcatgtcgc tttcctggat acgggcgaat
     1621 ccttcgacgg aagaaccgtc aaaccatatt ccctggtcga ttgcctcatg cagtttggaa
     1681 acaggcagat cgacgctctt aacggtgccg aacacatcgg tgaactgtaa agaaataaac
     1741 tttacctgtt cttgcgcgat acgttcaaat aatgtttttt cgtccatatc attttctccg
     1801 ttcctcaatc gatatccgat tgatttgact gtacgggatc gctttagcga ttcccgtatt
     1861 aaaaatacgt aggaattata gaggttttaa atattcggat aaaaaaacaa aattaaaata
     1921 aattacaacc aattttaaga ttttcaaaaa gagcgaagtg aagcgtaaaa aaaaccgctc
     1981 cgaattgatt tagctccgga gcggtcggat tgattcgctt tcgctaaggc tggacggttg
     2041 ccgtgacggt aggggcgggt gccggcgtag ccggtttccc gccggttata gtcacatcga
     2101 tggtggccgg attgatcgat tgaacggaaa cgccttccca ggatgccaga tcgattcgcg
     2161 gcgtcaattg ataatgcccg gcggaatatt cctgcagatc gattaccgca tataattcat
     2221 ccgctttgac gttgttcaaa atcggctgcg gcccgctgag gtatatttcg accgtctcgg
     2281 gtgagatttg cgctctttga tccgtatcca aaccgatgac atagaccggt acgttgctga
     2341 atgttttggc actgacaatc ggcggaattt gaatatccac cttgacggac tgatcaccga
     2401 caagcgaaat cccttccggc agattgagcc cgattttcaa ggacgcatcg ccggtatatt
     2461 cgctcaaaag aatcggatcc gtttcgacgt aactggggag gctctccaat agggttcgat
     2521 cggaggaata gatggttacc acgctggggt cgacactgat tttcgccacc tgatatccgt
     2581 ccggtacgga tccctcggtc acgactttga cgacgaccac gcgataaccg ccgcgcagat
     2641 tgatttcctg cgtcacatga acttgcggcg ggtcaaaggt caggccggaa ttaattactt
     2701 ttccggaaag gctgatggct ccgaccggca aatctttgga gatcgtttcc gttgcgttgg
     2761 agtggttcag gatgacgcgg atataatcga tttgatccag taccgaaacg gctccgctga
     2821 cgcgcaccgt cttgggcgac acgacaggtg tttccccttt gaatgcggtc ggaatttctc
     2881 ccatttcggt gactttgact tcaaattccc gggtttcgta cttttccaaa tccacttccg
     2941 ccgtctcggg ggaataagaa ataatcgaaa tcggatcgac gggcagtttg atctgaacgg
     3001 ggacctgatg cttgccttct ctcaatccgg tgacatcgat gaccgcggtt gcttgaaccc
     3061 gatcgttgac gattttatcc cagacggatg aaggggcgcg tagcttgaga taaatctgct
     3121 ccggcaggga attggtcatg accagatcat cgctgagacc gaccacttcg atattaatcg
     3181 tgttggggaa gcgccgttct tcggtgggat ccttttcggt gacggcgatt acccagactg
     3241 tgatggataa cagcagagaa agaatgatgg acgggagaat cttgacaaaa gaaccaaaga
     3301 atttcttcat ttcttctccc ctcccaacag gaatttttca atgaagtcga aaaattttcc
     3361 tacccgttgc ataaagggcg tttcttccgg ctgttcatag acattttcca acatgctttt
     3421 cagcctttcg ctgctgattt cacgcgtaat tttgccttcg aaagcgacgg atatcgtccc
     3481 gctttcctcc gaaacgacga tcgtaatgga gtcgttcgct tccgacgttc cgagggcggc
     3541 gcggtgcctc aaccccatgg accgcccggg tgtcttatcc agaacgttgc gtgaggacag
     3601 cggcatcacg caggcggcgg cttcgatgcg ccccttgcga atgatgacgg cgccgtcatg
     3661 cagcggggtg ttcggataaa agatttgtaa caggagttcg ggtgaaacga ccgcattcag
     3721 ataaacgccg gtctcgatga atttctcgag atcgtcatag cgctgcatta caatcaacgc
     3781 tccatggttt ctggcggcga gcatggaaca ggcagtgacc accgcatcga tcacctgatt
     3841 catttcttcg gcgtggggcg gtgcggacgc aaacagatca tggatagatt gaacccttcc
     3901 catgcgttca aaagctctgc ggatttccgg cgcatagaca accggaatga cgatcaaaag
     3961 aaccggaaga atgttgccga ttaaccaagt gaaagccggc agatcaacca gaacatagag
     4021 caatgcgata ataatgaaaa tggtgatggt tccccgcaga agggtgtttg cctgggaact
     4081 tctgataaag cgcaggatga caaagaaaat tgacgcaacc aagagtatat cgatcacgct
     4141 tacgcgatta aatcgctgac cgaggaataa taactctgaa atcagttgct gcacattagc
     4201 ctctcatcct tttttttcaa atttgaaact ttccttacca ttgtaactat tttcaacggc
     4261 agtagtcaag gaaaatcaaa tctgtgatta aaagttcctt aaaaaaggaa gaatttcaaa
     4321 taaaaaacag aaaaaattaa tggtcttgaa gggcgtacca aactttgcgt acttcggctt
     4381 tcaggtccgc taacggaatg ccgccattaa atatatgatc catttcattc cgatgccaat
     4441 ccatattttg ttgcatccgt attcgaatct gcgcatcttc ccgtgaaagc cctctttttc
     4501 gcatcagtcg ttccgtctga atttccggat ccgtctcgac cagccatttt tgattgcaga
     4561 gtgaagacaa tcccgaatcg ctcagtttga cagcttccaa cgccacggag cgatccgttt
     4621 ccgcaatgat ttgattgata cgccggatga tctgcggatg ggtgacggat tccaaccatt
     4681 tcaattcggt aggattctcg aagacgattt gacccaattt ttttcgatcg atttttccgg
     4741 tatttcgatc ggagcggatc cggctgccga agtgacggag cacatcatcc gcaaccgaag
     4801 attccggttc gaggacttca tgaccgatcc ggtcggcatc gatgccgagg caacccagtg
     4861 actccaactc gcggcgaatt tcgctttttc cgcttgcgat tccaccggta ataccgatga
     4921 cgagtttttt catttccggt tgtttccctt ccgcaattat ttgataccgt aagaggcgca
     4981 aatcgcttcg ctgtcccgta cgatggacat gacggtctcg tcggtgccga attgtttcga
     5041 gacttctttg gtgattttta cggcttcttc gcaataatga ttggcgttgg tgtgcatgta
     5101 agacagagcg gcggcatagg tgaagtaata aatgacgctg gtacccgata agggcaatcc
     5161 ggatatttcg atcaaaggat tgccgggatc gtcgcattcg cggacggcgc agctttcgac
     5221 cgcgccgcag ccataggtgg cacatttgag ggcttcgatg gcagcttcgt agttacgggc
     5281 tttatagtag tttacgccca accgtccata gacatcggcg ttgtaaggat tgatgctgag
     5341 tgctttttta atattgattc cggcggcata gaaatcaccg gtctgggaat aggtgttgcc
     5401 gatggcgagg taaggcagcg ggtcgttgat cccgagttga tcgttgattc gagcggcttt
     5461 tgagaacatt tccagcgcgg catcatattc ctgaatctgg cgccagttga cgccgattcg
     5521 gatttgcagg aaggtcatgt tgggagaaag tgcgagcgcg cgattatagg catcaatggc
     5581 gctgcggtat tgcccgtagg attccagcag gttgccttga acccgataga catcgaacat
     5641 atcgctgccg tttgcggtgc catattccaa tgcctggctg agcatttgtt cagcccgttc
     5701 ataatttgcc tggtccaaca ggatttccga atagaacgca tatgccatcg catttcgcgg
     5761 accataatcc accgcccggc ggatatagga ttcggcggta gccatgttgg tctccaccat
     5821 gtcggcgccg caaatgtctt tatccgcata ccaaaggtaa accatcgcca aaacggcgta
     5881 gatatccgaa ttctctttaa aagtttccaa cgagctttct aacagtttga tcgctttttc
     5941 cagtgtctcc ttcttttcca tgtcggtggt ttgagaggcg gtcatataag ccagtgcccg
     6001 tccctgcttc caaatatatt ccggattggt tggttccaga ttggccgcgg cttggtatgc
     6061 ttcgaccgct ttttcgaggt tccctgccac aaattgagct tccgcttcca tagcatacga
     6121 agccgcatca cgggtgggga tggcggtggg tttccatggg gaaacaatct gaccggttaa
     6181 aactccttta agcagaaaaa agctgaagag acagaggaac attaacataa taaccactcc
     6241 gggatcggag cggtttttat gtttaaattc caaattttta ccggttaaat tcatgaaact
     6301 gtatgattat tccgcttaac ggtctcttct ttcaagacag ccgttctcat cgtttccgta
     6361 cacacaatac caacaagttc caccgtaaaa ggtatccccg acaaaacaac tggcgttgta
     6421 gattccgatc agatatccct ttttatccag aatgtactct cctccgataa aacaggtatc
     6481 gccgctgata aagtatccgg cttgttcaca cttttcaaaa gtatcgatat cctgcggtgg
     6541 attcagaatc ccattgtatt gggtcatttt ctggtatgta tttgtgcgaa tattctgaca
     6601 gatttgcgcg gtctgctgcg ccgatgaaac tgacggttcg aaattctgat aagccgaatt
     6661 gaattcctga ataaattccg ggataaacgg accgatcaga tagttaaaat tgctgatcgc
     6721 aacttcgaat tgggatttga cggaatcaaa attgccgaaa ttgttttgag cggcttgacc
     6781 ggcaaaatca ctcagatttt gacattctac agccattaaa tttaaagcat attgcgccat
     6841 atccggtgct accggcggac gatcctccgc agacacagga taaataaacg aacaaattgc
     6901 cattccaata agagtaagta ttgttattct ctttctcatt tctcactccg actactattt
     6961 cttacttatt atacggtatt gaagggagaa aagtttactt tttttaagaa tcttttaaaa
     7021 aagatcaaaa agcattttcg atcgtttttt ttattatgac atcgaaacaa tttttgccat
     7081 atgaattcct ttcgggtccg attacccgaa ggggagatat tattcacggt ttgatttttt
     7141 gacggcgaaa cccgatccat tcgatcttat aattccggtt atgaaacatt gtttaaattg
     7201 ttatcggaaa atacccaatg atgcgaaaat ctgtcattat tgcggcgctc cgcaagctga
     7261 aaaagaagct tttccgggga agaaattttt acgctgtccg aattgtatgt cttattttta
     7321 tgatgaagaa cacggttgtc aaaactgcgg ctattcaccc aaaaagccca agaaaaaagg
     7381 cacttggctg ttggcggtgc tgtttgtatt gattgccgtg tttatttttt ggcagttggg
     7441 gatcataccg gatttatcaa aatcggagaa aaaagccgat gttgttcaga ccatcttatc
     7501 gacggcggat gaacaaccac ctgcggagat tgaaacaagc gaaatggaag cggatgtctt
     7561 atcggctgat gcgattgaaa tcgggcgatt gacggaagaa gcggttccgg acgcatccgt
     7621 cgcggaaact tcggcgacgg cgatgaattc gcccgttccg atgacggcga caccggctcc
     7681 gtttcaatgt aacggtatcg ataatcggct gatgaaggga atgaccggaa agattatcgc
     7741 aggcgggaat ccggttaaga tccggatgca gccttctacc ggcggtgaat cggtgagtgt
     7801 gctttatggt gatgagctgt ttaccgtttt ggatgagaag ccggtctgtg cggagggcta
     7861 tctttggata aaaatcaaat tagaaaaagc ggatttggaa ggatggacgg ttgaggcggc
     7921 cgatcagaat tattggctga tgccattgga tcttacggct gctacggaaa caccggcaga
     7981 ttagattctg ggaaagtttc cccgattttc ttaagttctt atataatgaa atcgtataaa
     8041 ttcattccgg ttttaattga tttgataagg agaaacttat ggcgacaaat gattccgaaa
     8101 agttttattt aggaaaagtc tatgatgagc aagggaacct gacggatgaa aaactcctat
     8161 acgatccgca ggatttggtg actcattgca tgattaccgg aatgaccggt tcggggaaaa
     8221 cgggactggg agtcattttg ctggaggaaa tggctcgaaa gaacattccc gcgattgtca
     8281 tcgacccgaa aggtgatttg accaatctga tgctccattt cccggaattg cgagccgaag
     8341 actttgaacc ttggatcgat ccggaagctc cggttcggga agggaaatca ctggcggaaa
     8401 tcagcgggga gacagccgaa gcatggaaga acgggttatc cgaatggggg ctgggcaaag
     8461 aggatatttc cgagttggaa aaggtttctt atacaatatt tacacccgga tccaccgtcg
     8521 gaaatccgat caatatcctg tcttcctttg aagcacccga aacgccggtg aaagaagggg
     8581 atgagtttat ccgagaggaa attgcaacca ccgtaaccgc tttgctggat ctgatcggaa
     8641 tcagcaacat cgatccgctt caaagccgag agcatatttt gctttcaagc attatcgagc
     8701 aatattggaa tatggggaaa agtattgata tcgaagaaat gatcgccgcc gtaaatgatc
     8761 cgccctttga gaaattgggc gctttacctg tcgataaaat gtatcctccc aaagaccgat
     8821 tccagttatc tctactgctg aataattttt tggcttctcc gtccttccaa aattggaata
     8881 aagggccgaa tttggatatc ggaaagttgc tttatacaaa tgacggaaaa gctcgaatga
     8941 atgtttttta tctgcagcac cttaatgatc atgagcggat gttttttgtg acaatgctgt
     9001 attctcaaat cgaatcttgg atgagacagc agcccggaac ggggaatctg aaattggggg
     9061 tttattttga cgaaatcgcg ggatatctgc cggcgacttc gaatccggct tccagaaacg
     9121 tgatcattcg cttattgaaa caggcgagag ctttcggtgt cgggatgatt ctttcctctc
     9181 agaacccgat cgattttgat tataaggcgc tgtcgaacgc gggcacctgg tttatcggac
     9241 atttacagac ggctcaagat gtaaagcgtt tgatcgacgg tcttgccacc aatgatggag
     9301 gaatcgatca aacggaagcg aaaagacgga tttccgcgtt gaaaaaccgc caatttctgt
     9361 atatgaacgt acatacgccc ggattaaagg ttatgacgac tcgttggaca ttaaattatt
     9421 tagccggtcc gttgacgcga aacaggattc ctttattaat cgaaaagggt ctcagcgagc
     9481 gtgtcgatac cgtaagtccc gtaatgaatg tcaacgaagc tgtcgcagaa ttggcggaca
     9541 ccagtcagaa tcaagaagaa gatcaaggag aagcgaaaat gtccgaggaa aaagccaagg
     9601 aagtccagcc cgaaggcacc aaacccgaaa tcgcagcgaa tataaatgaa tattatctca
     9661 gagcggaaaa aacagcggcg gaagtcggtg caccggccga agaaatcact tatcttccgg
     9721 catggatcgc gcaagccgaa gcgcgtattc ttcaaccgaa atataatctg gattttatcg
     9781 ataaaaaagc ggttttgatt gacgaaccgg acatccgtgg cgtttccatt aattggcggg
     9841 aatatgaaac ggaacctttc gacgaaaaaa ctgttcaaag tcgaccgcaa tatgccgatg
     9901 cgaaatatga acagccgcct gaatggatgt cggattcagg cagtgttcga agtcgcgaga
     9961 gcgattttct cgattatatc tatcgggagg gaatcgtaaa gattcgtgca aacaaaccgt
    10021 tggatgttta tgccgctccg gatgtgactg aagaagattt tattgcgatg tgtaaaggaa
    10081 aagtggctga agcttcacaa aacgaaaaag ataagttggc aaaagcatac gaaaatatcg
    10141 tcgatcgtat ggagacgaaa atacgcaaag cggaagccga tgtagccgac aaaaccgata
    10201 aagtgaagag caagaatata gaaaaatatg gtgcattcgg tgagctggtc atcggaatgt
    10261 ttaccggcag aaaacgatcc gtttctacct ccatcaacaa atatgggcaa tcgaccaatg
    10321 cggataatgc actcgaaaaa gcccaacttg tgttggatga gctgaaagcc gaccttgagc
    10381 agcaaaccga ggaatttaaa aagcaaatag ccgaagtgga agaaaaatgg aataagattg
    10441 ccgaagaaac ggacgaagtg acggtcaccc cgatgaaaaa agatatcttt atcgattatt
    10501 tcggcatctt atggcttcca tactataccg acgagaacgg aaaattcgta aaagctttct
    10561 gagttgccgg cattctcttt cggttcataa aaccattcaa aatccgcttc cttatcaacc
    10621 ggagcggatt tatttttata aggaatgtgt ctcgaatccg tggtgattca aaaaatcggg
    10681 tgattttcaa attacaatat agatatatgg acatcgaaga gacccaacct atcagaccca
    10741 aacgcgggaa tccgtatgtg acattccccg atttttcacc ctccgtgccg gccgaacccg
    10801 gcattgcggt tcccacggaa ttaaacaggg aatcggaggg tcgattcgta ccgcctcctt
    10861 atccgccggt gcctgtttcg ccggaaatgg caaaagcatt atccgccgaa ataaaaagcc
    10921 gcaagatcca atttttgacg cattttaccc gccttgaaaa cctcgcttcc attttaacct
    10981 acgggctgct tccgcccaac caattaaaat cggaaggacg gacacgattt gtgcgcttta
    11041 atccgcctta tctgccgggg tattggcaaa atgcgatcag ctttaatatt tcctttccga
    11101 attatcgctt gttttataat cttcaggaac agcggggata tgattgggtg gttctgcttt
    11161 tcgatattaa tattttattg agccaaccgt tctattactt catctatccg gctgccaatt
    11221 taattcatac acctattttt gcgactgaaa tttcaccgaa attacaaacc tttgaagcat
    11281 ttgaagagtt gtttcaggat acggagaatg ttcgccgagc ttttttgcaa attcccgatt
    11341 gctatcccac ccaccctcaa tcggaagtgt tgacatttca gccggtaagt gtcaacgctc
    11401 ttttggaagt tcatttttac aacgattaca agtttaatca gtggtttatg cagaataccg
    11461 cattggcgat gacgatggat aaaaatatct ggcaggtcag ccttgaattt ttttcaccgc
    11521 gttgcgatta tttaaattgg aaatccaccc aacgataatt ttttcataat ttcttataaa
    11581 gaattctgat acatttccgg ccgattcttt gaaaggtatt ggtataatgg cgggtgtgaa
    11641 aaacaatttc ccggagaata ataaatatca atgacttgtg atctgcaatt tattcaatcc
    11701 attaatcagg gtttcgttga aattctggga aaagaagatt tggatgattt tcgattgttt
    11761 ttgaaagaac catccgaatc ggaaagttgt atgcgatacc tgtcgatgat gcaatgctat
    11821 ttatcggaaa agttcggtta tttcgaatcg gaagggatcg caataagtat cgggaaaaat
    11881 gcctatcgtc ccattaagat tgcatttgaa tcggttcttc attttcagga agtcgacttt
    11941 cggttgcttc ctcatcataa acggattaaa accgctttgg tgcggctgat cgaaagagtt
    12001 tataaaccgg tgggtatccc gatcatgtct ctcgatacgg aagagacaga ttcctgggcg
    12061 attaaaccgg aaggtccgat tcgtatgttt ccggtccaaa tcttaaacgg aattcttcag
    12121 gaaatgctgc tggatgtttc cggcgggcat tttttcccgg ttgaagttca ggaggatgag
    12181 caacggaata tgatcatcct gacttttgcc acaagtccga tcggtcatta aatagccgga
    12241 tttttgtcgg acagtgaaat cattcagttt ggaaggtttt tcggaaataa ggaataagga
    12301 gtctatcatg aaaaaatttt taccgttagt tcttaccatt ttgatcttat tttgcttctt
    12361 ttccgttcag gcggataaga atgttctgaa tgttttgacc tacaactctt ttgccgtcag
    12421 tgaagatgtt attaaagatt ttgaagctga aaacaatgtg aaagttcagt ttattgaggg
    12481 cggagatacg ccttccattg taaatcgggc aattctcacg aagaattcgc cgattgccga
    12541 tgtcattgtc gggatgaacg atgttttact ggcacgggtc cttaaaaatg aaattcttga
    12601 accgtacgaa tcgccggaat tgaagaatat tcccgatgaa tttaaatcgg atcccgcctt
    12661 cggagcgtta cccttcgatt acggacatgt ttgtattaat tacgataccg cttatttcac
    12721 agatcatcaa ttggcggtcc cttcctctct cgacgatttg atttcggaac cgtacaaagg
    12781 gcttttggtc gttgaaaatc cgacttcttc gggtccggga caggcttttt tcctgaccac
    12841 cgtcgccgag tacggcgaag acggatttgt cgattattgg aataaactgt tggcaaacga
    12901 tgtgcaaatc gataatgatt ggtcttcggc ttattacacc agttttacgg ccggaggcgg
    12961 aaacggtccg cgaccgatgg tcgtttcata tgataccagc ccggctgccg ttccttttta
    13021 ttcggagact ccgatggaga cggctccgac ggctgcggtt ctggcggatg gtacgtgcta
    13081 tcggacgatc gaatttctgg gaatattaaa gggaaccaag aatcgtgaaa tcgccgagaa
    13141 atttgtggat gccatgctgg ggaaacgttg gcaagaggat ttacccggac agatgtttgt
    13201 gtatcctgtc aaccgagaag cggttttgcc tgaggtcttt gcaaaattcg cgatccaacc
    13261 cgaaaatccg gtttcattgg attcggcatt gatcgaagaa aaatacgaag attggattca
    13321 aacctggaac gacgaagtat tggtgaatta ctaaacggaa agaagagaaa ttataaatgt
    13381 caatagtctg cagcggttcg gtagcatatg attatctgat gcgtttcccg ggatattttc
    13441 gagaccaaat tctcccggat cacttggatt cgatcagcct gtcttttttg gtcgatgaca
    13501 tggtgcggct tcaaggcggt gtggctccga atgtctgcta cagcctggct ttgttggggg
    13561 aaaagccgat cttgtttgca gcggtcggcg aagactttga tcagtatcga aaatggctgg
    13621 aagagcgggg cgttgattgc cgctttgcac gggttatacc gggtaagttt accgcttctt
    13681 tcttttctaa tacggatttg gcaaacgctc aaattgcttc attttatacg ggggcaatga
    13741 agaattccgc cgatctgaaa gtatccgagc tgaaagatga gaaggttgat ttgtttctta
    13801 tttcaccatc cgatccggat gccatgatcg ggtatgcccg gcaatgcaaa gaacttggga
    13861 ttccgttcgg attcgacccg tctcagcaaa ttgttcgcat gaacagagag atgattcgcg
    13921 aggggttgga cggcgcaaaa ttcttgtttt gcaatgaata tgaatttgag cttctgaaaa
    13981 aacacagcga atggagcgaa gaagaagttc ttgatagagt ggattgtgcc gtgattacgt
    14041 tcggagaaaa aggatcgcgt atttatgccg acggatcggt tacgacggtg cccgtttatc
    14101 cgcccgaacg aattgtcgac ccgaccggag tgggcgatgc ttatcgatcc ggtttcttga
    14161 ccggttatta taaagggctg gattgggaat tatgcgggaa gatgggagct ctggcgtcca
    14221 cttatgtttt ggaacaaaag ggaacacaat ctcaccgtta tgatcgggaa acttttatcg
    14281 aacgttttcg aaaattatgg gacgacggcg gacgattgga ttgcctctta taaaccgggc
    14341 ttttcgttgg aaaatggttt ttacttagtg caaagacaga aaaaaaagag aatagagcat
    14401 tgagcgaaat gatctttgga gaatgatgcg agaccgagtg accggttacc gaggttaccc
    14461 ggtctttttt ttattcgaat ccgattttcc gaaacgttgt ttccattcgt aaaggatgtt
    14521 aattgcttca atcggcgtca tgctgttgac atccaatcga tccaaggtat cgataatcgg
    14581 atttgtctcc ggaaataaag tcagctgata agacggtgtc tcccgaattt ccatctgctt
    14641 gaccgaatcc gattccaatt gtttcaaaat ttcgtttgcc ctggaaatga ccggttttgg
    14701 caagccggcg atttgagcga catgaatacc gtaagaacga tctgcggcac cgggaatgat
    14761 cttatgtaga aatacgactt ctcccgcatt ttcggtgacg gccacattgt aattttttac
    14821 gcccggcaag gaattcgcaa gacttgtcaa ttcatgataa tgggtagcga ataatgttct
    14881 ggatttcaga ccgggggcgt tatgaatgta ctcgataatc gcccaagcga tggaaaggcc
    14941 gtcatacgtg ctggtccccc gtccgatttc atcaaggatc agcaagctgc gatcggttcc
    15001 gttatttaat atgttggctg cttcgaccat ttcaaccata aatgtcgata atccggcgtg
    15061 aatttcatcc tgggcaccga ttcgggtgaa tatccgatcg accaagccga tttttgcgga
    15121 agatgcgggg acaaaagaac ccatctgagc catcagaacg atgattgcgg tttgacgtaa
    15181 aaaagtgctt ttcccgctca tgttcggacc ggtgatgacg tgaataaagg aatttcctcc
    15241 caattttatg tcattgggga taaaacgggt tccttgggga agtgtctgtt cgacgaccgg
    15301 atgtcttcct tcgttaattt cgatctccag gctgttatta atttccggtt taacgtactt
    15361 attgcgaacg gcggattccg ctaaagcagc caaaacgtcc aatgtggcga tccctcttgc
    15421 ggtggtcaga atttccgttg catattgact gatttcggca cagatttgat agaacaaccg
    15481 attttcgatt tcgcgaatgc gctcttccgc gttcaacacg agcgcttcat attctttcat
    15541 ttcgggtgta atgaaacgtt cggcattcac gagtgtttgc ttccgaatat attcgggtgg
    15601 tgcctgatcc gcgctcccgc gagagatttc gatataatat ccgaaaactt tgttatagct
    15661 gactttcagc gttttgatac cggtccgttc ccgttcgctg ctttccagat tagccatcca
    15721 ttcccgagca tggccggatt cctgatgaat tcgatccaat tcttccgaat aacctgcgcg
    15781 aatcacaccg gtgttcatca gagtggcagg cggatcatcc gcgacagccg attccaatat
    15841 ttgcttaacg acttccaacg ggtatatttt ctgaataatc ggcgtcagag ccggcaaaat
    15901 cggttcgata atctcgatga tttccggaag tttgcgaatt gaactgcgca gagcggtaag
    15961 atcacgcggg attgccattc cacccgaaat tcggttgatc aaacgttcca tatcattcat
    16021 tcccttcagg ctttcccgca cttccgctct gatcatcccg tcattaaaga atgattccac
    16081 cgcatccaaa cgattttgaa tcccattttg attggtgagc ggtttactga cccattgccg
    16141 aataaccctt ttccccatcg gagtgacggt gcaatcgatt acacccaaca gcgaaccttt
    16201 gagctcgtta ttacgcaggg attcggttaa ctccaagttc ctgcgtgtag atgaatcaag
    16261 caccatgaaa tcatccaaag aatagaaaga aattgaagaa agcagtttta tgcttgaagg
    16321 ttgattgtct tgtaaatatt gcaataagca gccggcggct ctgaccggta tactattttc
    16381 tttcagaccg tatccgtcca gcgtggcgac ttgaaaatga tcccggagca gttcggtaca
    16441 gcgtaacggt tcgaagttat aatccggcac gcgagtaatg tgcagatttt cggaaagcgg
    16501 caggtcaaaa gactgtgcga ccaatatttc tgccggatta agacgggtta tttccgattg
    16561 taaatcagcg gtaacatccg cactttgaat ctgagtgacg gcaaattcac cggtggtgat
    16621 atccgcatat gcaatcccaa tcttgtcatt ttcgacacag gctgccatca gataattatt
    16681 ggaatcgctt tttaataacc cgggttcaat taaggtaccc ggagtcacca cacgcaccac
    16741 ttcccgttca aagagacctt tcgtgggctg atccccgatt tgctcacaaa ttgcgacatg
    16801 atagccctta tcgatcaatc gagacagata gttctcgacg gcatgataag ggatgccggc
    16861 catcggcacc cgtacaccct ttgcaacatt ccgagaagtc agcaccaaat ccagttcacg
    16921 agaagttgtt tcggcgtctt catcgaaggt ttcataaaaa tctcccaatc gaaagaaaac
    16981 gattgtatcg gggtattgtt ttttaatttc aaggtattgt tgtcgaatgg gagtaaattc
    17041 ttcgctcatt taattgtttt tccgtatcgt aaaagataag ctgcaatgcc gaaagcagtg
    17101 gcgacattca gagattcttt ttctccctgc atgggaagaa atacactgaa atcagctttt
    17161 tccaagacaa cgggatcgat tccgttaatt tcatttccga taaacagagc aacgggttgt
    17221 ccggataaaa ttttaccaat attttggaat aaattaatcg aacgcttccc gccttccagg
    17281 cataaaatca ggaaaccttc cgattttaat tgttcaacaa cttccagaat attccatgag
    17341 tgtttccatc ggatcgcatt ttctgctccc aaggatgtct ttaagacctt cggattttcg
    17401 ggagtagggg tgataccgca aagaaaaatt ttccgaatac ccgctccgtc agcggttctg
    17461 agaatggctc cgacattgag agcacttcga atattatcta aaaccgcgac cacagtcggc
    17521 aacccggatt catcttgctt tcgttggggt atcaagttcg aatcgtaacc ctcatctatc
    17581 ggaatcgtcc cgctaccgca tcgcggacag cggatcgaaa tattctctga aagcggaatc
    17641 gtaaagcgta aagtacaatc gggattaata cagaggcgtg tttggtgttt catcgctttg
    17701 atttataaag tcaagacaaa atttcattcc gttttcgatc attcttattc ggatcgttga
    17761 tcgaacaaac aaataaaggt cataaagatt aaaaggcaga ggatccaaag agggttcctc
    17821 cgccttttta caattctctt ccctataaga gatccgttac ggctttggat cccaaattga
    17881 ttcggatggc tcgacgttct tttgccaaca gcgcttcaaa atcccccaaa gcctgtgctt
    17941 tttccaagat tgaaaaagta atcccttcat tgggaatttc gatatcttgc ggcgcgtccg
    18001 cgatcagttg gataaacaga ccgttattcg cacccccttt atgtaattgt ccggtggagt
    18061 gctgaaaacg gggaccgaaa ccgagagtgg tggcacattg agtttctttt aagatccatg
    18121 cccgaacatt ttgcaaatct gccgccatct tttcatttct gggtaaataa gcattgattg
    18181 caatgtaatc tttccctttt attgcctgcc gaatgaaagc cttgatcaga tgcttgatat
    18241 catccgcttg tttcaaatct ggtatatctg tattgaaata aacgctgagg ccgtcttttt
    18301 cccagcaagc gggaaaatct gtcagttgcc ctttttcttt atattccgta atcttggcga
    18361 ctgtacgatt tttactgtcc tgcacattcg gttgatcaaa tgcatttaca cccagcagcg
    18421 agcaagcaac cgcaacggcg atttcccaac gataaaactc ggcaaataat tcgttcaaat
    18481 cattcaattt tatcgtgaga accggttgac cggctgtgat caattcattg acaaagtctg
    18541 tttgtgtacc ggatgtctcc acgtaaacaa atgctcgatc ttccgcatat tcagaaggag
    18601 ccagtttcgg ttcgatatcg atcggaacaa ttcctttgcc cattttcccg gtgctttcgg
    18661 cgatcagctg ttccaaccag gagccgaaag ctgaaaattc cggatcggtc aaaatagtga
    18721 atttatctct gctgttcagg gtagccgtgc ccatataaac gcccagagca gcgccctcat
    18781 tacatccgac agaattggaa ggagcgcaaa tttttgccat gcgggtagcc tgttcgataa
    18841 ttgtttccgg attgattccc atcaaaacag ccggaaaaac tccgaatgcg gataatgccg
    18901 agaatcgtcc tccgatggag ggatccgcca aaatgatatt ccgaaatccc atttctttgg
    18961 ctttgcgttc caactgagta ccgggatcgg taatggcaat gaaatgggat ccggcctgat
    19021 cagacagttc tgttttggct ctttcgaaga aataatccaa aaaagcctga acttctgctg
    19081 tccctcccga tttgctggaa acgatataga ccgtttcttt gggaggatga gatgcgtcgg
    19141 ccgccaagac ttgaaccgga tcggtggaat cgattatttc caatgccatc ccatcgttat
    19201 ttttgaaggt taatgccatt acttcgggag caagcgaaga tcctcccatc cccatcagca
    19261 aaatcttatg gatgccgtcc tttttcaagc cggcagcgat tgatttatat tcgtcgattg
    19321 atttggcaat gttttgatgc gcatccaacc atcccaatcg attccgaatt tcgggatagg
    19381 cttgtgtatc gaatgtccaa acggtggggt cttttgcgaa tattcgctgg ataatcgaat
    19441 cttcttcgag tttcttaaaa ttctcaaaga ccggagtgta aaggcatccc aatgattttt
    19501 tcacccgatc cgactttttg tctaattctt ttaacattga ttcaaaggca cttttataag
    19561 attccatgat ttcctcctga acacgttccg taagcgattc gaattgaata ccgagctttt
    19621 caaattcttc aataatttgg ttatcttcct catgcggtac tcgaagggat ggtatgatct
    19681 gtccatgatc gataacggcg tccattaact tcggatgaac ggataggatc gtattttctg
    19741 cgattaacga atccgtataa taagtgtctt tgaatgaagg ctccttatgg cagagagatg
    19801 accataaaag atgttgtatt tcagcgcctt cagcaattag attttgcatt ctgccgcttt
    19861 gattggtttc caagaaaaga ttgaagattc ggcggcaaat gatcaatgcc gctttcattt
    19921 tcaatcgacg ggtagcggtt gacttatccg gatcgacagg atcgacagcc tgatccaaaa
    19981 atttgttgac gatatgatcg atttccgcaa cgtcgacaga agctgtggaa taaattttat
    20041 cgataggttt cccttctttt aatcgcgttt ccaaaccgga aagataggcg tcaacggcac
    20101 ccgaatagct tttttcggaa acaatcattg taaaattgat attgatccct tccgttacca
    20161 actgttttgc ggcttccaaa ccttcttcgg tggccggaat gccgatcaaa atattaggcc
    20221 gattcaccgc agtccaaatc cgtttcgttt cggacacggt tttttcgata ttatgtgaca
    20281 attcaggatc cagggttaga gtgacatatc cttcttttcc gctgctgctg atataaagat
    20341 ccttaaaaat ccccgcagcg gaccgaacat catcaaccaa gagtttatcg aagattttgc
    20401 ggcaattcca gccggcaagc atcatgggaa ccaaagccga cgcatagtca tcgctgttca
    20461 agattgcgct gcacatatta ccggaattca ttattaaacc gcttacctga cctttttcga
    20521 tcattttgac caattcgccg tttttcagtt ggcagcgatt agcctgatcc aaccagacag
    20581 attgtcctat atcccgtaat gcttcgattc ggttgatcag gtaaaggtct ccggcttcga
    20641 tttgtttgat tttattgaac cgcctgattt gattcggctt attattgaaa gcggcaccga
    20701 gaaatgcttt aaccaactcg tttgccaatt cgggtccgat gattctggcg ccgagacaca
    20761 agacattcat tccgtcatgt tccactccct gatgagcaga ataggtatca tggcaaactg
    20821 atgcatagat ccccgggatt ttattggcgg cgatacaaac acctactccc gatccgcaga
    20881 gaaaaatgcc ggcgtctgct ttccccgatt taattttttc ggctcccagt tgaacgaaat
    20941 cggggaaatc cgaactttct ttggaaaatg ctcccacatc gatcacttta cagccggctt
    21001 cctcgactgt ttcaataata ttttgtttca gctcaaatcc ggcatgatcg ctggcaatta
    21061 ctactgtttt attcaaagga atctcccatt atctgacaaa aaaacctatc tttcaatttt
    21121 gttacggatt caaaatcact ttcaatgcgt tcactttttg ataataaaga ttatcaaacc
    21181 attccggacc ctctgataac ggagctgtag cgctgataat cggattcaca ttaaaagaac
    21241 ctcgggcaat catattcaaa catgccggat attctccgga agatgcacag gatccttgca
    21301 atcggatttg tcgggtaaca acggattgaa gcggaaagtt cgttttctgc gaaatatttc
    21361 ctacaagggt gactgaacct cctttatgca cactattgac ggccaggttg aacgttgcat
    21421 cagcaccgac cacttcaaaa gcagcatccg ttccttcccc tccggataac atgcgaattt
    21481 tctcgagtgc gttctcatcg gaaattaccg tatcatccgc gcccatctgt tttgccaatt
    21541 ccaatcgttt ggaatcaacg tcaacggcga tgatttttcc gtagccatac gctcgtaaag
    21601 cttgaatcac caataaaccg accattcccg ttccgaccac cgttaccgat gcatcgagct
    21661 gatgcgggct tagatctgct gcatgcacgg cgacagaaag cggttctacg agggcggctt
    21721 gattaaaatc gacttcatct ggtaacgcat agaggatatg ctgaggaaca gcaatgtatt
    21781 cggcgaacgc accgtcccga cgatattcgt cacacgacac gcctaaaaca cgacgatttt
    21841 cgcacagatt aatcagacct tcccggcagt aggaacaatg accgcaataa atcgtggaat
    21901 caaaggtaac gcgatcccct tttttccacc caaggacttt tgctccggtt tcaacaatga
    21961 ttccgcttgc ttcatgcccc ataatcaccg gcggctgccg cctgcctgat gaaccgtcat
    22021 atccgtgaat atcggaaccg caaattccgc aggcttttac ctgtatcaac acttcatctt
    22081 ctgcgatgat cggcaccggt acatcttgaa tttccatttt tttataatcg gtcagaacta
    22141 aggctttcat ttttttcttc ttttcctttc aacggtgtga ataagggacg taacttaagc
    22201 gaccgtcaaa atcagatcca tcttcggcgg agagcgggaa ctttatcggc cggtgatccc
    22261 gttgggaacg ataaacggcg gtgaatatct caaccgtttt tcgaccttca tgtccgtcaa
    22321 cggagggcgg tcgattatga atgactgcgt ccagaaaatc ggcgatttgg atctgatgga
    22381 aataagtcat cggatcgatc ccttcggatt tgatttgatc ctctttcttc cagacttccg
    22441 gcatgttctc ttcccccggg acagtccaaa tatcattaaa agccggttcc acttttgagg
    22501 tggttacgcc tgaaataaaa gaagaacctc cctctacttg tgctcccacg gaagcgccgt
    22561 ttgaaccatg gatgtgaatt ctgccccata agcccggttt ttgagaattc gaaacgagta
    22621 tttgaccaat tccgccgttt ttgaatctaa ttacggctaa cgccgtatct tccacttcga
    22681 tttccgggtg gttgaaatta tcccaatagc cgaataattc atcgatgtca cccatcatcc
    22741 attgcaataa atcaatctga tgaggggttt gattgaccat tacaccgcca ccttcttcgc
    22801 gccattttcc ccgccaacca tccatctggt aatattcggg gctgcgccaa cccataactt
    22861 ctattgtccc gatgattggt ttgccgattt ttccgtctcg tatggcttga atcatgcgta
    22921 aaaccggcca ataaaaacga cgctggctga taactcccaa tttcacaccg gctttttcgc
    22981 atgcggtaat tgcccgatca cagtcccgaa gatcggaggc caacggtttt tcacagatca
    23041 cgttcgcacc cgcttccgcc gcttcgcagg tcggttcggc atgcaaagga tgcggagtac
    23101 agaccgaaac aacttggacc ccattctcac gaatcatttt ttttatatcg gaatatgccc
    23161 gaacattgta gtctttcgca aactgattcg cattcttttg tgagacatcg cataccgcac
    23221 agagctcgga atttggaagc gttttataag caagcgcatg agtggctccc acttttccgc
    23281 atccgatcac agccgttcga attttatcca tttacattcc cttccgttat ccaaattaag
    23341 tttcacctgc ataaattatt gagaaatcat acgggcttct tttatcattt gggttttcaa
    23401 atcccacaac ttttgtgaaa gatcaacata ataagtatgc ggattatcca accgacgaat
    23461 ttcttcccat aattcatcga catttttctg acaatttctt cgaatctgtt ggagcgtcgg
    23521 taacttataa accaatttcc ctttctcgta aatcgggacc agaagtttct ttgtatgaaa
    23581 gtttgtaatc actttgcgtt tggtcggatc taacggatca aaaatttcaa tcgggacgtt
    23641 atcttcgatc acttcatctt ccaatgtgat gacatccgcc aaaggatggt tatcgccgat
    23701 ttcgtataag cgccatatct ctttaaagga agggtttgta atcttttcaa aagtatcgga
    23761 aattttaatt tttggaatca agtttccatt ttcgtcataa atacccacca gcttatagac
    23821 accgccgaaa atcgcttcgc tgctggcggt tatcaatctt tctccgacac cataaacatc
    23881 cacacgggca ccctgaacat ttaatgatcg gataatatat tcgtccagcg aattgctggc
    23941 aaatatttta caatctttca gtccctcatc atccagcatc tttctggctt ttttggacaa
    24001 ataagcgatg tcaccgctgt ccaagcgaat tccgaccaac cttttgccca tcggctcgag
    24061 tacttcatgt gccacacgaa tggcattcgg aacgcccgat ttcagcgtat tgtaggtgtc
    24121 gaccaaaagg ttgcagttat ccggatagct tttcgcataa gctttaaaag cctcatattc
    24181 tgaatcgaaa gacaaaatcc aggaatgtgc catcgttccg cttgccggaa cgccgaggtg
    24241 aatatccgcc cagttatttg ctgagcctgc cacgccggcg atataagcgg cacgcgcacc
    24301 gtaattggcg gcatcatatc cctgggctcg gcgagtgccg aattcaagaa cagttttccc
    24361 cttggcagtt cgaacaatcc gattcgtttt tgtcgcgatc aaggattgat gattgatcgt
    24421 taccagtaac attgtttcga ttaattgagc ctcgattatc ggaccccgaa cccggactaa
    24481 aggctcgttg gggaaaacgg gcgtaccttc aggtatcgcc cacacatcgc aatgaaattc
    24541 gaaatgacgc aaataatcaa taaaatccgg atcaaagagc tttttattcg tcaaatatgc
    24601 cagctcatct tcggtaaaat gcaaattttc aatatattgg acgacttgtt ccaagccgca
    24661 tataatggca aagcctccgt catccggtat tcgccggaaa aacaaatcga aaataacaat
    24721 tttatctttc atcccttgct tgaaataacc gttgcccatt gtcatttcat aaaagtcaaa
    24781 aagcatactc agatttcttg aatcgtattt ttcacgaaga ttatgtgctg tcattattca
    24841 acctttattc catttaattt catatgttcc cagaaaaacc gattcagatt atccgcctcg
    24901 tggccttccg aatcataggt atcgaccaaa tccttgtgaa cgattatgtc cggtattctt
    24961 gagatccgct ttttatgata ttcgtattcc atgtgttttt gagtaatcaa acaaaaattg
    25021 aagacacaaa ggtcggtaca acacccgacc acatgaattt cctcataacg acgtaaatcc
    25081 agcgatttgt tttcttgaga tgcttcctgt atttcaaaaa atccaccggt cgtaaatttc
    25141 ggaaggataa aatcacaata aggtttaatt tcatcgacca cttccgattc atcactgccg
    25201 gccaaacaat ggctcggaaa cgattccatt tcaatatcct gcggatcatg cgtatccgaa
    25261 agagcaagaa tagtccaacc tttttctttt gattttttac aataatcggc tatcggatcg
    25321 atcagatttg ccgttcgagg actaaaaagg tttccttttt ttgcaaatcc gttattcata
    25381 tccatgatca ccagcaaatt ttgcatatct atcctccctg tccctataaa ataaccgcag
    25441 catttcaaat gtctattata aataggttat cgaacttgat ctcaacaatt tctatcttcg
    25501 gcaaaatcat cttttctttt ttccctctcg ttcattcata aatattgaat cgacagagtt
    25561 tctattgaat ttaagatgat tattcctttc gtacgattct atgagaaaaa gtttgtttag
    25621 atgagtgatt ttcatgacga atcgtttccg aatgccgaat ttgatctatg aaaccatcca
    25681 ttccgattat aatctgaaaa agatttgata ttttgaaaga taaaaattat gctgcaagat
    25741 attcgagtac gtcaacggga ttatctgatc gagttagctc gtgttctgac tcaagaaatg
    25801 gatctggatg ttcttctttg gaagatttta cggattacat tgaatttatt gaatggggaa
    25861 gcggggttca tcgctcttta tgattcgaaa gaaaaatggc atgtcagggc tatttcgggt
    25921 gtattggaac cgacggcaag atatatcgag acttatttaa aaaatttttc ggaacgtgaa
    25981 gaaagttttg atcaaaatgc attgatcgag atcaatctgt tgattcagcg aatttgtcag
    26041 atgccggaac tgggctttgc ggacggtgtc ggtcttccgt tggtggaaca cggtaatttt
    26101 ctgggaatta ttgtgatctt tcgcaaattc cataccggtt ttacgataaa tgatttagcg
    26161 atgcttaaaa tctttgccga tcaagcggcg atcgcgattc ataacgcgga tctttacggg
    26221 gaaaatattc gcgagaagaa tcggataaac gccttatttg acgcggttcc ggacggtatt
    26281 ttcgtcctcg atctcagcca tcatattata cgggttaacc cggcaatgac caaattgctt
    26341 catgtcaaag aggaaacctt attgggggaa catcacgatg aggtaattgt tcttgagaat
    26401 atcacagcgg gaatggaatt atcagccggg gaagcgggcg gatggccgtt ttcaaaagaa
    26461 tccaagttat atgtggaagg aaatttggct tacggtccgt tgaaagaaaa accgctgccg
    26521 gtcggaatcg catattctcc cgtgatgaat gatgaaaata ccatgatcaa tattatcggt
    26581 ctggtacgag atatttccaa atttcgtgaa gcggatgatt tgaagaacac attcatttcc
    26641 acgatgagtc acgagttaaa aacccccgtg gctttgatca aaggatatgc ctcaacaatg
    26701 cagttggatg atgccaaatg ggataaagat ctcattaagg aatcttttgc gattatcgaa
    26761 gaagaagccg acagactcgc caacatgatc aacagcctgt tagatgcttc ccgactttat
    26821 gccggcgcat taacactcaa ccgatccgaa ataaatatag ccgaactggc acggaatatt
    26881 gctaaaagga tgcagactca aaccgataaa catactatcc gctgcgaatt tccggcagat
    26941 ttgccgatta tttatgggga tgcggatcgg atcgaacagg ttctgatgaa tctgatttcc
    27001 aacgcgatca aatacagccc gggcggagaa attctgataa gcggagaact acagaatgac
    27061 tttgtgaaaa tatcggtaaa agatgaagga caagggttac aaagcgaaga tcttcaacat
    27121 ctttttgaac gtttctaccg gtccaaacgt tccgccaatt tggcaaaagg tgtcggtttg
    27181 ggattatatt tgagcaacgg tatcgttgaa ggtcatggcg gaaaaatgtg ggccgaaaac
    27241 aggacagatc gcagcggagc catattcagc tttacgcttc cgctcaatcc ggatgatcat
    27301 cccgctgcat taaaatttcg tgatatcggc gattacaaaa atcacagctt atagccggaa
    27361 gaatccttat tttgtgccga aggaatctgc cgtgtagacg caacgaaaac cccgattaaa
    27421 gccggctgat ttttcactat gccactgaaa agcggttatt tccaatgttt gaggataatc
    27481 act
//
LOCUS       DAOSMM010000024        26742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1809_length_26742_cov_17.919699, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000024 DAOSMM010000000
VERSION     DAOSMM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 26742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26742
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1809_length_26742_cov_17.919699"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK898_05220"
     CDS             complement(<1..523)
                     /locus_tag="PK898_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOU19470.1"
                     /translation="MDIKNNLFNSPAKKLFLLAILLLIALILSSLAGRFPLPGLQSPR
                     ALLESSLNRTILIHIRLPRVIISLFMGMALATAGLIFQTILNNPMVEPGFLGVSQGAS
                     FGAALAIVLLSGKQWQIQILAITFGIAGLTFSIFLGSRFHFGHWVIRLVLAGIAVSAL
                     FSSGLGILKYLADR"
     gene            complement(514..1296)
                     /locus_tag="PK898_05225"
     CDS             complement(514..1296)
                     /locus_tag="PK898_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19471.1"
                     /translation="MSPKETVLELRDIQFSYDKSDSFSLSSINIKMHSGQIVVILGPN
                     GSGKTTILKIANGRLQPDSGGVYLEGKPLQSFSRREIGQRIGFVPQAEKISFEYTNLE
                     YVTMGRTPWLNPLERPSEKDIEISREAIREVGMGKFMDLPVTKLSGGQSQLILLARAL
                     AQETEILLLDEATSQLDLANKRHFLDIVRNLAAAGKTIFLSTHEPDVAAALADKAVLM
                     KNGQVLIAGPMESVFTKENLQHIYDTPIEIMQLDGRKVVLWT"
     gene            complement(1293..2276)
                     /locus_tag="PK898_05230"
     CDS             complement(1293..2276)
                     /locus_tag="PK898_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU19472.1"
                     /translation="MMQRNKRIKLIALTGILILCLFLSIFAGRYPELGFLSPATLKED
                     TTARNIFFNLRIPRIILGLLLGMALGSSGLFFQTVFNNPLVDSGMLGVTQGASFGAAI
                     GIVFFHSNPWIVQLLAIGLGMSGLFLSVVSARKIHYGGWILRLILSGSVISALFGAGL
                     SLLKVLADSRNQLHAIEFWLMGSLGFTIWQGLPLVIVITCVCLFILWKYRWRINLLAL
                     DSEVASSLGVRIRMEQMLLMTAAVFAVSAATSLSGIIGWVGLIVPHLSRRLFGSDTQY
                     SLPGSMLIGAILILACDNGARILSPSEIPLGIITSFVGVIIFILLMGGKKK"
     gene            2411..2914
                     /locus_tag="PK898_05235"
     CDS             2411..2914
                     /locus_tag="PK898_05235"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOU19473.1"
                     /translation="MADKKEILIITGSRGAGKTAYCRNEVQKARENSLIVRGILSPAM
                     FLNHKKIAFYAQNLKNDEKRLAGSLASYANLGKQFHDWTLDPDVIRWVNEFLSEKEPC
                     DLLVIDELGPLEFSDHEGYLAAFDCLKNQNYRKALVVIRPECIDAFQSFGLPFSIIEI
                     EKADADS"
     gene            3071..3143
                     /locus_tag="PK898_05240"
     tRNA            3071..3143
                     /locus_tag="PK898_05240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3104..3106,aa:Gly,seq:gcc)
     gene            3166..3237
                     /locus_tag="PK898_05245"
     tRNA            3166..3237
                     /locus_tag="PK898_05245"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3199..3201,aa:Cys,seq:gca)
     gene            complement(3349..4701)
                     /locus_tag="PK898_05250"
     CDS             complement(3349..4701)
                     /locus_tag="PK898_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19474.1"
                     /translation="MKKKLLSIILSLFIFLAIPNFSGLAQSSVTQYIVNLYFFRGEGC
                     PHCAQEEVFLQQMTAKYGDQLVIHEYEVWYHPENVTLLEKFAEKYGFEPSGVPVTFIG
                     NQYWVGFNDIKQQEIETAISSGIQNGVVDTQDIVDGKTELIPPEKALSAKITIPLIGQ
                     IDLAQETLFVSTVIIGLVDGVNPCSLWVLTMLLAMLIHTNSRKKMTIIGLVFIIVAAL
                     IYALFITGVFTLLTYASYLKWIQIVVAVITLVLGLINLKDYFFFKEGVSLTIADEKKP
                     GIYQKMRNVVNNSENLWAMIGATIVLALGVSLVEFSCTAAFPVVWSNLLVANHASKAT
                     FIALLILYLFLYQLDELVIFTITVISMKSSRMQETHGQILKLFSGCLMVSLSLVMLID
                     PALMNNLSSSLLVFGVAILITVIIYLVTDKILPRYGIRIGHTRKSDPVKGKPVRKKSK
                     "
     gene            complement(4866..5435)
                     /locus_tag="PK898_05255"
     CDS             complement(4866..5435)
                     /locus_tag="PK898_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HOU19475.1"
                     /translation="MNIRWRYFIPESSANGPGKRCVIWFQGCSIGCEGCCNPDLQDPK
                     GGRSDPVETVFEKIRSVAKNVDGITISGGEPFQQPEALCELVRMIKAETTLSVFVFSG
                     YQKEKILSDPKRNRCLPWIDAILYGPYIHSNPPALERFCASGNQGLWLLSDRFQAEDF
                     SDLPVLEFLIGEKGELIQSGLYGINFIEK"
     gene            complement(5435..5656)
                     /locus_tag="PK898_05260"
     CDS             complement(5435..5656)
                     /locus_tag="PK898_05260"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HOU19476.1"
                     /translation="MAQLEEIEILIDEKGKVTFQVRGIPGKACLDTTKQLEEALGNEV
                     IKREYTDEYYSDKPVENRSRLRLKTKREL"
     gene            complement(5665..7233)
                     /locus_tag="PK898_05265"
     CDS             complement(5665..7233)
                     /locus_tag="PK898_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOU19477.1"
                     /translation="MKTLAIDFGNTNTVLAFRESSSREVQCIDLPGISEPGSVLIPSM
                     IHYGDGQTLIGSQVIREEKENSYNTFRWMKRYIGMNSPYHIRVNGEKISAKQAAEEFL
                     SNLIRETEERFSVHADELVFSVPVESFEYYANWLISCFTIDGFRKVRIVDEAVSAAAG
                     YGLKMHPGEPALFFDFGGSTMQATVVLLHEPNGREGFLPESFQVIGKSGCSIGGSTVD
                     RWIFEEALMQNRRDFSDPVIHRDGRIILAACEQLKERLSFFEEADLSLILTDHRKFTL
                     RQSREDLETLLRKRGLFQSVDAVVRDALLQAADHGIDASAITAVIPVGGGSMIPAIRS
                     ELGNVLRKALVLNGDPMTAVAKGAINLIDGFGVLDFVRHQYAVRIRDGKTGEFRFHTI
                     IQPGTPYPSDEPVTTLKIKASRQNQSLFGLAIYEISQPAFRPEAQTEIFFDEEGAIRL
                     FSRVDTEKEGEDTFWLNEHAPLFLHADPPAEKGIARFKAEFKVDRNKMLLITVYDLSS
                     GLCLFEDYPVIRLN"
     gene            7370..8512
                     /gene="galK"
                     /locus_tag="PK898_05270"
     CDS             7370..8512
                     /gene="galK"
                     /locus_tag="PK898_05270"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOU19478.1"
                     /translation="MLEKIINAFKNRFGADPDFVVRSPGRINLIGEHTDYNEGFVFPM
                     AIKYAQWLAIKANKTDHVSLYSADIDKDVVFSVTDFSQKITGWACYPQGIAWALHDNG
                     YPLKGWDGLLLGDLPLGAGLSSSAALDLVTARAFSLTGGFQWDPIQMSVISKQSENQW
                     IGLNNGIMDQLISATGEKGKAILIDCRSLDIQPHALPTDTIIVVMNTMVKHALVESGY
                     NDRFRQCMEGAKAFGKKFLRDVTMEEFNDGADQLDEVIRRRCRHVISENARTLEAAGC
                     MERNDPVRLGQLMNESHASLRDDFEVSCEELDIMAEEGQKQPGCYGARMTGGGFGGSA
                     IALVKTEYAELFMKNIASIYEAKTGIKPNIFATDAERGTSVEYTKD"
     gene            complement(8584..9606)
                     /locus_tag="PK898_05275"
     CDS             complement(8584..9606)
                     /locus_tag="PK898_05275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOU19479.1"
                     /translation="MNKRIHILGGAAIILGAILAGLVLSIFLSNNHRVRASQTTDLPT
                     VTLISEMIPTHTTVLPTETMMPSPVVPSAIPTVQPTALSAADWKNWPILPERVSTKMI
                     DVYRAGQENGNKANRFSKVGDSNSIMPSFLGCFDYGENGYKLGKYAALEETIKQFQWS
                     FSRESRATANGITAMQLDTYHWYEDDVCWPYESATSCEYRLWQPSISFIALGTNDVYM
                     PLAEFDKHMRSLVQKSIDRYVVPILVTKADNLEGDGSFNQAIAQIALDYEVPLWNLWR
                     AMDPLPGHGLRENDVHPTFNNTSLCDFSGDDLQTYGWTVRNLTGLQALDRVWHLLNQG
                     MTSSTQ"
     gene            complement(9621..10073)
                     /locus_tag="PK898_05280"
     CDS             complement(9621..10073)
                     /locus_tag="PK898_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOU19480.1"
                     /translation="MFLTEFFGNPAVVSGLAAMMLAQLLKLLLNYALNKKWDWVLLIE
                     SGGMPSSHSAMVTAIAVSIGLWEGFDTAAFAAAFGLMVIVTYDSANVRWQSGLHAQKI
                     NQLLRDVFSGQPINDQLLKEVIGHTPRQVYIGVILGILVALAVDFLWV"
     gene            complement(10060..10290)
                     /gene="xseB"
                     /locus_tag="PK898_05285"
     CDS             complement(10060..10290)
                     /gene="xseB"
                     /locus_tag="PK898_05285"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOU19481.1"
                     /translation="MENSSEKLTFKEAFEQLQKIVEQLESETVELEQAMELYKAGMQL
                     SKYCSSLLEQAELKIRTIGQTTVNGDKDSVSD"
     gene            complement(10306..11517)
                     /gene="xseA"
                     /locus_tag="PK898_05290"
     CDS             complement(10306..11517)
                     /gene="xseA"
                     /locus_tag="PK898_05290"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU19482.1"
                     /translation="MQNFDFFSFADFALSVSEVNQQVKDLLESDPVLEDISVKGEISN
                     LSRPVSGHVYFTLKDEKSSLKCVMWRTVAAEFQELFKNGAAVIVHGRIGVYERDGLYQ
                     LYADAVYPVGRGKLFEEFLRLKEKLEKEGLFDSGRKKPLPYMPERIGVVTSSSGAAIQ
                     DIFNTIRKRWPLAEIYLSPASVQGEDAPAELIKAYKKLVEMNPDVILIARGGGSLEDL
                     WAFNDETLVRTIASGKIPVISGVGHETDFTLLDFAADFRASTPTAAAVAATPDRLEII
                     QAVDFLTVKMNRLMREKLDKTAEKLNQLNRRFELAAPLNQIRQEQKILEQIHIRLNLW
                     ADRYFKQQRELLIGLSRRLESAGPSSVLSRGYALIEDENGKIIVSASCFYSDEKVTIR
                     MKDGDRDAMIL"
     gene            complement(11568..12047)
                     /gene="rnhA"
                     /locus_tag="PK898_05295"
     CDS             complement(11568..12047)
                     /gene="rnhA"
                     /locus_tag="PK898_05295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOU19483.1"
                     /translation="MDDKRTKVTIYADGACLGNPGPGGYGVILICGDRKKELSGGAAL
                     TTNNRMELTAVIEGLKALKRPCEVLLYSDSKYVVDMVEGKWPQRWQKNNWMRNKKEPA
                     KNVDLWEELLRLLEIHSVTMKWVKGHAENALNNRCDELAVKAAESFKNSNHNNLSSR"
     gene            12153..12935
                     /locus_tag="PK898_05300"
     CDS             12153..12935
                     /locus_tag="PK898_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU19484.1"
                     /translation="MNETIQTLMNRKSVRKFTDQSVSDDTKQIVLEAAMHAPSAGAMM
                     LYSIIEVEDQALKDILAESCDHQPFIATAPWLLLFVADYQRWFDYYKVSNAPALCAEF
                     GRVPRTPQEGDFLLACMDTLIAAQNAVIAAEALGLGSCYIGDIVEHYEQIRDLFRLPR
                     YAAPIVLLCFGYPTEDQKNRKPVERFDKKYIVHKNQYPSFSDQDLLNMFPKEDIIPNN
                     PLGRLPQGQRNYIRKFTADFSIEMSRSVREMLKNWQQTNPDS"
     gene            12932..15019
                     /locus_tag="PK898_05305"
     CDS             12932..15019
                     /locus_tag="PK898_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19485.1"
                     /translation="MKQKKSEKIKQITLSLIMAVISVQCALSIWRRYQEFLSKGSIVP
                     SFLLICTFWIVGILFIYAILWRPELLKPFLFLKKLPDPIRRMGSFFICLLPIFLYTFH
                     RWSEPFGNPWIRIFLYTVIILAAAWLLTKEKGISGGALIASMLAFSVSFLLINQFRDL
                     TDYPFSVGWSEGNRFWDYSVLFGRRLYNWPQDKPIPAYIDLGRQSLWGAIFLLPKVTI
                     RMMRAWNDFLFTIPYALLGWALLNYRSVTQKKYLLAGSLWAMIFLNQGPIYTPLVLAA
                     ILVALGHKLPLWLGCLFTFLAGAYAVMSRSTWIVAPAIYGGMLTLADTYDKKILSQKR
                     RWFQAILIGLFGIAGAFSYLNQYSIRAFFSESSVTEKSTVTVPLNYGEEYQSVDTSPK
                     ETEASESVPAMLTPAWFQYYLSRQPLLWNRLWPNETYKLGIVPGLLMAVLPLVLLLIF
                     WDHEKHWNLSGMQRLVLGCILFAFLCVGLIVSVKIGGGSNLHNLDMFLIALLIIAAMA
                     WNAGMGEWLIDKVHSGHWINWIILAAIILPVLPAMLTVEPKKYPDPSTTGEALAKVQQ
                     VIAQHAQEEILFMDQRQLLTFGYVPKIPLIADYEKKWMMDEAMADNAEWFKPYIHDLK
                     NQRFAVIISEPLQIKFQGANRNFSEENDLFVKWVSIPTLCYYEPLETFEEQGVQILVP
                     RKSVLVQEGVTCP"
     gene            complement(15036..15338)
                     /locus_tag="PK898_05310"
     CDS             complement(15036..15338)
                     /locus_tag="PK898_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU19486.1"
                     /translation="MKIKPEDARKMLESENPPALIDVREPDEFASGHIPNAVNVPLGD
                     VVNGVIRLGIPKDQPVMVYCRTGRRSSEAASKLQGAGYQKIYDLGGITDWPYEIVK"
     gene            15533..16315
                     /locus_tag="PK898_05315"
     CDS             15533..16315
                     /locus_tag="PK898_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU19487.1"
                     /translation="MITSIQNSKIQWVKKLLTEKKERQESGWYVMEGIRLSEEALQKE
                     IPIQFALYTSPLTNRTENFIKALQKAGIEAEEIPPQLMRSVSDTTTPQGILIVVKAQP
                     LPIQPDPSLVLILDGIHDPGNLGTIFRTAAAAAVDLILLAPGCADPYSPKVVRSGMGA
                     HLKIPFSQSDWREIDDFFQIHPNIIPFASDMKGGKSLWEEDLRRPVAIIIGSEAEGIC
                     KEAERLAKGKIFIPMSPGTESMNAAVAAGILMFEAVRQRSGK"
     gene            16336..16983
                     /locus_tag="PK898_05320"
     CDS             16336..16983
                     /locus_tag="PK898_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19488.1"
                     /translation="MSNIRFIASSESNQPSLSKTDWLDIAVYGELNDDQTALKEEIRP
                     LIQKAVESAVPLRVKASLVLVGNGITSKVHDFSHAGPDRIFLYDHISFSDHNLSDIVI
                     ALKHFSKEYKPSVILFSSSDFSSLLAETLATELNLSFADHISDFKIQPNKDLSSPREE
                     QQPIFQAVAQSRPQIVTVRTETLPIRPVSPRSVGEIILCELPDELFLRQIEKGSS"
     gene            16980..17771
                     /locus_tag="PK898_05325"
     CDS             16980..17771
                     /locus_tag="PK898_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOU19489.1"
                     /translation="MNIIVCIKQVPGTTQVEIDEKTGVMKRDTGSAKMNPYDLYAIET
                     ALRIKEKLIDVQTTAITMGPPQSEAVLREAYMLGIDHGILLTDRKFGGADCLATAYTL
                     SQAILKIGLPDLIICGKQTTDGDTAQVGSELAEFLGLPHIACVLKINSITNQSLEGIM
                     DLGDFTQTVELTFPCLITVDKDICEPRLPSYRKKIATRDRPIQTLTFSDLPDQNPVHY
                     GLDGSATRVMRVFPPVHHKQNQILEGTPDTLASEISKILIKMKVL"
     gene            17779..18972
                     /locus_tag="PK898_05330"
     CDS             17779..18972
                     /locus_tag="PK898_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOU19490.1"
                     /translation="MAGIYIHPENISDFEKVKNLCPFGAIEVSPDGTIQINSGCRVCK
                     VCVKNGEPGAFEYREDVISATDKSEWQGIAVLGEFLPSGEYHPVVFELLGKARELADK
                     VGFRVSLVLIGNEIPAVPDRLWSQGADQVFVYDAPLLKFFDARLYADVVEDFALKQKP
                     SVILVGGTPAGRSLAPRIAARLHTGLTADCTELDIQPNSDLDQIRPAFGGNIMAHIRT
                     PYHRPQLATVRYKIFSPVQPDDSRNGEWTYCDLPEGLFASMKHIASNEKQKVQNIEDS
                     DILIVAGRGFKKKEDLILARQLADELGGELACTRPLIEAGWLEPRRQVGLSGRTVKPR
                     LLIACGVSGSVQFAAGMKSSEMILSINNDPHAAINQIANYSFIGDLYQIIPALLNFIR
                     NGREK"
     gene            18969..20363
                     /locus_tag="PK898_05335"
     CDS             18969..20363
                     /locus_tag="PK898_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOU19491.1"
                     /translation="MNYKKLDEKDIAFLRDVCGEDRVILGKDIPEDFCHDEMEGQSAV
                     PEVMVFVLNATEVSLILYYANQKQIPVTPRGQGTGLVGGAVPIYGGILMNLSKMNRIL
                     ELDEENFTLTVEPGVLIMDIYDFVEAHDLYYPPNPGEKSASIGGNVATNAGGMSAVKY
                     GVTRDYVRGLEIVTPEGDILEIGGKIAKNSSGYSLVQLVTGSEGTLAVITKIILRLLP
                     KPKVQQSLLVPFQSLSEAIQTVPKIIKTKVIPRSIEFMTQDMLQSSEQYLGRRFPDHT
                     GNAYLLLAFDGNSREEVEKDSDQLAELCLREGAIDAFIIDTDERNEAVWKPRSSYLEI
                     IKASTTDMDECDVVVPISRIADYLEFSRKVEEEAGFRVRSMGHAGDGNLHIYACRDHM
                     DETVWKEKLDEFFTKMYTRAGEIGGKVSGEHGIGLAKRRYQRELEGEDVMRLMRRIKL
                     AFDPNEILNPGKVI"
     gene            20455..21006
                     /locus_tag="PK898_05340"
     CDS             20455..21006
                     /locus_tag="PK898_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOU19492.1"
                     /translation="MEQDTFDIELAGIKRTLRLFEVKPGLKIAILNTLGDTELVEACA
                     TEIARRLENVDYDVLVTAESKSIPFAHALSVRTKKPYVVCRKNYKSYMGRALESTSNS
                     ITTQLEQHLILDEKDIPLVKGKKIVIVDDVISTGSTMHAIQTLMEMAGSDPNYTYAAI
                     CTEGNEHDWENIISLGHLPLFEG"
     gene            complement(21150..>23083)
                     /locus_tag="PK898_05345"
     CDS             complement(21150..>23083)
                     /locus_tag="PK898_05345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19493.1"
                     /translation="QWWQLLGSFLGSLLLSAIVFPLKKKIKRKSSMTWLLFLFVWGLA
                     AIIWTSVPTEEVLKNSYFMEITPPNYLPYPASDAAYFGLWSESILAGFGLKDQNVSRQ
                     LFVFGLAVLQRLSGNNILAAINLLTITLALIPAMLFLIGSRLHSHAAGLMAAGIAIFR
                     EFNTLYLAPHFGVSDSKMFLSDLPMMLSLLIFLFAFISWLQKPDSKKQLFLSGILLGL
                     SGLIRSQIFLTIPVIWLILVFHRKDTGKRRFRNFVLFTSSILIVLAPALIRSVSLTGS
                     VALEDMGIHNYELARRLSCDPNWQPTSISGESEAAFAERMKQEMIGFLIDHPGKIVQY
                     TLNHFVKDLVDSWLVLPVGIPPGFTVLNITDSAYQDVPGRLAGGSLVLFAVCAFLIAS
                     GIASLWKRHGIAGLLPLILCPLYMLTAAVGRYSGWRFILPADWVIYFYFCCGVFEFVF
                     WFRSRFIEVTVPESTEARTATGILPRKKLLWNVLLILLILLGWAPPFSGIIPDQLKLN
                     STAENKALIQQIGEHYGGVYADLSTEVNQQDWDVINGRTIYPRWFKAGDGLTSANPWV
                     VNEIRDFNRLSFVLLNEKNRDVILPLRQLPEAVPHRADCFVVGSFTEEGWFEGKAVVF
                     PDTLDSSGRPIIILSNKRD"
     assembly_gap    23084..23183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23184..23569)
                     /locus_tag="PK898_05350"
     CDS             complement(<23184..23569)
                     /locus_tag="PK898_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19494.1"
                     /translation="MDQQIKATPIRKNSIIWGILTMAESSVCMKWLASITADEKNAVF
                     FGFSISRLIMLTLMGILFIGGLIILYGAYRQISFYRRFERAIRNYAFRRILSAFSLIL
                     FFLTAAGLYYLAYIEPIRYYAILERL"
     gene            23635..26193
                     /locus_tag="PK898_05355"
     CDS             23635..26193
                     /locus_tag="PK898_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU19495.1"
                     /translation="MAKKKTNTTKKSTYRGNKKPSMQARKRATQVTNQKKKQNRNTQP
                     IPKTQIIFSALQDIFGTVLILGSTFSLGSLAFQDMNSATAWLAGKMHFVFGQTAVFVG
                     IVGIVLGIWLVFLRRLDKPFSFSWKIFTFCLLVYLFVLVSIQHFAVMQIPFTSSDQAG
                     GIFGEKADNLLIGAFGNGGTLIILFFIATIFVLITWREFLKILPESVGQKLDITEKMW
                     KKAKEIFMVTPAENSREKMKEPEIPIQRFSGESTSPENPSSGNETVTIPGESEYKNDS
                     AKNHFHLSDQKGEFREKERTENKNPEISTKPVQPVVVAATPESMNKPFTPPLLKKIQV
                     KDLSSHKPLTAEKWRLPATDEILDPLKSQQKITPDDPYVQSQVQKIEHVLEIFDAPGK
                     VVDIQCGPTFTQFGIQPGFLDRQDRKVRVRVKQIEALKKDLEMALSVKQLSIDAPIPG
                     KTYIGLQVQNRHRYPVTLREVIESDGFRKYQNGLGIALGKDIYGETYAADLTRMPHLL
                     IAGATGSGKSVCLNAILVCLLMHHSPESLKLVLIDPKRVELTGYNGVPHLITPVVTDV
                     EKVSEILQWVLREMDNRNQHFLEAGVRNIQDFNQKFPEQRMPYIVVVIDELANLMMEA
                     ATEIESSITRLAQTARAMGIHLVVATQRPSRDVITGTIKGNLPSRIAFAVATNTDSLV
                     ILDRVGAEDLFGKGDMYLMSAEDPNLKRLQCVYVSDDEIRRLVDYWRSNPKISEVSAE
                     EIQPKPELTPITLHQPPQKFDGVLTQLPLLDENEPAMKRSADPLLDDAIALVRKQGRA
                     SANMLVSRLSIGFGRANKLLVQMEEMGIIGPPNANPSIPREVLDYGENGTPSKE"
     gene            26195..26641
                     /locus_tag="PK898_05360"
     CDS             26195..26641
                     /locus_tag="PK898_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU19496.1"
                     /translation="MAYFEIGIFQPRNGENIGTLWRSAWQLGAAGIFTIGRKQRKQTS
                     DVLNVPQKIPYRHFDSFEDFLISRPVGTQLIGVEFGGIPLSKFSHPAKAIYLLGSETN
                     GLSKEILAACNAIVEIESINYMSYNVSVAGSIVMYHRLISSIGSSD"
BASE COUNT         7629 a         6171 c         5733 g         7109 t
ORIGIN      
        1 cacggtccgc gaggtacttt aatattccca aacccgaaga aaacaacgca gaaaccgcaa
       61 tgccggccag aaccaggcgt atcacccaat gtccgaaatg aaaacgactt ccgagaaata
      121 tcgaaaaagt caggccagca attccaaaag taatcgccag aatttgaatt tgccattgtt
      181 ttccgctcaa caatacaatc gccaacgcag ctccgaagga agctccctga gaaacgccca
      241 aaaaacccgg ctccaccatc ggattgttta atatcgtctg gaatatcaac ccggctgtag
      301 ctaatgccat ccccataaag agggaaatta ttacccgcgg taatcgaata tggataagaa
      361 tcgtccgatt caaggatgat tcgagcaatg ctctcgggct ttgtaaaccc gggaggggga
      421 atcttcccgc caaacttgaa agaatcaaag caatcaacag cagaatcgcc aatagaaata
      481 attttttggc gggagaatta aaaagattgt tttttatgtc caaagcacaa cctttcttcc
      541 gtccaattgc ataatttcga tcggagtatc ataaatatgt tgcaaattct cttttgtgaa
      601 aacggattcc atcggtccgg ctatgagaac ctgtccgttt ttcattaata ccgccttatc
      661 cgctaaagcc gccgccacat caggctcatg ggtcgacaaa aaaatcgtct ttccggcagc
      721 ggcgagattc cgaacgatat ccaaaaaatg gcgcttattc gccaggtcca attgtgaagt
      781 cgcttcatcc aataagagga tctcagtctc ttgtgcgagt gctctggcta ataagattaa
      841 ttgggactgt ccgccgctga gttttgtgac cggcaaatcc ataaattttc ccatccccac
      901 ttcccggatc gcctcccgcg aaatctcaat atccttttcg gaaggtctct ctaaaggatt
      961 cagccaaggg gttctcccca ttgtgacata ttccaaattg gtatattcaa aggatatctt
     1021 ttcggcttgc gggacaaaac cgattcgttg gccgatttcc cgtctgctga agctttgtaa
     1081 cggctttcct tccaggtata ctccgccgct gtccggctgc aatcttccgt tcgcaatttt
     1141 caggatggtc gtcttcccgg aaccgttagg acctaaaata acgacgattt gtcccgaatg
     1201 cattttgata ttgatcgagg aaagtgaaaa tgaatcggat ttatcatacg aaaattgaat
     1261 atcccgtaac tcaagtaccg tctctttcgg gctcatttct tttttcctcc catcaacaaa
     1321 ataaaaataa ttacgccgac aaaagaagtt atgatcccca aagggatctc cgaggggctt
     1381 aatattcgtg ctccgttatc acaggctaag atcagaatag ctccgattaa catacttccg
     1441 ggaagagaat attgagtgtc agaaccgaat aaccttcggg aaagatgagg gacgatcaac
     1501 ccaacccatc cgataattcc gcttaacgag gttgccgccg aaactgcgaa tacggcggcg
     1561 gtcatcagga gcatctgctc catccttatt cgaactccca aagaggatgc cacttccgaa
     1621 tctaaagcca aaagatttat ccgccaccgg tatttccaca gaatgaaaag acagacacac
     1681 gtaataacaa tcaccaaggg aagcccctgc catatcgtga agcccaaact tcccatcagc
     1741 caaaattcga ttgcatgcag ttgatttcgc gaatcggcga gaaccttcaa cagagataat
     1801 ccagctccga aaagcgcaga aataacgctt ccggataaaa tcaagcgtaa gatccaacca
     1861 ccataatgaa ttttccgggc tgagacgacc gaaaggaaca agccggacat tcccaaaccg
     1921 atcgccaaca attgaacgat ccaaggattg gagtgaaaaa aaacaattcc gattgccgca
     1981 ccgaacgagg caccctgtgt cacgcctaac attccggaat caaccaaagg attattaaac
     2041 accgtttgaa aaaataaacc ggatgatccc aatgccattc ccaataacag acctaaaata
     2101 atacgcggaa tacgcaaatt aaaaaaaatg ttccgtgcgg tagtatcttc ttttaaagtc
     2161 gctggcgaaa gaaacccaag ctcgggataa cggccggcaa atatggagag aaacaaacat
     2221 aaaatcaaaa ttcccgtcaa agcgatcaat tttatccgtt tatttcgttg catcatgcca
     2281 taaccgattc ttaaataatc aatttatgaa tattcaaatt atgccacatt ttcgaaaaga
     2341 cgtataatca aaattcagac ggaagaatag aattctctga tagagcgagt caagcggatt
     2401 tataaaactt atggcagata aaaaagaaat tcttataata accggttcgc gcggcgccgg
     2461 aaagaccgcc tattgccgga atgaagttca aaaagctcga gaaaattcgc tgatcgtccg
     2521 cgggattctc tcacccgcga tgtttttaaa ccataagaaa attgccttct atgcacagaa
     2581 tttaaaaaat gatgaaaaac gtttagccgg atccttggct tcatatgcca atctcggaaa
     2641 acaatttcac gattggacgt tggatcccga tgtaatccga tgggtcaatg aattcttatc
     2701 cgaaaaagaa ccgtgcgatc tgttggtaat cgatgaattg gggccgcttg aattttcgga
     2761 tcacgaagga tatcttgcgg catttgattg tctgaaaaat caaaactatc ggaaagcgtt
     2821 agtcgtcatc cggccggaat gcatcgatgc gtttcagtct tttgggctcc ctttttcaat
     2881 tatcgagatc gagaaagcgg atgccgattc gtgaagatga acgaaaccta ctttccgaac
     2941 aggacattta taaatagccg ctatcaggac aattgcaaat agtattgaca cctattttca
     3001 cccttccttg acggattgat ggaaacggtt ataataattc ccgttgaacg gctggtgtaa
     3061 ggaagaaatt gcgggcatag ttcagttggt agaacacctc cttgccaagg agaaggtcac
     3121 gagttcgagt ctcgttgccc gctcttttac cggaagaaag attacggcga cgtggccaag
     3181 tggataaggc atgggtctgc aaaaccctga ccatcggttc aaatccgatc gtcgcctcag
     3241 agaaatatta cagcacttaa atcctcttaa tcggaggatt ttttattcga tgttcttttt
     3301 cccgtttttt tgaagttcct cgcgagagtt ataaagagcg gtttgagttt atttggattt
     3361 tttgcgaacc ggctttcctt ttaccgggtc cgattttcgg gtatgaccga tccgaattcc
     3421 atatcggggt aagattttat ccgtaaccaa gtaaatgatt accgtgatca atatcgcaac
     3481 cccaaaaaca agtaatgaac tcgaaagatt gttcatcaaa gccggatcaa ttaacatcac
     3541 caacgaaaga gaaaccataa ggcatccgct gaaaagcttt agaatttgcc cgtgtgtttc
     3601 ctgcattcga gacgatttca tcgaaattac cgtaatggtg aaaattacca gttcatccag
     3661 ttgatagaga aacagataaa gaatcaataa tgcgataaaa gttgcttttg atgcgtgatt
     3721 tgcgaccagc aagttgctcc aaaccaccgg aaatgcggcg gtacaggaga attcgaccaa
     3781 cgaaactcct aacgccagaa caatagtcgc tccgatcatt gcccaaagat tttcactgtt
     3841 attaacaaca ttgcgcattt tttgataaat acccggcttt ttttcgtcgg caatcgtcag
     3901 tgaaacacct tctttgaaaa agaaataatc tttcagatta attaaaccca ataccaaagt
     3961 aattacggca acaacaatct ggatccattt caggtaactc gcataagtca acagtgtgaa
     4021 gacaccggtt atgaataaag cgtaaatcaa tgcggccacg ataataaaaa ccaagccgat
     4081 gatcgtcatc tttttccgag aattggtatg gatcagcatg gctaaaagca tcgttaatac
     4141 ccataaggaa cagggattta cgccatccac caatccgata atgactgtgc tgacgaataa
     4201 agtctcctgt gccaaatcaa tctgaccgat caagggaatg gtaattttgg cggataaagc
     4261 cttttcggga ggaattaatt ccgtttttcc gtcaacaatg tcttgggtat caaccacccc
     4321 gttctgaatt ccgctcgaaa tagccgtctc gatttcctgc tgtttgatat cattgaaacc
     4381 gacccaatat tgatttccga taaaagtaac tggaactccg gaaggttcaa acccgtattt
     4441 ttcggcaaat ttttccagaa gtgtcacgtt ttccggatga taccagactt cgtattcgtg
     4501 tatcaccagt tgatcgccat attttgcggt catctgttgc aaaaaaacct cttcctgtgc
     4561 acaatgaggg cagccttctc cccgaaaaaa atataaatta acgatatatt gggttacgga
     4621 actctgtgcc agtccggaga aattcggtat cgcaagaaat ataaaaaggc tgagaataat
     4681 tgagagtaat tttttcttca tagaagtccg gcttcatacc ttcaaatttg ccaccgctac
     4741 aattttaccg taaaagggtt attagggtct ttttctatca atgatctgat tcttactttt
     4801 caaaaacttt tcaaaccatt tgaattccgg agaacgattt tgatgagttt cgcgaaattc
     4861 ttttttcact tctcaataaa attgatgcca taaagtccgc tttgaatgag ttcacctttc
     4921 tccccgatta aaaattccaa aaccggtaaa tcgctgaaat cttccgcttg aaatcggtcg
     4981 ctgagcaacc ataatccttg atttcccgat gcgcagaatc gctccaaagc aggcggattg
     5041 gaatgaatat acggtccgta taaaatcgca tcgatccatg gcaggcaacg atttcgcttg
     5101 ggatcagaca gtattttctc tttttgatat ccgctgaaaa caaaaacgga aagagtcgtt
     5161 tccgctttta tcattcttac cagttcacat aaagcctcag gttgttgaaa tggttcaccg
     5221 ccgctgattg tgataccgtc cacatttttc gcgactgaac ggattttttc gaaaacggtt
     5281 tccaccggat ccgatctgcc gcctttcgga tcttgcaaat cggggttaca gcaaccttcg
     5341 caacctatcg aacatccctg aaaccaaatc acgcagcgct tgccgggtcc gttcgctgaa
     5401 ctttcgggaa taaaataccg ccaccggata ttcatcaaag ttctcttttc gtttttaacc
     5461 gtaatcggga tctattttca accggtttat ccgaataata ttcatcggta tattctcgct
     5521 tgatgacctc attccccaag gcttcctcca gctgtttcgt cgtatccaaa caagccttcc
     5581 ccggaattcc acgaacctga aaggttactt tccccttctc atcaatcaat atctcaattt
     5641 cttcaagctg cgccatttat gctcctaatt taaacgaatg accggataat cttcgaaaag
     5701 acataagccg gatgataaat cataaacggt tatcaataac attttattgc ggtcgacttt
     5761 aaattccgct ttgaatctgg caatcccttt ttccgcggga ggatccgcat gcaggaatag
     5821 cggggcatgt tcattgagcc aaaaagtatc ctctccctct ttctcggtat ctactcggga
     5881 aaaaagtctg attgcgccct cttcgtcgaa aaatatttct gtttgtgctt ccggtcgaaa
     5941 agcgggttgg ctgatttcat agattgccaa accaaaaagg gattgatttt gtcgggacgc
     6001 cttaattttc agagtggtaa ccggttcatc ggaaggataa ggggtaccgg gttgaataat
     6061 cgtatgaaaa cggaactcac cggttttgcc gtcccgaatc cgaaccgcat attgatgccg
     6121 cacaaaatcc aaaactccga aaccatcgat caaatttatc gcacctttgg cgacggcagt
     6181 catcgggtca ccgtttaaga ctaaggcttt acggagaaca tttcccaatt cggaacggat
     6241 cgcaggaatc atactgcctc cgccgaccgg aattaccgcc gtaatcgcag aggcatcgat
     6301 gccgtgatcg gctgcctgta acaacgcatc ccgaactacg gcgtcaactg attgaaacaa
     6361 tccccttttc cttaacaagg tttcaagatc ttcccgagac tgccgcaaag taaattttcg
     6421 atgatccgtt aggattaatg acagatccgc ttcttcgaaa aaggacagtc gctctttcaa
     6481 ttgctcacat gccgccaaaa taattcgtcc gtcccgatga atcaccggat cggaaaaatc
     6541 tcttcgattc tgcatcagcg cttcttcaaa tatccaacgg tctaccgttg atcccccaat
     6601 ggaacaaccg gatttcccga tcacctgaaa agattccggc agaaaacctt cccgcccgtt
     6661 cggttcatgt aagagaacga cggtagcctg catggtgctg cccccgaaat caaagaacaa
     6721 agctggttca cccggatgca tttttaagcc atatccggca gccgccgaaa ccgcttcatc
     6781 cacgatccgg actttccgga agccatcgat cgtaaaacag gaaatcagcc aattcgcata
     6841 atattcgaag gattccaccg gtacggaaaa aaccagttca tcggcatgta cggaaaagcg
     6901 ttcctccgtt tcacggataa gattcgaaag aaactcttcg gcagcttgtt ttgcgctgat
     6961 tttctcgccg ttcactcgga tatgataggg gctgttcatg ccgatatagc gtttcatcca
     7021 gcgaaacgta ttataagaat tttctttttc ctctcggatt acctgacttc ctatgagagt
     7081 ttgcccgtcc ccataatgga tcatggaagg aatcaaaaca cttccgggct cactgatacc
     7141 cggcaaatca atacactgca cctcccgaga agaactttct cggaaagcaa gaactgtatt
     7201 ggtattgccg aaatcaatgg ccaatgtctt cattcgttaa acaatctttt ctgcttcttt
     7261 gaattcagtc gcttttgcct tatcccctgc caacctaatg ataaaataaa tcgatcaaag
     7321 gaaaacgaat tgattatttt ttatcaaagc tgccgaaagg atcattgcca tgcttgaaaa
     7381 aatcataaat gcatttaaaa accgatttgg agccgatccc gatttcgtcg ttcgttcacc
     7441 ggggcgtatc aacctcatcg gcgaacacac ggattataac gaggggttcg ttttcccgat
     7501 ggcaattaaa tatgcacaat ggttggcaat caaagcaaat aaaaccgatc atgtttccct
     7561 ttattctgcg gatatcgata aagatgttgt attctccgtt accgattttt cccaaaaaat
     7621 cacggggtgg gcttgttacc ctcagggcat cgcttgggcg ctgcatgaca acggataccc
     7681 attaaaaggt tgggatggct tgctcttagg agatcttcct ctgggtgccg gcttatcctc
     7741 atcggctgct cttgatttgg ttaccgctcg tgctttctcc ctgaccggcg ggtttcaatg
     7801 ggacccgata cagatgtccg ttatcagcaa gcaatcggaa aatcagtgga tcggcttaaa
     7861 caacgggata atggatcaat tgatctcggc taccggtgaa aaagggaaag ccattctgat
     7921 cgattgtcgt tcattggata ttcaacccca tgctttaccg accgatacga ttattgttgt
     7981 catgaatacg atggtaaaac acgcattggt tgaaagtgga tacaatgatc gcttccggca
     8041 atgtatggaa ggcgccaaag ccttcgggaa aaaattcttg cgtgatgtga caatggagga
     8101 gttcaacgat ggcgccgatc agttagatga ggtgatccgc cgtcgttgcc ggcatgttat
     8161 cagcgagaat gcccgtacgt tggaggctgc cggatgtatg gaaaggaatg atccggtccg
     8221 cctggggcag ttgatgaatg aaagtcatgc cagccttcgg gatgatttcg aagtttcttg
     8281 tgaagaattg gatattatgg cagaagaagg gcagaagcaa cccggttgtt acggcgcccg
     8341 aatgacaggg ggaggattcg gaggcagcgc catcgcactg gtaaaaaccg aatatgccga
     8401 actgtttatg aaaaatatcg cgtcgattta tgaagcgaaa accggtatca aaccgaacat
     8461 atttgccacg gatgccgagc gcggcacatc cgttgaatat accaaagatt gatttctaaa
     8521 ttataaaacg gttcatcaag aacctttttc tttatttgca gatatgaaac atacaagctt
     8581 ttttcactga gtggaagatg tcatcccctg attcagcaaa tgccagactc gatccaaagc
     8641 ttgcaaaccg gttaaattac gaactgtcca gccgtaagtc tgcaaatcat cgccactgaa
     8701 atcacaaagg ctcgtattgt taaatgtggg atgaacatca ttttcacgaa gcccatgccc
     8761 gggtaacgga tccatcgccc gccataaatt ccacaagggc acctcataat ccaaagcaat
     8821 ttgcgcaatt gcttgattga aactgccgtc tccttccaga ttatccgctt ttgtcactaa
     8881 tatcggtacc acatatcgat cgatggattt ttgaaccaac gaacgcatgt gtttatcaaa
     8941 ttcggctaat ggcatgtaga catcattcgt acccaatgcg ataaaagaaa tggatggttg
     9001 ccacaaccgg tattcacaag acgttgcgga ttcatagggc caacagacat catcctcata
     9061 ccaatgatag gtatccagtt gcatcgccgt aataccgttg gctgtcgccc gggattcgcg
     9121 cgaaaaagac cattgaaatt gcttaatcgt ttcttccaga gcggcatact tgcctaattt
     9181 atacccattt tcaccgtaat caaagcatcc caaaaaagaa ggcataatgc tgttactgtc
     9241 accgactttg ctgaaccggt ttgctttgtt gccattttcc tgcccggctc gataaacatc
     9301 gatcatcttt gtgctgaccc tttcgggaag aatcggccag tttttccagt ctgcggcgct
     9361 caacgcagtc ggttgaaccg tcggtatcgc agaagggaca accggactcg gcatcattgt
     9421 ttcggtcggc aaaacggtcg tatgggtggg aatcatttcc gaaatcaggg ttacggtcgg
     9481 caaatcggtc gtttgagaag cacggacacg atgattgttc gacagaaaaa tagacagaac
     9541 caatcctgcc agaatcgcac cgaggataat cgccgcgccg cctaaaatat gaattctttt
     9601 attcattctg tttgtctgtt tcatacccac aaaaaatcta ccgccagcgc caccaaaatt
     9661 ccgagaataa cccctatata aacctgccgc ggagtatgac cgatgacttc tttcaacaac
     9721 tgatcattga tcggttgtcc gctgaaaaca tcccgtaata attgattaat cttctgagca
     9781 tgcagccccg attgccatcg gacattggcc gaatcataag tcacaataac catcaacccg
     9841 aaagccgccg caaaagcagc ggtatcaaat ccttcccata atccgatcga taccgcaatg
     9901 gctgtgacca ttgcggaatg ggagctcggc attccgccgc tctcaatcag taaaacccaa
     9961 tcccattttt tgtttaatgc ataattcagt aacaatttta atagctgcgc aagcatcatg
    10021 gcagccaatc cggaaacaac cgccggatta ccgaaaaatt cagtcagaaa cactgtcttt
    10081 atctccgttt acggtggttt gaccgatcgt ccgaattttc aattcagcct gctccaataa
    10141 gctgctgcaa tatttcgata attgcatacc cgccttatat aactccatcg cttgttctaa
    10201 ttcaaccgtt tcactttcga gttgttccac tattttttga agttgctcga aagcttcttt
    10261 aaatgttaat ttttccgaag aattttccat aattttatcc cgtccttaca aaatcatcgc
    10321 atccctatct ccgtctttca tccgaatagt cactttttca tccgaataga aacatgaggc
    10381 ggaaacaatt atttttccgt tttcatcttc gattaatgca taccctcggc ttaaaacaga
    10441 gctcgggccg gcactttcca aacgacggct caaaccgatt aatagttccc tttgttgttt
    10501 aaaatatcga tcggcccaaa gattcagccg aatatggatc tgttccagga ttttttgttc
    10561 ctgtcgaatc tgattcaacg gagcagccag ctcaaatcgt cggtttaatt gattgagttt
    10621 ttccgctgtc ttatccaact tttcacgcat caatcgattc attttaaccg tcagaaaatc
    10681 aacggcttgg ataatttcca atcgatccgg tgtagccgcg acagccgccg cggtcggagt
    10741 agacgcgcga aaatcagccg cgaaatcgag cagtgtgaaa tccgtttcat gcccgactcc
    10801 gctgataacg ggaatcttgc cgctcgcgat cgttcgcacc agcgtctcgt cattaaatgc
    10861 ccaaagatcc tccaaagaac cgccgcctct ggcaatcaaa ataacatccg gattcatttc
    10921 caccaatttt ttatatgctt tgatcagttc tgcgggagca tcctcgcctt gaacggatgc
    10981 gggactcaag tatatttcag ccaaaggcca ccgtttccga atcgtattga agatatcttg
    11041 gatcgctgct ccgctgctgg acgtgacaac accgattctt tcgggcatat aaggcagcgg
    11101 cttttttctg ccggaatcga acagtccttc cttttcaagc ttttctttca atctcaagaa
    11161 ctcttcaaaa agtttccccc taccgacggg ataaacagca tccgcgtaga gttgatatag
    11221 cccgtctctt tcatagacac cgatccggcc atgaacaatc acagccgcac cgtttttaaa
    11281 caattcctga aattcggcag caacagtccg ccacataaca cattttaagc tggatttttc
    11341 atcctttaaa gtgaaataaa catgaccgga aaccggtctg gataaattgg atatttctcc
    11401 cttaacagaa atatcctcaa gcaccggatc actttccaac aagtctttca cctgttgatt
    11461 cacttccgat acactgagcg caaagtcggc aaaggaaaaa aagtcaaaat tttgcataaa
    11521 accagtatat caccggtagt tttcacttga aattcttttg ataatatcta tcgggatgac
    11581 aaattgttat gattcgaatt tttaaaactt tcagctgctt tcaccgccag ttcatcgcaa
    11641 cggttattca gcgcattttc cgcgtgccct tttacccatt tcattgtgac ggaatggatt
    11701 tccagtaaac gtaagagttc ctcccacaaa tcaacatttt tagccggctc ttttttattt
    11761 cgcatccaat tatttttttg ccagcgctgt ggccattttc cttcaaccat atccaccaca
    11821 tactttgaat ccgaatataa aagcacttcg cacggacgtt ttagtgcctt cagaccttca
    11881 atcacggctg ttaattccat ccgattattt gtggtcagag cggcaccacc cgaaagttct
    11941 ttctttcgat cgccgcaaat caatatcaca ccgtatccac ccggaccggg gttccccaaa
    12001 caagccccgt cggcataaat ggtcactttg gttcgtttgt catccattgc taccggatct
    12061 tctcctctta ttcttctcta aaagcatctt ttatgattcc gcattttttg atcatcaacc
    12121 caataaaatc gaataacgat taaaataagc ctatgaacga aacaattcaa actctcatga
    12181 atcggaaatc cgtccgaaaa tttaccgatc aatctgtttc agatgataca aagcaaatcg
    12241 tcctcgaggc ggccatgcat gcgccttctg ccggcgcaat gatgctttat tctatcatcg
    12301 aagttgagga tcaagcctta aaagatatcc ttgcggagag ttgcgatcac caaccgttta
    12361 tcgcaactgc cccgtggctg cttttattcg ttgcggacta tcaacgctgg tttgattatt
    12421 ataaagtttc aaacgctccg gcgctgtgtg ccgaattcgg cagagttccc cgaactccgc
    12481 aagaaggtga ctttttgctg gcttgtatgg acaccctgat cgctgcgcaa aatgcagtca
    12541 tcgcagcgga ggcactcggc ttgggttcgt gttatatcgg tgatattgtc gaacattatg
    12601 aacaaattcg ggatcttttc cgcttgccac gatatgccgc tccgatcgtc ttattgtgct
    12661 tcggataccc gacggaagac caaaaaaacc gaaaaccggt tgagcggttt gataaaaaat
    12721 atattgttca taaaaatcaa tatccgtctt tttccgatca agacctgttg aacatgtttc
    12781 cgaaagaaga tattatcccg aataacccct tggggcgttt accacagggt caacggaatt
    12841 atattcgcaa atttaccgct gatttttcaa tcgaaatgtc ccgttcggtc cgcgagatgt
    12901 tgaaaaattg gcaacagaca aaccccgatt catgaaacaa aaaaaatccg aaaaaatcaa
    12961 acaaatcact ctgagcctga taatggctgt catttccgtc caatgtgcgt taagcatctg
    13021 gagacgatat caggaattct tgtccaaagg cagcatcgtt ccttcgtttt tgctgatttg
    13081 tactttttgg attgtgggaa ttctttttat ttatgcaatc ttatggcgac cggagctgct
    13141 gaaaccgttc ttgtttttaa aaaagctccc cgatccgatc agacgaatgg ggtcattttt
    13201 catttgtctg cttcctattt ttctctatac atttcatcgt tggagtgaac cgtttggaaa
    13261 tccttggatc cgcatatttc tgtatacggt gatcattctt gccgccgcat ggctccttac
    13321 aaaagaaaag ggaatatccg gcggggcatt gatcgcttca atgttggctt tcagcgtttc
    13381 atttttattg atcaatcagt ttcgtgatct taccgattat ccgttcagcg tcggttggtc
    13441 ggaaggaaat cgcttttggg attattccgt tcttttcgga cgacgcttat ataactggcc
    13501 gcaggataaa ccgattccgg cttatatcga tctcggacgt caaagcttat ggggagcaat
    13561 ctttttactg cccaaagtga caattcgaat gatgcgcgct tggaacgatt ttctttttac
    13621 cattccgtat gcgttgctgg gttgggcatt attgaattac cggtccgtca ctcaaaaaaa
    13681 atatctgctg gcaggctcgc tttgggcaat gatttttttg aatcagggac cgatctatac
    13741 acctttggtt cttgccgcaa tattggtcgc attaggacat aaacttcctc tttggttggg
    13801 atgcttgttc acatttctcg ccggggctta tgcggtaatg tcccgctcca cttggatcgt
    13861 tgctccggca atttacggcg gaatgttaac ccttgccgat acttatgata agaaaatact
    13921 cagtcaaaaa agaagatggt ttcaggcaat tctgatcggc ttgttcggca tagcgggcgc
    13981 tttctcttat ttgaatcaat actccatcag agcgtttttt tccgaatcat ccgtaaccga
    14041 aaaatcaacc gttacggtac cgttgaatta tggggaggaa tatcaatcgg tcgatacatc
    14101 tccgaaagag acggaagctt ccgaaagcgt tccggcgatg cttaccccgg catggtttca
    14161 atattattta tcccgccaac ctttgctttg gaatcggcta tggccgaatg aaacatacaa
    14221 gctcggaatc gtaccgggac tcttgatggc agtcctgcct ttggttcttt tattgatatt
    14281 ttgggatcat gaaaaacatt ggaacctcag cggaatgcaa cgcttggttt taggctgtat
    14341 cctgttcgct ttcttatgtg tggggctgat cgtatcggtc aaaatcggcg gcgggtccaa
    14401 tttacataat ttagacatgt ttctcattgc tctcctcatc atcgcagcga tggcatggaa
    14461 tgccggaatg ggtgaatggt taatcgataa agttcattcc ggacattgga tcaactggat
    14521 tattctggcg gcaatcatcc ttccggttct gccggccatg ttaaccgttg aaccgaaaaa
    14581 atatccggat ccgagtacaa ccggcgaggc actggccaaa gtgcagcagg ttattgccca
    14641 gcatgctcag gaagaaatac ttttcatgga tcagcgccag ttgctgactt tcggatatgt
    14701 tcccaaaatt cctttaatcg ccgattatga gaaaaaatgg atgatggatg aagcaatggc
    14761 ggataatgcc gagtggttca aaccatatat ccacgattta aaaaaccaac gtttcgcggt
    14821 tatcatcagc gaaccgctcc aaattaaatt tcagggggca aaccgaaact tcagcgaaga
    14881 aaatgacctg ttcgttaaat gggtttctat cccgacccta tgctattatg agccgttgga
    14941 gacattcgaa gaacagggtg ttcaaatcct tgtgccgcgt aaaagtgtat tggttcagga
    15001 gggggtaacc tgtccataaa cagaagacac catgcttatt ttacaatttc atacggccaa
    15061 tcggtgattc ctcccagatc ataaatcttc tgatatcccg caccctgtaa ttttgaagcg
    15121 gcttccgaac tccgccttcc cgtgcggcaa taaaccataa ccggttgatc tttcgggatt
    15181 cccaatcgaa tgaccccatt taccacatct cccaacggga cattgacagc attcggtatg
    15241 tgcccactcg caaattcatc cggttcgcga acatcgatca gagccggtgg attctcactt
    15301 tcaagcattt ttcttgcatc ttctggtttg attttcataa caactgttac ctcttcttcc
    15361 ggttttgttt gaccttcaat cgtgccccgt tccgaacaac ccccgagaaa gattgagata
    15421 tagattacaa taaaaaatag aaaaagtctt ttcatggcgc cctttgggga aataggattg
    15481 atgcataatg atggcaaatt ctaacatcag aaatgatttg ggttgaatat atatgattac
    15541 atcgatacag aattcgaaaa tccaatgggt aaaaaaactt ttaacagaaa aaaaagaacg
    15601 ccaagaatcg ggctggtatg tcatggaagg aatccgattg tccgaagaag ctctgcaaaa
    15661 agaaattccc attcaatttg ctctgtatac atcgcccttg acaaatcgta cagaaaattt
    15721 catcaaagca ctgcaaaaag ccggaattga agcggaagaa attcctccgc aactgatgcg
    15781 atccgtatcg gatacgacga ctccccaagg catattaatc gttgtcaaag cgcaaccgtt
    15841 gccgattcag cccgatccga gtcttgtgct cattttggac ggaattcacg atcccggcaa
    15901 tttgggaacg atttttcgga cggctgccgc agcggcggtc gatttaatcc ttctcgcccc
    15961 cggatgcgcc gatccatatt cgccgaaagt ggttcgctcc ggaatgggag ctcatttgaa
    16021 aattcctttt tctcaatccg attggcggga aatcgatgat tttttccaaa ttcatccgaa
    16081 cattattccg tttgcatcgg atatgaaggg aggaaaatct ctttgggaag aagatctccg
    16141 ccgaccggtc gcaatcatca tcggatccga agcggaagga atctgtaaag aagcggaacg
    16201 gttggcaaag ggcaaaattt ttatcccgat gagtccgggt acggaatcga tgaatgcggc
    16261 agtggctgcc ggtattctga tgtttgaagc agtccggcaa cgatcgggga aataaaagca
    16321 cggaggtgaa atcgattgag taacattcgt tttattgcgt catccgaaag caaccaacct
    16381 tctctgagta agacagattg gctggatatc gctgtctacg gcgagttaaa tgatgaccaa
    16441 acggcattga aagaggaaat ccgcccgttg attcaaaaag cggttgagtc ggcagttcct
    16501 ctgagagtaa aagcttcatt ggtactggta ggaaacggaa taacttccaa agtccatgat
    16561 ttttctcatg cgggaccgga tcggatcttt ctatatgatc atatttcctt ttcggatcac
    16621 aatctttcag atattgtgat cgcattgaaa catttctcga aggaatataa accttcggtc
    16681 attctttttt ccagttccga ttttagctct cttcttgcgg aaaccttagc taccgaattg
    16741 aatctgtcct ttgccgatca tatttcggat tttaaaatac agcctaataa agacctatct
    16801 tcccccagag aagaacaaca gccaatcttt caggcggtcg ctcaatcgag accacagata
    16861 gtaacggtcc gaacagaaac acttccgatc cggcccgtca gcccacgctc cgtcggtgaa
    16921 ataattctat gcgaattacc tgacgagctt tttctccgac aaatcgaaaa aggttcatca
    16981 tgaacataat cgtttgcatc aaacaggtcc cgggtacgac acaagttgaa atcgacgaaa
    17041 aaaccggcgt gatgaaacgg gacaccggtt cggctaaaat gaatccttat gatctatatg
    17101 cgattgaaac cgcactccgg atcaaggaga aacttatcga tgttcaaacc acagcgataa
    17161 cgatggggcc tccgcaaagt gaagccgttt tgcgcgaagc ctatatgctc ggaatcgatc
    17221 acggaattct gttaaccgat cggaaattcg gcggtgccga ttgtttggca acggcatata
    17281 cactctctca ggcgattttg aaaatcggtc ttcccgattt aattatctgc ggcaaacaaa
    17341 ccactgacgg tgacacagct caagtcggtt cggaattagc tgaatttttg ggacttcccc
    17401 atatcgcctg tgtcttgaaa atcaattcga tcacaaatca atccttggaa gggattatgg
    17461 atctcggtga cttcacacag actgtcgaac tgacatttcc gtgtctgata acagtcgata
    17521 aggatatctg cgaaccgcgt ttaccgtctt accgaaaaaa aatagcaacc cgagatcgtc
    17581 cgattcaaac tcttactttt tcggatttac ccgatcaaaa tccggttcat tacggtttgg
    17641 acggttccgc gactcgggtg atgagagttt ttcctccggt acatcataaa caaaatcaaa
    17701 tcctggaggg aacgccggat acgcttgcgt cggaaatcag caaaatcttg ataaaaatga
    17761 aagttctctg aggcgaccat ggccggaata tatatccatc cggaaaatat ttccgatttt
    17821 gaaaaagtga aaaacctatg cccgttcggt gcgattgaag tgagtccgga cggaacgatt
    17881 caaatcaatt cggggtgtcg tgtgtgcaag gtttgcgtaa aaaacggcga accgggagcg
    17941 tttgaataca gagaagatgt gatttccgcc accgataaat ccgaatggca gggtatcgct
    18001 gttctcggtg aattccttcc ttcgggagag taccaccccg ttgtattcga attgttgggg
    18061 aaagcgcgag aattggcgga taaagtcggc ttcagggtct ctcttgtttt gataggcaat
    18121 gaaattccag ccgttcccga tcgtttatgg tcgcagggag cggatcaggt attcgtctat
    18181 gacgctccgt tgctgaaatt ttttgatgcc cgtctttatg cggatgttgt tgaagatttc
    18241 gcccttaaac agaaaccttc cgtcatattg gtcggcggaa caccggccgg tcgctccttg
    18301 gcaccccgta tcgccgcccg tttacacacc ggtttaacag cggactgcac tgaattggat
    18361 atccaaccca attccgatct ggatcaaatc cgcccggctt tcggcggaaa tattatggca
    18421 catattcgca cgccttatca tcgaccgcag ctcgccaccg ttcgctataa gattttttca
    18481 cccgtccaac ccgacgattc acgcaacggc gaatggacat attgcgacct accggaagga
    18541 ttattcgctt cgatgaagca catcgcttca aatgagaaac aaaaagttca aaatattgaa
    18601 gattccgata ttttgatcgt tgccggacgg ggtttcaaga aaaaagaaga cctgattctc
    18661 gcccggcaat tggcggatga attgggagga gaattggctt gtacccgacc tctgatcgaa
    18721 gccggatggt tggaaccacg ccggcaggtg ggcctttccg gaagaacggt aaaaccgcgt
    18781 ttattgatcg cctgcggggt ttccggttcc gtccaatttg ccgcggggat gaaatcgagt
    18841 gaaatgatcc tttcaatcaa caatgatcct catgccgcta tcaatcagat tgcaaattat
    18901 tcatttatcg gagatcttta tcaaatcatt ccggcgttat tgaatttcat caggaacggg
    18961 agagaaaaat gaattataaa aaattggatg aaaaggatat cgctttcctt cgggatgttt
    19021 gcggtgaaga tcgggtaatt ctcggcaaag acataccgga ggatttctgt catgacgaaa
    19081 tggaagggca atccgccgtt cccgaagtta tggtttttgt tttgaatgcg acggaagttt
    19141 cattgatcct gtattatgcc aaccaaaaac agattccggt tactcctcgc ggtcaaggga
    19201 ccggtttggt cggcggagct gtaccgatct atggcggaat tttaatgaac ctctccaaaa
    19261 tgaaccgaat cctggaattg gacgaagaaa atttcacatt aaccgtcgag ccgggcgtat
    19321 taatcatgga tatttatgac ttcgtcgaag cgcatgacct ttactatcct cccaatcccg
    19381 gcgaaaaaag cgccagcatc ggcggaaatg tcgcaaccaa tgccggagga atgtcagcag
    19441 tcaaatatgg cgtaaccaga gattatgtcc gcggattgga aattgttaca ccggaaggag
    19501 atattctcga aattggagga aaaatcgcaa aaaacagttc gggatattcc ttggttcaat
    19561 tagttaccgg ttcggaaggg acgctggcag ttattacaaa gatcatctta agattattac
    19621 cgaaaccaaa agtccaacag agtttgctgg tcccctttca atccctgtcc gaagcgattc
    19681 agacagttcc gaaaattatt aagacgaaag tcattccccg ttcgattgaa tttatgacac
    19741 aagatatgct gcaaagcagc gagcaatatc ttgggcgccg ctttcccgat cataccggta
    19801 acgcttattt gttgctcgcc tttgacggaa acagccggga agaagtggaa aaagacagcg
    19861 atcaattggc tgaattatgt ctccgagagg gagcgattga cgctttcatc atcgacacag
    19921 acgaaagaaa tgaggcggtc tggaaaccgc gcagttccta tttggaaatc atcaaagcct
    19981 cgaccacgga tatggacgaa tgtgatgttg tcgttccgat cagccggatc gcggattatc
    20041 tggaattttc tcgtaaagtc gaagaagaag cggggtttcg agttcgctct atgggacacg
    20101 cgggagacgg taatttacat atttacgctt gccgggatca tatggatgaa accgtctgga
    20161 aagaaaaact ggatgaattt tttactaaaa tgtacacgcg tgccggcgaa atcggcggaa
    20221 aagtctccgg cgaacatggt atcggccttg ccaaacgccg ttatcagcga gagctggaag
    20281 gtgaagatgt catgcgtctg atgcgccgta ttaaactggc ttttgatccg aatgaaattc
    20341 tgaatccggg aaaggtgatt tgaacggata caagcggggt tatgttataa tcttagcgtt
    20401 aaccgaaaaa ataccgatgg ctaacacaag ttaaaatcag gagtgtaaga aaagatggag
    20461 caagatactt ttgatattga gttagcaggg attaaaagaa cccttcgtct atttgaagtg
    20521 aagcccggct taaaaatcgc gattctgaat acccttgggg atacggaatt ggtggaagct
    20581 tgtgcgaccg aaatcgcaag gcggttggaa aatgttgatt atgacgtgct tgttactgcg
    20641 gaatcgaaga gcatcccatt cgcacatgca ttatcggttc gcacgaaaaa accttatgta
    20701 gtctgccgaa aaaattacaa atcctatatg ggacgggcac ttgaatccac ttcgaattcc
    20761 attacaaccc aactggaaca acatttgatt ctggatgaaa aagatattcc tttggtaaag
    20821 gggaagaaaa ttgttattgt ggatgatgtc atcagcaccg gatcaaccat gcatgccatt
    20881 caaacactga tggaaatggc cggaagcgat ccgaattaca cgtatgctgc gatttgcacc
    20941 gaagggaatg agcacgactg ggaaaatata atctccctgg gtcatcttcc tctgtttgaa
    21001 ggctaatcgt aacggaaata tttcctgatt gattcgatct gttttcgaag atgatttccg
    21061 aacgaaaaga agggaattaa tatagaagtg agttattcat agcggggcgc caatcgacgt
    21121 cccggcttct ttcaacacag caaatttgtt taatctcttt tattgctcag aatgattatc
    21181 ggtctgccgc ttgaatccaa agtatcgggg aaaacgaccg cttttccctc gaaccaacct
    21241 tcttccgtga aactgccgac gacgaaacaa tccgcccgat gaggaaccgc ttcgggaagc
    21301 tgccgcagag gtaaaatcac atcccgattt ttttcattca gaaggacaaa gctcagccga
    21361 ttaaaatcac gaatttcatt aaccacccac gggttggcac tggttaatcc gtctccggct
    21421 ttaaaccaac gaggataaat cgtccggccg ttaatcacat cccaatcttg ttgattcacc
    21481 tcggtagaaa gatctgcgta aacgccgccg taatgctcac cgatttgttg aatgagcgcc
    21541 ttattctccg cggttgaatt gagtttaagc tgatccggaa tgattccgct aaacggtggc
    21601 gcccatccga ggagaattaa caggatcaat aagacgttcc ataaaagctt tttccgaggc
    21661 aagattcccg ttgcggttct tgcttcggtc gattcgggaa cggtaacctc tatgaatctc
    21721 gatcggaacc aaaagacaaa ttcgaatacc ccgcaacaga aataaaaata aatcacccaa
    21781 tcagccggta atataaaccg ccagccggaa taacgcccca ctgcagccgt cagcatgtat
    21841 agaggacaaa gaatcaaagg gagcagaccg gcgatgccgt gacgtttcca aagagatgca
    21901 attccgctcg caatcaaaaa tgcacagacg gcaaatagaa cgagcgatcc tcctgcaagt
    21961 ctccccggaa catcctgata tgccgaatcg gtgatattca aaacggtgaa ccccggcgga
    22021 attcctaccg gcagcaccag ccaactgtca actaaatctt tcacaaagtg attcagtgta
    22081 tattgaacaa ttttccctgg atgatcgatt agaaagccga tcatttcctg cttcatacgt
    22141 tcggcaaatg ccgcttcact ttcgccggat attgaggtcg gttgccaatt cggatcacag
    22201 gataatcgtc ttgccagctc ataattatgg attcccatat cttccaaagc tacacttccg
    22261 gttaatgaga cgctgcgaat caaggcagga gccaaaacga tcagaatcga agatgtaaac
    22321 agcacgaaat tccgaaaccg ccgcttccct gtgtcctttc gatgaaaaac taatattaac
    22381 cagataaccg gtatcgtaag gaatatttgt gagcgaatta acccgcttaa tcccagtaaa
    22441 attcccgaaa gaaacagttg ttttttcgaa tcgggttttt gcagccaaga aatgaaagcg
    22501 aacagaaata ttaagagtga cagcatcatc ggcaaatcag ataggaacat tttcgaatcc
    22561 gacacaccga aatgcggagc caaatataac gtgttaaatt ctctgaaaat cgcaattccg
    22621 gccgccatta agccggctgc atgagaatgc aaccggctgc caatcagaaa caacatcgcc
    22681 ggaatcaaag ccaaagtaat tgtcagaaga ttgatcgccg ccaaaatatt atttccgctg
    22741 agtcgctgca gaaccgccag tccgaataca aaaagctggc gggaaacatt ctgatccttt
    22801 aatccgaaac ccgccaaaat actttccgac caaagtccga aatatgccgc atccgaagcg
    22861 ggatatggaa gataattggg cggtgtaatt tccataaaat agctgttttt aagcacttct
    22921 tccgtaggaa cagaagtcca tattatggct gccaatcccc agacaaacaa aaataatagc
    22981 catgtcatgg aggactttct ttttatcttt tttttcaacg ggaaaacgat tgcactcagc
    23041 aacaatgaac cgagaaaaga accgagaagt tgccaccact gtannnnnnn nnnnnnnnnn
    23101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnttcaatc gttcgagaat cgcataataa cgaatcggtt
    23221 caatatatgc gaggtaatat aaaccggcgg ctgtcaagaa aaaaaggatc aatgaaaaag
    23281 cggataaaat ccttcgaaaa gcgtaatttc gaatcgctct ttcaaaccgt cgataaaatg
    23341 atatttgtct gtatgcgcca tataaaataa tcaacccgcc gataaataag attcccatca
    23401 gcgttaacat gatcagccgt gaaatcgaaa atccgaaaaa aaccgcattt ttttcatcgg
    23461 cggtgatgga tgccagccat ttcatgcaaa ccgatgattc tgccatcgtc aggatacccc
    23521 agatgattga attttttcgg attggcgtgg ctttaatttg ttgatccatg tttgattccc
    23581 ttcaagctgt atcgataatt ttacctcgtg gagtgctaat gttatacttt cagtatggca
    23641 aaaaagaaga caaatacgac gaaaaaaagt acttatcgcg gaaataaaaa gccctccatg
    23701 caggccagaa aacgggctac tcaagtcaca aaccaaaaga aaaaacaaaa tcgaaataca
    23761 cagccgattc cgaaaaccca aattattttc agcgccctgc aggatatttt cggtactgtt
    23821 ttaattctcg gaagtacttt tagtctgggg agcctggcat ttcaagatat gaattccgcc
    23881 acagcttggt tagcgggaaa aatgcacttt gtatttggac aaactgccgt ttttgtcggc
    23941 atcgtcggaa ttgttttggg aatatggttg gtattcctgc gtaggttgga caagccgttt
    24001 tctttttctt ggaagatttt caccttttgc ttactggttt atttgttcgt ccttgtttcg
    24061 attcaacatt ttgccgttat gcagattcct ttcacttctt cggatcaagc ggggggtatt
    24121 tttggcgaga aagcggacaa cctgctgatc ggtgccttcg ggaacggagg aacattaatt
    24181 attctgttct ttatcgccac catttttgtg ttgattactt ggcgcgaatt tctcaaaatt
    24241 cttcccgaat ccgtcggtca aaaactggat atcaccgaaa aaatgtggaa aaaagctaaa
    24301 gagattttta tggttactcc cgctgaaaac agccgagaaa aaatgaagga acccgagatc
    24361 cctatccaac gattttccgg cgaatcaact tcaccggaaa atccttcttc cggaaatgaa
    24421 accgtgacaa taccggggga atcggaatat aaaaatgatt ctgccaagaa tcactttcat
    24481 ctatccgatc aaaaaggcga attccgcgaa aaagaaagaa cggaaaataa aaatccggaa
    24541 atttcgacaa aaccggttca gccggtggtt gttgccgcta cccccgaatc gatgaataaa
    24601 ccgtttactc cgcctttgct gaagaaaatc caagtaaaag acctttcttc tcataagccg
    24661 ctgacggcgg aaaaatggcg gttgcctgct acggatgaaa ttttggatcc gcttaaatct
    24721 cagcaaaaaa ttactcccga cgatccttat gtacaaagcc aggttcaaaa gatagaacat
    24781 gttttggaaa tatttgacgc gccggggaag gtagttgata tccaatgcgg tccgacattt
    24841 acgcaattcg ggattcagcc cggttttctg gatcgtcaag accgaaaagt ccgggttcgg
    24901 gtcaaacaaa ttgaagccct aaaaaaagac ttggagatgg cactgtctgt taaacagctc
    24961 tccattgacg ctccgattcc cggaaagacc tatatcggac tgcaggtgca gaatcggcat
    25021 cggtaccccg ttaccttacg agaagtaatt gaaagcgacg ggtttaggaa atatcaaaat
    25081 gggttgggaa tcgcattagg gaaagatatc tacggagaga cttatgcagc cgatctgacc
    25141 agaatgcccc atttgttgat tgccggtgcg accggctccg gtaaatcagt ttgtcttaat
    25201 gcaattttag tctgcttatt gatgcatcat tctccagaga gcttgaaatt ggtgttgatc
    25261 gaccccaaac gggtggaatt aacgggctat aacggtgtcc cgcatctgat tacaccggtt
    25321 gtgaccgatg ttgaaaaagt cagtgaaatt ctacaatggg tgctgcgtga aatggataat
    25381 cgcaaccaac attttctgga agcgggcgtc cgaaatattc aggatttcaa ccaaaagttt
    25441 cctgagcaaa gaatgcctta tattgtcgtg gtaatcgatg aattggcgaa cctgatgatg
    25501 gaagccgcca cggaaattga aagcagtatt acccggcttg ctcaaactgc ccgcgcaatg
    25561 ggaattcatc tggtggtggc tactcaaaga ccgtcgcggg atgtaattac gggaaccatc
    25621 aaaggcaatc tgccttcgag aatcgcattt gccgtggcaa caaatacgga tagcctggtc
    25681 attttggatc gcgtgggagc ggaagatctg tttggaaagg gagatatgta tctgatgtcc
    25741 gccgaagatc ccaatttaaa acgcttgcaa tgtgtatatg tttcggacga tgaaatccgg
    25801 cgcttggtag attattggcg ttcgaatccg aagatcagtg aagtctccgc cgaagaaata
    25861 cagccgaaac ctgaattaac accgataacc ctccatcaac ctccgcagaa attcgacggt
    25921 gttctgacac aattaccgct tttggatgaa aacgaaccgg cgatgaaaag gagtgctgat
    25981 ccattgcttg atgatgcgat tgctttagtg agaaagcagg gtagagcctc tgccaatatg
    26041 ttagtcagtc ggctgagtat cggtttcgga agggcaaata aactattggt gcagatggaa
    26101 gaaatgggca tcatcggtcc tccgaatgca aatccgtcca ttccacgtga agttttagat
    26161 tacggagaaa acggaactcc gtcgaaagaa taatatggcc tattttgaaa tcggaatttt
    26221 tcaaccgcgg aacggggaaa atatcggcac tttatggcga agcgcatggc aactgggagc
    26281 cgccggaata tttaccattg gcagaaaaca gcgaaaacaa accagtgacg tgctgaatgt
    26341 cccgcaaaaa atcccttatc gccatttcga ttcttttgaa gattttctga tttcacgtcc
    26401 cgtcggcaca cagttgatag gcgttgaatt tggcggaatt ccgttatcaa aattcagcca
    26461 tcccgcgaaa gcaatttatc tgctcggttc cgaaacaaac ggattatcga aagaaatcct
    26521 tgctgcctgt aatgcgatcg tcgaaatcga atcgataaat tatatgtcat acaatgtttc
    26581 cgttgccgga tcgattgtga tgtatcatcg attgatttct tcgatcggat catccgattg
    26641 attctttgtt tccggtatcg ttgtcttcgg taagaacaat aaaccgatac aaaagatacc
    26701 gatcagcatg accccgtatt gatagcgcat atcctgagca at
//
LOCUS       DAOSMM010000025        25602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_1923_length_25602_cov_17.560457, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000025 DAOSMM010000000
VERSION     DAOSMM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 25602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25602
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1923_length_25602_cov_17.560457"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            663..1604
                     /locus_tag="PK898_05365"
     CDS             663..1604
                     /locus_tag="PK898_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOU19497.1"
                     /translation="MKKRIYILIQILVILFAKVGFVGAQDQKLNILTTIFPIYDFTRT
                     VAGDFAEVSMLLPPGADSHSYEPTPRDILKIQNADLFIYIGGESDIWVDQILSSLGDK
                     KPRTLVLIQSVNALPEEVVPGMQAEEESQSSDESDVVYDEHIWLSPLNAIKMADAIAA
                     SLTESDPAHAPDFESHRAALDQKLQELDQTFRDIVKSAKRKTIIVGDRFPFRYLTEEY
                     GLEYYAAFPGCSAEVEPSASTVAFLIDKVKTEKIPVVFYIEFSTHKIADAIADATGAK
                     TMLLHSAHNVSQEDIDSGITYLSIMTENAEKLREALN"
     gene            1662..2507
                     /locus_tag="PK898_05370"
     CDS             1662..2507
                     /locus_tag="PK898_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOU19498.1"
                     /translation="MSQLIHSIQNLFLDDLGKTNLLGKIILAILIYAVMKIVLLIINK
                     ALRRFSSHKFPKVPESKKITVFSALNSFVKFIIYFIAITFILDLFGINTSSIIATAGI
                     GGIAIALGAQTLVKDILSGLFILLEDQFNIGDYLTIGSVTGTVEEIGLRKIKVRDDDG
                     SITLIPNSLVQQVTNYSTHPIKINVKFPVPYAIKPDEIGAMIEKISEECRKEVDYFTA
                     APAFSGIDSLADSNYTVQVAAETISGNQGAGQQLIKQKILKEIQDHGIFSAAVDENSE
                     KAQQP"
     gene            2587..2913
                     /locus_tag="PK898_05375"
     CDS             2587..2913
                     /locus_tag="PK898_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOU19499.1"
                     /translation="MKVIFHVDEPEKWQLALENVRNFLNAEPEAEIEVLANSAGVKFY
                     IQEASTNDLFVELVGEGVLFAACNNALRKFGFRKEELPEAVIIVPAGVVELAERQEKG
                     FAYIKP"
     gene            3102..3902
                     /locus_tag="PK898_05380"
     CDS             3102..3902
                     /locus_tag="PK898_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU19500.1"
                     /translation="MKINYQETTNDLLTRIDIHNKYGSKDIDQWMLDTLDLKPGIKIL
                     DVACGGGKQCISFYKYLKGDCDITGGDVNQELLDQAELENVKLGSPFKVIPLDFDKRF
                     PFDDQTFDLVSCCFAIYYASDIPFTIREMHRVLKPGGRIFTTGPMPDNKKVFYDIIRE
                     ATGKEIPPMPGSSRYSTEIYDAIAQTFSKVESHIFENPLIFKQPGPFIAYTRASLSED
                     RRLWTSLFENSDEFEKMMQKILKVADERIKRDGELVMTKVVGGFIGTK"
     gene            3904..4548
                     /locus_tag="PK898_05385"
     CDS             3904..4548
                     /locus_tag="PK898_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOU19501.1"
                     /translation="MSEMRFYGKRILFIGAHPDDIELGCGAFIAQIASQTEVRCVTLS
                     DNQKDPALKNLPDEHFAAMAALGVKPDRVSLLAYETRRFPFFRQEILEEMVKLQREFQ
                     PDIVFVHTSADVHQDHKTVTEEALRAFRGRTVLGFDVIRSSYGFFPSFLVSVTEEQVN
                     RKIESLRCYQTYQSKYYFDPEITRATLIRNGAICERKYAEGFDILRIVASFGKD"
     gene            4554..6212
                     /locus_tag="PK898_05390"
     CDS             4554..6212
                     /locus_tag="PK898_05390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19502.1"
                     /translation="MAWKNIKKTTLTNFWKIVTVVFFILLLGIGIVIHDDYGGYTDEL
                     IEIQTGMIQLKYVFSKIPMLEDISGLNIPLYGNFAALPDLATYEDRIYGSAAMLPTFF
                     VNYLPGISLDVGEFLNFRRFYNFLNFFLAAILFYLFLQKRFRNPLIALTGTLMLVLSP
                     RIFAESFYNCKDILFLSWFLISLLGIGFYQLDRKKYGLVLFGFAFGLAANTRFYGFIL
                     LPAFAGIAIFDMITDPKNRKKILRQTFLTCIASIVWFFILTPFLWESDFQQIIGGLKV
                     AVDQPVIGDAELFMGRFVAPRDVWYYLPVWIGITVPVLYLALFVIGSAFLPFKIQSLK
                     DSDTRFELAFDIMNFLLFVIAIMAIVISKGTIYHGWRHAYFLYAPFIYISVCGLNDLI
                     EFKISGKTKMKIKNGIVFSLCLLSFANTGWWMIKNHPLDFVYFNEIGKNYAEDFTRDY
                     WGVASKSCLLYLYNAVQVGEIRVGVNSDLTYGSAEITLLRLPEAVRKRFTPVWQMKNA
                     DYLCYSYKNTPGNDYQIPDFAVMKTFMVDGYAVMGVYQRINPAE"
     gene            complement(6254..7069)
                     /gene="jag"
                     /locus_tag="PK898_05395"
     CDS             complement(6254..7069)
                     /gene="jag"
                     /locus_tag="PK898_05395"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOU19503.1"
                     /translation="MEFSAGTVDEAIEKGLKALGKTRDEVDIVVIDSGAKGIFGIGSR
                     PASVSLKVKESAAKAPQSESSQTGRTVKPESDKISAKEISEELPGDVNEADTTEAESE
                     NEEEKTAGEELVDETALKATVTVVEDLIDKMHIKASVTGRIGKLETSGNQEMILVDIK
                     GDDLSYLIGRHSETLNAFQYITSLIVGRELGHWVPMMIDVQGYRERRERQLRQMAIHM
                     ADQVTRSGRRISLEPMPGTERRIIHLALRDRNDIYTESVGEEPNRKVVIYPKN"
     gene            complement(7110..8051)
                     /locus_tag="PK898_05400"
     CDS             complement(7110..8051)
                     /locus_tag="PK898_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOU19504.1"
                     /translation="MWQSFVNIFLNILLYIYKLVGNFGVAIILFTVLIKLITYPLMRK
                     QIQSSTKMMELQKSPEYLEMMEKYKGNQEKLAEEQMKLYKKFGVNPTASCLPTLIQLP
                     IIFALYQAIIAAIVSTPLGMLDLTKRAFSGFLQIGEIFPINPNFLWMNLGQPERLYIQ
                     GISFGIPVLAILVVITTIMQTKLTVPPTNPNDKNAASGAMMTGMMNIYMPLLMGFMAY
                     TLASGLSLYFLISNIFTIVQYGVEGKLNWKALKFGKPQMPKLATPAEKPVTTSRPVAS
                     ANPKKSMTPNLAESGIEPAEEKIDYREFKNKKKPKKK"
     gene            complement(8064..9218)
                     /locus_tag="PK898_05405"
     CDS             complement(8064..9218)
                     /locus_tag="PK898_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU19505.1"
                     /translation="MKRKKQFLLVLLMILTTMILSGCAGNSAMSNAVSWPGLSADDET
                     VYVAYTSSVQAVSNGKLLWKYPAEAQNGMMFFAAPTIYDGKIYTGTYQNEVHILDQND
                     GTLITKITLETNKNKILASPVIADDMLFIASNNGSVYAYTLDNLSVPVWQTNLSTEIW
                     VSPFVSDGKVYVTTLDKRFLVLDEKTGTIEKALSISGAVMGKLVFANDRIYFGTLGKE
                     VAYFDPATEEIKTVMHTNGEIWASPLIIDGKIIVADMSGYLYCNESESGKSIWQIKAV
                     STDKVGFIADPILLQNGNLLAVAEDGNLMVYDTDGKSVNSRLINGKTMSSPVVIGESF
                     VAGLIPGDALLRSYTSDLKEDWWYIETVESTALTPTAEVSQETVTETPAK"
     gene            complement(9243..9593)
                     /gene="yidD"
                     /locus_tag="PK898_05410"
     CDS             complement(9243..9593)
                     /gene="yidD"
                     /locus_tag="PK898_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOU19506.1"
                     /translation="MIHIAQKDKMIRAISSLFRRKESDCSQDAYAGEPRLSELPFRIS
                     NLPRFFFLILIRIYQNTISKLLPANTCRFYPSCSHYAYQAIYKYGAFKGVPMAIWRVL
                     RCNPFNPGGFDPVP"
     gene            complement(9595..9945)
                     /gene="rnpA"
                     /locus_tag="PK898_05415"
     CDS             complement(9595..9945)
                     /gene="rnpA"
                     /locus_tag="PK898_05415"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOU19507.1"
                     /translation="MKRKKRLNRSDDIQRVRQRGKSFSHPMVVLTTLMDPNIGESKIG
                     IIATKSVGGAVSRNRSKRLIRAAIEPYTDQIKPNYHLVLIARKPILEADIENVSKAVY
                     SLLKKAEVLLPSSQ"
     gene            complement(10020..10193)
                     /gene="rpmH"
                     /locus_tag="PK898_05420"
     CDS             complement(10020..10193)
                     /gene="rpmH"
                     /locus_tag="PK898_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOU19508.1"
                     /translation="MSTKRTYQPKKRRRLRVHGFRERMETASGRDVLKRRRARGRHNL
                     TVSMNNHVKRIRW"
     gene            complement(10554..12074)
                     /locus_tag="PK898_05425"
     CDS             complement(10554..12074)
                     /locus_tag="PK898_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19509.1"
                     /translation="MKKQTLSILLALVLLLSISIFPVFAQELSEDEVSGYFGAIEDWT
                     TQDLALAGVDSEAVDLTEFEAKSVENPVPTAELTDEVTVEGEKGVDLQAKSIIDPATV
                     TLSGEFVACGDGKIKIEIGLKRTADSSSTGMPNMIFVKSVIVTIGTTDYELQNCKSQY
                     GDACSKVYLDASGNATLTGYVEIPRVLDTTPVSFDVEINYSKTAHGALETPVTKSGGS
                     ATSTKDLCATEGVDVVATKGIYEQCGGKASFKADIEVPDEDGPECLVPAQIYVEGTSY
                     SNYTCRYTRFGSEGYPFGGDYCTPGKAIPVKPGDKIRFEAIINDLTNPVAETSGGADL
                     NVNWRFGYNPDMISGVMESVPGPAELCDAKIVPLKPLDSMEDANDPIEPSGWYNTYDK
                     AVVGLYQKCGKFAVMQIRLRNDGAKTGFINLPGSVAVNGGAPIAFYWLSSVAPHGNKV
                     ALGPGEIVTLVGRIWLTNLISQTNSDGAANVAVNFSDLGLYMTGSLFSDRVNWRCY"
     gene            complement(12381..13772)
                     /locus_tag="PK898_05430"
     CDS             complement(12381..13772)
                     /locus_tag="PK898_05430"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOU19510.1"
                     /translation="MRDIIDTKTDIEIKTSPKIPAGAIRLQIVSGAKSELLKEKGDSV
                     VEKEKAKTTVSFYTGEKINADTYRSLGGIIAKWIKKNGIKTAALQIPADLNDKDHKAL
                     LEGLLLGSFSFDKYKKKEDPVDSVIYLDPSEEILLQTCKTVCDAVNMAREWAHEPAAI
                     INPQTLSERAQKFAKQFGLNCKILDDKQLTEMGAGAIVAVGQGSKTPSRMIILEYKGT
                     EKEKPVVLVGKALTFDSGGYSIKPGDSMVGMKYDKCGAMAVLGVLRAASQLKIATPII
                     GIICAAENMLSQNSYRPDDILISLSGKSIEVISTDAEGRLVLADGLTYAQQNFSPQAI
                     IDLATLTGGCAVAYGHVRAGVLTNNQSLYRELFESGERTWERIWQLPNDEDYAKLIKG
                     KDADIKNSGGRLASPVTGGMFLKEFVSDEVPWAHLDIAGVADLDEGTDYSVPGATGFG
                     VRLLIDWLSNRNQ"
     gene            complement(13929..15380)
                     /gene="gcvPB"
                     /locus_tag="PK898_05435"
     CDS             complement(13929..15380)
                     /gene="gcvPB"
                     /locus_tag="PK898_05435"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOU19511.1"
                     /translation="MDATEKTIFELSANGRKGFRFPSSDVPASDLPESLLRKELPLPE
                     LSEVDVVRHYTRLSRLNYSLDNGMYPLGSCTMKYNPKICEKAARISAFANLHPLQPIE
                     SVQGAILILYEMQIILQEISGFPACSLTPAAGAQGEFGGVKMIAASLRDRGEMQRKIM
                     LIPDSAHGTNPASSVMSGFECLAVRTDERGNIDLNDLTSKCNEQLAGIMITNPNTLGL
                     FDENIVEVIRIVHQAGGLVYGDGANMNALLGIVKPAELGFDVMHFNLHKTFATPHGGG
                     GPGAGAICCTEQLKAYLPGPIADILEEATEEVPPLYGYRMPEKSIGMLRSFWGHFGII
                     VRAYCYCMMLGAEGFRQVAEYATLNANYLMEALKQDYPLPFDRTCMHEFVLDVKWPDA
                     PGIRALDVAKRLMDYKFHPPTNYFPLIVHEALMIEPTETESKETLDDFIKAMKAIAKE
                     ARENPELLHRAPAETPFGRMDEVKAARDLKIAE"
     gene            complement(15367..16728)
                     /gene="gcvPA"
                     /locus_tag="PK898_05440"
     CDS             complement(15367..16728)
                     /gene="gcvPA"
                     /locus_tag="PK898_05440"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOU19512.1"
                     /translation="MFIPHTDADRESMLAAIGKKDLNELFDVVPEKYRFPKLNLQPGL
                     TEPEVTFLLDEISSANLSLDHTACFTGAGAYHHYIPAAIDNLISRGDFYTAYTPYQPE
                     ISQGTLQAVYEYQSLMARLTGMEASNASHYDGATACAEAGILAYHQYHGKRKKILVSA
                     GLHPHYLLTLKTYLQDFDGMQIECPKAASLDEINPQRLEEKIDTDTALVLVQYPDFFG
                     QIQDFSELAKAVHEKGSLLCVCINPIALGLLKTPGEMGADIVVGEGQPLGIPLSYGGP
                     YLGIFTTRKELIRKVSGRIVGETVDNRGQRAYVLTLTAREQHIRREKATSNICSNQGL
                     NALTAAIYLSLLGRNGLQAVAKLCYDKAHYAAKEISKIHGYRILNQRPFFHEFVVSCP
                     QAPSDINQHLLDHGMIGGYDLSEEYPMLGNAMLIAVTEMINKEDIDDLCELLKEGEHH
                     GRH"
     gene            complement(16732..17121)
                     /gene="gcvH"
                     /locus_tag="PK898_05445"
     CDS             complement(16732..17121)
                     /gene="gcvH"
                     /locus_tag="PK898_05445"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOU19513.1"
                     /translation="MNIHPNYFYSKNDEWVSVEGNKATIGISDYAQDSLSDIVFVEIS
                     VSAGDSLAAEDNIGTIESVKAASDIYTPVAGTVTEINNKVIDSPELLNRDPFGEAWLI
                     KLSSDTGFDTSHLMDADAYRKYCEDRG"
     gene            17273..17497
                     /locus_tag="PK898_05450"
     CDS             17273..17497
                     /locus_tag="PK898_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19514.1"
                     /translation="MSIKLLDNSLELEIFLDKEDSEFEDNICLRFTEDCPEDEKVFYG
                     GETNIYITRDQALQIVKALGAVLEEANESN"
     gene            complement(17550..18620)
                     /locus_tag="PK898_05455"
     CDS             complement(17550..18620)
                     /locus_tag="PK898_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HOU19515.1"
                     /translation="MTFDESLSSERSQLSAFFDEGVRNRRMLSQAAFQLSDIASSKGW
                     KFICYCRQVKAIFSNSEVHFLPKIRNFLTATAKIVGLNHSDIFRPGPTSSSPVSPEEL
                     QIRLFGEKIEKNSEIAVVLHLFYVGLWPEIAELLSDIPEPFDLFISLPKEQKNFRSEI
                     QKYFPNAHIYIAPNRGRDIAPFLEIFRAIFPLDYQFLCKLHSKRSTHLSTGENRRQSL
                     FSELIGSEQRVNEILQLFRQNIEIDMVVPKGFLTGGIDIDTFTNRKNLETLAGLLNIP
                     LHDYYFEYPAGSMFWCRPGCLEKLAGSGIRTADFPPERGQLNNTLAHAVERIFGLLVT
                     DEGHQLIDTSVFQSYLNRKEKH"
     gene            complement(18671..19786)
                     /locus_tag="PK898_05460"
     CDS             complement(18671..19786)
                     /locus_tag="PK898_05460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU19516.1"
                     /translation="MNILFVGNCTPYFSSGIVRAKRFFPSLSEAGISYDWMEINAAAV
                     QRWLERLDRSIFGKQKITDLIFRSLIHGLGLPYYWVQVLRILRQQSRYDVIFFQSVLL
                     PPRAMRYLAKQNSKIIFDYDDALYVKHESKTRGMIQYAWKVFAGSHALYDFAKAINLS
                     TILLPSSIPVEKYSVEATQEPHSPLRIGWIGGPSTLNQLEIVVDPLKNMLASGIDFEL
                     LIAGSKNQPIPLSKIEKLHILEIPEYSDDDIPEIVKKMDIGIMPLFDTPAEQGKCAMK
                     MLIYMAGELPVVASPVGESFYIVREGQNGFLAETAAEWEAKLSRLIGDADLRKKIGKA
                     GKQTVTERYSQDIIFRKMISEIFPDESIKNEPYANRT"
     gene            20060..21037
                     /gene="mvaD"
                     /locus_tag="PK898_05465"
     CDS             20060..21037
                     /gene="mvaD"
                     /locus_tag="PK898_05465"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOU19517.1"
                     /translation="MSDSVATAVAHPNIAFIKYWGNRDDSLRLPANGSISMNLGALET
                     RTTVAFSSAYRSDRVSVNGSLSEGESLIRVVHFMDRIRSLAGEERFAEIESRNNFPIG
                     AGIASSAAAFAALALAGSAAAGLHLNEAELSRLARLGSGSACRSIPPGFCEWLAGDSD
                     EESIAVSIAPPDYWALTDIIAVVSARHKEVGSSIGHQRAMTSPYQAVRVATAPERLLE
                     CRTAILKRDFDRLALICEKDSTMMHGVMMTQEPPLFYWEPASLKIMKNIMQWRREGIP
                     CFFTLDAGPNVHVLCPEEVEKELKERLGKLSEVENLLSSGPGDAAHLAD"
     gene            21027..22004
                     /locus_tag="PK898_05470"
     CDS             21027..22004
                     /locus_tag="PK898_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU19518.1"
                     /translation="MRIDPNENGLLNPMDQIINLILPILIVVLAGYCAARFHVLDKGT
                     GNVLCQYLFYFSLPFLTFTNIYRAGLDNLLNGRFILTAILTIFGIILLAYFVFRFLFR
                     SEGQNLIMMIFGSFYVNATYVGVPVASMILSSVVPPLIILMIQASFFFPLTVFLMDVN
                     SGKQSRINFKDSLLLLVRNPILVATLIAIVLLVFQIRVPQFVLTTTELFGKPAAATGL
                     FALGFTCYLPADQKITKSEIRRALTVTLFKTFLQPLLAWSIGRFVFHLDSWWLTAIVV
                     CAMLPTAVNHFIVSQKYRCSENESKLIILFTTFGFTIMVSLFLYFSAGF"
     gene            22081..23847
                     /gene="mutL"
                     /locus_tag="PK898_05475"
     CDS             22081..23847
                     /gene="mutL"
                     /locus_tag="PK898_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU19519.1"
                     /translation="MTIKILADDVISKIAAGEVIERPASVVKELIENAIDAGANEISI
                     RVEDSGKKLIEVSDNGCGMNAEDLRNAIQRHATSKLRDADDLFHIHTLGFRGEALAAA
                     GSVSRMTIRSRMASDLNGQKITVDSGNILGLKQESMPAGTIVSVENLFCNVPARLKFL
                     KSDTTEKNQIDTLVSRYAMAYPSIHWQYTHNQRPVLATSGNGKAREVLASIYGIDVAR
                     QLLDVDLEEDNIHIRGFISPVALTRSNRREMIFFVNGRWIQDIQLNSAVMRAYQSLIM
                     VGRYPIVILSIEMDPEEIDVNVHPSKAEIRFRSADKVISAVHRSVRFGLVSQSPVPEL
                     SKPIQWQTWAENRSLTPNVSSAVSHPADEEENTDIGFPQADPNSRTFRQENFVSPAGA
                     ASHFPILRWIGQIGGTYILAEGPDGLYLIDQHAAHERVLFEELLNRPRDESISQILLD
                     PEVVQLPPWQADLLEKNIPILEKLGFEIEPFGPSAFRIIAIPSIFKKGNPAAAIRSVV
                     EDFEEDEEPLKAEIENLIAARVCKSMAVKGGQILSDEEQRALIRDLENCQNPRTCPHG
                     RPTMIHLSINLLEQQFGRKGKI"
     gene            23923..24789
                     /locus_tag="PK898_05480"
     CDS             23923..24789
                     /locus_tag="PK898_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU19520.1"
                     /translation="MMMQIVTDSGCDLSDEQKRGLPIHVVPLGLVLNGVSYDKNHPIT
                     PAEFYQKMVETDVYPSTSQASAGDFADVYRELSANGEEILSIHISSGLSGTLNSAKVG
                     GALVPNAKITYWDSKTLSSGLGWQVQAAALAIKKGWSLDKILERLALIRDQVDGMFTL
                     KEMRYLIHGGRVSHLEGLMAQVLNVKPIIAVEHEKGTYVTAGKLINFKRALAQIVEIN
                     YEKFKNEKVRAQIVHGINPEGVEYLKEKLQEKLDVVFEEVLPVSPVLGAHTGPTLTGL
                     IIGPMSFFADLI"
     gene            24881..>25602
                     /gene="tilS"
                     /locus_tag="PK898_05485"
     CDS             24881..>25602
                     /gene="tilS"
                     /locus_tag="PK898_05485"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU19521.1"
                     /translation="MKPEPFDRTRFLEFLEKECGILPGDAVLVGVSGGADSLFLLWHL
                     HENGFRTTAATFNHQLRPSAADEAEFVRRTCIEWSVPFLGGTQDVMSYARQRNLSTEE
                     AARECRYRFLFAAAEKIGAAAVATGHHADDQAETVLMHFIRGSGLDGLAGMRPRTFFP
                     QWSKTIPLVRPILPISRTEIESWCLEKGLQPVSDESNMDNNYFRNRLRNRLIPLLEAE
                     YNPQIRKNINKTARVIQTDLDW"
BASE COUNT         6558 a         5823 c         5989 g         7232 t
ORIGIN      
        1 cgtccttgcg aagcacgccg aggtacgagg tgcgtacgtc ccgcgccgaa ctctttcctc
       61 accctgaccc tctcccaacg ggcgagggaa aaaacggacg gcttcctcga cgtcccgtgt
      121 tcataaaaaa tattgtaggg gtcggttcca aaccggccct ttttaataac tatgctcgaa
      181 agaacatcca taggggacgg cgtccttgcg aagcacgccg aggtacgagg tgcgtacgtc
      241 ccgttccgaa ttcttccctc accctgaccc tctcctaacg ggcgagggga aaaacggacg
      301 tgtcccgtat cgaatccttc cctcaccctg gccctctccc aatgggcgag ggaaaaaacg
      361 gacgacgtcc cgcgccgaat ccttccctca ccctgaccct ctcccaacgg gcgagggaaa
      421 agcggacacg tcccgcgccg aatccttccc tcaccctgac cctctcccga cgggcgagga
      481 gaaagcagac acgcctctat cgaatcatcc ctcaccctga ccctctccca acgggcgagg
      541 gaaaagcgga cacgtcccgt ttcgaactct tccctcaccc tgaccctctc ccaacgggcg
      601 agggaaatat aaactgccgg gggatccatc cgtttatggg aggcatgcta taattcgtcg
      661 atatgaaaaa aagaatatat attttgattc aaatccttgt tattctcttc gcaaaggtag
      721 gtttcgtcgg cgcacaggat caaaaattga acatccttac gacgattttt ccgatttatg
      781 atttcacccg gacagttgcc ggtgactttg ccgaagtttc tatgctgctg ccaccgggcg
      841 ccgacagcca ttcttatgag ccgactccgc gggatatcct gaaaatccaa aacgcggatt
      901 tgttcattta tatcggcgga gaaagtgata tctgggtcga tcaaatcctt tcctcgttgg
      961 gtgataaaaa accgcgaaca ctggtgttga tccaatccgt gaacgcttta ccggaagaag
     1021 tcgtcccggg aatgcaggcg gaagaggaat cccaatcatc cgatgaatcc gatgtggtat
     1081 atgatgaaca tatctggctt tctccgctga acgcaatcaa gatggcggat gccatagctg
     1141 cgtcattaac cgaatcggat cccgctcatg cgcctgattt tgagagtcat cgcgcggcac
     1201 tcgatcaaaa attacaagag ctggatcaaa cttttcggga cattgtcaaa tccgctaagc
     1261 gtaagaccat tattgtcggc gatcgattcc ctttccgtta tctgacggaa gaatacggat
     1321 tggaatatta tgcggctttt cccggctgca gtgcggaagt cgaacccagc gcttcgaccg
     1381 tggcatttct cattgacaag gtaaaaacgg aaaaaattcc ggttgttttt tatatcgaat
     1441 tttccacgca taagatcgcc gatgcgattg cggatgcgac cggagccaaa accatgctgc
     1501 tccattccgc tcataatgtc tcgcaggagg atatcgattc aggaattacc tatctcagca
     1561 ttatgaccga aaatgcggaa aagctgcgtg aggcgttaaa ctaagctcag ccctgccggt
     1621 acaggtttca gcttttcact tcagaacagg atcggtatac gatgagtcaa ctgattcatt
     1681 ccatccaaaa tttatttctt gatgatctgg gaaaaaccaa tttattggga aagatcatct
     1741 tggcgattct tatttatgcc gtgatgaaga tcgttctttt gatcatcaat aaagctctca
     1801 gacgtttttc atcccataaa tttccgaagg taccggaaag caaaaagatc accgtttttt
     1861 cggctttgaa cagcttcgtc aaattcatta tttatttcat cgcaattact tttattttag
     1921 atttgttcgg tatcaatacc agttcgatta ttgcgacagc cgggatcggt ggcatcgcca
     1981 ttgcattggg tgcccaaaca ttggtgaagg atatcctgag cggactcttt attcttttgg
     2041 aggatcagtt caacatcggt gattacctga ccatcggatc ggtcaccggt acggtggaag
     2101 aaatcgggtt aagaaaaatc aaggtgcggg atgacgacgg atcgattact ttgatcccca
     2161 acagcctggt gcaacaagtg acgaattatt ccacccatcc gatcaaaatt aatgttaaat
     2221 tcccggtccc gtatgccata aaaccggacg aaatcggcgc catgattgaa aaaatatccg
     2281 aagaatgccg aaaagaggtt gactatttca cggctgcccc cgctttttcc gggatcgaca
     2341 gcttagcgga ttcaaattac acggtacaag ttgctgcgga aacgatatcg gggaatcagg
     2401 gcgccggaca gcaattgatc aagcagaaaa tattaaaaga aattcaggat cacggtattt
     2461 tttcagccgc cgtcgacgaa aattccgaaa aagctcaaca gccttgaagt taaggacgga
     2521 tatcggcagt tcccaaaggc agccggcgat gcgaaccaaa attgtttgtt cccaaaagga
     2581 gaaaaaatga aagtaatttt tcatgtagat gaaccggaaa aatggcaact cgctttggaa
     2641 aatgttcgga attttttaaa tgccgaaccg gaagcggaaa ttgaggtact cgcgaattcc
     2701 gccggtgtca aattttatat tcaagaggcg tcgacaaatg atttgttcgt ggaattggtc
     2761 ggagaaggag tcctttttgc ggcttgtaac aatgcattgc ggaagttcgg atttcggaaa
     2821 gaggaactgc cggaagcggt tatcatcgtt ccggcgggag tggtcgaact ggctgaacgt
     2881 caggaaaaag gattcgctta tattaaacct tgagccggag aaatttccgg acggttccgg
     2941 gtttgttagc tgcgccggaa gagtttttga attcaccgag acggccgtta gggttctccg
     3001 gtatctttct atgattccga aaggtgtatc agaaaatctt cttgaaatcg ttatttggta
     3061 aaatgggtaa tattcattaa atttgaaaat tgggagaaaa aatgaaaatc aattatcagg
     3121 agacgaccaa cgatctgttg accaggatcg atatccataa taaatacggc agtaaagata
     3181 ttgatcaatg gatgttggat acactggatc tgaaacccgg aataaagatt ttggatgtgg
     3241 cttgcggcgg gggcaagcaa tgtatttcgt tttataaata tttaaagggt gactgtgata
     3301 tcaccggcgg tgacgtgaac caagaactgt tggatcaagc cgagctggaa aatgtaaaac
     3361 tgggcagccc tttcaaagtg attccgttgg attttgataa acggtttcct tttgacgatc
     3421 aaacttttga tctggtttcc tgttgcttcg cgatttatta tgcgagtgac attcctttta
     3481 ccattcggga aatgcaccgt gttctgaaac cgggaggtcg aatttttacc accggaccga
     3541 tgccggacaa caagaaagtg ttctacgaca ttattcgtga ggcaaccggt aaagaaattc
     3601 ctcccatgcc cggaagttcc cgctacagca ccgagattta tgatgccatc gcacagacat
     3661 tttcgaaagt cgaaagtcat atctttgaaa accctctgat tttcaagcag ccgggtccgt
     3721 tcatcgcata tacgcgtgcc agcttatcgg aagatcgccg gttatggaca tccctgtttg
     3781 aaaattcgga tgaatttgag aaaatgatgc aaaagatctt gaaagtcgcc gacgaacgaa
     3841 tcaaacggga cggagaattg gtgatgacca aagtggtcgg cggttttatc gggaccaaat
     3901 aatatgagcg aaatgagatt ttacggcaag cggattctct ttatcggcgc ccatccggat
     3961 gatatcgaat tgggttgcgg tgcgttcatc gcccaaatcg cctctcagac ggaggtacgc
     4021 tgtgtcacac tttccgataa tcaaaaagat ccggcactga agaatctgcc cgatgaacat
     4081 ttcgccgcta tggcagcgct gggagtaaag ccggatcggg tatcgttgtt ggcttatgaa
     4141 acccgccggt ttcctttttt tcggcaggaa attctggaag agatggtcaa gttgcaacga
     4201 gagtttcagc cggatattgt gtttgtccat acttccgccg atgttcatca ggatcataaa
     4261 acggtaacgg aagaggcact gcgggctttt cgaggaagaa cggttttggg ttttgatgtg
     4321 attcgcagca gctacggctt tttcccgagt tttctggttt ccgtaaccga agaacaggtt
     4381 aaccgcaaga ttgaatcgct ccgatgttat caaacttatc aatccaaata ttattttgac
     4441 ccggagatca cccgcgcgac actgattcgg aacggggcga tttgtgagcg aaagtatgcc
     4501 gaaggatttg atatattgcg tattgtcgct tcgtttggaa aagattgaca tttatggctt
     4561 ggaaaaatat aaagaaaaca acccttacaa atttttggaa aattgttacg gttgtttttt
     4621 ttattttgtt gttgggaatc ggtatcgtta ttcacgatga ttacggagga tataccgacg
     4681 aattaattga gattcaaacc gggatgatcc aacttaaata tgtcttctct aagatcccca
     4741 tgttggaaga tatctccgga ttgaatatcc cgttgtacgg aaattttgcc gcacttcccg
     4801 atctggcaac gtatgaagat cgaatctatg gcagtgctgc aatgttaccg actttttttg
     4861 tcaattactt gcccggaatc tcattggatg tcggtgaatt tttgaatttt cggcgctttt
     4921 ataatttttt aaattttttt ctggctgcca ttctgtttta cctttttttg caaaaacgtt
     4981 tccggaatcc gttaattgct ttgaccggga cgcttatgct cgttctttct ccgcgaatat
     5041 ttgcggaatc tttttataac tgcaaggata ttctattttt aagttggttt ctgatcagtt
     5101 tattgggaat cggtttttat caactggatc ggaaaaaata cggcttagtc ctgtttgggt
     5161 ttgcgttcgg attggctgcc aatacgcgat tttacggatt tattcttctg ccggcatttg
     5221 ccggaatcgc aattttcgat atgattacgg atccgaaaaa ccgaaaaaaa atacttcggc
     5281 aaacttttct gacctgcatt gcatctatcg tttggttttt tattttgaca ccgtttttat
     5341 gggaatcgga ttttcaacaa attattggcg gattgaaagt agcggtcgat cagcctgtaa
     5401 tcggggatgc cgagttattc atgggaagat ttgtcgctcc gcgcgatgtc tggtattatt
     5461 tgccggtctg gatcggaatt accgttccgg tgctttattt ggcgcttttt gtgatcggat
     5521 cggcatttct gccattcaaa attcaatcgc tgaaagactc cgatactcgg tttgagcttg
     5581 catttgacat aatgaacttt ttgctttttg tgatagcgat catggcgatt gttatttcaa
     5641 aaggcaccat ttatcatggc tggcggcatg cttatttcct ttatgcgccg ttcatttata
     5701 tctctgtgtg cggattaaac gatctgattg aattcaaaat ctccggcaaa acaaaaatga
     5761 aaatcaaaaa cggaatcgtg ttttcgcttt gcctgctttc ctttgccaat acaggatggt
     5821 ggatgataaa aaatcatccg ctggattttg tctattttaa tgagatcgga aagaattatg
     5881 cggaagattt cacgcgagat tattggggtg tagcgtccaa aagctgtcta ttatatttat
     5941 acaatgcggt tcaggtgggt gagatacggg tcggggtaaa tagtgattta acttacggat
     6001 cggctgaaat taccttattg cgtttgccgg aagcggttcg aaagcgcttt acaccggtct
     6061 ggcagatgaa aaatgccgat tatttatgtt acagttataa aaatacaccg gggaacgatt
     6121 accaaatccc ggattttgcc gtgatgaaga cctttatggt ggacggatat gcggtgatgg
     6181 gagtttatca acggataaat cccgccgagt aagaaagagg gggctttttc aaaaagcccc
     6241 ctctttcttc cgattagttt ttcggataaa tgaccacttt tcgattgggt tcttccccga
     6301 cgctttcggt ataaatatca tttcgatctc gcaatgcgag atgaataatt cttctttccg
     6361 ttccgggcat cggttcgaga gaaattcttc ggccggagcg agtaacttga tccgccatgt
     6421 gaatagccat ttgtcgtaat tgacgttccc gtcgttctcg gtatccttgc acgtcgatca
     6481 tcatcggtac ccaatggccc aattcacgtc cgacaattaa acttgttata tattggaatg
     6541 cattcaatgt ctcggaatgg cgaccgatca gataagataa atcatcccct ttgatatcga
     6601 caagaatcat ttcctgattg ccggatgttt caagttttcc gatccgtccg gtcacacttg
     6661 ccttgatatg cattttgtct attaagtctt cgacgaccgt cacagtggct ttcaatgcgg
     6721 tttcatcaac caactcttcg ccggcggtct tctcttcctc attttcggat tccgcttcgg
     6781 ttgtatccgc ttcattcaca tcgccgggaa gctcttcgct gatttctttt gccgatattt
     6841 tatcggattc cggtttgacg gttcggccgg tttgagaaga ttccgattgc ggagctttgg
     6901 ctgccgattc cttcaccttc aagcttacgg aagccggcct tgagccgatt ccaaagatcc
     6961 cttttgctcc cgaatcgatc acaacaatat cgacctcatc ccgtgttttt cccagagctt
     7021 ttagcccttt ttcaattgct tcatccacag tccctgctga aaattccatt tgattgtcca
     7081 tagtctatat tgccttatct actctgtcgt cactttttct tcggtttttt cttgtttttg
     7141 aactcacgat aatcaatctt ctcttccgcg ggttctatcc ccgattccgc taaattgggc
     7201 gtcatcgatt ttttgggatt ggcactggca acgggacggg aagttgttac cggtttttcg
     7261 gccggtgtag ctaatttcgg catttgcggt ttaccgaact ttaaggcttt ccaatttaat
     7321 tttccttcca caccatattg tacgatcgta aaaatgtttg aaatcaagaa atagagcgaa
     7381 agaccggagg ctaatgtgta agccatgaaa cccatcagaa gcggcatata aatattcatc
     7441 ataccggtca tcatggctcc gctggctgcg ttcttgtcat tcggattggt cggcgggacg
     7501 gtcagtttgg tttgcataat ggtcgtgatc acaaccagaa tcgccaaaac cggtatgccg
     7561 aaagagatcc cctgaatgta caatctttca ggctgcccca aattcatcca caagaaattc
     7621 ggattgatcg ggaatatttc accgatttgt aaaaagccgg aaaaagcgcg ttttgtcaaa
     7681 tccaacattc ccaacggagt cgatacgatt gccgcgataa tggcttgata taatgcgaaa
     7741 ataatcggaa gctgtatcag tgtcggcagg caggaagcgg taggatttac tccgaacttt
     7801 ttataaagtt tcatttgctc ttctgccagt ttctcctgat ttcctttgta cttctccatc
     7861 atttcgaggt attcgggaga tttttgcagc tccatcattt tggttgagct ttggatctgc
     7921 tttcgcatca acggataggt gatcagttta atcaggaccg taaataagat gatcgcgacc
     7981 ccaaaattcc cgaccagttt atagatatag agcaaaatat tcagaaagat atttacaaaa
     8041 gattgccaca tagtcattcc ctcctatttt gccggtgttt ctgtgacggt ttcttggctg
     8101 acttccgcgg tcggtgtaag tgcggtcgat tcgactgttt cgatatacca ccaatcttct
     8161 ttcagatccg atgtgtaact tctcaagagt gcatctccgg ggatcaaacc ggcgacaaat
     8221 gattcaccga taacaaccgg cgagctcatt gttttcccat taatcagacg actgtttacc
     8281 gatttcccgt ccgtatcata aaccatcagg ttgccgtctt ccgcgaccgc cagaagattc
     8341 ccgttttgga ggaggatcgg atctgcgata aatcccactt tgtcggtgga aacggcttta
     8401 atctgccata tagatttccc ggattccgac tcattgcaat aaaggtatcc ggacatgtcc
     8461 gcgacgataa tcttgccatc gatgatcagc ggcgatgccc aaatttcccc gttggtgtgc
     8521 atcaccgttt tgatttcttc cgtagccgga tcaaaataag ccacttcttt tcctaacgtt
     8581 ccgaaataga tccgatcatt cgcgaatacc aatttcccca tcacagcgcc gcttatggac
     8641 agtgctttct cgattgttcc ggttttttca tccaaaacca ggaatcgttt atcgagtgtt
     8701 gtgacataga cctttccgtc tgacacgaat ggactgaccc atatttcggt ggaaaggttc
     8761 gtttgccaga cgggaacgct taagttatcc aaagtgtaag cgtagaccga accattattt
     8821 gaagcgatga aaagcatgtc gtctgcgatt accggagagg caaggatttt attcttgttg
     8881 gtctccaaag tgatcttggt aatcagcgtt ccgtcatttt gatccaaaat atgaacttcg
     8941 ttttgatatg ttccggtata aattttcccg tcataaatcg taggggcggc aaaaaacatc
     9001 atcccgtttt gggcttccgc cggatatttc cagagcaatt ttccgttaga aacggcttga
     9061 acggaagaag tgtatgcgac ataaaccgtc tcgtcatcgg ctgacaatcc cggccagctg
     9121 accgcattgc tcatcgccga atttccggca catccgctga gaatcattgt tgtgaggatc
     9181 atcagcaata ccagtaaaaa ttgttttttt ctcttcattg aatttcctta tcggaggatt
     9241 ctttacggaa ccgggtcaaa tccgccgggg ttgaacgggt tgcaacgcag aacacgccaa
     9301 attgccatcg gtacgccttt gaatgctccg tatttataaa tcgcttgata agcataatga
     9361 gagcaggaag ggtaaaatcg gcaggtgttt gcgggcagca actttgatat tgtattttga
     9421 taaatacgaa ttaatatcaa aaagaaaaaa cggggcaaat ttgaaatgcg aaacggaagc
     9481 tcgctcagac gaggttcgcc ggcataagca tcctgtgaac aatcgctttc tttccttctg
     9541 aaaagagagg aaattgcccg aatcatttta tctttttgtg cgatgtgaat cattttattg
     9601 cgaagaaggc agcagaactt cggctttttt caacaaactg taaaccgcct tggaaacgtt
     9661 ttcgatatcg gcttctaaaa tcggtttgcg ggctatcaaa acgagatgat agttgggttt
     9721 aatttgatcc gtataaggtt cgatggccgc ccgaatcagg cgttttgacc gattccttga
     9781 tacggctcct cccacggatt tggtggcaat aataccgatt ttcgattcac cgatgttcgg
     9841 atccattaat gtggttaata ccaccatagg gtgagaaaaa gatttccctc tttgtctcac
     9901 cctttggata tcatctgatc gattcagacg ctttttacgt ttcaatttag caaacgctcc
     9961 acttatacgt ggatagttct gttcaaaaat ttgttgcgaa aagtcatgct tctgaaatac
    10021 taccagcgaa tacgctttac atgattattc atcgaaacgg tcaaattatg acgaccgcgg
    10081 gctcgacgtc tcttcagaac atctcgaccg ctcgctgttt ccatccgttc acggaaacca
    10141 tgcacacgaa ggcgacgtcg ttttttgggt tggtaagttc gtttggttga catttacttt
    10201 cctttcaatt ttgaataacg gcagagatcg gcgtatgggt tcccccgggg ttcagttcag
    10261 gcttttcaaa aaatttatcc taaacgcttt gaaaaaccgg cacattccgg catgccacca
    10321 ctcctgccat catatttttt ctttaatagc caaagaatta tatcttaatc catgaggacg
    10381 gtcaaacatt tggtacataa ggtctgcgag agcgttttgg aaaccgaaaa gaggaagatg
    10441 tttccagata agagattcat tctctgcggt actccggaaa ctcgagacat taaaaaaaat
    10501 tccggatggg aattcccatc cggaaaatga tgattgatct tgacggacag tgatcagtaa
    10561 cagcgccaat tcactctgtc cgagaataaa ctgccggtca tatataatcc cagatccgag
    10621 aaattcaccg ccacattggc tgctccgtca ctgttggtct gactgatcag gttggtgagc
    10681 cagattctgc cgaccaaagt tacgatttcg cccgggccga gagcgacctt atttccgtgt
    10741 ggagcgacgg aagagagcca gtaaaaggcg atcggagctc caccgttgac agctacggaa
    10801 cccggcaggt taataaagcc ggtctttgcc ccgtcattgc gcagtcgaat ttgcatgacc
    10861 gcaaatttcc cgcatttttg atataagccg acgaccgctt tatcataagt gttataccaa
    10921 ccggaaggtt cgatcggatc attcgcatct tccatcgaat ccaacggttt caacgggaca
    10981 atcttcgcat cgcataattc ggcgggaccc ggaacgcttt ccataactcc gctgatcata
    11041 tccgggttat acccgaagcg ccaattcaca ttcaaatcgg caccgccgga agtctcggca
    11101 accggattgg tcagatcgtt gatgatcgct tcgaaacgga tcttgtcccc cggttttacc
    11161 ggaatggctt tgcccggtgt gcagtaatcg ccgccgaaag gataaccttc ggaaccgaag
    11221 cgtgtatatc ggcaggtgta attcgagtag gaagtccctt cgacatagat ttgtgcggga
    11281 acaaggcatt cgggtccgtc ctcatccggc acttcgatat cggctttgaa agaggctttc
    11341 ccgccgcatt gttcataaat gcctttggtc gcaaccacgt ccacgccttc tgtcgcgcat
    11401 aaatcttttg ttgatgtagc gcttcctccc gatttggtaa ctggagtttc caatgctccg
    11461 tgagcagttt ttgaatagtt aatctcgaca tcaaaagaaa ccggagtggt atctaaaact
    11521 cttggaattt caacatagcc agttaaagtt gcgttcccgc tcgcatccaa atagactttg
    11581 ctgcaggcat ccccatattg agatttacag ttctgtaatt cgtaatcggt ggtaccaatc
    11641 gtcacaataa ccgatttcac aaaaatcata ttgggcattc cggtcgagct gctgtcagcg
    11701 gttcgtttta gaccgatttc aattttgatt tttccgtctc cgcaagcgac aaattcaccg
    11761 gaaagtgtga ctgttgccgg atcaatgatc gattttgcct gtaaatcgac gcctttttcg
    11821 ccttcaacag ttacttcgtc cgttaattca gccgtcggaa ccggattctc aacacttttg
    11881 gcttcgaatt cagtcaaatc gactgcttcc gaatccacac cggctaaagc gagatcttgg
    11941 gtcgtccagt cttcaattgc gccaaaataa ccggagactt catcctcgct taattcctgt
    12001 gcaaatacgg ggaatatgga tatgctcagg agtaatacca gagccaataa gatggaaagt
    12061 gtttgttttt tcattagatt atgacctcat ttctttcgaa ataataattt cttatttccg
    12121 tcccccaata atttgggttt gatttctatg ttgcaatatc aatgccattt atcgattttc
    12181 ctttattttc ccgaaaactt ttcgggaaat atttaaccga tgtcttctat gcgtgcagga
    12241 atcgcgccaa aatgaaggat tttgaaaaat gtctgaaaat ctcttcagag agggacaatt
    12301 gattcgatca actgagtaaa aaaagaagaa gcggggaatc aattcacccg cctcctcttt
    12361 tttaccgcta aatgattttt ttactgattt cgattcgata accaatcgat caacagacga
    12421 accccaaagc cggtggcacc ggggacggaa taatcggttc cttcatcaag gtcagcgacg
    12481 ccggctatat ccaaatgcgc ccatgggacc tcatcggaaa caaattcttt caggaacatt
    12541 cccccggtta cgggagaagc cagacgaccg cccgagtttt taatatccgc atcttttcct
    12601 ttaatcaact tggcataatc ttcatcattg ggcagttgcc aaatgcgttc ccatgtcctt
    12661 tcccccgatt caaacaattc tcgataaagg gattgattat ttgtgagcac tccggctcgt
    12721 acatgcccgt aagcgacggc acatccgccg gtaagggttg ccaggtcgat gatggcttgc
    12781 gggctgaaat tttgttgggc atatgttaat ccgtctgcca ataccaaccg cccttccgcg
    12841 tcggtcgaga tcacctcaat acttttaccg gagagactga tcagaatatc atccggacga
    12901 taagaatttt ggctcagcat attctctgcg gcgcagatga ttccgataat cggggtcgcg
    12961 attttcaact gagaagcggc tctcaaaact cccaggacag ccatcgctcc gcacttatca
    13021 tatttcatcc cgaccatgct gtcgccgggt ttaatcgaat agccacccga atcaaatgta
    13081 agcgctttgc cgactaatac gaccggtttt tctttttccg ttcctttata ttcgagaatg
    13141 atcatgcggg aaggcgtttt acttccctga ccgaccgcta cgatggcgcc ggctcccatt
    13201 tcggttaatt gcttgtcatc caaaattttg caattcaagc cgaattgttt tgcaaatttt
    13261 tgtgctcgtt cggaaagtgt ctgaggatta atgatcgcag ccggttcatg tgcccattcc
    13321 cgtgccatat tcaccgcatc acaaaccgtc ttacatgttt gcaacaatat ttcttccgaa
    13381 ggatcaagat aaatgacgga atcgacggga tcctcttttt tcttgtactt gtcaaaggaa
    13441 aatgaaccca gcaacaaccc ttccaacaaa gctttatgat ctttgtcatt cagatcggcg
    13501 gggatttgaa gcgccgcggt tttgatgccg ttcttcttga tccatttcgc gatgattcct
    13561 ccaagggagc ggtatgtatc ggcattgatt ttttcaccgg tgtagaaaga aacggttgtt
    13621 tttgcttttt ccttttcgac aaccgaatcc cctttttcct ttaataattc actttttgct
    13681 ccgctgacga tctgcagccg gatcgctccg gcgggaattt ttggggatgt tttaatctca
    13741 atatcggttt tcgtgtcaat tatgtctctc atttttcctc tttatatcat atcggttaat
    13801 tgattacaaa tcgtcattcc gcggggaacc tttcgatcgg atgggtaagg tttttgcccg
    13861 ttttcaaaaa aaatcccctc cttctttcga aaaggcccgc ggaatgacaa cggtttgttt
    13921 ttattccatt actcggctat tttcaaatct ctggctgctt tcacttcgtc cattcgtccg
    13981 aagggcgttt ccgccggagc ccgatgcaat aattcgggat tttcacgtgc ctctttggca
    14041 atcgctttca tggcttttat gaaatcatcc agcgtctctt tgctttcggt ttctgtcggt
    14101 tcgatcatca aagcctcgtg aacgattaat gggaaatagt ttgtcggagg atgaaatttg
    14161 tagtccatca gacgtttggc aacatccaat gcccgtattc cgggtgcatc cggccatttt
    14221 acatccagaa caaattcatg catgcaggtg cgatcaaacg gcaacgggta atcctgtttc
    14281 aatgcttcca tcaggtaatt ggcgtttaaa gtagcatatt cggcgacctg tcgaaaacct
    14341 tccgccccca acatcataca atagcaataa gcccggacaa taattccgaa atgaccccag
    14401 aaagatcgca gcattccgat acttttttcg ggcatacggt atccgtaaag cgggggtact
    14461 tcttcggtcg cttcttccaa aatatccgcg atcggacccg gcagatatgc cttcaactgc
    14521 tccgtacagc agatggcacc ggcgcccggt ccgccgccgc cgtgcggggt ggcaaaagtt
    14581 ttatgcagat taaaatgcat gacgtcgaat cccagttcgg ccggtttaac gatccccaac
    14641 aaggcattca tattggcgcc gtcaccgtaa accagtccgc cggcttgatg aacgatacgg
    14701 atcacttcga caatattttc atcaaacaat ccgagggtat tgggattggt aatcatgatt
    14761 ccggccagct gctcgttgca tttggatgtg agatcattca aatcgatatt gccgcgttca
    14821 tcggtacgga ccgcaaggca ttcaaagccg ctcatgacgg acgaagccgg attggtcccg
    14881 tgtgcgctgt ccgggattaa catgatcttt ctttgcattt ccccgcggtc gcggagcgaa
    14941 gcggcaatca ttttcacgcc gccgaattca ccctgtgcac cggcggccgg cgtgagcgaa
    15001 caagccggga atccgctgat ttcctgtaaa atgatttgca tttcataaag aatcaggatc
    15061 gctccctgta ccgattcgat cggttgtaaa gggtgcagat tggcaaatgc ggatattcgg
    15121 gcagctttct cgcagatttt cggattgtat ttcatggtgc aggaacccag cggatacatt
    15181 ccgttgtcca aagaatagtt aaggcgggat aaacgcgtgt aatgacgaac gacgtctacc
    15241 tcggaaagtt ccggcagcgg caactctttt cgcagtaaag actccggcaa atcacttgcc
    15301 ggcacatccg atgaggggaa gcgaaaacct tttctgccgt tcgccgaaag ttcaaaaatc
    15361 gttttctcag tggcgtccat gatgctctcc ttccttcagc agttcgcaga gatcatcaat
    15421 atcttcctta ttgatcattt ccgtcaccgc aatcagcatg gcattcccca gcatcggata
    15481 ttcttcggag agatcatacc cgccgatcat tccgtgatcc agtaaatgtt gatttatatc
    15541 tgacggcgcc tgaggacagg atacgacgaa ttcatggaag aaaggccgtt gattcaggat
    15601 acgatatccg tgaatcttac tgatctcctt tgcggcatag tgcgctttgt cgtagcataa
    15661 ttttgccact gcctgtaatc cgttccgccc gagcaatgaa aggtaaatgg ctgcagtcaa
    15721 tgcatttaat ccctgattgg aacaaatatt ggaggtggct ttctcccgcc gaatgtgttg
    15781 ttctcgggct gttaatgtca gcacataggc ccgttgccct cgattgtcta ccgtctcacc
    15841 gacgattctt ccggacactt tgcggattaa ttcttttcgt gtggtgaata ttccgaggta
    15901 agggccgccg taagaaagcg gaattcccag cggctgacct tcaccgacga cgatatcggc
    15961 gcccatctca ccgggagttt tcagcagtcc caacgcaatg gggttgatac agacacagag
    16021 taaggatcct ttttcatgaa cggcttttgc aagctcgctg aaatcctgaa tttgtccgaa
    16081 gaaatccgga tattgaacca agaccagcgc ggtatccgta tctattttct cttccaaccg
    16141 ttgcggatta atttcatcaa gggatgcggc tttcggacat tcgatctgca ttccgtcgaa
    16201 atcctgcaga taggttttca gggttagcag gtaatgcgga tgcagtcccg cagataccaa
    16261 aattttcttc cttttcccgt ggtattggtg ataagccaaa ataccggctt cggcgcaggc
    16321 tgtcgcaccg tcataatgag aggcgttaga ggcttccatg ccggttaatc gagccatcag
    16381 gctttgatat tcgtaaacag cttgcaatgt accctgcgag atttccggtt gataaggggt
    16441 ataagcggta taaaaatcgc cgcgactgat gagattatcg attgcggcgg gaatataatg
    16501 atgataagca ccggcaccgg tgaaacaggc tgtatgatcc agggatagat tggctgatga
    16561 aatttcatcg agcagaaagg tgacttccgg ttccgtcaat cccggttgta aattcaattt
    16621 cggaaaacga tatttttccg gaacgacatc aaataattcg ttcaggtctt ttttcccgat
    16681 cgctgccagc atactttcac gatcggcgtc cgtatgcgga ataaacatgg ttcaacctcg
    16741 atcttcgcaa tattttcggt atgcgtctgc gtccattaaa tgagaagtat caaaccctgt
    16801 atcgctgctt aatttgatca gccaggcttc tccgaaagga tcccgattca gcagctcggg
    16861 tgaatcgatt accttattat taatctccgt aacggtgcct gcgacgggag tgtagatatc
    16921 cgatgcggct ttgacggatt caatggttcc gatattgtct tccgccgcaa gtgaatcccc
    16981 ggcggaaacg ctgatttcga caaatacaat gtcagaaagc gaatcctgcg cataatccga
    17041 aattccgatt gtggctttat ttccttcgac cgaaacccat tcgtcattct ttgaataaaa
    17101 ataatttgga tgaatgttca ttgatttccc tccgcttatt catttttctt tattataagt
    17161 ggattcgatt aatatttata gcgatttcgc agagctaaac gaattaagga agacatcgtc
    17221 attccttttg ggtataataa tggaaggaga ggattgtcga aaggaggaat atatgtccat
    17281 taaactcttg gataacagtc tggaattgga aattttcctc gataaagaag attcggaatt
    17341 tgaggacaat atttgcctaa gatttacaga ggactgcccg gaagatgaga aagtttttta
    17401 cggcggtgaa acgaatatct atattacgcg cgatcaagcg ttgcaaatcg ttaaagcgtt
    17461 gggcgctgtt ctggaagaag caaatgaatc aaattgattt cgaaaaaaag cccggtatct
    17521 tcatgaaaac taccgggtct tttttatttt cagtgctttt cttttcgatt caaatatgat
    17581 tgaaaaacag atgtatcgat caactgatgg ccctcgtccg ttaccagtaa tccgaagatt
    17641 ctttcaacgg catgagcaag cgtattgttt aactgtcccc gttccggcgg aaaatccgct
    17701 gttcgaattc cggaaccggc aagtttttcc aaacaaccgg gacggcacca aaacatggat
    17761 ccggcgggat attcgaaata ataatcatgt agcgggatat tcaacaggcc ggcaagggtc
    17821 tccaaattct tccgattggt aaaagtatcg atgtcgatcc cgccggtaag aaatcccttc
    17881 ggaacgacca tatcgatttc gatattttgc cgaaacagtt gtaaaatttc gttcacgcgc
    17941 tgttcggaac cgatcaattc agagaaaagg gattgtcttc gattttcacc cgtggaaaga
    18001 tgagtcgatc tctttgaatg gagcttacag agaaattgat agtctaacgg gaagattgcc
    18061 cgaaaaatct ctaaaaacgg agcgatatcc cgtccgcgat tgggagcgat ataaatatgg
    18121 gcgttcggaa aatatttttg aatttccgag cgaaaattct tttgttcttt cggtaaagag
    18181 ataaaaagat cgaagggttc gggaatatcg gatagcagtt ccgctatttc cggccaaagg
    18241 ccgacataaa aaagatgcag gacgacagcg atctcggaat ttttttcaat tttctcaccg
    18301 aataacctta tttgcagctc ttccggtgac actggggaag aacttgtcgg accggggcgg
    18361 aatatatccg aatgattcaa accgacaatc ttggcagtgg cggtcaaaaa attgcgtatt
    18421 ttcgggagaa aatgaacttc ggaatttgag aaaattgctt tgacctggcg acaataacaa
    18481 atgaatttcc aacctttgct tgatgcgatg tcggaaagct gaaaggcagc ttgtgacagc
    18541 atgcgtcggt ttcttactcc ttcatcgaaa aaggcgctta gttgactgcg ttccgagctg
    18601 agggattcat cgaatgtcat ttttttgcct ccgacctatt tgttcttgat cctgccattc
    18661 cgtcgtttgt ttatgtgcga ttcgcgtatg gttcattttt tatcgactca tccggaaaaa
    18721 tctccgaaat cattttccga aaaataatat cctgtgaata cctctccgta acggtctgtt
    18781 tgccggcttt gccgattttt tttctgaggt ctgcgtcccc tatcaaacga ctgagtttgg
    18841 cttcccattc ggctgctgtt tcggcaagaa aaccgttttg accttcccga acaatataga
    18901 acgactcacc gaccggactg gcaaccaccg gtagttctcc ggccatataa atcaacattt
    18961 tcatggcaca ttttccctgt tccgccgggg tgtcaaatag cggcatgatg ccgatatcca
    19021 tttttttgac aatttccgga atatcgtcat ccgaatattc cggaatttca agtatatgca
    19081 atttttcgat tttgctcaga ggaatcggct gattttttga tccggcgatc aacagctcaa
    19141 aatcgatgcc ggaagcaagc atattcttta acgggtcgac tacaatttcc aactgattca
    19201 gggttgaagg tcctccgatc caaccgatcc gcagcggaga atggggttcc tgtgtggctt
    19261 cgacggaata tttttcaacc ggtatactgc tgggcaataa aatcgtcgaa agattgatcg
    19321 ctttcgcaaa gtcataaagt gcatggctcc cggcaaatac tttccacgca tattgaatca
    19381 ttccacgggt ctttgattca tgcttcacat agagagcgtc atcataatcg aaaatgattt
    19441 ttgaattttg tttcgccaag tatcgcatcg cccggggagg taagagtaca gactgaaaga
    19501 aaatcacatc gtaacgggat tgttgtctca agatccgcaa gacctgaacc caataatatg
    19561 gtaatccgag cccgtggatc agcgatcgaa agatcaaatc ggttattttt tgttttccga
    19621 agatcgatcg atccaatcgt tcgagccaac gttgtacggc agcggcatta atctccatcc
    19681 aatcatagga aatacccgcc tccgaaagag acggaaaaaa ccgttttgcc cggacaatgc
    19741 cgctcgaaaa ataaggtgta cagttcccga cgaaaaggat gttcaaaaga tttccccgcg
    19801 gttgatcaat cagatttgaa tttgattata acgtgagatt tttattcaaa tcaatgcatc
    19861 gtctgtcgca tgaatacaga agcaaaccgg gatgtttgaa aatcaatcga gaacaaccaa
    19921 taatttctct aaaagccccg gtttcctgat caatattgaa aatcttatag aaaagagtca
    19981 gaagcaaaac ttcttattgc aacggttgcg gtccatgata aacttaccgt agcaaattat
    20041 caaccatcta tgagggtata tgtctgattc tgttgcgacc gctgtcgcac atccgaatat
    20101 cgcttttatt aaatattggg gcaatcggga tgattccttg cgtttaccgg caaacggatc
    20161 gatttccatg aatctcgggg ccttggaaac gcggaccacc gtcgctttct cttccgcata
    20221 ccgttccgac cgggtttccg tgaacggctc cttatcggaa ggagaatcgt tgattcgggt
    20281 tgtccatttt atggatcgca ttcggagcct ggcgggagaa gaacgttttg cggaaatcga
    20341 gagccgaaat aattttccga ttggagccgg catcgcttcc tcagctgccg cttttgccgc
    20401 tttagcatta gccggaagtg cggccgccgg gctgcatctc aacgaggcgg aactttcccg
    20461 tttggcacgg ttgggttccg gatcagcctg tcgttcgatc ccccccggtt tctgtgaatg
    20521 gctggcgggc gattccgatg aagaatcgat cgccgtttcc attgcaccgc ccgattattg
    20581 ggctttaacc gacattatcg cggttgtttc cgctcggcat aaggaagtgg gttccagtat
    20641 agggcatcaa cgggcgatga ccagcccgta tcaagcggtt cgggtggcaa cggctccgga
    20701 aagattactg gaatgccgga cagccatatt aaaaagggat tttgatcggc tggcattgat
    20761 ctgtgaaaaa gacagcacca tgatgcacgg ggtcatgatg actcaagaac cgccgttatt
    20821 ttattgggaa cccgcctcat taaagattat gaaaaatatt atgcagtggc gcagagaagg
    20881 gattccctgt ttcttcactt tggatgccgg tcccaatgtt catgttttgt gcccggagga
    20941 ggttgaaaag gaattgaagg agcgcttggg aaaactttcg gaagtggaaa atctgctgtc
    21001 atccggtccc ggggatgcgg ctcaccttgc ggattgatcc gaatgaaaac ggacttttga
    21061 atccgatgga tcaaatcatt aatctgatac ttcccattct gattgtggtg cttgccggct
    21121 attgcgcagc ccgtttccat gtccttgaca aagggaccgg aaatgtttta tgccaatatc
    21181 ttttttattt ttcactgccg tttctgactt ttaccaatat ttatcgggcc ggacttgata
    21241 acctgctgaa cggccggttt atcctgaccg ccattctgac gatcttcggg attattctgc
    21301 ttgcttattt cgtttttcgc tttcttttcc gatcggaagg ccaaaattta atcatgatga
    21361 tttttggtag tttttacgtg aacgcaactt atgtgggagt tccggttgcg tcgatgattc
    21421 tttcatcggt tgtaccccct ttgattatcc tgatgatcca agcttccttc ttttttccgc
    21481 tgacagtttt tctgatggac gtaaacagcg gtaagcaaag tcgcatcaat ttcaaagact
    21541 ccctcttatt gttggttcgg aatcccatat tggtagcgac gctgatcgcc attgttctcc
    21601 tcgtgtttca aatccgggtt cctcaatttg tcttgacgac taccgaattg tttgggaaac
    21661 cggctgccgc caccggattg tttgcgctgg ggttcacttg ctacttaccg gcagaccaaa
    21721 aaattacaaa atcggaaatt cggcgggcgt tgacggtgac tcttttcaag acattccttc
    21781 aaccgctgct tgcttggtcg atcggaagat ttgtttttca tttggactct tggtggttga
    21841 cagcgatcgt cgtttgtgcg atgctcccga ccgccgtgaa ccattttatt gtcagccaaa
    21901 aataccgttg cagcgaaaat gaatccaaac tgatcatttt gttcaccacc ttcggtttta
    21961 caatcatggt cagtttattc ctgtacttct cagccggttt ctgattccga tcaaaatgaa
    22021 agaagatgcc ggaattcggt ttataatccc tttcgaatcg atcggggaag gaggcaaaat
    22081 atgacgatta aaatattggc ggatgatgtt atctcaaaga tagcggccgg tgaagtgatt
    22141 gagcgtcctg cctccgtggt gaaggaactg atcgaaaatg ccatcgatgc cggtgcaaac
    22201 gagatttcga tccgggtgga agattccggc aaaaaactga tcgaagtcag cgataatggt
    22261 tgcggaatga atgccgaaga tctgcgcaat gcgatccaga ggcatgccac gagcaagtta
    22321 cgtgatgcgg atgatctttt ccatattcac accttaggat tccgcggtga agcgttggca
    22381 gcagccggat ccgtttccag aatgacgatt cgttcgcgaa tggcttcgga tttaaacggg
    22441 caaaaaatta cggtggacag cggaaacatc ctcggattga agcaggaaag tatgccggca
    22501 gggacgatcg tcagcgtaga aaatcttttc tgtaacgttc cggcaaggtt gaagttctta
    22561 aaaagcgata caactgaaaa aaatcaaatt gatacgttgg tatcacgata cgcaatggca
    22621 tacccatcga tccactggca atatactcat aatcagcggc cggtgttggc gacgtccggg
    22681 aacggaaaag cgcgcgaggt gctcgcctcg atttacggca tcgatgttgc ccgacaatta
    22741 ttggatgtcg atcttgaaga ggacaatatc catatccgcg gttttatcag tccggtggca
    22801 ttgacccgtt ccaaccgccg ggagatgatt ttctttgtaa acggccgttg gattcaggat
    22861 atccaattga attctgccgt tatgcgggct tatcaatcgt tgatcatggt cggtcgctat
    22921 ccgattgtta ttttgtcgat tgagatggat ccggaagaga ttgatgtgaa tgttcatcct
    22981 tccaaggctg aaatccgttt tcgttccgcg gacaaggtga tctcggcggt tcatcgttcc
    23041 gttcgattcg ggttggtgtc ccagtcacct gtgccggaat taagcaagcc cattcaatgg
    23101 caaacatggg cggagaatcg gagtttgacg ccaaatgtct cttcggcggt ttctcatccc
    23161 gcagatgaag aggagaatac cgatatcggg tttcctcaag cggatccgaa ttctcgaact
    23221 tttcggcagg agaactttgt ttcaccggcc ggtgccgctt ctcactttcc gatcttgcgt
    23281 tggattggtc agatcggcgg aacgtatatt ctggccgaag gaccggatgg gttgtacttg
    23341 attgatcaac acgccgcgca tgagagagtc ctttttgaag aattgttgaa tcgcccgcgg
    23401 gatgaatcca tctcacagat tttattggat ccggaagtgg ttcaacttcc gccgtggcaa
    23461 gccgaccttt tggaaaagaa tattccgatt ctcgaaaaat tgggttttga aatagagccg
    23521 ttcggacctt cggctttccg tatcattgcg attccgtcga tctttaaaaa agggaacccg
    23581 gcggccgcga tccgatcggt ggttgaggat ttcgaagaag acgaggagcc cttaaaagcg
    23641 gaaatcgaaa atctgatcgc cgcacgagtc tgtaaaagca tggctgtcaa aggcgggcaa
    23701 atcttatccg atgaagaaca gcgggcttta attcgggatt tggaaaactg ccagaacccg
    23761 cggacctgtc cgcatggtcg tccgaccatg attcatctga gcattaacct gttggaacag
    23821 caattcggaa gaaaaggtaa aatatagcta agtacattta tccacgccac tcgaaattgc
    23881 aaattatttt ctgtcaaggg ttcgggagga aggagaagga gaatgatgat gcaaattgtg
    23941 acggatagtg gttgtgacct ttcagatgaa caaaaaagag gtctgccgat tcatgtagtt
    24001 ccgttgggct tggtattgaa cggggtttct tatgataaga atcatcctat tactcccgcc
    24061 gaattctatc aaaaaatggt ggagacggat gtttatccga gtacttcgca agcttccgcg
    24121 ggagattttg cggatgtata tcgtgagcta tccgcaaacg gggaagaaat attatccatt
    24181 catatttcat ccggtctgag cggaacactc aattcggcca aagtgggcgg agcgttggtt
    24241 cccaatgcaa aaatcaccta ttgggactcg aagacccttt cgtcagggtt gggatggcag
    24301 gttcaagcag cggcgttggc gatcaaaaaa ggttggtcat tggataagat tttggaaaga
    24361 ttggcactca ttcgcgatca ggtcgacggg atgtttacgt taaaagaaat gcgttacctg
    24421 attcacggcg gtcgcgtcag tcatctggaa ggattaatgg ctcaggttct gaacgtgaaa
    24481 ccgatcatcg cggtggaaca tgagaaaggc acctatgtta ccgccggaaa gctgatcaat
    24541 ttcaaacggg ctttggcgca gattgtcgaa attaattatg agaaattcaa aaacgagaaa
    24601 gtgcgcgctc aaattgttca tgggatcaat ccggaaggtg tggaatatct gaaggaaaaa
    24661 ttacaagaaa agttggacgt tgtttttgaa gaggtcttgc cggtcagtcc ggttttggga
    24721 gcacacaccg ggccgacttt gacagggttg attatcggtc cgatgagttt tttcgccgat
    24781 ctgatctaag acggacggcg gaaagcggga atcaaaagaa aaagcgccgg attgaattcg
    24841 gcgccttttt ataagtaata aggacttaat gaatagcagg atgaaaccgg aaccgttcga
    24901 tcgaacccgt tttttggagt ttctcgaaaa agaatgtggc attctacccg gtgatgcggt
    24961 gttggttgga gtttcgggtg gagctgacag ccttttcctg ctttggcatc tgcatgaaaa
    25021 cggctttcga acgactgccg ccaccttcaa tcatcaattg cggccttccg cagcggatga
    25081 agcggaattt gtgcgccgga cctgtattga gtggtccgtt ccgtttctcg gcgggacaca
    25141 agatgtaatg tcttatgccc gacagcgaaa tttgtcaacg gaagaagcgg ccagagaatg
    25201 ccgctatcga tttctgtttg ctgcggctga aaaaatcgga gccgcggcgg tagcaaccgg
    25261 tcatcatgcc gatgatcagg cagaaaccgt tttgatgcat ttcattcgcg ggagcgggct
    25321 ggatggtttg gccggcatgc gacccaggac ttttttcccg caatggtcga agacgatccc
    25381 ccttgtccgg ccgattttgc cgatcagccg gacggaaatc gagagttggt gtctcgaaaa
    25441 aggcctgcaa ccggtaagtg acgaatccaa tatggataat aattatttcc gcaatcgctt
    25501 acgaaaccgg ctcattccgc tacttgaagc cgaatataac ccccaaatcc gtaaaaatat
    25561 aaacaagacc gcccgggtga ttcagacaga tcttgactgg at
//
LOCUS       DAOSMM010000026        24565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2048_length_24565_cov_15.430396, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000026 DAOSMM010000000
VERSION     DAOSMM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 24565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24565
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2048_length_24565_cov_15.430396"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1670
                     /locus_tag="PK898_05490"
     CDS             147..1670
                     /locus_tag="PK898_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOU19522.1"
                     /translation="MAVKPLSSTPSHPIQIISHDTVYSGCSEKGVCSWKGIPYAKSTA
                     GEGRFRPPQIPEKVDEIVADQFGPACPQHKHLNVPKNENCLNLNIWARFGAKRRPVLF
                     YIHGGSFMSGTGSDPVFDGQELVAAENIVVVTINYRLGVLGILDFSFLGDRFTPNCAI
                     LDVCMALRWTYEHIADFGGNPDNITVMGESAGGSIVSLLPTLPEAAQYISKCVISSGV
                     PTEFWTKSQAQQMAQAYMEFMGISSAEELLAISAERIGDSTQDFTQYTKLGAASYEPS
                     VDGKIIRDFPLVSVQQAEFKKIPMWIGMTRDEMSFLTFSILSRRWGLAKLMEEGTDEE
                     SAALREKIIKIYDETYDKDDARIQLYSDMVIRAPLIWYAEEASQTTHVWFYRMDWSSR
                     IMDKLGIKAFHSSDLPFFFGNFNFPANRIYYQGKEEKKIVKTIGRSIQRDLATFMDSG
                     TLPWETASERFIGKCYGDEIRYEEIMPKEIRDYWRQTTYHERSFISAWQSNNTGTNQ"
     gene            complement(1688..2302)
                     /locus_tag="PK898_05495"
     CDS             complement(1688..2302)
                     /locus_tag="PK898_05495"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOU19523.1"
                     /translation="MEISINPRINKRIQIALNVLKVLSGVLILIMAIYGYRNNLFTSQ
                     ENLTHFIKRFGAWGPFILTVYIAIQVIIPVLPGSLMLVVSPLLFGAFRGFLYSYIGIL
                     IGSVLAFLLAKRYGQVVLSIFVNEKYRQIFDKYTSKKQFTINFALAILLPGFPDDYLT
                     YLAGTTSMSLRTFLIIIVPCKIPGILLYSLGIPSLYKYISKHIF"
     gene            complement(2558..3217)
                     /locus_tag="PK898_05500"
     CDS             complement(2558..3217)
                     /locus_tag="PK898_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOU19524.1"
                     /translation="MNAIVVGGGNVGIYITRLLRENGNQVKIIDYNETITKKLEAEFG
                     KENVFLGNGSNPAVLERTGIEKADVLLAVTGADEANLAVGTLAKYEFGVRKVIGKVNN
                     PLNAWLYTKQFGIDMVINQAELISKIAMEQIDFEDMTILLRLSEEKYSIVQFEIKGNS
                     PSVGKCISDLGLPEESLVISISRNKKLQICKGNTILQAGDHLIALVKDSVNEEFKKHF
                     Q"
     gene            complement(3221..3883)
                     /locus_tag="PK898_05505"
     CDS             complement(3221..3883)
                     /locus_tag="PK898_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOU19525.1"
                     /translation="MEKNNVIIIGCGRMGAGLALRISARGQDVTVIDSNPEAFWRLES
                     PKYTGGKIVGIGFDRDILIKAGIETAYGVVSCTSKDETNAVIGRMARLVFRVPVVIAR
                     LYDPLNVEIYNRLGIRAISTTTWGIDKALNLLSHNPLDTVMEFAHNDVSLIRVEANAM
                     MEGHTVHDFFRVAQVQVVVIERGNSSFIPTFGTKLEAHDVLFVCVDRSQINAFKHSIG
                     ME"
     gene            complement(3885..6704)
                     /locus_tag="PK898_05510"
     CDS             complement(3885..6704)
                     /locus_tag="PK898_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOU19526.1"
                     /translation="MNTLQTNSKNPASSKTATPDIERIYHYTNEEVLAMLESSPAGLS
                     SQQIKERQDRDGKNIIKEAKEKSILLVFLSNFVSVMAILLWVSGFIAILARMPELGIA
                     IWCVNLINGVFSFWQEYQAGKATEALKKMLPSYSRVIRDGTEQQILAEDLVVGDIILV
                     TEGDKISADARLLEATDLQVNQSTLTGESNPVRKVSDPIYRDDVTRSEIPNLIFAGTS
                     VSSGNGKAVVISTGMNTMFGNIAKLTQSMKEEKSPLQKELDKLTQQISAIALGFGVLF
                     FVLAVFFVRDPIAKSFIFSLGMVVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSA
                     VETLGCTSVICSDKTGTLTQNEMTVSNLYLFDRELEVSGTGYAPEGEIRYRGKAVTAD
                     EDEDLRLLLTAAGLCSNARLIEPNENSPQYTVLGDPTEACLGVVARKGGLDPKSLNEE
                     QPRIRELPFDSSRKRMTTIHEVDNRRVAYVKGAPKEVIELCSDRIQEGTKIPLTDSDR
                     EKLMAINDTYARRGLRVLAVAFRDLSESSSLPRAMSQYTPDLIEKNMTFVGLIAMADP
                     ARPEVAQAVAICHKASIRVIMITGDYGLTAESIGKRIGIVKQEHPRIVTGAELSRMSN
                     DDLKAALKDEVIFARVAPEQKYQVVSCLQEMGEIVAVTGDGVNDSPALKKADIGIAMG
                     ITGTDVAKEAADMILLDDNFASIVKAIEEGRTVYQNIRKFLTYILNSNMPEAVPSAFF
                     LFSRGLIPLPLTVMQILTIDLGTDMTPALGLGTEPPEPGIMEKPPRKLSESLLNRNVI
                     VKAFLWYGMLESVFAMAAYFFVNWRNGWPALPLAADGFVYRQATTMTLAAIVFCQIGM
                     VMNCRTDFESIFKVGLFKNKLVIRGIIVEIGLILMIVYVPFLQRIFGTEPLFLSDWLF
                     LSALPFIIIAIEEIRKALIRKKRKA"
     gene            complement(6877..9510)
                     /gene="xdh"
                     /locus_tag="PK898_05515"
     CDS             complement(6877..9510)
                     /gene="xdh"
                     /locus_tag="PK898_05515"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HOU19527.1"
                     /translation="MYKVNINGQDYLSEKDMALMDFLRDVVGITSVKNGCHEGACGAC
                     TVIIDGKTYRSCTQKLSRLEGKKVQTIEGFTQRERDVFSYAFAAVGAVQCGFCIPGMV
                     VCGKNLTDNNPDPTVDDVRVAIRNNICRCTGYAKIVEAILLAAKMLREDLPVPEVKQT
                     GRIGERTMRIDAKIKALGQAKYADDFHFDGMLYGKNVFSKYARAKVLSIDVTPALKLP
                     GVVAVFTAKDIPGHRYIGHLSQDWPGLIDAGEETKFIGDSVAIVVAESLEQAVAGVQA
                     VAVEYEELTPVRNPEEAMAPDAPQIHGEGYEHFGKFIIPKNNLYDHEEVIRGDADAKI
                     ASSKYVAEGTFILPPTEHAFMEPETSVALPDGEDGVLVITGTQGIYDDHREIANYLGI
                     PREKVRIQSAYVGGGFGGKEDMSVQHQAALCAFLLKRPVKVSFSRQESINYHPKRHAM
                     TIHCKIGCDENGILQGIKAHIISDTGAYASLGGPVLQRACTHLGGPYNYHDVSIVGDA
                     YYTNNPPAGAFRGFGVTQSCATVEPLINQLAEMAGISGWEIRYRNAIRPGQSLPNGQV
                     ADQKTAFAETLEAVKPYFDQYEADPDYYVGIASAMKNTGVGVGVPDPGRCNLAIIDGK
                     VHARSSAGAIGQGVQTVMMQIICETTGLLPDRIVIEHPDTKFTPDSSTTTASRQTVFC
                     GEATRRAALQLKQDLDSGNKLEELEGKLYQGEFDYKTDPIGCDKPNPISHITYGYATQ
                     LFIIDREGKVVRVIAAHDSGKIINPMSAEGQVEGGIAMALGYALTEDFPLKDGVPQVK
                     LGTLGIFKANMMPPIQTIFVERIDGDQPAYGAKGIGEITTTMGAPALQNAYYKKDGVF
                     RYRLPLENTFYRKPKTAETTK"
     gene            9953..10822
                     /locus_tag="PK898_05520"
     CDS             9953..10822
                     /locus_tag="PK898_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOU19528.1"
                     /translation="MIDWIIRFVKGAIVGIGAILPGLSGGVLTVIFGIYDPLIRFLAD
                     IRKDFVKNVRYFLPIGIGVAVGILLFSTVVSAAFGKYQAQFVCLFIGFVLGTFPSLFK
                     KAGLKGRNGKHYVIMFVTAILLFLLLLSGQQVLTNVQPNFLVWIGSGAIFAFGFIVPG
                     LSPSNFLIYFGLYDKMADGIKNLDFGVLIPLAIGAVLCILLFAKLMAFLFDRYYSGMY
                     HFILGTVIGSSIAIFPAVVFPAFTAEGLAASGLSFIGSLAFCLILLIIGIIISWQFGK
                     LEERVESQKAAEI"
     gene            complement(10943..11329)
                     /locus_tag="PK898_05525"
     CDS             complement(10943..11329)
                     /locus_tag="PK898_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19529.1"
                     /translation="MDTKKLIYWMPRLLSVLWILILLLTSSQTIPQNSALSENIKWVI
                     RNNQTAIAVALLLLIGWRYEHVAGVCFILLAVFNSVMVTFTDPGSKWYVIGKMLPVTL
                     PGFIISVLFFIAYAQKVYDEMDNEGK"
     gene            complement(11489..12379)
                     /locus_tag="PK898_05530"
     CDS             complement(11489..12379)
                     /locus_tag="PK898_05530"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOU19530.1"
                     /translation="MIKGNANPGNLDQISVRIAENYAGSDLPLYGSSLRLPERKAVIE
                     LLLDFQKLLFPAYFGDPTLIRLPPEHYTHLLLERIRISLRQQIELAMEESPENSKRAQ
                     QICKQLIGRLPAIQELLEKDLTATFEGDPAAQTKEEIVFCYPGFFAIFVYRIAHELYL
                     SKVPLIPRMMTEYAHGKTGIDINPGAVIGEYFFIDHGTGIVIGETTIIGDHVRLYQGT
                     TLGALSPASGQANRGVKRHPTVGNNVIIYSGSTILGGDTIIGNNVIIGGNVFITESVP
                     DNTRVTMKSPELTFREVKDL"
     gene            complement(12395..13333)
                     /gene="cysK"
                     /locus_tag="PK898_05535"
     CDS             complement(12395..13333)
                     /gene="cysK"
                     /locus_tag="PK898_05535"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOU19531.1"
                     /translation="MKIYHSVTELIGHTPLLELSRYETVHNLSATILAKIESFNPTGS
                     VKDRPALAIINRAEADGLLKPGSVIIESTSGNMGIGLASIAAARGYRIILTMPDTMSV
                     ERRQLLKAYGAELVLTEGKLGMEGAVKKAEELAAAIPDSFIPNQFNNPENPASHKSST
                     GPEIWEDTGGKIDIFVAGVGTGGTLSGVGNYLKSKNPQMKVIAVEPAASPVLSEGKAG
                     AHQIQGIGAGFVPKTLDTGVYDEIIPVKDNDAFAVGREIARLEGLLVGISAGAALWAA
                     TRIADRPENNGKVIVTIFPDTGDRYLSGPMFSEEAV"
     gene            complement(13634..14587)
                     /locus_tag="PK898_05540"
     CDS             complement(13634..14587)
                     /locus_tag="PK898_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19532.1"
                     /translation="MNVEAIIIDGMAFALPIFIMAIGGIYSEKSGITMLALEGLQGFG
                     AFTGAIAVLILSPVIGENSPKLIYIAIFAAMIGGMIYSMLHAVLSIHFLANQVISGVV
                     VNILATALTTFLTSLLNESLTGEASNKIQLGAAPRFTVPYLSSVPILGAFFKDVYIFV
                     PFIAFFALIAWYVLYKTPYGLRLRACGENPYSVDAAGGNVNGIRFSAVMICGFLSGLA
                     GIFFAYSTSANFSPSIYMGYGYLAIAALIFGNWKILPTLGVCVFFGLAKSGGYQLTLA
                     LGLSSKVSDLFLTIPYILTLLLLIFFSKHNHPPKAVGQPFD"
     gene            complement(14584..15774)
                     /locus_tag="PK898_05545"
     CDS             complement(14584..15774)
                     /locus_tag="PK898_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19533.1"
                     /translation="MTGQKEANHDKNAFRPIDLLADDRWNKITIPLITILLTVLVASL
                     IMILIGRSPVVAMKSFLQGSGLWPKAVYSPSKGMLTDLFSFLGIFSPMLLASLGVVVA
                     MKGGMFNIGISGQMLASGFLATVFIGYSDLPSTIARPLILLIGILVGGLFGALVGYLK
                     YRFNIHEVVSTIMFNYIISYITGFFINGYYADMITRTSRAVRTAARLQISDLTLMGQK
                     ISFPLGLPIALIAVFLIQFFINRTVSGFEIRMIGSNRICAQYAGVKVGRGLVRAMAIS
                     GMLAGLAGVTMYLGYYNTIVPKELSSMGYDSIAVALLGNLSPSGCIFASFLITIFQKG
                     AVYMSSSTGVAKEIASVITGILLLFSATGMYIKYVAQRTRAKQSVLPSSNGNNQNGNG
                     GNRI"
     gene            complement(15771..17303)
                     /locus_tag="PK898_05550"
     CDS             complement(15771..17303)
                     /locus_tag="PK898_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19534.1"
                     /translation="MDKEIIVEMKHITKLFPGVVANDDISISIQKGEIYALLGENGAG
                     KSTLMSILFGLYQPDHGEIYIRGQKVTLESSRTAAELGIGMVHQHFKLIDNYTIAENI
                     ILCREPIHRLLGFLPAVDLKKANKEIRELSQKFHLEVDPEQKIEDINVSVQQRVEILK
                     MLYREADILIFDEPTAILTPQEIEYLLDIMQELRRNGKTIILITHKLEEVKKIADRCA
                     ILRRGKLIKVLDVSRTSTLEMANLMVGKEFSFDINKTPARCGDVVLDVEHLSVRSENK
                     VEVVHDVSFKVHQGEIFAIAGVSGNGQVEIADAIAGLIPVHSGTIRLNGRDITNLDVR
                     ERTEAGISYIPEDRQQVGMVADMTLAENMAIKSYYREPFAKRGILQPEVIKNYAEKLV
                     RDYDVRTGSGVDTPMGSLSGGNQQKVLVAREIGLNSVLMIFVQPTRGLDIGAISHIHH
                     QILDEREKGKAILLISLELSEIMDLADTIGVIFRGELLKIEDASKMTPSEVGRYMMGV
                     KE"
     gene            complement(17442..18164)
                     /gene="deoD"
                     /locus_tag="PK898_05555"
     CDS             complement(17442..18164)
                     /gene="deoD"
                     /locus_tag="PK898_05555"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU19535.1"
                     /translation="MTIVPSECNEALPGEIAKVVLMPGDPLRAKFVAENYFESPHLFN
                     QLRNMYGYTGIYKGKQISVMGSGMGIPSLGLYASDLYEYYGVEYIIRIGSAGGLADDI
                     RLRDIVIAMSASTNSAYASQYDFPGILAPTADFDLLRKAVKNGEAIGAHLHVGPVFSS
                     DVFKNARKDANERYRDMGMLAVEMETAGLYMTAAYYHKKALSILTISDHVFRQEGLSA
                     IERQESFREMMEIALETAYQSI"
     gene            complement(18181..>18762)
                     /locus_tag="PK898_05560"
     CDS             complement(18181..>18762)
                     /locus_tag="PK898_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU19536.1"
                     /translation="FESGVNYANAKFGTKAEIVELPSYAGTDVTGKNVGGNYIGGFAD
                     EVTGKVVGKALIDKGVDIIFVAAGASGNGVFTAIKEANGVYAIGCDVDQFDDGVKGDG
                     SNMILTSTLKVMHTNVTKQLQAIADGTFVGKNEVLGASTDSTGYVSAEGRQQLSADTL
                     AKLAEAYQLVKEGKIVPAANFNGYTPEDFPGLK"
     assembly_gap    18763..18862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18863..19580)
                     /locus_tag="PK898_05565"
     CDS             complement(<18863..19580)
                     /locus_tag="PK898_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU19537.1"
                     /translation="MKKNSKVIFRVVLILSLVAVFTGCASKTPTAVPPTAAPTAAPTE
                     VAPTGVPTEAVAPTKAEVPVTGGAAAETKALKVGIVTSSGVDDGSFGEDCYNGILDFI
                     KTSPESTVTHVKEPDVSKMIQAVADIITDYDAIVLPGFQFAAVGTVAQENPNTKLILV
                     DTPPTDAEGNTVELPNVYSMTFKEEEGGFFAGIAAAMETKTNKVAVVNGLAYPSNVNY
                     QWGFESGVNYANAKFGTKAEI"
     gene            complement(19638..20708)
                     /gene="mtnA"
                     /locus_tag="PK898_05570"
     CDS             complement(19638..20708)
                     /gene="mtnA"
                     /locus_tag="PK898_05570"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOU19538.1"
                     /translation="MDSSVHKVTHIDNVRLSDDGQSVIIIDQTQLPNRTVYLTLSTAE
                     EMYDAILQLKIRGAPAIGICAAYCLYCLARQIQAPDAESFLEELNRKKMYLNSSRPTA
                     VNLSWALNRVYQAAVENRDRPLEDIIEIMKREAIKIHEEDIAMCRAISEFGLSLLRDG
                     DGVLTHCNAGPLATSRYGTALGPLFLGKERGMEFHVFADETRPLLQGARLTSYELYNA
                     GIDVTLICDNMASLVMKNGWVQACLVGCDRVAANGDTANKIGTSGVAVLAKYYGIPFY
                     VLGPTSTIDRNCKKGNEIQIELRDPEEIKSKFYREPVAPAGVKCYNPAFDVTDHSLIS
                     AIVTEKGICRAPYEESIASLFG"
     gene            complement(20734..21501)
                     /locus_tag="PK898_05575"
     CDS             complement(20734..21501)
                     /locus_tag="PK898_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU19539.1"
                     /translation="MNETIRQLFDLTGRVALVTGVSRGLGRAMAIGLAGAGADVIGIS
                     LQEPIETRKQIEALGKNFYYFHADLAEAEKIPELARQAIAVHGKVDILVNNAGIIGLY
                     PAEEYPLENFDQVFAVNVRGLFLLTQEIGKSMLDRGYGRIIATCSIQSLISGINDSAY
                     VASKHAVAGLVKAFAAEWGGRGVNVNGIAPGFMVTDNTANLRKNSDAVAEITARVPLH
                     RWGQPEDLMGPVIFLASEASRYVNGQLLVVDGGYVIA"
     gene            complement(21498..21944)
                     /locus_tag="PK898_05580"
     CDS             complement(21498..21944)
                     /locus_tag="PK898_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU19540.1"
                     /translation="MNLNADNLIRQLQLEPHAEGGWYRRIWLSNTFIPAESLPQEYGG
                     DRNSAGIIYYLLRRDECSVWHRLKSPETWLWLCGGTLEITLGGTREQPIKDQTFLLTP
                     FGNSDGTQFLTIEPHTWQTAKPVGDEFVLVACIMSPAFTPEDWELA"
     gene            complement(22069..24180)
                     /locus_tag="PK898_05585"
     CDS             complement(22069..24180)
                     /locus_tag="PK898_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU19541.1"
                     /translation="MKNIFKKITEFRFSEAYTPWLILAACILAYGLMIPWIGFYWDDL
                     PFTWIYHTFGSAGLAKYFATKRPALGQIYQVTMPIIGTTPWKWQVFGLFWRWISAVIL
                     WAFLRSVWPNKRFLAICTAFLFAVYPGFDQTYIPIAFSHYFLTESLFFLSLWLTVLAT
                     RLYLKNGREPRFFVFLGLSLILSVINLITTEYFFLLELVRPFFIWVILSQQPDHSHTF
                     KRIIRFEIPYLFIFLCVCIWRLFFFEYQTYNYSPVLWESFKSDPVNTVISLFKTVIHD
                     IWLVSAQAWAKAFTVPNIVDLGRNNWLRYWLIVIGSFLFYLFFFYKSNEKNTPDLPAE
                     NSNDDRSALQAMGIGLFAVFIAGWPYWGTDLPIALGFPNSRFTLSFMLGVSLFIAGII
                     NLWHRRLLRIILASLLIAFGIGLQFQISLDYRVDWNNHKRMIWQMTWRIPALTQHTAI
                     LGNYEPPMHYSDNSLSAELNSIYAPENKSDEMSYMYYFPELREKTYLANFEPNRPITH
                     DYLVATFHGNTSQVLAVYYHPRFCFRVLDPELDPINPLLPEDVRKAAGLSSTKWIETL
                     PESQSVQPDPSVYGREPDRGNWCYTFERADLARQQGNQTQAAEILRSAIDSDVSPRTP
                     SEWLLPIEVFAQTGDWQRAIDLTDRALIPTYEEQPSLLPVICRLWQRIDQNTQASEEK
                     SLAVDRIKRETGCPFESSIPQ"
BASE COUNT         6526 a         5802 c         5446 g         6691 t
ORIGIN      
        1 atgtcgagtc aagttgacaa tcacgattta taaggcttcc gcaaggattt gatatttggt
       61 aaaattattc taaccgtttg gttaaatcgt ttccaaaaac ggatcgagtc aaaaccggat
      121 atatattcag aaatcagcgc tatcttatgg ctgtcaaacc actcagttca accccatctc
      181 accccattca aattatcagt cacgatacgg tttatagcgg ctgttcggag aaaggagtat
      241 gctcttggaa aggaattccg tatgcgaaat caacggcggg agaaggccgt ttccgtccgc
      301 ctcagattcc cgaaaaagtc gatgaaatcg ttgcggatca gttcggtccg gcttgcccgc
      361 agcacaaaca tttaaatgtt cctaaaaatg aaaattgtct gaacttgaat atctgggcgc
      421 ggttcggtgc aaagaggcgg ccggttttgt tttatattca cgggggttca tttatgagcg
      481 gaaccggttc tgatccggtc tttgacggac aagaattggt tgcggcggaa aatattgtcg
      541 tggtgacgat taattatcgt ctgggtgtgt tgggcatcct cgatttttca tttttgggtg
      601 atcgttttac tcccaattgc gcaatccttg atgtttgtat ggcgctccgt tggacttatg
      661 aacatatcgc cgatttcggc ggaaatccgg ataacataac ggttatgggc gaatctgccg
      721 gcgggagcat cgtaagtctg cttcctacgt tacccgaagc ggcacaatat atttccaagt
      781 gcgttatttc aagcggagtg ccgacagagt tttggacgaa aagccaagct caacaaatgg
      841 cacaagctta tatggaattt atggggatct cttccgcaga agaactgctg gcaatctctg
      901 ccgaacggat cggagattcc acacaggatt ttacacaata taccaaattg ggtgcggcat
      961 cgtatgaacc gagcgttgac gggaaaatta tacgggattt tccgcttgta tctgttcagc
     1021 aagccgagtt taaaaaaata cccatgtgga ttggtatgac tcgggacgaa atgtcttttt
     1081 tgacattttc cattctttcc cgccgctggg ggctggcaaa actcatggaa gaggggacag
     1141 atgaggagtc ggctgcgctt cgagaaaaga ttataaagat ttatgacgaa acttatgata
     1201 aggatgacgc aagaattcag ttatattcgg acatggtgat tcgcgcaccg ttaatttggt
     1261 atgccgaaga agcctctcaa accactcatg tctggttcta tcgaatggat tggagcagtc
     1321 gaataatgga taaactcggt ataaaggcat ttcactcttc cgatttaccg tttttctttg
     1381 gtaattttaa tttcccggct aaccgaatat attatcaggg gaaagaagaa aaaaagattg
     1441 taaaaacgat cgggcgcagc atccaaaggg atttggctac ttttatggat tcgggaacac
     1501 tgccctggga aactgcgagt gagaggttta tcgggaaatg ttacggtgat gagatccgct
     1561 atgaggagat aatgccgaaa gaaattcgtg attattggag gcaaacaact tatcacgaac
     1621 gatcttttat ttccgcgtgg cagtcaaaca acacgggaac aaatcaatag attccttgaa
     1681 tttagaatta aaagatgtgc tttgaaatat atttatataa ggacgggatc cctaaactat
     1741 aaagaaggat gcccgggatt ttgcacggta cgatgatgat caaaaaagtt cttaatgaca
     1801 tggaagtcgt accggccagg taagtcagat agtcatccgg aaacccggga agaagtatag
     1861 ccagcgcaaa attgattgta aattgttttt tgctggtgta tttatcgaaa atctgccggt
     1921 atttttcatt tacgaaaatg gaaagaacga cttgcccgta tcgctttgcg agcaaaaaag
     1981 cgagaacgga accgattaag attccaatat agctatagag aaaacctcta aaagcaccga
     2041 ataacagagg gctcacaacc agcataaggc tgccgggcaa aacagggatg ataacctgta
     2101 tcgcgatata gacggtcaaa ataaacggtc cccaggcgcc gaaccgcttg ataaaatgag
     2161 tcaaattttc ctgtgaagtg aacagattat ttcggtagcc atagattgcc atgattaaaa
     2221 taagaacacc tgaaagcacc ttgagcacat tcaatgcaat ctggattctt ttattgattc
     2281 tcgggttgat agaaatttcc atcggcctta tccgtttcct tgctttattt ttatcataga
     2341 atttgaaaaa accgtacctt ttcaaaactt aatcttccga aacttatgat tgtcaatgaa
     2401 tctgctcctt gtagaatcga tatacgtttt tccgcggaga cttgttgcag aagaaagatt
     2461 aagatgatat taacgaaaaa aggagaggaa aagagggcat ttttctttaa aattgttctg
     2521 ccctctttat gatgaccgaa tatcttatcg tttactttta ttggaaatgt ttcttaaatt
     2581 cttcatttac cgaatcttta actaaagcaa tcaaatgatc gccggcttga aggatggtat
     2641 tccctttgca gatttgtagt tttttattgc gagaaatgga aatgaccaaa gattcttccg
     2701 gaaggcctaa atctgatatg catttgccga ccgaaggaga atttcccttg atctcgaatt
     2761 gtacgatgga gtatttctct tcgctcagcc ttaaaagaat ggtcatatcc tcaaagtcaa
     2821 tttgttccat ggcaatcttg ctgatcaatt ctgcctgatt gataaccata tcgataccga
     2881 attgcttcgt ataaagccag gcgttcaatg ggttgttgac ttttccgata acttttcgta
     2941 ccccgaattc atattttgcc aaggttccga cagcgagatt ggcttcgtct gcaccggtga
     3001 ccgccaacag tacatccgct ttttcgattc ccgttctttc aaggaccgcc gggttgcttc
     3061 cgttgcctaa gaaaacattt tctttgccga attccgcttc gagctttttt gtaatggtct
     3121 cattgtaatc aatgattttc acctgattcc cgttttctct gagcagacgg gtaatataga
     3181 taccgacatt tccgccaccc acaacaattg cgttcatgtg ctactccatt ccgatactgt
     3241 gtttaaacgc attgatctga ctgcgatcaa cgcaaacgaa aagcacatcg tgagcttcta
     3301 acttcgtgcc aaaagtggga ataaacgagg agttcccgcg ttcaatgaca accacctgaa
     3361 cctgtgcgac tctgaaaaaa tcatggacgg tgtgcccttc catcatggcg ttggcttcca
     3421 cacgaatcaa agaaacatcg ttatgagcga attccataac ggtatccagc ggattatggc
     3481 tgagcagatt cagcgctttg tcaattcccc aagttgtggt agaaattgca cgaattccca
     3541 accggttgta aatttcgaca ttcaacgggt cgtacagacg ggcaattacg accggtactc
     3601 ggaaaaccaa ccgtgccatc ctgccgatga ccgcatttgt ctcatctttg ctggtgcaac
     3661 ttacgacgcc gtaagctgtt tcgatccccg cttttattaa aatatcgcgg tcaaaaccga
     3721 ttccgacaat ttttccgccg gtgtattttg gggattccaa tcgccaaaaa gcttccgggt
     3781 tcgaatcgat taccgtgacg tcttggcccc tggcagaaat tcggagtgcc aatccggcac
     3841 ccattcttcc gcaaccgata atgatgacat tattcttttc cattttaggc tttcctcttt
     3901 ttcctgataa gcgctttacg tatctcttcg atcgcaatga tgatgaatgg caatgcggat
     3961 aaaaacagcc aatcactcaa gaaaagcggt tctgttccga aaattctctg caaaaaagga
     4021 acataaacaa tcattaaaat gagtccgatt tcaacaataa taccgcgaat cactaatttg
     4081 tttttgaata agccgacttt gaaaatcgat tcgaagtcgg tccggcaatt cattaccata
     4141 ccaatctggc aaaatacaat cgctgccagg gtcattgtgg tggcctgtcg ataaacaaac
     4201 ccgtctgcgg caagcggaag tgccggccaa ccgttacgcc aattgacaaa gaaataggct
     4261 gccatcgcaa aaacggattc caacattcca taccataaaa atgcttttac gatcacgttg
     4321 cgattcaaca aactttcact caattttcgg ggcggttttt ccataattcc gggttcggga
     4381 ggttccgttc ccaaccccag tgcgggtgtc atatccgttc ccagatcgat cgttaagatc
     4441 tgcatgacgg tcagcggcaa ggggatcaat ccgcgagaga acaagaaaaa ggcggatggt
     4501 acggcttcgg gcatgttact gtttaggata tacgtcagga atttacggat attttgataa
     4561 accgtacgtc cctcttcgat tgcttttaca atcgaagcaa aattatcgtc cagcaggatc
     4621 atatcagccg cttcttttgc gacatcggta ccggttatac ccattgcaat tccgatatcc
     4681 gcttttttca atgccggcga gtcattgacg ccgtcacccg taacggcgac aatctctccc
     4741 atttcctgca gacaagacac tacttggtat ttttgttccg gtgcgactcg tgcaaaaatc
     4801 acttcatctt tcagcgccgc tttcagatca tcgttactca ttctgcttaa ttcggcgccc
     4861 gttacaatcc tcggatgctc ctgcttaaca ataccgattc ttttaccgat actctcggcg
     4921 gttaatccgt aatctccggt gatcataata acacgaatcg aagctttgtg acagattgcg
     4981 acagcttgcg ccacttccgg cctggcggga tcggccattg cgatcaagcc gacaaaggtc
     5041 atattttttt cgatcaagtc gggagtatat tggctcatcg ctcgcggcag cgaactcgat
     5101 tcacttaaat cccgaaaagc aaccgcaaga actcgtaagc ctcttctcgc ataagtgtca
     5161 ttgattgcca tcaacttttc ccgatccgaa tcggtcagcg ggatcttcgt cccttcctga
     5221 attcgatcgg agcaaagttc gattacttct ttcggtgccc ctttgacata agcaacccgc
     5281 cggttatcga cctcatggat cgtagtcatt ctttttcttg atgaatcaaa tggcagttca
     5341 cgtattcgag gctgttcttc gttcaaactt tttggatcaa ggccgccttt gcgcgccaca
     5401 actcccagac aggcttccgt gggatcaccc aaaacagtat attgcggact gttttcattc
     5461 ggctcaatta agcgggcgtt gctgcacaga ccggccgcgg tcagcaaaag gcgaagatct
     5521 tcgtcttcat ccgcggtaac ggctttgccg cggtaccgaa tctctccctc cggtgcataa
     5581 ccggtaccgc ttacttccag ctcgcggtca aaaaggtata gattactgac agtcatttcg
     5641 ttttgggtca gtgtccccgt cttatcggag caaatcaccg aggtacaacc cagcgtttcg
     5701 acagccgaaa gctttttaac caacgcatgt tcttttgcca ttcgttgaac agccatagcc
     5761 agagaaaggg tgaccgtcgg taacaaccct tccgggataa atgcgaccac cattccgaga
     5821 gaaaaaataa atgatttggc gatcggatct ctgacgaaga aaacagctaa aacgaaaaac
     5881 aatacgccga accccaaggc aatggctgaa atctgttgcg tcagtttgtc caactctttt
     5941 tgcagcggac ttttttcttc cttcatggac tgggttaact tggcgatgtt tccgaacatc
     6001 gtattcatac cggtactgat cacaaccgct tttccgtttc cggaagagac gcttgttccg
     6061 gcgaaaatca ggttcggtat ttccgaacgg gtcacatcgt cccgataaat cggatccgaa
     6121 accttgcgca ccggattgga ttctccggtt aaggtggatt gattgacctg cagatcggtg
     6181 gcttccaaca atctggcatc ggcggatatt ttatcgcctt cggtgaccaa aataatgtct
     6241 ccgacgacca aatcttcggc caatatttgt tgctcggtac catcccggat gacacgagag
     6301 taagacggca acattttttt gagtgcttcg gttgcttttc cggcttgata ttcctgccaa
     6361 aaactgaaca cgccgttgat cagattgacg caccaaatgg cgatgcccag ctcaggcatc
     6421 cgggccaata tagcgataaa tccgctgacc cataaaagta ttgccatgac gctgacgaag
     6481 ttcgaaagaa agaccaaaag aattgatttt tctttggctt ctttaatgat attttttccg
     6541 tcccgatctt gtcgttcttt aatctgttgc gaactcagcc cggctggtga actttccagc
     6601 atcgccagca cttcttcatt cgtatagtga taaattcttt caatatcggg agtagcggtt
     6661 ttagaactcg ccgggttctt agaattcgtt tgtaaggtgt tcaaaaattt attcctttaa
     6721 ttacgggaac aacaattccg acagcccttt ccgtaatcgg ctagattata gcactattaa
     6781 atattcctta aatttaaggt gcaaaaaaaa tgagataacc tttacaaatt tctaataaaa
     6841 aacaaagccg gtatgatttt gccggctttg tttccattat ttggttgttt cagccgtctt
     6901 cggcttccga tagaaagtat tttccaacgg caaccgatac ctgaagacac cgtctttctt
     6961 atagtaagcg ttctgaagtg ccggagcacc catcgtggtc gtaatctccc cgatcccttt
     7021 tgcaccgtaa gcgggttgat ccccgtcaat tctctctacg aagattgttt gaataggcgg
     7081 catcatgttc gctttgaaaa tgccgagtgt ccctaacttc acctgcggaa cgccgtcttt
     7141 caacggaaaa tcttccgtca aagcgtatcc caacgccatg gcgattccgc cttccacctg
     7201 cccttccgcc gacatcggat taataatttt cccgctgtcg tgagccgcaa tcacccggac
     7261 gactttccct tcccgatcga taataaataa ttgagtcgca tatccgtaag tgatatggct
     7321 gatcggattc ggtttatcac agccgatcgg gtccgttttg taatcaaatt ctccctgata
     7381 cagttttcct tccaattctt ccaacttatt gccggagtcc agatcctgtt tgagttgaag
     7441 agccgcccgc cgggtggctt ccccgcaaaa aaccgtttga cgggaggccg tagtcgtact
     7501 ggaatcgggt gtgaatttgg tatcgggatg ttcgatgacg attcgatcgg gcaacaaacc
     7561 ggtcgtttca caaatgattt gcatcattac agtttgaaca ccctgtccga ttgctcctgc
     7621 agaagaacga gcgtgcactt tcccgtcaat aatcgccaga ttacagcgtc ccggatccgg
     7681 aacgccgaca ccgacgccgg tattcttcat tgcggaagca attccgacat aataatccgg
     7741 atctgcttca tattgatcga aatagggttt aaccgcttcc aacgtctccg caaaagctgt
     7801 tttctgatcc gcaacctgac cgttcggaag agattgtccg ggacgaatcg catttcgata
     7861 gcggatttcc catccggaaa taccggccat ctccgcaagt tgattgatca aaggctcgac
     7921 cgttgcgcaa gattgcgtca ctccgaagcc tcgaaaagct cccgcaggcg ggttattcgt
     7981 atagtatgca tcgccgacga tgctcacgtc atgatagttg tagggaccgc cgagatgtgt
     8041 acaggctcgt tgcaataccg gtccgccaag agacgcatag gcgccggtgt cggaaatgat
     8101 atgtgctttg atcccctgga ggatgccgtt ttcatcacaa ccgattttgc aatgaatggt
     8161 catagcatgc cgtttgggat ggtaattaat actttcctgg cgagaaaaag agacctttac
     8221 cggtcgtttc aacagaaaag cacacaatgc agcttggtgt tgaacgctca tatcttcttt
     8281 cccgccgaat cctcctccga cataagcgct ttggattcga actttttccc gaggaattcc
     8341 caaataattc gcgatttctc gatggtcgtc gtaaattcct tgagttcccg taataacaag
     8401 aacgccgtct tccccatccg gcagtgccac agaggtttcc ggctccataa acgcatgttc
     8461 ggtcggcgga aggatgaaag tgccttccgc aacatatttg gaggatgcga tttttgcgtc
     8521 ggcatcaccg cggatcacct cttcatgatc atacagatta tttttcggga tgataaattt
     8581 tccgaaatgt tcatatcctt cgccgtgaat ttgcggagca tcgggtgcca ttgcttcttc
     8641 aggattacgg acgggtgtca attcttcata ttctaccgca accgcctgaa caccggccac
     8701 agcctgttcc aaactctccg cgaccacgat cgccaccgaa tcgccgatga attttgtttc
     8761 ttctccggcg tcgatcaaac cgggccagtc ctgagaaaga tgaccgatat aacggtgccc
     8821 gggaatatct ttggcagtaa aaaccgcaac cacgccggga agtttcaatg cgggtgtaac
     8881 gtcgatcgaa aggacttttg cccgcgcata tttactgaaa acgtttttcc cgtagagcat
     8941 gccatcaaaa tgaaaatcat cggcgtactt tgcctgcccc aaagctttta ttttggcgtc
     9001 gatacgcatg gtccgttcgc caattcgacc ggtttgtttg acttcgggaa caggcagatc
     9061 ttcccgaagc attttggctg ccaacaggat cgcttcgacg attttcgcat atcccgtgca
     9121 gcgacaaatg ttatttcgaa tagccacccg gacatcatcg actgtcgggt ccggattgtt
     9181 atctgtcaga ttcttcccgc aaacgaccat tcccgggata caaaaaccgc attgaacagc
     9241 accgactgcc gcaaacgcat aggaaaatac atcccgttcg cgttgggtaa aaccttcgat
     9301 cgtctggact tttttccctt ccaatctgga aagtttttgt gtgcaagaac ggtatgtctt
     9361 cccatcaata atcacggtac aggcaccgca ggcaccttcg tgacagccat tttttacgga
     9421 tgtgattccc actacatccc gtaaaaaatc cattaaagcc atatcttttt cggataaata
     9481 atcctgacca ttaatgttta ctttgtacat ttttttccgc tttcaaataa ctaagtattt
     9541 cgattatttg cgcttcatac agatacatcc gggatgagcg gttcaggaaa cggttgggac
     9601 aattcctact atacaaactc cggacgggaa actttccgcc tttttgcctg tcctccgaag
     9661 cagcaggaga atcaccaaaa aagagaagaa agcttccttt cattaatccc gaattccggg
     9721 attgatttca atattatttg attcgatatt tcctcaaacc ttctttaatt atacctaaac
     9781 acagaactga ttcggtatcc cttttacaag accatgtcaa taatttacaa ggatattttc
     9841 tgatgattcg gaaatgccgg atatcctgaa tcagacgaat caatttccta aatatcattt
     9901 cttttgatac aattcacact cgattgaata agaaatactt ttggaggcga cgatgatcga
     9961 ttggataatt cgctttgtaa aaggcgcaat agtggggatt ggagcgattc ttcccggact
    10021 atcgggagga gtcttgacgg tcatttttgg tatttatgat ccgttgattc gatttctggc
    10081 ggatattcgt aaggattttg taaaaaatgt ccgatatttt ctgccgatcg gcatcggcgt
    10141 cgctgtcgga attttattgt tttccacggt ggtttcggcg gcattcggta aataccaggc
    10201 acaattcgtc tgtcttttta tcggttttgt cttgggaact tttccctctc tgtttaaaaa
    10261 agcgggcttg aagggcagaa acggaaagca ttatgtcatt atgtttgtta ccgctattct
    10321 cctgttctta ttactattga gcgggcagca ggtattgacc aatgtacaac cgaatttcct
    10381 tgtatggatc ggcagcggcg cgatattcgc atttgggttt attgtcccgg gattatcccc
    10441 ttccaatttt ctgatatatt tcggacttta tgacaaaatg gcggacggta ttaagaattt
    10501 ggattttggc gtgctgattc cgctggctat cggcgcggta ctttgtattt tattgtttgc
    10561 caaactgatg gcattccttt ttgatcgata ttattcggga atgtatcact ttattctcgg
    10621 aacggttatc ggatcttcca tagcgatatt tccggcggtg gtatttccgg cgtttaccgc
    10681 tgagggatta gcagccagcg gcctttcgtt tatcggttct ttggcatttt gcctgatctt
    10741 gttgattatc ggcatcatca tctcctggca attcggcaaa ctggaagaaa gagtcgagag
    10801 ccaaaaagcg gctgaaattt agcgaatatt ttgtttgaaa aagaaagaac cttgccggtt
    10861 ggaattggcg gcaaggttct tttctatata aaaattcacc attcgaattt tcggaaaaac
    10921 ggattcgaaa gagctttttt ggtcacttcc cttcgttatc catttcatca tagacttttt
    10981 gcgcataggc tataaaaaac agcacgctga tgatgaaacc gggtaacgtg accggaagca
    11041 tttttccgat cacataccat ttgcttcccg gatcggtaaa cgtaaccatg acactgttaa
    11101 aaacagccaa aaggataaag caaacaccgg cgacatgctc atatcgccag ccgattaata
    11161 gcagcaatgc cacagcaatg gcagtttgat tatttctgat gacccattta atattctcag
    11221 aaagcgcact attttgaggg attgtttgag atgatgtcaa taataaaata agaatccata
    11281 agacggataa taaccgcggc atccaataaa tcagtttctt tgtatccatt ttttcacccg
    11341 gttcagaatt tcggaataac ctttctgatt ataagagaca aggaagcggg atgtcaattc
    11401 aaaacgaccg attattttga attgaaaaag gggagttttc tccccctttt ttcaattccg
    11461 tcctcaaaaa tatccgaaat tttcgggctt acagatcttt cacttcacga aaagtcagtt
    11521 ccggtgattt catggtcact cgggtgttat cgggtacaga ttcggttata aaaacattcc
    11581 ccccgattat gacgttattt ccgatgatcg tatccccgcc caaaatggtt gaaccggaat
    11641 agataatgac attattcccg acggtagggt gacgtttgac acctcgattg gcttgcccgc
    11701 tggcgggtga cagcgctccc agagtcgtcc cctgatagag acggacatga tcaccgatta
    11761 tggtggtttc accgataacg atccccgtac cgtgatcgat gaaaaaatat tctccgatga
    11821 ctgcgcccgg gttgatatca atccccgttt tcccgtgggc gtattcggtc atcatccgcg
    11881 ggatgagcgg aactttgctc aaatataatt catgtgcgat ccgataaaca aaaatcgcaa
    11941 aaaagcccgg gtagcagaag acaatttctt ccttggtttg ggcggcggga tcgccttcaa
    12001 aagtagcggt caggtctttt tccagcaatt cctgaatcgc aggcaggcgg ccgatcaatt
    12061 gtttacaaat ctgttgtgca cgtttggaat tttccggact ttcctccatg gcaagctcga
    12121 tttgttgcct gaggctgatc cgaatgcgtt ccagtaataa atgggtgtag tgctccggag
    12181 gtaaccggat caaggtgggg tcgccgaaat acgctgggaa cagtaatttc tgaaaatcaa
    12241 gcagcaactc aattacggct tttctttccg gcagccgaag agaggatccg tacaggggca
    12301 ggtccgatcc ggcataattc tccgctattc ggacagaaat ttgatccaga ttgccggggt
    12361 tggcatttcc ttttatcatt ttgacctcac tcgcttatac tgcttcttcc gaaaacatcg
    12421 gcccggaaag gtagcgatcg ccggtatccg gaaatatcgt gacgatcact ttcccgttgt
    12481 tttcgggacg atcggcaatc cgagttgcgg cccaaagcgc cgctccggct gaaattccga
    12541 ccagcagccc ttccaagcgg gcgatttccc tgccgaccgc gaaagcatcg ttatccttga
    12601 ccggaatgat ttcatcataa acacccgtat ccaatgtttt cggaacaaat ccggcaccga
    12661 taccctgaat ttggtgagct cccgccttcc cttccgataa aacgggagaa gcggccggct
    12721 cgacggcgat gactttcatt tgcgggtttt ttgatttaag ataatttccg actcctgata
    12781 aagtgccgcc ggtaccgact cccgcgacaa aaatgtcgat ctttccaccg gtatcttccc
    12841 atatttccgg tccggtggag gatttatggc tcgccggatt ttccgggttg ttgaattgat
    12901 tcgggataaa actgtcggga atggcagccg ccagttcttc cgcttttttg acggctccct
    12961 ccatgccgag ttttccttcc gttaagacca actcggcccc gtatgccttc agcagttgac
    13021 gtcgttcgac actcatggta tccggcatgg ttaagatgat tcggtatcct ctggcggcgg
    13081 cgatggaggc aagtccgatc cccatatttc cggaggtcga ttcaatgata accgaaccgg
    13141 gttttagcaa accgtccgct tcagcccggt tgattatcgc cagggcgggg cgatctttga
    13201 cgctgccggt gggattgaaa ctttcgattt tcgctaaaat cgtggcggat aagttatgaa
    13261 cagtttcata acgggacagc tccaataacg gtgtatgacc gatcagttcc gtaacagagt
    13321 gataaatttt catgtgtttt tctcctttca aataaaaaaa gcagcaggtt tttttattcc
    13381 tgccgcttcc cgaactccgt gattatttgt gttaatgaat atcaactctt ctttaacaga
    13441 aaagcagcag ataagatccg aaacaacaac agatgcagtt gataacgacc tgatttggaa
    13501 ctcttcccgc tgatttcatt ttattttcct tcataattcc gttttgaata tatcatgcct
    13561 gctatttcgt gtcaattctc ctctgaaaaa tcaggatatt tttcattcgg tttcgcagtg
    13621 tttgatgcca ctcttaatca aacggttggc cgaccgcttt cggaggatgg ttatgttttg
    13681 agaagaagat cagcaataac aaggtcagga tataagggat tgtcaagaac agatccgaaa
    13741 ctttgctgga gagacccaat gccaaggtca gttggtaacc tccggatttt gccagaccga
    13801 aaaacacgca aactcccaag gtcggtaata tcttccaatt tccgaagatc aacgccgcga
    13861 tggcaaggta accgtatccc atgtaaatgc tgggcgaaaa attggcggag gttgaataag
    13921 cgaaaaagat acccgccaat ccggacagaa atccgcagat catcaccgcg ctgaaacgga
    13981 ttccattgac gttcccgccg gcggcatcga cactgtatgg attctcaccg caggcacgta
    14041 accgtaaacc ataaggggtt ttatagagaa cataccaggc gataagcgca aagaacgcga
    14101 tgaagggaac aaagatataa acatctttga aaaatgcccc caatatcggg acagaagaca
    14161 ggtaaggaac ggtaaaacgg ggtgcggctc ccaattgaat cttattcgaa gcttctccgg
    14221 tcaatgattc attcagcaga ctggttaaaa aggttgtcaa agcggtagcc agaatattga
    14281 caaccacgcc gctgatgact tgatttgcca gaaagtgaat gctcaacacg gcgtgaagca
    14341 tggaatagat catgcctccg atcatcgcgg cgaatatcgc gatataaatt aatttcggcg
    14401 agttttctcc gattaccgga gaaaggatca gcaccgctat tgcgccggta aatgcgccaa
    14461 atccttgcaa tccttcgaga gccaacatgg ttatgccgct tttttcactg taaatgccgc
    14521 cgatcgccat gataaaaatc ggtaatgcga atgccattcc gtcgatgatg atcgcttcaa
    14581 cgttcatatg cgatttcctc cgtttccgtt ttgattgttt ccgtttgagg aaggaagaac
    14641 ggattgtttt gcccgggtac gctgcgcgac atatttaata tacattccgg tcgcactgaa
    14701 aagcaaaaga atgccggtaa taacagaggc gatttctttg gcaacgccgg tagatgagct
    14761 catataaacc gcccctttct gaaagatcgt gatcagaaat gaagcaaaga tacaaccgga
    14821 agggctcagg ttgcccaaaa gagcgacggc gatcgaatca tatcccatgg aagaaagctc
    14881 tttcggaacg atggtattgt aataacccag gtacatcgtg actcccgcca aaccggcgag
    14941 catgccggaa atcgccattg ctcgaaccag cccccgaccg actttgacgc cggcatattg
    15001 agcgcaaatc cgattggagc cgatcatgcg aatttcaaac ccgctgacgg tgcggttgat
    15061 aaagaattga atcagaaaaa ccgcgatcag agcgatgggc agccctaagg gaaaactgat
    15121 tttttgtccc attaacgtca aatcgcttat ttgcaacctg gcggcggtcc ggactgctcg
    15181 ggaagttcgg gtgatcatat cggcataata cccattaatg aagaatcccg taatgtaact
    15241 gataatgtaa ttaaacataa tggtggaaac gacttcatga atattaaatc ggtatttcag
    15301 ataaccgacc aatgcaccga acaaaccgcc gaccaaaatc ccgatcagca aaatcagcgg
    15361 tctggcaatc gtgctcggga gatcactata tccgataaat acggttgcca aaaaaccgga
    15421 agccagcatc tggccggaaa tgccgatatt aaacatcccg cctttcattg ccacgacaac
    15481 accgagcgat gccaataaca tcggtgaaaa aattccgagg aacgaaaaga gatctgtcag
    15541 catccccttt gaagggctat aaacggcttt tggccataga ccggatcctt gtagaaagct
    15601 cttcatagcg accaccggac tgcggccgat caaaatcata atcaatgaag cgaccaaaac
    15661 ggtcagcaga attgttatca atgggatggt gattttgttc caacgatcat ccgccaatag
    15721 atcgatcggt cgaaaagcat ttttatcgtg attggcttct ttttggccgg tcattccttc
    15781 acccccatca tatatctgcc cacttcggac ggtgtcatct tggatgcatc ttcaatttta
    15841 agcaattctc ctcggaaaat aaccccgatg gtatccgcca aatccatgat ttcgctcaat
    15901 tccaacgaga ttaacagaat cgcttttccc ttttcccgtt catccagaat ttggtgatgg
    15961 atgtgggaaa ttgcgccgat atccaagccg cgtgtgggct gaacaaaaat catcaatacg
    16021 gaattcaatc cgatttcgcg ggcgaccaat actttttgtt gatttccacc gctgagagag
    16081 cccatcgggg tatccacgcc ggacccggtg cgaacatcat aatctctgac cagtttttcg
    16141 gcataattct tgatgacttc cggctgcaga attcctcttt ttgcaaaagg ctcccgataa
    16201 taggatttga tcgccatatt ttcagccagt gtcatatccg ccaccatccc gacttgttga
    16261 cggtcttcgg gaatatagga tattccggcc tccgttcgct cgcggacatc caggtttgta
    16321 atgtcccggc cattgagacg gatggtaccc gagtgaaccg gaatcaaacc ggcgatggca
    16381 tccgcgattt ccacctgtcc attgccggac acaccggcaa tggcgaatat ttcgccttga
    16441 tgcactttga aagaaacatc atgcacaact tccactttgt tttcgctacg aacggaaaga
    16501 tgttcgacat cgaggaccac gtcgccgcag cgagcgggcg ttttgttgat atcaaatgaa
    16561 aattctttcc ctaccattaa attagccatt tcaagcgttg aagtccggct gacatccaat
    16621 actttgatca gctttccgcg ccgtaaaatg gcacagcgat cggcaatttt tttgacttct
    16681 tctaacttat gggtgatcag gataatcgtt ttcccgttac gccgcaattc ttgcatgata
    16741 tccagaagat attcgatttc ctgtggggtt aggatcgcgg tgggctcatc aaaaatgagg
    16801 atatccgctt ctcgatatag cattttcaag atttccaccc gttgctggac cgaaacgttg
    16861 atgtcttcga ttttttgttc gggatcgact tccaagtgaa atttttgtga aagttcgcga
    16921 atttctttgt ttgccttttt gaggtcgacc gccggcagaa atccgagcag acggtggatc
    16981 ggttcgcggc agagaatgat attctcggcg atggtgtaat tatcgatcaa tttaaaatgt
    17041 tgatggacca tgccgatccc cagttcggca gcggtgcggg aggattccaa tgtgactttt
    17101 tgaccgcgaa tatatatttc tccgtgatcc ggttgatata aaccgaaaag gatactcatc
    17161 agagttgatt ttcccgctcc gttttcaccg agcaacgcat atatttcccc tttttggata
    17221 ctgatggaaa tatcatcgtt tgccacaacc cccgggaaaa gcttcgttat atgtttcatt
    17281 tcaacgatga tttctttatc cataaaaact tactccatcg ttcgtatttc tcatcggtca
    17341 acctcaaaca atgtaaaaaa tcggaatttg tgcggatgga aaaacttgtc attccggatc
    17401 ggattgccct gtttcggaat gacaagtgga tcataattta attaaattga ttgataagcc
    17461 gtttccagag cgatttccat catttcgcga aacgactcct gacgctcaat cgcagacaac
    17521 ccttcctgtc gaaaaacatg gtcggagatc gtcaggatac tgagcgcttt cttgtgataa
    17581 taagccgccg tcatatagag cccggcagtt tccatttcca ccgccaacat ccccatatcc
    17641 cgataacgtt cattggcgtc ttttcgggca tttttgaaca catcggatga aaataccgga
    17701 ccgacgtgaa gatgagcgcc gatggcttca ccgtttttca ccgcttttct caataaatca
    17761 aaatcagcgg taggcgccag aattccgggg aaatcatatt gagacgcata tgcggaattt
    17821 gtcgaagccg acatggcaat aacgatatct cgcaaccgga tatcgtccgc taagccgccg
    17881 gccgaaccga tacgaataat gtattccact ccgtaatatt catataaatc cgaagcgtat
    17941 aaccccagag acgggatgcc catgccgcta cccatgaccg atatttgttt gcctttgtaa
    18001 atccccgtat agccatacat gttgcgaagc tgattaaata agtgcgggga ttcgaaataa
    18061 ttttccgcga caaacttcgc ccggagagga tctccgggca tcagaaccac tttcgcgatt
    18121 tctccgggca acgcttcatt gcattcggaa ggtacgattg tcataaaacg tttaccttgc
    18181 ttatttcaag cccggaaaat cttccggtgt ataaccattg aaattcgcgg cggggacgat
    18241 ttttccttct ttgaccaact ggtaggcttc agccagtttt gctaaggtat cggcggataa
    18301 ttgttgccgc ccttccgcgc tgacataacc ggtggaatcg gttgaggcgc ctaaaacttc
    18361 atttttaccg acaaaagtac catcggcgat tgcctgaagt tgttttgtga cgtttgtatg
    18421 catcaccttg agggttgagg tcagaatcat attgcttccg tcacccttaa caccgtcatc
    18481 gaattgatcg acatcgcatc cgattgcata gacaccattg gcttccttga tagcggtgaa
    18541 tacaccgttt ccggaagcgc ccgctgcgac aaatataata tccacacctt tatctatcag
    18601 cgctttgccg actactttac cggtgacttc atccgcgaaa ccgccgatat aattgccgcc
    18661 gacattcttt ccggtcacat cggttccggc ataggagggc aattccacga tttcagcttt
    18721 tgtaccgaat ttggcattcg catagttaac gcctgactca aannnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnnnnn nncgatttca gcttttgtac cgaatttggc attcgcatag
    18901 ttaacgcctg actcaaaacc ccattgataa ttcacattgg aaggatatgc caagccgttg
    18961 acgactgcga ctttattggt ttttgtttcc atcgccgcag caattccggc gaagaaacca
    19021 ccctcttctt ctttaaacgt catggaatag acatttggta attcgacggt atttccttcg
    19081 gcatcggtcg gcggagtatc gaccaaaatt aacttggtgt tcggattttc ctgggcgacg
    19141 gttccgaccg cggcaaattg gaatcccggc agtacaatcg catcgtaatc ggtgataatg
    19201 tcggctacgg cttgaatcat tttgctgaca tccggttcct tgacatgggt caccgttgat
    19261 tccggactgg ttttgatgaa gtccaaaata ccattatagc aatcttcccc gaacgatccg
    19321 tcgtcgacgc ccgacgaagt tacgattccg acttttaacg cctttgtttc ggcagcggcc
    19381 ccgccggtta cggggacttc tgctttggtc ggtgcgacgg cctctgttgg gactccggtc
    19441 ggagcaactt cggtcggtgc tgcggtgggc gccgcagtcg gaggaacggc ggtcggtgtt
    19501 ttactggcac agccggtaaa aacagcgact aacgacaaaa ttaaaacaac tctgaaaata
    19561 acttttgaat ttttcttcat ttacgatcca tcctttctaa ttaataaaag tttactcatt
    19621 atatcatgat atgaattcta tccgaataag gaggcaatgc tctcctcgta tggagctctg
    19681 catatccctt tttcggttac gattgctgaa attaaggaat gatctgtaac gtcgaacgcg
    19741 ggattatagc atttaacgcc cgccggtgcc accggttccc gataaaattt tgatttaatc
    19801 tcttccggat cccgtaattc aatttggatt tcatttcctt ttttgcagtt tcgatcgatt
    19861 gttgaagtcg gtcccaatac gtaaaaaggg attccgtagt atttggctaa gacagcaacg
    19921 ccactggttc cgattttatt ggctgtatcc ccgttcgccg cgacccgatc acaaccgacc
    19981 aagcaggctt gcacccaccc gtttttcata accaagcttg ccatattgtc acaaatgagt
    20041 gtcacatcga tgccggcgtt gtaaagctcg tagctggtta agcgtgctcc ctgcaaaagt
    20101 ggtcgagtct cgtcagcaaa gacatggaat tccattcctc gttctttccc caaaaacagc
    20161 ggtcccaatg ccgtaccgta acgggaagta gccaacggac cggcgttaca atgggtcaga
    20221 actccgtctc cgtctcggag aagagagagt ccgaattccg aaatggcccg acacatggca
    20281 atatcttctt catggatttt gatcgcttcc cgcttcataa tctcgattat gtcttcgaga
    20341 ggtcggtctc gattctcgac tgcggcttga tagacacggt tcaaagccca acttaaattg
    20401 actgcggtcg ggcgggaact gttcaaatac atcttttttc gattcagttc ctccagaaag
    20461 ctttcggcat ccggtgcctg aatctgccgt gccaggcaat acaaacaata agcggcgcaa
    20521 ataccgatcg ccggcgcccc gcgaatcttg agctgtaaaa ttgcgtcata catttcttct
    20581 gcggttgata atgtcaaata gactgtccgg ttcggcagtt gggtctgatc aataataatg
    20641 acactttgtc catcatcact caatcgtacg ttatcaatat gtgtgacttt gtggaccgat
    20701 gagtccataa aaaactcctt tattctttta tagttaggcg atgacgtatc cgccatcgac
    20761 aacaagcagt tgaccgttca cataacgcga tgcttccgat gccaaaaaga tcaccggtcc
    20821 catcaaatcc tccggctgcc cccaacgatg gaggggaaca cgagcggtga tttccgcgac
    20881 cgcatctgaa tttttacgta aatttgccgt attatccgta accataaaac cgggtgcgat
    20941 tccgtttacg tttacaccgc gtccgcccca ttctgctgcg aaagctttca ccaacccggc
    21001 gaccgcgtgt ttgctcgcca cataagccga gtcgtttata ccggaaatca gggattgaat
    21061 ggaacaggtt gcgatgattc tgccatagcc ccgatcaagc atgctctttc cgatttcttg
    21121 tgtcagaaga aataaccccc tgacgttgac ggcaaagacc tgatcgaaat tctccagcgg
    21181 atattcttcc gcagggtata atccgatgat tccggcgtta ttaaccaaaa tatccacttt
    21241 accgtgaacg gcaatcgctt gccgcgcaag ttccggaatt ttttccgctt ccgctaaatc
    21301 cgcatggaaa taataaaaat ttttaccaag cgcctcgatt tgtttgcggg tttcaatcgg
    21361 ttcttgcagg ctgatgccga ttacatcggc tccggcaccc gccaagccga ttgccattgc
    21421 ccggccgagt ccgcgactga cgcctgttac caacgctacc cgtcccgtca aatcaaacaa
    21481 ttgtcgaatg gtctcgttca tgccagctcc caatcttccg gagtaaaagc cggagacata
    21541 atacatgcca ccaaaacaaa ttcgtccccg accggtttgg ctgtctgcca ggtgtgtggc
    21601 tcaatcgtca aaaattgcgt gccatccgaa ttaccgaaag gcgttaataa aaaagtttga
    21661 tcctttatgg gctgttcccg agttcctccc agtgtgattt ccaatgtacc gccgcatagc
    21721 caaagccagg tttccggtga cttcagacga tgccaaaccg agcattcatc tctgcgtaat
    21781 aagtagtaga ttataccggc gctgtttcga tcgccaccgt attcttgagg aagtgattca
    21841 gccggaatga aggtgttcga aagccagatg cgccggtacc aaccgccttc ggcgtgaggt
    21901 tcaagctgta attgtcggat aaggttgtct gcgtttaaat tcataaaacc tatctccctt
    21961 tatcgttttg gaaaaacaga cttcattatt ttatcagtcg ttcaccgatt ttttacggat
    22021 tgcttacgga aagcaatatt aaggtttatt aaaaaattcg gtacaaagtt attgcggaat
    22081 ggacgattcg aatggacaac ccgtttctct ttttatccgg tcgaccgcca gtgatttttc
    22141 ttcactcgct tgcgtatttt gatcgattcg ctgccataat cgacaaataa ccggcagaag
    22201 ggacggttgc tcttcgtaag tcgggatcaa tgcccgatcg gtcaggtcta ttgcgcgctg
    22261 ccaatcgccg gtttgagcaa aaacttcgat cggcagcagc cattcggagg gtgtgcgggg
    22321 tgaaacatca gaatcgatcg cggatcggag tatttctgcg gcttgagtct gattgccttg
    22381 ctgacgagcc aaatcggcac gttcaaacgt ataacaccag tttcctcgat ctggttcccg
    22441 accataaaca gacggatccg gttgcacgga ttgactttcg ggaagtgttt cgatccactt
    22501 cgtcgaagac aaaccggcgg ctttgcggac atcctccggc agcagcggat taatgggatc
    22561 cagttccgga tccaaaacgc gaaaacaaaa acgcggatga tagtaaacgg caaggacttg
    22621 cgacgtatta ccgtgaaaag tcgcgaccaa ataatcgtgc gtaatcggcc ggttcggttc
    22681 gaaattcgcc agataggttt tttcgcgcaa ttccgggaaa taatacatat aagacatttc
    22741 atcggattta ttttccggtg cgtaaatcga atttaattcc gcgctcaacg agttatcact
    22801 gtaatgcatc ggcggttcat aattgcccag gatggcggtg tgttgagtga gagccggaat
    22861 tcgccaagtc atctgccaaa tcatgcgttt atgattattc caatcgactc gataatctaa
    22921 agagatttgg aattgcaacc cgatcccgaa agcgattaat aaactcgcca aaatgattct
    22981 cagcagacgc cggtgccata agttgataat accggcgata aataaactga caccgagcat
    23041 gaatgacaac gtgaaacggc tgttcggaaa acccaaagca atcggaagat cggtccccca
    23101 ataaggccaa ccggctatga aaaccgcaaa caatccgatt cccatcgctt gaagcgccga
    23161 gcggtcgtcg tttgaatttt ctgccggaag atcgggagtg tttttctcat tcgatttata
    23221 aaaaaagaag agataaaaaa ggaaagatcc gatcacgatc aaccaatagc gcagccagtt
    23281 atttctcccc aaatcgacga tattcggaac ggtaaaggct tttgcccaag cttgagcgct
    23341 taccagccaa atatcatgga taacggtttt gaaaagagat atgacggtat ttaccgggtc
    23401 tgacttgaag ctttcccaaa gaaccgggga ataattatag gtttgatatt cgaagaaaaa
    23461 taatcgccag atacacacgc acaaaaatat gaaaagataa ggaatttcga atcggataat
    23521 ccgcttgaaa gtatgtgagt gatccggttg ttgggataaa atcacccaaa tgaagaaagg
    23581 acgtaccaat tctaaaagga aaaaatattc ggtggtaatg aggttaatca cagataggat
    23641 taaactcaat ccaagaaaaa caaagaaacg gggctctcgt ccatttttca aatataaacg
    23701 cgtggcaaga acagtaagcc aaagagataa aaagaacaaa ctttccgtca gaaaataatg
    23761 agaaaaagcg atcgggatat aagtttgatc aaaacccggg tagaccgcaa aaagaaatgc
    23821 ggtgcaaatc gccaaaaaac gtttattcgg ccagaccgat cgcagaaatg cccagagaat
    23881 cacagcactg atccatctcc aaaaaagacc gaaaacctgc catttccaag gagttgtccc
    23941 gattatcggc attgttacct ggtagatctg acctaaagcc ggtcgtttgg ttgcgaaata
    24001 ctttgcaagt cccgcggagc cgaacgtatg gtagatccag gtaaagggga gatcatccca
    24061 gtagaaaccg atccacggaa tcatcaaacc gtaagccagg atacaggctg cgagaatgag
    24121 ccagggtgta tatgcttcag agaagcggaa ttcggtgatt tttttgaaga tatttttcat
    24181 aaaaacgatt tctgtaattt attgaaagat cgtattcttt atgccaccgc cgaaatactc
    24241 cttcaatttc ttgaaagatc cgtttgaaat tgtcatgcca aagtatatca ttcttcctgc
    24301 gatgaatttt tcaacgaatc aaaacaacct gttcgataag caaaaaaata acggatgaag
    24361 aatctttttg cgattgctat ttcattgaaa atcttttggg gaaagacaga gcaggcgcaa
    24421 catttccata gatattgatt tttcagcgaa gggaaggaaa cctatccgat atatcagctc
    24481 cagaaaaccc aaatcagaat ataagctgaa gtgactgccg ataaagtaaa aggaacactg
    24541 attttcataa aatcttgggt ggacg
//
LOCUS       DAOSMM010000027        24193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2094_length_24193_cov_19.320159, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000027 DAOSMM010000000
VERSION     DAOSMM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 24193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24193
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2094_length_24193_cov_19.320159"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PK898_05590"
     CDS             complement(<1..530)
                     /locus_tag="PK898_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU19542.1"
                     /translation="MFKPVSSKLNVNEMEEGVLKFWKDENIFKKSTDNREGGREYVFY
                     EGPPTANGKPGVHHVISRSIKDVILRYRTMQGCHIIRRGGWDTHGLPVEIEVEKKLGF
                     TNKQQIEEYGIAKFNALCKESAFSNIKEWERMTERVAYWVDFDTAYITYKDEYIESEW
                     QILKNFFERDLLYQGF"
     misc_binding    complement(594..834)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            969..1907
                     /locus_tag="PK898_05595"
     CDS             969..1907
                     /locus_tag="PK898_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19543.1"
                     /translation="MLDILINAISLGILWAILAIGVFFTFRILDIADLSCEGVFPLGA
                     AIAARIISDGGDPFLACLAAIGGGAIAGTFTGILHTKLKIPILLAGILSMTALYSINL
                     RILGKANISLLRMNTVFTKFESMLHTGGKASTFILGLLIVAIIILILWWFLNTELGYS
                     VRATGNNVYMIRAMGVDTDSMFMIGLIIGNALIGLAGAIIAQKQSYADIGMGTGTIVI
                     GLASVIIGEVLFGKKGVLQHMISIVLGSIVYRIIISLILEIDFEIHLGKIVIPISVEP
                     TDLKLFTSIIVIIALSLPVIKQRRASKKASSMLKSI"
     gene            1922..2716
                     /locus_tag="PK898_05600"
     CDS             1922..2716
                     /locus_tag="PK898_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU19544.1"
                     /translation="MLRIENITKIFEPGTVSEKTALVNLDLTLNEGDFVTVIGGNGAG
                     KSSLLNAISGRFFVNEGAIFIDDIDITYQPEYKRAQWIGRVFQDPMMGTAGNMTIEEN
                     LAIALRRGKTRTLNWGMAKGEHDLFYDQLAELELGLEDRIKTKVGLLSGGQRQALTLL
                     MATIKKPKLLLLDEHTAALDPATGKKVLYLTAKLVKENHLTTLMVTHNMKDALEYGNR
                     TIMMSEGKIIADIPAEKKKEMKVEDLMTLFEKSGTESYANDRMLLG"
     gene            2820..3599
                     /locus_tag="PK898_05605"
     CDS             2820..3599
                     /locus_tag="PK898_05605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU19545.1"
                     /translation="MDDQSVDLIYIDPPFNTGHAQKRTSIRVVRDQEGDRVGFQGQRY
                     HSEKTGQHSYDDRFDDFVEFLRPRMEEAYRILKNDGSLYFHIDYREVHYCKIMLDEIF
                     GRDSFLNEIIWAYDYGARTQKKWPPKHDNILFYAKNPNHYTFNVSEIDRIPYMAPGLV
                     GKEKSERGKLPTDTWWHTIVPTNSKEKTGYPTQKPLGIIRRIITASSNPGDLVLDFFA
                     GSGTVGAVCAEMGRDCILIDNNPEAIQIMKQRLGQDAECCI"
     gene            3587..4300
                     /gene="rsmI"
                     /locus_tag="PK898_05610"
     CDS             3587..4300
                     /gene="rsmI"
                     /locus_tag="PK898_05610"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU19546.1"
                     /translation="MLYLVATPIGNLKDITLRAIETLREVDLVVSEDTRKTGLLLQHF
                     EIKKPQISFREDNEKRALPKVMEHLFADKNVALVTDAGSPSVSDPGFTLVREALANHI
                     PVTAIPGATALIPALTLSGLPVHSFTFRGFPPRKSGQRKRFLEADAMSPYTLIYYESP
                     YRLSAFLKDALEVFGDRSAAVANDLTKKFERVDRGRLKELSEKFATEAPRGEYTVVIG
                     GTDFDDKNNIFETSEEQTE"
     gene            4297..5040
                     /locus_tag="PK898_05615"
     CDS             4297..5040
                     /locus_tag="PK898_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOU19547.1"
                     /translation="MNRFFVSPESINGTRVDFPDEIADQIRLVLRLKEEDQVCVLDNS
                     GKAYRVSLRYASGKNPFGEIIGEMPVEAEPLIPVISFIPLTKREKFEWMLQKCTEVGA
                     SCIVPTISERCLIREVKDLDSKMERWQKIVREAAEQSGRGILPSVEMPLDFSQAVKSP
                     APNALKLLFWEEEKDLPIYRVYRSLGEKPNSITIMTGPEGGFGSEEAALAKQNGWHIV
                     TLGKRILRAETAALAALLLTIYESERDEI"
     gene            5189..5908
                     /gene="rnc"
                     /locus_tag="PK898_05620"
     CDS             5189..5908
                     /gene="rnc"
                     /locus_tag="PK898_05620"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU19548.1"
                     /translation="MTKTKTYKEDELPEKFAQRIGLKFNDPMLLCRALTHRSFLNEHP
                     DAIEDNERLEFLGDAILDFIVGAWLYNELPEMPEGELTKYRAALVHTEQLAEFAKQIG
                     LGQALRLGKGELQAGGKTKPALLCDAFEALIGALYLDQGIDAVQNFILPMISESFEDI
                     VSNHKGEDPKSRLQEIIQAENLPIPQYVVVNELGPDHSKIYEIEVMVGDKTLGKGSGS
                     SKQSATKKAAQDALTKLGYGL"
     gene            5966..9568
                     /gene="smc"
                     /locus_tag="PK898_05625"
     CDS             5966..9568
                     /gene="smc"
                     /locus_tag="PK898_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOU19549.1"
                     /translation="MGLKLKSLELQGYKTFARKTRFEFPAPITAIVGPNGSGKSNIAD
                     AIRWVLGEQSYTVLRGRKTMDMIFTGSDQKARSSMASVSILFDNQEEWLPIDYEEVEI
                     TRRAYRSGENEYQVNGQRVRLKDINELLGQSGLSERTYTIIGQGLIDSALSLKPDDRR
                     RFFEEAAGIGLFRSRREEAINRLEITQHNLERINDILGELEPRLKSLQRQSKRAAEYE
                     QLKKDLTVLLKDWYGYHWHKTQQSLVEAMELCESQEAKTKQIQQEYAVIEQDIAAVQE
                     EIRGKRILLGELHDQLSKYHHAMDESSRESSVLDERLNAARRQIAAYREESELIEAEI
                     TLLIERREKVIAESAELKKAGAIAFEQKTQLEAELAAKQKELSALQNKRNESRSLLTK
                     TETTLIQLKARRDETQNRLDALAESYSALQTALVESETILAELQKKQTEIQNDLKAIE
                     SDSLKSEQEQNEIRNKLRDLEEKKAGLSDKLNQNQAQLTRYKAQYEVLNQAEKNLTGL
                     NQGAQFLLKNIGKVIDEKEFRSLGSCFIFPKEVEIAAAAVLGEYLDAILLNRDKWEKA
                     ADLLSSGNNGRAVFISEKDNAKDTAPVSDSKIAAQKSLADLAICDPEYKTLAERLFRK
                     AYLADSFKQASELQPLLQAGESLVTPEGDLFRANGSMATGKEPKYQLISRKREMKELR
                     ELIEDRTEVIQTVRDELNEINQNIQTVQKQAELTAERHRNQSRELEALRKSFNQAVIE
                     TQKREQLIAFQKQRIQENEQQSLTLKAKKESDDAKISELEAELSGLRETVKKLSDAVH
                     QIQLDDLQQQVHHRTLEWTVNEKSIHEAETQIAELTAQIEKNQQRSAQNLEKKTALEQ
                     QILSVQEQIESLKSNNTLSGMNAAEIEAKIAPEENALKSLQQRLETLQSELQIKQAKT
                     SAAEKQLLQQQMEVTRLNDQLDTLKQRIEDDFGLVAFDYKEKVAGQTPLPLGDMVSEL
                     PKVEELPENIKDQISRQKGLINRIGAINPDAIEEYESVNERYNFLLTQSNDLKSADRD
                     LREVIGELDEMMKNAFQETFEKVQAEFKNMFTRLFGGGEARLVLTDPDNINQSGIEIE
                     AKLPGRREQELSLLSGGERSLTSVALVFALLRVSPTPFCVLDEVDAALDESNVGRFCE
                     LLKDLSRETQFIVITHNRNTVETADVIYGITMGKESSSQVVSLRLDEINKDYLI"
     gene            complement(9574..10047)
                     /locus_tag="PK898_05630"
     CDS             complement(9574..10047)
                     /locus_tag="PK898_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19550.1"
                     /translation="MDILNLVDKLEELFSQGRSIPMTHNVIVDEDRMLDIIDQMRISI
                     PDEIKRSQQILAQRDRILAQAKEEHDRTIEIAKEKREQLTSSSEITAEAKKRADQIIS
                     QAYTEAEVIKREADKYALDTLKRLELEMDRTINQVRNGIRTLQRDQESDSNRPES"
     gene            complement(10178..10678)
                     /gene="coaD"
                     /locus_tag="PK898_05635"
     CDS             complement(10178..10678)
                     /gene="coaD"
                     /locus_tag="PK898_05635"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOU19551.1"
                     /translation="MKIRAIFPGSFDPVHYGHIDIAARASRIFDSLVVAVYETPSKNL
                     FFSTEERLEMLRQAMANLPNVEVVSYRGLTIGFAKQINAQVMVRGLRVFSDFDNEFRM
                     ALTNQELDPTIETISLITAHQHTFVSSTTVKEIAELDGDVSSMVPDFVAKRLKEKFAS
                     KKDEII"
     gene            complement(10678..13227)
                     /gene="recG"
                     /locus_tag="PK898_05640"
     CDS             complement(10678..13227)
                     /gene="recG"
                     /locus_tag="PK898_05640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU19552.1"
                     /translation="MLSSTEKIKKFISLEIERGYDNRSVVGGMEKFLPIWRREAPTEG
                     VSEEIIEKVSKFLSEYSDFDKERREQSIRNLLNDLGSPELFDRYKQRYSRAETTNRQN
                     HYSGGENRSKEESSAPDYPEGSKYDHESSNSLRSESSVKPERKPVRIHENHNEASTID
                     SPVKIIPGIGPQNAKALARQGVYTIRDLLYYFPRRYDDYSKFKTINKLLPGEKVTIVC
                     VVYSVENVVRGRYQITEAVVGDGTGMIRVSWFNQPWLIHQIQPGSSYVFSGKIDVFLG
                     RPVMNGPEFEPTEQENLNTNRIVPVYPVNSNLKQNYLRRMIFNTVRHWANLIPEFLPE
                     TVSESADLMPLSKAIEEIHFPDSAELLEAARRRLAFDELFLVQLDVLRHKRNRTSATA
                     NRYRISDERMDCWIKSLPYELTAAQRRVLNEIRGDIDSGKPMNRLIQGDVGSGKTIVA
                     CLAALIISDAGGQSVLMAPTGILAEQHFRSFQDIIEKIRENSGDLNIDPNRIALLTGS
                     TPESEKKEIYEKSASGEIAIVIGTHALLEDPVQFKNLDFIVVDEQHRFGVEQRATLRS
                     KGRNPHLAVMTATPIPRSLALTVYGDLDLSVIDEMPQGRLPVKTFVVNPLGRERVYRH
                     VIKEVKNGHQAFIIYPLVESGDDEQKEGKSAVEASEFLDKEVFPEQRVALLHGRMKGE
                     EKDAILEAFKNKEYDILVSTSVIEVGVDIPNATTIVIEGANRFGLAQLHQFRGRVGRG
                     NEQSYCFLIAENDDAIENERLLAMTQTNDGFKLAELDLNQRGPGDFLGSRQSGFMDFK
                     LASFTDLKLIESARNEALKVFEADPELSSEENKLLKEQLEKLNTIHIGELS"
     gene            complement(13402..15078)
                     /locus_tag="PK898_05645"
     CDS             complement(13402..15078)
                     /locus_tag="PK898_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOU19553.1"
                     /translation="MEFIPNNPQTDQKTNVLTVLNAQQVKSLIRAGLLWLKTNQEIVN
                     SLNVFPVPDGDTGSNMVLTMQAGWNEIVDSPADSVDVLINQVARGALMGARGNSGVIL
                     SQFWRGFSRAIDHQPVLTMELFAGALTEAKRTAYKGVVRPVEGTMLTVLKDTSEKVNQ
                     CLPDCKDFLQLFQITVAAADESVNRTPELLPVLKQAGVVDSGGKGLFFIFEGFLRSLE
                     NKPLDVNPEVIEIVPIDLMKEEESIEPGQDYEVVVDFVPNTQLELSTFYDRLQDIGTS
                     VQVGEGDNNYRMHIHVPAENLYEPINYILTLGTVTKVAIENLVAQMEDLRRQQQCKYD
                     LTPIRDGQIGVVAVSPGDGISRIFASLGVGKIIRGGQTMNPSTEEILNAIESLPTDKV
                     IVLPNNKNVILAANNAITQTNKKVAVIRSKNVPQGISAMLRLDPDGDFDTVVSEMNES
                     LTDIQTGEVTRATRTVEINDVPVNEGQYIVMYNGDLAIAKDGLEEAVFAFLERVNVTD
                     YERITIFYGQDMKEADVNQLVAKIKEKYPDLEVECHYGGQPYYFYILSIE"
     gene            complement(15115..15471)
                     /locus_tag="PK898_05650"
     CDS             complement(15115..15471)
                     /locus_tag="PK898_05650"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOU19554.1"
                     /translation="MSTEQKINGNIYISHRAISTIAYHAALESYGLVGLAPKNKAAGL
                     ANVLIKDPTLGVSIEFDGSGVGIDLYIVVEYGTRITSVASSVADNVSYQVENQTGLTV
                     KYVNVHVRGLRVSCPD"
     gene            15689..16048
                     /locus_tag="PK898_05655"
     CDS             15689..16048
                     /locus_tag="PK898_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU19555.1"
                     /translation="MTEAENFSDCTVLHEDTVKAVQAQMPDEPQFYDLADLYKVFGDT
                     TRIKILWALDIHEMCVCDLAVLLNVTKSAISHQLRILRQENLVKPRREGKEVFYSLAD
                     DHVRSIFEKGLEHINEL"
     gene            16061..18157
                     /locus_tag="PK898_05660"
     CDS             16061..18157
                     /locus_tag="PK898_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU19556.1"
                     /translation="MEKQYKLSGLGCPVCASNIEQKINDIPGVRNATVNFATQTLEIE
                     TESENEIPLFSELEEIVHTYEPETTLKEYSPAENSFEKEERKPKKFRKRIITFVLSAI
                     LAIAGSMQPEASAMRIFLFLLAYLIAGIRVIRKAINSIRHGNWFDENFLMSIASIGAV
                     AIGEFPEAVAVMIFYEVGELLQESAVDHSRKSIASLMNIRPDYANLVTGDGTLRVNPV
                     DVKIGDLIQVKAGERVPLDGIVEEGHSMLDTSALTGESVPREVFPGDPSLSGSINQSG
                     VLTIRVNRTFGESTVNRILDMVENASEKKAATEKFITKFSKIYTPIVIGLAFLIAVIP
                     PLIGGGSWSPWLYRGLVFLVISCPCALVISIPLGFIGGIGKASKKGILVKGSDYLEAL
                     NNVSIAAFDKTGTLTKGTYNVTKVLPASGFSESYLLETAAYAEAYATHPIAKSILRAY
                     GKDIDFNRIEETEEIAGQGIRASVQGKKVLIGNRKMMEGAGITPSQVTENGTVIDVAV
                     DGIFAGSLIISDEVKEDSLSALKRLKTLGVRELVMLTGDKRSVAEEVGKKLGIDRIYS
                     ELLPDQKVEIVEQLLREKPEKTTLMFVGDGMNDAPVLALADIGIAMGKLGSDAAIEAA
                     DVVILTDEPSRLPALIEIARKTHRIVEENIILAISIKVLIMTFGAAGIAGMWMAVFAD
                     VGVMLVAVLNSLRILK"
     gene            complement(18166..18861)
                     /locus_tag="PK898_05665"
     CDS             complement(18166..18861)
                     /locus_tag="PK898_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU19557.1"
                     /translation="MAKLVAVLPAYNEEKHISQVVRELKHYIDDIVVVDDGSVDQTKA
                     LAEAEGVTVLSRGYNCGVGQTTKEGLAWALEHGFDAVILLDSDGQHAPSEIVRFIDKY
                     EQDHPKMIIGYRDYKQMPLFRRFTNTIGKFLLSHAVGQDLPDNQSGYRLVDKELIPLL
                     LESKEAGYNFLVEMIVICLAQGWEIGWVPISTIYGDERSHQNAWYQIKGFTRMCIEAH
                     RKIKAAEKERKND"
     gene            complement(18958..20955)
                     /locus_tag="PK898_05670"
     CDS             complement(18958..20955)
                     /locus_tag="PK898_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU19558.1"
                     /translation="MNNEQSKIYLTIDQKRVEAKDGMTILQIAQANRIDIPTYCVVKD
                     LTPTGACRVCLVSVTFPNGSQRLVTACDTLAIDGMRVRTDTEEVLNSRRQAAEMLLSI
                     SPDTPSLQRLAATYGISQPSYRTQSLQKGCIQCGACVQACREKSAGVLQFRGNGENRV
                     VTTANGSHASACDSCDICIKYCPTGAVTQSLGLGIGDQVKAKKAGRVSARSRSRLFFL
                     SLFILLILMSGFGIQIQGFPNQLFSLMDPLQGSVVLVSGHPEAINYLPALAILILTIL
                     FGRFWCGWICPTGTVLQSFGPNGRRIKSQRLRNLKQIFFVLILLLAAMGSLAFFWTDP
                     IALLTRPFAALFAYSGEKDFASFDWIGIVVLFVPFLIALALNGIEKGFWCRYLCPLGG
                     MLGLFSKFSPFKRKVERESCIECGICAQNCPVGAINPTDGFSSDPAECIQCTECAARC
                     PKYVIRFHGEKIFKSHEFDPDRRELLGIGGAAAAAAAIFAGMSSVNSQPATAETLLPP
                     GAVRDAANDGIKPFLSLCTRCGQCIAACPQKIIKPSLGKGDWTAALTPIVDLSANFCR
                     PDCNRCGQVCPSGAIPQFTVTQKQQAKIGVARVDPSQCIRCYRCVSACPYQAFEQMAD
                     LQTGQIFPHVETEKCNGCGLCLTVCPAREAGAIKIFPLQKG"
     gene            complement(20948..24091)
                     /locus_tag="PK898_05675"
     CDS             complement(20948..24091)
                     /locus_tag="PK898_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU19559.1"
                     /translation="MSEMRYGTFNALEKLKKKLWAEYDHDRATVIVCVWDGPQAADAI
                     AVFTALEETARRKEADVRIELKEIDGRDLEDQGPIVMLMPSYEIISHVVPADAPDLVD
                     HVIQDYRVLGDFFRNDPENESRQLFAKPENQTARRSAKGFRLSKNMSGSEKTPTLGDT
                     ILGAYVLASKKTANLFGRKESTKEKTKSSTRIALEQAFEKITAKYDSSKPNIIICAWE
                     GKCASEAMDLYYAFRQAQDKLGSNARIHIKKIDAGGFKDQGPLVMTLPTRALYVKVRK
                     YDAETIIRRTILRNEVLEGLLYREKVQEEPKTLTLTELAILAADNHSESDENLRNGIQ
                     SQLPAFLPVLEPRKLALIRSDALRMLGLNHLRGLDDGTESSLISASDFYYAEKTKAEA
                     SAKAIGISEAEEEPEEAEFLTVSQLMELAEHPDSQAKKSVKMPATGSRIDRFRYEYLS
                     QLLHSAEIRFAPKLIVLNGNHAAPEEAEAFASALQDVIDEKGLKIKVKIAAATYYACP
                     GVSLLLEPCGILYTDFTSADAERIIQKTLLEGRVLTDLLFSDPLTGNRMLLEDQIPFN
                     RQQARILTEHFYDVAPDSLADYFSAGGYKALSKVLKSSENQSLNEIRESNLRSHYGLG
                     TPLWQKMETCRQQSEDEKYVVAIGHCQPEDADVDGKLLAGSPHQVLEGLIIGAFLVGA
                     EKGYIFIRSANEKAVSLTEQAIRDARIYGLLGKNILGTSFDFDVELIRSDEPAAGGEE
                     TAVLRFIEGRKAEPVNRPPEPEEKGLWNKPTLCDNVKTWAYFPAIFLRGGKTFSEIGS
                     KSTGGSFLLALEGDSLDPCLVEVPAGMKIRNILSFFGNKNILKEVKAVQAGGITGQIL
                     PVDRLDYPFDFSLRTHANTRLGSMLKVYNSHHCVVRTAVNALKVSASEACGRCLTCRV
                     GISRLIDLMEKITSGEGKDVDPELLKEVAAAVRDSSMCPLGAEAPIALLTSLKYFPEE
                     YEAHLNGSCPAGRCPKLTDYLIREDQCTGCGDCQKVCPVHAIEGTEKNPRMIDPIQCI
                     RCGRCPEVCPQGAIHYE"
BASE COUNT         6630 a         5762 c         5473 g         6328 t
ORIGIN      
        1 ttaaacccct gataaagcaa atctctttca aagaaattct tcaggatttg ccattcggat
       61 tcgatatatt catccttgta agtgatataa gccgtatcaa aatcaaccca ataggcgacc
      121 cgctccgtca tgcgttccca ctctttgata ttcgaaaaag ccgattcttt acaaagagcg
      181 ttgaatttcg cgatgccgta ctcttcgatt tgctgtttgt tggtaaatcc caacttcttt
      241 tccacttcaa tttcgaccgg caagccgtgt gtatcccaac cgccgcggcg gataatatga
      301 cacccttgca tcgtacgata gcgaaggatt acatctttaa tggagcgaga aattacatga
      361 tgtacaccgg gttttccgtt tgcggtaggc ggaccttcat agaacacata ctcacggccg
      421 ccttcgcgat tatcggttga ttttttgaaa atattttcat ctttccaaaa cttcagaacc
      481 ccttcttcca tttcattaac attaagtttg gatgaaaccg gtttaaacat aagactcctt
      541 atttgtttcc agccatatcg gctgagagat atctttaata aaaaaaccgt cccgaaaggg
      601 acggggtaaa ttccgcggta ccacccttgt tttcatattc tgcatgaata tgaacactct
      661 ctatccgata acggcggatt atcccgaatc acttactccc gcttcctcct cggaatacag
      721 tttcggatca ttgctcagaa acgattttcc tcgccagctt gatcgtccgg ctttcaccct
      781 cccggattcg ctgtggattc ctactgacgg tactcttttc gtcaacgcat ttcgtatgct
      841 atttaaccat gtttcataca aacggtcaaa cagccgaatt tagagtgtaa ttatatcaat
      901 catcatgaca aattcattat aaaatcgcat gatagaatta cacttgattt aaaaataccg
      961 agggaattgt gctggatata ctgattaatg caatatcatt gggaattcta tgggcgatat
     1021 tggcaatcgg agttttcttt actttcagaa ttctggatat tgccgatttg tcttgcgaag
     1081 gagtattccc gctcggtgcg gcgatcgcag cccggatcat ctcggatggc ggagatcctt
     1141 ttttggcttg tttggcggca atcggcggcg gggcaattgc cgggacattc accggaatcc
     1201 tgcataccaa gcttaaaatt ccgatccttt tagccggcat cctttccatg acagccctct
     1261 attccatcaa tctgagaatt ttgggcaaag cgaatatttc cctcttacgg atgaatacgg
     1321 tcttcacaaa attcgaaagt atgttacata cgggcggtaa agcatccact tttattttgg
     1381 gattattaat tgtcgctata attatattga ttttgtggtg gttcttgaat accgagttgg
     1441 gttattccgt tcgcgcaacg ggcaataatg tgtatatgat tcgggcgatg ggcgtcgata
     1501 ccgacagtat gttcatgatc ggtttgatca tcggtaatgc gttgatcggc ttagccggcg
     1561 caatcattgc tcaaaaacag agttatgcgg atatcggtat gggaaccggc acaattgtga
     1621 tcggcttggc ttcggtaatt atcggggaag tactgttcgg gaaaaaaggc gttcttcagc
     1681 atatgatctc aattgttctc ggcagtattg tttaccgaat tatcatctcc ctgattcttg
     1741 aaatcgactt tgaaattcat ctgggtaaga tagtaattcc gatctccgtt gaaccgacag
     1801 acctgaaact tttcacatcg atcattgtga ttatcgcttt gtctctgccg gttatcaaac
     1861 agcgcagggc ttccaaaaaa gcctcatcga tgcttaagtc gatctgatca ggaaagaaga
     1921 aatgctcaga attgaaaata ttacaaaaat attcgaaccg ggaacggtga gtgaaaaaac
     1981 cgcactggtg aatcttgatt taaccttgaa tgaaggagat ttcgtcacgg tgatcggcgg
     2041 aaacggcgcc ggaaaatcca gcttattaaa tgcgatttcc ggtcgttttt tcgtcaatga
     2101 aggggctatt tttatcgatg atatcgatat tacctatcaa ccggaatata aacgggcaca
     2161 atggatcggc agagtttttc aggatccgat gatggggacc gccggcaaca tgacgatcga
     2221 agaaaatctt gcgattgctt tacgccgcgg gaagactcga accctgaatt ggggcatggc
     2281 aaaaggcgag catgacctct tttatgatca gcttgccgaa ctcgaattgg gactcgaaga
     2341 tcgaattaaa acgaaagtcg gacttctttc cggaggacaa cgtcaggcac tcactctctt
     2401 aatggcgacc atcaagaaac cgaaactgtt gctgttggat gaacatacgg cagcgttgga
     2461 tccggctacc ggtaaaaaag tgctttattt gaccgcgaaa ttggtaaaag aaaatcatct
     2521 gacaacgttg atggtcactc ataatatgaa ggatgcgctt gaatatggga atcggacgat
     2581 tatgatgagc gaagggaaga tcatcgcgga catccctgcg gaaaagaaaa aagagatgaa
     2641 agtggaagac ttgatgacgc tctttgaaaa aagcgggacc gaatcctatg ccaatgatcg
     2701 catgctctta ggttgatcgc aaattttcct tcgatgaccg aatgaatcgg cagtacagct
     2761 gctcatacaa aagaatgata aaaatcttat ttcaggataa tctggaagcc ttgaagcgga
     2821 tggatgatca gtctgtcgat ctgatctata tcgatccgcc cttcaatacc ggtcatgctc
     2881 agaaaaggac ttcgattcgg gtggtccggg accaggaggg ggatcgggtc ggttttcaag
     2941 gacaacgtta ccattcggaa aagaccggtc agcattctta tgacgatcgg tttgatgatt
     3001 ttgtcgaatt tctgcgaccc cgaatggaag aagcttatcg cattctaaaa aatgacggat
     3061 cgctttattt tcatatcgat tatcgcgagg ttcactattg caaaatcatg ctggatgaaa
     3121 ttttcggacg ggattcgttc ctgaatgaaa ttatctgggc atatgattac ggtgcccgca
     3181 cccaaaaaaa atggccgccg aaacacgata atattctgtt ttatgcgaaa aacccgaatc
     3241 attacacatt caatgtcagc gagatcgatc ggattcctta tatggctccc ggtttggtcg
     3301 gaaaagaaaa aagcgaaaga ggaaagcttc ccacggatac ctggtggcac accatcgttc
     3361 cgacaaacag caaagaaaaa accggctatc cgacccagaa accgctggga atcattcgaa
     3421 ggattattac ggcttcttcc aaccccggtg atctggtgtt ggattttttt gccggaagcg
     3481 gtacagtcgg cgcagtttgc gccgaaatgg ggcgggattg cattttgatc gacaacaatc
     3541 ccgaagcgat tcaaatcatg aaacaaagat tgggacagga tgcggaatgc tgtatttagt
     3601 cgccacaccg atcggaaatc tgaaagatat taccttaaga gcgatcgaaa cgctgcgaga
     3661 ggtcgatctg gtcgtcagtg aagacactcg aaagaccggt ttgttgctgc aacacttcga
     3721 aatcaaaaaa ccgcagattt ctttccgcga agataacgaa aaacgggcct taccgaaagt
     3781 aatggagcat cttttcgcgg acaaaaatgt cgcattggtc acggacgccg gatcgccttc
     3841 cgtttccgac ccgggtttta cattggtccg ggaagcactg gcgaatcata ttcccgtcac
     3901 cgcaattccg ggcgcgacgg ctttaatccc cgctcttacg ttatccggtc tgccggttca
     3961 ctcattcacc tttcgcggct ttcctccgcg taagtccgga caacggaaac ggtttttgga
     4021 agcggatgcg atgagccctt acacattgat ttactatgaa agcccgtatc gtctgtcggc
     4081 tttcttgaaa gacgcactgg aagtcttcgg tgaccgcagc gcggctgtcg ccaacgatct
     4141 gactaagaaa tttgaaaggg ttgatcgcgg caggttgaaa gaattatcgg aaaaatttgc
     4201 cacggaagcg ccgcgcggcg agtatacagt cgtcatcggc ggcaccgatt tcgatgataa
     4261 aaacaatatc tttgagacat cagaggagca aacggaatga atcgtttttt tgtctcaccc
     4321 gaatcgatta atggaacgcg agtcgatttt cccgatgaga tcgcggacca aattcgtctg
     4381 gtattgagat taaaagagga agaccaagtc tgcgttttag ataacagcgg gaaagcttac
     4441 cgggtatcgt tgcgttacgc aagcggaaag aaccctttcg gagagatcat cggagaaatg
     4501 ccggtcgaag cggaaccttt gataccggtt atcagtttta ttccgctgac aaaacgggag
     4561 aaattcgaat ggatgctgca aaaatgcacg gaggtcggcg caagttgtat cgttcccacc
     4621 atttccgagc gctgcctgat ccgtgaagtg aaagatctcg attcaaaaat ggaacgctgg
     4681 cagaaaatcg ttcgggaagc cgccgagcaa tcggggcggg gaattcttcc gagtgtcgaa
     4741 atgcctctcg atttctcgca agctgttaag tcaccggctc cgaatgcact caaattgctt
     4801 ttttgggaag aagaaaagga tcttcccatt tatcgtgttt accggtcgct gggtgaaaag
     4861 ccgaattcca tcacgattat gaccggcccg gaaggcggat tcggttcgga ggaagcggct
     4921 ttggcaaaac agaacgggtg gcatatcgtc acgctgggaa aacggatttt gcgggcagaa
     4981 acagccgcat tggcggcgct tttgctgaca atttacgaga gcgaacggga tgaaatctga
     5041 tatactaaaa gaatgaggct ggttaattca ggaagttttt ctacgcttgt tgaagagatt
     5101 cccgaattaa taaattgatc tccgccggag ttcaaaccag ccctaaattt tgtgtgtttc
     5161 cgtttcgaac aaaaaatttg ggggaaaaat gacgaaaaca aaaacatata aagaagatga
     5221 acttccggaa aaattcgccc agcggatcgg tctgaaattt aatgatccta tgcttctctg
     5281 ccgggcactg actcaccgat cttttctgaa tgaacatccg gatgcgatcg aagacaatga
     5341 acggttggaa tttttaggcg acgccatttt agattttatc gtcggagcat ggctgtataa
     5401 cgaacttccc gaaatgccgg aaggcgaatt gacaaaatac cgggcagcgc tggttcatac
     5461 cgaacaatta gccgaattcg cgaaacaaat cggactgggg caggcgttac gtctgggtaa
     5521 aggggaactg caagccggcg ggaaaacaaa accggcactg ctatgcgatg cgtttgaagc
     5581 gcttatcggt gcgctgtatt tggatcaggg aatcgatgca gtccaaaatt ttattcttcc
     5641 gatgatcagc gaatcttttg aagatattgt ctccaaccat aaaggcgaag atccgaaaag
     5701 ccgccttcag gaaatcattc aggctgaaaa tctgcccatt cctcaatatg tagtggtcaa
     5761 tgagctcggc cccgaccatt caaagattta cgaaatcgaa gtaatggtcg gtgataaaac
     5821 actcggcaaa ggcagcggct ccagcaaaca aagcgcaacc aaaaaagccg cacaggacgc
     5881 tttgaccaag ctgggctatg gcttgtagtc cataccgaga aacttctcat tttttatcga
     5941 gcgggaaccc ttttaaataa atacaatggg attaaagctg aaatcactcg aactccaggg
     6001 atataagacc tttgcgcgca aaactcgttt tgaatttccg gctcccatta cggcgatcgt
     6061 cggtccgaac ggatcgggaa aatcaaacat agcggacgca atccgttggg ttttggggga
     6121 gcaatcttat accgtcctgc gcgggcggaa gaccatggat atgatattca ccggctccga
     6181 tcaaaaagct cgctccagca tggcttccgt ttccatcctt tttgataatc aggaagaatg
     6241 gctgccgatc gattacgaag aagttgagat cacccgtcga gcttatcgca gcggggaaaa
     6301 cgaatatcag gtcaacggac agcgtgtccg attgaaagat atcaacgagc tgttaggtca
     6361 atccggttta tcggaaagaa cttacaccat tatcgggcaa gggttaatcg attccgccct
     6421 atctctcaag ccggatgatc gacgtcgctt ctttgaagaa gcagccggaa tcggcttgtt
     6481 cagaagtcgt cgtgaggaag cgatcaatcg ccttgaaatt acccaacaca atttggaacg
     6541 tattaatgac attttgggag aactcgaacc tcgcctgaaa agtttacaac gccaatccaa
     6601 gcgagccgcg gaatatgaac aactgaagaa agatctgact gttctgctga aagattggta
     6661 cggttaccat tggcataaaa cccaacaatc gctggttgaa gccatggaac tctgcgaatc
     6721 acaggaagcc aaaaccaagc aaatccaaca agagtatgcg gtcattgagc aagatattgc
     6781 cgcggttcag gaagaaattc gcggaaagcg tattcttttg ggagagctgc atgatcagct
     6841 ctcaaagtat catcacgcga tggacgaaag cagccgtgaa tcctccgttc tggatgaacg
     6901 gctgaatgca gcccgccgtc agattgccgc ttatcgggaa gaatcggaat tgatcgaagc
     6961 ggaaatcaca ttgctgatcg aacgacgtga aaaagtgatt gctgaatcgg cggaattgaa
     7021 aaaagccggt gcgattgcat ttgaacagaa aacgcaactg gaagcagaac tggccgcaaa
     7081 acaaaaagaa ttatcggctt tgcagaataa acggaatgaa agtcgttctt tgttgacaaa
     7141 aaccgaaaca acgttgattc aattgaaagc acggcgggat gagacacaaa atcgtttgga
     7201 cgctttggcg gaatcctatt ccgctctgca aaccgctttg gttgaaagcg agacgatttt
     7261 ggcggaactg caaaaaaaac aaaccgaaat ccaaaacgat ctgaaggcaa tcgaatcgga
     7321 cagcttgaaa tccgaacagg agcaaaatga aattcgaaac aagctgagag atctcgaaga
     7381 aaaaaaagcc gggctttcgg acaagctcaa tcaaaatcag gcgcaattga cgcgatataa
     7441 agctcagtat gaagttttaa atcaagccga aaagaacctg accggactga accaaggcgc
     7501 tcaatttctg ctgaaaaata tcggcaaagt aatcgatgag aaagaatttc gatcgctggg
     7561 cagttgtttt atcttcccga aagaagtcga gatcgctgcg gctgcggtcc tcggtgagta
     7621 tcttgacgca attcttctga atcgagacaa gtgggaaaaa gccgcggatt tgctttcaag
     7681 cggaaataac ggcagagccg tctttattag cgaaaaagat aacgcaaaag atacggcacc
     7741 cgtttccgat tcgaaaatcg ccgcacagaa atcgctcgcc gacttggcaa tctgtgaccc
     7801 cgagtataaa acgctggctg aacgactttt ccgtaaagct tatttggcgg attcttttaa
     7861 gcaggcgtcg gaattacaac ctttgttaca agccggcgaa tcgttggtga ctccggaagg
     7921 ggacttgttt cgggcgaacg ggagcatggc aaccggtaaa gagccgaaat atcaactgat
     7981 ttccagaaaa agggaaatga aagaactccg agagttgatc gaagaccgga cggaggtcat
     8041 tcagactgtt cgagatgagc ttaacgaaat aaatcaaaac atccaaacgg tgcaaaagca
     8101 agccgaactt accgccgagc ggcatcggaa tcagagccga gaactggaag cgctgcgaaa
     8161 aagttttaat caagccgtca tcgaaacgca aaagcgggaa caattgatcg cattccaaaa
     8221 acaaagaatt caggaaaatg agcagcaaag cctcactctg aaagcaaaaa aagaaagcga
     8281 cgatgcgaaa atcagcgaac ttgaggctga attgagcggt ttacgcgaga cggtgaaaaa
     8341 gctttcggat gccgttcatc aaattcagct ggatgacttg caacaacagg ttcatcatcg
     8401 gacgctggaa tggaccgtaa atgaaaaatc catccatgaa gcggagacgc aaattgccga
     8461 attgaccgct caaatcgaaa aaaatcaaca acgatccgcg caaaaccttg aaaaaaagac
     8521 ggcattggaa caacagatcc tttcggtgca ggaacagatc gaatctttga agagtaacaa
     8581 tacgctttcc ggaatgaacg cagcggaaat cgaagcgaaa atagctcccg aagaaaatgc
     8641 gcttaaaagc ctgcagcaac gtttggagac acttcaatcc gaattgcaaa tcaaacaagc
     8701 caaaacttcg gcggcggaaa aacaattatt gcagcagcaa atggaagtga cccgtttaaa
     8761 tgatcaactc gatactttaa aacaacggat cgaggatgac ttcggtttgg ttgctttcga
     8821 ttataaagaa aaagtagcgg gacaaacacc gcttccgctg ggtgatatgg tctcggagtt
     8881 gccgaaagtc gaagagcttc cggagaatat caaggatcag atctcccgac aaaagggact
     8941 aatcaaccgc atcggagcga ttaaccccga tgcgatcgag gaatatgaat ccgtcaatga
     9001 acgatacaat tttttactga cccaatccaa cgacttaaaa tcggcggatc gggatttgcg
     9061 ggaagtcatc ggggaattgg atgagatgat gaaaaacgca tttcaagaga ctttcgaaaa
     9121 agtgcaggcg gaattcaaaa atatgttcac gcgcttattc ggcggcggag aagcccgcct
     9181 ggttttgaca gaccccgata acatcaacca atccggtatc gaaatcgaag cgaaattacc
     9241 gggcagacga gagcaggaac tttcgttgct ttccggcgga gaaaggagcc tgacgtcggt
     9301 tgcgttggtt tttgctttgc tgagagtcag ccccaccccg ttctgtgtcc tcgatgaagt
     9361 tgacgcggcg ttggatgaat ccaatgtcgg cagattctgc gaactgctca aagatttgag
     9421 tcgtgaaaca caatttatcg ttatcacaca taatcggaat accgtcgaaa cagccgatgt
     9481 catttacggc atcacgatgg gaaaagaatc ttccagccaa gtcgtcagct tgcggttgga
     9541 tgagattaat aaagattatt tgatctgaac ggattaactt tccgggcggt tgctgtcgga
     9601 ttcctgatcc cgttggagcg tacggatccc gttccgaacc tgattaatcg tccgatccat
     9661 ttccaactcc aagcgtttca aagtatccag ggcatatttg tcggcttccc gtttgatcac
     9721 ttcggcttcg gtataagcct gtgaaataat ttgatctgcc cgtttcttgg cttccgccgt
     9781 aatttcgctg ctggaggtca gttgttctct tttttccttg gcaatttcga tggttcgatc
     9841 atgctcttct ttcgcctggg ctaaaattcg atccctttgc gctaagattt gttggcttct
     9901 tttaatttca tccggaatgg aaatccgcat ttgatcgatt atatccagca tgcgatcttc
     9961 atcaacgatg acattgtggg tcattggaat cgagcgtccc tgactgaata attcttccag
    10021 cttgtcaaca agattcagaa tgtccatact tcctccgatt cctcttttga ccgattgatc
    10081 ataataaatt attatgccat gaatgaattc actttacaac attttaaaca tagattcgtt
    10141 taataatctt atcagacgat tctttcgatc ttcgatatta tatgatctcg tcttttttag
    10201 acgcgaactt ttctttcaga cgctttgcca caaaatccgg aaccattgat gaaacatccc
    10261 cgtccagttc ggcgatttct ttcacggttg tcgaggagac aaaagtatgt tgatgagccg
    10321 taataagaga aatcgtctcg atggtcggat ccagctcctg attggtcaac gccatgcgaa
    10381 attcattgtc aaaatccgaa aaaacgcgta atccgcgcac catcacttgc gcattgattt
    10441 gtttggcaaa gccgatcgtc aatcccctgt aactgaccac ttcaacgttg ggcagattcg
    10501 ccatcgcttg ccgcagcatt tccaagcgtt cctctgtaga aaaaaacaga tttttactgg
    10561 gggtttcata aaccgcaacc accagactat caaaaatccg agaagcgcgc gcagcgatat
    10621 cgatatgccc ataatggacc ggatcaaaac ttcccgggaa aattgcccga attttcatta
    10681 actgagttct cctatatgaa tcgtgttgag tttttcgagt tgttctttta ataatttatt
    10741 ttcttccgaa gacagttcag gatcggcctc aaagactttc aaagcttcgt tcctggccga
    10801 ttcgattaat ttcaaatccg taaaagatgc caatttgaaa tccatgaatc ccgattgacg
    10861 acttcccaaa aagtcacccg gaccgcgttg attcaagtcc aattcagcca atttaaaccc
    10921 gtcgttcgtt tgggtcatag cgagcaagcg ttcgttttcg atggcgtcgt cattttcggc
    10981 aatcagaaaa cagtaggatt gctcatttcc ccgacctacc cgaccgcgga attgatgcaa
    11041 ttgtgccaaa ccgaagcgat ttgccccttc gattacaatg gtcgtcgcat tggggatatc
    11101 cactccgact tcgattaccg aagtcgaaac caaaatatcg tattctttat ttttgaatgc
    11161 ctcgagaatg gcgtcttttt cttcgccttt catccggccg tgcaacaacg cgacccgttg
    11221 ttccggaaag acttctttat cgagaaattc gctggcttcc acagcggatt ttccttcctt
    11281 ctgttcgtcg tcaccgcttt cgactaacgg gtaaataata aaagcctgat gtccgttttt
    11341 gacttctttg atcacatgcc gataaacacg ttcccgtccc aacggattga ccacgaacgt
    11401 tttgaccggt aatcttccct ggggcatttc atcgatgacg gataaatcca gatcgccgta
    11461 aaccgtcaat gcgagcgatc ggggaatggg cgtcgcggtc atcaccgcca aatgagggtt
    11521 tctgcctttc gaacgaagtg tcgcacgctg ttcgacgccg aagcgatgtt gttcgtcgac
    11581 aacgataaag tccaagttct tgaattgaac cggatcttcc aataaagcat gcgttccgat
    11641 gacgattgcg atctctccgg aagctgattt ctcgtaaatt tccttctttt ccgattccgg
    11701 agtcgagccg gttaataggg caattcggtt cggatcgata ttcagatcac cggaattttc
    11761 gcgaatcttc tcgatgatgt cctgaaagga acggaaatgt tgttccgcta agattccggt
    11821 cggtgccatc aaaacagact gtccgccggc atcggaaata atcaaagcgg ctaaacaggc
    11881 gacaattgtt ttaccggatc cgacatctcc ctgaatcagc cgattcatcg gtttgcccga
    11941 atcgatatct ccgcgaattt catttaggac tcttcgctga gcagccgtca gttcataagg
    12001 caaactcttt atccagcaat ccattcgctc atcggaaatc cgatagcggt ttgcggtagc
    12061 cgaagtccgg tttctcttat gtcgcaatac atccaactgt acgaggaaga gttcatcaaa
    12121 agccaatcgt ctacgcgccg cctccagtaa ctctgccgaa tccggaaaat ggatttcttc
    12181 aattgctttt gagagcggca tcagatcggc tgattccgat accgtttccg gtaaaaattc
    12241 cggaatcaga tttgcccaat ggcgaacggt attaaatatc atccggcgca gataattctg
    12301 cttcaggttg gaattcaccg gataaaccgg cacaattcgg ttcgtattca ggttttcctg
    12361 ctcggtcggc tcaaattccg gtccgttcat caccggtctt cccaaaaaca catcgatttt
    12421 tcccgagaat acataagagg aacccggttg aatttggtga attagccaag gctggttgaa
    12481 ccagctgacc cgaatcatcc ccgttccgtc cccgacaacg gcttccgtaa tctgatatct
    12541 tccgcgaaca acattttcga cggaataaac cacgcagacg atggtcactt tttccccggg
    12601 aaggagtttg ttgatggttt taaatttcga ataatcatca tatcgacgcg ggaaatagta
    12661 cagtaaatcg cggattgtat agacgccttg ccgggcaagt gcttttgcat tctgcggccc
    12721 gattccgggg ataattttca ccgggctgtc aatcgttgag gcttcattat gattctcatg
    12781 aatccgaacg ggttttctct caggcttgac agatgattcc gatcttagag aatttgaact
    12841 ttcatgatca tatttcgatc cttccggata atcaggcgcg gatgattcct cttttgagcg
    12901 attttccccg ccggaataat gattttgtcg attggttgtt tctgctcttg aataacgttg
    12961 cttataccgg tcaaacagtt ccggtgatcc caaatcgttt aacaaatttc gaatcgattg
    13021 ctcccggcgt tctttatcga aatcactgta ttcggacaaa aatttactga ctttttcaat
    13081 aatttcttcg gatacacctt ctgtcggggc ttcccgacgc caaatcggga gaaatttttc
    13141 cattccccct acaacagagc ggttatcata acctcgctca atttccaaac tgatgaattt
    13201 ctttattttt tctgtggaag acaacatgtc cctattttaa cccatttttt ttcggagccg
    13261 atttctcgat ccggcatttt tttggaattt tcaataaaaa aagggaatta ttccgatgtc
    13321 ttgcatcgat ttatcggatt ttctccgata aatcgacgtt cacatttcga atcccctttt
    13381 cggtttctct gttttgtctt gctattcgat tgaaagaata taaaaataat agggttggcc
    13441 gccgtaatgg cactccacct caaggtccgg atatttctct tttatttttg cgaccagctg
    13501 atttacatcg gcttctttca tatcctgacc gtaaaagatg gtgatgcgtt cgtaatccgt
    13561 tacatttacc cgttccaaaa aggcaaaaac agcttcttcc aaaccgtctt ttgcgatcgc
    13621 caaatcaccg ttatacatga cgatatactg tccctcatta accggtacat cgttgatctc
    13681 taccgtccgt gtcgccctgg tcacttcacc cgtctgaata tcggtcaagg attcattcat
    13741 ctcggagaca acggtatcga aatccccatc cggatccaac cgaagcatcg cgctgatccc
    13801 ctgcggaaca tttttgctgc gaatgacggc gacttttttg ttcgtctgag tgatggcgtt
    13861 attggcagcc aaaatcacat tcttattatt gggaaggaca atcaccttat cggtcggcaa
    13921 ggattcgatc gcgttcaaaa tttcttcagt actggggttc attgtctgtc cgccgcggat
    13981 gatcttcccg acgcctaaac tggcaaaaat acgcgatatt ccgtccccag gtgacaccgc
    14041 taccacaccg atttgaccgt cccggatagg agtcagatca tatttgcatt gctgctgccg
    14101 gcgtaaatct tccatttgag ccaccagatt ttcgatggcg actttggtta cggttcccaa
    14161 tgtcagaata taattgatcg gctcataaag attctcagcc ggaacatgga tgtgcatccg
    14221 ataattatta tcaccctcgc cgacttgcac cgaagttccg atatcttgta atcggtcgta
    14281 gaaagtggaa agttccaact gggtattggg tacaaaatca accaccactt cataatcctg
    14341 cccgggttcg atcgattctt cctctttcat caaatcaatc ggcacaattt caataacttc
    14401 cggatttaca tccaacggtt tattttccaa tgatcttaaa aacccttcaa agataaaaaa
    14461 tagccctttg ccgccggagt ccactacacc ggcttgcttc aaaaccggta atagttccgg
    14521 ggtgcgattt accgattcat ccgctgcggc aacggtgatt tgaaacagtt gcaggaaatc
    14581 tttacaatcc ggaagacatt gattcacttt ttcggatgta tcttttaaaa ccgtcagcat
    14641 cgtgccttca accgggcgaa caacaccttt ataagccgtc cgtttggctt ccgtcagcgc
    14701 gccggcaaat aattccatcg ttaaaaccgg ttgatggtcg atcgcacggg aaaatccgcg
    14761 ccaaaattgg gacagaatca cgccgctgtt tccgcgagcg cccatcaatg ccccccgagc
    14821 tacctgattg atcaacacat cgacactgtc ggcgggtgaa tcgacgattt cattccaacc
    14881 ggcttgcatc gttagtacca tattggatcc ggtatctccg tcgggaacag gaaaaacgtt
    14941 caatgagtta acaatttctt ggtttgtctt cagccaaagt aaaccggctc tgattaaaga
    15001 tttgacttgc tgcgcgttta atacggttaa tacgtttgtt ttttgatccg tttgcggatt
    15061 attgggtata aattccataa ttctcctgat atttctttcc ttagaatccc cccattaatc
    15121 cgggcagctg acgcgtaatc cgcgtacatg gacgttgaca tatttaacgg ttagacccgt
    15181 ttgattttca acctgatagc tcacattatc agccacactc gatgcgaccg atgtgattct
    15241 tgttccgtat tcgacaacaa tatacagatc gatcccgaca ccggatccgt caaactcgat
    15301 actcactccg agagtcggat ctttaatcaa aacattcgca agtccggcag ctttattctt
    15361 cggagctaaa ccaaccaatc cgtaagactc aagagctgca tgatatgcga tggtcgatat
    15421 cgcccgatga gagatatata tgtttccatt aattttctgt tcagtactca ttcaacatct
    15481 tccttttccg cacatatttc cggatcaaat caaaactcaa tgccgatccg gttcgtaaaa
    15541 aaggtttcca gcttttccca ttttaaccgt taatcgaaat gttttgggat gaatatcgaa
    15601 agttatgtca atccccttct tgacaaatga caaaatcgga ataaaataac agttgaacaa
    15661 ttgatcaatt gttttcagaa aggaaattat gacggaagcg gagaatttca gcgattgcac
    15721 ggtattgcat gaagatacgg tgaaagccgt acaggcacag atgccggatg aaccgcaatt
    15781 ttatgatctg gccgatttat acaaagtctt cggagatacg acacgtatca aaattctttg
    15841 ggcgttggac attcatgaaa tgtgtgtctg tgatctggcg gttttattga acgtgacgaa
    15901 atcggctatt tcgcatcaat tacgcatctt acgtcaggaa aatctggtca agccgcgtcg
    15961 tgaagggaaa gaggtttttt attcattggc cgatgaccat gtccgttcga tttttgaaaa
    16021 aggattggaa catatcaacg agttgtaaaa caaggaaagt atggaaaaac aatacaaatt
    16081 atccggttta ggttgtcccg tttgcgcatc caatattgaa caaaaaataa acgatattcc
    16141 cggcgtccga aatgccaccg tgaattttgc cacacaaacg cttgaaatag aaaccgaatc
    16201 ggaaaatgaa attccgctgt tttcggaatt agaggaaatt gtccatactt atgaaccgga
    16261 aacaacgctg aaagaatact ctccggccga aaattctttt gaaaaagaag aaagaaaacc
    16321 gaaaaaattc aggaaaagga taattacgtt cgttttatcg gccattctcg ccattgcggg
    16381 aagcatgcaa cccgaagcga gcgcgatgcg aatttttctg tttcttttgg cctatcttat
    16441 cgccggcatc agagttattc gaaaggcgat caacagtatc cggcatggaa attggttcga
    16501 cgaaaacttt ctgatgagta tcgcttcgat cggagccgtt gccattggag agttcccgga
    16561 agcggttgcc gtgatgattt tttatgaggt cggagagtta ttgcaggaat cggcggtcga
    16621 tcattcgcga aaatcaatcg caagtctgat gaatatccgc cccgattatg ccaatttagt
    16681 cacgggcgac gggacgcttc gggtaaatcc ggtcgatgtc aaaatcggag atttaattca
    16741 ggtgaaagcg ggcgaaaggg tcccgttgga tggcatagtg gaagaaggac attcgatgtt
    16801 ggatacgtcc gctctgacgg gagaatccgt accgcgggaa gtttttccgg gagatccatc
    16861 tttgtccggc tcgattaatc aatcgggtgt actgacgatt cgggtaaaca gaactttcgg
    16921 cgaatcgaca gtcaatcgaa ttttggatat ggtcgaaaat gcgagcgaga aaaaagcggc
    16981 taccgaaaaa tttatcacca aattttcaaa gatttacacc ccgatcgtta tcggactggc
    17041 atttctaatc gcggttattc caccgctcat cggtggcggt tcttggagcc catggcttta
    17101 tcgcggattg gtttttttgg tcatatcatg tccttgcgca ctggtcattt ccattccgtt
    17161 ggggttcatc ggcgggatcg ggaaagcttc caaaaaagga attctggtca aaggatccga
    17221 ttatttggaa gcgttgaata acgtcagtat cgcagctttt gacaagaccg gaacattgac
    17281 aaaaggaacc tacaacgtca ccaaagtgct gcctgcgtcc ggattctccg aaagttatct
    17341 gttggaaacc gcagcgtatg cggaagctta tgccacccat cccatcgcaa aatcaatcct
    17401 gcgtgcttac ggaaaagata tcgatttcaa ccgaattgaa gaaacggaag agattgccgg
    17461 gcagggaatt cgagcctccg ttcaagggaa aaaagtcctg atcggaaatc gaaaaatgat
    17521 ggaaggagcc ggtatcacac catcgcaggt caccgaaaac ggaaccgtca ttgatgtggc
    17581 ggtcgacggg atatttgctg gcagcctgat tatttcggat gaagtgaaag aggacagtct
    17641 ctcagcatta aagagattga aaaccctcgg tgtcagagaa ctggtaatgt tgaccggtga
    17701 caaaagatcc gtcgctgagg aagtcggtaa aaaattaggg atcgatcgga tttacagtga
    17761 actgttgccg gatcaaaaag ttgaaatcgt cgaacagttg ttacgcgaaa aaccggagaa
    17821 gactacgctg atgtttgtcg gcgacgggat gaacgacgca ccggttctgg cactggccga
    17881 tatcgggatc gccatgggaa agctcggatc cgatgcggca atcgaagcgg cggatgtggt
    17941 cattttgacg gatgaacctt ccagattacc ggctttgatc gaaatcgcca gaaaaactca
    18001 tcggattgtc gaagaaaata tcatattagc gatttcgatc aaagtattaa ttatgacttt
    18061 cggagccgcc ggcatcgccg gcatgtggat ggccgttttt gcggacgtgg gagtgatgtt
    18121 ggttgccgtc ctgaattccc tcagaatatt aaaataagga cgccgtcaat cgtttttccg
    18181 ctccttttcc gcagctttga tttttcgatg agcctcgata cacattcggg taaaaccttt
    18241 aatttgatac caggcgtttt gatgacttcg ttcatccccg taaatcgtcg aaatcggtac
    18301 ccaaccgatt tcccatcctt gagccaggca aatgacaatc atttccacca aaaagttata
    18361 tccggcttct ttggattcca acagaagcgg tattagttct ttatccacca atcgatagcc
    18421 tgattgatta tcgggcagat cctgacctac ggcatgagaa agcagaaatt taccaatggt
    18481 attggtaaat cgcctgaata acggcatctg tttataatcc cgatatccga tgatcatttt
    18541 tggatgatcc tgttcatatt tatcgataaa tcgaacaatt tctgacggag catgttgccc
    18601 atctgagtct aacaggataa ccgcatcgaa cccgtgttcc agcgcccaag ccaacccttc
    18661 ttttgtcgtc tgtcccacac cgcagttata tcctcgggat aaaaccgtaa caccttccgc
    18721 ttcggctaaa gctttggtct gatcgaccga tccgtcatcc accacaacga tatcatcaat
    18781 ataatgcttc agttcgcgaa ccacttggga gatatgtttt tcttcgttat aagccggtaa
    18841 gaccgctacc agttttgcca tgccttttcc cttccttcat ctgattgatc tttttcggat
    18901 cccggactct tcacaatctt tgtcataaaa aagatttcga cccggatcgc cgcccgttta
    18961 tcccttttgc aacggaaata tttttatcgc accggcttcc cgagccggac agacagtcag
    19021 gcataatccg caaccgttgc atttttcggt ttcgacatga ggaaaaattt gaccggtttg
    19081 aagatccgcc atctgttcga aagcctgata tggacaagcg ctgacacaac ggtaacaacg
    19141 aatacattga gaagggtcca cgcgtgccac cccgattttg gcctgctgtt tttgagtgac
    19201 agtgaactgc gggatcgctc cgctcggaca aacctgaccg caccggttac aatccggccg
    19261 gcagaaattg gcgctgagat ccacgatcgg ggtcagtgcg gcggtccaat cgccttttcc
    19321 taaagaaggt tttatgattt tttggggaca tgccgcaatg cactgcccgc agcgcgtgca
    19381 cagcgacagg aacggtttta tgccgtcgtt tgcggcatcg cgaacagcac ccggaggaag
    19441 gagcgtttcc gccgtcgccg gttgagaatt taccgaactc atccccgcaa aaatcgcggc
    19501 tgcggcagca gccgccccgc cgattcccaa caattcgcgg cggtccggat caaattcatg
    19561 acttttaaag attttttcac catgaaaccg gataacatat ttaggacagc gagccgcgca
    19621 ttccgtgcac tgaatacatt ccgccggatc ggacgaaaaa ccgtccgtgg gattgatcgc
    19681 tccgaccggg cagttttggg cacaaattcc gcattcgata caactttccc gctcgacttt
    19741 tctcttaaaa ggtgagaact tggaaaagag acccaacatc ccacctaacg gacagagata
    19801 acggcaccaa aagccctttt caatcccgtt caatgccaac gcaatcaaga aggggacaaa
    19861 aagaacaacg ataccgatcc aatcaaacga agcaaaatct ttttccccgg agtaagcgaa
    19921 caaagcagca aaaggtcggg tcaaaagagc aatgggatcc gtccaaaaaa aggccaagct
    19981 tcccatcgcc gccagaagca aaatcagtac aaagaaaatc tgtttgagat tgcgtaatcg
    20041 ttggcttttg atccgccgtc cgttcggtcc gaacgactgc aacacggtcc cggtcggaca
    20101 gatccatccg caccaaaatc ttccgaaaag aatcgtcaga atgaggatcg ccagcgccgg
    20161 caggtagttg atcgcctccg gatgacccga gaccagaaca acactccctt gcagaggatc
    20221 catcagagaa aacaattgat tcggaaaacc ttgaatttga attccaaaac ccgacattaa
    20281 aatcaataag ataaaaagcg acagaaaaaa caacctgctt cgagaacgtg cggaaactct
    20341 tccggctttt tttgccttaa cttggtcgcc gattcccaat ccgagacttt gcgtgaccgc
    20401 acccgtcggg caatatttaa tacagatatc acaggaatca catgccgatg catgcgaacc
    20461 gtttgctgtc gtaacgaccc gattttctcc gttcccccga aattgcaaca cgcccgcact
    20521 tttttcccga catgcttgga cgcacgcacc gcattgaata caacccttct gaaggctttg
    20581 agtgcgataa gaaggttggg aaataccgta agtcgcagcc agccgctgaa gcgacggcgt
    20641 atcgggggag atcgaaagta acatttcggc cgcctgacga cgagaattca atacttcttc
    20701 cgtatccgtc ctcacccgca ttccgtcaat cgccaaagta tcgcaagctg tcaccaagcg
    20761 ttgtgatccg ttcggaaacg ttaccgagac cagacagacc cgacaggccc cggtcggtgt
    20821 caaatctttt acgacgcagt atgtgggaat atcgattcga tttgcttggg ctatttgcag
    20881 aatcgtcatc ccatccttcg cttctacgcg tttttgatca atggtaagat agatcttgct
    20941 ctgttcgtta ttcataatga atcgctccct gaggacaaac ttccggacac cgaccgcagc
    21001 ggatacattg aattggatca atcattcggg ggttcttttc cgtgccttcg attgcatgaa
    21061 ccggacaaac cttttgacaa tccccgcatc ccgtgcattg atcttcccga atcaggtaat
    21121 cggtcagttt cggacaacga cccgccggac aacttccgtt caaatgggct tcatattctt
    21181 ccggaaaata ttttaaagaa gtcagcaacg cgatcggcgc ttccgcaccc agcgggcaca
    21241 ttgaactgtc tcggaccgcc gccgcgactt ccttcaacaa ttccggatcg acatcttttc
    21301 cttctccgga agttattttc tccattaaat caatcaagcg ggaaatgccg acccgacagg
    21361 tcagacagcg accgcaggct tccgaagcac ttacttttaa tgcattgaca gctgtacgga
    21421 cgacacagtg atgagagtta tacactttca gcatcgatcc cagccgggta tttgcatgtg
    21481 ttcgcagtga aaaatcaaac ggataatcca aacggtcaac cggaagaatc tgaccggtaa
    21541 ttccgccggc ctgaaccgct ttcacttcct tgagaatgtt cttgttgccg aaaaacgaca
    21601 gaatattccg aattttcatg cctgccggta cctcgaccaa acaaggatcg agagaatctc
    21661 cctctaaagc caacaaaaac gatcctccgg ttgatttaga tccgatttcc gaaaaagtct
    21721 ttcctccccg caaaaagatc gccgggaaat aagcccaggt tttcacgtta tcacataagg
    21781 tcggtttatt ccacaacccc ttttcttccg gttccggcgg ccggttgacc ggctcggctt
    21841 tccgaccttc gataaatctc aggacagccg tctcttctcc gccggccgcc ggttcatccg
    21901 accgaatcaa ctcgacgtcg aaatcgaagg aagtgcccag aatatttttc cccaacaatc
    21961 cgtatatccg agcatcccga atagcttgtt ccgttaatga aacagctttt tcgttggcgg
    22021 aacgaataaa aatataacct ttttcggctc cgaccaaaaa cgccccgatg attaaacctt
    22081 ccaatacctg atgaggtgaa cccgcaagca attttccatc cacatccgca tcttccggtt
    22141 gacaatgtcc gatcgcaacc acatattttt catcttcgga ttgctgccgg caggtttcca
    22201 ttttttgcca aagcggagtc cccaaaccgt aatgactgcg caggttcgat tctcggattt
    22261 cattcaacga ctgattttcg gagctcttca gtactttgct taaagcctta taaccgccgg
    22321 ctgaaaaata gtccgccaac gaatccggtg cgacgtcata aaaatgttcc gtaagaattc
    22381 tggcttgctg tcgattaaag gggatctgat cttccaagag cattcgattc cctgtaaggg
    22441 gatcgctgaa cagcaaatcc gtcaagaccc ggccttccaa taatgttttt tggataattc
    22501 tttccgcgtc agccgaagta aagtcggtat aaaggattcc gcaaggttcc aacaacagag
    22561 acactccggg acaagcataa taggtcgcag cggcaatttt taccttgatt ttcagccctt
    22621 tttcatctat gacatcctga agagccgaag caaaagcttc cgcttcttcc ggggcggcat
    22681 gattgccgtt caatacgatg agttttggag caaaacgaat ttccgccgaa tgaagcagtt
    22741 gcgataaata ttcataacga aaacgatcga tacggctacc ggtcgccggc atttttaccg
    22801 attttttcgc ttgcgagtcg ggatgctcag ccagctccat taattgtgag acagtcagaa
    22861 attcggcctc ttccggctct tcttccgcct ccgatatccc tatcgctttc gcggaagctt
    22921 cggcctttgt cttttcggca taataaaaat cggaggcgga aatcaaggaa ctttcagtgc
    22981 catcatccaa gccccgcaga tgatttaatc ccagcatccg taaagcatcg gaacggatca
    23041 aagccaactt tcgcggctct aaaacaggca agaaagccgg taattgggat tggatcccat
    23101 ttcgcagatt ctcgtcggat tcggaatgat tatcagccgc caatatggct aattccgtca
    23161 aggttaacgt tttcggttct tcctgaactt tctcccgata aagcagccct tccaagacct
    23221 catttcgcag aatcgtccgc cggataatcg tttcggcatc atattttcgc actttcacat
    23281 acaatgcccg tgtcggaaga gtcatcacca aaggaccctg atctttaaat ccaccggcat
    23341 cgattttttt gatgtggatc cgggcattgg atcccagttt gtcctgcgcc tgcctgaacg
    23401 cataatacaa atccatcgct tccgaggcac atttaccctc ccaagcacaa ataatgatat
    23461 tcggcttgga agaatcatat tttgcggtaa tcttttcaaa cgcctgttcc aatgcgatcc
    23521 gagtgctgga tttcgttttt tctttcgtgc tttctttgcg accgaaaaga tttgcggttt
    23581 tttttgaagc cagcacatag gcacccagta tcgtatcccc cagagttgga gttttttccg
    23641 aaccggacat atttttgctt aatcgaaacc cttttgcgga tcgcctcgcc gtttggtttt
    23701 ccggcttcgc aaagagctgc cttgactcat tttccggatc gtttcggaaa aaatctccca
    23761 atacccgata atcctggatc acatgatcaa ccaggtccgg cgcatccgcc ggaacgacat
    23821 gggagatgat ttcatagctt ggcatcaaca ttacgatcgg tccctgatct tccaaatcgc
    23881 gtccgtcgat ttctttcagc tcaatccgca catccgcttc ttttcggcgg gctgtttctt
    23941 ccaaagccgt aaatacggca attgcatccg ctgcctgcgg tccatcccaa acacaaacga
    24001 tgaccgttgc ccgatcatgg tcgtattctg cccacaattt ctttttcagt ttctcaagag
    24061 cgttaaaagt gccatatcgc atttcgctca ttttcttgat tttcctccgg cgaaaaatac
    24121 aacagttagc gtccgaatcc cgctttcccg ttacggatat ttcaaccttt taatcttacc
    24181 ttaaaaggtt ccg
//
LOCUS       DAOSMM010000028        24192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2095_length_24192_cov_16.016779, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000028 DAOSMM010000000
VERSION     DAOSMM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 24192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24192
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2095_length_24192_cov_16.016779"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK898_05680"
     CDS             <1..507
                     /locus_tag="PK898_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19560.1"
                     /translation="YLAVVGLQTVWEIKIKHPTFQFLKNALLFLGIGSLLIGNGLWII
                     RNHPFEYVYFAPLFRKYSDEFSGDYWGIASRNLLEYIVQTDDRPQIIVDHSLTNAGSI
                     NRGLLPEKDRDRLDLVYDTENADYIIFSRDDKTADQASFTGFKKVYSIRVDSDEIGIV
                     FKRETEIN"
     gene            513..1205
                     /locus_tag="PK898_05685"
     CDS             513..1205
                     /locus_tag="PK898_05685"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOU19561.1"
                     /translation="MPICYIVGAAEMDQTFHPEETDFTIAADAGLRHLRRLGIQPDLI
                     IGDFDSLGSVPPQPQVVCFPPEKDDTDMMLAVKAGLKKGYTDFRLYGGLGGRLDHTLA
                     NIQALLYLAHHQAQGFLIGEGMVLTAITNGSLRIKEGKKGILSVFCFGEPATGINLTG
                     LKYPLHDATLTSDMALGISNEFTDNPAEISVKNGTLVILWFDERFDDQSVSIIRFPRY
                     KDYSSASSFRTL"
     gene            complement(1264..2523)
                     /gene="ahcY"
                     /locus_tag="PK898_05690"
     CDS             complement(1264..2523)
                     /gene="ahcY"
                     /locus_tag="PK898_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOU19562.1"
                     /translation="MTDFDIKNDSLAEGGRRRIEWAEREMPVLRLIKERFQKEKPLKG
                     IRMSACLHVTAETANLMKTLQAGGADVVLVASNPLSTQDDVAAALVIHDEIPVHAIKG
                     EDNATYYKHIEAALAHKPQITMDDGADLVSTIHKSHRDLIPNIYGGTEETTTGVIRLR
                     AMAADKALMFPVIAVNDAMTKHFFDNRYGTGQSTLDGIIRATNILIAGKVFVVCGYGW
                     CGRGLAQKARGMGADVIVTEVDSLKALEAVMDGFRVMPIKEAAKAGDIFCTVTGDLNV
                     IDKEHFESMKDGAIVANSGHFNCEINIPALESLSVGKKLVRPFVEQYELKDGRKIHIL
                     AEGRLINLSAAEGHPASVMDMSFANQALSAEYMVKNHLTLEKKVYSVPEEMDREIAAL
                     KLKAMGVRCDELTEEQKNYLNSWESGT"
     gene            complement(2686..3690)
                     /locus_tag="PK898_05695"
     CDS             complement(2686..3690)
                     /locus_tag="PK898_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU19563.1"
                     /translation="MKRISILICSVLLLTAMVLSGCSTNTEKATATVKATVESVATVS
                     KVETAKDTASEAATTEETAATGSAVTTDETVKETAVTFSKENPITLKIGASPVPHAEI
                     LAQIKEPLAEKGINLEVVEFTDYIQPNIALEDGSLDANFFQHFPYLDDFNKNQKKENA
                     DWTTLVPLVYVHFEPMGIYKGKTASLDALKEGAKIAVPNDPTNEARALLLLEANGLLK
                     IAEGKGLEATAKDIVDNPKKFEIVELEAAQVALSIQDVDIAVVNGNYALSSGLTQADI
                     LAQESSDSEAAKKYANLVAVREGEEDLPQFVLLKEAITSDAVKKFIEEKYKGSVAPVF
                     "
     gene            complement(3776..4465)
                     /locus_tag="PK898_05700"
     CDS             complement(3776..4465)
                     /locus_tag="PK898_05700"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOU19564.1"
                     /translation="MSFFTYLINLLSEFHQLLIDATLQTLYITFISTVLAYLLGTPLG
                     ILVVITAPKSIAPHKGVNEVLSWIINIGRSIPFIILMVALMPLTRWIVGTTIGPVATI
                     VPLTIAATPFVARMIEQSLVEVDSGLIEMAQAMGATSSQIIFKVLLPEGLPSVVRGIP
                     ITIINLIGSYAMAGAIGGGGLGDLAIRYGYHRRQSDIMIVTIVLLIIIVEIIQLLGNF
                     VANRIDKRDVR"
     gene            complement(4470..5252)
                     /locus_tag="PK898_05705"
     CDS             complement(4470..5252)
                     /locus_tag="PK898_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU19565.1"
                     /translation="MSKQFEMKDGPFQALEDVSLHVEQEDIYGIIGMSGAGKSTLIRC
                     INLLEEPTWGEILIEGKCIFRRLKDVSDPKAEDQKFVRLNDTDLRKLRREIGMIFQSP
                     NLLMQRTVEENIAFPLEIAGYPRSKIQDRVKELMDLVNILDKAHNYPSQLSGGQRQRV
                     SIARAMANTPKILLCDEPTSALDSLTTTQILDLLWDINQQYKVTIVIITHEIELVKKL
                     CSKVAVIDNTRIVDQGEVGQVLLNPSMEITRQLMRGKIGSEE"
     gene            5736..6083
                     /locus_tag="PK898_05710"
     CDS             5736..6083
                     /locus_tag="PK898_05710"
                     /inference="COORDINATES: protein motif:HMM:NF023127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU19566.1"
                     /translation="MDKINENEKEYRFGDWGPKYIIKGPRMNMGIIRLKPGQKLTPHF
                     HKVMEENFYVEKGNVQLNVDGTVYNLIPGDVVRCEPNEVHALANVSSEEARVIFMLAP
                     YMESDKFEVEETK"
     gene            6435..7253
                     /locus_tag="PK898_05715"
     CDS             6435..7253
                     /locus_tag="PK898_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOU19567.1"
                     /translation="MIHTTITAHSGCDDTPMNSLESVLSGINDRADIIEVDVRADKNG
                     RLILSHNLDQERIYAGHAELIQVLEIFRTNKNICLNCDIKETEIIPSVLESAEQYQLS
                     KDRIYLSGSVTPELLRQRPEIIEKCDIQWNIEEAMIPLVEKKLIAEGKEYLRETLHTN
                     PWEVLRDEVPDPDRYLPDLIKMAKDFSLTTFNMPLEYATDDRIRFFHNNGLKVSVWTV
                     NDGEEIYRLLRLRVRNITTKKVKMAIKIKMDFLGFIEQEGRQKGDNRKNEFPEP"
     gene            7234..8073
                     /locus_tag="PK898_05720"
     CDS             7234..8073
                     /locus_tag="PK898_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU19568.1"
                     /translation="MSFLNPKRWYHSLGKRRFYEALLLIVFILFFYGPLLYTFMLAFA
                     NKYEYPSIIPSQFGFQWWNYVLNQPTLVKSIGLSFEFAFATTFISMIICLPAAYVLAR
                     YNFPGRKMVMFSFLLGNAFPKIGIYTSMAIVFYKMNLMGTFTGVLLIHLIECMMMMIW
                     LPTSAFRNVPANQEEAARDAGAGPLRTFWYVTLPTAWPGIAVAAMFAFIASLGESTGT
                     LLVGLPYYRTMTVEMYGVILDYPSTAGAVFSLILIAPNLALLLLMRRYLGADTLARGY
                     KMG"
     gene            8107..9120
                     /locus_tag="PK898_05725"
     CDS             8107..9120
                     /locus_tag="PK898_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19569.1"
                     /translation="MSDQVKLEIRNMTKLYKTGDGVRNINLTVNLGELVTLLGPSGCG
                     KTTILRTVAGFLSCDTGSILIDGKDISHLPPEKRPTAMVFQSYNLWPHMTVYENMAFG
                     LKLRKIPKPEIQRRITEMLELVKMSGFEKKYPNQMSGGQQQRIAIARSLLLQPEVLLL
                     DEPFSALDAKIRSQMREELRKIQTDLEITVVFVTHDQEEAMALSDRIVVMNKGSFEQI
                     GTPAEIYDHPASRYIADFIGEMNFIEQRDGTTIAVRPENVTLELGDGTGGDIGGYVRT
                     IMMLGHFYEIAIGTDSGIVKAYVSAETAGKYKRGDMVNCYFEGTYRYEPDSEKKKEKI
                     APA"
     gene            9206..10339
                     /locus_tag="PK898_05730"
     CDS             9206..10339
                     /locus_tag="PK898_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU19570.1"
                     /translation="MKTKLTLLLVLIFVVAVSPVAAADKPTVTLWSTGSQNVSDLFNN
                     LIEAYNARPESTSVVKLQFILSGTGEDTLSSRIAAAYKTGKTNSGFDIIAENSTSLQQ
                     YIDEAGSTDLFRDLDFSKIPNYKNLLIKPSIYESKFVPYRGTTVVMAYDSARLPNPPK
                     TWDELEAYVQANPGKFAYNSPSTGGAGSGFVRTAIYKFLPEESYMSNDPKWAEQYDQG
                     FALLKRIHPYLYKSGGKVVYPNKNQGTLDLLINKEVDLIPAWADQTLSNLANGTLPDT
                     VKIYQLEDYPLGGTDVVMAVTSIGADPEACYDFINYVISPEGQKKCLELMYAVPVIDI
                     STIQSDAAKMIEGLDVSSFKVFSIGSLGDVINQRWDREIATLP"
     gene            10446..11300
                     /locus_tag="PK898_05735"
     CDS             10446..11300
                     /locus_tag="PK898_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU19571.1"
                     /translation="MEKRTWKTNLFIVAMLAPSLLISLGVLVYPLLNTVVQSFKSPDT
                     GKYTLENYVYLFTDEISTASIVYTFWITVVTVIVTIAISYLLALYLRFTDNKVSKFIG
                     TIYLLPRFIPSLVAVYAMTTIIRDSGLLNRISLLFGNNFKPGLLYNAKGIILMNIWFN
                     IPFATMIIVSALSGIQDSVIESARDVGANQIRVFFSMILPLSIKDVMIAMTFVFMGNI
                     SSFTTPYLIGPNHPLMLGVYLRRLFSIYHEYELAAATSVVIFLFSSVSAIIYLYTNLK
                     EREWEARN"
     gene            complement(11302..12012)
                     /locus_tag="PK898_05740"
     CDS             complement(11302..12012)
                     /locus_tag="PK898_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOU19572.1"
                     /translation="MLEELKKSVLDANLSLPRYGLVTFTWGNVSGINRELGLVVIKPS
                     GVTYDAMSVDDMVVIELKTGKIAEGRLNPSSDTPTHLILYQAFPEIGGIVHTHSRWAT
                     IFAQMGQGIPPFGTTHADYFYGEIPCTRKMKNEEIQGAPGYYERETGNVIVERFKDID
                     PMMIPGVVVHSHGPFAWGENPQEAVHNAVVMEEVAFMAWHMMAVSAKPIPIMQQELLD
                     KHFLRKHGKDAYYGQKSG"
     gene            complement(12023..13504)
                     /gene="araA"
                     /locus_tag="PK898_05745"
     CDS             complement(12023..13504)
                     /gene="araA"
                     /locus_tag="PK898_05745"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOU19573.1"
                     /translation="MSELKNLEFWFVVGSQTLYGEEVLEIVAKRAREMAETLTNSPFI
                     PCKVVYKVTGKSPDEILQVMKEANFSDNCAGIITWMHTFSPSKMWINGLALLQKPYLH
                     LHTQYNRNIPNTEIDMDFMNLNQAAHGDREHGFIGARMRIPRKIVVGYWRDEKVQKEI
                     GSWMRSAVGYKISKNLKVMRFGDNMRNVAVTEGDKVETQIKLGWQVNTWTVGELANEI
                     EKVTPEEIDRLVREYESKYILNTDKTEPVRYQAREEIAIKRMLDREGASAFSNTFEDL
                     WGMEQLPGLATQHLMEMGYGYGGEGDWKTAAMVHIVKKMTEGIPGGTTFMEDYTYDLE
                     PGKELSLGAHMLEVCPSVAADKPRIEVHPLGIGNRKDPARLVFEGHEGPGIVVSLIDM
                     GGRVRLIVQDVEAIKPIYPMPNLPVARIMWKAMPDLLTGAHCWILAGGAHHTVFTYAA
                     TAEMMEDWADMMEIEFVHITKNTTVEALKHDLFLSDLAWKLRS"
     gene            complement(13501..15180)
                     /gene="araB"
                     /locus_tag="PK898_05750"
     CDS             complement(13501..15180)
                     /gene="araB"
                     /locus_tag="PK898_05750"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOU19574.1"
                     /translation="MNKYTIGIDFGTLSGRAVLVDVVTGEEVADSVYIYPHAVMDESL
                     PDGTPLGVDWALQDPQDYLEVYYHTIPELLKMSGVAAEDIIGIGIDFTACTFMPIKKD
                     GTPLCFLQEFQANPHAYVKLWRHHAAQDKANRLNQIAYERGEKWISRYGGKISSEWEV
                     PKVWQILDEAPEIYQATDCFIEGADWAVWALTGKLSRNTCTAGYKGIWHNKEGYPSKE
                     FFKALDPRLENYVEEKLNYPMVQLGTKVGGLTAEMAAKTGLLEGTAVAAGNVDAHVTV
                     PALKITGSGKMLAIIGTSTCHILLGDSEESVPGMCGVVEGGVYPGFYGYEAGQSCVGD
                     HFAWFTENCCPAEYTDQAEKEGKDIHAFLTEKAEKLAAGESGLVALDWWNGNRSVLVD
                     VDLTGMILGMTLATRPEEIYRALIEATAFGTRKIIDAFNENGVPVSEFYASGGISKKN
                     PLMMQIYADVIGLPIHIAGSAQGPALGSAIFAAVAAGKTAGGYDNVFAAANVMGKLDD
                     RIYIPDPENQAIYEKLYAEYSILHDYFGRGQNDAMKRLKQIKKDVKERQQQ"
     gene            complement(15218..16291)
                     /locus_tag="PK898_05755"
     CDS             complement(15218..16291)
                     /locus_tag="PK898_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HOU19575.1"
                     /translation="MDIKFARETSGKGKGGIMINRIKEKIDEKTYLLIITILMFIAMY
                     GAGMIIFADKGFAKPQMFLNLFVNNAGLIVTAVGMTMVMITGGIDISVGSVIGLTCMM
                     LAYMMEFMNVPAFLAILIVLVFGILFGSVQGFLIAYLKEQPFIVTLAGMFFCRGLTAV
                     ISSDMINISNATFLKIAQAKIYFPFLATVNKKGKVITPFIYPSVVIALIVLVVIYLVL
                     KYTKFGRAIYAVGGNQQSALLMGLNVRSTKMKTYVLNGFLASLGGVLFALNTCAGFVE
                     QGRGFEMEAIASAVIGGTLLTGGVGNVIGSLFGVLIKGTIESFITTQGTLSSWWTRIV
                     ISLLLAFFIVLQSIFASIKSKNR"
     gene            complement(16237..17277)
                     /locus_tag="PK898_05760"
     CDS             complement(16237..17277)
                     /locus_tag="PK898_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19576.1"
                     /translation="MKKTLKKITGFQLFLPIVALILVLLFNMIKTPDFFKITVLNGTL
                     YGFMIDIINRASELAILAIGMTLVVASSAGTDISVGAVSAVTAAIICFSLGPDQQNYQ
                     MPYLVGILLGLLGGVLCGMWNGLLVAKMRIQPMVATLILFTAGRGISQLICSGQIIHV
                     EKESYRILGSFIPKVPLPTPVFVAAALALVTWLVLKKTALGMYIQAVGINRRAGRLIG
                     INSQLIIFMTYVYCGLCSGIAGAIMSSRIAACDANNIGLNIELDAILAVALGGNSLGG
                     GKFSLLGSIIGAITIQALTTSLYAMHVTADQLPIYKAIVVVIIVTLQSAEFRKWISSL
                     QGKRPVKAKAAS"
     gene            complement(17261..18796)
                     /locus_tag="PK898_05765"
     CDS             complement(17261..18796)
                     /locus_tag="PK898_05765"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU19577.1"
                     /translation="MSNELLTMRHISKSFPGVVALNNVDFTLRSGEIHALMGENGAGK
                     STLIKVLTGVEELEEGEIRVSWLDHPLINHSPQEAQLNGISTVYQEVNLCPNLSVAEN
                     IFIGREPKKRGMIDWKAMNDNSRKILRSLNLSQVDVTKSLEHYSIAIQQMVAIARAID
                     IESKILIFDEPTSSLEEQETEKLFQIMNQLKEKGVGIIFITHFLEQVYEVSDRITVLR
                     NGELVGESLTKDLPRVQLVAKMLGKEFNDLAAIKKETKEKVNHTKGVPLIDARNLGHR
                     GTIKPFSLQMYPGEVVGLAGLLGSGRSELARTIYGADKPDSGELFVNTKKVKINAPID
                     AMKERMGYLPEDRKEEGIINDLSVKENIFIALQARQGMLKPLSNKEQTQLAEKYIKLL
                     NIKVANIDSPVKQLSGGNQQKVILARWLSTQPDFLILDEPTRGIDVGTKTEIQKLVLN
                     LAEEGMAIMFISSEIDEMIRTVSRMIVLRDREKVGELSEEEMTQDHIMAAIAGGERDE
                     EDA"
     gene            complement(18885..19853)
                     /locus_tag="PK898_05770"
     CDS             complement(18885..19853)
                     /locus_tag="PK898_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19578.1"
                     /translation="MKKVISLLVFVLLLSGVFAVAVSAADDLIVVGYSQVGAESDWRT
                     ANTESFKSTFTEENGYKLIFDDAMQKPENQIKAIRNFIQQEVDYIVLAPIVETGWETV
                     LGEAKAAGIPVILSDRMIKVTDDSLYVAWVGGNFIKEGHNAIEWLDNYLKENGRDNED
                     INIVLLQGTIGSSAQIGRTQGVEELLADRPKYHLLAKQTGEFTQAKGQEVMESFLKSY
                     PDIDVVIAENDDMGYGAMEAIRAAGKVPGKDIIMISYDAGRRAFEAILKGDLNATIEC
                     NPLHGPRVAEIIQKLEKGEPVEKIQYVDEGVFDIKNAAEVLPTRAY"
     gene            complement(20039..21043)
                     /locus_tag="PK898_05775"
     CDS             complement(20039..21043)
                     /locus_tag="PK898_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19579.1"
                     /translation="MIKKQILSYTVFLVSLCLAGCFYRPNAQRSSLVTPTESNLIVVG
                     YAQVGAESDWRVANTQSFKSTFTEENGFELIFIDAQQKPENQIKALRNFIQQEVDYIV
                     LAPIVESGWDSVLKEVKEAGIPLILSDRMVNVEDDSLYVSWVGGNFVKEGEDSVEWLH
                     NYLKEIGRDKEIINIVEMQGTLGSTAEIGRTEGIEKKIAEYPNFRLLEKQTGDFVQSM
                     GQEVMEGFLKKYDDIDVLIAQNDNMAFGAIDAIEAAGLVPGKDIIIISIDAVKAALEE
                     IVAGRINASIECNPLHGPRVAEIIRKLEAGEPVEKKIYVNEEIFDIKNAAERLPTREY
                     "
     gene            complement(21040..22425)
                     /locus_tag="PK898_05780"
     CDS             complement(21040..22425)
                     /locus_tag="PK898_05780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOU19580.1"
                     /translation="MTIIMLTMTLIIVVMLAVLIRSTNQYNLIVQNLVTASEFNFDFK
                     ETLDYKMYQYVIGGGDDFDTLDPLSDVSNALNVVEKLKLTTVRADNIKRLQTISAFLM
                     NLRTKIEKIHETHSYQENMTSVDYDIRVLTELIQEKVQEYIFYETNELAVIRERTNQE
                     ITRTIVILGIVSIGIVILLWIFSFRISDSIVRPIRMLSENFKMVGRGDFTVRSEKGYD
                     DEIQMLNDTFDSMVGQIEILLNDVKHEQENLRQTELKLLQAQINPHFLYNTFDTIIYL
                     AEDHQDEKVIEMITSLSVFFRTTLSKGEDIISLREEEVHACSYLEIQKVRYRDILEYE
                     IVIPEELLDYSVPKLTLQPLVENALYHGIKNKRGMGKITVKAWQSQCELFLQVKDNGI
                     GMKQENLEKLRKSVLEEHKIGFGLSNVHERIQLYYGSEYGLSIESEYDLGTEVTIKLP
                     LNLIRSEEQKI"
     gene            complement(22488..24122)
                     /locus_tag="PK898_05785"
     CDS             complement(22488..24122)
                     /locus_tag="PK898_05785"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU19581.1"
                     /translation="MLKVFLVEDEIIVREGIRDRINWVENGFEFSGEASDGELAYPMI
                     QQIKPDIVITDIKMPFMDGLALSRLIRNELPWTKIIILSGYDEFQYAKEAISIGVTDY
                     LLKPIGSAKVLETLLKIRDTIEEERNLREMQIRYRKEMQENENYQRRQFFSQLISDST
                     SVPEILNRANELDIDLAAKSYNIILFICSQKDESSWEKYSGKLVQMEEKIENNFSMRK
                     HMILFRRGLEGWALLIKGTDHESAESVTKRCIVELENLFQSDQSMDYFIGVGMPVERL
                     QQIPQAFQKANRAFVYRYIVEDNKIEYYSFDPKPAITSQNGFSLKLLDPSKLSQSVID
                     RFLRSGLKENVSQFVEDYIESIGLDSGNSLPFRKYIVTNILIGVSAFLDEIGIGKHVI
                     IDSFGDIEQITEKVTTVSYTKELIEKIFDVALDFRDNNSKQKYANLLSAAETYIRENY
                     NSEDISLNLVAQVTNVSPSYFSSIFSQEKGITFIEYLTNVRIEKAKELLRSTTLRSSE
                     ISQAVGYKDSHYFSYLFKKETGLTPREYRTGKEENN"
BASE COUNT         6654 a         5667 c         4814 g         7057 t
ORIGIN      
        1 tatctggcag ttgtcggtct tcagacggtt tgggaaataa aaatcaaaca tccgactttt
       61 caattcctaa aaaatgcttt actcttctta ggtatcggat cattgctaat cggcaacgga
      121 ctatggatca tacgaaatca tccttttgaa tatgtatatt tcgctccgct tttccgcaaa
      181 tactccgacg aattttcggg agattattgg ggaattgcca gtcgcaatct gttggagtat
      241 atcgttcaaa cggatgaccg tcctcagatc atcgtcgatc acagtttgac aaatgccggc
      301 agtatcaatc gcggactgtt gcctgaaaag gatcgagacc gtttggattt ggtttatgat
      361 accgaaaatg ccgattatat tatcttcagc agagatgata aaaccgcgga ccaagcatcg
      421 tttacgggat ttaaaaaagt ctattcgatt cgagtcgatt ccgacgaaat cggaatcgta
      481 ttcaaacggg aaacggaaat caactaaatc ttatgcctat atgttacatt gtcggtgcgg
      541 ctgaaatgga tcaaacattt catccggagg aaacagactt caccattgca gccgatgcgg
      601 gcttgaggca tttacggcga ttggggattc aacccgatct gatcatcggc gattttgatt
      661 cactgggttc ggttccgccg caaccccaag ttgtctgttt cccccccgaa aaagatgata
      721 cggatatgat gttggccgtc aaagccggtc tcaaaaaggg gtatacggat ttccgccttt
      781 acggcggttt gggcggcaga ttggatcata ctctcgcaaa tatccaagct cttctatatc
      841 ttgctcatca ccaggcacag ggatttttaa tcggcgaggg tatggttctg acggcaataa
      901 ccaacggttc acttcgtatc aaagagggca agaaagggat cctatccgtt ttttgtttcg
      961 gggagcccgc cacaggtatc aatctgacag gcttaaaata tccgctgcat gatgcaacgt
     1021 tgacaagtga tatggctctc ggaatcagca atgaatttac ggacaatccc gcggaaattt
     1081 cggttaaaaa cggcactttg gttattcttt ggttcgatga gcgtttcgat gatcagtcgg
     1141 tttcgatcat tcgctttccg agatataaag attattcatc cgcttcatcc ttccgaaccc
     1201 tttaaatgaa taaacctgag gaattcctca ggtttatcta tgcgaaatga ataatactgt
     1261 gttttatgtt ccggattccc aggaattcag ataattcttt tgttcttcgg ttaattcatc
     1321 acaacgcacg cccattgctt tcagtttcag cgccgcaatt tcgcgatcca tttcttccgg
     1381 aacagaatag actttctttt ccagagtcag atgattttta accatatatt ccgccgacag
     1441 agcttggtta gcaaaagaca tgtccattac cgacgccgga tgaccttccg ccgcagataa
     1501 attgattaat cttccttctg ctaaaatatg aatttttctt ccgtccttca attcatattg
     1561 ttcaacaaat gggcggacca gcttctttcc gaccgataaa ctttcaagtg cggggatatt
     1621 gatttcgcaa ttaaaatggc ccgaatttgc gacaattgcc ccgtctttca tcgattcgaa
     1681 atgttcctta tcaataacgt tgagatctcc cgttacggtg cagaaaatat cgccggcttt
     1741 tgcagcttct ttaatcggca tgacccggaa accatccatt accgcttcca aagctttcaa
     1801 agaatccact tcggtcacta tgacatccgc acccataccg cgtgccttct gtgcaagtcc
     1861 cctgccgcac caaccataac cgcacaccac aaaaactttc ccggctatca aaatattcgt
     1921 cgcgcgaata atgccgtcca gagtcgattg cccggttccg taccggttat caaagaaatg
     1981 ttttgtcata gcatcattga cagcgataac cggaaacatc aacgctttgt cggcagccat
     2041 agctcgcagc cggataacgc cggttgttgt ttcctccgta ccgccgtaaa tattcggtat
     2101 taaatcacga tgacttttat gaatcgtgct gaccaaatca gccccgtcat ccatcgtgat
     2161 ttgcggttta tgagccaaag cagcttcgat atgtttatag taggtggcat tatcctcacc
     2221 tttgatcgca tgaaccggaa tttcgtcatg gatgaccaaa gcggcggcca catcatcttg
     2281 tgtggataac ggatttgatg cgaccagcac cacatccgct cctccggctt gcaaagtctt
     2341 catcaaattt gcggtttccg ccgtaacatg cagacaagct gacatccgaa ttcccttgag
     2401 cggtttctcc ttttggaatc gttccttaat caaacgaagg accggcattt ctctctctgc
     2461 ccattcaatc cggcggcgtc cgccttccgc caacgaatca tttttgatat caaaatcagt
     2521 catgaaagct ccttaattgt tttattgaca atagatttta ctatgattta cgttcaagtt
     2581 tccgtaaaag gctcaatcga attattgaaa tcggtttttt gctttaaaca aaacggccgg
     2641 tctttccaaa ccggtcgttt tattggttca aattcttgcg ataaatcaga atacgggagc
     2701 gacggaacct ttatatttct cctcaatgaa tttcttcacg gcatctgaag tgatcgcttc
     2761 ttttaacaat acaaattgcg ggagatcctc ttcgccttcg cgaaccgcaa ccaaattggc
     2821 atatttttta gccgcttcgg aatcggaaga ttcttgagca aggatatccg cttgggttaa
     2881 gccggaactt aatgcatagt tgccgtttac aaccgcaata tcgacatctt gaatggacaa
     2941 ggccacctgc gcggcttcca attcgacgat ttcaaacttc ttgggattgt cgacaatatc
     3001 tttcgccgtg gcttccaaac ctttcccttc cgcaattttg agcagaccgt ttgcttccag
     3061 taggagcaaa gcccgcgctt cattggtggg atcattcgga acggcgattt tcgcgccttc
     3121 ttttaaggca tccaaactgg cggtcttccc tttataaatg cccatcggtt caaaatgtac
     3181 gtaaactaac ggaacaagag tggtccaatc cgcattctct tttttctgat ttttattgaa
     3241 atcatccaga taagggaaat gctggaagaa attagcatcc agactgccgt cctcaagagc
     3301 aatattcggt tgaatatagt ccgtaaattc aaccacttcc agattgatcc ctttttcggc
     3361 taacggctct ttgatttgag ctaatatttc cgcatgcgga accggtgatg caccgatttt
     3421 aagggtgatc ggattttctt tggaaaaagt aaccgctgtt tccttaaccg tttcgtcggt
     3481 tgtcactgcc gaaccggttg ctgcggtttc ttctgtcgtt gctgcttcgg aagcagtatc
     3541 ttttgcggtt tcaactttgg aaacggttgc gacactttca accgtggctt tcacggttgc
     3601 cgtcgctttt tccgtattcg ttgagcaacc cgacagaacc attgctgtta acagcaatac
     3661 actacaaata agaatgctaa tacgtttcat gatattgctc cttctaaaac aaattcactt
     3721 atttgaataa gaaagatcga acggaaatcc gctctctctt ttttcgtaca acagatcaac
     3781 ggacatcccg tttatcaatc cgattcgcta caaagttacc cagcaattga atgatttcca
     3841 cgatgataat caacaagact attgtgacaa tcatgatatc agactgccgg cggtgatatc
     3901 cgtatcggat cgccaaatct cccagcccgc ccccgccgat ggcaccggcc atggcatagg
     3961 atccgatcaa attgataatg gtgatgggaa tgccgcgaac caccgaaggc aatccttccg
     4021 gcaatagcac tttaaagata atctgagagg aagtggcgcc catggcttga gccatttcaa
     4081 tcaagccgga atccacttcg accaaggatt gttcgatcat gcgcgcgaca aaaggcgtcg
     4141 ccgctattgt gagcggaaca attgtcgcaa ccggcccgat tgtcgttccg acgatccaac
     4201 gggtaagcgg catcagagcg accatcagga taatgaatgg aatggatctg ccgatattaa
     4261 tgatccagct gagtacttca ttaactccct tatggggagc gatcgattta ggcgccgtaa
     4321 tcacaactaa gattcccagc ggggtgccca acaaataagc cagaaccgta gaaatgaatg
     4381 ttatatataa agtctgtaac gttgcgtcga ttaataattg atgaaattca ctcaacaagt
     4441 tgataaggta agtaaaaaat gacatagcgt tattcctcac tcccaatctt gccgcgcatc
     4501 aactgccggg taatttccat gctcgggttc aacaaaactt gtccgacttc cccttgatcc
     4561 acaattcgag tattatcaat aaccgctact ttgctgcata atttcttaac taactcaatt
     4621 tcatgtgtga taatgacgat cgtcacctta tactgttgat taatatccca caataggtcc
     4681 aagatctgtg tggtggtcag cgaatccaat gcggaagtcg gctcatcgca caaaagtatt
     4741 ttcggggtat tcgccatcgc tcgcgctatt gaaacccgct gccgctgccc tccggatagc
     4801 tgagacggat aattgtgagc tttatccaga atattcacca aatccatcag ttcctttacg
     4861 cggtcttgaa tcttcgatcg cggatagccg gctatttcca gcggaaaagc aatattttct
     4921 tccacagtcc gttgcatcag aagattcgga gattgaaaaa tcatcccgat ttcacgccgt
     4981 agtttgcgta aatcagtatc attgagtcga acaaattttt gatcctccgc tttcgggtcg
     5041 ctgacgtctt ttaaccttct gaaaatacat ttcccttcaa tcagaatctc tccccatgtc
     5101 ggttcttcca gcaaattgat acagcggata agcgtgctct tgcccgctcc gctcatgccg
     5161 ataataccgt aaatatcttc ctgttctaca tgcagagaga catcttccag tgcctgaaaa
     5221 ggaccatcct tcatttcaaa ttgtttactc aagtggcgga tctcaatcat gcttatgaaa
     5281 ccttatttac ttatttttca aaaaaaaggc tcttttacga tgaaaagagc ctcacgaatc
     5341 agacataatt atgaagataa agaaaagact atctttgata cgttaagctc aaccgccccg
     5401 tatggcggcc ggccatcaac attccgtacc cgaaatagat catttttatc ataaggctag
     5461 tttagtagaa atcgaattac cggtcaaggg attccttcat ttttatgaca tttaatcatc
     5521 gtttacggtc aaagaatgat ctctttaata atgtgtttaa ttatcagatg atcaaatatc
     5581 ctatcaccca aaatcctctc aagattcaaa aattcagaga acagtctgct ttaactttga
     5641 taaaattata ttgacatttt ttacacggaa acaaaaattt ttttatagga tatgaattcc
     5701 atctcaaacg aatttaaaaa ggggtatcaa ggcatatgga caagatcaat gaaaacgaaa
     5761 aagaataccg attcggtgat tggggaccga aatatatcat caaagggccg cggatgaaca
     5821 tgggaattat tcgtttaaaa ccgggacaaa agttgactcc gcattttcat aaagtgatgg
     5881 aggaaaattt ttatgtcgaa aaaggaaatg ttcaattaaa tgttgacggg accgtttata
     5941 acttaatacc gggggatgtc gttcgttgtg agccgaatga agttcacgca ctcgctaacg
     6001 taagttccga agaagcccgt gtaatcttta tgttagcgcc ttacatggaa tcggataaat
     6061 ttgaggttga agagacaaaa taagctgccg taagattgtc cgaatcggca tttttgcaga
     6121 aatgaaatga aacaatgata aagggagaaa ctatagggga taacctttct aaaaaggtaa
     6181 ttttcaggca aaacatctat tgtcttttac ccgcttttta cgacaatttt acagattaaa
     6241 tcctttcttt cggttgtccc atgttatgat tgtttttgtt tcaagaatca tatttccgct
     6301 ccgatcagcg ggaacaaatc gtttttagag aattttcaaa cagcttattg aagatgtatt
     6361 ttttgccata gaaaggtgag ttttcatttt ttatcggaaa aaattcgcac gagaatatgc
     6421 aaaacggaaa taatttgatt cacacaacga tcactgccca cagcggttgc gatgatacac
     6481 cgatgaattc gctggaatcc gttttatcgg ggataaatga ccgagcggat attatcgaag
     6541 tagatgtccg agccgataaa aacggcagat taattctctc tcacaatctt gatcaagaga
     6601 gaatatatgc cggacatgcg gaattaatcc aagttttgga gattttccga acaaataaaa
     6661 atatttgctt gaattgcgac atcaaagaga cagagattat tccttcggta cttgaatcag
     6721 ccgaacaata tcagctttcc aaagacagaa tttatctttc cggcagtgtt acgccggaat
     6781 tattgcgcca acggccggaa ataatcgaaa aatgcgatat tcaatggaat atcgaagaag
     6841 cgatgattcc gttggttgaa aagaaactga ttgcagaagg gaaagagtat ttaagggaga
     6901 cgctgcatac caatccatgg gaagtcttac gtgacgaagt gcccgatccc gatagatacc
     6961 ttcccgattt gatcaaaatg gcgaaagatt tttctcttac tactttcaat atgccactcg
     7021 agtatgccac ggatgaccgc attcgattct tccataataa cgggttaaaa gtctcggttt
     7081 ggacagtgaa tgacggtgaa gaaatctaca ggctgctacg attaagagta cgaaatatca
     7141 ccacaaaaaa ggtaaaaatg gcaataaaga tcaaaatgga ttttctcggt tttatcgaac
     7201 aagaaggcag acagaaagga gataatcgga aaaatgagtt tcctgaaccc taaacgttgg
     7261 tatcacagtc tcggtaaaag acgtttttat gaagcacttc ttctgattgt ttttatcctg
     7321 tttttttatg gaccgttgct ctacactttt atgctggcgt tcgcaaataa atacgagtac
     7381 ccttcgatta tcccctctca attcggcttt caatggtgga attatgtact aaatcagccg
     7441 acattagtga aatcaattgg tctttcgttt gagtttgcgt ttgcgacaac gtttatttca
     7501 atgatcatct gccttcccgc agcttacgtt cttgcgagat ataatttccc cggaaggaaa
     7561 atggtgatgt tttctttcct gttgggaaat gcattcccga aaatcggtat ctacacttcc
     7621 atggcgatcg tgttttacaa gatgaatctg atggggacgt ttacaggtgt tctgctgatc
     7681 catctgatcg aatgcatgat gatgatgatt tggcttccga ccagcgcatt ccgcaatgtt
     7741 cccgccaacc aagaggaggc agccagggat gccggtgccg gtccgctgcg tactttttgg
     7801 tacgtaacgc tgccgactgc ctggcccggg atcgccgtcg ccgctatgtt tgcttttatc
     7861 gcctctttag gcgaaagcac cggcactctc ttggtgggtt tgccgtatta ccgcacgatg
     7921 accgtagaaa tgtatggcgt tattcttgat tacccatcta ccgccggcgc tgttttctct
     7981 ttgatcctta tcgcccccaa cctcgcattg ttattattga tgcgcaggta ccttggcgcg
     8041 gatacgctcg cccgcggata caaaatgggt taattcatca aaaaaccggg aaggaaactt
     8101 tacattatga gtgatcaggt aaaactcgaa atccgcaata tgaccaaact atataagacg
     8161 ggcgacggag tcagaaacat taatctgact gtaaatctgg gagaattagt cactttgctc
     8221 ggcccgtccg gttgcggaaa aacaaccatt ttacgcacag ttgccggttt tttgagttgc
     8281 gataccggtt cgattttgat cgacggtaag gatatttctc acttgccgcc cgaaaaacgc
     8341 cctaccgcta tggtttttca aagctataac ttatggccgc atatgaccgt ttatgaaaat
     8401 atggctttcg gcttgaaatt acggaaaatt ccgaagccgg aaatccaaag aagaattacc
     8461 gaaatgctcg aattggtaaa aatgtcgggc tttgaaaaaa aatatcccaa tcaaatgtcg
     8521 ggaggacaac aacaacggat cgcgattgcc cgttctcttt tgctccaacc ggaagtgctg
     8581 ctcttggatg aacctttttc ggcattggat gccaaaatcc gttctcaaat gcgtgaggaa
     8641 ttaaggaaga tccagaccga tttggaaatc acggttgtct ttgtcacgca tgaccaggaa
     8701 gaggcgatgg cgctgtccga tcggattgtg gtgatgaata agggatcttt cgaacagatc
     8761 ggaacaccgg cggaaattta cgatcatccg gcatcccgct atatcgccga cttcatcgga
     8821 gaaatgaatt ttatcgaaca gcgtgacgga acaacaatcg ccgttcgtcc cgaaaacgtg
     8881 acactggaat tgggggacgg aaccggcgga gatatcggcg gttacgttcg aaccattatg
     8941 atgttaggcc atttctatga aattgcaatc ggaacggaca gcggaattgt gaaagcgtac
     9001 gtatcggcgg aaacagccgg aaaatataaa cgcggagaca tggtaaattg ttattttgaa
     9061 ggcacttatc gttacgaacc cgattcggaa aagaaaaagg aaaaaattgc gcccgcataa
     9121 tcctgccggt gcaaatttat cattcgttga ataaccgcgc ttcccaattt tgggagccgg
     9181 aattaatcaa aaaaggagta ttttaatgaa aacaaaatta actttattgc ttgtgttgat
     9241 tttcgtagtc gctgtcagcc cggtcgcggc tgcggacaag ccgaccgtaa ctttatggag
     9301 cacgggatca caaaatgttt ccgatctctt caataatctt attgaggctt ataacgctcg
     9361 acccgaatca acctctgtcg taaaactgca atttatcctt tccgggaccg gtgaagatac
     9421 attaagttca cggattgccg cagcctataa gacgggtaag accaattccg gttttgatat
     9481 tatcgcggaa aacagtacct ctctgcaaca atatatcgat gaagccggaa gtacggattt
     9541 attccgggac cttgacttct caaagatccc caattataag aatcttctga ttaaaccttc
     9601 catttatgag agcaaatttg taccgtaccg cggaacaaca gtggttatgg cttatgattc
     9661 ggcacgactc cccaatcctc ctaaaacatg ggacgaattg gaagcatatg ttcaagcaaa
     9721 ccccggaaaa tttgcctaca actctccgag caccggcggc gcgggttcgg gctttgttcg
     9781 cacggcgatt tataaattcc tgccggaaga atcctatatg tcgaatgatc cgaaatgggc
     9841 ggagcaatat gaccaaggtt tcgctttgtt gaagagaatt catccttatc tgtataaatc
     9901 gggcgggaaa gtggtctatc ccaacaagaa ccagggaacg cttgacctgc tcataaacaa
     9961 agaggtggat ttgatccccg cttgggcgga tcaaacgtta agcaatttgg caaacggaac
    10021 tctgccggat accgtaaaga tttatcagct cgaagattat cctctcggcg gtacggatgt
    10081 agtgatggct gtgacttcca tcggcgccga tccggaagca tgttatgact ttatcaacta
    10141 cgtgatcagt ccggaaggtc aaaagaaatg cttggaactg atgtacgccg taccggttat
    10201 cgacatttct acgattcaat ccgacgccgc aaaaatgatc gagggtttgg atgtctcaag
    10261 ctttaaggtc ttctcgatcg gcagtctggg tgatgttatc aaccaaagat gggatcgtga
    10321 aatcgcaacg ctgccgtagc agatttgcta actctgtaat cgattgaagc gggtatggaa
    10381 tcatcattcc cgacaaagga aagaatgatt ccatacccaa agaatttctt agaggggaat
    10441 aaaacatgga aaaacgtact tggaaaacca acctttttat tgtcgcgatg ctcgcaccgt
    10501 cgttgctgat ttcgctggga gtgctggttt atcctttatt gaatacggtt gttcaaagtt
    10561 ttaaaagccc cgataccgga aaatacacgc tggaaaatta tgtttatctc tttacggatg
    10621 aaatttcgac cgcaagcatt gtttacacat tttggatcac ggtagttacc gtaatcgtaa
    10681 cgattgcgat ttcttatctt ttagcacttt atcttcgctt tacagacaat aaagtcagta
    10741 aatttatcgg tacaatctat cttttaccca gattcatccc cagtttggtc gccgtttatg
    10801 ccatgacgac gatcattcgc gattcggggt tgttaaatag aatatcactc ttattcggaa
    10861 ataattttaa accgggtttg ctctataacg caaaaggaat catcctgatg aatatctggt
    10921 tcaatattcc gtttgctacc atgattattg tctccgcgct ttctggaatt caggattcgg
    10981 tcatcgagag tgcgagagat gtgggagcga atcaaattcg ggttttcttt tcaatgattc
    11041 ttccgctgtc cattaaggat gtcatgattg cgatgacttt cgtttttatg ggcaatatca
    11101 gttcgttcac aacgccctac ttaatcggtc cgaatcaccc gttgatgctc ggcgtttatt
    11161 tacgaagatt attctccatc tatcacgaat atgaattagc cgctgccacg tcggtcgtca
    11221 ttttcctgtt ctcatccgtt tcggcaataa tttatctcta caccaatctc aaagaacgcg
    11281 agtgggaagc gagaaattag ctcaacctga tttttgaccg tagtaagcat ctttaccgtg
    11341 cttacgcaaa aagtgtttat ccaacagttc ctgttgcatg atcgggatag gcttagcgct
    11401 gaccgccatc atatgccatg ccataaaagc cacttcttcc attacaacag cgttatggac
    11461 agcctcttgc gggttttcgc cccatgcaaa aggtccgtgg ctatgtacga ccactcccgg
    11521 aatcatcatc ggatcgatat ctttaaaacg ttcgacaatt acgttgccgg tttcgcgttc
    11581 gtaatagccc ggcgcacctt gaatttcttc attcttcatt tttcgagtac agggaatttc
    11641 gccataaaaa taatcggcgt gagtcgttcc gaacggcgga attccctgcc ccatttgagc
    11701 gaatatcgta gcccaacgac tgtgcgtatg gacgattccg ccgatttccg gaaatgcttg
    11761 ataaagaatt aaatgagtcg gtgtatcgga tgaagggttc aaccttcctt ccgctatttt
    11821 tccggttttg agttcgatta caaccatatc gtcaacgctc atggcgtcat aagtcacacc
    11881 agacggttta atcacaacca gacccaactc tctgtttatg ccggagacat tcccccaggt
    11941 aaaagtgacc aaaccgtatc ggggcaatga caaatttgca tccaaaacag atttttttaa
    12001 ttcttcaagc atctcaaaaa tcctatgacc gcagtttcca cgccaaatca gataagaata
    12061 aatcgtgttt gagcgcctcg actgttgtat tttttgttat atgaacgaat tcaatttcca
    12121 tcatgtcagc ccaatcttcc atcatttcag ccgtcgcggc atacgtaaaa acagtatgat
    12181 gggcacctcc ggcaagaatc cagcaatgtg cacccgttaa taaatccggc atagctttcc
    12241 acataattcg agcgaccggc agattgggca tcgggtagat gggcttaatg gcttcgacat
    12301 cctgaacgat cagacgcact cttcctccca tatcgatcag cgaaaccaca attcccggtc
    12361 cttcatgtcc ttcaaaaaca agtcgggcgg gatcctttcg gtttccgatt cccaacgggt
    12421 gaacttcgat tctgggttta tccgccgcca cactcgggca cacttccaac atgtgcgctc
    12481 ccaaggagag ctctttcccg ggttccaaat cataggtgta gtcctccatg aaagtggtcc
    12541 cgccgggaat tccttcggtc atcttcttaa caatatgaac catggcggct gttttccagt
    12601 ccccttcacc cccatatccg tatcccattt ccatcaagtg ttgggtggca agccccggca
    12661 gttgttccat tccccaaaga tcttcgaaag tgtttgaaaa agcgctggcg ccttcccgat
    12721 ccaacatccg tttaatggcg atttcttccc gtgcctggta ccgcaccggc tctgttttat
    12781 ccgtatttaa gatatattta ctttcgtact cccgcaccaa tcgatcaatt tcttcgggag
    12841 tgactttctc aatttcgttc gccagttcac caactgtcca tgtattcacc tgccagccaa
    12901 gcttaatctg tgtttcgact ttgtcaccct cggtcacagc cacatttcgc atgttgtcac
    12961 cgaagcgcat gactttgaga tttttactga ttttatatcc gactgcggat cgcatccaac
    13021 tgccgatctc tttctgaact ttttcatccc gccaataccc gacaacaatt tttcgcggaa
    13081 ttctcatgcg ggctccgata aatccgtgtt cccgatcacc gtgagcagcc tgattcaaat
    13141 tcataaaatc catatcgatt tcggtattcg gaatattccg attgtattgt gtgtgaaggt
    13201 gaagataagg tttttgcaga agagccaggc cgttgatcca cattttcgac ggcgaaaagg
    13261 tatgcatcca ggtaattatg ccggcgcaat tatccgaaaa atttgcttct ttcatgacct
    13321 gtaatatttc atccggtgat tttcccgtca ctttgtagac cactttacag gggataaatg
    13381 ggctgtttgt cagagtctca gccatttccc gagctcgttt agccactatt tccaatactt
    13441 cctcaccgta cagtgtttgc gatccgacaa caaaccagaa ttcaagattt tttaattcac
    13501 tcattgctgt tgcctctctt tgacatcttt ttttatttgt ttcaatcgtt tcatcgcatc
    13561 attttgtccg cgaccgaaat aatcgtgcag gattgaatat tccgcataga gcttttcata
    13621 gattgcctga ttttccggat cggggatata aattcgatca tccagctttc ccattacatt
    13681 tgcagcggca aatacgttat catacccgcc cgccgttttt ccggctgcca ccgcggcaaa
    13741 aatcgcactc cccaatgcgg gaccctgagc ggatccggca atatgaatcg ggagcccgat
    13801 cacatcggca tagatttgca tcataagcgg attctttttg gagatccctc ccgaggcata
    13861 gaattccgac accggaaccc cgttctcatt aaatgcgtcg attatttttc gggtaccgaa
    13921 agccgtcgct tcaattaatg cccgatatat ttcttcgggt cgtgtcgcca atgtcatccc
    13981 caaaatcatc cctgttaaat cgacatcgac caaaaccgat cgattcccgt tccaccaatc
    14041 cagcgcgacc aaaccgcttt caccggctgc cagtttttcg gctttttccg ttaagaaggc
    14101 atggatatcc tttccttctt tttcggcttg atccgtatat tccgccggac aacaattctc
    14161 cgtaaaccaa gcaaaatggt ctccgacgca gctttgtccg gcctcatatc cgtagaagcc
    14221 cggataaacc ccgccttcca ccacaccgca cattccggga acggattctt cggaatcccc
    14281 caacaaaata tggcaggtag aggtcccgat gattgccaac atttttccgg aaccggttat
    14341 tttcaatgcc ggaaccgtaa catgggcatc cacattcccg gctgcgactg cggttccttc
    14401 gagtaacccg gtctttgcgg ccatctccgc tgtcaaccct cctactttag tccccagttg
    14461 aaccatggga taattcaatt tctcctcgac ataattttcc aaacggggat ctaaagcctt
    14521 gaaaaattct ttggagggat atccttcttt attgtgccaa atccctttat aaccggcggt
    14581 acaggtattt ctggataatt tgccggtcaa tgcccaaaca gcccagtccg ccccttcgat
    14641 gaaacaatcc gtcgcctgat atatttccgg tgcttcatcc aaaatctgcc atactttggg
    14701 gacctcccat tcggaggata ttttgccgcc gtagcgggat atccattttt ctccgcgttc
    14761 ataagcaatt tgattgagcc ggtttgcttt atcctgtgcc gcatgatgcc gccataattt
    14821 gacgtatgcg tgcggatttg cttggaattc ttgcaaaaag cagaggggcg ttccatcttt
    14881 ttttatcggc ataaaggtac atgcggtgaa atcaatcccg attccgataa tatcttccgc
    14941 agccacaccg ctcattttga gcaattcggg gatcgtatgg taatagactt ccaaataatc
    15001 ctgcggatct tgcaaagccc aatccacccc caacggagtc ccgtccggca aagattcatc
    15061 cataaccgcg tgtggataga tatagacact gtcagccact tcttctccgg tgacgacatc
    15121 caccaagaca gcccgtcccg aaagtgtacc gaaatcgatt ccgattgtat atttattcat
    15181 ttagacctct gacgtatttt gaaatattat cctttgatta tcgattctta ctttttatcg
    15241 aagcaaatat actctgaaga acaatgaaaa acgccagcag taaggatata acgatccttg
    15301 tccaccatga cgaaagggtt ccctgagtgg tgatgaacga ttcgatcgtt cctttaataa
    15361 ggactccaaa aagggagccg attacattgc cgactccgcc ggtcaaaagg gtgcctccga
    15421 ttactgcaga ggcaatcgct tccatctcaa aacctcttcc ctgttcgacg aatccggcac
    15481 acgtgttcaa agcaaaaagg actcccccca aagaggctaa gaagccgttt aaaacatatg
    15541 ttttcatctt ggtcgaacga acattcaagc ccattaacaa tgcggactgc tgatttccgc
    15601 ccaccgcata aatcgctctg ccgaactttg tatatttaag aaccaaataa atgaccacca
    15661 gcacaatcaa tgcgatgacg acactgggat agataaaagg cgtgattacc tttccttttt
    15721 tattgaccgt tgccaaaaaa ggaaaatata tttttgcctg agcaattttc agaaaggttg
    15781 cattcgagat attgatcata tccgaactga ttaccgccgt aagacctctg caaaaaaaca
    15841 tacccgctaa tgtgacgatg aacggttgtt ctttcaaata agcgatcaga aatccttgca
    15901 cggatccgaa tagaatcccg aataccaaaa cgatcaaaat tgccaaaaat gccggaacat
    15961 tcataaattc catcatatat gccagcatca tacaagtcag accgattacc gaaccgaccg
    16021 aaatatcgat cccgcccgtt atcatcacca ttgtcatacc gactgccgtg acgatcagcc
    16081 ccgcattgtt gacgaaaaga ttaaggaaca tctgcggttt tgcgaatccc ttgtcggcaa
    16141 aaatgatcat tccggctccg tacatcgcaa taaacatcag aatcgtaatg atcaacaaat
    16201 aagttttttc atcgattttt tctttgattc ggttgatcat gatgccgcct ttgcctttac
    16261 cggacgtttc ccttgcaaac ttgatatcca tttccgaaat tctgcggatt gcaaggtgac
    16321 aatgattacg acgacgatag ctttatagat gggcaattga tcagcagtta catgcattgc
    16381 ataaagagaa gtggtcagcg cctgaatcgt gatcgcaccg ataatggaac ccagaagcga
    16441 aaactttccc ccacccaggc tgttcccacc gagcgcgacc gctaatatcg cgtccaattc
    16501 tatattcaag ccgatattat tggcatcgca agcagcgatt ctcgaactca tgatggctcc
    16561 cgcgattccg gaacaaagtc cgcaatatac atacgtcatg aaaataatca gttgagaatt
    16621 gattccgatc agccttccgg ctctacgatt gataccgacg gcttgaatgt acattcccaa
    16681 agcggtcttt ttcaaaacca accaagtgac aagagccaaa gcagccgcga caaagacagg
    16741 agtcggtaac ggtactttcg gaatgaaact cccgaggata cggtaacttt ccttttcgac
    16801 atgaatgatc tgtccggaac agattaattg ggatattccg cggcccgcgg taaaaagaat
    16861 taatgtggcg accatcggtt gaattctcat tttcgcaacc agcaaaccgt tccacattcc
    16921 gcataaaacg ccacccaata atcctaacaa aatcccgact aaatatggca tctgataatt
    16981 ttgttgatca ggtcccaagg aaaaacagat aatcgcagca gtaactgcgc taaccgcacc
    17041 tacggaaata tcggtaccgg cggaagacgc tactactaag gtcatcccga ttgcgagtat
    17101 cgccaattca ctggctcgat taatgatatc gatcatgaat ccgtacaatg tgccgtttaa
    17161 aacagtgatc ttgaaaaaat cgggggtttt aatcatgtta aacagcaaaa ccaggattaa
    17221 agccacgatc ggtaaaaaca gctggaaacc ggtaattttc ttaagcgtct tcttcatctc
    17281 tttcacctcc ggcgattgca gccataatat gatcttgagt catttcctct tcgctgagtt
    17341 ctccgacctt ttctctgtct cttaatacaa tcattcggct gacagtacga atcatttcat
    17401 caatttccga agaaataaac atgattgcca ttccttcttc cgccagattc aacactaatt
    17461 tttgaatttc ggttttcgtt ccgacatcaa ttccccgtgt cggttcatct aaaatcagga
    17521 aatcaggctg cgttgacagc caacgtgcaa gaatgacttt ctgttgattt cctccgctga
    17581 gttgcttaac gggactgtcg atatttgcga ctttgatatt cagcaattta atatattttt
    17641 ccgccagttg cgtttgttct ttgttgctca acggttttaa catcccctgg cgagcttgaa
    17701 gtgcaataaa tatgttttct tttacggaaa ggtcattgat gatcccttct tctttgcgat
    17761 cttccggcaa ataccccatc cgttctttca tcgcatcgat cggggcgttg attttcactt
    17821 ttttcgtatt gacaaataat tctccgctgt ccggtttgtc tgcgccgtaa attgtccggg
    17881 caagttcaga gcgtcccgag ccgagaagtc ccgctaaccc cacaacttca ccgggataca
    17941 tttgtaagct gaacggttta atcgttcctc tgtgccccag atttctcgca tcgatcagcg
    18001 gcacaccttt cgtatgattc accttctctt ttgtctcttt cttaatcgcc gccaaatcat
    18061 taaattcttt tcccaacatt ttagcgacca gctggacacg cggtaaatct ttcgttaaac
    18121 tttcgccgac taattcaccg tttcttaaaa cggtaatccg gtcggatacc tcataaacct
    18181 gttccaaaaa atgagtaata aaaatgattc ccaccccttt ttccttcagt tgattcatga
    18241 tttgaaacaa tttttcggtt tcttgttctt ccaatgaaga ggtaggctcg tcaaaaatca
    18301 gaatttttga ttcaatatca atcgcccggg caatcgcaac catttgttgg atggcgatag
    18361 aataatgttc caaggatttc gtgacatcga cttgtgaaag attaaggctt ctgaggattt
    18421 ttctggaatt atcattcatt gccttccaat cgatcatgcc tcgctttttc ggttcgcgac
    18481 cgataaaaat gttttcggca accgaaagat tgggacataa gttcacttcc tgataaacgg
    18541 tgctgatccc gttgagttgc gcttcctgag gggaatgatt gattaacgga tgatccaacc
    18601 aagagacacg gatttcacct tcttcgagtt cttcgacacc cgtcaaaact ttgatcaggg
    18661 tagactttcc ggctccattt tcgcccatta aagcatggat ttctccgctt cggagagtaa
    18721 aatcaacatt attcagcgct acaactccgg gaaaagattt actgatatgc ctcattgtta
    18781 gtagttcatt gctcatattt aaattcccaa agtggttttc aaattcgttt gaaaaaaatg
    18841 gcaggtggtc tttcccgcct gccatttttt cgaacactca aagactaata agcccgtgtc
    18901 ggaagaactt cagctgcatt cttgatatcg aatacgcctt catctacgta ttgaatcttc
    18961 tcaaccggtt cacccttttc cagtttctgg ataatttcag cgacacgcgg accgtgaagc
    19021 gggttgcatt cgatggtcgc attcagatca cctttcagga tggcttcaaa agcccgacga
    19081 ccggcatcat aggaaatcat gatgatatct tttccgggga ctttaccggc agcccgaatg
    19141 gcttccatcg caccgtatcc catatcatca ttctcagcga tgaccacatc gatatccgga
    19201 taggatttca ggaatgattc cataacttcc tgacctttgg cctgagtaaa ttcacccgtc
    19261 tgtttggcaa gcagatgata ttttggccga tcggcgagta attcttccac gccctgtgta
    19321 cggccgattt gtgcagaaga accgatcgtc ccttgaagca gaacaatgtt gatatcttcg
    19381 ttatctcgac cgttctcttt cagatagtta tccaaccatt cgatcgcgtt atgaccttcc
    19441 ttgataaagt tcccgccgac ccatgcgaca taaagggaat catcggtaac tttgatcatc
    19501 cggtcggaaa gaatgacggg gattccggca gcttttgctt cgcccaacac cgtctcccat
    19561 ccggtttcaa cgatcggagc taaaacgatg taatccactt cctgttgaat gaagtttcga
    19621 attgctttaa tctggttttc aggcttctgc atcgcgtcat caaaaatcag cttatagccg
    19681 ttttcttcgg taaatgttga tttaaaggac tcggtattgg cagtccgcca gtctgattcc
    19741 gcgcctactt gggaataacc gacaacaatc aaatcatcgg cagcgcttac ggcgacagca
    19801 aaaacgccgg acaacaataa aacaaaaaca agtaaactaa taaccttctt catcttccct
    19861 cctaaattga tttcggcttg aatagccggc tgaatattgt taaattcaat tgtatggata
    19921 atccgaaaaa gactctacgg aaaatcttat ggagaaagtt tagtttttta atcgaattat
    19981 ttcgaaaagt gggattttat ctttaaaaag ttaattttat ttaaagtcca tcaaatattt
    20041 aatattcccg agtaggtaat cgttcggcgg catttttaat gtcaaagatt tcctcgttta
    20101 cgtaaatctt tttctcaacc ggttcacccg cttccaattt acggataatc tcagcgaccc
    20161 gcggaccatg aagcgggttg cattcaatgg aagcattaat tcttccggcg acgatttctt
    20221 ccaaagctgc tttcaccgca tcaatagaaa tgataataat atctttcccg gggactaatc
    20281 cggcagcttc aatcgcatca atcgctccga aagccatatt atcattttga gcaatcaaaa
    20341 catcaatatc gtcatatttc tttaaaaagc cctccatcac ttcctgcccc atcgactgaa
    20401 caaaatcacc cgtttgtttt tccaataatc tgaaattagg atattcagct attttttttt
    20461 cgattccctc cgtcctgcct atttccgcgg tggagcctaa agttccctgc atttcgacga
    20521 tattaatgat ttctttatct ctccctattt cctttaggta attatgaagc cattcaacgg
    20581 aatcttcacc ctctttcaca aaattaccgc cgacccatga aacatacagg gaatcatctt
    20641 caacattgac cattctgtcc gaaagaatta agggaattcc ggcttccttt acttctttca
    20701 gaactgaatc ccaaccggat tcaacaatcg gagccaaaac gatataatca acttcttgtt
    20761 gaataaagtt tcttaatgcc ttaatttgat tttccggttt ttgttgagcg tcgataaata
    20821 tcaattcaaa cccgttttct tctgtaaaag tggatttaaa agattgggta ttggcaactc
    20881 gccaatctga ttccgctccg acctgtgcat atcccactac aatcaaatta ctttcggtcg
    20941 gagtgacaag tgaggagcgc tgtgcattcg gacggtaaaa acatccggct agacataaac
    21001 ttaccaaaaa aacggtataa gacaaaatct gtttcttaat catatctttt gctcttccga
    21061 tcgtatcaga tttaatggaa gttttatagt gacttctgtc cctaaatcat attcactttc
    21121 gatagataat ccgtattcgc ttccgtaata aagctgaatt ctttcatgga cattggataa
    21181 gccgaatcca attttgtgtt cctctaaaac ggattttcgt aatttttcca aattttcctg
    21241 tttcataccg atgccattat cctttacctg aaggaataat tcacattgag attgccaggc
    21301 tttgacggta attttcccca ttcccctttt atttttaatt ccatggtaca aagcgttttc
    21361 gacaagaggc tgtaaagtca gcttagggac gctgtaatct aaaagctctt ccggaattac
    21421 aatctcatat tccaaaatat cccgatagcg tactttttga atctcgagat aactgcaggc
    21481 atgaacttct tcttcccgaa gcgaaatgat atcttcgccc ttacttaagg tcgttcggaa
    21541 aaatacggat agggaagtaa tcatttcaat cactttttca tcctgatgat cttcggcaag
    21601 ataaatgatc gtatcaaaag tgttatacaa gaaatgcggg ttgatctgtg cctgtagaag
    21661 tttcaattcc gtttgccgaa gattttcctg ctcgtgcttg acatcattca aaaggatttc
    21721 gatttggcca accatactgt caaatgtatc attcaacatt tgaatttcat cgtcatatcc
    21781 tttttcggaa cgaacagtaa aatctccccg accgaccatc ttgaaatttt cagaaagcat
    21841 tcgaatcgga cgaacaatac tgtcggaaat acggaaagaa aagatccata acaagatgac
    21901 gataccaatg gaaacgatgc ccaaaatgac gatagtccgt gtgatttcct gattggtccg
    21961 ttcacgaata accgctaact cgttagtttc ataaaaaatg tattcttgaa ctttctcttg
    22021 aatcaactcc gtcaaaactc ggatatcata atccacactg gtcatatttt cttgataaga
    22081 atgtgtttca tggatcttct caattttggt ccttaaattc atcaggaaag cactgattgt
    22141 ttggagccgc ttgatattgt ccgcccggac tgttgttaat ttcaattttt cgaccacatt
    22201 cagcgcatta cttacatcgg aaagcggatc caatgtatca aaatcatcac ctccgccgat
    22261 tacatattgg tacatcttat aatccagcgt ctctttaaaa tcgaaattaa actcgctggc
    22321 ggtgacaagg ttttgcacga tcaaattata ttggttggta gaacgaatta atactgccaa
    22381 cataacaaca ataattaaag tcattgttag catgatgatc gtcatcagca tattcaactt
    22441 aaaacttaaa gaggaaaatc taattcgatt ggaaaagtat ttcctgttca attattctct
    22501 tctttcccgg tacggtattc cctcggagtt aatccggtct ctttcttgaa aagataactg
    22561 aaataatgcg aatccttata cccgaccgct tggctgattt ccgaactgcg taaagtcgtc
    22621 gatcggagaa gctctttcgc tttctcaata cgaacattgg ttaaatattc gataaaagtg
    22681 atcccttttt cctggctgaa aatcgaactg aaataagaag ggctgacatt tgtcacttgt
    22741 gcaaccagat ttaaggagat atcttccgaa ttgtaatttt cacggatata agtttcggcg
    22801 gcagataaaa gattggcata tttctgtttg ctattattgt ctcgaaaatc caaagccaca
    22861 tcaaatatct tctcaattaa ttcctttgta tatgatacgg tcgtgacctt ctccgtaatt
    22921 tgttcaatgt ccccaaagga atcaataatg acgtgttttc cgatgccgat ctcatccaga
    22981 aaagcggaaa caccgatcag gatattcgtc acgatatact tgcggaaagg cagagaatta
    23041 ccggaatcca aaccgatact ttcaatataa tcctcaacaa attgagaaac attctccttc
    23101 aacccgcttc gtaaaaatcg gtcgataacg gattggctta atttcgatgg atccaataac
    23161 ttcaaactga atccattttg gcttgttatt gcgggtttgg gatcgaacga ataatactcg
    23221 attttattat cttctacgat atagcgataa acaaaagccc ggttcgcttt ttgaaatgct
    23281 tgcgggatct gctgtaaacg ttccaccggc atcccgactc cgataaaata atccatgctt
    23341 tgatcggatt gaaaaagatt ttccaattcg acgatacacc gttttgttac agactcggct
    23401 gattcatgat ctgtgccttt tatcagcaaa gcccagcctt caagcccgcg gcgaaacaaa
    23461 atcatgtgtt tccgcattga gaaattattt tcgattttct cttccatttg gaccagttta
    23521 ccggaatatt tttcccatga ggattcatct ttttgagagc agataaacaa gatgatgtta
    23581 taacttttcg ccgctaaatc aatatccaat tcattcgcac gatttaatat ttccggaacc
    23641 gaagttgaat cggaaatgag ctgtgaaaaa aattgacgtc gctgataatt ttcattttct
    23701 tgcatttctt ttcgataacg aatttgcatt tctcgcagat ttcgttcttc ttcgatcgta
    23761 tcgcgaattt tcaatagggt ttccaaaact ttggcacttc cgattggttt caacagatag
    23821 tccgtcacac cgatgctgat agcttccttc gcatactgaa attcatcata accgcttagg
    23881 ataataattt ttgtccatgg taattcgttt cggattaatc ggctcaacgc caacccatcc
    23941 ataaacggca tttttatatc cgttatgaca atgtccggtt taatttgttg gatcatcgga
    24001 taagccaatt cgccgtcact ggcttctccg gagaattcga atccgttttc gacccaatta
    24061 attctgtcac gaattccttc acgaactatg atttcatcct caaccaagaa tacttttaac
    24121 ataatgccct attcattttg aaaatttaaa acgattatag ctaattttac cgataatttg
    24181 ctgtctgact tt
//
LOCUS       DAOSMM010000029        23834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2143_length_23834_cov_14.339249, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000029 DAOSMM010000000
VERSION     DAOSMM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 23834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23834
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2143_length_23834_cov_14.339249"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            506..1762
                     /gene="larA"
                     /locus_tag="PK898_05790"
     CDS             506..1762
                     /gene="larA"
                     /locus_tag="PK898_05790"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOU19582.1"
                     /translation="MDYQLKYGKNNLSICLDDNSDVTVVNPKDSSALKDPKAALIQEI
                     LSPIGTCSLKEMIPKGDRIGIILNDITRATPSEMILSAILTVLSEVPTDRITLFVGTG
                     THRKNTDSELREMFGSAITESFRIIQNDSADESTQVFLGKTSFGHEIWLNRELVNCGL
                     VVLTGFIEPHFFAGFSGGGKAIMPGMAGNRTIFANHGAEMIANPHSVWGITSGNPIYE
                     EILEIAEKIERSFLVNVAMNSKHEINGVFCGEIRHAHQAGCDFVKQNVMVPVPKQFDI
                     VIGTNSGYPLDLNLYQTVKGISAAARIVKNGGTILIASECSDGIPEEGMFKTLLKETN
                     SPQAFFDKLREPEFLVQDQWQVQILMQILQKAKVCLYSDRLSDEQIRACHLIPIHSIE
                     EFCRKEGKGKSICIMPEGPLTIPYLT"
     gene            1956..3062
                     /locus_tag="PK898_05795"
     CDS             1956..3062
                     /locus_tag="PK898_05795"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU19583.1"
                     /translation="MTLKVGMLTSGGDCQGLNSAMRGVGKRLFENDPQTEVYGFLDGY
                     AGLMQSNYRVMKPSDFSGILTLGGTILGTSRQVFKTMNDPLYPNRSDGPTKLEGMIRT
                     YKDLNLDGLVILGGNGTHKTANLLSENGLNVITLPKTIDNDLWGTDMTFGFQSAVNIA
                     TEVIDGIHSTASSHGRVFIIEVMGHNVGWMTLHAGIAGGADVILIPEIPYDPLAVAAA
                     VNKRAREHKRFSIIAVAEGAKTKEEAKMSKKEYAEKIGNLPFLSVAYRLASILDKLTV
                     QEVRCTVPGHFQRGGSPCAYDRVLTTALGVAAADWVIQKKFGVMVGMVNNEIVGIPLK
                     DVAGKVKSVPTNHPLIQSARNIGIYFGDEDLENG"
     gene            3055..4602
                     /locus_tag="PK898_05800"
     CDS             3055..4602
                     /locus_tag="PK898_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19584.1"
                     /translation="MAKPLIVAAIDVGSHDVIMKIAGLKSDEPPKIIDEVQRTIPMGA
                     DTYSTGKISQNLLNQTIEVLKTFNKKMEEYKVNTVQAAATSAFREAANQLFVVEQIRR
                     LTGIEVEVLSSGMDSYYHQIALSETIPDFTEMIQTNVLIVNIGSGSIQLTLFNDGKYI
                     NTQNFRLGPLRIRELLGDLERHSTDFGGLMTEYISGELNYYKTFGPKRATYQNLIVLG
                     GSSRYIKHVAGWTSKKQGQIKKEDFESFFEKLKSGNPNDLPRLSQVPTEQETLMLPTC
                     IVIDEVLQFTGIDQLYISDVDLTTGMLYKMANELQKYRFKRDPDINRIESAIYLANRY
                     RTDKKHSQHVSHLAVGLFDLTVKIHGLPKIARVYLEMAAILHNIGKYVSMENDGVRSY
                     NIIQSNELIGLNERELEIVALTACFHNGRVHPNDPMLQKLTSDEKLMTLKLISILALA
                     NSLDAGHQQKIEIIKTRVEDDRLEIFISSDKDFTLELWNFNKHTGLFQALFGLHPEIR
                     LLKSKKI"
     gene            4720..6903
                     /locus_tag="PK898_05805"
     CDS             4720..6903
                     /locus_tag="PK898_05805"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOU19585.1"
                     /translation="MIDFNKVDLPAIEEFPSRFINRELSWLEFNERVLEEARDSENPL
                     FERLNFLSITGSNLDEFFMIRVASLKDQYNAGYTLPDPAGLLPEKQLEMISDRTHEMV
                     RKQYSTLLRSIVPALEKNRIEIINPSELNPAQKDYVKRYFESTVFPVLTPLAVAAGSP
                     FPLIANKTINLIVRVEIPTAKNQKGQRMPFAVVQIPAILPRLLQLPSEEGDGQFILLE
                     DVVSEHINRLFSGVKIGTISCFRIMRNADLDIDDEDAADLLSEIETQVRQRRWGEVIR
                     LEIQSGYDEEILPILTENFEISDRDLYEIPGPLDLTFLSKLPSFFKDRQDLFYPPFTP
                     QPSPAFYGKDDYFSVIREKDVILNHPYEDFDTVIELIRRAAEDPNVLAIKQTLYRVGG
                     QSPIITSLADAAIRGKQVLVLVELRARFDEENNIQWAKKLEQAGCHVIYGLVGLKTHS
                     KITLIVRNEEDGIRRYVHLATGNYNETTAKIYTDLGLLTCSEPFGRDATDFFNMISGY
                     SMPLSWRKLIPAPKWLRIDTLNRIEQEIKNAREGKPAAIVAKINSLVDPEIIDALYRA
                     SQAGVRITLIVRGICCLRPGIKNLSENIHVRSIVGRYLEHSRIIYFYNDGQEDLFLSS
                     ADWMPRNLDRRIELQFPVEEKSARMRVFHVLYVQLMDTDRARIMEPDGTYHRVDRRRK
                     EAFDSQVRLCHDAIEAAASLNPEPRMMDRYIPSEVNATAVFKDDK"
     gene            7010..>7539
                     /locus_tag="PK898_05810"
     CDS             7010..>7539
                     /locus_tag="PK898_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU19586.1"
                     /translation="MKKKETDIKSRDIRIIRIYFLVLIGGMGAANPFLNLYMSRQHFS
                     GAEIGLVISIGSVIGLFAAPLWTRIHMESRYPIKMLSVSLIMSGIATVLFGYQTAFLG
                     MAVFYCLRTLFNAGHFTTADMLALRAIDGTRVGYGSIRLWGSLGWSVVVLLTGWMNGK
                     FSIRSGFFLYAAMNLIA"
     assembly_gap    7540..7639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7640..8029
                     /locus_tag="PK898_05815"
     CDS             <7640..8029
                     /locus_tag="PK898_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19587.1"
                     /translation="ISDKMIRRFGPIKVIRTSLFVYSAVRLVVLLFPSVTTVILMRAL
                     GGIAFSLLTIGQVVFMNMTLHSEKAGTALVIFTITIPTLINILLTPLFGSIYDNLGAG
                     WLYLISMSGYLLGALIYNRYYQKHPVF"
     gene            8190..9335
                     /locus_tag="PK898_05820"
     CDS             8190..9335
                     /locus_tag="PK898_05820"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19588.1"
                     /translation="MRHCRMKILFMSSVLIFIGWLISAEVSFATDNENCQSAYLQAGN
                     CVYKTFNNGRLRIRTSPDTSTNINIIANLDEGTLMTVLSGPICNSGYVFWKVRTANGI
                     VGYVAEGDNDQHWLTPIDPVNCNYLNQKTVPTELPSDSKGKYCPLRDCACSYLRVGDF
                     VKVSDSGPNAIRSDPDLHPADNIIYRAPSGSGMQIIEGPFCSWGWLVWKVRTDNGVEG
                     YTPESNGKQRWLIPDESRKNFPYDAWNRPYSPWFSPTSVPPSASYPMIQNAVGNCTLL
                     SSTPPYLATFSANEETDFSWTVRNDSGVTWTTDNYDIAYIGGTNLLKRKENIRRDFPY
                     DVPPGGTLSFIVDAVVPSFPGVYTMAYGVVQNYEIVCSVNVTIKVEY"
     gene            9531..9845
                     /gene="rplU"
                     /locus_tag="PK898_05825"
     CDS             9531..9845
                     /gene="rplU"
                     /locus_tag="PK898_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOU19589.1"
                     /translation="MKYAIIESGGKQYKAIEGAFVTVDRLEAEPGSELTLDQVLLVVN
                     DGSVSVGSPVVKGATVKAVVVGEEKGPKLTVFKYRPKKHYRAKTGHRQVYTRIKIEAI
                     EG"
     gene            9852..10121
                     /gene="rpmA"
                     /locus_tag="PK898_05830"
     CDS             9852..10121
                     /gene="rpmA"
                     /locus_tag="PK898_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOU19590.1"
                     /translation="MAHKKGGGSSRNGRDSNSQRLGVKKFGGELVLSGNILVRQHGTK
                     IKPGLNVGVGRDYTLFATTTGTVVYETLPSGSKRVNVAPLEISEE"
     gene            10183..10647
                     /gene="rpmE"
                     /locus_tag="PK898_05835"
     CDS             10183..10647
                     /gene="rpmE"
                     /locus_tag="PK898_05835"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOU19591.1"
                     /translation="MKKNIHPVYYTDAKVTCACGNTWTTGSIKKEIRTEVCSACHPFF
                     TGQQQRMLDMEGQVDRFYRRLQARQEFLEEKKSREEAKTSPDRPLEDLELSKRSLDAL
                     AKAGLYKVADVLDKLTSGGDEAMLTIEGFGQKSLIDLKKKLRALGYEVAGKE"
     gene            complement(10741..11364)
                     /locus_tag="PK898_05840"
     CDS             complement(10741..11364)
                     /locus_tag="PK898_05840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU19592.1"
                     /translation="MVNLNEIHDFFQNCFEQYGESPEGVGWNSDSAQEIRFNQLLKVI
                     QPADSFSLVEYGCGYGHLIDYMEKVGYHPSRYYGVDILESAIQAAKKRYGNNPAVSFH
                     RSLDEIPEADYLVASGVFNVKLDHDNVTWTRYIIESLDTFNLIAKRGFSVNFLTKYSD
                     YDRMVQRPDLYYADPCFLFDYCKANFSRNVALLHDYQIYDFTIIVRK"
     gene            complement(11339..12304)
                     /locus_tag="PK898_05845"
     CDS             complement(11339..12304)
                     /locus_tag="PK898_05845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU19593.1"
                     /translation="MTEEQISQSKQASVVIPVYNGAETIGPLCERLEKVLPALFSDYE
                     IVLVDDGSRDESWERIEYLCTQSGHIRGIRLMRNFGQHNALLCGIREARYPILITMDD
                     DLQHPPEQIPLLLAEFEKGYDVVYAIPKRLPHSWWRNLGSKLTKKVLSRVMHIQIQDI
                     GAFRIFKTELRKAFAKYSSPDVYIDPMLSWGTTKFSHVYVEEEKRPVGHSNYNFGSLV
                     RAALLILTGYSTLPLRFASTVGFVFILFGIGILIYVLIISLTMGSVPGFPFLASIISL
                     FSGVQLFSLGIMGEYISRMYEKSIDRPPYIVAEMKENNGQSQRDS"
     gene            complement(12301..14415)
                     /locus_tag="PK898_05850"
     CDS             complement(12301..14415)
                     /locus_tag="PK898_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19594.1"
                     /translation="MTKSGQTEKLLKKNKWSFDDRWIPLVLLFLSVLAFVPLIRSVGF
                     YWDDWPMLWFEITQGPQGFTQAFTSDRPFLGYLYQITGALIGTDPLNWQIVTVLFRWL
                     VSCAFWWCLRLLWPKEKDKTFWIAALLAVYPGFKQMPIAYVWGNAFIMLFSYVVSYGL
                     MLKAIASAENGNRKAYRLWTIAGVITYAFCTVCTEYYTGLDVVRGIIIWIFLLQYTEF
                     SDRKVSKKIRKTIWYWLPYLAVLILFMFWRIFIFQFHNYQPVLLSRFAANPIQTTGEL
                     FARVIEDAYTATWGAWTEYFSFPTRANLQTASDKMFWIAVVVGFLFIFAALILFRKKS
                     VQNKSDISDATEKKSWIRNAIILGSAAVVFPGIPYLVTSLSPSLDFPRDRWLTAYMFG
                     SCVFLVGLIDSFIRDGIKQKIIVSLFIAMAVGGNLLNTNTYRRDWEAQQDFINQLTTR
                     IPDLDTPAYFLTDFNPLTYETDNSLTGLFNLALEPENSSLTLPVSVGFYDVRFNSSAD
                     KFAAGEPIYQGFRSALYQGETKDIVVYFYSPPGCLRILDPEQHMNLPIFPDKFYEFIP
                     YSHPENILPEGTRSTFLQDEIFKKPVTKNWCYYFQEADLARQYSQWEKIAEIGDQVLN
                     RYQAGEASEYIPFIEAYAQLNRWGDTVPLIEKVHSMNKELDKPLCPILQKLFIEHYPQ
                     DQTEREEVASALYRIGCSAYSQ"
     gene            14785..15522
                     /locus_tag="PK898_05855"
     CDS             14785..15522
                     /locus_tag="PK898_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU19595.1"
                     /translation="MAKTNFSTYLSIKQQLLDMFKNEEYKNNKLPPESELADMLGISL
                     VTLRESLLMLALEGYITKRHGAGNYIHRSTLDFENRSIFFSECLKNAGYTPDIKVVSQ
                     EIHPATKEVSQALNISENDPVLENKIIHTADGSPAIYTVLTIPKSLLTNTSIETMDFS
                     YLHELIWKFCHKNLAHSLNEYVPMGLSPEVAQIFSLEPGTPIIACKQVFYDTFDIPVI
                     YSMSYFYPNLYRVRTLQNWALNVSSPQ"
     gene            complement(15611..17392)
                     /locus_tag="PK898_05860"
     CDS             complement(15611..17392)
                     /locus_tag="PK898_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOU19596.1"
                     /translation="MHIVIVKLKNKMHRDKNKKNNFFYILGGIGIIFMIWLFYDSFYK
                     EEIWYQNYSPMPKYPFLTILTAISPPIILFGMMILTKIRTSYASLISLLSAFLISVLI
                     WEMPPQTAISSVFYGAANAIFPILWTLLNAMWILNMLLKSGCFETLKESLKNTSDDRR
                     MQTILVGFGLTALLESMAAFGAPIAVITPMLIGLGFSPYLSATIALIADSAPSAWGTQ
                     SLPILMLSSVTNINIETLGKIVSHQAPLISALLPAALVFIMSGWKGLLGIFPIPFIIG
                     ATYWAVAILSARYLSVSAVGVLSAVSVIIVTLIINKIWKPKRSWIFPTEKESLSKKSA
                     SNNKASILYTDTIRSWSPYIFLILIIGITNIGGIKNFLNSFGFVEFPWPFLDNHVMKI
                     PPVVPVPTVYPAIYKQNLFNNGGTLMFISGILSTFTLKLSPRQAFHCFIKTWKDILES
                     GVTMICILGIAYLMNYSSMTYSIGLILTSVGKTIFPAISVLLGVLGCILTGSVAGSNA
                     LFGNLTVVVAQQLGLNPSFIAASLCSGGTLGKTLTPQNLIIAGAAIGEKNSGVEKKLI
                     YRVSVTTAFFIILLTALVLIQYGQSFS"
     gene            complement(17408..18745)
                     /locus_tag="PK898_05865"
     CDS             complement(17408..18745)
                     /locus_tag="PK898_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU19597.1"
                     /translation="MKCDLYIKDCMLLTKDYEIIDHQSIVINNSFIIEMGPTSTLDTK
                     YEPQQTITATDKLAMPGFIDGHTHTSQQLLRGRISDEYPVIYRRFNIPYESKLTEDDI
                     ELCTELSCLEMIKSGITAFADAGSMHIPAMINVVDRSGLRASITPAISDNDPTLPPNM
                     SDTKENALKKSENLFLEYNGKCDGRIHIWFQFRSITTCTSELIRELAKLAAYYHTGLH
                     THISEYPESILQSLNSYGMREIKYLESLDALSPNLLAAHCILLSEEDIHILKNHDVKI
                     VHCPRSNLGKGITKTPQLLGEGLSVGLGTDGTAHSGLNMFKEITAFKHSQIAAWGVPY
                     SDYDVMDSKTLLNMATLGSAKAMRIDDITGTLEINKKADLILIDMDQPHLMPTHSLLN
                     TLVEAGETADIKDVIVDGKILMQNREVKVLDEEHILSKGKQRLPKIANENGWN"
     gene            complement(18861..19790)
                     /locus_tag="PK898_05870"
     CDS             complement(18861..19790)
                     /locus_tag="PK898_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19598.1"
                     /translation="MESNYGITVQGLLAAAVRMATPLLLAGLGGLFSAKSGIVSMFLE
                     GFMLVGAFMGFLGSVLTGSAYLGILFAIAGGMLMGLIMAVLAVSGRANQTILGVGINI
                     FALGITSYFQSVLFGSKRPTNVSVFYDIPIPGLSKIPYIGAIFNNSLFVYAAFLLVPV
                     VWYVLYRTPAGLAIRAVGENPDSVATLGGNVIKIRYCCLLIAGALAGLGGASLTIGQT
                     GQFMENISSGRGYIALAAMIFGRFSPIGVLIGSLLFGFADGLQLRMQTAGTAIPYQLL
                     SMMPYVVTILALVVFVKKSMAPAAMGRPYTKQK"
     gene            complement(19798..20898)
                     /locus_tag="PK898_05875"
     CDS             complement(19798..20898)
                     /locus_tag="PK898_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19599.1"
                     /translation="MMKPLINKKNVTKHSHNKGRIQAISSFLAILMALLLSGLTISIT
                     GVNPFAAIKVLFSGAFGSSYAFSETLLRTTPILIASIGLAISFRCNLTSVGAEGQMII
                     GAIAATFTGLKISLASPVLEILVCIIAGFLGGALFGAIPGYLKAKLGTSEIIVTIMLN
                     YIAIDLLSYLLDGPMREGGSFYPQSALLDQSVWLPKIIAGTRLHAGFIIAIVFDIIYF
                     FLMFRTPLGFKIRAVGFNSKAAEYAGVNVGRSILTAMMLSGGLAGIAGAIEIFGVHHR
                     LLNGFTAGVGFDALAVALLGKLNPVGVFLASLFFAALKVGSNTMQHVMQVPTSIVYVI
                     QGLLILFVFTDTLIKDKLLYFEKRLKTKQSGE"
     gene            complement(20895..22457)
                     /locus_tag="PK898_05880"
     CDS             complement(20895..22457)
                     /locus_tag="PK898_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19600.1"
                     /translation="MENEETTSANQPLVEMRAITKTFQNVIANNCVDLSVNRGEIHAL
                     LGENGAGKSTLMNVLYGLYAPDSGEIYLNGEKIIMKSPADAIAKGIGMIHQEFMLIPT
                     LSVIENIILCMKSDNALKLDLQKAAKRLVDLSSRFGLDIDPWAKVSQLSVGEQQRVEI
                     LKALYRGTNILIMDEPTAVLTPKEVDDLFNVLKEIVKEGNSVIFISHKLGEVLNISDR
                     ITVLRNGKKITTVNSKDVSREQLAKMMVGREVFLQYDRPAVSVGKPVLKMSDVCCNND
                     RGLVALSNISLEVREGEIVGIAGVDGNGQMELAEVIHGLRKVTSGRIEINSVDTTNCS
                     SKFAIEVGLSHIPENRQIRGLVLDFSVAENIALINCEKPPFTKNGIYFDPQNAVRVAE
                     KLKNQYEIKCSNVNSPVKQLSGGNQQKVVLAREIYRQPKLLIAAQPTRGLDIGATEYV
                     QKLLIEKRSENMGILLISSDLDEILAISDRILVIYEGKIMGEAIPGKTSVVYIGLLMA
                     GSKVSEESRGKE"
     gene            complement(22536..23639)
                     /locus_tag="PK898_05885"
     CDS             complement(22536..23639)
                     /locus_tag="PK898_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOU19601.1"
                     /translation="MRFKKILSFAFILLIILIAFTACSKQTPTPVPTATSIPVESPTV
                     TAVADQTSEKDEKSSGTTKKVALLLTGTINDAGWSESSYKGLMKAKEKYGIETAYTEN
                     LALVDMESTARDYAAQGYDIIIFSSADFYQAAISVAPDYPNIRFVIVNGEGAQEPNLA
                     NFRPNTPECGFIAGSFAGLITKTNKVGMIGGKMLPPVVDAANGFTAGAKYANPNAEVV
                     TSYIDSWTDIAKGSEATLAMIESGVDVVVSNTGQAALGTIDAAKKKGIYAIGYIEDQH
                     DIAPGTVPFSAIQDIGDLVYASVTSALSDDFKPETVLVGAKDNVIRLSDFYTMGDQPV
                     PDEVIAKMKEIYQGIVDGSLKEQGILPKSGFEK"
BASE COUNT         7003 a         4871 c         5091 g         6769 t
ORIGIN      
        1 aatttcaaaa ttgataagaa agaggattat cgatgaaatc gcccaaaatc cttttcttaa
       61 aagcccgacg aatttatttt tgtcagcgcc catttctatc tccgttcttt aaatacaaaa
      121 gctttatcca gaaatttcaa agaactccat catcgttatc gggctaaatg atcgatttgt
      181 acttcataca aaggttatgt tgtatatact tattatagtg tgtggcgaaa aaaagaaaag
      241 tgtgaatttt atcttgtctc ttctttttgc cggttggaac agagaagaat tttgaatttc
      301 catggccccg atgttttcga aactaagacg gaaaggcaat gaggaccaac ggtaaaggta
      361 taatgaaatt ataaagaaat ggctatgttc tttatccgat tctaaatatc gaatgctgtt
      421 ttattaaagc ggtgaattgt tttttcgaaa aaaatttcag attattgttt tcgaagcggg
      481 tcctatcaaa taaaagaggc tctttatgga ttatcagttg aaatacggga aaaataattt
      541 atcaatttgt ctcgacgata attcggacgt tacggttgta aatccaaaag attcctccgc
      601 tttgaaggac ccgaaagcgg cattaattca ggaaatccta tccccgatcg ggacctgttc
      661 acttaaggaa atgataccga aaggcgaccg aatcggcatt atcttaaatg atattactcg
      721 cgcaactcct tccgaaatga ttttgagcgc gatcttaacc gtgttatcgg aagtcccgac
      781 cgatcgtatt actttgtttg tcggaacggg gacgcatcga aagaacacgg atagcgagct
      841 gcgtgaaatg tttggttcag caatcacaga gtcgtttcgc attatacaaa atgattccgc
      901 tgatgaatcc acccaagtat tccttgggaa aacttcgttt gggcatgaaa tttggttaaa
      961 tcgggagttg gtaaactgcg gattggtagt actgaccggc tttatcgaac cgcatttttt
     1021 tgctggattt tccggcggcg gaaaagcaat catgccgggc atggcgggga accgaacgat
     1081 tttcgcgaat cacggagcgg aaatgatcgc aaatccccat tccgtttggg gaatcacaag
     1141 cgggaatccg atttatgaag aaatactcga gatagccgag aaaattgaga gatcattttt
     1201 agtgaatgtc gcaatgaatt ccaaacatga aatcaatgga gttttttgcg gagaaattcg
     1261 tcatgcacat caggccgggt gtgattttgt caaacaaaat gtgatggtgc cggtccccaa
     1321 acaatttgat attgtaatcg gaacgaattc gggttacccg ctggatttaa atctttatca
     1381 gacggttaaa ggaatcagcg cagctgccag aattgtgaaa aacggcggaa ccattctgat
     1441 tgcttcggaa tgcagtgacg gaattcccga agaaggaatg ttcaagacgc ttctcaaaga
     1501 gaccaattca cctcaggctt ttttcgataa gctccgggaa ccggaattcc ttgttcaaga
     1561 ccaatggcag gtccagatcc tcatgcaaat tttacagaaa gcgaaagtct gtctttactc
     1621 cgacagatta tcggacgaac aaattcgggc ttgtcacttg atcccgattc attccatcga
     1681 ggaattctgc cgcaaggaag gaaaaggaaa atccatttgt attatgcctg aaggtccgtt
     1741 gacgattcct tatttgacat aaaaaaataa aataatcatc tgaagttgat tatgaaaagc
     1801 gaaaaagaaa acaaaaacga attaggagga acgatcaatc aacccgatcg ttttattaca
     1861 aagattcgga aatcctgagg ttcggcagaa tttaatatct gaatttataa tttatataga
     1921 gacgaattgt tcctttagat gaaaggggta aaaaaatgac attaaaagtc ggaatgttaa
     1981 ccagcggggg ggattgccaa gggcttaatt ctgccatgcg cggggtcgga aaaagattat
     2041 tcgaaaatga ccctcagacc gaggtttatg gctttttgga tgggtatgcc ggtttgatgc
     2101 agagtaatta tcgtgtgatg aaacccagtg atttttccgg tattctgact ttgggcggaa
     2161 cgattttggg gacatcccgt caggtattta agacgatgaa tgatcctttg taccccaacc
     2221 gcagtgacgg accgaccaag ttggagggaa tgatccgcac ctacaaagat ctaaatttgg
     2281 acggattggt gattctcggc ggaaacggta ctcataagac cgccaacctg ctttccgaaa
     2341 atggattaaa cgtcataaca ttgcccaaaa ccatcgataa tgatttatgg ggaaccgata
     2401 tgacctttgg tttccagagt gcggtgaata tcgcaacgga ggtgattgac gggattcact
     2461 ccactgcatc ctcccatggg cgggtattta ttattgaagt aatggggcat aatgtcggct
     2521 ggatgacttt gcatgccggg attgccggcg gtgccgatgt tatattgatt ccggaaatcc
     2581 cttatgatcc gctggctgta gccgcagcgg tgaacaagcg tgcccgtgag cataaacgct
     2641 tctcgattat tgcggtagcg gaaggtgcga aaacaaaaga agaagcaaaa atgagcaaaa
     2701 aggaatatgc ggaaaaaatc gggaatcttc ctttcttatc cgttgcttac cgattggctt
     2761 cgatcttgga taagttgacg gttcaggaag tccgctgtac ggttcccgga catttccaac
     2821 gcggtggttc tccttgtgct tacgatcggg tattgacgac cgctttgggt gtcgctgccg
     2881 ctgattgggt cattcagaaa aaattcggtg tcatggtcgg gatggtcaat aatgagatcg
     2941 tcggaattcc cctgaaggat gttgccggaa aagtgaagtc cgtaccaacg aatcatccct
     3001 taattcaatc ggcgcgaaac atcggtattt atttcggaga tgaagattta gaaaatggct
     3061 aaaccactga tcgttgcggc aattgatgtc ggttcccatg atgtgatcat gaaaattgcc
     3121 ggattgaaat ccgatgaacc ccctaagata atcgatgagg tacaacgtac cattccgatg
     3181 ggcgccgata cgtattcaac cggaaaaatc agtcaaaatt tattgaatca aacgatcgaa
     3241 gttctcaaga cattcaataa aaagatggag gaatacaaag tcaatacggt tcaggctgcc
     3301 gccaccagtg cctttcggga agccgctaat cagctttttg ttgttgaaca aatccgcaga
     3361 ttgaccggca ttgaggtgga agtcctttcg agcggaatgg attcatatta tcatcagatc
     3421 gccttatcgg aaacgattcc tgattttacg gaaatgatcc aaaccaatgt tttgatcgta
     3481 aacatcggat ccggcagtat tcagctgact ttatttaatg acgggaaata cattaatact
     3541 cagaattttc ggctgggacc gttacgtatt cgtgagctgc tgggagatct ggaacggcac
     3601 tcgacggatt tcggcggatt aatgaccgag tatatttcgg gagaactgaa ttactataaa
     3661 accttcggac cgaagcgggc aacttatcag aatctgatcg ttttaggcgg atcgtcacgt
     3721 tacatcaagc atgtcgccgg ttggacgagc aaaaagcaag gtcaaattaa gaaggaagat
     3781 tttgaaagct tttttgaaaa attaaaaagt ggtaatccga atgatctccc gcgactttct
     3841 caggtaccta ccgaacaaga gacattgatg ctgccgactt gtatcgttat tgatgaagta
     3901 ttgcagttca ccggaatcga tcagctgtat atttcggatg tcgatctgac aaccggaatg
     3961 ctttacaaaa tggcgaacga actgcaaaaa tatcggttta aacgagatcc ggatatcaac
     4021 cggatagagt cggcaattta tttggcgaat cgttatcgca cggataaaaa acattcgcaa
     4081 catgtaagtc atttggcggt cggtctgttt gatttgacgg tcaagattca cggattgcca
     4141 aaaattgcgc gagtatatct tgaaatggcc gctatattgc ataatatcgg caagtatgtc
     4201 agcatggaaa atgacggtgt gcgaagttac aatattatcc aatccaatga attgatcggt
     4261 ctgaatgaaa gagaactcga aattgttgcg ctaaccgctt gctttcataa cggaagggtt
     4321 catccgaatg atcccatgct gcaaaaactg acttcggacg aaaaattaat gaccctcaaa
     4381 ctgatttcca ttttagcttt ggcaaactct ttggatgcgg gtcatcaaca gaaaattgaa
     4441 attataaaaa cgcgggtgga agacgatcgc ttagaaatat ttatcagttc tgataaggat
     4501 tttactttgg aattatggaa tttcaataag cataccggct tattccaggc attattcgga
     4561 ttgcaccccg aaattagatt actcaaatca aagaaaatat aattttactt ttttcagtcg
     4621 actttcgaat ttgaccggct tcatcaaaaa aggaacagaa gacagatgta tggagatttt
     4681 tttgataatg atcaatcatt cctatcgcgg atagatgatt tgattgattt taataaggtc
     4741 gatttgcccg ccatcgaaga gtttccgagc cgttttatca accgggaatt gagctggctg
     4801 gaatttaatg aacgggtgct tgaagaagcg agagatagcg aaaatccgct gtttgaacgt
     4861 ttgaattttc tttccatcac cggttccaac ctggacgaat tttttatgat ccgagtcgct
     4921 tcacttaagg atcagtacaa tgccggctac accttaccgg atccggccgg actattgccg
     4981 gagaagcagc tggaaatgat ctcggatcgg actcatgaaa tggtcagaaa acagtattcc
     5041 actcttctgc gatcgatcgt ccccgcattg gaaaagaacc gaatcgaaat aatcaatccg
     5101 tccgaattga atccggcaca aaaagattat gtcaagcggt atttcgaatc aaccgttttc
     5161 ccggtgctga cgccgttggc ggttgctgcc ggctcgcctt tcccattaat tgccaataaa
     5221 accattaatt tgattgttcg ggtagaaatc ccgaccgcga agaatcaaaa aggtcagagg
     5281 atgccatttg ccgtcgtaca aattcccgca atccttcccc gtcttttaca gctgccttcc
     5341 gaagaagggg acgggcaatt catcttgttg gaagatgtcg tatccgaaca tatcaatcga
     5401 ttattcagcg gagtaaaaat cggaacgatt tcctgcttcc gaattatgcg aaatgcggat
     5461 ttggacattg acgatgaaga cgcagctgat ttactgagtg agatcgagac gcaagttcgg
     5521 caacgacggt ggggtgaagt cattcgtctc gaaattcaaa gcggatatga cgaagagatt
     5581 ctgcctattc ttacggaaaa ttttgaaatc agcgaccggg atctgtatga aatcccggga
     5641 ccgttggact tgactttctt atcgaagctg ccttcttttt tcaaagatcg tcaggatctt
     5701 ttttaccctc cctttactcc tcagccctcg ccggcttttt acggaaagga tgattatttt
     5761 tcggttatcc gggagaaaga tgtgatcttg aatcatcctt atgaagattt tgataccgta
     5821 atcgaattga ttcggcgagc ggcagaagat ccgaacgtgc ttgcaatcaa gcaaaccctt
     5881 tatcgggtgg gcggccaatc accgattatc acttctttag ccgatgcggc catccgcgga
     5941 aaacaggtct tggtattggt tgagctgcgg gctcggttcg atgaagagaa taatattcaa
     6001 tgggcgaaga agttggaaca agccggttgc catgtgatct acggattggt cggcttgaag
     6061 acgcacagca aaattacgtt aatcgtacgg aatgaagaag acggaattcg acgctatgtg
     6121 caccttgcga ctggcaacta taatgaaaca actgccaaaa tttataccga tttgggactt
     6181 ctgacttgtt ccgaaccgtt cggtcgcgat gccacggatt ttttcaatat gatttccggt
     6241 tattcgatgc ccctatcctg gcggaaattg attccggcgc cgaaatggct gaggatcgat
     6301 actctgaatc ggattgagca ggaaataaaa aatgctcggg aaggaaaacc ggctgccata
     6361 gtggcaaaga ttaattcgct ggtagatccg gaaattatcg atgctttgta tcgcgcttcg
     6421 caagcggggg tgagaattac cttaattgtt cgcggtattt gctgccttcg cccgggcatt
     6481 aagaatttat cggaaaatat tcacgtccgg agcattgtcg gacgatatct cgaacattca
     6541 cggattattt atttttataa tgacggtcag gaagacctgt ttctttcgag cgctgattgg
     6601 atgccgcgaa atttagatcg ccgaatcgaa ttacaattcc cggtcgaaga aaaaagtgca
     6661 cggatgcggg tatttcatgt tttatatgtt cagctgatgg atacagatcg ggccaggata
     6721 atggagccgg acgggacgta tcatcgggtg gatcgtcgtc gtaaagaagc gttcgacagt
     6781 caggttcgat tatgccatga tgccatcgaa gctgcggctt cattgaatcc cgagccgagg
     6841 atgatggatc gttatattcc cagtgaggta aacgcgaccg cggttttcaa agacgacaaa
     6901 taaattatag aaaacaacga tgatttattg acacgatttc cttgagatga gatcattttt
     6961 tattcggatt cccttaaaaa taatggatta tctgatacat taaagcaata tgaagaagaa
     7021 agaaacggat attaaaagcc gtgatatcag gattatccgc atctatttct tggtcttgat
     7081 cggcggaatg ggtgcagcta acccgtttct taatctatat atgtcccgtc aacatttttc
     7141 aggtgctgaa atcggactgg ttatttctat cggttcggtc atcgggttat ttgccgctcc
     7201 gttatggacg agaattcata tggagagccg ctatccgatt aaaatgcttt ccgtttcact
     7261 gattatgagc ggaatcgcga cggtactatt cggttatcag acagcatttt tggggatggc
     7321 tgttttttat tgtctgcgta cgttgtttaa tgccggacat tttaccaccg cagacatgct
     7381 ggctttacgg gcgatcgacg gcactcgagt cggctacggg agcattcgtt tatggggttc
     7441 attgggatgg tcggtcgtgg tgctgcttac cggttggatg aacggcaaat tttcgattcg
     7501 atccggtttt tttctgtatg cggcaatgaa tttgatcgcn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnna tcagcgataa aatgatccgt cgattcggac cgataaaagt
     7681 aatccgtact tcattatttg tttacagtgc ggtgcggctg gtggtgttgt tattccccag
     7741 cgttacaacc gttattttaa tgcgtgcgtt gggtggaatc gctttcagcc ttttaacgat
     7801 cgggcaagtt gtatttatga atatgacttt acattcggag aaggctggaa cggcattggt
     7861 gatttttaca attaccattc cgacactcat caatattctt ttgacacctt tattcggctc
     7921 gatctatgat aatttgggag ccggttggtt atatcttatc agtatgtcag ggtatttgct
     7981 cggtgcgctt atttacaatc gttattatca aaaacatccg gtattttaaa attaaaattc
     8041 gttgaaataa ccgaataata aagactttat ctgacgctcg atgataaatt tgcctgtcaa
     8101 agtcaattca cgttgcgaaa ttgcaaccat tctgccggta aaaacgtctt tgaaatgaag
     8161 aataaatcga tcagagagcg gaggtttcta tgcgacattg tcgaatgaaa atactgttta
     8221 tgtcatccgt tttgatattc atcggatggt taatttcggc ggaagtatct tttgcaacgg
     8281 ataacgaaaa ttgtcaaagc gcatatttac aagcgggaaa ttgcgtttat aaaaccttca
     8341 ataacggtcg cctccgcatc agaacttcac ccgatacttc tacaaatata aatattattg
     8401 ccaatttgga tgaaggaaca ttaatgactg ttctttccgg accgatctgt aattcggggt
     8461 acgttttttg gaaagtgaga acggcgaacg ggattgtcgg atacgtggcg gaaggtgata
     8521 acgatcaaca ttggcttacg ccgatcgatc cggtgaattg taattacctg aatcaaaaaa
     8581 ccgttcccac ggaacttccc tccgattcga aaggaaaata ttgtccgctg agagattgcg
     8641 cctgttctta tctcagggtc ggcgattttg ttaaagtttc ggacagcggt cccaatgcaa
     8701 tccgaagtga tcccgatttg catccggccg ataacattat ttatcgcgct ccttccggaa
     8761 gcggcatgca aattatcgaa ggccctttct gcagttgggg ctggttggtg tggaaagttc
     8821 ggacggacaa cggcgtagaa ggttatactc cggaaagtaa cgggaaacag cggtggctga
     8881 ttccggatga aagcagaaaa aactttccat atgacgcttg gaatcggcca tattctcctt
     8941 ggttttctcc gacttccgtg ccgccttctg cctcgtatcc gatgattcaa aacgcagtcg
     9001 gaaactgtac gcttttatct tcaacgccgc catatctggc gacgttttcc gccaatgagg
     9061 aaacggattt ttcatggact gttcgtaacg attcgggtgt cacctggact acagataatt
     9121 atgacatcgc ctatataggc gggacaaatc tattaaaacg aaaagaaaac attcgccgag
     9181 atttcccgta tgatgttcct ccaggcggta cactctcttt tatcgtcgat gccgttgttc
     9241 cgtcgttccc cggcgtttat acgatggcct acggtgttgt tcaaaactat gaaattgttt
     9301 gttcggtgaa tgtgactatc aaggtcgaat attaattcgt gtagatgatc gaaaattggt
     9361 cgaatatcgc ataatatccg cgtacaaaat aattgatttt cgcagaacat aaggtataat
     9421 tgttgaggct tgatccggga atcaaacgga caagtttaat ttgccaactt ccccttacaa
     9481 cagcggaatg acgcgggtga taaggtgaag tgaagattgg agaatcggga atgaaatacg
     9541 ctattataga atctggcggg aaacagtaca aagcaatcga aggggctttt gtcactgttg
     9601 atcggctgga agctgaaccg ggatcggaac tgacgcttga tcaggttttg ttggttgtca
     9661 atgacggttc ggtttcggtg ggcagccctg tcgtaaaagg tgcgacagta aaagctgtgg
     9721 tggtaggcga agagaaaggt ccgaaattga ccgttttcaa atatcgtcca aagaaacatt
     9781 atcgagcaaa gaccggacat cgtcaggttt atactcggat caagattgaa gcgattgagg
     9841 ggtagtaaat tatggcacac aagaagggtg gcggttcaag ccgcaatggt cgtgatagta
     9901 attcgcaacg tcttggcgta aagaaattcg gtggtgaact ggttttgtcg ggcaatattc
     9961 tggtccgcca gcacggaaca aaaatcaaac ccggcttaaa tgtcggtgtc ggtcgagact
    10021 atacgctctt cgcaaccact accggaacgg ttgtctatga gacgcttccg agcgggagta
    10081 aaagagtcaa cgttgcgccg ctggaaataa gcgaagaata aaattgtcac tgaatcgtca
    10141 ccgaagcgga attaggcgac gaattgtgaa aggaaagaaa aaatgaaaaa gaatattcat
    10201 cccgtatatt atacggatgc aaaagtaacc tgcgcatgtg ggaatacctg gactaccggt
    10261 tcgattaaaa aagaaattcg tacggaagta tgttccgcat gccatccttt ctttaccggc
    10321 caacaacagc gtatgttgga tatggaaggg caggtcgatc gattctacag acggcttcaa
    10381 gctcgacaag aatttttgga ggagaaaaag tcccgtgaag aggcaaaaac ctccccggat
    10441 cgtcctttgg aagatcttga actcagcaaa cggtcattgg acgctttggc taaagctggt
    10501 ttgtataaag tggccgatgt actggataaa ctgacgagcg gcggcgatga agcgatgcta
    10561 acaatcgaag gtttcggtca gaaatcattg attgacctca agaagaagct gcgcgctttg
    10621 ggctatgaag tagccggaaa agaataagag ttttcttcgt actaaaaaaa atggctgcat
    10681 ttttgcagcc atttttttat cctaaaatta tttttctctc tcagtcagtt tttccggcaa
    10741 ttactttcgg acaatgattg tgaaatcata aatttgataa tcatggagca aggcaacatt
    10801 tctggaaaaa tttgctttgc agtaatcgaa caaaaaacag ggatcggcat aatacagatc
    10861 ggggcgttgg accatccgat cataatccga gtatttcgtc aggaagttta ccgaaaatcc
    10921 ccgttttgca atcagattga aggtatccaa cgattcaatg atatagcgtg tccaagttac
    10981 attatcatgg tccaatttga cattaaacac tccggacgcg accagataat cagcttccgg
    11041 aatctcatca agcgatcgat gaaaactgac agccggatta ttcccgtatc gtttcttcgc
    11101 ggcttgaatg gcgctttcca agatatcgac accataataa cgggaagggt gatatccgac
    11161 tttttccata taatcgatca aatgtccgta accgcaaccg tattcgacca atgaaaatga
    11221 atccgcgggc tgaatcactt tcaacaattg attaaaacgt atttcttgtg cactatccga
    11281 attccaaccg acgccttccg gcgattcgcc gtattgttca aaacaatttt ggaagaagtc
    11341 atgaatctcg ttgagattga ccattgttct ccttcatttc ggcgacgata taagggggcc
    11401 gatcgatcga tttttcgtac atacgggaaa tatattctcc catgatcccc agagaaaaaa
    11461 gttgaacccc tgaaaataag gagatgatcg aggccaggaa cgggaatccc gggacggaac
    11521 ccattgtcaa tgagatgatc agaacataga tcagaatgcc gatcccaaac agaataaaaa
    11581 cgaatccgac agtgcttgcg aaacggagcg gcaatgtgct gtagccggta agaatcagta
    11641 aagcagctct gaccaaagat ccgaaattgt aattggaatg cccgacggga cgtttttctt
    11701 cctcaacata gacatggctg aattttgtcg ttccccatga cagcatcggg tcgatataga
    11761 catccgggct gctgtatttt gcgaatgctt tgcgtaattc ggttttgaaa atacggaaag
    11821 cgccgatatc ctgaatttga atgtgcatta ctctgcttaa aacttttttt gtcagttttg
    11881 agccgagatt tcgccaccag gaatggggaa gtcttttggg aatggcataa accacgtcgt
    11941 aacctttttc gaattctgcc agcaacaagg ggatttgctc ggggggatgt tgtaaatcat
    12001 catccatggt gatcaaaata gggtatcgtg cttctcggat gccgcaaaga agggcgttgt
    12061 gctgtccgaa attgcgcatc agtcgaattc cgcgaatatg accgctttgt gtgcagagat
    12121 attcgattcg ctcccaactt tcatcccgac tgccgtcatc aaccaggaca atttcataat
    12181 ccgaaaatag tgccggcaac actttttcca accgttcgca gagtggtccg attgtttcgg
    12241 ctccgttata gaccggtatc acaaccgatg cttgttttga ttgactgatc tgttcctctg
    12301 tcattgagaa tatgcgctgc aaccgatccg gtacaatgcc gaagcgactt cttccctttc
    12361 tgtttggtct tgtgggtaat gttcgataaa gagtttttgt aaaatcgggc aaagcggttt
    12421 gtccagctct ttattcatgg aatgcacttt ctcgattaat ggcactgtat ctccccaacg
    12481 attcagttgc gcataggctt caataaacgg aatgtattcc gaagcctctc ccgcttgata
    12541 ccgattcaga acctgatccc cgatttcggc aattttttcc cactgcgaat attgtctggc
    12601 aagatccgcc tcttgaaaat aataacacca attttttgtc acaggttttt taaagatttc
    12661 atcctgtaag aaagtcgaac gtgtcccttc cgggagaata ttttccggat gagagtaggg
    12721 gatgaattca taaaatttat cgggaaaaat cggtaaattc atatgctgtt cgggatctaa
    12781 aatacggagg cacccgggag gcgagtaaaa ataaacaacg atatcttttg tttctccttg
    12841 gtataaggcg cttcggaagc cttgataaat cggttctccc gcggcaaact tatccgcaga
    12901 tgaattaaag cgcacatcgt aaaaaccgac actgacaggc agggttaaag agctgttttc
    12961 gggctccaat gccagattaa acaatccggt taatgaatta tctgtttcat aagtgagcgg
    13021 attaaaatcc gtcagaaaat aagccggcgt atcaagatcc gggatgcggg ttgtcaattg
    13081 gttaataaaa tcctgttgag cctcccaatc ccttcgataa gtattggtat tgaggagatt
    13141 tccgccgaca gccatggcaa tgaaaaggct gacaataatt ttttgtttta ttccgtctcg
    13201 aataaaactg tcaatcaaac cgactaaaaa gacgcagctt ccgaacatat aagccgtcaa
    13261 ccaacgatcg cgcgggaaat ccaacgaagg cgaaagtgag gtgactaaat agggaatgcc
    13321 gggaaatacg acagcagccg atcctaaaat gatcgcgttt cgaatccaac tttttttctc
    13381 agtggcatcg gatatatccg atttgttttg gactgatttt ttccggaaaa ggattaaagc
    13441 ggcaaaaata aacagaaatc cgacgacgac cgctatccaa aacattttat ccgaagcggt
    13501 ttgaagattc gcacgggtcg ggaacgagaa atattctgtc catgcacccc aagtagccgt
    13561 atacgcatct tcgatgactc tggcaaataa ttcgccggtg gtttggatcg gattggctgc
    13621 gaagcgggat aaaaggaccg gctgataatt gtgaaactga aatataaata tccgccaaaa
    13681 cataaacaga atcagtacgg cgagataggg aagccaatac caaattgttt tccgaatctt
    13741 ttttgaaact ttccggtcgg agaattcggt atattgaagg agaaatatcc aaataattat
    13801 gccgcgaaca acatccaacc ccgtataata ttccgtgcag acggtgcaga aagcatatgt
    13861 tatcacaccc gcgattgtcc ataaccggta tgctttgcgg ttaccgtttt ctgcggatgc
    13921 gattgcttta agcatcaatc cgtatgaaac aacgtacgag aacaacatga taaaagcatt
    13981 tccccagaca taagcaatgg gcatttgctt aaatcccgga taaacggcga gcaacgctgc
    14041 tatccaaaaa gttttatctt tttccttcgg ccaaagtaat ctcagacacc accaaaatgc
    14101 gcaggaaacc agccagcgaa ataaaacagt aacaatctgc caatttaacg gatcggtgcc
    14161 gataagtgca ccggttattt gatagaggta gccgagaaac ggacgatccg aggtgaaagc
    14221 ttgggtaaag ccctgaggac cttgggttat ttcaaaccat aacatcggcc aatcgtccca
    14281 ataaaagccg actgagcgga ttaagggcac aaaagcgaga acggaaagaa acagtaagac
    14341 cagcgggatc caacgatcat caaatgacca tttatttttt ttaagcaatt tctctgtttg
    14401 acctgatttc gtcatatttc gtattgttcc attttactcc catgatttta ccattgttct
    14461 acacgtgctc atttcgaagg gaaatagagc aatactgaaa aataatatcc gaaattaaac
    14521 tatacatcta aaagaaacaa agttatttta tattgcacaa aattgatccc tttgaaattg
    14581 catttttgat ggttgataaa aaataagaca gctttgagag caggatcccc gacgatcaac
    14641 aagtatgaat aatcatcgta taatagagat ataatgatta taagacattc agaaataaat
    14701 aaaacgatta ataactctca ggatgatctt ttataaagat catcctgaga tggtattttt
    14761 catggcatgg agaaaaaaat aaaaatggca aaaacaaact ttagtaccta tttatccatt
    14821 aaacagcaat tattagatat gtttaagaac gaagaatata aaaataataa actacctccc
    14881 gaatcggaac tggctgatat gttgggaata agtttagtta cattaagaga atcgttactg
    14941 atgcttgcgc ttgaaggata tataaccaag cgacatgggg cgggaaatta tatccatcga
    15001 agcactttag attttgaaaa ccgcagcatc tttttttctg aatgtttgaa aaatgccgga
    15061 tatacacccg atataaaagt tgtcagccag gaaattcatc ctgcaacaaa agaagtcagt
    15121 caggcattaa atatttctga aaatgatccg gttcttgaga ataaaattat tcataccgcc
    15181 gatgggtctc ctgcgatata tactgttttg acaattccaa aatcccttct gacaaataca
    15241 tccattgaga caatggattt tagttacttg catgaattaa tttggaaatt ttgccataaa
    15301 aatcttgctc attctttgaa tgaatatgta ccaatgggtc tgtctcccga ggttgctcaa
    15361 atattttcgt tggaacccgg tactccgatt attgcctgca aacaagtttt ctacgacact
    15421 tttgacatcc cggttattta cagtatgagc tatttttacc ccaacctata tcgagtaaga
    15481 actcttcaaa attgggcttt gaatgtctca tctccgcaat gatttgttcc gaaagaaatt
    15541 gaagatttcg actcgaactg cgtatatata acaaattatt cctgttgaat gcatcggttt
    15601 cagataggaa ttaactgaaa gactgtccat attggattaa caccaaagct gtcagcaaga
    15661 ttataaaaaa agcggttgtc acagaaaccc gataaataag tttcttctcg actccgctgt
    15721 ttttttctcc aattgcagct ccggcgataa taaggttttg tggcgtgaga gtctttccca
    15781 aagtgccgcc tgagcagaga gacgccgcga taaaggaagg atttaatccc agttgctgag
    15841 caacgacaac cgtaagattt ccgaataaag cgttcgatcc ggctacgctc ccggtcaaaa
    15901 tacaaccgag aacccctaaa agaactgaga ttgccggaaa tattgttttt cctactgaag
    15961 ttagtataag accgatcgaa taagtcatcg aagaatagtt catcaaatag gctatcccga
    16021 gaatacaaat catggtaacc ccagattcca aaatatcttt ccatgttttt ataaaacaat
    16081 gaaaggcttg ccggggagac agtttcaaag tgaatgtgct caaaattccg gagatgaaca
    16141 ttaatgttcc gccattgttg aaaagattct gtttataaat tgccggataa actgtaggaa
    16201 cgggaactac gggtggtatt ttcattacgt gattgtctaa aaacggccac ggaaattcta
    16261 caaatccaaa agagttaaga aaattcttta taccgccgat atttgttatt ccaataatca
    16321 ggataagaaa aatataaggc gaccaagaac gtattgtgtc ggtatataaa attgaggctt
    16381 tattatttga agcgctcttt tttgataaag attctttttc tgtggggaat atccaacttc
    16441 gtttcggctt ccaaatctta ttgattatca gcgtaacaat aattaccgaa accgctgaaa
    16501 gaaccccgac ggcagacaca gaaaggtatc gggctgataa tatcgcgaca gcccagtaag
    16561 tagctccgat aatgaatggt atcgggaata taccgagcag ccctttccat cctgacataa
    16621 tgaaaacaag agcggctggt aataatgctg aaattaatgg cgcttgatga ctgacgattt
    16681 ttccgagagt ttcaatatta atattcgtaa ccgaacttaa cataaggatc ggaaggcttt
    16741 gagttcccca tgcgctcgga gcagaatccg ctatgagcgc gattgttgcg gataaataag
    16801 gtgaaaatcc taagccgatc agcatgggtg taataacggc tatcggtgcg ccgaatgcag
    16861 ccatactttc aagtaatgca gtcaaaccaa atcccactaa tatcgtttgc atccgacggt
    16921 catcacttgt atttttgagt gattctttca atgtttcaaa gcatccgctc ttaagtagca
    16981 tattcagaat ccacattgca tttaataatg tccataaaat agggaatatc gcatttgctg
    17041 ccccataaaa cacactgctg attgctgttt gagggggcat ttcccaaatt aatacggata
    17101 ttaagaatgc tgataaaaga ctgatcaggg aagcataact tgtgcggatc tttgttaaaa
    17161 tcatcattcc gaataaaatg atcggaggag aaatggcggt tagaatagtt aagaacggat
    17221 atttgggcat cggtgaataa ttttgatacc aaatttcttc tttatagaag gaatcataaa
    17281 ataaccaaat cataaagata ataccgattc caccgagaat ataaaaaaaa ttattttttt
    17341 tatttttatc ccgatgcatt ttatttttca atttcacgat tactatgtgc attttttccg
    17401 atttttatta attccacccg ttttcatttg cgatttttgg cagacgttgt ttccctttgg
    17461 acagaatatg ttcttcatcc aagactttga cttcacggtt ttgcatcaga atctttccgt
    17521 ctacaattac atctttaata tcggctgttt cgcccgcttc cactaaggtg ttcaacagac
    17581 tatgtgtcgg cattaaatga ggctggtcca tatcgatcaa tattaaatca gccttcttgt
    17641 ttatctcaag agttccggta atatcatcaa ttcgcatcgc ttttgcactt cctaaagtcg
    17701 ccatattaag aagtgttttg gaatccatca catcataatc ggagtatggc acaccccaag
    17761 cagcgatttg tgaatgttta aaggccgtta tttctttaaa catattcaaa ccggaatgtg
    17821 ctgtaccatc cgtcccgagc ccaactgata acccttcgcc taataattgc ggagttttcg
    17881 ttataccttt ccccaaatta cttctcggac aatggacaat cttgacatca tggtttttca
    17941 atatatgaat atcttcctcg gataataaga tgcaatgtgc agccaataaa ttcggactaa
    18001 gcgcatcgag actttccaga tatttgattt cccgcattcc gtaactgttt aaagattgta
    18061 gaatcgattc gggatattca ctgatatggg tatgtaatcc tgtatgataa tatgccgcta
    18121 atttagctaa ttctcgaatt aattcacttg tacaagttgt gatcgatcga aactggaacc
    18181 aaatatgaat tctgccatca cactttccgt tatattccaa aaacaagttt tcgctttttt
    18241 tcaatgcatt ttcttttgta tcagacatat tgggcggcag tgttggatcg ttatcagaaa
    18301 tggcaggcgt gatggaagct cgtaaccccg atcgatcgac aacgttgatc attgcgggaa
    18361 tatgcatgct tcccgcatcc gcaaatgcag taatgccgga tttaatcatt tccaaacaac
    18421 tcaattccgt acataattca atatcatctt cggtcagctt actttcatat ggaatattaa
    18481 atctgcgata aattaccgga tattcatcag aaattcgacc tcttagtaat tgctgggaag
    18541 tatgtgtatg accgtcaata aatccgggca ttgctaattt atcagtcgct gttatggttt
    18601 gttgcggctc gtattttgta tccaatgtgg atgtgggtcc catttcgata atgaatgaat
    18661 tattaataac gatggattga tgatctatga tctcgtaatc ttttgtcagc aacatacaat
    18721 ctttgatata taaatcacac ttcataaatt tttccttccg ggttaccgca atacgggttt
    18781 tgtttattcg aatccgatct tactttgaca tcacgataaa agaaaaaaga taaatatttt
    18841 attcaaaata acaatcaaca ttatttttgt ttggtataag gacgtcccat tgccgctggt
    18901 gccatcgatt tcttgacaaa taccacaagc gctaagatcg ttacgacata aggcatcata
    18961 gaaagcaatt gataaggaat agctgtcccg gcagtttgca ttcttaattg caaaccgtcg
    19021 gcaaaaccga ataaaagcga accgattaac actccgatgg gagaaaatct gccgaagatc
    19081 atagctgcca aagcaatata acctcttccc gatgaaatat tttccataaa ttgacctgtc
    19141 tgcccgattg tcaatgaggc accgcctaac ccggccaggg ctcctgcgat taaaagacag
    19201 caatatcgga tttttattac atttccgccc aatgtggcga ccgaatcggg attttcgcct
    19261 acagctctaa ttgcgagacc tgccggggta cgatataaaa cataccaaac aaccggaacg
    19321 agcaaaaagg ctgcgtaaac aaataaagag ttattgaaaa tcgcaccgat gtaggggatt
    19381 tttgacaaac caggaatcgg aatatcataa aaaaccgaaa cgtttgttgg gcgtttggac
    19441 ccgaataaga ccgattggaa ataactggtg atgcccaaag cgaaaatgtt aattcccact
    19501 ccgagaatcg tttgattggc tctgccggaa acagctaaga cagccattat caaacccatc
    19561 agcattcctc cggcaattgc gaataaaatt ccgaggtaag cgcttcctgt caaaacggag
    19621 cctaagaatc ccataaacgc tccaaccagc ataaatcctt caaggaacat actcacaata
    19681 ccggattttg ctgaaaacaa acctcccaat ccggctaaaa gcaaaggcgt agccattcga
    19741 actgcggcag ctaaaagacc ttgaacggta attccataat ttgattccat gatttgatta
    19801 ctccccggat tgttttgttt ttaatctttt ttcgaaataa agcagtttat ctttgattaa
    19861 ggtatcggtg aaaacaaaca gtattaaaag accttgtatg acataaacga tcgaagtcgg
    19921 tacctgcatc acatgttgca ttgtattgga cccaactttt agcgcagcaa aaaagagcga
    19981 ggcaaggaaa acaccaacgg ggtttaattt tcctaagaga gctactgcca atgcatcaaa
    20041 tccgacaccg gcagtaaaac cattcagcaa tcgatgatgg accccgaata tttctatcgc
    20101 acctgcgatt cccgcaagac cccctgaaag catcatggcg gttaatatac tccttcctac
    20161 attaactccc gcatattctg ccgctttaga attgaaacca accgcacgta ttttaaaacc
    20221 gagtggagta cgaaacatca agaaaaagta gataatatcg aaaactattg caataataaa
    20281 accggcgtga agtcttgtcc ccgcaattat ttttggaagc catacagatt gatccaataa
    20341 cgcactttgg ggatagaaac ttcctccttc tcgcatcggg ccatccagca aataacttaa
    20401 tagatcgatc gcaatataat taagcattat agtaacgata atctcggatg tacctaattt
    20461 cgcttttaga taccctggga tcgccccgaa taaagctcca cctaaaaatc ccgcaatgat
    20521 acaaacaaga atttccagaa caggtgacgc cagagatatt tttagacctg taaatgttgc
    20581 ggcaatcgca ccgattatca tctgtccttc ggcgcccacg gaagttaaat tacatcgaaa
    20641 tgagattgct aatccgatgc ttgctatcag aattggtgtc gttctcaaca gtgtttcgga
    20701 aaatgcatat gaactgccaa atgctccgct gaacaagact ttaatcgctg cgaacggatt
    20761 tactcccgta atcgaaatgg ttagaccgct caacaaaagc gccatcaata ttgccaagaa
    20821 tgaactgatt gcttggattc ttcctttatt atgactgtgt tttgttacat tttttttatt
    20881 aatcaatggt ttcatcattc ttttccccgg ctctcttcac ttacttttga acctgccatt
    20941 aacaggccga tgtaaacaac agacgttttc ccgggaattg cttcccccat gatttttccc
    21001 tcgtaaatca ctaagatcct atctgaaatt gcaaggatct catccaaatc agaggaaatg
    21061 agcaaaatac ccatgttctc cgatcttttt tcaatcaata atttttgtac atattcggta
    21121 gcaccgatat ctaatcctct ggtcggttgt gccgcaataa gcagtttagg ctgtctgtat
    21181 atttctcttg ctaagaccac tttttgttga tttccaccgg ataattgttt taccggacta
    21241 ttaacattgc tgcatttgat ttcgtattga ttctttaatt tttcggcaac ccttacagcg
    21301 ttttgaggat caaaataaat tccgttcttt gtaaaaggcg gtttttcgca atttatcaaa
    21361 gcgatatttt ccgctacact aaaatcaagc accaatcctc tgatttggcg gttttccgga
    21421 atatgcgata agccgacttc gatcgcaaat ttcgaagaac aatttgttgt atctacagag
    21481 ttgatctcaa ttcttccgga agtcactttt cttaatccat ggattacttc tgccaattcc
    21541 atttgtccgt tcccgtccac tccggcgata ccgacgattt ccccttctcg aacttccaat
    21601 gagatattcg acaaagcgac taaccctctg tcattgttac agcaaacatc agacattttc
    21661 aggaccggtt taccgacgga gacagccggt cggtcatatt gaagaaaaac ttccctacca
    21721 accatcattt ttgcaagctg ttcccgggaa acatctttac tgtttacggt tgtaattttt
    21781 ttaccatttc tcaaaaccgt tatccgatca gagatattta gaacttcccc gagtttatgg
    21841 gaaataaaaa taaccgaatt tccttcttta acgatttcct tgagaacatt aaaaagatca
    21901 tccacttctt taggcgttaa aaccgcagtt ggttcgtcca taatcagaat attcgttccg
    21961 cgatataagg ctttaagaat ttcaacacgt tgctgttctc cgacagatag ttgcgaaact
    22021 tttgcccacg ggtcaatatc taatccgaac cgtgaagata aatcgactaa acgcttagcg
    22081 gccttttgca gatccaattt gagagcgtta tcggatttca tacaaagaat aatgttttca
    22141 ataacggata atgtcggaat caacatgaat tcttgatgga tcattccgat cccttttgcg
    22201 atagcatccg ctggggattt catgatgatt ttttccccat tcaaataaat ttcgccgctg
    22261 tcgggtgcat ataatccata cagaacattc attaaggtag atttaccggc tccgttttcg
    22321 ccgagaagcg catgtatttc ccctctattt acagacaaat ccacacaatt gttggcgata
    22381 acattttgga aagtcttcgt gatagcgcgc atttcaacaa ggggttgatt tgcagaagtc
    22441 gtttcttcat tttccataag cagacccatt atcattcgtt gaataaaaaa ccgtcggaga
    22501 ttgagttctc caacggcttt gaacataaac agaatttatt tctcgaaacc ggattttggt
    22561 aatatacctt gttccttaag cgagccgtct acaattccct gatagatttc tttcatctta
    22621 gcaattactt cgtccggaac cggttgatct cccatcgtat aaaaatccga aaggcgaatt
    22681 acgttatctt ttgcgcctac caaaacagtt tcgggtttga aatcatcaga gagagctgaa
    22741 gtaacgcttg catatacaag atcaccgatg tcttggattg ccgaaaaagg aaccgttccg
    22801 ggagcgatat catgttggtc ttcgatatag ccgattgcat aaattccttt tttctttgcc
    22861 gcatcaattg ttcccaacgc ggcttgaccg gtattggaaa caactacgtc gactccgctt
    22921 tcgatcatgg ccaaagtggc ttcacttcct tttgctatgt cggtccaact gtcgatatat
    22981 gaagtgacca cttcggcgtt cggattggca tatttagcgc ctgctgtaaa accatttgct
    23041 gcatccacaa ccggaggaag cattttgccg ccgatcatgc cgactttgtt ggtttttgtt
    23101 atcaaaccgg caaacgaacc cgctataaat ccgcattcgg gtgtattggg tctgaaatta
    23161 gcaagattcg gttcctgggc tccttcgcca tttacaatga caaatcggat attcgggtaa
    23221 tccggtgcga cactgattgc tgcttgatag aagtctgccg acgaaaaaat aatgatatcg
    23281 tatccttgtg cggcgtaatc tcgtgcggtc gattccatat cgaccaacgc aagattttct
    23341 gtataagcag tttcgattcc atatttttct ttcgctttca tcaatccttt gtatgatgat
    23401 tcgctccatc ccgcatcatt gattgttccc gttaacaata gcgctacttt ctttgtcgtt
    23461 cccgagcttt tttcatcttt ttcggaagtt tgatctgcga cggcggtgac agtaggagac
    23521 tcaaccggga tacttgttgc cgtaggaacc ggtgtcggtg tctgtttcga acatgctgta
    23581 aaagcaatca agataattaa caaaataaaa gcaaaactta aaatcttttt gaatctcatt
    23641 tcatcctcct tttggggact aatcctataa aattattaac tcatataatg actatatgtc
    23701 ttgcttgata ttatagcgac attattatta ttttgtcaat gatgaattta atgaggaatt
    23761 tttaatacct caaggcacag aaaaacaatt tcaagttgag aagaaatttt cgaaacagaa
    23821 aagagataaa aaaa
//
LOCUS       DAOSMM010000030        23667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2163_length_23667_cov_20.069753, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000030 DAOSMM010000000
VERSION     DAOSMM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 23667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23667
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2163_length_23667_cov_20.069753"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..3555)
                     /gene="mfd"
                     /locus_tag="PK898_05890"
     CDS             complement(67..3555)
                     /gene="mfd"
                     /locus_tag="PK898_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU19602.1"
                     /translation="MDQIKKIPGWEKYLELFKRKDRKTDFSSSGLIRAARYPVAAAVY
                     QELNRPVLYITDKPSHATLAFDELGFWLGEDSIMLFPAPDPLFYEQGNWDPATRSSRL
                     RTLLTLAERAIPSASAPANVPFIVTSVRGLMTRTMEKRNFVKSIRTLRINQQITQEEL
                     VRLWVQIGYEAVETVVEVGKFARRGGLLDIWPPDSALPVRIDFFGDEIDTIRTFDPAT
                     QRNLSSLERIQIGPAREFLFSEIPENPYFSSRPDEFLIPILHPQPSCLMDYLPENTLI
                     LADDWIRIQTLAEDIEEQAEKLRNQSIAENILPEDFPTPYVSFSEISDLLDSFQWHNL
                     SRGEEGSNLPIGEKFIPEERFGGKVNGFIDFLLDKSFNNEKVFVVSRQQQRLKSLWDN
                     LGAEAEIPEKPIILSGTISEGFKFTNREGQVVHFLTDSEIFGWERPIPRKVQQKTVDV
                     PEQYFADLKPGDYVVHIDYGIGRYSGLVSRSIDGVAKEFLCIEYAQGDQLFVPVHQAD
                     RLTNYIGPDGRAPELTRLGTQEWLSVKQHVSEKVVEVAEDLLDLYAKRQVTPGYAFSP
                     DTPWQMDMEGSFPYIETEDQKRAIRQVKEDMERPRPMDRLICGDVGFGKTEVALRAAF
                     KAACDGKQVAVMVPTTVLAQQHYDTFKQRFASYPVNVEMLSRFRTQKEQDQILKKLAE
                     GEIDVVIGTHRLVSADVKFKDLGLIIIDEEQRFGVAQKEYLKKMRTEVDVLTMTATPI
                     PRTLYMALTGARDISSINTPPDERVPIVTHVGPYSDKLVRQAIMREMDRDGQVFYVHN
                     RVQSIYAIAKHLQELVPEARIGIGHGQLPEKELSEVMESFYNREIDVLLSTSIIESGL
                     DVPNANTLIVDRADTLGLAQLYQIRGRVGRSSQRAYAYFFQQRGSHPTEEGLERLEVI
                     AENTQLGAGFSIAMRDLEMRGAGEILGNKQHGSIAAVGFNLYTRLLAQAVKKVKEDKG
                     LSISDEEIGITKDMAVLFNPITVELPMDIGIPEEYIPEQKTRIKLYRRIASIHEEEKL
                     IGLKDEFQERFGPLPEIVQNLFFQILVKIKAEKIGLSAVVKEGNSIVLRFSSFANEME
                     RGPLPEVGGGIRPGKNAYWLTLMNFQSDEWRNDLLVAMDLVEDRLAQQRIRSKEFGEQ
                     AEKSGVTSFLGIEKSP"
     gene            complement(3565..4203)
                     /gene="pth"
                     /locus_tag="PK898_05895"
     CDS             complement(3565..4203)
                     /gene="pth"
                     /locus_tag="PK898_05895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU19603.1"
                     /translation="MEHFFETIKKQREPVVESVGGEPYLLVGLGNPGREYRESRHNFG
                     FMVIDRIAETLRTEFKKSQMKALISVQAYEGKKVILSKPQTYMNCSGQAVIPLLNYYK
                     IPREHLLVIHDDMDLPFGTLRMRPGGGSGGQKGLESIIQLLGTQDFARLRCGIGHPPG
                     QMDVTDYVLNRFSKEESKLLPSVLENATKAALSFVSVGIQEAMTRFNGVVEG"
     gene            4628..6115
                     /locus_tag="PK898_05900"
     CDS             4628..6115
                     /locus_tag="PK898_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOU19604.1"
                     /translation="MRRRRNTLSILRWTSLALIFIAVLLTSFQLIRYSRMRSSFPPGL
                     TIGGVPVGGLNSSEAGERLVQAYGIPIELIYGNSLIQVRPSSVGFQINLETMLAAADL
                     QRITQSFWSGFWSYLWNTLPAPEPVPLSATVSKERLIAYLENEIVPRYDQPAKEPIPD
                     PGSAQFTEGVPGRELDIERSVVLITEALMSPTNRSVNLPYNRTMPARASFQNLQYLIR
                     QILVKNQFNGVAEFYLLDLNTRDELSFALSKGEQIPPDIAFTAASTIKIPIMTYVYIY
                     RDEPLGKTTEDDLKLMIEISENTPADRLLESIGGTLAPISFTEDLQKLGYKNTFLGGF
                     FYPGASLLARYETPANSRTDVNTSPDVYNQTTPAEISQSLDDIYQCANFNGGALKAVF
                     GDAIKQEECIKMLDMLSANKTAVLLEAGVPEGTKIAHKHGWILENDGLIHSISDAGIV
                     YTPNATYILTMFFYHPDQLVFDPTNIMASQISRAVYQFFNPEQPK"
     gene            6215..9772
                     /locus_tag="PK898_05905"
     CDS             6215..9772
                     /locus_tag="PK898_05905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19605.1"
                     /translation="MEETEQTQKQWLDRPVLNAFPAFSREKLIIALIILLTIISRFAI
                     LGNRVMSHDEVNHVVPSYSLYSGNGYVHDPITHGPLQFHLVALSYFLLGDNDFSSRVP
                     AAVFSIAAVIFVLFGFRKYLGRIGSLIGGFLFLISPFLLYYGRYTRNEAFIELFAVLT
                     FYGILRYFSKRDNFSLYLLAVVTALQFTTKEVAYIYTAQLLLFCGFVFLYDVWNFPWE
                     EIQKRTTAFALLLFGALIGAVGLLLLKYVSPESGADFRQYLGMGLLAIGALLVIIDII
                     SVIRQFGWQIIRKSPAFNLIVFIGALILPLLTAFPVRLMGWDPLDYSSAGIIHTGIVM
                     IVLFVISFLIGIWWNRDVFLKSLIAFYLIFITFYTTLFSNGHGFFTGMVGSLGYWLTQ
                     QDVQRGSQPLYYYAAILIPIYEYVAVAGSILGIFFAAKHHLFWAMPTDNLYELTEAKE
                     LPETNYAARVSEGDEIRADSFGDDENMDLHQTLSIDFIDNLESLHFEIEDNEPIEKIT
                     EIDELSSVQRQSTVLHQAPSFLLFLFWGLTALAAYSFAGEKMPWLTVHITLPLSLAAA
                     WGIGFLLEKAPWQKIASAKGLIALAALICGTFALSGLLSSLFGPNPAFAGKTLDQLKA
                     TNQFIFSAIAFLLVLFVLFRTWKGWSGKDILNSVVIYFVALLCILEARTAYTASFIHY
                     DYANELLVYAHAAPGPKIVLRQIEEIGARTGEGKSIKVAYDNDALYPYWWYFRDYPNK
                     YYFGEENPTRELRNYDVIIANTSKDSRLIPIVRDEYYRFETIRLWWPNQDYFNLTWDR
                     IRKSLGNPQMRSALFDIWLNRDYSQYAQVTGKNTFRPDNWDPSARMVVYMRKDLLKKM
                     YQLGDTALLSGSTTEEEGNVTEDDKFTQLDPVLSIGSEGTGPGQFNNPRNVAVAQDGT
                     IYVLDSDNQRVQYFSPSGEYLGMWDAKEQGGFNQPWGIAVSPEGEVFVADTWNHRIMK
                     FGSKGEFLLQWYANDPTEPNKTFYGPRALAVDTMGEIYISDTGNKRILIYSTDGAFIA
                     QFGTAGTGVGELDEPVGIAVWHDQLLAVADTWNQRVQIFDVSGEAPSFAVVGSFDIAG
                     WYSQSLDNKPYLAFDKDGNILVTDPEGYLVMQYSREGKLLRSWNGGGGDIDNFSMPTG
                     ITVDSEGAIWVVDTANDRVNKFVLPAADTEQDQLNNEKQ"
     gene            complement(9865..10821)
                     /locus_tag="PK898_05910"
     CDS             complement(9865..10821)
                     /locus_tag="PK898_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU19606.1"
                     /translation="MYTDSGLRIRKIKTRKDVLQIGELIENCFHPYLDPDGRRFVENL
                     QQIGKEYRDHPFFSLFRSFDLLLKGFVSINPEGKIDGVVTLFPCNVEYSFGYLIANVC
                     VAPESRHKGIATALMAFALHYAEKHHADTVYLQVREETPEAVRLYRKLNFTEDARRTI
                     WIRPKSDMQYPDEKVITFTEPNEQERTDFIHSFYKAYPEKILWNLNYVSDIFGMGALA
                     KIKRHLRKLESQFYQMQNADHQTIGWAAWQKTNSFTDTLWLVPTEACTGEEMTAALRK
                     LGDLYADKKAVSINFEKGRFTEAFEKAGFHKQGNSIWMSKKL"
     gene            complement(10811..11746)
                     /locus_tag="PK898_05915"
     CDS             complement(10811..11746)
                     /locus_tag="PK898_05915"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU19607.1"
                     /translation="MNIDLTVLQAFESIRTPALDAFFLLVTQFGEAAILIPALCIIYW
                     CFNKKQAFFLALNFFSALILNHILKITFCIERPWLRWQGEIAPVAKAKITATGYSFPS
                     GHVATATSVYGGIALWYRKNKKIRIAAIVLILAVSLSRIYLGVHTPSDVLASIFAGLI
                     LLFLNLRIFNRIAANPEDDKKFLLVSLFVLILGLIYTFYKSHWNPSMEILWLDGIETM
                     GTAAGVLTGWYWEEHRIRFQPIRRVGPAMIVVTVGLAGTFLILSVIGKFLKAIFGILS
                     GNFLCYFLIGFWIIGLYPYLFNKILNPVSDEKYVH"
     gene            11956..13338
                     /gene="lpdA"
                     /locus_tag="PK898_05920"
     CDS             11956..13338
                     /gene="lpdA"
                     /locus_tag="PK898_05920"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU19608.1"
                     /translation="MNAYDLIVIGAGPGGYIAAIRGSELGLKTAIVEKDSLGGVCLNV
                     GCIPSKSLLKNAELVRTLRKESETFGIRASDLSFDYAAAVDRSRKVSKRLNQGVAFLM
                     KKNKIDVYNGKASFTDPKHVRVSLNSGEEEFLEAADFIIATGTVPTELKGMEFDGERI
                     LNYRQAILQTKLPNSVVIIGGGAIGVEFATIWSAYGSAVTVVEMQPNLLPNEDPEAGR
                     ELERSFKKQGIDVKTGTRLKSIEKVSDGVMIHLINEKGESEVSAQQVLIAVGFRPNPA
                     ELNLEAAGVKTTERGWIDCDPFGKTAADHIYAVGDITGKILLAHSAMTMGRIAAEQIG
                     GLITEPLNLDLVPHCTFSFPQVASFGLTEASADARKIKIKAGKSTFLANGKALGMNEN
                     EGWIKLICDATDHRILGATMIGAEVCELLPELTLAATKQLTAEDILMNIHAHPTLSEG
                     IAEAAGNLLI"
     gene            13381..14328
                     /locus_tag="PK898_05925"
     CDS             13381..14328
                     /locus_tag="PK898_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOU19609.1"
                     /translation="MRPEISVLSIILIIEAVWWLVWILLALIRKKFSVFAALLGTLLI
                     WGLSGLFLVSSAPEGGPMTGQLVGLVILAIIPFIMLPPMVVIINEYQRGVLFRLGRLM
                     ATLEPGLNIIFPFGIDRVVKIDLRTFTIDVSKQEVITKDNVPVLVDAVVYFNVFDPIL
                     AVTKVANYTLSTTLLGQTSLRSVLGQHELDEILSKRTELNEVLRKLLDEDTDPWGIKI
                     SAVEIKSIELPDTMKRAMAKQAEAERERRAKIIAADGEFQAAQKLLDAASVISKDPAT
                     LQLRYLQTLSEIAVERNSTILFPLPMELLKAFGNKQAPE"
     gene            complement(14417..15493)
                     /gene="ychF"
                     /locus_tag="PK898_05930"
     CDS             complement(14417..15493)
                     /gene="ychF"
                     /locus_tag="PK898_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOU19610.1"
                     /translation="MRLGIVGLPQSGKTTLFNALTRGSFPVGTATGKIEIHDAVVDVP
                     DERVDVLAKMFHPKKTIYAKVNYADIAGLDGSSAKSGISGELLNKLAQMDGFIHVVRC
                     FTNDQVPHPAGSIDPIRDIQTLDGDFLLNDLISVEKKLERLTDEKRKGGGREKAVIDR
                     EIELFEKLHACLAEEKPLRELDLTAEEEKAISGFGLLSRKPVLILLNLDETQEVPRID
                     YPYKQSKVESIRGQIEMELAQLSPEDAAMFMEDYHISELGLNRVIRLSYELLGQLSFF
                     TAGADECRAWTVHRGATAPEAAGVIHTDLQKGFIRAEVIAYDDLIELGGMNEAKAKGK
                     LRLEGKDYIVKDGDILNIRFNVSK"
     gene            15546..15626
                     /locus_tag="PK898_05935"
     tRNA            15546..15626
                     /locus_tag="PK898_05935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15580..15582,aa:Leu,seq:caa)
     gene            15984..16733
                     /locus_tag="PK898_05940"
     CDS             15984..16733
                     /locus_tag="PK898_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19611.1"
                     /translation="MNPSRFGLILIIATLLIYGLCLFLRNKIKLLPLYKNDSNSKKLQ
                     LSLSRSIEEGKPIHLDVLNRDESQLIAPSALVAINTSEQLANRFAASDEPPFVTNNSG
                     LVHALTKDAVKMGYRQADLENEFSAASLDFAGFDSLSHQTTMLGLLEETPYAMHLNIG
                     SGGPEIALQDMMFQNEEEIYIAGDSLLGQATGLATADDVFIGEQLYELPVITQPERKE
                     AAPDVGLLVMDILRYGFIVALGIGALMILLV"
     gene            16747..17385
                     /locus_tag="PK898_05945"
     CDS             16747..17385
                     /locus_tag="PK898_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19612.1"
                     /translation="MDLSSKNKRLTVLAGLIGIFFLISAYSDQPAVRTARTMLIHWAS
                     LVAAVLLFIAIIDFVIARIRGIKDHTGSFIYNLISLLIFLIVFFYGFSNQAGASSVQS
                     TVFMIQSSVESVMTGLICLVMIYGIYRVARSQRSLLKSSFLFSTIFFLVLYSGFFSFL
                     KLPAFLSKIIDFIRILPVGGLYGLLIGIAIGALVTGIRVLFFGDHPYQGHKS"
     gene            17382..19883
                     /locus_tag="PK898_05950"
     CDS             17382..19883
                     /locus_tag="PK898_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19613.1"
                     /translation="MRTFKCPICGSSNEENAKTCAVCLVNFSQLPEDLQLQIQHELEN
                     QQEAVPLQEDEKNDRQNEVPDWLRRRIRPQSDEKENKSFDNYLDLIFHKSVEENQSSA
                     DLNEKSEGDSFPFTQEDFEALEIKEDGVPQETELSPEIEESLSDFSSVRPAQKWDEAS
                     SDNEIVDGKKIVSSDSENEFVDFSKSRPAKKWEDLTPVPNEKIGISGKRETTHPESFE
                     SAKFVSEQKRDKTPLADQKNENIKINSLPEESFVDFSVVRPQQKWDSGQESDAPSVFS
                     VDESSHDVFRDEQLTFFENKSAEPQDVNSELTEEEALYADFSVNRPEKKWDDGNELNS
                     PTADSSSSLETTETVSNKQPFSVTENENSAGKNAVSEEAGLVDSFLRQIAAGTETPQE
                     PIQESAAMTSPEWVTPPEPAPQPSSGIDEELEFLMENPAPSAAEVREEAVTGQNQEEP
                     SVSESVPEEPEPPVSSEGWTQDEIVPDELEFEEDDLDEVPWNLFETGEMSLPGLEPAE
                     IAYKPFSKSGIQEGKNNQDYQQRMVMTILDKIFQIEGLNKPFVKNQNRGTRKNTQFLI
                     GLVMILGILAILFTGITDSIPLNPPDPESFPNLVTFNREVDKLTNNDHVLVVVDYIPG
                     FENEMNEPVTALLKNITDKKARISLVTTNLSSAAVTAKLKAEIDPTGIEDIGYQTGGV
                     LAIQSVLQNDLSPYTTVYLVSSGFESARTWLEQIASMSVPLPVNVIASTQLESLLQPY
                     YESKLINSYLTGKMEKEIFSGFTFDDPIRNRRLFALWFLIFTLILAFLAGNVQRTPYH
                     TIIVEEKPKSRPPKPSTSDKTENKIDKGIKNDIAF"
     gene            19867..20565
                     /locus_tag="PK898_05955"
     CDS             19867..20565
                     /locus_tag="PK898_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19614.1"
                     /translation="MTSPSDFTVLLIGFLLSVFTFSLILGDNWLFRLSSAVLSGALAG
                     YLINVLIEKIFIPSIVNPLMESNVDWKNQALAVVVIISAVFLFLKYYFRSPAGGNAIL
                     AVLLCSAAAITILGIVNGTLINLYRGLIARFLPGAEMSSANIYWVKTGIITLGVISSL
                     IYTQHYAFGKKNGKEISRQRNTVFFFRTLGEVFIGIAMGAVFAGCFIASSTILIGQIS
                     NLVGSGQEILQWVK"
     gene            20567..22327
                     /locus_tag="PK898_05960"
     CDS             20567..22327
                     /locus_tag="PK898_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOU19615.1"
                     /translation="MNRNQDIHTVLYVDIGSVNTRVCLFDRMDDQYSFIGAASATTSG
                     DITNQFEYLNVIQAVEKLEHATKRKILDRNQNIVMPATLDHSGIDQVAVSYTCIPDPR
                     IAVMGLTHAGSLQNVYDLLSKVGVKPVAEICAEDGKTVCENLDLFLHTAPRIVIVSGG
                     TEQGAEIGIYRLGEILMLACRSLPKEQRPIILYAGNSAAKKQMERVFSQITDISFSTN
                     VLESGSGVQPTLNGLINVLRKDLESRAPGFRILREKTKCSVIPGEFAFGRTIRLMSRL
                     IRQNRHILGIDIGAHQTILADAVNLALSLHTLPLGIGNNIGNLLNEYTPSDLQSWISF
                     QIKSAEIQDYILNKSYYPDLIPANKYSAEIELALARAIVMKGIENESLSAMLSDGSLA
                     MVFLSGAVLRNVEDPGEALMFAMDSLAPVGMVDYLLDMNGLSGAIGTLAHLNPVLASQ
                     IMSTSAYLNLGKVIRPSVRGEPPGKSIMTVSVRDDAGNTRKYDIPFNRIYRIPLEYGK
                     NYELEWTKISHNVTVPGVKTWAPAGFKSGCFGLVFDTREEKDGKIKLPKEKAARDKKL
                     QSWRTELGPWLVKQEEESHA"
     gene            22320..23396
                     /locus_tag="PK898_05965"
     CDS             22320..23396
                     /locus_tag="PK898_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19616.1"
                     /translation="MPEEAVHIISSGTVRCARLLSEDGTVVVFKGQMADPTTQIAFRK
                     IWSRHFSLNILDALDLDPADNIREFLTIKEGDFVSVGEQIAKREGRRKLSVTAKASGR
                     FLGISANRLIFESDPVDIESRVAGFPGVVTDIVNNRGAYLETSGSYIKGLWGNGKCGQ
                     GILLSIDMEKNNGIFDLDSISVDLAGAVVFANTCTDANVLKTAAKMTPGGLIFGSLPS
                     YLLPVAVSLPFPIIVTDWIGTGKISNPVHLTLGENIIKFAYLYSDNSASGKLIRPEII
                     IPQDEYIPEKENPLNHLGIGSQVRIIEGFYGGELGIVVDILPGNDENSDNTEIAPDQV
                     KICIDEETYVTLPKNNVEVINLKS"
     gene            23411..>23667
                     /locus_tag="PK898_05970"
     CDS             23411..>23667
                     /locus_tag="PK898_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19617.1"
                     /translation="MLDFDDKELLEGFEEEFKKKKRNKRSGNRFPSWIIIAIILVAGV
                     FFFLYKKPDTVKVQTNKTPTVELTEGQQSLIQTRQISSSTV"
BASE COUNT         6436 a         5478 c         5316 g         6437 t
ORIGIN      
        1 caaggttctt cgcgcccctc tgtttcccag gcgcaacgtc ttattatatg tgttttcccc
       61 tttatgtcaa ggactttttt caattcccaa aaaactcgtt acgccggatt tttctgcctg
      121 ttccccgaat tctttagacc ggatcctctg ctgagccaag cgatcctcga ctaaatccat
      181 agcgaccaac aggtcgtttc gccattcgtc cgattggaaa ttcatcagcg tcagccaata
      241 agcatttttt ccgggtcgga tccctcctcc gacttccggt aacggacctc tttccatctc
      301 gttggcgaag gatgaaaagc gcaacacgat gctgttcccc tcttttacca ctgcggataa
      361 accgattttt tcggctttta tttttacgag aatctgaaaa aatagatttt gcacaatctc
      421 gggcagcggt ccgaatcgct cttgaaattc atcttttaat ccgatcagct tttcttcctc
      481 atgaatagat gcaatccgcc gatacagttt aatccgggtc ttttgttcgg gaatatattc
      541 ttcagggata ccgatatcca tcggcagttc taccgtgatc gggttaaaca aaaccgccat
      601 gtcttttgtg ataccgatct cctcatccga tatcgaaagt cctttatctt ctttgacttt
      661 cttcacagcc tgtgctaaaa ggcgggtgta caaattgaat ccgaccgctg caatcgaacc
      721 atgctgtttg tttccgagaa tttcccccgc accgcgcatc tccaaatcgc gcatcgcaat
      781 cgaaaaaccc gcgcccaact gagtattttc ggcaattact tccaatcgct ccaatccttc
      841 ctcggtcgga tggctgccgc gttgctggaa aaaatatgca taggcgcgtt gggagcttct
      901 gcccactcgg cctcggatct gatagagttg agccaatccc aaagtatccg cccggtcgac
      961 aatcaaagtg ttcgcattgg ggacatccaa gccggactca ataatcgatg tactcaacaa
     1021 cacgtcaatt tcccggttat agaaagattc catcacctcg gacaattctt tctcgggcaa
     1081 ttgtccatga ccgattccga ttcgggcttc cgggaccaat tcctgcaggt gttttgcaat
     1141 cgcataaatg gattgaacac ggttgtgaac gtaaaatacc tgtccgtccc gatccatttc
     1201 ccgcataatc gcttgccgga ccagtttatc cgaataagga ccgacatggg taacgatggg
     1261 cacccgctca tcaggcggtg tgttgatact ggagatgtcg cgggctcccg tcaacgccat
     1321 atagagcgtc ctgggaatcg gcgtagcggt cattgttagt acatcgactt ccgtccgcat
     1381 ttttttcaaa tactcttttt gagccacgcc gaaacgctgc tcctcatcaa taataatcag
     1441 acccaaatct ttgaatttta catccgccga aaccaaacga tgagttccga tcaccacatc
     1501 aatttcccct tcagccaact tctttaaaat ttgatcttgt tctttctgcg tccgaaagcg
     1561 ggaaagcatt tccacgttga ccggataaga ggcaaaacgt tgcttgaagg tatcatagtg
     1621 ttgttgggct aaaaccgtcg tcggaaccat tactgccacc tgttttccgt cacaggcggc
     1681 tttgaaagct gctctcaagg caacttccgt cttaccgaaa ccgacatctc cgcaaatcaa
     1741 acgatccatc ggccgcggcc gttccatgtc ttcttttact tgacggatag cccgcttttg
     1801 atcttcggtt tcaatatagg ggaaagaccc ttccatatcc atctgccaag gggtatccgg
     1861 cgaaaatgca taacccggtg tgacctgtcg tttggcatac aagtccaaca aatcttcggc
     1921 cacttccacc actttttcac tgacatgctg tttaacgctc agccattcct gcgttcccaa
     1981 tcgggtcaat tcgggcgccc gtccgtcggg accgatatag tttgtcaacc gatcggcttg
     2041 atgaaccggc acaaacagtt gatctccctg agcatattca atacataaaa actctttggc
     2101 gacaccgtca atggatcggc tgaccagtcc ggaataccgc ccaattccat agtcgatatg
     2161 gacgacataa tctcccggct ttaaatccgc gaaatactgt tcgggaacat caaccgtctt
     2221 ctgttgaact tttcgaggga tcggtcgttc ccaaccgaaa atttcgctat ccgtcaaaaa
     2281 gtggacaacc tgtccttctc ggtttgtaaa tttaaaaccc tcactgattg ttccggaaag
     2341 gatgatcggt ttttccggta tttccgcttc ggcacccagg ttatcccata aggatttcag
     2401 tcgttgttgt tggcggctga caacgaaaac tttctcgttg ttgaacgact tgtcaagcaa
     2461 aaaatcgata aatccgttta ctttaccgcc gaagcgttct tccggtataa acttttcccc
     2521 gatcggaaga ttgctgcctt cttcgccacg ggagagattg tgccattgaa aagaatccaa
     2581 cagatcgcta atttcggaaa agctgacata aggagtcgga aaatcttcgg gaagaatatt
     2641 ctccgcaata ctctgatttc gaagtttttc ggcttgctct tcgatgtctt ccgccagtgt
     2701 ttgaatacga atccaatcat ccgccaatat cagcgtattt tccggcaagt aatccatcag
     2761 gcaggaaggc tgcggatgaa gaatcggaat caaaaattca tcggggcggg aagaaaaata
     2821 aggattttcc ggaatctcgg aaaacaggaa ctcccgtgcc ggaccgatct gaatacgttc
     2881 cagtgaggaa agattccgct gtgtagccgg atcaaaagtc cgaatggtat cgatttcatc
     2941 cccgaaaaaa tcaatccgaa cgggaagagc cgaatccgga ggccaaatat ccagcaagcc
     3001 gccgcgtctt gcaaatttgc cgacttccac gacggtttca accgcttcat aaccgatttg
     3061 aacccaaaga cgaaccagtt cttcctgagt gatctgttga ttgattcgca gtgtccgaat
     3121 ggatttaacg aagttccttt tttccatggt tcttgtcatc aatccgcgga ccgatgtcac
     3181 gataaacgga acattcgcag gtgcggaagc cgacggaatc gcccgttcgg cgagtgttaa
     3241 aagtgttctc aagcgcgagg aacgggtagc cggatcccaa tttccctgtt cgtaaaataa
     3301 cggatcaggc gccggaaaca acattattga atcttccccg agccaaaagc cgagttcgtc
     3361 gaacgccaga gtcgcatgcg acggtttatc cgttatgtag aggaccggtc ggtttaattc
     3421 ctgataaacc gcagccgcca ccgggtaacg ggcagcccga atgagaccgg aagaactgaa
     3481 atcggttttc cgatccttcc gtttgaatag ttccagatat ttttcccaac cggggatttt
     3541 cttaatttga tccaacaacc tgctttatcc ttccacaacc ccgttgaatc gggtcatcgc
     3601 ttcctgaatt ccgacggata caaaagaaag cgctgctttg gtcgcattct ccaaaacaga
     3661 tggcaagagt ttcgattcct ctttcgaaaa ccgattcaac acataatccg tcacatccat
     3721 ttggcccggc ggatgtccga tgccgcaacg taagcgagca aaatcctgag tccccaaaag
     3781 ttgaataatc gattctaagc ctttttgtcc tccggaaccg cctcccggac gcattcgtaa
     3841 ggttccgaac ggcagatcca tatcatcgtg aatgaccagt aaatgttctc tgggaatttt
     3901 gtaatagttc aataacggga tgactgcctg acccgaacaa ttcatatacg tttgcggctt
     3961 tgataaaatt acttttttcc cctcataagc ttgaacggaa atcaaagctt tcatttggct
     4021 tttcttgaac tccgtccgga gcgtttccgc aattcgatcg atcaccataa atccgaaatt
     4081 atggcgcgat tcacgatatt cgcgtcccgg gttgcccaat ccgaccagca aataaggttc
     4141 gcccccgacc gattcaacaa caggctcccg ttgttttttt attgtttcaa agaaatgttc
     4201 cataggtctc gtcattttct gtaatctttg ccataacccg aagaggcagg gagttcggaa
     4261 tgccgtaaga tttcatcgat atcgataact tcgcagatca tgaataatct ttacaataaa
     4321 gaaaattagg attacgaaga caatccctaa aaataccttc aatcccaaac tcatcaagtc
     4381 caccgtcgcc gggtttctta acccttccgg tggaacttcc gtcaatatcg ccgattccgc
     4441 taacacttcc cgcctttctt ccgattgtca tccgttatca taacaaccgt aacattttag
     4501 tttaacatga gcaggccgaa gctgcaaatt ttaccgaaaa aaccatgatt tattgatcaa
     4561 aatctcgaca aatcatcctt aaccttaaag aaaaccgaat caatttactg ctggtaaaat
     4621 tgtctgtatg agaaggcgaa gaaatacgtt aagcattctg cgatggacct ctctggcatt
     4681 aatcttcatc gcggtcttgc tgaccagttt tcaattaatc agatacagtc gaatgcgttc
     4741 ctcatttcct cccggcttga cgatcggcgg tgtgccggtc ggcggactga actcatcgga
     4801 ggccggggaa cgtttggtcc aggcttatgg tatccccatc gaactgatct acgggaattc
     4861 cttgatacag gttcgtccgt cttcggtggg ttttcaaatt aatctggaga ccatgttggc
     4921 agcggccgat ttacaacgga tcacacagag cttttggtcg ggattctgga gctatctttg
     4981 gaatacactt ccggctcccg aaccggttcc cctttcggca acggtttcca aggaacggct
     5041 gattgcttac ttggaaaatg aaatcgtccc ccgctatgat caaccggcaa aagaaccgat
     5101 ccccgatccg ggcagcgctc aatttacgga aggagtcccg gggcgcgaat tggatatcga
     5161 acgttctgtc gtcttgatca cggaggcact catgtctccg acaaatcgaa gcgttaattt
     5221 gccttataac cgtacgatgc cggctcgggc ttctttccaa aatttacaat atttaatccg
     5281 tcagattctg gtgaaaaatc aattcaacgg cgtggcggaa ttttatttgc tcgatctgaa
     5341 taccagagac gagctcagct ttgcgctttc caaaggcgaa cagataccac ccgatatcgc
     5401 ttttaccgca gccagcacca ttaagattcc gattatgact tatgtctata tctaccgcga
     5461 cgaaccttta ggtaaaacaa cggaagatga tttgaaactg atgatcgaaa tttcggaaaa
     5521 tactccggcg gatcgcttgc tggaatcgat cggcggcact ttggcaccga tttcttttac
     5581 ggaagatctg caaaaattag gatataaaaa cacttttttg ggaggcttct tctatccggg
     5641 tgcctctttg ctcgccagat atgaaactcc cgcaaactct cgcacggacg tgaacacatc
     5701 ccccgatgtc tataaccaaa cgacacccgc ggaaatatcc cagtcgttgg atgacattta
     5761 tcaatgtgcg aatttcaacg gaggagccct gaaagccgtc ttcggagatg cgatcaaaca
     5821 ggaagaatgc atcaaaatgc tggatatgct atcggcaaac aaaacggccg tgttacttga
     5881 agcgggtgta ccggaaggca ccaaaatcgc ccataagcat gggtggatcc ttgaaaatga
     5941 cggattaatc cacagcatca gcgatgcggg aatcgtctac acacctaacg ctacctatat
     6001 tctgacaatg ttcttctatc atccggatca acttgtcttt gatcccacca atatcatggc
     6061 ttctcaaata tcgcgtgcgg tctatcagtt tttcaatccg gagcaaccga aataagaaaa
     6121 cggttaagct ataatcaccg atatacaaat gaaaaattca atctgttctt tccggcggaa
     6181 aaaatcagac aaaacttgaa aggttttgaa atttatggaa gaaactgaac aaactcaaaa
     6241 acaatggttg gatcgaccgg ttttaaatgc atttcccgca ttctcaagag aaaaactgat
     6301 cattgcgttg attatcttat tgacaataat cagccgtttc gcaattctcg gaaaccgggt
     6361 catgtctcat gatgaagtca atcatgtggt tccttcctac agtttatatt ccggtaacgg
     6421 ttacgttcat gatcccatta cgcacgggcc gctccaattt cacctggtgg cgttatccta
     6481 ttttttgttg ggtgacaatg acttttcctc cagagtaccg gccgctgttt tcagtatcgc
     6541 ggcagttatc tttgtcctgt tcggttttcg aaaatatctg ggacgaatcg gatcgttgat
     6601 aggaggattt ttatttttaa tttctccttt cctgctctat tacggtcgct acacccgtaa
     6661 cgaagctttt atcgaactct ttgcggtctt aacattctac ggaattttac ggtacttttc
     6721 caagcgagat aatttttcgc tatatttatt ggcggtggtc acggcgcttc agtttacaac
     6781 aaaggaagtt gcctatattt atacggccca actattgttg ttctgcggtt tcgtttttct
     6841 ttatgatgtt tggaattttc cttgggaaga gatccaaaaa agaacgacag cattcgcttt
     6901 attgcttttc ggagcattaa tcggcgcggt cggccttctg ctgctgaaat atgtttcacc
     6961 ggaatccggc gccgatttcc ggcaatatct cgggatgggg cttctggcga tcggcgcttt
     7021 gctcgtgatt atcgatatca tttccgtcat tcgtcaattc ggttggcaaa tcatccgtaa
     7081 gtcgcccgct tttaacttaa tcgttttcat cggtgcctta attctgccgt tattaaccgc
     7141 ttttccggtc cgattgatgg gctgggatcc gctggattat tcctccgcgg gtattatcca
     7201 cacgggaatc gtaatgatcg ttctttttgt tatttctttc ctgatcggga tctggtggaa
     7261 tcgggatgtt tttctaaaat cattgatcgc tttttatttg atttttatca cattctatac
     7321 gactctgttt tccaacggtc acggcttttt taccggaatg gtcggttcgt taggatattg
     7381 gctgacacaa caagatgttc aaagaggcag tcagcccctt tactattatg cggcgatttt
     7441 gattccgatt tacgaatatg tcgcggttgc aggcagtatt ttagggattt tcttcgctgc
     7501 caagcatcat ttgttttggg caatgcccac cgataatctt tatgaactga cagaagcaaa
     7561 agaattgccg gaaacgaatt atgccgctcg ggtttcggaa ggggatgaaa tacgggcaga
     7621 ttctttcggc gacgatgaga atatggattt acatcagact ctgagcatcg atttcatcga
     7681 taatttggaa tcgctgcatt tcgaaatcga agacaatgaa cctattgaaa aaataacgga
     7741 aatcgacgaa ctaagcagcg tccaaagaca atcaaccgtc ttgcatcaag ccccgtcatt
     7801 cctattattc cttttttggg gactgactgc gttggcggct tattcttttg caggtgaaaa
     7861 aatgccgtgg ctcacggttc atatcaccct tcctctgtcg cttgccgctg cttggggaat
     7921 cggcttcttg ttggaaaaag ctccttggca gaaaatcgct tccgcaaaag gtctgattgc
     7981 cttggcagct ttaatttgcg gaacctttgc attgagcggg ctcctctcat cgttattcgg
     8041 accgaatccg gctttcgccg ggaaaacatt ggatcaatta aaggccacaa atcaattcat
     8101 tttttcggcg atagcttttc tgcttgttct ttttgtcctg ttcagaactt ggaaaggttg
     8161 gagcggtaaa gatattctga attccgtcgt tatttatttt gtcgcattgc tttgcattct
     8221 cgaagcgaga accgcttata cggcgtcatt tatccattat gattatgcca atgaactgct
     8281 ggtttatgcc catgccgcac ccggtccgaa aattgttctg aggcaaatcg aggaaatcgg
     8341 agcgcgaacc ggagagggaa aatccataaa agtggcatac gataacgatg cgctgtatcc
     8401 ttattggtgg tattttcggg attatcccaa taaatattat ttcggcgaag aaaatccgac
     8461 acgtgaactg cgaaattatg atgtaataat cgccaatact tccaaagaca gtcgtttgat
     8521 accgattgtc cgagatgaat actatcgatt tgagacaatc cgcctgtggt ggccgaatca
     8581 ggattacttc aatctgactt gggatcgaat ccggaaaagt ctcggcaacc cgcaaatgcg
     8641 ctccgcgctt tttgatatct ggctgaacag ggactacagc caatatgcgc aagtaaccgg
     8701 taaaaatacc ttcagacccg ataactggga cccatccgca cggatggttg tatacatgcg
     8761 aaaagattta ttaaagaaaa tgtatcaact cggtgatacc gctcttctgt ctggctcgac
     8821 cacggaagaa gagggaaatg ttaccgagga cgataagttc acccagctgg atccggtttt
     8881 atcaatcggt tcagaaggaa ccggcccggg acaattcaat aaccccagaa atgtcgccgt
     8941 cgctcaagat ggtacgattt atgtattgga ttcggataac caacgcgtcc aatatttctc
     9001 cccaagcggc gaatatttgg gaatgtggga cgcaaaggag caaggcggtt ttaatcagcc
     9061 gtggggaatc gccgtcagcc ccgaaggtga agtttttgtg gcggatacct ggaatcatcg
     9121 aattatgaaa ttcggttcga aaggtgagtt cctgttgcag tggtatgcaa atgacccgac
     9181 cgaacccaat aagacattct atgggccgag agctttggct gtcgatacaa tgggagaaat
     9241 ttatatctcc gacaccggaa ataaacgcat cctgatttat agtacggacg gtgctttcat
     9301 cgcacagttc gggactgccg gaacgggcgt cggggaattg gatgaaccgg tgggtatcgc
     9361 cgtctggcat gatcaacttt tggcggtcgc ggatacctgg aaccaacggg ttcaaatttt
     9421 tgatgtcagc ggtgaagctc cgagcttcgc ggttgtcgga tcgtttgata tcgccggttg
     9481 gtattcgcaa tcgttggaca ataaacctta tcttgcgttt gataaagacg gaaatatcct
     9541 ggtcacggat ccggaaggat atttggtgat gcaatactct cgcgaaggaa aactgcttcg
     9601 ctcatggaac ggcggcggcg gtgatattga taacttttcg atgccgaccg gaataacggt
     9661 tgacagtgaa ggcgcaatct gggtagtcga cacggcaaac gatcgtgtca ataaattcgt
     9721 tttacccgca gcggacacgg aacaagatca attgaacaac gaaaagcaat aatttaaatc
     9781 tctgccgtcc gaattctcgg taaaggggaa ttcggacggc agagaaaagg caaaatgtga
     9841 atctttcaat cgatccgaat caattcaaag tttcttgctc atccaaatag aattcccctg
     9901 tttatgaaaa ccggcctttt caaatgcttc ggtaaatctg cctttttcaa aattaatcga
     9961 aacagctttc ttgtctgcgt ataaatctcc cagctttctt aatgccgccg tcatttcttc
    10021 tccggtgcag gcctccgtcg gaaccaacca taaggtatcc gtaaatgaat ttgtcttctg
    10081 ccaggcagcc caaccgatcg tctgatgatc ggcattttgc atttgataaa actgagattc
    10141 cagttttcga agatgccgct tgatttttgc caaagcgccc atcccgaaaa tgtccgaaac
    10201 ataatttaaa ttccacagaa ttttttcggg atatgcttta tagaaagaat gtatgaaatc
    10261 agtccgttcc tgttcgttcg gttccgtaaa cgttattacc ttttcatccg gatattgcat
    10321 atccgatttc ggtcgaatcc aaatcgttcg acgggcatct tcggtaaaat ttaatttccg
    10381 ataaagccga accgcttcgg gtgtttcttc ccggacctgc aggtaaactg tatcggcatg
    10441 gtgtttttcc gcataatgga gcgcaaaagc catcaaagcg gttgcgattc ctttatgacg
    10501 tgattccgga gccacacaga cgttggcaat taaatacccg aacgaatatt cgacgttaca
    10561 gggaaaaagc gtcaccacac cgtcgatttt tccctccgga tttatactga caaatccttt
    10621 aagcaacagg tcgaaagatc ggaacaaact gaaaaaagga tgatcgcggt attcttttcc
    10681 gatttgctgc aaattttcaa caaatcgtct gccgtccgga tccaaatagg gatgaaagca
    10741 attctctatg agttcaccga tttggagcac atcttttcgg gtttttattt tgcggatacg
    10801 gagtccggaa tcagtgtaca tatttttcgt cggaaaccgg attcaatatc ttgttgaaaa
    10861 gatacggata gagtcctatg atccagaaac ctatcaggaa ataacacaaa aaattccccg
    10921 ataaaattcc gaaaatcgct ttcaaaaatt taccgataac agataagatc agaaacgttc
    10981 ccgccaaccc gacggtaaca acaatcatcg ccggccccac ccgacgaatc ggctgaaaac
    11041 gaatccgatg ctcttcccaa taccagcccg tcaaaactcc ggcagcggtc cccattgttt
    11101 cgataccgtc cagccataag atttccatcg acggattcca atgagattta taaaacgtat
    11161 agataagccc gagaatcagg acaaaaagcg aaaccagcaa aaacttttta tcatcctccg
    11221 ggtttgcggc gatccgatta aaaatcctta aattcagaaa aagaagaatt aatccggcaa
    11281 aaatcgaggc caaaacatcc gagggggtat gaacacctaa ataaattcgt gacagactaa
    11341 ccgcgagaat caaaacaatt gccgcaatcc gaatcttttt attttttcga taccaaagtg
    11401 cgatcccgcc atagacactc gttgccgtgg caacgtgacc gctgggaaat gaatagccgg
    11461 tagctgtgat ttttgctttt gccaccggcg caatttcccc ctgccaccgc agccatgggc
    11521 gttcgataca gaaagtgatc tttaaaatat ggttgagaat gagagccgag aaaaaattaa
    11581 gcgccagaaa aaaagcctgc tttttgttga aacaccagta aataatacac aatgccggaa
    11641 tcagaatggc tgcttctcca aactgtgtga ccagtaaaaa aaaggcgtca agcgccggag
    11701 ttcgaatgga ttcgaaagct tgcaaaacgg ttaaatcgat attcatgccg gttctcttct
    11761 cttaaaaatt tattctaaat tataacgaac agaatcggag aaatttacaa ttaaggtgct
    11821 aaaaaaagcg aattctgtcg tcctgaatcg tcaaaatctc atatttacgg caaaattatt
    11881 ataatcatac ccatgagatc ccgattccgg gtatttgatg ccatattaaa aaagattagc
    11941 aaaggacaaa atcaaatgaa tgcatatgat ctgatcgtta tcggtgccgg ccccggagga
    12001 tacattgccg cgattcgcgg ttctgaactc ggactgaaaa ccgccattgt tgagaaagac
    12061 agtttgggag gagtctgtct gaatgtcgga tgtattccct caaaatcttt gctcaagaac
    12121 gccgagttgg tgagaacact gcgaaaagaa agcgagacct tcggtattcg tgcgagcgac
    12181 ttgtcttttg attacgcagc ggcggttgat aggagccgaa aagtttccaa gaggctcaat
    12241 cagggcgtcg cttttctgat gaagaagaat aaaatcgacg tctataacgg gaaagcctct
    12301 tttacggatc ccaaacacgt ccgcgtttcg ctcaattccg gagaagagga atttcttgaa
    12361 gcagccgatt ttattatcgc tacgggtact gttccgaccg aactgaaagg aatggagttt
    12421 gacggtgaaa gaatcttaaa ttatcgtcag gcgattctcc aaaccaaatt gccgaattcc
    12481 gttgtaatta tcggcggcgg agcgatcggc gtagaatttg ccacgatatg gagcgcgtac
    12541 ggttccgcgg ttaccgtcgt ggaaatgcaa ccgaatttac tgccgaatga agatccggaa
    12601 gccgggcgtg aattggaacg atctttcaaa aaacaaggaa tcgatgtaaa aacaggcacc
    12661 cgcttgaaat caatcgaaaa agtctccgac ggcgtgatga ttcatttgat caatgaaaaa
    12721 ggtgaaagcg aagtgtcggc gcaacaggtt ttgatcgctg tcggatttcg accgaatccc
    12781 gccgagctca atctggaagc cgccggagta aaaaccaccg aacggggatg gattgattgc
    12841 gatccgttcg gaaaaaccgc tgcggatcac atctatgccg tcggagatat taccgggaaa
    12901 atcttactgg cgcattcagc catgacaatg ggaagaatcg ccgcggaaca aatcggcggt
    12961 cttataaccg aaccgttgaa tttggatttg gttccgcatt gcaccttcag cttcccgcaa
    13021 gtggcatctt tcggattgac ggaagcttcc gctgatgcga gaaagattaa aatcaaagcg
    13081 ggaaaatcca ccttcctcgc aaacggaaaa gcactcggta tgaatgaaaa tgaaggctgg
    13141 attaaattga tttgcgatgc gaccgatcat cgaattctgg gtgcgacgat gatcggtgcg
    13201 gaagtgtgcg aattgttacc cgaattaacc cttgcggcga caaaacaatt gacagcagaa
    13261 gatattctta tgaatatcca tgctcatccc actttaagcg aaggaatcgc ggaggctgcc
    13321 gggaatttgc tgatatgatt aagaaaggga ttgactgagt tttagaaaga ggaacaataa
    13381 atgaggcctg aaatatcagt tttatcgatt attttgatta tcgaagcggt ttggtggctg
    13441 gtttggattt tattggctct gatcagaaaa aagttttctg ttttcgccgc tcttttggga
    13501 acattattga tttggggact gtccgggctt ttcttggtca gttctgcccc cgaaggaggg
    13561 cctatgaccg gccaattggt cggattggtg attttagcca ttattccgtt tatcatgctg
    13621 ccgccgatgg tggtgatcat aaacgaatat cagcgaggtg tccttttccg gctcggtcgg
    13681 ttaatggcaa cccttgaacc cggattgaat atcattttcc cgtttggaat cgatcgggtt
    13741 gtgaaaatag acttgcgaac gtttacgatt gacgtctcaa aacaggaggt aattaccaag
    13801 gacaacgtgc cggtattggt ggatgccgta gtctatttca acgtatttga tcccatttta
    13861 gccgttacga aagttgccaa ttatactttg agcaccactt tgctcggtca gacctcctta
    13921 cggtcggtat tggggcaaca tgaactggat gaaatccttt ccaaacgaac cgaactcaat
    13981 gaagttttgc gaaaactgtt ggacgaggat accgatccat ggggaatcaa gatttctgct
    14041 gttgagatta aaagtattga attgccggat accatgaaac gggcaatggc aaaacaagcg
    14101 gaagcggagc gcgaacgccg ggcaaaaatt attgcagccg atggcgaatt tcaggcggct
    14161 caaaagcttc tggacgcggc ttccgtgatc tcaaaggatc cggcgacctt gcaactgcgc
    14221 tacttgcaaa ctctcagtga aatcgcggtt gaaaggaatt caacaattct gttcccgctc
    14281 ccgatggaat tgttaaaggc gttcggtaat aaacaagctc ccgaataagc aaagcgcaaa
    14341 agatttaatc tcgaagaata acaaagatcc ggaaaaattt ccggatcttt gttaaatcat
    14401 ttgctcccgt tcgattttat ttggaaacgt taaaacggat attcaaaata tcaccatctt
    14461 ttacgatata gtcttttccc tccaacctca gtttcccttt ggcttttgcc tcgttcatgc
    14521 cgcctaattc aattaaatca tcgtatgcaa tgacctcagc ccgaataaag cctttttgca
    14581 gatccgtatg aatcacacct gcggcttccg gcgccgtagc tccgcggtgt accgtccacg
    14641 ctcggcactc atccgctccg gcggtaaaaa aagacaattg ccccaataat tcataggaaa
    14701 gcctgatcac ccgattcagt cccaattcgg aaatatgata atcttccata aacatcgcgg
    14761 catcctcagg ggaaagctgg gctaattcca tttcgatttg tccccgaatc gattcgactt
    14821 tgctctgttt ataaggataa tcaatccgcg gaacttcctg ggtttcatcc aaattcaaaa
    14881 gaatcaatac cggttttctt gacagtaatc cgaaaccgga aatcgctttt tcttcttcgg
    14941 cggtcagatc caattcccgt aaaggctttt cctcggccag gcaggcatgt aatttttcga
    15001 ataattcaat ctctcgatcg atgactgctt tttccctgcc gccgcctttt cttttctcat
    15061 ccgtcaatcg ttccagtttc ttttcgacgg aaatcagatc gtttaataaa aaatccccgt
    15121 ccaatgtctg aatatctcgt atcggatcaa tacttccggc gggatgaggt acctgatcat
    15181 tggtaaaaca acgtacgaca tgaataaatc cgtccatttg tgccaatttg tttaacaatt
    15241 caccggatat tccgctcttg gcggagcttc cgtccaatcc ggcgatatcc gcatagttca
    15301 ctttggcata aattgtcttt ttcggatgaa acatctttgc cagtacgtcg actctctcat
    15361 cggggacatc aacgaccgca tcatgaattt ctattttccc cgtagcggtt cctaccggaa
    15421 aactgccccg ggttaatgca ttaaataaag tggtttttcc actttgaggc agaccgacaa
    15481 ttccaagtcg catgtaagat ctccttgacc acagttaatt ttgtgggtag aattgaagaa
    15541 cttcagccga agtggcggaa ctggcagacg cgcacgactc aaaatcgtgt accttgtgta
    15601 tgtgggttcg attcccacct tcggcacaaa aattataaca taccctccgt cgaaaaattc
    15661 gacggagggt ttttgttccg aaccccttac gataatatgc taaaattgag ccaacggatg
    15721 ttaactgtcg aaataaaagt cggtacagaa taaagtcgag atcacacaac ccgaaatgct
    15781 ctccgcttga acgaatttta tattgcggat tttcggattt tcaatcggat tgattcatat
    15841 ttcccaaaag acgttccctg ttttttccga ttggtatgta ccgaatatca ggctcttctt
    15901 taacattcga attacgaagt gaatatgcta tacttttatc agtacataaa ttgcagattc
    15961 gttcgatcga gaggagaaaa actatgaatc catcaaggtt tggactgatt ctcatcattg
    16021 ctaccctgct gatttatgga ctctgcttat ttctgagaaa taagataaag ttgctccctc
    16081 tttacaagaa tgattcaaat tctaaaaaac ttcagctcag tctttctcgc agtatagaag
    16141 aaggaaagcc gattcatttg gatgttctca atcgagacga atcacaattg atcgccccgt
    16201 ccgctttggt tgccataaat acctcggaac aacttgcaaa ccgttttgcc gcatccgatg
    16261 aaccgccgtt cgtaaccaat aattccggct tggttcatgc gcttacaaaa gatgcggtaa
    16321 aaatgggata tcgacaagcg gatctcgaaa atgaattttc cgcggcttcc ctcgattttg
    16381 ccggatttga ttctctttcc catcaaacaa cgatgcttgg tcttctggaa gagacaccct
    16441 atgctatgca cttgaacatc ggatccggag gtccggaaat tgcattgcaa gatatgatgt
    16501 ttcaaaacga agaagaaatt tatatcgccg gagacagttt actcggtcag gcaaccgggc
    16561 tggcaacagc ggatgacgta ttcatcgggg aacagctata cgaactcccc gtcataacac
    16621 agcctgaaag gaaagaagct gctccggacg tcggactgtt ggtgatggat atcctgcgat
    16681 atggctttat tgtcgcactc ggaatcggcg ctttaatgat cttgttggta taaaaggcat
    16741 taattcatgg atttatcttc aaaaaacaaa cggttaacgg ttcttgcagg gctcatcggt
    16801 attttctttc tgatttccgc ctattctgac cagccggctg tccgaaccgc tcgaaccatg
    16861 ctgattcatt gggcaagttt ggtggcggcc gttctgcttt ttatcgcaat tatcgatttc
    16921 gttatcgctc gcattcgcgg aatcaaagac catacaggat ctttcatcta caatttgatc
    16981 agtttattga tcttcttgat tgtcttcttt tacggctttt cgaatcaggc cggcgcatct
    17041 tccgttcagt cgactgtttt tatgattcaa tcctcggttg aatccgttat gaccggcctt
    17101 atttgtttgg taatgattta cggaatttat cgggtagcgc gatcacaaag aagcttatta
    17161 aagagttctt ttttattcag tacaatcttt ttccttgttt tatatagcgg attcttttct
    17221 tttttgaaat taccggcgtt cttgtcaaaa ataatagatt ttattcgaat attgcccgtc
    17281 ggcgggcttt acggtttact gatcggtatt gccatcggtg cactggtgac gggaattcgg
    17341 gtattgtttt tcggtgatca cccctatcaa gggcataaat catgagaact ttcaaatgtc
    17401 cgatctgcgg ttcatcaaac gaggaaaatg cgaaaacctg cgcagtctgc ctggttaact
    17461 tcagtcaatt gcccgaagat ctccaattgc aaattcaaca cgaattggag aatcaacagg
    17521 aagccgttcc gcttcaggaa gatgaaaaaa atgaccggca aaatgaagtg ccggactggc
    17581 ttcggagacg gattcgaccc caatcggatg aaaaagaaaa taaatccttt gataattatt
    17641 tggatttaat ttttcataaa agcgtagagg aaaaccaatc gtccgcggat ttaaacgaga
    17701 aaagcgaagg ggattctttt ccgttcaccc aagaagattt cgaagcgctg gaaatcaaag
    17761 aagacggtgt accgcaagag acagaacttt cacccgaaat cgaggaatca ctctcggact
    17821 tttcctccgt tcgtcccgcc caaaaatggg atgaggcatc atccgataac gaaattgtcg
    17881 acggcaaaaa aatcgtatct tccgattccg aaaatgaatt cgttgatttt tcaaaaagca
    17941 gacccgctaa aaagtgggag gatttgacgc cggttccgaa cgaaaaaatc ggtatctccg
    18001 ggaagagaga aacgacgcat ccggaatcgt ttgaatctgc aaaattcgtt tcggaacaaa
    18061 aaagggacaa gacgcctctg gccgatcaaa agaacgaaaa tatcaaaatt aattctctgc
    18121 cggaggaatc ttttgtcgat ttttccgtag tccgtccgca acaaaaatgg gattcgggac
    18181 aggaaagtga tgccccttcc gtattcagtg ttgatgaatc gagtcatgat gtttttcggg
    18241 atgaacaatt aacttttttt gagaataaat ccgccgagcc acaggacgta aattccgaat
    18301 tgacagagga agaagccctt tatgccgatt tctcggttaa tcgccccgaa aaaaaatggg
    18361 acgacggaaa cgaacttaat tctcctacgg ctgattcttc gtcctcgctt gaaacaacgg
    18421 aaacagtttc aaacaagcaa ccgttttcgg tgacggagaa tgagaactcc gccggcaaaa
    18481 atgcggtttc tgaggaagcc ggtctggtgg attcattttt aagacaaata gctgccggta
    18541 cggaaacgcc gcaggaaccc attcaagaat ccgctgcgat gacttcgccc gaatgggtta
    18601 caccgcccga accggctccg cagccgtctt ccggcataga tgaagagttg gaatttctaa
    18661 tggagaaccc ggcgccgtcc gccgcagaag tccgagaaga agcggtaacc ggacagaatc
    18721 aagaagaacc atccgtctcc gaatccgttc cggaagagcc tgagccaccc gtttcctctg
    18781 aagggtggac acaggacgaa atcgttcccg atgaattgga atttgaagaa gacgatttgg
    18841 atgaggttcc gtggaattta tttgaaaccg gtgaaatgtc tttacccggg cttgaaccgg
    18901 ccgagatagc ctacaaacct ttctccaaat cgggcataca agaaggaaaa aacaatcaag
    18961 attatcaaca aagaatggtt atgaccattc tggataagat cttccaaatc gaaggtttaa
    19021 ataaaccgtt cgttaaaaat caaaaccgcg gaactcggaa aaatacacaa tttttgatcg
    19081 gattggtcat gattctgggt attcttgcca ttttgtttac cggaataacc gattccatcc
    19141 cattgaatcc gcccgatccg gagtctttcc ccaatttggt gacttttaat cgggaggtgg
    19201 acaaactgac aaacaatgat catgttttgg tcgtcgtcga ttacattccc ggctttgaaa
    19261 acgagatgaa cgaaccggtt accgcactgt tgaaaaatat caccgataaa aaggcgcgga
    19321 tcagcctcgt gacgaccaat ctttcttcgg cggctgtcac ggcaaagctg aaagcggaaa
    19381 tcgatccgac tgggatcgag gatatcggct accagaccgg cggtgttttg gcgatccaat
    19441 ccgtcctgca aaatgatctc tctccttaca caaccgttta tttggtcagt tccggatttg
    19501 aatcggcaag aacctggctg gagcaaatcg cttcgatgtc cgttccgttg cccgtcaatg
    19561 tcatcgcgtc gacgcagttg gaatctttat tgcagcccta ttacgaaagc aagttgatca
    19621 acagttatct gaccgggaag atggagaagg aaattttcag cggcttcact ttcgatgatc
    19681 cgatccgaaa tcgcagatta ttcgctcttt ggtttttaat tttcacttta atcctggcat
    19741 tcctcgcggg aaatgttcaa cgaacaccgt atcatacaat cattgtcgaa gaaaaaccaa
    19801 aatcgcgacc gccgaaaccg tcgacatcgg acaaaacaga aaacaaaatc gataaaggaa
    19861 tcaaaaatga catcgccttc tgatttcacc gttctgctca tcggattcct tttatccgtt
    19921 tttactttca gcctgatact gggtgacaac tggttgtttc gcctttcttc cgccgtattg
    19981 tccggggcgt tggcaggcta tctgattaac gtattgatcg aaaaaatttt cattccatcg
    20041 atcgtaaatc cattaatgga gtcgaacgtc gattggaaaa accaagcgct ggccgtcgtt
    20101 gtaatcatat ctgccgtatt tctgtttttg aagtattatt tccgctcccc cgcgggcgga
    20161 aatgcgattt tggcagtttt gttatgcagc gcggctgcca taaccattct cggaattgtt
    20221 aacggaactc ttatcaatct ttatcgcggc ttgatcgctc gttttcttcc cggtgcagaa
    20281 atgagttccg ccaatatata ctgggtaaaa acagggataa ttactttggg tgtcatttct
    20341 tcgttaattt atacccaaca ctatgctttc ggcaaaaaaa acggaaagga aatcagtcga
    20401 caaaggaata ctgttttctt tttcagaacc ctgggagaag tttttatcgg aatagcgatg
    20461 ggtgccgttt tcgcaggctg ttttatcgcc tcatcaacaa ttttgatcgg tcaaatttca
    20521 aatctcgtcg gatccggtca ggaaatactg caatgggtga aataagatga accggaatca
    20581 ggatattcat accgtgttat atgtcgatat cggttccgtc aacacgcgtg tttgcttatt
    20641 tgatcggatg gatgatcaat atagttttat cggggcggcg tccgctacga cttccggaga
    20701 tataacaaat cagtttgaat atctgaacgt gatccaagcg gttgaaaagc tggaacatgc
    20761 caccaaacga aagattttgg accgtaatca gaatattgtt atgccggcta ctctcgacca
    20821 ttctggcatt gatcaagtcg cggtttctta tacgtgcatt cccgatccgc gtatcgctgt
    20881 tatgggtctc acacatgcgg gaagcctgca aaatgtttat gatttgcttt cgaaagtcgg
    20941 agtgaagccg gttgcggaaa tttgtgccga agacggtaaa accgtttgtg aaaacttaga
    21001 cctgttcttg cataccgcac ctcggattgt cattgtcagc ggcggaacgg aacaaggcgc
    21061 agaaatcggt atataccgct tgggcgaaat cctgatgctg gcatgcagaa gtttgccgaa
    21121 agaacaacgc ccgatcatcc tctatgcggg gaattcggct gcgaaaaaac aaatggaacg
    21181 ggtcttttca cagatcaccg atatctcatt ttcgaccaat gtactggaat ccggctccgg
    21241 tgttcaaccg acgcttaacg gcttgatcaa tgtattgaga aaagatttgg aatcacgtgc
    21301 gcccggtttt cggattctac gggaaaaaac caagtgctcc gtcattccgg gagaattcgc
    21361 tttcggacga acgatccgtt taatgagccg gttgattcgt caaaaccggc acatcttagg
    21421 aattgatatc ggtgcgcatc aaacgatttt ggccgatgcg gttaacctgg ctctctcgtt
    21481 acacactctt ccgctgggca tcgggaataa catcggaaat ctcttgaacg aatatacacc
    21541 gtccgatttg caatcgtgga tcagttttca aatcaaaagc gccgagattc aggattatat
    21601 cctgaataaa agttattatc ccgatctgat ccccgccaac aaatattcgg cggaaatcga
    21661 actggcttta gcccgcgcaa ttgttatgaa gggtattgag aatgaatccc tgagcgccat
    21721 gctttctgac ggatcgttgg cgatggtatt tctcagcggc gcagtcttac gcaatgtcga
    21781 agatcccgga gaggcattaa tgtttgctat ggatagcttg gcacccgtag gaatggtcga
    21841 ttatctgctt gatatgaacg ggctatcggg tgcgatcggt acgctggcgc acttgaatcc
    21901 tgtgttagct tcccaaatca tgagcacatc cgcctacctc aatcttggaa aagtcattcg
    21961 tccttccgta cggggtgaac cgccgggcaa atccataatg actgtttccg tccgtgatga
    22021 tgccggaaat acacgaaaat atgacattcc ctttaatcga atttatcgga ttccgctgga
    22081 atacggaaaa aactatgaat tggaatggac aaaaatatca cataacgtca ctgttccggg
    22141 tgttaagacc tgggctcccg ccggttttaa aagcggctgt ttcgggttgg ttttcgatac
    22201 tcgggaagaa aaagacggga aaattaaact gccgaaagag aaagccgccc gtgacaagaa
    22261 actccaatcg tggcggaccg aactgggtcc ctggctggtt aaacaggagg aagaaagcca
    22321 tgcctgagga agcggttcat ataatctcat ccggtacggt gcgctgtgcc cgattgcttt
    22381 ccgaagacgg caccgttgtg gtattcaagg ggcaaatggc cgatccgaca acccaaatcg
    22441 ccttcagaaa aatttggagc cgacattttt ctctcaatat tctggatgca ttggatttgg
    22501 atccggcgga taacattcgt gagtttttga cgattaagga aggggatttt gtatccgtcg
    22561 gagaacaaat cgcgaaacgg gaaggcagaa ggaaattatc cgtcacagcg aaggcatccg
    22621 gtcgtttttt gggaatttcc gcgaatcgat tgatctttga aagcgatccc gttgacatcg
    22681 aatcgcgggt tgcgggattc ccgggtgtgg tgacggatat cgtcaataat cggggagctt
    22741 accttgaaac aagcggaagt tatattaagg gtttgtgggg aaacgggaaa tgcggacagg
    22801 gcattcttct ttccatcgat atggaaaaaa ataacggcat ttttgatttg gacagcatct
    22861 ccgtggacct tgccggagct gtcgtttttg cgaatacctg caccgatgcg aatgttttaa
    22921 aaactgccgc taaaatgacc cccggcggac tgattttcgg atcgttaccg tcctatttgt
    22981 taccggttgc ggtaagcctg cctttcccga ttatcgtaac ggattggatc ggaaccggga
    23041 aaataagcaa ccccgtccac ctcacgctgg gagaaaatat cattaaattt gcttatttat
    23101 attcggacaa cagcgcctcg ggaaagttga ttcgaccgga aatcatcata ccgcaggatg
    23161 aatatattcc ggaaaaggaa aacccgctca atcatttggg aatcggatcg caagttcgaa
    23221 ttatcgaggg cttttatggc ggagagttgg gaatcgtggt tgacattctg ccgggaaacg
    23281 atgagaattc agataatacg gaaatcgcac cggatcaggt gaaaatctgc attgatgaag
    23341 agacatatgt tactttgccg aaaaacaatg tcgaagtaat caatttgaaa agttagaaaa
    23401 ggggatcctc atgctggatt tcgacgataa ggaattattg gaaggctttg aagaagagtt
    23461 caaaaagaag aaacgcaaca agcgatcggg gaacagattc ccgtcatgga tcattatcgc
    23521 tataatcctt gtcgccggtg ttttcttctt tttatataaa aaaccggaca ccgtgaaggt
    23581 ccaaacaaac aaaaccccga cagttgaatt aacagaaggt caacaatccc ttattcagac
    23641 tcggcagata agcagctcaa ccgtttt
//
LOCUS       DAOSMM010000031        23361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2203_length_23361_cov_18.443834, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000031 DAOSMM010000000
VERSION     DAOSMM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 23361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23361
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_23361_cov_18.443834"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..683
                     /locus_tag="PK898_05975"
     CDS             15..683
                     /locus_tag="PK898_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU19618.1"
                     /translation="MVERISLFLPFYLGHVNCYLIKTADGFVLIDTGGTNQRQKLLRQ
                     LASCQCSPGKLRLIILTHGDFDHSGNAAYLREHFGGRIAMSPLDQGMIEHRDLTYNRK
                     GTPHFTKPFLPWIYVFGKSDQFIPDLLLYDGTDLTSEGFSATVYSIPGHTKGSVAVLT
                     IGGDLFCGDLLVCRNRVVMNTLLDDREDAIASIRKLRNLPAHMVYPGHGKPFPMDVFT
                     KGRF"
     gene            complement(774..1670)
                     /locus_tag="PK898_05980"
     CDS             complement(774..1670)
                     /locus_tag="PK898_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU19619.1"
                     /translation="MQTQSQLTIVIPAFNEEKSLQTFLPQVLNYCDENHFRLIVVNDA
                     STDRTAEVLDHFQNQFPEIMKIVNHKVNKGYGGALSTGLKQAETPFVVTLDADGQHRL
                     EDVTALMQEQQRTNADLVIGRRPEKINGDLQQSYRSLGKKLIRRVAKMMMDVPVRDLN
                     SGLKLYNTELVQKYLPLCPESMAFSEIITLIFLQNKHLVTEIPVEVVPRKEGKSSVTT
                     MTALETIIQIFNIIMLFSPLRIFLPVGTFLMAVGILWAIPFLIHGNGLSTTALLFITT
                     GLILVMMGLIAEQLAQIRRKDF"
     gene            complement(1758..1841)
                     /locus_tag="PK898_05985"
     tRNA            complement(1758..1841)
                     /locus_tag="PK898_05985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:Leu,seq:gag)
     gene            2015..3013
                     /gene="plsX"
                     /locus_tag="PK898_05990"
     CDS             2015..3013
                     /gene="plsX"
                     /locus_tag="PK898_05990"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOU19620.1"
                     /translation="MAIVLDAMGSDDCPQPEIEAAIYAAKNLNESIILVGHKDIIREH
                     VSESDFAGLPIEIVHAEDILEMGEKAVKSAQLKPNNSMAVGLRLVKEGKAEAFLTAGN
                     TGAAMFNSLRELGRIRNVARPALTALFPTAKGFSVVIDIGANSDCRPEYLLQFAVMGS
                     IYAEAMQKISKPKVGLLNNGEEPGKGNELMRTTYPLLESSKKINFIGNVEAKEVFAGK
                     ADVVVTDGFTGNIFLKTSEATAKMLTDILKEQLMPSIASGGDNSLAKNILSAVKSKMD
                     PNAVGAAPLLGIDGLVFVGHGRSNSTALISAIKVVREALNVDLVGTVRQSIQEELA"
     gene            3034..3735
                     /locus_tag="PK898_05995"
     CDS             3034..3735
                     /locus_tag="PK898_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19621.1"
                     /translation="MYIYTNERKLNSNRKKSKYITAISFAILIAGAVAAFSPQYFTWS
                     FIALIAGVILSQISISMTTRWGREPSNNNIFNVKLKGLSDNFSIYHYMDPVDHLLVGT
                     AGAFILMPYFQGGVIGYDEKKQRWTQKKASMFMKIFGQESLGRPDLDAEANLEAVKKY
                     FISRGIDFPEDKIHPILVFLGPKSSIDPNANFKYDTIMLDKLKDTIRKYAKEDRLSPE
                     FIDEVTDKLPLDDIE"
     gene            complement(3864..4256)
                     /locus_tag="PK898_06000"
     CDS             complement(3864..4256)
                     /locus_tag="PK898_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOU19622.1"
                     /translation="MDDLYRENILDHYKNPIYKGTLEPADLSFEDENPLCGDHLRIDL
                     RVDEKGNVTDARFSGHGCAISQASADLLLESIIGKNIEDLKKLTKEDILDLLGIEELG
                     PVRLKCALLSLKVLKGSLYGIGQKDEDL"
     gene            complement(4258..5487)
                     /locus_tag="PK898_06005"
     CDS             complement(4258..5487)
                     /locus_tag="PK898_06005"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOU19623.1"
                     /translation="MDEKQIIQDFPIFSQTDRNGKTLCYLDSAATTQKPLSVLEAMDT
                     YYKTYNANIHRGVYHISELATEAHETTREKVRAWIHARSTTEIIFTRNTTESINLVAY
                     SWGRKNLNAGDHIILTEMEHHSNLVPWQILAEEKGVELDFIPITDAGELDLEVYRQLL
                     EKHPKLVCATGMSNVMGTIPPSKEMISAAHAAGALFLLDGAQMVPHMQVNIRDLDADF
                     FAFSAHKMLGPTGIGVLYGKESLLEVMPPFLGGGDMIKKVHLRSFSANDLPYKFEAGT
                     PAIAEAIGFGAAIDYLAQIGMDAVFEHESEITRYAIHELMKIPGLKIIGPLTSSNRGS
                     AVSFTLDYVHPHDVAQILDRENICVRAGHHCAMPLHERCNVDASTRASFYIYNSLTDV
                     DRLIDGLKIVHQLFGQD"
     gene            complement(5510..5776)
                     /locus_tag="PK898_06010"
     CDS             complement(5510..5776)
                     /locus_tag="PK898_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19624.1"
                     /translation="MGAIPTMREPIPEVNYEVGDLVEVLCDHDRKGVRVNGWLKGIVV
                     QVDNKMIAVQFRTNVYLTDGWMVPDHILWYPLNSPNIRPYQKKA"
     gene            complement(5821..7050)
                     /gene="sufD"
                     /locus_tag="PK898_06015"
     CDS             complement(5821..7050)
                     /gene="sufD"
                     /locus_tag="PK898_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOU19625.1"
                     /translation="MISNNQQNLSVQSNYEYLFKLIQSADGSNLDRIKTQTAEMLAEN
                     EYTTAEGSPQTLTDANERVLAGRFLWEDGVRSTGLDSNTRKQGVLLDTLENIRANQPE
                     IFLKIKYWQESLQQNPLEALIGSIAKETIVLYVPKFAVVSKELLLDVVLSQPGRNALM
                     RFWVFLEDGAQAVFTINLRSRKAEGENFYTGCLQVRVGDGAHLILNEVQDFHQKVYVT
                     ATKSVTTGKVSNIQWNTCELGSIKLRNNATMNLKGEGSNGLIYGLYFATDHQDIEMQT
                     RQNHLAPNTTSFLHFKGAINDDAYANWQGMIYVDPAAKKTDGYQKNENLMLSPNARVF
                     TKPGLEIVTDDVKCSHGTTVSEIDADQIFYLMSRGMSKNESELLILQGYFDAILNNIT
                     YDPIREKLKEKIYLKMI"
     gene            complement(7058..8473)
                     /gene="sufB"
                     /locus_tag="PK898_06020"
     CDS             complement(7058..8473)
                     /gene="sufB"
                     /locus_tag="PK898_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOU19626.1"
                     /translation="MDNPQKYQLQGIDDADYEYGFQDPDISVFRTEAGLNEKVVRQIS
                     AMKEEPEWMLEFRLKSLKHFLERPMPAWGPDLSELDLDHLVYYVRPTEKPQNNWDEVP
                     STIKNTFNKLGIPEAEQKFLAGVGAQYESEMVYHNLQEHLSKQGVIFLSIEDGLKQYP
                     DLFRKYFGTVVPYNDNKFAALNSAVWSGGSFVYIPKGVHVDLPLQAYFRLNSANIGQF
                     ERSLIIADEGSQVHYVEGCTAPQYSTNSFHSGVIEIIVGRNARVRYSTIQNWSTNVYN
                     LVTQRAKVFAHGTMEWVDSNLGSKVTMKYPACYLMEDGAHGEILSMAFAGKGQIQDAG
                     SRMIHLAPNTTSKVTSKSISKNGGRSSYRGYMKINKNAENAKSNVVCDALILDDHSQS
                     DTYPTMDIDQQKVSVGHEASVSKIGEEQLFYLMSRGLSEEDATTMVVSGFIEPLVKEL
                     PMEYAVEMNRLIQLQMVDSIG"
     gene            complement(8496..9266)
                     /gene="sufC"
                     /locus_tag="PK898_06025"
     CDS             complement(8496..9266)
                     /gene="sufC"
                     /locus_tag="PK898_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOU19627.1"
                     /translation="MIGLEVKDLSVTVHDKKIINHLSIQVPKGEIHAIMGPNGTGKST
                     LSYTIMGHPAYRPDEGEILLNDQLINDWPPDKRSHAGLFLALQYPVAIPGVTVANFLR
                     TALNIHRKSKNPDDHGIPIPEFRKLLKEKMKMLKMDPDFAGRYLNDGFSGGEKKRTEV
                     LQMAILEPEIAILDEIDSGLDIDALRIVSEGINILVDQTHMGVLIITHYQRLLDYIKP
                     QAVHVMMDGQIVRTGDANLALELEEKGYDWIRQEQTTA"
     gene            9597..10943
                     /locus_tag="PK898_06030"
     CDS             9597..10943
                     /locus_tag="PK898_06030"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOU19628.1"
                     /translation="MRIIFLRHGEAIERGSVDDEQRFLTERGKRDLESNLPALAAYLR
                     NRNNVEIWSSPKIRAIETAQILTSGMENKDIRFKDFIGDGDTEQLKSELEKTGAETVV
                     IVGHQPYLSEWVDELCDSEILFQKGAAAEIEYYPDSDQKGKVLWHFPLKGLKQLARMM
                     GPEVEGNSFRQDLMIKMEQNFNQIAENRNRFLSEPDDVESAHQLRVSIRSFRSLISLG
                     KKYLKPEKYQDYQDQFRQAAAEISTLREIDVIINTWNEHLLDQKLDPSQSVLIQELNR
                     KRNIEKDRVIKIVSKNKYIDRLTKSSDDLIKTIRKSSEQDVPTQKVIDARLDKWYQYI
                     LKRMINYNEFNFSYIHPIRLKCKKYRYVSENFYGILDPEHRKRHRDVKQLQTLLGNTC
                     DAIRNQEAILKIIGLRSPALEQEVIAFINYEKEVESNYRKLFDSREWLQQISEKEN"
     gene            11070..11897
                     /locus_tag="PK898_06035"
     CDS             11070..11897
                     /locus_tag="PK898_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU19629.1"
                     /translation="MNEIFEKFSMKDQTAVITGGGGLLGKEFCKTLAQAGATIVAADL
                     NLNLARQTVELIQEFGGHGIACAVDVTDPESVKAMVDLAVNETGRLDVLVCSAALDPK
                     FDGTQGKHSNTFEEYPLKQWRDALDVNLTGLFLCAQAAVKPMIAQNHGVIINICSTYG
                     LVGPDQRIYERPDGDPSSRQYKPVYYSVTKAGVLGLTKYLATYYAGTGIRANCLTPGG
                     VYNGHDEFFVKQYSARTVMGRMANIDELSGALLFLASDASSYMTGSNLVVDGGWTAW"
     gene            11891..13144
                     /locus_tag="PK898_06040"
     CDS             11891..13144
                     /locus_tag="PK898_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HOU19630.1"
                     /translation="MVAKHAEVLAIIPARGGSKGIPHKNIKKFAGYPLIAWSIAAAKQ
                     SASVTRTIVSTDDEEIARVAREWGAETPFLRPAEFAMDQSLDLPVFRHALEWLKENEG
                     YEPDIVVQLRPTSPVRPVNLVDDAVRLLTDHPEADSVRGVVPSSENPFKMWLIDSETG
                     TMSGLIKLDGIPEPYNAPRQVLPATYWQTGHIDAIRPRRTFMAGDSMSGKVILPLLLD
                     PAFKVDIDTLADWDRYEQLVYYGGLDMVYPEHKRRSLPQKTCLLVMDFDGVLTNDEVI
                     VNQDGLESVICNRGDGIGFWLLREAGITAIVISSETNPVVRKRCEKLGIQSYQGTLDK
                     ATVLKKYLEENKIPAENTVYIGNDINDLQCFPLVGCAAAPADAHDLVKSKADIILKRN
                     GGQGVVRELCELIVRRNKESYGKDS"
     gene            13128..14003
                     /locus_tag="PK898_06045"
     CDS             13128..14003
                     /locus_tag="PK898_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOU19631.1"
                     /translation="MGKTVKLGNRLVGEGQPVYVIAEIGINHNGDIGIAKRMIRSAYA
                     AGVDAVKFQKRTPAVCVPLSEQSKMRETPWGYMSYLEYREHVEFWEDEYHEIDELCHE
                     LGIDWFASVWDVESIDFLMKFDPIAYKVPSASLTDHELLDKLLSTRKPIILSTGMSTM
                     DQIRQSVSLFGNLDNLIITHATSAYPCKPEELNLRMIPKLIEEFDCPIGYSGHEVGLI
                     PSVISVALGACLVERHFTLDRAMWGTDQAASVEPQGMEKLVKYIRVTEASLGDGIKKV
                     YDDELPSLKKLRRVQ"
     gene            14014..15693
                     /locus_tag="PK898_06050"
     CDS             14014..15693
                     /locus_tag="PK898_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19632.1"
                     /translation="MAVDSSKRSVFMRIRDRRRIHDTDGANSRKFHEINRYSSQRVRK
                     DEQPVLFFNASTRLTRTSLNAAFAWLAANSLQSTGVPVVFMTCMRGLNPCLLATDREN
                     PKKELPCQSCIRLSKSMYNGLQTVPLEFHEDPILFNEVEGLTYHELESYVHEGVPLGE
                     LTLPSLRWIMRRHHLEDVPNALPLQRLYIRSAWSTYLLAKEWISNNHPRAIVVFNGLQ
                     YPEATVKWIGKQNGIPTFTHEIGLMPETAFFTEGEATACPIDLPADLEMTPERNKKLD
                     DYLESRMKGSFQTAGVRFWPEMEGLTPEFWKMVRMFRQVVPVFTNVVFDTSQKHANVV
                     FDNMFEWLDSIKSLIEKNADTLFVIRAHPDELRPGKESLETVTDWVVGNHLTHLTNVL
                     YIAPDQYFSSYDLIRIAKFVMVYNSTIGLEAAAMGAAVLSGGKSRYTQVPITYFPQSK
                     EAFIQKAQDMLDADEWVVPDEFQQNARKFMYYQFFHTALDFSEFLSPDPVWNGYVKLK
                     DFKPESLLPENNETMRVIQDGILRGKPFVYPHSFEKDPQLINSSVQMKNDK"
     gene            15706..16380
                     /locus_tag="PK898_06055"
     CDS             15706..16380
                     /locus_tag="PK898_06055"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOU19633.1"
                     /translation="MKIVAFVPMRHNSVRVEGKNYRMLAGKPLYHYIMEALLAVPEIN
                     EVVVDTDSPVVIEGLAKDFPTVKSLLRPEALRADDVPMNEILIYDTSQVQADYYLQTH
                     STNPLLKSETISKGIRSFLEKTYLYDSLFSVTRVQKRFWDQLARPINHNASILLRTQD
                     LPPIYEENSCMYLFTREILMRKRHRIGDRPMMFEIPAIEAQDIDDETEFNITEMLMKL
                     RQQGKL"
     gene            16507..17484
                     /locus_tag="PK898_06060"
     CDS             16507..17484
                     /locus_tag="PK898_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOU19634.1"
                     /translation="MKHTIILSAPYMMSSVERFRKVIEGYYGINMIVPEVKQKLTEDQ
                     IISYAGQFDGTICGDDVYSARVIEACVPRLKVISKWGTGIDAIDKEAATRLGVVVGNT
                     LNAFTIPVSETAIGYMLSFFRAIPFMDDSMREGIWNKPSTTTLSENTIGIVGVGNIGQ
                     AVVRRLRAFGCRILVNDIAEIKPDFLIENRIESVSLPELLSRSDIVTMHTDLNPTSYH
                     LINRETLAMMKPTAILINTSRGPIVDEAALAEALENGKLGGAALDVFEVEPLPDDSPL
                     RKFKNVLLAPHNSNYSPMACERVHWNTIRNCLIPLGYDVSKLEELKKTI"
     gene            17549..18361
                     /locus_tag="PK898_06065"
     CDS             17549..18361
                     /locus_tag="PK898_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOU19635.1"
                     /translation="MSTSKTNMKNSVPENILLKGKELYDGIKRLGDIPEAYFDPVRRD
                     SMERLSRLKDSRKGERCFIMGNGPSLKNTDLSKLKNEYTFGLNRIYLAFPEMGFETTY
                     YLCVNDLVVEQTSDDIQKLKMPRFVTTRALKWLKPEENLFFLYSTYTGPTFATDIRKR
                     MWEGATVTYMALQTAFYLGFRQVILIGVDHNFQTKGKPNETVVSEGDDPNHFNPGYFG
                     KGFRWQLPDLETSERAYTMAREAYQKAGREILDATIGGKLQVFPKVAYDSLF"
     gene            18368..19327
                     /locus_tag="PK898_06070"
     CDS             18368..19327
                     /locus_tag="PK898_06070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU19636.1"
                     /translation="MSHPHISVVTPSYNQAPFLEKTIQSVLTQKTPEIEYFVMDGGST
                     DGSIDIIRKYESEIDHWVSEKDRGQADGINKGFSLAGGEIFGWINSDDFYLKGAFEKV
                     LAYFDAHPEVDLVYGDVLSVNQEGELINVMRFADYTLRDLMAFRIISQPAVFFRRKAW
                     DGAGGMDLRYHFLLDHHLWLRIAARGKIAYLPEPLAAARFYPEAKNRAHAADFGAEAY
                     RIADWLLTDPEMKPYSKGYRRVIQGGADWLNANYLSNGNHPWKSLKNYAKAFTKYPSR
                     VFEDRRRVGVTLLMAISPEKATDVFRREAQKRMDAFSAYRDLL"
     gene            19324..20841
                     /locus_tag="PK898_06075"
     CDS             19324..20841
                     /locus_tag="PK898_06075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19637.1"
                     /translation="MNLHTIPSGKKLTGWAETVIVLILMAAGFAVRLYDLTDPPLDFY
                     SPRQLRSAIITRSFYYESLNNSDPTIVEQADKLADLERYEPPVMEWLVSKIDLLIGKE
                     VFWTGRIFSAFFWALGGLFLYRICRRFASFSAGIFSLLIYYFVPFSIIASRSFQPDPW
                     MCFWVLGTAWMLCNWSEKGSWSSAFFSGFIGGMALLVKIYAGFFVAFMLLGIFCASVN
                     RQNRKQRIFQTLLIGAVMLIPSAVYYLIFNPSRSGDFFSFWIVSLSGLIRTSNFYADW
                     IAMVKGLIGLVLPLFGLWGVLFIRNRQVKGLMIGWWLGYIFYGLFVPYQITTHEYYSL
                     ILIPLTAISVCPVSDTIFEKTRQSGKIAFVCSLLLILAAAGYGAYSACGYLRGTNYVL
                     EPASWRKVGEAIPQNEPFIALTGDYGMRLIYYGWRQPSLTWPDAKDDTLFQLAGTPQA
                     DFDTYFDSQTKGMNYFVVTALGELDNQPDLKKRLESFPVYAAGNGYIIYDLRNKP"
     gene            20838..22355
                     /locus_tag="PK898_06080"
     CDS             20838..22355
                     /locus_tag="PK898_06080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19638.1"
                     /translation="MRNIVARKKLSSIQIIVFILIFLCGTFLRFVDFDDPPLDFHPAR
                     QLHSALIARSYFLKDNGNLPGKSADYQHEAEMRGSQEPRIEPPIMERLAAWGYKIVGD
                     ADLRVPRAMSIVFWLIGGIGLFRLCRRFLGITGSLVGFGFFLLFPYTIFASRSFQPDP
                     LMVALMIWSLNALVEWDSHPAIGPAILTGVLTGTAILVKQVCVFPLASAFAFYIFSSS
                     DRKQKIKRVDFWIIVALSVAPVLIYNYWGYFVKGFLVQQYQGRFSLSDWFSPAFYVRW
                     IRKMDEVFGIVVVTAGLVGCLACENRRARLLWIGFFAGYILYGLALPHHIGTHDYYQL
                     PLFPLISFGIGRSAESIFEVVGRLKEGRTLAYVGITVCLILLGAWWSADSVISLNRRD
                     YRAWPERWQSLAAELDPYPGQINTIGIMDDYGAAMIYWGLRTPVIWEQNIEKLSEEEA
                     DTIIRQAMINREYLIVTDLDSFYAQPRLQNWLVRNTKLYAEEPDYLIYDLRGLNE"
     gene            22348..23133
                     /locus_tag="PK898_06085"
     CDS             22348..23133
                     /locus_tag="PK898_06085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU19639.1"
                     /translation="MSEQAVFPFVSIVTPSFNQAEFLERTIQSVLSQDYPNLEYIIID
                     GGSTDGSVEIIRKYENRLAYWVSEKDKGQTNAINKGFNRAKGEIFAWLNSDDIYEPGA
                     IQAAVEALMSDPSLGFVYGDCNFIDSHDRIIGKFDARQTDLKKLQRGYVHIPQQAFFF
                     RGELWKKVGPLDESIYFAMDYDLWLRLARIAPFRYIPGKVWADFRLHEDAKTITADAS
                     CWPEMIQIRRRDGGSKFSVLFLKYQLRKLMAPLINYRRRKMFR"
BASE COUNT         6316 a         4823 c         5593 g         6629 t
ORIGIN      
        1 gggaacggat atgaatggta gagcgtattt ctctcttttt gcccttttat ctcgggcatg
       61 tcaactgtta tctgatcaaa accgctgacg gatttgttct aattgataca ggcggaacga
      121 atcaacgtca aaagttgttg cggcaattag cttcctgcca atgttctccc gggaagttaa
      181 gactgattat cttaacgcac ggtgattttg atcattcggg aaatgcggct tatcttcgtg
      241 aacattttgg cggcagaatc gccatgagcc ctctggacca gggaatgatt gaacatcgcg
      301 atctaactta taaccggaaa ggaacgcccc attttacaaa gccgttcctg ccgtggattt
      361 atgttttcgg aaaatccgat caatttatac cggatcttct cctgtatgat ggaacggatt
      421 tgacatccga ggggttctcg gcaactgttt actcgattcc cggtcacaca aagggatctg
      481 tggcagtatt aacgattggc ggcgatttgt tttgcggaga tttattggtt tgccgaaatc
      541 gcgtggtgat gaacacgttg ttggatgatc gcgaagatgc gattgccagc atccgaaaat
      601 tgaggaattt gccggctcac atggtttatc ccgggcacgg caagcctttt ccgatggatg
      661 tttttacgaa gggacgtttc tgattgaccg ctacaaagga aaatcaatca accggtatat
      721 ttcagagaac agattcttga taaggatcaa gcgatctttt ctctgattct atttcaaaag
      781 tcttttctgc gaatttgggc taactgttct gcgatgagac ccatcatgac cagaatcaat
      841 ccggtcgtaa taaacagtaa tgcggttgtg ctcaatccgt ttccgtggat cagaaaggga
      901 atcgcccata aaattcccac cgccatcaga aatgttccga cgggcagaaa tattcgtaag
      961 ggactgaaca acataataat attaaaaatc tggatgattg tctcgagcgc ggtcatggtt
     1021 gtgactgaac ttttgccttc ttttcgcgga actacttcga cgggaatttc agtgaccaaa
     1081 tgtttatttt gcaagaagat cagagtgata atttccgaaa aagccatgct ttcggggcag
     1141 agcggaaggt atttttgaac cagttccgta ttatataatt taagcccgct gttcaggtct
     1201 cgtacgggaa catccatcat cattttggcg acccgccgga tcaatttttt ccccaatgag
     1261 cggtagctct gctgaagatc accgtttatc ttttccggac ggcgaccgat taccaaatcg
     1321 gcattggtgc gttgctgttc ctgcatcagg gcagtgacat cctccagacg atgctggccg
     1381 tcagcgtcca aagttacgac aaaaggtgtt tcagcctgct ttaagccggt agagagtgcg
     1441 ccgccgtatc ctttgttcac tttatgattt acaattttca tgatttccgg gaattgattc
     1501 tgaaaatgat ccaaaacttc tgcggtacgg tcggtagaag cgtcatttac tacgatcagc
     1561 cggaaatggt tttcgtcaca gtaattgagc acctgaggca gaaatgtttg gagagatttt
     1621 tcttcgttaa atgccggaat gacaatggtt agttgtgact gggtctgcat gaaatgatct
     1681 taatctaaaa tcgacgattt tacaataaaa aaagttatga ctcggtttaa aaaaatcgca
     1741 ttcataagcg atttttgtgc gctggggggg aatcgaaccc ccacgacgaa gatgtcacat
     1801 ggccctcaac catgcctgtc tgccaattcc agcaccagcg cgttagcttt tgtattataa
     1861 tcaggtacgc agcgttgtca aggaaatgag acgcaaactg atcttatcgg atcaggattt
     1921 cgctgtggga tgaatgatta agaataagtt gaaaatagga tgaaaaacct gtgattcggt
     1981 ttttttaaat agatttttac tgtcaggaga caatatggct attgttttgg atgcaatggg
     2041 cagcgatgat tgcccccaac cggagattga agccgcaatc tatgcggcga aaaatttgaa
     2101 tgagagcatt attctggtag gtcacaaaga tattattcgg gaacatgttt cggaaagtga
     2161 ctttgccgga ttaccgatcg aaatcgttca tgcggaagat atccttgaaa tgggtgaaaa
     2221 agcggtaaaa agtgctcaac tgaaaccgaa caattcaatg gctgtcggtc tgcgattggt
     2281 taaagaagga aaggcagaag cgtttttgac cgccggaaat acaggcgcgg ctatgtttaa
     2341 tagcctgcgt gaattgggac ggatcagaaa cgtcgcccgt ccggcattaa ccgcactgtt
     2401 tccgaccgca aaagggttta gcgtagtaat cgatatcgga gcgaattccg attgccgacc
     2461 ggaatatctg ctgcaattcg ctgttatggg ctcgatttat gcagaagcga tgcagaagat
     2521 ttccaagcct aaagtcggtc tcttaaataa cggcgaggaa cccgggaagg gtaacgaact
     2581 gatgcggacg acttatcctt tattggaatc ttcgaaaaaa atcaatttca tcggaaatgt
     2641 ggaagcgaaa gaagtttttg ccggaaaagc ggatgtggtg gtgactgacg gatttaccgg
     2701 taacatcttt ttgaagacct cggaagcgac tgcgaaaatg ttgactgata ttttgaaaga
     2761 acaattaatg ccttcgatag catccggtgg agataactct ttagccaaga atatcctatc
     2821 cgccgtaaaa tcaaagatgg atccgaatgc ggtgggggcc gcccctctgt tggggattga
     2881 cgggttggtt ttcgtcgggc atggccgttc aaacagtacg gcattgatca gtgctataaa
     2941 agttgtacgg gaagctttga atgttgattt ggtcgggacg gttcggcaat ccattcagga
     3001 agaattggca taaattaaac agaggaatga ttcatgtata tttacactaa tgaacggaaa
     3061 ctgaactcaa atcgaaaaaa atccaaatat ataaccgcta tttcttttgc gattttgatt
     3121 gccggtgcgg ttgcggcatt ttctccgcaa tatttcacct ggtcttttat cgctttgatc
     3181 gcgggtgtta ttctttcgca aatatccatt tctatgacaa cccgctgggg cagggaaccg
     3241 agcaataaca atattttcaa tgttaagctc aaaggactga gcgataattt ttcaatttat
     3301 cactatatgg atccggttga ccatttgctg gtcggaacag cgggcgcttt tattctgatg
     3361 ccctattttc aaggcggtgt gatcggctat gatgaaaaaa agcagcgttg gacacagaaa
     3421 aaagccagta tgttcatgaa aatattcggc caggaatctt tggggagacc cgatttggat
     3481 gccgaggcaa atctggaagc ggtgaaaaag tattttatca gtcgcgggat cgattttccc
     3541 gaagataaaa ttcatccgat tttagttttt ctcggaccga aatcatcgat cgatccaaat
     3601 gcaaatttca aatatgatac aattatgctc gataaactca aggatacaat ccgaaaatac
     3661 gccaaagaag atcgactgtc accggaattt atcgatgaag tgacggataa actgccgttg
     3721 gatgatatcg aataatactt caacgtaaaa ttcgaaaaga ttttgttgga aatctataaa
     3781 aatcgtgcgg aaatagcttc cgcacgattt attttataaa tattgatttg aaaacggagg
     3841 ggccggataa aagactttga tttctataaa tcctcgtctt tttgtccgat tccatacaaa
     3901 ctgcctttca aaacctttag agataataaa gcgcatttta atcggacagg ccctaattcc
     3961 tcaattccaa gtaaatccaa gatatcttct tttgtcagtt tcttgagatc ttcgatattt
     4021 ttcccgataa tggattcgag taataaatca gctgaagcct gtgaaatagc gcatccgtga
     4081 ccgctgaatc gggcatcggt tacatttcct ttttcatcca cccgcaaatc gattcgcaga
     4141 tgatccccgc acaaagggtt ttcatcttca aaactcagat ccgccggttc caacgtccct
     4201 ttataaatgg gattcttata atgatccaaa atattttcgc gatataaatc gtccatttca
     4261 gtcctgtcca aaaagctgat gtacgatttt caagccgtct attaaacgat cgacatcagt
     4321 aagcgaattg taaatataaa aactggcccg ggtcgaagcg tcaacattgc atcgttcatg
     4381 taacggcatg gcgcagtgat gtcccgcgcg gacacaaatg ttttctctgt ccaatatttg
     4441 agccacatca tgaggatgaa cataatctaa agtaaaactg actgctgatc cccgattgga
     4501 agaagttaaa ggtccgatga ttttcagccc cggaattttc atcaactcat gaatggcata
     4561 acgggttatt tccgattcat gctcaaagac cgcatccatg ccgatttgag ccaaataatc
     4621 aattgccgct ccaaacccga tcgcttcggc gatggcgggt gttcccgctt caaacttata
     4681 aggcaagtca tttgccgaaa aggatcgcaa atgaactttc tttatcatgt cacctcctcc
     4741 gaggaaaggc ggcatcacct ccaacagcga ctctttacca tataagacgc cgattccggt
     4801 cggtcccaac attttgtgcg ccgaaaaagc aaaaaagtct gcgtccaaat cccggatatt
     4861 cacctgcatg tgcggaacca tctgggcacc gtctaataga aaaagggcac ctgcggcgtg
     4921 agcggcggag atcatttcct tggagggtgg aatcgtcccc attacgttgg acataccggt
     4981 tgcgcagacc agtttcggat gcttttcaag gagttgccgg taaacttcga gatccaattc
     5041 ccccgcatcc gttatcggaa taaaatccag ttcgacgcct ttttcctctg ccaatatctg
     5101 ccagggaacc aggttggaat gatgctccat ttcggtcagg ataatatgat cgccggcatt
     5161 caaatttttt cttccccagc tataggctac caaattaatg gattcggtgg tatttcgcgt
     5221 gaatataatc tccgtcgtcg atcgggcatg aatccaagcc cgaacttttt cacgcgttgt
     5281 ttcatgagct tccgtcgcca actccgagat atggtaaacc ccccgatgaa tattggcatt
     5341 gtacgtctta tagtaagtat ccattgcttc gagaacggat aacggcttct gtgtcgtggc
     5401 tgccgaatcc agataacaaa gtgtttttcc gtttcgatcg gtttgactaa aaatcggaaa
     5461 gtcctgaata atttgttttt cgtccattct taatcctttt ctattaattt caggcttttt
     5521 tctgatatgg tcgtatattc ggcgaattca acggatacca aagaatatgg tccggcacca
     5581 tccatccgtc tgtcagataa acgtttgtcc ggaattgcac ggcaatcatt ttattgtcca
     5641 cttggacaac aatgcctttc aaccagccgt taactcggac accttttcga tcgtggtcac
     5701 ataagacttc gaccaaatcg cccacttcat aatttacttc gggaatcggc tcccgcattg
     5761 tcggtatcgc acccacttta acctccggaa tcgaacggaa aaggaatagt ccccgttcga
     5821 ttaaatcatt ttcaaatata ttttctcttt caatttctcc cgtatcggat cataggttat
     5881 attattgaga atagcgtcaa aatatccttg caggattaaa agttcggatt catttttgga
     5941 catgccgcgg ctcatcaaat aaaaaatctg atccgcatcg atctcactga ctgtcgtccc
     6001 gtgagaacat ttaacgtcgt cggtgacaat ctccagaccg ggtttcgtga agactcgggc
     6061 attgggagag agcatcaggt tttcattctt ttgatagcca tcggtttttt tagcggcggg
     6121 atcaacataa atcattccct gccaattggc ataagcatca tcattgatgg ctcccttgaa
     6181 atgcagaaaa ctggtagtat tcggcgccaa atgattctgg cgggtttgca tttcgatatc
     6241 ctgatgatcg gttgcgaaat ataacccata aataagtccg ttcgatcctt cccccttcag
     6301 attcatggtg gcgttgtttc gaagtttgat cgagcctaat tcacaggtat tccattgtat
     6361 attgctcact ttaccggttg taacggattt tgtggcggtt acgtaaacct tctgatgaaa
     6421 atcctgaact tcattcagaa ttaaatgggc tccgtcaccg accctgacct gtaagcagcc
     6481 ggtataaaaa ttttcaccct ccgctttacg cgaacgcaga ttgatggtaa ataccgcttg
     6541 tgctccgtct tccaggaaaa cccaaaaacg catcagtgcg tttcgaccgg gttgcgagag
     6601 aacgacgtcc aacagcaact ctttggatac aaccgcaaat ttggggacat acaagactat
     6661 ggtctctttg gcgatactgc cgatcaacgc ttcaagcgga ttctgctgaa gcgattcctg
     6721 ccagtattta attttcagga atatttccgg ctggttcgct cggatattct ctaaggtgtc
     6781 cagtaaaacg ccttgttttc gggtattact gtccaagcct gtgctgcgaa cgccgtcttc
     6841 ccaaagaaag cgtccggcaa gaactctttc attggcatct gtcagagttt gagggcttcc
     6901 ttcagccgtc gtatattcat tttcagccag catttccgcg gtttgtgttt tgatccgatc
     6961 gagattagag ccatccgccg attgaatcag tttgaacaaa tactcgtaat tcgattggac
     7021 tgagagattt tgttgattgt ttgatatcat ttttgcctca cccgatcgaa tctaccattt
     7081 gaagttgtat cagacgattc atttcaaccg catattccat gggaagttcc ttgacaagcg
     7141 gttcgataaa accggaaaca accatcgtgg tggcatcttc ttcacttagt ccgcgactca
     7201 tgagataaaa cagctgttct tccccgattt ttgaaaccga agcctcatgt ccgacggata
     7261 ctttctgctg atcgatatcc atggtcggat aggtatcgga ttgagagtga tcatccaaga
     7321 ttaaagcatc gcataccaca ttcgatttgg cattttcagc attcttgttg atcttcatat
     7381 aaccgcgata ggatgagcgt ccaccatttt tgctgattga tttcgaagtg accttgctgg
     7441 tggtattggg tgccaaatga atcattctcg atcccgcatc ctggatttgt cctttgccgg
     7501 caaatgccat ggaaagaatt tcaccgtgtg caccgtcttc cattagataa caggcgggat
     7561 atttcatggt cactttcgat cccagattgg aatccaccca ctccattgtc ccgtgtgcga
     7621 aaacttttgc ccgttgagtg actaagttat aaacatttgt cgaccaattt tggatggtgg
     7681 aataacggac acgggcattt ctgccgacga ttatctcaat cacaccgctg tgaaaggaat
     7741 ttgtggaata ttgcggagcc gtacacccct cgacatagtg aacttgcgaa ccctcatcgg
     7801 cgattattaa ggaacgttcg aactgaccga tattcgctga gttcagtcga aaataagctt
     7861 gaagaggcag gtcgacatgg acacctttcg ggatataaac aaatgatccg cctgaccaaa
     7921 cggcgctgtt taaagcggcg aatttattat cgttataagg gacaacggtc ccgaaatatt
     7981 ttctgaacaa atcgggatat tgtttcaaac cgtcttcgat actcaggaag ataacccctt
     8041 gtttggaaag atgttcctga agattatgat agaccatttc cgattcgtat tgtgcaccga
     8101 ctccagccag aaatttctgt tcggcttccg gaattcccag tttattaaag gtatttttga
     8161 tggtggaagg gacttcatcc caattatttt gcggtttttc ggtcggacgg acataataga
     8221 cgagatgatc caaatccaat tcggacaaat ccggacccca agccggcatc ggccgctcca
     8281 aaaaatgttt caaactttta agccggaatt ccaacatcca ctccggctct tccttcattg
     8341 cggaaatctg gcggacaact ttttcattta atccggcttc cgttcgaaag acggaaatat
     8401 ccggatcctg aaatccgtat tcgtaatcgg catcgtcgat tccctgtaac tgatattttt
     8461 gtggattatc cattctgttc tccggaaatc gatctttatg cggtggtttg ttcttgacga
     8521 atccagtcgt agcccttttc ttccaactca agcgccagat tggcatctcc ggtacgaacg
     8581 atttgaccat ccatcatcac atgaaccgcc tgaggtttta tataatccag cagccgctga
     8641 taatgggtga taatcaagac acccatatgg gtctgatcga ccaagatatt aattccttcg
     8701 gagacgattc gtaatgcatc aatatccaaa cccgaatcga tttcatccaa aatggcgatt
     8761 tccggttcaa gaatagccat ctgaagaact tcggtccgtt tcttttcccc acccgaaaat
     8821 ccgtcgttca aatatcgacc ggcaaaatcc ggatccattt tcagcatctt catcttttcc
     8881 tttagaagtt ttcgaaattc gggaatcgga atcccgtgat catccggatt cttgctttta
     8941 cggtgaatat ttaatgcggt ccgcagaaaa tttgccacgg tcaccccggg aatcgctacc
     9001 ggatattgga gtgccaaaaa caatccggcg tgtgagcgct tgtcgggggg ccaatcattg
     9061 attaattgat cgttcaagag aatttcacct tcgtcgggtc gatatgcagg atgtcccata
     9121 atcgtatagg agagggtaga ttttccggtt ccgtttggtc ccataatcgc gtgaatctcc
     9181 cctttgggaa cctgaatgga caggtgattg ataatctttt tatcatgaac ggtgacgctt
     9241 aagtctttaa cttctaagcc tatcattaaa ttctcctcaa atgcaccgat attggaatcg
     9301 gttgagtcat tttttcatct tttattcaac ggcagcgggg aaagagttaa taaacccgca
     9361 ttataaaatc atcgatttta ataacggata aattataaca gattgacgtc tttgaagtga
     9421 aagcttcgcc tcaagcggtt gaatttctaa cggaattcta agaaaggggc aaaccgatac
     9481 ggctctcatt tcaaatttat actctttttg aatcattatt gtgaaatccg ttagaattaa
     9541 gccgtattta tcgttgaaat ttatcaaatt caaggaaaaa gattaaggag gatccgatga
     9601 gaatcatttt tctgagacat ggtgaagcga tcgaacgagg cagtgtggat gatgaacaac
     9661 ggttcctcac agagagaggt aaaagagatc tggaaagcaa tctaccggcg ctcgccgctt
     9721 atttacgcaa ccgaaataat gtggaaatct ggtcctctcc gaaaatacga gcgattgaaa
     9781 ccgctcagat cttaacatcc ggtatggaga ataaagatat ccggtttaaa gattttatcg
     9841 gggacggaga tacggaacaa ctgaaatccg aattggagaa aaccggagct gagacggttg
     9901 ttattgtggg gcatcaacct tatttgtccg aatgggtgga tgaactttgc gattctgaga
     9961 tcctttttca aaaaggagcg gcggcggaaa tagagtatta tccggacagt gaccaaaaag
    10021 gcaaagtact atggcatttt ccgttgaaag gtttgaagca gttggctcga atgatggggc
    10081 cggaagttga aggaaattct tttcgacagg atctgatgat aaaaatggag caaaacttca
    10141 atcagatcgc agaaaatagg aatcgttttc tatccgaacc ggatgatgtc gaatcggcac
    10201 atcaactgag agtatctatc cgtagttttc gttccttgat atctctcgga aaaaaatatt
    10261 taaaaccgga aaaatatcag gattatcaag atcaatttcg gcaggcagct gctgagataa
    10321 gcactctccg tgaaattgat gtgatcatca atacttggaa cgaacactta ctcgatcaga
    10381 aactcgatcc gtctcaatct gtgttgatcc aggagttgaa tcgaaaacga aatatagaaa
    10441 aagatcgggt tataaaaatt gtatccaaga ataaatatat cgatcgcctg acaaaaagtt
    10501 ctgacgatct tataaagacc attcgaaaaa gcagcgaaca ggatgtcccg actcaaaaag
    10561 tgattgatgc ccggcttgac aaatggtatc aatatatttt gaaacggatg attaattata
    10621 atgaattcaa cttttcatat attcatccga tccgtctgaa atgtaaaaaa tatcgttatg
    10681 tcagcgaaaa cttttatggc atactggatc cggaacatcg aaaacgtcat cgggatgtaa
    10741 agcaactcca gacattattg ggaaatacct gtgatgcgat ccgtaaccaa gaagcgattc
    10801 tgaaaatcat cggtttgaga tctccggcgt tggagcagga agtgatcgct tttatcaatt
    10861 atgaaaaaga ggttgaatcg aattacagga aattatttga cagtcgcgaa tggcttcaac
    10921 aaattagcga aaaggaaaat taaattaaat cccggtttta tacttttatg cacattgtcc
    10981 cttatgggta tttttgattt cgtgaaaagg gctgataaaa taggcaaaaa ggaaaaagtt
    11041 ctctgtatac agaaccggat aaggaaggta tgaacgaaat ttttgaaaaa ttttcgatga
    11101 aagatcaaac cgcggtgata acgggcggcg gaggattgct cggcaaagaa ttttgcaaga
    11161 cgcttgcgca agccggtgca accatagtcg cggcagattt aaatctgaat ttagcccggc
    11221 aaacagtcga gttgattcaa gaattcggag ggcatggaat tgcctgcgct gtggatgtta
    11281 ccgatcccga atcggtgaaa gcgatggtcg atttggctgt caacgagacc ggacggttgg
    11341 atgtgctggt ttgcagcgcg gctttggatc cgaaatttga cggaacgcaa gggaaacatt
    11401 cgaatacatt tgaagaatat cctttgaaac aatggcggga tgctttggat gtcaacctga
    11461 ccgggctttt tctttgcgct caagcggcgg taaaaccgat gatcgcccaa aatcacggag
    11521 tcatcatcaa tatttgttcg acttacggat tggtcggtcc tgaccagcgg atctatgaaa
    11581 ggccggacgg ggatccgtca tcgaggcaat ataaacccgt ttattattcg gttacgaaag
    11641 ccggagtgct cggattgacc aaatacttgg ccacttatta tgccggcacg ggaattcggg
    11701 caaactgcct gacaccgggc ggtgtctata acggacacga cgaatttttt gttaagcaat
    11761 attcggcccg aaccgttatg ggtcgaatgg caaatatcga cgaactcagc ggagctttgt
    11821 tatttttggc atccgatgct tcttcttata tgaccggatc gaatttggtg gtggatggcg
    11881 gatggacggc atggtagcta agcacgcaga agttttggcg atcattccgg ccaggggcgg
    11941 atcgaaagga attccgcata aaaacattaa aaaatttgcc ggatatcctt tgattgcctg
    12001 gtctatcgca gccgcaaaac aatccgcatc ggttacccgg acaatcgtct ctacagacga
    12061 tgaggaaatc gcacgggttg cgcgcgaatg gggagcagaa acgccttttt tacggccggc
    12121 ggagtttgca atggatcaat cattggattt gccggttttt cggcatgcgc tggaatggtt
    12181 aaaagaaaat gaggggtatg aacccgatat cgttgttcag ctgagaccta cttcgccggt
    12241 tcggccggtg aatttagtgg atgatgccgt ccggctgctc acggatcatc cggaggccga
    12301 ttcagttcgc ggcgtagtgc cttcctctga gaaccctttc aaaatgtggc tgatcgattc
    12361 cgaaaccgga acaatgagcg gattgatcaa attagatggc attccggaac cctataatgc
    12421 cccgaggcag gtattacccg caacatattg gcaaaccgga catatcgatg cgatacgtcc
    12481 ccggcggact tttatggcgg gcgacagtat gagcggaaaa gtgattttgc ctcttttatt
    12541 ggatcccgct tttaaagtgg atatcgatac tttggcggac tgggaccgtt atgaacaact
    12601 ggtctattac ggcggtttgg atatggttta tcccgaacac aagagaagat cactgccgca
    12661 aaaaacgtgt ttattggtca tggacttcga cggtgttctg accaatgatg aggtcatcgt
    12721 gaaccaggat ggacttgaaa gtgtgatctg taatcgcggg gacgggatcg gtttttggct
    12781 gttgcgggaa gccggaataa ccgcgattgt gatttcaagc gagaccaatc cggtggtgcg
    12841 gaaacgctgc gaaaaattgg gaatacaatc ttatcagggg acgttagata aagcgactgt
    12901 gttgaagaaa tatttggagg agaataaaat tccggcggaa aatactgttt atatcgggaa
    12961 tgatatcaac gatttacaat gttttccgtt ggtaggttgt gcggctgctc ccgcagatgc
    13021 tcatgatctt gtaaaatcaa aagcagatat aattctcaaa cgaaacggcg gacaaggtgt
    13081 cgttcgtgag ttatgcgaat tgattgtccg gcgaaataag gaaagttatg ggaaagacag
    13141 ttaaattagg aaatcggtta gtcggcgaag gccaaccggt ctatgtcatt gcggaaatcg
    13201 gaatcaatca taacggagat attggcattg cgaaacgcat gattcgctcg gcttatgcgg
    13261 ccggagtgga tgcagtcaaa tttcagaagc ggactcccgc tgtttgcgtt cccctttcgg
    13321 aacaaagcaa aatgcgtgag actccttggg gttacatgtc ctatcttgag tatcgggaac
    13381 atgtcgaatt ttgggaagac gaatatcatg aaattgatga actgtgccat gagttgggaa
    13441 tcgattggtt cgcttcggta tgggatgtgg aatcaattga ttttttgatg aaatttgatc
    13501 ccattgccta caaagtccct tccgccagtc tgaccgatca tgaattattg gataaactgt
    13561 tgtcaactcg aaagccgatt attctgtcga ccggtatgtc tacgatggat caaatccgac
    13621 aatcggtgag tctgttcggg aatttggata atctgattat tactcatgcg accagtgctt
    13681 atccctgcaa accggaagaa cttaacctgc ggatgatccc aaaattaatc gaagaatttg
    13741 actgcccgat cggatattcg gggcacgagg tggggttgat tccttccgtt atttcggtgg
    13801 cattgggagc ttgtctggta gaacgccatt ttactttgga tcgggcaatg tggggaacgg
    13861 atcaagccgc ttccgtagaa cctcagggaa tggaaaagct ggtgaagtat attcgggtta
    13921 cggaagcttc tttgggcgac ggaatcaaga aagtgtatga tgacgaattg cccagcctga
    13981 aaaaattgcg ccgagtccaa taagttgtct tccatggcag tcgattcaag taagcgatct
    14041 gttttcatgc gaattcgcga tcgtcggcgc attcacgata cggatggcgc aaattctcgt
    14101 aaatttcatg aaattaaccg ttattcttca caaagagtcc ggaaggatga acaaccggtt
    14161 cttttcttca atgcttcgac gagattgacg cgtaccagtt taaatgccgc tttcgcctgg
    14221 ttggctgcga atagtttgca atctacgggt gtgccggtcg tatttatgac ctgtatgcgc
    14281 ggactgaatc cgtgtctgtt ggcaaccgat cgtgaaaatc cgaaaaaaga acttccctgt
    14341 caaagttgta ttcgtctgag taaaagcatg tataacgggt tacaaaccgt accgttagaa
    14401 tttcacgagg atccgatcct ctttaatgaa gtcgaagggt taacctatca cgagttggaa
    14461 tcctatgtcc atgaaggggt ccctctgggt gagctgacat tgccgtctct acgttggatt
    14521 atgagacgcc atcacttgga agatgttccc aatgccttgc cactccaacg actttatata
    14581 cgatcggcat ggagtactta tctcctggcg aaagaatgga tatcgaacaa ccatccgcgg
    14641 gcgattgtgg tttttaatgg gttacagtat ccggaagcaa ccgtgaaatg gatcggaaaa
    14701 caaaacggaa ttccgacatt cactcatgaa atcggattaa tgccggaaac agcgtttttt
    14761 acggaaggcg aagctacggc ttgtccgata gacttgccgg ccgatctgga aatgacaccg
    14821 gaaagaaata aaaaattgga tgattatttg gaaagtcgga tgaagggttc ttttcaaacg
    14881 gcaggcgttc gcttttggcc ggagatggag ggattgacac ccgaattttg gaaaatggtg
    14941 cggatgtttc ggcaagttgt accggtgttt accaacgtgg tctttgatac cagtcaaaaa
    15001 catgccaatg ttgttttcga taatatgttc gagtggctgg attcgatcaa gtcactgatc
    15061 gaaaagaatg cggacactct ttttgtaatt cgagcgcatc cggatgaact tcgtccggga
    15121 aaggaatcct tggaaacagt taccgattgg gttgtcggaa accacctgac ccatttgacg
    15181 aatgtcctct atatcgctcc ggatcaatat ttcagctcat atgatttgat tcggatcgcc
    15241 aaatttgtta tggtttacaa ttccaccatc ggtttggaag cggcggcaat gggggcggcg
    15301 gttttgagcg gcggaaaatc gcgatatacg caggtaccga ttacctattt tccgcaatcg
    15361 aaagaagctt tcattcaaaa agcgcaggat atgctggatg cggacgaatg ggttgttccg
    15421 gacgaatttc aacaaaacgc acgcaaattt atgtattatc aattctttca taccgcattg
    15481 gatttttccg agtttttatc tcccgatccc gtatggaacg gttacgtgaa attaaaagat
    15541 ttcaagccgg aatcgctgtt accggaaaat aatgagacga tgcgggttat tcaagatgga
    15601 attttgcgag gaaagccgtt cgtctaccct cattcgtttg aaaaagaccc gcagctcatc
    15661 aattcatccg ttcagatgaa gaacgataaa taaaggagaa aatttatgaa aattgttgct
    15721 tttgttccaa tgcgacataa ttcggtcagg gtcgaaggaa agaattacag aatgttagcc
    15781 gggaagccgc tttatcacta tattatggaa gcgctgctgg cggttcctga aatcaatgaa
    15841 gtggtggtcg acaccgacag tccggttgtc atcgaagggc ttgccaagga tttcccgaca
    15901 gtcaaatcat tgctgcgtcc cgaagcgtta cgagcggatg atgtcccgat gaacgaaatt
    15961 ttaatctatg atacaagcca agttcaagcg gattattatc ttcagactca cagcacaaat
    16021 ccgcttttaa aatccgaaac aatttcaaaa ggaattcgat cctttttgga aaaaacttat
    16081 ctatatgatt ccttattttc cgtcacgcgc gttcaaaaaa gattttggga tcagttagcg
    16141 aggccgatta atcataatgc atcgatctta ctgcgcactc aagacttacc gccgatctat
    16201 gaagagaatt cctgtatgta tttgtttact cgtgaaatat tgatgcgcaa acgtcaccgg
    16261 atcggtgatc gccccatgat gtttgaaatt ccggccatcg aagcgcagga tattgatgat
    16321 gaaacggagt ttaatatcac ggaaatgttg atgaagctgc gacaacaagg aaagttgtaa
    16381 gtttttttcg ggatataatg acttttactg aaaaacaaaa atgatgttgt gacggttctg
    16441 ccgtatgcat tttccacaga cgttatcgtt ctgtggcaga aaatttcttc aggagaataa
    16501 aaaataatga aacatacaat cattctatcc gcaccttata tgatgtcatc agtggaaaga
    16561 ttccgaaaag taattgaagg atattatggg atcaatatga tcgtcccgga agtaaaacaa
    16621 aaattaaccg aagatcaaat tatttcctac gccggtcaat ttgacggaac gatctgcggg
    16681 gatgatgttt acagcgcccg cgtgatcgaa gcttgtgttc ccaggttgaa agtgatttcc
    16741 aaatggggaa cgggcattga tgccatcgat aaagaagccg ctacccgatt gggagtcgtc
    16801 gtcggaaata cactcaatgc ttttacgatt cccgtttcgg agacagcgat cggatatatg
    16861 ctttcgtttt tccgtgcgat tccttttatg gatgactcga tgcgggaagg aatttggaat
    16921 aagccttcga caaccactct gtcagaaaat acaataggga ttgtcggggt cgggaatatc
    16981 ggacaagcgg tagtacgccg tttaagagca ttcggttgcc ggatcttggt aaatgatatc
    17041 gccgaaatta aaccggattt tttgatcgaa aatcgaatcg agtcggtttc attgccggaa
    17101 ttgttaagcc gttcggatat tgtcactatg catacggatc tgaatccgac cagctatcat
    17161 ctgatcaatc gtgaaacatt ggcgatgatg aaaccgacag cgattttgat caatacctca
    17221 cggggtccga tcgtggatga agccgcttta gccgaggcgc ttgaaaacgg gaaattgggc
    17281 ggagccgcat tggatgtttt tgaagtggaa cctttgcccg atgattctcc gttgcggaag
    17341 tttaagaatg tgttgttggc tccgcataac tcaaattaca gtccgatggc ttgtgaacgc
    17401 gttcattgga atacgattcg caactgtttg attcctttgg gttatgatgt ttccaaactg
    17461 gaagaactga aaaagacgat ctaagcaatt tcatttccga tatcggggaa aatgatttac
    17521 aaattttccc cgatttattg agtgagttat gtcgacatca aaaacgaata tgaaaaattc
    17581 tgttcccgaa aacatattac tgaaagggaa agagctttac gacgggataa agagactcgg
    17641 tgacattccg gaagcttatt tcgatccggt tcgtcgggat tccatggaac gtttgagtcg
    17701 attgaaagac agccgtaaag gggagcgttg ctttatcatg gggaacgggc ccagtttgaa
    17761 aaataccgat ctttccaaat tgaagaatga gtatactttt ggcttgaacc gtatttattt
    17821 ggcttttccg gaaatgggct ttgaaaccac ttattacctt tgtgtgaatg atctggttgt
    17881 cgaacaaaca tccgacgata ttcagaaatt aaagatgccg cgttttgtca cgactcgggc
    17941 gctgaaatgg ctgaagcctg aagaaaacct cttttttctt tattcgactt ataccggccc
    18001 gaccttcgcc accgatatcc gaaaaaggat gtgggaaggt gccacggtga cttatatggc
    18061 gcttcagacg gctttttatc tgggattccg gcaagtgatt ttgattggtg tcgatcacaa
    18121 tttccaaacc aagggaaagc ccaacgagac ggttgtttcc gaaggagatg atccgaatca
    18181 ctttaatccg ggttattttg ggaaaggctt ccgctggcaa ttaccggatt tggagacctc
    18241 cgaacgtgcc tatacaatgg cgcgtgaagc ctatcaaaag gcaggccggg aaatactgga
    18301 cgctaccatc ggcggaaaat tacaggtttt tccaaaagtc gcttatgact ccctttttta
    18361 aaaatcaatg agccatcctc atatttctgt tgtgacacca tcctacaacc aggcgccctt
    18421 ccttgagaaa accattcaat ctgttttgac gcaaaagaca cctgaaatcg aatattttgt
    18481 aatggatggg ggatcaacgg acggaagtat cgatattatt cgaaaatatg aatccgaaat
    18541 cgatcattgg gtatccgaaa aagatcgcgg acaggccgat ggaattaata agggcttttc
    18601 attggcgggt ggagaaatat ttggttggat aaattccgac gatttttatc tgaaaggtgc
    18661 ttttgaaaaa gtgctcgcct attttgatgc tcatccggag gtcgatttgg tatacggtga
    18721 tgtactttcc gtaaatcaag agggagaact gataaatgtg atgcgctttg cggattatac
    18781 attgcgggat ttaatggctt tccggatcat cagtcaaccg gcggttttct ttcgccgcaa
    18841 agcttgggac ggcgcaggcg gaatggattt acgctatcat tttcttttgg atcatcatct
    18901 ttggcttcga atcgcagcga gagggaaaat agcttatctg ccggaaccgt tggcggcggc
    18961 ccgtttttac ccggaagcaa aaaatcgcgc tcatgccgcg gatttcggag ccgaagctta
    19021 tcgaatcgcc gattggttgc tgacagatcc ggagatgaaa ccctattcga aaggatatcg
    19081 acgggttatt caaggcggag ccgattggct gaatgcgaat tatctgtcca acgggaatca
    19141 tccttggaaa tctctcaaga attatgcgaa agcatttacg aaataccctt cacgggtatt
    19201 tgaagatcgt cgtcgagtgg gggttacctt attgatggca atcagcccgg aaaaagcaac
    19261 cgatgtcttc cgccgggaag cacaaaagcg aatggatgct ttttccgctt atcgggatct
    19321 gttatgaatc ttcacacgat accttccgga aagaaattaa ccgggtgggc agagacggtg
    19381 attgttttga ttttaatggc ggcaggattt gctgtcaggt tatatgatct gaccgatccg
    19441 ccgttggatt tctattctcc gagacagttg cgctctgcca tcatcacccg ctctttttat
    19501 tacgaaagtc tgaacaacag tgatccgacc attgtcgaac aagccgataa actggccgat
    19561 ttggaacgct atgaaccgcc ggtgatggaa tggctggtat ccaaaatcga tttactgatc
    19621 gggaaagaag ttttttggac gggtcgcatt ttcagtgctt ttttctgggc gttaggcgga
    19681 ctgtttctgt atcggatctg ccgtcgtttt gcttcctttt cagccggaat attttcgtta
    19741 ttgatttatt attttgtccc gtttagtatt attgcttccc gatcttttca gcccgatcct
    19801 tggatgtgct tttgggtttt aggcaccgct tggatgttgt gcaactggtc tgagaaaggg
    19861 tcatggtctt cggccttttt ttccggattc atcgggggaa tggcgttgtt ggtaaagatt
    19921 tatgcgggct tttttgtcgc gtttatgtta ttggggatct tctgcgcaag tgttaaccgc
    19981 caaaatcgaa aacaaaggat atttcaaacc cttttgatcg gcgcagtcat gttgattccc
    20041 tcggcagttt actatttgat attcaatcct tcccgttcgg gagatttctt ttcattttgg
    20101 attgtttctc tttccggact gattcggacc tcaaattttt atgcggattg gattgcgatg
    20161 gtgaaagggc tgatcggatt ggtcctgccg ctctttgggt tgtggggagt ccttttcatc
    20221 cgaaatcgtc aggtaaaagg gttaatgatc ggttggtggt tgggatacat tttttacgga
    20281 ttatttgtcc cttatcagat cacgactcat gaatactata gcctgatttt gatcccattg
    20341 acagcgattt ccgtatgtcc ggtttcggat acgatttttg aaaaaactcg gcaaagtgga
    20401 aagattgcct tcgtttgttc gctccttttg attttggcgg cagccggtta cggcgcctat
    20461 tcggcttgcg gatatttacg agggacgaat tatgttttgg aaccggcgag ttggagaaag
    20521 gtcggggaag cgatcccgca aaatgagccg tttatcgctc ttaccggaga ctatgggatg
    20581 cgactgattt attacggctg gcggcaaccg tcccttactt ggccggatgc aaaggatgat
    20641 acgctgtttc aattggctgg gacgcctcaa gcggattttg atacttactt tgattcccag
    20701 acgaaaggca tgaattattt tgtggtgaca gcgctgggag agctggataa tcagccggat
    20761 ctaaaaaaaa gattggaatc ctttcctgtc tatgccgccg ggaatggata tattatctat
    20821 gatttgagaa ataaaccttg agaaatattg tcgcacggaa aaaattatct tcaatccaaa
    20881 tcattgtctt tatcctgata tttttatgcg ggactttttt gcgattcgtc gattttgacg
    20941 atccgccgtt ggattttcat ccggctcgcc aattacattc cgcattaatc gctcgcagct
    21001 attttctgaa agataacggg aatttacccg ggaaaagtgc cgattatcaa catgaggcgg
    21061 aaatgcgcgg ttcacaggaa cccagaatcg aaccgccgat tatggagaga ctcgctgcgt
    21121 gggggtataa aattgtcgga gatgccgacc tgcgggtacc gcgagcgatg tcaattgttt
    21181 tttggttaat cggcgggatc ggattatttc gattatgccg tcgatttctc ggaatcaccg
    21241 gcagtctggt cggtttcggt ttcttccttt tattccccta tacgattttt gcgagcagat
    21301 catttcagcc ggatcctttg atggtcgcac tgatgatatg gtcactgaat gcgttggtcg
    21361 aatgggattc tcatccggcg attgggccgg cgatcctgac cggggtcttg acggggaccg
    21421 cgattttggt caagcaggtg tgcgttttcc cattggcgtc agcctttgcg ttttacattt
    21481 tttcgtcttc ggatcggaaa caaaaaataa agagagttga tttttggata atcgtggctc
    21541 tttccgtcgc accggtattg atttataact actggggtta ctttgtcaaa ggttttttgg
    21601 tacaacaata tcaaggtcgt ttttctttga gcgattggtt ttctccggcg ttttatgtcc
    21661 gctggattcg gaagatggac gaggtgtttg gaatcgttgt cgtaacagcc gggttagtgg
    21721 gctgtcttgc ctgtgaaaat cgaagagcac gtttattgtg gatcggtttt tttgccggat
    21781 atattcttta cggtttggcg cttcctcacc atatcggaac ccacgattat tatcaattgc
    21841 ctttattccc gctgatttct ttcggaatcg gccgatcggc cgaatcaatc tttgaggttg
    21901 tcgggaggtt aaaagaaggc cggacgcttg cttatgtcgg aattactgtg tgcttgattc
    21961 tgttgggagc ttggtggagt gcggacagtg tcatcagctt gaaccgaagg gattatcgag
    22021 cttggccgga acgatggcaa tctctcgcgg ctgaattaga tccctacccg ggtcaaatca
    22081 acacgatcgg aattatggat gattatgggg cggcaatgat ctattggggc cttcggacac
    22141 cggtgatttg ggaacaaaat atcgaaaagc tttctgaaga ggaagccgac accatcattc
    22201 gccaggcgat gataaatcgg gaatacttaa ttgtgacgga ccttgacagc ttttatgcgc
    22261 agccgcggct tcagaattgg ttggttcgca atacgaagct ctatgcggaa gaaccggact
    22321 atctgattta tgatctgaga gggttaaatg agtgagcagg cggtttttcc gttcgtcagt
    22381 attgtcacac cttcgtttaa tcaggcggaa tttctcgaac gtaccattca atccgttctg
    22441 agtcaggatt atccgaacct tgagtacatc attatcgacg gaggatcgac cgacggaagt
    22501 gtcgagatta ttcggaaata tgagaaccgt ttggcttatt gggtttccga gaaggataaa
    22561 ggtcagacga atgccattaa taaaggcttt aatcgggcaa aaggagagat ttttgcctgg
    22621 ctgaattcgg acgatatcta tgagccggga gccattcaag ccgctgtgga agctctcatg
    22681 tcggatcctt ccttggggtt tgtatatggc gattgtaact ttatcgacag tcatgaccgt
    22741 attatcggga aattcgatgc gcgacaaacg gatctaaaaa aattacagcg cggatatgtt
    22801 catattcctc aacaagcttt tttctttcga ggggagctct ggaaaaaagt aggtccgctg
    22861 gatgaaagca tctattttgc catggattat gatttgtggt taaggttggc tcgaatcgca
    22921 ccttttcgct atataccggg aaaggtctgg gctgattttc gattgcatga agatgccaag
    22981 acgattaccg cggatgcgtc ttgttggccg gaaatgattc agattcgtcg tcgtgacggc
    23041 ggatcgaaat tttctgttct tttcctgaaa tatcaactgc gaaaattaat ggcgcctctg
    23101 attaattacc ggcgcagaaa aatgtttcga taaattttcc gatcaacacc tgattttaaa
    23161 taatttgtta ataccaaaaa tattgacaaa agtggggcgg gtgatatata ataatagtcc
    23221 gccttagaaa aagggttacg gtggtcttga aaaaaggaaa aaagatcgta atgacttttc
    23281 aaaaaagagc cttgacgaga tcaccgaaac acatataata agacgttgcg cctgggaaac
    23341 agaggggcgc gaagaacctt g
//
LOCUS       DAOSMM010000032        22919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2269_length_22919_cov_13.925953, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000032 DAOSMM010000000
VERSION     DAOSMM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 22919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22919
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2269_length_22919_cov_13.925953"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PK898_06090"
     CDS             <1..377
                     /locus_tag="PK898_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOU19640.1"
                     /translation="SVGKALRGKTELDSQGVADLFQAFFDGVQNRGKAQVGDKTFLDG
                     LYGAVESLKADAAAGVSMTEAAKRAAEAAHRGFLGTKGMIAQFGRAAGRGEQSRDLLD
                     PGAAVADLLMKGYAEFMSGKAE"
     gene            380..1405
                     /locus_tag="PK898_06095"
     CDS             380..1405
                     /locus_tag="PK898_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU19641.1"
                     /translation="MVTIKEIAAIAGVSPATVSLVLNNKEGVSEETRKHIRKVAEENN
                     YSLPQTRKKPLRGSIQFVKYSAHGMVVEENQGFIASIIDQIEAECRRCSYDLIMTGCN
                     RETAAETFRMVAAKPSDGIILLGSEMSDEQIRMMYQIQAPMIVIDNPMSYEHVDSVVM
                     ANEYIAYDAVNYLYQLGHRNIGYFHSSVDVANFGKRHVGYQTALEKLNLKSALIVSLT
                     PTLQGAYADMKMFLQQEHSQTAIKSISAVFADNDTVAIGAMKALQEFGYRIPDDLSII
                     GVDDIPFSAISTPSLTTMRISRSCIGKMAVDMLLKRIAHPDNPNVHIQISGQLVERHS
                     TRTYQAD"
     gene            1637..2101
                     /locus_tag="PK898_06100"
     CDS             1637..2101
                     /locus_tag="PK898_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOU19642.1"
                     /translation="MIIDHISHIEKYRSFGPGFESGIRFLLQHQNDPAEPVARYKLDE
                     NVDVMVNQYETKYPEESNFEAHNKYIDVQFMLKGDELVGWAPREKLSEISAAPEKDYY
                     ELFGESDYFPLSEGYFMIFFPEDAHQPGLVKDHPEQVTKIVLKVRVEGNLPL"
     gene            2252..2464
                     /locus_tag="PK898_06105"
     CDS             2252..2464
                     /locus_tag="PK898_06105"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOU19643.1"
                     /translation="MTDSVYKIIELVGTSTESWEKAAKNAIETAEKTLEDLRVAEVEK
                     MDIQLYEGSFVYRTKLKISFKYHSKE"
     gene            2621..3988
                     /locus_tag="PK898_06110"
     CDS             2621..3988
                     /locus_tag="PK898_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOU19644.1"
                     /translation="MCDFYLSSNLHGSVQTDQSKILFSPENNEENEIINIYPDVCFQT
                     FEGFGGAITDSSGYVFSLMNSIQKEQLLHQYFSAGEMNYQFVRIPIDSCDFSVDMYEA
                     MSDPDDTELKSFSFARTEKYILPMLKAAQQTAPQPLKLMLTPWTPPAFMKTNHDRCHG
                     GCLKPEYRSLWAKYICRYITEFRMRGWQVNRISIQNEPHAVQKWESCLFSAEEEKTFL
                     RDFLYPELLKQNLADIEIYIWDHNKERAYERAKAVIDSRTDEMISGIALHWYSGDHFE
                     SLDLLQKDFPDKKLIISESCLEYYKFGANLSDHSTQNLAHDLAGNMNHGLNAFYDWNL
                     LLDQHGGPNHAGNYCDAPMLYDTSSKQMNKRPSMVYYWHFSHFIHPGAVRIAFSRFTD
                     DIDVTAWKNPDKSIAIVLLNRNGVSTACNIRIHEKITKLTLPPYAIGTLVLRSGGSDC
                     SPELL"
     gene            4117..5088
                     /locus_tag="PK898_06115"
     CDS             4117..5088
                     /locus_tag="PK898_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU19645.1"
                     /translation="MSTLKDVAVLANVSPITVSRVINTPDLVKPGTRLKVQKAMDQLQ
                     YSPNIPAKNLVTKRSGIINVYIPENMDLSNPFSMHFIAGVSQGLGENMYSFLLLRDRK
                     IETACDGYIVTGLEHREIYEFDDFARKRNRPIVLFGHTDIEAIDCIDIDNVAGAFMAT
                     EYLIRNGHRKIAMINVNENRDYVTDRLNGYRKALDAYDIPYRKKRVIYAENNIHGGIK
                     AVEQLLESDRPTAIFCATDTMAIGVSRALSDAGLKIPEDISLIGYDGLGHQLLITPHL
                     TTIRQPVIQAGRLMAETLLQRLQGNSADRIVRMICPELLEGDSVAAI"
     gene            5136..6392
                     /locus_tag="PK898_06120"
     CDS             5136..6392
                     /locus_tag="PK898_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU19646.1"
                     /translation="MKKVLLFVLMVFCFVIPLTVYAEEVNTDLSGTLEVWSSGEELGR
                     FVEAFNKQYPNIKVNITVVPNADFVAKITPTLASGQGAPDIFTGESDYVKYLVNIGYW
                     DDLRADPYNCEKYTDDVWDYVKSVGTDSTGAIRALSWQAAPGSIIYRRDMAKDVLGTD
                     DPAELAKLLNSNEAMLDVAAKFKEKGIKMFASWKDIMNMQFSNRAQPWVVDGKLVIDD
                     SMLSFMDMAKTIVENGYDLNVDPWAPEWTAAVESDDTFCYVLPTWGYQFVVKPAAKKT
                     LGKWGLTEGPVPYVKGGTWLGIYKGSQKKDLAWAFLKFVTLDSEAQQNYAKQYGEYVS
                     LKSADMALAEGPGEEVLAGQNLFAFYNEEMKKIPNDLMTPYDGQINNAFLSNTQAYAT
                     GALDKDAAIKQFKEDVATAYPDLIVE"
     gene            6459..7361
                     /locus_tag="PK898_06125"
     CDS             6459..7361
                     /locus_tag="PK898_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU19647.1"
                     /translation="MIKAKKQRNKGYYGYLFIMPFFIIFAVFELYPIAYTFYLSFHKW
                     DGLMPSTYTGLNNFNRLIHDKIFFLTIKNTFRIWICNFIPQMLVAMILSALFTFNKIR
                     GMSFFRAVYYLPNLITAASVGLLFNLLFNGDKSVINHILIALGVKGAPFNFFQNKAFV
                     SGLVSYIQWWMWFGYTTIIVMAGITTIDSSVYDAALVDGATKFQTYRYITLPLIRKTI
                     IYLTITSIIGGMQLFDVPATMTNGSGDPMKSVLTTSMYIYNQGFKSHNYGYASMVSVG
                     LFLIIAVLSIFALQTMQKKESEND"
     gene            7354..8184
                     /locus_tag="PK898_06130"
     CDS             7354..8184
                     /locus_tag="PK898_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU19648.1"
                     /translation="MTDKFKSYFSKISLYLFLVILLLLSLIPFYLMIINGTHKSFDIV
                     TKLNLFPGHNLSENYETLQSHVNIWQGFLNSLCIAVPFTILSGYFGALTAYGFAKYHF
                     PGEKILFSIVLASMMLPPQLSIIGFYQLNLKLKLLNSYFPFILPGIANATTVFFMRGM
                     IEQGIPDSIMEAARLEGCSEFKIFNRIVLPCVMPGVATMCIFNFVSCWNNYLGPLIIM
                     TESKKYTLPVMIAMIKGLYLSNYGAMYLAIAISIVPIIIVYAFCSKFIINGLTAGSEK
                     "
     gene            8194..9540
                     /locus_tag="PK898_06135"
     CDS             8194..9540
                     /locus_tag="PK898_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19649.1"
                     /translation="MTQIKWFFSNNAVQWQQKTPTCFTGEWNLSTDGREYQKMFGFGG
                     CFNEIGFQTLKQLSDTEQKDVMKDLFYPSENGCNFSLCRLPIGANDYSFDWYSLDETP
                     GDYELADFSIERDKKALIPYIRAALAYLPDLNLFASPWSPPTWMKHPPVYNWGKLIFT
                     KEIQTAYAFYFVKFVEEYQKMGIKIDQIHVQNEPVANQKFPSCMWTGTELRDFIRDYL
                     GPAFRKAGLSTEIWLGTINAPGCDYRKMIFDKWADEDYDFFANTVLRDDSARQYISGV
                     SYQWGGKIAIQRTFESWWPRIRLMQSENECGFGDNTWKNALYNWTMMKHYIGNGAESY
                     IYWNMILPPDGVSTWGDPQNALITVNPTGPKIIKNPDYYLMKHFSSFIKRGAIRLGIQ
                     GDMAADSLIFHNPDGSFVIEAMNPFETELILKLNLSGIEAGFELEPKSLNSIIIDG"
     gene            complement(9653..18571)
                     /locus_tag="PK898_06140"
     CDS             complement(9653..18571)
                     /locus_tag="PK898_06140"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOU19650.1"
                     /translation="MVEENQKKQERDQNDFSNLNPSELKSRQIQELVTSLTDVCQQVQ
                     FQKPTRKAQSALPGRDIPGKNLSQFEAWLDEAHHYFSESSQNKIPLTYASEWILDNYY
                     IIRQTLQQIKEDLPVSFYQQLPKITNGVLKDFPRIYIIARSILSYQKLMIDPMELESI
                     LIQFQKVVPLSTGELWAFPIFLRYSLIEFLSIELVTIIHPNDQPNLPLVFSQNMETSC
                     QFSEENGAFTRTVNNDSVAKIIISLRTISEQDWNEFFESVSLLEELLRKDPSGIYPRM
                     DFKTRDHYRKEIEKLSRATGQDEIKIAETILNMATDINQGQSDSPLYFETLIDKNKDR
                     IDCSGKNPGLHIGEIIIGNLRPQLEQKIGYRPNFKTRLQRWIFKHADFVYLSAIFLLT
                     ILLLGFVSAITNLPQQFNAGSSLNPGTVAHYRPVQWLAIILYILVGIVPAMTVATGLI
                     NWLITLLVHPRTLPKMNFRNGIPYSFQTLVVIPSLITSRKDIESLIQQLELHYLRNPE
                     PGLFFALLTDFKDADQETMPEDEDLVKQTIEAIGALNRKYGPVSFSENSAENSEENDS
                     TSVSRFYFLHRKRLWNPSEGKWIGWERKRGKLHELNLLLRGKSGLSFTVFTDGFHDEN
                     PDKGILQRTRFVITLDTDTVLPRGAACRLAGTLAHPLNRPIFDEKTGQVISGYTILQP
                     RMEIHPRSANYSWFTRIYSGDTGLDLYTLAVSDAYQDLFGEGIYVGKGIYDVDGFTRS
                     VEGHIPENSVLSHDLLEGLMGRAGLVTDITMIEDYPQNYILQALRQRRWIRGDWQLLP
                     WLIHPRRFGVTFSIIDRWKIFDNLRRSLFPPALSLFFILGVIFLPRFALLWSLTVLVS
                     LGIPLITSFINSIIQIVHGGTVRAAFRPLFWNFLRWLLAVAFLPYEAYYSTDAIFTTL
                     YRLFISKKDLLQWTTAAQTAHLFGLQRKNISWRKMTGVAGVSLLLTILLQFIWGFSGN
                     GLAPAIINASPVLLLWLLSPMIAWWINLPIIKKSAKLNPDQIKLLRLVSRRTWGFFER
                     FVGPEDHWLPPDHFQEKPIGTVAHRTSPTNIGLLLTSTLAAYDLGYLDQRTLAIRLAT
                     TLETLSQLERFRGHFLNWYDTITLQPLNPRYVSTVDSGNLAACLMITSQACKNKRNET
                     ILRWNLWEGYLDSLGNLADGLNAIRKKDFDRQIKEIDALIKGISNEILAARSHPENWY
                     PLFLKVKGFFWQDISNRLQQLITVGGSSFDLETLNKLQEFSRQIEQHNLAIERTITEL
                     LPWIPLFENPPRLFRQPQFSEAMNSLRSCLPYDLAFGQLHDQIERASLYIEDLKDCLS
                     EKSEASQAPEDSEESDRNIAWEWLNQLITLLDHAEVNANGMIAQYENIMAKSEQLMNE
                     MDFGFLYNKQRHVFHIGLNLVTGQLDQNFYDLLASEARIASIIAIAKGDVPQSHWMQL
                     GRPITRIKNLYVLLSWSGTMFEYLMPALFFHSYPATLLAESSEGAVLRQIEYGKEKGV
                     PWGISESGFYRFDASQNYQYRAFGVPGLGFKRGLADDLVIAPYASLMAVSYEPEAVVQ
                     NLVRLLEYKMFGLFGLYEAIDFTPDRLLKDERSALVYEYMAHHQGMIMMAMANFFAND
                     IMVQRLHRDSRIQSVELLLQEQIPYFVPIQNPDAENVEGLQRMVAISEEIAPWRVPLL
                     STIPQLNLLSNGSYHLLISNMGGGYSSMNAVDLTRWRADQVTDSWGTWIYIQEMDSNS
                     GQPGRFWSATYQPVPGDPTNLQVTYYAHMAVFRRSENGFISTMDVTVAPDDPVEIRRI
                     HLMNTQDDIHTLRLTSYGEVILSSQLTDVRHPAFNKMFIESEFIPELNLQIFTRRLRS
                     DQEKSIFLGHMVLTKGIQKTVSHEADRYHFIGRGGSTRNPVALSSEQYLSGTTGATLD
                     PIFSLGQEVELLPHGSTDLAFLTFTAESREELLILADRYRNWAAIETAFSQANIAAKA
                     WLGKLKIGSQSLETGLHLLSALNYPNKIFRAPSEVIAANCLGQPALWRFGISGDYPIL
                     LIEVDDPQQIDLVQEALKIYRFLKTHLFDLDLVILNLQPTDYGAELNGVIQRTISKTN
                     SEDWLNQRAGIFVLHGDQVTQEELTFIRTAASAIFYGDKGSIREQMPEYVIPEHHLPD
                     LIPTRIKEKPELACQIPYTYPLKEIGDLQRYNGFGGFADGGKEYVIDWNDLQRNAAEF
                     SQKKFTPAPWVNVIGYPNFGFMVSETGSQCTWAFNSGENRLTPWSNDPVIDPSGEILY
                     LRDEETGEIWTPTPLPAGSDQPYRVTHGAGYTVFEHNSHGLNQVLTLFASPEDPVKII
                     DLKIQNTLDHPRRITATQYVEWVLGTTHEANMAYIIPEYDSEHECLMALNPHNPEFGS
                     HVAFLIASKNLHGLTADRSEFLGRAGSFANPAALRRLGLETHITPGDDPCAALQVHLD
                     LLPGAVEEIYFVVGEGNNKEHCIELAQKYHNPNNVNAAFEHTHLFWDNLLHSIRVKTP
                     QPATDIILNHWMLYQALSGRIWGRTGFYQSSGAFGFRDQLQDVLAVLPIDPTITRNQI
                     LISASRQFEEGDVLHWWHPPFNRGVRTRFSDDLLWLPYVTAIYIESTGDNSLLEEKVP
                     FLSAPPLAEGELERYADYRSTEQSYSIMDHCLRAIEKGMTAGSHGIPLIGTGDWNDGM
                     NRVGEKGQGESVWLAWFLCDVLQRFAEICDKQGYPDRGQKYRLKAIEYAAAVEKFAWD
                     GAWYRRAYTDEGTPIGSSLNQECQIDSIAQSWAILSGAGNDERSRRAMMSVMENLVRF
                     DDRLILLFTPPFDKSSVDPGYIKGYPPGTRENGGQYTHAATWTAWAFAALGDGKQAGH
                     LFELLNPIFHSDNEEKATVYRVEPYVICADIYSQPPFTRRGGWTWYTGSAAWMFRLGI
                     EAILGFHKIGNSLQIDPVIPPEWDEFEIQYRFGKSTYFILVRNPDHVSRHVRTVQSDG
                     KQIDPMSIPLVDDGLIHRIEVTMGEIKQ"
     gene            complement(18571..19659)
                     /locus_tag="PK898_06145"
     CDS             complement(18571..19659)
                     /locus_tag="PK898_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU19651.1"
                     /translation="MFKRILVPLDGSALAEQAIYHAEQFARIFGSKIILLQVLDPASF
                     RENPISVDPLNWQIRKKKAEIYLEEVTKRMNADLLEDPMPAVDFENPETKSPKSRVEY
                     AISEGKTAESIVNFAHQENIDLLIISTHGAGGLSRWNISSITQKVIDMIYLPILLIRS
                     YYQPKLVVNRVHYRRILLPVDSSRRAECALSVGLALARGEIPEGFASELNSRKPIQTA
                     KTIPSLPTQLVLIAVIKPPEIPIPEPYPPEIEQLTKKLMTVSRRVVSEYLEETKARLQ
                     VDCETYVLESSSVSSAIQNFANQEENIDLVVLCAHGYTGQIEWPYGSIARNYIEYGTK
                     PVLIIQDVPRSEFQPTAAEIAFKKVGGR"
     gene            complement(19876..19949)
                     /locus_tag="PK898_06150"
     tRNA            complement(19876..19949)
                     /locus_tag="PK898_06150"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19913..19915),aa:Lys,seq:ctt)
     gene            complement(20006..21997)
                     /locus_tag="PK898_06155"
     CDS             complement(20006..21997)
                     /locus_tag="PK898_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HOU19652.1"
                     /translation="MEKYTEEPTLDLKYLDLLAEKYPNIQEASTEIINYQALLQLPKG
                     TEHFMSDLHGEYEAFIHIMNNASGEIRKKVDSLYGKMLTSAERAQLSTLIYYPEEKLK
                     EIKNNQEININEWYMITIHQLIEIAHLVSIKYSRNKVRKSLPPEYAFIINELINAENT
                     SREEYYESIISTIIEIGKAEDFIVAFSTVIKRLIVDQLHIVGDIFDRGPRPDIIIDSL
                     RAHHSVDIQWGNHDVLWMGAASGSAACIATVLSNSIKYGNLDTLETAYGINLRPLALF
                     AQQQYKGSKRYELKSNDEIGNEDLRLLSKMYEAISVILFKLEGQIILRHPEYNMDDCL
                     LLNHINRKKGTIWIAGKTYPIKINDLFPMDEENPYQLTSDEQSVLDQLFTTFKNSYRL
                     QQHIRFLYSAGSMYLQCNNNLLFHGCIPMNPDGTFMNFCVNDVNYHGKSYFDYADKMA
                     RQAYYAPEGSKEKKDGRDFLWFLWRGRHSPLFGRDRITTFERLYVDDPEAWIEEDNAY
                     YKLIEDPVICRKIFEEFGLDYETSHIINGHVPVNAIMGESPIKANGRIIVIDGGFCKA
                     YQKKTGIAGYTLIYDSYGMRISSHEPFLGIKNAIENNSDIFSQEQVFETSDNRLTVAD
                     TDGGKDLIHKIEDLKNLMSAYQQGLIKERRGIPADNTTS"
     gene            complement(22296..>22919)
                     /locus_tag="PK898_06160"
     CDS             complement(22296..>22919)
                     /locus_tag="PK898_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU19653.1"
                     /translation="SITANLEFCSTKRVVTVYTAKGINGCFFDHGQMIEGSNSIIGEF
                     GHMILKPDYPVSCICGKHGCFESIVKPESIRSRLKQGEDLDSFLSFVQKPLKEIMFDD
                     VVKGFEKGFACCQSELKTLAACFGQAFLNISLVYDPDVFIIEGFFSGFNDAFETELYN
                     YLSDFSLLTPDRYFRIVSDPQPLPKLEVAGAITALKEYFFSDDLLYV"
BASE COUNT         6573 a         4712 c         5078 g         6556 t
ORIGIN      
        1 tgtcggtcgg aaaagcactc cgcggaaaaa cggaacttga cagccaggga gtggctgatt
       61 tgttccaagc tttttttgac ggtgttcaaa atcgcggaaa agctcaagtt ggtgataaaa
      121 catttttaga tggtctttac ggcgcggtcg aatccttaaa agcggatgcg gcagccggag
      181 tatcgatgac cgaagcggcg aaacgagctg cggaagctgc ccatcgggga tttctcggta
      241 cgaaaggcat gattgcacaa tttggcagag ccgccggcag aggggaacag tctcgggatc
      301 ttttagatcc gggggcggcc gtagctgatt tgctgatgaa gggttatgcg gaatttatga
      361 gcggaaaagc ggaataaaat tggtaacaat taaagaaatc gcagctatcg ccggtgtttc
      421 acccgctacc gtatccttgg ttttgaacaa caaggaaggt gtgagcgaag aaacccgtaa
      481 acatattcgg aaagtagcgg aagagaataa ttattctctt ccgcaaaccc ggaaaaagcc
      541 gttacggggg agtattcaat ttgtaaaata ctccgcacac ggaatggtgg ttgaggagaa
      601 tcaaggtttt atcgcttcca tcatcgatca aatagaggcg gaatgccgtc gttgttctta
      661 tgatttgatt atgaccggat gcaaccggga aacagcggcg gagacatttc ggatggtcgc
      721 ggcaaagccc tctgacggca ttatattgct gggttcggag atgagcgatg agcaaattcg
      781 gatgatgtat caaattcaag ctccgatgat cgtcatcgat aaccccatga gttatgaaca
      841 tgtggacagc gtagtgatgg cgaatgagta tatcgcttat gatgcggtga attatcttta
      901 tcaactcgga cacagaaata tcggatattt tcacagcagc gtcgatgtgg cgaatttcgg
      961 aaaacgtcat gtcggatacc aaaccgcatt ggaaaaattg aatctaaaat ctgctcttat
     1021 cgtttctctg acgccgactc tgcaaggcgc ttatgcggat atgaaaatgt ttttgcagca
     1081 agaacattcg caaactgcca taaaatcgat ttcggctgtt tttgcggata atgatacggt
     1141 tgcgatcggc gcaatgaaag cattacagga gttcggctat cggatcccgg acgatttgtc
     1201 gataatcggg gtggatgata ttccattcag tgcgatcagc acgccatcgt tgacaacaat
     1261 gcgtatttca cgatcctgca tcgggaaaat ggccgtggat atgttattga aacgcattgc
     1321 tcatccggat aatccgaacg ttcatattca gatttccggg caattagtgg aacggcacag
     1381 tacaagaaca taccaagctg attgaaaatc gaaggacttt tgactcgaat cggtcctttt
     1441 tcatccggat cgtttgaaga tgaatatttc aataggtttg ttggatgaag aatttttatt
     1501 ttcaacagat ttattcccat tcttattttt tatacacaaa taaaaaaaat catggaaaga
     1561 attgtccgtg ggttataatt tttaaactct ctaaattgaa tgaggagagt ccattcacgg
     1621 aacgaaggag gcattcatga ttattgatca tattagccat atcgaaaaat accgatcttt
     1681 tggacccggg tttgaaagcg gtattcgttt cttattacag caccaaaatg atccggcaga
     1741 accggttgcc cgttataagt tggatgaaaa tgtcgatgtc atggtgaatc aatatgagac
     1801 gaaatatccg gaagaaagta attttgaagc ccataacaaa tatattgatg ttcagtttat
     1861 gctgaaagga gatgaattgg tcggctgggc acctcgggaa aaactctcgg aaatcagtgc
     1921 ggcaccggaa aaagattatt atgaattatt tggcgaaagt gattatttcc cgttgagtga
     1981 gggatatttt atgatctttt tcccggaaga tgcacatcaa cccgggctgg tcaaggatca
     2041 tcccgaacaa gtgactaaga tcgttctaaa agtacgggtg gaagggaatt tgcctttata
     2101 aaaaacggca tttgagttgc ttattgtaat aaattctttt tcatgtatga taaagatatg
     2161 aaacgataat tttgttgaaa atcggagagg ctttcatatc aaacgggatc aattcctcaa
     2221 aaagagggaa aacgaaagga gtgtaactca aatgaccgac agtgtctata aaatcatcga
     2281 attagttgga accagcaccg aatcctggga aaaagcggcg aaaaatgcaa tcgaaacggc
     2341 tgaaaaaacc ttagaggatt tgcgggtggc tgaagtcgaa aaaatggata tacagctcta
     2401 tgaaggttct tttgtttatc gtacaaaact aaaaatctct ttcaaatatc acagcaaaga
     2461 ataatatttt tgtcgcaaaa aaatcgatca agaaacccca tcaaatggtg gggtttcttt
     2521 ctgctcggac ttgagtgaaa aactgtttcg gatatcaaat gatataatca gccagtcaat
     2581 tttgaatgca aaaataattc ggaggtgaaa atattttcat atgtgtgatt tttatttatc
     2641 aagcaacctt cacggcagtg ttcaaacaga tcaatctaaa atcctttttt ctcccgaaaa
     2701 taacgaggaa aatgagatta tcaatattta tcccgatgtt tgttttcaaa catttgaggg
     2761 ctttggcggt gcaataacag attcatccgg atatgtcttt tctttgatga attcgataca
     2821 gaaagaacag ctgttgcatc aatacttttc tgccggagaa atgaattatc aatttgttcg
     2881 gattcctatc gacagctgtg acttttcggt agacatgtat gaagcgatgt cggatcctga
     2941 tgatacggaa ctgaaatcat tttcattcgc cagaaccgaa aaatatatct tacccatgtt
     3001 aaaagcggcc caacaaaccg ccccccaacc cttgaagctg atgttgacac cttggacacc
     3061 gccggcattt atgaagacaa atcatgatcg ttgtcatgga ggctgtctga aaccggaata
     3121 tcgttctttg tgggctaaat atatttgtcg ctatataacc gaatttcgga tgcgaggttg
     3181 gcaagtaaac cggatttcga ttcagaatga accgcatgct gttcaaaaat gggaatcgtg
     3241 cttgttctcg gctgaagaag aaaaaacttt tctccgtgat tttctgtatc cggaacttct
     3301 caagcaaaat cttgcggata tcgaaatata tatttgggat cacaacaaag aacgggcgta
     3361 tgagcgggct aaagcggtaa ttgattctcg gacagatgag atgatcagcg gtattgccct
     3421 tcattggtat agcggtgatc attttgaatc attggattta ttgcaaaaag attttccaga
     3481 taagaaattg attatttcgg aaagttgtct cgaatattat aagtttgggg caaatctttc
     3541 tgatcattcc actcaaaatc ttgcccatga cctggccggt aatatgaacc atgggttaaa
     3601 tgctttctat gattggaatt tattgttaga tcaacatgga gggcctaatc atgcggggaa
     3661 ttattgtgat gctcccatgt tgtatgatac ctcatcaaag caaatgaaca aacgaccgtc
     3721 tatggtctat tattggcatt tttctcattt tattcatccc ggcgctgtcc gaatcgcatt
     3781 ttcccgattt acagatgata ttgatgttac tgcttggaaa aatcccgaca aatcgattgc
     3841 gattgtgtta ttaaatcgca atggtgtatc aactgcttgt aacattcgaa ttcacgagaa
     3901 aattacaaaa ctaacgcttc ctccctatgc aatcggcact ctggttttac ggagcggggg
     3961 atcggattgc agtcctgaat tattgtaagt attttcaggt attgcaaaac caagaattgt
     4021 gtgatataaa aatgatagcg ttatcattaa gcaagatcga acaaaatttt ttactgtaaa
     4081 aaatgatagc gctatcatta gcgaggatgg attaatatgt cgactttaaa agatgtcgca
     4141 gtgctggcca atgtttctcc gatcaccgta tcccgtgtta taaatacgcc ggatttggtg
     4201 aagccgggaa cccgcttgaa ggttcaaaag gcgatggatc aactgcaata ttcgccaaat
     4261 attcccgcaa agaatctcgt taccaaacga tccggaatta ttaatgtata tattccggaa
     4321 aacatggatt taagtaatcc gttctcgatg cactttattg ccggggtcag tcagggctta
     4381 ggggagaata tgtattcttt tcttttattg cgtgaccgaa agattgaaac cgcatgcgac
     4441 ggatatatcg tgacagggtt agagcatcga gaaatatatg aattcgacga ttttgcacgc
     4501 aaaagaaacc ggccgattgt tctttttggt cataccgaca tcgaagcaat agactgcatc
     4561 gatattgata atgtggcagg tgcttttatg gcaaccgaat atttaatacg gaatggtcat
     4621 cgtaaaattg ccatgataaa tgtcaacgag aatcgcgatt atgtgactga tcgtttaaac
     4681 ggctatcgta aggcgcttga tgcatatgat attccatatc gaaaaaaaag agtgatttat
     4741 gcggaaaata acatccatgg cggaattaaa gctgtggaac agttgttgga gtctgatcga
     4801 ccgacggcga ttttttgcgc caccgatacg atggcaatcg gcgtttctcg agcgttatct
     4861 gatgccggct tgaaaattcc ggaagatatt tcattaatcg gatacgatgg cctcggacat
     4921 cagttgttga tcactcccca tttaacaacg atccgccaac cggttattca ggcaggtcga
     4981 ttgatggctg agacattgct tcaaagattg cagggtaatt ctgccgatcg gattgtgagg
     5041 atgatttgtc ccgagttact tgagggagat tcggtagccg cgatatgaat cgaataccga
     5101 tccgaagcag gatagaaaca aaaaaaggag aaaatatgaa gaaggtttta ttgtttgtat
     5161 tgatggtttt ttgttttgtt atacctctta ctgtttatgc cgaagaggta aatacggatt
     5221 tatcggggac attggaagta tggtcctccg gtgaagagct tgggcgattc gtcgaagcat
     5281 ttaacaagca gtatccgaat attaaggtga atattacggt ggttccgaat gcggattttg
     5341 tcgctaaaat aacgccgact ttagccagcg gtcaaggtgc tcccgatatt tttaccggcg
     5401 aatcggatta tgttaaatac ctcgtcaata tcggttattg ggatgatctc cgagccgatc
     5461 cgtacaactg tgaaaaatac accgatgacg tatgggatta cgtgaaaagc gtcggcacag
     5521 attcgaccgg tgcaattcgg gcactcagct ggcaggcggc accgggttcg attatttacc
     5581 gaagggatat ggctaaggat gtcctcggta cggatgatcc cgccgagttg gcaaaattat
     5641 taaacagtaa tgaagcgatg ttggatgtag ccgctaagtt taaagaaaaa ggcatcaaga
     5701 tgtttgcgag ctggaaagat atcatgaata tgcaattttc caatcgcgct cagccttggg
     5761 tcgtcgacgg gaaattagtt attgatgatt ccatgctcag ctttatggat atggctaaaa
     5821 caatcgttga aaatggctat gatctcaacg tggatccctg ggcaccggaa tggaccgccg
     5881 ctgtggaaag cgatgatact ttctgttatg ttttgccgac atggggttat cagtttgttg
     5941 taaaacccgc agcaaagaaa acactcggga aatggggttt aactgaagga ccggttccgt
     6001 atgtaaaagg cggaacgtgg cttgggatct ataaaggcag tcaaaagaaa gatcttgcct
     6061 gggcattcct gaaatttgtg accttagaca gtgaagccca gcaaaattat gccaaacaat
     6121 acggagaata tgtttctctg aaatccgcag atatggcttt ggcggaagga cccggtgaag
     6181 aagtgttggc gggacagaat ctgtttgcat tttataatga agagatgaaa aagatcccca
     6241 atgatttgat gaccccttac gatggtcaga tcaacaatgc atttctgtct aatacacaag
     6301 cttatgcgac cggtgcgtta gataaagatg cagcgattaa acaattcaag gaagatgttg
     6361 ccacagcata tcccgacttg attgtcgagt aagatttaaa gatgagacag gccgccggca
     6421 tcaactgacg gcctgctttt ttagggatga ttcggaaaat gatcaaagca aagaagcagc
     6481 gaaacaaagg atattacggg tatcttttta ttatgccgtt tttcattatt tttgcggttt
     6541 ttgaattgta tcctattgca tatactttct acctttcatt tcataaatgg gacggcttaa
     6601 tgccatcaac ctacaccgga ttaaataact ttaatcgatt aattcacgat aaaatctttt
     6661 ttttgacgat caagaataca ttcagaattt ggatctgtaa ttttattccg cagatgcttg
     6721 ttgcgatgat tctttcggct ttatttacct tcaataaaat tcgcggaatg agttttttcc
     6781 gtgcggtata ttaccttcct aatttaatta cagcagcttc ggtaggatta ttattcaatc
     6841 ttcttttcaa cggagataag tcggttatca atcacatatt aatcgctttg ggtgtaaaag
     6901 gagccccttt taattttttc caaaataaag ctttcgtctc cggattggtc tcttatatcc
     6961 aatggtggat gtggttcgga tatacgacga tcatcgtgat ggccggaatt acaaccatcg
     7021 attcttcggt ctatgatgcg gctttagtcg acggagcaac gaaattccag acctatcgct
     7081 atataacgtt gccactcatc cggaaaacaa tcatttattt aacaattaca tctatcatcg
     7141 ggggaatgca gctttttgat gttccggcga ccatgacgaa cgggtccggc gatcctatga
     7201 aatcagtgtt aaccacatcg atgtatatat ataatcaagg attcaaaagt cataattacg
     7261 gttatgcatc gatggtcagt gtcggtttgt tcttaataat tgcggtcctg agtatttttg
     7321 cgctgcaaac gatgcaaaag aaggaaagcg aaaatgactg ataagtttaa aagttatttt
     7381 tcgaaaataa gcttatatct gtttctcgta attcttttgt tgctttctct cattccgttt
     7441 tatttgatga ttattaacgg aacacacaaa agctttgata tcgtaacaaa gctcaactta
     7501 tttccgggac acaatctctc tgaaaattat gaaacattac aatcgcatgt caatatttgg
     7561 caaggattct taaacagtct atgcattgct gttcccttca cgattttgtc aggatatttt
     7621 ggggctttaa ccgcttatgg gtttgctaaa tatcattttc ccggagaaaa aattctcttt
     7681 agcatcgtat tagcatccat gatgttacct cctcaactca gtattatcgg attttatcaa
     7741 ttgaatctga agttaaaact gcttaattca tattttccgt ttattctgcc gggaattgca
     7801 aacgcgacaa cagtcttttt tatgagagga atgattgagc aagggatacc tgattcgatt
     7861 atggaagcag cccggcttga aggttgttct gaatttaaga tatttaatcg gattgttttg
     7921 ccgtgtgtca tgccgggtgt ggcgaccatg tgtattttta attttgtttc ctgctggaat
     7981 aactacttgg gtccattaat catcatgaca gaaagcaaga aatatactct gccggttatg
     8041 atagccatga ttaaagggct atatctttca aattatggag caatgtatct cgcaattgcg
     8101 atttccattg ttccgatcat catcgtgtat gcattttgtt caaaatttat tattaatgga
     8161 ttaacagccg gttcggaaaa ataaagggaa gaaatgactc aaatcaaatg gttcttttca
     8221 aataatgccg ttcagtggca acaaaaaaca ccaacctgtt ttacagggga atggaatctg
     8281 tcgacagatg gtagggaata tcaaaaaatg ttcggttttg gaggatgttt caacgaaatc
     8341 ggttttcaaa cattgaaaca actttccgat accgaacaaa aagatgtcat gaaggattta
     8401 ttctatcctt ccgaaaacgg gtgtaatttt tctttatgcc gtttgccgat cggtgcaaat
     8461 gattattcgt ttgattggta tagtcttgat gaaacacccg gtgattatga attggcggat
     8521 ttttcgattg aacgcgataa gaaagcattg attccatata tccgagcggc gcttgcctat
     8581 ttgcccgatt tgaatctgtt tgcttctcct tggagtccgc cgacatggat gaaacatccc
     8641 cctgtatata attggggaaa attgattttc acaaaagaaa tacagactgc ctatgccttc
     8701 tattttgtaa aatttgttga agaatatcaa aaaatgggaa taaaaatcga tcagattcat
     8761 gtacaaaatg aacctgtggc aaaccaaaaa ttcccttctt gtatgtggac gggaacagaa
     8821 ctgcgtgatt tcattcgcga ttatcttgga cccgctttcc gaaaagcagg cttatcaacc
     8881 gaaatctggc tgggaacaat caatgctccc ggatgtgatt accggaaaat gatttttgat
     8941 aaatgggctg atgaagacta tgattttttt gcaaacaccg ttttacgtga cgattcagcc
     9001 aggcaatata tcagcggtgt cagttatcaa tggggcggga aaatcgcaat ccaaagaact
     9061 tttgaaagtt ggtggccgag aatccgattg atgcaatcgg aaaacgaatg cggtttcggt
     9121 gataatactt ggaaaaatgc tctctataac tggacgatga tgaagcatta tatcgggaac
     9181 ggggcggaaa gctatatcta ttggaatatg attttgcctc cggacggcgt gagcacttgg
     9241 ggagatcctc aaaacgcact cattaccgtc aatccgaccg ggccgaaaat aattaagaat
     9301 ccggattatt atctgatgaa acatttttca tcatttatta aaagaggagc gatccggttg
     9361 ggaattcaag gagatatggc agctgacagt ctgatttttc ataatccgga cggaagcttt
     9421 gtcatcgaag cgatgaatcc gtttgaaacc gaattaatct taaagctgaa tctttcggga
     9481 atcgaagccg gatttgagtt ggaaccgaaa tcattgaaca gtatcatcat tgatggttga
     9541 cactttgttt acggttatga atctccgaaa gtaatattac aaaatagatc cgattacaat
     9601 ataaatcatt ggtttaatca aagaacccca tcaaatggtg gggtttcttt gattactgtt
     9661 tgatttcacc cattgttact tctatccgat gaattaagcc gtcatcaacc aacggtattg
     9721 acataggatc gatttgtttt ccgtccgatt gaacggtgcg gacgtgccga gagacatggt
     9781 ccgggttccg aacaagaatg aaataggttg actttccgaa acgatattga atttcaaact
     9841 catcccattc cggagggatg acgggatcga tttgcagcga atttccgatt ttatggaagc
     9901 cgagaattgc ctcaattccc aaccggaaca tccaagccgc agaaccggta taccaagtcc
     9961 acccaccccg acgcgtgaaa ggcggttggc tataaatatc ggcacagatg acataaggtt
    10021 ccacacgata aacggttgcc ttttcttcgt tgtccgaatg aaaaatcggg ttgagtaatt
    10081 caaaaaggtg gcctgcttgt tttccgtctc ccagagcggc aaatgcccat gcggtccatg
    10141 tggcggcatg ggtatattgc ccaccgtttt cacgggttcc cggcggatat ccttttatat
    10201 agcccggatc gaccgaagat ttatcaaaag gaggtgtaaa taaaaggatt aatcgatcgt
    10261 cgaagcgaac caaattttcc atcacagaca tcatggcccg ccggcttcgt tcatcatttc
    10321 ccgcgccgct gagaattgcc catgattgag caatggaatc aatttggcat tcctgattta
    10381 aggaggatcc gataggggtt ccttcatcag tatatgccct tcgataccaa gctccgtccc
    10441 atgcgaattt ttcgaccgct gcggcatatt caatcgcttt caatctgtat ttttgccccc
    10501 ggtccggata gccttgtttg tcgcaaattt cggcaaaacg ttgcagaaca tcgcagagga
    10561 accaagccaa ccagacactt tccccctgtc ctttttctcc gactcgattc attccgtcgt
    10621 tccagtcccc ggttccgatc aagggaatac cgtgagaacc cgcggtcatt cctttttcaa
    10681 tcgctctcag acaatgatcc ataatcgaat acgattgctc ggtagatcgg taatcggcat
    10741 aacgttcgag ctctccttcc gcgagcggag gcgcagacaa aaacggcacc ttttcttcca
    10801 aaaggctgtt atcgccggta ctttcgatat atattgcggt gacatagggc agccacagca
    10861 aatcgtccga gaaacgagtt cgaacaccgc ggttgaaagg cggatgccac caatgcaaaa
    10921 cgtcgccttc ttcaaattgc cggctcgctg aaataagaat ctgattgcgc gtaattgtgg
    10981 gatcaatcgg caaaaccgcc aacacgtctt gaagctgatc acggaatccg aacgctccgc
    11041 tggactgata aaaacctgtc cgcccccaga ttctaccgga tagagcctga tacagcatcc
    11101 agtgatttag aatgatatcg gtggcgggtt gaggagtttt aactcggatg gaatgaagca
    11161 aattatccca gaataaatga gtgtgttcaa aagcggcatt gacattgttc ggattatggt
    11221 atttttgcgc caattcaata cagtgttctt tattatttcc ctcacctacg acgaaatata
    11281 tttcttcaac ggcacccggt aaaagatcaa gatgtacctg taaggcagca catggatcat
    11341 cgccgggagt gatatgagtt tcaagtccta acctgcgtag agcagccgga ttggcaaatg
    11401 atcccgctct gcctaaaaac tcagatcggt ccgcggtaag tccgtgaaga tttttactgg
    11461 caatcagaaa tgctacatgg cttccgaatt cggggttatg cgggttcaat gccattaaac
    11521 attcgtgctc cgaatcatat tcgggaataa tgtaagccat atttgcctca tgagtggttc
    11581 ccaaaaccca ttcgacatat tgggtggctg taattcggcg aggatgatcc agcgtgtttt
    11641 ggattttaag atcaataatt tttaccggat cttcaggact ggcgaaaagc gttaagacct
    11701 gatttaatcc atgagaattg tgttcgaaaa cagtatatcc tgctccgtga gtaacccgat
    11761 aaggttggtc cgaaccggct ggaagcggcg tgggtgtcca tatctcgccc gtttcctcgt
    11821 ctctcaggta taagatttcc ccgctcgggt cgataaccgg atcatttgac caaggcgtaa
    11881 ggcgattttc tccgctgttg aaagcccagg tgcattgact gcctgtttcg cttaccatga
    11941 atccgaaatt cggataaccg attacattta cccatggtgc gggagtaaac tttttctgtg
    12001 agaattcagc ggcattccgc tgaagatcgt tccaatcgat gacatactct tttccgccat
    12061 cagcgaaccc accaaaaccg ttgtatctct gcaaatcccc tatttctttc aacggatagg
    12121 tgtatgggat ttggcatgcc aattccggct tttctttgat tctggtggga attaaatcgg
    12181 gaagatgatg ctccggaatt acatattccg gcatctgttc tctgattgaa cctttgtctc
    12241 cgtaaaatat cgcagatgca gcggttctta tgaatgttaa ttcttcttgc gtgacctgat
    12301 caccgtggag aacgaatatc ccggctcgtt ggtttaacca atcttcgctg tttgttttgc
    12361 tgatcgtccg ttgaataacc ccgttcaatt cggctccgta atcagtcggt tgcaaattta
    12421 aaattaccag atccaagtcg aacaaatgcg ttttcaaaaa gcgatatatc tttaatgctt
    12481 cttgaaccaa gtcgatttgt tgggggtcat caacctcaat caaaaggatg ggataatctc
    12541 cggagatacc gaaacgccaa agtgccggtt ggccgagaca attagccgca atgacttcgg
    12601 aaggtgcgcg gaaaatctta ttcggataat tcaatgcgga aagcagatga aggccggttt
    12661 caagcgattg ggatccgatt ttgagctttc ctaaccaggc ttttgctgcg atattggctt
    12721 ggctgaacgc ggtttcgatt gcagcccaat tccgataacg atcggctaaa atcagaagtt
    12781 cttcacggct ttccgcagtg aaagttaaaa acgctaaatc ggtactgcca tgtggcaaaa
    12841 gctctacttc ctgtccgagg gagaatatcg gatcgagtgt cgcgcccgtt gtccccgaaa
    12901 gatattgttc ggatgataaa gcgacgggat ttcttgttga tccgccgcgt ccgataaaat
    12961 gataacgatc cgcttcatgt gagacagttt tttgaatccc tttagtaagg accatatgtc
    13021 ccaaaaagat ggatttttct tgatcggatc gcagtcgccg ggtaaatatc tggagattca
    13081 attccggaat aaattcactt tcaatgaaca ttttattaaa cgccggatgc cggacatccg
    13141 tcaactgcga agacagaatt acttcgccgt aggaagttaa ccgaagagta tggatgtcat
    13201 cctgtgtgtt cattaaatga attcggcgaa tttcaaccgg atcatccgga gctacggtta
    13261 catccatcgt cgaaatgaac ccgttttcgg accttcgaaa aacagccata tgagcataat
    13321 aagtaacctg taaattcgtc ggatcgcccg gtaccggttg ataagtcgcg gaccagaacc
    13381 ggccgggttg gcccgaattt gaatccattt cctgaatata aatccaggtt ccccacgaat
    13441 ccgttacctg atcggctcgc caacgggtta gatcaacggc attcatcgaa ctgtatccgc
    13501 cgcccatatt ggatattaac agatgatagc tgccgtttga caggagattt aattgaggga
    13561 tagtagacag caaaggtact ctccaaggag caatttcttc ggatatcgca accatccttt
    13621 ggagtccttc gacattttcc gcgtccgggt tttgaatggg aacgaaatac gggatttgtt
    13681 cttggagcaa taattcgacg ctttgtattc gtgaatctct gtgtaacctt tgaaccatga
    13741 tgtcattggc gaagaaattt gccattgcca tcatgatcat cccttggtga tgtgccatat
    13801 attcatacac caatgctgac ctttcatctt tcaggagacg atcgggagtg aaatcaatgg
    13861 cttcatacaa tccgaataaa ccaaacattt tatattccaa cagccggacg agattttgaa
    13921 caactgcttc cggttcgtaa gagaccgcca tcaatgaagc ataaggcgca atgaccagat
    13981 catctgccag tccccgctta aaacccaaac ccggaactcc gaaagcccga tattgataat
    14041 tttgacttgc atcgaaccga taaaaaccgg attcggatat tccccacggg actcccttct
    14101 cttttccata ttcgatctgc cggagaacgg ccccttccga actttccgcc aacaaggtag
    14161 ccggataaga atgaaagaat aaagccggca tcaaatactc aaacatggtc ccgctccatg
    14221 ataataagac atataagttt ttgatgcggg taatcggccg accaagctgc atccaatggg
    14281 attgagggac gtctcccttg gctatggcaa taattgaggc aatccgtgct tccgaagcca
    14341 ataaatcata aaaattctgg tctaattgac cggtcacaag atttaatccg atatgaaaaa
    14401 catgtctttg tttgttataa aggaatccaa aatccatctc attcattaat tgctcggatt
    14461 ttgccatgat attttcatat tgtgcgatca ttccgttcgc attgacctcc gcatggtcga
    14521 gaagggtgat caattgattc agccattccc atgcgatatt tctatccgat tcttcagaat
    14581 cctccggcgc ttgcgaagct tcgctttttt cggacaggca atcttttaaa tcctcgatat
    14641 acagagaagc tctctcgatt tggtcatgca attggccaaa tgccaagtcg tagggtaagc
    14701 aactgcgcag actattcatc gcttcggaaa attgaggttg gcggaacagt cggggaggat
    14761 tttcgaagag aggaatccat ggcagcagtt cggtgatcgt ccgttcgatt gcaagattgt
    14821 gctgttcgat ttggcgggaa aattcctgca gtttatttaa cgtttccagg tcaaatgagg
    14881 aaccgccgac agttatcaac tgttgtaacc ggtttgaaat atcttgccaa aaaaatcctt
    14941 ttaccttcag aaaaagtgga taccaatttt cgggatggga tcgtgccgct aaaatttcgt
    15001 tactgatccc tttgataaga gcatctattt ctttgatctg ccggtcgaaa tcctttttac
    15061 ggatagcgtt taatccatca gctaagtttc ccaaggaatc caggtagccc tcccacagat
    15121 tccaacgtaa aattgtttcg tttcttttat tcttacaagc ctgcgatgta atcatcaaac
    15181 atgcagccag atttccactg tcgaccgttg acacataacg gggattcaga ggttgaagag
    15241 taatcgtgtc ataccaatta agaaaatgac cgcgaaatct ttccagttga ctcagggttt
    15301 ccaaagtagt agccaaacgg atcgccaatg tcctttgatc caaataaccg aggtcgtaag
    15361 ctgccaacgt tgaagtgagc aataagccga tattcgtcgg tgaagtgcga tgcgcaacgg
    15421 tcccgatcgg tttttcctga aaatgatcgg ggggcaacca gtgatcttca ggccctacaa
    15481 atcgttcaaa aaagccccaa gttcgtcggg ataccagacg caaaagtttt atttggtccg
    15541 ggttgagctt cgcggatttc ttaataatag gaagattaat ccaccatgca atcatggggg
    15601 aaagaagcca aaggagcaaa acaggagaag cgttaatgat ggccggagcc aaaccgtttc
    15661 cgctgaaccc ccaaataaat tgtaaaagaa tcgtgagtaa caacgatact ccggccactc
    15721 ccgtcatttt ccgccaggaa atatttttcc gctgcagccc gaacaggtgc gcggtttgtg
    15781 cagcggtggt ccattgcaaa agatcttttt tgctgataaa caagcgatag agagtcgtaa
    15841 atatggcatc tgtagaataa tatgcttcat acggcaggaa tgctaccgcc aacaaccaac
    15901 gcaaaaaatt ccaaaacaac ggtcgaaatg ccgctctgac tgtcccccca tgcacaatct
    15961 ggataatgct gttgataaaa cttgttatca acggtatacc cagagaaact aaaacagtca
    16021 aagaccaaag cagagcgaaa cggggaagaa aaataacacc gaggataaaa aataaagaga
    16081 gcgccggcgg gaaaagagag cgacgtaagt tatcaaaaat tttccaccgg tcaatgatcg
    16141 aaaatgtaac gccgaatctt cgaggatgga tcaaccaagg gagcagctgc caatccccgc
    16201 gaatccaacg cctctgacgc aaggcttgaa ggatatagtt ttgcggataa tcctcgatca
    16261 tcgtaatatc cgtgaccaat ccggcgcggc ccattagtcc ttcgaggagg tcatggctca
    16321 gaaccgaatt ttcgggaata tgtccttcca cactgcgggt gaatccatcc acatcataga
    16381 ttcccttgcc gacataaatt ccttcgccga aaagatcttg gtatgcatcc gagacagcca
    16441 gcgtgtatag gtccaatccg gtatctcccg aatagattcg ggtgaaccac gaataattcg
    16501 cactcctggg atgaatttcc atccgcggct gcaatatggt atatccggaa ataacctgac
    16561 ccgtcttttc gtcgaaaatc ggacgattca gcggatgtgc caaagtgcct gctaaccggc
    16621 aagccgcacc gcgaggtaat acggtgtcgg tatccaaggt aatgacaaag cgagttctct
    16681 gcaatattcc tttatccgga ttttcatcgt gaaacccgtc tgtaaaaaca gtgaaggata
    16741 agccggattt tccgcgtagc aacaaattta attcatgtaa tttgccgcgc ttccgttccc
    16801 aaccgatcca ttttccttcg gagggattcc ataatctctt ccggtgaaga aaatagaaac
    16861 ggctgacgct tgtcgaatcg ttttcttccg aattttcggc ggagttctcc gaaaatgaaa
    16921 caggaccgta tttacgattc aaagctccga tcgcttcgat tgtttgtttt accaaatctt
    16981 cgtcttccgg cattgtttcc tgatcggcat ctttaaagtc tgtcaacaag gcaaagaata
    17041 atcccggctc cggattacgg agataatgta attcgagttg ttgaatcaga gattcgatgt
    17101 ctttgcggct cgttattaaa gaagggatca cgaccaaagt ttgaaacgaa taaggaattc
    17161 cgttccgaaa attcatcttg gggagcgtgc gcgggtggac taaaagagta atcagccagt
    17221 ttatcagtcc ggtcgcaacg gtcatagcgg ggacgatccc aactagaata tataagatga
    17281 ttgccagcca ttggactgga cggtagtggg caacagtgcc ggggttaagt gaagaaccgg
    17341 cattaaactg ctgcggaaga ttggttatcg cgcttacaaa acccagaagg aggatggtta
    17401 agaggaaaat cgccgataga taaacaaaat cggcatgttt gaaaatccac ctttggagcc
    17461 gagttttaaa attggggcga tagccgattt tttgttccaa ttgcggtcgc aaattcccaa
    17521 taataatttc gccgatatgt aaccccggat ttttaccgga gcaatcaatc cgatctttat
    17581 ttttatcgat taaagtttca aaataaagcg gcgaatcgga ctgaccttgg ttgatatcgg
    17641 tcgccatgtt caggatggtt tctgcaatct taatttcatc ctgaccggtt gcccgggaaa
    17701 gtttttcgat ttcctttcga taatggtctc gggttttaaa atccatccgc gggtatatcc
    17761 cggacgggtc ctttcgcaat aattcttcaa gaaggcttac ggattcgaag aattcgttcc
    17821 agtcctgttc ggaaatcgta cgtaaactga tgatgatttt ggcaacactg tcattattaa
    17881 cggttctggt aaaggcgccg ttttcctcag aaaattgaca actcgtttcc atattctgcg
    17941 aaaaaacaag cggcaaattc ggttgatcat tcggatgaat gatcgtgacc aactcaattg
    18001 aaagaaactc gatcaggcta taacgcaaaa agataggaaa ggcccacagt tcgcccgttg
    18061 acagcgggac gactttttgg aattgaatca aaatcgattc caattccatc gggtcgatca
    18121 tcagcttttg ataagagagg atcgaacgag cgatgatata tattcggggg aaatctttga
    18181 gaacaccatt cgtaattttc ggcaactgtt gataaaacga aacaggtaaa tcttccttaa
    18241 tctgttgcag tgtttgccga ataatataat aattatccaa aatccactcg gatgcatagg
    18301 ttagaggaat tttattttgg gaggattcac tgaaataatg atgagcttca tccaaccaag
    18361 cctcaaattg cgatagattt tttcccggaa tatcccttcc cggtaaagcg gattgagctt
    18421 tacgggtcgg tttttgaaat tggacttgct gacaaacatc tgtaagcgaa gtcactaatt
    18481 cctgaatttg acgtgatttt aattcagagg gattcaggtt ggaaaaatcg ttctgatccc
    18541 tctcttgctt tttttgattt tcttcaacca ttaacgacca ccgacttttt taaatgcaat
    18601 ctccgctgcg gtaggttgaa attcagaacg gggaacatcc tggataatta aaacaggctt
    18661 ggtaccgtat tcaatataat tacgtgcgat actgccatac ggccattcaa tttggccggt
    18721 ataaccatgt gcacataata cgaccagatc aatgttttct tcttgattcg caaagttttg
    18781 aatcgcggaa gaaacactgg aactttcgag aacatatgtt tcgcaatcta cctgcagacg
    18841 ggcttttgtt tcttcaagat actcactgac tactcgacga ctgactgtca ttaatttttt
    18901 ggtaagctgt tcgatttcgg gcggataggg ttccggaatc ggaatctcgg gaggctttat
    18961 caccgcgatg agcacgagct gcgtaggaag tgacggaatg gtctttgccg tctgaatcgg
    19021 cttgcggctg tttaattcgg aagcgaatcc ttccggtatt tctccgcggg ccagtgccag
    19081 gcctaccgag agcgcacatt cagcccttct ggagctgtca accggcaaca gtattcggcg
    19141 ataatggaca cgattcacca ccaatttcgg ttgataatac gaacgaatca gaagaatcgg
    19201 taaataaatc atatcgatta ctttttgagt gatactgctg atattccaac ggcttaatcc
    19261 gcctgctccg tgagtgctga tgatcaataa atcgatattt tcctgatgag caaaatttac
    19321 aatgctctcc gcggtttttc cttccgaaat ggcatattcc actcgcgatt tgggggattt
    19381 cgtctccggg ttttcaaaat caaccgctgg catgggatcc tcgagcaaat ccgcattcat
    19441 tctttttgta acttcctcta aataaatttc agccttcttt ttccgaatct gccagttcag
    19501 cggatcgaca gatatgggat tttcacgaaa ggaagcagga tcgagaactt gtaaaagaat
    19561 aattttcgat ccgaaaatgc gggcaaattg ctccgcatga taaattgcct gctccgcaag
    19621 tgctgatcca tccaatggga caaggattct cttgaacatg atgatccttt ctttttatga
    19681 ctattttaac tgaatcaatc caataaaacg aaataatcct ctctcaaaaa gatctatttg
    19741 agtttgatga aatattctat atagtaatag tatcacagga agaaagggct gatgttatag
    19801 gaagttttcc aagtagtccg gcgttgactt tgcattcaag tccgagaaat aataaaacag
    19861 aaaatgattt ttttgtgacc catctgggac tcgaacccag aacacgttga ttaagagtca
    19921 actgctctgc caaattgagc taatgggccg gacttccttc aagcaatgca ggattatacc
    19981 ataagttcga aggatcaaaa aaattctacg atgtggtgtt gtccgcggga atccctctgc
    20041 gttctttgat caaaccttgt tgataggctg acattaaatt tttcagatcc tcgattttat
    20101 gaattaagtc ttttccgccg tcggtatcgg ccacagtcaa acggttatcc gaagtttcaa
    20161 aaacttgttc ctgcgaaaat atgtcgctgt tattttcaat ggcattttta atgcctaaaa
    20221 aaggttcatg tgacgaaatt cgcatcccat aagaatcgta aatgagcgta tagcctgcga
    20281 tgcccgtttt cttttgatag gctttgcaaa aaccgccgtc gattacgatt attcgtccgt
    20341 tggctttaat cggtgattca cccatgattg cattgaccgg tacatgtccg ttgatgatat
    20401 gactggtctc ataatccaaa ccgaattctt caaatatctt acggcagatg accggatctt
    20461 cgattaattt gtaatacgcg ttatcttctt caatccaagc ttccggatca tcgacatata
    20521 aacgttcaaa ggttgttata cgatctctgc cgaaaagtgg cgaatgtctg ccgcgccata
    20581 agaaccaaag gaaatcccgt ccgtctttct tttctttact cccttcggga gcataatacg
    20641 cttgcctggc cattttatcc gcgtagtcga aatatgattt tccgtgataa ttcacatcgt
    20701 tgacgcagaa attcataaaa gttccgtccg gattcatagg aatacacccg tgaaacagaa
    20761 gattattgtt gcattgcaga tacatggatc ccgcggaata taagaatcgg atatgctgtt
    20821 gcaagcgata gctgttttta aatgtcgtaa acagttgatc taaaaccgat tgctcatcgg
    20881 aagttaattg ataaggattt tcttcatcca tcgggaacag atcattgatt ttgatcggat
    20941 acgtttttcc ggcgatccaa attgtccctt tctttcgatt tatatgattt aacaacagac
    21001 aatcatccat gttgtattcc ggatgtctga ggatgatctg accttccaat ttgaataaaa
    21061 taaccgaaat tgcttcatac attttggata aaagccgtaa gtcttcattc ccgatttcat
    21121 cgttgctttt caattcatag cgttttgatc ctttatattg ctgctgagca aataatgcca
    21181 gtgggcgcag attgatgccg taagctgtct ccaaggtatc caaatttccg tatttgattg
    21241 aattactcag aacagttgcg atacaggcgg cggaaccgga tgcggctccc atccataaaa
    21301 catcatgatt tccccactga atatcgacgg agtggtgggc acgcaacgaa tcaataatga
    21361 tatccggccg cggtccgcga tcaaaaatat cgccaacgat atggagctga tccacgataa
    21421 gacgttttat aacggtcgaa aaagcgacaa tgaaatcttc cgctttaccg atttcgataa
    21481 tggtcgaaat aatactttca tagtattctt cgcgggatgt attttcggcg ttgatcagtt
    21541 cgttaataat aaaggcatat tccggtggca gagatttacg gactttgttg cgtgaatatt
    21601 taatcgatac caaatgggca atttcaatca gctgatgaat cgtgatcata taccattcat
    21661 taatattgat ctcttgattg ttttttattt ccttgagctt ctcttcggga taataaatca
    21721 gtgtggaaag ttgggctctt tccgccgagg tcagcatttt cccatagaga gaatccactt
    21781 tttttcgaat ttctcccgat gcgttattca ttatatgaat aaatgcttca tattctccgt
    21841 gcaggtcact cataaaatgt tccgttccct tgggcaattg gagcagtgcc tggtagttga
    21901 tgatttcagt cgaggcttct tgtatattcg gatatttttc agccaacaaa tccaaatatt
    21961 tcagatccaa ggtcggttcc tcagtgtatt tttccatttt tgattatcct gtttcttccg
    22021 atggtcaatt tgatgtacgt aagtgtattg taatctctga tttcttattt ctcaaacagc
    22081 aaaaagcgga atgcaaacac attttggcgg atgaatcaat atcatcttcg aaaataaatc
    22141 tcttttttgg gggtattcga ttgaaaatac agacactcca tgaaaagaat ggggcgatgt
    22201 aaagcaaccg gaaccgaccc tatttgttcc gggtgattca atcgtttttc ctttgatgat
    22261 ccgttgcggg aatttattgt tttagaaaac ccttatcaaa catagagcaa atcgtcggaa
    22321 aagaaatatt ctttcagagc ggtgatggcc ccggcgactt caagttttgg gagaggctgc
    22381 ggatcggaaa cgattcggaa ataacgatcc ggagtcagta aagaaaaatc ggaaagatag
    22441 ttatataatt ccgtttcaaa agcatcatta aaaccggaaa agaaaccttc aatgatgaaa
    22501 acatcagggt catataccag cgagatattc agaaaagctt gtccgaaaca agctgccaat
    22561 gtttttaatt cgctttggca gcaagcaaat cctttttcga aacccttcac gacatcatca
    22621 aacataattt ctttcaacgg tttttgaaca aaggaaagaa aagaatcaag atcttccccc
    22681 tgctttaatc gagaacggat cgattcgggt ttcacaatac tttcgaagca accatgtttc
    22741 ccgcatatac aacttacggg ataatcgggc ttcaaaatca tatgtccgaa ttctccgatg
    22801 attgaattac tgccttcaat catctgaccg tgatcaaaaa agcagccgtt gatccctttt
    22861 gcggtgtaaa cggtcacgac gcgtttggtg gaacaaaatt cgaggttagc ggttatgga
//
LOCUS       DAOSMM010000033        22662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2288_length_22662_cov_16.134825, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000033 DAOSMM010000000
VERSION     DAOSMM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 22662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22662
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2288_length_22662_cov_16.134825"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PK898_06165"
     CDS             <1..1142
                     /locus_tag="PK898_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOU19654.1"
                     /translation="PSMHLPWHYLPVWIFISTPFFYTALFFIGFVYLIKPFHIRDLDQ
                     VFDFFILGILIISFLLPICLHSTLYNGWRHFYFLYILFLYFVIFALSILLENKTKAIR
                     SATLGLSVGSLIFNGAWMIRNHPYEMVYFNALIREKAPYNFEKDYWRASSSECLRFIA
                     SQSADLRIEVGDYDAELYSAKFALKKSDRDRIVEEPFGYDASHMKYLVLNYTKVIGNE
                     KEIPFYEPVYKIQVDGMNIAAVFERKDRDGIWANAIVKDVTSNVNPQLTPLIFDPDDQ
                     TGWSTERFQQAGDYLIFKLNDTMKLYGISILSGTGKYEYPQDLQIEGSVNGVDWEILP
                     LTYHTQADYTFTPAEVRYIKLMLDQDSDENAWTVNNVMFHVVPNQ"
     gene            2294..3337
                     /locus_tag="PK898_06170"
     CDS             2294..3337
                     /locus_tag="PK898_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOU19655.1"
                     /translation="MIKQFQIKNSDQIFDYIVLGIFIISILLPICLHSTLYDGWRHFY
                     FLYGPFLYLAVLGLSALLAIKNIILKSVILGISVGSLIYNGAWMIRNHPYQMVYFNVL
                     VRDKAPDNFERDYWGSSSSECLRFIVSQTDDLRIHVGDYDAGLLAAKFSLKKSDRERI
                     VDDSFGYDANRMKYLILNYTRVIGNEKKILFYEPVYKIQSDGMNIATVFERKDRDGVW
                     ANAIVKDVTSNVNPQLTSLIFDPDDQTGWSTERFQQKGDYLLFELKETTKLYGISILT
                     STERYETPQELRIEGSENGTDWVTLPLTYQTHSDYAFSPVNIKYIKLILEQNSDEIAW
                     TVNDVMFHLASNE"
     gene            3558..5342
                     /locus_tag="PK898_06175"
     CDS             3558..5342
                     /locus_tag="PK898_06175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOU19656.1"
                     /translation="MRTKKRKFSLKWGLIIIVIACWVVPILVIIGTLGSFTMRNIESQ
                     IADTITTNAENMVTLSQNMIENAIKSSRAASYDRDIWNAYSLYRQDHDEISLYSSVTS
                     YLVSKYRDNNQFISTFLYFCDEPDKVYYANNRASSGSFTKVREYRSNMHSIVREAASK
                     SGTEIIFLSDGEKLYMVRNMVDSKFQPFAVIIMELNRDVMFDSVRSVVWIEGASVYFN
                     SIRSDVIGEPVSLPEYEINLNQPILEKKDGRYDIFIRKKVEGQTISYAITTNQSLLSD
                     DIPNFRRMIWLLILFTVPLMALVIWIFYRFISKPISAMIEASQKIESGEIGYQIQPLP
                     DSNEFNYLTERFNSMSHQLKVQFERSYQEQIALQDARIKALQSQIDPHFLNNTLETIN
                     WEARISGDEKVSSMIEALSTMLSAATARGGETMISLAEELTYVNAYLYIISVRFGKRL
                     TVKQEIDEALLDEKVPRLVMQPIVENAIEHGIAPHRNGDLILRIYRQEEKIVMEVENS
                     GELTDADKEAVDRLLNWDGNDTTDIKPGRLGIRNVNLRLKFLYGKECGLTITSLPNQR
                     TLARIILLNQSNSSVSLYNPDNQTKLYK"
     gene            5441..6700
                     /locus_tag="PK898_06180"
     CDS             5441..6700
                     /locus_tag="PK898_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19657.1"
                     /translation="MKKRSIFIVGLVIIFSLMIASVAFAEEITVVTSYGGDDGNRGNY
                     EAAVKMYEEQTGNKVNDESGTSNEEWKAKIMTDFETGAEPDVLFFFNGVDSNPFVKAG
                     KVVSIDEIREVYPDYASNMKDSMLGASPVDGKNYSVPVNGYWEGLFVNKKVLDEAGVA
                     VPGADYTWEQFLNDCEKIKTAGFTCVAVSLQEVPHYWFEYTVFNHGSVANHLDIPKTA
                     DDAVAQKWAAGLEDIKVLYEKGYLPANTLTATDAETFQLMADHKAAFAIDGSWKIGWF
                     QSNADDINNFTVTYVPAQGDRAATDIIGGLSMGYYITRKAWNDPEKQKACVDFVGMMT
                     SDDVVSTFGATAVTALKKGTTPPQDADALVTAALAMTKGATGISPAVQDLLTGEARAD
                     LFANVKNVVTGKITATDAIASMISFNQ"
     gene            6803..7693
                     /locus_tag="PK898_06185"
     CDS             6803..7693
                     /locus_tag="PK898_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU19658.1"
                     /translation="MSSTIYKNKLPLIIFLVPALILMTIFLYYPFIMNIFNSFSDISG
                     LGTAAKGLNDPWFKNYIRLFQDPNMITAVKNTMILMLSTIIFQVGIALLLAIFVDSIK
                     VGSQFFRTVYFFPLVISATALGLLFNLVFLYDGGMLNQILQKFFGFTQNIDWKDENHF
                     MLTMLSPVMWQYVGFYFVIIVTGLSNIPQEIFEAASLDGAGFWKRTYYISLPMIRNVL
                     ITCLILAITGALKVFDLPWVMMGSGIPMDESWLTGTYMYNQTFLRSDVDYGSTIAVVI
                     VILGVVISQIANKIFKEKEY"
     gene            7695..8582
                     /locus_tag="PK898_06190"
     CDS             7695..8582
                     /locus_tag="PK898_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU19659.1"
                     /translation="MGNKRFSSKRIHSYSFTTLFSYTVLTIWAITTIYPFVWVLINSF
                     RVKGQIRSDSFSIPWPWLDSFTLDNYSTAFERSNVFRAYGNSLIISIVVTLFVSLFAG
                     FAAYGLVRYSFKGKEILHSFIIASMMFPVFSTIIPVYRMEVHWGIASTGKLPLSLLST
                     ILPQIAGNLSFAIVVLMGFIQSIPIDLEESAFIDGCNVFQIYFRIILPVAKPSFATVA
                     IFTFLWSYNDLFTQMFFLRRPKERTITLLLNEINSQAGVNYGLMAAAVVIVVVPVLIV
                     YVLLQKNIIKGMVAGAIKG"
     gene            8622..9371
                     /locus_tag="PK898_06195"
     CDS             8622..9371
                     /locus_tag="PK898_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU19660.1"
                     /translation="MYKVVIVDDEEIIVRGLQSVVNWNSYKCEVVATAFDGISGAIAV
                     RTYLPDILITDIKMPNRDGLTMLAGLRSEFPAMQVTILTGYADFDFAQKAIKLGVSRL
                     LLKPSRMSEINEALQFMTDTLAKRSPAASDDKIDDLSTASANSFIVRQALAYIEENFS
                     RKLTLQDVADYCYVSQWHLSKLLNKVTGQSFYDLLNKARIDAAKRLLEDPKLRISDIS
                     EMVGYTDNGHFSRLFKKMVGISANDYRNQVK"
     gene            complement(9403..10836)
                     /locus_tag="PK898_06200"
     CDS             complement(9403..10836)
                     /locus_tag="PK898_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOU19661.1"
                     /translation="MIHFGTGGWRAIIGDEFTKSNIRLLSYSIGDLMEKTGTANLGLC
                     IGYDRRFLSKEAAHWAAEVFAARQIPCYVIDRSSPTPLIMFTVKQMNLPYGMTITASH
                     NPAIYNGVKFFTAGGCDANEDVTNEIEFLTQKNAEVKIPFLDYDEGIRSGIIKEINPL
                     NSYIDSILSLVDTEAIKKARLRIALDPMYGVSQTSLQTILLTARCEVEVIHDRHDTLF
                     GGRLPAPNIHTLGTLVNFVLDHHCDLGIATDGDADRLGVIDDKGQFVHPNKLLVLLYY
                     YLLKYRGWRGPAVRNNSTTHLLDKVAAGFGQACYEVPVGFKYVSAKMEETGAIIGGES
                     SGGLTVKGHIRGKDGIYAATILTEMVAVTGKHLSELYKEITDQFGEVFNSDADFKLTP
                     QNKERLQNILYVENKLPDFPYEIDHVSYRDGCKVYFKNSGWVIVRFSGTEPLIRLCAE
                     MSDQKSADQLISVFSDFLGLESELQSQ"
     gene            complement(10833..13235)
                     /locus_tag="PK898_06205"
     CDS             complement(10833..13235)
                     /locus_tag="PK898_06205"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19662.1"
                     /translation="MRFGFFDDAKREYVVEQVDVPVSWTNYIGTKNMCGVFNHTAGGY
                     LFCGSPEYHRITRFRPNGVPMDFPGHYVYIRDDDTGEFWTISWQPVGLPLDKAKYQCR
                     HGLSYSVYSCEYREISASQKLFVAIDDPLEIWDVNIQNQSDRRRHLSVFSYLEFSFHH
                     IAMDNQNFQMSLYAAGSRYQDGIIEYDLFYEEDGYQFFTADFEPDGFDCVRDRFIGPY
                     RTEKNPIAVESGHCSSSFEKGGNHCGTLSKQLSIDPGEKIRLIFLLGEGSLEEGRKIR
                     KKYADPHEVDRQFDLLRTFWDEKCSKLQIQTPDDGMNSLINIWTLYQSEINVTFSRFS
                     SFIEVGGRTGLGYRDSAQDAMSIPHSDPVKCKSRIVELLRALTSEGYGLHLFDPAWFE
                     PQQAKKPFKSPTIIPVPEKSSMIHSVDQACADDALWLIGAIHNYLKETGDFDFVWEEI
                     TYADKGSGSVYDHMKRILDFSEKQVGKHGICKGLRADWNDCLNLGGGESALVSFLHYW
                     ALTQFSELADFLGEEKDAEHYRHVAEHVRQVCEEVLWDDGWYIRGITADGRKIGCHQD
                     VEGKVHLESNAWAVLSGVASQERGEQCMNAVEEYLFTPYGLMLNAPPYTKPDDGIGFV
                     TRVYPGLKENGSIFSHPNPWAWAAECTLGHGDRAMRYYNALSPYKQNDQIEIRQAEPY
                     SYCQFIVGKAHTAFGRARHPFMTGTAGWAYYAATQYILGIKPGFTTLSIDPCIPESWD
                     GFRVKRIWRGAEYDITVENPDNVQKGVKALYCDGLPVAEIPLKPCGTSTNVRVVMGKQ
                     TI"
     gene            13457..14779
                     /gene="ffh"
                     /locus_tag="PK898_06210"
     CDS             13457..14779
                     /gene="ffh"
                     /locus_tag="PK898_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU19663.1"
                     /translation="MFENLTERLNHIFAGLTRRGKLSAEDVDAAMREVRIALLEADVH
                     YSVVKTFVANVRERAIGTEVSEALNPGQMVIKIVNEELIKTLGPAEKLNLSGEKPHVI
                     MLVGLQGSGKTTQTAKLAKKLREQGERVLMVAADPYRPAAIQQLQTLGERINVEVFTK
                     EGIKPPELSRQALEYAKKGGFGIVLIDTAGRSQLDNVLMEELQAIQKITHPNEILLVV
                     DSMIGQEALHVAEGFRDAVQISGLILSKMDGDSRGGAAISVRSVTGVPIKYLGTGEGL
                     DAIEVYDPSRLSSRILGMGDIIGLIEKAESVYNEKQAKQQANRMLSGKFTLEDFATQL
                     RQVKKMGPVQQILGMLPGSLGQAAKQIDPKEAEKSLKVTEAILSSMTLKERENPDILN
                     ASRKRRIASGSGTDVQEVNRLLRQFRESQKMMKTMQKTGGRGLSNLFR"
     gene            14898..15254
                     /gene="rpsP"
                     /locus_tag="PK898_06215"
     CDS             14898..15254
                     /gene="rpsP"
                     /locus_tag="PK898_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOU19664.1"
                     /translation="MVRIRLRRNGLKNQSSYRIVAADKESPRNGRFLEILGSYNPRTE
                     PFTFQVKEDRIYYWMSNGAQPSESVVKLFKSVGLMDRFERYKKGESIDALMEEANNYY
                     AQRKVNVKTREAALKS"
     gene            15254..15550
                     /locus_tag="PK898_06220"
     CDS             15254..15550
                     /locus_tag="PK898_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOU19665.1"
                     /translation="MQHKEDVKMQRNSSNKGDFAKDLVEYIALSLVEDTNSVRVTQER
                     SGNRVRLELHVAKEDMGRIIGKGGRVANSIRLLLRVASARDGRQISLDVIEPDS"
     gene            15567..16127
                     /gene="rimM"
                     /locus_tag="PK898_06225"
     CDS             15567..16127
                     /gene="rimM"
                     /locus_tag="PK898_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOU19666.1"
                     /translation="MKAKSHRDQQEFNQQGSSNIDEPEFICIGKIHRSHGINGAVIMD
                     PMTDFPERIRSGRKVFVGTSRAPMVIKSARSMPPFFLVQFVEIDSPEKASELRNQFVF
                     VKRIDLPKLPKGRYYFHQLIGLEVFEINGTRIGKVKEILETGANDVYVIEKEDQTEVL
                     AAGIPEVIREINLEEKKMVVNLPEWI"
     gene            16134..17237
                     /gene="dprA"
                     /locus_tag="PK898_06230"
     CDS             16134..17237
                     /gene="dprA"
                     /locus_tag="PK898_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU19667.1"
                     /translation="MPDQLAYWVAFNHIKGFGSVRFQLLLNYFSSLEAAWKATPIELA
                     AAGVHERQIKAILDFREKNSPEDLLNKIQRSGISVVTRMDDNYPALLKLIATPPPVLY
                     FKGTLPEQDKKCIAIVGTRRMTAYGSEMAREISAFLASRGVVIVSGLARGIDSVAHQA
                     ALNCGGETVAVLGSGVDVIYPPEHRLLAKNITESGALISDYPPGTAPERTNFPPRNRI
                     ISGMCSACIIIEAGEKSGSLITARFAAEQGREVFVLPGNLNAPQSIGTNRLIRDGARP
                     LLQKEELLEFIESYESVNPVALNKKIQISFEDPLEQKILDLIDVEALHVDEISRRLGL
                     TAGKASSILTMLELKGFVQETAPQTYRKYLSLF"
     gene            17375..18460
                     /locus_tag="PK898_06235"
     CDS             17375..18460
                     /locus_tag="PK898_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU19668.1"
                     /translation="MENDFYAVIMAGGGGTRLWPLSTKDHPKQMLKFAKDETMFQMTV
                     SRLTGKIPFENILVVTTEDQAAKLKQQCNDIPEENYLIEPLPRGTASVIGLAAIHLEK
                     RMPGSVMAVIPSDHFVLNEKQFLALLENGYRAAKDQHLVTLGVKPSFPSTGMGYIEQG
                     EKIPVGYDLYHVGQFREKPDKETAEYFVQSGKFWWNAGIFVWSSRRILNEIAMFLPDL
                     SKKLDIINATIDRPDYTDTMEKTWRLIEPATIDYGVLEKTTDVVFLPTYDLGWNDIGS
                     WESLFDVIPPDENGNIIINCKFHSIDSDGVLACSENKEKMIVSIGVKNMIIIETEKAV
                     LVCPRSETQRVREIVDFLKKNNLTAYL"
     gene            18470..>20456
                     /gene="topA"
                     /locus_tag="PK898_06240"
     CDS             18470..>20456
                     /gene="topA"
                     /locus_tag="PK898_06240"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU19669.1"
                     /translation="MEAYCLKCKQKREIQNPIAEYTANGRPITKGTCPVCNSKLSLIG
                     KTAAHEGLEKPQITKAAKKANSKKVVAKTHSKDPNPKKLNGSKNGTNTTRGIGQKLVI
                     VESPAKARTVGNFLGKGYIVKASIGHVRDLKKSTLSVDVENGFTPEYRVPNEKRALVK
                     ELSTLAKNSDEVYLATDPDREGEAIAWHLLEAADIDPKKTERVVFHEITKPAIAEAFK
                     NPRDINMDLVDAQQARRILDRLVGYNLSPLLWNKVQRRLSAGRVQSVAVKLIVDRERE
                     IENFVPREYWTITVELQPQDIKGTFLSRLVKIDQADPELNNEEQVQFHLNHLQSADYV
                     IDAIKKNVRHRKPSAPFTTSTLQQEASKRLGLTANRTMALAQQLYEGIDLGSEGSAGL
                     ITYMRTDSTNISEIAQNEARKYIQQTYGSGFVPKTAPVYKTKTQRAQEAHEAIRPTTV
                     NRTPEQMKPYLNRDLFRLYQLIWQRFVSSQMEDALYDTLTIDVSAKDAHDYLFRSSGS
                     KLKFQGFLAVYEESKDEDLVIDEMENTKFPEGLAVGQKLQCIHTIPEQHFTQPPARYT
                     EATLVKTLEENGIGRPSTYASILSTIQTRGYVVREAKRLYPTETGILVNDLLVNSFPN
                     VVNIGFTSQMEGELDDVAGGEKKWQTVVGDFYKEFEPA"
     assembly_gap    20457..20556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20557..21009
                     /locus_tag="PK898_06245"
     CDS             <20557..21009
                     /locus_tag="PK898_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HOU19670.1"
                     /translation="EKKWQTVVGDFYKEFEPALNQARKELPKAQLEPEKVGRKCPQCG
                     KDLVIRNGKYGKFISCSGFPQCRYTEQIVEKLDVPCPKCGKELIVRRSKNGRIFYGCS
                     GYPECQFTSWKKPVKQKCPNCGGMLVEMNRDTLQCIECKTKVENKDSE"
     gene            21110..>22662
                     /locus_tag="PK898_06250"
     CDS             21110..>22662
                     /locus_tag="PK898_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19671.1"
                     /translation="MKEKAKAFVEQNPFWTGIIAGFLICFALIGLLSGIGAEQTNSFK
                     NADQILKEDYLRMTVEDYAFTKNADRAKWRYKQLGEEAPSLIALLKKDDLTDPLALFE
                     FSRVVGDMTLFYEGSEIVPEQTNQPVSQEESGGVSLIGKIFILLILLILVGAIVLFLV
                     SNKSSSVKEKTSRFFARFQNKKEPSWKVKTDDQFDLGKEIKGHPIGSRETKEDEDLYK
                     KADRILTNEFELSDAWVEDETGEENDEINSSDTSETLIQTKPRSDAPEESDVSDEDGF
                     KNVNITQKEGEESPETEENLGNSEDHLSGSEFFDEDGFDSELESFEDSQPEDAISEKP
                     DEEVQIPDTEPDIHPEEKNIGSDFSFKPESGRKNETSETISKEDLSSEDEPLIHYQTI
                     YKLGDDFFDETFSLDEGDKFIGECGIGIAETINNTDPKAVTAFEIWLFDREDSQTPTH
                     FLLSDYAYNNDELLERLKNKGRFDLIERNRDYEVKTQNLRMIVTIKDMEYGTESSDKK
                     SYFNKVTFDV"
BASE COUNT         6797 a         4334 c         4931 g         6500 t
ORIGIN      
        1 aaccaagcat gcatttgcca tggcattatc ttccggtttg gatttttatt tcgactccgt
       61 ttttttatac tgcattattt tttataggtt ttgtttattt gattaaacct tttcatataa
      121 gagatctgga ccaagtattc gattttttta ttttaggaat tttgattatc tcattcctat
      181 tgcctatttg tctccattcg accctttata atggttggag gcatttttat tttctctata
      241 ttcttttctt gtatttcgtc atttttgcat tatcaatatt attagaaaat aagaccaaag
      301 ctattcgatc ggcaacatta ggattgtcgg tcgggtcact catctttaac ggtgcctgga
      361 tgatcagaaa tcatccctat gagatggttt atttcaacgc gcttattcgg gaaaaagcac
      421 cgtataattt tgaaaaggat tattggagag cttcctcatc cgagtgcctt cgatttattg
      481 cttctcaaag tgcggattta cgaattgaag tgggagatta tgatgccgaa ttgtattccg
      541 caaaatttgc tttaaagaag tcggatcgcg atcggattgt cgaagaacct ttcgggtatg
      601 atgcgtcaca tatgaaatat ttggttttga actatacaaa agttattggg aatgaaaaag
      661 agattccgtt ttacgagccg gtttataaaa ttcaagtaga tggaatgaat atagctgcgg
      721 tttttgaacg gaaagatcga gacggaattt gggcgaatgc aattgtaaag gatgtgacat
      781 cgaatgtgaa tccgcaatta actccgttaa tatttgatcc agatgaccaa acaggttggt
      841 cgacagaacg atttcagcaa gcaggtgatt atttaatatt caaattgaac gatacaatga
      901 aactatatgg gatatccatt ctttccggaa ccggcaaata cgaatatccg caagacttgc
      961 aaattgaagg atctgtaaac ggggttgatt gggaaatcct tccgttaact taccatactc
     1021 aagcggatta tacatttacg cccgctgagg ttcgatatat taaattgatg cttgatcaag
     1081 atagtgatga aaatgcctgg acggtgaata acgtgatgtt ccacgtcgtt ccgaatcaat
     1141 aattttatac agattgatct cggaaattta tctttcggat atgtcggaga aaaggaatca
     1201 atttgattca gaaaatcgat aaatagatat taggctaaat atataaatga cttctaataa
     1261 gactacacat cacgcgattc aatcggttga aatgaataat gttatatatt agatcgaaat
     1321 tataaataaa ttttacatat agggaaaata aatgctcaaa gcgcaaaatc gatcggataa
     1381 aattttaaag tggattacct tttttgtttt tattattttt tttgtaacgg ggctatttat
     1441 tttcgatgac tatggtgtaa cggtcgatga accgattgaa agacagaccg gtttagttaa
     1501 tctaaaattt atcttacaaa atctattcca aaaacctctt ccgaatgaac tatcaacagt
     1561 gccggatttg cagaattatg aagataaaga ttatggagta attttacaat ttccgacagt
     1621 cctatttgag tattttacaa atttccgcta tgatatcagt tcaattttca aattaagaca
     1681 tttatggact ttcttgaatt tttatatttc cgtgctattt ttcaatcttc ttttgtatca
     1741 gcgatttcgt aatcgtctca tttcactttt gggaacatgc tttctgatta tttctccaag
     1801 gatttttgca gacgcttttt acaatatcaa ggatttaatg tttctttcat ggtttatcat
     1861 tgggttattt tttctatatc ggtttatcca tcatccaacg aaacggaaca gcctgatatt
     1921 ggcgatcgta attgctttgg cttcaaatat tcggattgtc ggatttattc tgttaattgt
     1981 tacttgtatt tatttaattt atgcagtcct aataaaagaa attagtaaaa aagaaatctt
     2041 tataaatggt atgatattcg tcggaataaa cattctttta ttctttttac ttcttcctgc
     2101 atcctgggga aatccgatag attttttgat caaatcactt caccattttt caaaaaatta
     2161 ttatgagagt caggagctgt atttaggaaa atatgtgccc agtatgcatt tgccatggca
     2221 ttatcttccg gtttggattt ttatttcaac cccaatagta tatatagttt tatttctcat
     2281 aggtgtattt tatttgatca agcaattcca aatcaaaaat tctgaccaaa tttttgatta
     2341 tatcgttttg ggaattttta taatctcaat cttattaccc atctgtctac attcgaccct
     2401 ctatgacggt tggaggcatt tctattttct ttatggtcct ttcctttatt tagctgtttt
     2461 agggttatca gctttactcg caattaagaa cataattctg aaatcggtca ttttaggaat
     2521 atcggtcggt tcattgatct ataacggtgc ttggatgatc aggaatcatc cctaccagat
     2581 ggtctatttc aatgtccttg tgagagataa agcgcctgat aattttgaaa gagattattg
     2641 gggatcttcc tcatccgaat gtcttcgttt tatcgtttcc caaaccgacg atttacgtat
     2701 acacgtcgga gattatgatg cggggttatt agccgcaaaa ttctccttaa agaaatcgga
     2761 tcgagaaagg atagtggatg attcatttgg ttatgatgcg aatcggatga agtatttaat
     2821 tttgaactat acaagagtca tcggaaatga aaaaaagatc ttgttttatg agcctgttta
     2881 taagattcaa tcggacggga tgaacatagc caccgttttc gaacggaaag atcgagacgg
     2941 tgtttgggcg aatgcgattg ttaaagatgt gacatcgaat gtgaatccgc aattaacctc
     3001 gttgatattt gatccggatg atcagacagg ttggtcgacg gaaagatttc aacaaaaggg
     3061 agattattta cttttcgaat tgaaagaaac aacgaaatta tatgggatat cgattcttac
     3121 aagtaccgaa aggtatgaaa ctccgcaaga attaaggatc gaaggatccg agaatggaac
     3181 agattgggta acactgccgc tgacgtatca aacacattcg gattatgcgt tctcaccggt
     3241 aaacataaaa tatatcaaac tgattttaga gcaaaatagt gatgaaattg cctggacggt
     3301 aaatgacgtg atgtttcatt tagcttcaaa tgaatgatgt agatgaattc ataaaaagaa
     3361 aaattctttt tattttaggc aggagaatcg acaaataaat ccgttccgga tttcgatcat
     3421 cggttaatag aatttcagtg atattcactg ttttttttca gattaactct gcagatcagt
     3481 ttataaaaat gttcatatca gaattctatt ttagagatca tgaaccgatt ttctcattct
     3541 cgggatacaa tccaaatatg cgaacaaaga aaagaaagtt ttcactaaaa tggggattaa
     3601 tcattattgt tattgcttgc tgggttgttc ccattctggt gattatcgga acattgggat
     3661 ccttcacaat gagaaatatc gaatcgcaga ttgccgatac gattactacg aatgccgaaa
     3721 atatggtaac gctttctcaa aatatgattg aaaatgcgat taaatcctcc cgagcagcat
     3781 cttatgatag agatatttgg aatgcctatt cattatatcg acaggatcat gatgaaattt
     3841 ctctttattc atcggtaacc tcatatttgg tatcgaaata tcgcgacaat aaccaattca
     3901 tttcaacttt tttatatttt tgcgacgagc ccgataaagt ctattatgca aataatcgag
     3961 catcttccgg ttcttttacc aaagtcaggg agtatcgcag caatatgcat tcgattgttc
     4021 gggaagccgc ttcaaaaagc ggaaccgaaa tcatattttt aagcgacggt gaaaagctgt
     4081 atatggtacg caatatggtg gatagcaagt tccaaccttt tgcggtaatt attatggaat
     4141 taaatcgaga cgtaatgttt gacagtgtca gaagcgtggt ttggattgaa ggagcttcgg
     4201 tttattttaa ctcgattcgt tccgatgtga ttggagagcc ggtttctctc ccggaatatg
     4261 agatcaattt gaatcagccg attttagaaa aaaaagacgg aagatatgac atttttattc
     4321 gcaagaaagt ggaaggacag acaatttcct atgccattac cacaaatcaa tctttacttt
     4381 ccgatgatat tcccaatttt cggcgaatga tatggctgct tattttgttt actgtaccct
     4441 tgatggcttt ggttatttgg attttctatc gatttatttc aaaaccaatt tcggcgatga
     4501 tcgaggcatc ccaaaaaatt gaatccggag aaatcggata tcaaattcaa ccgttaccgg
     4561 attcgaatga gtttaactat ttaaccgaac ggttcaacag catgtctcat caattaaaag
     4621 ttcaatttga acgaagttat caggaacaga tagcacttca agatgccaga attaaagctt
     4681 tgcaatcaca aattgatcct cattttctca acaatacgtt ggagaccatt aactgggaag
     4741 cacgaatttc cggagatgaa aaagtatcca gtatgatcga ggctttatcg accatgcttt
     4801 cggcagccac tgcgcgcggt ggggaaacaa tgatttcgct tgcagaggaa ctgacctatg
     4861 tgaatgcata tctttatatc atctctgttc gatttggaaa acgtcttacc gtcaaacaag
     4921 aaattgatga ggcgttattg gatgaaaaag tacctcgctt ggttatgcag ccgatcgtcg
     4981 aaaatgcaat cgaacacgga atagcccctc atcgaaatgg cgatctaatt ttaagaattt
     5041 atcgacagga agaaaaaatc gttatggaag ttgaaaacag cggagaattg accgatgctg
     5101 acaaagaagc tgttgatcgc cttttaaatt gggatggaaa tgatacgaca gacattaagc
     5161 caggtcgtct cggaattcga aatgttaatt tacgcttgaa atttctttat ggaaaggaat
     5221 gtggattgac gattacctct ctcccaaatc agagaacact tgctcgaatc atcttattga
     5281 atcaatcgaa ttcttccgta agcctttata atcccgataa tcagacaaaa ttatacaaat
     5341 aaaaacaagt tttactatgt aacaaacatc ttggtcaatc tacaataaat atacaaatat
     5401 tcaaaccgtt ttcataacgg taaaagaacg ggagaaagaa atgaagaaaa gatctatatt
     5461 tatcgttgga ttggtgatta tttttagttt aatgattgcc agtgtcgctt ttgccgaaga
     5521 gattactgta gttacctctt atggcggtga tgacggaaac cgcggaaact atgaggcggc
     5581 tgtcaagatg tatgaagaac aaacggggaa taaagtcaat gatgaatcag gcacttccaa
     5641 cgaggagtgg aaggcaaaaa tcatgactga ttttgaaaca ggtgcggaac ccgatgttct
     5701 gtttttcttc aacggtgtgg attccaatcc gtttgtgaaa gccggaaaag tcgtttcaat
     5761 cgatgaaatt cgtgaggttt atccggatta tgcttcgaat atgaaggaca gcatgttggg
     5821 cgcttctccg gtggacggga aaaattattc ggtaccggta aacggatatt gggaagggct
     5881 gtttgtcaac aaaaaagtat tagacgaagc cggtgtcgcg gtaccgggag cggattatac
     5941 ctgggaacaa ttcctgaatg attgtgaaaa gattaagact gccggattta cctgtgtcgc
     6001 ggtttctctt caggaagttc ctcattattg gtttgagtat accgtattca atcatggttc
     6061 ggttgcaaat catttagata ttccgaaaac tgccgatgat gccgtcgctc aaaaatgggc
     6121 tgccggattg gaagatatca aagttctcta tgaaaaggga tatttaccgg caaatacctt
     6181 aaccgcaact gatgcagaga cttttcagtt gatggctgat cataaagcgg catttgccat
     6241 cgatggcagt tggaaaatag gctggttcca atctaatgca gacgatatta ataattttac
     6301 tgtgacatat gtccccgctc agggtgatcg tgccgcaacc gatatcatcg gcgggctttc
     6361 gatgggttat tacattactc ggaaagcttg gaatgatccc gaaaaacaaa aggcttgcgt
     6421 ggatttcgtc ggtatgatga cctctgatga tgtcgtttcc actttcggag caaccgctgt
     6481 tacggcatta aagaaaggaa caactcctcc tcaagatgca gatgctttgg taacagcagc
     6541 gcttgcgatg acaaaaggtg caaccggaat ttcgcctgcc gttcaggatc ttttgaccgg
     6601 tgaggctcgg gcggatcttt ttgcaaatgt taagaatgtt gtcaccggca aaataaccgc
     6661 tacggatgcg attgcttcaa tgatctcatt caaccaataa aaaatatcaa aatgtgataa
     6721 aaaatgaggg acagacaggg atgcctgtcc ctcattgata ataaaaccga aatcattcaa
     6781 aaaataataa agaaaaaaca taatgagttc aacgatctac aaaaataaac ttcctttaat
     6841 tattttctta gtaccggcac tgattctgat gaccatcttt ttatattatc cgttcatcat
     6901 gaatatattc aacagctttt cggatatttc aggtttagga actgccgcta agggattgaa
     6961 tgatccttgg tttaagaact atattcgatt atttcaggat cccaatatga ttaccgccgt
     7021 aaaaaataca atgatcctga tgctttcgac aatcattttt caggtaggaa tcgccctcct
     7081 tcttgcgatt tttgtggatt ccatcaaagt aggatctcag ttttttcgaa cagtttattt
     7141 cttccctttg gtgatcagtg ccacggcatt gggattattg tttaatcttg ttttccttta
     7201 tgacggcgga atgttaaatc aaatactcca aaaattcttt gggtttactc aaaatatcga
     7261 ttggaaagat gaaaatcact ttatgctgac gatgctgtct ccggttatgt ggcaatatgt
     7321 tggattttat tttgtcatca tcgtcaccgg gctcagtaat attccgcagg aaatatttga
     7381 agccgcttca ttggacgggg ccggattttg gaaacggaca tattatattt ccttaccgat
     7441 gatccgaaat gtcttgatta cttgtttgat tttggcaatt accggcgctt tgaaagtttt
     7501 tgatttaccg tgggtgatga tgggttcggg aattccgatg gatgaatcct ggttgacagg
     7561 gacttatatg tataaccaaa cattcttgcg ttcggatgta gattatggtt caactattgc
     7621 tgtcgtaatc gtcattctgg gtgtagttat ttctcaaatc gcaaataaga tttttaagga
     7681 aaaagaatat tagtatggga aacaagcgat tttcttcgaa aagaattcat tcatacagtt
     7741 ttacgacttt attttcctat accgttctga ccatttgggc aatcacaacg atttatcctt
     7801 ttgtttgggt actgatcaat tcttttcgag tcaaagggca aattcgttcg gattcttttt
     7861 ccattccgtg gccgtggtta gattctttta ctttggataa ttattccacc gctttcgaac
     7921 gctcaaatgt tttccgagcg tacggaaaca gtctcatcat ttcgattgtc gtaacacttt
     7981 tcgtaagtct ttttgcggga tttgccgcat acggattggt tcgatattct tttaaaggca
     8041 aagaaattct tcattcattc attatagcga gtatgatgtt tccggtattt tctaccatta
     8101 tcccggttta tcgaatggaa gtccattggg ggattgccag caccggcaaa ctgcctttat
     8161 ctttattgag cacgattttg ccgcagatcg cgggaaatct ttcttttgcc atagtcgtat
     8221 tgatgggatt cattcaatcc attccgattg atttggaaga atccgcattt atcgatggtt
     8281 gtaatgtttt tcagatttat tttcgaatca ttcttccggt tgcaaaaccc tctttcgcga
     8341 ccgtggcaat ttttactttt ctttggtcct ataacgacct ttttactcag atgttctttt
     8401 tacgcagacc caaagaacgg acaattactc tccttttaaa cgaaatcaat tctcaagccg
     8461 gagtaaatta cggattgatg gccgcagcag tagtgattgt tgtcgttccc gttttgattg
     8521 tatatgtatt gttgcaaaag aatattatca aagggatggt tgcgggtgcc attaaaggtt
     8581 gattttcgtg gtttgtaata agatctcgta taatttttat tatgtataaa gttgtcatcg
     8641 tcgatgatga ggaaattatt gttcgcggac tgcaaagtgt tgtaaattgg aattcttaca
     8701 aatgcgaagt cgttgcaaca gcttttgacg gtatttccgg agctattgcg gttcgaacct
     8761 atctgccgga tattttgatt acggatatca aaatgccgaa cagagacggc ttgacaatgc
     8821 tggcgggact tagaagtgaa tttccagcca tgcaggtaac aattctgacc ggttatgccg
     8881 attttgattt tgctcaaaaa gcgatcaagc tcggtgtcag ccgactttta ttaaaaccgt
     8941 cgcgtatgag tgaaattaac gaagctttgc aatttatgac cgatacactc gcaaaacgat
     9001 ctcctgccgc atcggacgac aaaatcgatg atttaagcac cgcttccgca aacagtttca
     9061 tcgtcagaca agctttagcc tatatcgaag agaatttttc tcggaaattg acacttcagg
     9121 atgtcgcgga ttattgttat gtctcccaat ggcatttatc aaaattgtta aacaaagtga
     9181 ccggtcaaag tttctatgat ttattgaata aagcccgaat tgacgcggca aagcgattat
     9241 tggaagatcc taaattgcgt ataagtgata tttctgaaat ggtaggatat accgataacg
     9301 ggcatttctc acgactattt aaaaaaatgg tggggatcag tgcaaacgat tatcgaaatc
     9361 aagttaaata agaaatcgat tcaactgatt cagaattctc aattattgag attgcaattc
     9421 cgattccaat cctaaaaaat ctgaaaagac agaaatcaat tggtcagcgg atttttgatc
     9481 ggacatttcc gcgcataagc gaatcaaggg ttccgttccc gaaaaacgaa caataaccca
     9541 gcctgaattt ttgaaatata ccttacaacc gtctcggtaa gaaacatgat cgatttcgta
     9601 cggaaaatcc ggtaatttat tttcgacgta caaaatattt tgcaaccttt ctttattctg
     9661 cggagtcaat ttaaaatcgg catccgaatt aaatacttca ccaaactgat cggtgatttc
     9721 tttgtacaat tcggataaat gctttccggt aaccgcgacc atttctgtta aaatcgtggc
     9781 ggcatagata ccgtctttcc cgcggatatg tcctttgacg gttaaacccc cggatgattc
     9841 accgccgata atcgcaccgg tttcctccat ttttgcggaa acatatttga acccgaccgg
     9901 aacttcataa caggcttgac cgaatccagc cgcaacttta tccaataagt gggttgtaga
     9961 attatttcga acggcaggac ctcgccaacc gcgatatttc aacaaataat agtaaagcaa
    10021 gaccaaaagt ttattcggat gaacaaattg tcctttatca tcgattactc ccagacgatc
    10081 ggcatcaccg tctgttgcaa tgcctaaatc acaatgatga tccaaaacga agttaaccaa
    10141 tgtgccgagt gtatgaatat tcggggcggg aaggcgaccg ccaaacagcg tatcatgtcg
    10201 atcatgaatc acttccactt cgcaacgagc ggttaataat atggtctgta aactggtttg
    10261 gcttacgcca tacatggggt ccaatgcgat tctcaatctg gcttttttta tcgcttcggt
    10321 atctaccaag gaaaggatac tatcgatata gctattcagc ggatttattt ctttgatgat
    10381 tccggatcgg attccctcat cgtaatcaag aaaaggaatt tttacttctg catttttctg
    10441 cgtcaagaat tcaatttcat ttgtaacatc ttcatttgca tcgcacccac cggcggtaaa
    10501 aaatttcaca ccgttataaa tagcgggatt atggcttgcc gtaatggtca tcccgtaggg
    10561 taaattcatt tgtttgaccg taaacatgat caaaggcgta ggggatgaac gatcgatcac
    10621 ataacaagga atttgtcgtg ctgcgaagac ttcggcagcc caatgggccg cttcttttga
    10681 taaaaatcgg cgatcatatc cgatacataa gcctaaattg gccgtaccgg ttttttccat
    10741 aaggtcaccg atcgaatatg aaagtagccg gatgttactt tttgtaaatt catctccaat
    10801 aatggctcgc catccgccgg ttccgaaatg gatcatattg tctgtttccc cataacaact
    10861 ctaacatttg tacttgtacc gcatggttta agcggtatct cggctaccgg gagaccatcg
    10921 caatacagcg ctttgacccc tttttgaaca ttgtcgggat tttccaccgt aatatcatat
    10981 tccgctcccc gccatatcct tttgacccgg aatccatccc atgattccgg aatacaggga
    11041 tcaatcgaaa gtgtggtgaa tccgggtttg atccccaata tatattgtgt tgccgcataa
    11101 taagcccatc cggctgtccc cgtcatgaac ggatgcctgg ctcttccgaa tgccgtatgc
    11161 gctttcccga caataaattg gcaataggaa tagggctctg cctgcctgat ttcgatttga
    11221 tcattttgtt tatatggaga aagggcattg taatagcgca ttgctctgtc cccgtgaccc
    11281 aaggtacatt ccgcagccca tgcccatggg ttgggatgcg aaaaaataga accgttttct
    11341 ttcagtcccg gataaactct ggtaacgaat ccgataccgt catcgggttt tgtgtaaggg
    11401 ggggcattca gcattaaccc gtagggagtg aataaatatt cctctaccgc attcatgcat
    11461 tgttctcccc tttcttggga ggcgacaccc gacaaaaccg cccaagcatt actttccaaa
    11521 tgaactttgc cctcgacatc ttgatgacag ccgattttcc gtccgtctgc ggttattcct
    11581 cggatatacc aaccgtcatc ccacagcact tcttcgcaca cttgcctgac atgctcggca
    11641 acatgccgat aatgttcggc atccttttct tcgcctaaaa agtcggccaa ttccgaaaat
    11701 tgagtgagtg cccaataatg aagaaaactg accaacgcac tctctccgcc gccgagattc
    11761 aggcaatcat tccaatcggc acgtaatcct ttgcaaatcc catgtttccc aacctgtttt
    11821 tcggaaaaat caagaattcg cttcatatgg tcataaactg atccggatcc tttgtccgca
    11881 taagttattt cttcccatac aaaatcaaaa tccccggttt ctttcagata attatgaatc
    11941 gctccgatca accataatgc gtcatccgcg caagcttgat ccaccgaatg aatcatgctt
    12001 gatttttccg gaacgggaat aatagtcggc gatttaaacg gtttcttcgc ttgttgcggt
    12061 tcaaaccaag cggggtcaaa taagtgcaac ccatacccct ccgatgtcaa agcccgtaaa
    12121 agttcgacga tccgactctt gcatttgacc ggatcggaat ggggaatact cattgcgtct
    12181 tgtgcgctgt cccgataacc caaacctgta cgtccgccca cttcaataaa agatgaaaat
    12241 cgagaaaatg taacattgat ttcgctttga taaagggtcc aaatattaat taacgaattc
    12301 attccatcat cgggagtttg aatttgcagt tttgaacatt tttcatccca aaaagttctt
    12361 aagagatcaa attggcgatc cacttcatga ggatccgcgt atttctttcg aattttcctg
    12421 ccctcttcca aagaaccttc tcccagcaaa aagatcagcc ggattttttc gcccgggtcg
    12481 attgaaagct gtttcgacag agttccacag tgattgcctc ctttttcaaa gcttgaggaa
    12541 cagtgacccg attccaccgc aatcggattt ttttcggtgc ggtacggtcc gataaagcgg
    12601 tcccgaacac aatcgaaacc atccggttca aaatcggcag taaaaaactg ataaccatct
    12661 tcttcgtaaa agagatcata ttcgatgatc ccgtcttgat atcttgatcc ggcggcgtac
    12721 aggctcattt gaaaattttg gttatccatc gcgatatgat gaaatgagaa ttcaagatag
    12781 gaaaagacac tgagatgacg ccgacgatcg gattgatttt gtatatttac atcccaaatt
    12841 tccaaaggat catcgattgc gacaaataat ttttgcgaag ctgagatttc ccgatactcg
    12901 caagaataaa cggagtaaga aagcccatgt cggcattgat attttgcttt atcaagcggc
    12961 aatccgacag gctgccatga aattgtccaa aattctcccg tatcatcatc tcgaatatag
    13021 acatagtgac cggggaaatc cattgggaca ccgtttggtc ggaatcgggt aattctgtga
    13081 tactccggtg aaccgcagaa cagatatccc cccgctgtat gattgaacac accacacata
    13141 ttttttgtcc cgatataatt ggtccacgaa acgggaacat caacctgttc caccacatat
    13201 tcccttttgg catcatcaaa aaacccaaag cgcataatga atcctcggaa tcggaaaatt
    13261 tgaattatga aaatatattc cgattataaa attttcagaa agaaaaagaa atggataata
    13321 ctgtcaatta ttgaatccgg ttgttatagt acaattgcgt gggtgtcctt tacagaggag
    13381 ccgttttacg ctaaaattga ctcgctgtcg ggaaccgacc gattattttt tttgatgtgg
    13441 ttgaagaaac agaaaaatgt ttgaaaatct gaccgaacga ttaaatcata tatttgcggg
    13501 attgacccgg cgcggtaaat tgagcgccga agacgtcgac gccgctatgc gtgaagttcg
    13561 gattgcattg ctcgaagcgg atgttcatta ttctgtcgtg aaaacatttg tcgcaaacgt
    13621 cagggaacga gcgatcggaa cggaagtatc cgaagcttta aatcccggac aaatggtcat
    13681 taagattgtc aatgaagaat taatcaaaac actcggtccc gccgaaaagt taaacctcag
    13741 cggagaaaaa ccgcacgtta tcatgctggt gggtttacag ggatccggaa agacaaccca
    13801 aaccgccaaa cttgcaaaaa aattacgcga acagggtgaa cgcgttttaa tggtcgctgc
    13861 ggatccgtat cgaccggcgg caattcagca attacagaca ctcggggaac gaatcaatgt
    13921 tgaagttttt accaaagaag gcataaaacc tcccgaatta tcacgccaag cgttggaata
    13981 tgcaaaaaaa ggcggcttcg gtatcgtttt aattgataca gccggtcgat ctcaacttga
    14041 taatgttttg atggaagagc tacaggctat ccaaaaaata acgcatccga atgaaattct
    14101 tttagttgtc gactcgatga tcggtcagga agcgcttcat gttgcggaag gctttcgcga
    14161 tgccgttcaa atcagcggtt tgattttgtc aaaaatggat ggtgactccc gcgggggggc
    14221 ggctatttct gttcgaagtg tgacaggtgt ccctatcaaa tatttgggaa ccggtgaggg
    14281 tttagatgcg atagaggtgt atgatccttc tcggttgtcc tcacgtattt tgggaatggg
    14341 tgatatcatt gggcttattg aaaaagcgga gtctgtttat aatgaaaaac aggcaaagca
    14401 acaagcgaat cggatgcttt caggcaaatt taccttggaa gattttgcca cccaattgcg
    14461 acaggtaaag aaaatgggtc cggtgcagca gattttggga atgttgccgg gaagcttagg
    14521 gcaggcggca aaacaaattg atccgaaaga agctgagaaa agtctcaaag tcacagaagc
    14581 gattctcagt tcgatgacat tgaaagaaag agaaaatcct gatattctga atgcaagtcg
    14641 gaaacgacgt attgccagcg gatcagggac cgatgtacag gaagtaaacc ggcttttacg
    14701 acaatttcgt gaaagtcaaa aaatgatgaa aacgatgcaa aaaacaggtg gaaggggtct
    14761 ttccaatctg tttcgctgat tggttttgac tctgatgatt tttagaccct gcgcactcct
    14821 tcagcgcaac cctcatctca gagaagcaac agcgaataat taagtcaaaa aagaaggaac
    14881 aggagaaaaa atttaaaatg gttcgtattc gattacgccg aaatggtttg aaaaatcaat
    14941 ccagctaccg aattgtagcc gcagataaag agagcccgcg gaatggtcgg tttcttgaaa
    15001 ttctcgggtc ttataatcct cgcacagaac cgttcacttt tcaggtgaag gaagacagaa
    15061 tctactactg gatgagcaac ggagctcagc cgagtgaatc tgtcgtaaaa ttatttaaaa
    15121 gcgtaggatt aatggaccga tttgaacgct acaaaaaggg tgagtcaatc gatgcattga
    15181 tggaagaagc aaataattat tatgctcaac gcaaagtcaa tgtaaaaaca cgtgaagctg
    15241 cgctgaagtc ataatgcagc acaaagaaga tgtcaaaatg cagagaaatt cttccaataa
    15301 gggtgatttt gccaaggatt tggtagaata catcgcactt tcactggttg aagataccaa
    15361 cagtgttcgc gtcacacagg agcgaagcgg taaccgagtg cggttagagc tgcatgttgc
    15421 caaggaagac atgggtagga ttatcggcaa gggcggacgg gttgccaatt cgatccgttt
    15481 attgcttcga gtcgcttccg caagagatgg caggcaaatt tcattggatg taatagaacc
    15541 cgattcttaa gtcgttggat ttcacagtga aggcgaaaag tcatcgggat caacaggaat
    15601 tcaatcaaca aggctcgtca aatattgacg agcctgaatt tatttgtatc ggaaaaattc
    15661 atcggtcgca tggcatcaac ggtgccgtta taatggatcc gatgacggat tttcccgaac
    15721 ggattcgttc cgggaggaag gtttttgtcg gaacgagtcg ggctcccatg gtcatcaaat
    15781 ctgctcgttc aatgcctccc ttctttttgg ttcaatttgt tgaaatcgac agtcccgaaa
    15841 aagcatcgga gttacgcaat caatttgtct ttgtgaaaag aatcgatttg ccgaaactcc
    15901 ccaaaggcag atattatttt catcaattaa tcggtctgga agtgtttgag ataaacggta
    15961 cgcggatcgg taaagtgaag gaaattttgg agaccggtgc aaacgatgta tatgtcatcg
    16021 aaaaagagga tcaaacagaa gtgctggctg ccggcattcc ggaagtgatt cgggaaatca
    16081 atcttgaaga aaagaaaatg gtagttaatc tgccggaatg gatataaatc aaaatgcccg
    16141 accaattggc ttattgggtg gcattcaatc atattaaagg ttttggttcg gttcgttttc
    16201 aacttttatt aaattacttt tcatcactcg aagcggcctg gaaggcaacg ccaattgagt
    16261 tggctgccgc aggagtccat gaacgacaaa tcaaagcaat tcttgatttt cgtgaaaaaa
    16321 attctccgga agacctgttg aacaaaattc aaagatcggg gatatcggtt gtaacccgaa
    16381 tggatgacaa ttatcccgct ctcttaaaac tcattgcgac gcctccgcct gttctctatt
    16441 ttaaagggac actgccggaa caggataaaa aatgcattgc aatcgtcggt acccgaagaa
    16501 tgactgctta cggaagcgaa atggcacgag agatcagcgc ttttttggca tctcgtggag
    16561 tcgtcatcgt ttctggattg gcgcgcggga ttgacagtgt cgctcatcag gctgctttga
    16621 actgcggcgg tgaaacagtg gctgttctcg gctccggtgt ggatgtaatt tatcctccgg
    16681 aacacaggct cttagccaag aacataacgg aatccggtgc tttaatcagc gattatccac
    16741 ccggaacggc tccggaacgg acgaattttc ctccgcggaa ccggatcatt tccggaatgt
    16801 gcagcgcatg tattattatc gaagcggggg agaaatccgg ttcgctgatt acggcacgtt
    16861 ttgccgccga acaggggcgc gaagtattcg tgttaccggg gaacttaaat gcaccgcagt
    16921 cgatcggaac aaaccgcctg atccgggacg gtgcccgacc tttacttcag aaagaggaat
    16981 tgttggagtt cattgaatct tatgaatccg tcaatccggt cgctctaaat aaaaaaatcc
    17041 agatatcgtt tgaggatccg ttggaacaga aaatcttgga tttaatcgat gtcgaagcgc
    17101 tgcatgtcga tgaaatttca agacgattgg gacttaccgc aggaaaggct tcatcgatat
    17161 taaccatgtt ggaattgaag ggatttgtgc aggagacagc cccgcagact taccgaaaat
    17221 atttatccct tttttaatat ttcatattga acaaaaatga atgagaattt cttcattgga
    17281 gtgaaattct gattaagcct tgataaaaga cggattcaat ggcattattc ataagaattt
    17341 caaaccggac aggaagacag gcaatggagg aaaaatggaa aatgactttt atgccgtgat
    17401 tatggcaggt ggcggaggaa cgcgtctctg gccgctttcc acaaaagatc atcccaaaca
    17461 aatgttaaaa tttgcgaaag atgagaccat gttccaaatg actgtctcca gattgacggg
    17521 aaaaatacct tttgagaata tattggtcgt aaccacagaa gaccaggctg cgaaactgaa
    17581 acaacaatgt aatgacatac cggaagaaaa ttatctgatc gaacctttgc ctcggggaac
    17641 cgccagtgtg atcggactgg cggcgattca cttggaaaag agaatgcccg gttcagtcat
    17701 ggctgtgata ccgtccgatc attttgttct gaatgaaaaa caatttttag ctttactgga
    17761 gaatggatac cgggctgcaa aagatcaaca cttagtgacg ttgggggtga aaccgtcctt
    17821 tccttctacc ggaatgggat atatcgaaca aggagaaaaa ataccggttg gttatgattt
    17881 gtaccatgtc ggacaatttc gagaaaaacc ggataaagaa accgcggaat attttgttca
    17941 atcaggaaaa ttctggtgga atgcgggaat attcgtttgg tcaagccgcc ggattttgaa
    18001 tgaaatagcg atgtttctcc cggatctctc aaaaaagttg gatataatca acgcgactat
    18061 cgatcggccg gattataccg ataccatgga gaaaacctgg cgattaattg agccggcaac
    18121 catcgattat ggtgttttgg aaaagacaac cgatgtcgtt tttcttccga cttacgatct
    18181 cgggtggaat gatatcggat catgggaatc tttatttgat gtgatacctc ccgatgaaaa
    18241 cgggaatatt atcataaact gtaaatttca ttcgattgat tccgacggag ttttggcgtg
    18301 ttccgaaaat aaagagaaaa tgattgtatc cattggggta aagaatatga tcatcatcga
    18361 gacggaaaaa gccgtattgg tttgtccgcg atccgaaacg caaagagtac gtgagattgt
    18421 cgatttttta aagaaaaaca atttaaccgc atatttatag gagaaaagtt tggaagctta
    18481 ttgcctgaaa tgtaaacaaa aaagagaaat tcaaaatccg atcgcggaat ataccgcaaa
    18541 cgggaggcct atcacgaagg gaacctgccc tgtttgcaat tcaaaactca gtctaatcgg
    18601 gaaaacagcg gctcatgagg ggttggaaaa gcctcaaata acgaaagctg ccaaaaaagc
    18661 aaattcaaaa aaagttgttg ctaaaaccca ttcgaaagat ccaaacccta aaaaattaaa
    18721 cggttcgaaa aacggaacaa atacaacccg aggaatcggg cagaagctgg ttatcgtgga
    18781 atcgccggcg aaagcgcgaa ccgtcggaaa ctttctggga aaaggatata ttgtaaaagc
    18841 ttcgatcggt catgtccgtg atctgaaaaa atcaacttta tccgttgatg tggaaaatgg
    18901 ctttacaccg gaatatcggg ttcccaatga aaagcgagct ttggttaagg aattatcaac
    18961 gctggcaaaa aacagcgatg aagtttatct ggcaacagat cctgatcgag aaggagaagc
    19021 gatcgcctgg cacttattgg aggctgcgga tattgatccg aaaaaaacgg aaagagtcgt
    19081 ctttcatgaa attacaaaac cggcgattgc ggaagctttt aagaatccac gcgatattaa
    19141 tatggatctg gttgatgccc aacaggctcg tcgaattttg gacagattgg tcggttacaa
    19201 tttaagtccg ttactttgga ataaggttca acgccgttta tccgccgggc gtgttcaatc
    19261 ggttgcggta aagttaattg tcgatcggga acgggaaatt gaaaattttg ttcctcgtga
    19321 atattggacg ataaccgttg aacttcaacc ccaagatatt aaaggcactt tcctttccag
    19381 attagttaag atcgatcaag ccgatccgga attaaataac gaagagcagg ttcagtttca
    19441 tcttaatcat cttcaatccg ccgattatgt gatcgatgcg attaagaaga atgttcggca
    19501 ccggaaacca tccgcgccgt ttacaacttc aactctgcaa caggaagcgt ctaaaagatt
    19561 gggattaact gccaatcgga ccatggctct ggcgcaacag ttatacgagg gcatcgatct
    19621 cgggagtgaa ggatccgccg gtttgataac ctatatgaga accgattcaa ccaacatttc
    19681 cgaaatcgcc caaaatgaag cacggaaata cattcagcag acctatggtt caggctttgt
    19741 tcccaaaaca gcgccggttt ataaaacaaa aacacaacgg gcacaggaag ctcatgaggc
    19801 aattcgtccg accactgtaa accgaacccc ggaacaaatg aagccttatc tcaatcggga
    19861 tctttttcgg ctgtatcagc tgatttggca gcgttttgta tcctctcaga tggaggatgc
    19921 tttgtatgat acactgacga ttgatgtcag cgccaaggat gcccatgatt atcttttccg
    19981 ttcctcggga tcaaagctga aattccaagg atttctggct gtttatgagg aaagcaaaga
    20041 tgaggatttg gtaatagatg aaatggaaaa caccaaattt cccgaagggt tagctgtcgg
    20101 gcagaaactg caatgtattc atacaattcc cgaacaacat tttacgcaac ctccggcacg
    20161 atataccgaa gcgactttgg tgaagacact tgaagagaac ggtatcggtc gaccgtccac
    20221 ttatgcctcg atcctttcga cgattcaaac gcgaggttat gtcgttcgcg aggcgaagcg
    20281 tctttatccg accgaaaccg ggattttggt caacgatctg ctcgtaaaca gttttccgaa
    20341 tgttgttaac atcggtttta cctctcagat ggaaggtgaa ttggatgatg tagccggtgg
    20401 ggagaaaaaa tggcaaacgg tggtgggaga tttttataag gaatttgagc cggcttnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaga aaaaatggca aacggtggtg
    20581 ggagattttt ataaggaatt tgagccggct ttgaatcaag cgcggaaaga attgccaaaa
    20641 gcacagttgg aaccggagaa agtcggtcgg aaatgcccgc aatgcggaaa agacttggtt
    20701 attcgaaacg gcaaatacgg aaaatttatc tcttgcagcg ggtttccgca atgccgttat
    20761 acggaacaga tagtggagaa attggatgtt ccgtgtccga aatgcggaaa agagctgatt
    20821 gttcgtcgct ctaaaaacgg aaggattttc tatggatgca gcggctatcc ggaatgtcaa
    20881 tttacctctt ggaaaaagcc ggtaaaacag aaatgtccga attgcggcgg gatgttggtg
    20941 gagatgaatc gggatacgct acaatgcata gaatgcaaga cgaaagtcga aaataaagat
    21001 tccgaataaa aacgatcgaa tttggaaata taattgggat aacgaatcgt tgcagagctt
    21061 tgtaaataaa aacgcttaag aacccattca gttctatgtg gaggtcccga tgaaagaaaa
    21121 agccaaggca tttgttgagc aaaatccatt ttggacggga atcattgccg gatttctgat
    21181 ttgttttgct ctgatcggac tgctcagcgg aatcggagct gagcaaacaa acagctttaa
    21241 gaatgccgac caaattctca aagaagatta tcttcggatg acggttgaag attatgcctt
    21301 cacaaaaaac gccgatcggg ctaagtggag atacaaacaa ctcggagagg aagctccctc
    21361 tctgattgca ttactcaaaa aagatgattt gacagatccg ttggctttat ttgaattttc
    21421 ccgagtcgtc ggggatatga cactttttta tgaagggtcc gaaattgttc cggaacagac
    21481 aaatcaaccg gttagccaag aagagtccgg cggcgtctcc ttaatcggaa aaattttcat
    21541 cttattgatc ttattgattt tggttggagc gattgtattg tttttggttt caaataaaag
    21601 cagttcggtc aaagaaaaaa cgtctcggtt tttcgctcgg tttcaaaata aaaaagaacc
    21661 gtcttggaag gttaaaacgg atgatcaatt tgatctcgga aaagaaatta aaggacatcc
    21721 gatcggatca agagaaacga aagaagacga agacctatat aaaaaagccg atcgaatctt
    21781 gacaaatgaa ttcgaattat ccgatgcctg ggttgaagat gaaactgggg aagaaaatga
    21841 tgaaatcaat tcttcggata cttcggagac cttaatccaa acgaaacccc ggtccgatgc
    21901 gcctgaggaa agtgatgttt cggatgaaga cggattcaaa aacgtgaata taactcaaaa
    21961 agaaggcgaa gaatcgccgg aaaccgaaga aaatcttggg aattcggaag atcatttatc
    22021 gggaagcgaa ttctttgatg aagacgggtt tgattccgaa cttgaatctt ttgaggattc
    22081 gcagccggaa gacgcaattt cggaaaaacc tgatgaagaa gtgcaaattc cggataccga
    22141 accggatatt catccggaag aaaaaaacat cggttcggat ttttcgttta agcccgaaag
    22201 cggtagaaaa aacgaaactt ccgagacgat ttcgaaggaa gatctttcat ccgaggatga
    22261 accgctgatt cactatcaga cgatttacaa actgggcgat gattttttcg atgaaacttt
    22321 ttctctcgat gaaggagata aattcatcgg agaatgcgga atcggaattg ccgaaacgat
    22381 caacaatacg gacccgaaag cggttacggc ttttgagatc tggctgtttg atcgggaaga
    22441 ttctcagact ccgactcatt ttttgttgag tgattatgcc tataacaacg atgaattgtt
    22501 ggaacgattg aaaaataagg gcagattcga tctgatcgaa cgaaatcgag attatgaggt
    22561 gaagactcaa aatctgagga tgatcgtaac gattaaggat atggaatacg gaacggaaag
    22621 cagcgataag aaaagctact ttaataaagt gacatttgac gt
//
LOCUS       DAOSMM010000034        22033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2364_length_22033_cov_18.988807, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000034 DAOSMM010000000
VERSION     DAOSMM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 22033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22033
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2364_length_22033_cov_18.988807"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1726
                     /locus_tag="PK898_06255"
     CDS             158..1726
                     /locus_tag="PK898_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU19672.1"
                     /translation="METILSLYERTLSEKNPDTTAIRWVDSHLNMTPITWRMLENESN
                     NRAIYLTQLGCRPGHHVFIFLESSIEIWYFFWAALKCGAVPCVLFPDFGSDALGARLQ
                     SGRADWIITDRWNEKNVGAIRSCFSLKKIILLEEPSGIPDTRMALFHRESTTNDSFPL
                     YPAIETDPAFIVFTSGSTGFPKAVVHTHRCAESILRSMQNVLQVQNDDIYWCTAHPAW
                     ITGTVYGVIGPLIAGVESVQYSGNFHARRWMPILQNQEVSVWYSAPTAFRSLMTQPIE
                     FFTKFDFSNLREIFSIGEPLNPAVYYWGKEIFQREIRDTWFQTEAGTIRIANLPGKLI
                     KPGYMGMPVDDALPVILDEQGKECSAGQVGRLCLKAGWQSCFTDYFGQHDAYLSKFQN
                     GYYQTGDLAFQDMDGYFCFVGRDDDVINTSGHLVGPFEVESVLLEQPEIAEAGVVAAP
                     DPLLYEKVAAFIVLKTGVEWTSELETRLAVAVTTRLSTQATPRQFIVVNALPKNPAGK
                     ILRNELRKRLRSQS"
     gene            complement(1790..2995)
                     /locus_tag="PK898_06260"
     CDS             complement(1790..2995)
                     /locus_tag="PK898_06260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU19673.1"
                     /translation="MKKLSAGNLFWNQTIEPFACFNPLQNDLETDVLIIGGGMSGNLC
                     AHEIAGRNHSVVVCEKDQLAMGSSSASSGILQYLNDEMLYRLIESIGEKKAVLFYQMC
                     LDSMKDLINLSRKMEQYGGLMPRKGVFFASAKEDVPELDKEYKALSDHYFPVSYLDHA
                     ELMRIYGIDRPAALETSMNAEVNPYQFLQTLMKLNVSLGVQYYEKTGIKKVKTDKDQI
                     IATTKNGNRIQAKYVVFATGCSVIYPELKKKMKVVNTYTFASSVSKRPLWQDHVLFRE
                     TRPPYLFFRMTEDNRVIAGETDKKADSPEKKKGAIDKRNREILERVQSLMPNLDVKVE
                     YSWNTLSADSKDHIPFIGPSKKDPRRIYLIGVGGNGLVHSMAGASIIADYIDGIKNPY
                     AGVVKAKRK"
     gene            complement(3112..5364)
                     /locus_tag="PK898_06265"
     CDS             complement(3112..5364)
                     /locus_tag="PK898_06265"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU19674.1"
                     /translation="MKPEVKRYTAEVGGKTITFETGKLAAQAGGAVTIQLGESVVFSA
                     ATMGDEVHEEQDFFPLTVEYEERMYAGGRIPGSFFRREGRPAGDTILIGRLVDRPIRP
                     LFSQDIRNEVQVILYSLSADFENQLDILAINAASASLAISDIPWDGPVGAVRVGLING
                     EYVINPTFQQMEDSDLDLRIAGTADAILMVEAGSNEVSNEAMVGAIEAGHRAIQPLIE
                     AQLKMAAEVGKPKREVKRSCIPQTTVDTVFEKYNQSMETLLDAPHDKHELADRLAELK
                     ADAVEFFAGEDSEKQSEYGNAFEEAYRKVIRSRILDRHLRPDGRALNEIRPIWCEVDV
                     YPRAHGTGLFTRGETQVLTSATLGTPRDAQEIDSLSPVENKRYIHHYNFPPFCTGEVK
                     RLGGQSRREIGHGALAERALVPVIPSEEDFPYTIRLVSEVMSSNGSSSMASVCGSTLA
                     LMDAGVPIKAPVTGIAMGLIKEGDDYVILSDIQGAEDHLGDMDFKVAGTEKGITALQM
                     DIKIRGITTEIMRKALDQANEGRHFILGKILEVIPAPRKDLKPHVPRLITVQVPIDKI
                     GAIIGPGGKNIRTLQEATNTKVDIDENGLVYISATNVGDSDAARDQILSMIETAIVGN
                     IYTGKVVRVTDFGAFVEILPNTDGMVHISQLDSERVNKVEDIVNVGDEITVMVTGIDE
                     NGKIRLSRQAVLEGWTLDQAVAADSKKPSSGHRPGNSDRSRHNSDHKSGNRGDDDRRH
                     SGGGSYKKQR"
     gene            complement(5579..5851)
                     /gene="rpsO"
                     /locus_tag="PK898_06270"
     CDS             complement(5579..5851)
                     /gene="rpsO"
                     /locus_tag="PK898_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU19675.1"
                     /translation="MALTKEEKAEIFKNFGLHEGDTGSVEVQIAMLTKRIADLTNHLK
                     TSKNDESSRRGLLKMVGQRRRLLAYLRKNDYASYVALNEKLGLRVK"
     gene            complement(5981..6796)
                     /locus_tag="PK898_06275"
     CDS             complement(5981..6796)
                     /locus_tag="PK898_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19676.1"
                     /translation="MPAVNLLNLRKEITSLLWEFTDPESFRDKLAQLLDHYRIMSFRS
                     GSGVLTSYQTPELHVPQLVLHELELALYQTIREQPQAALTLCDSLWNDVNLEMHTIAT
                     RMLGQIPIEYMDQVLTRINEWTQKGLKPSELKVFFRNAAGLIRQKQPEKLMRSAQDWC
                     AEPDNKNRERGIQLLIYLVEDPAFINLPAVYTALEKMLPDADAVLQPDLTELIGLLLS
                     RSEIETSFFVRQMLENPDSGKIIRRIARKLLPKIPESSRESIQNLLRTSQKEN"
     gene            complement(6830..8266)
                     /gene="proS"
                     /locus_tag="PK898_06280"
     CDS             complement(6830..8266)
                     /gene="proS"
                     /locus_tag="PK898_06280"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOU19677.1"
                     /translation="MAEDKLTSRSENYSDWYNQVVLRADLADYAPVRGCMVVKPYGWA
                     LWENIQRELDRRFKETGHVNAAFPLLIPKSFFEKEKEHVEGFAPELAVVTIGGGEELE
                     EPLIIRPTSETIIGYMYSKWIKSYRDLPVLINQWANVVRWEMRTRLFLRTLEFYWQEG
                     HTAHATAEEAQEETLRMLDVYADFAINEAAVPVIKGRKSETEKFAGAINTYSIEAMMG
                     DTKALQAGTSHYLGQNFAKAFDIKYLDQNNTLQYCWTTSWGLSTRFIGAIIMTHGDDQ
                     GLILPPRLAPYQIVIVPIYKNDQERSVVMEYIEKVQSQLKGFRYRLDDRSELTPGFKF
                     NEWELKGVPLRLEIGPKDVEKHAIMAARRDIPGKAGKISLSIENLDLKIENLLNEIQQ
                     SLLEKATEFRDQNIHFADSYDELKQIVENGWAEAWWCGSAECEAKIKEETKATTRCMP
                     LDEKIRGKGKCVYCGAEAEKRIYFSKAY"
     gene            complement(8310..8531)
                     /locus_tag="PK898_06285"
     CDS             complement(8310..8531)
                     /locus_tag="PK898_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19678.1"
                     /translation="MNSEEIDFSRSEIEIFWDGILSRERATILSTFLSVTLAEQESCL
                     KHLNRMAHEEGWHEEQRKSAQIALDVLNR"
     gene            8706..9890
                     /locus_tag="PK898_06290"
     CDS             8706..9890
                     /locus_tag="PK898_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU19679.1"
                     /translation="MTATIDDYGLVFSGGGTRGAYEIGAWKALNELGIKIQGVVGASI
                     GAINGALFLQGDIDKAEKIYRGIRVEDILEIEQPIDPNHDLLSPSNLTGLIKELIQQK
                     GLSNSGLKNLILQEIDLDKIYNSDLDYGLVTFSIDSLSPIEKFKEDIPKEELVDYIVA
                     SANFPIFKPHVAGGSRYIDGGIYDNMPINMLIKKGYRKIIVIDLSGEGFTRKMERKDG
                     VYLKIIRASEDLGGTFEFDQEKITRNIQLGYLDTLKAFQKLQGHSYYFTPEYFNHLLE
                     NFNLDIIYGLESAAKHYGIDRYQIFKDDSFLSAILEKHRQARKQFNDYQTDSIMNRVI
                     KSPSLITKMINSGMAIAWFEEMVMSWPGQKFAALQRFFGEYMMAAYAMIELQFYMDDF
                     SF"
     gene            complement(9969..12041)
                     /gene="fusA"
                     /locus_tag="PK898_06295"
     CDS             complement(9969..12041)
                     /gene="fusA"
                     /locus_tag="PK898_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU19680.1"
                     /translation="MKEYSTDSIRNIALVSHSSAGKTMMTEAFLHFTGATTRLGKIED
                     GTTVSDFDDEEKRRTISLYSTVIPIEYKDKKINVLDTPGYNDFIGEVVSALSVSDGAC
                     VLLDSVSGREVGTEIAWKYVNKFNLPRIVVVNKMNRENADFKKAMASLADYTDMRLIQ
                     VQIPWGEKLDFRGVIDILTMKAYDGAENKIVDIPAEYMEEAQQLRAALTEAAAESDEE
                     LMEKFFESMELSDEEILRGFSKAVKKGDFVPVYCAAGGQEIGVAPILDAFINLMPSPA
                     ERKPIVVKDKKGNDVEISCDDSGPLVAYVWKTTADPFVGKQTFLRVYSGMLTSDSRIW
                     NGTRSVEERYGNLNIPMGKDNIPVKTVHAGDICSVAKLSETVTGDTFSDKANPLTVPV
                     PEYPHALYRVAVYPKTQADSTKISPTLTRLCEEDMTLSWYNEMATNQTILQGMGDQHI
                     DVAIRRAESKFMVNLLTKEPRVPYQETIVKEASAMYRHKKQSGGSGQFGEVHLKVKPN
                     PDAEFEMHNDVFGGAISASYMPAIEKGIRNAMKEGILAGFPIHGIYVSVFDGKEHPVD
                     SKPIAFETAGREAFKLAFADAGPVLEEPIYTVKITVPEEYMGDVMGDLNTRRARILGM
                     ETEKGESTVTALVPLAEMQRYTTQLRSMTGGRGVFTMEFSNYEVVPPHITQEIIANRE
                     KEKKEAEK"
     gene            complement(12265..13659)
                     /locus_tag="PK898_06300"
     CDS             complement(12265..13659)
                     /locus_tag="PK898_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOU19681.1"
                     /translation="MSQNEVQSIGYLPDERPPFFKLVLYALQQVIVMFPATVTVAIIT
                     GFHISTTIFASGLATFCFILVTGRKIPLYYGSSFSYISAIAGIMAAKAAEGADLNTQI
                     QTAQFGIIMSGFVSIFAGLIIKRFGTESIEKVLPATITGPIAMSIGLTLAANALLDAC
                     AVPAGVAEESIDLVRNYAWTISLITLLSTIFFTVYLKGFAGQLTLLFGPIVGCLVTVL
                     ISKFTGFDFFRTLSEGNGTGLFALPVFTLPKPSLAAVAAIMPIAIATIPESTAHLYQL
                     DIYVNNLAKVKKSEKRYNIADKLGLNLMGDGIADIVSALVGGPAGTNYGENISAMTIT
                     RVFSVPVLIAASIIAMIISCFRPLINAIYAIPQPVIGGLEIYLFGAIAAQGIAIMVDK
                     KVDFFDAQSIALIASIMVIGVGGQYAFGGSIPFFGIQVPCIAGAAIFGIVLNLLLSFG
                     KKPALSTQNGTESK"
     gene            complement(13685..14248)
                     /gene="pyrR"
                     /locus_tag="PK898_06305"
     CDS             complement(13685..14248)
                     /gene="pyrR"
                     /locus_tag="PK898_06305"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOU19682.1"
                     /translation="MPSIKAVVMDETAMNRALMRISHEIIEHNHGTDKLGLIGIKRRG
                     VPLAKRIAEDIRIIEGVQIPVGSLDITLYRDDLSSIAEMPSVNASEINFSVTGLNIVL
                     VDDVIYTGRTVRAAIDALFALGRPASIQLAVLIDRGHRELPIRPDYVGKNIPTSRNEL
                     IAVNLPEFDNRTNVEIHDSENYSNHGQ"
     gene            14856..15974
                     /locus_tag="PK898_06310"
     CDS             14856..15974
                     /locus_tag="PK898_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOU19683.1"
                     /translation="MKKTLLTVLVLVMLVTSLVGFAPAKEDDFVIGILTGTVSQGEEE
                     FRAAEKMAAKYPGKVITATYPDNFNDEVETTIARALELVSNGADLIVFVQAVPGAAAA
                     IDKVHELKPDFPFIAGVAGEDPDVIASKADVVLGVAEIQMGTKMAEQAAAMGAKTFVH
                     YSFARHMSYATIVARHEILKKRCEELGITFVDATAPDPTGDAGVTGAQQFILEDLPRQ
                     VEKYGKETAFFNTNCGMQPAMIQAVLATGAYYPQQCCPSPYHGYPEALGISIPEDKQG
                     DVSYIIEQIKAKVAEAGMTGHFSTWPVPVNMMMVEAGVEYGFDYLNGEIDNLADVDHW
                     TDLFMDIANGSKVEVFPLELNGVVYDNYLQILSDYITF"
     gene            16215..17822
                     /locus_tag="PK898_06315"
     CDS             16215..17822
                     /locus_tag="PK898_06315"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU19684.1"
                     /translation="MDSEYVLKMENINKEYYGNKVLKNVSLNVKRGEIHALVGENGAG
                     KSTLMNILFGMPVIHSTGGFEGKVLIDGNPAVINSPLDAMQAGIGMVHQEFMLIPSFT
                     IVENIKIGREICTPNFISKILHDKNLETLDMEKMAADARAALNHVGMDIDEYTRVAGL
                     PIGYQQFIEIAREIDKTGIKLLVFDEPTAVLAESEAERLLEVMRKITSFGIAIIFITH
                     RLEEVMAVADTITVLRDGEVVGIRKNNETSVIELAKMMIGRHIESMAGKEQTGRNLSF
                     DRIALETKNFYVDMPGEEVKGVDLKVREGEIFGIGGLAGQGKVGISNGIMGMFPAAGE
                     VIIFGKNVRYSVIGETLKHHTAFVSEDRRGVGLLLDDSIALNIIFSAMQTQNRFLRKF
                     GPLSQINSFEVQSFAKEMIHKLDIRCTGPDQAVGSLSGGNQQKVCLARALTLNPQILF
                     VSEPTRGIDIGAKKLVLDELLKLNREKGVTIVMVSSELVELRSICDRIAIVSDGKVFK
                     ILNPDASDIEFGLAMSGTLAEEELANG"
     gene            17815..18867
                     /locus_tag="PK898_06320"
     CDS             17815..18867
                     /locus_tag="PK898_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19685.1"
                     /translation="MADRLKSIYHSFGLPRIIIILFFLALLIAGGLLEIPVPSLISDV
                     LVRFGMNGILVLAMVPGIQAGIGLNFGLSLGIVAGLLGGVISIESGFSGFAGLAVAIG
                     IGILLSIVIGIGYGLLLNSIKGSEMTVSTYVGFSAIALMNIFWLILPFHNPDMVWAIG
                     KGLRATIDLSSRYKHILNEFMTFNIPTSAGDIVVPTGLILFFFISCFIVWLFLRSKSG
                     IAMSAAGGNPKFALAAGIDENKTRILGTTISTVLSAVGIIVYAQSYGFYQIYNAPLMM
                     SFAAAAGVLIGGASISRAKISHVIIGTFLFQGLLVLGLPVANKLLPEGNLSEVMRIIV
                     SNGIILYALTKSQGDK"
     gene            18867..20189
                     /locus_tag="PK898_06325"
     CDS             18867..20189
                     /locus_tag="PK898_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19686.1"
                     /translation="MTKSTARKITSFLFAYKVPVLFILLSIGGILVSGQSLNFILMEL
                     ASRFGRDTFLVLALIIPVVSGMGLNFSIVVGAMAAQIAIFWVMHLLQTVIPTCPPVLA
                     FLLCVLISTPIASLFGYLAGKLFNMMKGAEMIGGLILGYFSDGLYQLLFLFIFGGLIP
                     IANKTLMIATGIGVKNTIDLSSTKKVNGLKYAIDNIWKVPLTEFLQLVFAGVVLYAIG
                     AFLYGKIKNRKVLSGRFWILFGISAILFGVSFIPAFIQFSMIISIPVVTYMLIAVFCL
                     FIQWFLKTRLGQNMRAVGQNKVVANAAGINVDKVRIISIIISTVMAAFGQLIYLQNIG
                     VFTTYGAHKNVALYSIAALLVGGASVYKATIGQAILGIILFHILFIVSPLAGQTLMND
                     AMIGEYFRVFICYGVIAMSLAMHAWTMKNTKEKKVIEPIALESEVKDN"
     gene            20418..20927
                     /locus_tag="PK898_06330"
     CDS             20418..20927
                     /locus_tag="PK898_06330"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOU19687.1"
                     /translation="METIFAPWRGEYINGIGNHAKNPDNPQECVFCSAIKATDDFERY
                     IIYRGINAFVILNLYPYNNGHMLIIPYNHVSDLSQLNTETHHEMMDLIVKAQKVLQDV
                     YHPHGINIGANIGTAAGAGIDQHLHFHILPRWTADVNFMTTIGQTRVIPEKLDETWKK
                     IRASWQKID"
     gene            21065..21313
                     /locus_tag="PK898_06335"
     CDS             21065..21313
                     /locus_tag="PK898_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19688.1"
                     /translation="MDYSMISKLEKAKRYAKERDRFKIHSLEATVEGSNGPHVVKYKD
                     GKWECDCSFFQGRGRCSHTMSLEIILEGMVDIAYEDPQ"
     gene            21436..21900
                     /gene="bfr"
                     /locus_tag="PK898_06340"
     CDS             21436..21900
                     /gene="bfr"
                     /locus_tag="PK898_06340"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOU19689.1"
                     /translation="MKGSEKVIQALNANLKDEMTAIDQYILHAEMCEDWGYSKLHDSI
                     EKRAITEMKHAEALIARILFLEGIPIISELGKMSIGKDVEEMMAFDHKAEHDAIQSYN
                     NAIKVCVEEKDNGTKELFEATLKDEEDHIDWIESQIDQISQMGIQNYLAKQV"
BASE COUNT         6019 a         4912 c         4759 g         6343 t
ORIGIN      
        1 aatcagaaaa tattccgatc gaaatggggc aaatgaaatt atccgttaca ataactgcag
       61 atcgattatc ccgcaacccg tgaatttata ttcgttattt ttgtccaatt atcggaaggg
      121 gattaaatcg ataaattaat tcaaaaagaa gcgaactatg gaaacaattc tctctcttta
      181 tgaacgaaca ctttcagaaa aaaatccgga tacgacggca attcgctggg tagacagtca
      241 tctgaatatg acaccgataa cctggcgtat gctcgaaaac gaaagcaaca atcgggcaat
      301 ctatctgaca caactgggtt gccgtcccgg acaccatgtt tttatcttcc ttgagagttc
      361 tatcgaaatt tggtatttct tctgggccgc tttaaaatgc ggagcggttc cttgtgtctt
      421 attccccgat ttcggctccg atgccctcgg agcccgctta caatccggtc gggcggattg
      481 gatcattacc gatcgttgga atgaaaagaa tgtcggtgcg attcggtcat gtttctcgct
      541 gaaaaaaata attctgctcg aagaaccctc cggtataccg gacactcgaa tggctttgtt
      601 tcatcgggaa agcacaacca atgactcttt tccgctttat ccggccattg aaaccgatcc
      661 ggcgtttatt gtctttacct cggggtcgac cggatttccg aaagcggttg ttcataccca
      721 tcgatgtgcc gaatcgatat tgcgttccat gcaaaacgtg ctccaggttc agaacgatga
      781 catttattgg tgtaccgcgc atccggcttg gattaccggc accgtatacg gtgtgatcgg
      841 ccctttgatt gccggagtgg aatccgttca atacagcggg aatttccatg cccgacgatg
      901 gatgcccatt cttcaaaatc aggaagtatc tgtttggtat tcggcgccga ccgcttttcg
      961 ttctctgatg actcaaccga ttgaattttt tacaaaattt gatttcagca atttacggga
     1021 aatattcagt atcggcgaac cgctgaatcc cgccgtttat tattggggga aagagatctt
     1081 tcaacgtgaa atccgcgata catggtttca gacggaagcc ggcacgatcc gtattgcgaa
     1141 tcttcccggt aaattaatta aacccggcta catgggcatg ccggtggatg acgcattacc
     1201 cgtcatcctg gacgagcagg ggaaagagtg ttcggcgggt caagtcggga gactttgtct
     1261 caaagccggt tggcaatctt gttttaccga ttatttcggt cagcatgatg catatctctc
     1321 caaatttcaa aacggctatt accaaaccgg agatctggct tttcaggata tggacggata
     1381 tttttgtttt gtcgggcggg atgacgatgt tatcaacact tccggacatt tagtcggccc
     1441 ttttgaagtg gaaagtgtgt tgctcgaaca accggaaatt gccgaagcgg gtgtggtggc
     1501 tgcgccggat ccgctgttgt atgagaaagt agctgcattt attgtattga agacgggagt
     1561 ggaatggaca tccgagctcg agacccgtct ggcagttgcc gtaactaccc gtctctcgac
     1621 ccaagctacg ccgcggcaat tcatcgtcgt caatgcttta ccgaaaaatc cggccgggaa
     1681 aattctgcgt aacgaattaa gaaaaagact ccggtcccaa tcttaaaata attcccccgg
     1741 gcagccggca aaaacggaac ggatactcag aaaaaatcaa aggcgggatc tattttcgct
     1801 ttgcttttac cacaccggcg tacggatttt tgattccgtc tatataatcc gcaatgatac
     1861 ttgctccggc catcgagtgg acaagaccat ttccgccgac accgatcaga tagatccggc
     1921 gcggatcctt ttttgaagga ccgatgaagg gaatatgatc tttggaatcg gcggatagcg
     1981 tgttccaact gtattcgact ttgacatcta aattcggcat caaactctgc acccgttcca
     2041 aaatctcccg atttctttta tctatggcac ctttcttttt ttccggcgag tcagcctttt
     2101 tatccgtttc tcccgcgatc acccgattgt cttccgtcat gcggaaaaat aaataaggag
     2161 gtcgtgtctc gcggaataat acgtgatctt gccagagcgg tcgttttgaa accgaagaag
     2221 caaaagtata agtatttaca actttcattt tcttttttaa ttccgggtag ataaccgaac
     2281 atccggtcgc aaaaacaaca tattttgctt gaattcggtt cccgttcttt gttgttgcta
     2341 tgatttggtc cttatccgtt ttcactttct ttattccggt tttttcgtaa tattgaactc
     2401 cgagagaaac attcagtttc attaatgtct gcaaaaactg atagggattc acttccgcat
     2461 tcatcgacgt ttcgagcgcg gcaggtctgt caataccata aatccgcatc aactcggcat
     2521 gatccaaata ggaaaccgga aaataatgat cggataaggc tttgtattct ttatcgagtt
     2581 cggggacgtc ttctttggcg ctcgcaaaaa agaccccttt tcgcggcatt aatcctccgt
     2641 attgttccat ttttcggctg aggttaatca aatctttcat actgtcaaga cacatttgat
     2701 aaaaaagcac ggcttttttt tctccgattg attcaatcag tcgatataac atctcatcat
     2761 tcaggtactg aaggattccg gaacttgccg aagagctacc catcgctaac tgatctttct
     2821 cacaaacaac aacggaatga ttgcgaccgg ctatttcatg tgcacagaga tttccactca
     2881 ttccgccacc gataatcaaa acatctgttt cgagatcatt ttgaagcgga ttaaaacagg
     2941 caaagggttc aattgtctga ttccagaaca gattccccgc ggataacttt ttcataggcc
     3001 ctccatccga cataaattct attatttata aatcataccc ttcaaataaa aaaaagagca
     3061 acctcttttt gaaaggttgc tcaaaaatat gatcactttt gaaaaaatga atcagcgttg
     3121 tttcttatag gatccgccgc cgctatgtcg acgatcatca tcaccacgat tcccgctctt
     3181 atggtcgctg ttgtgcctgc tgcgatcact gttaccggga cggtgtccgg aagaaggctt
     3241 tttggaatcg gccgcaaccg cttgatccaa agtccacccc tccaaaacgg cttggcggga
     3301 aaggcgaatt ttaccatttt catcgatacc agtcaccatg acggtgatct catcgcccac
     3361 attgacaata tcctccactt tattaactcg ttcggaatcc aactgcgaaa tatggaccat
     3421 gccgtcggta ttgggaagaa tctcaacaaa agcgccaaaa tcggtaacac gcactacttt
     3481 accggtgtag atattgccga cgatcgcggt ttcgatcatg gaaagaatct gatcacgagc
     3541 ggcatccgaa tctcccacat tcgtggcgga aatataaacc aacccgtttt catcgatatc
     3601 cacttttgta ttcgttgctt cctgcaatgt gcgaatattc tttcccccgg ggccgataat
     3661 cgcgccgatc ttatcgatcg gaacctgtac cgtgatcaat cgcggaacat gcggtttcaa
     3721 gtctttgcgg ggagccggaa tcacttccaa aatcttaccc aagataaaat gacgaccttc
     3781 atttgcctga tccaacgctt tacgcattat ttcggtcgta attccgcgga ttttgatatc
     3841 catttggagc gcggtaatcc ctttttcggt tccggcgact ttgaaatcca tatctcccaa
     3901 atggtcttcg gcaccttgaa tatcggaaag aattacataa tcgtcccctt ctttgatcaa
     3961 ccccattgct ataccggtga cgggtgcttt aatcggaacg cccgcatcca tcaatgccaa
     4021 tgtagatcca cagaccgatg ccatcgaact ggaaccgttg gagctcatca cttcggaaac
     4081 caagcgaatg gtataaggaa aatcttcttc ggaagggata acggggacaa gcgcccgttc
     4141 tgccaatgca ccgtgtccga tttcgcgtcg actctgcccg ccgagcctct tcacttcacc
     4201 ggtgcagaac ggcggaaagt tgtagtgatg aatataccgt ttattttcca caggtgagag
     4261 tgaatcaatt tcctgtgcat ctcgcggtgt acccagcgtg gcgctggtca acacctgagt
     4321 ttctccgcgg gtaaataagc cggtaccgtg agctcgcgga taaacatcca cttcacacca
     4381 aatcggacga atttcattca atgcacgccc gtccggtcga agatgtcgat ccaagatacg
     4441 gcttcggatg actttgcggt aagcttcttc gaaagcattt ccatattctg actgcttttc
     4501 cgaatcttcg ccggcaaaaa attcaaccgc atcggctttc aactcggcaa gccgatcagc
     4561 caattcatgt ttgtcatgtg gcgcatccag caaagtctcc atcgattggt tatatttttc
     4621 aaaaaccgta tcgaccgtag tctgcggaat gcaggagcgt ttcacttcgc gcttgggttt
     4681 gcccacttcc gcagccattt tcaattgagc ttcgatcaga ggttggattg cccgatgacc
     4741 ggcttcgatc gctccgacca tcgcctcgtt gctgacttca ttcgacccgg cttctaccat
     4801 caaaatggca tctgccgtgc cggcaattcg gagatccaaa tccgaatctt ccatttgctg
     4861 aaaagtcggg ttaatcacat attcgccgtt gatcaatccg acacggacgg caccgaccgg
     4921 tccgtcccat gggatatccg agatagccag agatgccgaa gcggcattga tcgccaaaat
     4981 atccagttga ttttcaaaat cggcggagag gctatagagg ataacctgaa cttcgtttcg
     5041 aatatcttgt gaaaacaaag gccggatcgg acgatcgacc aatctgccga tcaggattgt
     5101 atcaccggcg ggacgtcctt cacgccggaa aaaagaaccc gggatgcgac ctccggcata
     5161 cattcgttct tcatactcta cggtcaatgg gaagaaatcc tgttcttcat gaacttcgtc
     5221 ccccatcgta gctgcggaaa atacaaccga ttcacctaat tgaatcgtta cggcaccacc
     5281 tgcctgagcc gctaattttc cggtctcaaa cgtgatggtt ttaccgccca cttcggcggt
     5341 atatcttttt acttctggtt tcattcttgt tccatttctt tcaccaggcc agggactgaa
     5401 aaaaaacaag aaaaacatct gctttcccat tctttttttg gtttcagcag atgatttgat
     5461 tctgatcttt tttcaatgcc taaccatggt gaatactgat aaataataaa ggtttatacc
     5521 tcttttttaa aaaagagcag atgatttcaa tccacctgct ctctgactcg ctgattcgtt
     5581 atttaaccct caggcctaat ttttcattta aggccacata acttgcataa tcgttttttc
     5641 gcaggtaagc taacaaccgg cggcgctgac cgaccatttt caacagccca cgacgtgagc
     5701 tctcatcgtt tttgcttgtt ttaagatgat tcgtcaaatc ggcgattctt ttggtaagca
     5761 tggcaatctg cacttccaca gatcctgtat caccttcatg caatccaaaa tttttaaata
     5821 tttctgcttt ttcttccttc gttaaagcca tgacaatgct cctctgatta attttttgca
     5881 tgtctccaaa aattcaagca tttacctgaa ttcggacaag tctttacaaa attctaccat
     5941 taattatcga tctttttata aatcgatgat cccggtaaac tcaattctct ttctgcgaag
     6001 tccttaaaag attttggatc gattcccggg aagattccgg aattttcgga agtaactttc
     6061 gtgcgatccg gcgaataatc tttccgctgt ccggattttc caacatttgg cgaacaaaaa
     6121 aagaggtttc aatttcggat cggctcaata acaaaccgat caattcggtt aaatcgggtt
     6181 gtaagacggc gtcggcatcc ggaagcatct tttccagcgc ggtatagact gccggaagat
     6241 taatgaatgc cggatcttca accaaataaa tcaataattg gatcccgcgt tcccggtttt
     6301 tattgtcggg ttccgcacac cagtcttgag ctgatctcat cagtttttcc ggttgctttt
     6361 gtcggatcag accggctgca ttccgaaaaa agactttcag ttcggaaggc tttaatcctt
     6421 tttgggtcca ttcatttata cgagttaaaa cctgatccat atattcaatc ggaatttgcc
     6481 ccaacattcg ggtcgcaatg gtatgcatct ccaggttcac atcattccat agcgaatcgc
     6541 aaagagtcag tgcagcctga ggttgttccc gaattgtctg ataaagcgct aattccaatt
     6601 catggagaac gagttgagga acatgcagtt ccggtgtctg ataagaagtt aagacaccgg
     6661 agcccgatcg aaaagacata atccgataat gatcgagcaa ttgcgccaat ttatcccgga
     6721 atgattccgg atcggtaaat tcccaaagca gggaagttat ttccttacgt aaatttaaca
     6781 gatttacggc gggcatatcg gggttccctc aattgcatgc gatgttctat caatatgctt
     6841 tggaaaagta aattcttttc tccgcttccg caccgcaata gacacatttt ccttttcccc
     6901 gtattttttc atccagcggc atgcagcggg tggtggcttt tgtctcttct tttattttgg
     6961 cttcgcattc cgccgagccg caccaccaag cttccgccca gccattctca acgatttgtt
     7021 tcagctcatc atatgaatcg gcaaaatgaa tattttgatc tctgaattcc gtagcctttt
     7081 caagcaaaga ctgttgaatt tcattcagca gattttctat tttcagatcc agattctcga
     7141 ttgagagaga gatctttccc gcttttcccg ggatatcccg tctggctgcc ataatcgcat
     7201 gtttctccac atctttcggc ccgatttcca agcgcagagg gacacctttt aattcccatt
     7261 cgttgaattt aaatccggga gttaattcgc ttcgatcatc caaccggtat cgaaaacctt
     7321 ttagttggga ttgcactttt tcgatatatt ccatcacaac cgaacgttct tggtcatttt
     7381 tgtaaatagg cacgataacg atctggtaag gagccaatct gggaggcaaa atcaagcctt
     7441 gatcatcgcc gtgagtcatg ataatcgcgc cgatgaagcg ggttgaaagt ccccacgagg
     7501 tcgtccaaca atattgcaac gtattatttt ggtccaggta tttgatatca aatgccttgg
     7561 caaaattttg gcccaaataa tggctggtac ctgcctgtaa ggcttttgta tcccccatca
     7621 tggcttcgat ggaataagta ttgattgcgc ccgcaaattt ttcggtttcg ctttttctgc
     7681 ctttgatgac cggtacggca gcttcattga tggcgaaatc agcataaaca tccagcatcc
     7741 gtaatgtttc ttcctgcgct tcttccgcgg tcgcatgagc ggtatgtcct tcctgccaat
     7801 aaaactccag tgttcgcaaa aacaaacggg tgcgcatctc ccaacggacg acgtttgccc
     7861 attgattaat caaaaccggc agatcacgat agctcttgat ccattttgaa tacatatagc
     7921 cgataattgt ttcggaagtc gggcggataa ttaacggttc ttccaactct tctcctccgc
     7981 cgattgtgac aaccgccagt tccggtgcga agccctccac gtgttctttt tctttttcaa
     8041 aaaaagactt gggaattaat aacgggaaag cggcattcac atggccggtc tccttaaagc
     8101 gtcgatccag ttcacgctga atattttccc atagcgccca tccgtacggc ttgacgacca
     8161 tgcaaccgcg gaccggtgca taatccgcca aatcagcccg taaaacgacc tggttatacc
     8221 aatcggaata attttcgctc cgtgaagtaa gtttatcctc tgccattttt cttttccgtt
     8281 ttccttcgtt cgaatttcag cccattatac tatcggttca atacatccaa agcgatctgt
     8341 gcagatttac gctgttcctc gtgccatcct tcttcatgcg ccatacggtt taaatgtttc
     8401 agacaacttt cctgttcggc aagtgtaacg gacaaaaatg ttgaaagaat ggttgccctt
     8461 tctcttgaca atattccgtc ccaaaatatt tctatctccg accggctaaa atcgatttcc
     8521 tccgaattca tcgctgttct cctttcttga atgaatccat tttatcattt caccgaatta
     8581 tccaaaggtc aatgcaagcg gtaccctctt aagaacaatt ccgatgaaat gatcgttttc
     8641 tgctaaaaaa tttacaccta ttttcttaga atagcgtata aatagaaagg agggattata
     8701 tataaatgac tgctacaatc gatgattacg gattggtttt ttcgggtgga ggcacacgcg
     8761 gtgcctacga aatcggtgca tggaaggcac tgaatgagtt gggaataaaa atacagggag
     8821 ttgtcggcgc ttcgatcggg gcgatcaacg gggctctttt tcttcaagga gatatcgaca
     8881 aagccgaaaa gatttatcgc ggaattcgag tggaagatat tcttgaaatc gagcaaccga
     8941 ttgatccaaa ccatgatctg ctgagtccgt caaacctcac gggattgata aaagaattaa
     9001 ttcagcaaaa agggttgagt aattccgggt tgaaaaatct tattcttcag gaaatcgatc
     9061 tcgataaaat ttataattcc gatctcgatt atggattggt gactttttca attgattcat
     9121 tgtcaccgat cgaaaaattt aaagaagata ttccgaaaga agagttggtt gactatattg
     9181 tggcttccgc aaattttcct atttttaaac ctcacgtggc cggcggatca cgatatattg
     9241 acggtggaat ttatgacaat atgccgatca atatgctcat taaaaaagga tatcggaaaa
     9301 ttattgtcat tgatttaagc ggtgaaggtt tcactcgaaa aatggaacga aaggacggtg
     9361 tgtacctaaa aatcattcgt gccagtgagg atttgggagg gactttcgaa tttgatcagg
     9421 aaaaaatcac cagaaatatt caattgggat atttggatac tttaaaagct tttcagaaat
     9481 tacaggggca ttcttattat ttcacaccgg aatacttcaa tcatcttctg gaaaatttca
     9541 atttagacat tatctacgga ttggaatcgg ctgcgaaaca ttacggaatc gatcgctatc
     9601 aaatatttaa agatgattcg tttctttctg ctattctgga aaagcatcgg caagcgcgta
     9661 aacaatttaa tgactatcag actgacagta ttatgaatcg cgttatcaaa agtccgtctc
     9721 tgattacaaa aatgatcaac agcggaatgg cgatcgcttg gtttgaagaa atggtcatgt
     9781 cgtggcccgg gcaaaaattt gccgcacttc agcgattttt cggagaatat atgatggcgg
     9841 catatgcaat gatcgaactt caattttata tggatgattt ttcattttga attgcattcg
     9901 agtgtgacaa aaaaagcgca acgaatttcg ttgcgctttt ttgacatgat tgcaattcct
     9961 caaaagagct atttttccgc ttctttcttt tctttttcgc gattggcaat gatctcctga
    10021 gtaatatggg gcggaaccac ttcataattg ctgaattcca tcgtaaagac accacggccg
    10081 ccggtcattg atcgcaactg ggttgtataa cgctgcattt ccgctaaggg aaccaaagct
    10141 gtaacggtac tttcgccctt ttcggtttcc attcccaaaa ttcgggcacg ccgcgtattc
    10201 aaatcaccca tgacatcccc catgtactct tccggaacgg ttatttttac cgtatagatc
    10261 ggttcttcca ataccggacc ggcatcggca aacgctaatt tgaaggcttc ccgtccagcc
    10321 gtttcaaaag caatcggttt tgaatcaacc gggtgttcct tcccgtcaaa tacagaaaca
    10381 taaatgccgt gaatcgggaa accggctaaa attccttctt tcatcgcatt gcggatgcct
    10441 ttttcaatcg ccggcatata acttgcgctg atcgcaccgc cgaaaacatc gttatgcatt
    10501 tcgaactccg catccggatt gggtttaact ttcaaatgaa cctcaccgaa ttgaccggaa
    10561 ccgccggatt gtttcttatg tcgatacatg gcggaggctt ctttcacgat tgtttcctga
    10621 taaggcactc tcggctcttt tgtcaaaaga ttcaccataa atttggactc tgcccgccga
    10681 atcgccacat cgatatgctg atcacccatt ccctggagga tggtttgatt ggtcgccatt
    10741 tcgttatacc atgacaaagt catatcttct tcgcaaagac gagtcaatgt ggggctgatt
    10801 ttcgtggaat ctgcctgtgt cttcgggtaa acggcaaccc ggtaaagggc atgaggatat
    10861 tcgggaaccg ggacggttaa cggattcgct ttatcgctga atgtatcacc ggtcaccgtt
    10921 tcacttaatt tcgctaccga acaaatatca ccggcatgaa ccgtttttac cggaatatta
    10981 tcctttccca tcgggatgtt caaatttccg tagcgctctt caaccgaacg ggtaccgttc
    11041 catattctgg aatcggaagt aagcattccc gaataaaccc gcaaaaaagt ctgcttgccc
    11101 acaaatggat cggctgttgt cttccaaaca taggcaacca acggtcccga atcatcacaa
    11161 gaaatttcta catcatttcc ttttttatct tttacgacaa tcggttttct ttcggcggga
    11221 ctcggcatca ggttgataaa ggcatcgaga atcggagcca ctccgatttc ctgtccgcct
    11281 gcggcacaat aaaccggaac aaaatcgcct tttttaaccg ctttggaaaa tccgcgcaaa
    11341 atttcttcat ccgaaagttc catgctttca aagaactttt ccatcagttc ttcgtcgctt
    11401 tccgctgccg cttccgtcaa tgcggctcgg agttgttgag cttcttccat gtattcggcg
    11461 ggaatatcga cgattttatt ctccgcgccg tcatatgctt tcatcgtcag aatatcgatt
    11521 acgccgcgaa aatcaagttt ttctccccaa ggaatctgga cctgaatcag gcgcatatcc
    11581 gtataatccg caagagatgc cattgctttt ttaaaatcag cgttctcccg attcatctta
    11641 ttaacgacta cgatccgcgg caaattaaat ttattaacgt atttccaagc gatttccgtt
    11701 ccgacttcac ggcccgatac ggaatccaaa aggacacaag cgccgtcgga aacgctcaaa
    11761 gcggatacga cttcgccgat gaaatcattg tagccgggag tgtccagaac attaattttt
    11821 ttatctttat attcaatagg gattaccgta ctgtataacg aaatcgtacg tcgtttttcc
    11881 tcatcatcaa aatccgaaac ggttgttccg tcttcaatct ttcccaatcg ggtggttgct
    11941 cctgtaaaat gcaggaaagc ttcagtcatc atggtctttc cggcgctgct gtgtgaaacc
    12001 agcgcaatgt ttcgaatcga atcggtcgaa tattctttca ttgtttattc cttctgaaat
    12061 tattcatggc atcttgccaa caatatacag tttagccaat caattttatt aggttatcgg
    12121 ataattgtca aaggacgatt atgattcaag attccatccg gttcttttcg ggaaaataaa
    12181 aaaagcacag gcttttgatc gatgcctgtg tttttcgttt ttcttttaat aagtttttat
    12241 gaaaccaaat gcatcttcac caatctattt ggattccgtc ccattttggg tcgacaatgc
    12301 cggttttttc ccaaagctca gcagcagatt caaaacgata ccgaaaattg ccgctcccgc
    12361 aatgcaaggt acctgtattc cgaaaaacgg aatactaccg ccgaaggcat actgtccgcc
    12421 gactccgatt accataatcg atgcgatcag tgcgatactc tgtgcgtcaa agaaatcgac
    12481 cttcttatcg accatgatcg caatcccctg tgcggcgatc gccccgaaca agtagatctc
    12541 gagcccgccg attaccggtt gtggaatggc atagattgcg ttgatcaacg gacggaagca
    12601 agagataatc atggcgataa tcgatgcggc aattaagacc ggaaccgaaa aaacccgtgt
    12661 gatcgtcata gcgctgatat tttcaccgta gttcgtcccg gcaggaccgc cgacgagcgc
    12721 cgaaacgata tcggcaattc cgtcacccat caaattcaag cccagtttgt ccgctatgtt
    12781 ataacgcttc tcggattttt tcacctttgc caggttattg acataaatat ccaactgata
    12841 cagatgagcc gttgattccg gtatggttgc gatcgcgatc ggcatgatcg ccgcaaccgc
    12901 cgccaaagag ggtttgggca gtgtaaaaac cggcagggca aataatccgg tcccgttccc
    12961 ttccgaaagc gtccggaaga aatcaaagcc ggtaaatttt gaaatcagga cagtgaccag
    13021 gcaaccgaca atcgggccga aaagcaaggt taattgaccg gcgaaccctt tcagataaac
    13081 ggtaaaaaat attgtggaga gtaaggtaat caaagaaatt gtccaggcat aattacgtac
    13141 caaatcgatc gattcttccg cgactcccgc aggcaccgca cacgcatcca gcaaggcatt
    13201 tgctgccaaa gtcaatccga tagacatcgc gatcgggccg gtgattgttg ccggtaacac
    13261 tttttcaatc gattccgttc cgaatcgttt gataatcaag cccgcaaaaa tcgaaacaaa
    13321 accggacatg ataattccga actgtgcggt ctggatctga gtgttcaaat ccgctccctc
    13381 cgcagccttt gccgccataa ttccggcgat cgccgagatg taagaaaaac tggatccgta
    13441 atacagcgga atctttcttc cggttaccag aataaaacaa aacgtcgcaa gaccgctggc
    13501 aaaaatggtc gttgaaatat gaaaacccgt tataatcgca acggttaccg tagcgggaaa
    13561 cataacaata acttgttgta aagcatacaa aacaagttta aaaaacgggg gacgttcatc
    13621 cggcaaatag ccgatcgact gaacttcatt ttgcgacata ggactctcct cagaaccttg
    13681 aaatttattg accatgattc gaataatttt cggaatcatg gatttcgaca ttggttcggt
    13741 tatcaaattc cggtaaatta accgctatca actcgttcct ggacgtcggt atattcttgc
    13801 cgacataatc cggtcgaatc ggtaattccc gatgtccccg atcaatcaat acagccagtt
    13861 ggatggaagc cggtcttccc agcgcgaaca atgcatcaat ggcggctcgc accgttctgc
    13921 ccgtataaat aacatcatca accaatacaa tattcaaacc ggtgacggaa aaatttattt
    13981 cggaagcgtt taccgaaggc atttccgcta tagacgacag atcatcgcga taaagcgtta
    14041 tatccagact gcctaccgga atctgtaccc cttcgatgat ccggatatct tccgctattc
    14101 tctttgccaa agggacgccg cgacgtttga ttccgatcaa ccccaattta tccgttccgt
    14161 ggttgtgttc aataatttca tgagaaatcc gcatcagagc ccgattcatt gcggtttcat
    14221 ccatgacaac agctttaatg gatggcattt ttgtattcct ttatcagcat tcggattaaa
    14281 aaaaacttgt caacgatttt ctcgtttgac aagttttcct tttttatatt ctttggatca
    14341 gtcatttttt caccttggcg atctcgctgt atcgcattta aagattgaat tggtctgagt
    14401 atatcatgct ttagaaaagg aaaagcaaaa aaagatgatt atcggcagaa aaagttattt
    14461 atttcctcaa atatcaagcg gtcttacaaa ggactcaacc gatgatttta ttttttttaa
    14521 atgggggcgt gtcaaaaatc ataaaaatct gcaacaaatg tcatattttc ataaaacagg
    14581 gcaagaatgt ttccacaaaa tcaagaaata agattattat ttaaaacctt aataataagt
    14641 atttttttaa tttctatccg gcggatttgc tggcggatta ttgatcaatc gaatattttt
    14701 ttgtctgatt atagaccctt atcaaccgac aagaagaaga caagagctct ggatttctat
    14761 ttatcttcgt ttttgtcgat caaaaccgat tagcggataa acatggtgaa atttcaccat
    14821 ttgatcaaaa taccatcata tttggaggaa acacaatgaa aaaaacctta cttactgttt
    14881 tggtactcgt catgctcgtg acgtcgttag tcggctttgc accggcgaaa gaggatgact
    14941 ttgttatcgg tattcttacc ggtaccgttt cccaagggga agaggaattt cgtgccgccg
    15001 aaaaaatggc tgcaaaatac cccgggaaag tgatcacagc gacctaccct gataacttca
    15061 atgacgaagt cgaaacaacg attgcacggg ctctcgaatt ggtatccaat ggcgcagatc
    15121 taatcgtgtt tgtccaagct gttccgggtg ccgcagccgc catcgataaa gtgcatgaac
    15181 tcaaacccga ttttccgttt atagccggtg ttgccggtga agatcccgat gtcatcgctt
    15241 ccaaagcgga cgttgttttg ggcgtcgcgg aaattcaaat gggcacgaag atggctgaac
    15301 aggctgccgc gatgggcgca aaaacatttg ttcattactc ttttgcacgg catatgagtt
    15361 atgcgacaat cgtcgcccgg catgaaattt tgaaaaagag atgcgaagaa ttggggatca
    15421 ctttcgtgga tgccactgct ccggatccga ccggtgacgc aggtgttacg ggtgcacaac
    15481 aatttatcct tgaagactta ccgcgccagg ttgaaaaata cggaaaggaa accgctttct
    15541 tcaacactaa ctgcggaatg cagccggcaa tgattcaggc ggttttagct accggtgctt
    15601 actacccgca acaatgctgc ccctctccgt atcatggtta tccggaagcg ctcggaattt
    15661 ctattccgga agataaacaa ggggatgtat cctatattat cgaacagatt aaggcaaaag
    15721 tggctgaagc ggggatgacc ggtcatttct caacttggcc tgtcccggtc aacatgatga
    15781 tggtcgaagc cggtgtcgaa tatgggttcg attatctgaa cggtgaaatc gataatctgg
    15841 ccgacgtcga tcattggacc gatttattca tggacatcgc aaacggttcg aaggttgaag
    15901 tattcccgct tgaattgaac ggcgttgttt acgacaacta tctgcaaatc ctttcggact
    15961 acattacttt ttaatctgaa tttttctgtt cccgagaaaa atcgaaggat atgaaaaaat
    16021 gaaggagagt ctatccaacc atgaaatcgg ttggatagac tcaagtgtga atcggatttt
    16081 ttccgaaatc ttttttaatg gagataagaa tattttgttt gttcataagt caaatccttt
    16141 ttttattctc aaaactaaag aaatgctttg atttatgaat tttttcatga cacaaattgc
    16201 ctcggaggta taaattggat tctgaatatg tcttgaaaat ggaaaacatc aataaggagt
    16261 attatggcaa taaagtgctg aagaatgttt ccctgaatgt gaaacgcggt gaaattcatg
    16321 ctctcgtcgg tgaaaacggt gcgggaaaat ccacccttat gaatatcttg ttcgggatgc
    16381 ccgtaatcca ttcgaccggc ggatttgaag gaaaggtctt gattgacgga aatccggcgg
    16441 ttatcaattc cccattggat gcgatgcaag ccggtatcgg aatggtacac caggaattta
    16501 tgctgattcc gagttttaca atcgtggaaa atatcaagat cggaagagag atttgtacac
    16561 cgaactttat cagtaaaatt ctccatgata aaaatctgga aacattagac atggaaaaaa
    16621 tggccgcgga tgcaagagcc gcgctgaatc atgtcggaat ggatatcgat gagtataccc
    16681 gtgtggccgg actccccatc ggctaccaac aatttatcga aattgccaga gagatcgata
    16741 aaaccggaat caaattattg gtgtttgatg aaccgactgc cgtattagcc gaatcggaag
    16801 ccgaaagatt gctggaagtg atgcgaaaaa ttacatcctt cggaatcgca atcattttca
    16861 ttacccatcg attggaagaa gtgatggcgg ttgccgatac tatcacggta ctgcgtgatg
    16921 gtgaggttgt aggaatacgt aaaaacaatg aaacttccgt tatcgaatta gcgaaaatga
    16981 tgatcggccg gcacattgaa agtatggccg gcaaagaaca aaccggacga aatttaagct
    17041 ttgaccggat agcgttggaa acaaagaatt tttatgtcga tatgcccggt gaggaagtga
    17101 aaggcgtcga cctgaaagtt cgtgaaggcg aaattttcgg aatcggcgga ttggccggac
    17161 aggggaaagt gggtatttcc aacggcatta tggggatgtt ccccgctgcc ggtgaagtga
    17221 ttatcttcgg aaaaaatgtg agatattctg taatcggaga aactctgaaa catcatacgg
    17281 cttttgtatc cgaagatcgc cggggagtcg ggttgctttt ggatgattcc atcgcattaa
    17341 atatcatttt ctccgcgatg caaacccaaa accgtttcct cagaaaattc ggaccgcttt
    17401 ctcagattaa cagcttcgag gtgcagtcat ttgcgaaaga aatgattcat aagcttgata
    17461 ttcgctgtac cggtcccgat caagcggtgg gttctttatc cgggggaaat caacagaaag
    17521 tctgcttggc gagagctttg accctgaacc ctcagatcct ttttgtatcc gagccgacgc
    17581 gcgggattga catcggcgca aaaaaattgg tgttggacga gttattaaaa ctaaacagag
    17641 agaaaggcgt taccatcgtt atggtttcca gtgaattggt tgaattgcga tcgatttgcg
    17701 atcgaatagc aatcgtttcg gacgggaaag tattcaagat tctcaatccg gatgcaagcg
    17761 atattgaatt cgggctggca atgtccggaa cgctggcgga ggaggaattg gcaaatggct
    17821 gatcgtctca aatcaattta tcattctttc ggcttacctc gaatcattat tattctgttt
    17881 ttcttggctt tattgatcgc cggaggtttg cttgaaattc ccgttccgtc attaatcagc
    17941 gatgttctgg tccgcttcgg aatgaacgga attttggttc ttgcaatggt tccgggaatt
    18001 caagccggaa tcggattaaa cttcggtctt tcgctcggca tcgtagcagg cctgttaggc
    18061 ggcgttatca gtatcgagtc cggtttttcg ggttttgccg gtttagctgt ggcgatcgga
    18121 atcggcattc tgctttccat cgtcatcgga atcggatatg gtctgttgct caattccatt
    18181 aaaggatcgg aaatgaccgt ctcgacatac gtcggttttt cggcgattgc gttgatgaac
    18241 attttttggc tgattctgcc gtttcacaat ccggatatgg tctgggcgat cgggaagggg
    18301 cttcgtgcca caatcgatct cagcagccgg tataaacata ttttaaatga gtttatgact
    18361 ttcaatattc caacttccgc gggcgatatt gtggttccga ccggactgat tttattcttt
    18421 tttatcagtt gcttcattgt atggttgttt ttgcgatcca aatccggtat tgccatgtct
    18481 gcggctggcg gaaatccgaa atttgcatta gccgcaggaa tcgatgagaa taagacgcgt
    18541 attttaggaa cgactatctc taccgtatta tcagcggtcg gcataattgt atatgcacaa
    18601 agttacggtt tttatcaaat ctataacgca ccgctgatga tgagttttgc ggctgcagcc
    18661 ggtgttctga tcggtggggc aagcatcagt cgcgctaaga tttcgcatgt cattatcggg
    18721 acgttccttt tccaagggtt actggtactc gggttgccgg tggcgaataa attattgccg
    18781 gaagggaacc tttccgaagt gatgagaatc atcgtttcaa acggcatcat tttgtatgct
    18841 ttaactaaat cacagggaga taaataatga caaaatcaac tgcgcgaaaa atcacttcct
    18901 tcctgttcgc atataaagta ccggtactgt tcattcttct gagtatcgga ggaatactcg
    18961 tctccggaca atctttgaat ttcatattga tggaattggc ttcccgtttc ggacgggata
    19021 cttttttggt actggcttta attattccgg tcgtcagcgg aatgggactc aacttcagta
    19081 ttgtcgtcgg cgcgatggct gcccaaatcg ccattttttg ggtaatgcac ttattgcaga
    19141 cggttattcc gacttgcccg ccggtgctcg catttttact gtgtgtgctg atcagtacgc
    19201 ccattgcttc cctcttcgga tatcttgccg gaaaattatt caatatgatg aaaggggctg
    19261 aaatgatcgg cggtctcatt ttgggatatt tttccgacgg tctctatcaa cttttatttc
    19321 tgtttatttt tggcggatta atccccattg ccaataaaac attgatgatt gccaccggta
    19381 tcggggtaaa aaataccatt gacttatcca gtaccaaaaa ggtgaacgga ttgaaatatg
    19441 ctatcgataa tatttggaaa gtccctttga ccgaattcct tcagctggta tttgccggag
    19501 ttgtccttta tgcaatcggt gctttcctct atggaaaaat aaaaaatcga aaagtgctca
    19561 gcggtcgttt ttggatcctt ttcggaatca gtgcgatatt attcggcgtt tcttttattc
    19621 ccgctttcat tcaattttcg atgattatca gtatcccggt cgtcacttac atgttaatag
    19681 cggttttttg cctatttatt caatggtttt tgaaaaccag attggggcaa aatatgcggg
    19741 ctgtcggtca gaacaaagtt gttgccaatg ctgccggaat caatgttgat aaagtacgga
    19801 ttatctcgat cattatatcc acggtaatgg cggcgttcgg tcaattgatc tatttacaga
    19861 atatcggggt tttcactact tacggagcac acaaaaatgt ggccctctat tccattgccg
    19921 cgcttttagt cggcggtgct tcggtttaca aagcaaccat cggtcaggca attctcggca
    19981 ttatcctgtt ccatattctg tttatcgtat ctccgttagc cggacagaca ttaatgaatg
    20041 atgcaatgat cggcgaatat ttccgtgtat ttatctgcta tggtgtcata gccatgtccc
    20101 tggcaatgca tgcttggacg atgaaaaata caaaagagaa aaaggtgatc gaaccgatcg
    20161 cattggaaag tgaagtaaag gataactgag ataagcaccg cttttgatat tcaaacgtgc
    20221 ttggaaaagg ctttggtgat ctcaacggat tgccaaagcc tttttgcaag aaaaaaaacc
    20281 gatcttttta ttcggtctcc tattcaaatc gagcctgatt gaccgaaatt tttttcactt
    20341 ggttacggtt tataattaag gtactccctg atgtttgaat cggagaaagt tttcaaaatt
    20401 ccggagggaa aaataaaatg gaaacaatct ttgctccgtg gcggggcgaa tatatcaacg
    20461 gtatcggaaa tcatgcaaag aatccggaca acccgcaaga atgtgttttt tgctcagcca
    20521 taaaagcaac cgacgatttt gaacgataca tcatttacag gggaatcaat gcctttgtga
    20581 ttttgaatct ttatccgtac aacaacggac atatgttgat tattccttat aaccatgtta
    20641 gtgatttatc gcaactcaat accgaaactc accatgaaat gatggatctg atcgttaaag
    20701 cgcagaaagt ccttcaggat gtatatcatc cgcatggaat taatatcggg gcgaatatcg
    20761 gaacggctgc cggagccggc atcgaccaac atcttcattt tcatattttg cctcgttgga
    20821 ctgccgatgt gaattttatg acgacaatcg gccagactcg ggtcattccg gaaaaattgg
    20881 atgaaacgtg gaaaaaaatt cgtgcatctt ggcaaaagat cgattgaaga aatggttatt
    20941 ttatgattct gtcatattaa aataagatga aaaacaattg acgaatcttc tttaggtcag
    21001 gtatagtata gaatcaaagc gttttttcaa aatatcttgc aatagactgg gggaagaata
    21061 agaaatggat tattcgatga tcagtaaact ggaaaaagca aaaagatatg ccaaagagcg
    21121 tgatcgtttc aagattcatt ctctcgaagc aaccgttgag ggatcaaacg gacctcatgt
    21181 cgtaaaatat aaggatggga aatgggaatg tgattgcagt ttttttcagg ggaggggtcg
    21241 ttgcagccat acgatgtccc ttgaaattat tctcgaaggc atggttgata tcgcttacga
    21301 ggatccgcaa taaaaattgg tgaatatagg cgttacaagc ctgaatccat acgggctttt
    21361 ttgacatgta aatattccta aattgttttt gctacaacaa tagcaaacca gaaaatatta
    21421 aggaggaaag aaattatgaa aggtagcgaa aaagtaattc aagctttgaa tgcgaattta
    21481 aaagatgaaa tgactgcgat cgatcaatat atattacatg ccgaaatgtg tgaagattgg
    21541 gggtattcaa aactccatga cagtattgaa aaacgtgcaa tcaccgaaat gaaacacgcg
    21601 gaagcactca ttgcaagaat ccttttcttg gagggaattc cgattatcag tgaactcggc
    21661 aaaatgtcaa tcgggaaaga tgttgaggaa atgatggctt ttgaccataa agccgaacac
    21721 gatgccattc aatcttacaa taatgccatc aaggtttgtg tcgaagaaaa agacaacggt
    21781 acaaaagagt tgtttgaagc cacactaaaa gatgaagaag atcatattga ttggattgaa
    21841 tctcaaatcg atcaaatcag tcaaatggga attcagaatt acttggcaaa acaagtgtaa
    21901 agttgcgatt cggaggttat ctgtttccct tcgaaaaatc cgtaatgcag ggaattataa
    21961 taataaacaa gtctttgact cgatcaaaga cttgttttac aatttgtata ggattttgaa
    22021 aatcgcagtt tgt
//
LOCUS       DAOSMM010000035        21923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2374_length_21923_cov_17.931407, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000035 DAOSMM010000000
VERSION     DAOSMM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21923
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2374_length_21923_cov_17.931407"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    1476..1575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1842..2690
                     /locus_tag="PK898_06345"
     CDS             1842..2690
                     /locus_tag="PK898_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU19690.1"
                     /translation="MIGIITDSTCDIPESLIKQYGIVVLPQMIIWDNQQYRDRIDMEP
                     EEFYQRLISDPERPTSSQVGIPDFKQAYEKLAEQGASEIIVLTVSGAMSGTYQNAVQA
                     AKLVNIPVHVVDSKGPTMSLGWQVLAAARAREEGMDIKGILNRINQVRERLVQYISLE
                     SLEYLQKGGRIGGAIKWVGSKLQVKPLVSVNLQSGLVEAVGLARTHRHVVEMLYKKFF
                     DGLKSYSNLHIAVLHGNVATEAEALAKKIQDEFHPVELIVNITGPVLGLNTGPGALAL
                     CGYAED"
     gene            2898..6836
                     /locus_tag="PK898_06350"
     CDS             2898..6836
                     /locus_tag="PK898_06350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOU19691.1"
                     /translation="MTMQYATKSYSRIPNATEMPDLIEIQLDSFNRLKKEGIAELLDE
                     ISPIESFNKGMKLYFPSKDPISEEWGLKFWFGEPKHSVEECVERDLTFSTPLYVTVLL
                     AGADVSEPLKQDIFLGDFPEMTENGTFIINGSERVVVSQLIRSPGVYFEAPVDRTTGH
                     RLAMAKLIPDRGAWMEFETRKNDYLILKFNRKRTVPITIFLRALAEVDDGIMGDSPLK
                     NGSDEEIMALFADVDNNPDRMYIPSTFKQEPDWEKDYPNMSISQAALLEFFKRMRPGD
                     PVTVENAKQYVEEQLFDQRHYDLERVGRYKLNQKLDLTEYIPNNHRNVTKWDIVRLVR
                     HLIMINNGMVEKDDIDHLGNRRVKTVGELISNKLRIGLRRMERVIKERMSIRDQERVT
                     PVSLVNIRPVVAALREFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFD
                     VRDVHHTHYGRICPIETPEGPNIGLIGRLANFAKVNEYGFIETPYRRVYRNLPKTDDR
                     LVGRQVTETLTAKNGKVIIEKGQRITEEMIPEIRKLDQSVIFITPYISDDVEYLSADA
                     EDKFIIAQSNTRINEWHEFDEKSIASRHYSDFTTYPPESIDYMDVSPQQVVGVSAALI
                     PFLEHDDANRALMGSNMQAQAVPLVNPDIPLVSTGMEKYAAVDSGQVLTSQEDGEVVS
                     VNATQIIVRDNNGMLHTHQLRKYQRSNQSTCIDQRPAVIKGQKVKKNEVLADSSSTVE
                     GQLALGQNMVVAFVSWEGGNFEDAILISERVVMDDKFSSVHIEKYELEARDTKLGPEE
                     ITRDIPNVGDDAIKDLDENGIIRIGAEVGPNDILVGKITPKGEKELTPEERLLRAIFG
                     EKSRDVKDTSLKMPHGERGKVVDVKVFTREENSDLSAGVDTMVRVYVAQRRKLTAGDK
                     MAGRHGNKGVVSRVVPIEDMPYLEDGTPVDIILNPLGVPGRMNIGQVLEVHLGWAAKE
                     LGFRAITPVFDGATEYEIESELARAWMLDKAWKDIENRAWDWLKKTDYDPEQVVDDNE
                     VRMMYISNWLNGKPYDFYKMSLNEFYAKHVVFAEWLREKGYDPDSIIDWENVQPNAPT
                     VLPKDQNAISACLRIWIEEQGEKVAGETEQELRDQSAKISLKTGIPVPIVGKQKLMNA
                     KSGEPFDQPVTVGVMTMLKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEM
                     EVWALEAYGAAYTLQEMLTVKSDDIQGRVSTYEAIVKGKPIQEPGLPASFRVLVKELQ
                     SLGLAVEALLDTGETVRFGKEEERMKTPHMESGLLDAGKETPINLSDLLN"
     gene            7127..8350
                     /locus_tag="PK898_06355"
     CDS             7127..8350
                     /locus_tag="PK898_06355"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOU19692.1"
                     /translation="MSDQIKKVVLAYSGGLDTSAIIPWLIDTYHCEVICACADVGQEE
                     ELNGLEERAKASGASKLYVKDLTDELCENYIWPMIKSGAVYEKKYYLGTSIARPIVAK
                     WLVEIAEKEGADAVAHGCTGKGNDQVRFENTVAALNPNLKVIAPWRIWDFTSREDLLK
                     YLDSKHIPYPDSAKNPKLFSRDRNIWHISHEGGPIENIETEPDESFWVLTVSPEKAPD
                     KPEYVEIEFDAGAPVAVNGERMKPKDIVKTLNKIGGKNGIGRCDMVENRLVGMKSHGL
                     YETPGGTILNYAHSELESITLDRDSQHYKEVVASRYAELIYNGQWFTPLCAAMNKFFE
                     EIDKTTTGTVKLKLYKGNVIPAGRKSKYSLYSEKMASFGDVSLYDHKDASGFIRCFTL
                     PNRVRAMMEQQIENK"
     gene            8420..9805
                     /gene="argH"
                     /locus_tag="PK898_06360"
     CDS             8420..9805
                     /gene="argH"
                     /locus_tag="PK898_06360"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOU19693.1"
                     /translation="MKEQTLWSGGRLDSEMDREMIQLNNSLWIDQRMAKEDVQGSLAW
                     ANAIFEAGVLSRSEYDSIISGLKQISEEFSNDSFQFKSSDEDIHTAVERRLTEIIGPA
                     GGKIHTGRSRNDQVVTDFRLWIKNHISEMKSGIETLQNAFLDRAEQDMGVILPGYTHF
                     QQAQPILLSHWWLSHFWALERDKQRLTEAQSHADISPLGCGALAGSTLPIDRFKLAKD
                     LGFSEPAPNSLDAVSDRDFVCDFLYEMAMIAVHMSRCAEALILYSTTEFGFITLSDRY
                     STGSSLMPQKKNPDSLELIRGKCGSQIGLLQAMLSTLKGLPSAYDKDLQEDKVLFFQS
                     WDNTLLLMKVLTGVIDTLHVNADRCRQAVDTQAFATDVADYLVKKGVPFREAHHIVGK
                     VVKRAEAKHLKLTELSLADWKEISSLFDNDLFRIFSLESAVNARAVWGGTASEQVLKQ
                     LAYAKSKMNTI"
     gene            complement(9893..11509)
                     /gene="rny"
                     /locus_tag="PK898_06365"
     CDS             complement(9893..11509)
                     /gene="rny"
                     /locus_tag="PK898_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOU19694.1"
                     /translation="MYGNFYPGLGDNGFGFLSVSAQTRPPETGSNLLIIILAVVIGLL
                     LVVTATFFITRYLNTKFMKSQQLDAENIILVSRQKAQEIEEEARENANQIAKDAETEI
                     KRRRGELTREEDRLQKRRAEIDARIEKLEQREANLNKRQSNLDKRSNEIDKMAAKQLE
                     ELQKIAQMTTEEARQELLNMVEKDSRNDMARIIRQIEQEARMDGEKKAREIIATAIQR
                     IASDQVSEVTTSIVNLPGEEMKGRIVGRNGRNIRAFEQAAGVDVIVDDTPEAVMISCF
                     DPIRREVARRALDRLILDGRIHPANIEKTLNEEEKEVQKLIIEAGEQAAFEAGVVGLH
                     PEIIKMLGRLKYRTSYGQNQLSHSVEVAKLSSVIASELGANVELARLGALLHDLGKAM
                     DHNTEGTHAKIGAEFAKRYGVNPKAINIIESHHHEVDQESVEAVIVEAADAISGARPG
                     ARREDLELYIKRLRALEEIANSHKGVQNSYAIQAGREIRIIVTPEEVDDLEATYLARD
                     IAHQVEETMQYPGQIKVTVIRETRAVDYAK"
     gene            complement(11526..12161)
                     /locus_tag="PK898_06370"
     CDS             complement(11526..12161)
                     /locus_tag="PK898_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOU19695.1"
                     /translation="MITKLSIQKRNPGRVNVYLDGKFAFGIPKDAAAGLKTGQELTES
                     EIEKLQERDQEDRALSSALRSLNRRLQSESELQKKLIQKGFSPDQIESVMERLKENHL
                     IDDNSFSAMWVENQTAFRPRSKRMLTLELKRKGVSEENIRESLKDLDEEKAALDCGLR
                     YVKRLQGLDRPNFQKKLSAYLARRGFGYELIDQTVHTLWADIQNSEKDSIL"
     gene            complement(12599..14698)
                     /locus_tag="PK898_06375"
     CDS             complement(12599..14698)
                     /locus_tag="PK898_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOU19696.1"
                     /translation="MNFVENGIYLNFDVAADGTLKLYHFGSRPFDPNTILDESQKTGF
                     RVVEVQMSGIDRPEERHGTKFIVTAPGYRLKYQRHSDTRNSFGRKLEFVLIDHETGTV
                     VTCHYQFFDGISVVRCWTEVENRGTENQTLEYVSSFALTGIGKEGLKSRDEKLTLWIP
                     HNSWQREIQWTKHKLPELGLCLNQPEKISRSSKTIGVMNSGNWSTKEYIPMGFLENTE
                     TGDSLIWQIEHNGSWRWEISDQNGHLYLQLSGPNETWSHWSKVLKPGDKFTSVPVAVG
                     TVTGDFEKAIAEMTSYRRVIRRPNEDNEKLAIIFNDYMHCLWSNPTTEKEIPLIDAAA
                     EMGCEYYCIDAGWYSPGFWWDSVGEWQVSLERFPGGLTEVMDYIRSKGMIPGIWLEIE
                     VMGIRCRLAEQVPDDWFFVRHGKRVYDRSRFQLDFRNPEVRAFADEVIDRFVNEYHVG
                     YIKMDYNIEPGIGTEVDADSFGDGLLGHERAYLQWIDDIFERYPDLIIENCSSGGLRM
                     DYAMLSRHSIQSTSDMDNDRMVATISANAPSVLTPEQAAVWSFPLAEDDREEVIFNMV
                     NAIMLRVHQSGQITQISPDRKALVKEALDTYKRIRPDIRRAFPFWPLGLSKYSDPWVC
                     MGLRTDRRAYIALWRRNSEQETISLPVSFLAGRSVKANILYPACDPCPYCWNDGTATL
                     SVTLEKPYSARIFCLEF"
     gene            complement(14738..15970)
                     /locus_tag="PK898_06380"
     CDS             complement(14738..15970)
                     /locus_tag="PK898_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19697.1"
                     /translation="MDAFGRIFTVILSAFILIYMSWLYAPRIRNSMTFWPTKTPAPVT
                     PSPTLISTPTITPLASNTPTLMPTETPGPLSEYWIADGESIDPAVPDSPDGVIVLHAD
                     KNAEVNPPLDDVTWTSSEKIASDLGKSTYTEKWFATYSSGWIRWFMDQPLREGLYEIY
                     TMDTMYSSGGSLDFAVSLGDQLLTPLTGSQHVDFMTSQYDPVQSADTWRSIGIYYVGP
                     STEALNVTTSWEDRDEYTIVAVDRLLIVPRRVSDLGMLNSLSVSGTKYVWDDLEAEIT
                     GKDYIISQKEQLAWDDSYQLVLDPKTDVEITFPNREPYPIGNYKLYAWMPASKGGITA
                     TVKFYLDNTVLPSDTGEESVSYTAPKADAGSWIEIGSWTTDKYYERPRKIKLTIVIPE
                     GSKGEFPVDCFALIHNGF"
     gene            15961..16245
                     /locus_tag="PK898_06385"
     CDS             15961..16245
                     /locus_tag="PK898_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19698.1"
                     /translation="MHPFDPFSVPVFSWDYHYAKGSPNFVFADSNIADSADIPKPGRW
                     AAMANFPDCRLEDTDILTGSVSLDYDSVVWDRIGVDSDSASFEFFLGFFL"
     gene            16332..18233
                     /locus_tag="PK898_06390"
     CDS             16332..18233
                     /locus_tag="PK898_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19699.1"
                     /translation="MKHSLRFLIPSGLISAAATLGLYQMLPLMHFNTKLTALLIGFFL
                     AGTFFLFFFIRFLAKKISINHKMIGIFIVASVFLSILITFLFHLSFPQKEIVLPNRKI
                     HIDVFPDQALADKTKIQFLSLYNGYRGISLSDFSTYGDWKRENDQLVLENFQNGDALE
                     FKGKAGRNIHLYFMVGPRSGKIRIDWGDGSSESYDLSSPMNEEDSLRISHDYGPSAGR
                     FELFNFLINLLSVVSFIFALVMLYWVLVYAFVRKRTKAFKTAFIIISLSTVLIRAVSV
                     YTFPLGWDEGTYSRAAMRYADKALSFQWKEIPSITYNHEHPALVKLTFAVPVILDGRP
                     YYQRFGLNTRNNAMLGKEDYTIFTGRIVSAVFSLWTVQALAVLIHPFAAFFFMIHSLA
                     EEFGAQARLEAMPMLFSFLSIWFFSQFLKGMELRQKKGNLKWLILSALFLGMTAASKI
                     IYCVIAFAILAAAIESGVRQRNIWKELFGSLVLFGIIALGSFFIFNPSVWYNPIGRIS
                     MMIGFHENYQAKASDIYPWWQPIVWVSRSVAHHSDKFFSKSPLGKSPEHFFFSADELI
                     FILACIGFFKIPREYRIYFYWFIFGLFFLFIWGTKWVQYACVVTAPLCIAAYFGSKKV
                     SVWLNRINP"
     gene            18359..19624
                     /locus_tag="PK898_06395"
     CDS             18359..19624
                     /locus_tag="PK898_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOU19700.1"
                     /translation="MANITKEDALTYHQLNGVPGKISVVPSKPLETFIDLSLAYTPGV
                     AFPVLEIAEKPETVYDYTDKGNLVAVISNGTAILGLGNRGSLASKPVMEGKGVLFKKF
                     ADIDVFDIEVDTTDKERFIDTVAAIGNTFGGINLEDIKAPECFEIEQRLQQMLSIPVF
                     HDDQHGTAIILGAALLNALEIVNKKIERVKIVINGAGAAGISCARHLIRLGVKRENLF
                     LCDSTGLVYKGRQDHMNPQKEEFANGDHPAKLDEVIRDADVFCGLSTADVLTAEMIKT
                     MAKDPCIFGMANPNPEIKYEIAREARPDAIIATGRSDYPNQINNVLGFPYIFRGALDV
                     RASKINEEMKLAASYALAELAHLPVPDYVLKAYEIPSLEFGRDYIVPKAFDKRAKWKV
                     SAAIAEAAMKSGVAGIQIDLEAYQKRLGK"
     gene            19844..20266
                     /locus_tag="PK898_06400"
     CDS             19844..20266
                     /locus_tag="PK898_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19701.1"
                     /translation="MDQSDQTNREQEQPRQKKHSLVLATVVIFIALAVIASLMIYFNR
                     DHPAGVIKTANYLVAIIFFILFVISIGLTFIIVKVIQRVDYWLGTLPQFTKQANLQIE
                     SLRSKVAGMTDDITEPIITAEAKFRALHDLFKTKKPRS"
     gene            20273..20461
                     /locus_tag="PK898_06405"
     CDS             20273..20461
                     /locus_tag="PK898_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19702.1"
                     /translation="MSDKNWKRNTLLIGAGIGLLTGLLSAYLRIRKAESNNEIITFKA
                     KDTAKIGTAVFDFMKKFL"
     gene            20464..21483
                     /locus_tag="PK898_06410"
     CDS             20464..21483
                     /locus_tag="PK898_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU19703.1"
                     /translation="MPISPYLPIVVVGTLRRDFVIPPGGKPLNDIPGGHLLHTAVHLH
                     HWENRIGLVSRIGPDFPQEWLDHLTELGMDVHGIKRTALPIEHRFFIRYDGNYQKSTL
                     YPLAQYANEGLPFPKELLGYNVQTNKIDSMNAKTDETIIARDIPENYLESTFIHLCPM
                     DYMTHNLLTQHFAQFGPKRITLRAGKGYMVPQFWHAIPLLVNSLTAFITSISGLKSLF
                     SETRETDIWKMAKIISEWGPEYVIIEQDNFSNLLWDRNHQARYRLGPYQNKVVNLIGI
                     HDAFCGGFIGELKKTYDPVKALIAGNVASSIAMEANHPFYALDTIPGLHEARADYLYS
                     KLELL"
     gene            21644..21853
                     /locus_tag="PK898_06415"
     CDS             21644..21853
                     /locus_tag="PK898_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOU19704.1"
                     /translation="MNRLGKFITGAAIGGLIGIGAILLFAPDKRDEWFDEIQEKTQDI
                     LVEFNRAAQEYKEEMEEDLKARKGA"
BASE COUNT         6013 a         4843 c         4854 g         6113 t
ORIGIN      
        1 tttagttagt ctgcgttatg ggatcaagtg gcgtcaagga agtccatttt cccgaatttt
       61 atggttatta aatttaagtc tgcgtcttaa atccctatcg tcaggatgaa atatctaaaa
      121 aaggaacttc caatgaaaca agatgtcaag ataaaaataa ccatcgaaat cgaaaatatc
      181 caaaagaaaa cagccgaatc acggattcgt tcgaaccatt ttgagcaacg gatgatggaa
      241 attctgagat aaatggggga gatggcgggc gaggcggttt tagattcaga tacggaagga
      301 ataagccaag ggactcgaaa ttgttgggtg agagatgcga attttaacga tggaatgcgg
      361 agaagtaaag gtcacgcagc gggtatataa aggaaaaggt ggaaaccggt agttggtcta
      421 ttcgggttgg agaaatatca gcgacgagac gagaaaatga tggagtggaa ttgtgtgata
      481 atggcgcaac caacctgtct gcggtcagcg gaaattaacg attacctgaa caagaaaacg
      541 aaaacttttg attattcctc cctttcacca cctaaaggcc ggaaaagctt tcattcctta
      601 tttcaacctt ttcagaaaga taattcattc ggaacttctt tcgttaatga tttccacaga
      661 aatttgaaac agatactaag aatcgcgttc actggctcgt atctttcgaa tctgttcaga
      721 caactcgtca gagcaatttg gtccaaaacg tctattttgc ttaaggcgtt catcgtcaag
      781 taaacatcct ttgacaatga actcgcgtag tgtttatgcg ggctaaatat ggaactgggt
      841 cgttttaaga ctgttcactc ggttgccaga atcaataatg gaatctaaat tataaagatc
      901 tatattttat gttactgttc gttctccttc tgtttgaact attcaataat ttcgaatagt
      961 tgcttcttct ttcaattcat tgtgttggca agcggcttga tctgctccaa tgtaaacaaa
     1021 ggaccatatt ccttaacggc tgcctcaaac aacttaagac cttgtgaata tgagtatcgc
     1081 gaatgtatct gtttcaaatt cccatggaaa taatttagga aatagtttcc gagtaactta
     1141 tattcctgta aagatcgaaa tatggaatgg aagcttttcc ggcgttcaga ataaaaaaag
     1201 cagcctgctg tccgcaagac tcagcgactt tcgatttctt ccttcttgat tcacgagatt
     1261 ttacctgatc ttttgctttg atctcttgat gcctgaaggt atgatcagcc aattccgtga
     1321 tataagaaag gatagtatcc acaagggatc tcgaccgcca gcctcaagtt ttcatccgaa
     1381 ttgcaacgga tttcccgaca ttcgttgccc aaaaaaaatc ccgattgaat cacgaattca
     1441 gctgatatct tttggctatt tccgatttcg atggtnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnccgat tgaatcacga attcagctga tatcttttgg ctatttccga
     1621 tttcgatggt tatccttatc ttggcttctt ttgtcattgg aagttccttt ctttgttttg
     1681 aattgtaaaa acgaattttt aggacctgcc ttttttgtca tttttacttg actattgaaa
     1741 tttgaaatgc agatccacat actgatttat cgttttatga ttggaataaa agtaaaataa
     1801 atcaataagg aataaaaata ttttgtttca ggagtaaaaa attgatcgga attattacgg
     1861 atagtacatg cgatattccc gaatcgctga taaaacaata tggaatcgtc gttcttcctc
     1921 agatgatcat ttgggacaat caacaatatc gtgatcgaat agatatggag cctgaggaat
     1981 tttaccaacg gttgatctcg gatccggaac gtcccacttc ttctcaagtg gggattcccg
     2041 attttaaaca agcttatgaa aaactcgcag aacagggagc atcggaaatt attgtgctta
     2101 ccgtcagcgg agccatgagc ggtacttatc aaaatgccgt gcaagccgca aagttggtaa
     2161 atattcccgt tcatgtcgta gattccaaag gtccgacgat gtctttgggc tggcaagtgc
     2221 tggcagcggc tcgtgctcgt gaggaaggaa tggatatcaa aggaattctc aaccggatta
     2281 atcaggtaag agaacggttg gtccagtata tcagcctgga gtcgcttgag tatctccaaa
     2341 aaggcgggcg gatcggcgga gcgatcaaat gggtcggcag caagctgcag gtaaaaccat
     2401 tggtttcggt caaccttcaa tccggccttg tggaggcggt cgggttggca cgcacccacc
     2461 gacacgtggt tgagatgctg tataagaaat tctttgacgg attaaaaagt tatagcaacc
     2521 tccacattgc ggttttacac ggcaacgtag ccacggaagc ggaagctctg gcgaaaaaaa
     2581 ttcaggatga gtttcatccg gttgaattga ttgtaaatat taccggtccg gttttaggat
     2641 tgaacaccgg tcccggcgca ttggctttgt gcggctatgc ggaagattaa tatcatcgaa
     2701 taattaatat ctgtttttct ttatcaggca aaaagaaaac ggatctgtca catcatgaaa
     2761 tttcagataa aaattgcagc agaattaatg atgtgataaa ataaactttt gcgttggtaa
     2821 gaatatctac atggcgggtc gttcctatgc gcctgattga tcatctgacc agatgaattt
     2881 attaggaggt tacctatatg accatgcaat acgcaacgaa atcctattca aggattccga
     2941 atgcaactga aatgccggat ttaatcgaaa tccaattgga ttcatttaat cgattaaaaa
     3001 aagaaggcat cgcagaactt cttgatgaaa tttcccctat tgaatctttc aataaaggga
     3061 tgaaacttta ttttccgagt aaggatccca tctccgaaga atggggtttg aaattttggt
     3121 tcggcgaacc gaaacattcc gtggaagaat gcgtcgaacg ggatctcact ttctccactc
     3181 cgctatatgt gacggtattg ttagccggcg cggatgtctc cgaaccgctg aaacaggata
     3241 tatttttagg cgacttcccc gaaatgacag aaaacgggac gtttatcatc aacggctcgg
     3301 aacgggttgt cgtatctcag ttgatccgat ctccgggtgt ctacttcgaa gcacccgtcg
     3361 accgtaccac cgggcaccgg ctggcaatgg caaaattgat tccggatcgc ggcgcctgga
     3421 tggaatttga gaccagaaag aatgattacc tgattttaaa gttcaaccga aaacgaacgg
     3481 taccgattac gattttcctg agggcacttg cagaagtcga tgacgggatc atgggcgatt
     3541 caccgctcaa gaacggttcc gacgaagaaa tcatggctct ttttgccgat gtggataaca
     3601 atccggatcg gatgtacatt ccgtcgacat tcaaacagga accggattgg gaaaaagatt
     3661 atccgaacat gtccatttct caggcagctt tgcttgagtt cttcaaacgg atgcgccccg
     3721 gtgatccggt cacggtagaa aacgcaaaac agtacgttga agagcaactt ttcgatcaac
     3781 gccattacga ccttgaacgg gtcggacgat ataaattaaa tcaaaagctg gatctgaccg
     3841 aatacattcc gaacaatcat cggaatgtta ccaaatggga tatcgtccgt cttgtccgcc
     3901 atttgatcat gatcaacaac ggtatggtcg aaaaagatga tattgatcac ctcggtaacc
     3961 gtcgtgtcaa aacagtcggt gaattgattt ctaataagct gcgcatcgga ttgcgccgaa
     4021 tggaacgggt tatcaaggag cggatgtcca tccgcgatca ggaacgggtt acacctgtca
     4081 gtttagtcaa tattcgaccg gttgtggcag ctttaagaga attcttcggc tcctctcaac
     4141 tttctcagtt catggatcag acgaatccgt tagccgaatt acgccataaa cgaacgcttt
     4201 cggctttggg ccccggtggt ctgcggcggg aacgtgccgg attcgatgtt cgtgatgtcc
     4261 accataccca ttacggccgg atttgtccaa ttgaaactcc cgaaggtccg aacatcggcc
     4321 tgatcggccg gttggcgaat tttgccaaag tcaacgaata cggcttcatc gaaactccat
     4381 accgtcgcgt ctatcggaat ctacccaaga ccgacgacag gttggtcgga cggcaagtca
     4441 cggaaacact gaccgcaaaa aacggaaaag tcattatcga aaaaggtcag cggattacgg
     4501 aagaaatgat tccggaaatt cggaaactcg atcagtccgt gatcttcatt acaccgtata
     4561 tttcggatga tgtcgaatat ctttcagcgg atgcggagga taaattcatc atcgctcagt
     4621 ctaatacccg aatcaatgag tggcatgagt ttgacgaaaa gtcaatcgca agccgccatt
     4681 attccgattt cacaacctat ccgcccgaat ccatcgatta tatggatgtc tctccgcagc
     4741 aggttgtcgg tgtcagcgcg gcgctgattc cgttcttgga acacgacgat gccaaccgtg
     4801 cgctgatggg ttccaacatg caggcgcaag ccgttccgtt ggtaaatccg gacattccgc
     4861 tggtctccac cggaatggaa aaatatgcgg ccgtcgattc cggtcaagtt ttgacttctc
     4921 aggaagacgg cgaagtggtt tcggtcaatg cgacccaaat cattgtccgg gataacaacg
     4981 gaatgctgca tacacaccaa ctgcgaaaat atcaacgttc gaatcagagt acctgtatcg
     5041 atcaacgtcc ggcggtaatc aaggggcaga aagtcaagaa aaatgaagtg ctggccgatt
     5101 cctcttcgac tgtcgaagga caattggctt taggacaaaa tatggtcgtg gctttcgtct
     5161 cctgggaagg cgggaacttt gaagacgcca tcttgatttc cgaaagagtg gtaatggatg
     5221 acaaattctc ttccgttcat attgaaaagt atgaattgga agcccgcgat accaagcttg
     5281 gtcccgaaga aatcacacgg gatattccga atgtgggtga cgatgccatc aaagacctgg
     5341 acgaaaacgg gattatccga atcggtgccg aagtcggtcc caatgatatt ttggtcggga
     5401 aaatcactcc taaaggggag aaagaattaa ctccggaaga gcgtctgctg cgggcgattt
     5461 tcggcgaaaa atcccgtgat gtcaaagata catccctgaa aatgccgcat ggcgaacgcg
     5521 gaaaagttgt ggacgtcaaa gtttttaccc gcgaggaaaa cagtgacctt tccgccggtg
     5581 tggacaccat ggttcgcgtc tatgtcgctc aacgccggaa attaaccgca ggcgataaaa
     5641 tggcgggacg tcacggaaat aaaggcgttg tttctcgggt ggttccgatc gaagatatgc
     5701 cttatttgga agatggcacg ccggtcgata ttatcctcaa tccgttgggt gttcccggac
     5761 gtatgaacat cggacaggta ttggaagttc atttgggatg ggcggcgaaa gaattgggat
     5821 tccgtgccat cactccggta tttgatggcg ctaccgaata cgaaatcgaa tccgagctgg
     5881 ctcgtgcttg gatgctggac aaagcttgga aagatatcga gaatcgtgct tgggattggc
     5941 tgaaaaaaac agattatgat ccggaacagg tcgttgatga caacgaagtc cgtatgatgt
     6001 atatcagtaa ctggttgaac ggaaaacctt atgactttta caaaatgagc ctgaatgagt
     6061 tttacgcaaa gcatgtcgtc tttgccgaat ggctccgaga aaaagggtat gatccggact
     6121 ccatcatcga ctgggaaaat gtgcaaccga atgctccgac cgtattaccg aaggatcaga
     6181 atgccatttc agcttgtttg agaatctgga ttgaagaaca aggggaaaaa gtagccggtg
     6241 aaaccgagca agaactgcga gatcagtccg caaagatcag cctgaaaacc ggaatccctg
     6301 ttccgatcgt cggcaagcag aaattgatga atgcaaaatc cggcgagccc ttcgatcaac
     6361 cggtcacggt gggcgttatg actatgctga aactgcacca cttggttgaa gataaagttc
     6421 atgcccgttc cacaggccca tacagtttgg tgacgcagca accgttggga ggaaaagctc
     6481 aattcggcgg acaacgtttc ggtgaaatgg aagtctgggc actggaagcc tacggcgcgg
     6541 catatacgct gcaagaaatg ttgacggtca agtctgatga tatacaggga cgtgtcagca
     6601 cctatgaagc cattgttaaa ggcaaaccga ttcaggaacc cgggttgccg gcttccttcc
     6661 gtgttctggt caaagagttg cagagcttgg gcttggcggt tgaagccctt ctcgatacag
     6721 gtgaaacggt ccgattcggc aaggaagaag aacggatgaa gactcctcat atggaaagcg
     6781 gtttactgga tgccggaaaa gaaacgccga ttaatttgtc cgacttattg aactgagtca
     6841 atcagaaaac aattcgaaag tccatcgaaa gaaatctcga tggacttttt tgaaataatc
     6901 actattttta taaatttgtc tttttccggt ttcggattca tttataataa cggtcatgaa
     6961 aagtcagcaa ccggtttttt tctatatccg acgactctgc cgcattacag gaatgtattc
     7021 agcggaacgt ttcggttaaa tcatacgatc ggatagacaa aagacctctg ctgaatcggc
     7081 aaaggtcttt tttttgatta aatttatttc gttcaggaga aacaatatgt cagatcaaat
     7141 caaaaaagtc gttttagctt attcaggcgg tttggatact tccgcaataa tcccttggtt
     7201 gattgatacc tatcattgcg aagttatttg cgcttgtgcg gatgtgggac aggaagaaga
     7261 attaaacgga ctggaagagc gggcaaaagc cagcggggct tccaaattat atgtcaaaga
     7321 tctcacggat gaactttgcg aaaactacat ttggccgatg atcaagtcgg gcgctgttta
     7381 tgagaaaaaa tactacctcg ggacttctat agcccgcccg attgtcgcaa aatggttggt
     7441 cgaaattgcc gaaaaagaag gtgccgacgc cgttgctcat ggctgcaccg gtaaaggaaa
     7501 tgatcaggta cgcttcgaga ataccgtggc tgcgctgaat ccgaacctga aagtaatcgc
     7561 accttggcgg atttgggatt ttacctcacg agaagatttg cttaaatatc tcgacagcaa
     7621 acatatccct tatccggatt ccgcaaaaaa tccgaagctc ttcagccggg atcggaatat
     7681 ttggcatatc tcgcatgaag gcggaccgat cgaaaatatc gaaacggagc cggatgaatc
     7741 gttttgggtg ttgaccgtct caccggaaaa agcaccggac aaaccggaat acgtagaaat
     7801 cgagtttgat gccggcgctc cggttgccgt caacggcgaa agaatgaagc cgaaggatat
     7861 tgtaaagaca ctaaacaaaa tcggcgggaa aaacggtatc ggccgctgcg atatggttga
     7921 aaaccgtttg gtcggaatga aatctcacgg gctttatgag acacccgggg ggacaattct
     7981 aaactatgct cattccgaat tggaatcgat cacactggat cgtgattctc agcattataa
     8041 agaagtggtc gcatctcgtt atgcggaatt gatttataat gggcaatggt ttactcctct
     8101 ttgcgccgca atgaacaaat ttttcgaaga gattgataaa acgacaaccg gtacggtcaa
     8161 gttgaaactg tataaaggca acgtcattcc cgccggaaga aaatcgaaat acagtcttta
     8221 cagcgaaaaa atggcttctt tcggtgatgt atccctttac gatcataaag atgccagcgg
     8281 attcattcgt tgctttacac ttcccaacag ggttcgcgca atgatggaac aacagattga
     8341 aaacaagtaa accgaattcc ggcgcatttg aggttgttcg gaaaaataat gatccgaatt
     8401 ttttcgggaa ggaattctca tgaaagagca aacactttgg tccggcggaa gattggacag
     8461 tgaaatggat cgagagatga ttcaactgaa caattcttta tggattgatc aacgaatggc
     8521 gaaagaagat gttcagggaa gtctggcttg ggcaaacgca atttttgaag ccggcgttct
     8581 gagccggtca gaatatgatt caatcatttc cgggttgaaa cagatttccg aagaattttc
     8641 gaacgactct tttcaattca aatcttccga cgaggatatc catacggctg tcgaacgaag
     8701 gctgaccgaa atcatcggac ctgccggcgg aaaaatccat accggacgca gtcgcaatga
     8761 tcaggtggtt accgatttcc gtttatggat caaaaatcat atttccgaaa tgaagtcggg
     8821 aatcgaaact ttacaaaatg cgtttctcga ccgtgccgaa caagacatgg gagtgatcct
     8881 gcccggttat acccattttc agcaagcgca acctatctta ctgagccact ggtggttatc
     8941 ccatttttgg gcgcttgaac gcgataaaca gcgattaacg gaagcgcaga gtcacgcgga
     9001 tatctcaccg ctgggatgtg gggctttggc aggatccacg ctgccaatcg atcgattcaa
     9061 actggcaaag gacttggggt tttccgaacc cgctcccaac agtctggatg ccgtatccga
     9121 tcgggacttt gtctgcgatt ttttatacga aatggcaatg atcgctgtgc atatgagccg
     9181 atgtgcggaa gcgttgattc tctattccac aacggaattc ggttttatca cgttatcgga
     9241 tcgctattcg accggatcca gtctgatgcc gcaaaagaaa aatcccgatt cactcgaact
     9301 cattcgcgga aaatgcggca gccagatcgg cttactgcaa gcgatgcttt caacgttaaa
     9361 aggattgccc tccgcatatg acaaagatct tcaggaagat aaagtcctgt tctttcaatc
     9421 ctgggataac acgttgctgt tgatgaaagt attaaccggc gtgatcgata cacttcatgt
     9481 caatgccgat cgctgccgtc aggctgtcga tactcaggct ttcgcaacgg atgtcgcgga
     9541 ttacctggta aaaaaaggcg tccccttccg ggaagctcat catattgtcg ggaaagtggt
     9601 aaaacgtgcc gaggcgaagc atctgaaact cactgaactc tccttggctg attggaaaga
     9661 aatttcatcc ttattcgaca acgatttgtt ccggattttt tcgttggaat cagccgtcaa
     9721 tgcccgtgcc gtctggggag gaacagccag cgaacaggtg ctgaagcagt tggcttatgc
     9781 gaaaagcaag atgaacacga tttgatttcg agttcgtatg aaagaaaagg atctctcggt
     9841 tagctacatc gaaagatcct ttttatttta atggtcagcg gatttcagtt ttctactttg
     9901 cgtagtccac agcccgcgtt tcacggatga ccgtcacctt tatctgcccc ggatattgca
     9961 tggtttcttc gacttgatgt gcgatatcgc gagccaaata agtggcttcc aaatcgtcta
    10021 cttcctccgg tgtcacaata atgcgaattt cgcgacccgc ttgaattgca taagaatttt
    10081 gtacgccttt atgagaattt gcgatttctt ccaaagcgcg cagccgtttg atataaagct
    10141 caagatcttc tcttctggct cccggtctcg cgcccgaaat cgcatccgcc gcttccacta
    10201 ttactgcctc aacactttcc tgatcaacct catggtgatg agattcgata atattgatcg
    10261 ctttgggatt tacaccgtat cgtttcgcga attccgcccc gatttttgca tgagtccctt
    10321 cggtattatg atccatcgct tttccgagat catgtaacaa tgcgcccaac ctggctaatt
    10381 ccacattggc acccaattcg gatgcgatta ccgatgaaag tttagcgacc tcaaccgaat
    10441 gagacaactg attttgcccg tacgaagtcc gatattttaa tcgacccagc attttaataa
    10501 tttccggatg caagccgacc acaccggctt cgaatgcggc ctgctcaccc gcttcgatga
    10561 tcagcttctg aacttccttt tcttcttcat tcaatgtttt ttcgatgttt gccggatgaa
    10621 tccttccgtc caaaatcaat cgatccaacg ctcgacgcgc cacttccctg cgaatcggat
    10681 caaaacatga aatcatcacc gcttccggtg tgtcatccac aatgacatcc actccggcag
    10741 cctgttcgaa agcacgaata tttcgtccat tcctgccgac aatccgcccc ttcatttctt
    10801 cgcccgggag attgacaatg gaggtcgtca cttcgctgac ctgatcggaa gcgattcttt
    10861 gaatcgcggt ggcaatgatt tcgcgggctt tcttttcacc gtccatacgg gcttcctgtt
    10921 cgatttggcg aataattcga gccatatcat tacgggaatc tttttcaacc atatttaata
    10981 actcttgccg cgcctcttcc gtcgtcatct gagcgatttt ctgaagctct tccaactgct
    11041 ttgcagccat tttatcaatc tcgtttgaac gcttatcaag gttgctttgg cgcttattca
    11101 ggttcgcttc ccgctgctcc aatttttcga tccgggcatc gatttcagcc cggcgttttt
    11161 gaagccggtc ttcttcgcgt gttaattctc ctcgccgccg ctttatttcc gtctcggcat
    11221 ctttggcgat ttgattggca ttttcccgag cttcctcttc aatttcctgt gccttttggc
    11281 ggctgacaag gataatattc tccgcatcaa gctgctgaga cttcataaat ttggtattca
    11341 aatatcgggt gataaagaaa gttgccgtca ccaccaacag caatccgatt acaacggcta
    11401 agattatgat caggaggttg gatccggtct ccggcggtcg ggtttgcgcg gatacagata
    11461 agaatccaaa gccgttatct cctaatcctg gataaaaatt tccatacata gcattttcct
    11521 ttatttcaaa gtatggaatc tttttcggaa ttctggatgt ctgcccaaag agtatgcacc
    11581 gtctgatcga ttaattcata tccgaatcct cttctcgcca aatatgcact cagctttttt
    11641 tggaaattcg gtcggtctaa cccctgtaat cgtttcacgt agcgaaggcc acaatccaac
    11701 gctgcctttt cctcatccag atccttcaaa gattcacgga tattttcttc ggagacgcct
    11761 tttcttttta attcgagagt aagcatccgc ttgcttctcg gacggaacgc agtctgattt
    11821 tcaacccaca tcgcagaaaa agaattgtca tcaatcaggt gattctcttt caatctttcc
    11881 ataaccgatt caatttggtc cgggctgaat cccttctgaa tcagtttttt ctgcagttca
    11941 ctttccgatt gaaggcggcg attgagtgat ctcaaagcgc ttgataaagc ccggtcttcc
    12001 tgatcgcgtt cttgaagttt ttcaatttca ctttctgtca attcctgccc cgttttcaaa
    12061 ccggcggcag catccttggg aattccgaac gcaaacttcc cgtccagata gacattgact
    12121 ctcccgggat ttctcttttg aattgacaac ttcgtaatca cacgtccgtc catataaaaa
    12181 aagccgtcac gacaatattc tctcggacgg cttatttttt taactgaacc gacaggatca
    12241 aattctccga atatcggtaa aacgtagaaa attttatgaa tttttccctt tacatttcat
    12301 tcccatcaat ttcactttga tgatcaggct ttaaattcat tgctttctgt ttttatatca
    12361 gccgtttccg gctgatttca ttccctaaat aagccgtacc atgaatattg cacggtaata
    12421 tccattttca attcgatatt ataccttaaa ataagtaatc tgttaacttt tatccaaatc
    12481 ggaggaattc tcagggaaaa tcaatcggtt ttggatcatc cgaaaggaag tcgtaaggtt
    12541 ttttattcat cttagattcg ctgaataagc cttctctccg atccgaattt tttgtagatc
    12601 aaaattccaa acagaatatc cgcgcggaat aaggcttttc caatgttacg ctgagcgtgg
    12661 ctgttccatc attccagcaa taaggacatg gatcgcaagc cggatataaa atgttcgctt
    12721 tgaccgacct tcctgccaaa aatgaaaccg gtaaagagat cgtttcctgc tcgctgttcc
    12781 tgcgccaaag agcaatatac gcacgacggt ctgttctcaa tcccatacag acccaaggat
    12841 cggaatattt cgaaagtccc agcggccaaa aaggaaatgc ccggcgaata tccggtcgaa
    12901 tccgtttata agtatccaac gcttctttca ccaacgcttt ccgatcggga gaaatttgcg
    12961 taatttgtcc gctttgatga acgcgcaaca ttattgcatt caccatatta aagattactt
    13021 cctcacgatc atcttccgcc agcgggaaag accaaacagc ggcctgttcc ggagttaata
    13081 cagaaggcgc attggcagag atagtcgcaa ccattcgatc attatccata tcactggtgg
    13141 attggatact gtgacgactc aacatagcgt aatccatccg taaaccgccg cttgagcagt
    13201 tctcgataat caaatcggga tagcgctcaa aaatatcgtc aatccactgc aggtatgctc
    13261 tctcatgtcc cagcaatccg tccccgaaac tgtccgcatc gacttccgtg ccgatgcccg
    13321 gttcgatgtt gtaatccatt ttgatataac ccacatggta ttcgtttaca aatcgatcga
    13381 ttacttcatc ggcaaaagca cgcacctccg gattacggaa atccaattgg aatcggcttc
    13441 gatcatatac ccgttttcca tgccgtacaa agaaccaatc atccggtacc tgttcagcca
    13501 aacgacaacg aatccccatt acttcgatct ccagccatat tcccggaatc atccctttgg
    13561 aacggatata atccattacc tcagtaagtc ctcccggaaa tcgttccagg ctgacctgcc
    13621 attccccgac actgtcccac caaaagcccg gagaatacca accggcatcg atacagtaat
    13681 attcgcaccc catttccgcc gccgcatcga ttaaaggaat ttccttttcg gtcgtcgggt
    13741 tggaccacaa gcaatgcata taatcgttaa aaatgattgc aagcttttcg ttatcctcat
    13801 tcggtcgacg aattacccga cggtatgagg tcatttcggc aattgccttt tcaaaatccc
    13861 cggtaacggt cccgactgct accggaacag aggtaaattt atcgcccggt tttaagactt
    13921 tactccaatg agaccaggtt tcattgggac cgctcagttg taaatataaa tgaccgtttt
    13981 ggtcgctgat ttcccaacgc caagaaccgt tatgctcgat ctgccaaatc agactgtcac
    14041 ccgtctcggt attttccaaa aatcccatcg ggatatattc tttggtcgac caattcccgc
    14101 tgttcatcac accgattgtt tttgaggagc ggctgatttt ttcgggctga tttaaacaaa
    14161 gccctaactc aggcagtttg tgctttgtcc actgtatttc tctctgccaa gaattatgcg
    14221 gaatccagag cgtcagtttt tcatcacgac tttttagtcc ttctttcccg atccctgtca
    14281 atgcgaaaga gctcacatat tcaagcgttt gattttcagt tccccggttt tccacttccg
    14341 tccaacaacg tacaacggat ataccgtcga aaaattgata atgacaggtg accacagtac
    14401 cggtttcatg atcgattaaa acgaattcaa gttttctgcc gaaagaatta cgcgtatcgg
    14461 aatgacgttg atatttcaat cgataccccg gtgcggtcac aataaatttg gtgccgtgac
    14521 gttcttcggg acggtcgatt ccggacattt gcacttcgac aacccggaat cctgtttttt
    14581 gactctcatc caaaatcgtg ttcggatcga atggccggga accgaaatga tacagcttca
    14641 aggtgccgtc agctgctaca tcaaaattta aataaattcc attttcaaca aaattcatgg
    14701 gattttctct ctttcatcaa tttcttgaac ggagcgatca aaatccgtta tggatcagtg
    14761 cgaaacaatc taccggaaac tcccctttag aaccttccgg aattacgatg gtcagtttaa
    14821 ttttccgcgg tcgctcataa tacttgtcgg tcgtccaaga accgatttcg atccatgagc
    14881 ctgcatccgc tttcggtgcc gtatacgaga ccgattcctc accggtgtcg gaaggcaaaa
    14941 cggtattatc caggtaaaat tttacagtgg cggttatccc gcccttactt gccggcatcc
    15001 atgcatataa cttatagttt ccgatcggat aaggttccct attggggaag gtaatttcga
    15061 catccgtttt cggatccaga accagctggt aagaatcatc ccaagcgagc tgctcctttt
    15121 gtgaaataat atagtcttta ccggttatct cggcttccag atcatcccaa acatatttcg
    15181 ttccgctgac agaaaggctg tttagcatgc cgagatccga tacacgacgc ggaacaatga
    15241 gtaatcgatc aaccgcaaca atcgtatatt catcccgatc ctcccaagaa gtagtcacat
    15301 tcaatgcttc cgtcgaaggt ccgacataat aaataccgat gctgcgccag gtatcggcgg
    15361 attgaaccgg atcatattga cttgtcataa aatcaacatg ttgcgatccc gttaaaggag
    15421 tcaataactg atctcccaaa ctcacggcaa aatccaacga accacccgaa ctgtacatgg
    15481 tatccatcgt gtatatttca tacaaccctt cccgaagagg ctgatccata aaccaacgta
    15541 tccagccgct ggaataagtc gcaaaccatt tttcggtata ggtagatttt cccaaatcgg
    15601 aagcgatctt ttcggaactc gtccaagtga catcatccaa aggcggattg acctccgcgt
    15661 ttttatccgc atgcaaaacg atgacgccgt cgggagaatc gggaacagcc ggatcgatcg
    15721 attccccatc cgcaatccaa tattcggaaa ggggcccggg cgtttcggtc ggcattagag
    15781 tcggcgtgtt ggaagccaaa ggagtaatcg tcggtgtgga aatcagcgta ggcgaagggg
    15841 taaccggagc cggcgtttta gtcggccaaa aagtcatact gtttcgaatt cgcggagcat
    15901 ataaccagct catataaatc aaaataaaag cggaaaggat cacggtaaaa attcgaccga
    15961 atgcatccat tcgatccttt ttcggtcccg gttttttctt gggattatca ttatgcgaag
    16021 ggttcaccga attttgtttt tgcggattcg aatattgccg attcggcgga tatcccgaaa
    16081 cccggtcgtt gggctgccat ggccaatttc ccggattgcc ggctggagga tacggatatc
    16141 ctgacgggtt cggtttccct tgattatgat tcggttgttt gggatcggat tggggttgat
    16201 tcggattcgg catcttttga attttttctc ggttttttct tataaatcat atcaagaata
    16261 tgtttttcat tcagattccc gataaggatc aaaatgtaat tatatcaatg aactggttat
    16321 aattctccac tatgaagcat tcgttgcgat tcttgattcc ttccggattg atatcagccg
    16381 ccgctacgct cggactttat caaatgctac cgttgatgca tttcaataca aaacttacgg
    16441 cgttgttgat cggttttttc ttagccggta ctttttttct tttctttttc attcgatttc
    16501 ttgcaaaaaa aatatcgatt aatcataaaa tgatcggtat ttttattgtt gcttccgtat
    16561 ttttatcgat tctgattact ttcctttttc atttgagttt tccgcaaaaa gaaattgttt
    16621 taccgaatcg aaaaattcat atcgatgtct tcccggatca ggctttggcg gataaaacca
    16681 aaattcaatt cctctctctc tataacgggt accggggaat ttctttatcg gacttttcga
    16741 catacggaga ttggaaaagg gaaaatgatc agttggtact cgagaatttt caaaacggcg
    16801 acgcacttga attcaaaggg aaagcgggca gaaacattca tctttatttt atggtcggtc
    16861 cccggtccgg aaagatccgg atcgattggg gagacggatc gtcggaatcc tatgatctgt
    16921 caagcccgat gaacgaagaa gattctctcc gaatttctca tgattacggg ccatccgccg
    16981 gaaggtttga attattcaat tttctgatca atttactttc cgtagtctct ttcatattcg
    17041 ctttggtgat gctttattgg gtattggttt acgcattcgt ccggaaacgc acaaaagcgt
    17101 ttaaaaccgc gttcatcatc atttctctct ctaccgtact gatacgtgcg gttagtgtat
    17161 acactttccc gttgggatgg gatgagggaa catattctcg ggccgccatg cgctatgccg
    17221 acaaagctct ctcttttcaa tggaaagaaa tcccctcaat tacctataat catgaacatc
    17281 cggctttggt caaattgaca tttgccgttc ccgtcattct ggacggacgt ccttattatc
    17341 agcgattcgg tttaaatacc cgaaacaatg ctatgttggg aaaagaggat tacacaattt
    17401 ttacggggcg aatagtcagt gccgttttca gcctctggac agtccaagca ttggctgtct
    17461 tgatccaccc tttcgccgct tttttcttta tgatccattc tctggcggaa gaattcggag
    17521 cacaagccag attagaagct atgccgatgc ttttttcttt cctgagtatt tggtttttca
    17581 gtcaattcct aaaaggaatg gaactccgtc agaagaaagg aaatttgaaa tggttaatac
    17641 tttcagctct gtttctcgga atgactgcgg catccaagat tatttattgt gtaattgctt
    17701 tcgccatcct ggcagccgca atcgagagcg gagttcgtca acggaatata tggaaagaat
    17761 tgttcggctc actcgtgctc ttcggaatta tcgccctcgg cagtttcttt atttttaatc
    17821 cttctgtttg gtacaatccg atcggcagaa tttcaatgat gatcggtttt catgaaaatt
    17881 accaagcgaa ggcgagtgat atttatccat ggtggcaacc gatagtttgg gtctcccgat
    17941 ccgtcgctca tcattcagat aagttttttt ccaaaagtcc tttaggaaaa agtcctgaac
    18001 acttcttttt ctccgctgac gaactgattt tcattttggc atgtatcgga tttttcaaga
    18061 ttccgagaga atatcgaatt tacttttatt ggtttatatt cgggcttttc ttccttttta
    18121 tttggggaac caaatgggtt caatatgctt gtgtcgttac tgcaccgcta tgcatcgcag
    18181 cttatttcgg gtcaaagaag gtttcggttt ggctgaaccg aattaatccg taatgatcga
    18241 atgcgagaga ataatgtgac gatttcgtat ttgaaaatga aagttattgg tttgtaaacg
    18301 tttacatgat aaaatgatcg atatattttt cattaccatt gacaattgag gtaaccttat
    18361 ggcaaatatc acaaaagaag acgctttgac ttatcatcaa ctgaacggag tccccggcaa
    18421 aatatcggtt gttccatcaa aaccgcttga gacatttatc gatttgagtc ttgcttatac
    18481 cccaggagtt gcttttccgg tgcttgaaat cgccgaaaaa ccggaaacgg tttatgacta
    18541 caccgataaa gggaatctcg tagctgtaat ttcaaacggt accgctattt tagggctggg
    18601 aaatcgcgga tccttagctt ccaaacctgt gatggaaggc aagggtgttc tgttcaagaa
    18661 attcgccgat atagatgttt ttgacatcga agtcgataca acggataaag aacggtttat
    18721 tgatacggta gcagccatcg gaaatacttt cgggggaatc aatctggaag atatcaaagc
    18781 accggaatgt tttgaaatcg aacaacgtct ccagcaaatg ctgagtattc cggttttcca
    18841 tgacgaccaa cacggcacgg ccatcattct gggcgctgct ttattgaatg cgttggaaat
    18901 cgtcaacaaa aagatcgaaa gggttaaaat cgtcataaac ggtgccgggg cagccggaat
    18961 ttcctgcgcc cgccatttaa tccgtttggg tgtcaaacgc gaaaatcttt tcttatgtga
    19021 ttcgacgggt ttggtctata aaggccggca agatcatatg aatccgcaaa aagaagaatt
    19081 tgccaacggc gatcatccgg cgaaacttga cgaagtgatt cgagatgccg atgtgttttg
    19141 cggcctttcc accgcggatg ttttaacggc tgaaatgatt aagaccatgg caaaagatcc
    19201 ctgtattttt gggatggcga atcccaatcc ggaaataaaa tatgagatag ctcgggaagc
    19261 cagaccggat gcgattatcg ccaccggtcg ttccgattat cccaatcaaa ttaataatgt
    19321 actgggtttc ccttatattt ttcggggtgc actggatgtc cgcgcttcca agatcaacga
    19381 agaaatgaaa ttagccgcct cctatgcgtt ggcagaactg gctcatcttc cggtgccgga
    19441 ttatgtcttg aaagcttatg agattccatc tttggagttc ggccgcgatt atattgttcc
    19501 caaggctttt gataaacggg cgaaatggaa ggtttcagcg gctattgccg aagcggcaat
    19561 gaaatccggt gtcgccggaa ttcaaattga tcttgaagct tatcaaaaga gactcggtaa
    19621 atgattttat tctctaaaaa ctgaacggaa ttctttccca aagaaaaatc gcatcctctg
    19681 acggatcatt ggatcgggga tgcgatgctt tttaaaagat tgcagttctc atcatgatcc
    19741 ccatccgata aaagacattt cgacaggttt ttttaggcta tcccgcaaat aagtttttaa
    19801 tcaggctgat gtttttcagt ataattgaga aatacggagt accatggacc aatctgatca
    19861 gacgaatcgg gaacaagaac agccgagaca aaaaaagcat tcccttgtcc ttgcgacagt
    19921 tgttattttt atcgctctcg cagtcatagc ctctttaatg atatatttca accgtgatca
    19981 ccctgcggga gtaatcaaaa cagcgaacta tcttgtagcg attattttct tcatcttgtt
    20041 tgtaatcagc atcggattga cttttataat cgttaaagtg attcagcggg tcgactattg
    20101 gctcggaaca ttgccgcagt tcaccaaaca agcgaattta cagatcgaat cattgagatc
    20161 aaaagtggca ggtatgacgg atgatatcac agagccgatc attacagcgg aagcaaagtt
    20221 cagagctctt catgaccttt ttaaaaccaa aaaacctcgg agttagatca ttatgagtga
    20281 taaaaattgg aaacgcaaca cgctgctgat aggtgccggc atcggtttac tgactggttt
    20341 gctttcggcg tatcttcgaa ttcgaaaagc ggaatcgaac aacgaaatca taacatttaa
    20401 agcaaaagat acggcaaaaa tcggcacggc tgtatttgat tttatgaaaa aattcctctg
    20461 agaatgccca tctcccctta tctacccatc gtagtagtcg ggacattaag aagagatttt
    20521 gtaattcctc cgggtggcaa gcctctcaat gatattccgg gtggccatct gttgcatacc
    20581 gccgtccatt tgcatcactg ggaaaatcga atcggcttag tttctcgcat cggacccgat
    20641 ttcccgcagg aatggttgga tcatttgacc gaattaggca tggatgtgca cgggattaaa
    20701 cggaccgctt taccgattga acatcgtttt tttatccgat atgacggaaa ttaccagaaa
    20761 tccacccttt atcctttggc tcagtatgca aacgaaggtt tgccctttcc gaaagagtta
    20821 ttaggttata acgttcaaac gaataaaatc gacagtatga acgctaaaac cgatgaaacc
    20881 atcatcgcca gagatattcc cgaaaattat ctggaatcca cttttattca tctttgtccg
    20941 atggattata tgacgcacaa cttgctgaca cagcactttg cgcagttcgg tcctaaacgg
    21001 ataactttaa gagccggaaa agggtatatg gtgccgcaat tttggcatgc gattccgtta
    21061 ttggtcaaca gcttgaccgc ttttattacc tcgatttccg gtctgaaaag cttgttttcc
    21121 gagacaagag aaacggatat ctggaaaatg gcaaaaatca tttccgaatg gggacccgag
    21181 tatgtcataa tcgagcaaga taatttctca aacttgctgt gggatagaaa tcatcaggca
    21241 cgatatcgat taggacctta tcaaaacaaa gttgttaatc tgatcggcat tcatgatgca
    21301 ttttgcggag gatttatcgg cgaactgaaa aaaacctatg atccggtcaa agcattgatc
    21361 gccggcaatg tcgcatcttc catcgctatg gaagccaatc atccttttta tgcattggat
    21421 acgatcccgg gattacatga agcacgggct gattatttat atagtaaact tgaattacta
    21481 taaaggtttt caaatttatt atcggtttct caattgaaac cggcagaatg atttttccat
    21541 agatccgaaa atgaaatctt tattagcttg atgaataggt ggatgaacac ttttgcataa
    21601 tttgcattaa aataaccaat accattggca gaaggagaac aaaatgaatc gtttagggaa
    21661 atttattacc ggagccgcga tcggcggatt aattggcatc ggggcgattt tgctttttgc
    21721 tcctgacaag agagatgaat ggtttgatga aattcaggaa aaaacccaag atatccttgt
    21781 cgaatttaat cgggcggcac aagaatataa agaagaaatg gaagaagact tgaaagcccg
    21841 taaaggtgct taatattatt ttccgcagat aaaataacgg atcatgatct gtttttgaaa
    21901 cgattcatgg tccgtttctt ttt
//
LOCUS       DAOSMM010000036        21911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2377_length_21911_cov_18.810029, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000036 DAOSMM010000000
VERSION     DAOSMM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2377_length_21911_cov_18.810029"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..427
                     /gene="trxA"
                     /locus_tag="PK898_06420"
     CDS             95..427
                     /gene="trxA"
                     /locus_tag="PK898_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU19705.1"
                     /translation="MINEPIHVTDDAFEKTVLKSEIPVVVDFWASWCGPCRMVLPLME
                     ELAKEYAGKLIVAEVNTDENSARAMDYNVTSIPTLLFIKNGEVKYRHTGTLSKAKLEE
                     YANNLLAA"
     gene            complement(495..1778)
                     /gene="murA"
                     /locus_tag="PK898_06425"
     CDS             complement(495..1778)
                     /gene="murA"
                     /locus_tag="PK898_06425"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU19706.1"
                     /translation="MDSFIIDGGIPLRGEVIPSGNKNAALPLICATLLTDEPVILHNV
                     PLIKDITAMEKLLESMGVTIEKLKPSTWKFQANHVNIADLDPDLCRNVRASILLAGPI
                     VSRFGSFILPPPGGDVIGRRRVDTHLLALNRLGATAYYDRQYHFEVKNRLKGTNILMD
                     EASVTATENAIMACSLAEGTSILRNAASEPHVQELCQLLNLMGAKINGIGSNVLTIEG
                     VDKLHGAEFTIGPDYLEVGSYIGAAVVTHGEILIRNAGIQYLDMVKLVYNRLGVEWEE
                     IGSDILVPENQRLTIEPDIGGAIPEISVMPWPAFPSDLMSIAIVIATQAQGSVLFHDW
                     MYPSRMYFTDKLVGMGAQIVLCDPHRCIVQGPTRLYPEKLESPDIRAGMALLLATLAV
                     PGKSVIRNISQIERGYERVDEKLRALGARIERVEA"
     gene            2018..3151
                     /locus_tag="PK898_06430"
     CDS             2018..3151
                     /locus_tag="PK898_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19707.1"
                     /translation="MVEIRKVDTADKKQTGQFVQFHYDLYKGDPNWVPPFRSDIYMMM
                     NRKKHPFYEHSDAEFFTAWKDGKMVGRIAGLNNNSYNNFHHLKTVNFFLYDSIDDQEV
                     CNALFDAVAEWGRGKGEDTLVGSKGFSLYDGYGILVEGYEYRQMMNMSSYNYPYYKTL
                     LENYGFEKMNDFVSMNFTLPDFNLPEKVYKAAEIAKKRGYLHVYTFKNKKEIMGSALK
                     IAQAYAKSFENNWEYFPLSEREFLSLVDNVIKVVDPKMVKFIYNDKEELVGFLLGFPD
                     LSAAMQRHNGNLNPALILDLLREMKKTKYLSLNGMGILEEYRKKGGNALLYTAILDLL
                     KEHPNIYGMEATQMAESAKEIQVEMLTMGLKPTKRHRVFSKSI"
     gene            3209..4423
                     /locus_tag="PK898_06435"
     CDS             3209..4423
                     /locus_tag="PK898_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19708.1"
                     /translation="MEIRPLKRDYGISSRFIVGILLLVLIAVGGNVYVVLHTDLGAGF
                     YSYWLSSRVLFAQGENPYGSDIFEQIIRRFPEDRYLSGFTLPLYAVIPVFLFSFIDNF
                     EIALIIWMTILEALLVGIGMKTISAFQIDRRTFTPLKIGTVMLLSYYSVVAVLDGDIG
                     ILAVFLLMMALDAIRSQEDEFAGILLAFATIKYSLTLLPIIWIFIWCLANRRTTVITW
                     FFMILAFLILLSVLFMTNWITEFIRSVIYYYKYLNPLYFSRLIENWQPELGGRIGWAI
                     SGFLILIMIIEWIVNARGNVRAFEWVTALTITIGFLSGIPNIGKNLYMLWIPLAYAMD
                     KINLRWTHNGKWISLVLVLSFLVIPWLNQRGQVLFWQNPIDVLNIIFPAVCLILLYWN
                     RWWTIQSVIEPF"
     gene            4611..5774
                     /locus_tag="PK898_06440"
     CDS             4611..5774
                     /locus_tag="PK898_06440"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU19709.1"
                     /translation="MTRIIGILTGGGDVPGLNPAMKAMVTIALDNGIKVYGIRRGWAG
                     LLEYDLNNPESQKNIYQLSYSDIRTIDRSGGTFLHTSRTNPQRVKMNAVPEFLKDSPL
                     GVKVDEDTMDYTAHVLKVLDHFGIQTLITIGGDDTLSFSVRLHKEGFPVIAIPKTMDN
                     DVFGTDYCIGFSTAITRSVEVITNMRTSLGSHERIGVIELFGRNSGETSLLAAYLSGA
                     DRAVIPEVHFNIDKLTDMLVEDRRRNPSNYSLTTISEGAIMEGGDVIETGEMDAYGHR
                     KLGGVGMVVAEAIKKRSNINIMYQQVGYMMRSGAPDSLDRMVATSYGNLAAQLLIRND
                     TGRMVALHGGKYTTVPVNMVLAGKKCVDVPSFYDIEEYRPHIKDFLGVPMFLS"
     gene            5791..6741
                     /locus_tag="PK898_06445"
     CDS             5791..6741
                     /locus_tag="PK898_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOU19710.1"
                     /translation="MIVTTKKLFEHAYGKYAIGAYNINNAEQIIGLFRGNLDSKAPFI
                     VQISKGAREYTSKIMLEGLIRSADQMFPEAIFAVHLDHGDEETCYDCINSGFYSSVMI
                     DASALSFEDNIAVTKRVVDAAHAKGIVVEAELGKLGGVEEHVSVNDADAKLTDPDSVK
                     EFVDRTHVDSLAVAIGTSHGAFKFSGTQSLHFDVLAEIQKRVPGFPLVLHGSSSVPQE
                     EIDRINAAGGNIKGAKGVDPSQFKRAAQLGVTKINIDTDGRLVWMRVHREYFRDHPEN
                     FDFRPIGKIFMAEYAKFIADKNEKLGSAGQLDSVREFLSK"
     gene            6884..7663
                     /gene="surE"
                     /locus_tag="PK898_06450"
     CDS             6884..7663
                     /gene="surE"
                     /locus_tag="PK898_06450"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU19711.1"
                     /translation="MQKPLEIVVTNDDGIFAPGILALASTAMKIGNVSVIAPDKNWSA
                     SGHQKALGRPLRVDPVSFPLDIPAYATDGGPSDCVALAGLGFLDKKIDLVLSGINPTS
                     NVSRDITYSGTVTSALESTIWNMKGIAFSLDAGSIKIKEIDFSETEPMILTVVRTFLQ
                     HELPPFTILNVNLPYKVKKPNPDYVITREGSRYYRDELIRQVDPFGKPYYWFGGDPPY
                     GDEEEGTDCGELARGNISITPLQLDLTAEQLIPSLQQWEWK"
     gene            7673..8179
                     /locus_tag="PK898_06455"
     CDS             7673..8179
                     /locus_tag="PK898_06455"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19712.1"
                     /translation="MIQKRCILLFSSLLLFVLSGCASNPTAIPAPVYTAPLPTRIVAT
                     KVIIQNEPDCIDNFYYIDDVNYPDGTYVEPGQKFLKEWEVMNNGSCNWIDGYRLRFVS
                     GEQMGAPDSVPIPHVEVGKKGKFSVEFTAPKEPGTYRSIWKPFNNRNRSFGETVYLEI
                     VVNGYPAE"
     gene            complement(8304..10811)
                     /locus_tag="PK898_06460"
     CDS             complement(8304..10811)
                     /locus_tag="PK898_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOU19713.1"
                     /translation="MAGIDRFTQRARRVLSLAHQEADKNNETIIGTEHLLIGLLLVEG
                     STAGKVLQDLDVSADRVREALSKMEPKTEILGIPEAGQEEALSAEVQDILKRAVIQAS
                     KNGQVYVSTEHLLLSLIETENSRAVAVLKRLSISPEQIRKQLKRVLEEGNSAPASNST
                     EEVKPKPVKKQRTDKTTSNSPLIDQLATDLTAKAAAGKLDPVVGRETEIARVIQVLAR
                     RTKNNPALVGEPGVGKTAIVEGLAQAIVEGNVPAQLLNKRVLQLDVGSMVAGTMYRGQ
                     FEDRMKRVIEELKNTKAILFIDEIHMLVGAGSSGSAVDAANILKPALSRGEVQVVGAT
                     TSDEYRKNIESDAALARRFQQILVNEPTPEETIQILKGLRPRYEEHHHLNISDEALEA
                     AVKLSVRYVSERFLPDKAIDLIDEASSRVRMYKNPSTALTDDYMAELRKTRKDGLLAI
                     EQGDADLAASLYERGLVLEDRIEEASKIWNREEAPVVTAEDIAEIVSMWTGIPVMQMA
                     EEESERLLRMEEELGKMIIGQNEAISEISHAIRRSRSGLKDSNRPIGSFLFLGPTGVG
                     KTELTKALAKYLFGSEEAMIQLDMSEFMERHTVSRLTGAPPGYVGYEEAGQLTEAIRR
                     HPYSIVVFDEIDKANPEVLNMLLQIMEEGHLSDARGKKVDFRNTIIVMTSNIGADVIR
                     RQNNFGFNLIEGKDEEEQESYEEMRKKLMDQLKKSFRPEFLNRLDNIVVFRMLNLEDM
                     QQITEIEIGKVNQRLADKQITLTVSESALEKLTRDGFDPEMGARPLRRVIQQQIEDPL
                     SDALLAREFSNGDQVLITLDDKNEIAFRKVEPQREEL"
     gene            complement(10981..12000)
                     /locus_tag="PK898_06465"
     CDS             complement(10981..12000)
                     /locus_tag="PK898_06465"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOU19714.1"
                     /translation="MPTHEPLILEYRNEVIDNVHMGYVCIVDENSKVLFHIGDPSEYV
                     YYRSASKPIQALPVIMRDLDKKYGITEEESVIFSASHSGEPFHIAILESIFAKAGLKE
                     EMLIMKPTYPGYFPAKLELLKKDLPPRKFYHNCAGKHAGAMLLQRELGGKVEDYWRPD
                     SPAQKEIKRAVMALSETNEDNTKVGIDGCGVPVFAVGLKNIAAGYKNLACIDTIRDEK
                     LREAAGVYLPRINRYPHIIRGTGYLCSLMNRDPNLISKDGAAGVYGCGMRKERLGISF
                     KMSSGEDTVWPLILKSIFTQIGGQDPQTLEMLDKLRPDSVFNDNDLVVGHYESLICLD
                     RKPIR"
     gene            complement(12070..13533)
                     /locus_tag="PK898_06470"
     CDS             complement(12070..13533)
                     /locus_tag="PK898_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19715.1"
                     /translation="MEQNKKITWFEGIVNPEIEEANPLLSWIWCGFLFLLGMFLWGYF
                     LNFGKIPFDFHDWAEVNAPRIAFLKDAVTKGVLPLHMQDASALRGVTDRYMALPDVLL
                     SPQILLLKWLDVGDFILIHTWLLYAIGFWGLYRLKKSMHLSLFGFTWFFLLFYFNGHI
                     TSHYAVGHITWGGYFLFPWFVYLVIRMINGDHSWKWVAKISFLLFFMFLQGSFHQFIW
                     CLLFLAFLALTGRKNLFTVLKAGMFSCLLSMVRIIPPALQASAFDDDFLGGFTSPIQL
                     IRSFIQIIPPSESLNSAVTGGNLGWWEFDHYIGVSGALFLLIFGILWLKNRRKETGYP
                     AILVPMIVLTLFSIGKFYGIFRLLPIPLFSGERVSARFLILPLVFLMAEAVYAMQQSM
                     EIRKFDVLSVSIPLLILLFGLPEFWSHLETWKVLNAFQAFPYTYTDLTIKTVANHPDP
                     SYTNGLLIGMVITILTAILLAGLVWRENRHTKNILVP"
     gene            complement(13662..14555)
                     /locus_tag="PK898_06475"
     CDS             complement(13662..14555)
                     /locus_tag="PK898_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU19716.1"
                     /translation="MNSAEKSALQKKSNLILLLTAIIWGSGFIPQKICANSMTAFAFN
                     GFRYLIGSLSVLIITHFRLPENRRAWRITFLSGTILFLAADLQQFGIRYTTVGNTGFI
                     TTIYVVLVPFMGWLLFRQKITRSAFWAAAIALIGMYLLSTSGKALSTISAGDGIVFIG
                     SVFWAIHILVVGKAMGRVDAMRFSAGQFLVCAFWNLLCWLIFDRGNLSGVRESIPLLL
                     YTGIVVIGGGFTLQAVGQKHADESQAAIILGLEAVFASFFGILFFRESFSFIQAAGAA
                     LIFISVVISVKGTPNLELPPI"
     gene            complement(14557..15315)
                     /locus_tag="PK898_06480"
     CDS             complement(14557..15315)
                     /locus_tag="PK898_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU19717.1"
                     /translation="MISEWNKSIWLTRSAFMPGQNWLVNGAILKGEKMALVWETLAYP
                     ADMQAMSFLLAKFDFVVAAYSHADWDHCLGTNGFSFSNVIAQQNAIRRFKFELPATLK
                     KLNAKYSKAWEDVCPVPPDFVFSDKLNLDFGSFRLELSSFPGHTPDGMVGYIPEYKML
                     LAGDSVEEIPVINDPKEVPSWIKHLKKWLTEPDIQSVLPGHGEITDLSLLEKNLKYLI
                     ALTEKSPLDVETDNPFYLDVHQENCKLMGCSVGK"
     gene            15414..15851
                     /locus_tag="PK898_06485"
     CDS             15414..15851
                     /locus_tag="PK898_06485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOU19718.1"
                     /translation="MAIVILHASIRDLESVLYLEKVCFEEDAWTILDLTYALSAPNKI
                     RFKALVDENFAGFAAAENHTKECISWITTIAVLPEYRNRGVASALLDACEADLSYPKV
                     RLAVNVHNIAAIRLYRKYGYKEIETWKNYYMNGDNALIMEKQL"
     gene            15848..18196
                     /locus_tag="PK898_06490"
     CDS             15848..18196
                     /locus_tag="PK898_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOU19719.1"
                     /translation="MKVEKKRLAAEIFLSLLILLGIFYSFYNMNWDSGALLHPDEYGM
                     ANTISQLSIPSSIGNYFNTRESTISPYNKYDLMGNKVSDGPDNRMRWGQLPITLIRLT
                     AEAFGQTGYLQLRKTGRYLSAIMNLGTLFLLFLITKRLLNDRLIALLATALSSLAVMQ
                     IQQSHFMTSDNFAVFFTTLTLYAAVRISQEEILLRDPQSGKYRVTRDGWLWFALFGVF
                     LGMAVSSKINQAVTGGMLLFAVFINIADLKLTSKKDFNQIFKLAFGMCVFSAVCALLT
                     FRVLQPMSFRSPHGDTGFFTWHFNKDWIDSMLVSMAESGGNGGGPPAEQWAHRTPVLF
                     PLINMILYGMGTPLGLAVWISALAAVWKMFRSRGRLWKILLIPTAWSFLYFLFMGTRF
                     VKSIRYMLPVYPTLCLLAAWGIASLWRKYNGRKKILPGVISVLVVGGACLWASLFVKT
                     IYMQPHSRVEAVTWIYDHIPAMIQLEGKSDSEGTIVTIVPSVPSTLAITASVPFETDI
                     RVQQNTVIDRLLIPHAANYTFSDSTLRIAIVHSDGTILSQTETSIQAGTKDQAINLSL
                     PAAVLLPAENYRLLLSIPQGNGITLRRNILANENWDEGLPFPLYGKDPFGQLYTGITN
                     EIRWPDSEDKKEMLLDVLDQADYIILPSQRGIWSSVRIPLTYPMTIDYYESLFDGSLG
                     FELAAQFQRPFKLGPLYFSDLTGKFYWNQAPVLPVKNLSPFAAEEAFSVYDHPPVWIF
                     RKTADFDLSKAKAILDRTDLSKVIVQGPTEAVWPEGYADSEE"
     gene            18305..18379
                     /locus_tag="PK898_06495"
     tRNA            18305..18379
                     /locus_tag="PK898_06495"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18339..18341,aa:Val,seq:cac)
     gene            complement(18443..18793)
                     /locus_tag="PK898_06500"
     CDS             complement(18443..18793)
                     /locus_tag="PK898_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOU19720.1"
                     /translation="MSIPASETVILITRNGMGEGTPELQLKLISVYLKLIDENNILPA
                     AICLYTDGVKLATNGSPVIDTLKSLEKKGVRLILCSTCLNFYNLVDQVAVGIVGGMSD
                     IIDAQFKAEKVISI"
     gene            complement(18995..19888)
                     /locus_tag="PK898_06505"
     CDS             complement(18995..19888)
                     /locus_tag="PK898_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU19721.1"
                     /translation="MSENNSPIFCNYDESDYQNRFWGEGKRAYEDASEAIALERLLPK
                     SGRLMLEIGAGAGRNTLRYRGYERIVLMDYALTQLQQAQQRLGKSDRFIYVTADVYHM
                     PFADGLFDGATMIRVLHHLSEINSALAEIQRVMQYQSVFILEFANKRNLKAIFRYLTG
                     KQDWNPFSPEQVEFAELNFDNHPDTVRKVLTENGFIPEKVLTVSNLRIESLKKEKNLG
                     WMLKLESALQYLASPFKLSPSIFIRNRQSSEKPDAVEWEFFKCPNCHSYEIVEESEKV
                     HCHSCGRDYPIIDGIYDFRLK"
     gene            complement(19919..20018)
                     /gene="ffs"
                     /locus_tag="PK898_06510"
     ncRNA           complement(19919..20018)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK898_06510"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(20105..20728)
                     /locus_tag="PK898_06515"
     CDS             complement(20105..20728)
                     /locus_tag="PK898_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOU19722.1"
                     /translation="MASKSNDFYFNAFVEQSEFSIQIVEKVKNIFENYNTINLRTSMD
                     EVHLLEHTADIKHHEIMEKLSKEFIPPIEREDIVVITQLLDDLTDYLEDIPILLYTYH
                     VKTIDQAMMQFMDVIDQSVHQVMKLLSVFSDFKKDLTIHKYIIDINRLEENGDILFHE
                     FMYKVFEEEHDARYLIGQKEMFTKFEGILDTAEDIANEVGNIIMKNS"
     gene            complement(20750..21802)
                     /locus_tag="PK898_06520"
     CDS             complement(20750..21802)
                     /locus_tag="PK898_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOU19723.1"
                     /translation="MSISFSEFIYLISHNIPLLITVILTLSVIIVNGWTDAPNAIATC
                     IGTRSLKPQYALIMAAVFNFLGVFVMTILNSTVAQTVYKMVDFGTNSSEATLALCAGM
                     VAIVLWATVASFMGIPTSESHALIAGLSGAAIGLHGSFAGINGSEWIKVIYGLVLSTV
                     MGFGFGFLAYKLVKLIFQSIDRRRTQKFFRHAQVFASGAMAFMHGAQDGQKFIAVFML
                     GIALSQGETGQSVFVVPLWLMILCSLVMSLGTSIGGYSIIKSVGMNMVKLEPFQGFAS
                     DIAAASCLLISSLFSLPVSTTHTKTTAIMGVGAGKRISSVNWGIVKDMVWAWVLTFPG
                     CGLIGFLVVKLFIYLF"
BASE COUNT         6121 a         5069 c         4621 g         6100 t
ORIGIN      
        1 ataacccaat aaaaaaatac ggtaaaattt gaaagtgagt tcattttaag ctgaatttga
       61 ttcagcgatt tggaaggcag aaaggtaaaa tatcatgatc aatgaaccga ttcacgttac
      121 ggatgacgct tttgaaaaaa cagtccttaa atcagaaatc cccgttgtgg ttgatttttg
      181 ggcgtcttgg tgcggacctt gcagaatggt tctccctttg atggaagaac ttgcaaagga
      241 atatgccggt aaattaatcg ttgcggaagt caatacggat gaaaacagcg cccgggcgat
      301 ggactataac gtcacatcta ttccgaccct cttattcatt aaaaacggtg aagtaaaata
      361 tcgccatacc ggaactttat ccaaggcgaa attggaagaa tacgccaata atttattagc
      421 cgcataattt caacacgaaa tgaaaaggag ctgtttcgaa gcagctcctt ttttttatct
      481 ttcaaacaac tttttcaggc ttcgacccgc tcgatgcgtg ctcctaaagc ccgcagtttt
      541 tcatccactc gctcatatcc gcgttcgatt tgagaaatat tgcggataac ggattttccc
      601 ggaacagcca acgtcgccag caacaacgcc atccccgcac ggatatccgg cgattccaat
      661 ttttcggggt acaaacgggt aggtccctga acgatacaac gatgcggatc gcagagaaca
      721 atttgagcgc ccataccgac caatttatcg gtaaaataca tacggctcgg atacatccaa
      781 tcatgaaaga ggacgcttcc ctgcgcttgt gttgcgatta cgatcgcaat gctcattaag
      841 tcggaaggaa atgccggcca gggcataacg ctgatttccg gaatcgctcc gccgatgtcc
      901 ggctcaatag tcaatcgttg gttctcgggt accagaatat cactgccgat ttcttcccac
      961 tcgacaccca atcggttata aaccagcttt accatatcca gatattgaat ccccgcattc
     1021 cggatcaata tttcaccgtg agtgacaaca gccgccccga tgtaactgcc gacctccaaa
     1081 taatccggcc cgatggtaaa ttcagctccg tgtaatttgt caactccttc aatcgtcaaa
     1141 acgtttgaac cgattccgtt gattttggca cccattagat ttaacaattg gcacagctct
     1201 tgaacatgcg gttcggatgc cgcattccgt aaaatcgaag tcccttcagc gagagaacaa
     1261 gccataatcg cattttcggt tgcggtgaca ctggcttcat ccatcaaaat atttgtcccc
     1321 ttcaacctgt ttttcacttc aaagtgatat tgcctgtcgt aatatgccgt cgcacccaat
     1381 cggttcaaag ccaacaaatg tgtatccact ctgcggcggc cgattacatc acctccgggc
     1441 ggcggaagaa taaaactgcc gaagcgactg acaatcggac cggctaacag gatggaagct
     1501 ctgacattcc gacaaaggtc ggggtccaaa tccgcgatat tgacgtgatt ggcttggaat
     1561 ttccaagtgg agggtttcag tttttcaatg gtaaccccca tgctttcaag taatttttcc
     1621 attgcggtga tatctttaat taacggcaca ttatgcaaga tgaccggttc atcggttaat
     1681 aaagtcgcac aaatcaaagg taaagccgcg tttttatttc cggatggaat aacttcaccc
     1741 cgcaagggga ttccgccatc aataataaat gaatccatac atcgtccttc taatcaattc
     1801 gttgtgtcta ttatagctta atcgatttca tcttttttga aaaaaagttt ctattttctt
     1861 tcccttaaat ctgtcatcga atcctatttt ctgtaaaatt ttagtcagcc gtcatattta
     1921 accttccggt taaggtaaaa tagtgtaaat agccaaaatt atcctctact attattatca
     1981 tcattttatt ctttgaggtt aaaggagcct tcatgccatg gtagaaatac gaaaggtcga
     2041 tactgcggat aaaaaacaaa caggccaatt tgttcaattc cattatgatc tatacaaagg
     2101 agatccgaat tgggttcctc ctttccgatc cgatatttac atgatgatga atcgtaaaaa
     2161 gcatcctttc tatgaacatt cggacgctga attttttacc gcatggaaag acggaaaaat
     2221 ggtgggacga attgccggat taaataacaa cagctacaac aattttcatc atttgaaaac
     2281 cgtcaacttc ttcttatatg attccatcga tgatcaggaa gtttgtaacg ccctttttga
     2341 tgccgtggca gaatggggaa ggggaaaagg ggaggacacc ttagtcggtt cgaaaggatt
     2401 ttccctgtat gacggttatg gaatattagt tgaaggctat gaatatcggc agatgatgaa
     2461 tatgtcatct tataactatc cttactataa aactttattg gaaaattatg gctttgaaaa
     2521 aatgaatgat tttgtttcca tgaattttac gcttccggat ttcaacctgc ctgaaaaagt
     2581 atataaagcg gctgaaattg ccaaaaagcg cggttatctt catgtctaca cgttcaaaaa
     2641 taagaaagaa atcatgggaa gtgctttgaa aatcgcccag gcttatgcca aatctttcga
     2701 aaataactgg gaatattttc cgctttcaga gagagaattc ctcagtttgg tggataatgt
     2761 catcaaagtc gtcgatccga aaatggtaaa gtttatttat aacgataaag aagaactggt
     2821 tggctttctg ctgggatttc cggatttatc cgccgcaatg caacggcaca acggcaatct
     2881 gaatccggca ctgattctcg atctgttacg agaaatgaag aagacgaagt atttgtcgtt
     2941 gaacgggatg ggaattctcg aagaataccg aaagaagggg gggaatgccc tcctttatac
     3001 ggcaatcttg gatcttttaa aggaacatcc caacatttac ggcatggaag cgactcaaat
     3061 ggctgagagt gccaaagaaa ttcaggtaga gatgcttacc atgggattaa aaccgacaaa
     3121 acggcatcga gttttttcaa aatcgatcta atgaggctcg tcatctgaaa aaacttcctg
     3181 ccggcttttt aaaaaagaga aggaatcaat ggaaatccgt cccttaaaac gcgattacgg
     3241 tatttcatcg agatttattg tcggaatcct tcttttagtt ttgatagccg tcggcggaaa
     3301 tgtttatgtt gtgctccata ccgacttggg agccgggttt tacagttact ggctttcgag
     3361 tcgggttttg tttgctcagg gggagaaccc ctacgggtcc gatatttttg aacagattat
     3421 ccggcgtttt ccggaagacc ggtatcttag cggtttcact ttacctctct atgccgtcat
     3481 cccggttttt ttgttttctt ttatcgataa tttcgaaatc gcattgatta tttggatgac
     3541 gattttggaa gcgttgttag tcggcatcgg aatgaaaaca atctcggctt ttcaaatcga
     3601 tcgccgtacc tttacgccgt tgaaaatcgg aactgtaatg ctgctgagct attattcggt
     3661 cgtagctgtt ttggacggcg atatcgggat attggcagtc tttcttttaa tgatggcatt
     3721 ggacgccatc cgctcacaag aggatgagtt tgccggaatc ttactggctt tcgcaaccat
     3781 taaatacagt ttgacgcttt taccgatcat ttggattttt atttggtgcc ttgcaaaccg
     3841 ccgcacaacc gtcatcacct ggtttttcat gattctggca tttctcattc tgttgtccgt
     3901 tctttttatg accaattgga ttaccgaatt catccgttcg gtcatctatt attacaaata
     3961 cttaaatccg ctttatttca gccgattgat tgaaaactgg caacccgaat tagggggaag
     4021 aatcggctgg gcaatttcgg gttttcttat tttaataatg atcattgaat ggattgtgaa
     4081 tgcacgcggc aatgttcgcg cctttgaatg ggtgacagcc ctgacaatta ccatcggatt
     4141 tctttccggt atcccgaata tcggcaaaaa cctttacatg ctatggattc cgttagccta
     4201 tgctatggat aaaataaacc tgcgctggac ccataacggc aagtggatca gtctggttct
     4261 tgtcttgtct tttttggtga tcccatggct taatcaacgg ggacaggtac tgttctggca
     4321 aaatccgatt gacgtgctga atatcatttt cccggccgtt tgtttgattc tgctttattg
     4381 gaatcgttgg tggactatac aaagcgtcat cgaacctttt tgagaccttt tttaaaattc
     4441 tttcgatcac taatatatta tttttcataa atattcgtca tacaaacaat ggcaaagggt
     4501 ctgctatagt ataattttcg ctgaatttag cgctttaatg cttcaaaatc aggtgaatga
     4561 aaagccgaac agcattttca tcttaacctt aatcacaagg agttaaacac atgacgagaa
     4621 ttataggtat cctgaccggt ggaggtgatg ttccgggttt gaatccggct atgaaagcaa
     4681 tggtaaccat tgctttggat aacggaatta aagtttacgg tatccgccgt ggatgggccg
     4741 gtttgttgga atatgacctg aataatccgg aatcgcagaa aaatatttat caactgtctt
     4801 attcggacat ccgcacaatt gatcgttccg gcggcacatt tctgcacaca tccagaacga
     4861 atccccaaag agtcaaaatg aacgccgtac cggagtttct gaaagattct ccgttagggg
     4921 taaaagtcga tgaagataca atggattaca ccgctcatgt attaaaggtg ttggatcatt
     4981 tcggtatcca aacgctcatt acgatcggtg gggatgatac cctgagcttc tcggttcggc
     5041 tccacaaaga agggttcccg gtcattgcga ttcccaagac gatggataat gatgttttcg
     5101 ggacggatta ctgcatcgga ttctcaaccg ccatcacccg ttccgtcgaa gtcattacca
     5161 atatgcgaac ctctctcggc tctcatgaac gaatcggcgt gatcgaactt ttcggtcgga
     5221 attcgggtga gaccagcttg ctggccgcat atctgtcggg agccgaccgc gctgtcattc
     5281 cggaagtgca ttttaatatt gacaaattaa cggacatgct cgtcgaagac cggcggcgca
     5341 atccgtcaaa ctattcgctg acaaccattt ccgaaggagc gatcatggaa ggcggcgatg
     5401 tgatcgagac gggtgagatg gatgcttacg ggcaccgcaa gttgggcggc gtcggaatgg
     5461 tcgtcgcaga agcgattaag aagcgatcga atatcaatat tatgtaccaa caggtaggat
     5521 atatgatgcg ttccggcgca ccggattcgt tggatcgaat ggtcgcaacc agttacggga
     5581 accttgccgc gcaattgttg atccgaaatg ataccggcag aatggttgct ttgcacggag
     5641 gaaaatatac gaccgtcccg gtcaatatgg ttctggctgg gaaaaaatgt gttgacgtcc
     5701 cgtcttttta tgatatcgaa gaataccggc cgcacattaa ggatttcctt ggtgtgccta
     5761 tgtttttaag ctgattctga tggaggaata atgatagtaa caacgaaaaa attatttgaa
     5821 catgcttacg gcaaatatgc catcggagca tataacatca ataacgccga acaaattatc
     5881 ggcttgttca ggggtaacct tgacagtaaa gctcctttta ttgtgcaaat ttcgaaaggc
     5941 gcacgggaat ataccagcaa gattatgctg gaaggcttaa ttcgcagcgc cgatcaaatg
     6001 ttcccggaag caatttttgc ggttcatttg gatcatggcg atgaagaaac ctgctatgac
     6061 tgcattaata gcggctttta cagttccgta atgatcgatg cctctgcatt aagctttgaa
     6121 gataatatcg cggtcaccaa acgagtagtc gatgccgcac atgccaaagg gatcgtagtg
     6181 gaagccgagc tcggcaaatt gggcggtgtc gaagagcacg tttccgttaa tgatgcagac
     6241 gccaaactga ccgatccgga ttcggtaaaa gagttcgtcg acagaaccca tgtggattcg
     6301 ctggcagttg cgatcggaac cagccacggt gcattcaaat tctccggaac ccaatctctg
     6361 cattttgacg ttttggcgga gattcaaaag cgggtccccg gtttcccgtt ggttttgcac
     6421 ggttccagtt cggttccgca ggaagaaatc gatcgaatca atgcggccgg cggcaatatt
     6481 aaaggcgcaa agggcgttga tccgagccaa ttcaaacgcg ctgcccagtt gggcgtgaca
     6541 aagatcaata tcgataccga cggacgtctg gtttggatgc gtgtccatcg tgaatatttc
     6601 cgcgatcatc cggagaattt cgatttccgg ccgatcggta aaattttcat ggctgaatat
     6661 gcaaaattca tcgccgataa aaatgagaaa ctcggatctg ccggacaatt agacagcgtt
     6721 cgggaatttc tctcaaaata aacagactct gactcaaaaa acggcgtgct tcggggaaac
     6781 ccggcacgct gttttttttc aaacaccgat tcattttcaa gccaaaaagc ttcgagctgt
     6841 tggtagaata gtaatagatt cttttagatc aattgaggat ttaatgcaaa aacctttgga
     6901 aattgttgta acgaatgatg acggaatttt tgcgccgggg attctggcct tggcttcaac
     6961 cgctatgaaa atcggaaatg tttccgtcat agcccccgat aagaactggt ctgcttccgg
     7021 gcatcaaaaa gcactggggc gcccgctgag ggtagacccg gtttcttttc ctctggacat
     7081 tccggcttat gccaccgacg ggggtccgtc ggattgtgtc gcactggcgg ggttgggctt
     7141 tttggataag aagattgatt tggtgctctc cggaattaac cccacttcga acgtgagccg
     7201 ggatattaca tattccggaa ctgtcaccag cgcattggaa tcaacaatct ggaatatgaa
     7261 aggaatcgct ttttcgctgg atgccggcag cattaaaata aaggagatcg atttttccga
     7321 gacggaaccg atgatcttaa cggttgttcg aactttttta caacatgaat taccgccttt
     7381 tacgattttg aatgtcaatc tcccttataa agtaaaaaag ccgaatccgg actatgtcat
     7441 cacgcgggaa ggctcccgat attatcggga tgaattgatc cgccaagtcg atccttttgg
     7501 aaaaccgtat tattggtttg gaggagatcc cccgtacggg gacgaagagg aaggaacgga
     7561 ttgcggcgag cttgcccgcg ggaatatcag tatcactccc ctgcaactgg atttgaccgc
     7621 cgaacagcta attccgtctc ttcagcaatg ggaatggaaa taaatcggcg gcatgataca
     7681 gaaacgctgc attcttcttt tttcttcttt attattgttt gtactttccg gatgcgcaag
     7741 caatcctacg gcaatccccg cacccgtata cacagctcct ttgccaactc ggatcgttgc
     7801 caccaaagtt attatccaaa atgaaccgga ttgcatcgat aatttttact acattgatga
     7861 tgtcaattat ccggacggca cttatgtgga acccgggcaa aaatttctga aagaatggga
     7921 agtgatgaat aacgggagct gtaactggat tgacggctat cggttacggt ttgtttcggg
     7981 tgaacagatg ggtgcgcccg attccgttcc gattccgcat gtggaagtcg ggaaaaaagg
     8041 gaaattttcg gtagaattta ccgctccgaa agaacccggg acatatcgat caatttggaa
     8101 accgtttaac aatcggaatc gttccttcgg agaaacggtt tatttggaaa tcgttgtcaa
     8161 cggatatccg gcagaataaa ttcgaaaatc gatattacat aaaaaaaacc gcaccccgtg
     8221 ccgttgtctt ttatctcggt tgggggtgcg gtgcatttta gcagccatct gcaaaatttt
     8281 atctcatttg cagcgttttc agattacaat tcttctcttt gcggttcaac cttccgaaaa
     8341 gcaatttcgt ttttgtcatc caaagtaatc aatacttggt ctccgtttga aaattctctt
     8401 gccaataagg catcggataa cggatcttcg atttgttgct gaatcactct gcgcagcgga
     8461 cgggcaccca tttcgggatc gaaaccatcc cgggttaatt tttccaaggc gctttcgctg
     8521 acagtcaaag taatctgttt atcggctaat ctttggttca ctttgccgat ttcgatctcc
     8581 gtaatctgct gcatatcttc caaattcaac attcggaaga caacaatgtt gtccagacgg
     8641 ttgaggaatt cggggcggaa agactttttc agctgatcca tcagcttttt ccgcatctct
     8701 tcatagcttt cctgctcttc ctcatctttt ccttcgatca gattgaagcc gaaattattc
     8761 tggcgccgga tgacatccgc accgatgttg gatgtcatga caatgatcgt attccggaaa
     8821 tcgaccttct tcccgcgagc atccgaaagg tgtccctctt ccatgatttg caacagcata
     8881 ttcaacactt ccggattcgc tttgtcgatt tcgtcaaaaa ccacaatgga atatggatgt
     8941 ctgcgaatag cttccgtaag ttgtccggct tcttcataac cgacatatcc cggaggcgct
     9001 cctgtcaacc ggctaacggt atgcctttcc ataaattcgg acatatccaa ttggatcatc
     9061 gcttcttcgc tgccgaaaag atattttgcc agtgcttttg tcagttccgt tttgccgaca
     9121 cccgtcggtc ccaggaacaa gaacgaaccg atcggtcgat ttgaatcctt cagaccggag
     9181 cgtgatcgtc gtatcgcatg tgaaatttcc gatatcgctt cgttttgccc gataatcatc
     9241 ttaccaagct cttcttccat acgcagcaag cgctcggatt cttcttccgc catttgcata
     9301 accggaatcc ccgtccacat ggaaacaatt tccgcgatat cttcggcggt tacgaccggt
     9361 gcctcttccc gattccagat cttactggct tcttcgatcc gatcttccaa tacaagacct
     9421 ctttcataga gcgacgctgc caaatcggca tcgccttgtt cgatcgccag caatccgtcc
     9481 tttctggttt tgcgcagctc agccatataa tcgtcggtga gtgccgtgga aggattttta
     9541 tacatgcgga ctcttgaaga agcttcatca atcaaatcaa ttgctttatc cggtaaaaag
     9601 cgttcggaaa cataacgtac ggataatttg accgccgctt ccaaagcttc gtccgaaata
     9661 ttcaaatgat gatgctcctc ataccgcggg cgtaatcctt tcaagatttg aatcgtttct
     9721 tcgggtgtcg gttcattgac caggatttgt tggaaacgcc tcgccaacgc cgcatcactt
     9781 tcgatgtttt tccgatattc gtcggatgtt gtcgcaccga ccacctgaac ttctccgcgg
     9841 gataacgccg gtttcaatat attcgcagcg tcgaccgcag aaccggaaga tccggctccg
     9901 accaacatat gaatttcatc gataaacaat atcgctttgg tattttttaa ttcttcaatt
     9961 acccgcttca ttcgatcttc aaattgcccg cgatacattg tgccggcaac catagaaccg
    10021 acatccaact gcaatacccg cttgttcagt aattgcgccg gaacgtttcc ttccacgatc
    10081 gcctgggcta aaccctccac aatcgcggtt ttaccgacac ccggttctcc gaccaatgcc
    10141 gggttatttt tcgttcgcct ggcaaggact tggataaccc gagcaatttc ggtttctctg
    10201 ccgacaaccg gatccagttt ccccgccgcg gcttttgcgg ttaaatcggt tgccaactga
    10261 tcgattaacg gtgaatttga agtcgtttta tccgtccttt gtttctttac cggtttcggt
    10321 tttacttcct ctgtcgaatt ggatgccggt gcagaattcc cttcttccaa cacccttttc
    10381 agctgcttac gaatctgttc gggactgatg ctgagccttt ttaacaccgc aaccgcccgt
    10441 gaattttcgg tctcaattaa actgagtaat aaatgctccg tactgacata aacctgtcca
    10501 tttttagagg cttgaattac cgcacgcttt aaaatatcct gaacttcggc ggataatgcc
    10561 tcttcctgtc ccgcttcagg aattccaagt atttccgtct tcggctccat cttggataag
    10621 gcttcccgta cccgatctgc gcttacatcc aaatcctgca aaactttccc ggcggtagag
    10681 ccttcaacca ataagagccc tatcagaaga tgttccgttc cgattatcgt ttcgttattt
    10741 ttatcagctt cttgatgagc aagacttaat acccgcctcg ctctttgtgt aaatcgatct
    10801 attcctgcca ttttttatct cattttctta ctataaccga tataactcat cagttcttcg
    10861 ttattaattt taacagaata attttcaaaa ctatcctgcc gaattgtacc agagttcata
    10921 tctaatatac gtttgaaagg gcagtttcgt ttgttagaaa tctattaatt tctccgaaat
    10981 ttatcgaata ggcttccgat cgagacaaat cagggattca taatgtccca cgaccaaatc
    11041 attatcattg aacacactat ccgggcgcag tttatccagc atttccaatg tttggggatc
    11101 ctgtccgccg atttgagtga agatgctttt taatatcaac ggccaaaccg tatcttcccc
    11161 gctgctcatt ttgaatgata tccccagccg ctctttccgc ataccgcatc cgtaaacgcc
    11221 tgcggctcca tcctttgaaa taagattggg atcccgattc atcaggctgc aaagatagcc
    11281 cgtcccgcga attatatgtg ggtaacgatt gatacgcggc agatatacgc cagccgcttc
    11341 ccgcaatttt tcatcccgaa tcgtatcgat acaagctaaa tttttatagc cggcagcgat
    11401 attcttaagt ccgaccgcaa agactggcac accgcaaccg tcaatgccga cctttgtatt
    11461 gtcttcattc gtttcactca gcgccataac cgctcttttt atttcctttt gagcgggaga
    11521 atccggccgc caataatcct ccacctttcc ccctaattct cgctgcagaa gcattgctcc
    11581 ggcatgcttt cccgcacaat tatgataaaa cttgcgcggc ggtaaatctt tcttcaataa
    11641 ttccaacttc gccggaaaat aacccgggta agttggcttc attatcaaca tttcttcctt
    11701 cagtccggct ttggcaaaaa tcgattccaa tatggcgata tggaaaggct cgccggaatg
    11761 cgatgccgaa aaaattacac tttcttcctc tgtaataccg tattttttat ctaaatcacg
    11821 catgatcacc ggtaaagctt ggatcggctt ggatgccgag cggtaataca catattcgga
    11881 cggatcaccg atatgaaaca aaacttttga attttcatca acgatacaga catatcccat
    11941 atggacatta tcgataactt catttcgata ttccaaaatc aaaggttcat gagtgggcat
    12001 tttaatctcc ttatacagat tttaatccgg ctgagctgaa tcaaatcaca attcgcatct
    12061 ctatgccaat tatggcacga gtatattttt tgtgtgccgg ttctcccgcc aaacaagtcc
    12121 tgccaacagg attgccgtaa gaatcgttat gaccatcccg atcaataaac cgttggtata
    12181 agaaggatcg gggtgatttg ccactgtttt tatggtcaaa tcggtatagg tatacggaaa
    12241 agcctgaaaa gcgttcagta ctttccatgt ttccaggtgg ctccaaaatt ccggaagccc
    12301 gaatagaaga atcagcaaag gaatcgatac cgataaaaca tcaaattttc gtatttccat
    12361 cgattgttgc atggcataga cagcttccgc catcagaaag accagcggca atatcagaaa
    12421 acgcgcactt acacgttcac cggaaaacag cggaatcggc agcagcctga atattccgta
    12481 aaacttcccg attgaaaaaa gtgtcagtac aatcatcgga accaagatcg ccgggtagcc
    12541 ggtttctttc cgtctgtttt tcagccacaa tatgccgaat atcaagagaa aaagggctcc
    12601 ggatacaccg atatagtggt caaattccca ccaccccaga ttaccgccgg taaccgccga
    12661 atttaaggat tcggatgggg gaatgatctg aataaacgac cgaatgagtt gtatcggaga
    12721 ggtaaagccg cctaaaaaat catcatcgaa agccgatgcc tgcagtgccg gcggaatgat
    12781 ccttaccatg gaaagcaaac aagaaaacat tcccgctttc aaaaccgtga acagattctt
    12841 tcgtccggta agcgccaaaa aagctaaaaa aagcagacac cagataaatt gatgaaaaga
    12901 cccttgcaga aacataaaaa acaacagaaa tgaaattttc gccacccatt tccaactgtg
    12961 atcaccgttg atcatccgaa taaccaaata tacaaaccac gggaagagaa aatacccgcc
    13021 ccacgtgata tgtcccacgg cataatgcga agtaatatgc ccgttgaaat aaaacaaaag
    13081 gaaaaaccag gtaaaaccga aaagggaaag atgcatgctt tttttcaagc ggtacagccc
    13141 ccaaaaaccg atggcataca gcagccaagt atgtatcaga ataaaatcac cgacatccaa
    13201 ccacttcagc aataggatct gcgggctgag caatacatcc ggaagcgcca tataacgatc
    13261 cgtcacgccg cgcaaagcgc tcgcgtcctg catatgcagc ggtaacacac ccttcgtcac
    13321 ggcatctttc agaaatgcga ttctgggagc atttacctca gcccaatcgt gaaaatcaaa
    13381 aggaattttt ccgaaattaa gaaaatatcc ccatagaaac atccccaaca gaaacagaaa
    13441 tccgcaccaa atccaactta ataacggatt tgcttcctca atttccggat taacaattcc
    13501 ttcgaaccaa gtgatttttt tattttgttc catatttaat gattgtaaat ttgttgaatc
    13561 agttaagcaa acttttttct tcaccggctg cacaaagaaa atattcctga tcgcttttca
    13621 attccgtccg ttcattcaaa aaagaaactt catcaaaaaa actatatcgg aggcaattct
    13681 aaattcggcg ttcccttaac cgaaatgacg acgcttatga agatcaaagc cgcaccggcc
    13741 gcttgaataa acgaaaaaga ttctctgaaa aacaaaattc cgaaaaaaga ggcaaaaacg
    13801 gcttccagtc ccaaaatgat tgcggcttga gattcatccg catgcttttg acctaccgct
    13861 tgcaacgtaa agccaccacc gatcacaaca atccccgtgt ataagagcaa aggaatcgat
    13921 tctctgacac cgctgagatt ccctcgatca aaaatcaacc agcacagaag attccaaaag
    13981 gcgcatacca aaaattgacc tgccgaaaat cgcatggcat cgactcttcc catcgccttg
    14041 ccgaccacta aaatatgaat tgcccaaaaa acagagccga taaagacaat tccatctccg
    14101 gcggatatgg tactgagagc tttccccgaa gtggatagaa gatacattcc gatcaacgca
    14161 atcgccgccg cccaaaatgc acttcgggtt atcttttgcc gaaaaagcag ccatcccata
    14221 aaaggcacca acaccacata aattgttgta ataaacccgg tatttcccac cgtcgtatat
    14281 cggataccaa attgttgtaa atcggctgcc aggaaaagaa tggtaccgga taagaatgta
    14341 atgcgccaag ccctccgatt ttcaggaagg cgaaaatgag taatgataag aaccgataaa
    14401 gaacctatca gataacgaaa cccgttgaat gcaaaagcgg tcattgaatt cgcacagatt
    14461 ttttgtggaa taaaaccgct gccccaaata atcgctgtca gcaaaaggat cagattggat
    14521 tttttttgca gcgcggattt ctccgcggaa ttcatttcat tttccaacag agcaccccat
    14581 caatttgcaa ttttcctgat gaacatccag ataaaacggg ttgtccgtct caacatccaa
    14641 gggcgatttt tccgtcaaag caatcaggta tttcagattc ttctcaagca gtgataagtc
    14701 ggtaatttcg ccgtgccccg gcaaaaccga ctgaatatcc ggctcggtta accatttctt
    14761 caggtgttta atccaggaag gaacttcttt cggatcatta atgacgggaa tttcttcaac
    14821 actgtcaccg gccagaagca tcttatattc ggggatatag cccaccatcc catcgggtgt
    14881 atgtccgggg aaggaggaca attccaggcg aaaggagcca aaatccagat ttaatttgtc
    14941 cgaaaaaaca aaatcgggag gaacagggca gacgtcttcc catgctttgg agtattttgc
    15001 attaagtttt ttaagcgtcg caggtagttc gaatttaaag cgacggattg cattctgctg
    15061 tgcgataaca ttggaaaaag aaaatccgtt tgtccccaag cagtgatccc aatccgcatg
    15121 agaataagcg gcgacgacaa aatcaaattt ggctaaaagg aaactcatcg cctgcatatc
    15181 cgccggataa gccagcgttt cccatacaag agccatcttc tcccccttca gaattgctcc
    15241 gttaaccaac cagttttgac cgggcatgaa agcgctgcgt gtcaaccata tggatttatt
    15301 ccattctgaa atcatcggca ttcgctgatt ataacggaaa agtcggcaaa gaactctcct
    15361 atttgtctga aacggttcat aacaaggatc attcttagga taaaattcag cttatggcta
    15421 tcgtgattct gcatgcttcc atacgggatt tggaatctgt cctctatctg gaaaaagttt
    15481 gttttgaaga ggatgcttgg accattctgg atttgaccta tgctttatcc gcgcccaata
    15541 aaatccgttt caaagctttg gtggacgaga actttgccgg atttgccgca gccgaaaatc
    15601 atacgaaaga atgtatttcg tggattacaa ccatcgcggt gctccccgaa tatcggaatc
    15661 ggggtgtagc cagtgcatta ttagatgctt gtgaagccga tttatcttat ccgaaggtcc
    15721 gcttggctgt aaatgtccat aatatcgctg cgattcgact ttaccgaaaa tatggatata
    15781 aggaaataga aacctggaaa aattattata tgaacggtga taatgctctg attatggaaa
    15841 agcaattatg aaggttgaaa agaaaagact tgctgcggaa atatttttat cgctcttgat
    15901 tcttctcgga atattttaca gtttttacaa tatgaattgg gattccggtg cactgctcca
    15961 tcccgatgaa tatgggatgg caaataccat cagccagctt tcgatccctt cttcgatcgg
    16021 taactatttt aatacacggg aatcgactat cagtccctat aataagtatg acttaatggg
    16081 gaataaggtc agtgacgggc cggataatcg catgcgatgg gggcaattgc cgatcacttt
    16141 gattcgatta accgctgaag ctttcggtca aacaggctat ctgcagctca gaaaaaccgg
    16201 tcgctatctt tcggcaatca tgaatcttgg gaccttgttc ctgttgtttt tgataacaaa
    16261 gcgcttgttg aacgaccgcc tgatcgcttt actcgcgacg gctctcagca gtctggcggt
    16321 catgcagatt cagcaatctc attttatgac ttcggataat tttgcggttt tttttaccac
    16381 gctgacactt tacgcggcag tgagaatttc acaggaagaa attctcttac gcgatcctca
    16441 aagtggaaaa tacagagtaa cgagagacgg ttggctatgg tttgctttgt tcggcgtatt
    16501 cttagggatg gcggtctcca gtaagattaa tcaagccgtt accggaggaa tgctgctttt
    16561 cgccgttttc ataaatatcg ccgacttgaa gctgacttcc aaaaaagatt tcaatcaaat
    16621 tttcaagctg gcattcggaa tgtgcgtatt cagtgcggtt tgcgcacttc ttacattccg
    16681 cgtgcttcaa ccgatgagtt ttcgttcacc ccacggggat accggctttt ttacctggca
    16741 ttttaataag gattggattg atagtatgct ggtttccatg gcggaatccg gcgggaacgg
    16801 cggcggaccg ccggcagaac aatgggcgca tcggacaccg gtactgttcc ctttgatcaa
    16861 catgattctt tacggaatgg gaactcctct gggattagcc gtttggattt ccgctttagc
    16921 agctgtttgg aaaatgttcc gttcacgcgg tcgcttatgg aaaatattgt taattccgac
    16981 cgcttggtca tttttgtatt ttctttttat gggtacacgg tttgtgaaaa gtatccgcta
    17041 tatgttaccg gtctatccga ccttgtgtct tttagccgct tggggaatcg cttctttatg
    17101 gaggaaatac aacggcagaa agaaaattct tccgggagtg atttccgttt tggttgtggg
    17161 cggagcttgt ttatgggcat ctctcttcgt caaaaccatc tatatgcagc cgcattcccg
    17221 cgtggaagcg gtaacatgga tttatgatca cattcccgcg atgattcaac tggaaggaaa
    17281 gtcggacagt gaaggaacaa tcgtgacaat cgtcccttct gttccttcca ccttggcgat
    17341 tacggcttcc gtaccgttcg aaaccgatat ccgagttcag caaaataccg tgattgaccg
    17401 cctactcatt cctcatgcgg ctaattacac tttctccgac agtaccttgc ggatcgcaat
    17461 cgttcattcg gacggaacga tcctttcaca aacagaaaca tccattcaag cgggaacaaa
    17521 ggatcaagcg atcaaccttt ctttgccggc agcggttttg cttccggccg aaaattatcg
    17581 acttttatta agcattcccc aagggaacgg aatcacttta cggcgtaata ttcttgccaa
    17641 tgaaaattgg gatgaagggt tgccatttcc gctttacggt aaagatccgt ttgggcaact
    17701 ttacaccgga atcaccaatg aaattcgttg gcctgacagt gaagataaaa aagaaatgct
    17761 gttggacgta ttagaccaag ccgattatat tattcttccc agtcaacgag gaatttggag
    17821 cagcgtacgt attccattaa cttacccgat gaccattgac tattatgagt cactgttcga
    17881 tggcagtctc ggattcgaat tagcggcgca gtttcaacgg cctttcaagc tcggaccgct
    17941 ctatttttcc gacttaaccg gtaagttcta ttggaatcaa gcgcctgttc ttccggttaa
    18001 aaatttgagc ccattcgctg ccgaagaagc tttcagcgtc tatgatcatc ctcccgtatg
    18061 gattttccga aaaaccgccg attttgactt atcgaaagca aaagccatct tagatcgtac
    18121 cgatttatcg aaagtaatcg ttcagggacc gacggaagct gtttggccgg aaggatatgc
    18181 cgattcagaa gaatagttcc gaagcgaaac aaaatcgccg attttttcac cgcatactgt
    18241 aaagaaatcg gcggaacttt cttgtcagtt ccggaaagga ctgctataat gctgtttgca
    18301 cctggggcgc atagctcagt tggttagagc gcttgtatca cacacaagag gtccgggggt
    18361 tcgagtccct ttgcgcccac ttttttcttc ctttttatcc tacaatcgat ccatttgatt
    18421 caccacagca gtgaaagttg ttttatatgg aaattacttt ttcggctttg aattgggcgt
    18481 cgataatatc gctcatacct ccgacaatcc caaccgcgac ctgatcgacc aagttgtaaa
    18541 agttcaggca ggtggaacac agaattaagc gcacgccctt cttttcgagg gattttaaag
    18601 tatcgatgac cggtgatccg tttgtcgcca actttacgcc gtcagtataa agacaaatcg
    18661 ctgccggcag aatattattt tcatcgatca acttaagata aaccgaaatc agttttaatt
    18721 gcagttcggg agtcccttca cccatcccgt tccgggtaat taaaataacc gtttccgacg
    18781 caggaattga catatgattc tccttataga tccgattgcc ggttgagatt ttttataaag
    18841 aaccgaccga atttcaaacg gcaatcatcc aaataattga aaatacttgg gaaatattat
    18901 acccgactaa tactcattca taaaatattt ttattttaaa tttaagaaag aagtcttatc
    18961 gatttttttg agtggataga acaatgatcg aaattcattt caatcggaaa tcataaatgc
    19021 cgtcgataat cggataatcc cgaccgcaag aatggcaatg cactttttcg gactcttcga
    19081 caatttcgta gctatggcaa ttcggacatt taaaaaattc ccattcgact gcgtccggtt
    19141 tctccgacga ctgtcgattg cgaatgaaaa tactcggact cagcttgaac ggggaagcta
    19201 aatattgcag ggcggattcc agcttcaaca tccagcccag atttttttct ttcttcagcg
    19261 attcgattcg taaatttgaa acagtcaata ccttttccgg tataaacccg ttttctgtca
    19321 gtacttttct tacggtatcg gggtgattat caaagttcag ttcggcgaac tccacttgtt
    19381 cgggagaaaa aggattccaa tcctgtttac cggttaaata tcgaaaaatc gccttcaaat
    19441 ttcttttatt ggcaaattcc aaaataaaaa cggactgata ttgcataacc cgttggatct
    19501 cagccaaagc cgaattaatt tcactcagat gatgcagcac gcgaatcatc gtagccccgt
    19561 cgaacagccc gtccgcaaac ggcatatggt aaacatcggc agtaacatag ataaaccggt
    19621 cggactttcc caatcgctgt tgagcttgct gcagctgagt gagggcgtaa tccatcagaa
    19681 caatccgttc gtatccccga taacgtaacg tattccgccc ggctccggca ccgatctcca
    19741 acatcaatcg gccggatttc ggcagcaagc gttccaaagc aatggcttcg gaagcgtctt
    19801 catatgcccg cttcccttcg ccccaaaacc ggttttggta atcggattcg tcgtaattgc
    19861 aaaaaattgg agaattattt tcggacataa atcctttatc aatcaattaa aaaatgcgtc
    19921 cggacaggtt tgccggcggc gcccgaatga tccaccttat cgctgcttcc tctcggacct
    19981 gacgaggttc gatggtatcc gccgcgccgg tcccgcccga gcgcctttta agaataccct
    20041 aagcgattat tcatgtcaat ataaagatca caaaagaaag ttacatccga tatccagtca
    20101 tccgtcagga atttttcata atgatattcc cgacttcatt ggcaatatct tcggccgtat
    20161 ccaaaatccc ttcaaatttc gtaaacattt ctttctgacc gatcagataa cgggcgtcat
    20221 gttcctcttc gaatacctta tacataaact catggaaaag gatatcgcca ttctcttcga
    20281 gccggttgat atcgataata tatttatgaa tcgtaagatc ttttttgaaa tcgctgaaaa
    20341 cactcaaaag tttcatcacc tgatgaaccg attgatcgat gacatccata aattgcatca
    20401 tcgcctgatc aatcgtctta acatgatagg tgtaaagcaa aatcggaata tcttcgagat
    20461 aatcggtcaa gtcatctaaa agttgtgtga ttacaacaat atcttctctt tcaatcggag
    20521 gaataaattc cttggaaagt ttttccatta tttcatgatg cttgatatcc gccgtatgtt
    20581 ccagtaagtg aacttcatcc atagaagtac gcaaattgat tgtgttatag ttttcgaaaa
    20641 tatttttgac cttctcaaca atttggatgg aaaattcaga ttgttcaacg aaagcattaa
    20701 agtagaaatc attacttttt gaagccatga tatccattcc tttcccacat cagaataaat
    20761 agataaacag ctttactacc agaaagccga tcaacccaca acccggaaat gttaaaaccc
    20821 aagcccagac catatcctta acaattcccc aattcacaga cgaaatcctt ttccccgcac
    20881 ctactcccat aatcgctgtg gttttcgtgt gtgtggtact gacgggaagg ctgaaaagag
    20941 aagagatcag caaacaactg gctgcggcaa tatccgaggc aaagccttgg aatggttcga
    21001 gttttaccat attcatcccg accgatttga taatgctgta cccaccaatc gaggtgccca
    21061 aagacataac caatgaacat aaaatcatca accacaaagg aacaacaaaa acggattgcc
    21121 ccgtttctcc ttgagacaat gcgatgccca gcataaagac agcgataaat ttttgtccgt
    21181 cttgagctcc gtgcataaaa gccattgcgc cgcttgcaaa aacctgggca tggcggaaaa
    21241 atttttgcgt cctccggcga tcaatgcttt gaaaaatcaa tttcaccaat ttataggcta
    21301 agaaaccgaa accgaaaccc ataaccgttg acaaaaccaa tccgtaaatg accttgatcc
    21361 attcacttcc gttgattccg gcaaagctgc catgcagacc gatagcggct cccgaaagac
    21421 cggcgatcaa tgcatgactt tcgctggttg gaattcccat gaaagatgcg accgttgccc
    21481 aaaggacaat cgcaaccatt ccggcgcaca gtgccagagt ggcttcgctt gaattggtcc
    21541 cgaaatcaac cattttataa acagtttgag caacggtaga attcaaaata gtcatcacaa
    21601 agacgcctaa aaaattaaag accgcagcca taatcagggc atattgaggt ttcagagagc
    21661 gggtaccgat acaagttgcg atagcattcg gtgcatccgt ccaaccgttc acaatgatca
    21721 cactaagtgt caaaataacg gttatcagaa ggggaatatt atgagaaata aggtagataa
    21781 actcggaaaa agatatggac attacaatac cgatttttgc acccgattat ttgatatgaa
    21841 acgctgctta ttttaatgca ataataacac gtaaagcgaa cttgctgacg gttttctaca
    21901 ctattctgtt t
//
LOCUS       DAOSMM010000037        21001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2528_length_21001_cov_16.785305, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000037 DAOSMM010000000
VERSION     DAOSMM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_21001_cov_16.785305"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..140)
                     /locus_tag="PK898_06525"
     tRNA            complement(68..140)
                     /locus_tag="PK898_06525"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Asp,seq:gtc)
     gene            complement(264..1178)
                     /locus_tag="PK898_06530"
     CDS             complement(264..1178)
                     /locus_tag="PK898_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOU19724.1"
                     /translation="MEIKTITIIGQGALGILFGSQLAKHLGNQNVRFLADRERIDRYQ
                     KEGIYCNGEKCDFTYITPDLQGNPADLLIFSVKYPALPQAIRDAKNQVGDKTIILSLL
                     NGITSEQLIGDTYGMDHMLYCVAQGMDATRTGNRLVYSSAGLLEFGEIDNTLSEKVKA
                     VETLFKKADIQYRIPEDMNRIMWKKFMMNVGVNQVTAAYNSDYGAVQAKGEQRDTMIA
                     AMREVIQIADRKGIHLTEEDIQYWLNVLDGFKPELMPSMRQDTAAKRKTEVELFSGAM
                     IRMGKELDVPVPVNEMLYKKIREIESAY"
     gene            complement(1366..2484)
                     /locus_tag="PK898_06535"
     CDS             complement(1366..2484)
                     /locus_tag="PK898_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19725.1"
                     /translation="MEFTLPVYHEPDFSQAMFVHSPDVNTEQVIKDGVAPDNFHGTSM
                     FPEYFKINGEWKLAEDSRMDCCVVIRENGKLEVIELRNLKIGDRVILGRTDHAEEGIF
                     LHNSGFTVTEEINEKFLFRAGRSRETAFSKDYDSLYHLLLHEKEFGYIVWVMGPACAF
                     DADSRNAMSQLICNGYAHGLLAGNALATHDLEAAYFGTALGQDIYTQESKPNGHYHHI
                     ELINRVRRSGSIRKFIDENQINNGIIASCIRREIPIVLTGSIRDDGPLPEVIADVYQG
                     QDAMRNIIRKATTVICLATQLHTIATGNMTPCFRVVNGKVRPLYIYSVDISEFALNKL
                     SDRGSLTATSIVTNVQDFVVMVANGLQKLEEKPFQSCL"
     gene            complement(2504..3094)
                     /locus_tag="PK898_06540"
     CDS             complement(2504..3094)
                     /locus_tag="PK898_06540"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOU19726.1"
                     /translation="MAEQPINQKLSRFQEIRSNKKIRIGLIIFVLSALTILFIKSDFE
                     TIKNFIRDHQILGIAVSLLLYILFGVFFIPTEPLTIFLGALMGVLQSVIICIVGNTLA
                     SLIEYYIGSLIGDISDFEKQKENLPLKLGKMPVKSLPFQFGVRAIPGIGSKFVSVASG
                     IYKVSIPYFLAASVISNFIGAIFLSYGGDFLARYFK"
     gene            3275..4261
                     /gene="fni"
                     /locus_tag="PK898_06545"
     CDS             3275..4261
                     /gene="fni"
                     /locus_tag="PK898_06545"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOU19727.1"
                     /translation="MSIRSRKENHLNACLNSDVQSEISNGFENYRLINESCPEEDFSA
                     LNFETDFLNHKISFPLMISSMTGGTEQSEQINRNLARAAQATNLPFALGSQRIYLVNQ
                     KLETLKYIREEAPDIPILANIGAVQLNYGWTKNELLKLVDSVNANALILHFNPLQEVF
                     QPEGNTDFRGLIDKIKELCADFPVPIVAKEVGFGISVSTAAKLADAGIRWIDVAGAGG
                     TSWAKIEALSAGQKIPAETIAPFGGWGIPTAVCIDQIHQKMPEINLIASGGIRNGIEM
                     RKACLLGAKLCGIAIPLLRPALENAEAVITVLERYIFQYRAAVFTSSAVEKI"
     gene            4315..5313
                     /locus_tag="PK898_06550"
     CDS             4315..5313
                     /locus_tag="PK898_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU19728.1"
                     /translation="MSDFQKEIIRQINETLYRQIAKEEIEKKDQTLSDMLFYALGMDS
                     DGTVHAGGKRIRPLICCLTCGAISGSHEPALLPGTVLEMFHNFTLIHDDIEDLGETRH
                     GQPAVWKKWGSALSLNAGDMLFALTYKTLHEISGRSKTGSEISVFDQMVTDLIMGQHL
                     DISFEKRQSITEEEYLTMISGKTVALIKGSFTLGAIAAEANQSTIAELEKVGEKIGLA
                     FQMQDDYLGIWGDSNHLGKSVSTDITDKKKSLPIQYAMNHDASFNKEWQEYNGDPERV
                     GYFADRLREIGADSYTRNQWETAMSEASKKMKSFSFNEKYGNELNELIGTLAGRNK"
     gene            5310..5900
                     /gene="folE"
                     /locus_tag="PK898_06555"
     CDS             5310..5900
                     /gene="folE"
                     /locus_tag="PK898_06555"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOU19729.1"
                     /translation="MIDEQKEKNPTIRDAIEIILTQLGENPEREGLKATPIRVEKMYR
                     ETCSGYQQNLKEIVNGAVFHEKSNGMVLVKDIEFYSLCEHHMLPFFGMVHVAYLPDEK
                     IIGLSKIPRIVDMYARRLQVQERLTTQIMEAIEEILQPKGCGVLIEASHLCAMMRGVK
                     KDQAKLITTNFSGLFLKDESIRSDFFNQVNQESVKV"
     gene            5991..7169
                     /locus_tag="PK898_06560"
     CDS             5991..7169
                     /locus_tag="PK898_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU19730.1"
                     /translation="MDIFQKCYEYDMIKKMIAEGHYPYFLPLSENEGTVAEYKGKKLI
                     MCGSNNYLGLTTHPKVREAAIAAIHQYGTSCTGSRFLNGSLELHETLEHEIAEFVGKE
                     AALVFSTGMQTNLGTISALVGRNDYAILDKDSHASIYDGVKLSYGKLERFAHNDMEHL
                     EKVLNSLPEEAGKLIIVDGLFSMEGDLAPLDKIIPIARKYGARLMVDDAHGIGVTGNG
                     HGTAAQFGLTKEVDLIMSTFSKSFASLGGFISGDEEIVHYIKHNARSMIFSASIQPAS
                     AAAALAALRIMREEPEWNQRLIKNGNKMRKGLSELGFNIGNSVTPVVPILIGNNEQTF
                     EMASGLFERGVFVNSVISPATPPGRQLLRTSYMATHTDEQLDQVLDAFEYVGKKVGLI
                     "
     gene            7169..8410
                     /locus_tag="PK898_06565"
     CDS             7169..8410
                     /locus_tag="PK898_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19731.1"
                     /translation="MGKNSEKSEYRVLLFDIDGVLVSQGGYRKAFTDTVSWFMNYFGF
                     PEIQIGREFADRFDAYGVLAEWDMVPLTLSFFLDWYAENNPQARPFGKLEKLNNLPRI
                     HPAMPFPEVLWQKIPQIQDILNGEDIPALAVYKHCLDQKDQSILPRLWDDSVLSEFLA
                     DSLNVRTSWCFQKLETYILGSELFEKTFHIPTEKGIPSGLMTVNKALLNEKYRKKILE
                     MNGKSLYVATITARPNLLPACIKDDKTEIISAIPEAEIAMDELGWGDERIKNVGVGIL
                     HYYEKKRDLPQDSRLKPNPFHALFAILYAISDSFEKTFNVVSTWDFPEKSENKINPFL
                     PILPEGSMLDIAVFEDSLSGIQSATDAAKILQNYGYIAQTRRYGIYTNPEKAELMKTI
                     GTKCFPTINDALDQYFSETDQ"
     gene            complement(8404..9681)
                     /gene="fliB"
                     /locus_tag="PK898_06570"
     CDS             complement(8404..9681)
                     /gene="fliB"
                     /locus_tag="PK898_06570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HOU19732.1"
                     /translation="MENYYQTSCCYSPSWMKEFKCLAENCPQTCCQGWNIPVDEKHAS
                     FYQNLADKNLHEITDSALRKISTGKGKNRSTEYFLNLLKQPDEICPFLSEAKFCRLQE
                     KYGEFALCDTCFFFPRYYWQIDSSLFLSASLSCPKVRELALLNPNRMDFESIETYIDP
                     HADWLFCEFIRDDKSRILLKLRDDIVKALIKCVQNRGRKMPDRLANACNLLISIGEMI
                     ENGMDEEAIRNILIGPSSGELSDPFFTSGECEDPVRLSEWIKVLNECLNWGLEGASKS
                     SIAIQRNMISLLAGETDPIILMAEHYRMGRDNMMELFSQKYAHLPENFMVLFFFSDCL
                     KQFSQYQQESITVDRIVKFEIAQVVIVWSLLKIQAVNKALQIGSLTVDSFLQIAAETD
                     RDFLHYPAYIRQCAQRLSMKFDSNEKLIQCLFY"
     gene            9834..10583
                     /locus_tag="PK898_06575"
     CDS             9834..10583
                     /locus_tag="PK898_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19733.1"
                     /translation="MKKVLFKKRFSAIILAGFLIVTILGFIPSSKQIVWSTQTPQSGS
                     SATGEVIKMRNPVIRSGAVIILKNPVGTVVPTSVQKSNPSIQLLQTSTPMPVIPPVIW
                     QVTPTLGRAVEVIPTGMNVPKPAYDCNVQVNSPYWYQQFAPGEDFDFDVTITNTGTEA
                     WNTDIDVMQYTGWRMEIEGKYLYDLDKDYDSAQIVLPGQSIRWKIRMEAPKEKTTEDD
                     KYYTTYSLVKGTDYDNSRFCPFSLYIYVPHK"
     gene            complement(10674..11279)
                     /gene="recR"
                     /locus_tag="PK898_06580"
     CDS             complement(10674..11279)
                     /gene="recR"
                     /locus_tag="PK898_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOU19734.1"
                     /translation="MANIPAPVQNLIDAFSMLPGIGPRTASRLTFFLLKNNTDIAQKL
                     STALMNLKTETALCKECFNITLAGHDLCAICENDQRDQHELCVVETPMDVIVLEQSGG
                     YFGKYHVLHGLLSPIEGINPEDIRLAPLFERVATGDYKEVIIATNPSMEGDATALYIR
                     QRLEPYHVRITRLARGLPSGGELEYADQNTLLRALSGRQEM"
     gene            complement(11284..11634)
                     /locus_tag="PK898_06585"
     CDS             complement(11284..11634)
                     /locus_tag="PK898_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOU19735.1"
                     /translation="MAKGYGRPGGRPAGNQNAMRQQLIELQKQMEEEQAKIAEATVTA
                     TVGGGAVKITMTGDQNCKSVEIDPDFLKDADAEMLQDMILSGINLALEQSRKLSEEKM
                     SSISNTLSSMGLGF"
     gene            complement(11767..13356)
                     /gene="dnaX"
                     /locus_tag="PK898_06590"
     CDS             complement(11767..13356)
                     /gene="dnaX"
                     /locus_tag="PK898_06590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOU19736.1"
                     /translation="MSTQALYRKWRPRTWDEFVGQEQVIRVIRNAIASDRLTHAYLFS
                     GPRGTGKTTIARMLARAANCTDPVLANRPCDHCENCLSILDGSFMDIIEIDAASNTGV
                     DDIRDLRDKINFLPAQGRMKVYIIDEVHMLSTAAFNALLKTLEEPPAHAMFILATTEI
                     QKIPATVLSRCQHHEFRRFPVTEIVGWLKHICEKENFDYEEEALTLIARQATGAMRDA
                     VSLLDQLSSTGDKITLQAAQSILGVSSNQAVIQLVDAIIDHNAGLGLDILHQALDSGS
                     DPRQFARQMVEHLRMILVIQMGSRKPLEITAEIQSILAKQSAKLTVDQVIEMIRLFDQ
                     AVTDSRTGWYPGLSLELATAESCEEKKPVQPVFIQSPVINDQPAKKAASVLPDEEELT
                     EEADDYAGAKVFIRKGQQPDPSVTKEEIEKNWTKVRELIKKHDAILDATLNHAQLLEV
                     RDGVLYLGFPRETIKNQMENSRKSMLWTSAAISNVLGKPVGVALTILGKKNRQPESQG
                     PLVNAALQLGGKLVINKEKTK"
     gene            complement(13506..14117)
                     /locus_tag="PK898_06595"
     CDS             complement(13506..14117)
                     /locus_tag="PK898_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU19737.1"
                     /translation="MKTFWKILILLLVNVIVSVTATFAVLYYWENIRNAEKPYTLITV
                     NDSASPVVSSPTIEIDYNILMTPTSPIQVNNPINSSATSQVTADVTSQPTIPPVRDLL
                     VSISDVIGTGDINSEAVYIKSNADTAVQLENWTLEDADGNVYTFPNIQIIRKGIIVPL
                     YSRSGHNTPFELYWGLTEAAWQSGETVVLKDPDGNIQATYRIP"
     gene            14322..14825
                     /locus_tag="PK898_06600"
     CDS             14322..14825
                     /locus_tag="PK898_06600"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOU19738.1"
                     /translation="MVLYIDGHNLIPKIPGMSLRQIDDEEHLIELLQEYSRISRKKVE
                     VFFDRAAPGFNGDKTAGTILVHYISEKTTADEAIIRRVRAWGKRSKEITVVSSDHHVQ
                     NQVKALGAGMMTSDQFAREIVYLSQQSQPKNNKKDEPQPERPLSEKEIREWMDLFNSG
                     PSEKYSK"
     gene            complement(14791..15267)
                     /locus_tag="PK898_06605"
     CDS             complement(14791..15267)
                     /locus_tag="PK898_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOU19739.1"
                     /translation="MKKIPLLILCLVITAGFFGFCCVNQIQQDYAGIYRGAIHLPAFF
                     PSENVLIAVQIILLALVGIAVWFILERQINPNRKKNVLINTLILLGLIFCWNFLMFNT
                     GNLTGALVLVIAGIILGLIVLSMAWLVDHRAGYLMIPINVWIIYLIILNISLTVLN"
     gene            complement(15287..16942)
                     /locus_tag="PK898_06610"
     CDS             complement(15287..16942)
                     /locus_tag="PK898_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19740.1"
                     /translation="MKSVRNSPAKLFLYLFAIVLSSGAIARGIDTDWGAVLIHKVPVE
                     ITEYQFAEGKIYRPITAQSLNERPAILLINALNPDNEALSSLASELARRGFVALSIDL
                     SIQGDSVESTRNNLMEILSAGFQFLESQSFVLKDYIGLAAYSIPTLDQPAEKFIQENF
                     RSVVFIASAPNLLSDYQNVENKPYFLRINSPFDEYQNYQIDQTASPDEQFFAIHPFAP
                     IDSKVISQTLNRFHQTLKISNDSPLWFDAGKQLSTLREISLSVGFVALLLTLIPLSQM
                     IGKKHLLEMPSEYSKKDINPKPFSFSRQIGGSFCGFLLLLITYWIFLRFSQRAENQLN
                     VSDGIVIWAGTASFIMLVSGFFSKKGTLRKQQLKFVDLFLSFIRGIVICLILYGIVWI
                     FSRVFMIEFRFIVPGLRPFSDINKTRLSLYSLFFCLFFWLHATWPKQKNFLFDNFIPI
                     GAFLLFLAVQFFPVLFGGTPGLEFLTRQFSAVAGLPSVTTDSFFTLCLGAMTDTVMYS
                     FIIFSLLLIIQNRMKRSINNNIISAIICGCVCSWLLYNGTIII"
     gene            complement(16958..17668)
                     /locus_tag="PK898_06615"
     CDS             complement(16958..17668)
                     /locus_tag="PK898_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19741.1"
                     /translation="MKKNLLIVFFLLIFLEVTAVYAENPRVQCGNGTVTIKMSVPSTN
                     VRCLTVMNSEKAAEGKSLSEARISSAAVVIDDYKVEGKFSPEVTAFSMNGLSNVNSEI
                     YQKAVDLTTMINKINGGEGINAVTAPIPFLPYQDKTQLFVAMPEIINFISGTAIRFIT
                     AYGDSGAMVDNGNLLYTVQGISQDGKYYLSITIPISNSQIASPINPAGFDWNSLPPES
                     WKPSLTELDEMAKSVSLQ"
     gene            complement(17849..19516)
                     /locus_tag="PK898_06620"
     CDS             complement(17849..19516)
                     /locus_tag="PK898_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOU19742.1"
                     /translation="MNDKIDYEPVSNDKAHLKNVAEWEASKVITPEIMETARKILLRV
                     IDGADVFKTIRANPVPKHGNISKDALVKAYREMVANGEIQEDPHLLAKIRMKPNRTLS
                     GVSTVTVLTRPFPCPGNCIFCPNDARMPKSYLPDEPGAARAFQNHFDPFAQVRSRLDT
                     YQALGHPTDKVELLILGGSWTSYPEDYQAWFVQRCLDAMNESDSESLLIAQQQNETAA
                     HRNVGLVVETRPDEITESNLKYLRKLGVTKIQIGIQSLDDEILNLNRRGHTVQQALEA
                     SKLCRAAGFKLVMHWMPNLLGSTPQSDREDFMRLWVEADQGLGFCPDELKIYPTQLVE
                     NSDLYEVWQAGKYLPYDTETLIDLIAGIKPFIPPYCRVNRIIRDIPSHHIVAGNTRSS
                     LRMDIHQRMAENGTRCNCIRCREIRDQAVDPTTLKLVDYSYHAAEAEEHFLQFVTPED
                     KIAGYLRLSLPTRSSPNLDIADLNEAAIIREIHVYGQELPVGMELKGAAQHAGLGKKL
                     INKAIQIAKNNNFKKLAVISAIGTRKYYESRGFSRGEYYMVMDLTNK"
     gene            complement(19506..>21001)
                     /locus_tag="PK898_06625"
     CDS             complement(19506..>21001)
                     /locus_tag="PK898_06625"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU19743.1"
                     /translation="NLNGLIISGMKKATYFSYLLFLPFFLLTIVQNQVFANSVIEEKP
                     AKIVYALAAPFTTITDNISLRDFSDFWSGTSASQPETIPGKLLIVTSDPERFRTLFGE
                     GDKNQIEFVTDIHAAFAQSINNGNWLIVPFEQLDARFKVISIDEQNPLHKDFNDNLWP
                     LTIRINGNENMTEQDWAAVQPLNRDPSKLTTMILTGVTALVRGTAAYMDVWGPEYPAS
                     NIGDTLREADILHVNNEVPFAKVCQQTESELARLVFCSKTSALELLKSIGTDVVELDG
                     DHFQDYGDDAVYYTLKLYKDANIPYYGGGANLKEAQKPLIVEDHGNKFAFIGCNGKEI
                     GYAVASDTRPGAAHCDIPLIVSQIKELKASGILPIVTFQHIELYKIYPNEDMAADFKA
                     VADAGAVIVSGSQSHIPMAFDISKDSFIHYGLGNLFFDQAFFLPETSEATMDRHVFYN
                     GKHISTEILTIKFTNNALNRFMTESERTAVLNRIFAESHIELDQDER"
BASE COUNT         6079 a         4314 c         4558 g         6050 t
ORIGIN      
        1 tgaattaaat gcggtaaata aaaaaagata caaacccact aatttgtatc tttttttatt
       61 tttgttgcgg gagcgacggg gttcgaaccc gcgatctcgg ccttgacagg gccgcatgtt
      121 agccgctaca ccacgccccc atgagcgatc cggcttgaaa gtgccttccc aaactcaacg
      181 gattggattt taacacagga ttgtaagcag gtcaaggcaa gttctttata atgttgttat
      241 tcaagccgtc tcttgatctc cggtcaatat gccgattcga tttcccgaat ctttttgtag
      301 agcatttcgt tgacggggac cggaacgtcc agttctttgc ccattcggat catcgcgcct
      361 gagaaaagtt ctacttctgt ctttcttttt gcggcggtat cctgtcgcat agaaggcatt
      421 aattccggct taaatccatc cagtacattt agccaatatt gaatgtcttc ttcggttaag
      481 tgaattccct tccgatcggc gatttgaatt acttcccgca tggcggcgat catcgtatcc
      541 ctttgttcac ccttcgcttg aaccgcccca taatcggaat tgtaagcggc ggtcacttga
      601 ttcactccga cattcatcat gaactttttc cacataattc gattcatatc ctcgggaatg
      661 cggtattgaa tatcggcttt tttgaaaaga gtttccacag cttttacctt ctcactcaag
      721 gtattatcga tttcaccgaa ttcgagtaga cctgctgatg aatagacgag ccggtttcct
      781 gtgcgagtcg catccattcc ctgcgccacg caataaagca tatgatccat accgtaggta
      841 tctccgatca attgttcact ggttattccg ttcaacaatg aaagaataat tgttttatcc
      901 cccacttgat ttttagcatc gcgaatcgcc tgaggcaggg cgggatattt gacggaaaaa
      961 attaataaat cagccggatt cccttgcaaa tcaggagtga tataagtaaa atcgcatttt
     1021 tccccgttgc agtaaatccc ttctttttga tatcgatcga tacgttctcg atccgccaga
     1081 aaccttacgt tttgattccc aaggtgcttt gccaattggc tgccgaaaag aataccgaga
     1141 gcgccttggc cgatgattgt tattgttttg atttccataa ttttttccgt ttcctgtcaa
     1201 aagtgttcaa ataatgaaaa attccttcag actgttgcct ttctataacc gaaatgatgg
     1261 ctccattata ttccggaaac gggaatgatt aaatgcccgg aaattacgaa accttggcac
     1321 aaagctaccg gcggcttaaa ataactttcc gctctccggc ttggcttaca aacatgattg
     1381 aaaaggtttt tcttcgagtt tttgtaaacc gtttgcgacc attacaacga aatcctgcac
     1441 atttgttacg atgctggtgg cggtcagact gccgcgatcg ctcagtttgt tcaatgcaaa
     1501 ttcggaaata tcgacggaat agatataaag cggtcggact tttccattta ccactcgaaa
     1561 acagggcgtc atattgccgg tggcaatcgt atgcaactga gttgccaaac agatcacggt
     1621 tgtggctttt cgaatgatgt tccgcatggc atcttgcccc tgatagacat ccgcgataac
     1681 ttccggaagc ggaccgtcat ctcggatgga tcccgtcaga acgatcggaa tctctcgccg
     1741 gatacaagag gcgataatac cgttattgat ttgattttcg tcgataaatt ttcgaatcga
     1801 accgctgcgt ctgacacgat taattaactc gatatgatga taatgaccgt tcggtttcga
     1861 ttcctgcgta tagatatctt gacctaatgc ggttccgaaa taagcggctt ctaaatcatg
     1921 tgtcgccaga gcatttcccg ccaacaaacc gtgcgcataa ccgttgcata tcaattgcga
     1981 cattgcattt cgagaatcgg catcgaaagc gcaggcaggt cccatcaccc atacaatata
     2041 tccgaattct ttttcatgga gcagcagatg atacagggaa tcataatctt tcgaaaaggc
     2101 ggtttcacgg cttctgcctg cccgaaataa gaatttttca tttatctctt cagtgacggt
     2161 aaagccggaa ttatgaagaa atataccttc ttccgcatga tccgttcgac cgagaatgac
     2221 tcgatctccg atttttaaat ttcgaagttc aattacttca agttttccat tttcgcgaat
     2281 cactacgcaa caatccatcc gactgtcttc cgcaagcttc cattctccgt tgattttaaa
     2341 atattcgggg aacatagaag ttccgtgaaa attatccgga gcgactccgt ctttgatcac
     2401 ttgttcggtg tttacatcgg gcgaatgaac aaacatcgcc tgagaaaaat ccggttcatg
     2461 atagacggga agagtgaatt ccatgatgtt ctcctttgaa aaattattta aaataacgag
     2521 ctaagaaatc tccgccgtag gaaagaaaaa tggctccgat aaaattcgat atgacactgg
     2581 ccgcgaggaa gtaagggatg gaaactttat aaattccact tgcgacactc acaaattttg
     2641 atccgatccc cggaatagcc ctgactccga actgaaaagg taaagatttt acaggcattt
     2701 tccccagttt taagggtaag ttttcttttt gcttttcaaa atcggagata tctccgatca
     2761 aacttccgat atagtattca atcaaagatg caagagtatt tcccacgatg cagataatga
     2821 cggattgtaa gacacccatt aatgcgccga gaaaaattgt taaaggttcg gttggaataa
     2881 aaaatacccc gaatagaata taaagcagta gactgacagc aataccgagg atttggtgat
     2941 cccggataaa attttttatt gtttcaaagt ctgatttgat aaatagaatt gtaagagccg
     3001 acagtacaaa aataattaat ccgattctga tttttttatt gcttctgatt tcttggaatc
     3061 gggatagttt ctggttgatt ggttgttctg ccatgaggga gcgatttcct gatttatata
     3121 agatattgat ttatcttatt ctatcattga atcaatgaaa aaaattcata gttataatta
     3181 tagatctata taagatataa tatattttaa ttcaatccgg gcaatccgat gaaggcggat
     3241 tcatggtaaa ctgatgggag aaaaaaggaa gagaatgtca atccgttcaa gaaaagaaaa
     3301 tcatttgaat gcatgcctga actccgatgt tcaatcggag atttccaacg gctttgaaaa
     3361 ttaccggctg attaacgaat cttgtccgga agaagatttt agcgctttga attttgaaac
     3421 agatttttta aaccataaaa tttcatttcc tttaatgatt tcctctatga ccggtggaac
     3481 ggaacaaagt gagcaaatca atcggaatct ggcacgggca gcgcaagcga cgaatcttcc
     3541 ttttgcgttg ggctcacagc gcatctacct ggtaaatcaa aaattggaaa cattgaaata
     3601 tattcgggaa gaagcgccag atatcccgat tttggcaaat atcggtgcgg ttcaattgaa
     3661 ttacgggtgg acaaaaaacg agttattaaa attggtcgat tcggtcaacg caaatgcgtt
     3721 gatcctgcac tttaatcctc tgcaagaagt ttttcagcct gaaggaaata ccgacttcag
     3781 gggattgatc gataaaatta aagaactttg cgccgatttc cccgttccga ttgtggcgaa
     3841 agaagtcgga ttcggaatat ctgtttcgac ggcggcaaaa ctggctgatg caggaatccg
     3901 atggatcgat gtagcgggtg ctgggggaac ttcgtgggca aaaatcgaag ctttgagtgc
     3961 cggtcagaaa attccggctg aaacaattgc ccctttcggt ggatggggga ttccgacggc
     4021 tgtttgtatc gatcaaattc atcaaaagat gcccgaaatc aatttgattg catccggtgg
     4081 aattcgtaat ggcattgaaa tgcgaaaagc ttgcttgctc ggcgcaaaac tttgcggaat
     4141 agcgattccg ttattgagac ccgcgttgga aaacgcggaa gcggtgataa ctgttttgga
     4201 acgatatatc tttcaatatc gtgcggctgt ttttacatca tccgccgttg aaaaaatata
     4261 gaatttttca atagagaaaa aattggaccc gatatcaacc agggagaaac aattttgagc
     4321 gatttccaaa aagaaataat ccgacaaata aatgaaacac tgtatcgaca gattgcgaaa
     4381 gaagaaatcg aaaaaaaaga tcagacgctt tccgatatgc ttttttatgc gctcggaatg
     4441 gattcggatg gaacagtaca tgccggcggg aaacgaattc ggccgttgat ctgttgctta
     4501 acttgcggcg cgatttccgg tagtcatgaa ccggctttgt tgcccgggac agttctcgaa
     4561 atgtttcata atttcacgct tatccatgat gatattgaag atttgggaga aaccagacat
     4621 ggccaaccgg ctgtgtggaa aaaatggggc agcgcgttga gtctgaatgc cggcgatatg
     4681 ctgttcgctc tgacgtataa gacattgcat gaaattagtg gtcgttcaaa aaccggctcg
     4741 gaaatttctg tgtttgatca aatggtgacg gatttgataa tggggcaaca tttggatatt
     4801 tcttttgaaa aacgtcaaag tattaccgaa gaggaatatt taacaatgat ttcggggaag
     4861 acggtggcat taatcaaagg aagttttaca ttgggtgcga tcgcagccga agcaaatcaa
     4921 agtacaattg ctgaattgga aaaagtgggt gaaaaaatcg gattagcatt ccaaatgcaa
     4981 gatgattatc tcggaatttg gggtgattca aaccatttgg gaaaatcggt ttcgaccgac
     5041 attaccgaca agaagaagtc tttgccgatt caatacgcaa tgaatcatga tgcttcattc
     5101 aataaagaat ggcaagaata taacggtgat ccggaaaggg tgggatattt tgccgatcga
     5161 ttgcgggaaa tcggtgcgga tagctatacc cgaaatcaat gggagacggc tatgagtgaa
     5221 gcctcaaaga aaatgaaaag tttttctttc aatgaaaaat atgggaatga attaaatgag
     5281 ttgataggaa ctttagcggg gcgaaataaa tgattgatga acaaaaagaa aaaaatccga
     5341 ccattcggga tgccatagag attatcttga cccaattggg agagaaccct gagcgggaag
     5401 ggttgaaagc tacgccgatc cgagttgaaa aaatgtatcg agaaacttgc tcgggatacc
     5461 aacaaaattt gaaagaaatc gtcaacggtg ctgtctttca tgaaaaatcg aacggtatgg
     5521 ttctggtaaa ggatattgag ttttacagtc tttgtgaaca tcatatgctg ccttttttcg
     5581 gaatggtaca tgttgcatat ttgcccgacg aaaaaattat cggcttgtct aaaattccac
     5641 ggattgtgga tatgtacgca cgccgtcttc aagttcaaga gagattgacg acccaaatca
     5701 tggaagcaat cgaagaaatt ctgcaaccga aagggtgtgg ggtactgatt gaggcgagcc
     5761 atttatgtgc gatgatgcga ggggtaaaaa aagatcaggc gaagcttata acgacaaatt
     5821 tcagcggttt atttctgaaa gacgaatcga ttcgctctga tttttttaat caggtaaatc
     5881 aagaatctgt taaagtgtaa tttccgaaat ttttggaaac atcatttata attttcaaga
     5941 ctttttctgt gcaatcatca tcaatttatc gaagaaaata agaggatttt atggatattt
     6001 tccaaaagtg ttacgaatat gacatgatca aaaaaatgat tgccgaagga cattatcctt
     6061 attttttgcc gctttcagaa aatgaaggaa ccgttgcgga atataagggc aagaaactga
     6121 tcatgtgtgg ttccaataat tacttggggc tcacgactca tccgaaagtt cgggaagcgg
     6181 cgatagcggc gattcaccaa tacggaacca gctgtaccgg ctctcgattt ttaaatggat
     6241 cgttggaatt gcatgaaact ttggaacatg aaattgccga gtttgtgggc aaagaagcgg
     6301 ctttggtttt ttcaaccgga atgcagacga atttaggaac aatcagcgca ttagtcggtc
     6361 ggaatgatta tgccattctc gataaagatt cccatgccag catttatgac ggcgtcaagc
     6421 tttcctatgg taaattggaa cgatttgcgc ataatgatat ggaacatttg gaaaaggtct
     6481 taaactctct cccggaagag gccggaaagc tcattattgt ggatggtttg ttcagtatgg
     6541 aaggagattt agctccgttg gacaaaatta ttcccatcgc aagaaaatat ggcgcccgtc
     6601 tgatggtgga cgatgctcac gggatcggtg tgacaggtaa tggtcacggt accgctgcac
     6661 aattcggctt gacgaaagag gtcgacttga tcatgtcaac tttcagtaag agtttcgctt
     6721 ctttgggcgg ttttatttcc ggcgatgaag agattgttca ttacattaag cataacgccc
     6781 gatcgatgat tttcagtgca agtattcagc cggcaagtgc tgcggcagct ttggctgcat
     6841 taagaatcat gcgagaagaa cccgaatgga atcagcgttt gataaagaat ggaaataaga
     6901 tgcggaaggg actgtccgag cttggattta atatcgggaa ttccgtaaca ccggtcgtac
     6961 cgattcttat cgggaataat gaacagactt ttgaaatggc aagcggcctt tttgaaaggg
     7021 gcgttttcgt taattcggta atttctcccg caacccctcc cggtagacaa ttgttgagaa
     7081 ccagttacat ggcaacccat acggacgaac agctcgatca ggtattggac gcttttgaat
     7141 atgtcgggaa aaaggttggc ttgatctgat gggaaaaaat tcagagaaat ccgaatatcg
     7201 ggtcttatta tttgatatag acggtgtgtt ggtttctcag ggcggatatc ggaaagcatt
     7261 tacagatacg gtttcctggt ttatgaatta ttttggattt ccggaaattc aaatcggcag
     7321 ggaatttgcg gatcgatttg atgcatacgg cgttttggcg gaatgggata tggttccgtt
     7381 gactttatcc ttttttttag attggtatgc cgagaataac ccgcaagcaa gaccttttgg
     7441 aaaacttgaa aaattaaata atcttccccg catccatccg gccatgccgt ttccggaagt
     7501 tctttggcag aagataccgc agattcaaga tattttaaat ggtgaagata tccccgcttt
     7561 agctgtttac aaacattgtc tggatcagaa agatcagagt attttaccca gactatggga
     7621 tgattcggtt ttaagtgagt ttttggcgga ttctttaaat gtgcgtactt cttggtgctt
     7681 tcagaaattg gaaacctata ttctcggttc ggagcttttt gaaaaaacat ttcatatacc
     7741 gaccgaaaag ggaattcctt ccggattaat gactgtgaat aaagcccttc ttaacgaaaa
     7801 ataccggaaa aaaattcttg aaatgaacgg aaaatcattg tacgtggcga cgattaccgc
     7861 tcgtccgaat ttgttgccgg catgtattaa agacgataaa acagagatta tctcggcaat
     7921 ccccgaagcg gaaattgcga tggatgaatt aggttgggga gatgagcgga tcaaaaatgt
     7981 cggagtaggg atattacatt attatgagaa gaagcgggat cttccgcaag acagcagatt
     8041 gaaaccgaat ccttttcatg cactttttgc gatcctttat gccatcagtg attcttttga
     8101 aaaaactttc aacgtcgttt caacctggga ctttcccgaa aaatccgaga ataaaatcaa
     8161 tccttttttg cctattcttc cggaaggttc tatgcttgat atcgcggttt ttgaagattc
     8221 tctatccggt atccaatccg caacagatgc ggcaaaaatc ttacaaaatt acgggtatat
     8281 cgcccaaacc agaagatatg gcatctatac aaatcctgaa aaggcagaat tgatgaaaac
     8341 gatcggtaca aagtgttttc cgacaatcaa tgatgctttg gatcaatatt tttcggaaac
     8401 agatcaataa aataagcatt gaattaattt ctcattcgaa tcaaatttca ttgagagtcg
     8461 ctgagcacat tgtcgaatat aagccggata atgcaaaaaa tcgcgatctg tttccgctgc
     8521 gatctgcaag aacgaatcga cggtcaatga tccgatttgt aaggctttat ttactgcctg
     8581 aatttttaaa aggctccaga caatcactac ttgggcaatt tcgaatttaa caatccggtc
     8641 gacggttatc gactcttgct gatattggga aaattgtttc aaacaatccg aaaaaaagaa
     8701 taaaaccatg aaattttccg gcaaatgagc atatttttgg gaaaaaagtt ccatcatatt
     8761 gtctcttccc atcctataat gctcagccat caatataatc ggatctgtct cgcccgcgag
     8821 caaactgatc atattccttt ggatcgctat ggatgatttc gaagctcctt ccaaccccca
     8881 gttaagacat tcatttaaaa cttttatcca ttcggaaagc cggacaggat cctcgcattc
     8941 gccggaagta aaaaacggat cggaaagctc tccggatgaa ggaccgatca aaatgttgcg
     9001 aattgcttct tcgtccatac cgttttcgat catttcaccg atggagatca ggaggttgca
     9061 agcgtttgcc agccggtccg gcatctttct tccccgattt tggacacatt taatgagtgc
     9121 tttgacgata tcgtcccgta attttaagag aattcgactt ttatcgtctc ggataaattc
     9181 gcagaatagc caatcggcat gaggatctat atatgtttcg atcgattcaa aatccatccg
     9241 attcggattc aacaacgcca attctctcac tttcggacaa gagagcgatg ccgaaagaaa
     9301 cagagaagaa tcaatttgcc aataataacg ggggaagaaa aaacaggtat cacaaagtgc
     9361 gaattcaccg tatttttcct gtaagcgaca aaattttgct tcggaaagaa aagggcaaat
     9421 ttcatcgggt tgtttcagca gattgaggaa atattcggtt gaacggttct ttcctttgcc
     9481 tgtacttatt tttcgtaaag cggaatccgt tatttcatga agatttttat ccgcgagatt
     9541 ttgatagaag cttgcatgtt tttcatcaac cgggatattc catccctgac aacaggtttg
     9601 aggacaattt tcagccaggc atttaaattc tttcatccaa gaaggagaat aacagcaact
     9661 ggtctggtaa taattttcca tgaaagcaag tgaacctttt ctattcttcg gtcgtctaaa
     9721 gaatatggat tttttccgat caggcggaac ggaaagagag atcgtgtctg atcggcgctt
     9781 gaaacaagta tagtagaata aaaacaactt gatatgaata gaaaggaata tttatgaaaa
     9841 aggttctgtt caagaaaaga ttttcggcaa tcattctggc cggtttctta atcgttacga
     9901 ttcttggttt tataccttct tccaaacaaa ttgtttggtc aacgcagacc ccccaaagcg
     9961 gatcatccgc aaccggtgaa gtaatcaaaa tgcgcaatcc ggtcattcgg tcgggtgcgg
    10021 taattatatt gaagaatccg gtcggaaccg tcgtgccaac atccgttcag aaaagcaatc
    10081 cgtcaatcca gctattacag acttccacgc cgatgccggt tatccctccg gtaatttggc
    10141 aagtcactcc gacgctcgga agggcggtag aagtgattcc tacagggatg aatgtcccga
    10201 aaccggcata tgattgcaac gttcaagtaa acagtcctta ttggtatcag caatttgcac
    10261 cgggagaaga tttcgatttt gatgttacga tcaccaatac cggtaccgaa gcatggaata
    10321 cggatatcga tgtgatgcaa tataccggat ggcgaatgga aattgaagga aagtacctct
    10381 atgatcttga taaagattac gattccgccc aaattgtttt acccggacaa tcgattcgat
    10441 ggaaaatccg aatggaagct cccaaggaga agactacaga ggacgataag tattacacca
    10501 cctacagcct tgttaaagga acggattatg ataacagcag attctgtccg ttttcccttt
    10561 atatttatgt cccgcataaa tgattttttg tctatctgat tgattaatag aaagagaaat
    10621 gcggctcccc gagagccgca tttcttatct cgctttataa ataagaaatc gaatcacatt
    10681 tcttgtctac cggataaggc gcgtaacaaa gtattttggt ctgcatattc caactcgcct
    10741 cccgatggca gccctcttgc cagacgggtg atacggacat ggtaaggttc caaacgttgg
    10801 cggatataca aagcggtggc atctccttcc atcgaaggat ttgtcgcgat aataacttct
    10861 ttataatcac ctgtcgctac acgttcgaaa agaggagcta aacgaatatc ttcgggattg
    10921 atcccttcga tcggtgacag aagtccatga aggacatgat attttccaaa gtagccgccg
    10981 gattgttcca gcacaataac atccataggg gtttccacga cacacaattc atgttgatcc
    11041 cgttggtcat tttcacatat tgcgcataaa tcgtggcctg ccaaggtgat attaaaacat
    11101 tccttgcata atgccgtctc ggtcttcaaa ttcattaatg cggtggataa tttttgcgcg
    11161 atatccgtgt tgttcttcag cagaaaaaaa gtcaatcgtg aagcggtacg agggccgatt
    11221 ccgggaagca ttgaaaaagc atcgatcaag ttttgaaccg gagcgggaat atttgccatt
    11281 tgattaaaaa cccaacccca tggaagataa tgtgttactg atcgagctca ttttctcttc
    11341 ggaaagttta cggctctgtt ccaaagccaa attaatgccg cttaaaatca tatcctgaag
    11401 catttcggca tcggcatctt tgagaaaatc cggatctatt tcaacggatt tgcagttctg
    11461 gtcgcctgtc atagtgattt taaccgcccc acctcccact gtcgcggtaa cagtggcttc
    11521 ggcgattttt gcctgttcct cttccatttg cttttgcagc tcaatcagct gctgcctcat
    11581 tgcattttga ttccccgcag gtctgccgcc cggtcttccg tatcctttag ccatttgctt
    11641 ctctcctctg atatttcgtt attttctttt attcaacttt tcaattatct gttgtatttt
    11701 acccagattt ccgaattaaa ttctttggta cttaccgatc tgtattctaa aatgaattgt
    11761 ttctgtctat tttgtttttt ctttgttgat gaccagtttc ccgcctaatt gcagtgcggc
    11821 attaaccagc ggtccctgag attcaggttg gcggtttttt ttgcccagaa ttgtcaacgc
    11881 cacaccgacc ggttttccca acacatttga gatggcggcg ctggtccata gcatgctttt
    11941 ccggctgttc tccatttgat tcttaattgt ctctcgcggg aatccgagat agagaacgcc
    12001 gtctcgaact tcgagcagct gcgcatgatt taaagtcgca tccagaatcg catcatgttt
    12061 ttttatcagt tctcggactt ttgtccaatt tttttcgatt tcttctttgg taaccgaagg
    12121 atccggttgt tgtcctttcc ggataaaaac tttggcaccg gcataatcat ccgcctcttc
    12181 cgtaagttcc tcttcatccg gcagaacgga cgctgctttt ttagccggct ggtcgtttat
    12241 aaccggcgat tggataaaaa cgggttgtac cggtttcttt tcttcgcaac tttcggctgt
    12301 tgccagttcc aaactcagcc cggggtacca accggtgcga ctgtctgtta cagcttgatc
    12361 aaacagccgg atcatttcga tcacctgatc caccgtcagt ttagcggatt gttttgccaa
    12421 aatactttga atttcagccg ttatttccaa aggctttctg gaacccattt ggataaccaa
    12481 tatcatccgc aagtgctcca ccatttggcg agcaaactgt ctggggtccg agccggaatc
    12541 caatgcctga tgaaggatgt ccaagcctaa cccggcattg tgatcaataa tcgcatctac
    12601 caattgaatc acagcttgat ttgacgacac tcccaggatc gattgtgcgg cttgcaatgt
    12661 gattttatcg ccggttgagg ataattgatc caacaatgag acggcatcgc gcattgcgcc
    12721 tgtggcttgc cgtgcaatca atgtgagagc ttcttcttca taatcaaaat tttctttttc
    12781 gcaaatgtgt ttcaaccaac cgacgatttc ggtaaccgga aacctgcgaa attcgtgatg
    12841 ttggcatcgg gaaagaaccg tggcgggaat tttctggatt tcggtggtcg ctaatataaa
    12901 catagcgtga gcgggcggtt cttccaacgt tttcaacagg gcattaaaag cggcggtgga
    12961 gagcatatgg acttcatcga taatatatac tttcatgcga ccttgagccg gcaaaaaatt
    13021 aattttgtca cgtaagtcgc ggatatcatc gacaccggta ttggaagcgg catcaatttc
    13081 gatgatatcc ataaaacttc catccagaat cgaaaggcag ttttcgcaat gatcgcaggg
    13141 acgatttgcc aaaacaggat cggtgcaatt tgccgcacga gcaagcatac gcgctatcgt
    13201 agtctttccg gtaccgcgcg ggcctgaaaa caaatatgcg tgggttaatc gatcggaagc
    13261 gattgcgtta cgaattacac ggataacctg ttcctgcccg acgaattcgt cccaagtccg
    13321 tggacgccat tttcgataca atgcctgtgt tgacaatcta accccttttt tcgctcgtac
    13381 caaacgagaa atgtttcttt ctcaattata ccgcttggat attcttgaga aagaaggatt
    13441 ggaaagtgag aatcggtctt tacaccgcga aaaacaaata tgtcccgaat ataaaagaag
    13501 tgatattaag ggattcgata agtcgcctga atattgccgt cagggtcttt taatacgacc
    13561 gtttcaccgc tttgccaagc ggcttccgta agtccccaat aaagctcgaa aggagtgtta
    13621 tgccccgagc gggaatataa agggacgatg atgccttttc gaattatttg aatattgggg
    13681 aatgtatata cgttcccatc ggcatcttcc aatgtccaat tttcaagctg gacggcggta
    13741 tcggcgttgc ttttaatgta aacggcttcc gaattaatat ccccggtgcc gattacatcg
    13801 gaaatactga ctaataaatc ccgtaccgga gggatagtag gttgacttgt aacatccgct
    13861 gtgacctgcg acgtcgctga gctgtttatt gggttattta cctggattgg cgaagtaggg
    13921 gtcatcagga tgttatagtc aatctcaatc gtcggagaag aaacgactgg cgatgcggaa
    13981 tcattaacgg taattaatgt atacggcttt tcagcgtttc gaatattttc ccaataatat
    14041 aaaaccgcga aggtcgcggt aacggataca attacattta ccaataataa tatcaatatt
    14101 ttccaaaagg tcttcatcgt ccgattatcc agataatttc tatgaaagat tttacactaa
    14161 aaacaggtcc ctaataaata ttaatagata tcgacccagc tgtcaaaaac agctcatatt
    14221 tcgaagataa aaaataacaa ataatcgaaa aaactcataa gtcgtcgcag cgggttcttt
    14281 tgagatcttt ctttttcaaa attctttatt aaaatgaaga tatggttctt tatattgacg
    14341 ggcataactt aattccaaaa attcccggaa tgtctcttcg gcaaattgac gatgaagaac
    14401 acttaattga attattacag gaatatagca gaatcagccg aaaaaaagtc gaagtatttt
    14461 ttgatcgggc agctcccggc ttcaatggtg ataaaacggc aggaacgata ttggtgcatt
    14521 atatatccga gaaaacaacc gcggatgaag cgattattcg gcgtgtccgt gcttggggga
    14581 aacgaagcaa agaaataacg gttgtttctt ccgatcatca cgtgcaaaat caggtgaaag
    14641 cactcggtgc agggatgatg acttccgatc aatttgcccg agaaattgtc tatttatcac
    14701 agcagtcgca accgaaaaat aataagaagg acgaacccca accggagcgt cctctgtcgg
    14761 aaaaagaaat ccgggaatgg atggatttgt ttaattcagg accgtcagag aaatattcaa
    14821 aatgatcaga taaataatcc agacgtttat cgggatcatc agatatccgg cgcgatgatc
    14881 cactaaccat gccattgaca gaacgatcaa accgagaata atccccgcga tcacaaggac
    14941 cagggcaccg gttaaattcc cggtgttaaa catcaagaaa ttccaacaaa atattaatcc
    15001 cagaagaatc aatgtattga tcaatacatt tttcttccga ttggggttga tttgtctctc
    15061 aagaatgaac caaacagcga ttccgaccaa tgccaatagg attatctgaa cagcaatcag
    15121 aacattttcg gaagggaaaa aagcggggag atgaatcgct ccccgataaa ttccggcata
    15181 atcttgttga atttggttga cgcagcaaaa accgaaaaaa ccggctgtta taacaagaca
    15241 aaggattaat aagggtattt ttttcattct gagacttctt tcgtttctaa atgatgatgg
    15301 ttccgttgta caaaagccaa gaacagacac atccgcatat tatagctgag ataatattat
    15361 tgttaatcga tcgtttcatc cgattctgta taatcaataa taaactaaaa atgataaaac
    15421 tgtacataac ggtatctgtc atcgcaccca aacaaagcgt gaagaaagaa tcggttgtga
    15481 cagagggcaa cccggctaca gcagaaaact gtcgggtcaa gaactccaag cccggagtgc
    15541 ctccaaataa aacagggaaa aattgaacgg cgaggaaaag cagaaaagct ccaatgggaa
    15601 tgaaattatc aaacaagaag tttttttgtt ttggccatgt tgcgtgtaac caaaaaaata
    15661 gacaaaagaa aagagagtat aggctcaggc gcgttttatt aatatccgaa aaagggcgta
    15721 agcccggaac tataaatctg aattcaatca tgaaaactct cgaaaatatc caaacgatac
    15781 catagaggat cagacatatc acaattcccc ggatgaaaga gaggaataag tcgacaaatt
    15841 ttaactgttg cttcctcaaa gtcccttttt ttgaaaagaa tccggaaacg agcattataa
    15901 acgaagcggt ccccgcccat attacgatcc cgtcagagac attcagctga ttctctgctc
    15961 tttgagaaaa tcggaggaaa atccaatatg tgataagaag taataaaaaa ccgcagaagg
    16021 agccgccgat ttgccgtgaa aaagaaaacg gtttcggatt tatatctttt tttgagtatt
    16081 ctgagggcat ttcgagaaga tgttttttgc cgatcatttg acttaatggg atgagtgtta
    16141 atagaagtgc gacgaatccg accgataatg aaatttcgcg taaggtactt aattgtttac
    16201 cggcatcaaa ccaaaggggt gagtcatttg atatttttaa tgtttgatgg aaccgattga
    16261 gtgtttggga gataaccttt gaatcaatcg gagcaaaagg atgaatggca aaaaattgct
    16321 catccggaga tgcggtttga tcgatctgat aattttgata ttcatcaaag ggtgaattaa
    16381 ttcgaagaaa atatggtttg ttttcaacat tttgataatc cgataataaa ttcggtgcgg
    16441 atgcgataaa tacaaccgat cggaaattct cttggatgaa tttttcggcc ggttgatcta
    16501 atgtcgggat cgaataagcc gcaagaccga tataatcttt caaaacgaaa gattgggatt
    16561 ctaaaaattg gaatccggct gagagaattt ccatcaagtt attccgggtt gattcgacag
    16621 aatccccctg aattgacaaa tcgatagata aagcgacaaa tccgcgtcgt gccagctcag
    16681 aagccagcga agaaagtgct tcattatccg gatttagcgc attgatcaag agaatggctg
    16741 ggcgttcatt caatgattga gcggtaatcg gtcggtagat tttcccttcc gcaaactgat
    16801 attccgtaat ctcaaccggg actttgtgaa ttaaaacagc tccccaatct gtatcgattc
    16861 ctcttgcgat cgcaccggaa gacaggacga ttgcaaacaa atacagaaat aatttagcgg
    16921 gggaattccg gacagacttc acgctatcga ttaatgatca ttgcagcgaa acactttttg
    16981 ccatctcatc cagttccgtc agagaaggtt tccaactctc cggaggaaga gaattccaat
    17041 caaaccccgc cggattaatc ggacttgcaa tttgactgtt actgatcggg atggtgatac
    17101 ttaaataata ttttccgtct tgagagattc cctgaaccgt atataacaaa ttaccattgt
    17161 caaccatcgc tccgctgtct ccgtaagcgg taataaaacg gattgccgtt cccgatatga
    17221 aatttattat ttccggcatc gccacaaata attgggtttt atcttgatat ggaaggaacg
    17281 ggatcggtgc tgtaaccgca ttgattcctt cccccccgtt aattttatta atcatggttg
    17341 tcaaatccac tgctttttga taaatttcgg aattcacatt ggataatccg ttcatcgaaa
    17401 aagcggtaac ttccggtgag aattttcctt cgactttgta atcatcgatc acaacggctg
    17461 cgctcgagat tcttgcttcg ctcaatgatt tcccttcggc tgccttttca ctattcataa
    17521 cggttaaaca acgtacattg gtggatggaa ctgacatttt tatcgtcacc gtcccgtttc
    17581 cgcattgcac ccgaggattt tcggcataaa ccgccgtcac ttctaaaaag atcaacagaa
    17641 agaaaacaat caatagattt ttcttcatgg taagctcctt tcaatttttt cacgattgcc
    17701 tattttttta caattattga aagattgttg atgataaaaa aaatttcctt atcaataata
    17761 atcgctaaaa acctttacaa aaacatttct ctgaaaattc catttttcca ctttaatgat
    17821 atcgattatt tggatttgaa cctcaaattt atttgttagt tagatccata accatataat
    17881 attctccgcg ggaaaaacct ctggattcgt aatattttcg cgttccgatt gccgaaatga
    17941 cggcgagttt cttgaagttg ttgttttttg cgatttgaat ggctttatta atgagttttt
    18001 tccctaatcc ggcatgttgt gccgcacctt tcaactccat tccaaccggt aattcctgtc
    18061 cgtaaacatg aatttcgcgg atgattgcgg cttcgttcag atcggcaata tccaaatttg
    18121 gtgaagaccg ggtaggaagt gaaagacgca gatatccggc aatcttatcc tcgggtgtca
    18181 caaattgcaa aaaatgttct tccgcttctg cggcatgata agaataatcg actaatttaa
    18241 gagtcgtcgg atccactgct tggtctctga tttctcggca acggatgcag ttgcatcggg
    18301 tcccgttttc agccattctc tggtgaatat ccattcgcag acttgaacgg gtattccctg
    18361 cgacaatatg atgagaaggg atatcacgga taattcgatt tacccggcaa tagggaggaa
    18421 taaagggttt aattccggcg atcaaatcga tcaacgtttc tgtatcatac ggaagatatt
    18481 ttcccgcctg ccatacctca tataaatccg aattctccac cagttgtgtg ggataaattt
    18541 tcagctcgtc gggacaaaat ccgagacctt ggtctgcttc aacccataat ctcataaaat
    18601 cttctcgatc agattgaggg gtacttccca aaaggttcgg catccaatgc attaccagtt
    18661 taaagccggc agcacggcaa agtttactcg cctccaatgc ctgttgaacg gtatgacctc
    18721 ggcggttcaa attgagaatt tcatcatcaa gactttgaat cccgatttga atttttgtca
    18781 ctcccaattt tcgcagatat tttaaattcg attcggtaat ttcatcgggt ctggtttcaa
    18841 cgaccaatcc gacatttcgg tgagctgccg tttcattttg ctgctgggcg atcagaagac
    18901 tttccgagtc gctttcgttc atggcgtcta aacaccgttg aacaaaccaa gcttggtaat
    18961 cttcgggata actggtccaa gaaccgccta agattaataa ttcgactttg tcggtcggat
    19021 ggccaagcgc ctgataggtg tccaaccggg atcggacctg tgcgaaagga tcgaaatggt
    19081 tctgaaatgc cctggcagct cccggttcat ccggaagata actcttgggc attcttgcgt
    19141 cattgggaca aaaaatgcag tttcccggac acgggaaggg tcgggttaat acggtcacag
    19201 tggagacgcc gctcagagtg cggttcggtt tcattcggat tttagcgagt aagtgcggat
    19261 cttcctgaat ttctccgttt gccaccattt ctcgataggc ttttaccaaa gcatctttgg
    19321 aaatatttcc gtgtttcggc acaggatttg ctcgaatcgt cttgaaaaca tccgcgccgt
    19381 caatgacccg aagcaatatt ttccgagcgg tttccataat ttccggggtt ataacttttg
    19441 atgcttccca ttccgcgaca tttttcaaat gagctttgtc attcgaaacg ggttcgtaat
    19501 caatcttatc gttcatcctg atctaattct atatgacttt ccgcaaaaat gcgattcagg
    19561 actgcggttc tttcggattc ggtcataaac cgatttaatg cgttatttgt aaatttaatc
    19621 gttaatattt ccgtactaat atgttttccg ttataaaaaa catgtctgtc cattgtagct
    19681 tcgctggttt ccggaaggaa gaatgcctga tcaaagaata aatttccgag tccgtaatgg
    19741 atgaacgagt cttttgaaat gtcgaaagcc atcggaatgt gggattgact gccgctgacg
    19801 ataaccgcac cggcatcggc gacggcttta aaatccgcgg ccatgtcctc attggggtag
    19861 attttgtaca gttcaatatg ttgaaaagtg acgatcggaa gaataccgga ggcttttagc
    19921 tctttgattt gagaaactat taagggaata tcacaatggg cagctcccgg acgagtatcc
    19981 gatgctaccg catagcctat ttccttgccg ttgcagccaa taaaagcaaa cttattccca
    20041 tggtcctcaa caattagggg cttttgtgct tcttttagat tggctcctcc gccatagtag
    20101 ggaatgtttg catctttgta taactttaat gtatagtaga ctgcatcatc accataatct
    20161 tggaaatgat ctccatccaa ttcaacgaca tctgttccaa ttgatttcag caattccaag
    20221 gctgatgttt tgctgcagaa aaccagcctt gccaattcgg attcggtttg ctgacaaact
    20281 tttgcgaacg gaacttcatt gttgacatga aggatatctg cttcgcgcag cgtatcgccg
    20341 atattcgaag ccggatattc gggaccccag acatccatat aagcagcggt accgcgaacc
    20401 aatgcggtga caccggttaa aatcattgtc gtgagtttcg atggatcgcg attgagcggt
    20461 tgaacagctg cccaatcttg ttcggtcata ttctcattcc cgttgattcg aatcgtcagc
    20521 ggccataaat tgtcgttgaa atctttatga aggggattct gctcatcaat tgatatgact
    20581 ttgaatctcg catccagctg ttcgaaaggg acgataagcc aattcccgtt atttatcgat
    20641 tgagcgaaag cggcatggat atccgtgacg aattcgatct gatttttatc cccttcgccg
    20701 aaaagtgttc taaatctttc ggggtcggat gtgacgatca gaagttttcc aggtattgtt
    20761 tccggttggg aagccgaagt cccagaccag aaatccgaga aatctcgcaa tgagatatta
    20821 tcggtaattg tggtaaacgg agctgccaga gcataaacga tttttgccgg cttttcctca
    20881 attaccgagt ttgcgaagac ttgattttgg acgattgtta atagaaaaaa aggaaggaac
    20941 aataagtaag aaaaataggt tgcttttttc atcccgctaa ttataagacc gttcaagttt
    21001 c
//
LOCUS       DAOSMM010000038        20973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2533_length_20973_cov_19.489100, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000038 DAOSMM010000000
VERSION     DAOSMM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 20973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20973
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2533_length_20973_cov_19.489100"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="PK898_06630"
     CDS             complement(<1..1859)
                     /locus_tag="PK898_06630"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOU19744.1"
                     /translation="MKREKDNKKTTNILEKIIFILFSGITLGVIALIAFSLIYQKSYA
                     DKIYKGVMISGVDIGGSTKEEADRILREKLKYPTEAAFAFKYEDRIWHAIPEELGMQI
                     QFNATVEKAWNYGRNKGSFENLQDRFYALLYGKNLEPVLLFDERVAFNFITSLAGYID
                     EPLEQPSITLQGSEVIITPGKSGRSVDRLMTMTLLQILGRNLQTAEIDIPVIQLEPTA
                     SNLAEQKQILESLLGQDFILYVKENNGNQEVDRISADLLAGWINFQPVITGNEVTIEM
                     IPKRDPFYNRLVTVSVKLYKKPQNARFMFNDQTGLIEPIAEAVVGQELDIEATLSNIT
                     KAIQSGEHEAEAVLTFTQPKVLSTANGADLGITELVQSQYTYFFGSSEERVQNIQASA
                     ASFYGLLIEPGETFSMAENIGDIDINNGYAEAPVIVGGETVQGIGGGVCQVSTTLFRA
                     AFNYGLPIVERHQHAYRVFYYERTANGNIDPSLAGLDASVYIPVLDLKFTNDTPYWIL
                     METYVNPAASSIQWKFYSTNVNRYVDWTTSGLTNVTKPDPPLYRENPELAPGEIKQVD
                     WEVDGADVEIIRTVYENGAVHLQDRFITNYEPWQAVFEYGPGTPNMPPEETKEP"
     gene            complement(1871..3262)
                     /locus_tag="PK898_06635"
     CDS             complement(1871..3262)
                     /locus_tag="PK898_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19745.1"
                     /translation="MLNGNPLLKNWNGGFFTALTGDILWAAFFRLFLSQKAVLYLIGP
                     LSYALIVLFCFLILSRNQPHHRQKYIGFFLIPILFVPKALRHPLMTLGMHGIAILYIL
                     ILTWLFGILAAENKSSLLMRTVYFFLLWMASIADGYVIYYFAIPALSVLILDAIQSRS
                     KKNRILFFLTLFGTVAGVATIQLINRMGFLRTEKTAFELIKFSDWGKYICNTADTWFR
                     IFGFSFHEFSIRKPDAIFEISGAVTALIVLILILKAIKDFFQQDMTTKVLIAGFLFCT
                     GSFTFTAVTQAEPAVRYLSPAFFSGLLIAGKQILRMPPRRKSERWLLGIPLLLFALGN
                     VSVTYYAAPVTNPSYVEIADALKDRGVKAIYATYWETHALSYYSGERIQAAPIMAEND
                     KIIPYRWSSKTDWYEPTFNATAILISQNKRYGLSEDLLIRTFGEYRERFLLDETEIYI
                     YGKNLSAIFANAE"
     gene            3523..4593
                     /gene="gmd"
                     /locus_tag="PK898_06640"
     CDS             3523..4593
                     /gene="gmd"
                     /locus_tag="PK898_06640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU19746.1"
                     /translation="MKKALITGITGQDGSYLAEFLLEKGYEVHGIVRRASTFNTRRID
                     HLYNLSQAAEDPIPLFLHYGDMSASESLLDIIYNVSPDEIYNLAAQSQVRVSFDMPEY
                     TGDTTGLGTIRILEAMRKTNSKARFYQASTSELYGKATPPQNEETPFEPQSPYAAAKL
                     YAYWITRNYRDGYGMFACNGILFNHESPRRGETFVTRKITRAVAMIMAGLQRSLYLGN
                     LDSKRDWGYAPEYVEAMWKILQQDKADDFVLGTGEAHTVREFLDEAFGYVGLDWNQYV
                     KIDSRYFRPTEVDYLLADASKAKRVLNWEPNIKFHELVRIMVDADLEALGLESPGEGR
                     KILNKKFGNWHRWEDQVISMER"
     gene            4597..7371
                     /gene="polA"
                     /locus_tag="PK898_06645"
     CDS             4597..7371
                     /gene="polA"
                     /locus_tag="PK898_06645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU19747.1"
                     /translation="MSIYLLDGHALAYRAYFALTAGSGADRWRTSKGEPVAGIYGFAS
                     ILMRLLDKEKPAHIAIAFDTGKTFRNDLYAGYKATRAKMPDDLRPQIDRIRELTDIFG
                     FPRLEMEGYEADDVLGSTAQIAVKDGYDVKIVTGDKDLLQLVTDKILVELSGNKLSET
                     KEYDVQTVKQYLGILPEQVVDYKALVGDPSDNYPGVPGIGPKTAVTLLNQYGTLDSIY
                     EHLSEIKESIAQKLKANKESAYLSRELAKIRTDLSLDINLALGATDRIDFPAVEKFFK
                     ELEFKSLIPKLRTLAPTFQPIQDQLSLFGLSNADDEQPESAHEARCHIIDSEQDLIRM
                     IEKISQANSLSLDTETTGTDPMDCHLVGISLSVETGMGYYIPVGHLTGEPQLDLEKVR
                     QSLNPLFLREDIVKVGHNIKYDLIVLRNHGFEVKGKLFDTMVAGWVIDPESKNLGLKA
                     MAFDLLGINMQSIDTLIGKGSNQLTMANVSIKDAAPYAAADADMTLRLVPILKETLKK
                     RETEKIFDELEMPLIPVLTDMEMAGIRVDRDFLKEMSKSLEIRLSEIEKDIYAGVGYE
                     FNINSTQQLSRAIFETLKLEPPGRKKKTTSGLYSTSADVLEEMRGSHPIIDLILEHRE
                     LSKLKNTYVDALPLTINRRTGRVHTSFNQTGAVTGRLASSSPNLQNIPTRTELGRKVR
                     TAFIADPDHVLLSVDYSQIELRIVAHMAQDEGMLAAFRAGQDIHATTAAAIYGCDISQ
                     VTKEMRRHAKAINFGLIYGMSPYGLSQSANLSRKEAEEFVDAYFKKFPRIKEYLDSIK
                     IQAAEKGYVETMMGRKRYFPNLGQQKNYSLRTREEREAINAPIQGTAADIMKLAMIQV
                     HDKLKKSVLQAQILLQVHDELLLECSQKDLDETRELVQDTMENVVTLSIPLLTEARSG
                     LNWGDLTPMP"
     gene            7418..8047
                     /locus_tag="PK898_06650"
     CDS             7418..8047
                     /locus_tag="PK898_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU19748.1"
                     /translation="METDESKLIKGAIQGNLNAFNQLVMIYQTAVYNTALRIVYDEDR
                     ANDLAQTAFISAWQHMDSFKGDSFKPWILRITINACYDELRRQRRHPSVPLEPVSTED
                     DEENEDVHWLMDPSPQPSQTTETNEMIQAVENCLNRLPFDYRSVLLLIDVQELDYQEA
                     SEVLDKPLGTVKSRLFRARQKMRDCLKEKGELFSSEDRYFDRKDEQDNG"
     gene            8028..8816
                     /locus_tag="PK898_06655"
     CDS             8028..8816
                     /locus_tag="PK898_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19749.1"
                     /translation="MSKTMDNRKAKDLELINTYLDHEMNDQDLLDFAERLSQEENLKE
                     DLADIMQVRSQLRKLPMKKPPQNYILTRSMAQEVRKPNLLERFFPIFRTAAVFACIAF
                     VLTFILPLSSISSSTKTEPMLAKKSVEIDLIDKSDEANSEKVVTPEVFSEESVNPLAD
                     SELMVQAFPSKGVRGGNPKLEYLTNAERMIPDDRSPVSTPEISSQPISGEAAQKEIQN
                     LINGVSESQTTGSAETIIRLVSIGILAVSLFWIAITLIKRRQWA"
     gene            complement(8842..9762)
                     /locus_tag="PK898_06660"
     CDS             complement(8842..9762)
                     /locus_tag="PK898_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU19750.1"
                     /translation="MEQNTGKPFSQHSAAEKRKIILLFLVCIFLWASAFTAITIGARE
                     FEPGHLALLRYGFASLILIVYALLKKMPLPRLQDVPIFLLTGFLSFTLYNITLITGQK
                     TVPAGTSSVIIALNPVIVALLAGLVLNEKLNRNGIIGIFIAFIGVVIAVIDWKHGLTL
                     TKGVFWILIASLSASTSTILQKKWIARYSGEQVMTYSIWGGTLLLLFYLPGLVHSLPS
                     ISTKAILTALYLGAFPGVIAYLVWGYLLSEIPAHQANSFLFMDTIGAMLIAWLVLGEV
                     PGIQVLFGFILILIGLFLVNGSRQSEKVVS"
     gene            10158..12320
                     /gene="glgP"
                     /locus_tag="PK898_06665"
     CDS             10158..12320
                     /gene="glgP"
                     /locus_tag="PK898_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOU19751.1"
                     /translation="MSINIKRVIPIASSLPKRIERLNDLAYNLWWSWNPEAINVFSKI
                     HPVLWDSVSHNPVAFLQQVSRTLLNRVANDKQYLDLYDRVVNNFDSYMGEKETWFVKN
                     YPEVTAGQSIAYFSFEFGLHESIPAYAGGLGILAGDHLKESSDMGIPLEAIGFIYNQG
                     YFVQKITEDGWQETSNFYLDFNKLPMIPILDEKDKPILISVQLPGREIKARIWQIQVG
                     RVPLYLLDTNISENSSFDQQLTAKLYTSDLETRISQEILLGMGGVRVMRALGYNPTVW
                     HMNEGHSAFLSVERCAELVRQGKTFEEAKEIVRKGNVFTTHTPVPAGNDKFPVWLVEK
                     YFSQIWPDLGLTREQFIDLAKEASPYGDQFTMPILALRLSERCNAVSELHGQVSRNMW
                     HFLWPDRKVEEVPITHITNGVHTFSWLSRRMGILFDEYLGTEWRLDPSNPKVWEKVDS
                     IPDAELWAVKRHQKRKLMRFINDKARAQWLTGEVHPVQTIAAGVLLDADALTIGFARR
                     FPTYKRGYLILRDYERLLRIINNVDAPVQIIFSGKAHPADEPGKLIVQQLYRAIKDHR
                     TGGRMVFVENYDMDIARHLVQGVDVWLNTPRRPMEASGTSGMKAAMNGCLNFSVLDGW
                     WHEGYNGKNGWAIGDDKTYESDEKQDEMDALSLYETLEDQIVPIYYSNRSENGVPTTW
                     MRWVKEAIRTLSPQFSTRRMVGDYVREMYIPAIKESEE"
     gene            12481..13011
                     /locus_tag="PK898_06670"
     CDS             12481..13011
                     /locus_tag="PK898_06670"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOU19752.1"
                     /translation="MDFTVSKDLIIGGLAIFLLRICDTSMDTLRVLFVVRGKKSIVWV
                     LGVFQSTIFIIAISSALKGDNNLFTILGYATGFATGNVIGMAIEDHLAIGFKRISIVT
                     RDKGEEIAKAIRDAGYGVTEIFGRGRDGVVTMINVNAKRKHVKDIENIALAVDETSFI
                     TVDDFSPVNHSGFWRK"
     gene            complement(13349..14335)
                     /locus_tag="PK898_06675"
     CDS             complement(13349..14335)
                     /locus_tag="PK898_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19753.1"
                     /translation="MRINKLTDKLLVLFGIGCIFVFLFASCSILTGLQEVDPQSATLT
                     ALVTPTSVQPTLDLKGNKVLEDRSCLIFDSPSISTFADAEDGGIFAWAGNKNLLAFVV
                     PENRYWAWFSGDAEIVDFEKDYKNPTVMNTTGLKVFGDFAFNPSATKIAFVVLRTSEK
                     IYTVVVATLSSNLQTTIDLFTDTSARTDNYSSSKSVIQWENDDEVTVTTSCGIDCDQI
                     YRINTVTGTTRLIDEVRKKGHTGRDFPQNVPEYDEREYPAMNQPNWSPDLSMVFYTDD
                     QNKTWILNDKTKEQFELPITADKVLQTAWSHDAQFIAIRFAENLSVYKVNCK"
     gene            complement(14385..15047)
                     /locus_tag="PK898_06680"
     CDS             complement(14385..15047)
                     /locus_tag="PK898_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU19754.1"
                     /translation="MTIKILALSDVETGLIYSERSDEYFPAVDLIVSCGDLPMSYLDY
                     AATTLNAPLYFVLGNHNSLIPVSKTSNYPYNSSGYNLHCSNYFYEKRLLIAGVEGSVK
                     YNNGPQQYSQSEMWTNVFSLVPALLRNKLLHGRYLDLFVSHAPPWQIHDQQDLPHNGI
                     RAFRWLDKVFKPKIHLHGHIHVYRSDTVRETVYYNTKVINCYGYREIFFDEPTDNLPK
                     ES"
     gene            complement(15097..15468)
                     /gene="acpS"
                     /locus_tag="PK898_06685"
     CDS             complement(15097..15468)
                     /gene="acpS"
                     /locus_tag="PK898_06685"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOU19755.1"
                     /translation="MTTILRSGIDLIEIERIEKLKPEIKARFLQRVYTSAELDLCGDR
                     DERLAGRFACKEAVSKALGTGIGTVHWQEIEILTNDQQMPILYLYGNAAALAKSLGLS
                     IWSVSISHVKNYAVAIAIASD"
     gene            complement(15465..16754)
                     /locus_tag="PK898_06690"
     CDS             complement(15465..16754)
                     /locus_tag="PK898_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU19756.1"
                     /translation="MKNIQSIPGSDDILRIELPNQIVLLARENSLSPSVVISGYLPGG
                     SVLDPVDRSGLAIFLSLALMRGTETNSFEQIFRKLEDNGASLGFNAGFHSFSFAGQSL
                     AEDLPAMLDLLNDILRHPIFPENHLTQIRDQMLTALAIRSQDTAEMANVAFDQALFGS
                     HPYGRDKLGTPESVQSVSRSDLVRHHAQYFKPQGMVISIVGGIEPEKAADLVRKYFSD
                     WEAGPQAIPNLPDFPKVTKPEKGSATHIEIPEKSQLDLVIGSIAPERKSPQYQPARLG
                     NCILGEFGMMGRIGRVVRDENGLAYYAGSDVNCLPLGGSWEISAGINPENLDRTVDLI
                     RSEIQKFVSTPVEPNELSDVQSYYLGRMPLQLESNAGIANELLSIERYQLGLDYLRRY
                     GDIIRAVTREQILETAATYLDPDRLTLATAGTLRGKA"
     gene            complement(16751..18046)
                     /locus_tag="PK898_06695"
     CDS             complement(16751..18046)
                     /locus_tag="PK898_06695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU19757.1"
                     /translation="MSDEIVQTKLENGLTVLLKEIHSAPIISHWIWYRVGSRNEVPGK
                     TGISHWVEHMQFKGTAKYPAGVLDQAISRGGGIWNAFTYLDWTAFFSTMPKDKIALPI
                     DLEADRMQNSLFLPEEVESERTVILSEREGSENEPLFRLNEAIDKAAFDRHPYRFEII
                     GEKSDLLTLTRDDLYTHYRQFYAPGNAVLTMAGDFDANEMLDRLAAVYQSVSARPIPS
                     VTIQPEKAIPEERHVELSGPGDTTYLRIVWRAPAGRDPDIFPLAVLDSILTGPSSLNM
                     FGSGSISNKTSRLYQPLIDRQIAVAFSGDYNATIDPYLYMMTVIMHPDHDPQEAVRII
                     DEEISRIQSGDISEQELKKAVKQARALFAYGSENITNQAYWMGYTEMFASYSWFTEFV
                     SSLNKVTVSDVAKSASTYLNSAHRVIGIYRPYPEESEES"
     gene            complement(18194..19168)
                     /locus_tag="PK898_06700"
     CDS             complement(18194..19168)
                     /locus_tag="PK898_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19758.1"
                     /translation="MMKNKKQLFVLMILVAMFAIGIATVQAEGIEPYPSYTITSVTVD
                     QSVSILTLNFPADTEFIVRMKDLNSVSDYIDVAKFNTQSGGSFTATFPIPAELVGKKS
                     IGMLLSNNNGIEIPSTFSNEAMTETVATVCDYSVLPSFTIDSVVKGKSITVTTGTFPA
                     NQSFTVKMGYYAAGAPAAHHHCGGHECPTSDPWDPWMDVDGTPEPDMYPIPDPSFSWM
                     FGPMVPGCGTSGCVNFHLGGATPHAGLIPTYFVGFEVGTYDSADGSPQTVSYDIPEEL
                     KGYSPIIVRFEDKGSCGFYGYNYFWNADFPVTSTDSVPEVQIEAVSQQ"
     gene            complement(19320..20216)
                     /locus_tag="PK898_06705"
     CDS             complement(19320..20216)
                     /locus_tag="PK898_06705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU19759.1"
                     /translation="MNLSKKRTMIFIIITFVITWAAEFMLWKGGGLKNPRVQIYLSII
                     MMIPMIGSFLTRWITKEGLENLWLRLDFKANIKTYLIAWFGPALLILFGSALYYLIFP
                     AKFDPEMSVLRQSLNALGEQQTAELTTQKLLLSILSQVLLALIMSPILNAIPCLGEEI
                     GWRAYLLPKLADFLPRKKAVVISGVVWGIWHAPIIAMGYNYGENYPLFPWLGIIGMIL
                     FCVFVGSFLAWLTFRMESALPAAIAHGAVNGMASVGIFFLRGTPEPFLGPYPVGIVGG
                     FGFIVIGILCYLNIEKAKPLQS"
     gene            complement(20264..20800)
                     /locus_tag="PK898_06710"
     CDS             complement(20264..20800)
                     /locus_tag="PK898_06710"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOU19760.1"
                     /translation="MQPSLTHGLNILYTGNGKGKTTAAMGLLFRAWGNNMSVAGIQFI
                     KSPEIVKGEMIACRRLGIPFETLGKGFVFEKFDTTIHAEAARSAWDFTREVILHRNLD
                     MLLLDEITYLFVYKWLDCNEFIQWIRENKPPTMHLILTGRNAPQELIDFADLVTEMKE
                     IKHPYTTQRIPAQIGIEY"
BASE COUNT         5937 a         4579 c         4774 g         5683 t
ORIGIN      
        1 ggctccttgg tttcttccgg cggcatattg ggagttccgg gaccgtattc gaaaaccgct
       61 tgccatggtt cgtaatttgt aataaagcga tcctgcaaat ggacagctcc gttttcgtaa
      121 acggtgcgaa tgatttcgac atccgcgccg tccacttccc aatccacttg tttgatttca
      181 ccgggagcca attccgggtt ttctcgataa agaggaggat cgggtttggt cacatttgtc
      241 agaccgctgg ttgtccagtc cacatagcga ttgacattgg tactgtaaaa tttccattga
      301 atactggatg cggcagggtt gacgtaagtt tccatcaaaa tccaataagg cgtgtcattg
      361 gtgaatttca aatcgagcac cgggatatag accgaagcat ccaacccggc aagtgaggga
      421 tcgatgttgc cattagcggt tctttcataa tagaaaaccc gataagcatg ttgatgtcgt
      481 tcgacaatcg gcaatccgta attgaaagcg gcacggaaca aagtggtact cacttggcaa
      541 acaccgcctc cgattccttg gacagtttct ccgcccacaa taaccggtgc ttcggcataa
      601 ccgttattta tatcgatatc gccgatattc tccgccatcg aaaaagtttc accgggttca
      661 atcagtaaac cataaaaact ggccgcactt gcttggatgt tttgcaccct ttcttccgaa
      721 gagccgaaaa aataggtata ctgcgactga accaattcgg tgatccccaa atccgccccg
      781 tttgccgtac tcagaacttt cggttgagtg aaggttaaaa ccgcctccgc ttcgtgttcc
      841 ccggattgaa ttgcttttgt gatattgctc aaggtcgctt cgatatcaag ttcctgaccg
      901 acgaccgctt cggcaatcgg ctcaataaga ccggtttgat cattgaacat gaaacgcgca
      961 ttttgcggct ttttatataa tttcacactg accgttacca aacggttata aaagggatcc
     1021 cgtttgggaa tcatttcaat ggttacttca tttcccgtga taaccggttg aaaattaatc
     1081 cagccggcaa gtaagtccgc agaaattctg tctacctctt gatttccatt gttttctttg
     1141 acgtaaagga taaaatcctg tcccaaaaga gattcaagaa tttgcttttg ctctgccaga
     1201 ttggacgcgg tcggttccag ttgaatcacg gggatatcga tctcagctgt ctgcagattt
     1261 ctccccaaaa tctgcaataa cgtcatcgtc attaatcggt cgacggaacg cccgcttttc
     1321 ccgggagtga tgatgacttc cgaaccctgt aatgtgatgg aaggctgttc caagggctca
     1381 tcgatatatc ctgctaagga agtgatgaag ttgaatgcaa cccgttcgtc aaatagcagc
     1441 accggctcca aattcttgcc ataaagcaaa gcgtagaatc gatcttgaag attttcgaag
     1501 ctccctttat tccttccgta attccaagcc ttctcgacgg tagcgttaaa ttggatctgc
     1561 attcccaatt cttcgggaat tgcatgccag attcgatctt catacttgaa tgcaaatgcg
     1621 gcttccgtcg gatattttaa tttttcccgt aaaatgcggt cggcttcttc cttggtggat
     1681 ccgccgatat ccacaccgga aatcatgacg cctttataaa tcttatccgc gtatgatttt
     1741 tgataaatta acgaaaaagc gatcaaggcg atgacgccga gagtaattcc gctaaaaagg
     1801 atgaaaatga ttttttcaag aatatttgtt gtttttttat tgtctttttc ccgcttcata
     1861 ccttgttatt ctattctgca tttgcaaaaa tcgcagaaag attcttacca taaatataaa
     1921 tttcggtttc atccaaaagg aagcgctcgc gatattcccc gaaagtccgg atcagtaaat
     1981 cttcggagag tccataccgt ttattttgac tgatcaatat ggcggtcgca ttgaaagtcg
     2041 gctcgtacca atctgtctta gatgaccacc gataaggaat aatcttatca ttttcagcca
     2101 taatcggagc ggcttgaatt ctctcacccg aataataaga aagagcgtgg gtttcccagt
     2161 aagtggcata gattgctttt acaccgcgat ctttaagcgc atccgcaatt tcgacatagg
     2221 acgggttcgt gaccggtgct gcataatagg taacggatac attccccaat gcaaataaca
     2281 gaagaggaat tccgagcaac catcgttctg atttccttcg cggcggcatc cgcaaaattt
     2341 gttttccggc aatcaaaagt ccgctgaaga aagccgggct caggtatcgg acagccggtt
     2401 ccgcctgcgt aacggcggtg aaagtaaaac tgccggtaca gaaaagaaag ccggcaatca
     2461 gaacttttgt tgtcatatcc tgttgaaaga aatctttaat cgctttgaga atcaaaatca
     2521 aaacaatcag agcagtaaca gctccgctta tctcaaaaat agcgtccggt ttgcggattg
     2581 aaaattcatg aaaagaaaaa ccgaaaattc tgaaccaagt atctgccgta ttgcaaatat
     2641 attttcccca atcggagaat tttatcaact caaaagctgt tttttctgtc cggagaaagc
     2701 ccatacggtt aatcaattga atggtggcga cgcccgcgac ggttccgaaa agagtgagga
     2761 agaataaaat ccgattcttt tttgaccggg attgaatcgc gtccaaaata agcaccgaga
     2821 gtgcgggaat cgcaaagtaa taaatcacat atccgtccgc aatcgaagcc atccagagca
     2881 ggaaaaaata aacggttctc atgaggaggg atgatttgtt ttcggctgcc agaatgccga
     2941 acagccatgt taatatcaat atataaagaa tcgcgattcc atgcatcccc aaagtcatta
     3001 aaggatggcg gagtgccttc ggaacaaaca aaatgggtat cagaaaaaat ccaatgtact
     3061 tttgccggtg gtgaggctga tttcgactta agatcaagaa gcaaaacaaa acgatcaacg
     3121 cataagaaag aggcccgatc agataaagca ccgctttttg gctcagaaaa agtcggaaga
     3181 aggcagccca taagatatcg ccggtcaatg cggtgaagaa gccgccgttc cagttcttta
     3241 ataaaggatt tccgtttaac atgtcttcgg ctgcatgaag aaaatatacg ttatctgaat
     3301 tgaccgggaa tgaaaaacta taaaaataaa tataatatcc gattaataaa cataaaaagg
     3361 tcaaaccgga aatcaaaatg agctttttcg tttgaaaaaa ttctttcatg gatctaagta
     3421 tattcgatcc ggttcattcc gttttcgaac agcgacaggc aactatgtct gaccgattga
     3481 aatatccgct ataattcaaa aaggggacag ggagggtaat caatgaaaaa agcacttatt
     3541 acgggaataa cagggcagga cggttcctat ttagccgaat tcctgttgga aaaagggtat
     3601 gaagtacatg ggatcgtccg cagagcaagt acttttaata cacgccggat cgaccatctt
     3661 tataacctgt cgcaggctgc cgaggatccg attccgcttt tcttacacta cggcgatatg
     3721 tcagcctccg aatctttatt agatattatt tacaatgtat caccggatga aatatacaat
     3781 ttggcggcac aaagccaagt tcgcgtcagt tttgatatgc ccgaatatac gggggataca
     3841 acgggtctcg ggacgattcg cattctcgaa gccatgcgga aaacaaactc caaagcacgt
     3901 ttttaccaag catccaccag tgaactttac ggtaaagcga ctcctccgca aaacgaagaa
     3961 acgccgtttg agccgcaaag tccttatgcg gcagccaaac tttatgctta ttggatcact
     4021 cgcaattatc gggacggtta cgggatgttc gcctgtaacg gtattctctt caatcatgaa
     4081 tctccacgca gaggagaaac ctttgtgact cgtaaaataa cccgggcagt tgccatgatt
     4141 atggccggtt tgcagcgatc actttatctc ggaaatttgg attcgaaacg tgattgggga
     4201 tatgctccgg agtatgtcga agcaatgtgg aagattttgc agcaggataa ggccgatgac
     4261 tttgttttag ggaccggtga ggcgcacaca gtacgtgagt ttctggatga agctttcggt
     4321 tatgtcgggt tggattggaa tcaatatgtg aaaatcgatt cccgctattt ccgcccgact
     4381 gaagtggact atttgttggc ggatgcatcc aaagcgaaac gagttctgaa ctgggaaccg
     4441 aatattaaat ttcacgaatt ggtcagaatt atggtggatg cggatttgga agcattggga
     4501 ttggaatctc ccggtgaagg aagaaaaatt ctcaataaga aattcggaaa ctggcaccgt
     4561 tgggaagacc aggtgatcag tatggaacgc tgagccatga gcatatatct tttggatggc
     4621 catgcgcttg cttaccgggc ttatttcgca ttaactgccg gatcaggggc ggaccgttgg
     4681 agaaccagta aaggggaacc ggtggcggga atctacggat ttgccagtat cctgatgcgg
     4741 ttgctggata aggaaaaacc ggctcatatt gccattgcct ttgatacggg aaaaactttc
     4801 cgaaacgatt tatatgcggg atataaagcg acccgggcaa aaatgccgga tgatttgcgt
     4861 ccgcaaattg accgtattcg ggaattaacg gatatttttg ggtttcctcg gctcgaaatg
     4921 gaaggttacg aagccgacga tgtgctcggg agtacagcac aaatcgcggt taaagacggc
     4981 tacgatgtaa aaatcgtcac cggcgataaa gatttgctgc aattggtaac ggataaaatt
     5041 ctggtggaat tatccgggaa caaactttcc gaaacaaaag aatatgatgt gcagacggta
     5101 aaacagtatt tgggaatttt gccggaacaa gttgtcgact ataaagccct tgtcggcgat
     5161 ccgtcggaca attatccggg cgtacccggg atcggtccaa aaacagcggt aactttgctg
     5221 aatcaatacg gaacattgga ttcgatttat gaacatttat ccgaaatcaa agaatcgatc
     5281 gctcaaaagc tgaaagcaaa taaagaaagt gcctatttaa gccgagaatt ggcaaaaatc
     5341 cggacggatt tatcattgga tattaatctg gcattggggg caacggatcg gattgatttt
     5401 cctgcggttg aaaagttctt taaagaactc gaattcaaat cgctgattcc gaaattaaga
     5461 acgcttgctc ccacttttca accgatacaa gaccaattat ccttgttcgg actttcgaat
     5521 gcagacgatg aacaacccga atctgcccat gaagcccgtt gccatatcat cgattcggag
     5581 caggatttga tccgaatgat tgaaaaaatc tcacaggcaa attccttgag tttggatacc
     5641 gaaacgacgg ggaccgatcc gatggattgc catttggtgg ggatttcgct ttccgtcgaa
     5701 acagggatgg gatattacat tccggtggga catctcacgg gagaaccgca actcgacttg
     5761 gaaaaggttc gacaatcttt gaatccattg ttcctgcggg aagacattgt caaagtcggt
     5821 cataacatta aatatgatct gattgtttta cgaaatcatg gcttcgaagt caagggaaag
     5881 ctgtttgata ccatggtagc cggatgggta attgatcccg aatcaaaaaa tctcggctta
     5941 aaagccatgg catttgattt gctcgggatc aatatgcaat cgatcgacac gttgatcgga
     6001 aagggcagca atcagctgac aatggcaaac gtatcgatta aagatgcggc tccctatgcc
     6061 gcggctgatg ccgatatgac tctgcggttg gtaccgatct tgaaagagac cttgaaaaaa
     6121 cgcgaaacgg aaaagatttt tgatgaattg gagatgccgc tgattccggt attgacggat
     6181 atggaaatgg ccggtattcg ggtggatcgt gactttttaa aggaaatgtc caaatcactc
     6241 gaaattcggc tttcggaaat cgaaaaagat atttatgccg gcgtcggcta cgaattcaac
     6301 attaattcaa ctcaacaact gtcgagagcc atttttgaaa ctctcaaatt ggaaccgccc
     6361 ggtcgcaaaa agaaaactac ttcagggtta tattccactt ccgccgatgt tttagaagag
     6421 atgcggggct ctcatcccat cattgatttg attttggaac atcgtgagct ttctaaatta
     6481 aagaacacct atgttgatgc gcttcctctc acaatcaatc gcagaacagg acgggttcat
     6541 acttctttca atcaaaccgg cgcagtgacc ggacgtctgg catcttcttc acctaatttg
     6601 cagaatattc cgactcgtac cgaattgggc aggaaagttc ggacagcctt catcgccgat
     6661 cccgatcatg ttttactttc ggtggattac agccagatcg aattacggat tgtggctcat
     6721 atggctcagg atgaaggaat gttggcggca tttcgagccg gacaagacat tcatgcgacg
     6781 actgccgcag caatttacgg ttgcgatatt tcccaggtta ccaaagaaat gcgtcgacat
     6841 gccaaagcca ttaatttcgg actgatctat ggcatgagtc cctatggctt aagtcaatcg
     6901 gcgaaccttt cccggaaaga ggcggaagag tttgtagatg cttactttaa aaaattcccc
     6961 cgaattaaag aatatttgga ttccattaaa atccaagccg ctgagaaagg ttatgttgaa
     7021 accatgatgg gaagaaaacg atacttcccc aatctcggac agcaaaaaaa ttacagttta
     7081 cgcactcgtg aagagcggga agccatcaat gcaccgattc agggaaccgc cgccgatatt
     7141 atgaaattag ccatgattca agtgcatgac aaactgaaaa aatccgtctt gcaagcccaa
     7201 attttgctgc aagttcatga cgagttattg cttgaatgtt ctcaaaagga cttggatgaa
     7261 acgcgagaat tggttcaaga cacaatggaa aatgttgtga cactgtccat cccgttattg
     7321 actgaagcgc gcagcggctt aaattggggg gatctgacac cgatgccctg atcgataaac
     7381 gcaaggtgtt ctatgcgctt tacagatgaa agaaaaaatg gaaacggacg aatcgaaact
     7441 gattaaagga gccattcaag gaaatttgaa tgcttttaat cagttagtga tgatctatca
     7501 gacagctgtt tataacaccg ctctccgtat cgtttatgat gaagaccgtg ccaatgatct
     7561 ggcacaaacc gcttttatct cagcctggca acatatggac tcttttaaag gtgattcttt
     7621 taagccgtgg attttacgaa tcacaatcaa tgcttgttat gatgagctcc gccgccaacg
     7681 ccggcatccg tctgtccctt tggaaccggt ttcgaccgaa gatgacgaag aaaatgaaga
     7741 tgttcattgg ttgatggatc cttctcctca gccttctcag acaacggaaa caaatgaaat
     7801 gattcaagct gttgaaaact gtttgaaccg cttacctttt gattatcgct ccgtattgtt
     7861 attgatcgat gttcaagaat tggactacca ggaagcgtcc gaagtgttgg acaaaccgtt
     7921 gggcacggtt aaaagtcgcc tgttccgcgc ccgtcaaaag atgagggatt gccttaaaga
     7981 aaaaggagaa cttttctctt ccgaagatcg ttattttgat aggaaagatg agcaagacaa
     8041 tggataatcg gaaagcaaaa gatctggaac tgatcaatac ttatcttgat catgagatga
     8101 acgatcagga tttattggat tttgccgaac gactttcaca ggaagaaaat ctcaaggaag
     8161 atttggcaga tatcatgcag gttcgcagtc aactgcggaa attgccgatg aaaaagcccc
     8221 cgcaaaacta tattttgaca cgcagtatgg cacaggaagt tcgaaagcca aatttactcg
     8281 aacgtttctt cccgatattt cgaacggcag ctgtttttgc ttgtattgca ttcgtattga
     8341 cttttattct tccattgtct tcgatctcct catcgacaaa aaccgaaccg atgcttgcga
     8401 aaaaatcagt cgaaatcgat cttattgata aaagtgatga agcgaattcg gaaaaagtcg
     8461 ttacacccga agtattcagc gaagaatccg ttaatccgtt agccgattcc gagttaatgg
     8521 ttcaagcatt tccttcgaaa ggggtaaggg gcggaaatcc gaagttggaa tacttgacca
     8581 atgcggaacg gatgatcccg gatgatcggt caccggtatc gacaccggaa ataagctcac
     8641 agccgatttc cggagaagcg gcacaaaaag aaattcaaaa tttaataaat ggtgtatccg
     8701 aatcacagac gaccggatca gcggaaacaa tcattcgttt ggtttcgatc ggtattttgg
     8761 ctgtcagcct gttttggatt gccattacac tgataaaaag aaggcagtgg gcataaaata
     8821 atccgtttcg cagatacttg tttaagagac gactttctcc gactgtcggg atccattaac
     8881 cagaaaaagc ccgatcaata tcaagataaa cccgaacaat acttgaatac ccggaacttc
     8941 gcctaaaaca agccaagcga ttaacattgc acctattgta tccataaaga gaaaactgtt
     9001 tgcctggtgc gcgggaattt ccgataaaag gtatccccat accaaataag cgatcacgcc
     9061 ggggaaagcg cccagatata acgccgtcaa aatcgctttg gtcgaaatac tcggaagcga
     9121 atgaaccaat ccgggtaaat agaaaagcaa taaaagcgtt ccgccccaga tagaatatgt
     9181 cataacctgt tctccactgt aacgagcaat ccattttttt tgcaggatgg tactggttga
     9241 agcggaaagg gatgcaatta atatccaaaa tacccctttt gtgagggtta atccgtgttt
     9301 ccaatcgata acggcaatga ccaccccgat aaacgcgatg aatattccga tgattccgtt
     9361 tcgattcagt ttctcattca gaaccagacc ggctaacagg gcgactataa ccgggttcaa
     9421 ggcgataatc acactggatg ttcccgccgg taccgttttc tgaccggtta tcagggttat
     9481 attgtataaa gtaaaactca aaaatccggt caaaagaaag atcggaacat cttgcaatct
     9541 tgggagaggc atttttttca acaatgcata aacaatcagg atgagagaag cgaagccgta
     9601 acgaagaaga gccaggtgcc ccggctcgaa ttcacgcgct ccgatggtta tcgcggtgaa
     9661 agcgcttgcc cataagaaaa tgcaaaccaa aaaaagtaaa ataatctttc ttttttcggc
     9721 agccgaatgc tgactgaaag gctttccggt gttttgttcc aaaagagatc tcctcagata
     9781 tgtgctgaat tcgttgagag caaattataa atcaaccttt tgaatttgcc ggagtgttct
     9841 gctgtttata ggatttttta tctaaccgct ccttatgcga ttccggaata gttgttgcct
     9901 tgaattattc gggaaattgt caccggaccg gttttcatcc gatctgtaaa aacagaaatt
     9961 tcgcaattag agagtaaaat taccgaaaac agatgattaa tggtccttta ttgatgcaat
    10021 atcggtaaca aatccgatcg gttgattaat tttgtcattt tgaaatcgtg tatcattatc
    10081 tgcgggttgt cctttattaa ttcggcattg agtgctgaac ggacattccg ttaaataatt
    10141 gaatagggaa aataataatg tctataaata taaaaagagt cataccgatt gcaagcagcc
    10201 ttccgaagag aatcgaacgt ttaaatgatt tagcttataa cctgtggtgg tcgtggaatc
    10261 cggaagcaat aaacgttttc agcaaaattc atccggtttt atgggattcc gtctcacata
    10321 atcctgtggc atttctgcag caggtttccc gaaccttatt gaatcgagtc gccaatgaca
    10381 agcaatatct cgatctttat gatcgcgtag tgaacaattt tgattcctac atgggcgaaa
    10441 aagaaacatg gtttgtaaaa aactacccgg aagtgactgc cggtcaatcc atcgcttatt
    10501 tttcattcga attcggttta catgaatcca ttccggctta tgccggtggt ttgggaattt
    10561 tggccggtga tcatctgaaa gaatccagtg atatggggat ccctttggaa gcgatcggat
    10621 ttatttataa ccagggttat tttgtccaaa aaatcaccga ggatggttgg caggaaacat
    10681 cgaattttta tcttgatttt aataagctgc cgatgatccc gattttggat gaaaaagata
    10741 aaccgattct gatatccgtt cagcttccgg ggcgtgaaat aaaagcacgg atttggcaga
    10801 ttcaagtcgg ccgggtccct ctttatttgt tggataccaa tatttccgag aattcatcgt
    10861 ttgatcaaca attgaccgcg aaattatata cttcggatct tgagacacgg atttctcagg
    10921 aaattctgct cggaatgggc ggcgtgcgag taatgcgcgc attaggttat aacccgaccg
    10981 tttggcacat gaacgaaggt cattccgcat tcttatccgt tgaaaggtgt gcggaattag
    11041 tacgtcaagg gaagactttt gaagaagcta aagaaatcgt acgcaaaggg aacgttttta
    11101 cgacccatac cccggttccc gcgggaaatg acaaattccc ggtttggttg gtagagaaat
    11161 atttcagtca gatttggccc gatttgggtt tgactcgcga gcaattcatt gacttggcaa
    11221 aagaagccag cccttacgga gatcaattta ccatgccgat tcttgcgctg agactttcgg
    11281 agcggtgcaa tgcggtgtcg gaattacacg gtcaggtatc gcgcaatatg tggcattttc
    11341 tgtggccgga tcgaaaggtg gaagaggttc cgattacgca tatcacgaac ggcgttcata
    11401 ccttcagttg gctttcacgc cgaatgggta ttttatttga tgagtatctc ggtaccgaat
    11461 ggcgactcga tccttccaat ccgaaggttt gggaaaaagt ggattccatt ccggatgccg
    11521 aactctgggc ggttaaacgt catcagaagc gtaagctgat gcgctttatc aatgataaag
    11581 cgcgagcgca atggctgacc ggtgaagttc atccggtaca aaccattgcg gccggggtat
    11641 tacttgatgc ggatgcgttg acaatcggtt ttgccagacg atttccgacc tataagcggg
    11701 gttatcttat cctgcgggat tatgaaagac ttttacggat tatcaataat gtagatgctc
    11761 cggttcagat tatcttctcc gggaaagctc atccggcgga tgagcccgga aaactgatcg
    11821 ttcaacaact ttatcgggcg atcaaggatc atcggaccgg cggtcgaatg gttttcgtcg
    11881 agaattatga tatggacatt gcccgtcacc tggttcaggg agtggatgtt tggttgaata
    11941 ctccccgtcg tccgatggaa gcatcgggaa cttccgggat gaaggcggct atgaatggtt
    12001 gtttaaactt ctccgttttg gacggttggt ggcatgaagg atataacggg aaaaacggat
    12061 gggcgatcgg cgacgataag acctatgaga gtgatgagaa acaggatgaa atggatgcgt
    12121 tgagtttata cgaaacgttg gaagaccaaa tcgtcccgat ttattattca aaccgttccg
    12181 aaaacggtgt tccgacaaca tggatgaggt gggtgaaaga agcgattcga actttatccc
    12241 cacagttttc gactcgtaga atggtcggag attatgttcg cgaaatgtat attcccgcga
    12301 taaaggaatc cgaggaataa gacagatcgc gatcggcaga cagatagcgg ttgtttcgaa
    12361 tcgatatttt tccggacagt gaattatcct ttgtttgtga accaaacggg ttaattttga
    12421 taatcgttat ttcggaagga tatctgtttc tgtttaccga atgaattaag agaggaaaaa
    12481 atggatttta cagtttccaa agatcttata atcggcggct tggcaatttt tctgctgcgt
    12541 atttgtgata cctccatgga tacgctacgc gttttatttg ttgtgcgagg taaaaagtct
    12601 atcgtatggg ttttgggagt ttttcaatcg acgattttta ttatcgccat cagcagcgcg
    12661 ctgaaagggg ataataatct ttttactatt ttggggtatg ccaccggttt cgcgaccggc
    12721 aatgtgatcg gtatggctat cgaagatcac ttagctatcg gatttaagcg aatatccatc
    12781 gtaacgcgtg ataaaggcga agaaatcgca aaagcgatcc gagatgccgg ctatggcgtc
    12841 accgaaatat tcggacgcgg tcgcgacggg gtcgtgacta tgattaatgt aaacgccaag
    12901 cggaaacatg tcaaagatat cgaaaatatt gctttagcag tggatgagac ttcattcatc
    12961 actgtcgatg atttcagtcc ggtcaatcac agcggttttt ggcgaaagta gtttatgaaa
    13021 atcaataaaa aaatcagcca tctttacaga tggctgacac ttcttaaaat gcagaccctt
    13081 tcgaaaaaga gctgctgtag ttcccgccac gccccctcat taggaacccc ttgcacaacg
    13141 ttatcgtaac atattaataa atgatttgac aagtcatccg attacttctt tcctgaaata
    13201 aaggaatttt gtaatatatt ttgcatcgaa tcacggaaga gattttcatc cgaacttttt
    13261 ttcaccaaaa aataagaaac gcgtcctcga tcgcccaaga gatttttaac gttttcccga
    13321 aaatttgaat ctttttcaat ttcgtcgttt atttacaatt cactttatat acggataagt
    13381 tttcggcaaa acggatggca atgaattgag cgtcatggga ccaggcagtc tggaggactt
    13441 tgtcggcggt aatcggaagt tcgaattgtt ctttggtttt atcatttaaa atccaagtct
    13501 tattttgatc atccgtataa aacaccatac ttaaatccgg tgaccagtta ggttggttca
    13561 ttgccggata ttcacgttcg tcatattccg gaacgttttg cggaaaatct ctgccggtgt
    13621 gccctttttt ccggacttca tcgatcaatc gggttgtgcc ggtcaccgta ttgatccgat
    13681 aaatttgatc gcaatcgatg ccgcaactgg tcgtaacggt aacttcatca tcattttccc
    13741 attggatgac cgatttcgag ctgctgtaat tatccgtacg agccgatgta tcggtaaata
    13801 agtcaatggt cgtctggagg tttgacgaaa gcgtcgctac cactacggta taaatcttct
    13861 ccgaggtacg caaaacaaca aaggcaattt tggtggcact tgggttaaat gcgaaatctc
    13921 cgaaaacttt cagcccggtg gtattcataa cggtcgggtt cttataatct ttttcaaaat
    13981 ccacgatttc cgcatctccg gaaaaccatg cccaatagcg gttttccgga acaacgaaag
    14041 caagcaaatt cttatttccc gcccaggcaa aaattcctcc gtcctcggca tccgcgaaag
    14101 tggatatgct ggggctgtcg aatattaaac aggaacgatc ttccagtacc ttattccctt
    14161 tcagatcaag tgtcggctgg acggaagtgg gtgttaccaa agctgttaat gtcgcacttt
    14221 gaggatccac ttcctgtaaa cctgtgagta tgctgcaaga agcaaaaagg aagacgaaaa
    14281 tacatccgat gccaaacagc actaacaatt tatctgtcaa tttattgatt cgcatgcgaa
    14341 aagtttaact catccttgta aaaaaattac aactttaatt tttgctatga ttccttcggc
    14401 agattatccg tcggttcatc aaagaaaatt tcacgataac cgtaacaatt gatgactttg
    14461 gtgttgtaat aaacagtttc ccggaccgtg tcggaacgat aaacatggat gtgcccatgc
    14521 aaatgaattt tcggtttaaa tactttatcc aaccaacgaa atgcacgaat tccgttatgc
    14581 ggcaagtctt gctgatcgtg aatctgccaa ggcggggcat gagatacgaa tagatcaaga
    14641 tatctcccat gaagcagttt gtttcttaaa agagccggta ccaatgaaaa tacatttgtc
    14701 cacatttcgc tctgagaata ttgttgcgga ccgttgttgt atttcaccga tccctcgaca
    14761 ccggcaatca ataagcgctt ttcataaaag taattggaac aatgaaggtt atatccgctg
    14821 gaattataag ggtaattgga ggttttactg accggaatca agctgttatg attgcctaat
    14881 acaaaataca gcggggcgtt taatgttgtg gctgcgtagt ccagatagct catcggtaaa
    14941 tccccacaac tgacgattaa atccacagcg gggaagtatt catcggaacg ttcgctgtat
    15001 atcaagccgg tttccacatc cgaaagcgct aaaattttta tcgtcatcca ttcattatat
    15061 gatgaatgaa ataaaaatct tgcggatttt gtcgattcaa tcggaagcga tcgctattgc
    15121 cacggcatag tttttgacat gactgatact tacagaccaa attgacaatc ccagagattt
    15181 tgccaaagcg gctgcgttgc cgtataagta taaaatcggc atttgttgat catttgtcaa
    15241 aatttcaatc tcctgccaat gaacggttcc gatgccggta ccgagcgctt tggaaacggc
    15301 ttctttacag gcaaatcgcc ccgccaatcg ttcatcgcga tccccgcata aatccagctc
    15361 cgccgaggtg tagactcttt gtaaaaaacg agcttttatc tccggtttta atttctcaat
    15421 ccgttcgatt tcgatcagat cgattccgga tcgcagaatc gttgtcatgc ttttccccgt
    15481 aaagtcccgg ccgttgccaa agtaagtcga tccggatcca gataagtcgc ggcggtttcg
    15541 agaatctgtt cccgtgttac cgcccggatg atgtctccgt accggcgtaa ataatcgagt
    15601 cccaactgat atcgttcgat cgacagcagt tcgtttgcga ttccggcgtt gctctccaat
    15661 tgcaggggca ttcttcctaa gtagtatgat tgaacatcgg ataattcatt cggttcaacc
    15721 ggtgtgctga caaatttttg aatttcggaa cgaatcagat cgacggttcg atcgagattt
    15781 tccggattga ttccggcgga aatttcccag gacccgccta acggcaaaca atttacatcg
    15841 gaaccggcat aataagccaa cccgttttca tcacggacaa ctctgccgat acgccccatc
    15901 attccgaatt ctcccaaaat gcaatttccc aagcgagccg gctgatactg cggagatttt
    15961 cgctccggtg cgatgctgcc gatcaccaga tccaactgac tcttttccgg aatctcaatg
    16021 tgtgtggcgc ttcctttttc gggttttgtg actttcggaa aatccggcaa atttggaatg
    16081 gcctgaggtc ccgcttccca atccgaaaaa tatttccgaa ccaaatctgc cgccttctcg
    16141 ggttcgattc cgcccacgat gctgataacc attccttgcg gcttgaaata ctgagcatgg
    16201 tgacgtacca aatcagatcg tgaaacggat tgcacgcttt ccggtgtccc cagtttatct
    16261 cgcccgtacg gatgggaacc gaataaagct tggtcgaagg cgacatttgc catttcagcg
    16321 gtatcctgtg aacggattgc caaagcggtc agcatttggt cacggatttg ggtcaggtga
    16381 ttttccggaa atattgggtg gcgtaaaata tcattgagta agtcaagcat ggccggcaaa
    16441 tcttccgcca aggattgtcc cgcgaaactg aaagaatgga agccggcgtt gaaacccaaa
    16501 gatgcaccgt tatcttccaa cttgcggaaa atctgttcaa aactatttgt ttcggttccg
    16561 cgcatcaatg cgagagacag aaaaattgcc agaccgcttc gatcgaccgg atccagaacg
    16621 gatccgccgg gaaggtatcc gctgatcaca acggatgggc tgaggctgtt ttctctggca
    16681 aggagtacga tctgattcgg aagttctatt cgcagaatgt cgtcactccc cggaattgat
    16741 tggatatttt tcatgattcc tcactttctt ccgggtaagg gcgataaatc ccgatcaccc
    16801 gatgagccga gttgagatag gttgaagccg atttggcgac atccgaaacg gttaccttat
    16861 tcaaagacga tacgaattcc gtaaaccagg aataagaagc gaacatttcc gtataaccca
    16921 tccaataggc ttgattggtg atgttttccg atccgtaagc gaataacgca cgggcttgtt
    16981 ttaccgcttt ttttaattct tgctcgctga tatctccgga ttgaatccga cttatttctt
    17041 cgtcaattat ccgaacagct tcctgaggat catgatccgg atgcataata acggtcatca
    17101 tataaaggta aggatcgatc gtggcattgt aatctccgct gaatgcgacg gcaatctgtc
    17161 gatcgatcaa aggttgatat aagcggctgg ttttatttga aatcgatccg cttccgaaca
    17221 tattcagact gctgggaccg gtcagaatac tgtccaacac agccaacgga aagatatccg
    17281 gatctctgcc tgccggtgcc cgccatacga tgcgaagata ggttgtatcc ccgggtcccg
    17341 agagttcgac gtgccgttcc tcgggaatgg ctttttcggg ttggatcgtc accgagggaa
    17401 tgggtctggc tgaaacggat tgatatacgg cggccaatcg atccaacatt tcgttcgcat
    17461 cgaaatcgcc ggccattgtc aggaccgcat tccccggagc ataaaattgc cggtaatgtg
    17521 tataaagatc atcccgagta agagttaaca aatcggattt ttctccgatg atctcaaaac
    17581 gataaggatg acgatcaaat gcagccttgt ctatcgcttc atttaatcgg aaaagcggtt
    17641 cattttccga tccctcgcgt tcggaaagga taacggtgcg ttccgattcc acttcttccg
    17701 gcaggaacag gctgttttgc atacgatcgg cttcaagatc aatcggtaaa gcgattttat
    17761 ccttcggcat ggttgaaaag aaagccgtcc aatccaggta ggtgaatgca ttccagatgc
    17821 ctccgccgcg cgaaattgcc tggtccagca cacctgccgg atatttggcg gtccctttaa
    17881 actgcatatg ctccacccaa tgagagattc ccgtttttcc ggggacttcg tttcgcgatc
    17941 cgacacgata ccagatccaa tggctgataa tcggagcact gtgaatttct tttaataaaa
    18001 cggtcagacc gttttccaat tttgtttgca cgatttcatc gctcatgaaa ttgcggtttc
    18061 ctcctttggg ttttccgtcg tccgacttaa attaaccatt ctattctaaa acaaaataaa
    18121 agtctgcgaa cctttttgta ggttccgcag acttttttta tgatatcccg aatctcggaa
    18181 attgattttc ggattattgt tggctgacgg cttcgatttg aacttccggc acagaatcgg
    18241 ttgatgtaac cgggaagtcc gcgttccaga aatagttata accgtaaaat ccgcaggaac
    18301 ccttgtcttc gaatctgacg atgatcgggc tgtacccttt caactcttcc ggaatgtcat
    18361 aggaaacagt ctgcggtgaa ccgtcggcgg aatcgtaggt accgacttcg aatccgacga
    18421 aataagtcgg gatcaaaccg gcgtgcggtg tcgcgccgcc aagatggaaa ttaacacagc
    18481 cgcttgttcc gcaacccggg accatcggtc cgaacatcca agaaaaagat ggatcgggaa
    18541 tcgggtacat atccggttcc ggagtcccat cgacatccat ccatggatcc caaggatcgg
    18601 aggtggggca ttcgtgtccg ccgcaatgat ggtgcgcagc gggagctccg gcagcataat
    18661 aacccatttt taccgtgaag gactgattgg cggggaaggt accggtggtg acagtgatgc
    18721 tctttccttt aacgacgctg tcgattgtga aggaaggaag aacactgtaa tcgcaaactg
    18781 tcgcaacagt ttccgtcatg gcttcgttgc tgaatgtgct gggaatttca attccgttat
    18841 tgttgctcag cagcataccg atgcttttct tcccgaccag ttccgcggga ataggaaatg
    18901 tggctgtaaa actgcccccg gattgagtgt tgaattttgc gacgtcaata taatcgctga
    18961 ctgaatttaa gtctttcatg cggacaatga actcggtgtc agcagggaaa ttaagggtta
    19021 aaatgctgac actttgatcg actgttacac tggtaatagt gtaagaagga tagggttcaa
    19081 tcccttccgc ttgaactgtt gcgataccaa ttgcaaacat cgcaaccaat atcataagaa
    19141 cgaaaagttg ttttttgttt ttcatcatta ttgcctccgg catataaata atacaaagtg
    19201 ttatacaatc caagcagtca ggatcgagct gcttcgtata caatctccag tcagcttcta
    19261 ttcaaaataa gctgattgcc ttcgtagaat aatggtgcaa gaaacgaacc aaattgatat
    19321 caggattgta aaggcttcgc tttttcaata ttcagataac ataaaatgcc gataacaatg
    19381 aaaccgaatc ccccgacgat tcccaccgga tagggtccta aaaacggttc gggggttcct
    19441 ctgaggaaga aaatcccgac ggacgccatt ccgttgacgg ctccatgggc aatcgcagcc
    19501 ggcaaagcac tctccattcg gaatgtaagc cacgcgagaa acgaacccac aaaaacgcaa
    19561 aataaaatca tcccgataat tccgagccag gggaacaatg gataattttc cccgtagtta
    19621 tatcccatcg cgataatggg agcatgccag attccccaaa caactccgct gattacaacc
    19681 gcttttttcc gcggaagaaa atccgccagt ttcggcaata aataagcgcg ccaaccgatt
    19741 tcttctccga gacatgggat cgcatttaaa atcggtgaca tgatcagcgc caacaaaacc
    19801 tgcgaaagga tggataaaag caatttctgt gtcgtaagtt cggctgtttg ctgttccccc
    19861 aacgcgttca acgattgccg taaaacagac atttcgggat caaattttgc cgggaaaatc
    19921 aggtaataga gcgccgaacc gaacaggatc aacaaagccg gtccgaacca agcgatcaaa
    19981 taggtcttga tattcgcttt aaaatccaat cgcagccata gattctccaa tccttctttg
    20041 gtaatccaac gggtcagaaa agaaccgatc atcggaatca tcataataat ggaaaggtag
    20101 atttgaactc tcggattttt caatccgccg cctttccaaa gcatgaattc ggcagcccat
    20161 gtgatcacga acgtgatgat gataaaaatc atcgtccttt tcttggatag attcatttga
    20221 ttggttcctt cctgtcagct gatatatcga tacgaagctt tcattaatat tcaataccga
    20281 tctgggcggg tatgcgttgg gtggtataag ggtgtttaat ttctttcatc tccgttacca
    20341 aatccgcaaa atcaatcagt tcctgcggcg cgtttcgacc ggtcagaatt aagtgcatgg
    20401 tcggaggttt attctcccgg atccattgaa taaattcatt acaatccagc catttataaa
    20461 cgaatagata ggtaatctca tccaacagca gcatatccaa atttcgatgc aggattactt
    20521 cccgagtaaa atcccaagcg gaacgggcag cttccgcatg aatggttgta tcaaattttt
    20581 cgaaaacaaa tcctttcccg agtgtttcaa aaggaatacc cagccgccgg caagcgatca
    20641 tctcaccctt gacaatttcg ggcgatttaa taaactgaat accggcgaca ctcatattat
    20701 ttccccaggc tcgaaaaagt aatcccatcg cagcggtagt tttgcctttc ccgttcccgg
    20761 tataaagaat attcaatcca tgggtcaaac ttggttgcat cgttattgcc gtgcctttct
    20821 gttcgtcggt tccaattata agcgaaatag ccatattcaa tcgaaagggc gctcttatat
    20881 tctgtttcga aatatagagc gccctctctt ccttttgatt agttcaggcg attaatccca
    20941 atgtaaaata ctctctcgta tctttcccca ctc
//
LOCUS       DAOSMM010000039        20587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2590_length_20587_cov_17.511884, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000039 DAOSMM010000000
VERSION     DAOSMM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 20587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20587
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2590_length_20587_cov_17.511884"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..569)
                     /gene="pdxT"
                     /locus_tag="PK898_06715"
     CDS             complement(6..569)
                     /gene="pdxT"
                     /locus_tag="PK898_06715"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOU19761.1"
                     /translation="MTKIGVLALQGAFIEHCQILRRLAVDCVELRKPADISQDLDGLI
                     LPGGESTVMGKLLNDLGMLIPIRKMISEGLPVFGTCAGMILLAQRLSEDSIPRPGTMD
                     ITVRRNAYGRQIGSFNTVGTFAGKRVPMTFIRAPYIEEAGTDVQILSVEDGHIVAAEQ
                     GNQLVTAFHPELTDDPTVHQHFLDMIS"
     gene            complement(566..1444)
                     /gene="pdxS"
                     /locus_tag="PK898_06720"
     CDS             complement(566..1444)
                     /gene="pdxS"
                     /locus_tag="PK898_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOU19762.1"
                     /translation="MVENDRYSLNKQLAQMLKGGVIMDVTSPEQARIAEDAGACAVMA
                     LERIPADIRAAGGVSRMSDPKMIKSIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYID
                     ESEVLSPADDIYHIDKTKFKVPFVCGARDLGEALRRINEGASMIRTKGEPGTGDIIQA
                     VRHMRKMNAEIRRIQSISEDELFEAAKVLEVPYDLLKNVHENGRLPVVNFAAGGIATP
                     ADAALMMQLGAEGVFVGSGIFKSGNPAKRANAIVRAVTNYQDPILLAELSEDLGEAMV
                     GINENEIAILMEERGK"
     gene            1551..2948
                     /locus_tag="PK898_06725"
     CDS             1551..2948
                     /locus_tag="PK898_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU19763.1"
                     /translation="MLTYLLEHDKQKPLYLCLYAFIRKDIESGILKPNEKLPSKRNLA
                     EHLHISVITVQNAYTQLIVEGYIYPIARKGYYVVPLQQTVPLTSEHRETKGILPRRQE
                     NKIRFDFKSNSTIPDQFPFTIWAKLMREVLSERNPELLESGPSNGNEKLRISIATYLH
                     DFRGMTVSADRIVIGAGAEYLYSLVVQLLGRDKVYAVENPGYQKIAKIYRNNGAVCLP
                     IRTDDHGISAETLQSSDAEVVHITPAHHFPLGIVMPISRRNELLAWANEEEDRIIIED
                     DYDSEFRFQGKPVQPMINIDQNQKVIYVNTFSKTLFPSLRISYMVLPDRFMQIYNQKL
                     GFYSSTVPSFDQLVLANFIAGGYYEQHINRMRNYYKRLRNQLIFSLRQSSLQDRIQII
                     EEDSGLHFLLKVDTTMKDEEIVKKAEILGINLSCLSEYYFDRNSRENGTLLINYSGIS
                     IEQVDEAIETLSAIF"
     gene            complement(3012..3494)
                     /locus_tag="PK898_06730"
     CDS             complement(3012..3494)
                     /locus_tag="PK898_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU19764.1"
                     /translation="MKSNFSIRTASSADVKLILQFIRELADYEKMADQVTATEELLSE
                     WLFEKRIATVLIGESEGMPVGFALYFYNFSTFLGKAGIYLEDLYIREEYRHRGFGTEF
                     MKALARIATENNCGRLEWWCLDWNQPGIDFYLSLGARPMEDWTVYRITGQSLVELANS
                     "
     gene            complement(3665..4060)
                     /gene="rpsI"
                     /locus_tag="PK898_06735"
     CDS             complement(3665..4060)
                     /gene="rpsI"
                     /locus_tag="PK898_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOU19765.1"
                     /translation="MVGRYYEGIGRRKESVARVRISSGSGDFVVNDKQIADYFNRLGD
                     VESILAPFTAINQDQSGFDVSVHVNGGGVTGQTEAVRLGLARALVKMDDTLKPSLSHA
                     GLLTRDPRIKERKKPGLKRARKAPTYTKR"
     gene            complement(4072..4503)
                     /gene="rplM"
                     /locus_tag="PK898_06740"
     CDS             complement(4072..4503)
                     /gene="rplM"
                     /locus_tag="PK898_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOU19766.1"
                     /translation="MTVEKTYVIKGEPESEWLLVDANGQSIGRLATQIASYLLGKHKP
                     TYSPGVKMGDFVVVINADHLAIPQKKLATKHYYRHSGYPGGLTDTTLKDMMRTYPERV
                     IESAVWGMLPHNKYGRALMKRLKIYAGNEHPHAAQNPTRVN"
     gene            complement(4535..5320)
                     /gene="truA"
                     /locus_tag="PK898_06745"
     CDS             complement(4535..5320)
                     /gene="truA"
                     /locus_tag="PK898_06745"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOU19767.1"
                     /translation="MSELSERGNPVPDTCERYKFTVSYDGTLYWGFQRQKGRRTIQSE
                     LETALTALGWKETGIISAGRTDSGVHADGQVFSARLAWQHSPEALRAALNARLPQDIS
                     IKDVKRVPDNFHARYDAFSRTYRYRIYFAEVRDPLRDRFRLRLWPESDLKTLNQAAHI
                     LLGEHDFRAFGSPPRKNGTTVRRVIASGWIPEDRDEIAFEIEANGFLYHMVRRMVFLQ
                     LQWVTDRIDWKTWEDAVLNAKPTHPGIAPAKGLSLYRVRYEKL"
     gene            complement(5313..5693)
                     /gene="rplQ"
                     /locus_tag="PK898_06750"
     CDS             complement(5313..5693)
                     /gene="rplQ"
                     /locus_tag="PK898_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOU19768.1"
                     /translation="MRHGVAGYKLGRTSSQRKLLRMTQISQLFEHERIITTQAKALSM
                     RNDAEKLITLAKKGNQGDKIQKMNAARLASSRLGNDRELTKKLFDDIAPRYDKRNGGY
                     TRMIKLGPRKGDAAEMVVIELVGE"
     gene            complement(5699..6664)
                     /locus_tag="PK898_06755"
     CDS             complement(5699..6664)
                     /locus_tag="PK898_06755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOU19769.1"
                     /translation="MATLSNMVTPKVEREREAENYGKFVVYPLERGYGVTLGNALRRV
                     LLSSLEGVAVTSLHISDVMHEFSTIPGVREDVLQVMLQIKQIRLKMYEGDVSHLRLEV
                     NGEGEVTASDIQCPAEVEILNPELYLFTVDNPDTHLEIDMTVERGRGYLPANDRTNRL
                     PIGELPVDAIFSPIKRVNWNVGSALYGQSTNYDKLELEIWTDGTIQPQAALILASKIL
                     IEHLRYISGITEEVLPDTIVTEQHSTHVTSETAETPIESLDLSVRVFNSLKRTGVATV
                     GDVLDLLDKGDDAVKSIRNFGEKSLEELREKLQEKGYLKKESDTE"
     gene            complement(6721..7362)
                     /gene="rpsD"
                     /locus_tag="PK898_06760"
     CDS             complement(6721..7362)
                     /gene="rpsD"
                     /locus_tag="PK898_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU19770.1"
                     /translation="MARYTGSVCKLCRRENEKLFLKGERCFSAKCSFEKRAYAPGDHG
                     RSGGSRGGERVSDYSRQLRAKQKVRRKYGVLERQFRRVFGIAVRQSGLTGLNLLQLLE
                     TRLDNVVYRLGYAENRAQARQMVTHGHFEVNGIPSDIPSMILKPGDQISVRKSSASKA
                     LFSDLADIADKRVCPLWLDRNTKALSGRVLRLPERSEIDGNINEQLIVEFYSR"
     gene            complement(7384..7788)
                     /gene="rpsK"
                     /locus_tag="PK898_06765"
     CDS             complement(7384..7788)
                     /gene="rpsK"
                     /locus_tag="PK898_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU19771.1"
                     /translation="MAQNVRSSRRASTARKVKRTLGSAQVHIFASFNNTIVTVTDAMG
                     NPVCWSSSGASGYKGSRKSTPFAARLAAEQAIKTAQTMGVQEVDLIIKGPGPGRESAI
                     RAVQGMGMKVRSISDITPVPHNGCRPPKKRRV"
     gene            complement(7910..8299)
                     /gene="rpsM"
                     /locus_tag="PK898_06770"
     CDS             complement(7910..8299)
                     /gene="rpsM"
                     /locus_tag="PK898_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU19772.1"
                     /translation="MARIEGVDLPRNKRIEIGLTYIYGIGLTRAQQALRDTGVNPDIR
                     VKDLSENDENLLREYIAKNYVVEGDLRREVQMNIKRLVEIGCYRGIRHRRNLPCRGQR
                     TRTNARTAKGPKKTVAGRGRRRGATKK"
     gene            complement(8306..8419)
                     /gene="rpmJ"
                     /locus_tag="PK898_06775"
     CDS             complement(8306..8419)
                     /gene="rpmJ"
                     /locus_tag="PK898_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOU19773.1"
                     /translation="MKVTSSIRKRCAKCKIVKRSGRLYVICENPKHKQRQG"
     gene            complement(8519..9337)
                     /gene="map"
                     /locus_tag="PK898_06780"
     CDS             complement(8519..9337)
                     /gene="map"
                     /locus_tag="PK898_06780"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU19774.1"
                     /translation="MSWERQVSIKSKDEIAIMREAGIINAEALKAASEVCVPGATTWD
                     VNAAADRVLDHYKVTSPFKGVPGPIPFPASTCVSVNDVLVHGIPSKKIVLHEGDIVSV
                     DCGTTYKGFVADSAFTIGVGKVSEEAQKLMEVTENSLYIGIEKMVPGNYTGDVSAAIQ
                     EYVESHGFYVTKQYTGHGVGRRMWEAPQVPNYGTKGQGIRLRPGITIAIEPMVLVGTD
                     ATQVHQDGWAVSSADLSLTAHFEHSIAVTEEGPMILTLLENGEPPVSVTGRKKL"
     gene            complement(9354..10016)
                     /locus_tag="PK898_06785"
     CDS             complement(9354..10016)
                     /locus_tag="PK898_06785"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOU19775.1"
                     /translation="MATYIVFLGAPGVGKGTQAERLAEELKIPHISTGDLFRENLKNQ
                     TELGKKAQYYMDAGQLVPDDVTIEMVKSRISRPDCVNGAILDGFPRTSAQAEAFDQML
                     KEAYQTEVDFVPCIQVDRELLIDRLSGRRMCPNGHVFHMVYHPPKEDDICDICGQKLY
                     QREDDKAETIEKRLEVYENQTAPLIDYYQKRNVLRFIDGAKDIDAVTMDILAAFKDVT
                     KK"
     gene            complement(10040..11377)
                     /gene="secY"
                     /locus_tag="PK898_06790"
     CDS             complement(10040..11377)
                     /gene="secY"
                     /locus_tag="PK898_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU19776.1"
                     /translation="MKRSPWRFLWKSTDIRKKLMLTLGILVLYRLIANIPVPGFNAEV
                     IKALKSNTTAAGDLISILNVLSGGTLSSFSIMAMGVYPYITASIILQLLGPIIPAIER
                     KMKDDPREGQKWMEKWTMILTIPMAILSAIGQISLFNQIVPGTTVLRDYGWSGSNFLP
                     TLANLSTMVAGTMVAIWLGQIISEYGIQQQGLSLIIFSGIVSQIPNNLVRILADKTTG
                     WIVLVISLVVLLISVFAIVYIQQGTRYVKLMIPGQRIGYRGIKSGGSTTLPLRVNSVG
                     MIPLIFAQSIITFPALIASFFVNSGTAWVKNSSVWIQSHLSGSSSLYVVLYFVLVVAF
                     TYFYTSVTFMNNDYGSSLKKQGAVVPGRAAGTETQKYLAKIQNRITLPGAVMLGVIAV
                     LPNILAVILHTSAQATMLMSSSGMLIVVGVVRDIIDYLQSELSQHGYEEKLIR"
     gene            complement(11426..11878)
                     /gene="rplO"
                     /locus_tag="PK898_06795"
     CDS             complement(11426..11878)
                     /gene="rplO"
                     /locus_tag="PK898_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU19777.1"
                     /translation="MKLDELRPNEGAKHQKKRVGRGLAAGQGKTAGKGTKGQHVRQGY
                     GGHIYRQGGNLPFYRHLPFMRGEGFTPPNRVEFNEVNLEMLERFDGDIEITPEVLAEA
                     NLLSKKNNPVVILGNGEVSHAYKVKVHRISKGAREKIEAAGGSVEIIG"
     gene            complement(11881..12087)
                     /gene="rpmD"
                     /locus_tag="PK898_06800"
     CDS             complement(11881..12087)
                     /gene="rpmD"
                     /locus_tag="PK898_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU19778.1"
                     /translation="MDKDTSSPKKLRITYVKSAIGYSWKHKDTIKALGFKHLNDSIVR
                     EDTPSLRGMLRKVNHLVKVEELAE"
     gene            complement(12080..12610)
                     /gene="rpsE"
                     /locus_tag="PK898_06805"
     CDS             complement(12080..12610)
                     /gene="rpsE"
                     /locus_tag="PK898_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002063426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOU19779.1"
                     /translation="MENLDYQPLDEQLDERVIEIARVAKVVKGGRRFQFRVTVVVGDN
                     NGTIGLGIGKANAVPDAMRKASERARKNMRKINLYKTTIPHEVIGHVGGAQVLLRPAS
                     EGTGVISAGGVRAVLEVAGIHDILTKSLGSSNLLNVVKATFEALNELKNAEEEAILRG
                     KQPDELLPFWERRKNG"
     gene            complement(12634..12999)
                     /gene="rplR"
                     /locus_tag="PK898_06810"
     CDS             complement(12634..12999)
                     /gene="rplR"
                     /locus_tag="PK898_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOU19780.1"
                     /translation="MAKFSRSEARIRRHARVRKDLAGSAQRPRLSVYRSLNGIYAQII
                     NDETGTTLVSASNIDRGLKADMDGKNKTEQAKLVGKAVAERAKEKGITTVVFDRGGFR
                     YMGRIKALADAAREAGLEF"
     gene            complement(13012..13560)
                     /gene="rplF"
                     /locus_tag="PK898_06815"
     CDS             complement(13012..13560)
                     /gene="rplF"
                     /locus_tag="PK898_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOU19781.1"
                     /translation="MSRIGRLPITVPAGVKVDINGNEVTVKGPKGEMKRLFSPVVKID
                     QDNGTLTVTRSSDEAAIRALHGTSRAILNNMVVGVSQGFTVILEVDGVGYRAEMNGKN
                     LMLYVGYSAPKEVVPPEGISFDVDTKTRQIKVMGYDREQVGQVAAEIRFIRPPEPYKG
                     KGIHYLGEKIRRKAGKSGKAKK"
     gene            complement(13578..13988)
                     /gene="rpsH"
                     /locus_tag="PK898_06820"
     CDS             complement(13578..13988)
                     /gene="rpsH"
                     /locus_tag="PK898_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOU19782.1"
                     /translation="MVNDPIADMLTRIRNAMMAGHATVVMPCSNIKIEIARILKEEGF
                     LESYEVLEGDKPSQRALKLKIKYAGERRSRRPVMSGIVRVSRPGRRVYTGRNEIPWVL
                     SGLGVAIMSTPKGVMTGQRARQLGVGGEILCKVW"
     gene            complement(14011..14193)
                     /locus_tag="PK898_06825"
     CDS             complement(14011..14193)
                     /locus_tag="PK898_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOU19783.1"
                     /translation="MAKKCMMYRELRREYPNQVRNRCKRCGRPRGYMRRFGLCRICFR
                     ELALNGQITGVTKSSW"
     gene            complement(14195..14737)
                     /gene="rplE"
                     /locus_tag="PK898_06830"
     CDS             complement(14195..14737)
                     /gene="rplE"
                     /locus_tag="PK898_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOU19784.1"
                     /translation="MNHMKEKYLQEVLPAMKSTLKLDNPMQYPKIEKVVVNIGLGEAL
                     DNPKALDAAVNDLAQITGQKPVVTKARVSIANFKLREGRQIGAKVTLRGEKMWGFLDR
                     LLNIVLPRVRDFRGVPTDAFDGRGNYTLGLKEQTLFPEIDYEKVDKVRGMEVTIVTTA
                     QNDDQARVMLDLLGMPFRKD"
     gene            complement(14754..15092)
                     /gene="rplX"
                     /locus_tag="PK898_06835"
     CDS             complement(14754..15092)
                     /gene="rplX"
                     /locus_tag="PK898_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOU19785.1"
                     /translation="MKLKIRKGDTVEIISGRSEDKKKRGEVIRVIPKESRIVVQGVNI
                     RSRHQGQTRAGRRQIDAGIVKFEAPIHISNAMLVCPNCNKPTRVGLSRGEDGKVTRIC
                     KKCNSEIDAK"
     gene            complement(15116..15487)
                     /gene="rplN"
                     /locus_tag="PK898_06840"
     CDS             complement(15116..15487)
                     /gene="rplN"
                     /locus_tag="PK898_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOU19786.1"
                     /translation="MIQKESRLKVTDNTGAKEILVIHIMGGSVHHYGHVGDIVVGTVK
                     SASPHGSVKKSDIVKAVIVRCSKEWRREDGSTIRFDDNAAVILDVNGTDPKGTRIFGP
                     VARELREKGFTKIVSLAPETI"
     gene            complement(15484..15798)
                     /gene="rpsQ"
                     /locus_tag="PK898_06845"
     CDS             complement(15484..15798)
                     /gene="rpsQ"
                     /locus_tag="PK898_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOU19787.1"
                     /translation="MNARRRIMGTVTSDKMDKTVVVEIRRSYRHPLVGKVVHDVKHVK
                     AHDENNECKVGDKVMIVESQPLSKEKRWVVQSVIRRDEKKEIIEEVSSELAEAMNEGA
                     KE"
     gene            complement(15795..16007)
                     /gene="rpmC"
                     /locus_tag="PK898_06850"
     CDS             complement(15795..16007)
                     /gene="rpmC"
                     /locus_tag="PK898_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOU19788.1"
                     /translation="MKAAEIRLLTKDEITAKLSDLRQEKMNLRFQAVSGQLSDLSRVK
                     EVRRDIARMETILREMEMQKQQEGKA"
     gene            complement(16004..16438)
                     /gene="rplP"
                     /locus_tag="PK898_06855"
     CDS             complement(16004..16438)
                     /gene="rplP"
                     /locus_tag="PK898_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOU19789.1"
                     /translation="MLMPKRVKYRKQQRGRMRGKALRGAEITFGEYGLMALEPGWVTA
                     RQIEAARRTLAREMKRHGKVWIRIFPDKPYTHRPPETRMGKGKGNVEYWVAVVKPGRI
                     MFEIGGLGEEAALEALHQAAYKFSIRTKVVSRHELGTGEEQS"
     gene            complement(16506..17186)
                     /gene="rpsC"
                     /locus_tag="PK898_06860"
     CDS             complement(16506..17186)
                     /gene="rpsC"
                     /locus_tag="PK898_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOU19790.1"
                     /translation="MGRKVHPIGFRLNINKPWDGRWYATGNEYVDQLHQDLQIRNMIH
                     EQTGKAGVSKVEVERFPGKVKVVVNTAKPGILIGRKGETVKIIRQNLEALTGKKVDLE
                     IKEIKSPDLDAYLVAMNIAGQIERRVGYRRAMKRAIQQGLRQGAGGVKIQVGGRLSGA
                     DMARTVTLREGRVPLQTLRADIDFARAEANTTYGKIGIKVWIYRGIVLSMNEEAAAES
                     TEGVYVSE"
     gene            complement(17228..17569)
                     /gene="rplV"
                     /locus_tag="PK898_06865"
     CDS             complement(17228..17569)
                     /gene="rplV"
                     /locus_tag="PK898_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOU19791.1"
                     /translation="MAQDVIAKLNNCGVSAQKVRLVINLVRGKSVTDALNILKFTNKV
                     SAEPVYKLIASAASNAEENFGLDRNDLYVHSIYCDEASTRRWRYFGARGRFKPVLRRS
                     SHVTVVLRERE"
     gene            complement(17575..17850)
                     /gene="rpsS"
                     /locus_tag="PK898_06870"
     CDS             complement(17575..17850)
                     /gene="rpsS"
                     /locus_tag="PK898_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOU19792.1"
                     /translation="MSRSLKKGPYVDPKLLKKVEKMNDAGEKKVIRTWSRASTIFPQM
                     VGHTIAVHDGRRHIPVYITENMVGHRLGEFAPTRLFRGHVSEKATKA"
     gene            complement(17886..18725)
                     /gene="rplB"
                     /locus_tag="PK898_06875"
     CDS             complement(17886..18725)
                     /gene="rplB"
                     /locus_tag="PK898_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOU19793.1"
                     /translation="MAVKIYKPITPGLRGMTGYTFEEITKDKPERSLIVIRKNHAGRN
                     SDGRITVRHRGGGERRYIRIVDMRRDKIDVPAKVIAIEYDPNRTARIALVQYKDGEKR
                     YILAPIGLKVNDSVVSGPKVEIRTGNAMPISNIPVGSTIHNIEMKPGKGGQLVRAAGS
                     SAQLLAKEGDYAQIRLPSGEVRLILQTCYATIGQLGNLDHSNVKLGKAGRKRHLGIRP
                     TVRGTAMSPRDHPHGGGEGRQPVGMPGPKTPWGKPALGYKTRSNKKTDKYIVRRRNAK
                     SGR"
     gene            complement(18725..19027)
                     /gene="rplW"
                     /locus_tag="PK898_06880"
     CDS             complement(18725..19027)
                     /gene="rplW"
                     /locus_tag="PK898_06880"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOU19794.1"
                     /translation="MTTDFEILRRPLITEKTNEMAFKLNQYVFEVDPDANRTMVKEAV
                     ESIFKVNVVRVNIIRTPGKSKRNTKNRRLFLKKPVMKKAIVTLAAGDKIPFFEGVE"
     gene            complement(19038..19664)
                     /gene="rplD"
                     /locus_tag="PK898_06885"
     CDS             complement(19038..19664)
                     /gene="rplD"
                     /locus_tag="PK898_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOU19795.1"
                     /translation="MKIDVKNMKGEVVNQIELPETIFAAPIYMDLMHQAYVRQMANAR
                     LGTHETKVRHEVAGGGRKPYKQKGTGRARQGSIRAAQWKGGGRVHTPHMRDYTQDMPK
                     KMRRAALRSALSVKANDSEIVVVDELKLDEPKTRLMANALSLLVGDNSALILIPDKES
                     SVDVIRATNNLPYAKILLANYLNIRDLLRFDRIVIPVAAIETINSFLG"
     gene            complement(19698..20327)
                     /gene="rplC"
                     /locus_tag="PK898_06890"
     CDS             complement(19698..20327)
                     /gene="rplC"
                     /locus_tag="PK898_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOU19796.1"
                     /translation="MFKGLLGKKIGMTQIFDENGVAIPITVIEAGPCYVTQLRTQEND
                     GYSAVQLGFEEVKPKRITNAEKGHLKRNNLPPLRFLREFRVKDPQVSEGDKVTVDLFA
                     LGELVDVVGTSKGRGFQGGVKRYNFRGGPKTHGQSDRLRAPGSHGSGTTPGRVFKGSR
                     GPGHMGNDRVTAQNLKVAFIDAERNLIGVKGAVPGARGGLVMIRDARKS"
BASE COUNT         5146 a         5428 c         4199 g         5814 t
ORIGIN      
        1 aaaagctatg aaatcatatc taaaaaatgc tgatgaaccg tcggatcatc cgttaattcc
       61 ggatgaaaag ccgtcaccaa ttggtttcct tgctccgctg ccacgatgtg tccatcctct
      121 acggaaagga tttgaacgtc tgtgcctgct tcttcgatat agggcgcacg aataaaagtc
      181 atgggaaccc gctttccggc aaatgtcccc accgtattaa aactcccgat ttgcctccca
      241 tacgcattcc ggcgaacggt tatatccatc gtccccggac gggggatcga atcttccgaa
      301 agcctttgag ccagtaaaat catcccggca caagtaccga ataccggtaa tccctccgaa
      361 atcattttgc ggatcgggat cagcattccc aagtcattca gcagcttacc catcacagtg
      421 ctttcaccgc cgggcaaaat taaaccgtct aaatcttgag agatatcggc cggtttgcgc
      481 aactctacgc aatcaacagc caaacgacga agaatttgac aatgttcgat aaaggctccc
      541 tgcaaggcaa gcacaccgat tttggtcatt ttccgcgttc ctccatcaga atagcgattt
      601 cattttcgtt gattcccacc attgcttcgc ccaaatcttc ggacaattcc gccaaaagaa
      661 tcggatcctg ataattggtg accgctctga caatggcatt agctcgcttg gcaggatttc
      721 cggatttgaa aattccggaa ccgacaaata caccttccgc tcccagctgc atcattaaag
      781 cggcatccgc cggggtggcg atcccgccgg cggcaaaatt cactacgggc agtctgccgt
      841 tctcatgaac attttttaat aaatcgtatg gtacttccag aaccttggca gcctcaaaca
      901 gctcatcttc cgaaatcgat tggatccggc gaatttccgc attcatcttc cgcatatgac
      961 ggacagcctg aataatatcc cccgttcccg gctcaccttt tgtccgaatc atcgatgctc
     1021 cttcattgat tcggcgtaaa gcttcgccta aatcccttgc gccgcagaca aagggaactt
     1081 tgaattttgt tttatcaata tgataaatgt catcagccgg cgatagaact tcactctcat
     1141 caatatagtc gatttcaatc gcttcaagaa tttgagcctc gacgaaatgt ccgatacgga
     1201 cttttgccat aaccggaata gaaaccgctt cctggatgct tttaatcatt ttcggatcac
     1261 tcatgcgaga gactcctccc gcagcccgaa tatccgccgg aattctttcc aatgccatga
     1321 cggcacaggc tcccgcatct tccgcgattc tggcctgctc cggtgaggta acatccataa
     1381 tcaccccgcc tttcaacatt tgcgctaatt gtttgtttag agaataacga tcattttcaa
     1441 ccattgcaac actcctgatt ttttatagta cctacacaat ttaaccggga tctgatatga
     1501 ttgcaatatc cattttgata taatttatta ataccagttt tggaataact atgcttactt
     1561 atcttttgga acacgacaaa caaaaaccgc tttacctctg cttatatgcc tttatccgaa
     1621 aagatataga aagcgggatt ttgaaaccga atgaaaaact gccatccaaa aggaatttag
     1681 ccgaacatct tcatatcagt gtcatcaccg ttcaaaacgc ttatacgcag ctgattgtgg
     1741 aaggttatat ttatccgatt gcgagaaaag gatattatgt cgttcccctt caacaaaccg
     1801 tccccttgac ttcggaacat cgcgaaacga aagggatctt gccgaggcgt caggagaata
     1861 agatccgatt cgattttaaa tccaacagca ccattccgga tcaattccct tttacaatct
     1921 gggcaaaatt aatgcgtgaa gttttatccg aaagaaatcc ggaactattg gaatccggac
     1981 catccaatgg gaatgaaaag ctccgaattt ccatagcaac ttatttgcat gattttcgcg
     2041 gaatgacggt ttctgcggat cgaattgtga tcggtgccgg agcggagtat ctgtacagtt
     2101 tggtcgtgca gcttttgggc agagataaag tctatgcggt ggaaaacccg ggctatcaga
     2161 aaatcgccaa aatatatcgc aataacggtg ccgtttgtct gccgattcga acagatgatc
     2221 atggaatctc cgcggagacc ttacagtctt cggatgccga ggttgtgcac attactccgg
     2281 ctcatcactt tccgctgggc atcgtcatgc cgatttcgag acggaacgaa ctcttggcat
     2341 gggcaaacga agaagaagac cgaatcatca tcgaagatga ttatgacagc gagtttcgct
     2401 ttcaaggaaa accggtccaa cccatgatca atatcgatca aaatcaaaaa gtaatttacg
     2461 tcaatacctt ttccaagacc ctgtttcctt cgttgcgaat cagttatatg gtattgcccg
     2521 atcgattcat gcaaatatac aaccaaaagt tgggatttta ctcatcaacc gtaccaagtt
     2581 tcgatcaact ggttctggca aattttattg ccggaggcta ttatgaacag catatcaatc
     2641 gcatgcggaa ctattataaa aggttacgaa accaattaat tttttctctg cggcaaagtt
     2701 ctttacaaga tcggattcaa atcattgaag aagattccgg tttgcatttt ttactgaaag
     2761 tagatacaac gatgaaagac gaggaaatcg tcaaaaaagc tgaaattctt gggatcaatt
     2821 tgtcttgtct gtctgaatat tattttgata gaaacagccg tgaaaacggg acattattga
     2881 tcaactactc cggaatatcg atagaacaag tcgatgaagc gatcgaaact ttaagcgcaa
     2941 tattctaaaa ccccaaaacc gcccttgacg tacaaaaaca ggttttataa tctcatctct
     3001 cccattccgc atcaagaatt cgccaattcc accaaggatt ggcctgtaat tcgataaacc
     3061 gtccaatctt ccataggccg ggctcccaaa gaaagataaa aatcgattcc cggctgattc
     3121 caatccaaac accaccattc caaccttccg caattatttt cggttgcgat ccgcgccaat
     3181 gctttcatga attcagtgcc gaaacctcgg tgacgatatt cctcgcgaat atacaaatct
     3241 tccaaataaa tacccgcctt cccgagaaat gtcgaaaaat tataaaaata cagcgcaaaa
     3301 ccgaccggca ttccctctga ttcgccgatt aagacagtgg caatcctttt ttcaaagagc
     3361 cattcggata gcagttcttc ggttgctgtc acttgatctg ccattttttc atagtcagcc
     3421 agttcacgga taaattgcaa aattaactta acatcggcag atgaagcggt tcgaattgag
     3481 aaattgctct tcatcgtttt ttatgcctcc aaaataattg atttttcacc gaaaaatatt
     3541 ttgattttta tcgacgatcc gatcttgttt ttccgatcaa gtggatatcg aactttcctg
     3601 cgatattaaa aaacccgtcc aaactcgaac gggttttcca tagtacaaac tctgacacag
     3661 ataattaacg ttttgtgtaa gtcggtgcct tgcgagcgcg tttcaaaccc ggcttctttc
     3721 gttctttaat ccgtgggtcg cgagtcagta atcccgcatg gctgagtgac ggtttcagcg
     3781 tatcatccat cttaaccaac gcccgtgcca atcccaaccg aaccgcttcg gtttgaccgg
     3841 tcacaccgcc tccgttcaca tgaacagata catcaaaccc tgattgatct tggttaattg
     3901 cggtaaaagg cgcgagaatc gattcgacat cgccgaggcg attgaaataa tccgcgattt
     3961 gcttatcatt taccacaaaa tcaccgcttc ccgaactgat gcgtacccga gcaacactct
     4021 ccttgcgacg tcctattcct tcataatatc gtccgaccat tcttttactc cttaattcac
     4081 acgtgtcgga ttctgagcag cgtgtggatg ctcattacca gcataaattt ttagccgttt
     4141 catcaacgca cgtccatatt tattgtgcgg aagcatgccc caaacagccg attcgataac
     4201 acgttccgga tatgttcgca tcatatcttt taatgttgta tccgttaagc ctccgggata
     4261 accggaatgg cgataataat gtttcgtcgc caatttcttt tggggaattg ccagatgatc
     4321 agcattgatc acaaccacaa aatcgcccat ctttacaccc ggggaatatg tcggtttatg
     4381 tttccccaat aaataacttg caatttgggt tgccaaacgc ccaatgctct gcccgttcgc
     4441 atcaacaagc agccattcgc tttcaggttc acccttaatg acataggtct tttccacagt
     4501 catctctcca attcctaata aacttttttc tgattcacag tttctcatat cggacccggt
     4561 aaagacttaa ccccttcgcg ggagcaattc ccggatgagt cggtttcgca ttcagcaccg
     4621 catcttccca ggtcttccaa tcgattcgat cggtaaccca ttgaagctgc aaaaatacca
     4681 tccgccttac catgtgatag agaaatccat tcgcttctat ttcaaatgcg atctcatctc
     4741 tatcttcggg aatccaaccg gacgctatta ctcgccgaac tgtggtgccg tttttccggg
     4801 gcggggaacc aaaagcacga aaatcatgtt cgccgagcaa aatatgagcg gcttgattca
     4861 gggttttcaa atccgattcg ggccataacc gcagtctgaa acgatcacga agcggatccc
     4921 ggacttcggc aaaataaatt cgatatcgat aggtccggga aaacgcatca tagcgtgcat
     4981 ggaagttatc cggtactcgc ttgacatcct ttatggaaat atcttgcggc agtcttgcgt
     5041 tcaaggctgc ccgcaaagct tccggactgt gttgccaagc aagtctcgcc gaaaaaacct
     5101 gtccgtcggc atgaacgccg ctgtcagtcc ttccggcgct gataattcct gtttccttcc
     5161 aacccagcgc tgtcaaagct gtttcgagct ctgactggat cgttcttcgc cctttttgac
     5221 gttgaaagcc ccaataaagc gttccgtcat aagaaactgt aaatttgtaa cgttcacaag
     5281 tgtccggaac gggatttccc cgctccgaca actcactcac cgacaagttc gatgacaacc
     5341 atttccgcag cgtccccttt tcgagggccc agcttaatca tgcgggtata cccgccgttt
     5401 cgtttatcat accgtggggc aatatcgtca aacaactttt tggttaattc acgatcattg
     5461 cctaaccgag aagaggctaa gcgggcggca ttcatttttt ggattttatc gccctgattg
     5521 cctttttttg ccaatgtaat cagtttttca gcatcgttgc gcatcgataa agctttggct
     5581 tgtgttgtaa taattctttc atgctcaaac agctgagaaa tttgcgtcat acgcagtaac
     5641 ttccgctgag atgacgttct tcctaattta taacctgcaa ctccatgccg catttcaatt
     5701 actccgtatc actttctttt ttgagatagc ccttttcctg caatttctct cgtaattctt
     5761 caagactctt ctcgccgaaa ttacggattg atttcacagc atcatcccct ttgtccaata
     5821 aatcaagcac atctccgacg gttgcaacac cggtccgctt taatgaattg aagactctga
     5881 ccgacaggtc aaggctctca atcggtgttt cagcggtttc actcgtaaca tgtgtactgt
     5941 gttgttccgt tacgattgtg tccggcagta cctcttcggt tatgcccgaa atataacgga
     6001 gatgctcgat tagtattttg gaagctaaaa tcagagcggc ctgtggctga atggttccat
     6061 ccgtccagat ttccaactct aacttatcgt agttcgtact ctgtccgtaa agagcagagc
     6121 cgacattcca attgacgcgt ttaatcgggc tgaagatcgc atcaaccggc agctctccga
     6181 tggggagacg gtttgtcctg tcattcgccg gcaaataacc gcgtccgcgt tcaacggtca
     6241 tatcgatttc caaatgggtg tcggggttgt ccacagtgaa aagatataat tcaggattta
     6301 ggatttccac ttcagcaggg cattgaatat ccgaagcagt tacttccccc tctccattta
     6361 cttcgagacg gagatgtgag acatcgcctt catacatttt gaggcgaatc tgcttgattt
     6421 gaagcattac ctgtaaaacg tcctcgcgaa ctccgggaat tgtgctgaat tcatgcatca
     6481 catcagagat atggagggac gtaacagcca ccccttccaa tgatgagagc aaaacccgtc
     6541 gcaatgcatt ccccaaagtc acaccgtagc cacgttcgag agggtaaact acgaatttac
     6601 cgtagttctc agcttcacgt tcccgctcaa ctttcggtgt taccatattg cttaatgtag
     6661 ccaattttca cccctttccg atacaacaaa aaccaaatct gtatcggcgc ctaattcata
     6721 ttaccgtgag taaaactcaa cgatcaattg ttcgttaata ttcccgtcga tttcacttcg
     6781 ttccggcaga cgcagcacac gaccgctgag agctttcgtg ttccggtcca accataacgg
     6841 acaaacacgt ttgtcggcta tatcagccaa atcgctgaaa agcgcctttg aagcggaact
     6901 ttttcgaacc gaaatctgat cgccgggttt taagatcatc gaagggatat cgcttgggat
     6961 gccattaact tcaaaatgac cgtgtgtgac catttgacga gcttgagcac ggttttccgc
     7021 ataccctaac cggtatacga cattatctaa acgagtttcc aaaagctgaa gtaaatttaa
     7081 cccggttaaa ccggattgtc ttactgcaat gccgaatacc cggcggaact ggcgttccaa
     7141 aacgccatat tttcttcgta ctttttgctt cgcacgcagc tggcgggaat aatccgaaac
     7201 tctttctccg ccgcggctgc cgccggatct accgtgatca ccgggggcat aagcgcgttt
     7261 ttcaaaagaa cattttgcag aaaagcaacg ttcgccttta aggaataatt tttcattttc
     7321 gcgtcggcat aatttacata ccgacccagt atatcttgcc attgttcttt gttcctttcg
     7381 taattaaacg cggcgttttt tcggaggacg acaaccgtta tgcggtaccg gagtaatatc
     7441 cgaaatcgaa cgaactttca tccccatccc ttgaacggca cgaatcgcag attcgcgacc
     7501 cggacccggg cctttaatta tcaagtccac ttcctgaaca cccatggtct gagcggtctt
     7561 aatcgcttgt tcggcagcta atcgagcggc gaaaggtgtg ctctttcgag accccttata
     7621 accggaagca cccgaactgc tccagcatac cggattcccc atggcatccg tcacggtgac
     7681 gatggtgttg ttaaaagaag cgaaaatgtg cacctgagct gatcccagcg tgcgctttac
     7741 ttttcgtgcg gtagacgcac ggcgcgatga gcgtacattt tgagccataa atttcctcta
     7801 ccttaacctt atgtctgaaa agaatcaaat tatcgacagc aaacaattac aggcagccgc
     7861 agggagaaag gatcccaacc tgtaattatt tgccaaagcg aaattcgact tatttcttgg
     7921 ttgctccacg gcggcggcca cgtccggcaa cggtcttctt gggacctttt gcggttcggg
     7981 catttgtccg cgttctttgt cctcggcacg gtaaatttcg acgatgccga attccccggt
     8041 aacaaccaat ttccaccagg cgtttaatat tcatctgaac ttcgcggcgc aaatcacctt
     8101 cgacaacata gtttttggca atatactcac gaagcaaatt ctcatcattt tccgacaagt
     8161 cctttacccg aatgtccgga ttgacaccgg tatcccgcaa ggcttgttgg gctcgggtaa
     8221 gtccgatccc ataaatataa gtcaaaccta tttctatccg tttattacgc ggaagatcga
     8281 caccttcaat tctcgccata tcctcttatc cctgtcgctg tttgtgcttt gggttttcgc
     8341 aaataacgta caaacgacct gatcgtttga cgattttaca ctttgcacat ctttttctga
     8401 tcgatgaagt aaccttcatt ttaaactccc tcggatgttt ttccacccgg acttaacatt
     8461 aatagccaga caattatact cgtcatgact ttgtctgtca aagataatca tatactcatt
     8521 acagtttttt tcgtcccgtt accgacaccg gcggttcacc gttttccaac agcgtcaaaa
     8581 tcatcggacc ttcttccgta accgctatgc tatgctcaaa atgagcggtc aggctcaagt
     8641 ccgccgaaga aacagcccat ccgtcctgat gaacttgtgt cgcatcggta ccgaccaaaa
     8701 ccatcggctc aattgcgatg gttattcccg gtcgcaatcg aattccttga cctttggtcc
     8761 cgtaattcgg aacttgcgga gcctcccaca ttcgtcgccc gacgccgtgt ccggtgtatt
     8821 gtttggtcac ataaaatccg tgactttcaa catattcctg aatcgctgcg gaaacatcgc
     8881 ccgtgtaatt tccgggtacc attttttcga tcccgatata taaggaattt tccgttactt
     8941 ccatcagctt ctgcgcctct tccgagactt ttccgacacc gatcgtaaaa gccgaatcag
     9001 ccacgaaacc tttgtaggtt gtcccgcaat ccacggaaac aatatctccc tcatgcaaaa
     9061 cgattttctt gctcgggatt ccgtggacca gaacatcgtt tacactgaca caagtgcttg
     9121 ccggaaacgg aatcggaccg ggaactccct taaaaggaga agttactttg taatgatcca
     9181 gtactctgtc ggctgctgca ttcacatccc aggttgtcgc acccggaaca caaacctcgc
     9241 tcgcggcttt taaagcttcg gcattaataa tgcccgcttc ccgcataatc gcgatttcgt
     9301 cttttgattt gatactgact tgcctctccc agctcattct atcagcttcc cggtcatttc
     9361 ttggttacgt ctttgaatgc cgcaagaata tccatagtaa cggcatcaat atcctttgcc
     9421 ccgtctataa accgcaatac attccgcttt tgataataat cgatcaacgg tgccgtctga
     9481 ttttcataaa cttcaagtct tttttcgatg gtttccgctt tatcatcctc acgctggtaa
     9541 agtttttgac cgcaaatatc gcagatatca tcctctttgg gaggatgata taccatatga
     9601 aagacgtggc cattcggaca cattcggcga ccgcttaacc ggtcgatcag cagttcccga
     9661 tcgacttgaa tacatgggac gaaatcaact tcggtctgat aagcttcttt gagcatttga
     9721 tcaaaagctt cggcttgtgc ggaagttcgc ggaaatccgt ctaatattgc gccattaaca
     9781 caatccggtc gggatattcg cgatttgacc atctcaatcg tcacatcatc cggaacaagt
     9841 tgtcccgcat ccatgtagta ctgtgctttt ttcccgagct ccgtttgatt cttcaggttc
     9901 tctctgaaaa gatcaccggt actgatatga gggatcttca actcttcggc aagcctttca
     9961 gcctgagttc cttttcccac tcccggagct cccaaaaaga caatatatgt cgccattctt
    10021 tttctcttat ctcgctgttt cagcggatca atttttcttc gtagccatgt tggctcagtt
    10081 cactttgcag gtaatcgata atgtctctta cgacaccgac cacgatcagc ataccggaag
    10141 aggacatcaa catcgtcgct tgtgcactgg tatgcagtat gacagccaaa atattcggca
    10201 aaactgcgat aacacccagc atcacggcac cgggcaaagt gattcggttt tggatcttcg
    10261 ccagatattt ctgagtttcc gttccggctg ctctgccggg gactaccgcc ccttgtttct
    10321 tcagactgga gccgtaatca ttgttcatga aagtcacgct tgtataaaaa taagtgaacg
    10381 cgacaaccag cacaaaatat aaaacaacat agaggctgct tgaaccgctg aggtgactct
    10441 gaatccatac ggaagaattt ttcacccatg ccgtaccgga attcacaaag aaactggcaa
    10501 tcaatgccgg gaaagtaata atcgattgag caaaaatgag cggaatcatc cccacactat
    10561 tgacccgtaa cggtaacgtt gtcgacccgc cggatttaat accgcgatag ccgatccgtt
    10621 gaccggggat catcagtttc acatatcttg tcccctgctg gatataaacg atcgcaaata
    10681 ccgagatcag caaaactacc agagagataa ccaaaacgat ccagcccgtc gttttatccg
    10741 ccaaaattct aaccaggtta ttgggtatct gtgaaacgat accggagaag atgattaagg
    10801 acagaccttg ctgttggatg ccatattccg aaataatctg acccaaccaa atcgcaacca
    10861 tcgttcccgc aaccatagtg ctgaggttgg caagagtcgg cagaaagttg cttccgctcc
    10921 agccgtaatc ccgaagcacc gtcgtgccgg gaacgatttg gttaaagagg ctgatttggc
    10981 cgatagccga taaaatcgcc atcgggatgg tcagaatcat cgtccatttt tccatccatt
    11041 tttggccttc gcgcggatca tccttcattt ttcgctcgat cgccggaata atcgggccga
    11101 gcaattgaag gataatcgac gcggtaatgt acgggtaaac gcccattgcc ataatggaga
    11161 aagacgataa ggtaccgccg gataaaacat tcaggatact gatcaaatca cctgcggcag
    11221 ttgtgttgga cttcaatgct ttaataactt cggcattgaa ccccggcacc ggaatattcg
    11281 ctatcaaacg atagagtacg agtattccca acgtcaacat cagttttttg cgaatatctg
    11341 ttgacttcca cagaaaacgc catggggatc gcttcattct ttagttctcc ttaataactc
    11401 cagcgaacgg cttcagccaa aaacttcagc cgataatttc gacgctgccg ccggcagctt
    11461 caattttttc tcgggcacct tttgatatcc ggtggacctt gactttatat gcatgcgaga
    11521 cttctccgtt gcctaaaatg acaaccgggt tattcttctt actgagaaga ttcgcttcag
    11581 ccaatacttc gggagtaatc tcgatatcgc cgtcaaatcg ctcgagcatt tcgagattca
    11641 cttcattaaa ttcaacccga ttgggcggcg taaaaccttc cccacgcata aaaggcaaat
    11701 gacgataaaa aggtaaattt ccgccttggc ggtaaatatg accgccgtac ccttgacgga
    11761 cgtgctgtcc ttttgtacct ttgccggctg ttttaccctg tccggccgct aaaccgcgtc
    11821 cgaccctttt cttttgatgt tttgcgcctt cattcggtct taattcatcc agtttcataa
    11881 ttactctgcc aattcctcaa ccttgaccag gtgattaacc tttcgaagca tcccgcgaag
    11941 agaaggagta tcctctctta cgatcgaatc attcaagtgt ttaaacccca gtgccttgat
    12001 tgtatcctta tgtttccatg aatagccgat tgcacttttt acataggtaa tgcgcaattt
    12061 tttaggactc gaagtatctt tatccattct tcctccgttc ccagaaaggc agcaattcat
    12121 ccggttgttt ccccctcaaa atcgcttctt cctcggcatt ctttaattca ttgagcgctt
    12181 caaaagttgc tttgacaaca ttgagcagat tggaactgcc caatgatttc gtcaaaatat
    12241 catgtatccc ggcaacttcc aaaacagcgc gaacgccgcc tgcggaaata acgccggttc
    12301 cttcggatgc ggggcgtaat aatacctgcg caccgcctac gtgaccgata acctcatgcg
    12361 gaatggttgt tttatataaa tttattttcc gcatattctt tcgggcacgt tcggaagcct
    12421 tccgcatcgc atccggaacc gcattggctt ttccgattcc gagaccgatc gtcccgttgt
    12481 tatcgccgac gactacggtt acacgaaatt gaaagcggcg tccgcccttt actactttgg
    12541 cgacacgcgc gatttcgatc acacgctcat ctaattgttc atctaacggt tgataatcca
    12601 aattctccat ggttttagta cctttccttt tgcctaaaac tcaaggcctg cttcacgcgc
    12661 agcatctgcc aaggctttaa tacgacccat gtagcgaaat ccgccgcgat caaagacgac
    12721 tgtggtaatt cctttttcct ttgcgcgttc cgcaacggct ttaccaacca gcttagcctg
    12781 ttcggtttta ttcttcccat ccatatcggc tttcagtccg cgatcgatat tggatgcgga
    12841 taccagcgtt gtgccggtct catcattaat aatttgggca tagataccgt ttaggcttcg
    12901 gtaaacactt aaacgagggc gttgcgcgct tccggccaaa tccttacgga ctctggcatg
    12961 gcgccgaatt cttgcctcac ttcgagaaaa tttcgccata ttttattacc tctatttctt
    13021 agctttcccg gatttaccgg ccttacggcg gattttttca cccaggtaat gaattccttt
    13081 acctttataa ggttccggcg gtctgataaa ccggatttcc gcagcaactt gcccaacctg
    13141 ctcacgatca tagcccatga cttttatctg acgcgtcttt gtatcgacgt caaaagatat
    13201 tccttcaggt ggcacgacct ctttcggggc tgaataaccg acataaagca tcaggttttt
    13261 cccgttcatt tcggctcgat atccgacacc atccacttcg aggatgaccg taaatccctg
    13321 agaaacacct acgaccatat tatttaaaat tgcccgactc gttccgtgaa gagcgcgaat
    13381 agcagcttca tccgaggatc gtgtcacagt aagagttccg ttatcctgat caattttgac
    13441 aaccggagaa aagaggcgtt tcatctcccc tttcggccct tttactgtca cttcattccc
    13501 gttgatgtcc accttaacgc ctgccggaac ggtaatgggt aaacgtccaa ttcgagacat
    13561 ttcctttaat tcctttccta ccagaccttg cagaggattt cgccgcccac acccaactgg
    13621 cgggcgcgct gcccggtcat cactcccttc ggagtggaca taatcgctac acccaagccc
    13681 gacaaaaccc aggggatctc gtttcggccg gtgtagactc ttcttccggg gcgactgaca
    13741 cggacgatac cggacataac cggccggcgt gagcgtcgtt cacccgcata tttgattttc
    13801 aatttcagcg ctcgttgaga aggtttgtcg ccttcgagca cttcatagga ttccaaaaac
    13861 ccttcttctt tcagaatccg tgcgatttcg attttgatat ttgaacaggg cattacaacc
    13921 gtagcatgac cagccatcat tgcgttgcga atccgtgtca acatatcggc aataggatca
    13981 ttaaccatct ttctattcct ccgttatgga ttaccaggat gatttcgtaa cgccggtgat
    14041 ttggccgttc agtgctaatt cgcggaagca aatcctgcac aatccaaatc ggcgcatata
    14101 accccgcggt ctgccgcatc tcttacagcg attgcgaacc tgattggggt attcacgcct
    14161 gagttcacga tacatcatgc atttcttagc catattaatc cttcctgaaa ggcatgccga
    14221 gcaagtcaag cattacgcgc gcctgatcgt cattttgagc tgttgtaacg attgtgacct
    14281 ccatcccgcg gactttatcg actttttcat agtcgatttc ggggaacaga gtctgttctt
    14341 tcagccccaa tgtgtagttc ccgcgaccgt caaaagcatc cgtaggaaca ccgcgaaaat
    14401 cgcgcacacg aggcaaaaca atattgagca gccggtctaa aaagccccac attttttcac
    14461 cgcgtaaagt aactttcgcg ccgatttgac gaccttcacg cagcttaaaa tttgcgatgc
    14521 tgactcgggc tttggtgaca accggcttct gacccgtaat ctgcgccaga tcgttgaccg
    14581 cagcatccaa tgccttggga ttatccaaag cttcacccaa accgatattt acgactactt
    14641 tttcgatttt gggatactgc atcggattat ccaactttaa tgtagatttc atagcaggaa
    14701 gtacttcttg caagtatttt tctttcatgt gattcatgat ctatttgctc ccgctatttc
    14761 gcatcaattt cagaattgca ttttttgcaa attctggtaa ctttaccgtc ttcaccacgg
    14821 cttaacccaa ctcgggtcgg tttattgcag ttcggacaca ccaacatggc atttgaaatg
    14881 tggatcggtg cctcaaattt gacaattccc gcgtcaattt gacgacgtcc ggcacgtgtc
    14941 tgtccctgat gacgtgaacg gatattcact ccctgaacaa cgattcgcga ttcctttgga
    15001 atgacgcgaa ttacttcgcc acgtttcttt ttgtcttccg aacggccgct gataatttca
    15061 accgtatcgc ccttgcgaat ttttagtttc attcttcgct ccatctttcc aatgctcaga
    15121 tagtttccgg tgccagggaa acgatctttg taaagccctt ttcacgcaat tcacgcgcta
    15181 ccggcccaaa aatacgtgtc cctttcggat ccgtcccatt gacatccaga atcactgctg
    15241 cattatcatc aaaccggatc gttgagccgt cttcccgtcg ccattccttg gagcagcgaa
    15301 cgattacggc tttcacaata tcactctttt tgacagatcc gtgcggggat gcggacttta
    15361 ccgtaccgac gacaatatcg cccacatgac cataatgatg gacggaacca cccataatat
    15421 gaatcacaag gatttctttg gcgcctgtat tatcagtcac ttttaatctg ctttcctttt
    15481 gaatcattct ttggctcctt cgttcatcgc ttccgccaat tcggacgaga cttcctcgat
    15541 gatttctttc ttttcatccc gtcttatgac cgattgaacc acccatcgct tttctttcga
    15601 aagcggttgg ctttcaacaa tcatcacttt atctccgact ttacattcat tgttctcatc
    15661 atgtgctttg acatgtttca catcatggac aactttgcca accagcggat gacggtaaga
    15721 tcgccgaatt tcaacgacaa cggttttatc catcttgtca cttgtaacag tacccattat
    15781 tcgacggcgt gcattcatgc tttaccttcc tgttgtttct gcatttccat ttctcgcaga
    15841 atggtttcca tccgggcaat atcacggcga acttctttta cacgactaag atcacttaat
    15901 tggccggaaa cagcctgaaa acgaagattc atcttttctt gccttaaatc cgacagcttg
    15961 gctgtaattt catctttcgt taataaccga atttcagctg ctttcatgat tgttcttctc
    16021 ctgtgcccag ttcgtgtcgt gaaactactt tggtgcgaat ggaaaattta taggcagcct
    16081 ggtgcaatgc ttccaatgcc gcttcttctc ccaatccacc gatttcgaac ataattcgtc
    16141 ccggtttaac gactgcaacc cagtattcga cattcccttt tcctttcccc atacgggttt
    16201 cgggaggtcg atgtgtatac ggtttatccg gaaagattcg aatccaaacc tttccgtggc
    16261 gcttcatttc ccgcgccaat gtacggcgtg cagcttcgat ctgcctggcg gttacccagc
    16321 cgggttccag agccatcagc ccatattccc cgaaggtaat ttccgctccg cgcagagctt
    16381 ttccgcgcat tcgaccacgc tgctgcttgc ggtacttcac acgttttggc attaacatat
    16441 ttcaatacct ctttcaatta accccaacgt tcacggtgat cccgtaaacg tgatcagtat
    16501 tcgtcttatt cactgacgta aacaccttca gtggattcag cagcagcttc ttcgttcata
    16561 gaaagcacga taccgcgata aatccaaact ttgatgccga tttttccata ggtggtgttt
    16621 gcttcagctc gggcaaaatc gatgtccgcc cgcaaagtct gcaaaggcac acgcccttct
    16681 cgtaaggtca ctgtccgagc catatccgca ccgctcagac gaccgccgac ctgaatctta
    16741 acacctccag ctccttggcg taaaccttgc tgaatggcgc gcttcatcgc ccgacggtac
    16801 ccaacccggc gttcaatctg cccggcaata ttcatcgcga ccagataggc atccaaatcc
    16861 ggtgatttga tttccttgat ttccaaatca accttttttc cggtcagtgc ctcaagattc
    16921 tgacgaataa tttttacggt ttcgcctttt cgaccgatta agatccccgg tttggctgtg
    16981 ttcaccacaa ctttcacttt tcccgggaat cgttcgactt ccactttgga aacgcccgct
    17041 tttccggttt gttcgtgaat catgtttcgg atctgaagat cctgatgaag ctgatccaca
    17101 tattcatttc ctgtagcata ccaacgcccg tcccagggtt tgttgatatt caatcgaaat
    17161 ccgataggat gtactttacg acccatattg ttctcctgaa aactcctttg gtctcttcgt
    17221 tctcacacta ctcacgttca cgaagaacca cagtaacatg ggatgagcgg cgtaataccg
    17281 gtttgaaacg tccgcgcgct ccgaaatatc gccagcggcg tgtggaagct tcatcacagt
    17341 aaatcgaatg cacatacaga tcatttcgat ccaatccgaa attctcctct gcattggaag
    17401 cagctgaagc aatcaattta tagaccggtt cagcggatac tttattggta aacttcaaaa
    17461 tatttaaggc atccgtaacc gatttaccgc gtaccagatt gattaccaga cgaactttct
    17521 gggcggaaac accacaattg ttcagttttg ctataacatc ttgagccatg gtttttacgc
    17581 cttcgtcgct ttctccgaaa catgaccgcg gaataaccgg gttggggcaa attcgcccaa
    17641 acgatgaccc accatatttt cggttatata aacaggaata tggcggcgcc catcatgtac
    17701 ggcaattgta tgacctacca tctgtgggaa gatcgtgctt gcccggctcc atgttcgaat
    17761 tactttcttt tcgccggcgt cattcatttt ttcgaccttc ttcagaagtt tcgggtcaac
    17821 ataaggaccc ttctttaatg atcgtgacat aacttcgttc ctctctaaat ttactgaacc
    17881 gaggactaac gcccgctctt cgcgttgcga cgtcgcacaa tatatttatc tgttttctta
    17941 ttcgagcgag tcttatatcc gagcgccggt tttccccacg gggttttcgg cccgggcata
    18001 cccacaggtt gccgaccttc accgccgccg tgcggatgat cgcgcggaga catcgctgta
    18061 ccgcggacag tcggacgaat ccctaaatgt cgtttccgtc cggctttgcc gagtttaaca
    18121 ttgctgtgat ccaaattgcc caactgcccg attgttgcgt agcaggtttg cagaatcaga
    18181 cgaacctcac cggacggcag acgaatctgc gcataatcgc cttcttttgc cagtagttgc
    18241 gcggaagagc cggctgcccg aaccaattgg ccgcctttgc cgggtttcat ctcaatatta
    18301 tgaatggtgg aacccaccgg aatattggag atcggcatgg catttccggt gcggatttcg
    18361 actttcggtc cggagacaac ggaatcattc acctttaaac cgatcggtgc cagaatatag
    18421 cgtttctcgc cgtctttata ttgaacgagc gcgatccgag ccgttcgatt cggatcatac
    18481 tcaatcgcaa ttactttggc cggcacatca atcttatcgc gccgcatatc gacaatccga
    18541 atataacgtc gctctccgcc gccgcggtga cggaccgtaa tccggccgtc cgagttgcga
    18601 cctgcgtgat tttttcgaat cacgatcagc gatcgttccg gtttatcttt ggtgatttct
    18661 tcaaaggtat aacctgtcat accgcggaga ccgggggtga taggtttata aattttaact
    18721 gccattattc aactccttcg aagaacggaa tcttatcacc ggcagccagc gtcacaattg
    18781 cctttttcat gacaggcttc ttgagaaaca agcgacggtt tttggtattc cgtttggatt
    18841 ttccgggcgt tcgaatgata ttaacccgaa ccacattcac tttaaaaatg gattcaactg
    18901 cttctttcac catcgtccgg ttggcgtcag gatctacttc aaaaacatac tgattcagtt
    18961 tgaaagccat ttcatttgtt ttttcggtaa ttaagggacg gcgaaggatt tcaaaatctg
    19021 ttgtcatttt ctcctcccta tcctaaaaat gaattaattg tttcgatagc cgccaccgga
    19081 atgacgattc gatcgaaacg caacaaatca cgaatattta aataatttgc caacaagatc
    19141 tttgcataag gcagattatt ggttgcgcga atgacgtcga cgctggattc cttgtcggga
    19201 atcagaatca atgcgctgtt atcccccacc aggagagata atgcatttgc catcaggcgt
    19261 gtcttcggtt catcgagttt taactcatca accacaacga tttcggaatc gtttgctttc
    19321 acgcttaacg cagaccgcaa agccgctctt cgcatcttct ttggcatatc ctgagtgtaa
    19381 tcacgcatat gcggagtatg aacccgtccg ccgcctttcc actgagcagc gcggatggaa
    19441 ccttgtcgag cccgaccggt tcccttttgt ttatagggtt tcctgccgcc gccggcgact
    19501 tcatgccgta ccttggtttc atgtgttcca agacgagcat tggccatttg gcgaacatat
    19561 gcctgatgca tcaagtccat gtatatcggg gccgcaaaaa tcgtttccgg aagttcgatc
    19621 tggttgacga cttcgccctt catattcttg acatcgattt tcataatact tctcgctcca
    19681 tgttttcggt aaacccgtta agatttacgg gcatccctga tcataacaag accgccgcgt
    19741 gcgccgggaa cagcgccttt gacgccgatc agattccgtt ccgcgtcgat gaaagcgact
    19801 ttcaggtttt gtgccgtaac acgatcattg cccatatgac ccgggccacg ggaacccttg
    19861 aatacacggc ctggagtcgt tccggaaccg tgagaaccgg gagcgcgtaa acgatccgac
    19921 tgaccgtggg ttttcggtcc gccacggaag ttataccgtt tcacaccgcc ttgaaaacca
    19981 cgccctttac tggttccgac gacatcaacc agttcgccta aagcgaacaa atccacagta
    20041 actttgtctc cttcggagac ctgtggatct ttaacacgaa attcacgcaa aaagcgtagc
    20101 ggaggcaagt tgttccgctt taaatgaccc ttttcagcat tcgtgattcg tttcggttta
    20161 acctcttcga accccaactg caccgcgctg tacccatcgt tttcctgagt gcgcaactgg
    20221 gtaacatagc aaggcccagc ttcgatgacg gtgatcggaa ttgcaacacc attctcatcg
    20281 aaaatctggg tcatgccgat tttcttgccg agtagccctt taaacattct ctgttttctc
    20341 cccttttaag gttatatata atattcgaag gtttcgtaaa aaaccttttc tcaagattgt
    20401 cagcatgggc ttagctgcat catcttgtcc acagtcagtc aatccgcttc tgccacacca
    20461 gtggattttt gtcgtgcgca tacggtcgaa ttcttgacgt gcgatatcgg ttcgctttgg
    20521 catctatcaa agcaaactgc gacattatat cataagttta aaattttgtg gaatttgatg
    20581 tgaagtc
//
LOCUS       DAOSMM010000040        20489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2604_length_20489_cov_16.885779, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000040 DAOSMM010000000
VERSION     DAOSMM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 20489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20489
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_20489_cov_16.885779"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PK898_06895"
     CDS             complement(<1..321)
                     /locus_tag="PK898_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19797.1"
                     /translation="MTSVIQKNSLKSFLKKLVTQYSYWLSFFMLLILAVAVNTSFLRW
                     SNISNIFVQTATTGIIALGMTMIISAGQIDISVGAQVAIISGLGIVVLNRTNNILIMF
                     LFCMG"
     gene            complement(290..1819)
                     /locus_tag="PK898_06900"
     CDS             complement(290..1819)
                     /locus_tag="PK898_06900"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU19798.1"
                     /translation="MLVEMKGIVKTFGPVVAVDHVDFTVHPGEIRGLLGENGAGKTTL
                     MNVLAGTFPPTEGEIYIDGKKVQFSNSMQAIKMGIRFIHQEISLCNDLKVFENMFLAE
                     EILLPNGLLNKKEMIKRASEVFNRMQVNINPEELVWNLQPAEKQLVEIARALLFKSDL
                     IIMDEPSTALTTKDIERLFVIMRQLKSEGVSFIYISHKMPELFEVCDTFSVMRDGKLV
                     AEGNFSDVNEQEITELMIGRQLAEEEFSHKPCYRTDEIALSVRNLSGPGFQNISFDLH
                     RGEIIAITGLQGSGRDSLADALFGAIPSTGEIEIFGKKINPESGIIERMKSGIAMVPR
                     SRKERGILNDLSIYDNLYMAYLNTKFKGLLIQPKDQKERFKRQQESLSIKTDDPKNPI
                     TSLSGGNQQKIILGRWLEADAEIILFDNPTQGIDVGTKFEIYHLIINLAKQGKAIIVF
                     SSEFPEIFKVADSCIVLFKGNINARLSREEITERNIMYYSTGANLEAEKNDKCDSKKL
                     S"
     gene            complement(1903..2958)
                     /locus_tag="PK898_06905"
     CDS             complement(1903..2958)
                     /locus_tag="PK898_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU19799.1"
                     /translation="MSKKLFVLSVVFVLVLALTGSVLAADTFKIGVVTPDADHGFTGE
                     SVAHARAELEAKSKELGFEYKFEVGGEAAKQIAALETILEWKPDVIVLWPLEGQQLKN
                     IAMTIMDSGVKLIIYDRLIDGFKPTAEIMGDNESIGKMMGEYLLKYFDEQLKAGETIT
                     YLRFIGDSSTVSIQRSKGMDDVIAASAYKDQFKQIQENYQTDWSNAKAQEQMENWLNT
                     ADPKEIEDLDLIVTHDDEVVDGVVVALNNYYNSNPSAKLNVKLITGVGGRRETLATFD
                     KPVANGIDLVTYYFSPSFIREAIRLGIAAAKGEQYQGQDINGQLFLIPSIEIDKTNVD
                     EFRNSDAFKERYSISLN"
     gene            complement(3012..4043)
                     /locus_tag="PK898_06910"
     CDS             complement(3012..4043)
                     /locus_tag="PK898_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU19800.1"
                     /translation="MAVTIIDVAKEANVSASTVSRVLNNKSTISENTVRRVYEAAERL
                     GYEPNILAQSFRKQETRTILILTPNMTNPYYSNILSGISDTTRSLFYSSFICKTDWNR
                     EEMSNILGMMKKRHADGAILLACEMGEEDWLLRYAKDNPIILCSEFNPDTSIPHISID
                     NYKATVEAIEYLISLGHTKIGTISSVNKYLSTQLRLKGYKDALAAAGIEFHPEYVEYA
                     SKDYSYRSGCASARKLLSLEDRPTAIFCISDVLAIGAINTAAEMGIKVPEDLSVIGFD
                     DVEQTAMFSPKLTTVAQPCYELGKKSAELLITAFNQSEDVPLETVLPYQLILRESTGS
                     PVKIKEESI"
     gene            4299..8306
                     /locus_tag="PK898_06915"
     CDS             4299..8306
                     /locus_tag="PK898_06915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU19801.1"
                     /translation="MSFVHLHLHTEYSLLDGFTKINKLMTRVKEMNMPAVAITDHGTM
                     FGVVEFFFAAKEAGIKPIIGLEGYLSKGSMTSRDPKEKTSSHLLLLAKDMTGYKNLLQ
                     IASASQLEGYYYHPRIDHEYLAEHAEGLIATSGCMAAELPRMIINGESEAAKAQLDWY
                     LQVFGRENFYIELQDHDVPEIKKLNNVLLRLGKEYDIPFVATNDTHYVDPEDWKYQDI
                     MLAIQTGAKLTDSNRFRMSDKSYYLRSPEEMEKLFGYIPGAIENTLAIAERCNVDLSS
                     KGHHLPIFPLETGETAESELRKLCEIGLKERYGDRVDREPVIRERMEKELGIIHQMGF
                     DAYFLIVQDLTRHAREENIWYNVRGSGAGSMVAYVLGITPVDPLKFKLIFERFLNPER
                     ISMPDIDLDIQDDQRYKMLQYCSDKYGADRVSQIITFNTLGAKGAIRDVGRVMDIPLS
                     EVDRICKLIPAGIKMPVSGKSITLNNCLEEIPEFSDAVRADPRLTELVTTASEMEGVT
                     RNVGTHAAGVIITDVPIVEYAPLHRPTSGSDDNPIKSVAQFEMNYVDKMGLLKIDFLG
                     LATLTVMQKCCAMIEKRHGIHLDLQNIPTDDKKTFAYLSQGHTAGVFQLEGSGMTRYI
                     KEMKPKSLDNIIAMVALYRPGPMQFIPDYIACMHGEKEPFYRHERLKPIFAETYGIPV
                     YQEQIMSAVMELAGYTAAESDSFRKAISKKKKAEIEEHREKFVSGCVKNGIEKTTAEE
                     IFKDWEQFANYGFNKSHAADYGVLAVQTGYLKAHYTVEYMAAILDANRNDSAKIAFYI
                     NDCRSIGIEVLPPEINVSDWDFTIEEDSNHKSRIRFGLGAIKNVGKNSVDPIIDERKQ
                     NGKFTDLNNFIERVDLRQVGRRALESLIKVGAMDAFGERGALLASMDMMINASSTNFK
                     SKDSAQIDLFDLMNIPTSQITLPQTPPIENRQKLIWERELLGLFVSDHPLNAHRKILK
                     ARKALQINQLEAMENGAIVAVGGFVQSMRMMITKKDSRSMCSIKFEDINGDSIDVIFY
                     PTVWDQVQDAVKTDAILIIEGRLDKSRSDLQISGMRVENLVVNGISKQLADYVDQFTD
                     ETVAETEINDDFVEERDSSEQNPSFSAELTSSETKDEKDFALEECPDPFFESYTAQPV
                     PLIREDAPYSVTEQVSDSVKIPESEKACAVLPAEEFSSDHRIEDEMIPWEEVHEESSV
                     ANENAEKDLSAEQPTTFGINANSDKPYEDLKETEDDEEDIFLEEEKPKPRLLTLIFES
                     DPKINPDVLVRRMQRIHGMVTAFPGNDKFAFLIREFDQVHLVEFPDSDTKICNELINA
                     LSQELGEKNVRVEEG"
     gene            8401..9384
                     /gene="pfkA"
                     /locus_tag="PK898_06920"
     CDS             8401..9384
                     /gene="pfkA"
                     /locus_tag="PK898_06920"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOU19802.1"
                     /translation="MEPETPIRSIAVLTSGGDAPGMNAAIRAVVRAGIYKGLKVYGVQ
                     DGFKGLINGDFVPLGRRDVSGILSHGGTFLRTDRSSEMKTPYGQREAVRQLNNEGIDG
                     LIVIGGDGSMAGAKALCDEGTRVIGIPGTIDNDVWGTDSCVGVDTALNTIMEAVDKIR
                     DTAASHTRAFLVQTMGRECGYLAVQSAMISGAEMALIPEDPVSVEEIAQTVENAYKRG
                     KRHCIIIMAEGYPIPMVDLAKQLDEMDLGFTTRSINLSYVQRGGSPTSFDRILASRMG
                     YAAVELISSGQTSLMTAMQGREIIGLPLIDIVGKHRPISEEYLHISNILSR"
     gene            9609..10883
                     /locus_tag="PK898_06925"
     CDS             9609..10883
                     /locus_tag="PK898_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19803.1"
                     /translation="MKKVYLLVVFVLLALLVVPVAAQEAEPGAKLVIESWRQDDIPIW
                     NDVIIPAFNKIHPEIQVIFQPTKQEDYNGQLNTRLAGGTAGDLITCRPFDPSLKLYES
                     GYLANLNDLPGMQFFDSSAKVAWTTDDGSVTYCVPMASVIHGFMYNKTAFEQKGWKVP
                     VTKDEFWALLDAIKADGEYIPLAMGTHDLWEAATMGWYNIGVQNYKGEEGRLAIINGT
                     AKFTDPPYVKTYEELAKWAPYLPEGYQSVQYSDAQQLFTMGRAAIYPTGSWEISVFEP
                     MINGAFEMGAFRVPKDNADDPCYISDHTDIGIGMNAATKYPEAVKIFLNWVATPEFAT
                     LYANNMVGFYPLAKIDYTIDNPVAQEFLSWRKDCQSSIRISASIIGRGEPGMDTDIWQ
                     TSANVINGTMTPMEAAQYVQDALAKWYKPQQK"
     gene            11149..12081
                     /locus_tag="PK898_06930"
     CDS             11149..12081
                     /locus_tag="PK898_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU19804.1"
                     /translation="MNATRKVRKPFPWHLVVFIAPALIIYVIVMVIPLLDSLTLSFFT
                     EDPNNARNTIFVGIQNYKYLFTDKYYAPIFWGAFKNNIVFFLIHMLVQNPIGLLLASV
                     LCKGGKIRNFFRTVLFIPTILSFVLVGFVWKLILSPVWGVSKTILNIFGLGGLFRPWL
                     GLEETALITCSLISVWQYIGTPMILFYTALIAIPDELTEAAYVDGANAWQIFWRVKFP
                     LILPTVGLVAMMTFVGNFNAFDLIYTMQGALAGPNLSTDIMGTLFYRTFFGQQQQMPN
                     QTLGATIAAMQFIIILIGVVIYMIWQKRTTTYEY"
     gene            12092..12970
                     /locus_tag="PK898_06935"
     CDS             12092..12970
                     /locus_tag="PK898_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU19805.1"
                     /translation="MTATELNKKKGASVSYRIISWLRSAGPILFLIFWSIIALFPIYI
                     ILVNAFKNRNAIFKYPFNIPNAETFSLIGFETVNKRANFARYFTNSIVVTVVSLAITL
                     LIATMASFALSRYKFKGATLLSLIFSIGIMIPIRLATVGILRLMVNWSLTNTLLSLII
                     VYIAQSLPMAIFILSSFMKQIPQELIDAARVDGASEYRIYWMILPLIRPALGAIGIFS
                     MIPIWNDLWWPLILAPSAETATVTLGAQQFLGQYMTDWNAVLASLTMSIIPILIFYLI
                     FSKQMIKSITSGAIKS"
     gene            12998..13336
                     /locus_tag="PK898_06940"
                     /pseudo
     CDS             12998..13336
                     /locus_tag="PK898_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            13391..14008
                     /locus_tag="PK898_06945"
     CDS             13391..14008
                     /locus_tag="PK898_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU19806.1"
                     /translation="MVDSGQIGKPGTIHLRRCSYRPKKPSNNWFLDEQKSGGILMDLA
                     IHDFDYARWVAGDVRTVYTQKVSSLDPSSPIDFGLTILTHENGTLTHVTCGWAYPPPN
                     FQTGFDINCERGAVHFDSEETMPIHNLIQTDKDAPDVALPGSPLAESPYTSEIKEFYR
                     AICYGDEVRVTGMDGVKAVQIACAARESARTGEPVQIKPLEGVLK"
     gene            14005..15000
                     /locus_tag="PK898_06950"
     CDS             14005..15000
                     /locus_tag="PK898_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU19807.1"
                     /translation="MIKIGIMSFAHLHAEAYIQNIRSIPGVEWIGFVDPDSERVKQFS
                     EQFRTWGTSDFDEFFAKKPDGVVICSENNNHLSMVETAIAAGVKNICCEKPLATTLED
                     AEKIVSLTESKKINLMTAFPMRFSAPIVAVKDQLDRGVYGKIYCFRSENEGQMPSLHR
                     KWFVDKKLAGGGALQDHLVHLTDLFRWITKSEVDSVYAQSSQCFHRGEVDVETAGMAM
                     LHFKNDCFATIDCSWSRPDYYPTWGGLNFEVVTDRGAVQVDAFRQNLSVYRHDIQNQV
                     WHYWGSDSNQGMIEEFAASIREERPACITATDGLRAVEVVQAAYESAASGKTVKL"
     gene            15168..15845
                     /locus_tag="PK898_06955"
     CDS             15168..15845
                     /locus_tag="PK898_06955"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOU19808.1"
                     /translation="MDVKITWLGHSAFLIDFDHYKVLLDPFLTGNSNAAIPAKKAEAD
                     LILVSHGHSDHVGDAIEISNRCHAPILCNFELSNWFAKHGAVNTMPIYVSGTRAFPFG
                     TVRATIAVHGSGTPDGTYGGLALGFILATHDQQKKIYFAGDTGVFSEMSLIGEDAVDV
                     ALLPIGDIFVMGLSESIRALKMIQPKNVIPMHYDPGLNLEGWKREVIETSGAKPIVLR
                     SGESFDL"
     gene            complement(15870..17921)
                     /gene="uvrB"
                     /locus_tag="PK898_06960"
     CDS             complement(15870..17921)
                     /gene="uvrB"
                     /locus_tag="PK898_06960"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOU19809.1"
                     /translation="MRNKFELTAPYQPMGDQPEAIKALVDGVQKGYRHQVLLGATGTG
                     KTFTMANVIQQLQMPAVVMAHNKTLAAQLYAEFKEFFPKNAVEYFVSYYDYYQPEAYV
                     PRHDLYIEKEVDTNDEIDRLRLAATAALASRSDVIIVASVSCIYGLGDPEAYGKTVIN
                     LKVGTITRRNALLRQLVESQYQRNDMDFHSGTFRVRGDTLEIFPAYEDSVAYRISFWG
                     DEIEKITEVNPLTGELVTTLDEIAIYPAKHYITEESRIKQAIVDIETELEERIKYFKD
                     NNRLLEAQRIEQRTKFDIEMIKEIGYCSGIENYSRHLDQRPAGSHPWTLMDYLPSEYL
                     LFIDESHMTIPQIRGMYHGDRSRKSVLAEYGFRLPSALDNRPLTFEEFEKTMGYTIYT
                     SATPGPYELEHSQQIAEQIIRPTGLVDPQVEVRPTKGQIDDLIGEIRKRTAANQRVLV
                     TTLTKRMAEDLSEYLKDNDIKVHYLHSEVDTLERISILRDMRTGVYDVIVGINLLREG
                     LDLPEVSLVAILDADKEGFLRSGVSLIQTIGRAARNVDGKVILYADKMTDSMKYAIDE
                     TNRRRKKQQAYNEAHHITPMTIVKGIYDLSERLTVSNAIAEEKADYKTTRKNLPKNEL
                     KHILADLEREMKEAAKNLDFERAAELRDQVYELRGLVADVSDASPWQRAKMLAGEIDL
                     K"
     gene            complement(18169..18693)
                     /locus_tag="PK898_06965"
     CDS             complement(18169..18693)
                     /locus_tag="PK898_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU19810.1"
                     /translation="METWLDSVNIRLMRESDLPLIEWGDEYVRYRKIYREVYRNFLAG
                     RAFPWIAEIEKYGVIGQVFLTEKQPDPAFDPEQPYMFLSSFRVKPDFRNRGLGTLLLK
                     ICEVTARDRNIHNVFLNCAKTNKRCKAFYERNGFTVLREDPGKWSFIDHKGAVREEAE
                     PAWTMVKKIETGDD"
     gene            complement(18729..>20489)
                     /locus_tag="PK898_06970"
     CDS             complement(18729..>20489)
                     /locus_tag="PK898_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU19811.1"
                     /translation="IMSTRPLLIDSQMPMEEIAELIQRYGYEGYPVVDKNKNLIGLLN
                     RRSVDRALAFGLSPSVESIMDPGNYSVHPEDSVDYLKEMMNLSGWGQIPVINGDGHEI
                     IGIVTRTDLIKILTGRSSVPGNRNYAQILTRVLPPSYLTLIHMVADTAWNCRYPVYIV
                     GGFVRDLILGQPVMDFDIVVEGDAIHLARTLVHIYGGKVRIHSRFGTAKWVIEDIHDQ
                     IIKNTPPSAEGAPESDLPATLDLISARTEFYDYPTAMPKVERSSIKLDLHRRDFTINT
                     MALRLDGDYFGDLLDYWGGYLDLHQKLIRVLHSLSFMDDPTRMLRAVRFEKRFNFTIE
                     KHTMMLLKDGKTLLKRISGRRIMNELDLFFHEESPSAGLSRLEELGLLEQIHPNIRWT
                     SQNTANLTKFSQKDPPDYWLEALPSLPEFINLEGKYLIWWGKNSPETIKEIVQRLQLS
                     QRIQKMLLSYSEICQKMPDLVHSWPSEVTFFLEKQSLEAVYCYDILSDDEKVHETIRN
                     FLKTWRNMKPFTTGAELRRLPYPAGSWIGAVLDRLRKGWIDEEISSREEEEKTLEFIL
                     PLFTKSLDKKHSSGNPAAEI"
BASE COUNT         5506 a         4436 c         4869 g         5678 t
ORIGIN      
        1 tcccatacaa aacaagaaca tgataagaat gttgttggtc ctgtttaaaa cgacgatgcc
       61 caaaccggag ataatcgcaa cctgagcgcc tacggagata tcaatctggc cggcagaaat
      121 aatcattgtc attcccaatg caatgattcc ggttgtcgct gtttgaacga aaatgttgct
      181 gatattgctc catcgcagga agctggtatt taccgcgacc gccagaatca agagcatgaa
      241 gaaagacaac cagtagctgt attgggtaac gagtttcttc aggaaagatt taagagagtt
      301 tttttgaatc acacttgtca tttttttctg cctccaaatt tgctccggtt gaatagtaca
      361 taatatttct ttcggttatt tcttcacgtg aaaggcgggc gttgatattt cctttaaaaa
      421 gaacgataca ggaatcggcc actttgaaaa tctcgggaaa ctccgaactg aatacgataa
      481 ttgctttccc ctgtttcgcc aaattgataa tcaggtgata tatttcgaat tttgtcccga
      541 catcgattcc ttgcgtcgga ttatcaaaaa ggatgatttc tgcatcggct tccaaccaac
      601 gtccgagaat tattttttgt tgatttcctc ccgaaagaga ggttatcgga tttttgggat
      661 catccgtttt aatggataat gattcctgtt ggcgtttgaa ccgttctttt tggtctttgg
      721 gttggattaa taaaccttta aatttggtgt ttagataagc catgtaaagg ttatcgtaaa
      781 tggaaagatc atttaaaata ccccgttctt ttcgggaacg cggcaccatg gctatgcccg
      841 acttcattcg ctcgatgatt ccgctttccg gatttatttt tttgccgaag atctcgattt
      901 ctcctgtaga agggattgcg ccaaaaagtg catcggccag agaatcccgt ccggatcctt
      961 gcagacctgt gatagcgata atttctcctc tatgcaaatc gaaactgata ttttgaaagc
     1021 ccggaccgga aagatttcga actgaaaggg caatttcgtc cgttcgataa caaggtttat
     1081 gggagaattc ttcttcggcg agttgtcggc cgatcatcaa ttcggtgatc tcctgctcat
     1141 ttacatccga aaaattcccc tccgcgacca attttccgtc ccgcatgacc gaaaaagtat
     1201 cacatacttc aaacaactcc ggcattttat gcgaaatata aataaaactg acgccttccg
     1261 atttcaactg gcgcataatg acaaacagcc gttcgatatc cttggtcgtc aaagccgtag
     1321 acggctcatc cataataatc aggtcactct taaacaacaa agcacgggcg atttcaacca
     1381 gttgcttttc ggcaggttgc aagttccaaa caagttcttc cggattgata ttaacctgca
     1441 ttcggttaaa aacttcggat gcccttttga tcatttcctt cttatttaag agaccgttcg
     1501 gcagaagaat ttcttccgcc aaaaacatat tttcaaaaac tttcaaatcg ttacagaggc
     1561 ttatttcctg gtgaataaaa cgaatcccca tcttgatcgc ctgcatcgaa ttggagaatt
     1621 ggactttttt cccgtcaata taaatctccc cttcggtcgg cggaaaagtt cccgccagca
     1681 cattcatcag agtcgtttta ccggcgccgt tttcacccaa caagcctcgt atttcccccg
     1741 gatggactgt aaaatccaca tggtcgactg cgaccaccgg tccgaaagtt tttacgattc
     1801 ctttcatttc tacgagcaag gcaggttctc cttatacttt attgaaaaac tgccggtcta
     1861 aaaaaaccgg accggcagta tacacttgaa aagataaaat ggttagttga gtgaaatgct
     1921 atagcgttcc ttgaaagcgt cactattgcg gaactcgtcg acattggtct tgtcaatttc
     1981 aatggacggg ataaggaaga gctgcccatt tatatcttgt ccctgatatt gttcgccttt
     2041 agcggcggca atacctaagc ggatggcttc acgaatgaag gacgggctga aataataggt
     2101 taccagatcg ataccgtttg caaccggttt atcgaaggtt gccagtgttt ctcgtctgcc
     2161 gcctacaccc gtgatgagtt tcacgttcag ttttgccgaa ggattgctgt tatagtagtt
     2221 attgagggca actacgacac cgtcgacaac ctcatcatca tgggttacga tcagatccag
     2281 atcttcgatt tccttcggat cagctgtgtt cagccagttt tccatctgtt cctgtgcttt
     2341 tgcgtttgac cagtcggtct gatagttttc ctgaatctgc ttgaattgat ctttgtatgc
     2401 ggatgcggca atcacatcat ccataccctt cgatcgctgg atggaaacgg tcgagctgtc
     2461 accgataaag cgcaggtagg tgattgtttc gccggctttg agttgttcat cgaaatactt
     2521 gagcaaatat tcgcccatca tttttccgat actctcgttg tcgcccataa tttcggcggt
     2581 cggtttgaaa ccgtcaatca gacgatcata gatgatcagt ttcacaccgg aatccataat
     2641 ggtcatggca atgttcttta attgttgacc ttccaacggc caaaggacga tgacatccgg
     2701 tttccactca agaatggtct cgagagcggc aatctgttta gctgcttcgc cgccgacttc
     2761 aaatttatat tcgaagccga gttctttgga ctttgcttcg agttctgcac gggcatgagc
     2821 aacgctctcg ccggtgaaac catgatccgc gtccggagtg actacaccga tcttgaatgt
     2881 gtcagctgcc aggacggaac ctgttagtgc aagcactaag acgaaaacaa cggataacac
     2941 aaaaagcttt ttagacattt tttccctcct aaaagattta ctgcgttttt gtgaacgatt
     3001 tgaagtgctt ttcaaatcga ttcctcctta atttttacgg gacttccggt cgattctctt
     3061 aggattaatt gatacgggag aaccgtttca agcggaacgt cctcggactg attgaatgcc
     3121 gtgatcagca attcagccga ttttttcccc aattcataac atggttgagc gactgtggtt
     3181 aatttcgggc tgaacatcgc cgtttgctcg acgtcatcga acccgataac cgataaatct
     3241 tccggtactt tgatacccat ttcggcggca gtattgatgg caccgattgc cagaacatcc
     3301 gaaatgcaga atatagcggt cggccgatct tccaatgaca gcagctttct ggcagaagcg
     3361 caaccgcttc tgtaactgta atccttggat gcatattcga catattcggg atggaactca
     3421 attccggcgg ctgccaaagc atctttgtat cctttcaagc gcagttgagt ggacaaatat
     3481 ttgttgacgc tgctgatcgt tcctattttt gtatgaccga gcgaaatcag atactcgatg
     3541 gcttccaccg tcgctttgta attgtcgatg gaaatatgag gaatactggt atccgggtta
     3601 aattccgaac agagaataat cggattatct ttcgcatagc gcaaaagcca atcctcttcc
     3661 cccatttcgc atgccaataa aatagcaccg tctgcgtgcc gtttcttcat cattccgagg
     3721 atattactca tttcttcgcg attccaatcg gttttgcaga taaacgagct gtaaaaaagg
     3781 gatcgtgtcg tatcgctgat gcccgataga atattggaat aataaggatt ggtcatattg
     3841 ggggttaaaa tcagaattgt tctcgtttcc tgtttgcgaa aagattgtgc cagaatattc
     3901 ggttcatatc ccaacctttc ggcggcttcg tagactcgtc tgaccgtatt ttcgctgatg
     3961 gtgcttttat tattaagcac ccgcgagaca gtcgaagcgg acacgttcgc ttccttcgct
     4021 acatcgatta tggtaactgc cattttattc ccgttgttca taaccctttt gctatctgtg
     4081 caatcgtttg cataaattat tctatatact ccggcggcat ttgtcaattg ccaaaatgtc
     4141 atcatttgaa acatgacgaa agtcataaaa taagtagatc gaattaattg aaaaacaaga
     4201 cggaattttt ctgaattcgg cggaatataa aaaaagcaaa agcggatgcc cgaagaaaaa
     4261 tatactaaaa tatgaatagc tttggagcat ttaaaactat gtcatttgtt catcttcatc
     4321 ttcataccga atattccctt ttggacgggt tcacgaaaat caacaaactg atgacccgtg
     4381 tcaaagaaat gaatatgcct gccgtcgcaa tcacagacca cggaacgatg ttcggagtcg
     4441 tggaattttt cttcgcggca aaggaggcgg gtattaaacc gatcatcggg ctggaaggat
     4501 atctttccaa aggatcgatg acctctcgtg atcccaagga aaaaacttct tcccatttgc
     4561 tgttgttggc aaaagacatg accggataca agaatttgtt gcagatcgcg agtgcctccc
     4621 aattggaggg atattattat catccgcgca tcgatcatga atatcttgcg gaacatgcgg
     4681 aagggttaat cgcgacttcg ggatgtatgg cggcggaact gccgcggatg atcataaacg
     4741 gagagagtga agcggctaaa gcgcaattgg attggtatct tcaggtattc ggacgagaaa
     4801 atttttatat cgaattgcag gatcacgatg tcccggaaat aaaaaaactg aataatgttt
     4861 tgttacgtct gggaaaggaa tatgatatcc cgtttgtggc gacaaatgac acccattatg
     4921 tcgatcccga agattggaaa tatcaggata ttatgctcgc cattcaaacc ggtgcgaaac
     4981 tgacggatag caaccggttc agaatgtcgg ataagtccta ttatctgcgt tctccggaag
     5041 aaatggagaa gctgttcgga tatatcccgg gtgcgattga aaacacattg gcgattgcgg
     5101 aacgctgtaa tgtcgatttg agcagcaaag ggcatcatct gccgattttt ccgttggaaa
     5161 cgggtgaaac cgccgaatcc gagttgcgga aattatgtga aatcggcttg aaagaaagat
     5221 acggtgatcg tgtcgatcgg gaaccggtca ttcgtgaacg gatggaaaag gaactcggca
     5281 ttattcatca aatgggattc gatgcctatt ttttgattgt tcaggatttg acgcggcatg
     5341 cccgagagga gaatatttgg tataacgttc gcggttccgg agccggttcg atggtagctt
     5401 atgtattggg aattacaccg gtcgatccgt tgaaatttaa gttgattttc gaacgatttt
     5461 tgaatccgga aagaatttcg atgcccgata tcgatctgga tattcaggat gatcaacgat
     5521 ataaaatgct gcaatattgt tcggataagt acggtgccga tcgggtttca cagattatta
     5581 ctttcaacac actgggtgcg aaaggtgcca tacgcgatgt cgggcgggtg atggatatcc
     5641 ccttgtcgga agtagaccgt atctgcaaac tgattccggc gggaattaaa atgccggtga
     5701 gcggaaaatc cattacgttg aataattgtc tggaagaaat tcccgaattc agcgatgcgg
     5761 tgcgggccga tcccaggctt acggagttgg taacgacggc ttccgagatg gaaggtgtga
     5821 cgcgcaatgt cggcacacat gcggcgggtg taatcattac cgatgtaccc atcgtcgaat
     5881 atgcccctct tcatcgaccg acatccggat cggatgataa cccgataaaa tctgtagctc
     5941 aattcgaaat gaattatgtc gacaaaatgg ggttgctgaa gattgacttt ttagggctgg
     6001 caacgctcac ggtgatgcag aaatgctgtg ccatgatcga aaaacgacac gggattcatc
     6061 tggatctgca gaacattccg accgacgata aaaaaacttt tgcatatttg agccaggggc
     6121 acaccgccgg ggtcttccaa ctggaaggtt cgggtatgac ccggtatatc aaagagatga
     6181 aaccgaaatc tctggataac atcattgcga tggtagcgct ttatcgcccg ggaccgatgc
     6241 agtttattcc ggattatatc gcctgtatgc acggtgaaaa ggaacccttt tatcggcatg
     6301 aacggttgaa accgattttt gcggaaacgt acgggattcc ggtatatcaa gaacagatta
     6361 tgtctgcggt gatggaattg gcaggctata ccgctgccga gtcggacagt tttcgcaaag
     6421 ccatttccaa gaagaaaaag gctgaaatcg aagagcatcg agagaaattt gtttccggct
     6481 gcgttaaaaa cgggattgaa aaaacaaccg ccgaagagat tttcaaagat tgggaacaat
     6541 ttgcgaatta cggtttcaac aaaagtcatg cggccgatta cggagtttta gccgttcaaa
     6601 cggggtatct taaagcgcat tataccgttg aatacatggc tgcaattttg gatgccaacc
     6661 gaaatgattc cgcaaagatt gccttttata tcaacgattg ccgatcgatc ggaatcgagg
     6721 tattgccgcc ggaaatcaac gtcagcgact gggatttcac aatcgaggag gattcgaatc
     6781 ataagagcag gattcgattc ggactcggtg ccatcaaaaa tgtcggtaaa aattccgttg
     6841 atccgattat cgatgaacgt aagcaaaacg gaaaatttac cgatctgaac aacttcatcg
     6901 aacgggtcga tctcaggcag gttggcagac gcgcactgga aagcttgata aaagtcggtg
     6961 cgatggatgc tttcggagaa cgcggcgcct tattggcaag tatggatatg atgatcaatg
     7021 ccagctccac aaactttaaa tcaaaggaca gtgctcagat tgatctgttt gatttgatga
     7081 atattccgac atcccaaatt acgctccctc agacacctcc gattgaaaac cggcaaaaac
     7141 taatttggga gcgtgaattg ctgggattgt tcgtttcgga ccatcctttg aatgctcatc
     7201 gaaagatttt aaaagctcga aaagctttac aaatcaacca attggaagcg atggagaacg
     7261 gcgccatagt tgcggtaggc ggatttgttc aaagtatgcg aatgatgatt acaaaaaagg
     7321 acagccggtc gatgtgttcg atcaaatttg aggatattaa cggagacagt atcgatgtca
     7381 ttttttatcc gactgtttgg gatcaggttc aggatgccgt aaaaacagac gcgattttga
     7441 ttatcgaagg ccggctggat aaatcccgtt cggatttgca aatcagcggg atgcgagtcg
     7501 aaaacttggt tgtcaacggt atttcaaaac aactcgcgga ttatgttgat caatttacgg
     7561 atgagacggt tgcggaaacc gaaataaacg atgactttgt cgaagaaaga gattcgagcg
     7621 aacaaaatcc ttctttttcc gcagaattaa cttcttccga gacgaaagat gagaaagatt
     7681 tcgcgttgga agaatgcccc gatccttttt ttgaatccta cacggctcaa cctgttccgt
     7741 tgattcgcga agatgctcct tattcggtca cggaacaagt atccgattcc gtaaaaattc
     7801 cggaatcgga aaaagcctgt gccgttttac ccgctgaaga attttcttcc gaccatcgca
     7861 ttgaagatga aatgattccg tgggaagagg ttcatgaaga gagttctgtg gcgaatgaaa
     7921 atgcggagaa agacctgtct gcggaacagc cgacaacctt cggtataaat gcgaattccg
     7981 ataagccgta tgaagaccta aaagagacgg aggatgacga agaggatatc ttccttgaag
     8041 aagagaaacc caaaccgcgg ctgctcactt tgatttttga atccgatccg aaaatcaacc
     8101 cggatgtatt ggttcgccgg atgcagcgga tccacgggat ggtgacggca tttcctggaa
     8161 acgataagtt tgctttttta attcgtgaat tcgatcaggt tcatttggtc gaatttccgg
     8221 attccgatac aaaaatatgt aatgaattga tcaatgcatt atctcaggaa ctcggtgaaa
     8281 aaaacgtccg agtggaagag ggttgaattg catatcctat catagctttt tcgatccgat
     8341 gataaaattt agtctatatt ttgataaagc ggtaagagaa aggataaacg aatggaatta
     8401 atggaaccag aaaccccgat ccgatctata gcggttttga cttccggagg agatgcgccg
     8461 ggaatgaatg cagccatccg tgcggttgtc cgtgcgggaa tctataaagg gctgaaagtt
     8521 tatggcgttc aggacggatt taaggggttg attaacggtg actttgtacc gcttggaaga
     8581 cgagacgtca gtggcatatt aagtcatgga ggtacgttcc tgcgcacgga tcgcagcagc
     8641 gaaatgaaaa ctccatatgg tcaacgtgaa gcagttcggc aattaaataa tgagggaatc
     8701 gacggcttga ttgtgatcgg cggtgacggg tctatggccg gagcaaaggc actctgtgat
     8761 gaaggaaccc gtgtcatcgg tattccggga acgatcgaca atgatgtttg gggaacggac
     8821 agttgcgtcg gtgtcgatac ggcactgaat acgattatgg aggccgtgga taaaatccgc
     8881 gatacggcgg catcccacac ccgcgcattt ttagttcaaa cgatgggtcg cgagtgtggc
     8941 tatcttgccg tccaatccgc tatgatctcc ggtgccgaaa tggcgcttat tccggaggat
     9001 cccgtttcgg tggaagagat cgctcagacg gttgaaaacg cttataaacg aggcaaaaga
     9061 cactgcatca tcattatggc ggaagggtat ccgatcccga tggtggattt ggcaaagcag
     9121 ctggacgaaa tggatttggg ttttaccacc cgttcgatca atttgagtta cgtccaacgc
     9181 ggcggatctc ccacttcgtt tgatcgaata ttggcttctc gaatgggata tgctgcggtt
     9241 gaattaatca gttccgggca gacttcctta atgactgcga tgcaagggcg ggaaattatc
     9301 ggacttcctt taatcgatat cgtcgggaaa catcggccca tttcggaaga atatttacat
     9361 atttcgaata tattatcccg ataattgcat caaattaaaa aacgattgat tgttacggat
     9421 caaatttaaa aaactgatcg gattgtccga tcggtttttt tgccagttaa aaaaagaaag
     9481 taaatgatat ttgtctataa aactcattac ctttgaagag gttattttta tggatgactt
     9541 ttataattaa gtttaatggt acgaacaggt aaatcctgaa aatcggtaaa tcaataaagg
     9601 agaatcgtat gaaaaaagtg tatcttttgg tcgtattcgt tctgcttgcg cttttagttg
     9661 tcccggttgc cgctcaagag gctgaaccag gcgcaaagct ggtcatcgaa agctggcgtc
     9721 aggatgacat tccgatttgg aatgatgtca ttattccggc tttcaacaaa atccatccgg
     9781 aaatccaagt aatatttcag cccacaaagc aggaagatta caatggtcaa ctgaataccc
     9841 gacttgccgg cggaaccgcg ggtgatttga ttacctgtcg tccatttgat ccttctctga
     9901 aattatacga gtccggatat ctcgcaaatc tgaacgattt gccgggaatg caattttttg
     9961 acagttctgc caaagttgcc tggacgaccg atgacggatc cgtcacttat tgtgtcccga
    10021 tggcatcggt aatccacggg tttatgtata acaagaccgc ttttgaacaa aaaggctgga
    10081 aagtgcccgt aaccaaagat gaattttggg cattattgga tgcgattaaa gccgacggtg
    10141 aatatattcc tttggcgatg ggtactcatg acttatggga agcggcgacc atgggttggt
    10201 ataacatcgg cgttcagaac tacaaaggcg aggaaggtcg tttggctatc attaacggaa
    10261 ccgcaaaatt cacggatcct ccgtatgtaa agacttatga agaattagcc aaatgggcac
    10321 cgtatttacc ggaaggatat caatccgtcc aatattccga tgcgcaacag ctcttcacga
    10381 tggggcgagc tgccatttat ccgaccggtt catgggaaat ttcagtcttt gagccgatga
    10441 tcaacggtgc gtttgaaatg ggagctttcc gagttccgaa ggataacgcc gatgatcctt
    10501 gctatatcag tgatcatacc gatatcggta tcggcatgaa tgccgccacg aaatatccgg
    10561 aagccgtaaa aatcttcctg aactgggttg ccactccgga atttgccaca ctctatgcaa
    10621 ataacatggt cggattctat ccgttggcaa agattgacta tacaatcgat aatccggtgg
    10681 ctcaggaatt cctgtcttgg cggaaagatt gccaatccag cattcgtatt tccgcttcca
    10741 ttatcggacg cggcgaaccg gggatggata ccgatatttg gcagacttct gccaatgtta
    10801 taaacggtac gatgactccg atggaagctg cgcagtatgt ccaggacgct ttggcgaaat
    10861 ggtataaacc gcagcagaag taaacgataa tctgtaacaa ttaaaattca attacaaaaa
    10921 gggagagtct taaagtcatt tgactctccc tctttgcaat catttccgtt tttttaaagc
    10981 ttgaacgtaa aggataaatt gtttatattt tgatccgata tctattttta taaaaaaagt
    11041 aatttgatga atttgtccca atttggaaac ttttcaggat gttgaaaaag aattgagaat
    11101 tttttatcaa ataattttta aatcactgct gaaaggaaga atgcctgtat gaatgctacc
    11161 cgtaaagtcc gcaaaccgtt tccatggcat ttggttgttt ttatcgctcc ggccttgatc
    11221 atttatgtta ttgtgatggt tattcctctt ttggattccc tgacattaag tttctttacg
    11281 gaagatccca ataatgctcg aaatacaatt tttgtcggaa ttcaaaacta taaatatctg
    11341 tttaccgata agtattatgc tccgatcttc tggggagcct tcaaaaataa cattgtcttc
    11401 ttccttattc atatgttggt acaaaatccc atcggtctgc ttttggcatc ggtcctttgt
    11461 aaaggcggta aaattcggaa tttctttcgg accgttctgt ttattccgac gattttatca
    11521 tttgtattgg tgggctttgt ttggaaactg atcctgagcc cggtttgggg cgtatccaaa
    11581 acgattctca atatattcgg actcggcggg cttttccgac cctggctggg cttggaagaa
    11641 acggcgttga taacctgttc attaatctcc gtctggcaat atatcggcac gcctatgatt
    11701 ttgttctata ccgcattgat tgccatcccg gatgaattga cggaagcggc gtatgtggac
    11761 ggtgccaacg cttggcagat tttttggcgg gtgaaatttc cgttgatctt acctacggtg
    11821 ggcttggtgg caatgatgac ctttgtcgga aactttaatg cattcgatct gatctatacc
    11881 atgcaaggag cgttggcggg gccgaatctt tcgacggaca ttatgggaac tctgttttat
    11941 cgtacctttt tcgggcagca acagcaaatg cccaatcaaa cgctgggcgc gacgattgca
    12001 gcgatgcagt tcattattat cctgatcggt gttgttattt atatgatctg gcaaaaacgc
    12061 accaccactt atgaatattg aggaggaaaa aatgaccgca accgagttaa acaagaaaaa
    12121 aggcgcctct gtctcttatc ggatcatttc atggctgcga agtgccgggc ctattctatt
    12181 tttgattttt tggagcatta tcgcattatt cccaatttat attattttgg tgaacgcgtt
    12241 taaaaatcgg aatgcgatat ttaaatatcc gtttaatatc cccaatgccg aaactttcag
    12301 cttgatcggt tttgaaacag taaataaacg agcgaatttt gcccgatatt ttaccaacag
    12361 tattgtggtc acggtagtat cgttggcgat tactttatta attgccacca tggcatcttt
    12421 cgctttatcg cgctataaat tcaaaggagc tactttatta agtctgatct tttccatcgg
    12481 cattatgata cccatccggc ttgcaaccgt aggaattctg cggctgatgg tgaattggtc
    12541 cttaacaaat acgttgcttt ctttgattat cgtttacatc gcacaatctt taccgatggc
    12601 gatctttatt ctttcttcgt ttatgaaaca gattccgcag gaattaattg atgcggcaag
    12661 ggtggacgga gccagcgaat accggattta ttggatgata ttgcctctga tacgaccggc
    12721 gctgggggca atcgggatat tttcgatgat accgatttgg aatgatttgt ggtggccgct
    12781 gattttggca ccgagtgcgg aaacggcgac ggtaaccttg ggcgcgcaac agttcctcgg
    12841 ccaatatatg acggattgga atgcggtatt ggcatcgttg accatgtcaa tcattcccat
    12901 tctgatcttt tacctgatat tctccaaaca aatgatcaaa tcgatcacat cgggtgcgat
    12961 caaatcataa tccaatgaaa agacggaaag agagggaaca cgaatgaaag taggaatagt
    13021 cggtaccggt tcgatgggtt ccacccatgc catggggtgg gcggaaacgg atgcagaaat
    13081 tgtcggtttc tgcgcggaat ccgttccgga agccgaaccg ctcgccaaga aataccatac
    13141 aaaagttttt gagaagctgg atcaattgtt gtccgaagcg gatattgttg atatttgcac
    13201 tcccacttat ctgcatccga ctcaggtgag acaagcggcc aaagcccgaa agcagattat
    13261 ttgtgagaaa ccgttggcgt tggactggca atccggattg gaaatgctga gggcttgcga
    13321 ggaagccgga gtcagactgt tggttgcgca ggttgtccga ttcttccccg aatatgcggc
    13381 ggctaaagag ttggtcgaca gcggccaaat cgggaaaccc ggaacgattc atttgcgccg
    13441 ttgcagttat cgtccgaaaa agccctcgaa taattggttc ctggacgaac agaaatcggg
    13501 cggcattctg atggacctgg caatccatga ttttgattat gcaagatggg tggcaggaga
    13561 tgttcggacg gtctatactc agaaagtcag ttctttggat ccgtcctcac cgattgattt
    13621 cggattgacc attcttaccc atgaaaacgg gacgctgact catgtcacct gtgggtgggc
    13681 atatccccct ccgaatttcc agacgggttt tgatatcaat tgcgaacggg gagccgtcca
    13741 ttttgattcc gaagaaacca tgccgattca taatctgatt caaaccgata aagatgctcc
    13801 ggatgtcgca ttaccgggga gcccgctggc ggaaagtcct tatacgagcg aaattaagga
    13861 gttttatcgg gcgatttgtt acggagatga ggtacgggta acgggaatgg acggcgtcaa
    13921 agccgttcag atcgcttgcg cggcgcggga atccgcccgg accggagaac cggtgcaaat
    13981 caaaccgctg gaaggagttt tgaaatgatt aagatcggga tcatgagttt tgcacatttg
    14041 catgcggaag cctatattca gaatattcgt tcaatcccgg gggtggaatg gatcggtttc
    14101 gtcgacccgg acagcgagcg agtgaaacaa ttctccgaac aatttcggac atggggaacg
    14161 agcgattttg atgagttctt tgccaagaaa ccggacggtg tggtgatctg cagtgaaaac
    14221 aacaaccact tgtcgatggt ggaaacggcg atcgccgccg gcgttaaaaa tatctgctgc
    14281 gaaaaaccgc ttgccaccac cttggaagat gccgaaaaga ttgtttcatt aacggaatca
    14341 aaaaaaatca atctgatgac cgcatttccg atgcgctttt ccgctccgat cgtcgcggta
    14401 aaagatcagt tggatcgggg ggtatacgga aaaatttact gttttcgttc ggaaaatgag
    14461 ggtcagatgc cttccctcca ccgaaaatgg tttgtggata aaaaattggc gggcggcgga
    14521 gccttacagg atcatttggt acatttgacc gatctgtttc gttggatcac gaagtcggaa
    14581 gtggacagcg tttatgcaca atcgagccaa tgttttcacc gcggtgaagt ggatgtggaa
    14641 acggccggga tggcgatgct gcatttcaaa aatgactgct ttgccacaat tgattgctcc
    14701 tggtcccgcc cggattatta tccgacttgg ggaggcttga attttgaagt ggttaccgat
    14761 cgcggagcgg ttcaggtgga tgcgtttcgc caaaacttat ccgtttaccg gcatgatata
    14821 caaaatcagg tttggcatta ttggggttct gattcgaacc aggggatgat cgaagaattt
    14881 gcggcttcca ttcgcgagga acgccccgca tgtatcacgg cgacggatgg gcttcgcgcg
    14941 gtggaagtgg ttcaagccgc ttatgaatcc gcagcttcgg ggaagacggt gaagctgtaa
    15001 ggtctttttc caaaccaacc atttatgaca gcttatggtt tcgcgtagtt atttgaatca
    15061 aataaatatc aaaaacattc cgctttcagc cgatgtagtc cgaaagcgga atgttttata
    15121 tttaaactga cgttacaatt aattgataaa gaaagtgagg aaatgtcatg gatgtaaaaa
    15181 ttacctggtt ggggcattca gcctttctga tcgatttcga tcattataaa gttctgttgg
    15241 atcctttttt aaccggaaat tcgaatgccg ccataccggc aaaaaaagcg gaagcggatc
    15301 ttattttagt cagtcacggg cacagtgacc atgtcggaga cgcgattgaa atttccaatc
    15361 gctgtcatgc cccgattttg tgcaattttg aattatcgaa ttggttcgcg aaacacggcg
    15421 cggtcaatac gatgcctatc tatgtcagcg gaacgcgtgc attccccttc ggaacggtgc
    15481 gggcgacgat cgctgtgcac ggttcgggaa ctccggacgg aacgtatggc gggttggcat
    15541 tggggtttat tttggcaacc cacgatcagc aaaaaaagat ttacttcgcc ggagatacgg
    15601 gtgttttcag cgaaatgtct ttgatcggtg aagatgcggt ggatgtcgct ttactgccga
    15661 tcggagatat tttcgtgatg ggcttatctg aatcgattcg ggcattgaaa atgatccagc
    15721 ctaaaaatgt gattcccatg cattacgatc ccggattgaa tctcgaaggt tggaagcggg
    15781 aagttattga aacgtccgga gccaaaccga ttgttctgag aagcggagaa agtttcgatt
    15841 tatagccgaa aaatccgatt gtgaaacgat tatttcagat cgatttcgcc ggcaagcatc
    15901 ttcgcgcgct gccaagggga agcgtcggaa acatcggcaa ccagtccgcg cagttcataa
    15961 acctgatcgc gcaattccgc cgcccgttcg aagtccagat tcttggctgc ctctttcatt
    16021 tctctttcca ggtctgccaa gatgtgtttc agttcgttct tcggcaaatt cttacgagtg
    16081 gttttataat ccgccttctc ttccgctatc gcgttggaaa cggttaatcg ctctgaaagg
    16141 tcatatattc ctttgacaat tgtcatgggt gtaatatgat gtgcctcatt atatgcctgt
    16201 tgttttttgc ggcggcgatt ggtctcgtcg atggcatatt tcattgaatc cgtcatttta
    16261 tccgcgtaaa gaatgacttt accgtccacg ttacgagctg ccctgccgat ggtttgaatc
    16321 agtgagacgc cggatcgtaa aaagccttct ttatccgcat ccagaatcgc caccagtgaa
    16381 acttccggca aatccaaccc ttcgcgcagc aagttaattc cgacaatcac atcatagacg
    16441 ccggtacgca tatctcgcag aatggaaatc cgttccaagg tatccacttc ggaatgtaaa
    16501 taatgcactt ttatgtcatt gtccttcaga tattcggata aatcttccgc catgcgcttg
    16561 gttaatgtcg tcaccaaaac gcgttgattg gcagcggttc gcttacgaat ttcgccgatc
    16621 aggtcgtcaa tttgaccctt tgtcggtcga acttcaacct gcggatcgac caacccggtc
    16681 ggacgaatga tttgctcggc gatttgttgc gaatgttcca attcataagg ccccggtgtg
    16741 gcactggtgt aaatggtgta tcccattgtt ttttcaaatt cttcgaaagt taacgggcga
    16801 ttatccaaag cggaaggcag gcggaaacca tattcggcaa gaacggattt gcgactgcga
    16861 tcaccgtgat acatcccccg aatctgaggg atcgtcatat gggactcatc gataaacaac
    16921 agatattccg aaggcaggta atccattaat gtccaggggt gacttcccgc gggccgctga
    16981 tccaaatggc gggaatagtt ttcaattccc gaacaataac cgatttcctt gatcatttcg
    17041 atatcaaatt ttgtacgctg ctcgatccgt tgcgcctcaa gcaagcggtt attgtctttg
    17101 aaatatttga ttcgctcttc cagttccgtt tcgatatcga caatggcttg ctttatgcga
    17161 gattcttcgg tgatatagtg cttcgccgga tagattgcta tttcatccag tgtggtgacc
    17221 aactcgcccg tcagcggatt cacctcagta atcttttcaa tctcatcgcc ccaaaaggaa
    17281 attcggtagg ccacactatc ctcatatgcc ggaaatattt ccaacgtatc ccctctgacc
    17341 cgaaacgttc cggaatgaaa atccatatca tttcgttggt attggctttc gaccagttgg
    17401 cgcaacaacg cgtttcggcg ggtgatggta ccgactttta aattaatcac cgttttcccg
    17461 taagcttccg gatctcccaa cccgtaaatg caggaaaccg aagcgacaat gatcacatcc
    17521 gatcgggaag ccagcgcggc ggtcgccgcc agccgcaacc ggtcaatttc gtcgttggta
    17581 tcgacttctt tttcaatata caagtcatgg cgcgggacat aggcttccgg ctgataataa
    17641 tcataataag aaacgaaata ttccacggca ttcttgggga agaattcctt gaattcggcg
    17701 taaagttggg cggcaagggt tttgttgtgg gccatcacta ccgcgggcat ttgtaattgt
    17761 tgaatgacat ttgccatggt aaatgttttg ccggtgccgg ttgcgcccag caatacctga
    17821 tgtcgatacc ctttttgaac cccatctacc aatgctttga tcgcctcggg ttgatcgccc
    17881 atcggctggt aaggggcggt taattcaaat ttatttctca ttctttctat actccagaat
    17941 tattataccg tcgcggttta cagatattta ttaatattgt ggaagagaag gattgtaagc
    18001 cgtaaaaaat ttataaaaaa aaagaagaat tttccggtga ccggagaaga aataaggttt
    18061 tatttctcag ggtgtcggaa tctcaaattc aaagctgata ctcggccaaa gacgcacttt
    18121 tttcagtcgg tatgagtgag gtgagaatca atcagatcaa gactcggatc aatcatcgcc
    18181 ggtttctatc tttttcacca ttgtccatgc cggttccgct tcctcgcgga cggcgccttt
    18241 gtggtcgatg aaactccatt tcccgggatc ttcgcggaga acggtaaaac cgttgcgttc
    18301 gtaaaatgct ttgcaccgtt tgttggtttt tgcgcaattc agaaagacat tatgaatatt
    18361 ccgatcgcga gcggtaactt cacaaatttt taacagtaag gtaccgagtc cccggttgcg
    18421 gaaatcgggt ttgacgcgaa aagaactcag aaacatatac ggttgttccg ggtcgaatgc
    18481 cggatcaggc tgcttttccg tcaaaaacac ctgaccgatc acgccatatt tctcgatctc
    18541 ggcgatccat ggaaaagccc ggccggcaag gaaatttcgg taaacttcac ggtaaatttt
    18601 ccgatatcgg acatattcat ccccccattc gatcagagga agatccgatt ctctcatcaa
    18661 gcgaatattg acactgtcaa gccaagtttc cataatgatt ttccgtgtct gatgatattg
    18721 taaacggttt atatctcggc ggctggattt cccgatgaat gtttcttgtc caagcttttg
    18781 gtaaagagcg gcaaaataaa ttccaatgtt ttttcctctt cttcacggga actgatttcc
    18841 tcgtcgatcc atcctttgcg taagcggtcc aaaaccgccc cgatccaact tccggccgga
    18901 tagggcaacc ggcgcagctc cgccccggtg gtaaacggct tcatatttct ccaagttttc
    18961 aggaagtttc gaattgtttc atggactttt tcgtcgtcgc ttaaaatatc atagcaataa
    19021 acggcttcaa gcgattgctt ttcgagaaaa aaggtcactt cgctcggcca ggaatgaacc
    19081 agatcgggca ttttctgaca gatttcggaa taagaaagca acattttctg gatgcgttga
    19141 gaaagttgca ggcgttgaac gatctcttta atggtttccg gagaattttt cccccaccag
    19201 attaagtatt tcccttccag attgataaat tccggtaagg aaggaagggc ttccaaccaa
    19261 taatccggcg gatctttttg actgaatttt gtcaggttgg ctgtattttg agaagtccac
    19321 cggatattcg gatgaatttg ttccaacaag ccgagttctt ccaagcgtga aagtccggcc
    19381 gaaggggatt cttcatgaaa gaaaaggtcc aattcattca taattcggcg ccccgaaatt
    19441 cgtttgagca acgtttttcc gtctttcagc aacatcatcg tatgtttttc gatggtaaag
    19501 ttgaatcttt tctcaaaacg caccgcccgc aacatccgcg tcggatcatc cataaaagag
    19561 agggaatgca agacacggat caatttctga tgtaaatcca ggtaaccgcc ccaataatcc
    19621 aataagtcgc cgaaataatc cccgtccaaa cgcaaagcca tggtattgat agtgaaatcg
    19681 cgacgatgta aatccaattt gatactgctc cgttccacct tcggcatggc agtcgggtaa
    19741 tcataaaatt cggttcgggc ggagatcaaa tccaaagtag ccggcaaatc cgattcggga
    19801 gcgccctcgg cggaaggagg ggtattcttt attatctgat catgaatatc ttcgatgacc
    19861 cattttgccg ttccgaatcg ggaatgaata cggacttttc ccccgtaaat atgcaccagc
    19921 gtccgcgcca aatgaatggc atccccttcg acgacaatat cgaaatccat tacgggttgt
    19981 ccgaggatca ggtcgcgaac aaaaccgccc acaatataaa cgggatagcg acaattccaa
    20041 gcggtatcag ccaccatatg gatcaaggtg agatatgagg gaggcaaaac acgagttaaa
    20101 atctgtgcat aattgcgatt gccggggaca ctgctgcgac cggtaaggat cttaatcaga
    20161 tcggtacggg tcacgattcc gataatctca tgaccgtcac cattaatgac gggaatctga
    20221 ccccaaccgg aaagattcat catttctttc agataatcta ccgaatcttc cggatgaaca
    20281 ctgtaattgc cggggtccat aatcgattcg accgacgggg acaatccgaa agccagcgcg
    20341 cgatcgacgg aacggcgatt caacaatccg atcagattct tattcttatc gacaaccgga
    20401 tagccttcat agccgtaacg ctggatgagt tcggcaattt cttccatcgg catttgggaa
    20461 tcgattaaca gagggcgggt tgacataat
//
LOCUS       DAOSMM010000041        19532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2788_length_19532_cov_19.503774, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000041 DAOSMM010000000
VERSION     DAOSMM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 19532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19532
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_19532_cov_19.503774"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1818
                     /locus_tag="PK898_06975"
     CDS             82..1818
                     /locus_tag="PK898_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19812.1"
                     /translation="MNSFLKYAKPYKKYFLFGPIFMVIEATGDIVLPFLVAQIINNGA
                     VNHDIPYILRTGGLMVGFSVIMAVFGTLGAKFAVNGSVNLAADLRFDVFSRIQEFSFK
                     NIDDFSTGSLITRITNDITQIQTFAMSLLRGVFRSPVMLIGAVIMAFRLNSQLAIVIL
                     IVIPVLGSAIFTIITKASPRFTTMQEQLDKLNSDISESLTNIRVIKSFVREDYENKDF
                     SVVNEGLREKSIAAMKMVILQQPVITATMNITTLAVVWFAGRQIIVGSMPIGNLAAFI
                     TYITQILMSLNLLANIFLQGSRAIASSKRINQVMETTIELDDSHAAFPEAMVEKGEIE
                     FRHVSFRYFKTNHENVLDDINLAISAGETVGMIGSTGSGKTTMVQLIPRLYDADEGEV
                     LVDGINVKDYSLKKLREGVALVLQKNTLFSGTIAENLQWGNEYATEEEMRAAARIAQA
                     ESFIDSLPDGFETDLGQGGVNLSGGQKQRLCIARALLKKPKILILDDSTSAVDTATEA
                     KIRKAFSENLQGITKIIIAQRINSVIDADKIVVLNEGKIVGCGVHQDLIKTCVPYQEI
                     YYSQKEKNKEGE"
     gene            1818..3701
                     /locus_tag="PK898_06980"
     CDS             1818..3701
                     /locus_tag="PK898_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19813.1"
                     /translation="MNTQRMERLAKKYGNTNHNGVAQPKTFTINTMSALRAKSLSSND
                     KPKNMGYTIKRLLDYLENDKKLLITAIACILFQTIATLAGSYMIRPIINTYITPKNGQ
                     GDLHGLANSLVILAFIYLSGVILQYLQLRIMVGVSQRSLVRLRRDLYNKLLNLPIRFF
                     DTNSSGDIMSRFTNDVDAVGEMLNTTIVQLVAGTITLIGTLGLMIYTNIWLTLITLVM
                     IPIFTYASRSIIQFSRKPYSQQQTALGILDGFAQETINGQKVVKVFTREEIVKDEFKY
                     LNDDLRRKQIVAQFRSSIMGPVTHNLSNFSYALTTGIGSILCITQGFDIGGLTVFVNY
                     TRQFSRPINEISMQMNTVFAALAGAERVFELLDEPPEPPDAPDCVNVDHLAGHVVMSH
                     VNFGYYPDKLVLHDISLYAKPGQKIAFVGSTGAGKTTITNLLTRFYDIQSGEITIDGI
                     PIDKIQRKILRRNIAMVLQDTHLFTGTVRENIRYGRLDATDDDVITAAKVANAHSFIM
                     HLEKGYDTMLENDGANLSQGQRQLLNIARASISKAPILILDEATSSVDTRTERHIEKG
                     MDALMSDRTTFVIAHRLSTVRNANAIMVLEKGEIIERGDHDDLLQQRGRYYELYSELA
                     ELD"
     gene            3914..5131
                     /locus_tag="PK898_06985"
     CDS             3914..5131
                     /locus_tag="PK898_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU19814.1"
                     /translation="MESNASQNGQGATSEPFDNMETLLESKGLNIDFPQQGEIRQGVI
                     ASITPDQILVNVGTKSEGIISGKELEAISKSERDSLNVDQKIAVYILDPEDSNGNLVL
                     SYTRAREEESWKRAEQLLNSKEPFETKIVGYNKGGLIVELGSLRGFIPASQISLSRRV
                     DVSGETPEQRWGKMVNQPIKVSVIEVDRERRRLICSERAASTETRESLKEHVIESLKE
                     GDIRTGRVASLADFGAFINISGADGLVHLSEISWDHIKHPSEVLQVGQEVKVKVISID
                     KEKKRIGLSIRQTQEDPWAERVAKYQVGQLVEGTITRLTKFGAFAKLDDNIEGLIHIS
                     EISEKRIDHAKEVLHEGEKITLRVIKIDPANHRIGLSVRRVDSMAYADMDWESLLDSD
                     LEAVEKSTEDEEK"
     gene            5343..5897
                     /locus_tag="PK898_06990"
     CDS             5343..5897
                     /locus_tag="PK898_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOU19815.1"
                     /translation="MKNQTFYVLALFDEKTNQIFTELTENLKKNNIDFLEELPHITLG
                     GYTEVPESEITSWVKEFSETHCPLKINFNHIGLFPQSICFIGSKVNEELLRFHREFHA
                     NFDSCFTEIGYNFTLCSENWVPHMTIITGNAENIFKGIPILFSAFQPFIGTITELAIY
                     QFDPFLELRRFPLKHTDFQTDKKR"
     gene            5926..7017
                     /locus_tag="PK898_06995"
     CDS             5926..7017
                     /locus_tag="PK898_06995"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOU19816.1"
                     /translation="MNFIIDGHEDLAFEALANQRDTTRSVAEIREKEPQNTENQVTLG
                     WPEMQAGKVGIIFATIFIEPPAHQTPPKRTGVNYGTPDEFHQSVRKQLDFYEQWEDSC
                     PDKFTIIRSLSDLDQTISRWQREVENPTGSTLPVGLVLLLEGAEGLRKEDDLDEYYDR
                     GLRIVGPVWEGGRWCGGTYSPDSMRFTPEGKRFLEILASKGFILDVAHMNTFSADEAL
                     DLYEGTVIASHCNCRRLLKSPENQRHFDDETIRKLIDRDGVMGVIPFNNFLDTGWKNG
                     MPRNRVTLDLLAQHIDHICQLAGNADHAAIGSDLDGGFGYPNIPEEMNDIQDLQKLEQ
                     ILQQKGYTPEDIMKIFNGNWISVLKRGLP"
     gene            7014..7529
                     /locus_tag="PK898_07000"
     CDS             7014..7529
                     /locus_tag="PK898_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19817.1"
                     /translation="MTEQNNEIPGNETQSEEIRAQKRLRNALFVTLGGYLILVLGAKP
                     SIFGLDRSPVIGFVQTATFIVGLGIICLGGRMSLRSFWKGKKISLTADFGLRVVATGY
                     VIAIFTAMADVFGFGSHPLPGVPFFGPLQARGVILGEAIIAVGLIMMFSPVDETEEKS
                     KDRQSNSEKSA"
     gene            complement(7533..8285)
                     /locus_tag="PK898_07005"
     CDS             complement(7533..8285)
                     /locus_tag="PK898_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19818.1"
                     /translation="MTGCDQVNIYSLTEPTETMTFQNQKTLVPTAIDSPTVTVQWFPA
                     TATPRPLSTPTAFPTKDMKPDVGEVLLEDVFLNENFWQTFRSDQGNAVISNNELTLAI
                     QFSAGSIASFLDYHPKTDYYLSVDVTLPICSDSSDYYGVFFRYGDKDNYDRFLINCQG
                     QTRVEQRYGGKMLPLADWSVNGQIRSGGPQKFTLGMRIKGETLRFFINDVFLYETKDS
                     LLKSGAIGLLANSQGVSPISVSFSNFKIYDLR"
     gene            complement(8346..10766)
                     /locus_tag="PK898_07010"
     CDS             complement(8346..10766)
                     /locus_tag="PK898_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOU19819.1"
                     /translation="MDKKSLKMLEYPEVIRRLAQYASFNVSEQMAQNLHPETDSSIIR
                     YKLSLTTEARKLLSVNDTFRLGGCSDLRPYLEVARKQGTLEAKAFVEIAYTLSVARDL
                     YRSLSHHEAEYPNLAAIAENLIPPAGLIERINFTVNDRGEVLDSASEKLTSIRREIRL
                     SYNRLLGRLEKMVKESRYQPMLQEPIITQRNGRYVIPLKAEYKGQLKSIIHDQSSSGA
                     TLFVEPLATVELNNVYQALQLDERNEIIRILQNLTAFVGDYADQIEQSVEALAEIDFA
                     MMCAKYADDLRAAEPLLVDPYKKTGNPVIKLYQARHPLLPVETAVPIDVVPDEETFAV
                     VITGPNTGGKTVSLKTIGLLILMAQSGLHIPVRSGSEFSVFKNVFADIGDEQSIEQSL
                     STFSGHITNIIRILKEADSKTLVLLDELGSGTDPQEGSALARAILQYLIKKHIPCFVA
                     THFSELKTFAHVTPGAINASMEFDLKTLRPTYRLSIGLPGRSNALLIAEKLGLPAEIL
                     EDARETIDPEVQDTGALLDEIHRQQEAARKARSQADRLRSLAENDERKLRKQLEEIEA
                     EKEKILSEAHSETEEEIKELKTELAKLRKAYAAAKIPLGELKPLEEGLKESERAAQSI
                     ENHIEKKSKLKTYHAAPLKIGEKVRVRSLGTNSLATITTIDGDEAEILLGALRMRVPI
                     KDLIRKSDDTGNNDENTGGPTGKTESPVKMEERKGSGEIFHPSPGVEIDLRGKTAEEM
                     TDLLEKYLDEAVLAGLPYVRIIHGKGTGKLRQAARQFLLDSPYVKYIENGKDNEGGEG
                     VTVAHLKV"
     gene            complement(10786..12108)
                     /gene="miaB"
                     /locus_tag="PK898_07015"
     CDS             complement(10786..12108)
                     /gene="miaB"
                     /locus_tag="PK898_07015"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU19820.1"
                     /translation="MMKYYIWTEGCQMNVADSQRLASALERLGYSESPIIEEADVIVL
                     NTCMVRQSAEDKAIGRLSSLKPLKKKNPDLVLGVMGCMVGFKDQTEIRKKLPYVDVFA
                     PPSNPKPIIDFLIRQEYIGEASASRNVETDTVWEPGKLTLPANRRKKTVAGFIPIVYG
                     CSHACAYCIIPYKRGAERSRNPQEILDHARSLAEQGVKEVTLLGQIVDRYGLDTAGYP
                     TLAELLREIHEIPGIERIRFLTSHPNWITDELLDMVRELPKVMKHIEVPAQAGNNTVL
                     ENMRRGYTDEEYRNLISHIREKIPGVSIGTDIIVGFPGETEAQFEDTFRQLADLKLNV
                     VHLARYSPRKGTLSERTMPDDVSDEEKWRRFRAVETLQERIATDLHAALEGKILPVLF
                     EEKQKGRWMGRTEHMDLVYVESESDLTGLILPVKINWTGPWTMIGELV"
     gene            complement(12237..13001)
                     /locus_tag="PK898_07020"
     CDS             complement(12237..13001)
                     /locus_tag="PK898_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU19821.1"
                     /translation="MGNDDHTQKISAQAAERGEPSYVWRDGQKRRLKMIQTTAEGRDS
                     GVVLENGCGVGAYLKRLAPVATFSVGLEVEFDRAREALNLVGGERSSVINAVGEELPF
                     PEDTFDLILSHEVLEHVDDDVACLREIVRCLKPGGRLILFCPNRWYPFETHGIYWKGK
                     YHFGNKFGVNYLPRKLRDKLAPHVNVYTTKDLQRKLNGLPVEVISRTVIFGAYDNIIA
                     RSKGLGLMLRKILLFLERTPLKALGLSHFWVIEKKG"
     gene            complement(13006..14697)
                     /locus_tag="PK898_07025"
     CDS             complement(13006..14697)
                     /locus_tag="PK898_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOU19822.1"
                     /translation="MKTAGVGEIIPKIDSFYQDCLNLLNEISIESLLNRTAEIAISVT
                     NATYCICGTTDEEDTFTKIVLRGFTQVELDLIHSDEEAAGQPIRNIHSDAIINIARGK
                     DLPENYNIFPAKRPFISSLLYVPIFYGKEKMGSIILLNKIGAPQFDSIDQKFIEILAR
                     YAGFGINNTIIYERIANREQMLAKRNEDLALLNELAKISASSTENQDAIVHETMHQVM
                     GYLDLVVGEVFLRDDDAPNQFKIVFKEGHNLPTSLFGFSTVQMGQGIVGKVAQSKRNY
                     LLSEEELEIINQKRATTVDLNYVAVLPLITSEGVVGVSCLGTKLVDEKDTLNLQFLSS
                     ISSWMATLIQDYRLTLQRKRVAILEERERISMDLHDGVIQSIYGVGLTLEHARILAKA
                     DPEKVEEQIQASIDALNATIRDIRSYIMDLKPARLTGENLLQSLRRLGNDFYSNTLVT
                     TVFDPKVETLDQISEDYANAFYMICKEALANITKHAKATQVMITFTEEPDRYVLIVQD
                     NGSGFDPAKGRKPESHGINNMFARAENLGGKLEMTSKPGKGTKLEVWLPKTAKEG"
     gene            14914..15753
                     /locus_tag="PK898_07030"
     CDS             14914..15753
                     /locus_tag="PK898_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOU19823.1"
                     /translation="MAKKQEIENNSFTIRDITDVTPAQRIAVLIDGDNAQASLIDKIL
                     AEITKYGLITIRRIYGDWTASNMSGWKDVLQTHAIQPIQQFRYTTGKNATDSAMIIDA
                     MDIFYSGTVDAFCLVSSDSDYTRLATRIREQGLFVMGIGKKTTPRAFVNACDLFVYTE
                     NLVPQNEPLKSTGTTANSAHKKKTAKSAESSPAPDVENGNSPDPLPLLRSAFDLATRD
                     NGWAFLGTLGHHLQQLDPGFDSRTFGYRQLSLLIQAYPDVIETKEVKGADGTSTIYAR
                     LKS"
     gene            15773..16381
                     /locus_tag="PK898_07035"
     CDS             15773..16381
                     /locus_tag="PK898_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19824.1"
                     /translation="MASSSFLSSVRATIEPCKKGFKRKFPIWMWQSGLGNVLNAFPSL
                     TGEQVVVILPDNTKLALNCTEKDDVIYALLPAMENPEIYRTLVANPAVQIWSKNGWYN
                     AGARLLTFEETAKTLSSFQPESFFGKIGASLRNPDQDARIRMIEIRRISACTGEKGPG
                     QYAWVWAASSLFFFFSWARQRKINKRTAHKLKVCSSQSNKKK"
     gene            16396..17640
                     /locus_tag="PK898_07040"
     CDS             16396..17640
                     /locus_tag="PK898_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU19825.1"
                     /translation="MNSYNNNTNFVSSSPDSNRKQPRRSFPWFTAFIMILIGCLIGVL
                     ISLNFVPEPKPETSASAEQIENLQATVTALQNTLKQSQPAANTLNVSYTNVETEITNV
                     VEKVGPAVVTVTANIPTTASYYWFQTSYVATSMGSGVIISDQGYILTNNHVVEGATEL
                     SVELSDGTTLPAKLVNTDEFTDLAVLKVDGTMPGVAKLGESNLLKSGETVIAIGSPLG
                     NFKNTVTVGVVSATGRFLENDKGYKMENLIQTDAAINQGNSGGPLVNLAGEVIGINVM
                     IVRGSSTSSTYAEGLGFAIPSDTVRLIAQQIIEKGKLTRPYLGIQWVDITPRLAENYG
                     LPVKYGVIVLAIEPGSPADQAGLKRNDIITKIGDYEITENSAYYNCLFKYSPGDKVTL
                     EVIRNGKKINIDVKLGGDTTAL"
     gene            17654..19393
                     /locus_tag="PK898_07045"
     CDS             17654..19393
                     /locus_tag="PK898_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19826.1"
                     /translation="MKDAAGQLSRKSIQKVMIISAIEGLVALFFTFLHPSENESAVFA
                     GYSAQRFILGGITLVGVALFLLIGKLFSENHPRSEQRVQSLNDFLRQNDRIFFSLVIL
                     SGLTLFAIWCFVFSWLFIPANLRPQIIWVGLIALQILLCIRRSYGETIRQNHYAEKYR
                     LFPRWKDLSANQKKVFRFLVILSVAYILILIPSNLNGSQNMEAFARYGGDEFVIYPIL
                     MDIMKPGETFSATLYHLFIYEDYHYGYPFYAWSTLVLLPIKWISGENFAEQIQINLPL
                     LRIFVSVIPVILACLVLTFLFTRWKSFWISAPVFLFLLSAPGTLQNNQGFWHPDGLNL
                     LFVCLTLYFLQRDRFRYGRNFYLAAVFTGLSVATRLYGFFFFLAILVYLILGLLRRIN
                     SLGGTIRKGVFFIFVMASVILFADPFLFRADAREKMVTILRQKSTEMAEGYVGDYDPR
                     NDYRTGWDAWYPAFEDHYTEMYCFFFLVFSLVFASFFGDQKLTHLMTLCWCVVIISYL
                     VFFVAVKSTQYVLPALLPLMASVFSIPQAVETGQVPAILRHKSIRIGSWIVAGGILVS
                     QFIINLIKIAPRF"
BASE COUNT         5379 a         4448 c         4251 g         5454 t
ORIGIN      
        1 tgataagccg ccattaataa ctaatttcaa gcaaaaatag ctgaaattgg tttagaatca
       61 aaaagtcgga gatcatttca gttgaatagt tttctaaaat acgcaaaacc ttataaaaaa
      121 tattttttgt ttggccctat atttatggtg atcgaagcga caggagacat tgtcctgcca
      181 tttttagtcg ctcaaattat caacaacggt gcggttaacc atgatatccc ttacattctg
      241 cgaaccggcg gattgatggt tggcttttcc gttataatgg ccgtttttgg cactttgggt
      301 gcaaaatttg ccgtcaacgg atcggttaac ctggctgccg atttacgctt tgacgtattc
      361 agcagaattc aggaattttc gttcaaaaac atcgatgatt tcagtacggg atctttgatt
      421 acccgcatca ccaatgatat tacccagatt cagactttcg caatgtcctt attgagaggg
      481 gttttccgtt ctccggtaat gttgatcggt gcggtgataa tggcttttcg tcttaattca
      541 caattggcga tcgtcatatt aatcgttata ccggttctcg gatcggcaat tttcacgatt
      601 atcaccaaag cctccccccg attcacaacg atgcaagagc aattagataa gcttaattcg
      661 gatatcagcg aatcgctaac gaacatcaga gttatcaagt ccttcgttcg ggaagattac
      721 gaaaataaag atttttccgt tgtcaacgaa ggtttacgtg aaaaaagtat tgccgcaatg
      781 aaaatggtca tcctccaaca gccggtcatt accgcaacta tgaatatcac aaccttagcg
      841 gttgtttggt ttgccggacg acagatcatc gtgggcagta tgccgatcgg gaatctggca
      901 gcgttcatca cctatattac tcaaatcctg atgtcactga acttacttgc caatatcttt
      961 ttgcaagggt cgcgggcaat cgcttcttca aaaagaatca atcaggtgat ggaaacaacg
     1021 attgaactgg atgacagcca tgctgccttc ccggaagcaa tggtggaaaa aggggaaatt
     1081 gaattcagac atgtctcctt ccgttatttc aaaaccaatc acgaaaatgt tttggatgat
     1141 atcaacctgg ctatttcagc cggggaaacc gtcgggatga tcggctctac cggaagcgga
     1201 aaaaccacga tggttcagtt gattccgcgc ctttacgatg ccgatgaagg tgaagttctg
     1261 gtggatggga tcaacgtcaa agattattca ctgaaaaaac tacgggaggg cgtcgcgttg
     1321 gtccttcaaa agaatacgct gttttccggc acgattgccg aaaatttaca atgggggaat
     1381 gaatatgcca cggaagaaga gatgagagcg gccgcaagaa tcgcacaagc cgaatctttc
     1441 atcgattcgc ttcccgacgg gtttgaaacc gatttgggac aaggaggagt caatctttcg
     1501 ggcggacaga agcaacgttt atgtattgcc cgcgctttat tgaagaaacc aaaaatactg
     1561 atccttgatg acagtaccag cgcagtggat actgcaaccg aagctaaaat ccgcaaagcg
     1621 ttttccgaga atcttcaagg aattaccaaa attattattg cccaacggat caattccgtg
     1681 atcgacgcgg acaaaattgt cgtgctcaac gaggggaaaa ttgtcggttg cggtgttcac
     1741 caggatctga ttaaaacctg tgttccgtat caagaaattt attattcgca aaaagagaaa
     1801 aacaaagagg gcgaataatg aatacacagc gaatggaaag actggcaaag aaatatggga
     1861 acaccaacca caatggtgtt gctcaaccca agacctttac gataaacacc atgtctgcac
     1921 tccgtgcgaa atccttatcc tcaaatgata agccaaaaaa tatgggctat accatcaagc
     1981 ggttacttga ttatctggaa aatgataaga aattgctgat tactgcgatt gcttgcattt
     2041 tatttcagac gatagccacc cttgcgggtt cgtacatgat ccgtccgatc atcaatacct
     2101 acataacccc gaagaacgga cagggggatc ttcatgggct ggcaaattcc ttagtcattc
     2161 tggctttcat ttatttgtcc ggcgttattt tacagtattt acagctgcgg attatggtgg
     2221 gcgtcagtca acgcagcttg gttcgattaa ggcgagattt atacaataag cttttaaacc
     2281 tgccgattcg attttttgat accaactcca gcggagatat catgtcacga ttcaccaatg
     2341 atgtcgacgc tgtcggtgaa atgctcaata cgacaatcgt ccaactggtt gcggggacca
     2401 tcacacttat cggtactttg ggattgatga tctacacaaa tatttggctg actctcatta
     2461 ccttggttat gattccgatt tttacctatg caagcaggtc cattattcaa ttcagccgga
     2521 aaccgtacag tcaacagcag accgcattgg gaatcttgga tggtttcgca caagaaacga
     2581 tcaacggcca aaaggtggtt aaggtcttca cccgtgaaga gatcgtcaag gatgaattca
     2641 aatatttaaa tgatgattta cgccgaaaac agatcgtagc tcaatttcgt tcctcaatca
     2701 tgggaccggt tactcataac ttgagcaact ttagctacgc tttgacgacc ggcatcggat
     2761 ccattttatg tattacgcaa ggatttgata tcggaggttt gacggttttt gtgaactaca
     2821 ctcgtcagtt cagccgtccg atcaatgaaa tctcgatgca aatgaacacc gtcttcgccg
     2881 cattggcggg ggcagaaagg gtattcgagt tgttggatga gccgccggaa ccgccggatg
     2941 cccccgattg tgtcaacgtt gatcatctgg ccggacatgt tgtgatgagt cacgtcaatt
     3001 tcgggtatta cccggacaaa cttgtccttc acgatatttc tctttatgca aaacccggac
     3061 aaaaaatcgc ttttgtcggt tccaccggtg ccggaaagac cacaataacc aaccttctta
     3121 ctcgttttta tgatatccaa tccggagaaa tcacgatcga cgggatcccg atcgataaaa
     3181 tccagcgtaa gatattacgt cgaaacattg cgatggttct tcaggatacc cacctgttta
     3241 ccggaaccgt gcgcgagaat atccgatacg gtcggttgga cgcaaccgac gacgatgtaa
     3301 tcaccgccgc aaaagttgcc aatgcccatt cctttatcat gcatctcgag aaaggatatg
     3361 atacgatgct cgaaaatgac ggagctaacc ttagccaagg acaaagacaa ttactgaaca
     3421 ttgccagagc ttccatctcc aaagcgccaa tcctgatact ggacgaagca accagctcag
     3481 tcgacaccag aacggaacgc catatcgaaa agggaatgga tgcattaatg tccgatcgca
     3541 caaccttcgt gatcgctcac cggctttcca cagtccgaaa tgccaatgcc atcatggttc
     3601 tggaaaaagg agaaatcatc gaacgaggag atcacgatga tctgctacaa caaaggggac
     3661 gatattacga actttattcc gaattggcgg aattggatta atggattttt tcaatgctgt
     3721 tatgccgaaa cgcagacttt cggcatagaa aaatcggttc ggaaataatt tcgtttataa
     3781 tatgtgttac aaaattcgtg ataccgataa aaaaacggaa tatttaacgg gatatgagtc
     3841 atttatcttg tcaacaataa aaaagactcg tataatgatt aaataaattg ttttttggag
     3901 aaattacaaa gtgatggaat caaacgcatc ccaaaacggg caaggggcaa cttctgaacc
     3961 ctttgacaac atggaaaccc ttctcgagtc gaagggactt aatatcgatt ttccccaaca
     4021 gggtgaaatt cgtcagggag ttatcgcaag cattacaccg gatcagattc tggtaaatgt
     4081 cggaacaaag tccgaaggga ttatttccgg aaaagaactg gaagcaattt caaaatcgga
     4141 acgcgatagc ttgaatgtgg atcaaaaaat cgccgtttac attcttgatc ctgaagattc
     4201 caacgggaat ctggtccttt cctacacacg agcacgggaa gaagaaagtt ggaagcgagc
     4261 ggaacaattg cttaattcca aagaaccgtt cgagacaaaa attgtcggct ataacaaagg
     4321 cggcttaatt gtcgaattgg gttcacttcg cggttttatt cctgcttctc aaatcagttt
     4381 gtccagacgg gtcgatgtca gcggggaaac acccgaacaa cgatggggga agatggtcaa
     4441 tcaaccgatt aaagtttccg tgattgaagt ggatcgcgaa cgaaggcgtt tgatttgctc
     4501 cgagcgggca gccagtaccg agacccgcga atctctgaaa gaacatgtta tcgaatcttt
     4561 gaaagaaggg gatatccgga ccggtcgggt tgccagtctg gcggatttcg gcgcatttat
     4621 taatatcagc ggagcggacg gacttgttca cctttccgaa atatcgtggg atcatattaa
     4681 acatcccagt gaggttttgc aggtcggaca ggaagtaaag gtaaaggtca tcagtatcga
     4741 taaggaaaag aaacgaatcg gtttgtctat tcgtcaaact caggaagatc cgtgggccga
     4801 acgggtggca aaatatcagg tcgggcagct ggtcgaaggg acaatcactc gattgacaaa
     4861 attcggtgcc tttgcaaaac tggatgataa tatcgaaggt ctaatccata tttcggaaat
     4921 cagtgaaaaa cggattgacc acgccaaaga agtgctgcat gaaggtgaga aaatcacttt
     4981 acgcgtgatc aaaattgacc cggccaatca tcggatcggt ttatccgttc gacgggtaga
     5041 ttccatggca tatgcagata tggattggga atcactattg gacagtgatc ttgaagcggt
     5101 cgagaaatca accgaagacg aagaaaaata agccgaaaag ctgaacaata caggctgtct
     5161 caaagataac gggacagcct ttttatttaa ctttttatat aaatcggatg aatcgcaaag
     5221 aacgaatgga aaaagttgta aaactttaat aaaatgcaac aattaaaaaa cggtaaaatt
     5281 atctttagta aattttatga gagaaccgaa aaggattcct ttcagcgggt ttgtgaaatt
     5341 taatgaaaaa tcagactttt tatgtcctcg cgttatttga tgaaaaaaca aatcaaattt
     5401 tcaccgaatt aacagaaaac ctgaaaaaaa ataacattga ctttcttgag gaactgcccc
     5461 acataacatt aggcggatac acggaagttc ccgaaagcga aattacctct tgggttaagg
     5521 aattttctga aactcactgc ccattgaaaa taaatttcaa tcatatcgga ttattccccc
     5581 aaagtatctg ttttatcgga tcaaaggtga acgaggagtt gttacgtttt caccgagaat
     5641 ttcatgccaa ttttgacagt tgtttcaccg agattggcta taacttcact ttgtgttcag
     5701 agaattgggt cccgcacatg acgataatca ccggaaatgc ggagaatatt ttcaaaggaa
     5761 tcccgatttt gttttccgct tttcagccgt ttatcgggac aatcacagaa ctggcaatct
     5821 atcaattcga tccttttctc gaacttcgaa ggttcccgtt aaaacacact gatttccaaa
     5881 cagacaaaaa aagatgaaac tttcgggcaa aacaaaggaa aatttatgaa ctttattatt
     5941 gacggtcatg aagatttagc atttgaagca ttggcaaatc agcgggatac aacgcgttcc
     6001 gtagctgaaa tcagagaaaa agaaccgcaa aataccgaaa atcaggtgac gctcggctgg
     6061 cccgaaatgc aagcggggaa agtagggatt atttttgcca cgatcttcat cgaaccgcct
     6121 gcacatcaaa cgcctcccaa acggacggga gtcaattatg gcacaccgga tgaatttcat
     6181 cagtccgttc ggaaacaact cgatttttat gaacaatggg aagattcctg tcccgataaa
     6241 ttcacgatca tccgatctct ttcggatctt gatcaaacaa tttcccgttg gcagcgagaa
     6301 gttgaaaatc cgaccggctc gacgttaccg gtcgggttgg ttcttttact ggaaggggcg
     6361 gaaggtttgc gcaaagaaga cgatctcgat gaatattatg atcggggttt gagaatcgtc
     6421 ggtccggtgt gggaaggcgg ccgttggtgc ggcgggactt attctcccga ttcgatgcgt
     6481 tttactccgg aaggaaaaag gttccttgaa attcttgcat ccaagggatt tattttggat
     6541 gtcgctcata tgaacacttt cagtgccgat gaagcccttg atctttatga aggaaccgtg
     6601 atcgccagcc attgtaattg tcgccgatta ctgaaatcac cggaaaatca gcgccatttt
     6661 gatgacgaaa ccattcgaaa attaatcgat cgcgatgggg taatgggtgt gatcccgttt
     6721 aacaattttc tggataccgg ttggaaaaat ggcatgccac ggaatcgcgt gacgctggat
     6781 cttttagcac aacatattga tcatatctgc caattggcgg ggaatgcgga tcatgcggca
     6841 atcggctctg atttggatgg cggcttcggc tatcccaata ttccggaaga gatgaatgat
     6901 attcaagatt tgcagaaatt ggaacagatt ttgcagcaaa aaggatatac accggaggat
     6961 ataatgaaga tattcaacgg gaactggatc agtgttttaa aaagaggttt accatgacag
     7021 aacagaacaa cgagataccg ggaaatgaga cacaaagcga agaaatcaga gcgcaaaaaa
     7081 ggctgcgaaa cgcccttttt gtaactttag gcggttacct gatccttgtt ctgggcgcca
     7141 aaccatccat tttcggattg gatcgcagtc cggtcatcgg atttgtgcaa acagccactt
     7201 ttattgtcgg actcgggatt atctgtctcg gcggccggat gagcttacga agcttttgga
     7261 agggcaaaaa gatttcattg acagccgatt ttgggctgcg cgtggtcgcg accgggtatg
     7321 tgatcgcaat atttaccgca atggctgatg tgttcggttt cggttcgcac ccactaccgg
     7381 gagtaccgtt cttcggtccg ttgcaagccc gcggcgtcat tttgggagaa gcgattatcg
     7441 cagtcgggtt gatcatgatg ttttctcccg tcgatgaaac agaagaaaaa tcaaaagacc
     7501 ggcaatcaaa ttcggaaaaa agcgcttagt atttaccgta aatcatagat cttaaaattt
     7561 gaaaaactta cgctgatcgg agaaactcct tgggaattag ccaataagcc gatagcaccg
     7621 ctttttaaaa gggagtcttt cgtctcgtac aaaaagacat catttataaa aaatcttaat
     7681 gtttcacctt tgattcgcat gcctaaggta aatttttgcg gtccgccaga gcgaatttgt
     7741 ccgttgacgc tccaatccgc caaaggcaac atctttccgc cgtatctttg ttccacccgt
     7801 gtctgtccct gacaattaat cagaaagcga tcataattat ctttatcgcc ataacggaag
     7861 aatactccgt aataatccga actgtcagaa caaatcggca aagtcacgtc gacactcaaa
     7921 taataatccg tcttgggatg atagtctaaa aaggaagcga tcgaaccggc cgagaattga
     7981 atcgcaagtg tcaattcatt gttcgaaatg acggcatttc cctgatcaga tcgaaaagtt
     8041 tgccaaaagt tttcattgag gaatacatct tccagtaaaa cttccccgac atcgggtttc
     8101 atatcctttg tggggaaggc ggtcggtgtg ctgagcgggc ggggtgtggc agttgcgggg
     8161 aaccattgga cagtcaccgt cggagaatcg attgccgtcg gaaccagcgt tttctgattt
     8221 tgaaaggtca tcgtttcggt cggttccgtt aaggaataaa tattgacctg atcacaacct
     8281 gtcatgacag agaagagcat catccaaaag agcaatttga aactcaacct tcgcatcatc
     8341 atcgatcaca ctttcaggtg cgcaaccgta acgccttctc cgccttcatt atcttttccg
     8401 ttctcgatat attttacata aggagaatcc aacagaaatt gccgggcagc ttgccgcaat
     8461 tttcccgtcc cctttccgtg gataattcga acataaggaa gtccggctaa aacagcctca
     8521 tccagatatt tttccaatag atcggtcatt tcctcggcgg tttttccgcg gagatctatt
     8581 tccacacccg gagagggatg gaaaatttca ccgcttcctt ttcgctcttc cattttaacc
     8641 ggggattccg tctttccggt cggtcctccg gtattttcat cgttattccc cgtatcatcg
     8701 gatttccgaa tcaaatcttt tatcggaacc cgcattcgca gcgctcccaa cagaatttcg
     8761 gcttcgtctc cgtctattgt tgtgatcgta gctaaggaat tcgttcctaa cgaacgaacc
     8821 cggacttttt caccgatctt cagcggagcc gcatggtaag tcttcaattt gcttttcttt
     8881 tcgatatggt tctcgataga ttgagcggct ctttcacttt cttttaaccc ttcttcaagc
     8941 ggtttcaact cgccgagagg tattttggct gccgcataag ctttgcgcaa tttggcaagc
     9001 tccgttttta gttctttaat ttcctcttcg gtttcgctgt gtgcttcgct taggattttt
     9061 tctttttctg cttcgatctc ttccaactgt tttcgcaatt tgcgttcatc attttctgct
     9121 aaactgcgta accgatccgc ttgcgatcgt gcttttcgag ccgcctcctg ttgacgatga
     9181 atttcgtcca acaacgctcc ggtatcctga acttcgggat cgatcgtttc acgagcatcc
     9241 tctaaaattt cggcaggcaa tcccaacttc tcggcgatta ataaagcgtt tgaacgtccg
     9301 ggaagtccga tactgagtcg ataggtaggc cttaatgttt tgagatcaaa ttccatcgat
     9361 gcgttgatcg cccccggagt cacatgagca aaagtcttga gttcggaaaa atgagtcgcg
     9421 acaaagcacg gaatatgctt ttttatcaga tactgcagga tcgcccgcgc taaagccgaa
     9481 ccttcctgag gatcggttcc cgaccccagt tcatccagca aaaccaatgt tttggaatcg
     9541 gcttctttca aaattcgaat aatattggta atatgccccg aaaaagtgga gagcgattgt
     9601 tcgatcgatt gctcatctcc gatatccgca aagacattct tgaaaaccga gaattccgaa
     9661 ccggatcgga cgggaatatg caaaccggat tgtgccatca ggatcagcag cccgattgtt
     9721 ttgagggata cggtcttacc gccggtattc ggtcccgtta tgacaaccgc aaacgtttct
     9781 tcgtccggta caacgtcaat cggaaccgcg gtttctaccg gtaaaagagg atgccgtgcc
     9841 tgatagagtt tgatgaccgg attccctgtt tttttatagg gatcaaccaa taacggttcc
     9901 gccgcccgca aatcatcggc atatttggca cacatcatgg caaaatcaat ttccgccaac
     9961 gcttcaaccg attgttcgat ctgatcggca tagtcaccga caaaagcggt caaattctgc
    10021 aaaatccgga tgatttcatt tcgttcatcc agttgtaatg cctgataaac attattcaat
    10081 tcgactgtcg ccagcggttc cacaaacaag gtggctccgc ttgaggattg atcatgaata
    10141 atcgatttca gttgcccttt atattcggct ttaagaggaa tgacatatcg cccatttcgt
    10201 tgggtaatga tcggttcctg aagcatcggt tgatagcggg attccttgac cattttctca
    10261 agtctgccca acaatcggtt ataagaaagg cgaatctcac gccttatcga cgtcaatttc
    10321 tccgaagcgc tgtctaatac ttctccccga tcattgaccg tgaaattaat ccgttcgatt
    10381 aaaccagccg gaggaatcaa gttttctgcg atcgcagcta aattgggata ttccgcttca
    10441 tgatgggaca gggaacgata aagatctctg gcgaccgaaa gcgtataagc aatttccaca
    10501 aaagctttcg cttctaaagt tccctgtttc cgggcgactt ccaagtacgg acgtaaatca
    10561 ctgcaaccgc cgaggcgaaa ggtgtcgttg acacttaaca attttcgagc ttcggttgtc
    10621 agactgagct tatagcgaat gatcgaagaa tccgtttcgg gatgaaggtt ttgtgccatt
    10681 tgttccgaaa cattaaagct tgcatattgc gcaagtcttc gaatgacttc cggatattcc
    10741 agcatcttga gtgatttttt gtccatttta tgcacttatc aattgctata cgagttcacc
    10801 gatcatcgtc cagggtcccg tccaattaat ttttaccggt aaaatcagac ctgtcaaatc
    10861 cgattcgctt tcgacataga ccaagtccat atgctctgtt cttcccatcc aacggccttt
    10921 ttgtttttct tcaaacagca caggaagaat tttcccttcc aaggccgcgt gaagatcggt
    10981 cgcaatccgt tcctgcaacg tctcaacggc ccgaaaacgc cgccattttt cttcatcgga
    11041 gacatcatcc ggcatcgtcc gctccgataa agtgcctttt cgcggagaat aacgggcaag
    11101 atgtaccaca ttcagtttca gatccgcaag ttgtcggaac gtatcttcaa attgcgcttc
    11161 tgtttctccg ggaaacccga caataatatc cgtcccgatc gagacccccg gtatcttttc
    11221 gcggatatga gaaatcaaat tacgatactc ttcatccgta taaccgcgac gcatattttc
    11281 aagcaccgta ttattgcccg cttgtgccgg aacttcaata tgtttcatga ccttcggtaa
    11341 ctcgcgcacc atatccaaga gttcatccgt tatccaattc gggtggctgg tcaaaaaccg
    11401 aattcgttcg atgccgggga tttcatgtat ttcccgcaac agttcagcca aggtcggata
    11461 accggctgta tccaatccgt aacggtctac gatttgcccc aataaggtga cttctttcac
    11521 accttgctca gccaacgaac gcgcgtggtc aagtatttct tgtggattgc ggcttctttc
    11581 cgctccacgc ttatatggaa tgatacagta agcacaggca tgagaacaac cgtaaacaat
    11641 cggaataaag ccggcgaccg ttttttttcg gcggttagcc ggcaaagtca atttgcccgg
    11701 ttcccaaacc gtatccgttt caacatttcg ggaagctgat gcttcaccga tatactcctg
    11761 ccgaatcaaa aaatcgataa tcggcttcgg attggagggc ggcgcaaaaa catcgacata
    11821 aggcaatttt ttgcgaatct ccgtctgatc tttaaatccg accatacaac ccattacgcc
    11881 caacaccaga tccggatttt tctttttgag tggtttcagc gagctgagcc tgccgatcgc
    11941 tttatcttcg gcactttgcc gaaccatgca ggtattcagg acaatcacat ccgcttcttc
    12001 gatgatcggt gattcgctat accccaaacg ttccaatgcg gatgccaatc gctgagagtc
    12061 agccacattc atctggcaac cttcagtcca aatataatat ttcatcattc aatcatactt
    12121 tcatacattc aaacgatccg gaaaaggaac agccattccc aagaattccc atccgaatac
    12181 ggttttattc tttcgattcg ttccgatttt tatcttcgta aactataaac aagctgtcaa
    12241 ccttttttct caatcaccca aaaatgggaa aggcccagcg ctttcaaggg agttctttct
    12301 aaaaaaagca agatttttcg cagcatcagt cccaatccct ttgaacgggc aataatatta
    12361 tcataagcgc cgaaaatgac cgtcctggaa atgacttcga ccggaagacc attcaatttg
    12421 cgctgcaaat ccttggtcgt atatacgttt acatgcggtg ccagtttatc tcttaatttt
    12481 cgcggtaagt aattgacccc aaacttattt ccgaaatggt atttcccttt ccaataaatt
    12541 ccgtgagttt caaaaggata ccaacggttg gggcaaaaca aaatcaacct tcctcccggc
    12601 ttcaggcaac gaactatttc tcgcaggcag gcaacatcat catccacatg ttccaatact
    12661 tcatgactca atatcagatc aaaagtatct tccggaaacg gcagctcttc tcctacggca
    12721 tttatgacgg atgatcgttc gccaccgacc aggtttaaag cctcccgtgc ccgatcgaat
    12781 tccacttcca agccaacgct gaaagttgcg acgggagcca atcgttttag ataagccccg
    12841 acaccgcaac cgttttccag aacaaccccg ctgtctcgac cttcggcagt tgtttggatc
    12901 attttcaatc tgcgtttttg gccatccctc caaacgtaag atggctctcc tcgttcggct
    12961 gcctgtgcgg aaattttctg tgtatgatcg tcgttcccca agaatttatc cttctttcgc
    13021 cgttttcgga agccaaactt ccagtttcgt acccttgccg ggtttggatg tcatttccaa
    13081 ttttccgccc agattctccg cccgggcaaa catattgttt ataccatggc tctccggttt
    13141 tcgccctttc gccggatcaa atccactccc gttgtcctga acaatcaaaa catatcgatc
    13201 cggttcttcc gtaaaagtta tcatcacctg ggtcgctttg gcatgcttgg taatattcgc
    13261 caacgcttct ttgcagatca tatagaaagc attcgcataa tcctcggaaa tttgatccaa
    13321 tgtctcaacc ttgggatcaa aaacagtcgt caccaaggta ttcgaataaa aatcattccc
    13381 caaccggcgc aaactttgaa gcaaattttc accggtcaag cgtgccggtt taagatccat
    13441 aatataggac cggatatccc ggatcgtcgc gtttaatgca tcgatggatg cctgaatttg
    13501 ttcctcgact ttttccggat ccgcttttgc caaaatgcgg gcatgctcca gcgtcaaccc
    13561 cacaccgtaa atagattgaa tgacaccgtc atgtaaatcc atgctgatgc gttcccgttc
    13621 ttccagaatg gcaacccgtt ttctttgcaa cgttaaacga tagtcttgaa tcaaagttgc
    13681 catccaactg ctgatcgaag acaaaaactg aagatttaac gtatcttttt catccaccaa
    13741 ttttgtaccg agacaagaga ctcccacaac cccttcgctt gtaataagag gaaggaccgc
    13801 aacatagttt aaatcaactg ttgtcgctct tttctggttg ataatttcca gttcttcctc
    13861 cgaaagcaaa taattccgct tagattgtgc cacttttccg acaattccct gtcccatctg
    13921 aacagtcgaa aacccgaaca aactggtagg cagattgtgt ccttctttga atacaatttt
    13981 gaactggttg ggagcgtcat catcccgtaa aaaaacttct ccgacaacca aatccagata
    14041 tcccataacc tgatgcatcg tctcgtgaac gatcgcatcc tggttttctg tggacgaagc
    14101 ggaaatcttt gccagttcat tcagtaaagc cagatcctca tttcgtttcg caagcatctg
    14161 ttcccgattg gcaattcgct cataaattat cgtattattg atcccaaacc cggcatatcg
    14221 cgccaaaatc tcgataaatt tttgatcgat cgaatcaaat tgaggggcac caatcttatt
    14281 cagcaaaata atcgacccca ttttctcctt gccataaaaa atagggacat acagtaagga
    14341 gctgataaaa ggtcttttcg cggggaaaat attgtaattt tcgggaaggt cttttcctct
    14401 ggcgatatta atgattgcat ccgaatggat attgcgaatc ggctgaccgg cagcttcctc
    14461 atcgctgtga atcaaatcca attcaacctg agtaaaaccg cgtaagacga tttttgtaaa
    14521 ggtatcttct tcatccgttg taccgcagat acaataggtc gcatttgtga ctgaaattgc
    14581 aatctccgcc gttcgattca acaacgattc aatcgaaatt tcgttgagca aattcaggca
    14641 atcctgataa aacgaatcaa ttttcgggat tatttcccct acaccggctg tcttcatatt
    14701 caatttattt taccttgaac ctatctaaaa cccgactcaa attgtcgtaa atcctcctta
    14761 tactgtacgg tttatcaatc cgatttgttc atttcattga ttttctcttt ttattattca
    14821 actttaatat taaagtaacg gattctctac tataattatg aaaaagaagc atttccgatc
    14881 ttaataacgg atcgagaata aaggaagaaa taagtggcga aaaagcagga aatagaaaat
    14941 aatagtttca caatccggga tatcactgac gttacacccg ctcagagaat tgcggtattg
    15001 attgacggtg ataatgcaca agcctctttg attgacaaaa ttcttgcgga aataaccaaa
    15061 tacggattaa tcaccattcg acgaatctat ggggactgga cagcatccaa tatgagcggg
    15121 tggaaggatg ttttgcagac tcatgccatt caacctattc agcaatttcg ctacacaacc
    15181 ggtaaaaatg cgacggatag cgcgatgatc atcgatgcaa tggatatctt ttattcggga
    15241 acggtcgatg cgttttgcct ggtttcgagc gacagtgatt atacacgatt ggcaacccgc
    15301 attcgcgaac agggactttt tgttatggga atcggtaaga agaccacacc tcgtgccttt
    15361 gtcaacgcct gtgatctgtt tgtctatacc gagaacttgg tcccgcagaa tgaaccatta
    15421 aaaagcaccg gaacaaccgc caattcagcg cataagaaga aaaccgccaa atccgccgaa
    15481 tccagtccgg ctccggatgt cgaaaacggc aattcacccg acccgctgcc gttgttacgg
    15541 agtgcttttg accttgcgac ccgtgataac ggatgggctt ttctcggcac tctgggtcat
    15601 cacttacaac aattggaccc gggttttgat tcccgtactt tcggttatcg acagttatct
    15661 ttgttaattc aggcgtatcc ggatgtgatc gaaaccaagg aagtcaaggg agccgacgga
    15721 acttcgacga tttatgctcg tttgaagtca taaccggaaa gagaatatcg aaatggcatc
    15781 ctcttctttt ttatcttccg ttcgggcgac aatcgaaccg tgcaaaaaag gttttaagag
    15841 aaaatttccg atctggatgt ggcaatccgg tttggggaat gttctgaacg catttccttc
    15901 cttaaccggg gaacaagtgg ttgttatcct tccggacaat accaaattgg ctttaaattg
    15961 taccgaaaaa gacgatgtca tttatgcctt gctgccggct atggagaatc cggagatcta
    16021 ccggactttg gttgccaatc ccgccgtcca aatctggtca aaaaacggct ggtataacgc
    16081 cggtgcccgt ctgttgactt tcgaagaaac cgcgaaaact ttatcttcct ttcaaccgga
    16141 atcattcttc gggaaaatcg gcgcatcttt acgcaatccg gatcaagatg ctcgaatccg
    16201 tatgatcgaa atccggagaa tatcggcctg tacgggtgaa aaaggaccgg gacaatatgc
    16261 ctgggtatgg gctgcttcat ccctgttctt cttcttttct tgggcgcgtc aaagaaaaat
    16321 aaacaaacgg acagcccata aattaaaggt ctgttcgagt caatcgaata agaaaaagtg
    16381 ataagaaggt gaactgtgaa ttcttataac aataacacaa acttcgtttc atcgtctccc
    16441 gattccaatc ggaaacagcc cagacggtca ttcccctggt ttactgcgtt catcatgatc
    16501 ctcatcggat gcctcattgg ggttctcatc tctttaaatt ttgttccgga accgaagccg
    16561 gaaacatccg cttccgccga acagatcgag aatcttcaag cgacggtgac tgctttgcag
    16621 aatactttga agcaatctca accggccgca aatacactca atgtcagtta caccaatgtc
    16681 gaaacagaaa ttaccaatgt tgttgaaaaa gtaggacccg ccgtcgtaac ggttaccgct
    16741 aacatcccga ctacagccag ctattactgg tttcagacaa gctatgttgc gaccagtatg
    16801 ggcagcggtg tgattatcag tgatcaaggg tatatcctta ccaacaatca tgttgtggaa
    16861 ggggcaacgg aactgagcgt cgaactatca gacggaacca cgcttccggc aaaactcgtc
    16921 aataccgatg aatttacgga cttggcagtt ttgaaagtgg atggaactat gccgggtgtg
    16981 gcaaaattgg gcgaatccaa tctgctgaaa tccggagaaa cggttatcgc gatcggctca
    17041 ccgctgggga attttaagaa taccgttacc gtcggcgtgg tcagtgccac cggtcgtttt
    17101 cttgaaaacg ataaagggta taagatggaa aatctgattc agaccgatgc agcgatcaat
    17161 caaggaaatt ccggcggacc gttagtcaat ttagccggtg aagttatcgg aatcaacgtc
    17221 atgatcgttc gcggttccag tacctcgtcc acttatgcag aaggattagg ttttgccatc
    17281 ccgtccgata cagtcaggtt aattgcgcaa cagattattg aaaagggaaa acttaccaga
    17341 ccctatctgg gaattcagtg ggtggatatc actccgcgtt tggcggaaaa ttacggattg
    17401 cctgttaagt acggcgtcat cgttcttgcc atcgaaccgg gcagtcccgc ggatcaagcc
    17461 ggactaaagc ggaatgatat cattacaaaa atcggagatt atgaaatcac ggaaaacagc
    17521 gcatattata actgtctctt taaatactct ccgggagata aagttaccct cgaagtgatt
    17581 cgaaacggga agaagatcaa tatcgatgtg aaactcggag gagatacaac cgcattgtaa
    17641 aaatgatccg aatatgaaag atgcagctgg tcagcttagc cgaaaatcga ttcaaaaggt
    17701 aatgattata agcgcgatcg agggattggt cgcgcttttc ttcacttttc tgcatccgtc
    17761 tgaaaatgaa tcggctgttt ttgccggtta ttccgcgcaa agattcatct tgggcggaat
    17821 aacgctggtc ggcgtggcgc ttttcttgtt gatcggaaaa ttgttctcag agaaccaccc
    17881 ccgatcagaa cagagggtac aatcactgaa cgattttctc aggcaaaatg atcggatttt
    17941 cttttctctc gttattcttt cgggtttgac cctttttgct atttggtgtt ttgtattttc
    18001 atggcttttt atccccgcta atttgcgtcc gcaaattatt tgggtgggct taattgcttt
    18061 gcaaatcttg ctttgtatcc ggcgaagtta cggggagacc attcgtcaaa atcattatgc
    18121 cgaaaaatat cgattattcc cgcgttggaa agatctgtcc gcaaatcaaa agaaagtttt
    18181 tcgctttctt gtcattctat ccgtagctta tatcctgatt ttgataccgt caaatcttaa
    18241 cggatcgcag aacatggaag cgtttgcccg gtatggcggt gatgagtttg ttatttaccc
    18301 gatcttgatg gatatcatga aacccgggga gacattttcc gccacacttt accatttgtt
    18361 tatttatgaa gattatcatt acggctaccc tttttatgct tggtcaacgt tggttctgct
    18421 tccgataaag tggatttcgg gagaaaattt tgcggaacag attcagatta atttgccatt
    18481 acttcgaatt tttgtttcgg tgatcccggt cattttagcc tgcctggtcc tcaccttttt
    18541 atttacccgc tggaaaagtt tttggatcag cgctcccgtt tttctctttc ttttatcggc
    18601 accggggaca cttcaaaaca atcagggatt ttggcatccc gatggattga atttgctgtt
    18661 tgtttgtctg acactttatt ttctgcagcg ggatcgattc cgatacggtc gcaatttcta
    18721 tttggcggcg gttttcaccg gcctttccgt ggcaacccga ctctatggtt ttttcttttt
    18781 cctggcgatt ctggtttatc ttatcctcgg attactgcga aggataaaca gcctcggtgg
    18841 gacaattcgt aaaggggtgt tttttatttt cgtcatggca tccgtaattt tatttgcgga
    18901 tccgttcctg tttcgggctg atgcacggga aaaaatggtg acgattctcc ggcaaaaatc
    18961 taccgaaatg gcggagggat atgtcgggga ttatgatcca cgaaacgatt atcggacggg
    19021 atgggatgcc tggtatccgg cttttgaaga tcattatacg gaaatgtact gtttcttttt
    19081 cttggttttt tcgcttgtat ttgcttcctt tttcggagac caaaaattga ctcatttgat
    19141 gacgctctgt tggtgcgttg taattatttc ttatcttgtc ttttttgtgg cggttaaatc
    19201 cactcaatat gttttgccgg cattgttacc tttgatggct tctgttttca gcattcctca
    19261 agctgttgaa accggacaag tgcccgcgat tttacggcac aaatcgatcc gaatcggcag
    19321 ctggatagta gcgggcggga ttttggtttc gcaatttatc atcaatctga tcaaaatagc
    19381 tccccgtttt tagagtttat cgacaaagat ttccacagat tttttcagtt ttgttcacaa
    19441 tttttcggga attatccacg ttaatctata agatccggcc ttttgcgcat tttctccacc
    19501 gatttttcca caagttgtcc accggtcttc cg
//
LOCUS       DAOSMM010000042        19268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2848_length_19268_cov_16.204133, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000042 DAOSMM010000000
VERSION     DAOSMM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 19268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19268
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2848_length_19268_cov_16.204133"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(544..912)
                     /locus_tag="PK898_07050"
     CDS             complement(544..912)
                     /locus_tag="PK898_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU19827.1"
                     /translation="MYSNDLFSQYGLIVEKIIQSINESYKDIIIDKYVIMPNHMHMII
                     FITNDEKQSNILQHANERIPSLVSTLKRFSNKECGFSMWQRSYYDHVIRDQKDYENIW
                     QYIEDNPIKWLEDEYYRENE"
     assembly_gap    1693..1792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2024..2560)
                     /gene="def"
                     /locus_tag="PK898_07055"
     CDS             complement(2024..2560)
                     /gene="def"
                     /locus_tag="PK898_07055"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU19828.1"
                     /translation="MEVKQTGDPILRRKAHKITRIDSSIRKLASDMLETMRENNGVGL
                     AAPQIGESIRLIVVEYPENDEIEDSPLISYKLINPEITWMSEEKTIGVEGCLSFRGLA
                     GEVERAESVKIRALNIHGKPFNITANGWLARIFQHEIDHLDGICYVDRANKVWEMSAE
                     EEEETEQDESITNNPPIS"
     gene            complement(2642..3544)
                     /locus_tag="PK898_07060"
     CDS             complement(2642..3544)
                     /locus_tag="PK898_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19829.1"
                     /translation="MSLEPRESKENTGLPASHILRLLKGLGILAVCLLICWFLFGRYK
                     IIPIIAGISVVIFLFYCIYVFADLRIKPPVKADRRNRPPKPAVYYISEYTPTAYSYVK
                     QKKGNAAEKEGMVYRAHWAYFVRQAIIPILLLLCSLVTIVVGITRDWEIFEVFEIKAA
                     LLILVVVFIFWIIYALIDWSNDLYYVGAERVADINRKPFAKKEVTMTLISKIQSVQYK
                     KSGIFQYLFDYGTIKILSGESELLFVYVPHPDKVQLEIIKQMEANDAKKQLNDPEKQP
                     VIQNDVTDSPIHYFNRKAAKNRLE"
     gene            complement(3548..5986)
                     /locus_tag="PK898_07065"
     CDS             complement(3548..5986)
                     /locus_tag="PK898_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU19830.1"
                     /translation="MMRFDRFTESAQDAAQRAVEILQRYGQNQIDTEHFLLALIEQPQ
                     GTVSQILDEMNVSTAVLSDRLDYMLRTSTKTNIYGGGSGQIFITPNVRRIVDQAYIEA
                     NRLKDEYTSTEHIFLAILNEKGTPVAKLMEEFGITRAKVYEIVVKMHDNKNAPVQNKD
                     SRYKNLQKYSRDLTDQAREGKLDPVIGRNVEILRLMQILSRRTKNNPVLIGEAGVGKT
                     AIVEGLAQKIVANDVPELLSGKKVISLDMGALVAGSKFRGEFEERLKAIIDEVKKSNG
                     EVILFIDELHTVVGAGASQGAVDASNMLKPALSRGELQCIGATTLDEYRKYIEKDTAL
                     ERRFAPIYVEEPSENSTIEMLHGLKDKYEQHHKVIYKDDALQAAVRLSSRYVTDRKFP
                     DKAIDLIDEAGAKLRVALYSLPPDLKEMKNEIDRMAKEEEDAGLERDYEKAAKIKAER
                     LRVEDEFNQKKATWESDHKLDEVVDVDDIAQVIAQWTGIPVNQMLESESEKLLHMEDR
                     LHEKIIGQDEAIHAIADAIRRARSGLKDPKRPIGSFIFIGPSGVGKTELAKALAEYLF
                     DDEDALVRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVILFDEIEK
                     AHPEVWNTLLQILDDGRLTDGQGRVVDFRNTVLIMTSNLGTEYINRGGTLGFIGSKDE
                     AEDREAKLKVDKALKSTFRPEFLNRIDEIIMFSKLSLDDMKKIVDIQMEEVRQRLSEN
                     GIEFELDDDARQWLATIGYDQAFGARPLKRALQKYVESPISTKYLGGELKRGDKIQIK
                     KKADAEELEFLKQI"
     gene            complement(6156..6857)
                     /locus_tag="PK898_07070"
     CDS             complement(6156..6857)
                     /locus_tag="PK898_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19831.1"
                     /translation="MDPEVFLDLYNNLETKLKGRGSYYNDRYESPIIRFSNSSEGKPY
                     REELSTIRDIRNLLVHSPLVNGKYPVEPSDEIGAVLEEILEKVEQPKKAMNYATWADK
                     IQKTRLDEEAYPVMRMMELRGFSHVPVVDGEKMLGVFSKSTVFSFHIRNNMPALQRGM
                     RIREFGNLLDIENHSNEYFEFAPKDALYTDILELFNTNYKKRQKRLAIVFLTETGNSQ
                     EKLLGLITPWDVLGK"
     gene            7045..7719
                     /locus_tag="PK898_07075"
     CDS             7045..7719
                     /locus_tag="PK898_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19832.1"
                     /translation="MSKKQKNNKNSKQNAGDLFGLALWIFAAFGLEIVLIFIESKIYA
                     VSSLNWTIRQNCLHWALISLFWAIISFLIVRSAKKDYDFDIFKRQQNPTMTQILFSVL
                     LVIVGILFVSYMSGGFKPVSEYKKLGSVQFIFQYITYFFKSILVMLAIALGQKAGDLY
                     FQKDQIPWGGYFLAVSWGFIQFIIQNPATGLFTVALSVLFGVLFMINHKNARIVFPMI
                     AIVLML"
     gene            8055..9185
                     /locus_tag="PK898_07080"
     CDS             8055..9185
                     /locus_tag="PK898_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOU19833.1"
                     /translation="MKKSALLAIVVLCLAVSTGVLGFMYSTKGDKTAEEAAPMVQIGV
                     MTNKWAASAHGDSSSIAFRHWDEEGEVPVTCAKCHSSTGMQDYLGADGTAAMTVDKPA
                     KVGSVVDCSACHNEVIQNTTSVVFPSGKEVAAEPSYASCWTCHQGMEAGINGSLDQKI
                     GSTAPDDKGENLGFVNPHYLNVAGIYLGSEANAGYQYEGKTYAARFEHASGVQTCTEC
                     HDPHSLHLADPGLEKCSTCHAEVETWPDQQKIRRTKEDLDGDGNTDESTYEEINGLLE
                     RELQAMVTYSVDVAKVQIGNSDVYPYWFADKNGNGTQEEDEKQYADWTPRLMYAAYNF
                     KLVKTSAGYIHNPTYSAQLLIDSISDLAAGDSAISVDGIERP"
     gene            9204..10577
                     /locus_tag="PK898_07085"
     CDS             9204..10577
                     /locus_tag="PK898_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOU19834.1"
                     /translation="MNTAASRSARRAYIAQLRVKRIFWICLFVVIALFPLVVAAINNT
                     TNGTWRAVPREIGSFAGMLGLSLMILSFIPIMREKKITSAFNLDVVYKFHHSCSILGF
                     WLVVFHLVALWINNQEIGRLVNIFADYPLYMKAGPIALLAAGCLVYFSYFRKELKLNY
                     DYWKIGHSLVAILMVVFGLIHTFGVNYYSGNALIHGYYVFLTVLAAIAILWLRVFSSL
                     SAMCKPYVIREVLIRNNATTELRLEFAGKGNQKPIRYHAGQIAWITVRHSRFSFKEHP
                     FSIASSDIDQNKIGFAIRELGDFTATIKDLKPGEIVYVEGGFGIFNPYILGDEGFVLI
                     AGGIGIAPVMGILRSMADRKDRRHVVLYYGSRDKESIGFYDELNELSKKLNLEVVHVL
                     EKTDDPSFEKGYVTKDVIERHYPKNKDRYDVFICGPGPMVQIVLPALKKIGIPKENIW
                     PEFYDMA"
     gene            10706..12193
                     /locus_tag="PK898_07090"
     CDS             10706..12193
                     /locus_tag="PK898_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU19835.1"
                     /translation="MDQYDIIIIGGGVVGCMIARELSRYSPKVLLIEKTSDVGCGTSA
                     ANSALIHAGYDPLPGTIKAALNVAGNRMWDSLASELDFDFTRCGDYVVAIGDEELPAL
                     DRLKSQGVQNGVSGLKTFSAEEVRKRIPRINPEVSGALFAESAGICDTFAVTIAAAEN
                     ALHNGVEIRLNTEFKGFIIEKNVIRGIHTDRGDFGCQWVINAAGLYADEVMHQANCRL
                     DFKITPRRGEYCILDPDLFRIDTVIFPVPSEKGKGVLVFNTTHGNTVVGPTSEFVSDK
                     EDKSISPEGLNDLLKNVRKLIPSVDLRWTIATFAGLRATGNAPCPDPAIHYDSDFIVE
                     SAQEIHGLINCAGIESPGLTASPAIALRVTELLKEEGVRLTPNPKWDPIRKRRPSPKH
                     LSESERQKLIEADNRYGRIICRCEGITEGEIVAEIHAPIPARTYDAIKRRTWTGTGRC
                     QGGFDMSRVVNILARELKVSPLEVRKNDPGSEFLFRESKAIGGTL"
     gene            12190..13461
                     /locus_tag="PK898_07095"
     CDS             12190..13461
                     /locus_tag="PK898_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU19836.1"
                     /translation="MNLRSEYDVIVIGAGPAGLAAAIAARQNGCDQVLLIDRDVEPGG
                     ILQQCVHNGFGLETFKEDLPGPSYAQRFINRAIDCGVQFLMDTMVLAVSRDKQVTVSG
                     ALVGIRTLQAKAVIFSMGCRERSRAQIRIPGTRPAGVYTAGTAQRWVNIDGLMPGNRF
                     IILGSGDIGMIMARRLTLEGAQVERVVEIMPYLSGLSRNLVQCLQDFEIPLFLNQTVS
                     RIIGKKRVEAVEISAVDEKRLPIPSSAEIIPCDTLLLSVGLIPENELTRRAGVEMDER
                     IGGAVVDDTFSTGIPGMFVAGNVVHVYDLVDYVSQAGEKAGSSAADYIRGKTSYSEKK
                     VKTTAGKNVRYVVPQLIRPESLKETSILLQMRVVAPLEHRVELIVRDGEKEITRKRLP
                     YARPGEMVSVSLPQSAYDIVQQADEITVEIL"
     gene            13471..13845
                     /locus_tag="PK898_07100"
     CDS             13471..13845
                     /locus_tag="PK898_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOU19837.1"
                     /translation="MIEKRELICVTCPRGCRITVEMEDEKIIRILGNTCERGKKYAES
                     EIKDPRRMFASTVRIRNGVHPVLPVTVTQPFPKDRLKDLSAELRKTEVTAPVRMGDVI
                     VKNVLGTGIDVIASRDMESVRS"
     gene            13938..16070
                     /locus_tag="PK898_07105"
     CDS             13938..16070
                     /locus_tag="PK898_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU19838.1"
                     /translation="MEDKTAEKKIYSSGYAGTIPGVHKEINEDFVIFFEKEPEGTVGA
                     SNLYVLTDGVTGTFHPEITARFAGKKILYEYFKSADFIDANKLALAMRNANNEIYAYA
                     ELKQEKMATVALATAITDGNAVIGTIGDSRVYVVRNDKVYQITEDQNILEEKVRKGEL
                     TKEEAFETEAHKPVIRALGSEKDIVVDIYDGIEVKPGDVLLMCSDGLTAYIGKDEILE
                     AVQADTPKEIVRNLLAYAANQNSPADASAIAIKIYDENTIQTVVRQPGTLPVDTDLNK
                     ETKELDLMMKSKPRHQPEKTEKKGTKKTSAYIISALLICLVIGGIFWFFSDSEGRPSL
                     FGPRMTATATVDPVQATLAIIHQTQQAEAIARLSATPTATLTPMPTMTSTVTVVPTLF
                     DLNVPVAGAVTEAAEFGGTQTVLTPGTAEVQVTITPTPVDKGPIVSEKDQAEMVYVKA
                     GEFLLGSDPLKDGYANTIEETPQITVYLDGFWIDKYEVTNARYLKCVQAGGCNQSSYM
                     LLNDPTYADMPVTYVTVEEAEQFCEWAGKRLPTEIEWEKAARGTDGRIYPWGDEEPVE
                     GTRYANYPGYTDPDAQQVSGLFKVGSFPEGASPYGAMDMAGNVWEWTSSKYQADYYQT
                     LVDEAEMGTEVIRNPKGSQSGIANVIRGGSAAETEINNYLAYLRTANRSYVNMNSSYY
                     IGFRCVLPDSESTGETGAVVQESVKSEE"
     gene            16261..17202
                     /gene="fmt"
                     /locus_tag="PK898_07110"
     CDS             16261..17202
                     /gene="fmt"
                     /locus_tag="PK898_07110"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOU19839.1"
                     /translation="MSERAPRIVFMGSPEIAVPTLRGLAQFYPVVGVVTQPDRPAGRG
                     RLPKPPAVKLAAAAEGIPVIQPEKMKDPGVFETLQEWNPDLIVVMAFGKILRKNVLDL
                     PRYGCINIHASVLPRWRGASPIQAAILAGDSETGISIMQMDSGMDTGDVYAIERCPID
                     PQDTAATLSEKLANLGADLFLRSLPLILDGTLSAKPQSDEGVTYAPLISKEDGKLDFR
                     LPALQSERKIRAYHPWPTAYFFHNGTSIKVYKAHVVEISAMDTVPGKTFVYRGLPAVM
                     TGEDALVFDEIQSAGRKVLSGKDFLSGYRNWEEGLAE"
     gene            17199..18236
                     /locus_tag="PK898_07115"
     CDS             17199..18236
                     /locus_tag="PK898_07115"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOU19840.1"
                     /translation="MKIICFGAGAIGSYIGGSLQSAGHDVTFIERKESLPRIRSNGIQ
                     IEDVKGNIHKIESPHVTDDAIEAVKECQFDAAVIAVKSFDTERLMKSLIEVIGLLPPF
                     ISLQNGVENEALLASYLGKAKVVPASVCTAISRKQDGLIKVEKLRGIGITAGHFLSED
                     IYKAFRSANLKPALIADGTAMKWSKMISNLLANASSAILDMTPAEIFSDPDGFRLEIQ
                     QIREAVRVMDAYTIPVINIPGIPVKTLVWAVQNLPEKLLRPVLINAVGGGRGEKMPSF
                     YIDLHSGQSQNEVEYLNGAVVRFAKEKAIHVPVNSGYYRILTALVKKELPLETYRHQP
                     RKLAQDIFANE"
     gene            18510..18665
                     /locus_tag="PK898_07120"
     CDS             18510..18665
                     /locus_tag="PK898_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19841.1"
                     /translation="MEINKEFLFKDKKPNKPGKSDDTKKPAAAPKPAAPAKGPQKPGN
                     EPGKKGK"
     gene            18811..>19268
                     /locus_tag="PK898_07125"
     CDS             18811..>19268
                     /locus_tag="PK898_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU19842.1"
                     /translation="MSVLDVIRKRRSIRKFESRNIEQEKLDSIIEAGRLAPSASNQQS
                     WKFVLVTDKRNLIELAHACHDQMFVAEAPAAIVVCSEDNRMMSCGQSAATIDCSIALS
                     FMILEATDLGIGACWLGSFEPEQVKEVLDLPKTYTVVAVSPLGYPAEIPNP"
BASE COUNT         5313 a         3996 c         4420 g         5439 t
ORIGIN      
        1 gtcccatcta tcttcattga acattatctt caatatacga agtctgctcc aacctattct
       61 ttttccctgt aggggacggc gtccttgcga agcacaccga atttgtaggg gacggcgtcc
      121 tcgacgtccc ttctaccttc attcaacatt atcttcaata tacgaagtct actcaaatct
      181 gttctttttc cctgtagggg acggcgtcct cgacgtccca tctatcttca ttgaacatta
      241 tcttcaatat acgaagtctg ctccaacctt ttaaaatttc gccatagaaa agatgtctac
      301 ttatgaaaca caccgagatc cgagatgcta acgtctggtg aacactaagt tagtaatgtc
      361 atcttttatt gcagatccgt ctttctctat accgggaata attgtcatct tcctcaatgc
      421 aaatatcatg tttatgaaaa cttatattct tttacatttt tatagaatgt aaatcactat
      481 gccttaattt ggcattttaa aaattcatat tcatcatttt attaaccgag aaaatgtctt
      541 gttctattca ttttctcggt aatattcatc ttccaaccat tttataggat tgtcttcaat
      601 atactgccaa atattttcat aatctttttg atcacgaatc acatgatcat aataagaccg
      661 ctgccacatc gaaaaaccac attctttatt ggaaaatcgt ttaagagttg aaaccaaaga
      721 tggaattctt tcatttgcgt gttgtaaaat atttgattgt ttttcatcat tcgttatgaa
      781 aataatcata tgcatatgat tcggcataat tacatattta tcaataataa tatctttata
      841 actttcatta attgattgaa tgatcttctc aacaattaat ccatattgcg aaaataaatc
      901 attagaatac aaaagatttc cgttattgtg aaaaactctt ataagatttg gtttccagaa
      961 tatgttccga tgattatcgg tacatatcgt aataaaatat ccaccgtttc ggccataatt
     1021 gtattctttt agtcggtttg tttttctgat cggctggtcc atgatcttga ttataatgtt
     1081 catctttggg catataaaaa aatttggatt atttatctaa agaatttttc gatttcatcg
     1141 agatccttat tcgtaagaga ggatatattc gatcctcagg aatgataatg aaaggcgacg
     1201 atccttgatt gaagtcagtt attcccatcg aaagagaaaa gatgatgtat ttttggcatt
     1261 ttgaatcact gtaatccgat taggaattta cgatggaatt gaattcacaa aatgaagtaa
     1321 tcgtatgttt aggttcacag aacgtcagcg cctcgtacct cggcgtgctt cgcaaggacg
     1381 ccgtccgata cagaaatctc cgattccgtc gggagagggg aaaatagatg aaaataaatt
     1441 tgaaagatgc gaaatcgtct agatacggtt atgcgggacg tcgaggacgc cgtcccctac
     1501 agaagattgg ttttaataaa gcataatttt tgaatatacc aattgcggga cgtcgaggat
     1561 gcagtctgat acagaaatct ccgattccgt cgggataggg gaaaatagat gagaataaat
     1621 ttgaaagatg agaaatcgtc tagatacggt tatgcgggac gtcgaggacg ccgtccccta
     1681 cagaagattg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgaatat
     1801 accaattacg ggacgtcgag gacgccgtcc cctacagaag attggtttta ataaagcgtg
     1861 atttttgaat ataccaattg cgggacgtcg aggatgccgt ctcctacaga agattggttt
     1921 taataaagca taatttttga atataccaat tacagaacgg caaggacggc atcccctaca
     1981 aaaaccttcc ctcttcatgt tgggaggggc gtgtcaaaca aaattatgat atcggcggat
     2041 tattcgttat gctttcatcc tgttcggttt cttcttcttc ttcggcactc atctcccaaa
     2101 ctttattggc acgatcaacg tagcaaatgc cgtccagatg atcgatttca tgttgaaaga
     2161 ttcgggcaag ccatccgttg gctgtaatat taaacggttt gccatgaatg tttaatgctc
     2221 gaattttcac ggattcggcc cgttccactt cgcctgccaa tcctctgaaa gaaagacatc
     2281 cctcaacgcc tatcgtcttt tcttccgaca tccaggtaat ttccggattg atcaatttgt
     2341 aagagatcaa aggcgaatct tctatttcat cattttccgg atattccacc acaatcagac
     2401 ggatcgattc accgatttga ggtgcggcta atccgacgcc gttattttcc cgcatggttt
     2461 ccaacatatc cgaagctaat tttcggatgg atgagtcaat tcgtgttatt ttatgggctt
     2521 tccgcctcaa aatcggatcg ccggtctgtt ttacttccaa aatcatattt tccttttggt
     2581 atcagaggct cgaagttctc gagtgaactt ctgccgaaaa ctttttaata ttaacgaata
     2641 gttattctaa ccgatttttt gcagccttcc gattaaaata atgaatcggt gaatctgtta
     2701 catcattttg aataaccggt tgcttttccg gatcattcaa ttgctttttt gcatcatttg
     2761 cttccatttg tttaatgatt tccaactgta ctttatcggg gtggggaaca taaacaaata
     2821 gaagctctga ttcacctgat aatatcttta tggttccgta atcaaaaaga tattgaaaaa
     2881 tcccgctctt tttatattga acgctttgga ttttgctgat caaagtcatg gttacttctt
     2941 ttttcgcgaa tggtttccga ttgatgtctg caacccgttc ggcaccgaca taatacagat
     3001 cgttcgacca atcaatcaag gcgtaaataa tccaaaaaat aaaaacgaca acgagaatta
     3061 ataacgctgc ttttatctca aaaacttcaa atatttccca atcccgagtg attccgacaa
     3121 cgattgtgac aagtgaacat aataataaaa ggatggggat aatcgcctgc cggacaaaat
     3181 atgcccaatg tgcccgataa accattccct ctttttcagc cgcattgccc ttcttttgct
     3241 ttacatagga ataagcggtg ggggtatatt cactgatata gtagaccgcc ggtttcggcg
     3301 gacgatttcg tcgatcggct ttgaccggcg gtttgattct caaatcggca aaaacataga
     3361 tgcaatagaa caaaaaaatg acgacggaaa taccggcgat aatcggaatt atcttgtaac
     3421 ggccaaacaa aaaccagcaa attaaaagac aaaccgcgag aatccccaat cctttcagca
     3481 agcgtaggat gtggcttgcc ggtaaaccgg tgttttcctt tgattctcgc ggttcaagtg
     3541 acatttatca gatttgcttc aagaactcta attcttcggc atccgctttc ttcttgattt
     3601 gaattttatc gcctcgtttc aattctccgc ccaaatactt ggttgagatc ggactttcca
     3661 cgtatttctg aagtgcccgc ttcagcggac gtgcgccaaa tgcctggtca tacccgatgg
     3721 tcgccagcca ctgccgggca tcatcgtcca gctcaaattc aatcccgttt tcactgaggc
     3781 gctgacgaac ttcttccatc tggatatcga cgatcttttt catgtcatca agtgataatt
     3841 tggagaacat gatgatttca tcgatccggt ttaaaaattc cggtctgaag gttgatttca
     3901 gcgccttatc cactttcagt ttggcttccc gatcttcggc ttcatctttt gatccgatga
     3961 aacccaaggt tccgccgcgg ttaatatatt cggtacctaa gttgctggtc ataatcaaca
     4021 cggtatttcg gaaatcgact actcttcctt gtccgtcggt caaacgcccg tcatccaaaa
     4081 tttgcaacag ggtgttccaa acttcggggt gtgccttttc aatttcatcg aaaaggatca
     4141 cgcgataagg gcgacggcga accgcttcgg tcaattgacc gccctcttcg tatcccacat
     4201 atcccggagg agcgccgaac aacctcgaaa cggtatgctg ctcgcgatac tcactcatgt
     4261 cgatccggac caatgcatcc tcatcatcaa acagatattc cgctaatgct ttggcaagtt
     4321 cggtttttcc gacgccggac ggaccgataa agataaagga accgatcggc cgtttgggat
     4381 ctttcagccc tgatcgcgcc cgacgaatcg catccgcgat ggcatgaatc gcctcatcct
     4441 gaccgatgat cttctcatgc aagcgatcct ccatatgaag gagcttctcg ctttcactct
     4501 caagcatctg atttaccggt attccggtcc attgagcgat aacttgggcg atatcatcca
     4561 catcaacaac ttcatccagt ttatgatccg attcccaagt tgcttttttc tggttgaatt
     4621 catcttcgac acgcaagcgc tcggctttaa tttttgccgc tttttcataa tctcgttcca
     4681 aaccggcatc ttcttcctct tttgccatcc gatcaatttc gttcttcatc tctttcagat
     4741 cgggaggaag gctgtataac gcaaccctta actttgcgcc ggcttcatcg atcagatcga
     4801 ttgctttatc ggggaattta cgatccgtga cataacggga agataagcgg acagccgctt
     4861 gtaatgcatc atccttataa atcactttat gatgttgctc atatttgtct ttcaaaccgt
     4921 gcagcatctc gatcgtactg ttttccgaag gttcttcgac ataaatggga gcgaaacgtc
     4981 gttccaatgc cgtatctttt tcaatatatt ttcgatattc atcgagtgtg gtcgcaccga
     5041 tacattgaag ttctccgcgg gaaagcgccg gtttcaacat attcgaagca tcgacagccc
     5101 cttgtgaggc acctgcgcca actactgtgt gcaattcatc aataaacagg ataacttccc
     5161 cgtttgattt tttaacttca tcaataatcg cttttaatcg ctcttcgaat tcgccccgaa
     5221 actttgatcc ggcaaccaac gctcccatgt ccagcgaaat cacttttttc ccggataata
     5281 attccgggac atcatttgcg acgatttttt gagctaaacc ctcgacgatc gccgttttcc
     5341 cgacaccggc ttcgccgatc aggacgggat tgtttttagt acggcggctg agaatctgca
     5401 tcagccgtaa gatttccaca tttcttccga ttaccggatc caattttcct tctcgagcct
     5461 gatcggtcag atcacgggaa tatttttgca gatttttata tctggaatct ttgttttgta
     5521 ccggagcatt tttgttatcg tgcattttta caacaatttc atagactttg gcccgtgtaa
     5581 tgccgaattc ttccattaat tttgcaacgg gtgtcccttt ttcatttaga atcgctaaaa
     5641 aaatatgttc ggtagaggta tattcgtctt tcagacgatt ggcttcgata tacgcctggt
     5701 caacaattct tcgaacattg ggcgtaatga atatttgtcc ggatcctccg ccgtaaatgt
     5761 tggtctttgt actggttcga agcatgtaat ctaatctgtc tgacagaaca gcggtggaaa
     5821 cattcatctc atctaaaatt tgagaaacgg tcccctgcgg ctgttcgatc agcgccaata
     5881 aaaagtgctc ggtatcaatt tgattttgtc cgtaacgttg aagaatctcg acagcgcgtt
     5941 gggctgcgtc ttgagcactt tctgtaaatc ggtcaaatcg catcataatt tctttccatc
     6001 tttctcgtta aatcgaaccg aaaaatcagt tcgaatcacc gttttaatcg ttcatttatt
     6061 gcctaaaaag gtcaacgtga ggttattgta gcatttcact gtaagaacat tattagattt
     6121 acgcaagagt caggtctgag cagacgcatg gaattttatt taccgagtac atcccaaggg
     6181 gtgatcaaac ccagcagttt ctcttgagag ttgcccgttt cggtcaggaa aacaattgcc
     6241 aatcttttct gccgtttttt atagttggtg ttaaaaagtt caaggatgtc cgtatacaag
     6301 gcatctttcg gcgcaaattc aaaatactca ttcgagtgat tttcaatgtc caacaaattt
     6361 ccgaattctc taattcgcat tcctctttga agcgcaggca tattgtttcg aatatggaaa
     6421 ctgaaaacgg tgcttttact gaaaacgccg agcattttct cgccgtcgac aaccggaacg
     6481 tgtgaaaacc ctcttaattc catcatacgc atcaccggat aggcttcttc gtctaaacgg
     6541 gttttttgaa ttttatccgc ccaagttgca tagttcatcg cttttttggg ctgttccact
     6601 ttttccaaga tttcttctaa aaccgcgccg atttcatccg acggctcaac cggatatttg
     6661 ccgtttacca gcggagaatg gacgagcaaa tttcgaatgt ctcgaatcgt cgacaattct
     6721 tcccggtatg gtttcccttc acttgaattc gaaaagcgga tgatggggct ttcatacctg
     6781 tcgttataat aacttccgcg acctttcaat tttgtttcca gattgttgta gagatcaaga
     6841 aaaacttcgg gatccatgca ttttgtcctt tctcttccgt tattcggtta ttcggctttt
     6901 atttgattat atctttatgg ctttctgttt tcggataaat ttaagatatt atcggactgt
     6961 ttattttaaa tacaccgaat tgaaattata atttagctgt ttcttttagt ttgtcgaata
     7021 tcaagattgg aagagccttt ttttatgtcc aaaaaacaaa aaaataataa aaacagcaag
     7081 cagaatgcgg gcgatctatt cggattggct ttgtggattt ttgcggcatt cggattggaa
     7141 attgttttaa tctttatcga aagcaaaatt tatgcggttt cttctttgaa ttggacgata
     7201 aggcaaaatt gtcttcattg ggcattgatc agcctttttt gggcgattat ctctttttta
     7261 atcgtacgca gtgccaagaa agattatgac tttgatatat tcaaaagaca gcaaaacccg
     7321 actatgactc aaattctatt ttctgtgctt ttggtaattg tcggaattct tttcgtctct
     7381 tatatgagcg gaggattcaa accggtatcc gaatataaaa aactgggatc ggtacagttt
     7441 atatttcaat atattaccta tttcttcaaa tcaatcttgg tcatgttggc aatcgctttg
     7501 ggacaaaaag ccggagatct ttattttcaa aaagaccaaa ttccatgggg cggttatttt
     7561 ttggcggttt cttggggatt cattcaattt attattcaaa atcccgcaac cgggctgttc
     7621 actgtggcat tatctgtctt atttggggtt ctgttcatga ttaatcataa aaacgcgcga
     7681 attgtttttc cgatgattgc cattgttctg atgctctaaa agagtgatca ttaataaaaa
     7741 gaaagggttg aagagatctt ccgatgggaa aaatgatttt aattggtgtc gtaatggtga
     7801 tattatgtat tgttgccggg aaaattataa aaagaacgat gtaaattgac agcgtttttg
     7861 tttccgattt taataagctc acattcacat cacaatgcca atcaaatatt gtgagaatat
     7921 tcattaataa ccgcaaaaag catttacaag ataaaaagga aaaagtataa tttgaaaata
     7981 gccgtagcca agaggtatgg ctttttattt ttgtctgttc aatctataaa atctcttagt
     8041 gaaaggggat tcatatgaaa aaatcagctc tgcttgcaat tgtagttttg tgtctggctg
     8101 tttcaacagg cgtgcttggc ttcatgtatt caaccaaagg agataaaact gcggaagaag
     8161 cggctccgat ggtacaaatc ggggtgatga cgaataagtg ggcagcttcc gctcacggag
     8221 attcatcttc catagcattc agacattggg atgaagaagg tgaagtaccc gttacctgtg
     8281 ctaaatgcca ttcgtctacg ggaatgcagg attatctcgg agccgatgga acagccgcca
     8341 tgacggtgga taaacctgcc aaagtgggat cggttgtaga ttgttctgca tgtcacaatg
     8401 aagtaatcca gaacacaact tcggtggtct ttccttccgg gaaagaagtc gcagccgaac
     8461 cgtcttatgc ctcttgctgg acgtgccatc agggcatgga agccggaatt aatggatcgc
     8521 tggatcagaa aatcggttcg accgctccgg atgataaggg cgaaaatctg ggcttcgtca
     8581 atccgcatta tttgaatgtg gcgggtattt atctgggatc cgaagcaaat gccggttatc
     8641 agtatgaagg aaagacatat gcggctcgct ttgagcacgc ttcgggtgta cagacctgta
     8701 ccgaatgcca tgatcctcac agcctgcatt tggcagatcc gggattggaa aaatgctcaa
     8761 cctgccatgc ggaagtcgaa acctggcctg atcagcaaaa aattcgccgt accaaagaag
     8821 acctggacgg tgacggaaat accgacgaat ccacctatga ggaaatcaac ggattactcg
     8881 agcgggagct tcaagcgatg gtgacctatt cggttgatgt tgccaaagtt cagatcggta
     8941 attccgatgt ttatccctac tggtttgcag ataaaaacgg aaacgggact caggaagagg
     9001 atgagaaaca gtatgccgac tggacaccgc gtttgatgta tgccgcatat aactttaaac
     9061 tggtcaagac ctcagccgga tatatccata atccgactta ttccgcacaa ttgttaatcg
     9121 attcgatcag tgatttggct gcgggtgatt cggcaatttc ggttgacggt atcgagagac
     9181 cgtaggaaga aaggtaatca aacatgaata ctgctgccag tcgttcggca agaagagctt
     9241 atatcgctca actgcgagtt aaaaggatat tctggatttg cctgtttgtt gtcattgcct
     9301 tgttcccgtt ggttgtcgct gcaatcaata acacgacaaa cgggacgtgg cgggcggtgc
     9361 cgcgtgagat cggttctttt gccgggatgt tagggttgtc cctgatgatc ctttccttta
     9421 ttccgattat gcgagaaaag aaaattacca gcgcttttaa tctggatgtg gtatacaagt
     9481 ttcatcattc atgctccatc cttggttttt ggctcgtggt ttttcatctt gtagctttgt
     9541 ggatcaacaa tcaggaaatc ggacgactgg taaatatatt tgcagattat ccgctttata
     9601 tgaaagccgg ccccattgct ttattggccg ccggctgttt ggtctatttt tcttatttcc
     9661 gtaaggaatt gaagcttaac tatgattatt ggaaaatcgg gcattccctt gttgcgatac
     9721 ttatggtggt ttttggctta attcacactt ttggggtgaa ttattacagc ggcaatgcac
     9781 taatacacgg ttactatgtt ttcttgaccg tcttggctgc gattgctatt ctgtggctgc
     9841 gcgttttctc ttcactgtct gccatgtgca aaccttatgt cattagggaa gtgctgattc
     9901 gcaacaacgc aaccaccgaa ttaagactgg aattcgccgg aaaaggaaat caaaaaccga
     9961 ttcgttatca tgccggacaa atcgcatgga taacggtgcg acattcccgc ttttctttca
    10021 aagaacatcc gttctcgatc gcttcctcgg atatcgacca aaataaaatc ggctttgcca
    10081 tccgcgagtt aggcgatttt accgctacga ttaaggatct gaaacccggt gagatcgttt
    10141 atgtcgaggg cggttttggc atctttaatc catatatatt gggcgatgaa ggttttgtgt
    10201 tgattgccgg cggaatcggt attgcaccgg ttatgggaat tctgcggtcg atggcagatc
    10261 gaaaagatcg gcggcatgtg gtcctttatt atgggagtcg tgacaaagaa agcatcggat
    10321 tttatgacga actgaatgaa ctgtccaaaa aattaaattt ggaagttgtc catgtcctcg
    10381 agaagaccga tgatccgtcc tttgaaaaag gttatgtgac caaagatgtg atcgaacgac
    10441 attacccgaa aaataaggat cgatacgacg tgtttatctg cggacccggc ccgatggttc
    10501 agattgtcct tccggcctta aagaagatcg gaattccgaa agagaatatt tggcccgaat
    10561 tctatgatat ggcttaactg gaagttttgg aaatgattaa aaaggctgcc cgtcgatttc
    10621 ttcgggcagt ctttttatta cgaaagtata atacctaaat aaggtatttt ttgccgataa
    10681 aacgaacgaa ccttcaggaa agaaaatgga tcaatacgat attattatca tcgggggagg
    10741 cgtggtcggg tgtatgatcg cccgcgaact ttcacgctat tccccgaaag tgcttttaat
    10801 tgaaaaaacc tccgatgtcg gctgcgggac ctccgcagcc aattccgcgt taattcatgc
    10861 cggatatgac ccgttaccgg gaacgataaa agcggcattg aatgttgcgg gaaatcgcat
    10921 gtgggattcc ctggcttcgg agcttgattt tgattttacc cgttgcgggg attatgttgt
    10981 cgcgataggg gatgaggaac tgcccgcatt ggaccgattg aaatcacagg gggtccaaaa
    11041 cggagtctca ggattgaaaa ctttttcagc ggaagaagtc cgaaaaagaa ttccgcggat
    11101 taatccggag gtaagcggtg ccctgttcgc tgaaagcgcc ggaatttgcg atacttttgc
    11161 cgtaacgatt gcagctgcgg agaatgcact tcataacggg gtggagattc gcctgaatac
    11221 cgaattcaag ggctttatta ttgaaaaaaa tgtgattcgg ggaattcata ccgatcgcgg
    11281 agatttcggt tgtcagtggg taatcaatgc tgccggatta tatgccgatg aagttatgca
    11341 tcaggcgaat tgtcgccttg attttaaaat aaccccacgc agaggggaat attgtatttt
    11401 ggatccggat ttattccgaa tcgatacggt aatatttccg gtaccgagcg aaaaaggaaa
    11461 gggtgtgctg gtttttaata ccactcacgg gaatacggtt gtcggtccca cctccgaatt
    11521 tgtttccgat aaagaagaca aatcgatcag tccggaagga ttgaatgatc tgctgaaaaa
    11581 tgtgcggaag ttaatcccgt ccgttgatct gcgatggacg atcgcgacat ttgccggatt
    11641 gcgagcaacc gggaatgccc cttgtccgga ccccgcgatt cattatgact ctgattttat
    11701 cgttgaatcc gcacaggaga ttcatggcct gatcaactgc gccggtatcg aatcaccggg
    11761 gttaaccgcc tctccggcga ttgcccttcg agttacggaa cttctgaaag aggagggcgt
    11821 ccgattaacg ccaaacccaa aatgggaccc gattcggaaa cgccggccgt cacccaagca
    11881 tttatccgaa tcggaacgcc aaaaattaat cgaagcggat aaccgttacg gaagaataat
    11941 ctgccgttgc gaggggatta ccgaggggga aatcgttgcg gaaatacatg cccctatccc
    12001 cgcccgaact tatgatgcca tcaaaagacg gacttggacg ggaaccggcc gctgtcaggg
    12061 cggatttgat atgagccgag tggtgaacat tctggcacgc gaattaaaag tctcaccatt
    12121 ggaagtccgg aagaatgatc cgggatccga atttcttttt cgggaaagta aggcgatcgg
    12181 aggaacgtta tgaaccttcg atcggaatat gatgtgatcg ttatcggtgc cggaccggcc
    12241 gggttggcgg cagccatcgc cgcccgacaa aacggatgcg atcaggtttt attgatcgat
    12301 cgcgatgtgg agccgggggg aattttgcag caatgcgttc acaacggttt cggtttggaa
    12361 acattcaaag aagacttacc ggggccttcc tacgcgcagc gatttattaa tcgtgcaatc
    12421 gattgcggag ttcaattcct gatggatacc atggttttgg ctgtatcccg ggacaaacag
    12481 gtaacggtct ccggcgcttt ggtcggaatt cggacgctgc aagctaaagc cgtcatcttc
    12541 agtatgggat gccgggaacg cagccgggct cagattcgaa ttccaggcac acgtccggcc
    12601 ggagtgtata cagcagggac tgcacaacgc tgggtaaata tcgacgggtt gatgccggga
    12661 aatcgtttta ttatcttggg atccggtgat atcggaatga ttatggctcg acggctgaca
    12721 ttggaaggtg ctcaggtgga acgtgtggtc gagattatgc cttatctttc cggattatcg
    12781 cgaaacttgg ttcaatgcct gcaggatttt gaaatcccgt tgtttttaaa tcaaaccgtc
    12841 agcagaatta tcggaaaaaa acgggttgag gcggtggaaa tttccgcggt ggatgaaaaa
    12901 cggcttccca ttccttcttc ggctgaaatt attccctgcg atacgttgtt attgtccgtc
    12961 ggattgatcc cggaaaatga gttgacccga agggcggggg ttgaaatgga tgagcggatc
    13021 gggggagcgg tggtggatga tactttttcc accggcatcc ccggaatgtt tgtcgcggga
    13081 aatgtagtcc atgtttatga tttggtggat tatgtcagtc aagccggcga aaaagccggg
    13141 tcatcggcag ccgattatat tcgcggaaaa acatcctatt cggagaaaaa ggtaaaaaca
    13201 acggccggaa aaaacgtcag atatgtggta ccgcaattaa tccgtcctga atcgctgaaa
    13261 gagacttcga tattgttgca gatgcgggtt gtcgctccac ttgaacatag ggtggaactg
    13321 attgtccggg atggtgaaaa agagattacc cgaaaacgac tcccttatgc cagacctggg
    13381 gaaatggttt ctgtttcact tccgcaatcg gcttatgata ttgttcaaca ggcggatgaa
    13441 ataaccgttg aaattctttg aaggggaaaa atgatcgaaa aaagagaatt aatttgtgtg
    13501 acctgtcctc gcggttgccg cataacggtt gagatggaag atgaaaaaat aatccgcatt
    13561 ttgggaaata cctgtgaacg cgggaaaaaa tatgccgaat cggaaataaa agatccgaga
    13621 cggatgtttg cttctaccgt ccgaattcgt aacggagttc atccggtact gccggttacc
    13681 gttacacagc ctttcccgaa agatcgattg aaagacctgt cggctgaatt acgaaaaaca
    13741 gaagtgaccg cccctgttcg gatgggtgat gtgattgtaa agaatgttct gggtacgggt
    13801 attgatgtca ttgcatcgcg tgatatggaa tctgtccgat cgtagcgatc atggcataaa
    13861 acttgcactt aaattgttga tagaattcaa attctgcgga cgatttttag gaatgaatca
    13921 ggagtcaaag gatatttatg gaagataaaa ctgcggagaa aaaaatctac tcatccggtt
    13981 atgcgggaac gattcccggc gttcataaag aaatcaatga agatttcgtt atattctttg
    14041 aaaaggaacc cgaaggaacg gtgggcgcct cgaatctgta tgtcctgacg gatggtgtga
    14101 cgggaacgtt tcatccggaa atcacagccc gcttcgcagg gaagaaaatc ctttatgaat
    14161 atttcaaaag cgccgacttt atcgatgcga ataaattggc gttagccatg cggaatgcca
    14221 ataacgaaat ctatgcttat gcggaattaa aacaggaaaa gatggcaacg gttgcgcttg
    14281 ccactgcaat aacggatggg aatgccgtta tcggcacgat cggcgacagc cgtgtttatg
    14341 tcgtgcggaa cgataaagtc tatcaaatca ccgaagatca gaatattctc gaagaaaaag
    14401 ttcgaaaggg tgaattaacc aaagaagaag cgtttgaaac cgaagctcac aaacctgtta
    14461 tacgggcatt gggaagtgaa aaagatattg tcgttgatat ttatgacggt atcgaagtaa
    14521 aaccggggga tgtcttgttg atgtgcagtg acggactgac cgcttatatc ggtaaggatg
    14581 agattttaga agctgttcaa gccgacaccc cgaaagaaat cgttcgaaat ttattggctt
    14641 atgccgccaa tcagaatagt ccggcggatg cttctgcgat tgcaattaag atatatgacg
    14701 aaaacaccat tcagacggtc gtgaggcagc cgggcacctt gccggtcgat accgacttga
    14761 ataaagagac caaagaactt gatttaatga tgaaatcaaa accgaggcat caacccgaaa
    14821 aaacggagaa aaaaggtaca aaaaagactt ccgcttatat catttcagct ttactgatct
    14881 gtttggtgat cgggggaata ttctggtttt tctctgacag cgaaggtcgt ccgtcccttt
    14941 tcggcccgag aatgactgcc accgctaccg ttgatccggt tcaagcgact ttggcaatca
    15001 ttcatcagac gcaacaagcc gaagcgattg cgcggctttc tgcgacacca accgctacgt
    15061 tgacgccgat gccgacaatg acatctaccg ttaccgttgt tccgacgttg tttgatctga
    15121 atgtacctgt agccggagcg gtgaccgaag ctgccgaatt cggcggaaca cagacggtat
    15181 tgactccggg aacagcggaa gttcaagtga caattacacc gacgccggtt gacaaaggac
    15241 cgatcgtttc cgaaaaagat caggctgaaa tggtatatgt aaaagccgga gaatttctgt
    15301 tgggatccga tcccctgaaa gacgggtatg ccaatacgat cgaagaaact ccgcagatta
    15361 ccgtttattt ggatggtttt tggatcgata aatacgaagt gacgaatgcg agatatctaa
    15421 agtgtgtcca agccggggga tgtaatcaat cttcttatat gctgttgaac gatccgacgt
    15481 atgcggatat gccggtgact tatgtaaccg tcgaggaagc cgaacagttt tgcgaatggg
    15541 ccgggaaaag attgccgacc gaaatcgaat gggaaaaagc agccagagga accgacggca
    15601 ggatttatcc ttggggtgac gaagagccgg tcgagggaac acgttatgcg aattatccgg
    15661 gttataccga tcccgatgca caacaggtaa gcggactgtt caaagtgggt tctttcccgg
    15721 aaggagcaag tccttacggc gccatggata tggccggaaa cgtatgggaa tggacttcat
    15781 caaagtatca agcggactat tatcaaacat tggtcgatga agcggaaatg ggtacggaag
    15841 tgattcgtaa tccgaaggga tcacaaagcg ggatcgcaaa tgtgatccgc ggcggatcgg
    15901 ctgcggaaac ggaaatcaat aactatttgg cttatctgag aaccgctaac cggagttacg
    15961 taaatatgaa cagcagctat tatatcggtt ttcgttgtgt gttgccggat tcggaatcga
    16021 ccggcgaaac cggtgctgtc gtgcaggaaa gtgttaagtc cgaagaataa acggaacttt
    16081 ttttcatttc tcctcaattg aaagaagaac gtcaaacaac cattacccgg aacccagacg
    16141 ggtaatggtt gtttaaatga tattggatcg gaatgatatt attcacaaat ccaagcaaac
    16201 aaaccgaaaa agccctataa tcggtgtgag aaaaaaagaa aaacgggaag gaagattttt
    16261 atgagcgaac gagcaccccg aatcgttttt atgggatctc cggaaatcgc cgttccgact
    16321 ttacggggac ttgcgcaatt ttatcccgtt gtcggggttg tcacccaacc ggatcggccg
    16381 gccggcagag gtcgattgcc gaaaccgccc gcggtaaaac ttgcggcagc tgccgaaggg
    16441 attccggtca ttcaaccgga aaaaatgaaa gatccgggtg ttttcgagac ccttcaggaa
    16501 tggaatccgg atttgattgt tgtaatggct ttcggaaaaa ttctccgtaa aaatgtattg
    16561 gatttaccgc ggtacggatg tataaacatt cacgcatcgg tattgccgcg ttggcgcgga
    16621 gcgtctccga ttcaagccgc gattctggcg ggagattcgg aaaccggtat ttcgatcatg
    16681 cagatggaca gcgggatgga taccggtgac gtatatgcaa tcgaacgatg tcctatcgat
    16741 ccgcaagata ccgccgctac cctgtcggaa aaattggcaa atttgggagc cgacttattc
    16801 cttcgatctt tgccattgat tttggatgga acattatcgg caaaaccaca atcggatgaa
    16861 ggcgtcactt atgctccgtt gatatccaaa gaggacggaa aacttgattt ccgtctgcct
    16921 gctcttcaat cggaaagaaa aattcgtgcc tatcatccgt ggccgacagc atattttttt
    16981 cataacggaa caagtattaa agtttacaaa gcccatgtcg ttgaaatttc agcgatggat
    17041 acggttccgg gaaagacttt tgtttatcgg ggtttaccgg ctgtaatgac cggtgaggat
    17101 gcgttggtgt tcgatgagat tcagagcgcc ggaaggaaag tgctttcggg aaaggacttt
    17161 ctttcgggat atcgaaattg ggaagaagga ctcgcagaat gaaaattatt tgttttggtg
    17221 cgggagcgat cggctcttat atcgggggaa gtcttcaatc ggcaggtcat gatgttacct
    17281 ttatcgaacg aaaagaaagt ttgccgcgaa ttcgatcaaa cggaattcaa atcgaggacg
    17341 taaaaggaaa cattcataag attgaatcgc ctcatgtcac cgatgatgcc atcgaagccg
    17401 taaaagagtg tcaatttgat gccgctgtta ttgcggtgaa atcttttgac acggaaaggt
    17461 tgatgaaatc cctgatcgaa gtaatcggct tgctacctcc ttttatcagc cttcagaacg
    17521 gtgttgaaaa cgaagctctt cttgcttcct atttgggaaa agcaaaggtt gtaccggcat
    17581 ccgtttgtac cgccatcagc agaaaacaag acggtttgat caaagttgag aaactgcgtg
    17641 gaataggaat aaccgccgga cattttcttt cagaggacat ttataaggct tttcgatcgg
    17701 caaatttgaa accggctttg atagctgacg ggactgcgat gaaatggtcg aaaatgattt
    17761 caaacttgct ggctaatgct tcctccgcaa ttttggatat gacacctgcc gaaatatttt
    17821 cggacccgga cggattccgg ttggaaatcc aacagattcg ggaagcggtc cgggtaatgg
    17881 atgcttacac tattccggtg atcaatattc cggggatacc ggtaaaaact ctggtatggg
    17941 ctgttcaaaa tctacccgag aaattattac gtcccgttct aataaacgct gtcggaggag
    18001 ggcgtggaga gaaaatgccg tctttttata tcgatttgca ttccggacaa tctcaaaacg
    18061 aagtcgaata ccttaacgga gctgtcgtga gattcgcaaa ggaaaaggcg atccatgtgc
    18121 cggtcaacag cggctattac cgtattttga ccgcattggt aaaaaaagaa cttcctttag
    18181 agacatatcg gcatcagcct cgtaaacttg cccaagatat atttgcaaat gaataatttg
    18241 aaccgcttac ataaaatttc cattaaattc aaggcaaaag gattaaataa atcgtattaa
    18301 tttctatttc aactattgac agaagtgcta tactcatttt cagttaatta gagaaggagg
    18361 atttttcgcg gaaaatacat acgggatcat tccaaaaaag caatcaagtc gcctcggacg
    18421 gcgcagtaat tccggaagga atcgttacag cgggtcgaaa ttcatgggaa acctttccta
    18481 aaaatttgaa tgaatgaatc gaggatgata tggaaatcaa taaagaattt ttattcaagg
    18541 ataaaaaacc gaacaaaccc ggaaaatcag atgatacgaa aaaaccggct gctgcaccaa
    18601 agccggctgc acctgcgaaa ggtccccaaa aaccggggaa tgagccgggc aaaaaaggga
    18661 aataaacccg aacaaaagtt tatctgaatt ttaagatatg ggagaagcct cccatatctt
    18721 tatttaattt cacttgatcg aaccctgata agtctgtaga ataattatgt aaagcgaatt
    18781 attgaactta tcagaaaagg gagggatcct atgagcgtat tggatgtcat cagaaaacgt
    18841 agaagcatca ggaaattcga aagtcgaaat atcgaacaag agaaactgga ttctattatc
    18901 gaagccgggc gattggcacc ttccgcaagt aatcagcaaa gttggaaatt tgttttggtc
    18961 acagataaaa ggaatttaat cgaactggct cacgcttgcc atgaccaaat gtttgtggcg
    19021 gaagctcccg cggcgattgt tgtttgttcg gaagataatc gaatgatgag ctgcggtcaa
    19081 tccgctgcaa caatcgattg ctctattgcg ctgtctttta tgattttgga agcgaccgat
    19141 ttggggatag gagcttgttg gttgggttct tttgaaccgg agcaggtgaa agaagtgctt
    19201 gatttaccga agacatatac ggttgtggca gtttcgccgc ttggctatcc cgctgaaata
    19261 ccgaatcc
//
LOCUS       DAOSMM010000043        19204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_2864_length_19204_cov_18.622591, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000043 DAOSMM010000000
VERSION     DAOSMM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 19204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19204
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2864_length_19204_cov_18.622591"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK898_07130"
     CDS             <1..183
                     /locus_tag="PK898_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19843.1"
                     /translation="ASPKIIGKINKNKIRFSVIHRFGLCEYVMSSQILLSSASMPKMS
                     RIIRIVIIAVDFISRR"
     gene            complement(832..921)
                     /locus_tag="PK898_07135"
     tRNA            complement(832..921)
                     /locus_tag="PK898_07135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(883..885),aa:Ser,seq:gga)
     gene            1030..2043
                     /locus_tag="PK898_07140"
     CDS             1030..2043
                     /locus_tag="PK898_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19844.1"
                     /translation="MYIQNSQIPQPILSVSQKTLDFGLVSAGEPAEGHGIAKLTITND
                     GDRILVGRIAIQVAWVTVDPPDFRLNPGESSEHIFTVRQNVQFSWTTRRMGSDFIALI
                     NSNGGSETIGGYYFLDSTKTEKKKKPIHPWLYALIPIFILLTAGIILFVSRQNEAALA
                     QQKTENSDSLYTRIAETYFAEMTENPTIISTEPPPQNTPNPLVVPNFDITPSPAPSMT
                     FTPWVAADYPNVEEFIRTYYKTLNERDYETAWWMLSEKMQQACCYTGEANPMTVYQNY
                     WDKVESVEVNYAFLQSYDTNPAIVNVGLTYHYKDGTTEETVNQFSIITDDVKKSLVID
                     EIR"
     gene            2119..2346
                     /locus_tag="PK898_07145"
     CDS             2119..2346
                     /locus_tag="PK898_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU19845.1"
                     /translation="MEITLDNVKKVITDVLKIDGSDITPETRFIEDLKADSMDQFFLI
                     DGFSEAFNVTISDEQAKEIKTVGDVIRVLSK"
     gene            complement(2409..3275)
                     /gene="sucD"
                     /locus_tag="PK898_07150"
     CDS             complement(2409..3275)
                     /gene="sucD"
                     /locus_tag="PK898_07150"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOU19846.1"
                     /translation="MGILINSSTKVIVQGITGREGSFHTEQMAAYGTQIVAGVSPQKG
                     GSWVLDGKIPVFDTMQAAMEVTEANASIIFVPARNATDALFEAADSGVELVVCITEGV
                     PVQDVVKVCQYMSNRGVRLVGPNCSGIINPGEAKLGVAPSDCVIPGKVGVISRSGTLS
                     YLVMSEMKSRGIGVSTCIGIGGDIVQGTTFVEALELFETDPYTERILLIGEVGGLEEE
                     RAASYVAYKMTKPVTALLVGKTAPQGKKMGHSGAFIEAGIGGIDSKIEAFESVGVRVV
                     TDIKEVPDALQG"
     gene            complement(3277..4416)
                     /gene="sucC"
                     /locus_tag="PK898_07155"
     CDS             complement(3277..4416)
                     /gene="sucC"
                     /locus_tag="PK898_07155"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOU19847.1"
                     /translation="MKLYEYQSKGFFKKYGIPIPAGHVTSIAREAKQIAEEIGLPVVV
                     KAQVLAEGRGMAGGIRLAKTPEETEIFASEILSAKIHGLPVQKVLIDEALSIEKEYFI
                     LISIDRAKKRPLLMMYDSGTIYIEAAANALFENLDRVYIDPLIGLQRYQIHQLAYKLD
                     FPGKYIRNLTEICMGLWRLFCDLDARMAAINPLVITTDQRILALDGKITLDENARFRQ
                     ESLTDFVDLGYLRYGELEAGKHGLDYIKMDGNIACMINGAGLTMTTLDLIEANGGKPA
                     NFLDLGAGVNTEKILAAMDILQNDPAVKVILVCIYAGMTHCDDVARGLLEAMNRYDSK
                     VPIVVYMGGSMSALGNQILSGSPAILVPSYEEAIRKAVETAAGQG"
     gene            complement(4435..5493)
                     /locus_tag="PK898_07160"
     CDS             complement(4435..5493)
                     /locus_tag="PK898_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU19848.1"
                     /translation="MARIFDVVEYPSEMTDEIVHRFPETGVADLRFGSQVIVRESQRA
                     IFYRDGKALDQLGPGRHTISTANIPLLTALLGVAFNHRTPFTAEIYFVSMREFADVKW
                     GTSQPIIVRNPGVGLGISLLQGYGTYSFQVSDPQQFVTQIVGASQSYSLSAIEDRLRA
                     MLLSKLQDTLGVITGEQNADVMKLIGLVEEIGAAVRAKAQDDFAAIGLALKSFYIASL
                     KPSEKSAEELRAMGMLDMDVYTQLQAADALREAAKNPSGGAGLTAGIGAGIGLGDIIA
                     NTYRNQPPAQSNASAAADQPATAESGEVLTPAEAAKLLKVSEEDVIASIESGELKARK
                     IGSAYRISRESLNEFLKG"
     gene            complement(5497..6048)
                     /locus_tag="PK898_07165"
     CDS             complement(5497..6048)
                     /locus_tag="PK898_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19849.1"
                     /translation="MENLNDLVKSGKNSFQELMEKIPGIKGYKAKEDRRDTDKIIREE
                     IAKKFEEEWSRISAIQRDLIKAGNLNAVSDLESAAIKIRQFTDRIKTAPYGYAGLLDA
                     IKVDDAALTRLYDYDLYLLKMADTVKASVDEVEKAVASDPDNIKNAISQLTKAAQDSL
                     TAFNQRTEVIIGASDLNSGKSGE"
     gene            complement(6203..7429)
                     /locus_tag="PK898_07170"
     CDS             complement(6203..7429)
                     /locus_tag="PK898_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOU19850.1"
                     /translation="MKKRFWLQISILLIFLTVTNGFAEDDHLSAVKTNRILYFGVAPD
                     FFPFVYHNEAGELTGLDIDLMQEIGRRMGVAVENIEIAYEGLPAALAVHQVDVIGGAL
                     EPGEMSSEMLDFSRIYFLGNYGLMILGNSEIPDSIKEEDLYWMRLGVEKGGAMDQFVK
                     TKLIGRGLIDFQQVIYFDTVAEAVDALNWNEVDLILMARNTYKRQYEIDGSYRFISAE
                     WLKENYAFASYKGSNLIPEINRLLDLIMADGTAQKLAGKYGNLISSHPIAVSPDRPEE
                     NQEEMSSAKNGEEPCLNAMRFLADVTVPDGTKIPRDSEFEKTWRVYNIGTCTWTKDYF
                     LKYKDGASLGVQRIPVVEEVRPGSDYEFTAVFKTPSETGMTRTSWQMMTPEGKSFGQT
                     IWVNIEVTDDGLSKSN"
     gene            7582..8766
                     /gene="larC"
                     /locus_tag="PK898_07175"
     CDS             7582..8766
                     /gene="larC"
                     /locus_tag="PK898_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOU19851.1"
                     /translation="MKILYCDCYSGISGDMFLAAMLDAGYPLEDLEKVFSLIELPEYQ
                     KVSFRKVQKGAVSAGQVTFVLREDPHHHTGDLIHGHSHRSFADIRKLITGSNLPESIV
                     AKSLRIFEKIAQAESSVHNVPVDEVHFHEVGATDSILDIVGAAAALDYFGIDQIFSSP
                     LPLGNGEVMTQHGLLPIPAPATAWLLRDMHAKIKPSSVAKELVTPTGAGILAAFAEFD
                     QPEMTLTHYGTGAGSADFPHPNILRVFIGDSETESESCIELSTNLDDMTPEQLGSVLN
                     RILEAGALDAFFTPIQMKKNRPAVQLSVIANRADEDKISELILRQTTTLGVRVHAVRR
                     HKADRKIVTLETEFGPVRAKTKIMNGETLSVHPEYDECERISAQTGLPLLEVQRRILK
                     HV"
     gene            9029..9178
                     /locus_tag="PK898_07180"
     CDS             9029..9178
                     /locus_tag="PK898_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOU19852.1"
                     /translation="MKRFSPYESGQGILEYVLIIILVGVIIYVLLTLLGPAITQFIQD
                     LAAPV"
     gene            9197..9922
                     /locus_tag="PK898_07185"
     CDS             9197..9922
                     /locus_tag="PK898_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOU19853.1"
                     /translation="MVNTHLRQNQKTIAWEILDWIFPPSCCGCGIKGAVICPSCRKSL
                     TLLEEPVCRICGKPFDPLDGCKTCRSQEMRFTSARAAFLYQGIVREAIHQLKYSGNLE
                     ISQVFANYLLSVYLKQNWEPDFVIAVPLSKEKMNERGFNQSEWIAKPLAKAIRKPYLK
                     GALRKKQDTDSQVGLGRTERIENLRGAFQAEPILVRGKNILVIDDVMTTGSTFNECAG
                     TLKASGAKDVFCLSVATTDSLKD"
     gene            9961..10527
                     /gene="raiA"
                     /locus_tag="PK898_07190"
     CDS             9961..10527
                     /gene="raiA"
                     /locus_tag="PK898_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOU19854.1"
                     /translation="MEKKSIIFAKNMEVSDRVEEYINKKIVKIEKFLPDVDDMKVDLA
                     YVKSARNAADRYVAQITIRGKGYILRTEERAEDIFAAFDSSLDKMLRQIDRFKGKRIR
                     GRNEKVTALTGETTGEKLEGEEEEQPLIVRRKKFNLVPMNEQEAIEQMQLLGHDEFFV
                     FYNGETASVNVLYKRHDGTYGLIDCVVG"
     gene            10816..12582
                     /locus_tag="PK898_07195"
     CDS             10816..12582
                     /locus_tag="PK898_07195"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU19855.1"
                     /translation="MAEKNDQYHLITEYLKVSIPADPAGCIEITNHLFDLTISSYEIW
                     INGKKIDLSLCGKDICFPLFSEMGSTMRLPAPMPMSDQDDVKIGLNTVEIGRLTTGNR
                     IPAPEKPQSKHKWRVLRLFARDFDEDLMVDSQAIIGRRENQTLGFSFFPFDNRQTYSL
                     WFNSENAELQPAVVSMLKDIRPQSLGFRLPVDFGRMEADGTALKCFLKLCKTLEASPH
                     IRIDLNHYCGREITEVAQSAFGSLRSEDWWKPVWYFDFFVGDGFESGISAEETADKIN
                     DLSERLRSLNPDIKIILTGADPSLGSAVRWNEEMVTKCCRKIDIFGIQWLFPGVKGWL
                     RGKGPFETEFATGYSAALDQALRITHGMLQNASPDQSIPVALSCWSYLRAPSQNTADY
                     RINFSKQDCFFHASQLNSIIRNSDFVSLAENGPLIGNLGLIQAEGDRIWGSAAYHLQK
                     LYLDSREIVLKVATIRDKPMQTYSWSGIPGLIEGRDIPYIDVLATRSADASNLTLLVL
                     NRHHRKRAHVRVSFRNFADLRPVEAKILRSGKFSFQNTFSKPETVYCASIPLGKYKAM
                     DHVNLDLPPYGIISMTLTASNR"
     gene            12784..13521
                     /locus_tag="PK898_07200"
     CDS             12784..13521
                     /locus_tag="PK898_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19856.1"
                     /translation="MFHKCKAVFFISLCLIIACLSSMGPAFYTDESEDSDLIVDGVFE
                     EKFQDDFWDRGWQKLILFGNPEGNYYSLNRKRLSLELPSTQTSVVLTNPNVNFSDVMV
                     EATFENILSNVASYAVICRYSEDGWYELRVNISGPEAGSFKLSKYDFYLKEQYKNPYV
                     LLHKGMDRIFSVDIKLGLNVKNKLGLLCKGDEIKAYINDKEQFPIKNGKITDDQFEEG
                     SVGFSAQTYGEGLAKIDIVKFSAKEVQ"
     gene            13807..14364
                     /gene="efp"
                     /locus_tag="PK898_07205"
     CDS             13807..14364
                     /gene="efp"
                     /locus_tag="PK898_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOU19857.1"
                     /translation="MIDVNELRKGVTFEIDGELLKVLDYEHNKSGRGNASIKVKARNL
                     RTGATYERTFTSGNRVQDVQLDFETVQFLYADSDYFHFMNTETYEQPEVRAEIMKDAA
                     PYLKEGTEVKLTLYQNEIIDYELPMSVDLKVIQADVAVRGDTATGVSKQVTVETGARI
                     SVPNFINEGDTIRVNTSTGEYLTRV"
     gene            complement(14434..15627)
                     /gene="ygeW"
                     /locus_tag="PK898_07210"
     CDS             complement(14434..15627)
                     /gene="ygeW"
                     /locus_tag="PK898_07210"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOU19858.1"
                     /translation="MKKSKKLLEELKKLDFHKMYNEDFLLTWEKSDDEIAATFKVADI
                     LRDIRENNISPRLFDSGLAVSLFRDNSTRTRFSYASAANLLGLEVQDLDEGKSQVAHG
                     ETVRETANMISFMADVIGIRDDMYIGKGNAYMREVSKAVEQGYKDGILEQRPTLVNLQ
                     CDIDHPTQSMSDMLHIINTFGGVENLKGKKIAMSWAYSPSYGKPLSVPQGIIGLMTRF
                     GMEVVLAHPQGYEVMPEVEKVAKKNAKASGGSFKVTNSMAEAFENADIVYPKSWAPFN
                     AMEKRTELYGQGDFDGIKALEKELLAQNAEHKDWECTESLMKTTANGNALYMHCLPAD
                     ISGVSCKEGEVESSVFDRYRTPLYKEASYKPYIIAAMIVLSKAKDPVAWFEKLTDKTP
                     KRFLA"
     gene            complement(15664..16878)
                     /locus_tag="PK898_07215"
     CDS             complement(15664..16878)
                     /locus_tag="PK898_07215"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOU19859.1"
                     /translation="MNIDYKAIKAKAESYADDMTKFMCDLIAIPSESCNEKGVIYRIK
                     EEMEKVGFDKIEIDPMGNILGYIGHGSHLIAMDAHIDNVGVGEIKNWTVTGPYEPKVI
                     GDAIYGRGGSDQEGGMVSMVYAGKIIKDLGLEDDYTLLVVGSVQEEDCDGLCWQYIIN
                     EDKIRPEFVLITEPTSLRIYRGHRGRMEIKVHTHGISCHGSAPERGDNAIYKMAQIIE
                     EVRALNENLHYDPFLGKGTVTISEIFCTTPSRCAVADGCTISLDRRLTNGETWEGALQ
                     EVRNLPGVKMADAEVSLYTYERPSWTGLTYPTECYFPTWVIPEDHPTTKTLVEAYEGL
                     YEKEPVVDKWTFSTNGVAIMGRYGIPCIGFGPGAEEEAHAPNEKTWTSHLVQAAAMYA
                     AVPKLYVNKYAK"
     gene            17234..19105
                     /gene="ftsH"
                     /locus_tag="PK898_07220"
     CDS             17234..19105
                     /gene="ftsH"
                     /locus_tag="PK898_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU19860.1"
                     /translation="MKQRGQTNIVYLLLLVGIISLLLYSFTGNRSANNVISINQLANQ
                     IKQGNIAKIESNSTTVNILYKDRSVAKAVIEPNSSLLEQLTLLGVTPEELSSDNIEIS
                     IVEESSWVGILSILSYFLPVIFLALAMFFIFRQTQSGNNSALSFGKSRAKMFEGDHPT
                     VTFADVAGVDESKEELQEIVEFLKEPQKFTRLGARIPKGVLLVGSPGTGKTLLAKAVS
                     GEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKKNSPCIIFIDEIDAVGRQRGAGL
                     GGSHDEREQTLNQMLVEMDGFETDTNVIIIAATNRPDILDPALLRPGRFDRQVVLDRP
                     DVVGREAILKVHSKGKPLDKDVDLNALARATPGFVGADLENLMNEAAILAAKRNKNSI
                     GNAEMEEAVERVIAGPQKKSRILTEKEKRIIAYHEAGHAIIMNVIPECDPVHKVTIIG
                     RGSAGGYTMHLPTEDLVLMSKKQIEAEMVSLLGGRAAEELMFDDITSGASSDIERVTE
                     LARSMVTRLGMSEVMGPIVYGKKEEMIFLGREISEQRDYSEAVAEDIDAEVRKIVNSA
                     FEKAKALLVEYREYLDKIANKLIEVETLTKEEFEAIFPPPNGKVSATPNYLEQKQAV"
BASE COUNT         5225 a         4517 c         4253 g         5209 t
ORIGIN      
        1 gcatctccaa aaattatcgg gaaaatcaat aaaaataaaa tccgtttttc ggtgatccac
       61 cggttcggct tatgcgaata tgtaatgtct tcacagattt tactgagttc ggcttcaatg
      121 ccgaaaatgt cgaggatcat ccgtatcgtc ataattgcgg tagattttat ctcccgccga
      181 taaatcgttt ttttgtggtt gtgattccgg ataaacgcaa caggtaacgc aagcatattc
      241 tcggaatctc tgtacggatt ataccgcaaa actttatcga gaattcgcag tatttcctcc
      301 gcaaaacact tgagtgctga tccgctaaat tattgaaaac agaggcaaca ggttttccaa
      361 accgtcgcct tccggaatac cggtttgctt caacatccgc agccgcagaa caaaatgtca
      421 cggtgcggga tttccggccc gcgattcaaa ggatagaaca actccgaacg aaattcccgg
      481 gagaaaaacc gcttgtgcgc gttgaacgaa tactcgtaag gtaggcttgt agttttattt
      541 gaagagccta taaatatctc ggagttttac cgaataacat caaaattgtc agccggccga
      601 aatccgggtg gatcatccat gcccggtagg gaaaaaatcg taatcggatc tcgatacaga
      661 tttccgacgg atttcgagtg ggtttgtata tccggtgtca aaccgccgat gaaccctgct
      721 caggtccata acggcgattt tcggcaagat ttttttctgt ctatcgaaaa attatgaatc
      781 aaaaaaccat ccgtcgacaa gtttgttcct gtcaacagac ggttttcaat ctggcggaga
      841 gagtgggatt cgaacccacg ttaccttgcg gtaaacgcgc tctccaggcg cgcgcgttag
      901 gccagactac gctatctctc cctaatgttg cggacgctat tataccataa ggtaaattat
      961 tttaggaaaa ccccaagatt tcagatagaa aataacctgt tttataaaag tgtagactat
     1021 aatcgaatta tgtacatcca gaattcgcaa attccgcagc cgatcctgtc tgtatcacaa
     1081 aagaccctcg atttcggact tgtctcagcc ggtgaaccgg cagagggaca cggtatcgcc
     1141 aaattgacga ttacgaatga cggagatcgg attctggtcg ggaggattgc gattcaggtc
     1201 gcttgggtta ccgttgaccc gcccgatttt cgcttgaacc cgggtgaaag cagcgaacat
     1261 atttttacgg tgcgccaaaa cgttcagttc tcttggacga ctcggcgaat gggttcggat
     1321 tttatcgccc tcatcaacag taatggcggc tccgaaacga tcggcggata ctattttctc
     1381 gattcgacaa aaacggagaa aaaaaagaag ccgattcatc cttggttata tgcactcatt
     1441 ccgattttca ttttgctgac agccggaatc attctttttg tttcgcgaca aaatgaggcg
     1501 gctctggcac agcaaaaaac ggaaaactcc gattcgctgt atacccggat tgcggaaacc
     1561 tattttgccg aaatgacaga aaatccgacc attatttcca ccgaacctcc tccgcaaaat
     1621 acccccaatc cgctggttgt tccgaatttc gatatcactc cttctcccgc tccttcgatg
     1681 acgtttactc cttgggtcgc ggcagactat ccgaatgtcg aagaattcat ccgcacttac
     1741 tacaaaacgc tgaatgaacg agactatgag accgcttggt ggatgctttc ggaaaaaatg
     1801 caacaggctt gctgttacac gggagaggca aacccgatga cggtttatca aaattactgg
     1861 gataaagtcg aatccgttga ggtaaattat gcgtttctcc agagctatga taccaatccg
     1921 gcaatcgtaa atgtcggctt gacttatcac tataaagacg gaacgaccga ggaaacggtc
     1981 aatcagtttt cgattatcac agatgatgtc aaaaagagtt tggtgatcga tgaaatcagg
     2041 taaaattttt atcagtcagg tgagtgttaa aatcgataac aaaatataaa ttgagaaatt
     2101 tattcaggag aacgaattat ggaaataact ttggataatg taaaaaaagt gattaccgat
     2161 gtattaaaaa tcgacggatc ggatatcact cccgaaaccc gttttatcga agaccttaaa
     2221 gcggattcaa tggatcagtt tttcctgatc gacgggttca gcgaagcttt taatgtgacc
     2281 atttccgacg aacaggcgaa ggaaattaaa accgtgggag atgtcatcag agttctgtcc
     2341 aaataaatcg aaaaaggctg ataaacagga acatctgcct gtcttctgca atgttcctgt
     2401 ttttttattt aaccctgtaa ggcgtcgggg acttctttta tatcggtgac cacacggaca
     2461 ccgaccgatt cgaacgcttc gattttagag tcgataccgc cgattccggc ttcgatgaag
     2521 gcgccggaat gtcccatctt tttcccctgc ggagcagtct tccccaccaa caatgcggtg
     2581 acgggtttgg tcattttata ggcgacatag ctcgccgcac gttcctcttc caatcccccg
     2641 acttcaccga ttaataggat tcgctcggta tatggatcgg tctcaaacag ttccaacgct
     2701 tccacaaagg tagtcccctg aacaatatct ccgccgatcc cgatacaggt actgacaccg
     2761 attccccgac ttttcatttc actcatcacc aaatagctga gcgttccgga acgcgagata
     2821 acgccgactt ttccgggaat cacacaatcg gacggcgcta cccccaattt ggcttccccc
     2881 gggttgataa tgccggaaca gttcggtccg accaatcgca ctccccggtt ggacatatat
     2941 tggcacactt ttaccacatc ctgcaccgga actccttcgg tgatacaaac caccaattcc
     3001 acgcccgaat ccgccgcctc aaacagtgca tcggtggcgt ttcgcgccgg cacgaagatg
     3061 atcgatgcat tcgcttccgt gacttccatt gcagcctgca tggtatcgaa taccgggatt
     3121 tttccgtcca gcacccagct tccgcctttt tgaggcgaga cacccgcaac aatttgagtg
     3181 ccgtaagccg ccatttgttc cgtatgaaag ctgccttccc tgccggttat tccctgaaca
     3241 atgaccttcg tcgatgaatt aatcaaaatt cccatatcaa ccctgtcccg cagctgtttc
     3301 gaccgctttt cggatcgctt cttcataact cggcaccaaa atcgccggag agccgctcaa
     3361 gatttgattc cccaatgccg acatcgaccc acccatatag acaacgatgg gaactttgct
     3421 gtcatatcga ttcatcgctt ccagtaatcc gcgagccacg tcatcgcaat gcgtcattcc
     3481 cgcgtaaata cataccaaga tcactttcac cgccgggtcg ttttgcagta tatccatcgc
     3541 cgccaggatt ttttcggtat taacccccgc gcccaggtca agaaaatttg ccggtttccc
     3601 gccgtttgct tcaatcagat ccagcgttgt catcgtcaaa cccgccccat tgatcatgca
     3661 ggcgatattc ccgtccatct taatataatc caggccgtgc ttgccggctt ccaattctcc
     3721 ataccttaaa taaccgagat cgacaaaatc ggtcagcgat tcctgccgaa aacgggcatt
     3781 ttcatcgagt gtaattttgc cgtccagcgc caaaatccgc tgatcggtgg ttatcacaag
     3841 cggattgatc gctgccatcc gggcatccaa gtcgcaaaac agacgccaaa gtcccataca
     3901 gatttcggtc agattgcgaa tatatttacc cggaaaatcc agtttgtaag ccaactgatg
     3961 aatctgataa cgctgcaaac cgatcaacgg atcgatgtaa acccgatcca aattttcaaa
     4021 caaagcgttt gcggctgcct cgatataaat cgttcccgaa tcatacatca tcagtagcgg
     4081 tcgttttttt gcccgatcga tggaaatcaa aataaaatat tctttttcga ttgagagcgc
     4141 ttcgtcaatc aggactttct gaacgggaag cccgtgaatc ttcgccgaaa gaatttcgga
     4201 ggcaaaaatc tctgtctctt cgggggtttt ggctaaacga atcccgccgg ccatcccccg
     4261 gccctcggct aagacttggg ctttgacgac gaccggcaat ccgatttctt cggcaatttg
     4321 ttttgcttcc cgtgcgattg atgtaacatg tccggccgga atcggtattc cgtacttttt
     4381 aaagaaacct ttagactgat actcataaag tttcattgtt ttttggttgt ccgcttatcc
     4441 tttcagaaat tcattcagag attcccgact gattcgataa gctgagccga tttttcgtgc
     4501 tttcaattct cccgattcga tggaagcgat aacatcctct tccgatactt tcaataattt
     4561 tgccgcttca gcgggagtca atacttcacc ggattccgcg gttgccggtt gatcggcagc
     4621 ggctgaagcg ttactttgtg cagggggttg gttgcgatac gtatttgcga taatatcccc
     4681 caagccgata ccggcaccga ttccggcggt taatcccgca ccgccgctcg gatttttcgc
     4741 agcctcgcgc agtgcatccg ccgcttgaag ttgggtatag acgtccatgt cgagcattcc
     4801 catcgcccgc agctcttccg ccgatttttc ggagggtttc aaactggcga tataaaaact
     4861 cttcagcgcc aacccgatgg cggcaaaatc atcttgagct ttcgcccgga cggccgcacc
     4921 gatctcttcc accaacccaa tcagcttcat cacatccgca ttttgttccc cggttatcac
     4981 acccaaggta tcctgcaatt tggagagcag catcgctcgc agccgatctt cgatcgcact
     5041 caacgaatag gattgtgaag cgccgacgat ctgagtgacg aactgctgcg gatcactcac
     5101 ctgaaaactg taggtcccat acccttgcaa taaactgatg cccagcccca cgccggggtt
     5161 ccgaacgatg atcggttgtg aagtccccca tttgacatcg gcgaattccc gcatagagac
     5221 aaaataaatt tctgccgtaa acggcgtccg atgattaaat gcaacgccca acaaagcagt
     5281 taaaagcgga atgttagccg tagaaattgt atggcgtccg ggtcccaatt gatccagcgc
     5341 ctttccgtct ctgtaaaata tcgccctttg ggattcgcga acgatgacct gagaaccgaa
     5401 tcttaaatcg gccaccccgg tctcggggaa acggtgaaca atttcatccg tcatttcact
     5461 tggatattca actacatcaa aaatgcgagc cattctttat tctcctgatt ttcccgaatt
     5521 aagatcactt gcaccgatta tgacttcggt acgctggtta aatgccgtca ggctatcctg
     5581 cgcggctttt gtcaactgac tgatcgcgtt tttaatatta tccggatcgg atgcgaccgc
     5641 tttttccact tcatctacag aagctttgac ggtatccgcc atcttcagta agtacaaatc
     5701 ataatcatat agccgtgtca aggcggcgtc atccactttt atggcatcca acaatccggc
     5761 gtatccatag ggggctgtct tgattcgatc ggtaaattgc cgaattttaa tcgccgcact
     5821 ttccaaatcg gaaaccgcat tgagatttcc ggctttaatc aaatcacgtt gaatcgccga
     5881 aatcctggac cattcttcct cgaatttttt ggcaatttct tcacgaataa ttttgtctgt
     5941 atcacggcga tcttctttcg ccttatatcc cttaatccca gggatttttt ccattaattc
     6001 ctggaaactg ttcttcccgg acttgacgag gtcatttaaa ttttccattt atcctcctta
     6061 tcttccatcc ccgcatattt ttcttttaaa ttgttcattc tttcgataat caataatttc
     6121 cgtaaatcgc tgcctgttaa gacagccgtt cttcccaatt ttaccttatc cgtcgcagac
     6181 aaaactcgaa ccaagcgtcg tgtcaattgc tttttgaaag cccgtcatcc gtaacctcaa
     6241 tattgaccca aatggtttgt ccgaaggatt ttccttcggg agtcatcatc tgccagctcg
     6301 tccttgtcat acccgtttcg gagggggttt taaagacagc ggtaaattca tagtcggaac
     6361 cggggcgaac ttcttcgaca accggtatac gttgtacgcc cagtgacgct ccgtccttat
     6421 atttcagaaa ataatctttt gtccaggtgc aggttccgat gttgtagacc cgccaggttt
     6481 tctcgaattc gctgtcgcga ggaatttttg tcccatccgg aaccgttaca tcagcaagaa
     6541 aacgcatggc attcagacag ggttcttctc cgtttttcgc agagctcatt tcctcttgat
     6601 tttcttccgg acgatccggc gaaaccgcaa tcggatggga agagattaaa ttcccgtact
     6661 taccggcgag tttttgcgcc gtgccatccg ccatgatcaa atccaataac cgattgatct
     6721 cgggtatcaa attcgaacct ttataagaag caaaggcata attttccttc agccattcgg
     6781 ccgaaataaa gcgataagat ccgtctattt catattgtcg cttataggta ttccgagcca
     6841 tcaaaatcaa atccacttca ttccaattca aagcatcgac cgcttccgca acggtatcga
     6901 aataaatcac ctgctgaaaa tcgatcagtc cgcgtccgat cagtttggtc ttgacaaact
     6961 gatccatcgc accgcccttt tcaacaccga gacgcatcca gtaaaggtct tcctctttga
     7021 tcgaatcggg gatttccgaa ttccctaaga tcatcaatcc ataattgccc agaaaataaa
     7081 ttcgggagaa atccaacatt tcgctcgaca tttcaccggg ctccaatgca ccgccgatca
     7141 catcgacctg atgaaccgcc agagcggccg gcagaccttc ataagcgatt tcgatgtttt
     7201 cgaccgcgac gcccatccgg cgcccgattt cttgcattaa atcgatatcc aatccggtca
     7261 attcaccggc ttcgttatga taaacaaaag ggaaaaaatc gggggcgact ccaaaataca
     7321 ggattcgatt tgttttcact gccgacagat gatcatcttc cgcaaacccg ttcgtaacag
     7381 tcagaaagat cagcaaaatt gagatttgaa gccagaacct ttttttcaaa accagctcct
     7441 tttcttaaat cgaaacccgg aaaaatccgc tcaaaaaaag acattcataa ttagattata
     7501 gcattttgca ctaaatcagg ttaacattat gcgaaataaa agaaatcggc agcaacaaat
     7561 atctttgatc aaggactcgt tatgaaaatt ctttattgcg attgttattc cggaatcagc
     7621 ggtgacatgt ttttggcggc gatgttggac gccggatacc cattggagga tcttgaaaaa
     7681 gtcttttcgc ttatcgaact tccggaatat caaaaagtca gcttccgaaa agtgcaaaaa
     7741 ggagccgtca gcgccggtca ggtaacgttc gtcctgcggg aagatcccca tcatcatacc
     7801 ggtgatctca ttcacgggca ttcgcatcgt tcctttgccg atattcgcaa gctgattacc
     7861 gggagcaatc ttcccgaatc gattgtcgcc aaaagtctgc ggatttttga gaaaatcgcc
     7921 caagcagaaa gttcggtcca taacgttccc gtcgatgagg ttcacttcca tgaggtcggt
     7981 gcaacggatt cgattctgga tatcgtcggc gcggcggctg ccctggatta tttcggaatc
     8041 gatcaaatct tttcctctcc gctgcctctc ggcaacggag aagtcatgac gcaacacggt
     8101 ctgttgccga ttccggctcc cgcaaccgct tggttgttac gggatatgca tgccaaaatt
     8161 aaaccttctt ccgtcgccaa agagctggtt acccccaccg gagccggcat tttggctgcc
     8221 ttcgccgaat ttgaccaacc ggaaatgaca ctgactcatt acggcaccgg tgccggttcg
     8281 gcggattttc cccacccgaa cattttgcgt gtgtttatcg gggattccga aacggaatcc
     8341 gagtcctgca tcgaactctc caccaatctg gatgatatga caccggaaca attgggatcg
     8401 gttttaaatc ggatcctgga agcgggagcg ttggatgcat ttttcacgcc gattcagatg
     8461 aaaaaaaatc gcccggccgt tcaattgtcg gttatcgcca accgtgcgga tgaggataaa
     8521 atcagcgaat taatcctgag gcagacaacc acgttgggcg tacgcgttca tgccgttcgc
     8581 agacacaaag cggatcgcaa aatcgtcact ttggaaacgg aattcggacc cgtccgcgcg
     8641 aaaacaaaaa tcatgaacgg ggagacgctt tccgttcatc ccgaatatga tgaatgtgag
     8701 cgtatttccg cacagacggg acttccttta ctggaagtgc aacggcgaat cttaaaacac
     8761 gtttgatcct gttcaaaagc tgatacaggg atgaacaccg acgggataca gggagttgtc
     8821 gcctgagaag ctgaaatgtt tcttaaatca ggtgcctcat gtttgcgaat cttcggcatt
     8881 cgagcatcca gatatccgat gggatagtcg attcttttca tcgattggaa atgagcgaaa
     8941 acggacggac ggattgacaa cccttattaa aaaagaatac aataagaaaa aggcaccata
     9001 atttattacc ggacaaaggg taaacaatat gaaaagattt tcaccgtatg aaagtggaca
     9061 gggaatttta gaatatgtgt tgatcattat cttggtcgga gtaatcattt atgtcctgct
     9121 tacacttctc ggaccggcta tcactcaatt catacaagat ttggcagcgc ctgtttaaaa
     9181 gggataatat gcgggaatgg tgaataccca tcttcggcag aaccaaaaaa caattgcatg
     9241 ggaaatcctg gattggattt tcccgccgtc atgctgcgga tgcggaatca aaggagcagt
     9301 catttgcccg agctgccgaa aatccttaac tttgctcgaa gaacctgttt gccgaatttg
     9361 cggaaagcct ttcgatccgc tggatgggtg caaaacctgc cggtcgcaag agatgcgttt
     9421 tacttccgcc agagccgcct ttttatatca gggtatcgtc agagaagcga tccatcagtt
     9481 aaaatattcc ggcaatctgg agatttcaca ggtatttgca aattatttat tatctgtata
     9541 cttaaagcaa aattgggaac cggattttgt aatcgccgtt ccgttgtcaa aggaaaaaat
     9601 gaacgagcgc ggatttaatc agtccgaatg gatcgccaag ccgctggcaa aagcgattcg
     9661 gaaaccgtat ctgaagggag ccctccgcaa aaaacaagac accgattctc aagtcggatt
     9721 gggccgtacg gagcggatag agaacctgcg gggagctttt caggcggaac ccatcctggt
     9781 gagaggaaaa aatattttgg taatcgatga cgtgatgacg accggctcaa cgttcaacga
     9841 atgcgccgga actttaaaag catccggtgc gaaagacgta ttttgcttaa gtgttgcgac
     9901 gacagattca ttaaaagatt gaagtacgaa taattattta ttcaggagga acacaagctc
     9961 atggaaaaga aatctattat tttcgcaaag aacatggaag tttctgatcg ggttgaggaa
    10021 tatatcaata agaaaattgt aaaaattgag aaatttcttc ccgatgtgga tgacatgaaa
    10081 gtcgacctcg cttatgtcaa gtcagccaga aatgccgccg atcgatatgt cgctcagatt
    10141 accatccgcg gcaaagggta tattcttcgt acagaggaac gcgccgaaga tatttttgcc
    10201 gcttttgaca gttcactgga taaaatgctt cgtcagattg atcgtttcaa agggaagcga
    10261 attcgcgggc ggaatgaaaa agttaccgcg cttaccggag aaaccaccgg agaaaaattg
    10321 gaaggggaag aagaggagca accgcttatt gtccggcgta agaagtttaa tctggtgccg
    10381 atgaacgagc aggaagccat cgaacagatg cagctcctcg gacatgatga attctttgtc
    10441 ttttataacg gtgaaaccgc ttcggtaaat gtgctctata aacggcatga cggcacttac
    10501 ggattaattg attgtgttgt cgggtagtaa accgaattga tcaccgatca tcaaggcgcc
    10561 tccggacgga agcgcctttc ttttttaaac cgattcggtt ttcggaaata aaaatcgggc
    10621 aaattttgtc gaaaacttcg gatcgatacg acaggttgtc caaattcaaa cgcttatata
    10681 aaaatgatcc ggtgcctgaa gtcttcctta ttgattattg cggacatttt atgatcggcg
    10741 accgatctca aagccggacc aaatctcctc gattcgtcgt tttttcggtt aaacttaaaa
    10801 aaggggagcc gccatatggc tgaaaaaaac gatcaatatc atctcattac cgaatattta
    10861 aaagtctcga ttccggcaga tcctgccggt tgtatcgaga tcacaaatca tctgtttgat
    10921 ttgacgattt catcttatga aatctggatt aacggaaaga aaattgatct ctcgctttgc
    10981 ggaaaagata tttgcttccc gctcttctcc gaaatgggaa gtaccatgcg ccttcccgca
    11041 ccgatgccca tgtcggatca agacgatgtt aaaatcggtc tgaatacggt tgaaatcgga
    11101 cgattaacga cggggaaccg tattccggca ccggaaaaac cgcaatcgaa acataagtgg
    11161 agagttttac gtctctttgc gagagatttc gacgaagatc taatggtcga ttcacaggct
    11221 ataatcggcc ggcgtgaaaa tcaaacgttg ggattctctt ttttcccttt tgataatcgc
    11281 caaacctatt cattatggtt taattccgaa aacgcagaac tgcagccggc ggtcgtatcc
    11341 atgctcaaag atatcagacc tcaatcgttg ggatttcgct taccggtgga tttcggacgt
    11401 atggaagcgg atggcacggc gttgaaatgt tttttgaaat tatgcaaaac gcttgaagct
    11461 tctccgcata tacgaatcga cctcaaccat tattgcggca gagaaatcac ggaagttgcg
    11521 caatcggcat tcgggtcact ccggtctgaa gattggtgga agcccgtttg gtattttgat
    11581 ttctttgtcg gggacggctt tgaatccggt atttccgcgg aagagactgc agacaaaata
    11641 aatgatcttt cggaaaggct tcgttctctg aatcccgaca taaagataat tttgacgggt
    11701 gcggatccgt cattgggatc ggccgttcgc tggaacgaag agatggttac aaaatgttgc
    11761 cggaaaatcg atatctttgg gatccaatgg ttgttcccgg gggtaaaagg atggttgcgt
    11821 ggaaaaggcc cgtttgaaac agaatttgcc acgggatatt ccgccgcgct ggatcaggca
    11881 ttgagaataa cgcacggaat gctgcaaaat gcttctccgg atcaatcgat tccggttgcg
    11941 ctgagttgtt ggtcctatct cagagcaccc tcacaaaaca ccgccgatta tcggattaat
    12001 ttttcaaagc aggattgttt ctttcatgct tctcagctca acagcattat ccgaaattcc
    12061 gattttgttt cgctggcgga aaacggtcct ttgatcggaa atttagggtt gatacaggct
    12121 gagggagatc gaatttgggg atccgccgca tatcatttgc agaagttgta tttggatagc
    12181 cgggaaatcg tcttaaaagt tgctacgata cgagacaaac cgatgcagac ctattcttgg
    12241 tccggtatac ccggtttgat cgaaggaaga gatattcctt atattgatgt tcttgcgacc
    12301 cgttccgcgg acgcgagcaa cctgacgctg ttggttctta accgtcacca tcgaaaacgg
    12361 gcgcatgtac gggtcagttt caggaatttt gcggatttac gaccggtgga agcaaaaatc
    12421 ctgcgctcgg gaaaattttc gtttcagaat accttctcca aaccggagac tgtctattgt
    12481 gcttccattc cattggggaa atataaagcg atggatcacg tcaatttgga tttacctccc
    12541 tacggaatta tttccatgac attaaccgct tcgaatcggt aagcttttaa cctcgaaaac
    12601 cctcttcgta tactcaagcg gatttttcgg ctattcggtg cctttgattg ttctacattt
    12661 tcaatcgctc cgccgaatat ctggacaact gttgaaaaaa tggtataaat tatttacctt
    12721 tatatttaac ttccttcaga agtttgtaac gtcaatcgaa tcgttttaga gataagaggg
    12781 gatatgtttc ataaatgtaa agccgtattt tttatatcgc tttgcctgat cattgcctgc
    12841 ttgtcctcaa tgggcccggc attttatacg gatgaatccg aagattccga tctgattgtc
    12901 gacggggtat ttgaggagaa atttcaggat gatttttggg atcgcggttg gcagaaatta
    12961 atcctcttcg gaaatccgga aggaaattac tatagtctga atcggaaacg attatcgttg
    13021 gagcttccat cgactcaaac ctccgtagta cttaccaatc cgaacgtgaa tttttccgat
    13081 gttatggtgg aagccacttt tgaaaatatt ctttccaatg tagcctccta tgccgtaatt
    13141 tgtcgatatt cggaagacgg atggtatgaa ttacgcgtga atatctcagg gccggaagcc
    13201 ggatcgttta aattatcaaa atacgatttc tatctgaaag aacaatacaa aaatccatat
    13261 gtattgttgc ataagggaat ggaccgtatt ttttcggtgg atattaaact gggtttgaat
    13321 gtgaaaaaca aattggggtt actttgtaaa ggagacgaaa taaaagcata tattaatgac
    13381 aaggaacaat tcccgattaa aaacggcaaa attaccgatg atcaatttga ggaagggtcg
    13441 gtcggctttt cggctcaaac ctacggcgaa ggattggcaa agattgatat tgtaaaattt
    13501 tccgcgaagg aagttcagta agatcttccg ttaagttaca cataagatac acaaaaaaga
    13561 gtgcaaaaat ttttgcgctc ttttgttaag aaaacacgtt atcaaatcat atatagtgct
    13621 ttggaggaaa taaactgtcc tccgaaagcg aaaagttctt tattccggtt cgccgcaagg
    13681 acagcattca ttcatgtttc aagcggctga tcgtcgatta tgccaattta ctgatataat
    13741 ctaaaagcta cggaaggaac aagttctttc aagcataaaa gagactgaat acagaaggat
    13801 ttttttatga tcgatgtaaa tgaattacga aaaggtgtca catttgaaat tgacggcgaa
    13861 ttactgaagg ttctggatta cgaacacaat aagtcgggtc gcgggaatgc aagcattaaa
    13921 gtaaaagctc ggaacctgcg caccggcgcc acatatgaga ggacattcac ttcagggaac
    13981 cgagttcagg atgtacagct tgattttgaa accgttcaat ttctttatgc ggattccgat
    14041 tactttcatt ttatgaacac cgagacttat gaacaacctg aggttcgggc ggaaattatg
    14101 aaggacgccg ctccttactt aaaagaagga accgaagtca aattgaccct ctaccaaaat
    14161 gagattatcg attatgaact gccgatgagc gtggacctga aggtgattca ggcggatgtc
    14221 gccgttcgcg gggatacagc gacgggagtc agtaaacagg taaccgtcga aaccggtgcg
    14281 cgaatcagcg ttcctaattt tatcaatgaa ggggatacga tccgcgtgaa caccagcacc
    14341 ggtgagtatc tgacaagagt gtaagatatt ccgagaaatt caagaaaaaa gccatcgacg
    14401 catcgatggc tttttcatat ccttggatcg gcttcaagca agaaaccgct tgggagtctt
    14461 gtccgtcagt ttttcaaacc acgctaccgg atctttcgct ttgcttaaga caatcatggc
    14521 agcgatgata tagggcttgt aactggcttc tttatacaga ggtgtccggt aacgatcaaa
    14581 caccgaactt tccacttcac cttccttaca gctcacaccg gaaatatcag ccggcaggca
    14641 gtgcatatat aaggcatttc cgttggcggt agtcttcatc agactttctg tgcattccca
    14701 atccttatgc tcggcatttt gtgccaacag ttctttttcc agcgctttaa ttccgtcaaa
    14761 atcaccttgc ccgtacagct cggtgcgttt ttccatcgca ttgaaaggtg cccagctctt
    14821 cggatagacg atatccgcat tttcgaaagc ttccgccatc gagttggtca ctttaaagct
    14881 gcccccgctc gctttggcat ttttcttcgc aaccttttcc acttcgggca tcacttcata
    14941 accctgcgga tgagccagaa cgacttccat accgaaacgg gtcattaaac cgataattcc
    15001 ctgaggaacc gaaagcggct tcccgtaact cggtgaatag gcccaagaca tggcaatttt
    15061 ctttcccttt aaattttcga ctcctccgaa ggtattaatg atatgcaaca tatccgacat
    15121 actttgggtc ggatgatcga tatcgcactg caaattgacc aacgtcggcc gttgttccaa
    15181 aataccgtct ttatatccct gctcgacagc cttgctgact tcacgcatat aggcatttcc
    15241 cttgccgata tacatgtcat cccggatccc gattacgtca gccataaagg aaatcatatt
    15301 ggctgtttcc cgcaccgttt ctccatgagc gacctgcgat tttccttcat ctaaatcctg
    15361 aacttcaagt cccagtaaat ttgcggcgct ggcataactg aaacgggtac gagtggagtt
    15421 gtcacggaat aaagaaacgg ccaatccgct gtcaaataat cgaggggaga tattattttc
    15481 gcgtatgtca cgcaaaatat ccgcaacttt aaaagttgcc gcaatttcat catccgattt
    15541 ttcccatgtc agcaaaaaat cttcgttata catcttgtga aaatcaagct ttttcagctc
    15601 ttccagtaat ttttttgact ttttcattga tcattatcct ccgaataaca aataatcaaa
    15661 attttatttt gcatacttat tgacgtatag cttcgggacc gccgcataca tcgccgcagc
    15721 ctgaaccaaa tgactggtcc aagttttttc attcggcgca tgagcttcct cttccgcccc
    15781 cgggccaaat ccgatacagg gaatgccgta acgtcccata attgcgacac cgtttgtcga
    15841 aaacgtccat ttatcgacca ccggttcctt ctcatataat ccttcgtaag cttcaacgag
    15901 agttttggtg gtcggatggt cttcgggaat cacccacgtc gggaaataac attcggtcgg
    15961 ataggtcaaa ccggtccaag acggccgttc ataagtatat aatgaaactt ccgcatcagc
    16021 catttttacg cccggaagat ttcgaacttc ttggagtgcg ccttcccaag tttccccgtt
    16081 ggtcaacctg cgatcgagag aaatggtaca gccatccgca accgcgcaac gtgagggggt
    16141 cgtgcaaaag atttcggaaa tggtaaccgt cccttttccc aagaagggat catagtgtaa
    16201 attctcattg agcgctcgga cttcttcaat aatctgagcc attttataga tcgcgttatc
    16261 accccgttcg ggtgcggaac cgtgacagct gataccatgg gtatgaactt ttatttccat
    16321 tcgaccgcga tgtccccgat aaattcgtag tgatgtcggt tcggtaatca gaacaaattc
    16381 gggtctgatt ttatcttcat tgataatgta ttgccagcat aaaccgtcgc aatcttcttc
    16441 ctgcacagag ccgacaacga gcaatgtgta atcatcttcc aaaccgaggt ctttgatgat
    16501 ttttcccgca taaaccatgg aaaccatccc accctcttga tccgatccgc cgcgtccgta
    16561 aatcgcatcg ccgatcactt tcggttcata cggaccggtc accgtccaat tttttatttc
    16621 gccgactccg acattatcaa tatgggcatc catcgcaatt aaatggctgc cgtgaccaat
    16681 gtagcccaga atgtttccca tcgggtcgat ttcgatttta tcgaagccga ctttttccat
    16741 ttcctcttta atccgataaa tcactccttt ttcattgcag gattcactcg gaatggcaat
    16801 caagtcacac atgaatttcg tcatgtcatc agcgtagctc tcggctttag ccttgatcgc
    16861 tttgtaatct atattcattt tttcctccaa aatatattcg ataagtggcc gaaaccaaaa
    16921 ttaaccataa aacatacaga cggaatttaa ttgtcttaca attaaacaca aactttagga
    16981 tattttacac tatggttgaa aattaacggt tattttttgt caatttcttg tacttttatt
    17041 tgtatgacaa attacgccgg atcgttttca gccttttttt ctgaaattcc ggggcctcgc
    17101 tttcaccatt ttcagaaaaa aagaagcatc tcattataat taaatttgaa agaaatttat
    17161 taacataatc aattaatggg aacattgcat tagcattgta taattccgat agtgattaag
    17221 ggaggattta ttcgtgaaac aacgaggaca aacaaatatc gtttacttgc tattactggt
    17281 aggaattata tctctactgt tatattcatt tacagggaat cgctctgcta ataatgttat
    17341 ttccatcaat caattggcta atcagattaa acaaggcaat attgccaaaa tcgaatccaa
    17401 ttcgacaacc gttaacattc tttataaaga tcgctcggtg gcaaaggccg taatcgagcc
    17461 caattcttct ttgttggaac agctgacctt attgggcgta actccggaag aactttcatc
    17521 ggacaacatt gaaatttcca tcgtcgaaga atcaagctgg gttggaattt tatccattct
    17581 cagttatttc ttgccggtca tatttttggc attggctatg ttctttattt tccggcaaac
    17641 gcaaagcggc aacaattccg ctctttcctt cggaaaatcg cgggcaaaaa tgttcgaagg
    17701 tgatcatccg acggtaactt ttgcggatgt cgccggtgtc gatgaatcca aggaggagct
    17761 tcaggaaatt gtcgaatttc tgaaggaacc gcaaaaattt actcggctgg gagcgcgaat
    17821 tccgaagggc gttttactgg tcggttcacc cgggaccgga aaaacgttgc tggcaaaagc
    17881 ggtttccggc gaagccggtg tacccttctt ttccatcagc ggctccgaat ttgttgaaat
    17941 gttcgtcggc gtcggcgcca gtcgtgtccg cgatctgttt gaccaggcaa agaaaaacag
    18001 cccttgcatc atcttcatcg atgaaatcga tgcggtaggt cgccagcgcg gagccggatt
    18061 aggcggaagc catgacgaac gggaacaaac attgaaccag atgctggtcg aaatggacgg
    18121 ttttgagacg gacaccaacg tgattatcat tgcggcgacc aaccggcctg atattttgga
    18181 tccggcattg cttcgacccg gtcgtttcga ccggcaggtg gttttggatc gcccggatgt
    18241 cgtcggacgg gaagcaattt tgaaagttca ttccaaaggg aaaccgttgg ataaagatgt
    18301 cgatctgaat gctttagcgc gcgccacacc gggatttgtc ggagccgatc ttgaaaacct
    18361 gatgaatgaa gcggcaattt tagcggctaa aagaaacaaa aacagcatcg ggaacgcgga
    18421 aatggaagaa gcggtggaac gggttatcgc cggaccgcaa aagaaaagcc gaattcttac
    18481 cgaaaaggaa aaaagaatta tcgcttacca tgaggcaggc catgcgatta tcatgaatgt
    18541 tattccggaa tgtgatccgg ttcataaggt gacaatcatc ggaagaggtt ccgccggcgg
    18601 ttacaccatg catcttccga cggaggatct ggttctgatg tcgaaaaagc agattgaagc
    18661 cgagatggtc agtctgcttg gcgggcgtgc agcggaggaa ttgatgtttg atgatattac
    18721 ttccggcgct tccagtgaca ttgaacgtgt caccgagctg gcaagaagta tggtgactcg
    18781 cttgggaatg agcgaggtga tgggaccgat cgtttacggc aaaaaagagg aaatgatctt
    18841 tttgggacga gagatctcgg aacaacggga ttattccgaa gcggttgccg aggatatcga
    18901 tgcggaagta cgtaagattg tcaacagtgc ttttgaaaaa gcaaaagcgc tgttggttga
    18961 atatcgcgaa tatttggata agattgcgaa caagttgatt gaggtggaga cattgacgaa
    19021 agaggaattc gaagcaatat ttccgcctcc gaacggaaaa gtgtccgcaa caccgaatta
    19081 tctggaacaa aagcaagcgg tttaaaatat tccggaatct gagaacaaaa aagcgccgat
    19141 caatcggcgc ttttttgttc tgcttcttgg gattttcatc ttataaaaaa accactcctt
    19201 cccc
//
LOCUS       DAOSMM010000044        18432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3034_length_18432_cov_16.661479, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000044 DAOSMM010000000
VERSION     DAOSMM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 18432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18432
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_18432_cov_16.661479"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..655
                     /gene="hpt"
                     /locus_tag="PK898_07225"
     CDS             104..655
                     /gene="hpt"
                     /locus_tag="PK898_07225"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOU19861.1"
                     /translation="MHITTEENDIAEVLFSEEAIQKRVKELAAQLTKDYEGLDPLMVC
                     VLKGASFFYADLCRQIKCRIFMDFMAVSSYENESKTTGVVRIIKDLDKNITDRHVVIV
                     DDIIDSGLTLKYLKDLLSARKPASIKTISLIEKQLGLKDRIVPDYNGFTMGDHFVVGY
                     GLDYSDYYRNLPYIGILKPETYR"
     gene            760..1920
                     /locus_tag="PK898_07230"
     CDS             760..1920
                     /locus_tag="PK898_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU19862.1"
                     /translation="MENYQPEKRKPANKPFGISKTLWITIAVILLLVLFFITFKPVYI
                     MVGLGQQEMGVKIRAGEIIDIVGPGGIYSDFGLYVKMERYNIEGVQFQVSDDEVFTKD
                     MQPVYVEIRGQVFRPALNSVKIIGSDGTVQYFSKDEITKNFVNYRQIYTDNAALETQM
                     DGFSRQAMKVCVGQNDLRGNAVAEGRDILRNCIEAELEKLTRDIGLYVNNIVVTDVKA
                     SEQAMVVINETNQYLLDAEKAKAQSQKLEEEGKANAVQKEAEVRVQQAAAQETARQKV
                     VLAQLQEQELIANRSVLEAEKQNTILEQSLNIEKAKAATELAKANLAETTLLAQIYQD
                     NPAYYQYLIAQLNASAIKATDKVMILPQGTIPQLVLGNDIVPTYPVESKPVE"
     gene            complement(1981..2502)
                     /locus_tag="PK898_07235"
     CDS             complement(1981..2502)
                     /locus_tag="PK898_07235"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOU19863.1"
                     /translation="MSYSKEVAEKAYRLAFEYEANKGSCSQCVLAAIMDTIGGIDDSV
                     FQAAQGLSGGTVSCGTGTCGALAGGITALGSRVGRTRQEFDEDIKNRRVVEIGRKLAD
                     KFIQTYGGITCREVQTANMGRSYNLADPVENQQFAAAGGHAEKCASVAGNAAKWTVEI
                     LNELEAGESASAK"
     gene            complement(2499..4070)
                     /locus_tag="PK898_07240"
     CDS             complement(2499..4070)
                     /locus_tag="PK898_07240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19864.1"
                     /translation="MTEKSYSGNLFFLLLACILILGAFIFIKTTPSGIGLVSDSVNYI
                     NGARSIAEGKGYYRESGGGTIKPITNFPPLYSILLSVPILFGAGGIQAAWAFSLIFYL
                     LNITMIAILVRAVTKNGWLGLAGSLLFCASKPFLYFQVFAMSEPLFFFCTLMAFYLLW
                     LGFQTDSWVRWLLCGVFCGAAFLTRYVGVVSLAVTVGILLFLPPARVRRMKAIGWVLL
                     GSVPLMMSWLIRNLIVSGNVTNREAYFHPLSGEEIHTGIMIFWRWIYPERYQNVTTPV
                     SWMRLGIILIGIAGVLFLAGTFLLSVRKKDISNEVRMIWAFVLYLFVYLAVIFITITF
                     LDWSVNIEERILFPCLMVMLLTLLTLIGMFLRKKKIFPIIGLLAVYGFLLITSSQDLI
                     QFIPRFGEVGYGWAWQGWEDSPAMNLIKEMPADTVIYSNQPEAVSLIAGRGAYALLDP
                     IDPSTGLKRPGYDETMATIRNQVIQGEAVLVFFGINSWVEKDQENWITRLCEGLPYIY
                     QDQSEWVVGKKEILK"
     gene            complement(4067..5296)
                     /gene="mltG"
                     /locus_tag="PK898_07245"
     CDS             complement(4067..5296)
                     /gene="mltG"
                     /locus_tag="PK898_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOU19865.1"
                     /translation="MNKKEPDEIQVSEGIQSDLTDFERLGKKKNRRSKKILGAVFVIC
                     VLFLAVYFNGVKKKVESVIGPASPALSRFQQFRLIIRLEPELSRLTGPASDKIDQTLL
                     SVQSGETPAEIARELKEKQVLSDEDLFLRYLIYSGADRQLKPGRYIIPAGSNIPEIGK
                     VLTDTGNSLVEFSILNGMRLEEIAALLPSSGLSISPEDFLQAARNYPGDLHPAGGTDL
                     EGYIPAGSYIVARSISVQNFLKGFTDTFNQKLTREVAEGLQRKGLSVAEGVTLASIVS
                     REAMSSEEYGLIASVFLNRYRLGMPFQSDPTVQYAIGWDETEKTWWKNPLASSDLLIQ
                     SPYNTYIYNGFPPTPICNFNFEVLEAVANAPETDYYYFRMRCDGSPFHNFAETYAEHV
                     ANACTKEETATPFEINQ"
     gene            complement(5289..5741)
                     /gene="ruvX"
                     /locus_tag="PK898_07250"
     CDS             complement(5289..5741)
                     /gene="ruvX"
                     /locus_tag="PK898_07250"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOU19866.1"
                     /translation="MGRILSVDPGEKNIGLAISDPLGITARSLGVIRHVGRDQDVQKI
                     VSIALENDAETILIGQAVGQNGEIGPSARHAQNLADAVRQNFRGEVILWDESGSTNAV
                     QDLYITMNVPKSKRRGHQDARAAAYILQDYLDWLAFQKQYKKEDQRDE"
     gene            complement(5726..7246)
                     /locus_tag="PK898_07255"
     CDS             complement(5726..7246)
                     /locus_tag="PK898_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HOU19867.1"
                     /translation="MMKTEKIQLQPFDNIDSILDKLNQTYQATYGLTQIQILWPRRGK
                     LLDDPLDFGRISGWAERNHVKLVFVSSNEQIMRKAAEHNIPVYPEVDSIPSAGTNRKD
                     RFPMKRSESVRAIRIAKLKSEREQLKEKKVPVSSELPLFLVAMVAVVFLFFLIVPHAV
                     ISIRPVETKREIILPLWTSDTLDAMTLNGGVPSELMRFTVEKTVTVPASGTIQSAGTL
                     STGTLRLTNVCDQRQLIPSGTHFSASTDQPWLFHNLTDIVLPAKTETTIPIEADKPGA
                     SFNLPAQSIHAIEVPYDRCIQIMQDQPTSGGSDGIFITPTQADYEAAVEKIRREILSM
                     PEEEVQEKAGNLRIPLMQTLKIGNLISEKSDPLIGYAGKTLTLTQTVEVEVRTISRQL
                     IIQQIRMNLEAGPQNAFIPINDSIQFSVSEKTDSGNEDQYQLEVRASQSGLQVVDYSE
                     LRELISGKTIQDAKKTIREHINLESEPSIKIWPKGLPRLPFVSSNILIEDQQWEEF"
     gene            7462..8781
                     /gene="hflX"
                     /locus_tag="PK898_07260"
     CDS             7462..8781
                     /gene="hflX"
                     /locus_tag="PK898_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU19868.1"
                     /translation="MMENELKLTQKPAERAFLVGAEIKNQPGLLSIADSLEELRLLSE
                     TAGLEVVGETSQKLDKPNPATYIGSGKVEEVRALAEETLADVVIFDNELSPRNLRDLS
                     KALGDSVRVIDRTALILDIFAQHAKTREGALQVELAQYEYRLPRLTHAWAHLERQGAG
                     ASGRTGSVGGVGLRGPGETQLEVDKREIRKRIAVLKKQIEKMSDHRNLYRNQRKHSQI
                     PIVSIVGYTNAGKSTLLNLMSNSDIYTADKLFATLDPTTRRVNLPGNHLSLFTDTVGF
                     IQKLPTQLIAAFHSTLEEITEADLLLHIIDISHPNALAQFESVRETLKEIGAENIPIL
                     NVFNKADLLEDAEEAKNLLAETDPNAYLISAKTGQGIDELKTGIMEQLFEKMVPIRVR
                     LPFREGALIALFHEEGIVESVENESGGVVIQGAVPGRYLTRFQKFLV"
     gene            complement(8843..9040)
                     /gene="rpmF"
                     /locus_tag="PK898_07265"
     CDS             complement(8843..9040)
                     /gene="rpmF"
                     /locus_tag="PK898_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU19869.1"
                     /translation="MTPLPKRKLSSARRNRRRANDFLTANNLTQCPNCGEMRLPHTVC
                     PSCGYYRGREVINMTKETKSE"
     gene            10392..11114
                     /locus_tag="PK898_07270"
     CDS             10392..11114
                     /locus_tag="PK898_07270"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU19870.1"
                     /translation="MEIRIGVSKTARCSSDDSADTIDIIERPAGGGYSVVLSDGSVNG
                     QDSKVVSFMTANKISALISEGVRDSTAIRATSDQLYTTYNGSAQALLYVISVDLVTDT
                     IVISRNNPYPIYCYQRGKFIDWSNDSQPIGSSKHIQPSISEIAIEAGTIIVMVTDGVF
                     KAGQEYGTEIDIPMLVQSMIDDDNATSAQEISDFILNQALLLDEAKPKNDMCVMVMRI
                     LDENSGCIRKFTYLLPIDDPLQ"
     gene            11308..12912
                     /gene="secD"
                     /locus_tag="PK898_07275"
     CDS             11308..12912
                     /gene="secD"
                     /locus_tag="PK898_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOU19871.1"
                     /translation="MKKPIVIKLVVVIVIFLCALYIDLPHQNGLRIGNFTRSGDLYLG
                     LDLQGGMQVLLEADVEPGTTVTAEQMSTARKILENRSNGLGVSDIVFQTAGENRILAE
                     FPGMTNADEVLATLKGTGLLEFVDTGDQYFPEGEAIKTDLQHSTSSETNAVNPTSSAS
                     SDTNVVNSSSSSSTESAGTAVTALQSSATESITKSVETTAQPSAESSLGNAEGTTQPT
                     AQTSENVEATPQPTAATTEQPAGEEKIYHTVLTGSALKNVTVQMYNNQPVISFELKSE
                     DAKTFADFTSQNVNKFLTIVLDGKVISSPRINEPITDGKGVISGNFTIDSANALAIQL
                     KYGALPVPLKVVEYKLIGATLGEDSLQKSLVAGLIGLSIAVLFMLIYYRLPGLIAVLE
                     IIFYGAVTLAIYKLIPVSLSLSGIAGFLLTTGGAFDANILMFERLKEELRNGKTISQA
                     VGLSWPRAWSSIRDSNIATLITAIILFYFGSSFGASVVKGFAVSLIIGVLISMFSANF
                     VTDTLLHVFTKSIADVPENKSKWFGI"
     gene            12925..13860
                     /gene="secF"
                     /locus_tag="PK898_07280"
     CDS             12925..13860
                     /gene="secF"
                     /locus_tag="PK898_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOU19872.1"
                     /translation="MKIDILSKRYWYFIFSLILIVPGLVLLAIKGMPYGIDFTGGTMM
                     ELQFSQGNRPEREAFENEIINYGIDATVVLSGENNVIIRAPYLEDSEIEKLIDYVAKQ
                     YGESPATLRYSISSVGPSIGSEVASRAALAVLAAALGVILYIWFAFRSIPNSFRYGVC
                     AVIAMIHDSLIVISLSCLGQFWGWQFDSLTLTALLTVIGFSVQDKIVVFDRIRENSRI
                     YRKLDFETLVNHSIVQTLERSINTQLMTSEFMLLAMALLGGSSLRDFSIILLVGLLMG
                     TYSSIFIAAPLLVVWQNKEWKNWFGHGNKKAVSEG"
     gene            14155..16014
                     /gene="htpG"
                     /locus_tag="PK898_07285"
     CDS             14155..16014
                     /gene="htpG"
                     /locus_tag="PK898_07285"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOU19873.1"
                     /translation="MANKQIPFKAETKQLLDILINSLYSDRDVFLRELISNASDALTR
                     MNFEMLTNSNVVDADAKPAIRIYPDKEANTLKIVDNGIGMNETEVKENLGTIAKSGAR
                     DFLEVAKDQPAGMVNDLIGQFGVGFYAAFMVAEWIEVDTRSYRPEDKAVKWRSNGTGT
                     FTISDSDKQSRGTEVLIKLKEDAKEYTDEYKLREVIRKHSNYIPFPIYIGDSEEQVNS
                     ETVLWRKNPSELKDEDYEKFYQNFTMDFEKPLAKLHLSIDAPFQMYALLFIPAKPERA
                     IFSLRKEDGLQLYARKVLIQDYCTDLLPRYYRFVEGVVDSEDIPLNVTRETMQANRVI
                     ITLRKILAGKVTDMLKKLGADQPETYTTFWKRYGPFICEGIVTEPESKESLIPLLRYR
                     TLNHNDEYRSLADYVSEMKPGQDKIYYILGEDIETARNSPHIEMLKKDGLDVLLMVDP
                     FDPFVVTELEKYQDFPLVNAATEKAEAKKDDKDQEEKTSEEPKEPSAGEYADLIKMFK
                     EELGEKVSDVRVTGRLVESPARMVNAEGALSPEMQKMYNLMQQDFQKPKKILEINPKH
                     PLIAKLEKLEDKQLANQIIDQIYDGAMLMDGEKTDQYQMLNRIQNILINLLNK"
     gene            16156..18276
                     /locus_tag="PK898_07290"
     CDS             16156..18276
                     /locus_tag="PK898_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOU19874.1"
                     /translation="MNKEVLDGLFRPKSVAVIGASGTPGKIGYSVVEALINGKYEGKI
                     YPINLKLTEILGRKAYPTIGDVPEEVDLAVITIPAKFVLGAVEECGKKGVKGISIITS
                     GFSEIGDHETENAIVETARRYGMRILGPNIIGYISNSDKLNASFAPMLPLPGNASLIS
                     QSGALLIALEAGTYLRGVGFDKLFSLGNMADIDFADVIEWLNEDENTKCISLYIEGLK
                     DGRRFMEKCKESTKPIIVLKSGVSAHGAAAAASHTGSLAGAAKVYSAAFEQSGIIQAA
                     NLDNLFDLTEAFELQPPMLGDHLLVITNGGGVGVLATDSAEKYGIPLEFCPPDLQEEM
                     KHYMPEFGSAKNPVDITGGAGLEGYQKSISFGLRQDWVDGLVVLYCETAVTNPMEIAQ
                     GIYGAIMEAGDILKTKPVTVSIIGGERSAEAIRWLIDHGIPSYDDPDRAVNAISALRQ
                     YARLQESKNHAGEEKPYKADRNAALEIINAARADGREALTEVEAKQVFQAYGLNVAAT
                     QLAKSEDEAVKLANKIGYPVVMKIVSPDILHKSDAGGVKVNIKDDHAVRDAYNTIMKN
                     AKAYKADAAIYGIAIQEMAPLGTEVILGSINDSAFGPTVMFGLGGIFVEVLQDVTFAV
                     TPVSKKRGLKMQSDIKGAKILSGARGEAPRDKDAMAETIAMYSNMILDLKDEIKESDA
                     NPVMVYEAGKGLKIVDARIILKKK"
BASE COUNT         5273 a         4126 c         4045 g         4988 t
ORIGIN      
        1 atttgtaata tccggcagga acaagtattt gaatgatata aaattaaaac aacaaacaaa
       61 acgcatgacg gttaaaaatt attaaataat aaggataaag aagatgcata tcacaaccga
      121 agaaaatgac atcgcggaag tgcttttctc tgaagaagcg attcaaaaaa gggtaaaaga
      181 attggcagct caactcacaa aggattatga aggattagat cctctgatgg tttgtgtgct
      241 gaaaggcgcc tcatttttct atgccgatct ctgtcgccag atcaaatgcc gcatatttat
      301 ggattttatg gctgtttcca gttacgaaaa tgaatcaaaa accaccggcg tcgtccgtat
      361 catcaaagat ttggacaaaa acattaccga tcgccatgtg gtcatcgtag atgacattat
      421 cgattcgggt ttgacgttga aatacttaaa agatttattg agcgcccgca agccggcttc
      481 tattaaaacg atcagcctaa tcgaaaaaca actcggttta aaagacagaa tcgttccgga
      541 ttataacgga tttacgatgg gcgatcattt tgtagtggga tacggtttgg attattcaga
      601 ctattatcgg aaccttccat atatcggcat tttgaaaccc gaaacatatc ggtagaattt
      661 tcgtatatga gttgattgcc ttaatattat tttccaccaa ccggtccgaa attagttctg
      721 tccgttttga gaccggaata taaaaacacg gagatgaaaa tggaaaatta ccagcccgaa
      781 aaaaggaaac cggcgaataa acctttcggg atcagtaaaa cgttgtggat cacgatcgcc
      841 gtgatcttgc ttcttgtttt attttttata acatttaaac ctgtttatat tatggtcggc
      901 ctcggtcagc aggaaatggg tgtcaaaatc cgagcgggtg aaattatcga tattgtcgga
      961 cccggcggta tctacagtga ttttggtttg tatgtgaaaa tggaacgcta caacatcgaa
     1021 ggtgtccagt ttcaggtatc ggatgacgag gtctttacca aagacatgca acccgtttat
     1081 gtcgaaatcc gcggtcaggt cttccgtccg gcactgaaca gtgtaaagat tatcggaagt
     1141 gacggtacag tccaatattt cagcaaggat gaaataacga aaaactttgt gaactaccgg
     1201 caaatttata ccgataacgc ggcgctcgaa actcagatgg acggattctc ccggcaagcg
     1261 atgaaggtct gtgtcggaca aaatgacttg agagggaatg ccgttgcgga agggcgggac
     1321 attttgcgca actgcatcga agctgaattg gaaaaattaa cccgagatat cggattatat
     1381 gtcaataaca ttgtcgtgac cgatgtgaag gcttccgaac aggcgatggt cgtcataaat
     1441 gagaccaatc agtatctgct tgatgccgaa aaagccaaag cccaatcgca aaaattagaa
     1501 gaggaaggaa aagccaatgc cgtccaaaag gaagcggagg taagggttca acaagccgcc
     1561 gctcaagaga ccgctcggca aaaagtcgtt cttgcacaac ttcaggaaca ggaattgatt
     1621 gccaatcgat ccgttttgga agcggaaaag caaaatacga ttttagagca gtcgctgaac
     1681 atcgaaaaag ccaaagccgc taccgaattg gcgaaagcga accttgccga gacgaccctt
     1741 ttggcacaaa tctatcagga taatccggcc tattatcagt atcttattgc tcaattaaat
     1801 gcatccgcca tcaaagctac ggataaagta atgattcttc cacagggaac catcccgcaa
     1861 ctggtcttgg gcaacgatat tgttccgaca tatccggttg aaagcaaacc ggtcgagtaa
     1921 atcttgatat tgtcaaaaag gagctgcttt tttttttacg aaaagcagct ccttttttat
     1981 ttatttcgca gaagcggatt caccggcttc taattcgttc aatatttcca cggtccattt
     2041 tgcagcattt ccggcaacgg aggcacattt ttcagcatgt ccgccggccg cggcaaattg
     2101 ctgattttca accggatcag ccaaattata ggatcttccc atgtttgcgg tttgaacctc
     2161 tctgcaggta attccgccat aggtttgtat aaatttatcc gccagtttcc ggccgatttc
     2221 cacaactctt cgattcttaa tatcttcgtc aaattcttgt cgggtccggc cgacacggct
     2281 tcccaatgcg gtgatcccgc cggcaagcgc accacaggtt cccgttccgc acgaaacggt
     2341 cccgccggac aaaccctgag ccgcttgaaa taccgaatca tcaattccgc cgattgtatc
     2401 cataatcgcg gccaggacac attgcgaaca agatcctttg ttcgcctcat attcaaatgc
     2461 caatcgatat gctttttccg ccacttcttt tgaataactc attttagaat ctcctttttt
     2521 cctacaaccc actcactttg atcttgataa atgtaaggta atccttcaca taagcgggta
     2581 atccaattct cttgatcttt ctcaacccat gaattgatcc cgaagaaaac caaaaccgct
     2641 tcaccttgaa ttacctggtt gcgaatcgtc gccatagtct catcatagcc cggccgcttg
     2701 agtcctgtgg acggatcgat cggatccagc aaagcatacg ctccgcgacc ggcaatcaaa
     2761 ctgaccgctt cgggttgatt ggaataaatt acggtatccg ctggcatctc tttgatcagg
     2821 ttcatcgccg gagaatcttc ccatccttgc catgcccaac cgtaacccac ttcgccaaag
     2881 cgcgggataa actgaattaa atcctgagaa gaagttatca acagaaatcc atacaccgcc
     2941 aataaaccga taatcgggaa gatttttttc ttcctcaaga acatcccgat taaagtcaaa
     3001 agagtcaata acatcaccat cagacaggga aataaaatcc gttcctcaat attcaccgac
     3061 caatccaaga atgtgatcgt aataaagata acggcaaggt agacaaacag ataaaggaca
     3121 aaagcccaaa tcattctcac ttcattcgaa atatcttttt ttcgaaccga taaaaggaat
     3181 gtccccgcta agaacaagac accggctatc ccgatcaaaa taattcccaa tcgcatccat
     3241 gaaacaggcg tcgtgacatt ctggtatcgc tccggatata tccatcgcca aaaaatcatg
     3301 atcccggtat gaatctcttc tccggaaagc ggatggaaat aagcctcccg gttggtaaca
     3361 ttccctgaga caatcagatt ccgaatcagc cagctcatca tcaaaggaac cgaaccgagt
     3421 agaacccatc cgatcgcttt cattcgccga acgcgtgcgg ggggaagaaa gagcagaatt
     3481 ccaaccgtca ccgccaaaga cactactcca acataacggg tcagaaaagc ggctccgcaa
     3541 aaaaccccac ataacaacca ccggacccat gaatcggtct gaaaaccgag ccatagcaga
     3601 taaaaagcca ttaatgtaca gaagaaaaac agcggctccg acatcgcaaa cacttgaaaa
     3661 tacaggaatg gcttggaggc acaaaataga agagatccgg caagtcccag ccatccgttt
     3721 ttggtcacag cccgtaccaa tattgcgatc atcgttatat ttaataagta gaaaatcagg
     3781 ctgaatgccc aggcggcttg aattccgccg gcaccgaaca ggatcggtac ggaaagcaaa
     3841 atggaatata aggggggaaa attagtaatc ggtttaatgg tccccccgcc gctctcacga
     3901 taatatcctt ttccttcggc gatcgatcga gcaccgttaa tataattgac ggaatcactg
     3961 actaacccga taccggaagg cgtagtctta ataaaaatga atgcaccgag tattaatatg
     4021 catgccaata atagaaagaa caggtttccg gaataggatt tttcagtcat tggttgatct
     4081 cgaacggtgt agcggtctct tcttttgtgc aggcattggc aacatgttct gcataggttt
     4141 ccgcaaaatt gtgaaatggt gatccgtcac agcgcatcct aaaataataa taatcggttt
     4201 cgggcgcatt tgccaccgct tccaatactt caaaattaaa attacagatc ggtgtgggag
     4261 gaaatccatt ataaatgtaa gtattatagg gcgattgaat caataaatca gagcttgcta
     4321 atggattttt ccaccaggtt ttctcagttt catcccaacc gatggcatat tgaacggtag
     4381 ggtcactttg aaaaggcatt cccaatcgat atcgattcaa gaaaaccgaa gcgatcagtc
     4441 catattcttc gcttgacatg gcttcccgtg agactatgga tgccaaggtt actccttccg
     4501 caacgcttag tcctttccgc tgaaggcctt ctgcgacctc ccgagtaagc ttttgattaa
     4561 aagtatccgt aaatcctttc aaaaaatttt gaacggagat cgaccgagcg acgatatagc
     4621 tccctgccgg aatatatccc tctaaatcgg ttcctcccgc cggatgaagg tcgccgggat
     4681 aatttctcgc agcttgcaaa aaatcttccg gagaaatcga aagccccgaa ctcggcaata
     4741 aagcagcgat ttcctcgaga cgcattccat ttaaaatcga gaactcaacc aggctgttgc
     4801 ccgtatccgt caacactttc ccaatctccg gaatattcga ccccgccgga ataatataac
     4861 ggccgggttt cagttgtcga tccgctccgc tataaatcag ataccgaaga aacaaatcct
     4921 catcggataa aacttgtttt tcttttaatt cgcgggcgat ttcagccggc gtttctcccg
     4981 attgaaccga taacaaggtc tgatcgattt tatccgaagc cggtcccgtc aaccggctta
     5041 attcgggttc caatcggatt atcagacgaa attgttgaaa tcgacttaat gccggggaag
     5101 ccggaccgat cacgctttcc actttctttt tcaccccgtt gaagtaaacc gcaagaaaca
     5161 gaacgcatat gacaaagaca gcgcccaaaa ttttcttcga tcttcggttt ttcttctttc
     5221 caagacgttc gaaatccgtc aagtcgcttt gtattccctc cgatacttga atttcgtccg
     5281 gttctttttt attcatcccg ttgatcttcc tttttatatt gcttttgaaa tgcgagccag
     5341 tctaaataat cctgaaggat ataagcggct gcccgggcat cctgatgtcc gcgacgcttt
     5401 gatttgggaa cattcattgt gatataaaga tcttgcaccg cattcgtgct tccggattca
     5461 tcccagagaa tgacttctcc gcgaaagttt tgtcgaacgg catccgccaa attctgcgca
     5521 tgccgagcgg acggaccgat ttctccattc tgccccactg cttggccgat taaaatcgtt
     5581 tcggcatcat tttccagcgc aatcgaaacg attttttgaa catcctgatc ccttccgaca
     5641 tgacgaatca caccgagcga tcgagctgtg attcccagtg gatcgctgat cgctaaaccg
     5701 atattttttt cacccggatc gacgcttaga attcttccca ttgctgatcc tctattaaga
     5761 tatttgaaga aacaaagggc aatcggggaa gccctttcgg ccatattttg atggaaggtt
     5821 ccgattcaag gttaatatgc tccctaatgg ttttttttgc gtcctgaatc gttttcccgg
     5881 aaattaattc tctcaactca ctgtaatcca caacttgcaa ccccgattga cttgcccgca
     5941 cttccaattg atattgatct tcgtttccgg aatccgtctt ttccgatacg gaaaattgaa
     6001 tggaatcatt gatcggaata aaagcatttt gcggaccggc ttcgaggttc atccgtatct
     6061 gttgaataat aagctgacga ctgattgttc gtacttcaac ttctactgtt tgcgtaagcg
     6121 tcaatgtttt tccggcatat ccaattagcg gatccgactt ttcggaaatt aaatttccga
     6181 ttttcagtgt ctgcatcaga ggaatccgca gatttcccgc tttttcttga acttcttctt
     6241 ccggcatcga taaaatttcc cggcggattt tttcgactgc cgcctcataa tccgcttggg
     6301 tcggcgtgat aaaaatgccg tcgcttccgc cacttgtcgg ttgatcctgc atgatctgta
     6361 tacatcgatc ataaggaacc tcgattgcgt gaatcgattg agccggtaaa ttgaaagaag
     6421 cacccggctt gtccgcttcg atcggtattg tggtttcggt tttcgccgga agaacaatat
     6481 cggttaaatt atgaaacaac caaggttgat cggttgaagc ggaaaaatga gtccccgacg
     6541 gaatcaattg tctttgatca caaacattgg ttaatctgag ggtaccggtt gacaatgtgc
     6601 ctgcggattg aatcgtaccg ctggcaggca cggtaaccgt tttttcaacc gtaaaacgca
     6661 ttaattccga aggaacccca ccgttcagag tcatcgcatc caatgtatca ctggtccaaa
     6721 gcggaagaat gatctctctt ttcgtttcca ccggtcggat tgagattacc gcgtgaggga
     6781 caatcagaaa aaacagaaac acaaccgcaa ccatcgctac caaaaatagc ggtaattccg
     6841 atgaaacggg tacctttttt tctttgagct gttccctttc cgatttcagt tttgcgattc
     6901 tgatcgcacg gacgctttcc gaccgtttca tcgggaaacg atctttccga tttgtaccgg
     6961 ccgacggaat cgaatcaact tccggataaa ccggaatatt atgttccgcc gctttcctca
     7021 taatttgttc attggaagac acaaagacca acttgacgtg atttcgttcc gcccagccgc
     7081 tgattcgccc gaaatccaat gggtcatcaa gtaatttgcc ccgcctcggc cataaaatct
     7141 ggatttgcgt caagccgtaa gttgcctgat acgtttggtt cagtttatcc agaatagagt
     7201 cgatattatc aaatggctgt aactgaattt tttcggtctt catcatgaaa aattatacct
     7261 gagtcgaagc tttgccgttt ttctatcctt gattctttta aaatcaatat tcgaaattct
     7321 tatccggtca ggaagaaaac gatttaatgt ttcaatgaga catttttaat catgttgcga
     7381 tgacgaatcg aaaaaaacca aggtatcggt ttcatttttg tttcacctca accgaatttt
     7441 tctcagtata atcgttgcta tatgatggaa aatgaattaa aactaacaca aaaaccggcg
     7501 gaacgggctt ttttagtcgg agccgagatt aaaaatcagc cgggtttgtt atcgattgcg
     7561 gattcattgg aagaattgcg tttgctttcc gaaacagctg ggttggaagt tgtgggggaa
     7621 accagccaaa aattggataa acccaatccc gctacctata tcggcagcgg gaaagtggaa
     7681 gaagtgcgcg ctttggccga agaaacttta gcggatgtgg ttatttttga taatgaactc
     7741 agcccgcgaa acttgagaga cctatccaaa gccttgggcg actcggttcg ggtgatcgat
     7801 cgtacggcat taattttgga tatatttgca caacatgcaa aaacccgcga aggcgcttta
     7861 caggtggaat tagcccaata tgaataccgg ctgccgcgcc tgacgcatgc ctgggcacat
     7921 ctggaaaggc agggagccgg cgcttcaggc agaaccggca gcgtcggcgg agttgggctg
     7981 cgcggccccg gtgaaacaca attggaagtc gataagcgag aaattcgtaa gcggatagcg
     8041 gttttgaaga agcagattga aaagatgtcc gatcaccgca atctgtatcg caatcagcgg
     8101 aaacattcac aaattccgat tgtatcaatt gtcggttaca caaatgccgg gaaatcgaca
     8161 ctgctgaatc tgatgtcgaa ttcggacatt tataccgctg ataagctctt tgcgacactc
     8221 gatccgacca cccgacgtgt taatcttccg ggcaaccatc tttctctctt taccgatacc
     8281 gtcggcttta tccaaaaact tccgacacaa ttgatagcgg cttttcattc caccttggaa
     8341 gaaatcaccg aagcggatct cttacttcat ataatcgaca tctctcatcc gaatgctctg
     8401 gctcaatttg aaagtgttcg ggagacattg aaagaaatcg gtgccgagaa tattcccata
     8461 ctcaatgtat ttaacaaagc ggaccttttg gaagatgcgg aagaagcgaa aaacctgctc
     8521 gccgaaactg atcccaacgc ttatctgatt tccgcaaaga ccgggcaagg tatcgatgaa
     8581 ttaaaaacag gtattatgga acaattattt gaaaaaatgg ttccgattcg tgttcgtctt
     8641 ccgttcaggg aaggggcttt gatcgcctta tttcatgagg aaggaatcgt tgaatcggtc
     8701 gaaaacgaat ccggtggggt tgtgatccaa ggggctgttc cggggcgtta tctaacccga
     8761 ttccaaaaat ttttggtata agaaattatt aaaacaaaaa cggattcttt tcggaatccg
     8821 tcgtttttca ttacgaaaaa aattattcgc tcttcgtttc ttttgtcata ttaataactt
     8881 cacgaccgcg gtagtatccg caactcggac atacggtatg tggtaaacgc atttcaccgc
     8941 aattcggaca ctgggtcaga ttattggcag tcaaaaaatc atttgcgcgg cggcgatttc
     9001 tgcgcgcact ggaaagtttt cgttttggta gcggtgtcat tttataaact cctcatttct
     9061 atccaccata aatatacccg ttcaacaagg cgaagctaat tatattcaac cagtaccact
     9121 tcgtcaactg tgaatctgct ctctgatgac agaattttaa aattggaatc agtaaaagta
     9181 aacgtatata gaattgcatt cacatctttg aagagttaag cttcaattac ccgaacgaaa
     9241 tacatatttg attacaatga accgataaac accattttat taatcaccat ccgttgggga
     9301 cggcgtcctt gcaaagcaca ccgaggaacg aggtgctgac gtcccgcgcc gagcaggcga
     9361 ggcgataatg tcccgcaatt tatcaccatc cgtagtggac ggcgtcctcg acgtcccgcg
     9421 ccgagtatac gaggcaataa tgtcccacaa tttatcattt ccgtagggga cggcatcctc
     9481 gacgtcccgc gccgagtatg cgaggcgata atgtccgagg atttatcatt tccgtagggg
     9541 acggcgtcct cgacgtcccg cgccgagtat acgaggcaat aatgtcccgc aatttatcac
     9601 catccgtagg gggcggcgtc ctcgacgtcc cgcgccgagc aggcgaggtg ataatgtccg
     9661 aggatttatt tcattatcca tatgggacat tatcccctat acattttcct atccttttgg
     9721 caaacaggac gtctacattt cgaaacttcg atgtgtttta tatgaataaa aataaacggt
     9781 ggcaatatga ttgaacactt cgtgttcaac gggatgtcgg ggacgccatc ccctacggtt
     9841 tcttttttta tggatggtga tttcaataat ttattatcct atccttttgg caaacgggat
     9901 gtctgcgcct cgtatcctcg gcgtgcttcg caaggacgcc atcccctacg gtttttattt
     9961 ttatatgagg tgatttcaat agtttattat ttcatacatt ggtaaacggg atgtctggac
    10021 ttcgtgactt cgatgtgttt cacatgaata aaaataaacg gtggcaatat gattgaacac
    10081 ttcgtgttca acgggatgtc ggcgcctcgt attctcggcg tgcttcgcaa ggacgccatc
    10141 ccctacggtt tccattttta tggatggtga tttcaataat ttattatcct atccttttgg
    10201 caaatgggat acctgcgcct cgtaacctcg gcgtgcttcg caaggacgcc atcccctaca
    10261 gtttttatga ccggcaaatc tgtaccacag atatgccggt cataaactgc cttgtcaaca
    10321 gaaccttttt ctcaagatta agatttatcg gaactatgcc gaggtttgtt ctttatgata
    10381 aaatttgagc aatggaaatt agaattggtg tttcgaaaac ggcaagatgc agctccgatg
    10441 acagtgcgga tacgatcgat attatcgaac gaccggccgg cggaggatat tccgtcgtgc
    10501 ttagtgacgg aagcgtaaac ggacaggact ccaaagtcgt ctcgttcatg accgccaaca
    10561 aaatatctgc cttaatttcc gaaggtgttc gcgacagcac tgcaatccgt gccacttccg
    10621 accaacttta cacgacatat aacggatcgg cacaggcact tttatacgtt atctccgtag
    10681 atctggtgac ggatacaatc gttatcagcc gaaataaccc ttatccgatc tattgctatc
    10741 aacggggcaa atttattgat tggagcaatg acagtcagcc gatcggaagt tcgaaacata
    10801 tccagccttc catttccgaa atcgcaatcg aagccggtac gattatcgtc atggttaccg
    10861 acggagtatt caaagcggga caggaatatg ggaccgaaat agatatcccg atgctggttc
    10921 aatcaatgat cgatgatgat aatgcgacca gtgctcagga aatttcggat tttattctca
    10981 atcaagccct tcttttggat gaagcgaaac caaaaaatga tatgtgcgta atggtcatgc
    11041 gaattctcga tgaaaacagc ggttgtatcc gaaaatttac ataccttctt ccgatcgacg
    11101 atcccttaca atagattttc aaccgaaatc cggtcttcca atcgggctat ctctttctct
    11161 ccttaactta tatcattcgg tattctgaca aaaagccctc caacattcag cgtggtattt
    11221 tccggcgttt atagattaat acgttataat taggctttgg attttcgcca aaacagcaag
    11281 tgcaccattg aggaggcaga cagcagtatg aaaaaaccca ttgtaattaa gttagttgtc
    11341 gtaatcgtca ttttcctttg tgctctatat attgacttac ctcatcaaaa cggactgaga
    11401 atcggaaatt ttacgcgttc gggtgattta tatttagggt tggatctcca agggggtatg
    11461 caggtccttt tggaagctga cgtcgaaccg ggaacgacag ttaccgcgga acagatgtcc
    11521 acagcccgaa aaattcttga gaatcgaagt aatggtctcg gagtgtccga tatcgtcttt
    11581 caaacagccg gtgaaaaccg aattctcgct gaattccccg gtatgaccaa tgcggatgaa
    11641 gttctcgcta ccttaaaagg taccggtttg ttggaatttg tcgatacagg tgatcagtac
    11701 tttccggaag gagaggcaat caagacagat ttacaacact cgacttcgtc agagacgaat
    11761 gctgtaaatc ccaccagttc ggcttcatca gacacaaatg ttgtaaattc ttccagctcg
    11821 agttcaacag aatcagccgg aaccgcggtt accgctttac aatcatccgc tacggaatcg
    11881 atcacgaaaa gtgtcgaaac taccgctcag ccatctgccg aatcctcatt gggtaatgcg
    11941 gaaggaacaa ctcagccgac tgcgcaaacc tcagagaacg ttgaggctac tccacaaccg
    12001 accgctgcaa ctaccgaaca acctgccgga gaagaaaaaa tttaccatac tgttctgacc
    12061 ggctccgctt taaaaaatgt aaccgtccag atgtataaca accaaccggt aatctctttc
    12121 gagctgaaaa gcgaagatgc caagacgttt gcggatttca cctctcaaaa tgtcaataaa
    12181 ttcctgacga tcgtattgga tggaaaagtt atttccagcc cgcgcatcaa tgaaccgatt
    12241 acagacggga aaggtgtaat ttcagggaac tttaccattg attccgccaa tgcacttgcc
    12301 attcaattga aatacggcgc tctgccggtt cctttgaaag tcgtcgaata taaattgatc
    12361 ggtgcgacgc tgggcgaaga cagcttgcaa aaaagcttgg tagccggact gatcggctta
    12421 tcgattgccg ttttatttat gctgatttac tatcgattgc cgggtttgat cgcggtatta
    12481 gaaattatct tctacggtgc cgtgaccttg gcgatttata aactgatccc cgtctctctc
    12541 tccttatccg gaattgccgg tttcttgctg acgaccggcg gtgcgtttga tgcgaatatc
    12601 cttatgttcg aacgattgaa ggaagagttg cgaaacggaa aaaccatttc gcaagcggtc
    12661 ggcttgagtt ggccgcgagc ttggtcatcg attcgtgatt caaacattgc aactttaatt
    12721 acggctatta tccttttcta cttcggatct tctttcggag cttctgtagt caaagggttc
    12781 gctgtttctc ttattatcgg tgttctgatc agtatgttct ccgctaattt tgtgacagat
    12841 acgctcttgc acgtctttac aaaatcaatc gccgacgtac ctgaaaacaa gtctaaatgg
    12901 tttgggatct aaggagggaa aatcatgaaa attgatattc tgtcaaagcg gtattggtat
    12961 tttatatttt ctttaattct aattgttccc ggacttgtcc ttttagccat caagggtatg
    13021 ccgtatggga ttgatttcac cggcggaaca atgatggaat tacagttttc gcaggggaac
    13081 cggccggaac gggaagcttt cgaaaacgaa attatcaatt acggaattga tgcgaccgtc
    13141 gtcttatccg gcgaaaataa tgtgattatt cgagcacctt atcttgaaga tagtgaaatc
    13201 gaaaaactca tcgattatgt cgcgaaacaa tacggtgaat ccccggcgac attgcgttac
    13261 agcatcagca gtgtcggacc ttccatcggc agtgaggtgg caagtcgtgc agcgctggct
    13321 gttctggcgg cggcattggg cgttatcctt tatatttggt tcgcttttcg ttcgattccg
    13381 aacagtttcc gttatggcgt ttgcgcagtt atagcgatga ttcacgactc attaattgtg
    13441 atcagcctct cctgcttggg tcaattttgg ggttggcagt tcgattcctt aactctgaca
    13501 gctttattga cggtgatcgg tttctcagtc caagataaaa tcgtggtctt tgaccggatt
    13561 cgagaaaatt cccgcatcta tcgaaaattg gattttgaaa cgttggttaa ccattccatc
    13621 gttcaaacct tggaacgttc cattaacacc caactgatga cctctgaatt tatgctcctt
    13681 gctatggcac tcttgggagg ttcttcgctg cgagatttct ccatcatctt attggtcggc
    13741 ttgttgatgg gtacctactc ttccattttc attgccgcac cgttgctcgt cgtttggcaa
    13801 aacaaagaat ggaaaaactg gtttgggcac ggcaataaaa aagccgtgag cgaaggataa
    13861 atttttttcg atcggaaaca ataaaaaaag tctcgacaaa tcgaggcttt ttttattgtt
    13921 tgaattaaca ggtcgcggcc agaatccgtc acggatgctc ataaacaaga ccaagtttta
    13981 taactgcttt catctcatct tgcagataaa aagcgttcca aagatttcac gactgaattt
    14041 tcgtctttta ttcgatttct aataaatttc atattcatga cttttataat tctgataaca
    14101 gattgaatag aaatccttat ggatctatca aataacaagc gacaggggta acttatggca
    14161 aataaacaga ttccttttaa agcagaaacg aaacaacttc ttgatatttt gatcaattca
    14221 ctttattccg acagagatgt tttcctgcgc gaattgatct ccaatgcatc ggacgctctg
    14281 acgcgtatga atttcgaaat gcttaccaat tcaaatgttg ttgatgcgga cgcaaagccg
    14341 gccatccgaa tttatccgga taaagaagcc aataccttga aaatcgttga taacggtatc
    14401 gggatgaatg aaacggaagt aaaagaaaat ctcggcacca tagcgaaatc cggcgcgcgt
    14461 gattttttgg aagttgccaa agatcaaccg gccgggatgg ttaatgattt gatcggtcaa
    14521 ttcggcgtcg gattttatgc cgcatttatg gtagcggaat ggatcgaagt ggatacccgt
    14581 tcgtatcgac ccgaggataa agcggttaaa tggcgctcaa acgggacagg tactttcaca
    14641 atttccgatt ccgataaaca gtcccgaggt accgaagtct tgatcaaact aaaagaagac
    14701 gcaaaggaat atacggatga atataaactt cgcgaagtga tccgcaaaca cagcaactat
    14761 attccgttcc ccatctatat cggggattcg gaagaacaag ttaactccga aaccgtcctt
    14821 tggcgaaaaa atccatcgga attaaaagat gaagattatg aaaagttcta tcaaaacttt
    14881 accatggatt ttgaaaagcc gctggctaaa cttcatcttt cgattgatgc accttttcaa
    14941 atgtatgccc tcctcttcat accggcaaag ccggagcgag cgattttttc cttacgaaaa
    15001 gaagacggcc ttcagcttta tgcccggaag gttttaatcc aagattactg tacagattta
    15061 ttgcctcgct attatcgatt tgttgaaggc gttgtcgatt cagaagatat tccgttaaac
    15121 gtcactcgcg agaccatgca agcgaaccga gtcattataa ctctcagaaa gattctcgcg
    15181 ggaaaagtta cagacatgct gaaaaaactg ggggcagatc aaccggaaac ttacacaaca
    15241 ttttggaaac ggtacgggcc ctttatctgt gaaggaattg ttaccgaacc ggagtcgaaa
    15301 gaaagtctca tcccattgtt gcgttaccgc accttgaacc ataacgacga atatcgctct
    15361 cttgcagact atgtgtcgga aatgaagccg gggcaggata aaatctacta tattctcggt
    15421 gaagatatcg aaacggcaag gaacagccct catattgaaa tgctcaaaaa agatggcttg
    15481 gatgtacttt taatggtgga tccctttgat ccgtttgttg ttactgagtt ggaaaaatat
    15541 caggactttc cgctggtaaa tgccgccaca gagaaagcgg aggcgaaaaa agatgacaaa
    15601 gaccaagaag aaaaaacatc ggaagaacct aaggaacctt ccgccggcga atatgcggat
    15661 ttaatcaaga tgttcaagga ggagctcggc gagaaagtct cagatgtgcg agttaccggc
    15721 agacttgtgg agtccccggc tcggatggtc aacgccgagg gtgcactttc tccggaaatg
    15781 caaaaaatgt ataacctgat gcagcaggat ttccaaaaac cgaaaaaaat acttgaaatt
    15841 aatccgaaac atcctttgat agccaagttg gagaaattgg aagataaaca actcgctaat
    15901 caaattattg accaaattta tgacggagcg atgttgatgg acggtgaaaa aacagatcaa
    15961 tatcaaatgt taaacaggat tcagaacatt ctgataaatt tattgaataa atagaatcga
    16021 ttgataaaaa aggagaaaga attttctcct tttttgtttt taatcttata aatgtgataa
    16081 aataattcag aaaatctgaa attatggcga attttcataa taatctaatt tttttaaatt
    16141 caagcgaggt aaaaagtgaa taaagaagtg ttggacggcc tcttcagacc aaaaagtgtt
    16201 gctgtcatag gggcttccgg aactcccgga aaaattggat attccgttgt cgaagcctta
    16261 attaatggta agtatgaagg caagatttat ccgatcaatc taaaattgac tgaaattctc
    16321 ggacggaaag catatccgac aatcggcgat gttccggaag aagttgatct tgctgttatc
    16381 accattccgg ccaaatttgt tttgggagcg gttgaagaat gcggtaaaaa aggtgtaaaa
    16441 gggatcagta tcatcacttc aggtttcagt gaaatcggcg atcatgagac ggaaaatgcc
    16501 atcgtagaaa ctgcccgtcg atatggaatg cgaattttgg gtccgaatat tatcggctat
    16561 atttctaatt cggataaact gaacgcttct tttgctccga tgttaccact gccggggaac
    16621 gcttcattaa tttcccaatc gggagcgctt ttaattgctt tggaagccgg gacatattta
    16681 cggggagtcg gctttgataa attattctcc ctcggaaata tggcggatat tgatttcgcg
    16741 gatgtgatcg aatggttgaa tgaggatgaa aacacgaaat gcatttctct ctatatcgag
    16801 ggattaaaag acggccgtcg attcatggaa aaatgcaaag aatccacaaa accgatcatc
    16861 gttttgaaat ccggcgtttc cgcacatggt gccgcagccg cagcctctca tacggggtcg
    16921 cttgccggcg ccgcaaaagt ctacagtgcg gcatttgaac aatcgggcat tattcaggct
    16981 gccaatttgg ataatctgtt tgacctcaca gaagcttttg aattgcaacc ccccatgctc
    17041 ggtgatcacc tgttagttat caccaacggc ggcggtgtgg gcgttttagc taccgattcc
    17101 gctgaaaaat atggtattcc gcttgaattt tgtccgccag atcttcagga agaaatgaaa
    17161 cattatatgc ccgaattcgg atcagccaag aatccggtcg atattacggg cggagccggt
    17221 ttggaaggct accagaagag catttctttc ggtttaagac aggattgggt cgacggactc
    17281 gttgtcctgt attgcgaaac agccgtaacc aatccgatgg aaatcgccca gggaatttac
    17341 ggtgcgatta tggaagccgg cgatatctta aagaccaaac cggtcaccgt ttctataatc
    17401 ggcggagaac gcagtgccga agcgattcga tggctgatcg atcacggtat tccatcctat
    17461 gatgacccgg accgcgcagt gaatgccatc agcgctctga ggcagtatgc gagattgcag
    17521 gaatccaaga accatgccgg tgaagaaaaa ccgtataaag ccgatcggaa tgccgcgctt
    17581 gaaattatta atgccgcacg tgcggacgga cgggaagcgt taacagaagt tgaagccaaa
    17641 caggtattcc aggcttacgg cttaaatgtc gctgctacgc aattggcaaa atctgaagat
    17701 gaagcggtta agctcgcgaa taaaatcggt tatccggttg tgatgaaaat cgtttccccg
    17761 gatattcttc ataaatccga tgccggcggt gtcaaggtaa atatcaaaga cgatcatgcg
    17821 gtccgagatg cctataacac gattatgaaa aatgcaaaag cctataaagc cgatgcagct
    17881 atttacggaa tcgccattca ggaaatggca cctctcggta ccgaggttat tctcggctca
    17941 atcaatgata gcgctttcgg tcccacggtc atgtttggac tgggaggaat tttcgttgaa
    18001 gtcttacaag atgtcacttt tgccgtcact cctgtatcga agaagcgggg attgaaaatg
    18061 caatcggata tcaaaggcgc aaaaatcctt tccggtgctc gcggagaagc gccgcgggat
    18121 aaagacgcaa tggctgagac gatagccatg tactcaaata tgattctgga tttgaaagat
    18181 gaaatcaaag aatccgatgc gaatccggta atggtatatg aagcaggaaa agggctcaaa
    18241 atcgttgatg cccggattat cctcaagaaa aaataagagt tccggctttt ccgaattctc
    18301 tttgacaact ttcaaaacaa caacggttgt caggtaaaaa aattaataaa agaagcgagt
    18361 gccccaaaaa cgctcgcttt ttttatctct tttctgtttc gaaaatttct tctcaacttg
    18421 aaattgtttt tc
//
LOCUS       DAOSMM010000045        18375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3040_length_18375_cov_17.934061, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000045 DAOSMM010000000
VERSION     DAOSMM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 18375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18375
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3040_length_18375_cov_17.934061"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1205)
                     /locus_tag="PK898_07295"
     CDS             complement(96..1205)
                     /locus_tag="PK898_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU19875.1"
                     /translation="MNQQAEAFIQQKRLVETFLALVRVDSPSGHEAKLSDALVKELIK
                     LDGDPHQDEIGNIVAYFNGNGSDDTVMLCAHMDTVGNDVGIQPIVLSDRITSDGTTIL
                     GADDKSGIAVILEILKVFKEHPEISHPALEVVLTVQEEVGLVGSSYLDKSQLKAKWGL
                     VLDSGGRIGGITVSGPTSRKYTFTILGKAAHAAVAPEQGINAIQAAALGITKCPCGKV
                     DPEVVIGIGVIQGGIATNIVPDKVEVKAMIRSRNPEKLERFSEQMENSFREGCEPFGA
                     KSEAEKSDSYQGYLLDENSVPWQKTKKAAELLGFPFFTEHSLGGTDCNHFNQAGIPCC
                     SVSTGMANEHMKDEYIRIQDLYDAARHVITAITIA"
     gene            complement(1237..2154)
                     /gene="trxB"
                     /locus_tag="PK898_07300"
     CDS             complement(1237..2154)
                     /gene="trxB"
                     /locus_tag="PK898_07300"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOU19876.1"
                     /translation="MKEKVPCKVLILGSGPAGISAAIYTARARLETVVITGPSLGGRI
                     SLASEIDNYPGVTDNPSGTELMSRFISQAEKFGAVLDYDTAIKVNLSGRPFEIETEGK
                     IYLAENLIITTGSRAVTMSVPGEKELTGSGVSYCATCDGFFFKDKKVAVVGGNNEAVE
                     EAVFLTKFASSVTIIHRQGELRADPIRLEKAKENPKIQYLMNSTVTAVLGKDHVEGLR
                     VKNMLTGEEQDMPFDGVFVFIGQKPISELYEGQLEMEKGLIKVDDRMRTKIPGVYAAG
                     ESVDGFYKQVVVSAGMGAMAGISLIKDLA"
     gene            complement(2151..2777)
                     /locus_tag="PK898_07305"
     CDS             complement(2151..2777)
                     /locus_tag="PK898_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19877.1"
                     /translation="MKKKRTLPAVLLIYFLLGIFALSGCGSQPKLSAEETVRAFIDAV
                     RAGDIEKASSYLDDPSVSLTVPQNRLGEAIRQSMKVVSVKENKNDETSSLFTVDVTLQ
                     MLDVLQVMSRATMLYLMDTKTDPQKIDPETIQDDQRMAAIYDTILKEDEKNPLPVKET
                     LCIFTLREQNGILKIVMDESLANTLEGNIRENLETIRKILRNQQETEQ"
     gene            complement(2774..4348)
                     /locus_tag="PK898_07310"
     CDS             complement(2774..4348)
                     /locus_tag="PK898_07310"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU19878.1"
                     /translation="MKCSINKISAFLWILLLSITVKPVSADDTGLWLDAAFPKSAVET
                     LGWQGKFVSNKVNADCALSLSVAGAPSSPENSQKIRLVYVLVAPYPTSRDVVSLKMIE
                     SFWTGKAIPSLSQILVDAETDAVFSAVWGEPNRDQVITLPADEILETAWSREKTWAII
                     PFDKSDPRWKVLKVNNISPLDFDFDPETYPLTVTWQTESLKADKNCQIPQPAGNFDPN
                     KLTTLTLTGTTAMVRRLAYAIEDQGADFITRNIGPVLSRSDFTHISNEVSYYEDCPPG
                     IPLRREARFCSLPSYSAVLKNVGADLIELTGNHIFDWGIEPFLFTLEQYREDNLKYYG
                     GGKNAGEAQQPVFISHHGNRIAVVGCNSVGPESVFATATQPGAARCDMDRLSAQVKEL
                     KEEGWMVVVTFQNFEYDDYTVPPVESHDFYAIAEAGPVIVSGSQAHVPQGFAFIGKTF
                     VHFGLGNLLFDQISDIERDSCFDRHYFYDGRYIGNVTETIRQEETMQPRFLRSSERAP
                     FLEKLFGVSDWTKVFK"
     gene            complement(4448..5857)
                     /locus_tag="PK898_07315"
     CDS             complement(4448..5857)
                     /locus_tag="PK898_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOU19879.1"
                     /translation="MRKQAFSVHGHFYQPPRENPFTGKIPQEPGATPFHNWNEKILAQ
                     CYRPNAEDGNFGRISFDLGPTLATWMDENAPDVMKEIVRQERSFYEANGVGNGMALSY
                     NHTILPLATLDDKITQIKWGIADFEARFGHQPLGLWAPETAIDLETLMVMEQCGIRYT
                     ILAPWQANTRVGLDTSRPYWVDLPQEKRIAVFFYEEQTSMRVSFDPEATVNADRFMDQ
                     YLLPLYPDNSRRERMYLIASDGELYGHHQPLRNKFLQWLTTGALVNRDIELTVPSIWL
                     RDYPPEKSIRIRNNTSWSCHHGISRWASECACAEHGEWKAPIRRAFDRIANIVDTEMQ
                     KALDPYHLKLLEFRNEYSAVLTHRETAEAQLIRLTGKTAWEEAEKNKMLNFMNAQYER
                     QRMYTSCGWFFGDFNRLESQNNILYAAKAIDYTEKATGLEFRRSISRYLSKIESHDTG
                     LKASTIFNNAFKSCNPADS"
     gene            complement(6003..6899)
                     /locus_tag="PK898_07320"
     CDS             complement(6003..6899)
                     /locus_tag="PK898_07320"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU19880.1"
                     /translation="MILVTGGTGFIGQALTKNLVSLGYPVRLLINPSGKSPRIPRGIA
                     VDVAISGIDDERNLRAAMKDVQIVFHLIGTEWKGIDAEYSTVDIPAAKTISAVAAQMG
                     VERFFYLSHLGADKASAYPVLQAKALCEAAVIASGVPYTILETAPVYGKGDHFTESFA
                     RLVRKVPFAVPLPGSGETKIQPIWIDDLITCLLLCLDDSKSIHRTFQIGGMEILSIRE
                     CLELIMEQIQVKKGFLNIPPVWLRGLLIAMEQWFEKAPKIYFWSDYLAEDRITALDVL
                     PREFSLIPTRMSRNLGYLTEAS"
     gene            complement(6896..10477)
                     /locus_tag="PK898_07325"
     CDS             complement(6896..10477)
                     /locus_tag="PK898_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU19881.1"
                     /translation="MEFHISSQSRIRYQFDSGLFRYDGNVIFADFQAVREFTQKINDV
                     RLKTGNHEPVYAGLVNSLGLIDEIMHYLIDLYRENYVPELNQQIYRWLEEKIGKEALN
                     ETLKAFCEEFPPLDVYQGKITVEEYLDEVNPKGKLNRYEAIEEMLLLWITNQNPAAMK
                     PLGELFDDKELENSTAYNDLISEIQGCFQMLPKVDGMTLVDFLLEPIKQHPDSLFDQL
                     EFIRSRWGTHLGIFLRRLLMSLDWMKEESKAQGVGPGPVEIPVYNDGSLEAGEYEAFS
                     EDRDWMPRVVLIAKNTYVWLDQLSKQYHRPITKLNEIPEEELSTLRDQGFTGLWLIGV
                     WERSKASAKIKQYCGNPEALASAYSIADYRIADDLGGENGFRELKNKAWRYGIRLASD
                     MVPNHMGIDSEWVLYHPDRFLSLPYSPFPSYTFNGPNYSPDPNIGIYIEDHYYTREDA
                     AVVFQRVDYPTGDVRYIYHGNDGTSMPWNDTAQLNYLNPEVRETVIQTILQVAKRFPI
                     IRFDAAMTLAKKHYQRLWFPEPGTGGAIPTRAEFSMTKADFDKAFPMEFWREVVDRVA
                     AEAPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNLLRDEDNAKYRILLKSTLEFDP
                     DILKRYVNFMNNPDEKTAVEQFGKGDKYFGICTMMVTMPGLPMFGHGQIQGFKEKYGM
                     EYRKAYWDEYVDTELLHRHEREIFPLLHQRRRFAEVTNFVFYNFATAYGNVDENVFAY
                     SNYGEGHHSLVVYNNRFGDTKGYIMQSVPFVVKQSDGEKYTTQKMLSESLHLHNDPEC
                     FVVFHDSASGMNYVRPSQQIWREGLYLELYAYQYYVFNDVYEVRDDSEGTWRKLNSWL
                     NGSGTLDLNDTIQELLYHYVLDPYRNCFNGRKLRWLYGKIGLAMTESEWLEIQNGTWP
                     DFEKLMDAFNIFYDLNRSKEGLFNVYCYGINEFLTDPERKMIPGMTRYNAALSKIKKD
                     ITDFPELWIALFISLQQVLLARFERGEVGSGKVENILDDWHLYKAQERILTEAGFTDH
                     LSVSEQINLIQWLYTTLADFQKWDLKTLDDRLNEMLKSDFVRHVLRTNTYQEVLWFNK
                     EAAETFSVLLELIGFYGNVFDPAASASFRIENLMVMQKAAEIFTERVRSSEYHYDRLF
                     VEIPAIENPNRTAPSQEKPKTDPKSAGSPDPADKSEQSDNASDYLKG"
     gene            complement(10526..11701)
                     /locus_tag="PK898_07330"
     CDS             complement(10526..11701)
                     /locus_tag="PK898_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOU19882.1"
                     /translation="MREVAILEIGQTPVSEHWDKSLLELAGEPILQIFEKTGISTVDS
                     IFVGNMMTGSANHQLHLGAYIADWVGAGNASAVHVESACSSGGSAFRAALMAVSSGEA
                     DTAIAVGVEKMTDSPSAEITAELATAADADYERDMGVSFVALNALLMRRYMEVYGWKK
                     EDFAGFSINAHANGAHNPNARFGFPITPEAYMKSPPVCDPINVMDAAGMSDGAAAVLL
                     VPVEKLIKGKKFVTIAGSAAATDTIALQDRRDMLWLSAAERSAKEAYRQAGIGPADID
                     LFEAHDAFTIIEALSLEASGFAERGQGPSLADPAIIGPKGKIPLATMGGLKSRGHPVG
                     ATGIYQIVEAVLQLYGLCGENQVPGAKIAMTQNIGGSGSNVYTHILKINEPRTFQNN"
     gene            11982..12356
                     /locus_tag="PK898_07335"
     CDS             11982..12356
                     /locus_tag="PK898_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU19883.1"
                     /translation="MVQTNKIELGKIGESIALETAKRRGYQMVATNVRTPYGEIDLIL
                     KTTHQLIFAEVKTRTSFSFGYPEQAITTLKLSHMIQSAQFYMLQNNLRDEYRIDVISI
                     LYDSNKHQVANLEWIENVTASE"
     gene            12337..13224
                     /gene="miaA"
                     /locus_tag="PK898_07340"
     CDS             12337..13224
                     /gene="miaA"
                     /locus_tag="PK898_07340"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU19884.1"
                     /translation="MSQHPSKIPVTVIVGPTAVGKTAFAIELAKIANAEIVSADSRYF
                     YRGMDIGTAKPTPEERQSIPHHLIDVADPDETWSLALFQQKAYDAIASIHERGKNVIV
                     VGGTGQYIRALLQGWSPPTMEADLVMRAVLEDWANEIGPMEIHKKLAILDPIAAEKID
                     YRNVRRTIRALEVIFGSGQRFSEQRKVTESPYSFLILGLNRSRSSLYQRIDQRIDQMI
                     RQGFIEEVAGLLEKGYSPALPSMSAIGYREISAYLNHEISLDEAIRLIKQRTRNFVRR
                     QANWFKTNDPQIHWFNLEG"
     gene            13282..14115
                     /locus_tag="PK898_07345"
     CDS             13282..14115
                     /locus_tag="PK898_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19885.1"
                     /translation="MQENLSQKPIYDSAKLTNPFFEEIKGLKENSFILRRMIYTNFKT
                     RYKRSYLGVVWSMLNPLLNMIVFVIIFSNLFRFDVPHYPLYILSGNMIFTFFSQSTSE
                     AMMQIIANAGMLRRVYLPKTVYILAGIGINGINLILTFIPFILIALIDRLALSWTVIL
                     IIPVLFLLSLFVVGISLVVATITPFLNDFSQIWSVIITLWMYLTPLFYPVNIIPEHLI
                     GIYQLNPMYIYVTLFRDPLLYAKIPELSLWLGGLIYGVIIFAFGWWLFTKNSNEFAYR
                     A"
     gene            14124..14858
                     /locus_tag="PK898_07350"
     CDS             14124..14858
                     /locus_tag="PK898_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19886.1"
                     /translation="MDQNIAIQTEDLGICFKMPSEPISGTKELFIKIAKKRIKINTFW
                     ALRHVNLSLNEGESIGIMGKNGAGKSTLLKAIARVLTPKEGRVYVRGKVFPMLELGAG
                     FNMELTGHENIYLYGNILGLTNEKVTQLYDSIVEFSELREFIHSPLRSYSTGMVARLA
                     FATATAVQPDILLADEILSVGDKDFQDKCMDRMRSYLNNGTTIVFVSHSPNSVKQICK
                     KGLWLEHGQVRMTGSAEEVADAYAAS"
     gene            14886..15431
                     /gene="rfbC"
                     /locus_tag="PK898_07355"
     CDS             14886..15431
                     /gene="rfbC"
                     /locus_tag="PK898_07355"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOU19887.1"
                     /translation="MDFIPLSIPDVILIKPDVHRDERGFFFESWQKNKFAKAGIDQEF
                     VQDNHSRSSKDVLRGLHYQIRQPQGKLIRAIVGEVFDVAVDLRRSSPSFGKWVSANLS
                     SENFNMLWIPPGFAHGFLVLSDVAEFVYKATDFYAPQYERCILWNDRDLGIDWPISGH
                     EPILSEKDSKGKLFREAEVYL"
     gene            15428..16501
                     /gene="rfbB"
                     /locus_tag="PK898_07360"
     CDS             15428..16501
                     /gene="rfbB"
                     /locus_tag="PK898_07360"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOU19888.1"
                     /translation="MKNVLVTGGAGFIGSNFVRYLLQREPDVKVVTLDLLTYAGKTEN
                     LEDLPHPENHVFVQGDICDRELVDQLLAEHAIDTIVHFAAESHVDRSILGPMPFIQTN
                     IIGTFTLLEAAKNAWKDKPGEFRFHHISTDEVFGALHSGDPAFEEGTPYNPHSPYSAS
                     KASSDHLVRAYYSTYNLPITISNCSNNYGPYQFPEKLIPLMILNGITGKPLPVYGKGE
                     QIRDWLHVMDHCEAIYLILKKGKIGETYCIGGDNQPTNLEIVQLICEILDDKVPRADG
                     KSHTSQIQYVSDRPGHDFRYDINTNKIQRELGWKPAHALRTGLEETIEWYLNHSAWVD
                     SVLDKDEFSSWINTNYQERDSSF"
     gene            16565..17431
                     /gene="rfbA"
                     /locus_tag="PK898_07365"
     CDS             16565..17431
                     /gene="rfbA"
                     /locus_tag="PK898_07365"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOU19889.1"
                     /translation="MKGIILAGGKGTRLYPLTRVVSKQLLPVFDKPMIYYPLSMLMFA
                     GLKEVLVISTPEDLPLYRRLLSDGSQWGMKFEYAEQPKPEGLAQAFLIGEGFLNGDSA
                     CLVLGDNIFYGSSIPTKLRTAAALENGAVIFAYEVNDPERYGVVTFDDTGKVISLEEK
                     PAQPKSNYAVPGIYFYDQEVTNYARNLKPSPRGELEITDLNRIYMEQGLLSVQVLGRG
                     VAWLDAGTHESLIQAGLYVQAVESRQGLQISCPEEIAFRMGFIDAKQLLKLAEDAGDA
                     SLKKYLRRIARE"
     gene            17516..>18375
                     /gene="rfbD"
                     /locus_tag="PK898_07370"
     CDS             17516..>18375
                     /gene="rfbD"
                     /locus_tag="PK898_07370"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOU19890.1"
                     /translation="MKILLIGEKGQLAWELARTLMPIGDIVPVDYPAFDISNKEQILE
                     TVYSVVPDLVINASAYTDVDRAETEKEKAMAINAEGPGWLAEICRSKNIPVIHYSTDF
                     VFDGMKKSPYVETDIPNPINFYGRTKLKGEEAVLNATESALIFRLAWLYSSRGNNFMK
                     KFLEWAGKYEIIKVVDDQVGNPTWARFVAEATASILAESLSSDLPLYNWIKEHKGIYH
                     LTGSGYTNRYEWSCKIWEFAPNRENLKIKRVERAKTVDFPTPAGRPAYSALDCSLVKR
                     TFSINIPSWE"
BASE COUNT         5082 a         4271 c         3924 g         5098 t
ORIGIN      
        1 cgaaaagcag gcccatatga tcatcaccgg taaagaaaag gtccggcaca actcaaaagc
       61 gatgccggac cgataaaatt ccgaacgaac tctttttatg cgatcgttat cgcggtaatc
      121 acatgccggg ctgcgtcata taaatcctga atacggatat attcatcctt catatgctca
      181 ttggccatgc cggtggaaac ggaacaacac gggataccgg cttgattgaa atgattgcaa
      241 tccgttccac ccaacgaatg ttcggtaaaa aacggaaaac ccaataattc cgcagccttt
      301 ttggtttttt gccaagggac ggaattttca tccagcagat acccctgata agaatcgctc
      361 ttttccgctt ccgatttggc tccgaagggc tcacatccct cccgaaaaga attttccatc
      421 tgttcgctga atcgttccag tttttccgga ttccggctgc gaatcatcgc ttttacttcg
      481 actttatccg gaacgatatt ggtggcgatg cccccttgaa tgacgccgat cccgatcacg
      541 acttcggggt cgacctttcc gcacgggcat ttggtaatcc ccaacgcggc tgcctgaatg
      601 gcattaatac cttgttccgg cgccacggcg gcatgagcgg ctttgccgag aatcgtaaac
      661 gtatattttc gcgaggtcgg accggacacg gtgattcccc cgattcttcc accggaatcc
      721 aaaaccagtc cccatttcgc tttcagttgc gatttgtcca gataggacga tccaaccaac
      781 ccgacttctt cctgaacggt cagtaccact tccagcgcgg ggtggctgat ttccggatgt
      841 tctttaaaaa ccttcagaat ttccagaatg acggcaattc cggatttatc gtcggcacct
      901 aaaatggtcg tcccgtcgga tgtgatccga tccgataata cgatgggctg aattcccacg
      961 tcgtttccga ccgtgtccat atgggcgcaa agcataacgg tatcatcgct gccgttccca
     1021 ttgaaatagg cgacgatgtt gccgatttca tcctgatgcg ggtctccgtc cagtttgatc
     1081 aattctttca ccagcgcgtc actcaatttc gcttcatgac cggaggggct gtccacccgt
     1141 acaagcgcta aaaaagtttc aaccaatcgc ttctgctgaa taaaagcttc cgcttgttga
     1201 ttcatgatct ggttcctttc cgtcttccgt tgctgatcag gcaagatctt taatcaatga
     1261 aatacccgcc atcgcaccca ttccggcgga cacgaccacc tgtttataaa agccgtcgac
     1321 actttcaccg gccgcataaa cacccggaat ttttgtccgc atccggtcat ccactttgat
     1381 caaccctttt tccatctcca actgaccttc gtataattcc gagatcggtt tttgaccgat
     1441 aaatacaaag acaccgtcaa acggcatatc ctgttcttcg ccggtcagca tgtttttgac
     1501 tctcaagcct tcgacatgat ctttccccaa cacagcggtg acggttgagt tcatcagata
     1561 ttgaatcttc gggttttcct ttgctttttc gagtcgaatc ggatcagccc gcagctcacc
     1621 ctgacgatga atgatcgtta ccgacgaagc gaatttggtc agaaaaacag cttcttccac
     1681 cgcttcgtta tttccgccga ccaccgccac ttttttatcc ttgaagaaaa agccgtcgca
     1741 ggttgcgcaa taggaaactc cggagccggt taattctttt tcaccgggga cggacatggt
     1801 aaccgctctg gaaccggtgg tgatgatcag gttttctgcc agatatattt tcccctccgt
     1861 ctcgatttca aacggacggc cggacaaatt gactttaata gccgtgtcat aatccaaaac
     1921 cgccccgaat ttttcggctt gtgagataaa tcggctcatt aattcggtgc cgctggggtt
     1981 atcggttaca ccggggtagt tgtcaatttc cgatgccaaa gaaatccgac ctcccaaaga
     2041 aggaccggtg atgacaaccg tttccaatcg agctctggcg gtgtaaattg cggcggagat
     2101 cccggccggg ccggaaccta aaatcaaaac tttgcatggg actttttctt tcattgttct
     2161 gtctcctgtt gattccgaag aatcttgcga atcgtttcaa gattttctcg gatgtttcct
     2221 tcaagcgtat tcgccaaaga ttcatccatt acgattttca aaatgccatt ctgttcccgc
     2281 agcgtaaaga tacaaagcgt ctctttgacg ggtagcgggt ttttctcatc ttccttcaaa
     2341 atcgtatcat atatcgccgc catccgttga tcatcctgaa tagtttccgg atcgatcttt
     2401 tgaggatcgg ttttggtatc catcagatat aacatggtag cacggctcat cacttgcaaa
     2461 acatccagca tttgcaaggt gacatcaacg gtaaagagag aagaagtttc atcgttttta
     2521 ttctctttca ccgacaccac tttcatggat tgacggatcg cctctcccaa ccgattttgc
     2581 gggacagtca aagaaacgct cggatcatcc agatagctcg aagctttctc aatatccccc
     2641 gcgcgaacag catcgataaa tgcccgaaca gtctcttctg cgctgagctt gggttgagaa
     2701 ccgcatccgc tcaaagcgaa tatgccgagc aagaagtaaa tcaataaaac agccggtagg
     2761 gttcttttct ttttcattta aacacctttg tccaatcgga aacgccaaac agtttttcta
     2821 aaaaaggggc tcgctcgctt gaacgaagaa aacgaggctg catggtttct tcctgtcgaa
     2881 tcgtctcggt gacatttccg atgtaacgcc cgtcataaaa ataatgacga tcaaaacacg
     2941 aatcccgttc gatatcggaa atctggtcaa agagcaaatt tcccaaccca aaatgaacaa
     3001 aagttttgcc gataaatgcg aatccctgcg gaacatgggc ttgactgccg ctgacaataa
     3061 ccggacccgc ttccgcaatc gcataaaaat catggctttc aaccggcgga acggtgtagt
     3121 catcatattc aaaattttgg aaagtgacca ctaccatcca cccttcttcc ttcagttctt
     3181 tgacttgggc agacagtcga tccatatcgc agcgagccgc gccgggttgg gtagccgtcg
     3241 caaagacact ttccggtccg acggaattgc agccgacaac ggcaatccga ttcccgtgat
     3301 gtgagatgaa aaccggttgc tgcgcttccc cggcattctt cccgccgccg taatatttca
     3361 gattatcttc ccgatattgt tccaaagtga acaagaaggg ctcgatgccc caatcaaaaa
     3421 tatgattgcc cgttaattcg atcagatccg ccccgacatt cttcaaaacc gccgaatagg
     3481 aaggaaggga gcagaatctc gcttcgcgcc gcagcggaat tcccggcgga caatcttcat
     3541 aataagaaac ttcgttgctg atatgggtga aatccgaacg gctgagaacc ggtccgatat
     3601 tccgagtgat aaaatcagcc ccctgatctt cgatggcata agccaaccgg cgtaccatgg
     3661 ctgtcgtccc cgtcagggtc aatgtcgtta atttattcgg atcaaaattg ccggccggct
     3721 gaggaatttg gcaattctta tcagccttca acgattctgt ttgccaggtt acggttaagg
     3781 gataggtttc cggatcaaaa tcgaaatcca gcggactgat attattgact ttcaaaacct
     3841 tccaccgcgg atccgattta tcaaacggaa taatcgccca agttttctct cggctccaag
     3901 ctgtttcaag aatctcgtcc gcgggaaggg ttataacctg atcccgattc ggctctcccc
     3961 aaacagcgga aaaaaccgcg tctgtctcgg catcaaccaa tatttgactt agagaaggaa
     4021 tagcttttcc tgtccaaaaa gattcgatca tttttaacga tacgacatct ctcgaagtgg
     4081 gataaggtgc gacaagcacg tataccagcc ggattttttg gctgttttcc ggacttgaag
     4141 gagcaccggc aacggaaagt gacagggcac aatcggcatt gaccttattc gaaacaaatt
     4201 ttccctgcca gccgagagtt tcgacggcgc ttttcggaaa ggcagcatcc agccaaaggc
     4261 cggtgtcatc cgccgaaacg ggtttcaccg taatcgacag aagcaatatc cataaaaaag
     4321 ccgagatttt gtttatcgaa catttcatca atgaaattat aatcggcccc cttcgcatca
     4381 tgaaatgaga ctttcgggga tatcccgaaa taaccctcca aactcagact ccgtaaacag
     4441 gaaagtctta tgaatcagcg ggattgcagg atttgaaagc attattaaaa atggtactgg
     4501 ctttcaaacc ggtatcgtga ctttcgattt tcgataaata ccgactgatt gaccgtcgaa
     4561 attccaatcc ggttgctttt tcggtgtagt cgatggcttt ggcagcatac aatatgttat
     4621 tctgcgattc cagtcgatta aaatctccga aaaaccatcc gcaagaagta tacattcgtt
     4681 ggcgttcata ctgggcattc atgaaattta acattttatt tttttccgct tcttcccaag
     4741 ccgtcttccc tgtcagccgg atcaattgcg cctcagcggt ttcccgatgg gttaacacag
     4801 ccgaatattc attgcggaat tccagcaatt tcaaatgata cggatccaac gctttctgca
     4861 tttccgtatc gacaatattc gcgatacgat caaatgcccg ccgaataggt gctttccatt
     4921 cgccgtgttc cgcacaagcg cattcgcttg cccaacggct gatcccatga tgacaactcc
     4981 atgaggtatt gttccgtatc cggatacttt tttccggcgg gtaatcacgt aaccaaatcg
     5041 agggaacggt taattcgatg tcgcgattaa ccaatgcgcc cgtggttaac cattgcagaa
     5101 atttatttcg cagcggttga tgatgcccat agagttcccc gtccgaagcg ataagataca
     5161 tccgttcgcg tcgcgaattg tcgggataaa gtggcagtaa atattgatcc ataaatcgat
     5221 ctgcgttgac tgttgcctcg ggatcaaaac tgacacgcat actggtctgc tcttcataaa
     5281 agaaaaccgc gatacgcttt tcctgcggga gatccaccca ataaggtcgg gaggtatcca
     5341 aaccgacacg ggtatttgcc tgccaaggcg ccaatatggt atagcggatc ccgcattgct
     5401 ccattaccat cagggtctcc aaatcgatag ccgtttccgg tgcccataga cccagcggct
     5461 gatgcccaaa acgggcttca aaatccgcaa ttccccattt aatctgagta atcttatcgt
     5521 cgagagtcgc caagggcaga atggtatggt tataggaaag cgccatgccg ttgcccactc
     5581 cgttcgcttc ataaaaagat cgttcctgcc gaacgatctc cttcatcacg tctggggcat
     5641 tttcatccat ccaagtggca agtgtcggac cgagatcaaa actgatcctt ccgaaattgc
     5701 cgtcttccgc attcggtcgg taacattgtg ctaaaatttt ttcattccaa ttatggaagg
     5761 gggttgctcc gggttcctgt ggtattttcc ccgtaaaggg attttcacgc ggcggttgat
     5821 aaaagtggcc gtgaacacta aatgcttgtt ttcgcatcaa acagcctttc tggctttatt
     5881 aagattgttg tattatactc gctaatggaa aatattggaa tatttcggta atttttaccg
     5941 aattcttata attatcctta tttagatact tattcgatat aattactgct tattaatatt
     6001 tcttaggacg cttctgtcag atagcccaga ttccggctca tacgcgtcgg aattagagaa
     6061 aattcccgcg gcagtacatc caaagcggtg atccgatcct ccgcgagata atccgaccaa
     6121 aaatagattt tcggtgcctt ttcaaaccac tgttccatcg caatcagaag cccgcgcagc
     6181 catacgggcg gaatattcaa aaaacctttt ttaacctgaa tttgttccat gattaattcc
     6241 aaacattcac gaatggaaag gatttccatc ccgccgatct gaaacgttcg atgaatcgat
     6301 ttggaatcat ccagacaaag cagcaggcag gtgatcaagt catcaatcca aatcggttga
     6361 atttttgttt ctcccgatcc gggcagcggg accgcaaatg gtacttttcg gactaagcgg
     6421 gcaaaagatt ccgtaaaatg atcacctttc ccgtataccg gagccgtctc taaaatcgta
     6481 taaggaacac cgctggcaat gacagccgct tcgcatagag ccttcgcttg cagaacggga
     6541 taagcggaag ctttatccgc tcccaaatgg ctcagataaa agaaacgctc gacccccatt
     6601 tgcgcagcga ccgcggaaat tgttttcgcc gccggaatat ccaccgtcga atattcggcg
     6661 tcgatccctt tccattcggt tccgatcaaa tgaaaaacaa tttggacgtc tttcatcgcc
     6721 gcccgcaggt ttcgttcatc atcgataccg gaaatcgcta catccactgc gatcccgcgc
     6781 ggaatccgcg gagatttgcc ggatgggtta atcagtaagc gaaccggata tcctaatgag
     6841 accagatttt tagtcaaagc ctgcccgatg aaaccggttc caccggtgac cagaatcatc
     6901 cctttaaata atcgctcgcg ttgtcggatt gctccgactt atctgcggga tcgggagagc
     6961 cggcgctttt cggatccgtt ttcggcttct cttgtgaggg tgcggttcgg ttcggatttt
     7021 caatcgccgg tatttcgaca aacaaccggt cataatgata ttccgaagat ctgacccgtt
     7081 ccgtaaatat ttccgcagct ttttgcatta ccatcaggtt ttcaatccgg aaggaagccg
     7141 atgcagccgg atcaaataca tttccgtaaa aaccgatgag ctccaacaaa actgaaaatg
     7201 tttccgccgc ttccttgttg aaccataaga cttcctgata agtgttggtt cgtaagacgt
     7261 gccggacaaa gtcggatttg agcatctcat tcaaccggtc atccagtgtt ttgagatccc
     7321 atttttgaaa atccgccaaa gtggtataca accactggat aaggttgatt tgttcgctga
     7381 cagaaagatg atcggtgaag ccggcttccg tgaggattct ttcttgggct ttgtataaat
     7441 gccagtcatc aagaatattt tctactttcc cgcttccgac ttctcctctc tcaaaacgcg
     7501 ctaacagcac ctgctgcaga ctgatgaaaa gcgcaatcca aagttcggga aaatcggtaa
     7561 tatctttttt gattttgctt aatgcggcat tatagcgagt cattccggga atcatcttcc
     7621 gttcggggtc ggtgagaaat tcattaatac cgtagcaata gacgttgaac aagccttctt
     7681 tggagcggtt caggtcataa aagatattga atgcatccat caatttttca aaatcgggcc
     7741 atgtgccgtt ttgaatttcc aaccattcgg attcggtcat tgccaagccg attttaccgt
     7801 ataaccagcg taactttcgt ccgttaaaac aatttcgata gggatccaaa acatagtgat
     7861 acagcagttc ctgaatggta tcgttgagat ccaacgtgcc gcttccgttc aaccaactgt
     7921 tcagttttcg ccaggtgcct tcggaatcat cccttacctc atatacatca ttgaatacat
     7981 agtattggta agcgtaaagt tccagataaa gtccttcccg ccagatttgt tgagaaggcc
     8041 gaacatagtt cattccgctg gcggaatcat gaaacacgac aaaacattcg ggatcgttat
     8101 gaaggtgcaa actttccgaa agcatttttt gggtggtgta tttttcaccg tcggattgtt
     8161 tgaccacaaa gggaaccgac tgcatgatat accctttggt atccccaaag cgattgttat
     8221 agactaccaa tgaatgatga ccttcgccgt aattggaata agcaaagaca ttttcatcga
     8281 cattaccgta tgcggtcgca aaattataga aaacaaaatt cgtcacttcc gcaaagcgtc
     8341 tacgctgatg taacaacggg aatatttccc gttcatgccg gtgcaacagc tccgtatcga
     8401 catactcatc ccaataagct ttacgatatt ccataccgta tttctcttta aagccctgaa
     8461 tctgaccatg cccaaacatc ggcagccccg gcatggtaac catcatggta caaatcccaa
     8521 aatatttgtc cccttttccg aattgttcta ccgcggtttt ctcatcggga ttgttcataa
     8581 aattgacata gcgtttaaga atatccggat caaattccaa tgtgcttttg aggagaatcc
     8641 gatatttggc attatcttca tcccgtaaca gattcataaa cgcactgtta taaactcgat
     8701 gcatccccaa ggttcggaca aaataacctt ccatcagcca gaacgcctca gccaataaca
     8761 gcgtgtccgg agcttccgcc gcaacccgat ccactacttc ccgccagaat tccatgggaa
     8821 acgctttatc aaaatcagct ttggtcatgc tgaattcggc acgggtcgga atagccccac
     8881 ccgtccccgg ttcggggaac cataaccgtt gataatgttt ctttgccaat gtcatggcgg
     8941 catcaaaacg gataatcgga aaccgtttgg cgacctgcaa aatggtttga ataactgttt
     9001 cccgcacttc cggattcaga taattcagtt gggctgtatc attccacggc atgctggtgc
     9061 cgtcatttcc gtgatagata taacggacat cgccggtcgg ataatcaacg cgttgaaaaa
     9121 caaccgccgc atcctcccgt gtgtaataat ggtcttctat ataaatcccg atattcgggt
     9181 caggtgaata gttgggacca ttaaaagtgt aggaaggaaa aggactgtag ggaagagaaa
     9241 gaaaacgatc gggatgatat aaaacccact cagaatcgat ccccatatga ttcggtacca
     9301 tatcacttgc caaacgaatc ccatagcgcc aggctttatt cttcaactcc cgaaaaccgt
     9361 tttcgccgcc gagatcatcc gcaatgcgat agtcggcaat ggagtaagcc gaagcgagcg
     9421 cttccggatt tccgcaatat tgtttgattt ttgcggaagc tttggatcgt tcccaaacgc
     9481 cgattaacca cagaccggta aatccctgat cgcgtaaagt gcttaattct tcttccggaa
     9541 tttcgttcag tttggtaatc ggtcgatggt attgtttgga aagttgatcc agccaaacat
     9601 aggtattttt tgcgatcaac accacccgcg gcatccagtc ccgatcttcg ctgaaagctt
     9661 catattcccc tgcttcaaga gatccgtcgt tatacaccgg aatttcaacg ggtcccggtc
     9721 cgacaccttg cgccttgctt tcttctttca tccaatccag agacatcagc aaccggcgca
     9781 gaaaaatgcc caaatgggtc ccccaacggc tgcggataaa ctcaagttga tcaaataaag
     9841 aatcgggatg ctgtttgatc ggttccagca gaaaatccac taaggtcatg ccatccactt
     9901 ttggaagcat ttgaaaacaa ccttgaattt ccgatattaa gtcgttataa gccgtggagt
     9961 tttccaactc tttgtcatca aacaactctc ccaagggctt catcgccgcc ggattctgat
    10021 ttgttatcca tagcaagagc atttcttcaa tcgcttcata acgattaagt tttcctttag
    10081 gattcacttc atcgagatac tcttcaacgg tgatttttcc ctgatataca tccaacgggg
    10141 gaaattcttc gcaaaaagcc ttcagtgttt cattaagagc ttccttgcct atcttctctt
    10201 ccagccatcg ataaatctgt tgattcaatt ccggcacgta gttttcacga taaagatcaa
    10261 tcaaataatg catgatttca tcgattaatc ccagtgaatt caccaatccg gcgtataccg
    10321 gttcgtgatt ccccgttttt aggcggacat cgttgatttt ttgagtaaat tctcggaccg
    10381 cttgaaaatc agcaaaaatt acattcccgt catatcggaa aagacctgaa tcgaactgat
    10441 aacgaatcct ggattgagaa gatatatgga attccatgtg atcatccttt ctgattcggc
    10501 aatatgtcag tcatcgccat agcatttagt tattctgaaa agttcgaggt tcattaatct
    10561 tcaaaatatg agtataaaca ttcgaaccgc ttcctccgat attttgagtc atcgcaattt
    10621 ttgcgccggg tacttgattt tccccgcata agccgtataa ctgcaacacc gcctcgacga
    10681 tttgatagat tccggttgct ccgaccggat gaccgcgaga ttttaatcct cccatagtcg
    10741 cgaggggtat tttgcctttc ggaccgatga ttgcgggatc cgcaagagat ggcccttgtc
    10801 cgcgctccgc aaaaccgctc gcttccaatg agagagcttc aataatcgta aaagcatcat
    10861 gcgcttcaaa cagatcaata tctgcaggtc cgattcccgc ctgtcgataa gcctcttttg
    10921 cggaccgttc ggcagcggaa agccaaagca tatcccgcct gtcttgaaga gctatggtat
    10981 cggtagctgc ggcagagccc gctatcgtaa caaacttttt cccctttatc agcttttcaa
    11041 ccgggactaa gagtaccgcc gccgcgccgt cactcattcc ggcagcgtcc atgacattaa
    11101 tcggatcgca gaccggcggg gatttcatat atgcctccgg tgtaatcgga aaaccgaaac
    11161 gagcgttggg gttatgcgct ccgttagcat gggcattaat ggaaaatccg gcaaaatctt
    11221 ctttcttcca accatatact tccatataac gacgcatcaa taaggcattc aatgccacaa
    11281 atgagacgcc catatctcgt tcataatcgg catccgctgc ggttgccaat tccgcggtaa
    11341 tttcagcgga cggggaatcc gtcatctttt caacaccgac ggcaatcgcc gtatccgctt
    11401 caccggaact gaccgccatc aatgccgccc gaaacgctga tcctccggag ctgcaggctg
    11461 attccacatg aacagccgaa gcattcccgg cacccaccca gtctgcgata taagctccta
    11521 aatgaagctg atgattggcg gagccggtca tcatattccc tacgaaaata ctgtcgacag
    11581 tcgaaatgcc ggtcttttca aagatttgaa ggatgggttc tcctgccaat tccagaagac
    11641 ttttatccca atgttcactg acaggagttt gaccgatttc taaaattgct acttcgcgca
    11701 taaaaggagg tttccttttc ttttctgtta ttgatcgatt cgaggaaact gttcggtcag
    11761 tattccgacc taaacggaat ccgataagca aattataatc tacgcactat ctccctgaag
    11821 tgtggaaatc gctatttgac ggtttgatag ctatcgaagc ggtaaaatca gctcaattta
    11881 ttttttctgc tctgcattga tcaaattaca gaactgcttt ttacggtaat cggccagccg
    11941 ttcagaaaga tcaacctctg atagaataga ccagaggaaa aatggtgcag actaataaaa
    12001 ttgagctggg aaaaatcggt gaatcgattg cgttggaaac agcaaaacgc agaggatatc
    12061 aaatggttgc gacaaatgta agaacccctt acggcgaaat cgatctgatc ctgaaaacaa
    12121 cgcatcaact gatctttgcc gaggtaaaaa ccagaacctc attttccttt ggatatcccg
    12181 aacaggcgat caccacatta aaactctctc atatgattca atctgctcaa ttttatatgt
    12241 tacaaaataa tcttcgggat gaatacagaa tcgatgtcat ttctattctg tatgattcga
    12301 acaaacatca ggttgcgaat ttggaatgga tagaaaatgt cacagcatcc gagtaaaatt
    12361 cccgttacgg taatcgtcgg tcccaccgct gtcggtaaga ccgcttttgc gatcgagtta
    12421 gccaaaattg ccaatgcgga gatcgtttcg gcagattcac gctattttta tcggggaatg
    12481 gatatcggta ccgcaaaacc gacaccggaa gaacgtcaaa gtattccaca tcatttgatt
    12541 gatgtcgctg atcccgatga aacttggagt ctggctttat tccaacagaa agcatacgac
    12601 gcaatagcct ccatccacga acggggaaag aatgtaattg tcgtcggagg cacaggccaa
    12661 tatatacggg ctcttttaca aggatggagt ccgccaacca tggaagccga tcttgtgatg
    12721 cgagcagtac ttgaagattg ggcaaatgag atcggtccca tggaaatcca taaaaagttg
    12781 gcgattttgg acccgatagc cgccgaaaaa atcgattatc ggaatgtaag acgaacaatt
    12841 cgtgcattgg aagttatttt cggcagcgga cagcgctttt ccgaacaacg gaaagttacg
    12901 gaaagtcctt attcatttct gatccttgga ttgaaccgaa gtcgttcttc cctttatcag
    12961 cgaattgatc aacggattga tcaaatgatt cggcaaggat tcattgaaga agtcgcaggt
    13021 cttttggaaa agggttattc gccggcgctt ccttctatgt ccgcaatcgg atatcgtgaa
    13081 atcagcgctt atttgaatca tgagatatca ttggatgagg caatccggtt gattaaacaa
    13141 cggactcgca attttgtccg gcgtcaggca aattggttca aaacaaacga cccgcaaatt
    13201 cattggttta atttggaagg ataaacgggt attctgatca tcgaatgacg atataattta
    13261 ttcagttaaa agaagaaaaa aatgcaagaa aatttatctc aaaaaccaat atatgattca
    13321 gccaaactta ccaacccgtt ttttgaggaa ataaaagggc tgaaagaaaa cagtttcatt
    13381 ctgcgccgaa tgatttatac caactttaag actcgctata agcgatccta tttaggcgta
    13441 gtttggtcaa tgctgaaccc tttattgaat atgattgttt ttgtaatcat attttccaat
    13501 cttttccgat ttgatgttcc acactatcct ttatatattc tttccggaaa tatgattttc
    13561 acattctttt ctcaatccac ttcggaagcg atgatgcaaa ttattgcaaa tgctggcatg
    13621 ctgagaagag tttatttacc gaaaacagtt tatattcttg cgggaatcgg tatcaatggc
    13681 atcaatttaa ttctaacttt tattccgttt attttgattg ctttaattga tcgattagct
    13741 ctttcatgga ccgtcattct gatcattccg gtcttatttt tattatctct ttttgttgtt
    13801 gggataagtc tcgtagttgc aacaatcacc ccatttttaa atgacttttc gcaaatttgg
    13861 tcggttatta tcacattatg gatgtattta acgccattgt tttatccggt aaatatcatt
    13921 ccggagcatc tgatcggaat ctatcaacta aatccgatgt atatttacgt cactttattt
    13981 cgggatccgt tgctttatgc aaaaataccg gaactttctc tatggttagg tggattgatt
    14041 tatggcgtaa ttatttttgc tttcggatgg tggcttttta caaaaaattc caatgaattt
    14101 gcatataggg cataagaatt ttgatggatc aaaatatcgc aattcaaacc gaagatttgg
    14161 gcatatgctt taaaatgcct tccgaaccca tttcgggtac gaaagagtta tttatcaaaa
    14221 tcgcaaagaa aaggattaag atcaatacat tttgggcgct ccggcatgtc aatctttcgc
    14281 ttaatgaggg agaatcgatc ggaattatgg gaaaaaacgg tgccgggaaa agtactttac
    14341 tgaaagccat agcccgcgtc ctaactccca aagaagggcg agtatatgtc cgcgggaaag
    14401 tcttcccaat gctcgaactc ggtgccggtt ttaatatgga attaaccggt catgaaaata
    14461 tttaccttta cggaaatatt ctggggttga caaatgaaaa agtcacccaa ctttacgaca
    14521 gtattgttga attttccgaa ttaagagaat ttattcattc tccgttgcgt tcctattcaa
    14581 ccggaatggt tgcaaggctg gcattcgcca ccgcaaccgc tgttcaaccc gatattttat
    14641 tggcagacga gattttatcg gttggtgata aagattttca agataaatgc atggaccgca
    14701 tgcgtagcta tttaaataac ggaacaacaa ttgtatttgt ctctcacagc ccgaattctg
    14761 ttaaacaaat atgtaagaaa ggattatggc ttgaacatgg acaagtaaga atgaccggga
    14821 gtgcggagga agttgccgat gcttatgctg ccagttaaaa ttacaaataa aataggatat
    14881 taataatgga ttttatacct ttatctattc ccgatgttat tttgatcaag cccgatgtgc
    14941 atcgcgatga acgcgggttc ttttttgaaa gttggcaaaa aaataaattc gcaaaagccg
    15001 gaattgatca ggagttcgtt caagataacc attcgcgttc ttcaaaagat gtactcagag
    15061 ggttgcatta tcaaattcga caaccacaag gaaaattgat tcgagcaata gtaggtgaag
    15121 ttttcgatgt tgccgttgat ttacgtcgta gttcaccgag tttcggaaaa tgggtgagcg
    15181 ccaacctcag ctcagaaaat ttcaatatgc tatggatacc tcccggcttt gctcatggtt
    15241 ttttggtttt aagtgacgtt gccgaattcg tttataaagc cactgatttt tatgcccccc
    15301 aatacgaaag atgtatctta tggaatgatc gagatttggg aatagattgg ccgatttcgg
    15361 gacacgaacc aattttatct gaaaaagatt caaagggtaa actatttcga gaagctgagg
    15421 tctatttatg aaaaatgtct tagtcaccgg gggcgccggt tttatcggct caaactttgt
    15481 ccgatattta ttacaacgtg aaccggacgt caaagtcgta actttagatc tcttaaccta
    15541 tgccggaaaa acggaaaatt tggaggactt gccgcatcct gaaaaccatg tctttgttca
    15601 gggagatatt tgtgacaggg aattggttga tcagcttctc gccgagcatg cgattgatac
    15661 gatcgtacat tttgccgcag aatcacatgt agatcgatcg attttaggcc cgatgccgtt
    15721 tatccagaca aatatcatcg gtaccttcac attactggaa gcagcaaaaa atgcctggaa
    15781 agataaaccc ggcgagtttc gttttcatca tatttccaca gatgaagttt ttggcgctct
    15841 tcattcgggc gatccggcat tcgaagaagg gacaccctat aatcctcatt cgccttattc
    15901 agcatccaaa gcatccagcg atcatttggt tcgggcgtat tactcgacat ataaccttcc
    15961 cataacgatc agtaattgca gcaacaatta cggaccttat cagttccccg aaaagcttat
    16021 acctttgatg atattaaatg gcataaccgg aaaaccgtta ccggtatatg gaaagggtga
    16081 acaaatacgt gattggttgc atgtgatgga tcattgcgaa gcaatttacc tgattctgaa
    16141 aaaaggaaaa atcggtgaaa cctattgcat cggcggagat aatcagccga caaaccttga
    16201 aattgttcaa ttaatctgtg aaattcttga cgataaggtc ccgagggctg acggaaaatc
    16261 gcataccagc cagattcaat atgtttccga ccgtccgggt catgatttcc gctatgatat
    16321 caatacaaat aagattcaac gggaattggg atggaaaccg gctcatgcgt tacgtaccgg
    16381 attggaagaa acaatcgaat ggtatttgaa tcattccgct tgggttgatt ctgttttgga
    16441 caaagatgaa ttttcatcct ggataaatac caactatcaa gaaagagatt catcatttta
    16501 aaaagagaag gaatttttct tctcttttaa taatcgattg acaaaataaa aggaaagata
    16561 gcaaatgaaa ggaatcattc ttgccggcgg aaaaggcaca cgtctctacc cgctgacccg
    16621 ggttgtcagc aaacagttat taccggtatt tgataaaccg atgatctatt atccgctgtc
    16681 gatgttaatg tttgcagggt tgaaagaggt acttgtaatc agtacaccgg aagatcttcc
    16741 cctttacaga cggcttcttt cggatggcag ccaatggggc atgaaatttg aatatgccga
    16801 acagccgaaa ccggaaggtc ttgcgcaagc cttcctaatc ggtgaaggtt ttcttaacgg
    16861 agattcggca tgtctcgtct tgggagataa cattttttat ggttcttcca ttccaaccaa
    16921 attgcgtaca gctgctgcac ttgaaaatgg agcggtcatt tttgcttatg aagtgaatga
    16981 tccggaacga tatggggttg tcacctttga tgatacaggt aaagtaatca gtttagaaga
    17041 aaaaccggcg caacccaaat cgaattatgc tgttcctggt atttattttt atgatcaaga
    17101 agtgacaaat tacgcaagaa atttaaagcc atccccaaga ggagaactcg aaattactga
    17161 tttaaaccga atttatatgg aacaaggatt actgtcggtt caggtattgg gcagaggagt
    17221 ggcatggtta gatgcaggca ctcatgaaag tctgattcaa gccggtctgt atgttcaggc
    17281 tgttgaatcc aggcaaggct tacaaatcag ctgcccggag gaaattgctt ttcgaatggg
    17341 ttttatcgat gcaaaacagt tattaaaact cgcggaggac gcgggagatg cctcattaaa
    17401 aaaatacctt cggagaattg caagagaata ggtttaaaca gccgattttt tatttgtttg
    17461 atcgcaagtg actcttcatg atcaatcacc gacaacttca ataaaggggt tttcaatgaa
    17521 aattttactt atcggagaaa aagggcaact ggcttgggaa ttagctcgta cgttaatgcc
    17581 gatcggtgat atcgttccgg tcgattatcc tgcttttgat atcagcaaca aagaacaaat
    17641 tcttgaaaca gtttattcgg ttgttccgga tttagtaatt aatgccagcg cttatacaga
    17701 cgtcgatcgt gcggaaaccg aaaaagaaaa agctatggca atcaacgccg aagggcctgg
    17761 ttggctggct gaaatttgtc gatccaagaa tattccggtt attcattatt ctacggattt
    17821 tgtttttgat gggatgaaaa aatctcctta tgtcgaaaca gacataccga atccgattaa
    17881 tttttacggc cgaacgaaat taaaaggaga agaagcagtt ctgaatgcca cggaatccgc
    17941 tttaattttt cgcctggctt ggctttattc ttctcgtggt aataatttca tgaaaaaatt
    18001 tttagaatgg gctgggaaat atgaaattat caaagtcgtt gatgatcaag tcggaaaccc
    18061 gacttgggca cgttttgtag ccgaagcaac agcatccatt ctggcggaat cattatcatc
    18121 ggatcttcct ttatataact ggataaaaga gcataagggt atctatcatc taaccggcag
    18181 cggatataca aaccgatatg aatggagctg caaaatttgg gaatttgctc caaaccgaga
    18241 aaatctaaaa ataaaaaggg ttgagcgtgc aaaaaccgtt gatttcccga ctccagctgg
    18301 ccggcccgcg tattcggcat tagattgcag tcttgtaaaa agaacttttt caatcaatat
    18361 tccaagttgg gaaga
//
LOCUS       DAOSMM010000046        18278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3060_length_18278_cov_18.473907, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000046 DAOSMM010000000
VERSION     DAOSMM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 18278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18278
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3060_length_18278_cov_18.473907"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1814)
                     /locus_tag="PK898_07375"
     CDS             complement(147..1814)
                     /locus_tag="PK898_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19891.1"
                     /translation="MEKNQRITCAFIILIGLVYFCLLIPANLTGAADPEMLSVFEVDE
                     YAQYPHVIQMLTPGDTLYHSIRNFFIYQHYFYGFPFYFLSAISVFPLKLLSPNWPENT
                     RLIVCWLRMTINVLPMIFAIGIFTWLITRFKNRLISLSLFVFLLTIPAVLLNNFWWHP
                     DSLSLFFISLVFLFLDLDEFRFGKYFSLAAAACGYTVGIKYQGLYFALAIPLLLIYAI
                     LRKRLSWKKAIKKAIIFVVIMMVFFIFSNPLLLLPQERAEIIRTQQIQFEQTGVGIFT
                     IHSNALRNSLLLPSDIRKYYAETWFFILAIIGLLMGFKGEEKQRIQSLLFLSYLMVAF
                     CITTYAATNRLHYFLPVAVPLLGFIADFYFIGKRDFPAIDLKFGTNIRKVMYGILTLL
                     IAFQFSLNIRLDHSLYRDQLSRETESSSIALYHQIENEIIPVLKSRVNNRMIRVFRDW
                     KVYFPDQEGVAVQMDWNMATLDKINEWQPDLIILEEENLKEFSDPTIIDHAVNPEEMK
                     LIHSFYQQAASDQIPGYDRIMQNSFGSVYVRVGMRDALKKDCRCEFP"
     gene            complement(1907..3454)
                     /locus_tag="PK898_07380"
     CDS             complement(1907..3454)
                     /locus_tag="PK898_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19892.1"
                     /translation="MRGKKRISFFLLLSVSFFIFLIFCTSISIFAASSDQQIVWQLAS
                     WNTSLTVCTLMINSEIQPGEQEILEQCGPEILFEWQTTPPCDHIIENQNCTGLFLRRT
                     GSIVSQNEIPDEKSQDSIGSFPPLRFELVDCLPSQLCQQIPEIQIRMVDSSAFTSFPI
                     KIRIRMGTFEGECESSECRVPLRMTGEQGEWLYYWTMNSNGVQSVHHRVKYRVLPDRS
                     DPTKYHFDLLSRSYPDFIPPGSDLWGLFPASYKPVSKMLIKPDSKDDLITNKPYDILC
                     GKMIWHGLVDTSRCPGFGLMGGESANACGLESCSGKLFEWQNKQNDRFYETGKKYNVP
                     PRLVKGMVAQESQFWPESDIEGEYGLGRITILGIKMLLDWYPAYFNQLCYAIFKMQPN
                     RCGSCFSEMETKDQNVLIGSLIAKTNSAEEIDLITAAVKASASQIEQIILNTSEFPLE
                     EITDYESLWKIAVANYYAGSGCVQRAITSTYLYGYPLIWDTVKNYFGYGCEKAVEYVD
                     RVYSLWE"
     gene            complement(3719..4534)
                     /gene="murI"
                     /locus_tag="PK898_07385"
     CDS             complement(3719..4534)
                     /gene="murI"
                     /locus_tag="PK898_07385"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOU19893.1"
                     /translation="MNKSAPIGIFDSGVGGLTVMRKLIQGMPEENIIYFGDTARVPYG
                     NKSKETIIHYTRQIVRFLESQNVKAIVIACNTASAYALDDIRKETEIPIIGVIEPGAY
                     AAAAATRNGKIGIIGTYATIHSGAYESFLSQINPDFEIYKQACPLFVPFVEEGLFEDD
                     LTDQIIARYLTEIKRENIDTLLLGCTHYPLIKTAIERFMGENVICVDPGLNTAEMLRQ
                     ILTKNNLLNTSFSQEKANYLFYVSDPAARMTEFANQIMPFSIGKIQKINIEEY"
     gene            4733..6031
                     /locus_tag="PK898_07390"
     CDS             4733..6031
                     /locus_tag="PK898_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU19894.1"
                     /translation="MEPGSCTPYEFNTHYLTFLIVFLFLYFLFTVIYSVFRFNRRPDI
                     FSEENEKEKDTENASALSPEQVNVIWLAAKINAVIVSIFAFILINDSFCLYRSQWLLL
                     LSIILIPLVLYFLEALISGIAARNADSWIKYFHGIARLTVFFFKPLYSVYESFKPDNI
                     RNDYTFNEVEAHLREWVNNAPDNAALKEDERKMVRSILHFSDTLIREIMIPRIDMTAV
                     DVETSLEEATSIVLASGHSRLPVYEDDIDNIIGVLYAKDLLKIYTENPENRALRDYLR
                     PALFVPESKKAGDLLSEMQTSGIHMVIVVDEYGGTAGIVSMEDIVEEIVGEIRDEYDD
                     SEEKLINEISENEYSLLGRIDLEDVNEILGTHITRESADTLAGFLYSQIGKVPVGDEE
                     IEVEGWIFRIEELSGNRIRRVHVKKLEEDSPNKEGEQDEE"
     gene            6021..6425
                     /gene="cdd"
                     /locus_tag="PK898_07395"
     CDS             6021..6425
                     /gene="cdd"
                     /locus_tag="PK898_07395"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOU19895.1"
                     /translation="MKSENDLRELIDIALAVRGKAYAPYSKYAVGAALRTGSGKIYTG
                     VNVENSSYPLTICAERNAIFHAITEGDSDFETIVVATENGGSPCGACRQVMAEFALDM
                     NVVMVDKSGKIILQATVDELLPEAFTPKSLRK"
     gene            6432..7181
                     /gene="recO"
                     /locus_tag="PK898_07400"
     CDS             6432..7181
                     /gene="recO"
                     /locus_tag="PK898_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOU19896.1"
                     /translation="MERSVRVQALVLSHNDFGEADRYVKLLTPDRGKISALAKGVRKM
                     NSRKAGHLEPFTIISAQLVQGKGSSWIISQVSTIESFPGLTDNLESTCHAAYVSELTD
                     RFATEEYSNFELYKLAVETMRRLSKITDHFPVLRYFDFRLLDLAGFRPQLHSCVRCGN
                     EIIEEQQFFSNILGGVVCPKCGMKESGIVSISSRSLKYFRYYQTHNFSQAAAAGWPAD
                     LRQESEIILTNYLTYFAEKKINSQIFLNCIR"
     gene            7230..9677
                     /locus_tag="PK898_07405"
     CDS             7230..9677
                     /locus_tag="PK898_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOU19897.1"
                     /translation="MRYGTFDDEKLEYCITQPDTPLPWINYLGTEDFFGLISNTAGGY
                     CFYRDARLRRLTRYRYNNVPADSGGRYLYLRDRQTGDYWSPTWQPVRSTLDHYSCRHG
                     LGYTVIASEKNGIGFSGCYFIPIGENLEIWDITITNRRKEEADLSLFSFIEFCLWDAQ
                     DDATNFQRNYNIAEVEVEGSTIFHKTEYRERRNHFAFFHCSDPIAGFDTQREAFLGRY
                     RGFDQPQAVEQGVSFNSIATSWQPIGSHQVQFHLSTGESRRIIFLLGYHENPRGQKFD
                     PPESNTLNKKTVYPIIREWEHPERVDRAFSDLKSYWKGILQKFSVRTPVNHVDRMINT
                     WNAYQCMITFNLSRSASYFESGIGRGMGFRDSNQDLLGFMHMDPDRARQRILDLAATQ
                     MENGGAYHQYQPLTKRGNNDIGSGFNDDPLWLVLSTSAYLRETGDYSILEESIPFENR
                     AGSEQPLSEHLDRCISYTIERIGPHGLPLIGRADWNDCLNLNCFSDSPGQSFQTTTNR
                     EGKNAESVFIAGLFVLAANAYADILDILQQTEKSSRIRSYANEMKKVADTAGWDGRWF
                     LRAYDDQGAAVGSHRCTEGKIFIEPQGICTMAGIGLDNGHAAQALDSLNTYLSGKDGI
                     ALLWPAYTHYYINLGEISSYPPGYKENGSVFCHTNPWIMIAETKLGNGDRALDYYLKI
                     CPSFREEISEIHRCEPYVYAQTIAGPESPTPGEAKNSWLTGTAAWNYVAITQYILGIR
                     PELTGLRVEPIIPKDWEGFQVVRDFRGVRYDISVKRKGKGNHCQLNIDGQDIEGTVIL
                     SSIVKGKKEVTVQAIIQ"
     gene            complement(9736..11481)
                     /locus_tag="PK898_07410"
     CDS             complement(9736..11481)
                     /locus_tag="PK898_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU19898.1"
                     /translation="MINRKLLLLFSITLISLMLIAMPVLADEAKYFRTYFGSGDIPSI
                     DQALVTDFIGIQLVDEMTVGIMRQNETTGLLEPGMATEYSVSDDGRVYTFKLLQNVPW
                     VRYNPETKAVEEVKDCDGNVRYVTAHDFVYGAERTLRPETASGYAYVANLIEGAEAYN
                     TPVEEGEGTPVARPDFSTVGIKALDDFTIEYTFKEAGVFNLNILSMWMLHAMPSWIID
                     GDACNEALGERWTETGFYQGYGPFTLEEWVHDSHLTIIANPFWPGTEFVPKPKIAGVH
                     YSLIDTSAALAEYEAGNMDEAGIPSADYDRITSDPAFKDQIKSSTDSVGTEFLIYNEW
                     LPPTDDVRIRKALSLAVDKEAVVKTLKEGIAAPFYIHPGVDGAPKVEDYPDLGIMYDP
                     EAAKALIDEYCKEKGIQPKDIVVTYNFNTSESRKMLAETIQYMWQNTLGITVNLKNSE
                     WAVYKVERQQGLDNVYRSTWVQDYMDANNFTADVFLCGTGYSSVTDWPSKDCTDKSNP
                     AYIEYEEVVKKAGKETDPKARAALYARSDEIIIEEEAIINPIYWYSSLVLRKPNIIAP
                     ETLTGYAHWEKWEIK"
     gene            complement(11973..12155)
                     /locus_tag="PK898_07415"
     CDS             complement(11973..12155)
                     /locus_tag="PK898_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19899.1"
                     /translation="MDQKIIEIKDELVRMRKDFHRFLELGFKEYWTAEKAAGYMKNLG
                     REVQTGIGGTGVVKLL"
     gene            12368..12441
                     /locus_tag="PK898_07420"
     tRNA            12368..12441
                     /locus_tag="PK898_07420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12402..12404,aa:Arg,seq:gcg)
     gene            complement(12666..14333)
                     /locus_tag="PK898_07425"
     CDS             complement(12666..14333)
                     /locus_tag="PK898_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU19900.1"
                     /translation="MNKKVIIVGGVAGGATTAARLRRLDENAEIILFERGKYISYANC
                     GLPYHVGDVIPSREDLLLQTPESLKSKFNIDVRIQQEVTFIDRANKTVSVKNLATSQE
                     SVESYDTLVLATGSSPIIPNIPGIRSKYIFSLWTVDDADHIRQYIRQNKISDAIIIGG
                     GFIGIEMTENLRRLGITVSLVEMLNQVMAPFDFEMAQLIHKTLEENGVSLYLGNGVTS
                     FTETDESILVNLSDGKQLATRLVILSIGVKPNSELARSAGLALNARGGIVVDKTLRTS
                     DPDIFAVGDVIEVEDFISKGKTMIPLAGPANKQGRIAAGNIAGENDQYPGTQGSAIVK
                     IFDVCAANTGANEKTLLKNGLIKGKDYEKLIILQNSHAGYYPGASPMAIKLLFSTDGN
                     KIFGAQIIGKEGVDKRIDTIGTAIRLGGTIWDLKSLEFAYAPPFSSAKDPVNMAGFVA
                     ENILKSRVFFAEWDVVERHDPEKTILLDVREDAERRTYALSGALNIPLGQIRSRLSEI
                     DKEKEIIIFCAMGVRAYNAARILMANGFQNVKVYPGGMRFFRATHYK"
     gene            complement(14892..15014)
                     /locus_tag="PK898_07430"
     CDS             complement(14892..15014)
                     /locus_tag="PK898_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19901.1"
                     /translation="MADTGKNDKAKKEQKKKPKMTIKEKRKLKREKTNKETGAV"
     gene            complement(15336..16460)
                     /gene="wecB"
                     /locus_tag="PK898_07435"
     CDS             complement(15336..16460)
                     /gene="wecB"
                     /locus_tag="PK898_07435"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOU19902.1"
                     /translation="MNNRLKVMVVVGTRPEAIKMAPVIKCLQAESHIETIVCLTAQHR
                     EMLDQVLQIFSIPPDIDLNLMTENQTLSDITASILIQMNKVLLERKPDWILVQGDTTT
                     VMAAALAAYYNQMKVGHVEAGLRSWNKWAPFPEEINRKIAGVIADLHFAPTEMARQNL
                     LREGIPPEICHVTGNTVIDAQQLALKIPFEEKGTPLEGIPFGEKEIITVTAHRRENHG
                     KPLVDICNAILKISQVYADRVHIVYPVHLNPIVRKTAFSILDHQPNISLIPPLDYLSM
                     VYLLKNSKILLTDSGGLQEEAPGMGVPVLVLREVTERPEGVISGNVRLVGTDPENIYT
                     HVCDLLDHPDQWEKMSKATNPYGDGKAAERIVKLINAEQK"
     gene            16661..18184
                     /locus_tag="PK898_07440"
     CDS             16661..18184
                     /locus_tag="PK898_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOU19903.1"
                     /translation="MLAKVYSCTVTDLDGDIIEVEVDNSSYGLPQTVIVGLADTAVQE
                     SRERVRSAIRNTGLEYPRKKVTINLAPADLRKEGPFFDLPIAIGILAANRIIPLTAFE
                     NSLIIGELSLDGSVRHVRGILPITAKAKESGFSRIYVPACDAREASLIPDIDVFPVTS
                     LSVLYHHFIGTEQIQPAEPMKITQAIPEVLTDFKDIQGQEHVKRALEIAAAGSHNILM
                     VGPPGTGKTLMARALPSILPALTVQEALDVTRIYSIADQLPSDRPLIQSRPFRSPHHT
                     ISNVGLVGGGNHPRPGEISMAHHGVLFLDEFPEFGQRVLEVLRQPLEDKVVTISRASG
                     SVTFPANFMLVAAMNPCPCGFYGDPVHPCHCSNLAISNYQKRISGPLMDRIDIHVDVP
                     RIEFQKISSQKDQEPSAAIRARVEKAREIQRQRFSESKNISCNAEMRPAEIKKFCQPD
                     DEGNRLMRLAMDRYELSARVFHRLLKLARTIADLDGSEKIQSNHIAEAIQYRPRQTE"
BASE COUNT         5219 a         3836 c         3781 g         5442 t
ORIGIN      
        1 tttccgacat attccctctt tatttttgct acctaactgt aaaactcagg aaaaaaagaa
       61 ttagagctaa agtagaaaat tcttttaaac cgtgggtaat ataaattctt ctcgaacgaa
      121 aaagtgttta tccgtataaa aaatcatcag ggaaattcgc atcggcagtc tttttttagt
      181 gcatctcgca tgcccactcg aacataaacc gatccgaaag aattttgcat tatccgatca
      241 tatccgggaa tctggtccga agcagcttgt tgataaaaag aatgaatcaa tttcatttct
      301 tcggggttga ccgcatgatc gattatggtg ggatcactga attccttcag attttcctct
      361 tccaaaataa tcagatccgg ttgccattcg tttattttat caagtgtcgc catattccaa
      421 tccatctgaa ccgcaactcc ttcttgatcc ggaaaataga ctttccaatc ccgaaatact
      481 cgaatcatcc gattatttac gcggctttta agaacaggaa taatttcatt ttctatctga
      541 tgatataagg cgattgaaga agactcggtt tctcttgaga gttgatcccg atacaatgaa
      601 tgatccaacc gaatgttcag actaaattgg aatgctatca aaagggtcaa tattccatac
      661 ataactttcc taatatttgt accgaactta agatcgattg ccgggaaatc tctttttcct
      721 ataaaataaa aatcggcaat aaaccccaat aagggtacag caacgggaag aaaataatgt
      781 aaccggtttg tcgcagcata agtggtgata caaaaagcaa ccatcaaata cgataaaaat
      841 aatagagatt gaatcctttg tttttcttct cctttgaatc ccatcaataa accgattatt
      901 gccaatatga aaaaccaagt ctccgcataa tacttccgaa tatcagacgg caataacaaa
      961 ctgttacgaa gtgcattcga atgaatcgta aatatcccga caccggtttg ctcaaattgt
     1021 atttgttggg tgcgaatgat ttccgctctt tcctgaggca gtaacaacag agggttggaa
     1081 aatatgaaaa aaaccatcat gatcacaaca aatattattg ccttctttat tgcctttttc
     1141 catgaaagtc tcttccgtaa gatggcgtaa attaataaaa gaggaattgc cagtgcaaaa
     1201 tataacccct gatattttat accgactgta tatccgcaag ccgcggcggc aagcgaaaaa
     1261 tatttcccga aacggaattc atccagatct aaaaataaga ataccaaact gatgaaaaac
     1321 aacgacagac tatccggatg ccaccagaaa ttattcaaca aaaccgccgg aatcgtcagc
     1381 agaaaaacaa acaagctcag actgattaat cggttcttaa atcgagttat cagccaagta
     1441 aatatcccga tcgcaaaaat cattggtaat acgttaatcg tcattcgcaa ccagcaaaca
     1501 ataaggcggg tgttctcggg ccaattcgga ctcaacaatt ttaagggaaa gacggatatt
     1561 gccgagagga aataaaatgg gaatccgtaa aaataatgct gataaatgaa aaaattacgg
     1621 atcgagtgat aaagagtatc accgggagtt aacatctgaa tgacatgcgg atattgggca
     1681 tattcatcca cctcaaatac cgacaacatt tccgggtctg ctgctccggt cagatttgcg
     1741 ggaatcagta aacaaaaata gaccaatccg atcagaatga tgaatgcaca ggtgatacgc
     1801 tgattttttt ccatagctgg tactaattat agacgaatga attttgaaat ttttgaatat
     1861 tcaaaataaa tttggaaatt attaaataac gggatcagaa atgaaatcat tcccataagg
     1921 aatagacacg atcgacatac tcgactgcct tctcacaacc gtatccgaaa tagtttttca
     1981 cagtatccca aatcaaaggg tatccgtaaa gataggtgct cgtgattgcc ctttggacac
     2041 atccggatcc ggcataataa ttggcgaccg caattttcca taaactctcg taatccgtaa
     2101 tttcttctaa cggaaattcg gaagtattca gaattatttg ttcaatttgg ctggcacttg
     2161 ccttgacagc cgcggtaatc agatcaattt cctcggcaga atttgtttta gcgatcaggc
     2221 taccaatcaa cacattttga tcttttgttt ccatttcgga gaagcagctg ccacagcgat
     2281 tgggttgcat tttaaagatt gcatagcaga gttgattgaa atatgccgga taccaatcca
     2341 ataacatttt tatccccagt attgtgattc gccccaatcc atattcccct tcgatatctg
     2401 attccggcca aaactgagat tcttgagcta ccattccttt taccagtcgg ggaggaacat
     2461 tatatttctt tccggtctca tagaaccgat cattctgctt attttgccat tcaaataatt
     2521 ttccggaaca agactcaaga ccacaggcat ttgctgattc tcctcccatt aatccaaagc
     2581 ccggacaacg tgacgtatca accagtccgt gccaaatcat tttgccgcaa agaatgtcat
     2641 atggtttatt tgtaatcagg tcgtctttcg aatccggctt tataagcatc ttggaaaccg
     2701 gtttataact cgctggaaat aagccccata gatctgatcc gggaggaata aaatccggat
     2761 aagaacggct taatagatca aaatgatact ttgtcggatc cgatcgatcc ggtaaaaccc
     2821 gatatttcac ccggtgatga acactttgca ctccgttcga gttcattgtc cagtaataaa
     2881 gccattcccc ttgttcaccg gtcatacgaa gcggtaccct gcattcggaa ctttcgcatt
     2941 ctccttcgaa agttcccatc cgaatccgga tctttatcgg gaaagatgta aaagcggatg
     3001 aatcgaccat tcgaatttga atttccggta tttgttgaca gagttgactt ggcagacaat
     3061 cgaccagttc aaatcggagc ggtgggaaag acccaatgga atcctgggat ttttcgtcgg
     3121 gaatttcatt ttgtgaaacg atcgatccgg ttcttcgaag gaacagaccg gtgcaattct
     3181 gattttcgat gatatgatca catggcggag tcgtttgcca ttcgaaaagg atttcgggac
     3241 cgcactgttc taaaatttcc tgttctccgg gctgaatttc ggaattgatc atcaatgtac
     3301 aaaccgttag agaggtattc caagaggcaa gttgccaaac gatttgttga tcggatgatg
     3361 ctgcaaatat agagattgaa gtacaaaaaa tcaggaagat aaagaacgaa acagaaagaa
     3421 gaagaaagaa ggatatcctt ttctttcctc tcataaaagg tcactcgttc ttcgttacga
     3481 gatcatttga ttttagaagc atgattctat ttttgatcta tctatattta tgatatgaaa
     3541 tgatcgatcc gataaaacct ggaaaattat actcggaacg tacttgaatg aacagggaaa
     3601 tggaatttaa tgcggcaagt taaagaataa tagaaaatac cagaataaaa agactttttc
     3661 tgaagaatat tcgataagat tgataagcaa agttcccgtt gaagacttga gaatcagact
     3721 aatattcttc gatattaatc ttttgtatct ttccgataga aaaaggcatg atttgattgg
     3781 caaattcagt cattcttgca gccggatcgc ttacataaaa caaataattt gctttttctt
     3841 gagagaaaga agtatttaga aggttatttt tcgtaagaat ctgccgaagc atttcggcgg
     3901 tgttcaaacc gggatctaca caaattacat tctctcccat aaatctctca atggctgtct
     3961 taattaacgg ataatgcgta catcctaaca gtagggtatc gatattttct ctcttaattt
     4021 ccgttaggta acgggcaata atctgatcgg taagatcatc ctcaaaaagt ccttcttcaa
     4081 cgaatgggac aaataaagga caagcttgtt tataaatttc aaaatccgga ttaatctggc
     4141 ttaaaaagga ttcgtatgca ccggaatgta tcgttgcata ggtaccgata ataccgattt
     4201 tcccatttcg ggttgccgct gccgctgcat aagcacccgg ttcgatgaca ccgatgatcg
     4261 gtatttcggt ttccttccga atatcatcta atgcataagc gcttgctgtg ttgcaggcga
     4321 taacgattgc ctttacgttt tgactttcca gaaatcggac gatctgtcga gtgtaatgaa
     4381 taatagtctc tttggatttg ttcccgtatg gaacccgagc ggtgtcccca aaataaatga
     4441 tattttcttc cggcatccct tgaatcagtt ttcgcatgac ggtcaatccg ccgacccctg
     4501 aatcaaaaat tccgatcgga gcacttttat tcattcaatt tctctccttt tcccacttgc
     4561 aaaaagcttt ttcgagcaat ttacaaatta tacgcggaaa acttgattgc caaatttcta
     4621 ataacattat cattaatgat gtaattcaat cagttataat ttttatatcg cacatgattt
     4681 tgtgtagcga tagtagaaag gttgatgtat cggcgtaaac cggtaaaaat taatggaacc
     4741 tggaagttgt actccatacg aattcaatac ccattatctg acattcttaa ttgttttttt
     4801 atttctgtat tttttgttta cagttattta ttccgtattc agatttaata ggcgccccga
     4861 cattttttca gaagaaaatg aaaaagagaa ggatacggaa aatgcttccg ctttatctcc
     4921 cgagcaagtg aatgttatct ggttggctgc aaaaataaat gcggtcatcg tatccatttt
     4981 tgcgtttatt ctgattaacg acagcttctg cctctatcga agtcaatggc tcttattatt
     5041 gagtatcatt ctgattccgc ttgtattgta tttcttggaa gcattgatca gcggtattgc
     5101 cgcacggaat gccgattcat ggataaagta cttccatggt attgcccgtc tgacagtttt
     5161 tttctttaaa ccgctttatt cggtttatga gtcttttaag cccgataata ttcgcaacga
     5221 ttatactttc aatgaagtgg aagcccattt aagagaatgg gtaaataatg ctccggataa
     5281 tgccgctttg aaagaagatg aacgaaaaat ggtccgttct atccttcatt tcagcgatac
     5341 tctgattcga gagataatga ttcctcgtat tgatatgacc gcggtagatg tcgaaaccag
     5401 tcttgaagaa gcgacatcca ttgtattggc ttccggacat tctcgtttgc cggtttatga
     5461 ggatgatatc gataatatta tcggtgttct ttatgcaaaa gatctattaa aaatctatac
     5521 ggaaaatccg gaaaaccgcg cgctgcggga ctatctgagg cctgctttat ttgtgcccga
     5581 atccaaaaag gcaggtgact tattgagtga aatgcaaact tccggaattc acatggtgat
     5641 tgttgtcgat gaatacggag gaactgccgg gatcgtatct atggaagaca ttgtagaaga
     5701 aattgtcgga gaaattcgtg acgaatatga tgacagcgaa gaaaagctga tcaatgaaat
     5761 cagtgaaaac gaatattctc ttttgggtag aatcgacttg gaagatgtga atgaaattct
     5821 cgggacccat attacgcgag aatccgcgga tacacttgcc ggcttccttt actcccagat
     5881 cggtaaagta ccggtaggtg atgaggaaat cgaagtggaa ggttggatat tccgcattga
     5941 agagttatcc ggaaacagaa tcaggcgtgt ccatgtaaaa aaattggaag aagattcgcc
     6001 gaacaaggaa ggtgaacagg atgaagagtg aaaatgatct tcgggaattg attgatatcg
     6061 cattggcagt ccgcggtaaa gcttatgcgc cctattcgaa atacgcggtg ggcgctgcac
     6121 tgcgaacagg cagcggtaaa atctacaccg gtgtgaatgt ggaaaactct tcatatcctc
     6181 tcactatttg cgcggaacga aacgccatct tccatgcgat tactgagggc gattcggatt
     6241 ttgaaacgat cgtggtagcg accgagaatg gcggatctcc ttgcggtgcc tgtcggcagg
     6301 taatggcgga atttgcgttg gatatgaacg ttgtgatggt ggataaaagc ggaaagatca
     6361 ttttacaggc cactgtggat gaacttttac cggaagcttt cactccgaaa tcactcagaa
     6421 aatagcacgt tatggaaaga tctgttcgag ttcaggctct ggttctcagc cataatgact
     6481 ttggtgaagc ggatcgatat gtaaaattat tgacacccga tcgaggaaaa atttcggcct
     6541 tggcaaaagg agtgcgaaag atgaattccc gaaaagccgg tcatttagag ccgtttacta
     6601 taatttcagc ccagctggtc caagggaaag gatcatcttg gattatcagc caagtttcta
     6661 ccatagagag tttccccggc ttgaccgata atttggaatc gacttgccat gcggcttatg
     6721 tctcggaatt gacggatcgc tttgctacgg aggaatattc gaattttgag ctttataaac
     6781 tggctgtgga aaccatgcgc cgtctttcaa aaataaccga tcatttcccg gtgctgcgat
     6841 atttcgactt cagactgctt gatttggccg gctttcgacc gcaacttcat tcctgtgtgc
     6901 ggtgcggtaa cgaaattatc gaagagcaac aatttttttc aaacattttg ggcggagtgg
     6961 tttgtccgaa atgcggtatg aaagaatccg gcatcgtttc catttcgtct cgctcgttga
     7021 aatattttcg ctattatcaa acacacaact tttctcaagc tgcggcagcc ggctggcccg
     7081 ctgatctaag gcaagaatcg gaaatcatcc tgaccaatta tctgacctat tttgcggaaa
     7141 aaaagatcaa tagtcagatc tttttaaact gtattcgttg atcattgatc gataaaaata
     7201 tgagaatcac tttataaacg gaggtgaaga tgcgttacgg gactttcgac gacgagaagc
     7261 ttgaatattg tattacgcaa ccggatacac cgctgccttg gattaattat ttggggacag
     7321 aagatttttt tggattgatt tcaaatacgg ccggtggata ctgcttttat cgggatgcac
     7381 gtttacgtcg actgacccgt taccgctata ataatgttcc tgcggatagc ggaggaagat
     7441 atctttatct gcgggatcga caaacaggcg actattggtc gccgacttgg caaccggttc
     7501 gatcaacttt ggatcactac agctgccggc acggattggg ttataccgtt atcgcttcag
     7561 agaaaaacgg aattggcttt tcgggatgct attttattcc gatcggtgaa aatctcgaaa
     7621 tctgggacat tacgatcaca aatcggcgaa aagaagaagc ggatttatct ttattttcat
     7681 ttatagaatt ctgcttatgg gatgcacaag atgatgctac caatttccag cgtaattaca
     7741 atatcgcgga agtggaagtt gaagggagta cgattttcca taaaaccgaa tatcgagaac
     7801 ggcggaatca ttttgcattt ttccactgtt cggatccgat agcaggtttt gacacgcaac
     7861 gagaagcgtt tctcggacgc tatcgtggct ttgatcaacc tcaagcggtt gaacaaggag
     7921 tttctttcaa ttcgattgcg acgtcctggc agccgatcgg ctctcaccaa gtacaattcc
     7981 atctcagcac cggtgaaagc cgccggatca tttttttgct gggatatcat gaaaatcccc
     8041 gaggtcaaaa atttgatcct cccgaatcga atacgctcaa taagaaaacc gtttatccca
     8101 ttattcgtga atgggaacat ccggagcggg ttgatcgggc attttcggac cttaaatcct
     8161 attggaaagg tattctgcaa aagttttctg tccggacacc ggtgaaccat gtagatcgta
     8221 tgataaatac ctggaatgca tatcagtgta tgatcacatt caacctgtct cgctcggcat
     8281 cctattttga aagcgggatc gggcggggaa tgggttttcg tgattcaaat caagatctat
     8341 tgggatttat gcatatggat cctgaccgcg ctcggcaacg gatacttgat cttgccgcga
     8401 cgcaaatgga aaacggaggg gcatatcatc aatatcaacc gcttaccaag cgaggaaata
     8461 atgatatcgg atcaggcttt aatgacgatc cattatggtt agtattgagt acatccgcat
     8521 acttacgcga aaccggtgat tattctatat tggaagaatc cataccgttt gagaaccgag
     8581 ccggatcgga acaacctctg tcggagcatt tagaccgctg catttcttat acgatagaac
     8641 ggataggtcc ccacggactc cctcttattg gcagagcgga ttggaatgac tgtttgaatc
     8701 tgaattgctt ttcagattcg cccgggcaat cttttcagac caccaccaat cgggaaggta
     8761 aaaatgccga atcggtattt attgccggtt tatttgtttt ggctgccaat gcttatgcgg
     8821 atattttgga tatattacaa caaacagaaa aatcatctcg gattcgtagt tatgcgaatg
     8881 agatgaaaaa agttgcggat acggcgggat gggatggtcg gtggttttta cgggcgtatg
     8941 acgatcaagg cgctgcagtc ggctctcatc gttgtaccga aggaaaaatc tttatcgaac
     9001 cccaagggat ctgcactatg gcaggcatcg gtctcgataa tggtcatgcc gcgcaagctt
     9061 tggattcttt gaatacatat ctatccggga aagacggaat tgcattatta tggccggctt
     9121 atactcatta ctatatcaat ctcggcgaaa tatcatcata tcctcccgga tacaaagaga
     9181 acggatctgt tttttgtcac acaaatccat ggataatgat tgccgaaacg aaactgggga
     9241 atggggatcg ggctcttgat tattatttga aaatttgccc ttcttttcgt gaggagatca
     9301 gtgaaattca tcgttgtgaa ccctatgtct atgcccaaac gattgccgga cccgaatcac
     9361 ccactcccgg cgaagcgaag aattcctggc tgaccggcac cgcggcttgg aattatgtgg
     9421 cgatcaccca atatatcttg ggaattcgac ccgaattgac cggattacga gtggaaccga
     9481 ttatcccaaa agattgggaa ggctttcaag ttgttcgaga ttttcgcggc gtccgatatg
     9541 atatatccgt gaaacggaag ggaaaaggga atcattgtca attgaatatt gacggacagg
     9601 atatcgaagg gactgttatt ctttcttcca tagtgaaagg aaaaaaagaa gtaacggttc
     9661 aagcgatcat tcaatagaaa gaaagctccg aaaataaatc ggagccttct gttttatgag
     9721 caacaaaaac tctaattact tgatttccca cttttcccaa tgtgcataac cggttaacgt
     9781 ttcgggagcg atgatattcg gtttgcgcag aaccaaactg ctataccagt aaatcgggtt
     9841 aataatcgcc tcttcttcga taataatttc atcggaacgg gcgtataaag cagcgcgggc
     9901 tttcggatcg gtctcttttc cggctttctt cacaacttct tcatactcga tataagccgg
     9961 attggattta tccgtacaat cttttgacgg ccaatctgtt acagaagaat aacctgtgcc
    10021 gcataaaaag acatcggcgg tgaaattgtt ggcatccata taatcttgaa cccaggtgct
    10081 gcgataaaca ttatccaaac cctgttgccg ttcaacctta tagacagccc attcactatt
    10141 ctttaagttt acagtaatcc ctaaggtatt ttgccacata tattggattg tttcagccag
    10201 cattttcctg gattcactgg tattgaaatt gtaagtgaca acgatatctt tcggttgaat
    10261 ccctttttcc ttgcaatatt cgtcaatcaa tgctttcgcc gcttccggat catacataat
    10321 gcccaaatca gggtaatctt caactttcgg tgccccgtcc actcccgggt ggatataaaa
    10381 cggtgctgct attccctctt tcaatgtctt gactacagct tctttgtcta ccgcaaggct
    10441 taacgctttt cggattcgaa cgtcatcggt cggcgggagc cattcgttgt aaataagaaa
    10501 ctcggttccg accgaatccg tcgaagattt gatctgatcc ttgaaagccg ggtcagaagt
    10561 aatccgatca taatcggcac tgggaattcc ggcttcatcc atattcccgg cttcgtattc
    10621 cgctaaggca gcggaagtat cgatcagaga atagtgaaca cccgcgatct tcggtttggg
    10681 aacaaattcc gttcccggcc aaaaaggatt ggcgataatt gtcaaatggg agtcgtgaac
    10741 ccattcttcc agtgtaaacg ggccataccc ttggtaaaaa ccggtttctg tccaacgttc
    10801 ccccaaagct tcattacagg catctccgtc tataatccat gaaggcatgg catgtaacat
    10861 ccacatactc aggatgttaa gattaaaaac gcccgcttcc ttgaaggtat attctatggt
    10921 gaaatcatcc aaagctttaa tacccacggt tgagaaatcc ggccgggcta ccggcgtacc
    10981 ttccccttct tcgaccggcg tattataagc ttccgcacct tctatcagat tggcaacata
    11041 ggcatatccc gatgctgttt ccggccgcaa tgttctttcc gcaccataga caaaatcatg
    11101 tgcggtcaca tagcgcacat ttccgtcgca atctttgact tcttccactg cttttgtctc
    11161 gggattataa cggacccagg gaacattctg aagcaattta aatgtatata ctctgccgtc
    11221 atccgaaacc gaatattcgg ttgccatgcc cggttccaat aatcccgtgg tttcattttg
    11281 tctcatgatg ccgactgtca tttcatcaac caattgaatt cctatgaaat cggttaccag
    11341 agcctggtca attgaaggta tatctccgct gccaaagtat gttcgaaaat actttgcttc
    11401 atcagcgagg acaggcattg ctatcagcat gagggaaatc aatgtaatgc tgaataataa
    11461 aagcaatttt ctgttaatca taatcacacc tttctttgtt cctatataaa gttcataccg
    11521 aatttcgata tgtcattaaa aaatggtaaa aactcttttg aaaataaaac gataaatacg
    11581 atattaatct aagaacggtt atttttcaag gttttcgtca taatttgtta ttgagaaata
    11641 aaaagattac ttgtgctatt ttccgataga tttagtaaaa gacaaaaata tcctttcgat
    11701 caggagtgct tttatcgtga aatatagtga aaattcttcg caacccattt ctgacagtat
    11761 ctcatccgtt attgtatccg gctcgaataa atcctgtaaa atctcgccga ttttagcggt
    11821 cagttttgga tcgttgttta ccggcggcat aagttaattg atcggtcgct ctggctgact
    11881 gtgtaatacc gcttattttc tgaagcatat tcattcatcg gcaatgcatc cagacagact
    11941 cttattccca gtgccggccg cgcttctttc cctcagagga gtttgaccac tccggttcct
    12001 ccgataccgg tttgaacttc gcgtcccaaa ttcttcatat atcctgcggc tttttccgct
    12061 gtccaatatt ccttgaaacc caactcaaga aaacggtgaa aatccttcct catccgtacc
    12121 agttcatctt ttatttcaat tattttttgg tccaaatccg caagtccgta agccgcttaa
    12181 actcctttca taaagtcatt attcaatttt tgatatgctt tcttgtcttt gtattcctat
    12241 caagaaagca tcttatgaat ttttgtttga gatgatatta ctgctgaata acgacacata
    12301 aagatgtgcc ttccttgacg atttcctctt ccaggtgtat attaaaacag cctgaaaaaa
    12361 ggttactgcc ctcatagctc aattggacag agcgtttgct tgcggagcaa aaggttgggg
    12421 attcgacttc ctctgggggc gctcacttat tttttttcaa aaatttgaac attttttgat
    12481 gcctttaaaa aggcatcgtt taattaaaaa gaggcgacaa tttaataaac atcgatccga
    12541 tgaggaatcc atgtttattc atttctaaaa caaaataaat aaaataacat ccgttgtcag
    12601 aaccgattaa tctgagatca ttgttcgaaa tttgatgatc attccgaaca atgatcttac
    12661 cggctctatt tataatgggt tgcgcggaaa aacctcatgc cgcccggata aactttcaca
    12721 ttttgaaagc cgttagccat taggattctt gcagcgttat aagcacggac acccattgca
    12781 caaaaaataa tgatttcttt ctctttgtca atttcagaca gtcttgatcg gatctgtccc
    12841 aatggaatat tcaaagcccc cgacaaagca taagttcgtc gttctgcatc ttcgcgcaca
    12901 tccaacaata tggttttttc cggatcatga cgttccacaa catcccattc ggcaaaaaag
    12961 actctggatt tgagaatatt ttccgcgaca aatcctgcca tattgaccgg gtctttcgca
    13021 gaagaaaaag gaggtgcata agcgaattca agtgatttca gatcccaaat tgttccgccg
    13081 agacgaatag cggtgccgat ggtatcgatt cgtttatcca ctccttcttt cccgataatc
    13141 tgggctccga agattttatt tccgtctgtt gaaaaaagca gtttaatagc catcggagag
    13201 gcacccggat agtatccggc atgagaattt tgcagaatga ttaatttttc ataatccttt
    13261 cctttgatca ggccattttt taataatgtt ttttcattcg cgcccgtatt tgcggcacat
    13321 acatcaaaga tcttgacaat cgccgatcct tgggttcccg ggtattgatc gttttctccg
    13381 gcgatgttac cggcagcgat gcgtccctgt ttgttagccg gtccggctaa cggaatcata
    13441 gttttgcctt tactaataaa atcttcgact tcaatcacat cgccgaccgc aaaaatgtcg
    13501 ggatcagatg ttcgtagtgt tttatcaaca acaattccgc cgcgggcatt taacgctaag
    13561 cccgctgaac gagcaagctc gctgtttggt ttgacgccga tggataaaat tacgagtcgg
    13621 gtagctaatt gcttgccatc ggatagatta accagaatgg attcatcagt ttccgtaaaa
    13681 gaggtgacgc cgtttcccaa ataaagcgac acaccatttt cttccaatgt tttatgaatt
    13741 aactgtgcca tttcaaaatc aaacggagcc attacctggt ttaacatttc aaccaaagat
    13801 acagtaattc caagccggcg caggttttcg gtcatttcga tgccgatgaa cccacctccg
    13861 attattatgg catcgctgat tttattttgc cggatatatt gacggatgtg atccgcatca
    13921 tcaacggtcc agagtgaaaa aatatacttt gatcggatac ccgggatatt cggtattatc
    13981 ggtgaagacc ccgtagccag gaccaatgta tcataacttt caaccgattc ttgagaagtg
    14041 gctaaatttt taacagagac ggttttgttt gcacgatcaa taaaagttac ttcctgctga
    14101 atacgaacat ctatgttgaa tttggatttc aaagattccg gggtctgcaa cagaaggtcc
    14161 tcccgcgatg ggattacatc tcccacatga tatggtaaac cgcaattggc ataagaaata
    14221 tatttcccgc gttcgaaaag gatgatctcc gcattttcgt ccagtctgcg caacctggct
    14281 gcggttgtag cgccccccgc aactccgcct acaatgatga ctttcttgtt catgaaagtt
    14341 gcctcctgat taaggatttt gaaatccgat tttcatttat cttaacatat ctgcaaacaa
    14401 cttacgatat ttttccatat ttcggattat acaaatgggc ggtccgaaag attgatcatt
    14461 gggctttatg aaaatcgatc gataaagttc atatattcac tgcgactttt atttaaaaaa
    14521 attgccgaat tttataagag cagcaaactc tttgaaatga aattctttct tgcgatgaaa
    14581 atgcttcaac gaagtgcttt ttaataaatt aaatgaaatt tgatcggtct tatcaaaaaa
    14641 attataaaaa cggtctatgg aataaaaaaa ttgaactttt gaggaaggat atatttcatt
    14701 tctgtctcat aaatctgatc tgccgatcat ttattgtaaa tttaatgaat aggaatccat
    14761 tcggaatgaa ttccgttttt tagatttaaa gaaaagcgtg ctgcaaaatt acgcacactt
    14821 ttctttctga ttttcgggtt tatccatcat atattgttta aaatcaattt aaagaagttt
    14881 cgattccgtc tttagacagc tcctgtttcc ttatttgttt tctcccgttt taactttctt
    14941 ttttctttaa ttgtcatttt cggcttcttt ttttgctctt ttttagcctt atcatttttt
    15001 cctgtatcag ccataacaac ctcctatgtt tttcttgaac cggtttacga agaaatgaat
    15061 atcattcaat cattttttct atactatttg aaattgattt acttgtcaac aaaccagatg
    15121 tatatttcct cggttattta atccggtcta taatattttt ttataggaat cggaccagta
    15181 taagtgaagc aaaccgaaga aaatgattaa gcattttggc tcctttctct cagcaagtat
    15241 ctgacggtta aacaatcaga gcttcttccg attctcagat tttcctgaat ttgtttgtta
    15301 ataaattata tcgaaaaaaa cagacctttc aacatttatt tttgttcggc attaatcaac
    15361 ttgacaattc tttccgcggc ttttccgtca ccataagggt ttgtggcttt ggacattttt
    15421 tcccattgat cggggtgatc cagcagatcg catacgtgcg tataaatatt ttcgggatca
    15481 gtccccacca atcgtacatt tcccgagatc acgccttccg gtcgttcggt cacttcccga
    15541 agtaccagta ccggcacacc catccccgga gcctcttcct gtaaccctcc cgaatcagtg
    15601 agtaatatct tgctgttttt caatagatac accatcgaaa gataatccaa aggcggaatc
    15661 aagctgatat tcggttgatg atccaaaatc gaaaaagccg ttttgcggac gatcggattc
    15721 aggtggaccg ggtaaacaat atgaacacga tctgcataaa cttgactgat ttttaagatt
    15781 gcattacata tatcgactaa cggctttcca tggttttccc ttcgatgtgc cgtgacggtt
    15841 ataatttctt tttctccgaa agggatccct tccaaaggag tacctttttc ttcaaacggg
    15901 atttttaatg ccagttgctg agcgtcaatt accgtatttc cggtaacatg gcagatttcc
    15961 ggaggtattc cctcacggag cagattttgg cgtgccattt cagtaggggc gaagtgcaaa
    16021 tcagcgatga cgccggcgat tttccggttg atttcttccg gaaaaggcgc ccatttattc
    16081 cagcttcgaa ggccggcttc cacgtgaccg actttcattt gattataata ggcagccaag
    16141 gctgcggcca tcacggttgt cgtatccccc tgaaccagta tccaatccgg ttttctttcc
    16201 agaagtactt tattcatttg tatgagaatc gaagctgtga tatccgatag tgtctgattt
    16261 tctgtcatca ggttcaaatc gatatccgga ggaatgctga aaatctgaag cacttgatca
    16321 agcatttctc gatgttgagc cgtgaggcag acgatcgttt cgatatgcga ttccgcttga
    16381 agacatttaa tgacgggtgc cattttaatc gcttccggac gggtgcctac taccaccatc
    16441 actttcagcc tgttgttcat aatggttatt tgaccatcct gttctttttt tcaatcttat
    16501 cagttgatcc gaattgctaa atgaaatgtt atcatgattt aagttttaag agaaagataa
    16561 aaagaccggt ttggttgaga aataatatcg ttgagcgaat ctcatctatg gtataaagag
    16621 agacagttta aagatttaca tatttttggg ggagtcccta atgctggcaa aagtatattc
    16681 ctgtacagtt acagatctgg atggcgatat tatcgaagtt gaagtcgata attcttctta
    16741 tggtttacct cagacagtaa ttgtaggatt agcggatacg gcggttcagg aaagcaggga
    16801 gcgggtaaga tctgcgatcc gaaacacagg cttggaatat ccgcggaaaa aggtcaccat
    16861 taatctcgct cccgcagatt tgagaaaaga aggacccttc tttgatctgc cgatcgcgat
    16921 cggaatttta gccgccaatc gcattattcc attaaccgct tttgaaaaca gcttgattat
    16981 cggtgaactg tctttggatg gctcggttcg gcatgtccgc ggtatcttac cgatcacagc
    17041 caaagctaaa gaatccggat tttcccggat ttatgttccg gcttgtgacg ctcgggaagc
    17101 ttccttaatt cccgatatcg atgtttttcc ggtaacatct ttgtctgttt tgtatcatca
    17161 ttttataggg acagaacaaa tacagccggc tgaaccgatg aaaattacac aggcgattcc
    17221 ggaggtatta accgatttta aagacattca agggcaggag catgtcaaac gggcgctgga
    17281 aatagcggcc gccggttctc ataatatttt aatggtaggt cctcccggaa ccggaaaaac
    17341 tctgatggct cgggctctgc cttccatctt gccggcctta accgttcagg aagctttgga
    17401 cgttacccgg atttattcta tcgccgatca attgccttcc gaccggcctt tgatccaaag
    17461 ccggccgttc cgttccccgc atcataccat cagtaatgtc ggattggtag gcggcgggaa
    17521 tcacccgaga ccgggagaga tttcgatggc acatcatggt gttctttttt tagacgaatt
    17581 tccggaattc ggccagcgcg ttttggaagt tttacggcaa ccgttggaag acaaagttgt
    17641 taccatttcg cgggcttcgg ggtccgtaac ttttccggct aattttatgt tggtggctgc
    17701 gatgaatccg tgcccatgcg ggttttatgg agatccggtc catccctgtc attgttctaa
    17761 tttggcgatt tcaaactatc aaaaacgaat ttccggtccg ttgatggatc gaatcgatat
    17821 tcatgtagat gtccctcgaa ttgaatttca aaaaatcagc agtcaaaaag atcaggaacc
    17881 atcggcggcg attcgggcac gggtagagaa agctcgtgaa attcagcggc aacgtttttc
    17941 ggaaagcaag aatatcagtt gcaacgccga aatgcgcccg gctgaaataa aaaagttttg
    18001 ccaaccggat gatgaaggga accggctgat gcggctggca atggatcggt atgaactatc
    18061 cgctcgggtg tttcatcgat tattaaagtt agcaaggact atagcggacc ttgatggatc
    18121 ggaaaaaatt caatccaacc atattgcgga agcgattcaa tatcgtcccc ggcagactga
    18181 ataggttgga gaataaattt gtcattataa aaatcattga tacggaattc caaacattaa
    18241 tatgatttta gaatttcttc ataggtcggg aaccccgg
//
LOCUS       DAOSMM010000047        17871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3180_length_17871_cov_13.870678, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000047 DAOSMM010000000
VERSION     DAOSMM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 17871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17871
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3180_length_17871_cov_13.870678"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PK898_07445"
     CDS             complement(<1..795)
                     /locus_tag="PK898_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU19904.1"
                     /translation="MLLKEKVALITGAGRGWGRSIAIAYARQGASVIAVARTKSELDQ
                     TAEIIRQEGGNVTEFAFDISEENAIKEIFQYISDHFSQLDILINNAAVLMLKPFTDLS
                     LDEIDQIIKINLRSQIVLCKMAIPQMIKQGGGSIINVSSGAGVKGFEKESIYCATKHA
                     IEGFSKSIALELMPYNIAVNTITPGGKSQNIQIKPTSLTQSEFELLSAEEKSKWTDSI
                     VSTDAFVFLGMQRADGITGQRILAYEFSQHLHECAWQLGLADLRDGL"
     gene            complement(826..1770)
                     /locus_tag="PK898_07450"
     CDS             complement(826..1770)
                     /locus_tag="PK898_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU19905.1"
                     /translation="MEVYPRNKRNENAYIQNDKPLVVKVPHFNGDYLVDTVYTAIETL
                     GGLNKKFRSGDSVIVKPNFNCPDPTPLSTGLDFLAAVFEILTDFGLHIKVGEMCGRAA
                     WPTEEVVQKLKVLPVMKRYGVDFINFEKDDWIPVEISGDFWKVIHVPRTIYEATHRVY
                     LPNMRCHSSARFSGAIKLGVGWLSPEDREIMHCDKDKTEQMIAEINLAFQPDIIIMDG
                     RRSTVGWAGRGDYVYPNVVMASGDMVAIDTEAIKILKQYPSRNRIQIPIDEMKTLTTA
                     QKHGLGKMDYQIIEMEPHLSTREKNNNDPAAIAVMNDL"
     gene            complement(1901..2920)
                     /locus_tag="PK898_07455"
     CDS             complement(1901..2920)
                     /locus_tag="PK898_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU19906.1"
                     /translation="MVEAIKIGNISVMPGEMKRGAIAIGKNMYGIERELPVIVYRGLH
                     EGKCLWITGSTHGDEPEGPFSIKLLMDRPELDPAKMSGTLVLVPALNVEALMDGRRGD
                     PRDAFSYDMNRFYPGKPDGYPSERVAWAFHEIASKYADLHLAIHSGGDHSMLCRAFFA
                     PENPESLELGKALGPEWDLALTSNVGGTGNPTSVFAGMGKGALTVEHGGYCRCLTTEF
                     VEVCMKYVNGYINIMKHYHMIEGESVYADKWYRGHQIALLANASGLFVGADVPLREMI
                     KKGTVLGHIYNLYGDVLETLTAPADGQVFGLRYRPSVIEGEWCCFFGAIDEIRNDLMI
                     AGKSK"
     gene            complement(3018..4025)
                     /locus_tag="PK898_07460"
     CDS             complement(3018..4025)
                     /locus_tag="PK898_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU19907.1"
                     /translation="MTKVVIADSGTKEQIANWHDFGIRYELDVVMPSTPDEEELFRLL
                     PDAEVLVTQKVTIDERMMNAAPKLRLIQKMGSRADNIDLEAAKRRRINVSVVSLPGSV
                     AVAEHAMMLILACAKKLILAHELTVKGAYRDLGIEPKLTSQKSHGFQWMKIPDLQELN
                     GSTLGIFGFGDIGNEIAKRAHAFEMEILYFKRHPLPEKMEEELGVKYVSKEELFKRSD
                     FLVMISPLTPETEKAAGAKEFALMKPTAYFINCCRGGVVDEEALVETLKNHRIAGAGL
                     DVFVYEPIPYDHPLLSLDNVTLTPHIAGGKGNAAERQIYTVLENIQKYLKNEPLKNLI
                     C"
     gene            complement(4082..4786)
                     /locus_tag="PK898_07465"
     CDS             complement(4082..4786)
                     /locus_tag="PK898_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOU19908.1"
                     /translation="MKILVLNPNTSEAVTQRIAETAKKAAYPDTEIVVTGIPHGPESL
                     ESFYDEAIATPYILQSVKNANEAGYDAVINAAFCDPGIEALKEISDIPVFGLEECSFS
                     AALLFGNKFAILTEQKHKEAVKSREVRKHGLESRFAGVKALNMGVVEIANNPLKVKEA
                     GIKAARELIENGAEVIILGCSSMAGYSEDLEKELKVPIIDPVATTVKMAEAFTALGLR
                     HAKIGLYSKPKIQKMK"
     gene            complement(4865..5758)
                     /locus_tag="PK898_07470"
     CDS             complement(4865..5758)
                     /locus_tag="PK898_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU19909.1"
                     /translation="MILRAHHIGIAVEKLDQAISTYDKLFGFKATDIRNNQGKGFQLD
                     ARIMMPNRTWLHLVENWNPESRVNKFLQQHGEGLEHIALETTDIEEEVAYLRSIGVPV
                     FEDKIFNAADGFEAFIYPDAAIGFTVELIQPHITSYYWQPEKVSNPNLLGLQHIGVAV
                     KNVVEAANKFEKLFRIKSSDLRTDQHYGTQKDIMIEPGNDRLWLHLVESTDPENRVTQ
                     FMAQHGEGLEHLAFEVNDIRRAVKTVKKAGVSLTANKIYLDREDGFEAFVDSKNNHGV
                     TVELIEPYITSRGYRYKVEDF"
     gene            complement(5900..7447)
                     /locus_tag="PK898_07475"
     CDS             complement(5900..7447)
                     /locus_tag="PK898_07475"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOU19910.1"
                     /translation="MCFMIAVGKKASADGSTMVARNCDSNSTEAQRIIAYPRKKHQPH
                     ELIRIPDSNNVEIPQVEETFAFIAITRYVDGDDIGMVAGGVNEFQLSAGASTGGWLKK
                     EVEDISPMPETVIGDYRMQLILERCKTAREAIEWLGQNVAEYGARTDNYIIADPNEVW
                     LYEEYQDHHWAAVRVPDDSFVVEGNSFRIADFFPEDTANYLCDPDLIPFAIQHGLWDP
                     NSGKPFNVSRAYGTNNRNRPRGKHGEFEQPYYSLHRIWRSISLLNTDLNLDEYEPTQE
                     YPLFVKPSKSITPEMLIHVLKDTYEGTDLDEYGKTDMPYGTIVDPVTGHYRYSPAWSK
                     SRIIGCPQTITSWVTQSRSWMPNEIGGLIWAGLAATASSPHIPFYACHKDTPSAYKIG
                     IAGDNSVYLPDSAYWLFENIGNIMNLFYDGTVDLVKPVWEDFDHRRYEFQNSIEQIAL
                     NLYKEDKQKMIDFLTSYSNGVALEALEVGQDMLAKLFTRLAMVNNPQTTRGYENPAEW
                     KKSGFIY"
     gene            7904..8809
                     /locus_tag="PK898_07480"
     CDS             7904..8809
                     /locus_tag="PK898_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOU19911.1"
                     /translation="MDIFERIKKEQDGFSKSFKKVADFICSNYEEVAFMSITEVAKRS
                     GVSESVIVRFSAALGYSGFIEMKKNIAEKVKENLKLPNRMTSSSLSENMSANEIMTAV
                     LAKDMENILYTSKEPYNKSFEQIVREIHDAKKVYIIGNRGLYKIAELTELLLTPIGIP
                     CISINAEDSIQFQQLNSMRKNCVLLAFSLPRYSSHTYTAIKIAKKYHCKTIVISDSIF
                     SPDAKAADLSLEITVKSYTYINSYCAIVSMMNAIITAIGVLYKTETLNSLNRLENFLQ
                     NFQVIESYSKNNSESEKRISQSKTS"
     gene            9007..10185
                     /locus_tag="PK898_07485"
     CDS             9007..10185
                     /locus_tag="PK898_07485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU19912.1"
                     /translation="MSCNALDRLRSDKTRTVGELSIRTKLLEKAATLDDVILMGRGDP
                     DLPTPKHIIEAAKKAIDNGATHYTHVRGTIELREAIAEKLKRDNNLDYDPENEITVTV
                     GAEEAVFLSLFGILNPGDEVLVPEPRYNSYDEAILMLGGVPKVVKCIPEENFRLTPER
                     LEANISPKTKAISLVNPGNPVGMYDPEQVKELCQIAIKHDLIVISDEIYENIIFDDTP
                     HLSVASIPGMRERTITINGPSKSYAMTGWRCGFFAAPAPFCEILTEPAHTISICCPAP
                     TQAAALAAYTGPQDCVKEMRDIYKERCNIMATALTEMGFSFIPPRAGFYIFTDVTSLG
                     MTPEEFCMQLLMQAKVLIFPGSLFLDPTGRYVRISLLSPTERIIAAAERMKNFVKQYK
                     "
     gene            10199..11338
                     /locus_tag="PK898_07490"
     CDS             10199..11338
                     /locus_tag="PK898_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOU19913.1"
                     /translation="MKLTTDWYIKKMSQIQSKLSEEKLDAILLLDPYNIFYASGFFHQ
                     STERPLGLLIPQMGEPSLYVPKLEQEMASETWIKSIHVYFDFPGLIHPIAWMLKENSQ
                     YSHIGVDQLNYRQWKIAQEGCPNIQISDLVYTMRLVKDAEEIQILECASVYADYMVEM
                     TKDAIIEGLSELDAYNYARDHTVNKMIKELGELTFANQGTTNGAVLYGAHSAHPHGLM
                     SSTLKPKPGDVIEAGFGALVCTYESESEHTFIYGEPDHQTVSYFNAMYNAWKAGLESA
                     KPGVKCCDVNKAALEEIRRAGYEEFLRHRMGHGKGLQEHEPPWVAEGDMTVLKPGMII
                     SDEPGLYVPGFGGFRHSDTLLITEDGCRRLTKYPRELEACIIPIK"
     gene            complement(11453..13774)
                     /locus_tag="PK898_07495"
     CDS             complement(11453..13774)
                     /locus_tag="PK898_07495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU19914.1"
                     /translation="MENKDNSKMLKPICPESLFDYSIIEDPQFSTDGKKIAFVRMQAD
                     EEGNDYHRTIWMVDTDGKSEPYPFTNGTSDQNPRWSPDGTRLAFISSRDGSSQLYVIS
                     LNGGEAVQITDMVGGVSNINWSPDSKWIAFNSLSTLNEHHLEDLGVLYNKGLKEVPKE
                     WSKMHRQELQDPRVVTQLPYRTGTAFFDGCYQHIYVISSKGGSLMRLSNGEYHHGAPE
                     WSKDSKFVFSNSKRDQSSGEENFELWSSVLKFDIYSAKEEVILSEVSEEGREIEISPD
                     GRWLAYFYVQKAESPYEEPYYVAVNSCVEKAENKIISTEDQTVINFKWDSDSKHIYFL
                     VHDHGDGKIVQNDLAGGTPREIVYGKRMVEQFDVSTDGKMIAFSVSSPITPGDLFIKN
                     VDTGEENQLTHFNKDFETSHAFSIPDELEFTGTDGLSIQGWYFKPKDFNPQKTYPAVV
                     EIHGGPQIMWGNSFWHEFQVLCSRGYFVFFCNPRGSAGYGARFQKLRGKGGYTDMADI
                     MNGLDAFLAKERSVDQNRLCVTGGSYGGFLTAWIVTHTGRFAAAVAQRGVYDELNMFG
                     SGDIPESVEWYYGGVPREENLKELWEYSPIAHAKNVTTPLLILHSELDYRCPISQAES
                     FFTHLRRNGNRDVNMVRFPREGHNLSRNGEPWHRIERLRRITEWFDHQIKYTSLIDEI
                     LTDDQLNQWIYTLKGWRRENLKLIRSTESGNNDIANMMVRKIWNYLKMNRKQCNLIIE
                     GSRLTVEIQGIDSRGITMREILLADRLNKIFFA"
     gene            complement(13807..14730)
                     /locus_tag="PK898_07500"
     CDS             complement(13807..14730)
                     /locus_tag="PK898_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19915.1"
                     /translation="MMEPSNNTTKSEKHAVEIKKPKKSSNSRFLYDLKKSTKAKFAII
                     VIFIVSLFAILAPILTPYDPVTTDLMARLKPPVPFRYSVVSHPLGTDALGRDIWARIV
                     YGARVSLLVGITSVIISGCIGVTLGLIAGYFGGIVDDIIMRIADILLAFPFILLMLAV
                     LAVIGPGLGNMILVLGLTGWVSYARVVRGEVLSLREKEFVESARSLGASNMRIIFKHI
                     LPNVVNSVIVVASFAVASTIITEATLSFLGLGVKPTTPTWGMMLSEGREYIRQAWWLT
                     TLPGLAIGITVLGINLLGDWLRDVFDPRTLL"
     gene            complement(14754..15680)
                     /locus_tag="PK898_07505"
     CDS             complement(14754..15680)
                     /locus_tag="PK898_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19916.1"
                     /translation="MARYTARRLLQAIIVIFGITIAAFSINFLAGDPTYVLIGDMRGM
                     TEEDIQAFRHRMGFDRPILVQYFDWLKNAVRGDFGWSYKYKEDNWKIIVERFPATLQL
                     TVVSLILQLLVSFPLGVFAALKRGTKWDSFSMVLALLGQSMPSFWIGLMLMMIFSVNL
                     KWLPVSGRYEGIRSMVLPVITLSFYGIARNTRLIRSSMLEVLGEDYIRTARAKGQTER
                     NVYIKHALRNVLIPVVTMVGMDLGSLLSGALVVETIFAWPGMGMLTVNAINGKDIMMV
                     QACVTLFALMFVFANLLVDFTYTILDPRVRLQ"
     gene            complement(15854..17407)
                     /locus_tag="PK898_07510"
     CDS             complement(15854..17407)
                     /locus_tag="PK898_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19917.1"
                     /translation="MKKIMFVLLALCLLALPVTVMAQQEVDTEATVTVALTTIIDNLD
                     YMIHSALNASGIFQHMFEPLIDLDPQTVTIVPCLATEWSTSEDGKVWTFKLRDDVKFW
                     DGEPFTAKDVKYTMERMLMDDYNIGNTNYLNNQINFDKAVVVDDHTVEIHTKAPVPAL
                     LYSLQEVKILPEHVYSKLTPTQAASEVIMGTGPFKFVEYVKDDYVKMERNDDYWGEKP
                     AFKYLVFRNIPEASTRVAELETGGIDVAQQVPMAQIESVNASGTAHTSAVSNGCRMYM
                     GFNHENPTFTKNVRLAMNYAIDWDAINQSFFYGAVPRMVTNVNRPWLNENLKPYPYDL
                     NKVNELLTADGYVKNAKGFWEKDGKELAPKIMVYYEQTSERYEVLLSIVDMFRKAGIN
                     AEPYYLDRAAAFEKLDKREVDDMFYIASCTSYEGQGDITDLKADSASNYGRWNNPEFE
                     ALFDQLLVEFDMNKRAELLNKMQEIVYNEAAIIPLYIMIDVWGINNKLEWEPNPTGRA
                     LMKSAKKYK"
     gene            complement(17579..17797)
                     /locus_tag="PK898_07515"
     CDS             complement(17579..17797)
                     /locus_tag="PK898_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19918.1"
                     /translation="MKRNPFFLFLAIVIILSMALTACQSSTETKPTPVFEFKQFNPTK
                     APVHPTVPSTVTSNPNNTVETEATKVPD"
BASE COUNT         5147 a         3978 c         3210 g         5536 t
ORIGIN      
        1 taatccgtca cgtaaatcag caagaccaag ctgccaagca cattcgtgca aatgttggga
       61 aaactcatat gccagaattc tctgtccggt gatgccgtca gcccgctgca tccccaaaaa
      121 aacgaaagca tccgttgata caatggaatc tgtccatttt gatttctcct ccgcagaaag
      181 aagctcaaac tccgattgag ttaaactcgt cggtttgatt tgaatatttt gactttttcc
      241 gcccggagtt attgtattta ctgcgatgtt atacggcata agttctaatg caattgattt
      301 ggaaaaacct tctatggcat gtttcgtggc gcaatatata ctttcctttt caaaaccttt
      361 cacaccggcc ccggaagaaa cattaatgat gcttccccct ccttgtttta tcatctgtgg
      421 tatcgccatt ttacacagaa caatctgtga acgaagattg atctttataa tttgatcgat
      481 ttcgtccaaa gaaagatcag taaaaggttt taacatgagg actgctgcat tattgatcaa
      541 gatatcgagc tgagaaaaat gatcgcttat atattgaaat atctctttga ttgcgttttc
      601 ttcgctgata tcaaaagcaa attcggtgac atttccacct tcttgacgaa taatttcagc
      661 ggtctgatca agctcggatt ttgttcgtgc aaccgcaata accgatgcac cttgacgggc
      721 atatgcgatc gcaattgatc ttccccatcc gcgaccggcg ccggtaatta atgcgacttt
      781 ttctttcaat aacatttacc gttctcctat aaataaatgt actttctaca gatcgttcat
      841 aacagcgatg gcagccggat cattattatt cttttctctt gttgataaat gcggttccat
      901 ttcgatgatc tgataatcca tttttcccaa accatgtttt tgagcagtag tcagtgtctt
      961 catttcgtca atcggtattt gtatacggtt tcgcgacgga tattgtttta atatttttat
     1021 tgcctcggta tcaatcgcca ccatatctcc cgatgccatc accacattgg gataaacata
     1081 atctccgcgc ccagcccaac ctacggttga tcttcttcca tccatgatga tgatgtccgg
     1141 ttgaaatgcc agatttattt ctgcgatcat ttgttccgtc ttgtctttat cacaatgcat
     1201 tatctcccgg tcttcaggag ataaccatcc cacgccaagt ttgatcgcac ccgaaaatcg
     1261 agcagaggaa tgacatcgca tattgggcag atacacccgg tgagtcgctt cataaatcgt
     1321 ccggggaaca tgaatcacct tccagaaatc accggaaatt tcaaccggaa tccaatcatc
     1381 tttttcaaaa tttataaaat caacaccgta tcttttcatc acaggcagaa cttttagttt
     1441 ttgaacaact tcttcggtcg gccatgcagc ccgaccgcac atttctccga ctttaatatg
     1501 caaaccgaaa tcagtcaaaa tctcaaatac tgccgctaaa aaatctaatc ctgtcgataa
     1561 cggtgtcgga tcaggacaat taaaattcgg cttcacgatg acagagtcgc cggaacggaa
     1621 ttttttgtta agtcccccca aggtttctat ggcggtatat accgtgtcaa ctaaataatc
     1681 gccattgaaa tgaggtacct ttacaaccaa aggtttatcg ttctgaatat acgcattttc
     1741 atttctcttg ttgcggggat aaacttccat attttttttc tttatctaat ttctctttaa
     1801 ttatcgaatg agattaaatt attgatcttt tatagaagat cagctgcatc tgcaaataga
     1861 tgcagctgat ttatcgaatt aaatgatcgc accatccggt ctatttgctt tttcccgcaa
     1921 tcataagatc gtttcgaatc tcatcaatcg ccccgaagaa acaacaccat tctccttcaa
     1981 tgacgctggg acgatagcga agtccgaata cttgcccatc ggccggagcg gtaagtgttt
     2041 ccagaacatc cccatataaa ttatagatat gtcccagtac agtgcctttc ttaatcatct
     2101 cacgaagcgg cacatcagct cctacaaaca ggccgcttgc atttgcaaga agagcaattt
     2161 gatgacctcg gtaccattta tcggcataaa cactttcacc ctcgatcata tgataatgtt
     2221 tcatgatatt aatgtatcca tttacatatt tcatgcagac ttcgacgaat tctgttgtca
     2281 gacatcggca atatccgcca tgctccactg tcagtgcacc ttttcccata ccggcaaaaa
     2341 ctgaggtcgg attccctgtc ccgccgacat tgcttgtgag tgccaaatcc cattcaggtc
     2401 ccagtgcttt tcccaattcc aagctctccg gattttcggg ggcaaagaaa gcgcggcaaa
     2461 gcattgaatg atcgccgccg gaatgaattg ccaaatgtaa atcagcatat ttggaagcaa
     2521 tttcatgaaa tgcccaagca actctttcgg aaggatatcc atccggtttc cccggataaa
     2581 atcgattcat atcatatgag aaagcatctc tcggatcgcc tcttcttccg tccattaatg
     2641 cttcaacatt caaagcaggt acaagtacca atgtgccgga cattttagcg ggatccaatt
     2701 caggcctatc cattaaaagc ttaattgaaa aaggtccttc cggctcatca ccgtgagtac
     2761 tgcctgtaat ccataaacat tttccttcat gtaagccacg gtagacaatg accggcaact
     2821 ctctttcaat tccatacata tttttaccga ttgctattgc gcctctcttc atttcgccag
     2881 gcatcactga aatattcccg atttttattg cttcgaccat actattttct cctcttttca
     2941 atttttaaat ttcttctttt tgcatggaaa tcagataaga cttcttatct tgttatgatc
     3001 tgattcctta tttcaaatta gcaaatcaaa ttctttaggg gttcattttt taaatatttt
     3061 tgaatgtttt ctaagactgt ataaatttga cgctcagccg cattcccttt tcctccggca
     3121 atatggggag ttaacgtcac attatctaaa gaaagtaacg gatgatcata aggaatgggc
     3181 tcatagacaa aaacgtccaa accggctcct gcgattcgat gattttttag cgtttcaaca
     3241 agtgcttctt catcgacgac acccccacgg caacaattaa taaaataagc tgtcggtttc
     3301 attaaagcga attctttcgc acccgcagct ttttctgtct ccggtgtcaa gggactgatc
     3361 atcaccaaga aatcagaccg tttgaacaat tcttctttac tgacatattt gacacccaat
     3421 tcttcttcca ttttctctgg taatggatgt ctcttaaagt aaagtatctc catttcaaag
     3481 gcatgggctc gtttagcaat ttcattaccg atatcgccaa agccaaaaat gcccaacgtt
     3541 gatccgttta attcctgcaa atcgggaatt ttcatccatt gaaaaccatg agatttttgt
     3601 gaggtaagtt tcggttcaat tcctaaatca cgataagcac cttttacggt cagttcatga
     3661 gccaagatca gtttctttgc acatgctaag atcagcatca ttgcatgttc agctaccgca
     3721 acggatcccg gtaatgagac aacggaaaca ttaattcgtc gtcgctttgc cgcctctaaa
     3781 tcgatattat cagcccgact ccccattttt tgaataagcc ttaatttcgg tgcggcattc
     3841 atcatacgtt catcaatagt taccttttga gtgactaaaa cttcggcatc cggcaatagg
     3901 cggaataatt cttcttcatc cggagttgag ggcataacca catccaattc atatcggatt
     3961 ccaaaatcat gccaattggc aatttgttct tttgtaccgc tatcggcaat aaccactttt
     4021 gtcatgtgtc ggtttatcct ctttctttta attacatgaa tttctttaaa ttacatttct
     4081 gtcatttcat tttttgtatc ttcggcttcg aataaagtcc tatttttgca tgtctcagac
     4141 ccaaagcagt aaaagcctcg gccattttaa ccgttgtcgc aacaggatca atgatcggga
     4201 cttttaactc tttttctaaa tcttctgaat aacccgccat cgatgaacag cccaagataa
     4261 tgacttccgc accgttttcg atcagttctc tggctgcttt tattccggct tctttaactt
     4321 tcagagggtt gttcgctatt tccacgacac ccatgtttaa agcctttaca ccggcgaaac
     4381 gactttctaa tccatgtttc ctaacttccc gtgacttaac agcttcttta tgcttttgtt
     4441 ccgtcaaaat cgcaaattta tttccaaaaa gaagggcagc agaaaaggaa cattcttcca
     4501 atccgaaaac aggtatatcc gaaatttctt ttaaagcctc aattccggga tcgcaaaaag
     4561 cggcattaat gacagcatca taaccggctt catttgcatt tttcacagat tggaggatat
     4621 aaggagtggc tatcgcttca tcatagaaag actcaagtga ctccggtcca tgcggaatac
     4681 cggtcacaac aatttcggta tccggataag ctgctttttt ggctgtttcc gcgattcttt
     4741 gagtaaccgc ttccgacgtg ttcggattca aaactaaaat tttcattttt attcggctcc
     4801 ttataaaacg atatctctta aagcaaacaa ccatttattt aaaattgaaa attatagaaa
     4861 acacttaaaa atcttcaact ttatagcgat atccgcgact tgtaatatat ggttcgatta
     4921 gttctacggt gaccccatga ttattttttg aatcaacaaa ggcttcaaat ccatcttctc
     4981 gatcgagata aattttattt gctgtcagag aaacacccgc ttttttgact gtttttacag
     5041 ctctccgaat atcatttact tcaaatgcca gatgttccag cccctctcca tgttgagcca
     5101 taaattgtgt tacacgattt tccggatcgg tactttcaac aagatgaagc cataatcggt
     5161 catttcccgg ttcaatcatg atatctttct gagttccgta atgttgatct gtccgcaggt
     5221 cggaggattt gattctaaaa agtttttcaa acttatttgc cgcctcaact acatttttca
     5281 ccgcaacacc gatatgttgt aatcccaata aattcggatt tgagactttt tccggctgcc
     5341 aataataact ggtaatatga ggctgaatca attcgaccgt aaaaccgatg gccgcatccg
     5401 gatagataaa agcttcgaat ccatccgctg cattaaaaat tttatcttca aataccggga
     5461 cacctattga tcgtaaatac gccacttctt cttcaatatc agtagtctca agagcgatat
     5521 gctccaatcc ttcaccatgt tgctgtaaga atttgtttac tcgagattcc ggattccaat
     5581 tttcaactaa gtgcagccat gttcgattcg gcatcatgat tcttgcatcc aattgaaatc
     5641 ctttcccttg attattcctg atatccgttg ctttaaatcc gaacaactta tcataagtag
     5701 atatagcctg atccagtttc tccaccgcaa tcccgatatg atgagctctt aaaatcattg
     5761 gttcctcctt aaatatattt ttgaaaaatc aatcttccgc gttattattt ttatctaaga
     5821 tattcgcttc tttttttcag gtatggacat tatttgtttc cgattggata ttcatatcaa
     5881 aaccgattct tttacatgac taataaataa aacctgattt tttccattca gccggatttt
     5941 catatccacg cgtcgtctga ggattgttta ccattgctaa tcttgtaaac aacttcgcta
     6001 acatatcctg accgacttcc aatgcttcaa gggcaacgcc gtttgaatag gaagtcagga
     6061 aatcaatcat tttttgctta tcttctttgt agaggttcaa agcgatttgt tcgatggaat
     6121 tttgaaattc atatctccta tgatcaaaat cttcccagac cggtttcact aaatcaaccg
     6181 tgccatcgta aaataaattc atgatgttcc cgatattttc aaaaagccaa tatgctgaat
     6241 caggtaaata aacgctgtta tctccggcta tgccaatctt atatgccgaa ggagtatctt
     6301 tatgacaggc ataaaatggg atatgcggac tactcgccgt ggctgctaat cctgcccaga
     6361 tcagcccacc gatttcattc ggcatccaac tgcgagattg ggttacccaa gaggtaatcg
     6421 tttgcggaca tccgatgatt cgagatttac tccaagccgg tgaatatcga taatgcccgg
     6481 ttacaggatc tacaatagtc ccgtagggca tatcggtttt cccatattca tccaaatcag
     6541 ttccttcata tgtatccttc aaaacatgaa tcaacatttc gggagttatg gatttggaag
     6601 gtttgacaaa aagcggatac tcttgagtag gttcgtattc atccaaattc aaatccgtgt
     6661 tcaataaact gatgcttctc caaattcgat gaagactata ataaggctgt tcgaattcgc
     6721 catgtttccc gcgcggacga ttcctgttat tggtcccata tgcacgagat acattaaaag
     6781 gctttccgga attcggatcc cataaaccgt gttgaatcgc aaaaggaatc aaatccggat
     6841 cacataaata attcgcggta tcttccggga aaaaatcagc gattctaaat gaattacctt
     6901 caacaacaaa actgtcatcg ggtacacgaa cagcagccca atgatgatct tgatattctt
     6961 catacaacca aacctcattc ggatccgcaa taatataatt gtcggtcctg gctccatatt
     7021 ctgctacatt ttgtccgagc cattcgatcg cttcgcgagc tgttttgcat cgttccaaaa
     7081 ttaattgcat acggtagtcg ccgatcacgg tttccggcat cggcgaaata tcttcaactt
     7141 cttttttcaa ccaaccgccc gtacttgcac cggcgcttaa ttgaaactcg ttgacgcctc
     7201 cggcaaccat tccgatatca tctccgtcaa catatcgggt tatagctata aatgcaaatg
     7261 tttcctctac ttgcggaatc tcaacattat ttgaatcggg gatgcgaatc aattcgtgtg
     7321 gttgatgttt ttttcgtgga tacgcaataa ttcgttgggc ttcggttgaa ttggaatcac
     7381 aattccgagc aaccattgtt gatccatcgg cactcgcttt ctttcccact gctatcatga
     7441 aacacataat ttttttcctc tctttgtatt agttcattct tattgtttat tgaattattt
     7501 gtccctttat gtgacaatat tttttcaaat gaatagaaat attcaaactc tttcgctcaa
     7561 attcttaagt accccggtca ttctttttgg ctgtttcata ggcctgaatg gatcaggctg
     7621 ccaaatgccc acatattctt gttgttaatt tatttctcaa ttcttaaatt attataattt
     7681 taagaaattt ttttgtcttt tgttcaaata ttgtaacata aatctctctt gtaagatctt
     7741 cgaaaattca tttttattcg ttatatgttt tttgataatt tatgaagaat ttttttatta
     7801 taaaacttct tatttcataa ttaagccttt ttctcgataa atataatata attttgaatc
     7861 gaagtaacac ttaaatatat tataagtaag gacgatgcaa gggatggata ttttcgagcg
     7921 tataaaaaaa gagcaagacg gatttagtaa gagttttaaa aaggtagctg attttatttg
     7981 ttcgaattac gaagaagttg cctttatgtc cattactgag gtcgctaaaa gaagcggcgt
     8041 cagcgaatcg gtaatcgtca gattttctgc ggcattggga tacagcggct ttatcgaaat
     8101 gaaaaagaat attgccgaaa aagtgaaaga aaatctgaaa cttccaaacc gtatgacatc
     8161 gtcatcctta tctgaaaata tgtctgccaa cgaaattatg acagctgttt tagcaaagga
     8221 tatggaaaat attctatata cctcaaaaga accttataac aaatctttcg aacaaattgt
     8281 gcgagaaatt catgatgcca aaaaagttta tattatcggg aatcgcgggc tatataaaat
     8341 tgccgaactc accgaactct tattgacacc gatcggcatc ccctgtatat ccatcaacgc
     8401 ggaagacagt atccagttcc aacagttaaa ttcgatgcgc aaaaattgcg ttttattagc
     8461 tttttcattg ccaagataca gtagtcacac atataccgca attaaaattg ctaaaaagta
     8521 tcattgtaag acaattgtta tcagtgacag cattttctct ccggacgcaa aagcagcgga
     8581 tctttcactt gaaattacag taaaatcata tacttacatc aattcgtatt gcgctattgt
     8641 ttcaatgatg aatgcaatca ttacagcgat aggtgttctt tataagacgg aaactctaaa
     8701 ttcgctgaac agattagaga attttctgca aaattttcag gtaattgaaa gttattcgaa
     8761 gaataattcg gaaagcgaga agcggatatc gcaatcgaaa accagttaga ttatttgagt
     8821 gaatgagata atcttttttt tcgaacaact tattttcgaa aattcttttc ctttttaatc
     8881 atattatttg cttttttaca atattttttc tataatatat atcagaggat aagggaagag
     8941 attaatttct tttagacccc tgtattccca aaaataattc acaatcgcca catcggaggt
     9001 aaaactatga gttgtaatgc acttgatagg cttcgttctg ataaaacacg aaccgtcggg
     9061 gaattaagta ttcgtacaaa actgcttgaa aaagcagcca ctttagatga tgtcattctc
     9121 atgggtcgcg gtgatccgga tcttccgacc ccaaagcata ttatcgaagc tgcgaaaaaa
     9181 gcaattgata acggagctac tcattatacg catgttcgag gaactattga acttcgcgaa
     9241 gcgattgctg agaaactaaa aagggataat aaccttgatt atgatcctga aaatgaaatt
     9301 acggtaaccg taggagctga agaagcggtt tttctgtccc ttttcggcat tttgaatccg
     9361 ggagatgagg tccttgtgcc cgagcctcgc tataattctt atgacgaagc gattttgatg
     9421 cttggaggag taccgaaagt tgtcaaatgt attccggaag agaactttcg cctgactcct
     9481 gaacgactgg aagcgaacat ctctccgaaa accaaagcca tttcgttagt caatcccggt
     9541 aatccggtcg gcatgtatga cccggaacag gttaaagaat tatgccaaat tgcaataaag
     9601 catgacctga ttgtcatcag cgatgaaatc tatgaaaata ttattttcga tgatacgccg
     9661 catctgagtg ttgcatccat tcccggtatg cgggaaagaa caatcacgat aaacggaccg
     9721 tcaaaatcat atgccatgac aggatggcgt tgtggttttt ttgcagcgcc cgctccgttt
     9781 tgtgaaattt taaccgagcc cgcccatacg atttcaattt gctgccccgc tccgacacag
     9841 gctgccgcgc ttgccgctta tacaggtccg caagactgtg taaaagaaat gagagatatt
     9901 tacaaagaaa gatgcaacat aatggcaacg gcacttactg aaatgggatt ttcgtttatt
     9961 cctccccgcg caggctttta tattttcact gatgtgacca gtctcggtat gactcctgaa
    10021 gaattctgta tgcagttgtt aatgcaggca aaagtactta tttttcccgg gtcactgttc
    10081 cttgatccta ccggtcgtta tgtaagaatt tctcttcttt ctccaacaga acgtattatc
    10141 gctgctgcgg aaagaatgaa aaatttcgtt aaacaatata aataacttcg gtgaaatcat
    10201 gaaattgaca actgattggt acattaaaaa aatgtcccaa atccaatcta aattatcgga
    10261 agaaaaattg gatgctattc ttcttcttga tccatataat attttttacg cttcaggatt
    10321 cttccatcaa tctaccgaaa ggcctttagg tctgctgatc cctcaaatgg gggaaccgtc
    10381 tttatatgtt cccaaactgg aacaggaaat ggcatcggaa acctggatta aatccattca
    10441 tgtttatttt gatttccccg gtttaatcca tccgattgcc tggatgctta aggagaattc
    10501 acaatatagc catataggcg ttgaccaact aaattatcgc caatggaaaa tcgcacagga
    10561 aggttgtccg aatattcaaa tcagcgattt ggtatataca atgcgactgg taaaagatgc
    10621 cgaggaaatt caaattctgg aatgcgcttc tgtttatgcc gattatatgg tcgaaatgac
    10681 aaaagatgca attattgaag ggttatccga attagatgct tataattatg cacgcgatca
    10741 taccgtaaat aaaatgatca aggaattggg tgagttgaca tttgcgaatc aaggaaccac
    10801 caacggcgcg gttctctacg gagctcattc tgctcatccg catggtctga tgtcatcaac
    10861 attgaaaccg aaaccgggag atgtaataga agctggtttc ggtgcattgg tttgtaccta
    10921 cgaatcggaa agcgaacata ccttcattta tggagaaccg gatcatcaga cggtttctta
    10981 ttttaatgcc atgtacaatg cttggaaagc cggattggag tctgcaaaac cgggtgtgaa
    11041 atgctgtgat gtaaacaaag cggcattaga agaaattaga agagcgggat atgaagaatt
    11101 tcttcgtcac cgaatgggtc acggaaaagg attacaggaa cacgaaccgc catgggttgc
    11161 cgaaggtgac atgaccgttc ttaaacccgg aatgatcatc agtgacgaac ccgggctata
    11221 tgtccccggc tttggcggat ttcggcattc cgatacattg ctgattaccg aagacggctg
    11281 tcgccgcctg acgaaatatc ctcgcgaatt agaagcatgc attataccga ttaaatagga
    11341 tatatcctct ctcctttttg ttataaacta aaccggagat gggcaattgc tcatctccgg
    11401 tttagtttat aatcttcaac aatttttgat cgtttttttg tgctttcaac ttttaagcga
    11461 aaaaaatctt attgagtcta tcagcaagca aaatttccct cattgtaatt ccccgggaat
    11521 cgattccctg tatttcaacg gtcaacctgc ttccttcgat aatcaaatta cattgttttc
    11581 tattcatttt caaatagttc caaatttttc gaaccatcat attagcgata tcgttgttcc
    11641 cgctttcagt tgatcgtatc aattttaaat tctcccgccg ccatcctttc agagtgtata
    11701 tccattggtt taactgatcg tcagttaata tttcgtcgat taaactggtg tattttatct
    11761 gatgatcaaa ccattcggta attcttctta atcgctcgat tcgatgccaa ggttctccgt
    11821 ttcttgaaag attatgccct tcgcgtggaa atcgaaccat attaacatca cggtttccgt
    11881 ttctccttaa atgagtaaaa aagctctctg cctggctgat tgggcatcga tagtctaatt
    11941 cgctatgtaa tatcaacaac ggtgtggtca cattttttgc atgcgcgatc ggagaatatt
    12001 cccaaagttc ttttaaattt tcctcccgcg gaaccccgcc gtagtaccat tctaccgatt
    12061 cggggatatc accgctgcca aacatattta attcatcata gactcctctt tgtgccacag
    12121 cggcagcaaa cctgccggta tgtgtgacga tccaggcggt caaaaatccg ccgtatgacc
    12181 ctcccgttac acataatctg ttttgatcaa ccgacctttc tttggcaaga aatgcatcca
    12241 aaccgttcat aatgtcagcc atgtcggtat atccgccctt acctcttaat ttttgaaatc
    12301 gggcaccata tccggctgaa cctctcggat tacagaagaa gacaaaatat cctcttgaac
    12361 acaatacttg aaattcatgc caaaaagagt ttccccacat gatttgcggt cctccatgaa
    12421 tttcaacaac cgcagggtag gttttttgcg gattaaaatc ttttggttta aaataccatc
    12481 cttgaatact cagaccgtct gtaccggtaa actccagttc atcaggaatt gaaaaggcat
    12541 gagaagtttc aaaatcttta ttgaaatgtg tcagttgatt ttcttctccc gtatcgacat
    12601 tttttataaa taaatcaccg ggcgtaatcg gcgacgatac ggaaaatgca atcattttac
    12661 cgtccgtact gacatcaaat tgctctacca tcctctttcc gtaaacaatt tctctcggcg
    12721 tcccccctgc taaatcgttt tgaacaattt ttccgtcacc atgatcgtga actaaaaaat
    12781 atatatgttt gctgtcacta tcccacttaa aattgataac ggtttgatct tccgtactga
    12841 taatcttatt ttcagctttt tccacacatg agtttacggc aacatagtaa ggttcttcat
    12901 acggactttc tgctttttgg acatagaaat aagcaagcca ccttccgtca ggtgaaattt
    12961 ctatctctct tccctcctcc gagacttcag ataaaataac ctcttctttt gctgagtaaa
    13021 tatcaaattt tagaactgaa gaccataatt caaaattttc ctcaccggat gactgatctc
    13081 tttttgaatt agaaaataca aattttgagt cctttgacca ttcgggagcg ccatgatgat
    13141 attcaccatt cgataaacgc atcagagaac cgccttttga ggaaataaca tagatatgtt
    13201 ggtagcatcc gtcaaaaaaa gcagtaccgg ttcgataagg aagctgggtg accacacgcg
    13261 gatcttgaag ttcctgccgg tgcattttcg accattcttt cggtacttct ttcagccctt
    13321 tattatataa aactcctaaa tcttctaaat gatgctcatt caaagtggat agagaattaa
    13381 aagcgatcca tttagaatcc ggtgaccaat ttatattcga aactcctccg accatatcgg
    13441 ttatttgaac agcttctccg ccatttaatg aaatgacata taactgtgaa gatccgtccc
    13501 gtgatgaaat gaatgccaag cgtgtgccgt cgggagacca gcgaggattt tgatccgaag
    13561 tgccgttcgt aaacggatac ggttccgatt ttccatctgt atcaaccatc catatagtgc
    13621 ggtgatagtc attcccttct tcgtccgctt gcattcgaac aaatgcgatt tttttcccgt
    13681 ccgtactgaa ttgtggatct tcgatgatcg aataatcgaa taaactctct gggcaaattg
    13741 gttttaacat tttgctgtta tctttatttt ccattctatg actgcctatt cttataaaaa
    13801 tcttttttat aataacgttc ttggatcaaa aacatctcgc aaccaatctc ctaatagatt
    13861 aattcccagg acagtaattc cgattgctaa accgggtaaa gtggtaagcc accatgcttg
    13921 ccgaatatat tcacgaccct ccgaaagcat catcccccat gtcggtgtgg tcggcttaac
    13981 tcctaacccc aaaaaactca aagttgcttc ggtaataatt gtcgaagcga cagcaaatga
    14041 tgcaaccacg ataacggaat taactacgtt tggtaaaatg tgcttaaaaa taatccgcat
    14101 attacttgca ccgagcgagc gtgccgattc tacaaattct ttctcacgta atgaaagcac
    14161 ttctcctctg acaacgcgag catacgagac ccatccggtc aagcccaaga ccaaaatcat
    14221 gtttcccaat ccgggaccga tcacagcaag gacagccaac attagtaaaa taaagggaaa
    14281 agccaataga atatcagcta tgcgcataat gatatcatcg actatcccgc caaaatatcc
    14341 ggcgattaat ccgagtgtta ccccgatgca tccggaaata atgaccgacg ttattccgac
    14401 gagcaaggat actcgcgctc cgtaaacgat ccgagcccaa atatctcttc ccaatgcgtc
    14461 tgttccaagc ggatgactca caaccgaata acggaacggt acggggggtt tcagccgtgc
    14521 cattaaatca gtggttaccg ggtcatatgg cgttaatatc ggagcgagaa tagcaaataa
    14581 cgaaacgata aaaattacga tgatcgcaaa ttttgctttc gtcgattttt tcaaatcata
    14641 caaaaaacgg ctgttactcg atttttttgg ctttttaatt tctaccgcat gcttttcaga
    14701 ttttgttgta ttgttagatg gttccatcat attatttcct acttatcctt tggttattga
    14761 agtctcacgc gggggtcaag tattgtgtag gtaaaatcaa ccagtaaatt cgcaaaaacg
    14821 aacatcaatg caaaaagtgt cacacaagct tggaccatca taatatcttt cccatttatg
    14881 gcattgacgg tcaacattcc catcccgggc catgcaaata ttgtttcgac aaccaaagct
    14941 ccgctgagca ggcttcccaa atccatgccc accattgtca caaccggaat caacacattt
    15001 cttaaggcat gttttatata aacatttctt tccgtttgcc cttttgcacg cgcagtccga
    15061 atataatcct ctcccaaaac ttccagcata gaacttctga tcaatcttgt atttctcgca
    15121 atcccataaa atgacagcgt aattaccggg aggaccatac tccgaattcc ctcatatcgc
    15181 cccgaaaccg gaagccattt tagattcacg gaaaatatca tcatgagcat taacccgatc
    15241 caaaaagacg gcatcgactg gccaagtaag gccagaacca tagaaaagct atcccatttt
    15301 gtcccccgct ttagggcagc aaatacccca agcgggaaag acaccaataa ttgcaaaatc
    15361 aatgaaacca cagtgagttg cagcgtagcg ggaaatcgct cgacaatgat tttccagttg
    15421 tcctctttat atttataaga ccaaccgaaa tcgcctcgga cagcattttt tagccaatca
    15481 aaatattgaa ccaaaatcgg acgatcaaac cccatgcggt gtcgaaatgc ttggatatct
    15541 tcttccgtca tcccgcgcat atcaccgatt aatacataag tcggatcacc cgctaaaaaa
    15601 ttgattgaaa atgccgcaat tgttataccg aaaataacta taatcgcttg aagcaaacgc
    15661 cgagctgtat atcgagccac tattacctcc taacaagctg cagaaagtta tcgctggatt
    15721 tagattttca aatttttacc actattcact tatctcgaaa aatgatcctt cgaagtaagg
    15781 cagtgaaata tgctcactgc cttactcgaa tgtttatagc gtaaataata ttattttgtt
    15841 gatccggaaa aatttactta tattttttcg cagatttcat cagcgcacga ccggtcggat
    15901 tgggttccca ttcaagcttg ttattgattc cccaaacatc gatcatgata tacaaaggaa
    15961 tgattgctgc ttcattatat acaatttcct gcatcttatt aagcagttca gcccttttat
    16021 tcatatcgaa ttccactaac aactggtcaa aaagcgcttc aaattcgggg ttattccatc
    16081 gcccgtaatt gcttgcggaa tccgctttca agtcggtgat atcaccttga ccttcatagc
    16141 tggtacaact ggcaatataa aacatatcat cgacttcgcg tttatccaac ttttcaaacg
    16201 cagcagcccg atccagataa taaggctcgg catttattcc cgctttgcgg aacatatcga
    16261 caattgacag taaaacctca taacgctcac tggtttgttc ataataaacc ataatcttcg
    16321 gtgctaattc tttgccgtct ttttcccaaa agccctttgc attcttaaca tacccatcag
    16381 cggtaagaag ttcattgacc ttgttcagat catatggata tggtttcaga ttctcattga
    16441 gccacgggcg gttgacattt gtcaccatac gtggaacagc tccgtagaag aaggattggt
    16501 tgatggcatc ccagtcaatt gcatagttca tcgctaagcg aacgttcttc gtaaaagtcg
    16561 gattttcatg attgaagccc atgtacattc ggcaaccgtt tgataccgca ctggtatggg
    16621 cagtccctga agcattcacc gattcgattt gagccatagg cacttgttga gccacatcga
    16681 tcccgcctgt ctccaattca gctacccgag ttgatgcttc cggaatattt cggaacacca
    16741 gatatttgaa tgcgggtttt tccccccaat agtcatcgtt ccgttccatt tttacataat
    16801 catcttttac atactcgaca aatttaaacg gaccggtacc catgatcact tccgatgcag
    16861 cctgagtcgg tgtcaatttt gaatatacat gttcgggtaa aatttttact tcttgcagag
    16921 agtataataa agccggcacc ggtgccttgg tgtgaatttc gacggtatga tcatcaacga
    16981 caacagcctt gtcaaagttg atttgattat tgagatagtt ggtattgccg atgttgtaat
    17041 catccatcag catgcgctcc atcgtatact tgacatcctt tgctgtgaac ggttctccat
    17101 cccagaactt gacatcatca cgcagcttaa atgtccagac ttttccatct tcacttgtag
    17161 accattctgt tgccaggcag ggtacaatgg taactgtctg aggatccaga tcaatcaatg
    17221 gttcaaacat gtgctggaaa ataccggagg cattcaaagc cgaatgaatc atatagtcca
    17281 gattgtcaat aattgtcgtc aacgcgaccg ttacggtagc ttctgtgtct acttcttgtt
    17341 gagccattac cgttaccggc aatgccagca gacagagcgc caataaaaca aacattattt
    17401 ttttcattgc atcctccata aattatggtt aattcccgac aatgacatat caaagtccgg
    17461 gttatgcgat ttgttttggt ctagaaactt ttattctatt tttttacatt aaactaattt
    17521 ataaaaattt tcttgtgatc aattaataaa aaaaatcttc atcactcctt cctggtattt
    17581 aatcgggtac ctttgtcgct tctgtttcaa ccgtattatt tggattgctt gtaacggtag
    17641 agggaacagt cggatgaaca ggtgccttcg tcggattgaa ttgcttgaat tcaaagacag
    17701 gggttggttt cgtttctgtc gatgattgac atgccgtcaa agccatgctt aatataatga
    17761 ctatggcaag gaaaaggaaa aatggatttc tcttcataat atgtgctcct aaactattgt
    17821 ttcaacaaac gacaaaaagg aaatttattt ttcttattat accgacattt t
//
LOCUS       DAOSMM010000048        17545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3256_length_17545_cov_15.590337, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000048 DAOSMM010000000
VERSION     DAOSMM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 17545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17545
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_17545_cov_15.590337"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..2434
                     /locus_tag="PK898_07520"
     CDS             368..2434
                     /locus_tag="PK898_07520"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOU19919.1"
                     /translation="MSNTTGWLIFSLICGIASLILGLYLFFWVKKQDAGDEKAQEIAG
                     WIHGGARAYLKKLYTALIIVATIMAVILAVVYTLDGSGRGIQTAVCYLIGALCSSMAG
                     YLGMEVAVRANVRSATAASRDLSTAFNVAFRGGAVMGFAVVGIATIGMSAIYLITNQS
                     QLVLAFSLGASSLALLAKAGGGIYTKTADIAADLVGKVEVGIPEDDPRNPAVVADNVG
                     DNVGDVAGMGADIFDSYVASAVAAMLLGAAMGEPHYTILPLLLCALGIVASIIGVAMV
                     RVGNDGKPGKSLNMGTFITCGAFAALMAIAILLVPQIDNNFIWAILVGTAVGVIIGQT
                     TEFFTSDEYSPVKLTAEYSGSGAAITIISGMSYGLISIVPSIIGIVIAMLISFFVCGG
                     MGNFPMGIYGVGISAVGMLAVSGMIVSADAYGPIVDNARGIAEMSGMDEEVIDRSDIL
                     DSAGNTAKAITKGFAIAAAALTVLALFAAFVSEVNLALAPVGKAFNFEDASLLNPLIL
                     AGIFLGAMCPPLFSALTMNSVTKNAFAMIEEIRRQFREEPGIMKGTVRPDYNRCISMA
                     ASGALTSLIVPAIIAIIFPLIVGFVMGPKGLTGFLGGSIITGVIFALFMSNAGGLWDN
                     SKKYIESGQFGGKGSEAHKAAVIGDTVGDPFKDTAGPSLNTLITVMSLVASTFAPVIA
                     LYHLIP"
     gene            2580..4388
                     /locus_tag="PK898_07525"
     CDS             2580..4388
                     /locus_tag="PK898_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19920.1"
                     /translation="MTTPIIDFDLTKIKTKNRIAGIWQMMSGYRLKYFLAIFSQLFST
                     VFKSSVYILLSWFIDTWLISNERPIPAYGIGLGFVLLALCEGLFTFYSNSTANYIAEK
                     MALRIRNNLYDQIQRLNFAYHATVDTGDLIQRATSDVDAVRRFFSEQAIGIGRICFLY
                     AVNFIAIYRMDSSLALSTLMIVPLVFLMALFFFRKIGKRYEAYQNQDALLSTMLQENL
                     TGVRVVKAFARQDYEIEKYRKENWKKFLLGKKLVFTEALFWPSTDLLCGFQMVISYML
                     GAYATISGRISVGDFVAFQALIVWIIWPIRNMGRLIVQMSRAFISFQRIMEIFANEQE
                     PLDEGTVMPRKVPEGEINFDHVSFAYQPENETLHDLSFHVSPGQSVAIIGPTGSGKTS
                     LINLLPRFYEYTSGRITLDGMELNRIPRNYLRKHIGIVEQEPFLFSRSIAENITYGAG
                     REVSTDEIIQAAKAAAIHESILSFEKGYDTLVGERGVTLSGGQKQRIAIARTLLKNPK
                     ILLLDDATSSVDMDTEVEIRAALNELMKDRTTFIIAHRIQTIMQADLILVMDQGKILQ
                     SGTHQELVQQEGFYRQIFNLQSQIEEALEKELSHGG"
     gene            4378..6192
                     /locus_tag="PK898_07530"
     CDS             4378..6192
                     /locus_tag="PK898_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19921.1"
                     /translation="MADNQFEEEDFQSKFNGNVIKRIFEFITPHKRWFFLFLFCTLIT
                     SVMDSLQVYLNKLLLDNAIVPGLKSQVLPYLKYYGLTVIVQAIGVFTFVYLAGMLSER
                     IWYDLRQKMFDHLQKLSLSYYAVTPVGWIMSRVNSDSFKIADLVTWGMVDSSFAIVNI
                     LISGGFMLAINWRMALIVFAVFPIMLYVSWQFRKRILKEFRLVRKLNSKVTGAFNENI
                     MGVRVVKAFQREDQNLKEFNDLTYPMFQSAYKAARLNALFLPCIQLISSVALGLIIWS
                     ADWQIRSGLMTIGGIQAFVNYVTSIMWPVQDLARVYGEMQQSVASGERIFSLLDTQPA
                     IVDKPDAFEPQTLCGEIEFDHVSFWYDDGDGRPVLEDFSLKVNQGETIALVGSTGGGK
                     STIVNLLARFYEPKKGVIRICGRDYRDYKMESIQSRIGMVLQTPHLFSGTIRENIRYG
                     KLDATDEEIEQAARQAGADTFIRSFPNQYEEQVGEGGNLLSFGQKQLISLARAILADP
                     EIFIMDEATSSVDTLTENLIQQGMDHMMKGRTSFIIAHRLSTIKRADRILVIEDGKII
                     EEGTHFQLLAKKGKYYHLYTQQYREEKERDLKLNPLSA"
     gene            6647..9640
                     /gene="ygfK"
                     /locus_tag="PK898_07535"
     CDS             6647..9640
                     /gene="ygfK"
                     /locus_tag="PK898_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOU19922.1"
                     /translation="MSDRMTCIPFSQLLNWVFDEYQSQGTVFGVHQPYKAKEGKTLEL
                     FGRKLETPIGPAAGPHTQLAQNIISAYFAGSRFFEVKTVQIIDGEDLPVSKPCIDATD
                     EGYNIEWSTELTVPDAMGEYIKAWVILHLMAKEMGLGAQDGFQFNMSVGYDLAGIRSE
                     KINTFIDSMIEAKDTEVFKNAIRTAKANLSKFKHVTEADIDAIPSAICNSATISTLHG
                     CPPKEIESIATYLITEKHLNTFVKCNPTLLGYDFARKTLDEMGYDYVSFGRFHFEDDL
                     QYQDAVPMFERLIDLAASHHLQFGVKLTNTFPVDITRHELAGKEMYMSGKPLFALTSA
                     LAAKLSRDFDGKLRISYSGGADAFNIEKIVGAGIWPVTMATTILKPGGYQRFRQLAEK
                     LEKQGYHSFEGVNVAAISKIAEDAKKDKHYLKPAKMPPSRKNNRKVPLLDCFIAGCEE
                     GCPIHQDIPTYIRLTGEGKYKEALQVILDKNPLPFITGTLCAHPCMTNCVRNHYESPV
                     NIRGVKLEAAKQGYESVMRELKPTGHSEKNVAIVGGGPAGIAAAFYLLRAGAKVTIFE
                     KRPRLGGIVRYVIPRFRIDPATVINKDLDFIRQLGVEIHLNTEVSSVEDLKTGLFDAA
                     IIAIGAHVPAQITIEGCTPLHALSFLEEFNNTKGKMNIGKNVVVIGGGNTAMDVARSA
                     KRTEGVENVYLVYRRTKRYMPADEEELQLALNDGVIFKELLAPEKWENGELICRVMEL
                     GEYDESGRRSVVETDQYVRVAADTVISATGENVSTDFYQANGIAVDAKGKPVVNPKTL
                     ESSKENIYIVGDGLYGPSTIVQAIANSMKAASAIVGFDVTSDVFQPTPKESIFPKRGV
                     LIKEEDATHESERCLSCSTVCENCVEVCPNRANVSIHVPGRSEAQIIHVDYMCNECGN
                     CETFCPYNSGPYKDKFTLFANEKDMENSKNAGFVVLDSKAKKFKVRLFGEESVITPES
                     RDFPEGIMDLMNSVCDNYPYLIFEK"
     gene            9833..10618
                     /locus_tag="PK898_07540"
     CDS             9833..10618
                     /locus_tag="PK898_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU19923.1"
                     /translation="MAEKESYLHRVFALLDCFTAERPELGVREASRMLGISRSATGRL
                     MSELRNEGVLTQDLDTSLYSLGGRVIRWAGVYEAASDLSVKALPYIRQLLNKTNETVS
                     LYVVEGNERVCIERLESRQNVRIVEPIGQRLKLYAGSGGRAILAFMPPEEINRILKLA
                     EQEITEGDVQEQIREIREHLEEVRRTGVAISHGQWVDGASGIAAPIFDESGLPIGSVS
                     VSGPTNRFRDQKTLDAYRELLLSTMEKLSNEMGYRSDRIIANP"
     gene            10663..11988
                     /locus_tag="PK898_07545"
     CDS             10663..11988
                     /locus_tag="PK898_07545"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HOU19924.1"
                     /translation="MILEDRIQADVSTWRERVNRLTKEHGDFVISNVTVSQIFSGIRG
                     VPIQVSDISYVDPEKGIRYRGYTINEILSLLPKLPDTNYPLVGGVYHLLMGNELPTME
                     KALAIENEWKNRSEVPDYVFRIIDSFPENASTMAMYSAAILSMSSDSKFSKGYHDSLR
                     KEDYWKAMLDDSLDLTARSPQIAAYIYNKKYRNNEQIGPDKSLDYAANFAHMIGKGDD
                     PLYADLMRLFILLHADHENANVSAHTAHLVGSALSDIYLSCSAGLNGLAGPLHGLANQ
                     ECFNWLLALYNTYQGVPTKQQVTEYIQKTLDEGKVIPGYGHAVLRCIDPRFEAERQFA
                     EKYFPDDEYFRTAMVVFETAPDMLKATGKVKNPWPNVDALNGTLQYHFGVTQSDYYTV
                     LFGLSRMLGFTCHIVWARALGKPIERPKALTTRLLESMIAEEKKQEAAK"
     gene            complement(12065..>12649)
                     /locus_tag="PK898_07550"
     CDS             complement(12065..>12649)
                     /locus_tag="PK898_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU19925.1"
                     /translation="HVVENGQSLWSIATAYRVTIEDIATWNNIVDIESISLGQKLLIP
                     ERGSAGQAPTSTVLPTVLPTADSEGKYRHVITEGETLSSIAKLWGTTIQELMRWNGID
                     ESTTLGVGWKLFIPVTPTVTVPPTLTPLPSETPSPTQTLTMTPLPSMTPTPTLIAANH
                     LPKASTKTYFIGIASIILLTTVFFFSGRYFYRRK"
     assembly_gap    12650..12749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13055..14251)
                     /locus_tag="PK898_07555"
     CDS             complement(13055..14251)
                     /locus_tag="PK898_07555"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19926.1"
                     /translation="MKKFAFLSGLIFLSLVSGCTYHSPEPINLDATIQAGISSALEEK
                     DLLDQAKLDQSQQTQNTQIAAEVDRKVSSLATEVYLDMQENVLPEIGGTPTLSLGSGS
                     GSAACTNKFEFLSDVTFPDGTMTLPLTTFTKSWYVQNTGTCEWTKYYSIVYLSGDNVG
                     TAKEFPLFTSDKLLKNGESAIISVIITAPEKQGEYSTYWALKSPDGQIFKGGDSAEGY
                     PLSSKFKVGSQYNFYENLAGAICSDDSGLFFCGSSDRSSGRGVAYYSASPTVESNYAG
                     QPSIVIGPPLKENGKTRVTFGPIRVPRGTWLRAAVSCPPNAPGCDNFVRLFVQVEGST
                     EQRVTETQENNDGYVTEWNIQLSDYGYHDQSLTYTFEVQANGGSDADDLIIIQMPRLT
                     DVAPVK"
     gene            complement(14248..14955)
                     /locus_tag="PK898_07560"
     CDS             complement(14248..14955)
                     /locus_tag="PK898_07560"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU19927.1"
                     /translation="MQVNPLMLKIIAFILFAFLICGCDSIVLSTPSPTPDFEKTVYWL
                     VDEAVSKASTQIMFDVDQKLATMEAKQPSLIENITETPTPTASVVAAPFRGTSVPEAL
                     RKKENSVVENTPASCIDSLKFVEDITIPDKTVVKAGKPFTKTWKIQNTGTCTWTEAYS
                     VVFSDGDKMSALDRTGLPVGTSVKPNDTIEVSVYMIAPEQSGSYAGYWLFESPSGIRF
                     GAGSNRDKPIWVKVEVK"
     gene            complement(14962..15315)
                     /gene="folB"
                     /locus_tag="PK898_07565"
     CDS             complement(14962..15315)
                     /gene="folB"
                     /locus_tag="PK898_07565"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOU19928.1"
                     /translation="MDRIILKDLLARGRIGISDKERERPQDILINIEMQTDIHQAGLS
                     DNLLDCVDYSETAKAVLALVEDNNRKTLEALTTDIADYCLQHPLVRSVKVRVEKTAAV
                     RFAKSAGVEIEREKK"
     gene            complement(15317..15847)
                     /locus_tag="PK898_07570"
     CDS             complement(15317..15847)
                     /locus_tag="PK898_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19929.1"
                     /translation="MPKKKILLFILIGVIVFSFFVIACQTRISDSGYSLKTVGKKIDS
                     NIYILDVTMLGPVTSIKDLSAEGTAKQYSDIYGYGNMRLWQDGKGLVSVHVNSINPKL
                     DYVQPGTDIVLKTTDLKILAIAEGYRFEVKCRNDYEPVASLEDNEMTSERYDTYELDY
                     CRMTTPEITLEKNIEE"
     gene            16073..16888
                     /locus_tag="PK898_07575"
     CDS             16073..16888
                     /locus_tag="PK898_07575"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19930.1"
                     /translation="MNEENQIGNDYSILTVIILLVYVLSTFITSYIVTIPIHIGNFSL
                     ILNWKMIDLITPGAGLLAGIGVFGLVDERSSKSKAEKMLHSVIPFATTVSLGIILRNV
                     AMSAGWVMILILGGVLLYLVFMSECIICNPADPRISVVSILLTGLAYATFVITAIGVR
                     SNISRLILEFPLILLSSFILAMRILSLYESVKDIEIKAAIISLITAEAAAALHYWPIQ
                     PLSYSLLLFLGFYILTNIVIILPQKGSLRNAFKSQLWILAVSIILMVFIEIQK"
     gene            16905..>17545
                     /locus_tag="PK898_07580"
     CDS             16905..>17545
                     /locus_tag="PK898_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU19931.1"
                     /translation="MNWEILISLIMSYLIGSIPFGWIIVWIKKHQDIRYHASGKMGTS
                     NVIRMTDVPTGIITFFLDLFKGYAGVWVVRLIMDPAPAWMEAVGGFLAVLGHVYSIFI
                     VEKRRDGRYYFRGGAGGMTSMGAAIALWPPVILYSGIPSLLIYFIFGYASVATMSINV
                     VALICFIVRAALGLDPTWWYAVYAFFTLLLVLKALRPNIRRLRRGEERVMGFS"
BASE COUNT         4760 a         3515 c         4135 g         5035 t
ORIGIN      
        1 acagacaacg gatccaatct tcttccgacg gactgcgtaa aatctgaatt tccatgaaga
       61 atctccttaa tattaaggtt ggctaattat aacccttcaa aatccttccc gattcgattt
      121 cattgaatcg attattctga agatgaagga tgaaaacgga taacaaaatc cgccgaaatg
      181 tctgacaaaa gacatctacc aaaatccgac gaatgtgcta aaatataatc gcttaaaatg
      241 ttctgaaaaa tagccttctt ttttatttcc cggggaaaaa agaatatttt tcgaacattt
      301 ttactgtagc atagcggaaa ccccttttcc gcatccgtat ataacatcat tttttaggag
      361 cgtttgaatg agtaatacaa cagggtggtt aatctttagt cttatttgtg gtattgcgtc
      421 actcattctc ggcttatacc tattcttctg ggtgaagaag caggatgccg gagatgaaaa
      481 agcgcaggaa attgccggct ggattcatgg cggagcaaga gcttatctca aaaaacttta
      541 tacagctttg attatcgttg ccacgatcat ggctgtgatt ctggctgtcg tctacacttt
      601 agatggcagc ggcagaggga ttcaaaccgc tgtctgctat cttatcggtg ctctttgctc
      661 ttcaatggcc ggatatctcg gtatggaagt cgctgttcga gcaaatgtac gctccgcgac
      721 ggcagcatcc cgagatttat ctaccgcttt caatgttgca ttccgcggag gcgcagtaat
      781 gggatttgcc gtggtcggaa tcgcaacaat cggtatgagt gccatctatt tgattacgaa
      841 tcaatcacaa ttggttctgg cattctcttt aggcgcttct tctttggcgt tgctggcaaa
      901 agccggcggt ggtatttata caaagactgc ggatattgca gccgacctgg tcggtaaggt
      961 ggaagtcggt atcccggaag atgatccgcg aaacccggct gtcgtagctg ataatgttgg
     1021 tgacaatgtc ggtgatgtcg ccggaatggg tgcggatatt tttgacagtt atgttgcctc
     1081 cgctgtcgcc gcaatgttac tgggcgcagc gatgggagaa cctcattaca cgattttgcc
     1141 attgttactt tgtgccctcg gaattgttgc ttctattatc ggtgtggcaa tggtcagagt
     1201 cggaaatgac ggaaaacccg gaaaatctct taatatgggg actttcatca cctgtggtgc
     1261 ttttgcggca ttgatggcta ttgcgatttt acttgtcccg caaatcgata acaacttcat
     1321 ttgggcgatt ttggtcggta ccgcagtcgg tgtcatcatc ggacagacaa ccgaattctt
     1381 tacctccgat gaatacagcc ccgtaaaact gaccgctgaa tattcgggct caggcgctgc
     1441 gattaccatc atcagcggta tgagctacgg acttatttct attgttccat ccatcatcgg
     1501 aattgtgata gcaatgttga tttccttctt cgtttgcggt ggtatgggca acttcccgat
     1561 gggtatttat ggtgtcggta tttcggcggt gggtatgctt gccgtgtccg gtatgattgt
     1621 ttccgcagat gcttacggcc cgattgtcga taatgctcgc ggtattgctg aaatgtccgg
     1681 aatggatgaa gaagtgattg atcgttcgga cattcttgat tccgcaggta atacggcaaa
     1741 agcgattacc aagggattcg cgattgccgc ggctgcattg accgtattgg cgctgttcgc
     1801 cgcatttgtc agtgaggtga atcttgcgct tgcaccggtt gggaaagcct tcaacttcga
     1861 agatgcttct ttgttgaatc cgttgattct ggccggaatt ttcttgggtg cgatgtgccc
     1921 gcccctcttc agcgcactga caatgaactc tgtgacaaag aatgcattcg ccatgattga
     1981 ggaaatcaga cgacaattcc gcgaagagcc gggcatcatg aaaggcactg tgagacccga
     2041 ttataaccgc tgcatcagta tggctgcgag cggtgcgttg acctccttga ttgttcccgc
     2101 tatcattgcg attattttcc cgttgattgt cggatttgtt atggggccga aagggctgac
     2161 cggatttttg ggcggctcca ttattacggg tgtgatcttc gcgttgttta tgtcgaacgc
     2221 aggcggtttg tgggataatt ccaaaaagta tattgaatcc ggtcaattcg gcggtaaagg
     2281 atcggaagct cataaagcgg ctgttatcgg tgatacggtc ggcgatccgt tcaaagatac
     2341 agccggtcct tcattgaata ctctgatcac tgtaatgtcc ttggtagctt ccacttttgc
     2401 tccggtgatt gcactttatc atctgattcc gtaaattact ttgttcttga tgtgattaaa
     2461 aagcctgctt gattcaagca ggcttttttg atgggaatga gtcatttttt cgaatgttcc
     2521 tgtataatac cctttgctgc gcggtaattc gtgtaaatta tgaaacaagg taacttattt
     2581 tgacaacgcc gataattgat tttgacttga cgaaaataaa aacaaagaat cgtatcgcag
     2641 gcatctggca aatgatgtcc ggttatcgct taaagtattt tttggctatc ttttcgcaat
     2701 tgttttccac ggtttttaaa tcaagcgtct acatcttgct ttcctggttt atcgatacat
     2761 ggctcatctc gaatgaacga ccgattcctg cttacgggat tggtttgggt tttgttttat
     2821 tagctctctg cgagggtttg tttacatttt attcgaacag cacggcaaac tacattgccg
     2881 aaaaaatggc attgcggatt cgaaataatc tttatgatca aattcagcgg ctgaattttg
     2941 cctatcatgc cactgtcgat acaggtgatc tgattcaaag agcaacttcc gatgtggatg
     3001 ctgtcaggcg gtttttctcc gaacaggcga tcggaatcgg ccggatatgt tttttgtatg
     3061 cagtcaattt catagcaatc tatcggatgg acagctcatt ggctctttca actttgatga
     3121 tcgttccgtt ggtatttctt atggctctgt ttttctttcg aaaaatcggc aagcgctacg
     3181 aagcttatca gaatcaagat gctctattat cgacaatgct gcaggaaaac ctgaccggtg
     3241 tacgggtggt aaaagcgttt gcccggcagg attatgaaat tgaaaaatat cgaaaagaga
     3301 actggaaaaa gtttttactg gggaaaaaac tggtctttac cgaagcttta ttttggcctt
     3361 cgaccgattt gttatgcggg ttccagatgg taatcagcta tatgttgggg gcatatgcga
     3421 cgatttccgg aaggatctcg gtcggagatt ttgtcgcttt tcaggcactg attgtatgga
     3481 ttatatggcc gattcgaaat atgggccggt tgattgttca gatgtcacgt gctttcatca
     3541 gctttcaacg gatcatggag atattcgcaa acgaacagga accgttggat gaggggacgg
     3601 taatgcctcg gaaggtaccg gaaggtgaga ttaattttga tcatgtaagc tttgcatatc
     3661 aaccggagaa cgagaccttg catgatctct catttcatgt ttcccccggt caatcggtag
     3721 ccatcatcgg gccgaccggc agcggaaaaa catcgttgat taatttgctt ccgcgttttt
     3781 atgaatatac ttccggaagg attaccttgg atgggatgga attgaaccgg atcccgcgga
     3841 attatttacg gaaacatatc ggaattgtcg aacaggagcc tttccttttt tcgcgttcca
     3901 ttgcggagaa catcacgtac ggcgccggca gagaagtctc gacggatgag attatacaag
     3961 cggcaaaagc cgcagcgatt catgaaagta tcctttcatt tgaaaagggc tatgacacat
     4021 tggtaggcga gcggggtgta accttatctg gcggtcagaa gcaacggatc gccattgcca
     4081 gaactttgtt gaaaaatccg aaaatccttc ttttggatga tgctacctct tccgtcgata
     4141 tggatacgga agtggaaata agggcggcgt tgaatgaatt aatgaaagat cggacgactt
     4201 tcattatcgc tcatcgaatt cagacgatta tgcaagccga tttgattttg gtaatggatc
     4261 agggaaaaat tcttcagtcg ggtacacacc aagaattggt tcaacaggaa ggattttatc
     4321 ggcaaatatt taatcttcaa tctcaaattg aagaagccct cgaaaaggag ctgtcacatg
     4381 gcggataacc aatttgaaga agaggatttt caatcaaaat tcaacgggaa tgtaataaaa
     4441 cgcatttttg aatttattac tccccataag cgctggttct tcctgttcct attttgcact
     4501 ttaattacca gtgtcatgga ttctctgcag gtttatttga ataaactatt gcttgataat
     4561 gctatcgtcc ccggcttgaa gagtcaggtg ctaccctatc tcaaatatta cggtctgact
     4621 gtaattgttc aagcgatagg ggttttcact tttgtatatc tggccgggat gctttcggaa
     4681 cggatctggt atgacttacg acaaaaaatg ttcgatcatt tgcagaaact gtcgctgtct
     4741 tactatgcgg ttacaccggt cggttggatt atgtccagag tgaattcgga tagttttaaa
     4801 atcgcagatt tggtgacttg ggggatggtt gattcttctt ttgcaattgt caatattttg
     4861 atttccggcg gatttatgtt ggcaatcaat tggaggatgg cattaatagt cttcgctgtt
     4921 tttccgatca tgttgtatgt ttcctggcaa ttccgtaaaa gaatcctaaa ggaatttcga
     4981 ttggttcgaa aattgaattc caaagtgacc ggagcgttta acgagaatat catgggtgta
     5041 agagtggtaa aagcatttca acgcgaagat caaaacctaa aggaattcaa tgatctgact
     5101 tatcccatgt ttcaatcggc ttacaaagca gcacggctga atgcgctttt tctgccttgt
     5161 attcaactga tctcatccgt cgcattggga ttaatcatct ggtcggctga ttggcagatc
     5221 cgctcgggtt tgatgaccat cggcggaatt caggcattcg taaactatgt cacttcgatc
     5281 atgtggccgg tacaggattt ggcaagagtt tacggggaaa tgcagcaatc cgtcgcgtcc
     5341 ggagaaagga tattttcttt gttggatacc caacctgcga ttgtcgataa gccggatgct
     5401 ttcgaaccgc agaccctttg cggagaaatt gaatttgacc atgtcagctt ctggtatgat
     5461 gacggtgacg gcaggccggt cctcgaagac ttttctctca aagtgaatca gggagagaca
     5521 attgctttgg tcggatcgac cggtggagga aaatcaacga ttgtaaatct gttggctcgg
     5581 ttttatgaac cgaaaaaagg cgttattcgc atttgcggca gagattatcg ggattataaa
     5641 atggaaagta ttcaatcccg aatcggaatg gttttacaga ctccgcattt gttttcggga
     5701 acaatccgcg agaatatccg atacgggaaa ttggatgcga cggatgagga aatcgaacag
     5761 gctgcccgtc aggcgggtgc cgatactttt attcgttcat tcccgaatca gtatgaggaa
     5821 caggtggggg aaggcggtaa tttgctttct ttcggacaaa agcaattaat cagcttggca
     5881 cgggcgatat tagccgatcc ggagattttc atcatggacg aagcgaccag ctcagtcgat
     5941 actttaaccg aaaatctcat tcaacagggg atggatcata tgatgaaagg acggaccagt
     6001 tttattatcg ctcaccggct ttccacaata aaacgcgcag accgtatcct ggttatcgaa
     6061 gacggtaaga ttatcgaaga aggtacccat tttcaactgc tggcaaaaaa aggaaaatat
     6121 taccatttat atacgcaaca gtatcgcgaa gaaaaagaac gggatctgaa actgaatccg
     6181 ttatccgcat aagttccttc aaagattcga ttcgaatagg tattgatgtt tttttccgga
     6241 agacaatctc ggcaaatgtc ttaacaatta tgatattaat aagtatattt tcatgaatcg
     6301 cccgcgaatt tttgaggaaa aatgaaggcg aaaagttatt cgattgaaaa tgttagaaaa
     6361 aaaagatgac aaaataatta agaatatgtt gttagacaat agccaaaaaa gttgcaaatt
     6421 ccgaccggaa atgataaact gaaattgatg aaaagatttt catccaataa attccgtaac
     6481 agcatggatc atcatttaat ttataaagga aatgaaaacc ggaagaaaat gatggaattt
     6541 agcgggaaga tcaatgaact tttatcttat taagattacg aattcccgtt agcaattatc
     6601 aaatatttat tttatcaata acgagtcatt gaaaaggaga aaaacgatga gcgatagaat
     6661 gacctgcata cccttttctc aattgttgaa ttgggtcttt gatgaatatc aatcacaggg
     6721 caccgtattt ggggtccatc aaccgtataa agccaaagaa gggaagacgc tggaattgtt
     6781 cggtcgaaag cttgagactc cgatcggacc ggcagcgggt ccccataccc agttggcgca
     6841 gaatatcatt tccgcatatt ttgccggaag ccgctttttc gaagtaaaga ccgttcaaat
     6901 tatcgacggt gaagatttac ccgtatcaaa gccttgcatc gatgccaccg atgagggtta
     6961 caacatcgaa tggtcgacag agcttacggt ccccgatgcg atgggagaat acattaaggc
     7021 ttgggtaata ttgcatttga tggcgaagga gatggggctg ggcgcgcagg atggattcca
     7081 atttaatatg agcgtcgggt atgatctcgc cggcatccga tcggaaaaaa tcaatacttt
     7141 tatcgattca atgattgaag caaaagatac cgaagttttc aagaatgcca ttcgaaccgc
     7201 taaagcaaat ttatccaaat ttaagcatgt gaccgaagct gatatcgatg cgattccgtc
     7261 cgctatttgt aattcggcga cgatatccac tctccacggg tgtccgccca aagaaatcga
     7321 aagtatcgca acctatttaa tcaccgaaaa gcatttgaat acttttgtaa aatgtaaccc
     7381 cacacttttg gggtatgact ttgcccggaa gaccttagat gaaatgggat atgattacgt
     7441 atccttcggt cgattccatt ttgaagatga tcttcaatat caagatgccg ttcccatgtt
     7501 tgaacgattg attgatttag ccgcaagtca ccaccttcag ttcggtgtca aactgaccaa
     7561 tacttttccg gttgatatta cgcggcatga attggccggt aaagaaatgt acatgtccgg
     7621 taaaccgctt tttgcattga caagcgcgct ggcagcgaaa ctttccagag attttgacgg
     7681 aaaattacgg atctcttatt ccgggggtgc ggatgctttc aatatcgaaa aaattgtagg
     7741 cgccggaata tggccggtga cgatggcaac gacaattctg aaaccgggcg gatatcaacg
     7801 atttcgacaa ttagcggaga aattggaaaa gcaagggtac cattcgtttg aaggggtaaa
     7861 tgttgctgcc atctctaaaa tcgccgagga tgcaaagaaa gataaacact atctgaagcc
     7921 ggcgaaaatg ccgccttccc gaaagaataa ccgtaaagtg ccgcttttgg attgttttat
     7981 cgccggttgt gaggaaggat gtccgattca tcaggatatt ccgacctaca ttcgcctgac
     8041 gggagaagga aaatacaaag aggctttgca ggtcattttg gataagaatc ctcttccctt
     8101 tattacgggg actttatgcg cacatccttg tatgacaaat tgtgttagga atcattatga
     8161 atcaccggtc aatattcgcg gcgttaagct cgaggctgcc aagcagggat atgaaagcgt
     8221 tatgcgcgaa ttaaaaccga ccggacattc ggagaaaaat gtcgctatcg taggtggcgg
     8281 tcccgccggt atcgcggcag ctttttatct tttacgcgca ggcgcgaagg ttacgatttt
     8341 tgaaaagcga cctcggttgg gtggaattgt ccgatatgtc attccgcgat tccggatcga
     8401 tcccgcaaca gtcatcaaca aggacttgga tttcattcgg caattaggag tcgaaattca
     8461 tttgaacaca gaagtatctt cggtggaaga tttgaaaacc ggactttttg atgccgcgat
     8521 tatcgccatc ggagctcatg ttccggctca aatcactatc gaaggatgta cgcctctgca
     8581 tgcgctttca ttcttggaag agttcaacaa taccaaaggc aagatgaata tcggaaaaaa
     8641 tgtcgtggtg atcggaggcg ggaatactgc catggatgta gcccgatcgg ccaagcgaac
     8701 cgagggtgtg gaaaatgtat atttggttta tcgcagaaca aaacgatata tgcctgccga
     8761 tgaagaggaa ctgcaattgg cattgaacga cggagtaatt tttaaggaat tgcttgcgcc
     8821 ggaaaaatgg gaaaacggag aattgatttg ccgggtaatg gaactgggcg aatacgatga
     8881 atcggggcgg cgctcggttg tagaaaccga tcaatatgta agggtggctg ccgataccgt
     8941 catcagtgca acgggtgaaa atgtttctac ggatttctat caagcaaacg gaatcgcagt
     9001 ggatgcaaaa ggaaaaccgg ttgtaaatcc gaagactttg gaatccagca aagaaaatat
     9061 ctatattgtc ggggacgggt tatacggtcc ttctacgatt gttcaggcga ttgccaacag
     9121 tatgaaagcc gcttctgcga ttgtcggttt tgatgtcact tccgatgtct tccaacccac
     9181 accgaaagaa agtattttcc cgaagcgcgg cgttttgata aaagaagaag atgccaccca
     9241 tgaaagcgag cgatgtctgt catgctctac cgtttgtgaa aattgtgtag aagtctgtcc
     9301 gaatcgcgca aatgtatcga ttcatgttcc cggaagatcg gaagcacaaa ttattcatgt
     9361 tgattatatg tgtaacgaat gcggaaactg tgagacattt tgtccgtata acagcggacc
     9421 gtacaaagat aaatttactc ttttcgccaa tgaaaaagat atggaaaaca gtaagaatgc
     9481 cggttttgtc gttctggatt caaaagcgaa gaaatttaaa gttcgtttgt ttggcgaaga
     9541 atctgtcatt acccccgaaa gccgcgattt tccggaaggg attatggatt tgatgaattc
     9601 ggtctgtgat aattatccct atttgatttt tgaaaaatag aaatatcact gatgagtgag
     9661 ataaaacggg aaggaatttt ctttcccgtt ttatttttcc aaaggaaaga ttccgaaaaa
     9721 actcatctgt aataatgcaa aaattgcccc ttgcaaaatc aagaagatgt tcttataatt
     9781 tgtttattca tcatcaaacg ggacgctgtc ccgtaaaaca ggacaaaatg caatggctga
     9841 aaaggaaagt tatttacatc gagtatttgc tttattggat tgttttacgg cagaacgtcc
     9901 cgaattggga gtgcgagaag cttcccgtat gttaggaatt tcacggagtg ccaccggcag
     9961 gctgatgagt gaattacgga atgaaggtgt attaacacag gatttggaca cttctttata
    10021 cagtctgggc ggaagagtaa tccgctgggc gggggtctat gaagcggcat cggatttgag
    10081 tgtaaaagca cttccctata tccgtcaatt attaaataaa accaatgaaa cggtaagcct
    10141 gtatgtggtg gaaggcaatg aacgggtctg tatcgaacgc ttggaaagtc gccaaaacgt
    10201 tcgaattgta gaaccgatcg gacaacggtt aaaactatat gccggttcgg gagggcgtgc
    10261 gattttggct tttatgcctc ccgaagaaat taaccgcatt ttgaaattgg ctgagcaaga
    10321 aattacggaa ggagatgttc aggaacaaat tcgggaaatc cgggaacatc ttgaagaagt
    10381 aagaagaacc ggtgtcgcaa tcagtcacgg acagtgggtg gacggcgcat cggggatcgc
    10441 agccccgata tttgatgaaa gcggattacc gatcggttcg gtatctgttt cggggccgac
    10501 aaaccgattt agggatcaaa aaacattgga tgcttatcga gaactgctct tatcaacgat
    10561 ggaaaagtta tccaatgaaa tgggatatcg aagtgatcgg atcattgcga atccataaaa
    10621 cagaaaaaag aggaattcaa atccaaatac aggaggaatg aaatgatatt agaggacaga
    10681 attcaagcag atgtcagtac atggcgagaa agggttaatc ggctgacaaa agaacatggc
    10741 gattttgtga tatccaatgt aaccgtttcg caaattttct cgggaattcg gggagtaccg
    10801 attcaagtga gcgatatatc ttatgtagat cccgaaaagg gaattcgata tcggggatat
    10861 acgattaacg aaattctctc tcttctcccg aagctgccgg acacaaatta tcctttagtc
    10921 gggggtgtct atcatttgtt gatggggaat gagttgccga cgatggaaaa agcgctggcg
    10981 attgagaatg aatggaagaa ccgttcagaa gtgcctgatt atgttttccg aatcattgat
    11041 tcttttcctg aaaatgcttc aacgatggcg atgtattccg ctgcgattct gtccatgtcc
    11101 tccgattcaa aattctcaaa aggctatcat gactccctgc ggaaggaaga ttattggaaa
    11161 gcgatgttgg atgacagttt ggatttgacc gcaagatctc ctcaaatcgc agcatacatt
    11221 tacaacaaaa aatatcgtaa caacgaacag atcggtcccg ataagtcgtt ggattatgcc
    11281 gcaaactttg cacatatgat cggaaaaggt gatgacccgc tttatgccga tttgatgagg
    11341 cttttcattc tgctgcatgc cgatcacgaa aacgccaacg tcagtgctca taccgctcat
    11401 ctggtcggtt cggctttatc cgatatttat ttatcctgtt cggcgggttt aaacggtttg
    11461 gcgggcccgt tacacggtct tgcaaatcag gaatgcttta attggctgct tgccctttac
    11521 aatacctatc agggcgttcc taccaaacaa caagtcaccg agtatattca gaaaacattg
    11581 gatgagggga aagttattcc gggttacggg catgccgtat tgcgttgtat cgatcctcgt
    11641 tttgaagccg aaagacaatt tgcggaaaaa tatttccccg atgatgagta tttccggacc
    11701 gcgatggtcg tatttgaaac agcgcccgat atgctgaaag ccaccggcaa ggtgaaaaat
    11761 ccgtggccga atgtggatgc gttgaatggt acattgcagt atcatttcgg tgtgactcag
    11821 tccgattact atacggtgct gttcggtttg agccgtatgc tcggatttac ctgccacatt
    11881 gtctgggcga gagctttagg aaagccgatc gagcggccga aagcattgac cacacgcttg
    11941 ttggaatcaa tgattgctga agagaagaag caggaagccg ctaaataagc aaaagcagaa
    12001 agaaaaaaca gatatattga aaaatatatc tgtttttttg tgtatagatt gatcgagaaa
    12061 tgcgctattt gcggcgataa aaatatcttc cggaaaagaa gaagaccgtc gtcaataaaa
    12121 taatagaagc aattccgatg aaatatgttt tcgtgcttgc tttcggcaaa tgattcgcag
    12181 cgatcagagt gggagtcgga gtcatactgg gcagaggtgt catcgtcaaa gtttgcgtcg
    12241 gagaaggggt ttctgacgga agaggtgtta aagtcggcgg aactgttaca gtcggagtaa
    12301 ccggaataaa caacttccat ccaactccaa gcgttgtgct ttcatcgata ccgttccagc
    12361 gcattaattc ctgaatcgta gtcccccata atttggcgat tgaagaaagc gtttcccctt
    12421 ccgtgataac atgtcgatat tttccctcac tgtcagcggt cggcaaaacc gtcggtaaaa
    12481 cggtggaagt aggcgcttgc ccggctgatc ctctttcggg tattaacagt ttttgtccga
    12541 ggctgatgga ttcgatgtcg acaatgttgt tccaagtggc aatatcttcg attgtgacac
    12601 ggtacgcggt agcgatcgac cataggcttt gaccgttttc aactacatgn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgctgacatc tccgatatgc caagacatat
    12781 tattctgtgc catgaccaag gcggtgtttt gggcgcatgc catcaatgcg gaatcgacgg
    12841 aaagggctcc caaaccgttt gcagctctca ttccgttaat caaagagatt aaatcagatg
    12901 ccgtcacaga attttcggcg gcataaacgg gctgaatcga attccgtcgc aaaaaatcag
    12961 cccgatcttt gcagttcagg ctgaaaaaaa ggagaaaaac caaaaaaaag aaaaaacttt
    13021 tccgcattca gaattgttga aaaatactct tcacctattt aaccggcgca acatctgtta
    13081 agcgtggcat ttgaataata attaaatcat ccgcatcgct gccgccgttt gcctgtactt
    13141 caaaggtgta ggttaaactt tgatcatgat atccataatc gcttaattgg atattccatt
    13201 ccgtcacata gccatcgtta ttttcttggg tttccgtaac gcgttgttca gtgcttcctt
    13261 ccacctgcac aaataaacga acaaagttgt cacaacccgg tgcattggga ggacaggata
    13321 cggctgcccg taaccatgta ccgcgaggaa ctcgaattgg accgaaagtg actctggttt
    13381 tgccattttc ttttaagggg ggaccgatca ctatggatgg ttgtcccgca taattgctct
    13441 ctactgtcgg tgaagcggaa taatacgcga ctcctctgcc tgatgaacgg tcggatgatc
    13501 cgcagaagaa caaaccgctg tcatccgaac aaatggcacc agccagattt tcataaaagt
    13561 tatattgcga tcccacctta aactttgacg ataagggata cccttccgcg gaatctccac
    13621 ctttaaatat ctgtccgtcg ggcgacttca atgcccaata ggtgctatat tctccctgtt
    13681 tctcgggagc ggtaattatc acggatatga tcgcagattc cccgttttta agaagtttat
    13741 ctgaggtaaa cagcggaaat tcctttgcgg ttccgacatt atctccggac aaataaacaa
    13801 tactgtaata tttggtccat tcacaagtac cggtgttttg gacataccat gatttggtaa
    13861 aagtggtcaa aggcaatgtc attgttccat ccgggaatgt aacatccgat aaaaattcga
    13921 atttattggt acaggccgcg gaaccgcttc cggaaccgag ggaaagagtc ggagtaccgc
    13981 cgatttcggg aaggacgttt tcctgcatat cgagatagac ttcggttgcc aggctactta
    14041 ctttccggtc cacctcggct gcgatttggg tattttgtgt ctgttgggat tgatccaatt
    14101 tcgcttgatc caaaagatcc ttttcttcca aagcggagga gataccggcc tgaatggtgg
    14161 catccaaatt gataggttcc ggtgaatgat aagtacatcc cgataccaaa gaaaggaata
    14221 ttaatccact taaaaacgca aattttttca tttaacctcc actttaaccc aaatcggctt
    14281 atcccggttt gaaccggctc caaaacggat gccggaaggg ctttcgaata accaatatcc
    14341 ggcataggat ccgctttgtt caggggcaat catataaaca gatacttcaa ttgtatcatt
    14401 aggcttaacg gaagtaccga ccggtaaacc ggtccgatcc aatgcggaca ttttatcacc
    14461 gtcgctgaat acaacggaat atgcctcggt ccaggtacaa gtgccggtat tttgaatttt
    14521 ccaggtcttc gtaaagggtt taccggcttt cactacggtt ttgtcgggga ttgtgatatc
    14581 ttcgacaaat ttcaaagaat cgatgcagga agcgggtgta ttttcaacta cgctgttttc
    14641 tttttttctt aaagcctccg gtaccgaagt tccccgaaac ggtgctgcga caacggatgc
    14701 cgtcggagtc ggggtttcgg ttatattttc aatcaggctc ggttgttttg cttccattgt
    14761 cgcgagcttt tggtcaacat caaacattat ctgagtggaa gctttagaga ccgcttcatc
    14821 cactaaccaa tagactgttt tctcgaaatc cggtgtggga gaaggggtcg aaagcacaat
    14881 cgaatcacat ccgcaaatca agaaagcgaa aagaataaag gcaatgattt taagcattag
    14941 cggattgact tgcattttga attatttttt ttctctttcg atttccactc ctgccgattt
    15001 ggcaaatcgg acagcggcgg ttttttccac gcgcactttt accgatcgga ccaacggatg
    15061 ttgaagacaa taatcagcaa tgtcggtcgt cagagcttct aatgtctttc gattattgtc
    15121 ctcgaccaaa gccaatacag ctttggcggt ttcactgtaa tccacacaat ccaataaatt
    15181 atccgatagc ccggcttggt gtatatcggt ttgcatctcg atgttgatca gaatatcctg
    15241 cggacgttct ctttccttgt ccgaaatccc gatccgaccg cgtgccagta aatctttgag
    15301 aataattcga tccaatttat tcctcgatat ttttttccaa ggttatttct ggagttgtca
    15361 tccgacaata atccaattcg tacgtatcat aacgttcgga tgtcatttca ttatcttcca
    15421 aagacgcgac cggttcataa tcattcctgc attttacttc aaagcgatac ccttccgcga
    15481 tagccaaaat cttcaaatcg gtggtcttta aaacaatgtc ggtaccgggt tgaacataat
    15541 cgagtttcgg attaatgctg ttgacatgaa cggagactaa cccctttcca tcttgccata
    15601 agcgcatatt cccatatccg tagatatcac tgtattgttt ggctgttcct tcggcactta
    15661 aatccttaat actcgtaacc ggtccaagca ttgttacatc cagtatataa atattactgt
    15721 caattttttt cccgactgtt ttcaaactgt atccgctgtc gctgatgcgg gtttgacaag
    15781 cgataacgaa aaaagagaaa acgataactc cgatcagaat aaagagtaat atttttttct
    15841 ttggcatttt ttgctccccg tttgattcta ttctattagt ttccgctcga tttaagcgaa
    15901 aaagtgatac aaatctcaaa ataagtttag ctgatttttt gaaaagattg caaacttttt
    15961 caacggaaaa ataagacgcg gacttttatc cggaaggaga ggatgttttc gatttatcgg
    16021 agttgattgt atgcaaataa gaaaatttga ttcgatataa ttaagcttga atatgaatga
    16081 agaaaatcaa atcgggaacg actatagcat tttgacggtt attatcctgc tggtttatgt
    16141 attgagcact tttataacca gctatattgt aacgatcccc attcacatcg gtaatttttc
    16201 tctgattcta aattggaaaa tgattgactt aatcacaccc ggcgcagggt tgctggcagg
    16261 gatcggtgta tttggtttag tcgatgaacg gtcgtcgaaa agcaaagcgg aaaaaatgct
    16321 ccattccgtt atcccgtttg cgacaacggt ttcattggga atcattctga gaaatgttgc
    16381 tatgtctgcg ggttgggtta tgattttgat ccttggcggt gtcctgcttt atttagtgtt
    16441 tatgtccgag tgtatcattt gcaatcccgc tgacccgcga atttcggtgg tttcgatcct
    16501 gttaaccggt ctggcttatg cgacatttgt aattactgcc atcggtgtac gatccaatat
    16561 ttccaggctg attttggaat tcccgttgat tctgctgtct tcatttattc tggcgatgag
    16621 gatcttatct ttgtatgagt ccgttaaaga tatcgaaata aaagccgcga tcatttcgtt
    16681 aattaccgcg gaagcggcgg ccgccctcca ttattggccg attcaacccc tttcttacag
    16741 ccttttattg tttttaggtt tttacatttt aaccaatatc gttataatac ttccacagaa
    16801 gggttccttg cgaaatgctt ttaaaagtca gctttggatc cttgcggtat cgattatatt
    16861 aatggtattt attgaaattc aaaaatagtc tttaaggaat tgaaatgaat tgggaaatcc
    16921 tcatatcttt aatcatgagc tatttgatcg gttcaatccc atttggttgg atcattgttt
    16981 ggattaagaa gcatcaggat attcggtatc atgcgtcagg gaaaatgggc acttcgaatg
    17041 ttatccggat gacggatgtt cccaccggta tcataacctt tttccttgac ctttttaagg
    17101 gatatgccgg tgtttgggtg gtaaggttga ttatggatcc ggctcccgca tggatggaag
    17161 cggtcggcgg ctttttagcc gtgctcggtc atgtttattc gatatttatt gtcgaaaaaa
    17221 ggcgtgacgg caggtactat tttcgcggag gtgccggcgg aatgacatcg atgggggcgg
    17281 cgattgcttt gtggcctccc gtcatccttt acagcggaat tccgagcctt ttgatttatt
    17341 ttatttttgg atatgcctct gtcgccacca tgtcgatcaa tgtcgtggca ttgatttgtt
    17401 ttatcgttcg ggcggcatta gggctggatc cgacttggtg gtatgcggtt tatgcatttt
    17461 tcacgctttt gctggttctg aaagctcttc ggccgaatat cagacgatta cgacgcgggg
    17521 aagagcgcgt gatgggtttc agtat
//
LOCUS       DAOSMM010000049        16965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3401_length_16965_cov_17.633116, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000049 DAOSMM010000000
VERSION     DAOSMM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 16965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16965
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3401_length_16965_cov_17.633116"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..2672)
                     /locus_tag="PK898_07585"
     CDS             complement(141..2672)
                     /locus_tag="PK898_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19932.1"
                     /translation="MEKKYIKGKRTLFWLLLISVILLFTFSASAQVYPDGCFNQYGVE
                     RTSADSNDNDFCYDSMGNLTAVTFLEYQANQAFSGNLNGNPFGQGNGQVVLQLPTAIP
                     TAVPSESPSQKTIMMSVPIIGTVTEIIGKYPAQGQVYQTTDIAFVWSFDSAGVDTVTT
                     SFTLYIDGVGYPVYPTCDVSSCQTTRSVPVGYHYWYVTAQAGTWSVSSSVIYFSVTAP
                     ASAAPAKPILQSPSGTIQSQQTQMVWLPAANATSYTVNWTGPVSGSKSLIGTDESCQN
                     GYCSLAVDSLALGNYSWTVTAYNSTSGLSETSDPMVFTVAAPTSKPGKPILEEPNDTY
                     TSSSVNFIWKPVSDATTYTVYWTSQWGQTGSKTLAAGDVSCMSGDCHVSDIMPAIGSY
                     SWYVIATNSAGSTQSDPMYFTIHLGVTAPTGVSPVGTVNNSQPFNYTFTAVEDNTYEY
                     NVRVFNVYTNAQAADYYWNVSDLNCSGGYCTGTANSTLPSGYYYWKVRARSNNDVSEW
                     SAPVYFNNSYCYNCNQPGYQPNTVPTPYSPVGIIQTPVPVFSWKPITGATAYWLRIYD
                     AAGTMIYSGLTDNSVCDYQTCTYSPNFTLPGNGNYSWEIIGGSNAGVAWNNAYATFTY
                     QGQPIKIVPAKEPAEIRFVYPAEGGKLYTGEAQIIWVDPFETVSSYKVTISDTNGQHL
                     LDAVLDRKSAWCDEKTCTIEFAQIPLNQLYKIEITPVGKSGKEGKKASLNFSVTDQPL
                     EFEALYPMQNQKADRQPFFSWRLPSGTPEDQQRKYTYAIRLIDKTRNRESVLGPFTCE
                     SDGMVCFKGGAFFTLSAPLEAGQYQWGVQVKELQKVSAALTFVIE"
     gene            complement(2850..>5191)
                     /locus_tag="PK898_07590"
     CDS             complement(2850..>5191)
                     /locus_tag="PK898_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19933.1"
                     /translation="EPKEETPTEEITEEPKEETPTEEITEEPKEETPTEEITEEPKEE
                     SPAGSSRMMMMSIPMIGTINGISGVHPAQGEVYKTNEVTFIWEVDSAGVDTVETAFKL
                     YLDGGGHDVTPSCGVTICQVTETVATGNHSWYVTAKAGTLSVSSSAITFSVNPATSAP
                     SKPILKNPTGEVQNLTLLMSWLPVADASEYTLQWTGPGGSAGSGSLVATDRSCKDGLC
                     TLPITFSAQGPYSWTVTATNSAGSAVSDPLTFQISDPVSAPGKPVLEMPDGSYTSSTV
                     NFVWKPAVNAESYTVYWQSQWGHTGTKTLPANDATCLSGDCHISDTMPALGSYTWYVC
                     ATNSAGTTQSNTMSFNIYDKVSTPYGTSPSGTIYSENPLSFTFSRVEDNAYQYNVTVY
                     NAYTGEQVADYYWNESELSCSGNYCTGVADSALGKGYYFWRVRARTNNSVSNYSGAVY
                     FRNTYCENCYPYSYYYPNTVPTPYSPIGLIHEASPVFSWKPITGATAYWLTIFDATGK
                     AIFSSSTDNSICNYQICTYSPAFTLPQDGNYTWKISGGSNTGVVWNYASAAFTYVGAE
                     ETQKSVTGISIIYPMEGGELYAGDAKIVWLDTQIENETYHLVIKDKDGNVLLDVGLDR
                     ESAWCDGKTCSIEFAEIPSDATYHLELTPISESGEEGILTELNFQVTDLPLDFAALYP
                     AENQEVGPTAPFIWQLPSGTPAETQKTLTYSIHLIDQTRSRETVLGPYHCEDSGMICF
                     NGGALYRFEEELPEGDYIWSVRANELEKVSEEIPFKITQ"
     assembly_gap    5192..5291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5292..5533)
                     /locus_tag="PK898_07595"
     CDS             complement(<5292..5533)
                     /locus_tag="PK898_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19934.1"
                     /translation="MRKKNWMGVYSLLLIVILSIFSGAANPILQPTAAVVCYDLEGVQ
                     RNAVNSIDTDLCYDAEQNPMGYTFFDYKGFLMTASSS"
     gene            complement(5687..6106)
                     /locus_tag="PK898_07600"
     CDS             complement(5687..6106)
                     /locus_tag="PK898_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU19935.1"
                     /translation="MTRILFILDGSNFLFGENDADPVQILSDIRNGVFALPFRSDPVK
                     CVEIEGWVVVGLRSRKSMIGYLTQQEINVLELLIRGKTVNQIAVALTLSPETIKYHIE
                     NAKIRMQVETRNELIALYCREKESVQNGQLDPELRKT"
     gene            complement(6242..6316)
                     /locus_tag="PK898_07605"
     tRNA            complement(6242..6316)
                     /locus_tag="PK898_07605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6279..6281),aa:Pro,seq:ggg)
     gene            6499..7233
                     /locus_tag="PK898_07610"
     CDS             6499..7233
                     /locus_tag="PK898_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU19936.1"
                     /translation="MQLIDRACQILTILSYQPEGMSVLELSEKINLAPSTTHRILTSL
                     KENKMVVQDQDSKKYRIGYKILSLCANLNERNTLITAAKPVMKQLSKLIDKTIVLCVM
                     ENTYIMNIACIEKADSNLFLVKIGRELPLFSTSAGRVFCAFMDREKVHSVYESVHEQA
                     LTPYTKTSWDALCSEMDRVKENGYACIDEELQLGIKGYACPIFDINGKVIAALAFLST
                     KASLQDAEPMIRSLKESAKQISTEIG"
     gene            7240..7986
                     /locus_tag="PK898_07615"
     CDS             7240..7986
                     /locus_tag="PK898_07615"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU19937.1"
                     /translation="MESTNTVSIKHLDKIYSNLLKEDTVALKDINLEIKNGEFLSIIG
                     PSGCGKSTLLKVIGGLEKPTRGEIEYLDKENRLGFVFQDSVLLPWKNVRENAEFPLVI
                     QKKQTKENEERLDALLELAGLTEFKTALPRELSGGMKQRVSIVRALSYDAPLLLMDEP
                     FGALDAITRDHMNEELLNIWTQTKKTIVFVTHSIGEAVFLADRVVVMSTRPGTIKEIV
                     PIDLPRPRNSQTRNDPKFGEYTGYLRGIIG"
     gene            7983..8765
                     /locus_tag="PK898_07620"
     CDS             7983..8765
                     /locus_tag="PK898_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19938.1"
                     /translation="MKKENKLLKILKGAGEYAFSIGIFILVWHFYVRWNDVPEYILPS
                     PDKVWVSFVDLFRTGTVWPHLWATTYEVVLGFIIGSLIGMALGYLFTKVEPVKTALMP
                     YLIFLQTAPKIALVPLFVVWFGIGVVSKIILIITMVVFPVLSGMMLGLESIPTDVRNL
                     MKVLKATKWQVFSLVEIRYSLPSLFSSFKIGIVQAVIGAIVAEWMSGKQGLGYILTYA
                     SSTYEVSMLLAGIVLTIILGIVTYEIINIFENKALYWHESKE"
     gene            8780..9574
                     /locus_tag="PK898_07625"
     CDS             8780..9574
                     /locus_tag="PK898_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU19939.1"
                     /translation="MEYTSASQPLVEGKMPHISCGSDEIGKIVLLPGDPDRVGYFSEL
                     CDDFKIVSKNREFTVGTGFYRGLKISVCSTGIGAPSTEIAVIQLIALGAKGLIRIGGT
                     GAIDEQIPCGSMIINTAAMRLGGASEFYAPKEYPAVASFEVIESLKKACMERNQPFTM
                     GICGSVGSFYHGQGRSTPFETNYDEKAALQKYIDLNIINLEMEAETIFTLSSLYHVYA
                     GSICSVHCNRITDQWLLENQPSQKEMCMTALLGAYYLNECYLAGNK"
     gene            9585..10787
                     /locus_tag="PK898_07630"
     CDS             9585..10787
                     /locus_tag="PK898_07630"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU19940.1"
                     /translation="MSKKLLFIVCVFVVLAALTGCSGTTPSATPAVEKSTVTEQAAEP
                     TGAVEKTAETVKPAEIAETAPSEPTKAEEAVPAETSEAAATTAEQPVEKSSKTSELTT
                     VAIQIDGSAVPYYAPLYIAQEKGFFAENGLNVEFYYAAAADIVKNVASGNVEFGFPNA
                     DAVVAARAQGIPIKVVNTTYQQGLGAIIFNSNSGIKSPADLKGKKVAVTSLGSANYFQ
                     LEAAMKSVGMSIDDVQVEIVGTGAILNALTEGQVDAIVFSKLRTIELNYAGFPAAEIP
                     CDQFLPSFGNVLVAGEKLVNEKPEIVQGFNKALIKGIEYVIDGHVEEAVDYSIEKMAP
                     TFAEKRDVVIKIINEVFIPTLWQSDYTKEHGIGASDVKKWQALIDSSKEYGTIKESFD
                     AKDLLVLD"
     gene            10804..11583
                     /locus_tag="PK898_07635"
     CDS             10804..11583
                     /locus_tag="PK898_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU19941.1"
                     /translation="MFYRKHRKVILPLAVLPIVLIIWELYIRIFNVPNYLLPKPYDLF
                     VSLITLFTSGGVWKHIAVTLKEILVGSVIGAAIGLVLGYIMAKVKLIEKMVMPFVIIV
                     QTAPKISLAPLFILWMGLGIESKAALVILVVSFPIMINEVTAIRSIDKNAYDLMKVLK
                     ANRWQVFRHIELPYSMEMLLSGVKLALTQAMTGAVIGEMIGAKAGLGYLLTLGSETYD
                     IKMILNAVILLSVMGLLFYLISEYIERKALYWKELDRSVIG"
     gene            complement(11669..13063)
                     /gene="pepV"
                     /locus_tag="PK898_07640"
     CDS             complement(11669..13063)
                     /gene="pepV"
                     /locus_tag="PK898_07640"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HOU19942.1"
                     /translation="MKEQIRAKFEAYWDEMMADLAALVAVNSVRGEETTEFPFGKEPA
                     RALSIALRQGERMGFAVENVDNYAGTIDLGDAEEMIGVMAHLDIVPAGDGWTDDPFTL
                     TFKNDRIYGRGVVDDKGPVIAALYGMRIIRDLNLPLKKKIRLIVGTNEEQGSGCMKYY
                     AQHRPIPSCGFTPDADYPLIHGEKGNFWTWLSFPAEETPILEISGGNAFNIVPAECVV
                     LLDGSQVSADELKKSIRTEDTEGYEPQFMHDAKGNIKMVVRGHAAHGSTPEKGVNAIV
                     SAAKILCNAFGEKAGKMMHFIAESIGRETDGASLGLKVSDDVSGSLSLNLGRIIYSPE
                     QHRLAIDIRYPVKLSLAEMDANYRKMAEAFGLTYEVTEAAEPHYVPKDSPVVQKLLAV
                     YRQVTGDQTADAFTIGGGTYAKALGKNFVAFGPEFPGSEPMNVHDADEHILKELFLDH
                     CVVCTLGMAALAGE"
     gene            13431..14555
                     /gene="alr"
                     /locus_tag="PK898_07645"
     CDS             13431..14555
                     /gene="alr"
                     /locus_tag="PK898_07645"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOU19943.1"
                     /translation="MEKRYDTWVEIDLNAIRHNIQTIALIAGKPVMAIIKANAYGHGM
                     IPVGKAAIAAGCPFVAVARFGEAQELRRAGITAPILVLGYIHPKDIEEAARESIRATL
                     FSYEQMKQYEEALRGTWKKLPVHVKVDVGMGRLGEQAEDGLRFLQSAASSAVFDVEGL
                     YSHFSKADMLEDDATGLQFSRFDRLIREAEKAGLRPRIVHLANSAGAVFHPEICKYDM
                     VRGGIAIYGLDPSDSARLSPDFQPALSWKAGITSVKTVPAGQEISYCGRYKTQKDNSR
                     IGVIPVGYADGFGRILENRVLVHGRRVPVLGTVCMDQCMIDLNEVPDAMTGDEAVILG
                     SQGDDRISAEELGARWHTINYDVVCGIAERVTRLYTPELS"
     gene            14579..16273
                     /gene="recJ"
                     /locus_tag="PK898_07650"
     CDS             14579..16273
                     /gene="recJ"
                     /locus_tag="PK898_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOU19944.1"
                     /translation="MEPLFEWKLPSKIPLDVLEQLASFPRVQQRLLYNRGIRDSVGAN
                     SYLRRQGSLYDPFLLDQMDLAVKTILQAIDSRMKIAVYGDYDVDGVTASALLIQVLTA
                     LGAEVRGYIPNRFEEGYGVNKEALHTLHEEGIALTITVDCGIRSPEEALFARSLGMEL
                     IITDHHEPGDTIPEAAAVICPKKPSDSYPDKNLAGVGLAYKLAEALLTTHPVAGIAAE
                     DWEDFVAVGTVADMVSLTGENRSLVKSGLQKLRVSSRPCIRALAEVSGIRMADIKAST
                     IGFSFGPRLNAAGRLETAKLAFDLLMSRTLNEARRFAIELNDQNRQRQELTKLTQRQV
                     LEEINPARVPILIFSAKEEFNRGVVGLAASRLAEHFYRPAIVGSINGDEIHASCRSIL
                     EFHITDALDQCADLMVKHGGHAMAAGLTIKKDNWSMFVDKMTTIATQRLQDQPLKPVK
                     QVDMEIPLDYLKENVLEQIAEMEPFGMDNPEPLFLIRNLKVVSSRIIGNDLSHLRLKL
                     ISEDQITVDAVAFRQGWRRNDLPERIDVLGTFEKNTWKDQQTLQLNLVDFRSAVSN"
     gene            complement(16294..>16965)
                     /locus_tag="PK898_07655"
     CDS             complement(16294..>16965)
                     /locus_tag="PK898_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU19945.1"
                     /translation="VIMPVFNEEKTLREIISKVKETGLANEILVVDDGSTDGTKEILK
                     EYQDDPVVRVITSEKNEGKGAAVRKGILNARCEYAIIQDADLEYNPKFYPVLMQPIKD
                     GIADVVYGSRFLGSARRPILFWNMVANKILTFTTNILYNNILSDMETGYKLFKVSMVR
                     EIPLHAHGFDFEPEFTAKVLKRKFRIYEVPIDFTPRYYTEGKKIKAKDGFVALWTLIK
                     YRFVD"
BASE COUNT         4473 a         3995 c         3962 g         4435 t
ORIGIN      
        1 cccgctttcc cgttacggat atttcaacct tttaatctta ccttaaaagg ttccgtttcg
       61 tctttctttg atcggtattt taaatgaaac ggctgcccga aaatttcacc gggcagccgc
      121 tctttcctca agacaaaacg ctattcaata acaaatgtca acgcagcgga gactttttgc
      181 aattctttta cttgaacacc ccactgatat tgacccgctt cgagcggggc ggataaggtg
      241 aagaaggctc cgcctttgaa acataccatt ccgtccgatt cgcaagtaaa cggacccaat
      301 acggattccc tgtttctggt cttgtcgata agtcggatgg cgtaggtata cttccgctgc
      361 tgatcttccg gtgtaccgga cggcaacctc caactgaaga acggttgtcg atccgctttt
      421 tgattttgca tcggataaag cgcttcaaat tccaacggtt gatcggtcac actgaaattc
      481 agcgacgcct ttttcccctc tttgccggat ttaccgaccg gcgtaatctc aattttatat
      541 agctggttga ggggaatctg ggcaaattcg atggtacagg tcttttcgtc acaccacgcg
      601 gattttcgat ccagcacggc atccaacaag tgctgaccgt tggtatccga aatggtcact
      661 ttataagaag agaccgtttc aaacggatcg acccaaatga tttgcgcctc gccggtataa
      721 agctttccgc cttcagcggg atagacaaag cgtatttccg ccggctcttt cgccggaacg
      781 attttgatcg gctgcccctg ataggtgaac gtcgcgtaag cgttgttcca tgccactccg
      841 gcattcgaac cgccgataat ttcccatgaa tagtttccgt taccgggcag cgtaaaattg
      901 ggtgaatagg tacaggtttg ataatcgcaa acggaattat ccgtcaatcc cgaataaatc
      961 atggtaccgg ccgcatcata aattcgcagc caataagcgg tcgccccggt aatgggtttc
     1021 caactgaaaa ccggtaccgg cgtttggata attcccaccg gactgtaagg ggtgggtacc
     1081 gtgttgggct gataaccggg ttggttgcag ttatagcagt aactgttatt gaagtaaacc
     1141 ggtgccgacc attcactgac atcgttattg gaccgcgctc tcactttcca ataataatat
     1201 ccgctcggga gtgtggaatt ggctgtgccc gtacaatatc cgccgctaca attcagatca
     1261 ctcacattcc aataatagtc cgccgcttgc gcgttggtgt aaacgttgaa aactctgacg
     1321 ttgtattcat aggtgttgtc ttctaccgcc gtaaacgtat agttaaacgg ttgactgtta
     1381 ttaacggtac cgaccggcga cactccggtc ggagcggtta ctcccaaatg aatggtgaaa
     1441 tacatcggat cggattgcgt cgaacctgcg gaattggtcg caatcacata ccaactgtaa
     1501 ctgccgatcg ccggcatgat atcggacaca tgacagtcac cgctcataca ggagacatca
     1561 cctgccgcta aggtctttga acccgtttgc ccccattgtg aagtccaata aacggtataa
     1621 gtggtggcat cggaaacagg tttccagata aaattcactg aactggacgt ataggtatca
     1681 ttcggctctt ccaaaatcgg tttcccgggt ttcgacgtcg gagcggcaac cgtaaaaacc
     1741 atcggatcgg aagtttcgga taagcccgaa gtactgttat acgcggttac cgtccagctg
     1801 taattaccca aagccaagga atcaaccgcc aggctgcaat atccgttttg gcaactttca
     1861 tccgttccga tcagtgactt cgagccggag acggggcccg tccagtttac ggtataactg
     1921 gtcgcatttg cggccggcaa ccaaaccatc tgtgtttgtt gggactgaat ggttccggaa
     1981 ggactctgta agatcggttt tgccggagcg gctgaggccg gcgccgtcac gctgaaataa
     2041 ataacggaag aagaaacact ccatgttccc gcttgagcgg ttacatacca atagtgatat
     2101 ccgacaggta ccgaacgcgt ggtctgacag gaagatacat cacatgtagg gtaaaccgga
     2161 taacctacgc catcgatata gagtgtaaaa gaggttgtaa ccgtatcgac gcctgccgag
     2221 tcaaaactcc agacaaaggc gatatcggtg gtttgataaa cctgcccctg agccggatac
     2281 ttgccgataa tttccgttac cgttccgata atgggaacgg acatcataat cgtcttttgt
     2341 gacggagatt cggatggaac ggcagtgggg atcgcggtag gcagttgaag cacaacttgc
     2401 ccgtttccct gaccgaaggg gttaccattc agattgccgg agaacgcttg attcgcctga
     2461 tactcaagga atgtcaccgc ggttaaattc cccatcgaat cataacaaaa atcattgtcg
     2521 tttgaatcgg ctgaggtcct ttccacacca tattggttaa aacatccgtc gggataaacc
     2581 tgagccgatg ccgaaaatgt gaataacaga atgaccgaaa tcaataacag ccaaaaaagg
     2641 gtgcgctttc cttttatata ttttttttcc atatagccct cctggttaaa ttacgaagtc
     2701 cgacggtttt tcgttccctt tgtcaacact ctgacaatca tgaaaacatt gcactattta
     2761 tgccgaaaca tttttctccg gtggattaaa atgcgaaccg gatgcggaag aaatccgtat
     2821 ccggttcggc ggaaagaaga gaattgaatt tattgagtga ttttgaaagg aatctcttcg
     2881 gaaacttttt ccaattcgtt cgctcgaaca ctccagatat aatcgccttc cggaagttct
     2941 tcctcaaacc gataaagagc cccgccgttg aaacagatca ttccggaatc ctcacaatgg
     3001 taaggaccca agacggtttc ccgacttctg gtctgatcga tcaggtgaat ggaataagtg
     3061 agagttttct gagtctcggc aggtgtgccg gaaggcaatt gccagatgaa gggtgcggtc
     3121 ggaccgactt cttgattttc agccggatag agagcggcaa aatccaacgg caggtctgtt
     3181 acctgaaaat tcaattcggt aagaatacct tcctcaccgg actccgaaat aggagtcagt
     3241 tccagatggt acgtcgcatc ggaaggaatt tctgcgaact caatcgagca tgttttcccg
     3301 tcacaccagg cggattcccg atccaaaccg acatccaaca ggacatttcc gtctttatcc
     3361 ttaataacca gatgataggt ttcgttttcg atttgcgtat ctaaccaaac gatctttgca
     3421 tcaccggcat ataattcacc gccttccatc ggataaataa tgctgattcc ggtcacggac
     3481 ttttgagttt cttcggctcc gacatatgta aaggcggctg acgcataatt ccagaccaca
     3541 ccggtgttag agccaccgct gattttccat gtgtagttcc cgtcctgagg aagtgtaaag
     3601 gcgggtgaat aagtacagat ctgatagtta cagatcgaat tgtcggtaga actcgagaat
     3661 atcgcctttc ccgtcgcgtc gaaaatagtc agccagtagg cagtcgcacc cgtaatcggc
     3721 ttccaactga acactggtga agcttcatga atcaatccga tgggactgta gggcgtcgga
     3781 accgtgttgg gataatagta tgagtaagga taacaatttt cacaataggt attgcgaaaa
     3841 tagacggcgc ccgagtaatt actgacggaa ttattcgtcc gcgctctgac ccgccagaag
     3901 taataaccct tgcctaaagc ggaatcggca acaccggtgc aataattccc gctacaactc
     3961 aattcacttt cgttccagta gtaatcggct acctgttccc cggtataggc attatatacg
     4021 gtcacattat actggtaagc gttatcttcc acccggctga aggtgaaact caagggattc
     4081 tcactatata tagtcccgct gggggaagtc ccgtaagggg tggacacttt atcgtatata
     4141 ttgaaactca ttgtattcga ttgtgtcgta cctgcagaat tggtcgcaca cacataccat
     4201 gtataacttc ccaacgccgg catcgtatcc gaaatatggc aatcgccgct gaggcaggtt
     4261 gcatcatttg ccggcagagt ctttgttccg gtatgtcccc attgcgactg ccaatataca
     4321 gtataacttt ccgcattgac ggccggtttc cagacaaaat tgaccgtact cgaggtataa
     4381 gagccgtccg gcatctctaa aacaggtttg ccgggagctg aaacaggatc agaaatttga
     4441 aacgtcaacg gatccgaaac agcggatccg gcggaattcg tcgcggtgac agtccacgaa
     4501 taaggtcctt gtgccgaaaa ggtaatcgga agcgtacaca gaccgtcttt acaactgcga
     4561 tcggtagcga ccaatgatcc ggagccggca gatcccccgg gacccgtcca ttgcaatgta
     4621 tattcgcttg catcggcaac gggtagccag ctcatgagta atgtcaggtt ttgaacttcc
     4681 ccggtcgggt tttttaaaat cggtttcgag ggagcggacg tggcaggatt taccgaaaat
     4741 gtaattgccg atgatgatac gctgagcgtt ccggcttttg ccgttacgta ccaactgtgg
     4801 tttcccgtcg cgaccgtctc ggttacctga caaatcgtca caccgcaaga aggcgtcaca
     4861 tcgtgacccc cgccgtccag atagagctta aaagcggttt ccacggtatc gacaccggcg
     4921 gaatctactt cccaaataaa tgtcacttcg ttggttttat atacttctcc ctgagccgga
     4981 tggacaccgc tgatgccgtt aatggtaccg atcataggaa tcgacatcat catcatccga
     5041 gaagatccgg caggagactc ctctttcggc tcttcggtta tttcttctgt cggtgtttcc
     5101 tcttttggct cctcggttat ttcttctgtc ggtgtttcct ctttcggctc ttcggttatt
     5161 tcttctgtcg gtgtttcctc ttttggctcc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn ngacgatgat gctgtcatca aaaacccctt ataatcgaaa aaggtatacc
     5341 ccatcggatt ctgctctgcg tcatagcaaa gatccgtgtc aatcgaattt acggcgtttc
     5401 gttgaactcc ctcaagatca taacagacca ctgccgcagt cggttgcaaa atcggattgg
     5461 cagcgcctga aaaaatcgat aaaataacga tcaacaaaag ggagtaaact cccatccaat
     5521 tcttttttct cataattccc tccaatttct atacgaatat tgaggggctt tcgttccctt
     5581 aaaaaagttg tttttgttgt tttgttcagt ggggaaaaag tccaaaatcc gtctctcctt
     5641 tgatttcatt atatccaaag cgctgtgatt cgaatctttt gaaaacttaa gtttttctta
     5701 actcagggtc taattggcca ttttgaacgg attccttctc gcgacaatac agcgcgatta
     5761 gttcatttcg tgtttctacc tgcattcgaa ttttcgcatt ttcaatgtga tatttaatcg
     5821 tctcgggact cagcgtcaaa gcgactgcga tctgattgac cgttttcccc cgaatcagca
     5881 gttccagcac gttgatttct tgttgcgtca ggtatccgat catggatttg cgactacgta
     5941 accccactac cacccaacct tcgatttcca cacacttcac cggatcgctt cgaaacggca
     6001 gcgcaaacac accgttccga atatcggaaa ggatttgtac cgggtcggca tcattctcac
     6061 caaacagaaa gttgctccca tcaagaataa acaaaatccg cgtcatggtt cagttccttt
     6121 ggcttcaggt taataataga aaatattttc tattttgatg ttctacaaat atagaggatt
     6181 tcagcgggat tgtcaatatc aaaaagaccg cagaaaaaaa ttctgcggtc tttttgatct
     6241 gtcggggcac tgggatttga acccagggcc tcttgcaccc catgcaagcg cgctagccgg
     6301 gctgcgctat gccccgaaca acgagaatat tataccctga aacatttgtg cgacaagaaa
     6361 tgattttgaa aggtctgata aaaataaaaa attcagagct taaacagatt gacaaatcgt
     6421 caatgaataa gtacaatcac gtttaacatt ccataaaaag aaatcttatt ccgttatacg
     6481 gaatggcagg aatcaaagat gcaattaatc gaccgcgctt gtcaaatcct aactattttg
     6541 agttatcaac cggaaggcat gtccgttctt gaattatccg agaaaatcaa tctggcgccc
     6601 agcacaaccc atcgcatcct gaccagcctg aaagagaaca aaatggttgt tcaggatcag
     6661 gacagtaaga aatatcggat cggttataaa atcttgtctt tatgtgcgaa tctgaacgaa
     6721 agaaacacat tgattacggc cgcgaaacct gtcatgaaac aactttccaa attgatcgac
     6781 aagacgatcg ttctgtgtgt gatggaaaat acctatatta tgaatatcgc ctgcatcgaa
     6841 aaagcggatt cgaatctgtt tctggtaaaa atcggcagag aacttccttt attttccact
     6901 tcagccggca gagttttttg cgcattcatg gatcgggaaa aggtccattc cgtttatgaa
     6961 tccgttcacg aacaagcttt gactccttat acaaaaacat cttgggatgc cttatgttcg
     7021 gagatggatc gggtaaagga aaacgggtat gcctgtatcg acgaagaatt gcaattggga
     7081 atcaaagggt acgcatgccc aatctttgat atcaacggaa aagtcattgc cgcgctggcg
     7141 tttttgagta ccaaagcaag tctgcaagat gcagagccaa tgattcggag tctgaaagaa
     7201 agtgcgaaac aaatatcaac ggaaatcggg taggaatcga tggaatccac aaatacggta
     7261 agtatcaaac atttggacaa aatatacagc aaccttttga aagaggatac ggtcgcgctg
     7321 aaggatatta atcttgaaat caaaaacggt gaatttctct cgattatcgg tccttccggc
     7381 tgcggcaaat ccactctgtt aaaagtcatc ggcggattgg aaaagccgac ccgcggcgaa
     7441 atcgaatacc ttgataagga aaaccgtttg ggttttgtat ttcaggattc ggttctgctg
     7501 ccttggaaga atgtccgcga aaacgccgaa tttccgctgg tgattcaaaa gaaacagacg
     7561 aaagaaaatg aagaacggct ggacgcgtta ttggaactgg cgggtctgac ggaatttaaa
     7621 acagctttgc cgcgagaatt atccggcggc atgaaacaac gggtttcgat cgtccgcgcg
     7681 ctctcctatg atgcaccgct tttgttaatg gatgaaccgt tcggagcttt ggatgcgatt
     7741 acacgcgatc atatgaacga agaattatta aatatttgga cccaaacaaa aaagacgatt
     7801 gtttttgtga ctcacagcat cggagaagcg gtattccttg ccgatcgggt agtcgtcatg
     7861 tctacccgac ccggaacgat taaggaaatc gttccgatcg atttaccgcg gcccagaaat
     7921 tcccagacca ggaacgaccc gaaattcggc gaatataccg gttatttaag aggaattatc
     7981 ggatgaagaa agagaacaaa ctgttaaaaa ttcttaaagg tgcgggggaa tatgcttttt
     8041 caatcggtat attcattctc gtatggcatt tttatgtcag atggaatgat gttcccgaat
     8101 atattttgcc cagtccggat aaagtttggg tgagtttcgt ggatttattc cgcaccggaa
     8161 cggtctggcc ccacctttgg gcaacaacat acgaggtagt cctcggtttt atcatcggca
     8221 gtctgatcgg aatggctctc ggctatctgt tcaccaaagt cgaaccggtc aagaccgcgc
     8281 tgatgccata cctgatcttc cttcagaccg ccccgaaaat cgcgctggta ccgctttttg
     8341 tcgtttggtt cgggatcggt gtggtttcca aaattattct gattattaca atggttgtct
     8401 ttccggtttt atccgggatg atgctggggc tggaatccat tcctacagat gtgcgaaatt
     8461 tgatgaaagt cttaaaagcc accaaatggc aggttttttc tttggtcgag atccggtatt
     8521 ccttgccgag ccttttttcc agtttcaaga tcggaatcgt acaggcggtc atcggtgcga
     8581 tcgtagcgga atggatgtcc ggaaagcagg gcctcggcta tattctcacg tacgcttcct
     8641 caacctatga ggtttccatg ttgttggccg gaatcgtctt gacgatcatc ctgggaatcg
     8701 taacctacga aatcatcaat atttttgaaa acaaagcact ttattggcat gaatctaaag
     8761 aataggaaag caaaaattta tggaatatac atccgcatca caaccgttgg ttgaagggaa
     8821 aatgcctcat atttcctgcg gcagcgatga aatcggcaaa atagtcttat tgcccgggga
     8881 tccggaccgc gtcggttatt tttcggaatt atgtgatgat ttcaaaatcg tctcaaagaa
     8941 tagggagttt accgtcggaa caggctttta tcgtggttta aagatttccg tctgctccac
     9001 gggaatcggc gcgccttcca ccgaaatcgc ggtgatccaa ctgatcgctt tgggcgcgaa
     9061 aggtttgatt cgaatcggcg ggaccggcgc aattgacgag cagatccctt gtggatcgat
     9121 gattatcaat actgctgcaa tgcgtttggg gggcgcctct gagttttatg ctcctaaaga
     9181 atatccggcg gttgcatcgt ttgaggtgat cgaatcattg aaaaaagcct gtatggaaag
     9241 aaatcagccg ttcacaatgg gaatttgcgg atcggtcggt tctttttacc acggtcaggg
     9301 aagaagtaca ccgtttgaaa caaattacga tgaaaaagca gcgctgcaaa aatacatcga
     9361 tctgaatatt atcaatctcg agatggaagc ggaaacaatc tttactttat cttcgctata
     9421 tcatgtttat gccggcagta tttgttccgt tcattgcaat cgaattaccg atcaatggct
     9481 gttggaaaac caaccttcac aaaaggaaat gtgtatgaca gctctgttag gagcttacta
     9541 tctgaatgag tgttacttgg caggaaataa ataaaggaga atttatgagt aaaaaattat
     9601 tgtttattgt ctgtgttttt gtagttcttg cagctcttac gggttgcagc ggtacgactc
     9661 cgtcagccac gccggcggtt gaaaaaagta cggtaacgga acaagccgcg gaaccgaccg
     9721 gtgctgtcga aaaaaccgcc gaaacggtga aaccggccga aattgccgaa acagcaccgt
     9781 ctgaaccgac aaaagcggaa gaagccgttc cggctgaaac ttccgaagcg gccgcaacca
     9841 cggcggagca accggttgaa aagagttcga aaacctctga actgacaacc gtcgcaatcc
     9901 agattgacgg ttcggctgtc ccttactatg ctccgttgta tatcgcacag gaaaaagggt
     9961 tctttgctga aaacggattg aacgtcgaat tttattacgc cgcagcggca gatatcgtaa
    10021 aaaatgtcgc ttccggaaac gtagaattcg gtttcccgaa tgccgatgcc gttgtcgccg
    10081 cgagagcgca gggaattccg attaaagtcg tcaacaccac ttatcaacaa ggcctgggcg
    10141 caattatctt caattcgaat tccggtatta aatctccggc agacttgaaa gggaaaaaag
    10201 tagccgttac cagtctcggc agcgccaatt atttccaatt ggaagcagcc atgaagagcg
    10261 tcggaatgtc aatcgatgac gttcaagtcg aaatcgtcgg cacaggcgcc attttgaacg
    10321 ctttgacgga aggtcaggta gatgccattg tcttttcaaa actcagaacc atcgaattga
    10381 attatgccgg ttttcccgcc gccgaaatcc cttgcgatca attcctgcct tccttcggaa
    10441 atgttcttgt cgcgggagaa aagctcgtca atgaaaaacc cgaaatcgtg caggggttca
    10501 ataaagcgct gatcaaaggg attgaatatg ttattgacgg acatgtggaa gaagccgtag
    10561 attattccat tgaaaagatg gcacctactt tcgccgaaaa acgggatgtt gtgatcaaaa
    10621 tcatcaacga agtatttatc ccgactttat ggcaaagcga ctataccaaa gagcacggaa
    10681 tcggcgcttc cgatgtgaaa aaatggcagg ctttgattga cagttccaaa gaatacggaa
    10741 caatcaaaga atctttcgac gccaaagatt tattggtctt agattaatca aatgagagcg
    10801 ggcatgtttt atcgcaaaca tcggaaagtc atccttcctt tggcggttct cccgatcgtt
    10861 ctgatcatct gggaactgta catcaggata ttcaatgtgc cgaattatct gctgcccaaa
    10921 ccttacgatc tgttcgtttc tctgatcacc ctgttcacct cgggaggagt atggaaacat
    10981 atcgctgtta ccttaaaaga aattttagtc ggatcggtaa tcggagccgc gatcggtttg
    11041 gtgctgggat acattatggc aaaagtgaag ctcattgaaa agatggtgat gccgtttgtg
    11101 atcatcgtgc aaaccgcccc gaaaatatca ctggcacctt tattcatcct ttggatggga
    11161 ttgggcatcg agtccaaagc ggcgttggtg atcctggttg tttcttttcc gatcatgata
    11221 aatgaagtga ccgccatccg ttccatcgac aaaaacgcct atgatctgat gaaggtgctg
    11281 aaagccaacc gctggcaggt ttttcgacat attgagcttc cttactccat ggagatgctg
    11341 ctctccggag tgaaactggc tttgactcaa gccatgaccg gcgccgtgat cggagagatg
    11401 atcggcgcaa aagccggctt gggatattta ttgacgctcg gcagcgagac ttatgatatc
    11461 aagatgatcc tgaatgcggt gattttgttg agtgtgatgg gtctcttgtt ctatctgatt
    11521 tccgaatata tcgaaagaaa agcgttgtat tggaaggaac tcgatcggag tgtcatcggt
    11581 taagattttt ctttcaagaa aaatcagacg agccttgtga aaagaaattc cgcaaggctc
    11641 gtttcttaat gaaaaatccg atttcctttt attctccggc aagcgccgcc ataccgagtg
    11701 tacagacgac acaatgatcc aggaataatt ccttgagaat atgctcatcg gcatcatgga
    11761 cattcatcgg ttccgaaccg gggaattccg gcccgaatgc gacaaaattc tttccgagtg
    11821 ctttcgcata agtcccgccg ccgatcgtaa aagcatccgc tgtctgatcg ccggttacct
    11881 gacgataaac cgccaataat ttttgcacta ccggactgtc cttcggaaca taatgcggct
    11941 ccgccgcttc ggttacctca taggttaaac cgaaggcttc cgccattttc cgataattgg
    12001 catccatttc cgccaaactg agtttgaccg gatagcggat atcaatcgcc agccggtgtt
    12061 gttcggggga atagatgatt ctgccgagat tcaggctcaa actcccggat acatcatcgg
    12121 acacctttaa tcccaatgaa gcgccgtccg tttctctgcc gatcgattcg gctataaaat
    12181 gcatcatttt gccggccttc tccccgaaag cattgcacag gattttagct gcagagacaa
    12241 tcgcgttaac ccctttctcc ggcgtactgc cgtgagcggc gtgtccgcgg acaaccattt
    12301 tgatatttcc ttttgcatca tgcataaact gaggctcgta cccttcggta tcttccgttc
    12361 gtatggattt ttttaattca tccgcagaga cttgcgaacc atccagaaga acaacgcatt
    12421 ccgccggaac aatattaaag gcatttccgc cgctgatttc caaaatagga gtctcttccg
    12481 ccggaaaaga cagccatgtc cagaagtttc ccttttcacc gtgaatcaac ggataatccg
    12541 catccggtgt aaacccgcaa gaggggatgg gccggtgctg cgcataatat ttcatacagc
    12601 cggaaccctg ttcttcgttt gtcccgacaa tcaatcggat ttttttctta agtggcaaat
    12661 tcaaatctcg aataatccgc attccgtaaa gagcggcgat aaccggtcct ttatcatcca
    12721 cgacacctct tccgtagatt cggtcatttt tgaatgtcag cgtaaagggg tcatccgtcc
    12781 aaccgtcacc ggccgggaca atatccagat gagccatcac tccgatcatt tcctccgcat
    12841 cgcccagatc aattgtcccg gcataattat cgacattttc caccgcaaat cccattcgtt
    12901 cgccttgtcg aagggcaatc gataatgccc gggcgggttc tttgccaaaa ggaaattccg
    12961 tcgtttcctc cccgcgaacg ctgttgacgg caaccaatgc cgccagatcc gccatcattt
    13021 catcccaata tgcttcgaac ttagcccgaa tctgctcttt catctctgtc tccttgtagg
    13081 tgtaatatag aaattataaa tagaatttcc ggtgaaagga tcgattccgg cgccgtttga
    13141 tgggatgttt cccaaacgtt ggcaaatttt tattcgattt cccgcaaaaa aaggactgct
    13201 gtttcatcat cggaattact ccctgtcggt tttttccgaa tccccgacgc aatggtaaaa
    13261 tcaaaatatg gattattttg aagaaaatcg attcattcct ttctctgaga atttgaaatc
    13321 tccattccgg cacaattttt tatcttcttt gaacattccg ttccggacgg gtatctgcga
    13381 atccgaatcg gagtgttgca aaaagtttta ttgaatcggg aaagatgaat atggaaaaaa
    13441 gatacgatac atgggttgaa atcgatctga atgcaattcg gcataacatt caaaccattg
    13501 cgctgatcgc cgggaaaccg gtgatggcga ttatcaaagc caatgcgtac gggcatggga
    13561 tgatccctgt cggaaaggcg gctatcgcgg ccggttgtcc gttcgtggct gtcgctcgtt
    13621 tcggcgaagc acaggaattg cgacgggcgg gaattaccgc gcccattctg gttctcggat
    13681 atattcatcc gaaagatatc gaagaagcgg ctcgggaaag tatccgcgca acccttttca
    13741 gctacgaaca aatgaaacaa tatgaagaag cgctgcgcgg gacatggaaa aagttacccg
    13801 ttcacgtcaa agtggatgtc ggtatggggc ggttgggtga gcaggctgaa gacgggcttc
    13861 gttttctcca aagcgccgct tcctccgctg tttttgatgt cgaagggctg tattcccatt
    13921 tctcaaaagc cgatatgctg gaagacgacg ccaccggcct ccaattcagc cgtttcgacc
    13981 ggctgatacg cgaagccgaa aaagccgggt tgcggccccg tatcgtccat ctggcaaatt
    14041 cggcgggagc cgtctttcat ccggaaattt gcaaatatga tatggtaagg ggcggcattg
    14101 cgatctatgg cttggaccct tcggactccg ccagactttc acccgatttt caaccggctt
    14161 tatcctggaa agccggcatt acatccgtca aaactgtacc ggccggacag gaaatcagct
    14221 actgcggacg ctataaaaca caaaaagata actcccgcat cggcgttatt ccggtaggat
    14281 acgcggacgg attcggacga atattggaaa acagggtatt ggttcacggt cgacgggttc
    14341 cggttttggg aacggtttgc atggaccaat gtatgatcga cctcaatgaa gtgcccgacg
    14401 ccatgaccgg cgacgaagcg gtcatcctcg gttcgcaggg tgatgaccgg atcagtgcgg
    14461 aagagttggg agctcgctgg catacgatta attatgatgt ggtttgcggt atcgccgagc
    14521 gggttacccg tctttacacg ccggaactct cttaatccct ttcgggagga aaactttttt
    14581 ggaaccgctt tttgaatgga aattaccctc aaaaattccc ttggatgtgc ttgaacagct
    14641 tgcatctttc ccgcgcgtcc aacaacgttt gctctataat cgcggtatca gagattccgt
    14701 cggagcaaac agctacttac gcaggcaggg ttcactttat gatccgttcc tgctggatca
    14761 aatggatctt gccgtaaaaa ccatccttca ggcaatcgat agccgtatga aaatagcggt
    14821 ttacggcgac tatgacgtgg atggtgtgac ggcatccgcc ctgctgatcc aggttctaac
    14881 cgctctggga gcggaagttc gcggttacat ccccaatcga ttcgaggaag gatacggcgt
    14941 taataaagag gcgctgcata ccttacatga agaaggcatt gcactgacca ttactgtcga
    15001 ctgcggcatt cgctcgcccg aagaagcgct ttttgcccgt tcgctcggaa tggaattgat
    15061 tatcacggat catcatgaac cgggcgacac cattcccgaa gcggcggcgg ttatttgccc
    15121 caaaaagcct tccgattctt atcctgacaa aaatttggcg ggtgtcggat tggcgtataa
    15181 attagcggaa gcattattga caacccatcc ggtcgcaggg attgcggccg aagattggga
    15241 ggattttgtg gctgtcggga cggttgcgga tatggtttca ctgaccggcg aaaatcgcag
    15301 tctcgtcaaa agcggactgc aaaagttgcg ggtttcgtcg cgtccctgca tccgagcttt
    15361 agcggaagtc tcgggtatcc gaatggcgga tatcaaagct tcgacgatcg gattctcatt
    15421 tggcccgcgc ctgaacgccg ccggccggtt agaaacggcc aaactggctt ttgatctatt
    15481 aatgagtcgg acactcaacg aagcgcgcag attcgccatc gagttgaatg atcaaaaccg
    15541 ccaacggcag gaattaacca aactgactca acgacaagtg ttggaagaaa ttaatcccgc
    15601 gcgggtgccc attctgattt tctccgcaaa agaagaattc aaccgcgggg ttgtcggatt
    15661 ggcagcctca aggctggcgg aacatttcta tcgtccggct atcgtcggct cgattaacgg
    15721 cgatgaaatt catgcgtctt gccgcagcat tctggaattc catattaccg atgcgctcga
    15781 tcaatgcgcg gatttaatgg taaagcacgg cggtcatgcc atggctgccg gactgacgat
    15841 aaaaaaagac aactggtcaa tgtttgtcga taagatgaca acgatcgcga cacaacgtct
    15901 gcaagaccaa ccgttgaagc ccgtcaaaca agtcgatatg gaaattccgt tggattatct
    15961 gaaagaaaac gtattggaac aaatcgcaga aatggaaccg tttgggatgg acaatccgga
    16021 accgctcttt ttgatccgaa atctgaaagt cgtatcttcc cggataatcg ggaatgactt
    16081 aagtcatctg cggttgaagc ttatatccga agaccaaatt accgtcgacg ccgtcgcatt
    16141 tcggcagggt tggcgccgaa atgatttgcc ggaacggatc gatgtcttag ggacttttga
    16201 aaaaaatacc tggaaggatc agcaaaccct tcaactcaac ctggttgatt tccggtccgc
    16261 ggtttcgaat tgaaatcgtg aaatccgctc cgattaatcc acgaaccgat atttaatcaa
    16321 tgtccataaa gcgacaaaac catccttggc tttgattttt ttgccttcgg tgtaatatcg
    16381 cggtgtaaaa tcgatcggaa cctcataaat ccgaaatttt cgtttcagaa ccttggcagt
    16441 aaactcgggt tcaaaatcaa aaccatgcgc atgcagcgga atttcacgta ccatagaaac
    16501 cttgaataat ttataaccgg tttccatatc tgaaagaata ttgttatata agatatttgt
    16561 cgtaaaggtc agaatcttat tggctaccat attccagaaa agaatcgggc gacgggcgga
    16621 gcctaaaaaa cgagatccat aaaccacatc cgcaattccg tctttgatcg gctgcatcaa
    16681 taccggataa aacttggggt tgtattctaa atcggcatcc tgaatgatcg catattcgca
    16741 gcgggcgttc aaaatccctt ttcggacggc agcacctttc ccctcgtttt tctcacttgt
    16801 gatcaccctt accaccggat catcttgata ttctttgaga atttccttcg ttccgtccgt
    16861 tgaaccgtca tccacaacca aaatttcatt tgccagaccc gtttctttga ctttggaaat
    16921 aatttcgcga agtgttttct cctcgttaaa taccggcatg atcac
//
LOCUS       DAOSMM010000050        16759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3458_length_16759_cov_21.026760, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000050 DAOSMM010000000
VERSION     DAOSMM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 16759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16759
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_16759_cov_21.026760"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..115
                     /locus_tag="PK898_07660"
     tRNA            45..115
                     /locus_tag="PK898_07660"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Gln,seq:ttg)
     gene            274..1989
                     /locus_tag="PK898_07665"
     CDS             274..1989
                     /locus_tag="PK898_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOU19946.1"
                     /translation="MFLPTTKEELNALGWEKADVILINGDSYIDSPYSGIALLGRILE
                     KAGYKVGIIAQPDTNNLNDISRLGEPALFWGVSAGAVDSMVSNYTALKKPRSRDDFTP
                     GGINNRRPDRASIVYTNLIRRAFKNTKPILLGGIEASLRRISHYDYWTDKIRGSILLD
                     AKADYLLYGMADKSILQFAEALKNGQSPQNIRGLVFASHELPENALVIPSLEEVRSDQ
                     KQFIKAFRIFYENCDPITAHRIAQKHDERYIIQNPPAKTLSQQELDEIYSLPFTREQH
                     PYYEAQGPVKALETTRFSVVTHRGCYGECNFCAIAVHEGRTVQWRSEKSILAEIETIS
                     KLPDFKGIITDLSGPTANMYGFECSKKLAQGACKDKRCLYPKPCLMLKIDHQPSLSLL
                     DKARKIPGIKKVFVASGIRYDMILHDSGCGKSYTQELCNYHVSGQLKVAPEHDSPKVL
                     QLMGKPGKELLLQFKRDFDQINRDTGKKQYLSYYFIAAHPGCTIKDMQGLKTFANEEL
                     HIAPEQTQIFTPTPSTWSTLMYATETNPFTGEHIFVEKDPNQKQRQKDILTQKEQKKT
                     WTKHS"
     gene            1971..2897
                     /locus_tag="PK898_07670"
     CDS             1971..2897
                     /locus_tag="PK898_07670"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU19947.1"
                     /translation="MDKTLVIYNRYACKGKFKYSIQDVEKGLKAEEIIADMVLTEYPE
                     HAIDLVEKSVKEGYRQVIIVGGDGTINEAVNGLIQAQKKGYGKATLGVIQNGRGNDFA
                     FGMKIPNELKEAISLIKKAQPIPMDVGYVKTNEKSRYFCNGAGIGFDSAINYRATMSH
                     LTGFPSYFFGLIGSLIKDFKQYNAKISWADGETEMMILLLTTMNGTREGGGFLLAPDY
                     DFQDGKLNAVLIGNGKPLHQLLPLIPRFFTGNLDHPDILRIKTPWLKAEFSTTGLYGQ
                     ADGEIICSAGTEFYAEICPEKIDIISGLPFRK"
     gene            2942..3925
                     /locus_tag="PK898_07675"
     CDS             2942..3925
                     /locus_tag="PK898_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU19948.1"
                     /translation="MEKLKFGILGSAGIAWKQFIPAIALAKNAEIIAVASRSEEKSKD
                     FAEKYHIPLSFGSYEDLLHSKEIQAVYIPLPNRLHKEWAMKAVNAGKHVLCEKPLAIT
                     PEDCLEMGREAEKNHVLLMEAFMYRFHPLNEQIIRNVQSGILGGLETIQACHSFLLKD
                     PNNIRLNAELAGGSLMDVGCYCVNVTRTLFNEEPEAVAAVAHYHPNGVDDQMNILAKF
                     KSGRSAQLSCGLTSDYPSGYTISGRKGYIHAHYGFHLGVSNGTVTQKIDGIESILKDP
                     SNEYTRMIEHFSDCVLNQKPLRYTAAEAAGNMAFIQAALKSAENNGEWTEV"
     gene            3953..5968
                     /locus_tag="PK898_07680"
     CDS             3953..5968
                     /locus_tag="PK898_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19949.1"
                     /translation="MINRIGKGISDHPVRSFIFASGMIAFLAYGLLIPQLGMYGDDPN
                     FLWAYHRGGPNEYKEFMGWIREYGYLIYSFLSPVFGESIISWRIATLVLRWGSSILFF
                     LVFNNTFPNFQKIVVPLSISFLLYPGFMEQAIPVEFLLHFFSLDLILVSLLFTYYGIK
                     HGKGWKIFLFLSVLSEIFGLFICEYFAGLELFRPIIIWQTIHQIPQISAQSVKNKIKK
                     TGLFAFPYLLIFGFFLYWRAFLSSSTYMSQIVTVRLQDNPAMFIGNLMRQILSDFKVA
                     VGDAWLQIFQIPSPGKIRWVYLAIFSLVGIVTWLLIVSTGKKEEKPSDQKLWEFLLIG
                     IFLFLTGGIPVWAAGLKISLTLYGDRLSLSFMAGACIIVMALLALILRQKYFPVLLAI
                     LIAFMAGYQFQLQNQFRKEWDLAKNYFWQLAWRAPDISPNTLVLGDQFPFRSLTDNSL
                     NALLNWNYESPEYPDRELYKFFQISAREGMIDPLPANSPLSHNDFRGNISDTILLYAT
                     PTTCLKVLTTADRNLPFLNIAMKKNLWRSKPERIIPNPTRTVTFQSFMGTEPFHGWCY
                     YFEKGELAAQQNQWKEVVEIGKEAESAGLIPSTMIEMKSFFFAALSENDLETAEHWKN
                     LILTEKGNANYFLYQIKEFQNQSHLSKEAELILDQIQEAFQRELESL"
     gene            6182..7180
                     /locus_tag="PK898_07685"
     CDS             6182..7180
                     /locus_tag="PK898_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU19950.1"
                     /translation="MEKIKFGILGTAGIAKLRFIPAIRNAKNAEVSAVASRNSEKSRR
                     YAEENGIETAYGSYEELLAQKDIDAVYIPQPNGLHKEWSLKAIAAGKHVLCEKPLAPT
                     PEDCLEMKQAAEAKNVVLMEAFMYRFHPLTEFVISQVHSGALGEIESIQASFTFILAD
                     MEDVRWDQKLAGGAVMDVGCYGINASRTFFAEEPAAVSAIAHYRKPGIDDRMGIFARF
                     PSGKQAQIICGFTSDAPIGYTINGTKGWITAYNGFQTGVLNGTITQEISGVRSTFWDS
                     TNEYTRMIEHFSDCVINHKPVRYDAMDAARNMAFINAAQKSAENDGEWVKVKEIKS"
     gene            complement(7184..8401)
                     /locus_tag="PK898_07690"
     CDS             complement(7184..8401)
                     /locus_tag="PK898_07690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU19951.1"
                     /translation="MKILILADGRSPITLNWLRSLKSLNHQIVLVSSYPCEQPQEADE
                     FFILPVAFSGLSGSSIPKNKAKDGSKSEGSDRTFIRRFRPLLINFRYILGPFSLPLTA
                     RKFNRIVSQTNPDLIHALRIPYEGMLLSFYRGNIPCAVSIWGNDITLHAQGSAMMRQL
                     TQKTLKKVRGLAADTNRDIRLGKNWGFPTDEPALVVPGNGGIDFTKVRITAEKEQVGL
                     NWLPENWNIVINPRGFRPGSVRNDTFFQSIPLILRQFPNTLFLCCSMAGQPEADAWLD
                     ELQIHRNTMLLPTIPQELLWSLFSRAQVTVSISEHDGTPNSLLEAMTCGCFPVAGDIE
                     SIREWITPGVNGLLVDPNNPEQLASAVISALLNEKLRSDAAEINRQIILERADSEIVR
                     TEIEVFYNQFVSD"
     gene            complement(8449..9531)
                     /locus_tag="PK898_07695"
     CDS             complement(8449..9531)
                     /locus_tag="PK898_07695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU19952.1"
                     /translation="MKIGIVQRVLAEYRVPLFDLLAESYDGNVSVFAGDPRKDEMIDT
                     SRKLEKAHFYHANNKHLFHGKFYLCFQTNIFQWLRSQNPDVLIVEANNRYPLTRWAIH
                     WMHRQNKPVIGWGLGAGFSQNRLKRRFFRQFDAVLTYSGAGAESYRKMGVSPEKIFIA
                     KNAVTERPKRDLPPERSPEISDHPVILFVGRLQERKRVDLLIEACAALKDSVSPILWI
                     VGDGPARQQITELAAKIFPQTLFFGALYGQELTEKFDQADLFVLPGTGGLALQQAMAS
                     GLPVIAAEADGTQTDLVREENGILVKPGDLKMLTDAIRTLLSDPSRLRKMGDRSFQIV
                     RNEINLENMAESFEQAISFAVRNRKE"
     gene            complement(9528..12098)
                     /locus_tag="PK898_07700"
     CDS             complement(9528..12098)
                     /locus_tag="PK898_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOU19953.1"
                     /translation="MIPRLLKISGFLSYKEPVEIDFTRIHVACISGSNGAGKSALLDA
                     MTWALFGQARKNDETIINNSRNIDAAEVTFEFEYESNIYRIIRCLRRGKTSTVDLLIW
                     NQETGNWHPMTEKSVRQTNKRIEELLHLDFETFINASFFLQGKADQFTSQSAGDRKKI
                     LSNILELDIWEKYQTAAAEKRRNQTVRLSFLENRLDEIEKELSEEPRRREVLNSLDEE
                     LKKSEEQLVIKQQAWQNAKSAETLLESHQAAYQSLLQNYEKSARQIDAQKSRLSKRTL
                     ESLSIQELLKEEPEIESGYRQLQKIREELSEWNKKALENYRIDGERKEITGKIGQLRA
                     GLESEQATLRQEILRLEKNFELIPELSQKQKELQTQLESIEAFLSEKESVNESLRNIT
                     EIIVEKRAENQQLDAQMRDLKKRITELQAAQVGTPCPFCGRELDEEHTLTYLQSMQLD
                     GKKLGDRYRINSEELKKLNEEKDALNRKLSEIQNAEKKHLELSRLLAPISAEIKKIQE
                     NEPYRQKLSDRLNEIQKLLETESFCAEERQRLSVLDEQAKALGYNPAAHEQCREKESE
                     LALNENRWQEVQQAKAALQPIQREIIDLTNQIAADEKELKQIEGSVEQQKAALDEFSA
                     NLPDLQKAKKEFDTVQAETNRIRLEKGAAEQLVSVLESLKKQKQEVIREREEANLQIS
                     QLKYLEKAFSKNGIPAILIDQALPEIEEHANLILGKLTDERMSVRFSTQKEYKDKNRE
                     DKIETLAIFISDETGSREYELFSGGEAFRINFAIRLALSHILTKRAGARLQMLVIDEG
                     FGSQDEEGRQRLIEAIIAVQDDYKKILVITHLDELKDAFPSRIEVEKTPDGSRVNVIE
                     "
     gene            complement(12139..13479)
                     /locus_tag="PK898_07705"
     CDS             complement(12139..13479)
                     /locus_tag="PK898_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU19954.1"
                     /translation="MSQPLSSFLLLVVLIIINGIFSMTEMAIVSVRKARLQHEMEKGN
                     RKARTALELYENPNDFFSTIQIFITLVGVITGAVGASAFSGPVANLLAKVPLFSSIAN
                     TLALLLVSIVITYFSLVIGELIPKRLAISFPEKISLNMSGIMRVLSRITKPLVSFLSW
                     STEIGIKILGVKKTEDDSVSEDEVKVMIEQGTLSGVFDETEQDIVESVFRMSDKTVDG
                     MMTPRTELCWIDLDEPLEESLAEIKESDDSYFPLVRGTTDNVVGIVSAKKILDAYISG
                     DEIDLSKMAETPLFIPESKPALSVVDDLRESGKQVAIVLDEYGGFSGMVTLLDILEEL
                     VGDVPGLYDTYHPEIVEREDGSWLIDGQMDIEEFKDELGIKQLADEDRVGYQTLAGFM
                     LSKFGFIPKAGDSFEADGYRFEIVDMDNRRIDKVMVTKLASPSEDDSDKTDSEE"
     gene            complement(13523..14776)
                     /locus_tag="PK898_07710"
     CDS             complement(13523..14776)
                     /locus_tag="PK898_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOU19955.1"
                     /translation="MSIKVLHFADAHIDIITGGKRDPKSGFSYRTLDFLKALDRIVDS
                     AIDEKVDLVLFAGDAYRDAVPVPTFQREWGKRMMRLSEAKIPVLLIAGNHDSSTASGR
                     ASALQEYDTLSVPYLHLAKTISLWTPEMLDGVPVQVIAVPWIPRSRLVAATQSIGKET
                     ESTGQDLAYQISTEINRILDETDPSLPIILMAHYSITGALFSEFQMAALGQEVLLPEG
                     VMKDRRISYTALGHIHKFQDLNAGNQPPVVYPGSIERVNFGECNDKKGFVIAQIDNRH
                     SSYTFRELEGRNFFSNEITIQNAETFQQEALAALPEADQIPDAMIRLIIHYPREWENF
                     LDERELRKAAESALAFHLVLRPISSPRIRLSEDAAVSSLSHLELLERYCESNKYDKNA
                     LPGLLNLAKQIFNDVDMGISEGSET"
     gene            complement(14773..16113)
                     /locus_tag="PK898_07715"
     CDS             complement(14773..16113)
                     /locus_tag="PK898_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOU19956.1"
                     /translation="MKNDRDYRPIILFGIILFFILFVGLFTPVRDKISFRVENLKNRL
                     YMLSNSPADTVFVPQGGEENISMLSQTAEGTHTPTITMTMTVPPTETAVSVTPAATAT
                     PTLKPTATITPTPLPESAHIDGVKYEDQHGIWNYCAPTNLSMALTFWGWKGNRLDAGK
                     WLKPFDKDKNVMFYEMENFVAEQTQLRSIVRSGGTPELLKKLIAAGFPILIEKGSFMQ
                     EVSGAYSWMGHYNVITGYNDATQIWTVQDSYYKPNYEVTYETLYKEWKCFNFEFMIVY
                     PGDKEAELYSLLGPYVNSDWAKQNAFEMANNQVAEAQDDESRFYAYFNRGTSQVALTD
                     YYGAAQSYDMAFSYYANLDTKTRPYRIVWYQTGPYYAYYYSGRYQDVINLANITLAST
                     NEPYLEESYYWRAMAEAALGEFESAQADVQSCLDIHPGFVACSSLSAEIGMGGS"
     gene            complement(16199..16543)
                     /locus_tag="PK898_07720"
     CDS             complement(16199..16543)
                     /locus_tag="PK898_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19957.1"
                     /translation="MNNKITIIEGPTPIFEATNSDWTAAIVESPANYEVVMTNLRTLN
                     GPGLIERCQSTWEQQDTMYLQFRNSIGLEQEVPIVAARCLDTEEGQKLVLWLRIKPEQ
                     IKLEVHLDEDED"
BASE COUNT         4657 a         3718 c         3515 g         4869 t
ORIGIN      
        1 gtttcatgat aaaatgttct tcgcttgtta tatcaagcaa gcattgggat atggtgcaac
       61 ggcagcacac cagactttga atctgtggat cttggttcga atccaggtat cccagctttt
      121 atttccacag aaagaaatgt tgaattaacg ttcataatcg attgggaaga ttgtccgttt
      181 ttctgaaaaa acggctgtcc ttatttcctt tgactgataa aatcaaatag aagaataatt
      241 tttatataga agaaaccgaa aggttcagtt tgaatgttct tacccacaac caaagaagag
      301 ttgaacgcgc tcggatggga aaaagcggat gtcatcctca tcaacggaga ctcatatatc
      361 gattcaccct acagcggaat tgcgctgctc ggtcgtattc tcgaaaaagc cggctataaa
      421 gtcgggatta ttgctcagcc cgatacaaat aatttaaatg atatctcccg tctcggcgaa
      481 cccgccttgt tctggggagt ttctgcgggc gccgttgatt cgatggtctc caattatacg
      541 gccttgaaaa agccgcgcag ccgcgatgac ttcactccgg gcggaataaa caatcgcagg
      601 ccggatcgag catcaatcgt ttatacaaat ttgatacgca gagcttttaa aaataccaaa
      661 cccattcttt tgggaggtat cgaagccagt ctgcgtcgta tttctcacta tgattattgg
      721 actgataaga ttagaggttc tattctgttg gatgcgaaag cggactacct tttatatgga
      781 atggcggaca aatcgattct ccaattcgct gaggctttaa aaaacggaca atcaccgcaa
      841 aatattcggg gattggtctt tgcttctcat gaattaccgg agaatgctct tgtcatccct
      901 tcgttggaag aagttcgttc ggatcaaaag cagtttatta aagctttccg cattttttat
      961 gaaaattgtg atccgataac cgctcatcga attgctcaaa agcatgatga gcgttacatt
     1021 attcaaaatc cgcccgcaaa aacgctttca caacaagagt tggatgaaat atattctttg
     1081 ccgttcactc gagaacaaca cccttattat gaagctcaag gaccggtcaa agccttagaa
     1141 accacgcgat tttctgtcgt aactcatcgc ggttgttacg gagaatgcaa tttttgtgcg
     1201 attgcagttc atgaagggcg aactgttcaa tggcgttccg agaaatccat cttagctgaa
     1261 atagagacaa tcagtaaact tcctgatttc aaaggaatca tcaccgattt aagcggaccg
     1321 acagccaata tgtatggttt tgaatgttcg aagaaactgg ctcaaggtgc ctgtaaagac
     1381 aaacgttgcc tttatcccaa accttgcctg atgctgaaaa ttgatcatca gccttctctt
     1441 tctcttctgg acaaagccag aaaaattccg ggaatcaaaa aggtttttgt cgcatccgga
     1501 attcgatacg atatgattct acatgattcg ggatgcggaa agagttatac ccaagaactt
     1561 tgcaattacc atgtatcggg gcagctgaaa gtagcgcccg aacacgacag cccgaaagta
     1621 ttacaactga tgggaaagcc gggaaaagag ttgttgctgc aatttaaacg ggattttgat
     1681 cagattaacc gagacaccgg caaaaaacag tatctcagtt attactttat tgccgcacat
     1741 ccgggttgta cgattaaaga tatgcaaggg ttgaaaactt ttgcgaatga agaattgcat
     1801 atcgctcccg aacaaactca aattttcaca ccgaccccat ccacctggtc aacattaatg
     1861 tatgctaccg agacgaatcc atttacgggt gaacatattt ttgttgaaaa agatcctaat
     1921 caaaagcaga gacaaaaaga tatccttacg caaaaggaac aaaaaaagac atggacaaaa
     1981 cactcgtaat ttataaccga tatgcttgca aaggaaaatt taaatactct attcaagatg
     2041 ttgaaaaagg attaaaagca gaggaaatta ttgcggatat ggttttaacc gaatatccgg
     2101 aacatgctat tgacttagtt gaaaaaagtg tcaaagaagg ttatcgtcaa gtgatcatcg
     2161 tcggtggaga cgggacaatc aacgaagctg taaacggatt aattcaagcg caaaagaaag
     2221 gctatgggaa agcgactctg ggcgtcattc aaaacggcag aggaaatgat tttgcattcg
     2281 gaatgaaaat tccaaacgaa ttgaaggaag caatatccct gataaaaaaa gcgcaaccga
     2341 ttccgatgga tgtcggttat gtaaaaacca atgaaaaaag tcgatatttt tgtaacggtg
     2401 caggaatcgg ttttgattcc gcgataaatt accgggctac catgtctcat ctcacaggat
     2461 ttccttccta tttcttcgga ttaatcggtt ctctgataaa agatttcaaa caatataacg
     2521 ctaaaatcag ttgggcggac ggcgaaacgg agatgatgat cttgttgctg acgaccatga
     2581 acggtacccg tgaaggaggc ggcttcctgt tagctccgga ttatgatttt caagacggaa
     2641 aattgaatgc cgtcttaatc ggaaacggga aacctttgca tcagttgctg ccgcttattc
     2701 ccagattttt taccgggaat ttggatcatc ccgatattct cagaattaag acaccctggt
     2761 taaaagcgga attctcgacg acaggtttat acgggcaggc ggacggtgaa attatctgtt
     2821 ctgcggggac ggagttttat gcggaaatct gtcccgaaaa aattgatatc atcagcggtc
     2881 ttccatttcg gaaataggca gtctgttata aaatttcatc aatcatcaaa ggagtgaaaa
     2941 aatggaaaaa ttaaaattcg gaatattggg tagcgcaggg attgcttgga aacaatttat
     3001 tcccgccatt gctttagcaa aaaatgccga aataatagcg gttgccagtc gcagcgaaga
     3061 aaaatcaaaa gattttgcgg aaaaatatca tattccgctt tcatttggat cttatgaaga
     3121 tttactacac tcaaaagaga ttcaagcggt atatattcct cttcccaacc gtctgcataa
     3181 agaatgggca atgaaggcgg tcaatgccgg aaagcatgtc ctttgtgaaa aacccttggc
     3241 gatcactccc gaagattgtc tggaaatggg cagagaagct gaaaaaaacc atgtattgct
     3301 catggaagca tttatgtatc gctttcatcc gttgaatgaa cagattattc ggaatgttca
     3361 atccggaatt ttaggcggtt tggaaacgat ccaagcctgc cattctttcc ttttgaaaga
     3421 cccgaataat attcgcttga acgctgaatt agccggtggt tctctgatgg atgtcggctg
     3481 ctactgtgtc aatgttaccc gaactctgtt taatgaagaa ccggaagcgg ttgcggcagt
     3541 agcccattat caccccaacg gagtggacga tcagatgaat attttagcca aattcaaaag
     3601 cggacgctca gctcaactga gctgtggttt gacttcggat tatccctccg gttacacaat
     3661 cagcggacgg aaaggctata ttcatgccca ttatggattc caccttggtg tttcgaacgg
     3721 aacggtgacc caaaaaatcg acggaattga atcaatctta aaagatccgt cgaatgaata
     3781 tacacgaatg atcgagcatt tctccgactg tgttttaaat caaaagccat tacgatatac
     3841 ggcggcggaa gcagccggta atatggcttt tatacaagcc gcattgaaat ctgccgaaaa
     3901 taacggagaa tggactgaag tctgagaaat acttttgaca atcaggtgtc tgatgattaa
     3961 tcgaatagga aaaggaatat cggatcatcc tgtccgatca tttatttttg catccggaat
     4021 gatagccttc ctcgcatatg gattgctgat tcctcaactg gggatgtatg gggatgatcc
     4081 gaatttcctg tgggcgtatc accggggtgg tccaaacgaa tataaagaat ttatgggatg
     4141 gattcgtgaa tatgggtatc tcatctattc ctttctgtca cctgtttttg gagaatccat
     4201 catttcttgg cgaatcgcca ctctggttct tcgttggggt tcttcaattc tttttttcct
     4261 ggtttttaac aatactttcc cgaactttca gaaaatagtc gttccgctca gtatctcttt
     4321 cctgctttat ccgggattta tggaacaggc gatacctgtt gagttcctgt tacatttttt
     4381 ctcattggat ttgattcttg tttcactttt atttacttat tatggaataa agcacggaaa
     4441 aggatggaaa atctttttat ttttatctgt tttatcggaa attttcggtc tttttatttg
     4501 cgaatatttt gccggattgg aattatttcg gccaataatt atttggcaga cgattcatca
     4561 aattcctcaa atatcggcac aatccgtaaa aaataaaatc aaaaaaaccg gactattcgc
     4621 gttcccttat ctgctcatct ttggattttt tctgtactgg cgcgcatttc tttcttccag
     4681 tacctacatg agccaaattg taacagttcg tctgcaggat aatccggcaa tgttcatagg
     4741 gaatctcatg cgacaaattt tatcggattt taaagtcgct gttggagatg cctggcttca
     4801 gattttccaa attccctctc cgggaaagat tcgatgggtc tatctcgcga ttttttctct
     4861 cgtcggcata gtaacctggt tgctgatcgt ttctaccggt aagaaggaag aaaaaccttc
     4921 cgatcaaaaa ttgtgggagt ttttgctgat cggaatattt ctttttctga caggagggat
     4981 tcccgtttgg gctgcgggat taaaaattag tttgacttta tacggagatc gcttatctct
     5041 atcatttatg gcaggcgcat gtatcattgt gatggcgctg ttggctttga ttctccgtca
     5101 aaaatacttc cctgttctgt tggcgatact gatcgcattc atggcgggtt atcaattcca
     5161 gcttcaaaat caattcagaa aagaatggga tctcgctaaa aattattttt ggcagcttgc
     5221 gtggcgggct ccggatatca gtccgaatac gcttgtttta ggcgatcaat tcccgttccg
     5281 gtctcttacg gataactcat tgaatgcttt actcaattgg aactatgaat cgccggaata
     5341 tccggatagg gagctatata aatttttcca aatctctgcc cgggaaggaa tgatcgatcc
     5401 gcttccggca aattcacctc tttcacataa tgactttcgc ggaaatattt ccgatactat
     5461 cctactttat gcgactccca ccacttgttt gaaagtattg acaaccgcgg atcgaaattt
     5521 gccgtttctg aacattgcaa tgaagaaaaa tctttggcga tccaagcctg aaaggattat
     5581 tcccaatccg acccgaacag tcacttttca atcttttatg gggactgaac cctttcacgg
     5641 atggtgttac tattttgaaa aaggtgaatt ggccgctcaa cagaatcaat ggaaggaagt
     5701 tgttgaaatc ggaaaagaag ctgaaagcgc cggattgatt ccatccacaa tgattgaaat
     5761 gaaatcattt ttctttgcgg ctttatcgga aaatgatttg gaaacagcgg aacattggaa
     5821 aaatctgatt ctgactgaaa aaggaaatgc aaattatttt ctataccaga tcaaagaatt
     5881 tcaaaatcaa agccaccttt cgaaagaggc tgaattgatt ttggatcaaa ttcaagaagc
     5941 ttttcaaagg gaattggaat ctctgtaata caaatcaaca tttttctcag cggcgtgagg
     6001 ggcataagca gtttggatca atctccttaa atttatcggg gatttttctt gagattgatt
     6061 aacaaacatg ccggcttatc cttaagcccg tcaatgctga gaccttattc ttgttgttga
     6121 gtcatgtcca ggcattcttt agtatacttt taacaatcga ttgaataaaa aagaggaact
     6181 tatggaaaag ataaaattcg gaattttagg gacagccgga attgccaaat tacgatttat
     6241 cccggctatt cgaaatgcaa aaaacgctga agtgtcagct gtcgccagtc gaaattcaga
     6301 aaagtcgaga cgatatgccg aagaaaacgg aatcgaaacc gcctatggga gttatgaaga
     6361 acttttggct cagaaagata ttgatgccgt atatattccc caaccgaacg ggttgcataa
     6421 ggaatggtca ttaaaagcaa ttgcggcggg aaagcacgtt ctttgtgaaa aacccttagc
     6481 tcctactccg gaagattgtt tggaaatgaa gcaggcagct gaagcaaaaa acgtcgttct
     6541 catggaagct tttatgtatc ggtttcatcc cttaacggaa tttgtgattt cacaggttca
     6601 ttcaggcgct ttgggagaaa tcgaatccat tcaagcaagt ttcacattta ttcttgccga
     6661 tatggaagac gtaagatggg atcaaaaact tgccggcggg gcggttatgg acgtaggctg
     6721 ctacggaatc aatgccagcc ggacattctt cgccgaagaa cccgctgccg tttctgcgat
     6781 tgcccattat cgaaagcccg gtatcgatga ccggatggga atttttgccc gatttccttc
     6841 gggaaagcaa gctcagatca tttgcgggtt cacttcggat gctccgatcg gatacactat
     6901 taatgggacc aaaggctgga ttaccgctta taacggtttc cagacggggg tattaaacgg
     6961 tacgataacg caggaaatct cgggcgtccg ttccacgttt tgggacagta ctaacgaata
     7021 tacccgaatg atcgaacatt tttcggattg tgttatcaac cataaaccgg tccgttatga
     7081 tgctatggat gcagcccgga atatggcatt tatcaatgcc gctcagaaat cagccgaaaa
     7141 cgacggagaa tgggtaaaag tcaaagagat taaaagttaa tagctaatca cttacaaatt
     7201 gattatagaa aacttcgatt tcggttcgca caatctccga atcagcccgt tccaaaatga
     7261 tttgtcgatt tatttcagcg gcatcggatc gaagtttttc atttaacaaa gcactgatca
     7321 cggcggaagc caactgttcc ggattattcg gatctaccaa caatccgttt actcccggcg
     7381 taatccattc acgaatagat tcgatatccc cggccacagg aaaacatccg caagtcatcg
     7441 cttcaagcaa agagttgggt gtcccgtcat gctcgctgat gctaaccgtc acttgcgccc
     7501 gagaaaaaag tgaccacaaa agctcttgcg gaatcgtagg cagaagcatc gtattacgat
     7561 gaatctggag ttcatccaac caagcatccg cttcaggctg cccggccatt gaacagcaca
     7621 gaaaaagcgt attgggaaat tggcgtaata tcaaggggat actttgaaaa aaagtgtcat
     7681 ttcggacgct tcccgggcga aaaccacgcg ggttaatcac aatattccag ttttcgggca
     7741 accaattcag tcccacttgt tccttttcag ccgttattcg cactttggta aaatcaattc
     7801 cgccgttacc ggggacgact aaagccggct catcggttgg aaagccccaa tttttcccca
     7861 gtcgaatatc gcggtttgta tccgccgcca gtcctctgac tttttttagc gttttttggg
     7921 tcaattgacg catcattgcg gaaccctgag catgtagtgt gatatcgttt ccccaaatcg
     7981 aaaccgcaca cggaatattt ccccgataaa aagaaagcag catgccttca taagggattc
     8041 gtaacgcatg aatcaaatcg ggatttgttt ggcttacgat cctgttgaat ttccgggcgg
     8101 taagcggtaa cgaaaaaggt cccaagatat acctgaaatt gatcagcaac ggacggaatc
     8161 ttctgataaa agtccggtcg gacccttcgg atttactccc atcttttgct ttgtttttgg
     8221 gaatcgatga accggataag cccgagaaag cgacgggaag aataaaaaat tcatccgcct
     8281 cttgcggctg ttcgcaagga taacttgaaa ccagaacaat ctgatgattt aagcttttca
     8341 gacttcgcaa ccaattcagc gtaatcgggc ttcttccgtc cgcaaggatc aaaatcttca
     8401 taatttcctt ttttctgtga ctgaaataaa atctacttcg aaaatatttt attctttccg
     8461 atttcgaaca gcgaacgaga ttgcctgttc aaatgattcc gccatatttt ctaaattaat
     8521 ttcattccgc acgatctgaa aagatcggtc acccattttt cgcaaccgcg acggatcgct
     8581 cagcaaagtc cggatggcat ctgtcaacat ttttaaatca ccgggcttga ccaagatgcc
     8641 gttttcttcc cgtaccaaat ccgtctgtgt accgtccgct tcggcggcga ttaccggcaa
     8701 gcctgacgcc attgcctgtt gaagtgccaa tccccctgtc ccgggtaaga cgaataaatc
     8761 agcctgatca aatttttcgg tcaattcctg cccataaaga gctccgaaaa acaatgtttg
     8821 cggaaaaatc ttcgcagcta attccgtaat ctgttgtctg gccggtccgt cgccgacaat
     8881 ccaaaggatc gggctcacgg aatctttcaa agcggcacaa gcctcgatga gcaaatcaac
     8941 ccgcttcctt tcctgcaatc ttccgacgaa aagaatcacg ggatgatcgg atatttccgg
     9001 actccgttcg ggtggcaaat cccttttcgg tcgttcggta acggcatttt ttgcgataaa
     9061 gatcttttca ggagaaacac ccatttttcg ataagattcc gccccggctc cgctgtaagt
     9121 caaaaccgca tcaaattgac ggaaaaatct tcttttcaaa cggttttggc taaaacccgc
     9181 tcccaacccc catccgatta ccggcttgtt ttgccgatgc atccaatgaa ttgcccatcg
     9241 ggttaaagga tagcgattat tcgcctctac gatcaagaca tccggatttt ggcttctcaa
     9301 ccattgaaag atattcgttt gaaagcaaag gtaaaatttg ccatgaaata ggtgcttgtt
     9361 atttgcatgg taaaaatggg ctttttccaa tttccgcgaa gtatcgatca tttcatcttt
     9421 ccgcggatca ccggcaaata ccgacacatt cccgtcgtat gattccgcca aaagatcaaa
     9481 caatggaacg cgatactctg ccagaactcg ttggacaatg ccgattttca ttcgatcaca
     9541 tttacccgcg atccgtcagg tgttttttca acttcgattc gagacggaaa ggcatctttg
     9601 agttcatcca aatgggtaat taccagaatt tttttataat catcctgaac ggcgattatc
     9661 gcttcgatca atctctggcg cccttcttca tcctgactgc cgaagccttc atcaataacc
     9721 agcatctgta accgggcacc ggctcttttg gtaagaatat gggaaagcgc caaacgaatc
     9781 gcaaaattga tccgaaacgc ctcccctccg gaaaataatt cgtattctcg cgatcctgtc
     9841 tcatcgctga taaaaatagc caaggtctct attttatctt cccggttctt atctttgtac
     9901 tctttttgag tggaaaagcg gacagacatt ctttcatccg tcaattttcc cagaatcaga
     9961 ttggcatgct cttcgatttc aggaagtgcc tgatcaatta agattgccgg gattccgttt
    10021 tttgaaaaag ctttttcaag gtatttcagc tgagaaattt gaaggttggc ttcctcccgt
    10081 tcgcgaatga cctcctgttt ctgctttttc aatgactcta agacggaaac gagctgttcc
    10141 gcagctcctt tttccaaccg gatgcggttt gtttcagctt gaacggtatc aaattccttc
    10201 ttcgcctttt gtaaatccgg taagtttgcc gaaaattcgt ccagcgccgc tttttgctgt
    10261 tccacggagc cttcaatctg ctttaattct ttttcatcgg cagcgatctg attggttaaa
    10321 tcgataattt cacgttgaat cggctgtaaa gcggcttttg cctgctgtac ttcctgccag
    10381 cggttttcgt tcagagccaa ttcggattct ttttcccgac attgctcatg ggccgcggga
    10441 ttatatccga gagcttttgc ctgttcgtcg agcacagata acctttgccg ctcttccgcg
    10501 cagaaagatt ccgtttccaa caatttttga atttcgttca agcgatcgga taatttttgg
    10561 cgataaggtt cattttcctg aatttttttg atctcggcgg aaatcggtgc gagtaaacgt
    10621 gaaagctcga gatgcttttt ttcagcattt tggatttccg ataatttccg attaagggca
    10681 tctttttcct catttagctt tttcagttct tcgctattaa ttcggtatcg atctcccaac
    10741 tttttcccgt caagctgcat gctttgtaaa taggtgagag tatgttcctc atccaattcc
    10801 cttccgcaaa aagggcaagg ggtccccact tgagctgcct gaagttccgt tattcgcttc
    10861 tttaaatccc gcatttgggc atccagttgt tgattctccg cccgtttttc tacgatgatt
    10921 tccgtaatgt tccgcagaga ttcattcaca gattcttttt cggaaaggaa tgcttcaata
    10981 ctttcaagtt gtgtttgaag ctctttttgt ttttgggata attccggtat taattcgaaa
    11041 tttttttcca gcctgagaat ttcttgtcga agtgtggctt gttcggattc caaaccggca
    11101 cgcaattgac cgattttacc tgttatctct tttcgctctc cgtcgatccg ataattttcc
    11161 agcgcttttt tgttccattc ggataattct tcccgaattt tctgtaattg ccggtatccc
    11221 gactcgattt ccggctcctc tttcagcaat tcctgaatag acaaagattc gagagttcgt
    11281 ttcgacaacc gacttttttg tgcatcgatt tgcctggcac tcttttcgta attctgcaga
    11341 agagactgat aagcggcttg atgggattcc aaaagcgttt cggcggattt tgcattttgc
    11401 caggcttgtt gtttgatcac caactgctct tcggactttt tcaattcttc gtccaagctg
    11461 ttcaaaactt ctctgcgccg gggttcttcg gataattctt tttcgatttc atccagtcga
    11521 ttctcaagaa aagataatct cacagtctga tttcggcgtt tttcggctgc ggcggtttgg
    11581 tatttttccc aaatatccaa ttctaagatg ttggaaagga ttttttttcg gtcaccggcg
    11641 ctttgacttg taaattgatc cgcttttcct tgcagaaaaa aagaagcatt gatgaatgtc
    11701 tcaaaatcga ggtgcagcag ctcttcgatt cttttatttg tctgacgaac ggatttttcg
    11761 gtcatcggat gccagtttcc cgtttcctga ttccaaatca acaaatcgac agtggaagtt
    11821 tttccgcgtc tgagacagcg aatgattcga taaatatttg attcatattc gaactcaaat
    11881 gtcacttccg ccgcatcgat atttctggaa ttattaataa tcgtctcgtc atttttccga
    11941 gcttggccga acaaagccca agtcatcgca tccaaaaggg cggattttcc ggcgccgttc
    12001 gagccggaaa tacaggcaac atggatccgg gtaaagtcaa tctccaccgg ttctttatag
    12061 gaaagaaaac cgctgatttt cagcagacgt ggaatcattt tttatccttt gggaaaattt
    12121 cacatttcat gatttgactt attcttccga atcggtctta tcggagtcat cttcagatgg
    12181 ggaagctaat ttggtaacca ttactttatc gattcgacga ttatccatat ccacaatttc
    12241 aaatcgatag ccgtccgctt cgaaagaatc cccggctttg gggataaagc cgaatttgct
    12301 taacataaac ccggccagcg tctgataacc gactcgatct tcgtcagcga gttgtttaat
    12361 tccgagttca tctttaaact cttcaatatc catttgaccg tcaatcagcc atgaaccgtc
    12421 ttccctttcg acaatttcgg gatgataggt atcatataaa ccgggaacgt ctccgaccag
    12481 ttcttccaaa atatccaaca aggtcaccat gccggaaaat ccgccgtact catccaaaac
    12541 aatcgccacc tgcttgccgg attccctcaa atcatccacg acggacagtg ccggtttgct
    12601 ttcgggaata aacagagggg tttctgccat tttgctgaga tcaatttcat caccgctgat
    12661 atatgcatcc aaaatttttt tcgccgagac aattccgacc acgttatcgg ttgttccccg
    12721 aaccaaagga aaataactgt catccgattc cttaatttcc gccaggcttt cttccaatgg
    12781 ttcgtccaaa tcgatccagc ataattcggt tcggggggtc atcatcccat caaccgtttt
    12841 atcactcatt cggaatacac tctcgacaat atcctgttcg gtttcatcaa agacaccgga
    12901 gagagttccc tgctcgatca ttactttgac ttcatcttcc gaaacagaat cgtcttcggt
    12961 tttcttgaca cccaaaattt tgataccgat ttcggttgac caacttaaga agctgaccag
    13021 cggcttggtg attcgggaca gcacacgcat tatcccgctc atgttcagag agatcttttc
    13081 aggaaaagaa attgcgagcc gtttcggaat caattcaccg attaccaaac tgaaataagt
    13141 gatcacgatc gaaacaagca aaagcgctaa ggtatttgct atggaggaga aaaggggaac
    13201 ctttgccagt aaattggcta cgggaccgga aaaggcggag gcgccgaccg caccggtgat
    13261 aacaccgacc aatgtgatga aaatctgaat ggttgagaaa aagtcattcg gattttcata
    13321 caactccaag gctgtccttg cttttcggtt tcctttctcc atttcatgct ggaggcgggc
    13381 ttttcgaacc gaaacaatcg ccatttccgt cattgaaaaa attccgttaa taataatcag
    13441 aacaactaat aataaaaaac tgctaagtgg ttgtgacata acctttcaaa tccatcattt
    13501 taaaaaaacc ggtaccctat tttcaggttt cagaaccttc agaaatcccc atatccacat
    13561 cattaaatat ttgctttgcg agattcagca atccgggaag cgcatttttg tcatatttat
    13621 ttgattcaca ataacgttcc agcagctcta aatggctcag gctgcttaca gcggcatctt
    13681 ccgaaaggcg gatccgcgga gatgaaatgg gcctcaatac caaatgaaaa gccagtgcgc
    13741 tttctgcggc ttttcgtaat tctctttcat ccaaaaaatt ctcccattcc cgcgggtagt
    13801 gaatgattaa acggatcatc gcatccggaa tttgatcagc ctccggcaaa gcggccaaag
    13861 cttcctgttg aaaagtctcg gcattttgaa tagtgatctc gttggagaaa aaattcctgc
    13921 cttctaactc acgaaaagta taagaggaat gccggttatc gatttgagcg atgacaaacc
    13981 cttttttatc attgcattcc ccgaaattaa ctctttcgat tgaaccgggg taaaccacag
    14041 ggggttgatt cccggcattc aagtcttgaa atttatgaat atgtcctaaa gcagtatagg
    14101 atatccgccg atctttcatc actccctcgg ggagtagcac ctcctgcccc aaagcagcca
    14161 tttgaaattc ggagaacaga gcaccggtta tggaataatg tgccatcaga ataatcggga
    14221 gggatggatc cgtttcatca agaatacgat tgatttccgt cgatatttga tatgcgagat
    14281 cctgtccggt actttcggtt tctttgccaa ttgactgggt tgccgccacc agacgggagc
    14341 gaggaatcca aggaacagcg attacctgaa cgggaacacc atccaacatt tccggcgtcc
    14401 aaaggctgat tgttttggca aggtgcagat aaggaacgct caaagtgtca tattcctgta
    14461 aagcgctggc acggccgctt gccgtagaag agtcatgatt gccggcgatg agcagtaccg
    14521 gaattttcgc ctccgacagg cgcatcatcc tttttcccca ttcgcgctga aaagtcggaa
    14581 caggaacagc gtctcgatag gcatccccgg caaacaaaac aaggtccact ttttcatcga
    14641 tcgcgctatc tacaattcgg tctaatgcct tgagaaaatc aagcgtacgg taagagaatc
    14701 cgcttttcgg atcgcgcttt ccaccggtga taatatcgat atgagcatcc gcaaaatgca
    14761 aaactttaat actcatgatc ctcccatccc gatttccgcg gataaggaac tgcatgccac
    14821 gaagccgggg tgaatatcca gacaggattg aacatcagcc tgagcggatt caaattctcc
    14881 gagcgctgct tccgccatcg cccgccaata atatgactct tccaaataag gttcattggt
    14941 actggctaaa gtgatatttg ccaaattgat cacatcctga taacgtcccg aataataata
    15001 ggcataatac ggaccggtct gataccagac gatacgatac ggccttgttt ttgtatccaa
    15061 atttgcatag tatgaaaatg ccatgtcata agattgtgcc gctccgtaat aatccgttaa
    15121 ggcgacttgt gaagtaccgc gattaaaata agcataaaat cgcgattcat catcctgtgc
    15181 ctccgctact tgattgtttg ccatttcaaa agcattctgt ttcgcccaat cactgttgac
    15241 atacggtccg agcaaactat agagttcggc ttctttatct ccgggataga cgatcataaa
    15301 ttcaaagtta aaacatttcc attctttata gagcgtttca taagtaactt cataattcgg
    15361 tttgtaatat gaatcctgaa ccgtccaaat ctgtgtagca tcattataac cagtaataac
    15421 gttatagtgt cccatccaac tgtaggcacc gctgacttcc tgcatgaaag accctttttc
    15481 gatcaaaatc ggaaagccgg ctgcaatcaa ttttttcaaa agttccggtg ttcctcccga
    15541 tctgacaatc gatcgcaact gtgtttgttc tgcgacaaag ttttccattt cataaaacat
    15601 gacattttta tctttgtcaa aaggtttcaa ccactttccc gcatccaatc gatttccttt
    15661 ccacccccaa aatgtgagcg ccattgataa attggtgggg gcgcagtaat tccaaatccc
    15721 atgttggtct tcgtacttga caccgtcgat gtgagcactt tcgggcaaag gggtcggagt
    15781 gatcgttgcg gtcggtttca gagtcggcgt tgccgtcgca gccggcgtga cactgacagc
    15841 cgtttcggtg ggagggacgg tcattgtcat tgttattgtc ggggtgtgag ttccttcggc
    15901 agtctgagat aacatcgaaa tgttttcttc gcctccctga gggacaaaca cggtatcggc
    15961 aggtgaattg gaaagcatat agagtctgtt cttcaggttt tcgactcgaa aactgatctt
    16021 gtcgcgaacg ggagtaaata acccgacaaa aagtatgaaa aataaaatga ttccaaacag
    16081 aataataggg cgataatccc tgtcattttt cataaaaata ttctatcaaa gagaaatttt
    16141 atatggatat tgcattcaaa aagggaaatt ccgaatggag cgaatgattc caaaataatt
    16201 aatcttcatc ttcatccaaa tgaacttcca atttaatttg ttcgggctta atccttaacc
    16261 aaaggacaag tttttgccct tcttccgtat ccagacagcg ggcggctaca atcggcactt
    16321 cctgttcgag cccgattgag tttcgaaatt gcaaatacat ggtatcctgt tgctcccatg
    16381 tcgattgaca gcgttcaatc aatccgggac cgttcaaggt gcgtaaattt gtcatcacca
    16441 cttcataatt ggccggagat tcaacgatcg cagccgtcca atcggaattg gtcgcttcaa
    16501 aaatcggagt gggtccttct ataatcgtaa tcttgttatt cataataaaa ccttctgttt
    16561 atcataccat aatgtcatct tttatttctt aaaaccttat taaatggttt ttaaatagtc
    16621 aagactaaaa ggaagaacct ccattagagc cgaagttgtt atcctgatga ttttccgcaa
    16681 aacttgattg ctcatccaat agtggcgtcg atattaggac atttgtaaat agccttgaca
    16741 caccattatg gttgaataa
//
LOCUS       DAOSMM010000051        16099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3657_length_16099_cov_17.163176, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000051 DAOSMM010000000
VERSION     DAOSMM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 16099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16099
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3657_length_16099_cov_17.163176"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PK898_07725"
     CDS             complement(<1..778)
                     /locus_tag="PK898_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU19958.1"
                     /translation="MQYRKFGKTGETVSVLGFGCMRLPVLDGKPEKIDVAKTDEMLNY
                     AFDHGVNYFDTAYTYHRPMNPAVKKGFSEDVLGDCFRGAKREKIFFATKLPTFNIYKK
                     EEFEEALNASLRSLKTDRIDFYLAHSLTKPTWDRMVELGMLDFLQKAKESGKVRHLGF
                     SFHDDLSVFKEIINTFDWEFAQIQYNYLDVNRQAGTEGLHYAFSKGLGMTIMEPLRGG
                     KLVSIPSREISDLWKESEHRDWTPAQRGLYFVWNDPAVSVV"
     gene            942..1859
                     /locus_tag="PK898_07730"
     CDS             942..1859
                     /locus_tag="PK898_07730"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOU19959.1"
                     /translation="MKTIFRFLFIFALLISIGSCGTINQMIHPSTATPTATLTATITP
                     TPLPTLTPTPPPAVAIVNGIYIWQEDFDMTVANLRRAYSDTQSMQPSEETLKQEALDT
                     LIDETLFQAAAAKDGIIISDEELDTRIQSLIEKAGGSDAFRQWLSENHYSESGFRRAL
                     TREISAAKIREKLFSEKLTGIEQIHAYQIFTATHAEANTIKTKLDLGLNFIDLAKTND
                     PITGGDLDWVARGILVYPQLEDALFALQPGSYSDIIETDIGFHILYAAERSTDHALTP
                     QSRQILEHKILSDWVKEQKSSAEIIMLNK"
     gene            1871..2740
                     /locus_tag="PK898_07735"
     CDS             1871..2740
                     /locus_tag="PK898_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19960.1"
                     /translation="MPTTRYDPDLLDRIDRKRKKKRRESCLSRLLLMILILSIIIAVF
                     FVWVLTQKNKKKPIETPGIETSWYEQTFDVLFSSITPTQTDTFTPTLPTPTVTQTNTM
                     TPTVSLTPTQTPTPRLRTRTPSGGNQLPTSTPLLLLNAAETEIANLKIAETSISASST
                     PAATPDMWFEIQGQPGATNAEIIYPNAECSWMGVAGLVNDARGDPIIGLYVQVGNFPD
                     GEIRETLTGLFPIYGESGYEITIARPVMEIPQPLWIQLLNESHVPVSEKVYFKPSNSC
                     ERSLILINFKRVR"
     gene            2913..3926
                     /locus_tag="PK898_07740"
     CDS             2913..3926
                     /locus_tag="PK898_07740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOU19961.1"
                     /translation="MKNKAKYLFQSSFLFFCLILLISGCNLKEKRDQKLATEIAVIST
                     EIQSTLQAAQTQTVASIPTDTPLPTFTPTPPATETPTPTVEFLPTATATPDVRPSADE
                     WANWPILPTLSENAKKIFWHGVNDLKTNPKVFSKIGDCQSRPNVFLGIYDMGYEGILA
                     DEDLYLQAAIDYYKGSFLMESYAVHDGMNVGSVLTTTWADPKACHKDENALECEIRIH
                     NPSIMFVNLGTNWVSGVSTDAYYDYLAEIVQVLIDHGILPVLSSKADNVEGDNSINRI
                     TAQVARDFDVPFFNFWLAAQSLENHGLSKEKPIYLSVSAWDYRNYYALKLLYNFGNEL
                     GLF"
     gene            4257..4328
                     /locus_tag="PK898_07745"
     tRNA            4257..4328
                     /locus_tag="PK898_07745"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4289..4291,aa:Lys,seq:ttt)
     gene            4413..4883
                     /gene="greA"
                     /locus_tag="PK898_07750"
     CDS             4413..4883
                     /gene="greA"
                     /locus_tag="PK898_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU19962.1"
                     /translation="MTNTMLTREGYDKLVAELDYLRNVKRAEVSERLRNAIDGGGDDL
                     LENAEYEAAKNEQSFIEGRISDLEYLIAVAKIVDSPEKIGVADIGSKIKIQEEGSDEI
                     ETYTIVGAPEADPKENKISNESPIGKAVLGHHKGDIVQVSAPVGSYNVKIIDIE"
     gene            4919..6445
                     /gene="lysS"
                     /locus_tag="PK898_07755"
     CDS             4919..6445
                     /gene="lysS"
                     /locus_tag="PK898_07755"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOU19963.1"
                     /translation="MDFNELEKVRVQKIQKLKELGIPAYPTRSYISSTIKEACEAFEK
                     AESTGSDQPIEVTIAGRMRSLRIMGKLAFTHIEDGTGRIQLFLRVNELGDQLQFFKDY
                     FDIGDFVEAHGTVIRTRSGEISLQTTSIRMLAKAISPLPAAKEEVVDGVTVKHTGLSD
                     PEIRYRQRYADLVVNPEVRNIFRTRSKIVSALREFLDSHDFLEVETPVLQPIYGGAAA
                     RPFITHHNQLHQDLYLRISFELYLKRLLVGGLDRVYEIGRDFRNEGVSYKHNPEFTQL
                     EFYCAYFDYLKVMDFTEQMIRYAAEKALGTTKLKYGDVEFDLAKPWNRVNMREGILEK
                     TGIDFEKYPTTESLAAAMNEKGIQTKPGLQRGKLINQLVSDFLEPAFLQPTFLYDYPW
                     EVSPLAKRKPENPLITERFEGFIAGMELCNAFTELNDPIDQEARFLEMGRTYAEDDEE
                     RNPIDEDYLRAMRYGMPPCGGFGMGIDRLTMLLTNQSTIREVILFPHLRERDKDQEDN
                     "
     gene            6445..7122
                     /locus_tag="PK898_07760"
     CDS             6445..7122
                     /locus_tag="PK898_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU19964.1"
                     /translation="MNFAVLWDLDGTISDSFQCFYMALERILSEQKPTIKITPEEYKS
                     QYFGATFPSILDRMYQGRLSDTQIWELNERYFKYSVEFARQGTVFPIPGVESFLKKLH
                     AADIRMAIASSSTVEMIFTELDVLGFISYFDNIISGNLLPSKPAPDVFQIAAASIRAQ
                     PSQCVVIEDSVAGITAAKNAGMRTIAVTTTNPADKFGQADRVTEDFRKFDLDALKSLF
                     VTDFSNL"
     gene            7492..7671
                     /locus_tag="PK898_07765"
     CDS             7492..7671
                     /locus_tag="PK898_07765"
                     /inference="COORDINATES: protein motif:HMM:NF039193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HOU19965.1"
                     /translation="MEIEGYCVKCKAKRTIQKPTAGFNKRGSAVVYGTCPVCGTKIYR
                     IGRTELHKGMEAPKK"
     gene            7751..8536
                     /locus_tag="PK898_07770"
     CDS             7751..8536
                     /locus_tag="PK898_07770"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU19966.1"
                     /translation="MDTLELESFLSDLPMGGIKYFEIVDSTNRYALEWAEKGAEDFSL
                     VVANEQTAGRGRMARKWLTTPGNSLAMSLILRPSHKEQKQIHLFSPLAGLAVAESLKN
                     TYGLNPLIKWPNDILLDHKKVCGILCEAAWDKDKLRGMVIGIGLNLKKESIPTDDSLR
                     YPASSLESAGCDCLEVLPHIRNILLEFRRLRPILATSEFITKWESYLAFKGEQVILTA
                     PEKDPEIFTLIGLDAEGNLHVIDSAGDEKFFQAGEISLRPFKS"
     gene            complement(8607..10043)
                     /gene="gatB"
                     /locus_tag="PK898_07775"
     CDS             complement(8607..10043)
                     /gene="gatB"
                     /locus_tag="PK898_07775"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOU19967.1"
                     /translation="MTEYEVTIGLETHIQLNTVTKAFCSCKADSWGEEPNTNICPVCT
                     GQPGTLPRTNKAMVEKALMLCLAMNADINRKSFFDRKNYLYADLPAGYQVTQNDMPIG
                     KGGYLDFFMDDGRKIRVHIDNLHMEEDAGKTKNEGDIRLIDMNRCGVPLVEMVTKPDL
                     HSADEAATYLMRLRQLLRWIGVSEANMEKAHLRCDANVSIRPKGAKELGKKCEIKNIN
                     SIEAVRAAINSEVERQTRELNAGNKVEQWTIEWDNDAQELRKMRSKETAADYRYFREP
                     NLMPVVITEEFLEEVKAKMPELPIQRRERFINELGLPEYDASILTGERDTSEFFEAAC
                     KIYGKDNKRVSNWMMNEVLRVVNEKGISVSELRITPQDLADIISLVDSKRINTSTGKT
                     LVDKVEETGKSPAEIVEESGLGLVSDDSEIRKVCERIITDNPKEVGLYKGGKETLIGW
                     FVGQVMREMRGKADPVLTKSILEELLKQ"
     gene            complement(10058..11647)
                     /locus_tag="PK898_07780"
     CDS             complement(10058..11647)
                     /locus_tag="PK898_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOU19968.1"
                     /translation="MTELTSMSALDQARLIREKKISAEELLNETLAQVKKVDGRPGSL
                     EEYIEQPEDKEKVHAFILITEESAREKARAVDRKVAAGEELGPLAGVPYSAKDVYCTK
                     GIQTTAASRILDGYHPPYTSTVIQRLERADAVLFGKANLDEFTFGGSNETTAFKPTVR
                     NPWALDHVPGGSSGGSAAAVAANEGAISVGTDTGGSIREPAAFTGTVGLKPTYGRVSR
                     YGVIPFASSMDSMGPITRTVADAAAVLREIAGFDPNDSVTGRVPVDNYPEELKKGVKG
                     MRIGVSPDYMRITYLDENGNYSSTAIDPEIESCVRKALRIYKDLGAEIIEDVPMTNTK
                     YSVPAYFVITRIEAYSNLQRYDGLKYGHTTKRPVEDMYDLYYKSRGEGFGYQAKLRLL
                     TGLFASQEKFYEKYYLRAQRARAMLRNDFDEAFDPNGKYRLDVLLTPSTPTPAFQFGG
                     VTATDPLLMQFADQFTSPMNFAGTPGISFPCGLSSEGLPIGLQAAGYDYCESKILRAA
                     YAFEQATSDEDWRKVKPVVLR"
     gene            complement(11651..11974)
                     /locus_tag="PK898_07785"
     CDS             complement(11651..11974)
                     /locus_tag="PK898_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19969.1"
                     /translation="MDNDAITKEMLDDFVHVAEVELSTEEFESILAKMNDQLTVIHEL
                     TAIPLEGNLPPVIHGNPFPEEIQAPLREDVWIPFEHPEDIISQAPRTQDGYIVSPDVP
                     HQTLE"
     gene            12336..14315
                     /locus_tag="PK898_07790"
     CDS             12336..14315
                     /locus_tag="PK898_07790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU19970.1"
                     /translation="MTFLNRVGKLNLFFTQKDFKNIQYDAGYAWIADINDPKIAIGYN
                     LPVTLYENDLKILRDPKATTESVREQGGGKFVIWDNGTICFSTSDNSNPTQNGRTYRV
                     EYPRIVEKRTVRIIFAFTFLLGFLWIGFSLPGKRKKFDLRSTILGKNRKLFLFSLLAL
                     IPFLYFAIKFLLPQPLWGDELFTLKNYVYAKDWYYPATFYDYPNNHVFFSLIISLYSK
                     FIKIRTFCDAVQSPWKIRLLLIFLSIITIFFTTLSARKINKSAGVIASILLSTSLPFF
                     LWTTQIRGYGLSMTLMSILIYLLMRVQTSPNRISFVSIAIVSALFVYIMPTNAVYIIA
                     MGFYFLFKLLIDLGKYKESGENGIIYRIKRNPEAKILYSFLWGAVFSIMLYYPILDQM
                     MHVYLPESGEKVIMSRELFFRIFRLGTVEIIRSSLVGQPLLSACILIGFFLFLRNPGE
                     NKSLSRMVFGTGLCLLIIPDLFFFLLGSVPYARNSLAILPVIILIFASLIAPIPDALS
                     DPRLKNISFLVLLISSGAFFSDHLVRLSKEPLSYGNNSLNNLTEPFFLGDHLNFNEVL
                     TELKAKNAAGLPVLLKSPAPYFIESICDCYQRVCYGDGSGEGQTLLKNHAPYLLIQTD
                     WKEFSANSEKYPDLSRCTTINQENRTSFKVFLCNP"
     gene            14329..15351
                     /locus_tag="PK898_07795"
     CDS             14329..15351
                     /locus_tag="PK898_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOU19971.1"
                     /translation="MTKNKILFPLAILICLALFAVPIKVFSEESIEVVFNDPFGKNHD
                     PAIQDLILNAFENAENHIYIAIYNFTDTACRDALIDAVKRGVEVRLVIYSENADNRVI
                     GDLTKNGIKIVKSQSKGLMHAKFIVVDYETTISGSANLTPGSFFYDNNFMILIHDSKV
                     NKIFQDEFNEMFIEKKFGPTGQPTIPPTAVSLKDGTKITVKFSPEDGVEKILLSLIRA
                     SQKSIDVLAYSFTSDNLGNALIDRYDDGVNVEVLFEKETSNQYGSEAAYLVRNGIPTY
                     MDGLEDGLMHEKVMIFDESVVAAGSYNFTRAAETVNDEQILIIDNRKIADQFEEEFEK
                     ILEKAN"
     gene            complement(15513..>16099)
                     /locus_tag="PK898_07800"
     CDS             complement(15513..>16099)
                     /locus_tag="PK898_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU19972.1"
                     /translation="YLKDEKVYAPNGDHVTMEEIALHSLHRKDQRQIAAYASHVSPEC
                     PPVFGTEFAEVEVDIETGQVTPIKLVMTVSPGTVINPTTAIGQLEGGQTTALGYALCE
                     EMCYDNQGNLLNPRFGDYHILQADEMPLMESYIIQEGDGEKTGPFGAKALAEIPTDGV
                     APAIANAVANAIGSRIDSLPIYPEKVWRAIKSQH"
BASE COUNT         4499 a         3622 c         3367 g         4611 t
ORIGIN      
        1 aaaccaccga gactgccgga tcattccata caaaatacaa acctcgctgt gccggtgtcc
       61 aatcccgatg ttcggattcc ttccaaaggt cactgatttc tcttgaggga atggatacca
      121 actttccgcc ccgtaaaggt tccataatcg tcattcccaa tcctttggaa aatgcgtaat
      181 gcaatccttc ggtaccggct tgcctgttga cgtctaaata gttatattga atttgagcga
      241 actcccaatc aaaagtatta ataatttcct taaatacgga taaatcatca tgaaacgaga
      301 aaccgaggtg gcggactttt ccggattctt tcgccttttg taaaaaatcc aacatcccca
      361 attcaaccat gcgatcccaa gtcggcttcg ttaaactatg cgccaggtaa aaatcgattc
      421 gatctgtttt caaggaacgc agagaggcat tcaaagcttc ttcaaattct tcttttttat
      481 agatattaaa ggtcggcaat ttagtggcaa agaatatttt ttcccgcttt gctcctcgaa
      541 aacaatcacc gagcacatct tcgctaaacc cttttttgac tgccggattc atcggacgat
      601 gataggtata agcggtatca aaataattta caccgtgatc aaaagcataa ttcagcattt
      661 catcggtttt cgccacatca atcttttcgg gtttcccatc caacaccggc aaacgcatac
      721 aaccgaaccc cagtaccgaa acggtctcac ccgttttccc aaattttctg tattgcattt
      781 ttcgacctcc cccggaaata aactccggat caaatgttat cgataaagat taagaatatt
      841 gtatttcaat cttccggatt tcggaaataa aaaaatgcac tcatcggaat atttttcttt
      901 tcatcctcta tatttcaaac acttagtata ataatttcct gatgaagacc atattccgat
      961 ttttatttat tttcgccctg ctgatttcga tcggttcgtg cggcacaatc aatcaaatga
     1021 ttcatccgtc tacagccacg ccgacagcga cattgaccgc caccattact ccgaccccct
     1081 taccaaccct cacaccgaca ccgccgccgg cagtggcaat tgtaaacggt atttatattt
     1141 ggcaggaaga ttttgatatg accgttgcca accttcgccg ggcatattca gatacacaat
     1201 cgatgcaacc ttccgaagaa actttgaaac aagaagcgtt ggatacctta atcgatgaaa
     1261 ccttattcca ggctgccgcg gcaaaagacg ggatcatcat ttcggatgaa gaattggata
     1321 cccgaattca atctctcatt gaaaaggcgg gaggatcgga tgcttttcga caatggttat
     1381 ccgagaatca ttacagtgaa agcggctttc ggcgagcatt aacccgtgaa atttctgccg
     1441 ccaaaattcg ggaaaaactc ttttcggaaa aattaacggg aatcgaacaa attcatgcct
     1501 atcagatctt taccgctacc catgccgaag ccaataccat taaaacaaaa ctggatttgg
     1561 ggctgaattt tatcgatctt gccaaaacca acgatcccat taccggcggt gatttggatt
     1621 gggtcgcacg aggcattttg gtttatccgc aattggaaga tgcgcttttt gccctgcaac
     1681 cgggaagcta cagcgatatc atcgagacgg atatcggatt ccacatcttg tatgccgcgg
     1741 agagatcaac ggatcatgca ctgacaccgc agtcccgcca aattctcgaa cacaagatcc
     1801 tatcggattg ggtcaaggaa caaaaatctt ccgcggaaat catcatgctg aataaataaa
     1861 gattcttaaa atgccgacaa cacgatacga tccggatctc cttgaccgga tagacagaaa
     1921 aaggaagaaa aaacggagag aatcttgtct gtctcgcctg cttctgatga tattgatcct
     1981 ttcgatcatt atcgccgttt tctttgtttg ggttctcaca caaaaaaata aaaaaaagcc
     2041 catcgaaaca ccgggcattg aaaccagttg gtatgaacag acttttgatg ttcttttttc
     2101 gtcgattaca ccaactcaaa ccgacacgtt tactccgacc cttccgactc cgaccgtcac
     2161 tcagacaaat acaatgactc cgaccgtttc tcttactccg actcaaactc cgacgccgag
     2221 attaagaacc cgaacgccgt ccggcggaaa tcagctccct accagcacac ctcttctgtt
     2281 attaaatgcc gcggaaacgg aaattgccaa tttaaaaatc gccgagactt cgatcagcgc
     2341 ttcgtctacg cccgctgcca cccctgatat gtggtttgaa attcaaggtc aacccggtgc
     2401 gacgaacgcg gagatcatct atcccaatgc cgaatgctct tggatggggg ttgccggatt
     2461 ggtgaatgat gccagagggg atcccatcat cggattgtat gtccaagtcg ggaacttccc
     2521 agacggtgaa atccgagaaa ctctaaccgg tcttttccct atctatgggg aatccggtta
     2581 tgaaattacc atagcccgac cggttatgga aattccgcag cctctttgga ttcaattatt
     2641 gaatgaatcc catgtgccgg tttcggaaaa agtttatttc aaaccgagca acagttgcga
     2701 gcgcagtttg atactgatca acttcaaacg ggtacgttag accgaagaaa accgaaaaat
     2761 cacattggat catctatctt ctctttaaca agtcggaatc gatgatcaac attaccaaag
     2821 agatccttta taaagattta ttcacaatcc gtcggagaag tatgaaccgc ccttgctttg
     2881 ttttttaatt ttcattgtaa aatacttaag gtatgaaaaa taaagcaaaa tatttgtttc
     2941 aatcgtcttt tctttttttc tgccttattt tattgatttc cggctgcaat ctgaaagaaa
     3001 aacgagatca aaagttggca accgaaatcg cggttatttc caccgaaatt cagagcacat
     3061 tgcaagcggc acagacccaa accgtcgcaa gtattccgac cgatactccg cttccgacct
     3121 tcactccgac tccacccgct acggaaacac cgaccccgac cgtcgaattt ttgccgacgg
     3181 caactgccac tccggatgta cgaccttccg cggatgaatg ggcaaattgg ccgattcttc
     3241 ccactttatc cgaaaatgcg aaaaagattt tctggcacgg tgtgaacgat ctgaaaacca
     3301 accccaaagt tttttccaaa atcggggatt gccaatcgcg tccgaacgtt tttctgggga
     3361 tatatgatat ggggtatgaa gggattttag cggatgaaga tctttatctt caggcagcca
     3421 ttgattatta taaaggatcg ttcttgatgg aaagctatgc cgttcatgac gggatgaatg
     3481 tcggatcggt tttaaccacc acctgggctg accccaaagc ttgtcataaa gatgaaaatg
     3541 cactggaatg cgaaattcgt attcataatc cgtccattat gtttgttaat ctaggtacga
     3601 attgggtcag cggagtcagt acagacgcct actacgatta cctggctgaa attgtgcagg
     3661 ttttgatcga tcatgggatt ctgcctgttt tgtccagcaa ggcagataac gtggagggag
     3721 acaacagtat taaccggatt accgcgcaag tggccagaga ttttgatgtg ccctttttca
     3781 atttttggct tgcagcgcaa agtttggaaa accatggact ttccaaggag aaacccattt
     3841 atttatctgt cagtgcttgg gactatcgga attactacgc cttgaaattg ctctataact
     3901 tcggaaacga actgggactc ttttaattca tcggatcagt tgattaagta aaaaacctct
     3961 gaaagatcag aggttttatt atgaaaattt ttgccttctt ttctttttcc agaaggcagg
     4021 aatgactcga gcttttttcc ccctactagc tacaaagtgc tgttttccaa taattcacag
     4081 cctttattat agttttttga acaggcttgt caagaggtta taatattaaa gttatattaa
     4141 tcttttgaat ataaaaggat gcattttaaa cgaatcgtta ttcgaaatga tacttatttt
     4201 caccggctta ttgacacaag ataatcggca tgataaaatg tacgagttac agtcaagggc
     4261 gtatagctca atggtagagc agtggccttt taagccattt gttctgggtt cgagccccag
     4321 tacgctcact cggccggaaa ttgctctcct cactcgagga gggtattttt gttaaatttt
     4381 aaatttttat attcttttag atcgaggtca ttatgacaaa cacaatgcta actcgcgaag
     4441 gctatgataa actggtcgcg gaattggatt atctgcgaaa cgtaaaaaga gctgaagtct
     4501 cagaaagact ccgaaacgct atcgatggcg gcggagatga tcttcttgaa aatgcggagt
     4561 acgaagccgc aaagaatgaa caatcattca ttgaaggcag aatcagtgat ttggaatatt
     4621 tgatcgcggt ggccaagata gtcgacagcc cggaaaaaat cggcgttgcg gatatcggtt
     4681 cgaagatcaa aattcaagaa gagggtagcg acgaaattga aacttatacg attgtcggag
     4741 caccggaagc ggaccccaaa gaaaataaaa tctcgaacga atccccgatc ggaaaagcgg
     4801 tgttgggaca ccataaaggg gatatcgtcc aggtctccgc tcccgtcggt tcttataacg
     4861 taaagattat cgatatcgaa taaactacgg caaatctcat tctgaaataa agggaatcat
     4921 ggattttaat gaattagaaa aagtcagagt acaaaaaatt caaaaactga aagaattagg
     4981 catcccagcc tatccgactc gttcttatat cagttcaaca attaaagaag catgtgaagc
     5041 gtttgaaaaa gcggaatcga caggatccga tcaaccgatc gaagtgacga tcgccggtcg
     5101 aatgcgttcc ttacggatta tggggaaatt ggcgtttact catatcgaag acggaacggg
     5161 aaggattcag ctctttttga gagttaatga actgggtgat cagcttcagt tttttaaaga
     5221 ttatttcgat atcggtgatt tcgtcgaagc ccacgggacc gttatcagga cgcgatcggg
     5281 tgaaatatcg cttcagacaa cttccattcg gatgctcgcc aaagctattt cgccgcttcc
     5341 cgcagcaaaa gaagaggtag tcgatggagt gaccgtcaag cataccgggt tgagcgatcc
     5401 ggaaatccga tatcgccaac gatatgccga tttggtggtg aaccccgaag tccgaaatat
     5461 cttccgaacg cgttcaaaga tcgtttcggc gttgcgagaa ttcttagaca gtcacgattt
     5521 tttggaagtt gaaacccctg tattgcaacc gatctatgga ggagctgccg ccagaccttt
     5581 cattactcat cataatcagc ttcatcagga tctttatttg cgtatatcgt ttgagttata
     5641 tctgaaaagg ttgttggtcg gcggactgga tcgcgtctac gagatcggcc gtgattttcg
     5701 caacgagggc gtctcttata aacacaatcc ggaatttaca cagctcgaat tttattgtgc
     5761 ttattttgat tatctgaaag ttatggattt cacggaacag atgatccgtt atgccgcaga
     5821 aaaagcactg ggaacgacca aattgaaata cggcgatgtt gaatttgatt tggcaaaacc
     5881 ctggaatcgg gtaaatatgc gggaagggat tctggaaaag accggaatcg atttcgaaaa
     5941 atatccgaca accgaatctt tggcagctgc aatgaatgaa aaggggattc agaccaagcc
     6001 ggggttgcaa cgcggaaaat tgatcaatca attggtaagc gattttttgg aaccggcttt
     6061 cctgcaacct acttttctat acgattatcc gtgggaagta tcccctttgg caaaaagaaa
     6121 accggaaaat ccacttatca ccgagagatt tgaaggattt attgccggaa tggaactttg
     6181 taacgctttt acggaactga atgatccgat cgatcaggaa gctcgttttc tggaaatggg
     6241 aagaacttat gcggaagatg acgaggaacg aaatccgatt gatgaggatt atttacgtgc
     6301 gatgcgatac ggcatgcctc cctgcggcgg tttcggaatg ggaattgatc gtttaacaat
     6361 gctactgacc aaccaaagca caattcgcga agttatttta tttccccatt tgcgtgaacg
     6421 agataaggat caggaagaca actgatgaat tttgccgttc tctgggattt agacggaaca
     6481 atttcggatt cgttccaatg tttttatatg gcattggaac gaattttgag tgaacagaaa
     6541 ccaacgatca aaataacacc cgaagaatat aaatctcagt atttcggcgc aacttttccc
     6601 agcattttgg atagaatgta tcaaggaaga ctctccgata cacaaatttg ggaattaaac
     6661 gaacgctatt tcaaatattc cgttgaattc gccagacagg gaaccgtctt tccgatcccg
     6721 ggtgtcgaat ctttcttgaa aaagttacac gccgctgaca tcaggatggc catcgcctca
     6781 tccagcacgg tcgagatgat ttttaccgaa ttggatgttc tgggattcat ttcctatttc
     6841 gacaatatta tttcggggaa tttgttgccg tccaagccgg ctccggatgt tttccaaatc
     6901 gcagccgcat ccatccgagc gcaaccttct caatgcgttg tgatcgaaga ttctgtcgcc
     6961 ggtattacag ccgcgaagaa tgcgggcatg cgcaccattg ccgttacaac caccaacccg
     7021 gctgataagt tcgggcaggc tgatcgggtg acggaagatt ttcgaaagtt cgatctcgac
     7081 gcgttaaaat ccctttttgt cactgatttt tcaaatttat gacagtaata tcgaatttat
     7141 gacattcgta atattcagat tcctttttgg aaacgaattt tctttttttt aatcaggatc
     7201 ctaaataatt cgatctaaag gaaggttttt ttggctcatt tcctcgtaaa aatgaccgaa
     7261 tcggattttc cggaataatt tcaaccaagc cgtcaatatc atcattcttt gaaatttctc
     7321 taaaaaagtc aatttttacg caaaaaatac gtcatcagta agccattttg gcttgacaaa
     7381 tatacccatt tttttaaact attgatatac ttttgaatta gtcaagcgga ataggtagaa
     7441 gcgcatttta ttccgttcta attctgaaag aaatttatta tggaggtaat aatggaaatc
     7501 gaaggatatt gtgtaaaatg caaggctaag cgtactattc agaaacctac cgccggtttc
     7561 aataaacgag gcagcgcagt tgtatacggt acctgcccgg tttgcggtac aaagatttat
     7621 cgaatcggtc gtaccgaatt acataaagga atggaagctc ccaaaaaata gagctttcta
     7681 aaaaagaaaa tgaagctgcc atgcgtttcg tatggcagtc attatttaaa agggaaattt
     7741 caaccgaaat atggatactt tggaactcga atcctttctc tccgatttgc cgatgggagg
     7801 aatcaaatat tttgaaatcg tcgattctac caatcgctat gcattggaat gggcagaaaa
     7861 aggtgcggaa gatttcagtt tggtagtcgc aaatgaacaa actgccggtc gaggccggat
     7921 ggcaagaaaa tggctgacaa cacctggcaa cagtcttgcg atgagcctga ttttgcggcc
     7981 gagccataaa gaacaaaaac agattcatct gttttcacca ttagccggat tggcagttgc
     8041 ggaatcattg aaaaatacct atggactgaa tccgttgatt aaatggccga atgacattct
     8101 gttggatcat aaaaaggttt gcggtattct ctgcgaagct gcatgggata aagacaagtt
     8161 aagaggcatg gtcatcggga tcgggttaaa cctgaaaaaa gaatcgatcc ccacagatga
     8221 cagccttcgt taccctgcga gttccttgga atcggcaggt tgcgattgct tggaagtttt
     8281 accgcatatc cgtaatattt tgcttgaatt ccgccgttta cgtccgattc tggcaacctc
     8341 tgaattcatc acaaaatggg aaagctacct ggcattcaaa ggagaacaag ttatcctaac
     8401 agcccccgaa aaagaccccg aaatcttcac attaatcgga ttggatgcag agggcaattt
     8461 gcatgtaatt gattccgccg gagatgaaaa attcttccaa gccggtgaaa tttccctcag
     8521 acctttcaaa tcataaaaaa tccctgacga aacaaaaaag gaaaagaacc gatcttttcc
     8581 ttttttgttt tgaattcatg aaggagttat tgtttcaata attcttcgag gatcgacttc
     8641 gtcaagacag ggtccgcttt cccgcgcatc tcccgcatga cctgaccgac gaaccagccg
     8701 atcaatgttt ctttcccgcc tttatacaaa cccacttcct ttggattatc cgttatgata
     8761 cgctcgcaaa ctttgcggat ttcgctgtcg tcactcacaa gaccgagccc cgattcttcg
     8821 acaatctctg cgggagattt gccggtttcc tcaactttat ccaccaacgt cttgccggtc
     8881 gatgtattga ttcttttgga atcaaccaac gaaatgatat ccgccagatc ctgcggagtg
     8941 attctcaact cggaaaccga gatacctttt tcattgacaa ctcgcagaac ttcattcatc
     9001 atccaattcg aaacacgctt attgtctttc ccgtaaatct tgcaagccgc ttcgaagaat
     9061 tcggaggtat ccctttctcc ggtcagaata gaggcatcat actcgggtaa acccaattca
     9121 ttaataaacc gttcacgccg ttggatcgga agctccggca tttttgcttt cacttcttcc
     9181 aggaattctt cggtaatgac aacaggcatc aggttcggct cacgaaaata acgataatcc
     9241 gccgctgttt ctttggaacg catcttccgc agttcctgag catcgttatc ccactcgatt
     9301 gtccattgct caaccttgtt tcccgcattc aattcacggg tctgccgttc aacctcggaa
     9361 ttaatcgctg cccgaacggc ttcgatcgaa ttgatatttt ttatctcgca ttttttccca
     9421 agttctttgg cgcctttcgg tcgaatcgag acattggcat cacagcgtaa atgggctttt
     9481 tccatattcg cttcggaaac accgatccaa cgcaataact gtctcagacg catcagatag
     9541 gtggctgcct cgtcggcaga atgtaagtcc ggtttcgtga ccatctcaac caggggtacg
     9601 ccgcaccgat tcatatcgat cagacggata tcgccttcat ttttcgtttt tccggcatct
     9661 tcttccatgt gtaaattatc gatatgaacg cgaattttcc ttccgtcatc cataaaaaag
     9721 tctaaatatc cgcctttacc gatcggcata tcattttggg taacttggta tccggccgga
     9781 agatccgcat aaaggtaatt ctttcgatca aagaaagatt tcctgttgat atccgcattc
     9841 attgccagac acagcatcag agctttttcg accattgcct tattggtccg gggaagtgtg
     9901 ccgggctgcc cggtgcaaac gggacaaata ttggtattgg gttcctctcc ccatgaatcg
     9961 gctttgcagc tgcaaaaagc tttggttacg gtatttaatt ggatatgggt ttcaagccca
    10021 atagttactt catattcggt catgattctc ttccccctta tcgtaaaacc accggtttga
    10081 ctttccgcca atcttcgtcc gacgtcgcct gttcgaaagc gtaagccgca cggagtattt
    10141 tggattcgca gtaatcatat ccggcagctt ggagtccgat cggtagacct tcggaactga
    10201 gtccgcaggg aaaactgatt ccgggagtac cggcaaaatt catcggcgaa gtgaactgat
    10261 cggcaaattg catcagaagc ggatctgtgg cggtgacccc accgaattga aatgccggtg
    10321 tcggcgtaga cggtgtcaat aacacatcca agcgatattt gccattcgga tcgaaggctt
    10381 cgtcgaaatc attacgtaac atggcacgtg ctcgttgggc acgcaaatag tacttttcat
    10441 aaaatttttc ctgactggca aaaagtcccg tcaggagacg gagtttcgct tgatacccga
    10501 agccttcccc acgggatttg tagtacaaat catacatatc ctcaaccggt cttttcgtgg
    10561 tatgcccgta tttgagcccg tcatatcgtt gtaaatttga ataagcctcg attcgggtta
    10621 taacgaaata cgccggaact gaatattttg tattggtcat cgggacgtct tcgatgattt
    10681 cggcgcccaa atctttatag attcgcagag ctttcctgac acaggattcg atttcgggat
    10741 cgatcgccgt ggaagaataa ttgccgtttt catccaaata agtaatcctc atataatccg
    10801 gagaaacgcc gattcgcatc cccttaacgc cttttttcag ttcttccgga taattatcca
    10861 cgggtactct tcccgtgaca ctgtcattcg gatcgaaccc ggcgatttcc cgtaatacag
    10921 ctgcggcatc ggcaaccgtg cgggtgatcg gtcccatcga atccattgaa gaggcaaacg
    10981 gaatcacacc gtaacgtgaa acgcgaccgt aagtgggttt caatcccacg gttccggtaa
    11041 atgccgccgg ttcgcgaata gacccaccgg tatcggtgcc cacagaaatg gcgccttcat
    11101 ttgcggcaac cgctgccgcg gaaccgcctg atgaccctcc cggaacatga tctaaagccc
    11161 acggattgcg cacggtcggt ttaaatgcgg ttgtttcgtt ggatccgcca aaagtaaatt
    11221 catccaaatt cgcttttccg aacagcaccg catccgcccg ttccaaccgc tgaataactg
    11281 tggaagtgta aggaggatga tacccatcca gaattctgga tgcggccgtc gtttgaatcc
    11341 cttttgtgca atagacatct ttcgccgaat aaggaacacc ggctaagggt cccaattcct
    11401 ctccggcggc tactttccga tccaccgctc gggctttttc gcgtgccgat tcttccgtga
    11461 tcaaaatgaa cgcatgcact ttttctttat cttccggttg ctcaatatac tcttccaatg
    11521 agcccggacg accatccact ttttttactt gcgccaacgt ttcgttcaga agttcttccg
    11581 ccgatatttt tttctctcgg atcagtcttg cttgatccaa agccgacata ctcgttaatt
    11641 cagtcatgat ttactccaat gtctgatgtg gaacatccgg cgaaacaata tatccgtctt
    11701 gtgtcctcgg tgcttggctg atgatatctt ccggatgctc aaaaggaatc caaacatcct
    11761 ctcgcaacgg tgcctggatt tcttccggaa acgggttccc atgaattaca gggggtaaat
    11821 tgccttctaa cggaatggcg gtcagttcgt gaattaccgt gagctggtca ttcattttgg
    11881 ctaaaattga ctcaaactct tcggtgctta attcaacttc cgcaacatgc acaaaatcat
    11941 cgagcatttc ttttgttatc gcatcattgt ccataaaatc acctttttta tatggaattt
    12001 tgtcgaaggg atcttcgaat aattgcaaat aggacctttt caagcaatca attcctaatt
    12061 ctaccaaata aagatgtgat taacagataa aagattcagg ttcagtgtca taaatttaca
    12121 agcatcattc gataaaagca gtgccgatac cgggctggtt tttttactca ttattccaaa
    12181 ggatgattca aaagttctat gctatgggaa aaatcaacgg aacggaaaaa gattgaaatg
    12241 ttataatcac cccaatggaa acccctcata ataacttgaa gaatatccaa aaagttctta
    12301 agtattgctt tatcttcatg atttttgttt cctatttgac atttttaaat cgagtcggca
    12361 aattaaacct ttttttcact caaaaagatt ttaaaaacat tcaatatgat gcgggatatg
    12421 cttggattgc ggatattaat gaccctaaaa tagcgatcgg ctataatctg ccggtaacat
    12481 tatatgagaa cgatctcaaa attttacgag atccaaaagc gactaccgaa tcggttcgag
    12541 aacaaggggg cggaaaattt gtaatttggg ataacggtac gatctgtttt tcaacttccg
    12601 ataattccaa ccccactcaa aatggcagaa cttatcgggt ggaatatcct cgaattgtcg
    12661 aaaagcggac ggtcagaatc atatttgctt ttaccttttt gctgggtttt ctttggatcg
    12721 gtttttcact cccggggaaa agaaagaaat tcgacttacg atcgacgatt cttggaaaga
    12781 atagaaaact ttttctcttt tctctgttgg cacttattcc ttttttgtat tttgcaatca
    12841 aatttttgct tccgcagccg ttgtggggag atgagctttt cacattaaaa aactacgttt
    12901 atgcaaaaga ctggtactat cccgcaactt tttatgatta tccaaataat catgtattct
    12961 tcagcctgat catttccctt tattcgaaat tcataaaaat tcgaactttt tgtgatgcgg
    13021 tacaaagtcc gtggaagatc agattactcc ttatttttct ttcgatcata acgattttct
    13081 ttacaacctt atcagcccgg aaaatcaata aatccgcggg agtgattgcg tccattcttc
    13141 tgagcacttc ccttcctttc tttctttgga ccactcaaat tcgcggttat ggattaagca
    13201 tgacactgat gagcattctg atttatctgt taatgcgtgt ccaaacttct ccgaatcgga
    13261 tttcttttgt cagcattgcc attgtttcag cccttttcgt atacatcatg cccacgaacg
    13321 cagtttacat tatcgcaatg ggtttttatt ttctttttaa gttgctgatt gatctcggaa
    13381 aatataaaga atccggggaa aacggaatta tttatcggat aaaaaggaat ccggaagcaa
    13441 aaattcttta ttcctttctt tggggcgctg ttttttcaat catgctttac tatccgattt
    13501 tggatcaaat gatgcatgta tatctgcccg aatcagggga aaaggttatc atgagccgcg
    13561 aactgttttt caggatattt cgcctcggaa ccgttgaaat aatccgatca tctttggtcg
    13621 ggcagcctct gctctcggct tgtattttga tcggtttctt tttattcttg cggaatcccg
    13681 gagagaataa gtctctgtca aggatggttt tcggcaccgg tctatgtctc ttaatcattc
    13741 ccgatttgtt cttctttctt ttaggttctg tcccctatgc tcgcaattct ttggcaattc
    13801 ttcccgtaat cattctgatt tttgcatccc tgattgcccc gatacctgat gcattatccg
    13861 atcctcgcct aaaaaatata agttttttgg tactattgat cagctccggt gctttctttt
    13921 ccgatcacct ggttcgtttg agcaaagaac ctctcagcta cggaaacaac tcactcaata
    13981 atctgacgga accttttttt ctcggggatc atctgaattt caatgaggtg ctgacggaat
    14041 tgaaagccaa aaatgctgcc ggtctgcccg ttttgttgaa atctcccgct ccctatttca
    14101 ttgaaagtat ctgcgattgc tatcagcggg tttgctatgg cgacggatcc ggtgaaggac
    14161 aaactttatt aaagaatcac gctccatatc tactgatcca aacggattgg aaagaatttt
    14221 ccgcaaattc ggaaaaatat ccggatcttt ctcgctgcac cacgattaat caagagaatc
    14281 gaacatcttt taaggttttt ctatgcaatc cataaaggaa aataatccgt gacaaaaaac
    14341 aaaatacttt ttccccttgc tatccttatt tgcctggctc tttttgccgt accgatcaaa
    14401 gttttttccg aagaatccat tgaagttgtt tttaatgatc catttggtaa aaatcacgat
    14461 ccggctattc aagatctgat tcttaatgct tttgaaaatg ccgagaatca catttacatt
    14521 gctatctata actttacgga taccgcttgc cgagacgctt tgatcgacgc ggtgaaacga
    14581 ggtgtggaag tccggctggt tatttattcg gaaaatgccg acaacagagt catcggggat
    14641 ttgacaaaga acgggataaa aattgtcaag tcgcaatcca aaggtttgat gcatgcgaaa
    14701 ttcatcgttg ttgattatga aacaacgatc agcgggtcgg caaatctgac acccgggagc
    14761 tttttttatg acaataattt tatgattttg attcacgaca gcaaagtgaa caaaattttt
    14821 caggatgaat tcaatgaaat gttcatcgaa aagaaattcg gcccgaccgg tcagcccaca
    14881 atacctccca ctgccgtctc tttgaaagac gggacaaaaa tcacggtgaa attctcaccg
    14941 gaggacggcg ttgaaaaaat tctcttatcg ctgattcggg catcccaaaa gagcattgac
    15001 gtgctggctt attctttcac ttcggataat ctcggtaatg ctttgattga tcgatatgat
    15061 gacggggtaa atgtggaagt tctgtttgaa aaagaaacgt caaatcaata cggcagtgaa
    15121 gcggcttatc tggttcggaa cggcattccc acttacatgg atgggctgga agacggattg
    15181 atgcatgaaa aagtgatgat cttcgatgaa tccgtggtcg cggcaggttc gtataacttt
    15241 acgcgggcag ccgaaacggt aaacgacgaa caaattttga tcatcgataa tcgcaaaata
    15301 gccgatcaat ttgaagaaga attcgaaaaa attttagaaa aggcaaatta atcaattttt
    15361 gaagaatatt tgcggatcat tccgataata tttgtgaaaa tacttattaa aaagaagttg
    15421 tcctctgaaa aaaggacagc ttctttttga atcattctct gagctttata gagcaaacgt
    15481 tgaactcgtc agcagtgtgt tttccgataa gattaatgct gtgactttat tgcccgccaa
    15541 actttttccg ggtaaattgg aagcgaatca atccgagacc cgatggcgtt cgctactgca
    15601 ttggcaattg ccggagcgac tccgtccgtc ggtatttcag ctaacgcttt tgcgccgaaa
    15661 ggaccggtct tttcaccgtc cccttcctga atgatatagc tttccatcaa cggcatttca
    15721 tccgcttgca gaatatggta atcaccgaat cgcgggttca acaaattccc ttggttgtca
    15781 taacacattt cttcacaaag ggcataaccg agtgcggttg tttgcccgcc ttccagctgt
    15841 ccgatagcgg tcgtcggatt aatcaccgtc cccgggctga ctgtcataac caatttaatg
    15901 ggagtcacct ggccggtttc gatatccact tccacttcgg cgaattcggt tccgaagacc
    15961 ggcggacact ccggagaaac atgggaagca taagccgcga tttgtcgctg atccttacga
    16021 tgcaggctgt gcaatgcgat ctcctccatg gtaacgtgat ccccattcgg agcgtaaacc
    16081 ttttcatctt tcagataca
//
LOCUS       DAOSMM010000052        15506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3825_length_15506_cov_19.080642, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000052 DAOSMM010000000
VERSION     DAOSMM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3825_length_15506_cov_19.080642"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1896
                     /locus_tag="PK898_07805"
     CDS             100..1896
                     /locus_tag="PK898_07805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOU19973.1"
                     /translation="MGKDQFAAVELLWDILNGKTKEVSKVTYFPQKPDMPQDAKINQP
                     FPRATPESQGIPSERLSAMIEELSRYPWLDVHHVLVIRNGKMICECSFSPYRPNIWHS
                     SYSLCKSITGLAIGMLYDKGKIHLDTPITEFFKDKDRSRMSFRQKKITVENLLTMTSG
                     VDFHEAGAYTGDDWVKGYLGASVREMPGEIFDYNSMNSYMLSAIVTAVTGQSLMDFLT
                     PRLWKPLGITNVFWETCPMGITKGGWGLFICPEDAAKIGQLILQGGIWKRRRIISEEW
                     LKTAAEKHVEVPPESGLGYGYHMWMADRPGSSAFNGMLGQNVLIYPDINMVIVTNAGS
                     NELFQRSTLIATIEKYLGAGFEPADPLPENESEYKKLLRIQSALSGEENDAFLQQLKG
                     WSGKILRRSNKPNPARYVKKLDGMSYALQKKNIGIFPLLLQVVHNNFTEGIQTLSFHY
                     QDEKFWISFLEGTEKIDLPVGFEEPARSTVKIHGEGYLVATGGEFAFNEDDILVLKLD
                     IAFLEEAVRRKIKIYFLEDSIEMKWDEVPGARLILKGVQYILTDVSDNFLIHTVSHRI
                     NSDLVIEMLRNAIQPVILGLPAEQEEPQQEQP"
     gene            2034..3665
                     /locus_tag="PK898_07810"
     CDS             2034..3665
                     /locus_tag="PK898_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PfaD family polyunsaturated fatty
                     acid/polyketide biosynthesis protein"
                     /protein_id="HOU19974.1"
                     /translation="MTNQSSFIDVSGMADHSLLSQKNMGEYIPLTPEKMKSYLSDLAK
                     PVFVIKNGKDAFLSDSVFLSSSANSWKALAYCFPFTPEMFGDADFKRSYGLKYALYGG
                     AMANAIASEEMVISLGNSGFMGSFGAGGMSLSAIEKAIYRIREALKDKPYAFNLINNP
                     FDSEMEQKTAELYIQNGIHVVEASAYLTINSSLVRYRAAGLKQLPDGSVEIGNKLIAK
                     VSRKEVASQFLQPAPEEILSRLLSEGKLTEEQVRLAKSVPMADDITVEADSGGHTDNR
                     PLVSILPAIIRLRDSFQAKYHFPHPVRIGAAGGISVPDATLAAFMMGAAYVTTGSVNQ
                     ACIESGASEYTKNLLAQAEITDVAMAPASDMFEMGVKVQVLKRGTMFAKRAQKLYDLY
                     QRYDSVEEIPAAEREKLEATVFKRTLDSVWEECQKFFSERDPKQLEKAAKDPKHKMAL
                     IFRWYLGLSSRWSSIGEKGREMDYQIWCGPSLGAFNDWVRGTYLEKPNNRRVADVSLH
                     LLTGAAYLYRIRLLESQGIRFSSDLRDYLPLQPLL"
     gene            complement(3669..4370)
                     /locus_tag="PK898_07815"
     CDS             complement(3669..4370)
                     /locus_tag="PK898_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOU19975.1"
                     /translation="MTIYLTFADFPPLESDFSAEYLSDFLGEQERIEWDRLKVPKRKR
                     EWLSARVIGKKLIHTSIPELEQVPFSKIQILKEPGGAPFVSVENGKRTRIEISFSHSH
                     EHVFAACSPDNIRFGTDLEWIEPRSKEFVEDFFTENEIRRVVSSDPDQADLISTVIWS
                     AKESVLKALRTGLRLDTRKIEIGFTTEPSDREGWLQLEIKSPSRLLWRREGDFVETFC
                     ILENPEQDLRWVTLR"
     gene            complement(4421..12805)
                     /locus_tag="PK898_07820"
     CDS             complement(4421..12805)
                     /locus_tag="PK898_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU19976.1"
                     /translation="MDKQKMVAVVGLGAILPDALDVPSFWNNIVSNKYSITEVPSDRW
                     NVDLYYDPDPAAADKTYSKIGAFVKGYRFDSLKNGIAIPPKVLEVMDITQQWAISASR
                     QALMDYGYPERKLNPERVAVIFGNADGGERHYRSTMRILLPETLEFLRSVPDFAQLPE
                     DIRSAIEKGLATNIHSSIPATSEDTLPGEISNIIAGRVANVFNFNGQNFVTDAACASS
                     MAALQAAAEGLIAHRFDAVLTGGIDRNMGPESYVKFSKIGALSADGSRPYAEGANGFV
                     MGEGAAVFLLKRLEDAERDGDKIYAVVCGIGSSSDGKGKGITAPNPIGQKLAIERAWQ
                     DAGIFPESVGLVEGHGTSTKVGDVAEVNSLNAIFGSSGLKNGSVVLGSVKSNIGHLKS
                     ASGAAGLLKTILSLHYRVLPPSANFTKPNPNIDFNNLPFRVNKQAEEWTVKEGNHRYA
                     GVSSFGFGGTNFHIVLEEYYQGIGGTSSSTYAIPIDIHSSTGNSTASFDRSNSEIKQE
                     AMKVPTESQEIDIDEIKRYVLTTVSEKTGYPVEMLDPDLDLEADLGIDTVKQAELFAT
                     IRNNYGISRQEDLILSNYNTISKLIDFIQESITSLPAQNTPSVTEQKEEAEIKTTPEN
                     MSSETAELKPYQGLFFASADSAAELAVDLRQKLTDLRAGMLPESHIPSEAERSRAERI
                     AIDYANIEELPAKLEKALTALDTDTAGSWKSAQAYGIYHGSGKPGKVAFLFPGQGSQY
                     VNMLRDLWDSEPIVRDTFLEADRIMTPILGRPLTAYIYTDGGEQEIKQAENELKNTAI
                     TQPAVLTANVAILRILQKYGIEPDLVMGHSLGEYAALVTAEVLTFAEALEVVCARGQE
                     MSKIKVDDPGCMAAVSAPIEKVEEVIRSIDDYVVIANINSPVQCVLGGTTKAIDTAMS
                     KFQAEGYQIVKLAVSHAFHTKIVAPASAPLREVIARMNVKPPLLPIVANVTGELYPAD
                     RESILDMLAKHVSSPVQFIKGMKTLYENGARIFIEVGPKRVLTGIANDNLKGKEDVTI
                     LATNHPRKGGKASFNEALCGIYAAGVIGSEPRHRETVVESVKNEVPTGSVQGEVFSFP
                     QGEQPVFGSVVISGVGLGLPGRNRHIFDDENVRSILDGEIRIDPLTEEKRRHLLEKQV
                     TRLIKSESGAVMENITGLDQTVKLAGQGGDFDVVEEFGFPQERAESLDIASKLAMAAG
                     IEALRDAGIPLVLHYKQTTKGTFLPDRWTLPESMRDETGIIFCSAFPGYNRMAEETDR
                     FYQNQILEKQLEQLRSIADLANSLNSNADNPLQKELKEKIAALESQIKENDYHFDRRF
                     VFRVLSMGHSQFAELIGARGPNTQINAACATTTQAVAIAEDWIRAGRCRRVVIISGDD
                     VTTSALFPWIGTGLFATGAATTEGNLRLAAIPFDKRRNGMIIGMGAAAMVVESEDATR
                     ERGVRAIGEILATCIANSAYHGTRLDMQHISEVMDRLLSTAEKRYGIQREKIASQMMF
                     MSHETYTPARGGSASSEIHALRSCFGDQANRIIIANTKGFTGHAMGAGIEDVVAMKAL
                     ETGKVPPIANIRDGFEPDPELGDLNLSQGGEYNPQYALRLGAGFGSQVAMSLIHKIPG
                     VGSRIDREKYHRWLSDISGYEQAESEVVKRTLRIKSEGIPARKPARSTWKYGDVPVVR
                     AAFETAEPIKASAQAPASIAVPPADSVSKPERSQFGKNQSAVLPNESTVSNESEIKTY
                     VLSIVSEKTGYPVEMLDPDLDLEADLGIDTVKQAELFAAIRSHYEIPRKEDLNLSEYN
                     TLAKVIDFVKENLSVKESTVAESEQPVREETKQADIKKEEIVSSLPAAAVNRDDIKQF
                     VLATVSEKTGYPSEMLDLDLDLEADLGIDTVKQAELFATIRTNYGIPRQEDLNLSNYN
                     TLSKVIDFVQNSLGTPVLSVQSEATEKVATAPEKTADKTQSVFSEAEKSEASAPKSSL
                     IRRAPVPVLFPRIDLCLPTEVRLQGSKIIIVGDHGKVAEALIEKLEAAGAQTRLTALS
                     KVREDLSTWGKEEKPDGLYFLPSLDPDPVWEVNHVRDWEEALTEMEEALYSIGILLPD
                     TAFLIGATRLGSFNGLMNCENPLNGIVTGFMKSLRRERPHQLVKVVDFNDQASPDYIA
                     GALISETLQDPVTAEIGREDDLRNGIALQECETVDTGTVTLQTGSTFVVSGGTGGVTG
                     VVIQDLAKATRGSFFLLGRTELPAEDDPDLLKLKRDRKGFGLELQERMRQAGEKVTPV
                     QIERQLLALERASATLEVMKTVEAAGGHARYIPCDVTNENSVINAIETIREATDKVDV
                     FIHAAGLDKSRKIESKPFEEFQQVIDVKAKGFKYIFNALENNHLLPDKVVFFSSVAGR
                     FGNSGQTDYGAANDLLAKYAQWLPKRYPGMQAVAIDWSAWAEVGMASRGNMPRLMEMA
                     GIDLLKPEEAAPMVRNELNLGQSGEVVIAGSLGILESSLNEKNCGMDIEKADAALRAG
                     TPIHRMFSHLTAFDSNSGIQLEGELDPAQLSYLRDHAINGIPVLPGVVGIEGFAIASK
                     HIASVLASTSKGFEIKKLEKIRFQAPLKFYGNKTRKIAWHAAAYRKPEGIVIKASLES
                     DIKRRDGTISHLNHFDSLVYLTQNPAEAESVTAAPKWGKRRSVSSEEIYKLYFHGPSF
                     QVLDAAQLSESAVLGKFNKQLMNVPADESVPYMKSLLIELCFQSAGLWEVGSTGVLSL
                     PQSVGNLSFYKQPINKGPIFAEVQPREHEGQISFDAKVVDAKGNVILELEDYRTSPLP
                     YSADKKLVEPIKSMVTEQSKDSQH"
     gene            13146..13862
                     /locus_tag="PK898_07825"
     CDS             13146..13862
                     /locus_tag="PK898_07825"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOU19977.1"
                     /translation="MVRKIIADSDRIVFFGGAGVSTASAIPDFRSPDGLYNQTPAGTY
                     SQETMLSHHFFIQHPQEFYENLRRNMLFPDAQPNAAHIALAKLEKQGKLRAIITQNID
                     GLHQKAGSENVIELHGNIDRFYCTGCQKAFQLNDILRFVDSVPKCDSCGAIIRPDVVL
                     YEETLDQEELMKAVDSISHAEVLIVGGTSLVVYPAAGLLQYYSGDKLILVNRSITPYD
                     QRANYVLHGDISKVLPELIR"
     gene            13954..14424
                     /locus_tag="PK898_07830"
     CDS             13954..14424
                     /locus_tag="PK898_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOU19978.1"
                     /translation="MSVENVKAYFDQYGISDRIREFEESSATVELAAGLLSCSPGEIA
                     KTLAFRLNGGCVLIVAAGDAKVDNSKFKAVFGSKAVMVKPEEAEELVGHAVGGVCPFA
                     VKEGVRVYLDQSLKRFETVYPAAGDSHSVIRLSIAELEKYSGAIGWIDVCKNWQ"
BASE COUNT         3874 a         3587 c         3692 g         4353 t
ORIGIN      
        1 cggatcggat tgataccgaa atgaaaaggg tcaattcgat tttaaataat tgaaggaatt
       61 ttcgggctgc ggtagaatgt gattgaaaag aggttattca tggggaaaga tcagtttgcc
      121 gccgttgaat tgctatggga tattctgaac gggaaaacaa aagaagtcag taaagtgaca
      181 tattttccgc aaaagccgga tatgccgcag gatgcaaaga tcaatcagcc gtttccgaga
      241 gcgacacccg aatcccaggg aattccgtcg gaacgtctct ccgcaatgat cgaagaatta
      301 tcgcgttatc catggctgga tgtccatcat gtattggtga ttcgaaacgg gaaaatgatt
      361 tgcgagtgca gtttcagccc gtaccgacca aacatctggc acagcagcta ttcgttatgc
      421 aaaagtatta ccggtttggc gatcgggatg ttgtacgata aggggaagat tcatttggat
      481 actccgatta cggaattctt taaagataag gaccggagtc ggatgtcttt tcgtcagaag
      541 aaaatcaccg ttgaaaatct gttgaccatg accagcggag tggacttcca tgaagcggga
      601 gcttataccg gagacgattg ggtgaaaggc tacttgggtg cgtcggttcg ggaaatgccc
      661 ggggagattt ttgattataa cagcatgaat tcctacatgc tgtccgcaat cgtaaccgcc
      721 gttaccggtc aatctttgat ggattttttg acccctcgtt tatggaaacc gctgggaatt
      781 acgaatgttt tttgggaaac ctgtccgatg ggaatcacga aaggcggatg gggccttttt
      841 atttgtccgg aagatgcggc gaagatcggc caactcattt tacaaggggg gatctggaaa
      901 cggcgacgga tcatttcgga agagtggctc aaaacggctg ccgagaaaca tgttgaagta
      961 ccgccggaaa gcggtttagg atacggttat catatgtgga tggcagatcg tcccggaagt
     1021 tcggctttca acggaatgct cggacagaac gtgttgattt atccggatat caatatggtt
     1081 attgttacaa acgccggcag taacgaattg tttcagcgca gcacactcat cgctacgatc
     1141 gagaaatatt tgggtgcggg ttttgagccg gctgatccac tacccgaaaa cgaatccgaa
     1201 tataagaagc tgttgcgaat tcaaagtgcg ctttccggag aggagaatga tgctttcctt
     1261 cagcaattga aaggctggag cggaaaaatc ttacggcgga gcaataaacc aaaccctgcc
     1321 cgctatgtta aaaagttgga cgggatgagt tatgcgttac aaaagaaaaa tatcggcatt
     1381 tttcctctgc ttttacaggt ggtacataat aattttacgg aaggaatcca aacgctttca
     1441 tttcattatc aggatgaaaa gttttggatt tcctttttgg aagggacgga gaagattgac
     1501 ctccccgtgg ggtttgaaga gccggcgaga tccactgtca aaattcacgg agaaggatat
     1561 ttggtggcta ccggcggtga atttgctttt aatgaagatg atattctggt ccttaaattg
     1621 gacattgcct tcttggaaga ggcagtacgc cgaaaaataa agatttattt tttggaagat
     1681 tccattgaaa tgaaatggga tgaagtaccc ggagcgcgat tgattctgaa gggagttcaa
     1741 tatattttga cggatgtctc cgataatttt ctgattcata cggtcagcca tcggattaat
     1801 tcggatttgg taatcgaaat gctcagaaat gccattcaac cggttatttt gggattaccg
     1861 gcggaacaag aagagccgca acaggaacaa ccgtaaaccc ggcttttcca aagcggtctt
     1921 ttcgcagcat tcttttcgtc atccttttga tacaattctc acgattcaca aaaaaataaa
     1981 aaaaccgcct gaaccttaag aaagtggctt aaaatacaaa agttaggtga aaaatgacaa
     2041 atcaatcttc ctttattgac gtttcgggaa tggcggatca ttctcttttg agtcaaaaga
     2101 atatggggga atatatcccg ttaactcccg aaaaaatgaa aagttatctt tcggatctgg
     2161 cgaaacccgt ttttgtgatc aaaaacggga aagatgcttt tctttccgac agcgtgttcc
     2221 tttcctcatc ggcgaattca tggaaagcct tagcttattg ttttccgttt acccctgaaa
     2281 tgtttgggga cgcggatttt aaacgatcct acggcttgaa gtatgctctt tatggcggag
     2341 cgatggctaa tgctattgct tcggaagaaa tggtgatttc cttgggaaat tcgggcttta
     2401 tgggatcttt cggagcaggc ggaatgagcc tctccgcaat cgaaaaagcc atttatcgga
     2461 ttcgggaagc tctgaaagat aaaccctatg cctttaattt aattaacaat cctttcgatt
     2521 ccgagatgga acaaaagaca gcggaactct acatccaaaa cggtattcat gttgtcgagg
     2581 cttccgctta tctgactatt aattccagcc tggtccgata tcgggctgcg ggattgaaac
     2641 aattgccgga cggttccgtc gagatcggga ataaattgat cgcgaaagtc tcacgaaagg
     2701 aagttgcctc tcagttcttg caaccggctc cggaagaaat tctttcccgc ctgctttctg
     2761 aagggaaatt gaccgaggaa caagttcgtc ttgcaaaatc ggttccgatg gccgatgata
     2821 taacggttga ggcagattcg ggggggcata cggataaccg gccgttggtg agtattctgc
     2881 cggcaatcat ccgtctgcgt gattcttttc aagcaaaata tcactttccg catccggtac
     2941 gcatcggtgc cgccggagga atttcggttc ccgacgcgac gctggctgcg tttatgatgg
     3001 gtgccgccta tgttaccact ggttcggtga atcaggcatg tatcgaatcg ggcgcttccg
     3061 aatatacgaa gaatttgctc gcacaagccg aaattacgga tgtcgccatg gctccggcaa
     3121 gtgatatgtt cgaaatgggc gtgaaggttc aggtgctgaa acgggggacg atgtttgcga
     3181 aaagagcgca aaaactttat gatctttacc agcgatatga ttccgttgaa gaaatccccg
     3241 cagccgaaag agagaaactg gaagcaaccg ttttcaaacg aactctggac tcggtttggg
     3301 aggaatgcca aaaattcttc tccgaaagag acccgaaaca gcttgaaaaa gccgctaagg
     3361 accccaaaca taaaatggca ttaatctttc gctggtattt gggtctttct tcccgttggt
     3421 cgagcatcgg cgaaaaggga cgggaaatgg attatcagat ttggtgcgga ccttcgttgg
     3481 gcgctttcaa tgattgggtg cggggaacct atctggaaaa accaaataat cgcagagttg
     3541 cggacgtctc gctgcatctt ttaaccggcg cggcatatct ttatcggatc cgcttgctcg
     3601 aatcacaggg catccgcttt tcttccgatc ttcgcgatta cttgccgctt caaccgcttc
     3661 tctgaccgtt agcggagggt aacccagcgt aaatcctgct ccgggttttc cagaatacag
     3721 aacgtttcga caaaatctcc ttctctgcgc cataagaggc gggaaggaga ttttatttcc
     3781 aactgcagcc agccttccct gtcggacggt tcagtcgtga atccgatttc gatttttctt
     3841 gtatccagtc tcaatccggt tctgagcgct ttcagcaccg attccttggc gctccaaatg
     3901 acggtggaaa tgagatcggc ttgatccgga tcgcttgaaa caacccggcg gatttcattt
     3961 tcggtaaaaa aatcttcgac aaattctttt gatctgggtt caatccattc caagtccgtg
     4021 ccgaaacgaa tattgtccgg agaacaagcc gcaaaaacat gttcatgcga atgactgaac
     4081 gaaatttcga ttcgagttcg tttcccgttc tccactgaaa cgaacggtgc gccaccgggt
     4141 tctttcaaaa tttgaatttt tgaaaaagga acttgttcca gttcgggaat ggaagtatga
     4201 atcaattttt ttccgattac ccgagcggat agccactctc tctttcgctt gggaactttc
     4261 agacgatccc attcgattct ttcctgttct ccgagaaaat ccgaaaggta ttctgcggaa
     4321 aaatcagatt caagaggcgg aaaatcggca aaggtcagat aaatcgtcat gaatagaaac
     4381 aaatcccccg acctttatta tagccggggg atttcgagaa ttaatgctga gagtcttttg
     4441 actgttctgt taccattgat ttgatcggtt caacgagctt tttatctgcg gaataaggta
     4501 agggagaagt tcgataatcc tccaattcga ggataacgtt cccttttgca tcgacaactt
     4561 ttgcatcaaa agaaatttgt ccttcgtgtt cccgcggttg gacttccgca aaaatcggac
     4621 ccttgttgat cggttgttta tagaaactta agtttccgac ggattgcggc agagaaagaa
     4681 caccggttga gcctacttcc cataaccccg cagactggaa acataattcg atcaacaaag
     4741 atttcatata agggaccgac tcatcggcgg ggacgttcat caattgctta ttaaatttac
     4801 ccaatacggc actttcggaa agctgagccg catccagaac ttggaaggaa ggaccgtgga
     4861 agtacagttt atagatttct tccgatgaga cggatcttcg ttttccccat ttgggagctg
     4921 cggtaacgga ttcggcttcc gccggatttt gcgtcagata taccagactg tcaaaatggt
     4981 tcaaatggct gattgtgccg tcgcgacgtt ttatatccga ctctaaactt gccttgatga
     5041 cgataccttc cggttttcgg taagcagccg cgtgccaggc gatttttctt gttttatttc
     5101 cgtaaaattt aaggggtgct tgaaagcgga tcttctccag ctttttgatt tcaaacccct
     5161 tgctggttga ggctaagacg gaagcaatgt gtttggaagc gattgcaaat ccttcgattc
     5221 ccaccacacc cggcaaaacg ggaattccgt tgatggcgtg atcgcgcaaa tacgataact
     5281 gagccgggtc taattcacct tcgagctgga ttccggagtt gctgtcgaaa gcggtaagat
     5341 gactgaacat acgatgaatc ggcgttccgg ctcttaaagc cgcatccgct ttttcgatat
     5401 ccataccgca gtttttctca tttagcgaag attccaaaat tcccaaagaa ccggcgatga
     5461 cgacttcacc gctttgcccg agatttaatt cattccgtac catcggggcg gcttcttcgg
     5521 gtttcaagag atcgattccc gccatttcca tcaggcgcgg catgttaccg cggcttgcca
     5581 tacccacttc cgcccaagcg ctccagtcga tggcaacggc ttgcataccc gggtatcttt
     5641 tgggtagcca ttgagcatat tttgccaaca aatcattggc ggcgccgtaa tccgtttgtc
     5701 cggaatttcc aaatctgccg gcgacggatg aaaagaatac cactttgtcc ggcaagagat
     5761 gattgttttc gagtgcgttg aaaatatatt tgaagccctt ggcttttaca tcaatgacct
     5821 gttgaaattc ttcaaacggt ttggattcga tttttcgact tttatccaat cctgctgcat
     5881 gaatgaaaac atccacttta tccgtagctt cccgaattgt ttcaatcgcg ttgataaccg
     5941 aattttcatt tgtgacatcg cacgggatgt atcgggcatg accgccggct gcttccaccg
     6001 ttttcattac ctctaaagtt gctgaggcac gttccaaagc gagaagttgt ctctcgattt
     6061 ggacaggtgt gactttttct ccggcttgcc gcattctttc ttgcaattcc aaaccgaacc
     6121 cttttcgatc tcgtttcagt ttcagtaaat ccgggtcgtc ttcggcgggc agctcggttc
     6181 tgccaagcag gaagaacgaa cctcgtgtgg cttttgccaa atcctgaata actacaccgg
     6241 tcactccgcc ggtccctccg cttacgacaa aagtactgcc ggtttgaagg gttaccgtcc
     6301 cggtgtcgac ggtttcacat tcctgtagag cgatcccgtt ccgcaggtcg tcttctcggc
     6361 cgatctccgc cgtaacggga tcttgcaatg tttccgagat caatgcaccg gcaatataat
     6421 cgggggatgc ttggtcgttg aaatcaacga cttttaccaa ttgatgaggc cgttcacgac
     6481 gtaatgactt cataaatccg gtgacaatcc cgttgagagg gttctcacaa ttcatcaggc
     6541 cgttgaaact gcccaggcgg gtggctccga taaggaaggc ggtatccgga aggagtatcc
     6601 cgattgaata gagagcctct tccatttcgg taagtgcttc ttcccaatct cttacgtggt
     6661 tcacttccca aaccggatcg ggatcaagag aaggaaggaa ataaagtccg tccggttttt
     6721 cttcctttcc ccatgtcgat agatcctccc gaacttttga gagcgcagtc aggcgggttt
     6781 gagctcccgc cgcttccaat ttctcgatta aggcttcagc gacttttcca tgatccccga
     6841 cgatgattat tttgcttccc tgcaaccgga cctcggttgg gaggcacaaa tcgattcgcg
     6901 ggaataaaac cggaaccgga gcacgccgga tcagtgaaga cttaggagcg gatgcttccg
     6961 atttctccgc ttcggaaaaa acggattggg ttttgtccgc ggttttttcg ggggcagtcg
     7021 ccactttttc ggtagcttcc gactgaacag ataggacggg cgttccgagg gagttttgaa
     7081 cgaaatcaat cacctttgac aaagtgttgt aatttgaaag gttcaggtcc tcctgtcgag
     7141 gaatgccata attcgttcgg attgttgcga aaagttccgc ctgcttgact gtatcaatgc
     7201 ccaaatcggc ttccaaatca aggtctaaat ccaacatttc tgatggatag ccggtttttt
     7261 cactgacagt cgccaatacg aattgcttga tatcatcccg gttcacagcc gcggccggca
     7321 acgatgatac aatctcttcc tttttaatgt cggcttgttt cgtctcttcc cgaacaggtt
     7381 gttcactttc tgcgactgtc gattctttta cgctgaggtt ctctttcacg aaatcaatta
     7441 cttttgccag cgtattgtat tcggaaagat ttaaatcttc cttcctgggt atttcgtagt
     7501 gagaacggat cgctgcgaaa agttcggctt gttttaccgt atcgattccc aaatccgcct
     7561 caagatccaa gtccggatcc agcatttcga cggggtatcc ggttttctca ctgacgatcg
     7621 aaaggacata tgttttgatt tccgattcgt tcgaaacggt tgattcattc ggaagaactg
     7681 ccgattgatt tttcccaaac tgacttcttt caggtttgga taccgagtcg gccggcggga
     7741 ccgcaatcga agccggagcc tgagccgaag ctttgatggg ttccgcggtt tcaaacgcag
     7801 cccgaacaac aggtacatca ccgtatttcc aagtcgaacg agcgggcttt ctggcgggaa
     7861 ttccctcgga ctttatccgt aatgtgcgct tgacaacttc cgattccgcc tgttcgtatc
     7921 cgctgatatc ggacagccag cgatgatact tttcccgatc gatacgagat ccgacaccgg
     7981 gaattttatg aatcaggctc atcgcaactt gggaaccgaa accggcccct aatctgagcg
     8041 catattgcgg gttatattct ccgccttgag ataaattcaa atcacccaat tccggatccg
     8101 gttcaaaacc gtctcgaata ttggcgatcg gaggaacttt accggtttcc aaggctttca
     8161 tggctacgac atcttcgatt ccggcaccca tggcgtgacc ggtaaatccc tttgtattgg
     8221 cgatgataat gcgatttgcc tgatctccga agcaggagcg cagcgcatgg atttccgaac
     8281 tggcactgcc gcctctcgcc ggagtataag tctcatgaga cataaacatc atttgcgaag
     8341 cgattttctc ccgttgaatc ccatatcgtt tttcggcggt ggagagtaga cgatccatga
     8401 cttcgctgat atgttgcata tccaggcgtg tgccatgata tgcactgttg gcaatgcagg
     8461 tagctaagat ttctccgatt gctctgacac cccgttcccg agttgcgtct tcgctttcga
     8521 caaccatcgc agccgctccc attccgataa tcatgccgtt tcgccgtttg tcaaaaggaa
     8581 tggcagctaa ccgtaaattt ccctcggttg tggcggctcc ggtcgcgaac agccctgtcc
     8641 cgatccatgg gaacagagcg gaagtcgtga cgtcatcgcc cgaaatgatg actacccgcc
     8701 ggcaacgacc tgcgcgaatc caatcttccg cgattgcaac cgcctgagtg gtggtcgcac
     8761 aagcggcatt gatttgagta ttcgggcctc tggctccgat caattccgca aactgggaat
     8821 ggcccatgga aaggacgcga aatacgaagc gccgatcaaa gtgataatca ttttccttta
     8881 tttgggattc cagagcggcg attttctcct ttaattcttt ttgaagtggg ttgtcggcat
     8941 ttgaattcaa cgaatttgcc agatccgcaa tcgagcgaag ttgctcaagc tgcttttcca
     9001 atatctgatt ttggtagaaa cggtctgttt cctcagccat tcgattgtag ccggggaatg
     9061 cggaacagaa aattataccg gtttcatccc gcatggattc cggtaaggtc caacggtccg
     9121 gcagaaaagt tcctttggtg gtctgtttat aatgtaaaac caatggaatt cccgcatctc
     9181 ttaaagcttc gattccggct gccatggcta acttagaggc gatatcgagc gattccgcac
     9241 gctcttgcgg aaagccgaat tcttccacca catcaaaatc accgccctga ccggcaagtt
     9301 tcacggtttg gtcaagaccg gtaatgttct ccatgaccgc accggattcg cttttgatca
     9361 agcgggttac ctgcttttcc aacagatgtc gccgcttttc ctccgtcaat ggatcgatcc
     9421 ggatttctcc gtccaggatg ctgcggacat tttcatcgtc gaaaatgtgg cgattcctgc
     9481 cgggcagccc cagaccgact ccgctgatta cgacggaacc gaaaacgggt tgttctcctt
     9541 gcgggaaaga aaatacttcc ccttgaaccg aaccggtcgg gacttcgttt ttgacagatt
     9601 cgacgacggt ttctcgatgt cgcggttcgg agccgatgac gccggcggca taaatcccgc
     9661 agagcgcctc gttgaagctt gcttttcctc ctttgcgggg atggtttgtc gccagaatgg
     9721 tgacatcttc cttgcctttc agattatcat tggcaatgcc ggttaaaact cttttcggac
     9781 cgacttcaat gaaaatccgt gccccgtttt catagagtgt tttcatccct ttaataaact
     9841 gaaccggaga agatacatgc tttgccagca tatccagaat tgattcacgg tcggccggat
     9901 acagttctcc ggtgacgttt gcgacaatcg gaaggagagg tggcttcaca ttcatacgag
     9961 cgataacttc tcgtaagggt gcgcttgccg gagcgacaat cttggtatgg aaagcatggg
    10021 aaacggctaa tttgacgatt tgataacctt ccgcctgaaa ttttgacata gccgtatcga
    10081 tggcttttgt cgttccgcct aatacacatt ggaccgggct gttgatattg gcaattacaa
    10141 cataatcatc gattgatcga atgacttctt cgactttttc aatcggtgcc gaaacggctg
    10201 ccatacaccc cggatcatcc accttgattt tgctcatttc ctgtccgcgt gcgcatacca
    10261 cttccaacgc ctcggcaaag gtcaggactt cggctgtcac taatgctgca tattcaccca
    10321 aactatgacc catgactaaa tccggctcga tgccgtattt ctgtaaaatt cgtaaaatcg
    10381 ctacgtttgc cgtgagtacc gcgggctggg taatcgcggt attttttaat tcattttcag
    10441 cctgtttgat ttcctgttcg ccaccgtcgg tatagatata ggcggtcaac ggacgtccca
    10501 agatcggggt catgattcga tccgcttcaa ggaacgtatc gcgaacgatg ggttcggaat
    10561 cccataaatc ccgcagcata ttgacatatt gtgagccctg tcccgggaag agaaaagcca
    10621 cttttccggg tttcccgctt ccgtgataaa tgccgtaagc ctgggcggat ttccaagaac
    10681 cggcagtatc ggtgtccaaa gcggttaaag ctttctccaa ctttgcgggg agctcttcga
    10741 tattcgcata atcgatggca atccgttccg cacgcgatcg ttccgcttct gacggaatgt
    10801 gggattccgg cagcattccc gctcgaagat ctgttaattt ttgtcttaag tcaactgcta
    10861 attcagcggc ggaatccgct gaagcaaaaa acaaaccctg ataaggcttc aattctgcgg
    10921 tttccgaaga catattttcg ggtgtggttt ttatttcagc ttcttctttt tgttctgtta
    10981 ccgatggggt attttgagcg gggagagaag taatagattc ctgaataaaa tctattaatt
    11041 ttgagatcgt gttgtaattg gataaaataa ggtcttcctg acgggaaatg ccgtaattat
    11101 ttcgaatcgt tgcaaaaagc tcggcttgtt ttacggtatc gatgcctaaa tccgcttcca
    11161 gatccaggtc cggatccaac atttcaacgg ggtatcccgt tttttcgctg actgtcgtca
    11221 gaacatacct tttgatttca tcgatatcga tttcttgaga ttctgtcgga actttcatgg
    11281 cttcctgttt tatttctgaa tttgaccggt caaatgatgc cgtcgaattt cctgttgagg
    11341 aatggatatc aatcgggatg gcataagtcg agctcgacgt gcctccgatc ccctgatagt
    11401 attcttctaa tacaatatgg aaatttgttc cgccgaaacc gaatgagctg actcccgcat
    11461 agcggtgatt tccctctttc accgtccatt cttcggcttg tttgttcact ctgaagggca
    11521 ggttgttgaa atcgatgttg ggattcggtt tagtgaaatt tgccgaaggc ggaagtaccc
    11581 gataatgaag agaaaggatg gtttttaaca gaccggctgc accggatgca cttttcagat
    11641 gtccgatatt tgattttacc gaaccgagaa ctacgctgcc gtttttcagc ccgcttgaac
    11701 cgaagatcgc gttcaggcta ttaacctcag ccacatcccc cactttcgtg gatgtcccgt
    11761 gaccttcaac caaaccgacg gattccggga atattcccgc atcctgccaa gctcgttcga
    11821 ttgccagctt ttgtccaatg ggattgggtg cggtaatccc ttttcccttg ccgtctgaac
    11881 tgctgccgat gccgcaaaca accgcataaa ttttatcgcc gtctcgttcg gcatcctcca
    11941 atctttttaa taggaagacc gccgcgcctt cacccatgac aaaaccgttg gctccctcgg
    12001 cataggggcg ggaaccgtcc gcactcaaag cgcctatctt actgaattta acgtaggatt
    12061 ccggtcccat atttcgatcg ataccgcctg tcagaacggc atcgaatcta tgagcgatta
    12121 atccctcggc agcagcctga agcgctgcca ttgaggaagc acaagccgca tcggttacaa
    12181 aattctgccc gttgaaattg aagacattgg caacccgtcc ggcgataatg tttgaaatct
    12241 cccccggtaa agtatcttcc gaggttgccg gaatggaaga atgaatgttt gtcgccaggc
    12301 ctttttcaat ggctgatcga atatcttccg gcaactgtgc aaaatccgga accgatcgga
    12361 gaaattccaa cgtttccggc aacagaattc tcatggttga ccgataatgc ctttcgccgc
    12421 cgtctgcgtt gccgaaaatg acggcgacac gttccggatt cagttttctt tccggatatc
    12481 cgtaatccat aagagcttgt cgactggccg aaatagccca ttgttgagta atatccatta
    12541 cttccagcac tttcggcgga atggcgatcc cgttcttgag agaatcaaac cggtatcctt
    12601 tgacaaacgc gccgattttg ctgtaagttt tgtctgctgc ggcaggatcg ggatcgtaat
    12661 agagatccac attccatcgg tccgatggaa cctcggtgat tgaatatttg tttgaaacaa
    12721 tgttattcca aaacgaagga acatccaatg catcaggtaa gatcgctccc agacctacta
    12781 ccgcgaccat tttttgctta tccatgctta aatctcctta ttgatgctga attttgattg
    12841 caaatattga acacgtctat gaatcggatt ttttgtgtca ttaaaatata tttgctcaaa
    12901 attcttactt ctgaatatat cagaaacctt tttcgaattt ttaaaagaaa tgttagttat
    12961 tgtactactg ttgacttttt ggttatacga cgctaatcta aaatataaaa aagattatga
    13021 cttttaaaac gatttatgag gtgttttctg ccattaaatt atttcggatt aaaaagataa
    13081 aatatttgat aaagtaacta tagatgatga atataaagga aaaaatggaa acggatacct
    13141 tttcaatggt tagaaaaata atcgcggata gcgatcgtat tgtgttcttt ggcggtgccg
    13201 gagtatccac cgcctccgct atcccggatt ttcgttcgcc cgacggatta tataatcaaa
    13261 caccggcggg aacatattct caagagacta tgttgagcca tcattttttc attcagcatc
    13321 cgcaagaatt ttatgaaaac ttgcggcgaa atatgttgtt tcctgatgcg caacccaatg
    13381 cggcacatat tgcgttggcg aaattggaaa aacaaggcaa attgcgggca attattaccc
    13441 aaaatattga cgggctccat caaaaagccg gttctgaaaa tgtgattgaa ttgcatggga
    13501 acattgatcg attttattgc acaggctgtc aaaaagcatt tcagttaaac gatattttgc
    13561 gattcgtaga ttcggtcccg aaatgtgatt cttgcggtgc gatcattcga ccggacgtgg
    13621 ttctttatga ggagactttg gatcaagaag aattgatgaa agcggtggat tccatttctc
    13681 atgcggaagt tctcattgtg ggcggcactt cgttggtggt ttatccggcc gccggtttgt
    13741 tacaatatta ttcgggcgat aaactgattc tggtgaatcg ttctattact ccatatgatc
    13801 aaagggcaaa ttacgtgctt catggggata tttcgaaagt attgccggag ttgatccgat
    13861 aagtatttta tgtatggttt ggtcgagaac gtttatttgt aaacgggttt ctgtatctga
    13921 agatgaaata caactcagtg gaggagatca tgcatgtcgg ttgaaaatgt gaaagcatat
    13981 tttgatcagt atgggatttc cgatcgaatc cgggaattcg aagaatccag cgcgaccgta
    14041 gaattggctg ccggattatt gtcttgcagc cccggcgaga ttgcaaaaac tttagctttt
    14101 cgtctgaacg ggggatgtgt ccttattgtg gcggcggggg atgcgaaagt tgataattca
    14161 aaatttaaag ctgtttttgg atcgaaagcc gtgatggtaa aacctgagga agcggaagaa
    14221 ctcgtcggac atgcggtggg aggagtttgc ccattcgccg taaaagaggg tgttcgggtc
    14281 tatttggatc aatcgcttaa acggtttgag acggtatatc ctgccgcggg agacagccac
    14341 agcgtgattc ggttatcgat tgctgaattg gagaaatatt ccggtgcgat aggctggatt
    14401 gatgtttgca agaattggca ataaggaacg attttttatt tcggattata tttgcattgt
    14461 ctttttgtag gggacggcgt ccttgcgaag cacgccgagt ctacgaggcg caaacgtccc
    14521 gtattggctt gcaaaaaatc aagataattt taccgataat ttgttttcat atttgtgcca
    14581 ttttccctca ccctcccctc gtcctatggg agaggatttc tttaccgata atcgataatt
    14641 tttttgtagg ggacggcgtc cttgcgaagc acgccgagtc cacgaggcgc agacgtcccg
    14701 aatcggcttg cgaaaaatca agataatttt accgatgatt tgttttcata tttgtgctat
    14761 ttaccctcac ccttccctct cccggagggc gagggtttct ttgccgatga tggataattc
    14821 tgttgtaggg gacggcgtcc ccgacgtccc gaattggctt gcataaaatc aagataattt
    14881 taccgatgat ttgctttcat atttgtgcca tttaccctca ccgtcccctc tcccgcaagg
    14941 cgaggggatc cgaatcaaaa tatttttgct gatgatatat gaccatattt atgcaataaa
    15001 ccctcaccct gaccctctcc cacagggcga gggaatcctt aatcaagata attttaccga
    15061 tgatttgttt tcatatctac gccattttcc tctcaccctg accctctccc acagggcgag
    15121 ggtttctttg ccgatgatgg ataattcttt tgtaggggac ggcgtcctcg acgtcccgta
    15181 ttggcttgcg aaaaatcaag ataattttac cgatgatttg ttttcatatc tgcgccattt
    15241 accctcaccc tgaccctctc ccacagggcg aggggatccc gaatcaaaat atttttgctg
    15301 atgatatatg aacatatcta cgctatttcc ctcaccctga ccctctcccg cggggcgagg
    15361 gaatcccgaa tcaaaatatt tttgctgatg atatatgacc atatctgcgc cattttccct
    15421 caccctgacc ttttccacag gcgaggaaaa acggaagacc ggtggacaac ttgtggaaaa
    15481 atcggtggag aaaatgcgca aaaggc
//
LOCUS       DAOSMM010000053        15267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_3910_length_15267_cov_16.268867, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000053 DAOSMM010000000
VERSION     DAOSMM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_15267_cov_16.268867"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PK898_07835"
     CDS             <1..978
                     /locus_tag="PK898_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19979.1"
                     /translation="IDKSPSTQFLYHDRFLIPSFWGISLALTAFIAVYLKGFRKKIIF
                     LSLLCGICVFFQIKNSIYYRNAWESQQNFQWQMKWRAPDLKANTAVLGDAIIASFMGG
                     WADGAMVFEMYGKNDGYNPTSYWYLVVGEGDYKDEFDQKLPITYVNKIYDFKAQFGDF
                     LVLTKPEWDRCLWVLDEADLKNPYIQPVTKNLIQYQNKSRILYDSAYKMPEGVFGSDY
                     RHDWCYYYENAAREVDLKNYENALELYEEATAANMDMKNPVELTPFIRSAAMTGRWDL
                     AAELTEKGSAQPHITWEYYKNLWELLFRDTPDSAARANAFKSIQEYIRQ"
     gene            1281..>1681
                     /locus_tag="PK898_07840"
     CDS             1281..>1681
                     /locus_tag="PK898_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU19980.1"
                     /translation="MEKKSIRLMLCFLMMVSIFVGIGSVKSAFAEDTVTLEFSQWWEP
                     ELPQGAFRSLMDKFEEQNPGIKVELLSGPYSSTKEQIVAGAATGTMSDVVGLDGAWVN
                     DFVKQGAITDLSKLMTDAGYDSSALAAQIQI"
     assembly_gap    1682..1781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1782..2474
                     /locus_tag="PK898_07845"
     CDS             <1782..2474
                     /locus_tag="PK898_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU19981.1"
                     /translation="LSLEVPNGIQNDVMSWVWASGKSMLKDGKPDVTNSDVKSAVEFI
                     KGLYDAGVIAPGAFTMKEQDKVEEFTNGRVGMMIDSLAHINTIRERNPELKFSLAAVP
                     AEDGYDGKRGLPYASWGIGIAENSQHKAEAWKLVEFLMSEEVNSELSTIANAFPGNVN
                     SVPDFVKTDELFGSAFEIYQKGYPANEFVGLPVAEDLMRAFDEQFQLMLEGDQTVDEM
                     LQKVQEAWLKEF"
     gene            2585..3463
                     /locus_tag="PK898_07850"
     CDS             2585..3463
                     /locus_tag="PK898_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU19982.1"
                     /translation="MNTFKKKAAPYLYLSPTIILMIVLMLIPIVMVISYSVMNNVIMN
                     KNPIYIGLGNYKTILTDEVFHTTIYNTLFFTVGSVVMHMVIGLLFAMLLNSRLFSTAT
                     KSFFRVIYILPWVFTAAIIAILWRLLLNPNGIINYFLTQAGIIHTKVEWLGSRDTALY
                     ALTFINIWSGYPFYMISLLAGLQGISQDLYEAAEVDGANAYHQFISITIPQLKPIILS
                     MAMLDCIWTMQQFPLVWMTTGGGPIHATEMLSTYTYKLAFSKYEFSLASASAVIILIF
                     SMVLASVYVRRQKASV"
     gene            3465..4307
                     /locus_tag="PK898_07855"
     CDS             3465..4307
                     /locus_tag="PK898_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU19983.1"
                     /translation="MLSKKAKHNFAKTFALVLLILGAIYAGAPIIWMFLCSFKPNTEI
                     FAYPPTFISKTFSLTAYKKILTDSVKVRFFLNSYFVSTAVTLLTLIVSIFAAYSFSRF
                     EFKMKKTLNMIIISVQAVPPITLLIPYFGLIVTLKLYNTYWALILTYMVFTLPYAILM
                     MTGYFNTLPRELDEAVMIDGGSRSVALWRVLFPISIPGIISVGMYTFMQAWNEYLFAL
                     TLTKTVNMRTVPIGIQQLMGQHSYEWNEMMAMSILGCMPVLILFLFFQRYFIGGMTAG
                     SVKS"
     gene            4369..5229
                     /locus_tag="PK898_07860"
     CDS             4369..5229
                     /locus_tag="PK898_07860"
                     /inference="COORDINATES: protein motif:HMM:NF006042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOU19984.1"
                     /translation="MLMNMADLLAVANKNYFAVPAFNISDYSMLNGIFEASEEKNAPL
                     IIAIHPDELKFIGVEMVKAIIEKAYKTKLPVCIHLDHGADFNQVILAIQNGFTSVMID
                     GSALPFEENIAICKKVVEAAHAVNVSVEGELGTIGSTDAQAEQGMNKIIYTNPADAVK
                     FVRETGIDSLAIAIGTSHGLYPKGMKPELKLDLLKEIKSIISIPLVLHGGSNNRDDEI
                     REAVNLGINKINISSDIKAAYFMKMREVLNDLSLREPNVIQPSCIAAMKEVAYQKIDL
                     FLATGKASLY"
     gene            5251..6435
                     /locus_tag="PK898_07865"
     CDS             5251..6435
                     /locus_tag="PK898_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent glucokinase/phosphofructokinase"
                     /protein_id="HOU19985.1"
                     /translation="MTKDIVLGLGNNIDYEIEWDSAIFENLVHEFGIEYSEIHPVGEV
                     QTQRDLIISILSYMKEGKGGECFVRSSQIIEDFSKAFRKKITLGGTSVRAAIAMSKIG
                     YSSALHLVTMNDDVRRLLPPDCEYICSAPEENSYPHLIIQFTQNHTIRVQDKIIRPKQ
                     ANRIIYDNDLDNILMRLDPRLSQLLLNAKVFLISGFNAMQDQSLLEDRLEKLLISMKN
                     LPKDALVFYEDACFYNKNFSRIVRDKLLGHIQIFSLNEDEFEGYIGRKINLLDPLEVL
                     QSLEVLCKLIPVPKIVLHTQYWALAYGRDADSLKKALKGGITMGGTRYRFGDDFTRQN
                     YFEMEKSAPQKEGAQFSDRINAMKQGEICCLPCVEVNDKNVTTIGLGDAFVGGFLPAL
                     VH"
     gene            6454..7299
                     /locus_tag="PK898_07870"
     CDS             6454..7299
                     /locus_tag="PK898_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOU19986.1"
                     /translation="MEINQTKTVIETINENYDQIFSAEKKVADFILSFPELAVNANVS
                     ELANYSNVSDATVIRFCKHIGYQGYYQLRIILSRDLGRKQVYESEDLSGEDQSVNGLF
                     KMFASNFLKIGQNLDDETILACASVIRNCNQAHVIAVGNTSPLALYMGFRLGRLGIKS
                     SYGMIPEYFMNHINLAEKNDVVIAISQSGCSKLVVQGMELAKEKGLVTIAITAHKYSP
                     VSRLADYLLLSTVGEKSFYYYKSYSHLYEAGVVEALLYYVTNEDKINDKSADKPEIIL
                     SESKL"
     gene            7407..8171
                     /gene="infC"
                     /locus_tag="PK898_07875"
     CDS             7407..8171
                     /gene="infC"
                     /locus_tag="PK898_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOU19987.1"
                     /translation="MVYCLFGFSVGIYQNSADNKEGVIISTQDFRVNETIRVPEVRVI
                     DDKGNNVGVIPIKEALQLARDADLDLVEVSPNADPPVCRVMDFGKFLYERTKKEKEAR
                     KTQTKIEVKEIRLRPKTNEHHRSFKTRDARRWLEDGMKVRVTVRFRGREITYPELALE
                     DLKEIAEELSDIAAIEQLPAMEGRVMSMVLAPNIKKVVRKKTESSSAAVNTAKNSKNS
                     KKSSLPKEMPEEEIDSDDIEADEEEDDEIVSDTDLE"
     gene            8266..8496
                     /locus_tag="PK898_07880"
     CDS             8266..8496
                     /locus_tag="PK898_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOU19988.1"
                     /translation="MPRKQTTGKLKLKTHKATSKRFRMTGGGVLVRTKGGKSHFRRRT
                     SKRTKALLDEMIPVQGKSIIDRVKHLAPYMEK"
     gene            8585..8935
                     /gene="rplT"
                     /locus_tag="PK898_07885"
     CDS             8585..8935
                     /gene="rplT"
                     /locus_tag="PK898_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOU19989.1"
                     /translation="MARVKSGPYGHQRHKRLLKRTEGYYATRRKLYRRANETLLKGMW
                     YAYRDRRVRKRELRKLWIARINAAARINGTTYSRLIAGLKKADINLNRKMLADIAVRD
                     PQTFSKIVEQAKVN"
     gene            complement(9003..9788)
                     /locus_tag="PK898_07890"
     CDS             complement(9003..9788)
                     /locus_tag="PK898_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU19990.1"
                     /translation="MKLISQSQIRDFQTCPQKYYLSHILKLSWPAPPSPLFKKLEEEA
                     ALGEAFHLFVHRIIQGLNPEESAFETENKTIAKWIDNFLRNYPLPQDAVLYSERELTN
                     LDEDILWIGKFDVIATLQNKILIFDWKTSQKAGKVLWLEESPQTRLYRFLLYQNRQLY
                     SYDGTDHMKPEQIEMIYWFVNFPNSPIHIGYSEKQFAADRQYLKEIAAKINSDKAENY
                     PKTSIEKVCNYCQFQTYCKRGFPDQSDSEYEPDWNNLPLTEEL"
     gene            complement(9785..12070)
                     /locus_tag="PK898_07895"
     CDS             complement(9785..12070)
                     /locus_tag="PK898_07895"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOU19991.1"
                     /translation="MTFIPRSTQAEILKYTGGTMGISAVPGSGKTHTLSALAAKLVEQ
                     MMTNKTSNPFNGTEREVLIVTFSNSAVSNFSARIAKFLSEKGLVPGIGYRVRTLHGMA
                     TDIVRGRAEGYGLDPDFNILDETAANMLLRRAVEQWAADDNAATFNRYIKKDIKEEKQ
                     KGILSGKWLDDITTVAGSVISQAKDYQLSPEMIADRLKSFPGEDFSLLRMVSSIYSRY
                     QTMLRSYPALDFADLMFYAAQILENDPVYLNILQDRWPFILEDEAQDSSLIQEKVLRL
                     LTKRNGNWVRVGDPNQAINETFTTADPKFLKEFLKEADRTVDLAHSGRSTVSILKLAN
                     QLIRWVQEEHPTKYCRDALVKPYIKTTPKNDPQQNPKDEPKRVVIDGEKHSPIEEIGM
                     VSEAAAIHARNYPQETVAILVPNNNFGTKFVDELKKYPVEVIEILKSTRSTRYTADQI
                     AKVLYWLSIPTNNERCRRLFSSLYHHAAEGDFYLSLDHGQIATTKIKSIENLEDFFYP
                     NSNRQFEELILSWELPEIVIHSLFSFRHLLKKWLEARTLRIDQLILLIAQDLYSDPDS
                     LGIASQLSRIALQMTQADPSMQLNAVAENINKAAANADLYPELSKPDIQFDPNLYQGK
                     IVVTTFHKAKGLEWDQVYLTSCNNYEFPAGSGYTPRGYREYIRSQFFYIRDQLDIQAE
                     SLEQLRILFNAEPSEIYREGKGSVQSYNSVVSERLRLLYVGITRAKKGLHISWNSGHS
                     EQGQEALAIKFLRECSGGKKK"
     gene            complement(12067..14322)
                     /locus_tag="PK898_07900"
     CDS             complement(12067..14322)
                     /locus_tag="PK898_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19992.1"
                     /translation="MVASIRLSEAQNELIALPLSEHIFLDGKAGCGKTTAAIERLRKI
                     LNSPVSGSEVLVLLPSKTSSQKYYETYSVEGKRATVNTYNGLIQKAIKLFWPVVAEEA
                     GFASPEKSPIFLTIETSQILMAELIKPKIEAGYFSALRMPLSRIYNQIQIALHKCAAA
                     EFSFDSYAQRMRSSWSGDSAMLTVFDQVQECGNLFRKVCFENNLLDYSIQVEIFQNFL
                     LPSDRFQKWLKTNYHHLIYDNIEEDIPAAHRFAEILGESCESVLMVMDHLGGFRNFMG
                     SDTNSAFRLSEKCSVKRTFNDSFICEPAIRAMIHAIENPWIPTETLSANPKTAYIFQS
                     FHSYPVMIHQAAQDVAELINRQNVEPSDIAIIAPLLSDVLYTALERELRIQNISCYIH
                     RPSRPLIQEKETKGFLVLIALLHPEWGILPRMLEICEMLQIFIPSLDPIRAQILVSHG
                     FQVQTGGKFQYYTLKPFSEFSVSSQNRIGQKHRTCYETLRNWIRENISASEAPDLTLT
                     RFFHEILVGEEFAARSGEVAMISLGIQKVIESMKKYRLVIEKLENPLINWSDYLKLVW
                     EGMISARYYEEWYSQPKDSVLISLSSSYALMNRPVSYQIWLNAGSPRWWERIYGQITN
                     DYILSQDWKMGDLWDANSVYEKNDENMHRLVLSLLSRCRKQVRVYTSELNESGQDQKS
                     KLLYVFSELSYRFNRDSSVWPAAMIQEDVSLSTDTGFYPGIDDIPNESKTQISDFYPE
                     EDIKHWEENME"
     gene            14489..>15267
                     /locus_tag="PK898_07905"
     CDS             14489..>15267
                     /locus_tag="PK898_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU19993.1"
                     /translation="MNKKHRIYVSLLFFITVLLFSECLSVGAFYQEAAEPPYSGVNET
                     DWAALQAIYNKMTPYGQANSGWATGGNPCDPAWPGITCENGAVVKLDFEGRDFFCSIP
                     EEITQLSNLREIRMVNMGLRGIIPENLFGMQNLQTVNFSHNLLTGPLPQGSNQTLQTL
                     IIEDNKWTDQKQQELSARPDLSLCESVVQYPPEKNINMDPGLDGMIPSSLGYFPRLTK
                     LDLSGNMLEGEIPNDLINLNSLSSIDLSDNNPGNPLTISDQA"
BASE COUNT         4508 a         2904 c         3338 g         4417 t
ORIGIN      
        1 atcgataaaa gcccttccac gcaatttctt tatcatgatc gttttttgat cccgagtttt
       61 tggggaattt cgttggctct aacggctttt attgctgttt acctcaaagg atttcgaaaa
      121 aaaattattt ttctgtctct cctttgcggc atttgtgtat tttttcagat aaaaaattca
      181 atttactatc ggaatgcttg ggaatcccaa caaaacttcc agtggcaaat gaaatggcga
      241 gcaccggatc tgaaagcgaa tacagcggtt ttgggagatg cgattatcgc ttcatttatg
      301 ggtggttggg ctgacggtgc aatggttttt gaaatgtacg gaaaaaacga cggttataat
      361 ccgacatctt attggtattt ggtggtagga gaaggcgatt acaaggatga attcgatcag
      421 aaacttccga tcacctatgt caataagatt tatgacttca aagctcaatt tggagacttt
      481 ttggtattga cgaagccgga atgggatcgt tgcctatggg ttttggatga agcggatcta
      541 aagaatccat atattcaacc ggttacaaaa aatctcatac aataccaaaa taaaagtcgg
      601 attttatatg actcggccta taagatgccg gaaggtgttt tcggttccga ttatcgtcat
      661 gactggtgct attattacga aaatgcagcc agagaagtgg acctgaagaa ttatgaaaat
      721 gcgttggaac tttatgaaga ggcgacagcg gcgaatatgg atatgaagaa tccggtggaa
      781 ctaaccccct ttatccgatc cgccgcgatg accggacgat gggatttagc cgccgaatta
      841 accgaaaaag gcagcgctca acctcacata acctgggaat actataaaaa tctttgggaa
      901 ctgctgtttc gtgacacgcc cgattcagcc gcccgggcaa atgcttttaa aagcattcaa
      961 gaatatattc gtcaatagtt atttgtaatc aatccttttg agtttctacc aatataaaaa
     1021 tacagaaaaa acggttttcc gtggtaggaa aaatcgttac gaatgttttg tttaaaagtc
     1081 atcattagta aatggaattt cataaaaaaa tattatttca taatataacg ctgaatatct
     1141 cccaaatacc tctaaattat ctctgaataa ctgaaatata attaaaaata aaatttcatt
     1201 tcatgaaaat cattgactta tttaaccatg catgatatat tcttccaaac aaaatttaat
     1261 ccaatgaaag gaattatttt atggaaaaga agagtattcg tttgatgctg tgttttttga
     1321 tgatggtttc gatttttgtc ggaatcggaa gcgtaaagtc agcttttgcg gaagatacgg
     1381 taacgcttga gttttctcaa tggtgggaac cagaacttcc tcaaggtgca tttagaagtc
     1441 tcatggacaa gtttgaggaa cagaatccgg gaattaaagt ggagttatta agtggtccct
     1501 attcttcaac caaagagcag atcgttgccg gagcggctac cggcaccatg tctgatgttg
     1561 tcggtttaga cggtgcttgg gtaaatgact ttgtgaagca aggagctatt accgatttgt
     1621 ccaaattaat gaccgatgcg ggatatgaca gcagtgcttt ggctgcccaa attcaaataa
     1681 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgtctctt gaggttccga
     1801 acggtattca gaatgatgtg atgtcctggg tttgggcatc cggtaaaagc atgttgaaag
     1861 atgggaaacc ggatgttaca aacagcgatg tgaagagtgc cgttgaattc attaaaggct
     1921 tatatgatgc gggtgtcatc gctccgggag cttttacgat gaaggaacag gacaaggtgg
     1981 aagaatttac gaacggtcgg gtagggatga tgatcgattc attagcccat atcaatacca
     2041 ttcgggagag aaaccccgaa ctgaaattca gtcttgccgc agtacctgcc gaagatggat
     2101 atgacggtaa aagagggttg ccttatgctt cctggggaat tggaatcgcc gaaaacagtc
     2161 agcataaagc cgaagcttgg aaactggtgg aatttctgat gagcgaagaa gtcaattcgg
     2221 agttatcgac tatcgcaaat gcattccccg gcaatgtcaa ctccgtccct gattttgtca
     2281 aaaccgatga actattcgga agcgctttcg aaatttacca aaaaggatat ccggctaacg
     2341 aatttgtcgg attgcctgtc gccgaagatt tgatgagagc ctttgacgaa caattccagc
     2401 tgatgttgga aggggatcaa acggttgacg aaatgcttca aaaggttcag gaagcttggc
     2461 tgaaagagtt ttagactcgt tgtgaattga tttagagtta ctgaccacgt gaatccccat
     2521 aaaaattcat gtggtcagta aaattgtcag aaaataggga aggaataacc ggaattcgaa
     2581 aaaaatgaac accttcaaaa agaaagctgc gccttatctt tacctgtctc cgacaatcat
     2641 tctgatgatt gttttaatgt tgattcccat tgtgatggtt atcagttatt ccgtgatgaa
     2701 taacgtcatc atgaacaaaa atccgatata tatcggattg ggaaattaca agacgatatt
     2761 aacggatgaa gtttttcata caacaattta caacaccctt ttttttaccg tcggaagtgt
     2821 tgtaatgcat atggtaatcg gattattgtt cgcgatgctt ttaaacagca ggcttttttc
     2881 aaccgctacc aagtcgtttt tcagagtgat ttacattctc ccatgggttt ttaccgcagc
     2941 gatcattgcg attttatggc gacttttgtt aaatccgaac gggattatca attatttcct
     3001 tacacaagcg gggataatcc atacaaaagt tgaatggtta ggatccaggg ataccgcatt
     3061 gtacgcattg acatttatta acatttggtc cggctatccg ttctatatga tcagtctgct
     3121 tgccgggcta caggggattt ctcaagatct gtatgaagcg gcggaagtgg acggagccaa
     3181 tgcataccat cagtttatat ccatcacgat tccgcagtta aagccgatta tcctcagtat
     3241 ggcgatgctg gattgtatat ggacgatgca gcaattcccg ttggtatgga tgacgaccgg
     3301 cggcggcccg attcatgcca ccgaaatgtt aagcacatat acctataaac tggcattcag
     3361 taaatatgaa ttttcattgg cgtcagcgag cgccgttatt attttgattt tttctatggt
     3421 cctcgcatcc gtttacgttc gaagacagaa agcgagcgtt taacatgttg tccaaaaaag
     3481 ccaaacataa ttttgctaaa acgtttgctc tcgttctttt aattctcggt gcgatttatg
     3541 cgggcgctcc gataatttgg atgtttttat gctctttcaa acccaacacc gaaatctttg
     3601 catatccgcc gacttttatc tcgaaaacct tttctcttac cgcatataag aaaattctta
     3661 ccgattcggt aaaggtacga ttctttttaa acagttattt cgtttcgacc gctgtaacac
     3721 tgctcacttt aatcgtttca attttcgccg catacagttt cagccggttc gaatttaaaa
     3781 tgaaaaagac tttaaatatg atcatcatca gtgttcaggc tgttcctccg atcacgctct
     3841 tgatccctta ttttggtctt atcgttactt taaaactcta taatacctat tgggctttga
     3901 ttctgaccta tatggtgttt acgcttcctt atgcaatttt gatgatgacc ggatatttca
     3961 atacgttgcc gagggaactg gatgaggctg tcatgatcga cggcggaagt cgttcggtag
     4021 cgttgtggag agtacttttc ccgatttcta ttccggggat catttcggtc ggaatgtata
     4081 cttttatgca agcttggaat gaatatttgt tcgcattaac actgacaaaa acagtaaata
     4141 tgcgaaccgt gccgatcgga attcaacaat tgatgggcca gcattcttat gaatggaacg
     4201 agatgatggc catgagtatt ttaggctgta tgccggtttt gattttattc ctgttcttcc
     4261 aaagatattt tatcggcgga atgacggccg gatcggtaaa aagttaaaat agataaagat
     4321 agaaaataaa aatactaaaa tttataaaat attagaggat aatacaatat gttaatgaat
     4381 atggctgact tactggcggt tgcgaataaa aattattttg ccgttcctgc tttcaatata
     4441 agtgattatt cgatgctaaa cggaatattt gaagcttcgg aagaaaaaaa tgcaccttta
     4501 attattgcga ttcatcccga tgaattgaaa ttcatcggtg tagaaatggt taaagcgatt
     4561 attgaaaaag cgtacaaaac aaaactgccg gtatgtatcc atttggatca cggagctgat
     4621 tttaaccagg ttattcttgc cattcaaaac ggctttactt ctgtaatgat cgacggatcc
     4681 gctcttcctt ttgaagagaa cattgcgatt tgcaaaaaag tagtcgaagc agcgcatgcc
     4741 gtaaatgttt cggtagaagg ggaattagga acaatcggat caacggatgc tcaagccgaa
     4801 cagggaatga ataaaatcat ttataccaat cccgcggatg cggttaaatt tgttcgggaa
     4861 accggaatcg attcgcttgc aatcgcaatc ggaacatctc acggccttta tccaaaagga
     4921 atgaagcccg aattaaagtt agatttgctg aaagaaatta aatcgatcat ttcgatcccg
     4981 ttggttttgc atggcggttc aaacaatcgt gacgatgaaa ttcgggaagc ggtcaatctg
     5041 ggaattaata aaatcaatat ttccagtgac attaaagcag cctattttat gaaaatgcgg
     5101 gaagttttga atgatctcag cttacgggaa cccaatgtga ttcaaccttc ctgtattgct
     5161 gcgatgaaag aggtagccta tcaaaagatc gatctctttc ttgccaccgg gaaagcatcc
     5221 ttatattaat tcctgatttc atacgaaaat atgacaaaag atattgttct tggtttggga
     5281 aataatattg attatgaaat cgaatgggat tcggctattt ttgaaaattt ggttcatgaa
     5341 tttggaatcg aatattcgga aatccatccg gtcggagagg ttcaaactca aagagattta
     5401 attatttcaa tcttgagtta tatgaaagaa ggaaaaggag gggagtgctt tgtccgatct
     5461 tcgcaaataa tcgaagattt ttcaaaagct tttcggaaaa agattacctt aggggggaca
     5521 tcggtgaggg ctgcaatcgc aatgagtaaa atcggataca gttcagccct ccacttagtc
     5581 accatgaatg atgatgtccg gcggttgtta ccgccggatt gcgaatatat ctgcagcgct
     5641 ccggaagaaa attcttaccc gcatctgatt attcaattta ctcaaaacca tacaatccgg
     5701 gttcaagata agatcattcg accgaaacag gccaaccgta ttatttatga taacgatctc
     5761 gataatattc tgatgcgctt agacccgcgc ttgagtcagt tgttgctgaa tgcgaaagtt
     5821 ttcctgatat cgggatttaa tgccatgcaa gatcaaagtt tattggaaga tcggttggaa
     5881 aaattattaa tcagcatgaa aaacttgccg aaagatgcgt tggtttttta tgaagatgcc
     5941 tgtttttaca ataagaactt cagccgaatt gtcagagata aattgttggg gcacattcaa
     6001 atattcagtt taaacgaaga tgaattcgag ggttatatcg gtcgaaaaat aaatttgctg
     6061 gatccccttg aagttttaca atctttagaa gtcttatgta aacttattcc tgttccaaaa
     6121 atcgttttgc atacccaata ttgggcgtta gcttatggaa gggatgccga ttctctcaaa
     6181 aaagctttaa aagggggaat aaccatggga ggtactcgct atcgattcgg cgatgacttt
     6241 acccgtcaga actatttcga aatggaaaaa agcgctcctc aaaaagaagg ggctcaattt
     6301 tcagatcgga ttaatgcaat gaaacaaggt gaaatctgtt gtttgccatg tgtggaagtt
     6361 aatgataaaa atgtaactac aatcggactc ggagatgcct ttgtcggcgg atttctacct
     6421 gcactggttc attaattcta ttcagagggg tcaatggaaa taaaccagac taaaactgtt
     6481 atcgaaacaa ttaatgagaa ttatgaccaa atcttttccg cagaaaaaaa agtggcggat
     6541 tttattttga gttttcccga actggcagtt aatgcaaatg tctcagaatt ggcgaattac
     6601 agcaatgtca gcgatgcgac tgtcattcga ttctgcaagc atatcggata tcagggctat
     6661 tatcagttac gaattatcct ctcaagagac ttgggacgaa aacaggttta tgagagtgaa
     6721 gacctgagcg gagaagatca atccgtcaat ggtttgttca aaatgtttgc ctctaatttt
     6781 ctgaaaatcg gacaaaacct tgatgacgag acgattttag cctgtgcaag cgttatcagg
     6841 aattgtaacc aggcgcatgt gatcgcagtc ggaaatacaa gtcctctggc tctttatatg
     6901 ggatttcgat tgggaaggct ggggataaaa agctcatacg ggatgatccc cgaatatttt
     6961 atgaatcata tcaatcttgc cgaaaagaat gatgttgtaa ttgctatttc tcaatccggc
     7021 tgttcgaaac tggttgttca aggtatggaa ctcgcgaaag aaaagggatt ggttaccata
     7081 gcgattaccg ctcataaata ttcccccgta agcagattgg cagattattt gctcttatct
     7141 accgtcggtg aaaaatcttt ttattattac aaatcatatt ctcatttata tgaagccgga
     7201 gtagtcgagg cattattata ttatgtaaca aatgaagata aaatcaatga taaaagcgcg
     7261 gataagccgg aaataattct ttcggaatca aaattataaa aaatcgattt caattttgag
     7321 aaatcatttt aaagattgag aactcttcat aaaatacatt tcaaaaatgg caataatcat
     7381 ttttggtttg cggatatatt aaggatgtgg tatattgttt attcggattt tcagtcggaa
     7441 tctatcagaa ttcagccgac aataaggaag gagtaattat cagtacacaa gattttcgtg
     7501 ttaacgagac gatcagagtc ccagaagtgc gtgttattga cgataaggga aataatgtcg
     7561 gggtaatccc gatcaaggaa gcgttacagc tggcaagaga tgcggatttg gatttggtgg
     7621 aagtatcacc caacgccgat ccgccggttt gtcgggtaat ggatttcgga aaatttctct
     7681 atgaacggac taaaaaagaa aaagaagcgc gaaagaccca aacgaagata gaagtgaaag
     7741 agattcgttt gcggcctaaa acaaatgagc atcatcgttc ctttaagacc agagatgcaa
     7801 gacgttggtt ggaggacgga atgaaggtgc gcgtgactgt aaggtttcgt ggacgcgaaa
     7861 ttacttatcc tgaattggct ttagaagatt tgaaggaaat tgccgaggaa ttaagtgata
     7921 ttgcagctat cgagcaactg ccggcgatgg aaggaagagt aatgagtatg gtgcttgccc
     7981 cgaacatcaa aaaggttgtt cgaaaaaaga cggaaagctc ttctgctgcc gttaataccg
     8041 caaagaattc gaagaattca aaaaaatctt ctttaccgaa agagatgccg gaagaggaaa
     8101 tcgattcgga tgatattgag gcggatgaag aagaggacga tgaaattgtt tccgacacag
     8161 atttggaata atctgtgcct aaaacagaaa ataagagtat aataagcccg ctaagtaaat
     8221 gaaagccgtt attggctcat ttgattttat atgaaggagt cggccgtgcc acggaagcag
     8281 acaacaggaa aattaaagct gaagacccat aaagcgacat caaaaagatt tcgcatgacg
     8341 ggtggtggtg ttttggttcg tacaaagggc ggaaaaagcc attttcgccg ccgaacttcc
     8401 aaacggacaa aggcactatt ggatgagatg attcccgttc aaggtaagag cattatcgat
     8461 cgggtgaagc atctcgctcc ttatatggag aaataatcgt tttttgatta gtgcggctgt
     8521 cgggtgtaca caacggatct gaaaagatcg acgcccgggg gatcgcaaag gagtaattta
     8581 tcgtatggca agagtaaaga gtggtcctta cggacaccag cgccacaagc ggttattgaa
     8641 acgaaccgaa ggttattatg caacgcgtag aaagctctac agacgtgcaa atgaaacact
     8701 cctgaaaggg atgtggtatg catatcgtga tcgtcgagta cggaaacggg aattgcgtaa
     8761 actttggatt gcaagaatta atgcggctgc ccgaataaac ggaacgactt atagccgatt
     8821 gattgccgga ttgaaaaaag cggatatcaa cctgaatcgg aagatgctgg cggatatcgc
     8881 ggttcgtgat ccgcagactt tcagcaaaat cgtcgagcag gcgaaagtca attaaaatca
     8941 atacatcaaa gaattctctc gatataaaat aagacagctg ctaaatcggc agctgtctta
     9001 ttttataatt cttccgtcag cggcagatta ttccaatccg gctcatattc cgaatctgat
     9061 tgatccggga agcccctttt gcaataggtt tgaaattggc aataattgca caccttttcg
     9121 atggatgtct tcggatagtt ttccgcttta tcgctgttta tttttgcagc aatctctttc
     9181 aagtactgac ggtccgccgc gaattgtttt tctgagtacc cgatatggat gggggaattc
     9241 ggaaaattaa caaaccaata aatcatttcg atttgttcgg gcttcatatg atccgtcccg
     9301 tcgtaagaat aaagctgtcg attttgatac aaaagaaatc gataaagacg agtctgcgga
     9361 ctttcttcca accataaaac tttccctgct ttttgtgatg ttttccaatc aaaaatcagt
     9421 attttattct gcaatgtcgc gataacgtca aatttaccga tccagagaat atcttcatca
     9481 aggtttgtca gctccctctc cgaataaaga acagcgtctt gcggtaacgg gtaattccgc
     9541 aggaaattat cgatccattt tgcaatggtt ttattttccg tttcaaacgc tgattcttcc
     9601 gggttcagcc cctgaatgat ccggtgtaca aataaatgaa aggcttcccc caaggctgct
     9661 tcttcctcga gctttttaaa aagaggcgag ggtggcgccg gccagctgag tttcagtata
     9721 tggctcaaat aatatttttg cggacaggtt tgaaagtctc gaatttgtga ttggctgatt
     9781 aatttcattt cttttttccc cctgaacatt cccgcagaaa ttttattgcc aaagcttcct
     9841 gtccctgttc ggaatgtccc gaattccatg aaatatgcaa tccttttttt gcacgtgtaa
     9901 taccgacata taacaagcga aggcgttcgg atacgacgga attataggat tgaacactgc
     9961 cttttccttc ccgataaatt tcggagggtt cggcattaaa tagtatgcga agctgctcca
    10021 atgattccgc ttgaatatcc agttgatcgc gaatataaaa gaactgggac cggatgtatt
    10081 cacgatagcc tctcggcgta taaccgcttc ccgcaggaaa ttcatagtta ttacagctgg
    10141 tcaaataaac ttgatcccat tcaagccctt ttgctttatg aaaggtggta acaactattt
    10201 tcccttgata aagattgggg tcaaactgaa tatcaggctt actcaattcc ggatagagat
    10261 cagcgttggc ggctgctttg ttgatatttt cagccaccgc atttaattgc atggacggat
    10321 ctgcctgagt catttgaagt gcgattcggg ataattgact tgcgataccg agcgaatcgg
    10381 gatcggaata caggtcttgt gcgattaata aaatcaattg atcgatgcgc agagtccttg
    10441 cttccagcca tttttttaat agatgacgga atgagaaaag ggaatgaatg acgatttcag
    10501 gtagttccca ggataagatt aattcttcaa attgccgatt cgaattcgga taaaagaagt
    10561 cttcaagatt ttcaattgat tttatcttag ttgttgcaat ttgaccgtga tcaaggctca
    10621 aataaaaatc gccttccgcg gcatggtgat aaagagatga aaacaatctt ctgcagcgtt
    10681 cgttattggt aggaatggaa agccaatata aaacttttgc tatttgatcg gcggtatatc
    10741 gagtgctccg ggtactcttg agaatctcaa tcacttcaac cggatatttt tttaattcat
    10801 cgacaaattt cgtgccgaaa ttgttattgg ggaccagaat tgcaaccgtc tcttgagggt
    10861 aatttctggc atgaattgcc gccgcttcac tgaccatacc gatttcttca atcggagaat
    10921 gtttttcccc gtcaatgacc actcgtttgg gctcatcctt cggattttgc tgtggatcat
    10981 ttttgggagt agtcttgatg taaggtttga ccagtgcatc tcggcaatat tttgtgggat
    11041 gttcttcctg aacccaacgg ataagttgat tcgcgagttt tagaatgctg acggtggatc
    11101 ggccggaatg tgccaaatca accgtccgat cggcttcttt caaaaactct ttcaggaatt
    11161 tcggatccgc tgtggtaaat gtctcattga tcgcttggtt gggatcaccg acgcgaaccc
    11221 aatttccgtt tcgctttgtg agaagacgca gcactttttc ctggatcaaa gaactgtctt
    11281 gggcttcatc ctctaaaatg aaaggccacc gatcctgtaa tatattcaaa taaacgggat
    11341 cgttttccag tatttgtgcg gcgtaaaaca ttaagtcggc aaaatcaagc gccgggtagg
    11401 atctcagcat cgtttgatag cgtgaataaa tcgaagaaac catccgaagc aacgaaaaat
    11461 cttctccggg gaagctcttt aatcggtcgg cgatcatttc cggagagagt tggtaatctt
    11521 ttgcctgaga gatgacactt ccggcgacgg tagtgatgtc atctaaccac ttacccgaca
    11581 atataccttt ctgtttttct tcttttatat ctttttttat atatcgatta aaagtagccg
    11641 cattgtcatc ggctgcccat tgctcaaccg cccgtcgtaa aagcatgttt gctgcggttt
    11701 catccaagat attaaaatcg ggatccaaac cgtagccctc tgctcttcct cggacaatat
    11761 cggtagccat gccgtgaagt gttctgacac gataaccgat tccgggaacc aatccttttt
    11821 cggagagaaa tttggctatt cgagcggaaa aatttgaaac agcggaattt gaaaaagtga
    11881 caatcaaaac ttcccgctcc gttccgttaa acggattcga tgttttgttc gtcatcattt
    11941 gttcaaccaa cttcgctgcc aaagcggata gggtatgggt tttaccgctt cccggtacgg
    12001 cggaaatccc cattgtaccg ccggtatatt taagaatctc agcttgagtg gatctcggaa
    12061 taaaggtcat tccatattct cttcccaatg cttgatatcc tcttccggat aaaaatcaga
    12121 aatttgtgtt tttgattcgt tcggaatatc atcgatcccc ggataaaatc cggtatcggt
    12181 tgaaagggaa acatcttcct gaatcatcgc cgccggccac accgagctgt cacgattaaa
    12241 acgataggac aattccgaaa aaacataaag caatttggat ttttgatctt gtccggactc
    12301 atttaattcg cttgtgtaaa cccgaacctg ttttcgacag cgactgagga gtgataagac
    12361 caaccgatgc atattttcgt catttttttc ataaacggag ttggcatccc ataagtctcc
    12421 cattttccaa tcttgtgaga ggatataatc gtttgtaatt tgtccgtata ttctttccca
    12481 ccatcgaggt gatccggcat ttaaccaaat ttgataagaa acaggccggt tcatcagggc
    12541 atacgaagag gataatgaaa ttaataccga atctttgggt tgtgaatacc attcttcata
    12601 ataacgggcg gaaatcattc cctcccagac cagttttaaa tagtctgacc aattaatcag
    12661 aggattttca agtttctcaa tcaccagccg atatttcttc atcgattcga ttaccttctg
    12721 aattccgaga ctgatcattg caacctcacc gcttctcgct gcaaattctt cgccgacgag
    12781 aatttcatga aagaaacggg ttaaggttaa atcaggggct tcgcttgcgg aaatattttc
    12841 tcggatccaa tttcggaggg tctcataaca ggtacgatgt ttttgaccga tgcgattttg
    12901 ggacgatacg gaaaattccg aaaaaggctt cagggtgtaa tattgaaact ttccaccggt
    12961 ctggacttga aaaccatggg aaaccaaaat ttgtgcccta atcggatcca aagacggaat
    13021 gaagatttgc aacatttcac aaatttccaa catacggggc agtattcccc attcggggtg
    13081 gagcaatgcg attaatacca gaaagccttt cgtctctttt tcctgaatta atgggcggga
    13141 aggtcgatga atataacagg atatattctg aattcgcaac tcacgctcca acgcggtata
    13201 taagacatcc gaaagcaaag gggcgattat cgcaatgtcg gaaggttcaa cattttgacg
    13261 gttgattaat tcggcgacat cctgtgccgc ctgatgaatc attaccggat aggaatgaaa
    13321 cgattgaaag atataagctg ttttcgggtt tgcggaaagc gtttcggtcg gtatccacgg
    13381 gttttcaatc gcatggatca tggcccggat agccggttcg caaataaaag agtcattaaa
    13441 tgtgcgtttt accgaacatt tttcggaaag acggaaagcg gaattggtat cactccccat
    13501 aaaattacgg aatccaccca aatgatccat gaccataagg accgattcac aagattcgcc
    13561 gagaatttcc gcgaatcgat gagctgcggg gatatcttct tctatgttgt cataaatgag
    13621 atgatgataa tttgttttca gccatttttg gaatcggtca gacgggagga gaaaattctg
    13681 aaagatttca acttgaatcg aataatccaa gagattattt tcgaagcata cttttcgaaa
    13741 aagatttccg cattcctgta cctggtcaaa aacagttaac atggcggaat ctccactcca
    13801 gctcgatcgc attctttgcg catagctatc gaaagaaaat tctgctgccg cacatttatg
    13861 tagagcgatt tgaatttgat tataaattcg actcaaaggc attcggagcg cgctgaaata
    13921 gccggcttcg attttcggtt taatcaactc tgccataaga atctgagagg tctcgatggt
    13981 tagaaaaatc ggactttttt ccggagaagc aaatcccgct tcttccgcca caaccggcca
    14041 gaataattta atggcttttt gaatcaatcc gttgtaagta ttgactgtcg ctcgttttcc
    14101 ctcgactgag taagtctcgt agtatttttg ggaagatgtt ttggagggca gtaaaaccaa
    14161 cacttcggaa ccgctgaccg gcgaatttaa aatcttccgc agcctttcta tggctgcggt
    14221 tgtttttccg caccccgctt ttccgtccaa gaaaatatgc tcggaaagcg gcaatgcgat
    14281 taattcattt tgtgcttcgg ataaccgaat tgatgccacc atgatcaaat tgagtatacc
    14341 ttgaaatttc tcgaattcgc gagggaacat taacgaataa aaaacgtata aaaaagagag
    14401 aaaatgtttc tctataataa cgtatctttg tcaggtagaa ttagaaggaa gaaagaaact
    14461 cgtgggaaat tcggaagaag agggaaaaat gaacaaaaag cacaggatct atgtctcttt
    14521 gttattcttt atcacggtat tgctattttc agaatgttta agcgtcggtg ctttttatca
    14581 ggaagctgct gaaccgcctt acagcggagt gaatgagacg gactgggcag ctttacaagc
    14641 tatctataac aaaatgacgc cttatgggca agcaaattcc ggttgggcta ccggcggaaa
    14701 tccttgtgat ccggcttggc ccggaattac ctgtgagaac ggtgcggtag ttaaactcga
    14761 tttcgaaggc cgagatttct tttgttccat cccggaagaa attacccaac tttcaaattt
    14821 aagggaaata cggatggtga atatgggact ccgcggcatt attcccgaaa atctgttcgg
    14881 catgcaaaat cttcaaacag ttaacttttc acataactta ttgacgggac ctttaccgca
    14941 aggttcgaat caaacacttc agacattaat tatcgaagat aataaatgga ccgatcaaaa
    15001 acaacaagaa ttatcggcac gtccggactt atccctctgt gaaagcgtag ttcagtatcc
    15061 gccggaaaaa aatataaata tggatcccgg tttagacgga atgattcctt cttccttagg
    15121 ttattttcct cgattgacaa aactggattt gtcgggaaat atgcttgaag gggaaatccc
    15181 gaatgattta atcaacctga attccttaag ttcgattgat ctgagtgata ataatcccgg
    15241 taatccgtta acaatttccg accaggc
//
LOCUS       DAOSMM010000054        14968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4017_length_14968_cov_10.713874, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000054 DAOSMM010000000
VERSION     DAOSMM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 14968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14968
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4017_length_14968_cov_10.713874"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..2032)
                     /locus_tag="PK898_07910"
     CDS             complement(11..2032)
                     /locus_tag="PK898_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU19994.1"
                     /translation="MYNNTNPNDNSKDNNDFIAELVKQIALTEKEISEKNKIIQDIYS
                     TQSWKLAYKIERFYSKVAPPLSFRNRIIRSIFHFCLLIYHNLYKLFRKNISFESYSYN
                     QWIQKNEPLYRKTLQKNLNQNSATLISVVMPVYKTPTKILTETIDSVLNQDYTKWELC
                     IANGSPKDAKIANLVENYSKKDPRIKVLNLDKNLGIAGNTNAAIKIAEGDFIAFLDHD
                     DLLAPFALSAVINALHQYPDTDVFYSDEDKLNEDGIFRYGPFFKPAYSPDFLRAVNYI
                     CHLLVVRKSLGDQIGWLRDGFEGAQDFDLVLRAIEKARFVTHIPQILYHWRAMEGSTA
                     LSLESKSYATTSGIKAVQEHIDKLGLKGKVIQGPFPTSYKILYDNFEKSPLVSIIIPN
                     HNHGEDLTKCVDSIINGSTYKNIEILIIENNSTEDTVFDIYKKLETYENVKILKYNYP
                     FNFSKINNFAIHSAKGEILLFLNNDTEVITEDWLEQMLAFAIRPDVGAVGAKLFYPDG
                     TIQHGGVIVGLGGIAGHAYKGKPKIESEGILRLIVPQNYSAVTGACLMIKKSIFIEIN
                     GFDPAFQLAFGDIDLCLKIQEKGYRNVWTPYAQLYHFESKTRGYEDTPEKIERFTFEK
                     MIIKKKWHDKLEEGDPYYNPNLTLNSEDCSIDPSPRFPQPRSLKSEFFR"
     gene            complement(2142..3338)
                     /locus_tag="PK898_07915"
     CDS             complement(2142..3338)
                     /locus_tag="PK898_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU19995.1"
                     /translation="MSETARELLTALDGQRIINTHCHHLDDSFQNELNLESLLRNAYV
                     SWCGVDFDETIEKRRAYFRRVRFNSYFHWLEIGLQHLYNLSVPLNESTWELFDERIKG
                     AGRNPNHHLEILEDRCHYEQVLLDAYWAPGSDDGHPELFKPVYRINQFLFGYSSEAKD
                     HNGNNPFQNEHWPATINLDEYIVLMEHQIIKQKEAGCIALKSALAYDRGLDFSDPTKD
                     QAEKGFRNPNAGPEEIKAFQDYVFRQICRIAAEQNLPLQCHTGLGQLEKTNAMQMKPV
                     IEENPDTQFVLFHGGYPWIQDVCALVHNFPNVYADICWLPILSGPSCKSLVREMIEVG
                     TSDRMMWGCDTWTSEESYGAMIAAKRVLSAVFAEYVDDGYLSFTDAIEFMDYIFYRNP
                     KEVFRL"
     gene            3550..4113
                     /locus_tag="PK898_07920"
     CDS             3550..4113
                     /locus_tag="PK898_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HOU19996.1"
                     /translation="MESIFRPKWWVFLIRGIVVLLFGLMALTRPGMTLSALILTFGIF
                     AIADGIVETVIGIVSYKSNDLWWSVLLSGIASILIGILTLTNPHITGLVLLYLIGLRA
                     FVLGFLEVVAAIELRHIIEGEWMLIVGGILSIIFGVMLFIYPAEGALSLLWLIGIFAV
                     IGGVWLILFSFHMRSAVKKIDRFLEGE"
     gene            4459..5451
                     /locus_tag="PK898_07925"
     CDS             4459..5451
                     /locus_tag="PK898_07925"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU19997.1"
                     /translation="MKVLVTGGGGFLGLNIVKRLLYEGDEVVCLDQNELGAEALEFLN
                     DHSSAFFFEKGDVRDFEKIRSVLNRYDIRRVIHAAAVTPSLEEEKANPERIMEINFGG
                     TVNVIHAARESAVDRIVFVSSNSIYGDVEADDVRVTEEHPLYGRNLYSIAKIASEMYC
                     GRIQELSGLQILIGRVSSAYGPMEHPTSARNHLSLVYQIVQQAIVGNEMKIFGESASR
                     NWTHVYDLAAALTTLLKKEDLSYSVYNLSYGCSYSLKELLETVKKVEPKFRYSFTDTE
                     KDANLILSTGEHRGILDNSRLIHDAGFVPAYNLQKGLEQYFSWLRQAKLTNDFR"
     gene            5589..6821
                     /locus_tag="PK898_07930"
     CDS             5589..6821
                     /locus_tag="PK898_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU19998.1"
                     /translation="MPEAAAWKFQKVLKPIIDIHSGHYILKGRILTCVHGEVIENGFV
                     EINGKTIADVGKWDPSAEYADAEIIEVENGTIMPALFNNHAHLAWDGIHDLADQSLND
                     TPEISAYKSCANMLLSLRAGITTVRDLGVNKANIATKQAVEQGIFPGPRLLITGEAIV
                     QTGGHTYWACREATGADEMRRAVRDQVRNGADLIKIMACHDRLEFTDSELEAIIDEAH
                     RNGLPVTAHATFNAAVKRMAQFGVDVIEHGGDMDDETIGLLLEKKIPIVTTFSAVVIQ
                     SRPEIARKYNIPEWKIEERQRQAADKAKYNDLVKASKAGVMIAFGTDAGSPCVQHNMI
                     VPEMEFMVHLGLVDGNYGALRSATSVSAKLNKLDQKLGTLEKGKLADVIVMEGNPLED
                     LSALTKVKYTFKEGCSML"
     gene            6904..7995
                     /locus_tag="PK898_07935"
     CDS             6904..7995
                     /locus_tag="PK898_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU19999.1"
                     /translation="MIRIIQVGLGVRGIQWADVIRKHPDTTVVAYVRRNVELARKQVT
                     EWGETVPCFSTLEEALNSVRADAVVLVTPPEYHFEDVLCAFSHHCHVICEKPLSEKMC
                     ECIEMVRQAEQNKLLLMAGMNFRYLAVTQKYRNLINEKVLGTIGYGHFTYIRNRNGNR
                     NDLNKYPLSMLQPMLLEQSVHHFDLLRYCYGEEPCRVSAETWRPYWSTYDDNCCVSAS
                     IRFTHGIQAVYLGTWTSGCNRMQFEWRTDFSGGTIRQCHQFGDLFQSRMDPELSMSGE
                     NFKTDPSIEPWEPVELDPCEAFVDDTYGLLCEFVDALQAGQELVTSGKDHLKTLGLTL
                     ACIESSLTGRRIEMHDFYKRNGVPSRWID"
     gene            8030..9460
                     /locus_tag="PK898_07940"
     CDS             8030..9460
                     /locus_tag="PK898_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20000.1"
                     /translation="MTDRNEKFVGIQISPFSVMDEGIVPMLDRFENRFGINTLLVGTL
                     SWLGLKVGRSIGWQFDGWPDHGKADADYQLKGGSYIKERAEYYRNTKVTNFQNKDEGL
                     EGKDILDIVIPEARKHGMKVYPEVMEPLFKYAGHGSANNVSIPNMPQLLEVDQFGRYG
                     HEPCINNPGYRSFWHSVIEDYARNYDIDGIMWCNERRSPLDNILSGSPANCFCPYCRQ
                     EAFERGIDVEKVREAFGKLYDVTQRARNGEQFVDGALIEFMRTLYYYPEVMQWERYWV
                     ERNKDLDRELYGITKWCNSDLQFGLNVWNRNHFNPLRKAQWPWAEMVTWSDWVKPITY
                     QHQAGGVYMNEMKSLHQSVLRDVTPKEMTPIMYKFLGLNECDWDELYEKGMAPETYVY
                     GQCADAVRGVNGKVPVYMGIGVDAPRSGPNQAICTPDIVRRSVLAAYRAGGKGVVYSP
                     SYAGMKLKNLDGGAEALKELGIFSNR"
     gene            9518..>9906
                     /locus_tag="PK898_07945"
     CDS             9518..>9906
                     /locus_tag="PK898_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20001.1"
                     /translation="MMKKIMIDPTIRKEQVDAIQMQLPKDWEIIDQIEKADFIVTGNM
                     DISEDNLRSCGGSLKGIIQILPGSAKIAQVGIPVYSVYDTGLTGVAEHAIMLMLVLSR
                     RLLWLNEKVKNQEWVEGKSTPVLTDQK"
     assembly_gap    9907..10006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10007..10629
                     /locus_tag="PK898_07950"
     CDS             <10007..10629
                     /locus_tag="PK898_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU20002.1"
                     /translation="NQEWVEGKSTPVLTDQKNYTYNWVGLPESGQLHGKTVGIVGFGF
                     IGQDLARRLKAFHAKIIYYDIVRASSDIETLYEAKYAPLDELLQCSDFVTLHLRYIEG
                     EGGNKKMFGKQLFEKMKPTAYFINTSRGQLVDEAALAEAIRNNRIAGAGLDVFEYEPF
                     KPDCPLLPLISDHVILTPHVAGTYVEEARKTIAMEIVEIIREKSYR"
     gene            10626..12011
                     /locus_tag="PK898_07955"
     CDS             10626..12011
                     /locus_tag="PK898_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU20003.1"
                     /translation="MKRETPAELKQIFRWIDAHQDEFVKDLQRLVQQPSISAQNIGLR
                     ECASLVCEIMHEDGLPAQQFELNGGPPIVMGELQSKRNKKKLMAYAHYDVQPPEPLDK
                     WVFPPFGGEISDGRVWGRGATDNKSGLLAFVKAAEAWMNGIGDVPVTIKFMFEGEEEI
                     GSPHLGPFVAENPEMFRCDAMHCLDGGMNSSEPVPEIDLGLKSVLYVELVAHGANSDI
                     HSLNAPLVPQPAWDLVRALNTLTDEKRHILIEGWYEGWSEPDEDDIRLIKDKVSRMDI
                     DKLKKEWGLTEFALGRDGFEAMKARAFEPTCNICGLTAGYQGPGDKTIVPCEARAKID
                     FRLPPFIDPSKALEKLRRHLDKNGFEDIEVISVFGDSPEPPFKISVKEDVSRAVIEAA
                     EMVYGIPPIINGVSAEGTILRHVWMPTVLTGFAQPDCNLHAPNESCSISAYMDGIKYA
                     AAIMWNFGNMG"
     gene            12054..13481
                     /locus_tag="PK898_07960"
     CDS             12054..13481
                     /locus_tag="PK898_07960"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU20004.1"
                     /translation="MIIEDFVDYAVKTGANYADARIVNRSGEISSVWDGNIEEQIHNR
                     SYGYGIRILFHGAWGFAASNDFSKWKEVVLSAMKVAQASLKRQKEPVCLAPVPVIRDH
                     YRTPQKIDVRTVSLNERLDLLLEADHSMASVPGITQRKSTMGVYSTDILFASSEGSFI
                     EQNLVACGAGTVAYASNGSDTAFRSYPASFQGDYSSAGYEHILGLNLIEGSKRFAQQA
                     VELLSAKRCKSGKKTVILDSSMLTLMVHESIGHALESDRVLGSEASYAGTSFATPEKL
                     ADYHYGSEQVTITSDPTLPGGLASYGYDDEGYPACRTVLIDHGILVGYLTGRETAAKL
                     KLPLTAAMRSDGWQRFPLIRITNVNLEPGDSSLSKMISEVDDGFYMETVKTYSIDDKR
                     LNFQFAPEIAWEIKNGKIGSMVKNVRYSGMTPEFWNSCDAVANRDEWHLWGIPVCGKG
                     EPGQVVMVGHGTAPARFQNVSVLGD"
     gene            13486..14853
                     /locus_tag="PK898_07965"
     CDS             13486..14853
                     /locus_tag="PK898_07965"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU20005.1"
                     /translation="MRWDREQTYELLKRIIDLSEADQTEACFMAYESGLTRFAENRIH
                     QNMCEEDAIVVIRVAIGKKLGSVSANQFDEASLVCAIQKAIEIASLNQPNELFDGFAE
                     KQPVDSIRLPWYIPETASAGTNERADIASRLIQKVENSAYEAAGSVSNGVYVSSVLTS
                     LGQFVYQQETRCGGLTVVNRPGEAGFGTGYSEYFGRDIHLIHPEELAAKAVCISAMNH
                     DAKSLPPGDYPAVLTPASVGLLIFYLGWMALHARAVQSGQSFLAGYFGEEVLDPKLSI
                     WDDALDGRNYGMSFDWEGNPARYVDLIRNGKAIDVVYDTFTAKKVGKKSTGHALSPLR
                     DRYYSSPMAEHLIVGTGDTPLEKMIEKTEKGILINHLWYVREVNFKSASVTGMTRDGT
                     FMIENGKITHALKNLRFSISLRDVFQSIEMIGTEQELTPVSIGTVLAPAMKLKHFNID
                     GSSSY"
BASE COUNT         4242 a         2792 c         3605 g         4229 t
ORIGIN      
        1 tgatttttag tcaccggaaa aattctgatt ttaatgatcg cggctgtgga aacctggggc
       61 tcggatcaat ggaacaatct tcagaattta atgtcaaatt tggattataa tatggatcac
      121 cttcttctaa tttatcatgc catttctttt ttattatcat tttttcaaaa gtaaaacgtt
      181 caattttttc cggcgtatct tcatatccac gggtttttga ttcaaaatga taaagttgtg
      241 cgtatggcgt ccaaacattt ctgtaacctt tttcttgaat tttcaagcat aaatcgatgt
      301 caccaaatgc taactgaaaa gccggatcga atccgttgat ttcaataaaa atggactttt
      361 ttatcatcaa acaggcccca gttaccgcag aataattttg tggtactata aggcgaagga
      421 ttccttctga ttcaattttt ggttttcctt tatatgcatg tccggcgatc cctcccaatc
      481 ctacaattac accgccatgt tggatggtac catccggata aaataatttt gcccctaccg
      541 cgcctacatc cggacgaatg gcaaaggcta acatctgttc cagccaatct tctgtgatca
      601 cttcagtatc attgtttaag aataatagga tttctccttt tgctgaatga atagcgaaat
      661 tattaatttt ggaaaaattg aaaggatagt tatattttaa aatttttaca ttttcgtatg
      721 tttccaattt tttataaata tcaaatacgg tgtcttctgt ggagttattt tctatgatga
      781 ggatttcaat atttttataa gtcgaaccat taataataga atcgacacat tttgttaaat
      841 cttctccgtg gttatgattt ggaataataa ttgaaactaa agggcttttt tcaaagttat
      901 cgtaaagtat tttatatgaa gtggggaaag gtccttgaat aacttttcct ttaagtccta
      961 atttatcaat atgttcttga acggctttaa tcccggaagt cgttgcatag gattttgatt
     1021 ccagtgaaag cgctgtcgat ccttccatcg cacgccagtg ataaaggatt tgcgggatat
     1081 gggtaacaaa tcgcgctttt tctatcgctc gtaagacaag atcaaaatcc tgagcgcctt
     1141 caaagccatc tctcaaccat ccgatctgat ctcctagtga ttttctaaca actagcaaat
     1201 gacagatgta atttacagcc cgcagaaaat ccggactgta tgccggtttg aaaaacggac
     1261 cataccgaaa aattccatct tcattcaact tatcttcatc cgaataaaag acatctgtat
     1321 caggatattg atgaagtgca ttaatcaccg ccgaaagagc aaaaggggca agcaaatcat
     1381 cgtgatccag aaaagcgata aaatctcctt ccgcaatttt aatggctgca ttcgtgttgc
     1441 cggcaatccc caaattttta tctaaattta gaactttaat ccgtggatct tttttagaat
     1501 aattttccac aaggtttgct atttttgcat cttttggact tccgtttgct atgcataatt
     1561 cccattttgt gtaatcttga tttagaacag aatcaatcgt ttcagtcaat attttagtcg
     1621 gagttttata aaccggcatc acaacgctga ttaaagttgc tgaattttga tttaaattct
     1681 tctgcagagt tttcctatag agtggttcat ttttttggat ccattgatta taagaatatg
     1741 attcaaatga aatatttttt cgaaacaatt tgtataaatt atgataaatt aataaacaaa
     1801 aatgaaaaat cgatctgata attctatttc gaaaacttaa aggaggtgca actttactat
     1861 aaaatctttc tattttataa gctaatttcc aggattgggt tgaataaata tcctgaatta
     1921 tcttattctt ttccgaaatc tccttttcag ttaatgctat ctgcttgacc agttcagcta
     1981 taaaatcatt attatctttt gaattatcat tgggattggt attgttatac atgagaaaaa
     2041 attataacat atatttgaat caatatacta ttttatgtta aacaaattat attataaaaa
     2101 taaaatgact tcatctcgat tatttgaata attccgttcc cctacaatcg aaacacttct
     2161 ttcgggttcc gataaaaaat ataatccata aattcgattg catccgtaaa ggacaaatat
     2221 ccgtcgtcca catattccgc gaaaacagcg gaaagaaccc gttttgcggc gatcatcgct
     2281 ccgtagcttt cctcgctcgt ccaagtgtcg caaccccaca tcatccgatc cgacgtgccg
     2341 acctcgatca tctcccgaac caagctcttg caagacgggc cggataggat cggcagccag
     2401 cagatgtcag catagacatt ggggaaatta tgaaccagcg cacatacatc ctgaatccaa
     2461 ggataaccgc cgtggaacag cacaaattgt gtatccggat tttcctcgat gaccggtttc
     2521 atttgcatgg cgtttgtttt ttcgagctgt cccaaaccgg tatggcattg gaggggcaga
     2581 ttttgttccg cggcgatccg gcaaatttgg cgaaaaacat aatcctgaaa agctttgatt
     2641 tcttccggac cggcattcgg atttcgaaag cctttttctg cttgatcttt ggtcgggtcg
     2701 gaaaaatcca gcccgcgatc atacgctaaa gcggatttca aagcgatgca tccggcttct
     2761 ttttgtttga tgatttgatg ctccatgaga acgatatatt catctaagtt aattgttgcc
     2821 ggccagtgtt cattttggaa cgggttgttc ccgttatgat ctttcgcttc cgatgaatat
     2881 ccgaacagaa actgattgat gcggtagacc ggtttgaaca attccgggtg cccgtcatcg
     2941 gagccgggtg cccaataagc atccagcagg acctgctcat agtggcatcg atcctccaaa
     3001 atttcaagat gatggttcgg attccggccg gcgcctttga ttcgttcatc gaataattcc
     3061 caggtggatt cgttcaacgg tacggaaaga ttatataaat gctgcagacc gatctcaagc
     3121 caatgaaaat aagaattgaa acgcactcgt cgaaagtatg cccggcgttt ttcgatggtt
     3181 tcatcaaaat cgaccccaca ccatgaaaca taagcgttcc gcagcagcga ctccaggttc
     3241 aactcatttt gaaaagagtc atccagatga tggcaatggg tgttgatgat ccgttgtcca
     3301 tccaaagccg ttaatagttc ccgagcggtt tccgacaaag caattccttc cttctttgaa
     3361 tctgatccaa ttctacttca tcttgccgga aaaaagctca tcaaatccgg aagaatttcg
     3421 tgtgattatc tgcgaaacga gagatttccg ctcgtctccg ccgataagag gattataatt
     3481 tgatgatggc agacggagtc gatccgcctg ctcaaatctg tgccatttca aggagaaagg
     3541 aactgaatta tggaaagcat ttttaggcca aaatggtggg tgtttttgat tcgtggaatt
     3601 gtcgttttgt tatttggtct tatggctctg acacggccgg gaatgacact tagtgcattg
     3661 attttgacgt tcgggatatt tgcgattgcc gacgggatcg tggagactgt aatcgggatt
     3721 gtttcctata aatccaatga tctttggtgg tcggttttgt taagcgggat tgcaagtatt
     3781 ttgatcggta ttttaacgct caccaatccg catattacgg ggctggtatt gctatatctg
     3841 atcggattgc gtgcttttgt actcggcttt ttggaagtgg tggccgcaat cgagctgcgc
     3901 catataatcg aaggcgaatg gatgctgatc gtcggcggga ttttatcgat catcttcggc
     3961 gttatgttgt ttatctatcc ggcggaaggt gcgctcagtc tgctgtggtt gatcggcatt
     4021 tttgcggtga tcggaggagt gtggttgatt ttgttctcat tccatatgag aagcgcagtc
     4081 aagaagatcg atcgttttct ggaaggggaa taacaaaagt tcgacggata ccgctaaccg
     4141 attaaaagcg gtaaataaaa aagatgcaaa cccatttcaa cggtttgtat ctttttttgt
     4201 tacgggagcg atgagttcaa ctcgcaattt cggtcgatta aagcggagtt tcctcaatgg
     4261 ttttcgataa ggaacaaccg cttcaataat tctctttttt tcgacgaatt ccgcaattat
     4321 tacgaacaat aaaaatcggg cgattttgct tgctatatta atagaaataa agttgccaaa
     4381 atatccaaaa atatgataaa aatcataaaa tcttgcttat aaacattttg tttcaattct
     4441 taaatagaag aggaaattat gaaggttttg gttaccggcg ggggtggatt tctcggttta
     4501 aatattgtaa aacgacttct ctatgaaggg gatgaggtcg tttgtttgga tcaaaacgaa
     4561 cttggggcgg aagcacttga atttttgaat gatcactctt ccgctttctt ttttgaaaaa
     4621 ggggatgtca gagattttga gaagatccgg tcggtattga accggtatga cattcgccga
     4681 gtgattcacg ctgctgcagt cacaccctct cttgaagagg aaaaggctaa tcccgaacga
     4741 atcatggaaa tcaattttgg cggaacagtc aatgtgattc atgcggcaag ggaaagtgct
     4801 gttgatcgga tagtctttgt gagctcgaac agtatctacg gtgatgtgga agcggatgat
     4861 gtgcgagtta cggaagagca tccgttgtat gggcggaatc tttacagtat cgcaaaaatt
     4921 gcaagcgaaa tgtattgcgg acgcatccaa gaattgtccg gattacagat tttgatcggc
     4981 agagtgagtt ccgcttacgg accgatggaa cacccgactt ctgctcgtaa tcacctgtca
     5041 ttggtttatc agatcgttca acaagcaatc gtaggaaatg aaatgaaaat ctttggcgaa
     5101 agcgcaagcc gtaattggac acatgtttat gatctggccg cagctcttac gacattactc
     5161 aagaaagaag atctatctta ttccgtgtac aacttatcct atggatgttc ctattcgctg
     5221 aaagaattat tggaaaccgt aaaaaaagtg gaaccgaaat ttcgatattc ctttacggat
     5281 acggaaaaag atgcaaattt gattctctca accggggaac acagaggaat tttagacaat
     5341 tctcgtctga tccatgatgc gggctttgtc cctgcctaca atttacaaaa gggtttggaa
     5401 caatattttt cttggctgag acaagccaag ttgacaaatg attttcgatg aaacagcaca
     5461 ataagaaaat aaatttctta atttgttgcg aaaactaaaa aaatgatata atattttctc
     5521 aagcaaaaaa agcttggtgg ttccgcattt tttaatattt caaactaaat aattctatcg
     5581 gaggcaaaat gcctgaagca gctgcatgga aatttcaaaa ggtattgaaa ccaattattg
     5641 acatccattc gggacactat attctaaaag gaagaatact gacttgcgtt catggtgaag
     5701 tgattgagaa tggttttgtt gagatcaacg gaaaaacgat cgctgatgtg gggaaatggg
     5761 atccgtccgc ggaatatgcg gatgccgaaa tcattgaggt cgaaaacggt acgattatgc
     5821 ccgcattatt caataaccat gcccacttgg cgtgggatgg gatccatgat cttgccgatc
     5881 aatcattaaa cgacactccg gagatcagtg catataaatc atgtgccaat atgcttctgt
     5941 ccttacgagc cggaattaca acggttcgtg atctcggtgt gaacaaagca aatattgcga
     6001 cgaaacaagc tgtcgaacag gggattttcc cgggtccgcg tttgttgatt accggtgagg
     6061 caattgttca gaccggcggg catacttatt gggcatgtcg ggaagcgacc ggtgcggatg
     6121 aaatgcggcg ggcggttcgt gatcaggttc gaaacggtgc cgatttgatc aaaattatgg
     6181 catgccacga tcgactggag tttacggatt ccgaattgga agcaattatt gacgaagcgc
     6241 atcggaacgg tcttccggtg accgcccatg ccacctttaa tgcggctgtg aagcgaatgg
     6301 cgcaattcgg tgtggatgtt atcgaacatg gcggggatat ggatgatgaa acgatcgggt
     6361 tgctgttgga gaaaaagatc ccgattgtga ctactttctc ggcagtcgtg atccaatctc
     6421 gcccggaaat tgcgcggaaa tataatattc cggaatggaa gatcgaagaa cggcaacggc
     6481 aggctgcgga taaggcaaaa tataatgacc ttgtcaaagc gtccaaagcc ggagttatga
     6541 tagcattcgg aacagatgcc ggaagtccgt gtgtgcagca taatatgatt gtcccagaaa
     6601 tggagtttat ggttcatttg ggtttggtgg atggaaatta cggcgcttta cgaagtgcga
     6661 caagtgtttc ggcaaagtta aacaaactgg atcaaaagtt gggaacgctt gaaaaaggga
     6721 agttggcgga tgtcatcgtg atggaaggga atcctttgga agatctttcc gcattaacga
     6781 aagtgaaata tacctttaaa gaaggctgtt ccatgcttta ggaagacttt ttttgtacgg
     6841 ggaccgataa cggttgaatt cggctgcggt gttaatggtt tttcgttttg agcgggggtg
     6901 atgatgatca gaatcattca agtgggtttg ggtgtgagag gcattcaatg ggcggatgtt
     6961 atccgaaagc atccggatac aactgttgtg gcttatgttc gccggaatgt tgagttggcg
     7021 cgcaaacagg ttaccgaatg gggtgagacg gttccttgct tttcaacttt ggaagaagcg
     7081 ttgaattcag tccgggcgga tgcggtggtt ttggtgactc ctcccgaata tcactttgag
     7141 gatgtcttat gcgctttttc tcatcattgc cacgtgatct gcgaaaagcc cctttctgaa
     7201 aaaatgtgcg aatgtattga gatggtcagg caagccgagc aaaataaatt gcttttgatg
     7261 gccggaatga attttcgcta tttggcagtg actcagaaat atcgaaatct gatcaatgaa
     7321 aaggttttgg gaacgatcgg gtacgggcat tttacttata ttcggaaccg aaacggaaat
     7381 cgaaacgatc tgaacaaata ccctctatca atgctgcaac cgatgttgct cgaacagtcc
     7441 gttcatcatt ttgatttgct gcgttattgt tatggggaag agccatgccg agtttcggcg
     7501 gaaacttggc ggccttattg gagcacttat gatgataact gttgcgtttc cgcctcgatc
     7561 cgttttacgc acgggattca ggcggtatat ttgggaacct ggacatcggg gtgtaaccgg
     7621 atgcaattcg aatggcggac agatttttcg ggaggaacca tccggcagtg tcaccaattc
     7681 ggtgatttgt ttcaatcgag aatggatccg gaattatcga tgagcggaga gaattttaaa
     7741 acagatccga gtatcgaacc gtgggaaccg gttgaattgg atccgtgcga agcatttgtg
     7801 gatgatactt acgggttgtt atgtgaattt gtcgatgcgc ttcaagcggg tcaggagttg
     7861 gtaacttccg ggaaagatca tcttaagaca ctcgggttga cattggcgtg tatcgagtct
     7921 tctcttaccg gcagaagaat cgaaatgcat gatttttata aaagaaacgg cgttccttca
     7981 cgatggatcg attaaatatt tttttgataa cggaatttag gagaaaataa tgacggatcg
     8041 gaatgaaaaa tttgtgggta ttcaaatcag ccccttcagt gtgatggatg aaggcatcgt
     8101 ccctatgtta gatcgtttcg aaaatcggtt tggtatcaat actttactgg tcggaacatt
     8161 atcatggttg ggattaaagg tcggtcgcag tatcggctgg caatttgacg gttggcctga
     8221 tcacggcaaa gcggatgccg attatcagct gaagggcggt tcatatatta aagaaagagc
     8281 cgaatattat cgaaatacga aagtcacaaa ttttcaaaac aaagatgaag ggctggaagg
     8341 aaaggatatt ttggatatcg ttattcccga agcgagaaaa cacggaatga aagtgtatcc
     8401 cgaagtgatg gaaccgctgt ttaaatatgc gggacacggt tcggcgaata atgtttccat
     8461 tccgaatatg ccgcaactgt tggaagtgga tcaatttggt cgctacggtc atgaaccctg
     8521 tatcaataat cccggttacc gcagtttttg gcattccgta attgaagatt atgcgcggaa
     8581 ttatgatatc gacggaatta tgtggtgtaa tgagcgaaga agccctctgg ataatattct
     8641 gtccggctct cccgcaaatt gtttttgtcc gtattgccgg caagaagcgt ttgaacgcgg
     8701 aatcgatgtc gagaaagttc gggaagcatt cggaaagttg tatgatgtta cacagcgagc
     8761 cagaaacggt gaacaatttg ttgacggagc gctgattgaa tttatgcgga cactctatta
     8821 ttatccggaa gtgatgcaat gggaacggta ttgggtcgag cggaacaaag atttggatcg
     8881 tgagttgtat ggcatcacaa aatggtgtaa cagcgatttg caattcggat tgaatgtctg
     8941 gaatcggaat cattttaatc ctttgcgaaa agctcaatgg ccgtgggctg aaatggttac
     9001 gtggtccgat tgggtaaaac cgattactta tcaacatcag gccggcggcg tttatatgaa
     9061 tgaaatgaaa agtctgcatc aatccgtttt gcgagatgtg actccgaagg aaatgacgcc
     9121 gattatgtat aaattcctcg gattgaacga atgcgattgg gacgaattat atgaaaaagg
     9181 gatggcaccg gagacttacg tttatgggca atgtgccgat gcggtccgag gtgtcaacgg
     9241 aaaagttccg gtttatatgg ggatcggcgt ggatgcgccc cgcagcggac ccaaccaagc
     9301 aatctgtaca cccgatatcg ttcggcgcag tgttttggct gcgtatcgtg ccggcggtaa
     9361 gggcgtcgtt tattctcctt cttatgccgg aatgaagttg aaaaatctgg atggcggagc
     9421 ggaagcgttg aaagagctgg ggattttttc aaaccgataa tagcagttga aagcctttga
     9481 aacagaaata ttcttaaatt atgaaagtgc tgggaaaatg atgaaaaaaa taatgatcga
     9541 cccgaccatt cgcaaagagc aagtcgatgc gattcagatg cagctcccaa aggattggga
     9601 aatcatcgat caaatcgaaa aagcggattt tatcgttacc ggtaatatgg atatttccga
     9661 agataaccta cgatcttgcg gaggatccct aaagggaatt attcaaattc ttcccggcag
     9721 cgcaaaaatt gcacaagtgg gaattcctgt ttattcggta tatgataccg ggctgaccgg
     9781 cgtagcggaa catgcaatca tgttaatgct tgtattaagc cggcgtttgc tgtggttgaa
     9841 tgaaaaagtg aagaatcagg aatgggtgga aggaaaatcc acgccggtac tgacggatca
     9901 aaaaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagaa tcaggaatgg
    10021 gtggaaggaa aatccacgcc ggtactgacg gatcaaaaaa actatacata caattgggtc
    10081 gggttacccg aatccggaca actacacggt aaaacggtgg gcattgtcgg attcggtttc
    10141 attggacaag atttagctcg ccggttaaaa gcttttcatg caaaaattat ttattatgac
    10201 atcgtcaggg cttcgagtga tatcgaaact ttgtacgagg caaagtatgc tccgctggat
    10261 gagctattgc aatgttccga ttttgttacc cttcatctgc gatatatcga aggagaaggg
    10321 ggaaataaaa aaatgttcgg gaaacaactt tttgaaaaaa tgaaaccgac cgcttatttt
    10381 atcaatacct ccagaggtca gttggtagat gaggctgcgt tggcggaggc aattcggaac
    10441 aaccggattg ccggtgccgg tctggatgta tttgagtatg aacctttcaa accggattgc
    10501 ccgctccttc ctttgatcag cgatcatgtg attcttaccc ctcatgtcgc cggaacttat
    10561 gtggaagaag cccggaaaac aatcgctatg gaaatcgttg aaattattcg ggagaaaagt
    10621 taccgatgaa acgagaaacg cctgccgaat taaagcaaat ctttaggtgg atcgatgccc
    10681 atcaagatga atttgtgaaa gacctgcaac gtttggtgca gcaacccagc atttccgctc
    10741 agaatatcgg tttgcgtgaa tgcgcttcct tggtgtgtga aataatgcat gaagacggat
    10801 tgccggcaca gcagtttgaa cttaacggag gtccgccgat tgtgatggga gagctgcaaa
    10861 gcaaaaggaa taaaaaaaag ctgatggctt atgctcatta tgatgtccaa ccgccggaac
    10921 cgttggataa atgggttttt cctccgttcg ggggcgaaat aagcgatggc cgagtttggg
    10981 gacgaggtgc gaccgacaat aaaagcggct tactggcatt tgtgaaagcg gctgaagcat
    11041 ggatgaacgg cataggtgat gtaccggtta cgatcaaatt tatgtttgaa ggagaagaag
    11101 aaatcggttc tccccatctg ggaccattcg ttgcggaaaa ccccgagatg ttccggtgcg
    11161 atgcgatgca ttgtctggac ggcgggatga attccagtga gcctgttccg gaaattgatt
    11221 tgggcttgaa aagtgtttta tatgtggaat tggttgctca cggtgcgaat tcggatattc
    11281 acagcttaaa tgctcctttg gttcctcaac cggcttggga tttggtgcga gctttaaata
    11341 ccttgacgga tgaaaaaaga catatattaa tcgaaggttg gtatgaaggt tggtcggaac
    11401 cggacgaaga cgatattcgg ctgatcaaag acaaagtcag tcgtatggat atcgataaat
    11461 tgaaaaaaga atggggactg accgaatttg cgttagggcg tgatgggttc gaagcgatga
    11521 aggcgcgagc ttttgaaccg acttgtaaca tatgcggtct gacagcagga taccaaggac
    11581 cgggagataa aacaatcgtt ccctgtgaag caagagcaaa aattgatttt cgcctgcctc
    11641 cgtttatcga tccttccaaa gcgttggaaa aattgcgccg gcatctggat aagaacggct
    11701 ttgaagatat tgaagttatc tcggtttttg gcgattctcc cgaaccaccg ttcaagatca
    11761 gcgtcaaaga ggatgttagc cgggctgtaa ttgaagcggc agagatggtt tatggaattc
    11821 cacccataat aaacggtgta agtgcggagg ggaccatttt aagacatgtt tggatgccga
    11881 cggttttgac cggttttgca caacccgatt gtaatttgca tgctccgaat gaaagctgct
    11941 ccatttcagc ctatatggac ggaattaaat atgctgccgc tatcatgtgg aatttcggaa
    12001 atatggggtg agccattatt tccccaaaaa agtaccggag gcttttcaga taaatgataa
    12061 ttgaagattt cgttgattat gccgtgaaaa cgggagcaaa ctatgccgat gccagaatcg
    12121 tgaatcgatc cggtgagata tcaagcgttt gggacggtaa tatcgaagag cagattcata
    12181 acagaagtta tggatacggt atacgtattt tatttcacgg tgcatggggt tttgcggctt
    12241 ccaatgattt ttccaaatgg aaagaagttg ttctttcggc aatgaaagtg gcgcaggcaa
    12301 gtttaaagag acagaaagaa ccggtttgtt tggctccggt tccggtcatt cgcgatcatt
    12361 accggactcc tcaaaaaatt gatgtccgta ccgtatcgtt gaatgaacgc ttagatttgt
    12421 tgctggaagc ggatcattcc atggcttctg ttcccggaat tacacagaga aaatcaacca
    12481 tgggagttta ttcaacggat attctttttg cttcaagcga aggatccttc atcgaacaaa
    12541 atcttgtcgc ttgcggagcc ggtaccgtag cttatgcatc aaacggcagt gacaccgctt
    12601 tccggagcta tccggcatct tttcagggag attacagcag tgcgggatat gaacatattt
    12661 taggcttgaa tttaatcgaa ggttcgaaaa gatttgccca acaagcggtc gaacttttat
    12721 cggcgaaacg atgtaaatcg gggaaaaaga ccgttattct ggacagttct atgctgacat
    12781 taatggtaca tgagagcatc ggtcacgcat tggaatcgga tcgtgtctta ggcagtgagg
    12841 ccagttatgc cggaacaagt tttgctacac ccgaaaaact ggctgattat cattacggat
    12901 cggaacaggt aacaatcact tccgacccga cactcccggg cggacttgcg agttatggat
    12961 atgatgacga aggttatccg gcatgccgga cggtattgat cgatcacgga atattggtcg
    13021 gttatcttac cggtcgggag accgcggcaa aattaaaatt accccttacc gctgcgatgc
    13081 gctcagacgg ttggcaaagg tttccgctca ttcgcatcac aaatgtcaat ttagaaccgg
    13141 gggattcttc attgtcgaaa atgatttcgg aagtcgatga cggtttctac atggaaaccg
    13201 taaaaacgta cagcattgat gataaacggc taaattttca atttgcgcct gaaattgcct
    13261 gggaaattaa aaacgggaaa atcggttcga tggtgaaaaa cgtacgatat agcggaatga
    13321 caccggaatt ttggaattcc tgtgacgcgg ttgcaaatcg agatgaatgg catttatggg
    13381 gaattccggt ttgcgggaaa ggtgagccgg gtcaggttgt gatggttggg cacggaacag
    13441 ctccggctcg atttcaaaat gtgtctgttt tgggagattg aacgcatgcg atgggataga
    13501 gaacagactt atgaattgct caagagaata atcgatttat ccgaagccga tcaaaccgaa
    13561 gcctgtttca tggcatatga atccggattg accagatttg cggaaaacag aattcatcag
    13621 aatatgtgtg aagaagacgc gattgtggtt attcgggttg ccattgggaa aaaactcggt
    13681 tcagtatccg caaaccaatt tgatgaagca tctctcgttt gtgccattca aaaggcaatt
    13741 gaaattgcat cgttgaatca gcccaatgaa ttatttgacg gttttgcaga aaaacaaccg
    13801 gtcgatagta ttcgtttgcc ctggtatatt cccgaaacag cttcggcagg aacgaatgaa
    13861 cgggcggata ttgcttctcg gttaattcaa aaggtcgaaa attcggcata tgaggctgca
    13921 ggatcggtgt ccaacggtgt ctatgtttca tcggttttaa caagtctcgg ccaatttgtc
    13981 tatcaacaag aaacacgctg cggggggttg acagtggtta acaggccggg agaagcgggg
    14041 ttcggaaccg gatattctga atattttgga cgcgatattc atctcatcca ccccgaagaa
    14101 ttggcggcaa aagctgtttg tatttcggca atgaatcatg atgcaaaaag tttgcccccg
    14161 ggagattatc cggctgtatt gactcccgcc tcggtcggtc tgttgatttt ttatctgggg
    14221 tggatggctt tgcatgcacg ggcggttcaa tccggtcaat cttttctcgc ggggtatttt
    14281 ggcgaagagg tattagaccc taaactatcc atttgggacg atgctttaga cggacgaaat
    14341 tacggaatga gttttgattg ggaagggaat ccggcgcggt atgttgatct gattcggaac
    14401 ggaaaagcaa tcgatgtcgt ctatgatact tttacagcga aaaaagtcgg gaaaaaatca
    14461 accggacatg ccttaagtcc tttaagagat cgttattatt caagcccgat ggcagaacat
    14521 ttaatcgtcg ggaccggaga taccccttta gaaaaaatga ttgaaaagac agaaaaagga
    14581 atattgatca accatttatg gtatgtgcgt gaggtgaatt ttaaaagtgc atcggtaacg
    14641 ggaatgacaa gagacggaac ttttatgatc gaaaacggca aaatcactca tgcgttaaaa
    14701 aatttacgtt tttcgatcag tctgagagat gtatttcaat caatcgaaat gattgggact
    14761 gaacaagaat tgaccccggt atccataggg accgtcctcg ctccggcaat gaaattaaag
    14821 cactttaata ttgacggttc ctcctcgtat tgatcgagga aaatgagttg taattatttt
    14881 taaaagagag attgattttt atctgaaaaa gacaaaatgt cggtataata agaaaaataa
    14941 atttcctttt tgtcgtttgt tgaaacaa
//
LOCUS       DAOSMM010000055        14747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4097_length_14747_cov_17.194868, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000055 DAOSMM010000000
VERSION     DAOSMM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 14747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14747
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4097_length_14747_cov_17.194868"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PK898_07970"
     CDS             complement(<1..745)
                     /locus_tag="PK898_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOU20006.1"
                     /translation="MASVKMLSYIKRDSPIHRLCGVTKLYTFLVWTIVCMLSYDTRVL
                     LVVMALSIIIFYLAKIKLEEVRFVLLFILFFLLLNNITIYLFSPEEGVKIYGTRHVLF
                     QFTNRYTVTKEQLFYLFNVTLKYFTVVPIALLFIVTTDPSEFASSLSRIGISYRISYA
                     VSITLRYIADIQSDYHNISQSQQARGVDLSKNVPLRKRIAGISSIAVPLIFSSLDRIE
                     LISNAMDLRGFGKKEKRTWYCARPFQRIDT"
     gene            complement(733..2448)
                     /locus_tag="PK898_07975"
     CDS             complement(733..2448)
                     /locus_tag="PK898_07975"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20007.1"
                     /translation="MSEPVIEFKNYSFHYRAQEKPTLYNIDLRITKGEKILIAGPSGS
                     GKSTIVHCINGLIPHYYKGESTGELLIHGEKAENLKIFDRSKIIGTVLQDSDGQFVGQ
                     SVGEDIAFALENECTPQPEMKDIVDRVSKIVNINHHLENAPQEISGGQKQRVSLAGVL
                     VDDVDILLFDEPLANLDPATGRYTMELIDRLSKETGKTIIIVEHRLEDVLWCPIDRVI
                     VMNDGRIVADEPPDQILSTGLLRQYGIREPLYITSLSYAGIQITPEMHPSHINTLKMD
                     AVRQPLLRWYQQIPPSEAESQAESILSLQNVSFSYDGLHEVLHHIRFDIHKGEMSAIV
                     GKNGAGKSTLCKLICGFEKPCSGDIIMNGKNLNGVSIRDRANTVGYVMQNPNQMISKV
                     LIREEVGLGLKLRDVDPQEIEERVNAVLQICGLYPFRNWPISALSYGQKKRVTIASIL
                     VLEPQIILLDEPTAGQDFRHYTEIMEFLQTLQERGLTILLITHDMHLMLEYTRRAIVI
                     ADGRLICDDSPARVLSDLNIIRPADLKETSLFTLAEKACLDDPLNFVQCFIDYDRRVR
                     KTWHQ"
     gene            complement(2537..3082)
                     /locus_tag="PK898_07980"
     CDS             complement(2537..3082)
                     /locus_tag="PK898_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HOU20008.1"
                     /translation="MKLTTRTIVAIGIGAAVFLVLNRFVAIPSGIPNTTINTAYAFLA
                     FMAVIFGPIAGALIGFIGHALTDAISYGSVWWSWVIVSALVGFAIGLCAKKINIEDGK
                     FEKKEILTFNIYQIVANLIGWGVIAPVLDILIYAEPSDKVFTQGIVAGIANIVTVAVL
                     GTAFLAIYARSRTKPGSLKQE"
     gene            complement(3207..3998)
                     /locus_tag="PK898_07985"
     CDS             complement(3207..3998)
                     /locus_tag="PK898_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20009.1"
                     /translation="MEKTILNKEKRSHVFTIDRIRYENEMTSSFIMKESLEGEPGQFL
                     LLWLPGVGEKPFSIAGTHPLVVTVADVGKLTHEIHALKPGDRVWIRGPFGQGYQAYGT
                     DALLVGGGYGASPLLFLAQALIEKGIRVQVFLGARTKDLLILKDQFEELPLKMYITTN
                     DGSCGIQGFVTVPLEESIRNLPDPQDACVYACGPKGMLMGIDALCEKYNIARQLSWEA
                     IMRCGMGLCGNCKIEMPDTWDEPSDKPGSKKAWLVCRDGPTSFTK"
     gene            complement(4003..4896)
                     /locus_tag="PK898_07990"
     CDS             complement(4003..4896)
                     /locus_tag="PK898_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20010.1"
                     /translation="MSNFSCKLFDNLILKSPLILASGISGNSDGLLVRAAKSGAAAVT
                     TKGVTLKYQKGAENPVSTDWGPGLITNLGLSNPGIESMAYMIKTFRNEMGDQTIPIFV
                     NIHGESAEEYAEIARLIAPSHPDLLEIDLPFPDETLAQEITHAVIENASGIPISVKIS
                     ADYPKIGQLAKVIEDAGADAISAINSMKGLIVDAYAAKPFLSSGVGGISGPAIKPIAL
                     YCISEIYQAVNIPVIGTGGVLTGLDMAEMIMAGATIVGIGSALYYSGYGAFSQIMNEF
                     ETFMKSEGYQSASELVGKIWR"
     gene            complement(5123..5872)
                     /locus_tag="PK898_07995"
     CDS             complement(5123..5872)
                     /locus_tag="PK898_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20011.1"
                     /translation="MYFEIKDLHVHYRAIHALKGISLTVQKGEIVALIGGNGAGKSST
                     LNSISGLVQTSGGSIIYQDEELIGLGTDIIVQKGIVQVPEGRHIFPKMTVQENLEMGA
                     FLNNNKASVNRDMENIFQLFPRLKERRNQPGGTLSGGEQQMLAIARGLMGHPDLMLLD
                     EPSMGLSPILVEQIFDIIFDINQRGTSILLVEQNAQMALAIADRGYVLETGEIVLNGT
                     GEELMNDPKVQAAYLGGESEEEKRNKGEYYF"
     gene            complement(5886..6719)
                     /locus_tag="PK898_08000"
     CDS             complement(5886..6719)
                     /locus_tag="PK898_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20012.1"
                     /translation="MTRKPPYVLEAQNITITFGGLTAVNNVSLQVPYQSIISIIGPNG
                     AGKTTFFNCISGFYSINSGVVLFQERPINGIPTDEIADMGISRTYQNIRLFSNMTVME
                     NVMIGQQPRLHANCLDAVLQTYRNRREEKQSIQKASNLLEFVGLSAYADDLAKNLPYG
                     AQRRLEIARALGNNPHLLLLDEPCAGMNPHETKAMIELINTLRSELKITIVLIEHDMK
                     VVMNISDSILVLDHGERIAEGSPEEIQKDQRVIEAYLGSGTKNVIEKYSKRKSEKVHQ
                     S"
     gene            complement(6716..8296)
                     /locus_tag="PK898_08005"
     CDS             complement(6716..8296)
                     /locus_tag="PK898_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20013.1"
                     /translation="MMNKLLSKAVKNGLVAGVILIFFEMINFTSTIAVILEKMFVSDK
                     KVKEVNPTYMIVIIGLILFLSTASFVRKNHLANEKSAVIKVLVSGLVSGLVSGILVST
                     LNAILGNYLNNGVDLRKYLDALSPDVIRNFLFQTDINNAIRIYMATTVGGAVLGAFAS
                     IVIQKASKLNGVFRSLKKGYNNLQIQKKCEKHPIIKKLFIVFVVIFIFALPMIINAYT
                     ISVLGIVFIYAILGLGLNLIVGLSGQLVFGYAAFYAIGAYTFALLTAPKPLGIEMSFI
                     PAFIIALISSGIGGLLVGLPVMNLRGDYLAIVTLGFAEIIRILINSNILADYTGGPQG
                     VKDIGQPHQPAFLVKILGKPLEENVWFLYLIIISLLVVIYIVYRLQYSRIGRSWEAMR
                     EDETVAQACGVTTSFYKVLAIVIGAIIAGAAGAIYVSRNTYTGPNEYAFMVSVNALAV
                     VVIGGMGSIPGALLGAFIIKGLPELLRDLDNYRMLVFGALLIIMMVIRPEGLLPAKRR
                     KLAVKPQSLEVLKEDLKP"
     gene            complement(8293..9396)
                     /locus_tag="PK898_08010"
     CDS             complement(8293..9396)
                     /locus_tag="PK898_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU20014.1"
                     /translation="MDADKSTPTTIQKTKVNRINWLRLIVGICAGVFLVYRLIIQIIR
                     PEYGPVYWLGFINTGLIVGGMYALIAIGYTLVYGVMMLINFAHGDIMMLGCFAGYFTF
                     EYLDFLTIGDSNFLNVYPILSIILAFLMGTGVATVSGYFLEKIAYRPLRNSHVRLAPL
                     ISAVGASIFLETAAQIMFGPNKKTYKNPEILSRGSGLNINMEDGMIIITKTGIFSLVL
                     AILLMIALYFFVQKTKLGKSIRAVSQDKATAQLMGINLDRVTSQTFLISGFLAGAAGV
                     MWGLHNGIFNHYVGFLPGIKAFTAAVLGGIGNIPGAMAGGMILGIVESVGPAFLGIDF
                     QLKDVISFTILILVLILKPSGLFGKSASNVEKV"
     gene            complement(9676..11181)
                     /locus_tag="PK898_08015"
     CDS             complement(9676..11181)
                     /locus_tag="PK898_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU20015.1"
                     /translation="MKKSFLFVVSILLISTLILTGCGSKATETPKPATEVVEEATTAP
                     SATTAPSATTEPSATTSSQAAAAVEAVENIVSTAANSAETAVESAEKTVGEAAATAVD
                     TAKTAVESVEKSGSEAGASDVMKAKGVADKSRCDAEGGCAVIKPGDTIKIGMGGPLTG
                     EYAMYGVDISDSEKISISDLKPLEGFEWELLAEDTVGSPETAVSIANKWVTDDTFVAV
                     AGNVLTGESAAIIPIMEKYYIPMLSPSATGANLTENNKVFNRIVFQDAEQGKFAAEFI
                     VNTLGYKKVAVLHDGMDYGKGIADTVRDTLVKLGVEPVAYEAITPGEADYSAVLTSIA
                     SKKPEIIYYGGYTAELSVIANQLGQVGLAGTPVFSDDGAFGAEFIEKAGKNAEGCYGT
                     SSVPADSEAKKAFDKKYKETLGREPGSKSTFPWFAYDVIQVLAKQISEVSFVGDDGNL
                     YVPRADLVKAVRSTSGFQGITGEITCSENGECNASGPTFYVVKDGKWVVAK"
     gene            11666..12085
                     /gene="rpsL"
                     /locus_tag="PK898_08020"
     CDS             11666..12085
                     /gene="rpsL"
                     /locus_tag="PK898_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOU20016.1"
                     /translation="MPTINQLIRKGRKTRSVKSKAPALQYTYNSSNQRRTKVAEGAPQ
                     KRGVCTNVRATTPKKPNSALRKIARVRLSNGFEVTAYIPGEGHSLQEHSVVLVRGGRV
                     RDLPGVRYHIVRGTLDTTGVAKRGQGRSKYGVKRAKK"
     gene            12101..12568
                     /gene="rpsG"
                     /locus_tag="PK898_08025"
     CDS             12101..12568
                     /gene="rpsG"
                     /locus_tag="PK898_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOU20017.1"
                     /translation="MRRTKPEKREVTPDVRYNSAVVTNLINRIMHNGKRSNAVRMVYS
                     AMDIVSQKTGKDGLEVLETALRNVGPVMEVRPRRVGGATYQVPMEVSANRRQTLAMRW
                     IIDASRSRTGKAFDECLAGELLDAYNNTGSAIRKREETHKMAEANRAFSHYRV"
     gene            12592..14664
                     /gene="fusA"
                     /locus_tag="PK898_08030"
     CDS             12592..14664
                     /gene="fusA"
                     /locus_tag="PK898_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU20018.1"
                     /translation="MAREYPLEKYRNIGVIAHIDAGKTTTTERILFYTGKTYRVGSVD
                     EGTTVTDWMDQERERGITIVSAAVTAEWKGYRINLIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDATQGVEPQSETVWRQADKYNVPRICFINKMDRTGASYHDSVESIRNRLGANPV
                     EMQIPIGSEGNFSGVIDILKQKAYSWEDELGINMIEFDIPADKVEEAQAAREQLVEYI
                     AGLDDDLTNRFLEGEELTNEMLIAGLRRAVIAGKCNPCYCGSSLKNKGVQLLLDAVVD
                     LLPSPLDIPPTKGTSLDGSKEIWLKPDDSAPVGSLVFKIVTDPYVGRLAYFRVYSGTV
                     KQGQMLLNPVKGKKERIGRLIRMYADRRDDVDEVYAGDIAAVLGLKDTFTGDTLCDPH
                     HPVVLESITFPEPVISVAIEPKTLADQEKIGLALQKLSEEDPTFRVRTDENTGQTIIS
                     GMGELHLAIIVDRLMREFRVRANVGKERVAYKETIRKAVEKHSYKYAKQTGGHGQFAH
                     VVLTVEPIDANLGVQFENKIVGGVIPKEYIPAVEKGVREAAESGVLGGYPVTGIRVSL
                     VDGSFHEVDSSEMAFKLAGSICLKECLELADPVLLEPIMKVEITIPEEYLGDVLGQIS
                     SRRGEIRGTDIHGGNSQIVHSMVPLGEMFGYATTLRSATQGRGVFAMEYDHYQAVSDA
                     TAQKILNI"
BASE COUNT         4081 a         3299 c         3289 g         4078 t
ORIGIN      
        1 tagtatcaat tctttgaaac gggcgggcgc aataccaggt ccgtttttct tttttcccga
       61 atccccgcag atccattgca ttgctgatta attcgattcg atccagactc gaaaaaatca
      121 acggtacggc aatgctcgaa attccggcta ttcgtttccg gagcgggaca tttttggaca
      181 agtcgacgcc gcgtgcctgt tgggattggg aaatgttatg ataatcactt tggatgtccg
      241 caatgtatcg caacgtgatg gaaaccgcat aggagatgcg gtaagaaata ccgattcgtg
      301 aaagcgatga ggcgaattcg ctcggatctg ttgtgacgat aaacaacagg gcaatcggga
      361 cgaccgtgaa atactttaac gttacattga acaggtaaaa aagctgttct ttggtaaccg
      421 tatagcggtt tgtaaattgg aaaagaacat gacgggtccc gtaaatcttg actccctctt
      481 ccggagagaa aaggtaaatc gtaatattgt tcagcaacag gaagaataaa ataaacaaca
      541 aaacaaaacg gacttcctcc aacttgattt ttgccaaata aaaaataatg atactcagag
      601 ccatgaccac aagcaacaca cgcgtatcat agctgagcat acaaacgatt gtccaaacta
      661 aaaatgtata taattttgtc acgccgcaca ggcgatgaat cggagaatcc cgtttaatat
      721 acgaaagcat ttttactgat gccatgtttt ccggaccctc ctatcataat cgatgaaaca
      781 ttggacaaag ttcagcgggt catctaagca ggctttttca gctaaggtaa ataaactggt
      841 ttccttcaga tccgccggtc ggataatatt gagatccgac aggacacgag cggggctgtc
      901 atcgcaaata agacgaccgt cggcaataac gatggcacgt cgggtatatt ccagcatcag
      961 atgcatatca tgagtaatca aaagaattgt caatccccgc tcttgaagag tttgcagaaa
     1021 ctccataatt tcggtgtaat ggcggaaatc ctgtccggca gtcggttcgt ccaataaaat
     1081 aatctgcggt tctaagacta agatggaagc gatcgttacc cgcttttttt gcccatatga
     1141 taatgccgag atcggccaat ttctgaaagg atacaggcca cagatttgta atacggcatt
     1201 aactctttcc tcgatttctt ggggatcgac atcccgaagt tttaatccca gtccgacctc
     1261 ttcccgaatc aatactttgg aaatcatctg attggggttt tgcatgacgt atccgacggt
     1321 atttgcacga tcgcgaatcg ataccccgtt caggttcttt ccgttcatga taatatcgcc
     1381 ggagcatggt ttttcgaaac cgcagatcag tttacaaagg gtactctttc ccgcaccgtt
     1441 tttaccgacg attgcggaca tttctccttt atgaatatcg aaacggatat gatgcagcac
     1501 ttcatgaagg ccgtcatagg aaaaactgac attttgcagc gaaagaatcg attcggcttg
     1561 tgattccgcc tccgaaggtg gaatctgttg ataccagcga aggagcggtt ggcgcacggc
     1621 atccattttg agcgtgttga tatgggatgg atgcatttcg ggtgtaattt gtattccggc
     1681 ataagaaagt gaggttatat aaagcggctc ccgaatacca tattgacgaa gtaatcctgt
     1741 tgaaagaatt tgatccggcg gttcatccgc cacgatacgt ccgtcattca tcacaatgac
     1801 acgatcaatc ggacaccaaa gaacatcctc cagacgatgc tcaacgatga taattgtttt
     1861 gcccgtctct tttgaaagac gatcgatcaa ttccattgta taacggccgg ttgccggatc
     1921 taaatttgcc aatggttcat cgaacagcaa gatgtcaacg tcatccacca gaactcccgc
     1981 caacgaaaca cgctgttttt gaccccccga aatttcctga ggggcgtttt ccaagtggtg
     2041 gttaatattt acgattttac tgacgcgatc tacaatatct ttcatttccg gttgcggggt
     2101 gcattcgttt tcaagtgcga aagcaatatc ttcccctacc gattggccga caaattggcc
     2161 gtccgaatct tgcagaaccg ttccgataat cttcgatcga tcaaaaattt ttaaattttc
     2221 ggctttttct ccgtgaatta acagttcgcc ggtactttct cctttgtaat aatgcggtat
     2281 taatccattg atacaatgga cgatcgtact tttaccgctt ccggatggtc cggcaattaa
     2341 gattttttca cctttagtga tccggaggtc gatattatat aaagttggtt tttcttgggc
     2401 tcggtaatga aaactgtaat ttttgaattc gataactggt tcagacatga aaataatttc
     2461 gttgaatgtc cttataaaaa ataaagggtg tccaaaagtt taatagagga caccctccga
     2521 atcgacagaa atgattttac tcctgtttta aacttcccgg ttttgttcgt gatcgagcat
     2581 aaattgccag aaatgcggtt ccgaggactg ctacggtgac gatatttgcg attcccgcaa
     2641 caattccctg agtaaatact ttatctgacg gctcggcata gatcaagata tccagaaccg
     2701 gagcgatcac tccccaaccg atcaggttcg ctactatttg gtaaatatta aaagtgagga
     2761 tttctttctt ttcgaatttg ccgtcttcga tattgatttt tttggcgcat aagccgattg
     2821 caaatccgac caaagccgag acaatcaccc agctccacca aaccgatccg tatgaaatgg
     2881 catccgttaa tgcgtggccg ataaagccga tcaaagcgcc ggcgatcgga ccgaaaatca
     2941 cagccatgaa agccaaaaat gcataagccg tgttaatggt tgtattgggg attccgcttg
     3001 gaatagccac gaaacgattc aatacgagga atacagcagc gccgattccg attgcaacaa
     3061 ttgtacgcgt tgtaagtttc atagatcact ccgatgatta tgtttttgat aacggacttg
     3121 ctcgataaaa aattatatca tagagggcgt ttgaaaactt aagaatcgaa tcattaaaat
     3181 atttccaatg gtgatgatgc gaaagcctat ttggtaaaac tggtcggtcc gtctcggcat
     3241 accaaccaag cttttttact gccgggttta tcggacggct cgtcccaggt atcgggcatt
     3301 tcaattttac aatttccgca gagtcccatc ccgcaacgca taatggcttc ccaggaaagt
     3361 tgtctggcta tattatattt ttcacagagt gcatcgatgc ccatcagcat tcccttcggc
     3421 ccgcaggcat acacacaggc atcctgcgga tccggcaaat tccgaatcga ttcttccagt
     3481 gggacagtga caaatccctg gataccgcag gaaccgtcat tcgttgttat atacattttc
     3541 agcgggagtt cttcaaattg atctttcaga attaacagat cttttgttcg tgcccctaaa
     3601 aacacctgaa cacgaatccc tttttcaatc agagcctgcg caaggaacaa caacggagag
     3661 gcaccatatc ctccgcctac caagagagca tctgttccgt aagcctgata tccctgtcca
     3721 aacgggccgc ggatccaaac tcgatctccc ggttttaacg catgaatttc gtgggtcagt
     3781 ttcccgacat ccgcaacggt gacgaccaaa ggatgggtgc cggcgatgct gaacggtttt
     3841 tcaccgactc ccggcagcca gagcaaaaga aattgtcccg gctcaccttc caaggattct
     3901 ttcatgatga agctgctcgt catctcgttt tcgtaacgaa ttcgatcgat tgtaaacaca
     3961 tgtgatcttt tttctttatt taagattgtt ttttccatcg gtttaccgcc atatttttcc
     4021 gactaactcc gaagcggatt gatatccttc ggatttcata aatgtttcga attcattcat
     4081 aatttgggag aatgcaccat atcccgaata atacagggcg gacccgatgc ccacaatcgt
     4141 tgctcccgcc attatcattt ccgccatatc caagccggtt aataccccgc cggttccgat
     4201 gacaggaata tttacagcct gataaatttc cgaaatacag tataaggcaa tcggtttaat
     4261 cgccggaccg ctgattccgc cgactccgga ggaaagaaaa ggctttgccg cgtaagcatc
     4321 cacgatcaat cctttcattg aattgattgc cgagatggca tcggctccgg catcctctat
     4381 gacttttgcc aattgtccta ttttcggata atcggcggat attttcaccg aaatcggaat
     4441 tccggaagcg ttttcgatga ctgcatgcgt aatttcctga gccagtgttt catccgggaa
     4501 cggtaaatca atttccagca aatcggggtg ggaaggggcg atcaaccgtg cgatttcagc
     4561 atattcttcc gcactttcac cgtggatatt tacaaatatc ggtatcgttt gatctcccat
     4621 ttcatttcgg aatgttttta tcatataggc catggattcg attcccgggt tgcttaagcc
     4681 taaattggtg atcagacccg ggccccaatc cgtggagaca ggattttccg cacctttctg
     4741 atatttcaat gtgacacctt ttgtggtaac ggcggccgca cctgatttgg cggctcgaac
     4801 caataaaccg tcactgttac cggaaatgcc ggatgccaga atcaacggcg attttaggat
     4861 cagattatca aacagcttac atgaaaaatt actcattcca tcggtccttt ataaccagaa
     4921 ttagagtcag tcaatgaacc aattctatca gaaggtgtga ttttccggat caaaatcaat
     4981 tcggatcttt gaatcgccgc tcttttcttt ccgatctcga ttaatgagcg atccgtttcg
     5041 aaaatctcat ataaaagaaa ttttattcaa tttacaaaaa gggagcggaa atttccgctc
     5101 cctttttgta ctgagaattc cgttaaaaat aatattcacc tttgttgcgt ttttcttctt
     5161 ctgactctcc gcccaaataa gccgcctgca ccttcggatc attcattaac tcttcacccg
     5221 taccgtttag cacaatctca ccggtttcaa gcacataacc gcgatcggca atcgccaagg
     5281 ccatttgtgc attttgctcg accagtaaga tactggtgcc tcgctgattg atatcaaaga
     5341 taatatcaaa aatctgttca accaaaatcg gcgaaagtcc catcgagggt tcatccagca
     5401 gcattaaatc gggatgtccc atcagacctc tggctatcgc aagcatttgt tgctcgcccc
     5461 cggaaagcgt accgccgggt tgatttcgcc gttctttgag acgcgggaaa agctgaaaaa
     5521 tattttccat atctcgatta accgaagctt tgttattatt taggaaagcg cccatctcca
     5581 gattttcctg aacggtcatt tttggaaaaa tatggcggcc ttccggaacc tgaacaatcc
     5641 ctttttggac gatgatatcc gtacccaaac cgattaattc ctcgtcttga taaatgatcg
     5701 aaccgccgct tgtctgaacc aaccccgata tcgaattgag agtggagctt tttccggctc
     5761 cgtttccgcc gataagcgcc acgatttcac ctttttggac agtcagagaa atgcctttta
     5821 aagcatgaat tgcgcgataa tgaacatgca gatctttgat ttcaaaatac atacatcttt
     5881 ccttattaac tctgatgaac tttttccgac ttgcgttttg aatatttttc aatcacattt
     5941 tttgttcccg aacccaagta agcttcgatt actctctgat ctttttggat ttcttcggga
     6001 ctgccctccg cgattcgttc tccgtggtcc agcacaagga tggaatctga aatattcatc
     6061 actactttca tatcatgttc aatcaggaca attgtgatct ttaattcaga ccgaagggta
     6121 ttgattaatt cgatcatcgc tttggtttca tgaggattca tgccggcaca aggctcatcg
     6181 agtaataaaa gatgcggatt gtttcccaaa gcccgggcaa tttccaaacg ccgttgggca
     6241 ccatagggta gattctttgc caaatcatcc gcataagcac tcaagccgac aaattcgagc
     6301 aaattgctgg ctttttgaat ggattgtttc tcttcgcggc gatttcgata agtctgtaaa
     6361 acggcatcca aacaattggc atgtaaacgg ggctgttgcc cgatcataac gttttccatg
     6421 acagtcatgt ttgaaaacaa acgaatattt tgatacgttc gtgatattcc catatcggcg
     6481 atttcatctg tcggaatacc gttgattggc ctttcctgaa acaaaacaac gccgctgttg
     6541 atcgaataaa aaccggaaat acaattgaaa aatgtcgttt ttcccgcacc gtttggtccg
     6601 ataatgctga tgatggattg atagggaacc tgtaaactga cattgtttac agctgtaagc
     6661 ccgccaaagg taatggttat attttgggct tccagaacat aaggtggttt ccgagtcatg
     6721 gtttaagatc ctcctttaat acttcaagag attggggttt caccgccaac tttctgcgtt
     6781 tggcaggtaa gagcccttcc ggccggatga ccatcatgat gattaagaga gcaccgaaaa
     6841 cgagcatacg ataattatcc aggtcacgta acaattccgg taacccttta ataatgaagg
     6901 cgcccagtaa cgcccccggg atggaaccca taccgccgat cacaacgaca gctaaggcgt
     6961 taaccgaaac catgaaagcg tattcattcg gacccgtata ggtatttctc gatacataaa
     7021 tcgctccggc tgcccccgcg ataatcgctc cgatcacaat cgcaagcact ttataaaagc
     7081 tggtggtgac cccgcatgcc tgagcgacgg tttcgtcttc ccgcatcgct tcccatgaac
     7141 gccctattct tgaatattgt aatctgtaaa caatataaat cacgacaagc aagctgataa
     7201 taatcagata aagaaaccag acattttcct ctaacggctt tcccaaaatt tttaccagaa
     7261 acgcaggctg atgaggttgc ccgatatctt ttacaccttg agggccgccg gtataatcgg
     7321 caagaatatt gctatttatc agaattcgaa taatctcggc aaacccgagg gttacgatcg
     7381 ctaaatagtc accccgcaga ttcataaccg gtaatccgac caataagcct ccgattccgg
     7441 aactgattaa agcgatgata aatgccggaa tgaagctcat ttcgatcccc aacggttttg
     7501 gcgcggtcaa caatgcaaaa gtataggcac cgatcgcata aaaggctgca taaccgaaaa
     7561 ccagttgtcc ggacaatccg acgatcaaat tcaagccgag gcctaaaatg gcataaataa
     7621 atacgattcc taatacggaa atagtataag cattgatgat catcggcaag gcgaaaataa
     7681 aaatgacgac gaatacgatg aaaagctttt tgataatcgg gtgtttttca cactttttct
     7741 gaatttgtag gttgttatat ccctttttca gggatcggaa aactccgttc aacttactcg
     7801 ctttttggat gacgatactg gcaaatgccc ctaaaacggc tccgccgacg gtggtagcca
     7861 tataaatgcg gatcgcgtta ttgatatcgg tttggaagag aaaatttcga atcacatccg
     7921 gagaaagcgc gtccagatat ttacgcagat caaccccatt attgagataa tttccgagga
     7981 ttgcgttcag tgtcgaaacc aagattcccg atacaagtcc cgataccaaa ccggaaacca
     8041 gcactttgat cacagcggat ttttcattcg caagatgatt tttacggaca aatgatgccg
     8101 tcgataagaa taaaattaat ccgataatga caatcatata agtcggattg acttctttga
     8161 cttttttatc acttacaaac attttttcga ggatgacagc aattgtggat gtgaaattga
     8221 tcatttcgaa aaatattaaa ataacgccgg caaccaatcc gttttttacg gctttcgata
     8281 ataatttatt catcatactt tctccacatt cgatgctgat ttaccaaata accccgacgg
     8341 tttcaaaatt agaaccagaa ttaatatcgt aaaggaaatc acatctttta actggaaatc
     8401 gattccgaga aaagcgggtc cgacggattc gacaattccc aaaatcattc cccccgccat
     8461 cgctcccggg atgtttccga ttccgccgag taccgcagca gtgaaggctt ttatgccggg
     8521 aaggaatccg acatagtggt tgaaaatacc gttatggagc ccccacataa caccggctgc
     8581 tcccgccaaa aaaccgctga tcaaaaaagt ttggctggta acgcgatcca aattgattcc
     8641 catcagttgc gcggtagctt tatcttgaga aacggcacga atggattttc ccaattttgt
     8701 tttttgaacg aaaaagtata acgcgatcat taataggatc gccaagacga ggctgaaaat
     8761 accagttttg gtaataatga tcatcccgtc ttccatatta atattcaatc cggaaccccg
     8821 tgaaagtatt tccgggtttt tataggtttt tttattcggc ccaaacatga tttgggcggc
     8881 cgtttcaaga aaaatagacg ctccgactgc actgatcagg ggggcgagtc gaacatgaga
     8941 gtttcgcagc ggacgataag ctattttttc caaaaaatat ccgctgacgg tggcgacccc
     9001 ggtccccatc aaaaatgcaa ggatgatcga taaaatggga taaacattta aaaagttact
     9061 gtcgccgatt gtcaggaagt cgagatattc aaaagtaaaa tatcccgcaa aacaacccaa
     9121 catcataata tctccgtgtg caaagttaat cagcatcatc acaccataaa ccaacgtgta
     9181 tccgatggcg atcaaagcat acatgccgcc gacaatcaac ccggtattga taaatcccaa
     9241 ccaataaaca ggcccgtatt ccgggcggat gatttggata atcaggcgat atacaaggaa
     9301 tacaccggca cagattccga caataagccg aagccagtta attctattga ctttcgtttt
     9361 ctgtatggtt gttggtgtgc ttttatctgc atccatataa gattatgact ccttatctga
     9421 tccgtattgt ttttataaag ttaataggaa agttttaatt tcggatcagt tatatttcct
     9481 tgtcattatt tcagatttcg attggggttt attctatcat ttgccttttc ttgttgtgcg
     9541 gaatttcgaa taactgatga ctgaaataat tactctacaa tgaaaaagtg cctttcctcg
     9601 aagtcgattc aggcaggaaa gacacttttt ctgtcccgct tctgatttta tcggagcggg
     9661 aatgactttg attaattatt ttgcgacgac ccacttgccg tctttgacaa catagaatgt
     9721 cgggccggag gcattgcatt caccgttttc actgcaggtg atttcgcccg ttattccctg
     9781 gaaaccggaa gttgatcgga cagctttgac taaatccgcc cgcggaacgt acagatttcc
     9841 gtcatcgccg acaaaagata cttcactgat ctgtttggca agaacctgaa taacatcata
     9901 agcgaaccac gggaaagtcg attttgatcc cggttcacgc ccgagagtct ctttatattt
     9961 cttgtcgaat gcttttttcg cttccgaatc agccggaaca gaggatgtac cgtagcaacc
    10021 ttcggcattc tttccggctt tctcgatgaa ttccgctccg aaagcgccgt catcactgaa
    10081 gacaggtgta cccgccagac cgacttgccc taattggttg gcaatgacgg ataattcagc
    10141 cgtataaccg ccgtagtaga taatttcggg tttcttggaa gcaatcgagg tcaaaactgc
    10201 agagtagtct gcttcgcccg gagtgatcgc ttcataagcg acgggttcaa cgcctaattt
    10261 aaccagcgta tcgcgaacgg tatcggcaat tccttttccg taatccatgc cgtcatgcaa
    10321 aaccgcaacc ttcttatagc ctaatgtatt gacgatgaat tctgcggcaa atttgccttg
    10381 ttcggcatct tggaaaacaa ttcggttgaa tactttattg ttttcggtca gattggcacc
    10441 ggtggcggaa ggagaaagca tcggaatgta gtacttttcc ataatcggga taatcgcggc
    10501 ggattcaccg gttaatacat taccggcaac tgcgacaaac gtatcatcgg tcacccattt
    10561 gttagcgatg gaaacagctg tttccggtga gccgacggta tcttccgcca aaagttccca
    10621 ctcgaatcct tccagaggct tcagatcgga aatcgatatt ttctcgctgt cggaaatatc
    10681 cacaccatac atggcgtatt caccggtaag aggacctccc ataccgattt taatggtatc
    10741 tcccggttta atcaccgcgc aaccaccttc cgcgtcacag cgggacttat cagccacgcc
    10801 tttggctttc atcacatcgg atgcgccagc ttcactgccg gatttttcga cactttcgac
    10861 ggctgttttt gcggtgtcaa cagcggtggc ggcagcttca cccacggtct tttccgcact
    10921 ctctacagct gtttcagcgg aattcgctgc ggtcgagacg atattctcca cagcttccac
    10981 ggcggcagcc gcctgggaac tcgtggttgc actgggttcg gttgtggcgc tgggtgcggt
    11041 tgtggcactg ggcgctgtgg tggcttcctc tacaacctca gttgccggtt tgggagtttc
    11101 tgtggcttta cttccgcatc cggtaagaat taaggtagag ataagcaata tactgactac
    11161 aaacaagaac gattttttca tatttctctc cttgaaaaaa tttagatttg ccctttaaaa
    11221 ttgttgaatt aatatatttc acccttgttt ttgcaaatta ggtttatttt tctcttattc
    11281 gattaacatt caattcaaaa ggacacttgt agtatctttt gcggattaat tatgcctccc
    11341 gcagtaattc agaatattac tcaagaaaaa aatgactgtc aagtcaatct gaatttctta
    11401 ttacaattta ttttatgatt ttctgagaat ggtttttatc aatctgtcgg gatattgatt
    11461 actccacttt ttctgtcatt attttctttc ggcggagcgg aatatttcct cacttgattt
    11521 gaatgtgata taatcttttg gctaatccgt gtcaaaccgg cttagtattt attgcgtgta
    11581 aataacctcg ggttgcactc ataaaaaaag aaacagccga gggaaagaaa aattaagtat
    11641 cttttagtta tggaggcttt ttgaagtgcc aacgattaat caattgatca gaaaaggtcg
    11701 gaagacccga tcagtgaaaa gtaaggcgcc ggcgttgcag tacacctata attccagcaa
    11761 ccagcgtcgt actaaagtag ctgagggcgc accgcaaaag cgtggtgtat gcaccaatgt
    11821 tcgtgcgact acgccgaaga agccgaattc ggctttgcgg aagattgccc gtgttcgttt
    11881 gtccaacggt ttcgaagtga ctgcctatat tcccggtgaa ggccattctc ttcaggaaca
    11941 cagtgtcgtt ctggttcggg gtggacgtgt cagagattta cccggtgttc gttatcatat
    12001 tgttcgcggt acattggaca cgaccggtgt agcgaaacgt ggacaaggcc gttcaaaata
    12061 cggcgtaaaa cgggctaaga aataaattag gagaagttga atgcgacgta ctaaaccaga
    12121 aaagcgggaa gttaccccgg atgttcgtta taacagtgct gttgtgacga atttaatcaa
    12181 tcgtattatg cataacggaa aacgaagtaa cgcagtcaga atggtataca gtgccatgga
    12241 tatcgtcagt caaaagacgg ggaaagacgg acttgaagtt ctggaaactg ctttacgaaa
    12301 tgtcggtcct gtgatggaag ttcgcccgcg gcgtgtcggc ggtgcaacct atcaagttcc
    12361 gatggaagta tcggcaaatc gaagacagac tctggccatg cgctggatta ttgatgcatc
    12421 acgttctcgc accggtaaag catttgatga atgccttgcg ggtgaattac tcgatgctta
    12481 taacaatacc ggcagcgcaa ttcggaaacg cgaagaaacc cataaaatgg cggaagctaa
    12541 ccgtgcgttt tctcattaca gagtttgaga aaaagaattg agtaatccta tatggctcgt
    12601 gaatatccgt tagagaagta tcgaaatatt ggcgttattg ctcatatcga tgcgggcaag
    12661 acgacaacaa ccgaacgaat tctgttttat accggaaaga cttatcgggt tggttcagtc
    12721 gatgagggta caactgtaac ggactggatg gatcaggaac gggagcgagg aattacgatt
    12781 gtttcggctg cggtcacagc ggaatggaaa ggatatcgaa ttaatttgat cgatactccc
    12841 ggacatatcg actttactgc ggaagtgcaa cgttctttac gtgtcctcga tggcggagtt
    12901 gttgtttttg atgcgactca aggggttgag ccgcaaagtg agacggtctg gcgacaggcc
    12961 gataaatata atgtgccgcg tatctgcttt atcaataaaa tggatcggac aggtgcttct
    13021 tatcatgatt cggtcgaatc cattcgaaat cgtttggggg cgaatccggt tgaaatgcaa
    13081 atcccgatcg ggtcggaagg aaatttttcc ggcgttattg acattttgaa gcaaaaggct
    13141 tattcatggg aagacgaatt aggcatcaat atgattgaat tcgatatccc tgcggataaa
    13201 gtcgaagagg cacaagccgc ccgtgaacaa ttggtggaat acattgccgg attggatgac
    13261 gatttaacaa accgtttcct tgaaggcgag gaattgacaa atgagatgct gattgccgga
    13321 ttgcgccggg cggtcattgc ggggaaatgc aacccctgtt attgcggatc ctccttaaaa
    13381 aacaaaggtg ttcagctttt gttggatgcc gttgtcgatc tccttccgtc acctttagat
    13441 atcccgccga caaaagggac cagcctggac ggttcaaagg aaatttggtt aaaacccgat
    13501 gattccgcac cggtgggttc tttggtattc aaaattgtca ccgatccata tgtcggacgg
    13561 ttggcatatt ttcgggttta ttccgggacc gtgaagcaag gtcaaatgct tttaaatccg
    13621 gtcaaaggga aaaaagaacg gatcggcagg cttatccgaa tgtacgccga tcgcagagat
    13681 gacgtagatg aagtgtatgc gggggatatc gccgccgtct tagggttgaa ggacacattt
    13741 actggcgata cgctttgtga tcctcatcat ccggttgtat tggaatccat cacttttccg
    13801 gaaccggtta tttcggtggc aatcgaaccg aaaacattgg ccgatcagga aaaaatcgga
    13861 ttggctctgc aaaaattgtc cgaagaagat ccgacgtttc gtgtccgaac ggatgaaaat
    13921 acgggacaga ccattatttc cggtatgggc gaactgcatt tggcgattat tgtcgatcgg
    13981 ttgatgcgtg aattcagagt tcgggcgaat gtcgggaaag aacgggttgc ctataaagag
    14041 acgattcgaa aagcggtgga aaagcacagc tacaaatatg cgaaacagac cggcggtcac
    14101 gggcaatttg ctcatgtcgt tctgacggtc gagccgattg atgcgaactt gggcgtccaa
    14161 ttcgagaata aaattgtcgg aggagtaatc ccgaaggaat atattccggc ggtggaaaaa
    14221 ggcgtccggg aagcggcgga atccggtgtg ttgggcggtt atccggttac cggaatacga
    14281 gtatcgttgg tggacggctc gtttcatgaa gtcgattcga gtgaaatggc atttaaactg
    14341 gccggcagta tttgtctgaa agaatgtttg gaattggcgg atcccgtatt gttggaaccg
    14401 atcatgaaag tcgaaattac gattccggaa gaatatttgg gagacgtatt gggacaaatc
    14461 agcagccggc gcggagaaat tcgcggaacg gatatccacg gcggaaattc gcaaattgtt
    14521 cattcaatgg ttccgttggg agaaatgttc ggatacgcga cgacactacg ctctgccaca
    14581 cagggccggg gtgtgtttgc gatggaatat gatcactatc aagcagtttc ggatgcaact
    14641 gcacaaaaaa tattaaatat ataatataag ttttctgagg agaatttgtt ttatggcaaa
    14701 acagcattat gagcgaaaga aaccacatgt gaatgtgggg accatgg
//
LOCUS       DAOSMM010000056        14685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4123_length_14685_cov_17.138483, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000056 DAOSMM010000000
VERSION     DAOSMM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4123_length_14685_cov_17.138483"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(967..2331)
                     /locus_tag="PK898_08035"
     CDS             complement(967..2331)
                     /locus_tag="PK898_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOU20019.1"
                     /translation="MEQRKYGLFTAIAMIIGIVIGSGIYFKSYNILYFTGGNILQGVI
                     ALCVGAISIIFGCLAVSELASRTDKAGGVITYSDKFWNKKMAIVFGWFHMWIYYPTLT
                     VIIAWISGVYLNILFGWENTLERQIINSVIVIALLFCINMFSAKLGGIFQRVTVVVKM
                     IPLILFGIVGLIFGNPGEAWTASQAAGTSGTWLQAIPAVLYAFDGWIIATSISHEIKD
                     SKRNLYLALIISPLIILALYLMYFIGMSSYMGPNQIIAMGNEHLYAAATKMMGTELGA
                     KLVLILVIISVLGVDNGMILGFIRLPYSLALRKMMPDSEKYAVIHPKHGVSLRSGFFA
                     FALSMIWVIAHYFTQKYIWLKGQHDISEIAMVVNYILFLTLYAAVIKLAIKGEIHSIW
                     RGWIIPILAMIGAGIIILSGFQNELFIWFLVFCAIVMGSAWLFAVKHPEIETVNLEEI
                     DLGE"
     gene            complement(2399..3358)
                     /locus_tag="PK898_08040"
     CDS             complement(2399..3358)
                     /locus_tag="PK898_08040"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOU20020.1"
                     /translation="MRGKEDPRLIARVARLYYEEGVKQNEIAEKLGLSQAAVSRLLAQ
                     GQKDGIIKVVVNIPRGVYTEIEEKLVEKYGLKDAVVADCSLADDGQGLAREIGYCTAS
                     YLENILRPNDIIGVSSWSTYLLAAVNAMTPINRKTGIQVVQILGGLGNAQSQSHSTHL
                     TSHLSEVVNGTPFFLPVPAVVKSAEIYKTIITDKSIQNIFAMFPFVNTALVGIGNMNP
                     SSLLAASGNVLSEEDSRILRKHGAIGDVLLHFYDKMGNEIDESSLTNHVISMSLSQLK
                     KVERAIGVAGGESKFDAILGALRGRLINILITDQFTAKRLLEA"
     gene            3535..4359
                     /locus_tag="PK898_08045"
     CDS             3535..4359
                     /locus_tag="PK898_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU20021.1"
                     /translation="MTIKELKEKSILYRETTLRIIRDSQSGHTGGDLSCIDILNVLYN
                     EILNISPDRVNDPDRDRYVQSKGHAAEALYAVLSENGFMPKEWTKNVGGYLSPATGHP
                     TKNIPGIEQNTGALGHGLPMAVGMAIAGKMDGRAYRVFTLMGDGELAEGTNWEASMAA
                     SHYQLDNLIVIVDRNGLQISGTTEEVMALEPLRAKFESFGFCVREVDGNDVAALCSVL
                     KEVPFEKGRPSLVIAHTIKGKGVSFMENQVQWHHAVPTDEQFRQAILELENERMAL"
     gene            4281..5312
                     /locus_tag="PK898_08050"
     CDS             4281..5312
                     /locus_tag="PK898_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU20022.1"
                     /translation="MASCGADGRTVPAGDFGTGERKDGALMLRPVEKGIANLECMASV
                     LTAHAEKDSSIIVVTSDSRSSGKVSGFARQFPRQLVEVGIAEQDSVGISAGLASCNKK
                     VFTVSPGSFLSARALEQVKNDVAYSQNPVALIGISSGVSYGGLGATHHSIHDLAVLQA
                     IPGLEVIVPADNNETEAALVSYLENPRPVYIRFGKKAMPRFYQAPDEVKIGKVSVLNE
                     GDDLLILAIGEPLWPAYDAAVALESSGVRCSLASVHTLCPIDRDTIIELASHSRGIIT
                     VEEHSVYGGLGSTVAALLMEAGIYRPMKIIGFPREEMVTGSQSDLFRHYGIDAENIRK
                     TAMDLLNRN"
     gene            5323..6804
                     /gene="glpK"
                     /locus_tag="PK898_08055"
     CDS             5323..6804
                     /gene="glpK"
                     /locus_tag="PK898_08055"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOU20023.1"
                     /translation="MTTILAIDQSTSATKAMLFNEQAELTEQVSIRHQQYFPKPGWVE
                     HDAEEIYQNLLRAVKELKEKAPQTFREISWLSITNQRETIVVFDQKSGKPLSHAIVWQ
                     DRRAETFCATLRNSGMGEVVHKKTGLIVDTYFPAGKLTWMKNNIPEVAEGLENGTALM
                     GTIDTYLIYRLTKGKVYATDHTNASRTLFYDIDALDWDRDLIGIFGLKIKELPEIQES
                     ASIFGETDFEGLSEKPLPIAGVMGDSQAALFAEHCYQPGMAKITFGSGSSILMNIGNV
                     RAQSDNGIVNAVAWVLNGQPTYAFEGITNFTGATISWLTDQLRLINNPAESEELAQSV
                     PDNSGVYFVPAFVGLGAPYWKADAKGAIIGLTPGVKKEHIVRAALEGIGFTVTDVLKV
                     MESEAGIPLTQLCADGGAVRNRFLMQFVADINHRDLRVSLLPELSALGAVFSGGLATG
                     VYRDTDAVQAINSGVIEYHPQLSIDSVETLYAGWNQAIKKVLL"
     gene            6819..7481
                     /locus_tag="PK898_08060"
     CDS             6819..7481
                     /locus_tag="PK898_08060"
                     /inference="COORDINATES: protein motif:HMM:NF021565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HOU20024.1"
                     /translation="MNKTWKRVIIAVLVIAFLVWGSTQFTVVNTEKQTAVLEAEKFDR
                     VAYVDAIWDSKLIPALQNDSKDLATVLNALSKGLEETKDYAMISVSGAYNFRVKGTGT
                     VESVDVTSQEGKAVIKVDGYDGNIKVILNIGPKINGESIRDGAGFINFGDFKDQTEYG
                     QVSKELNTRAGKIVSAALDWANMTGKRISFAGMFTIKTTNQTNLNIDTVTITPVIVEG
                     ID"
     gene            7484..9070
                     /locus_tag="PK898_08065"
     CDS             7484..9070
                     /locus_tag="PK898_08065"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU20025.1"
                     /translation="MASTQETEMIREPAEEIQPIEDEVILRAEQIVKVYPGTTALNGV
                     DFDVYRGKVNVLVGENGAGKSTLMKILAGVEQPTSGHIYLHGKEIFNRSIVEAGENGI
                     GIISQEMMIFPNLSVTENLFIGREKKNGIVVSAKEQREETVRILNRMEQKINPDQLVG
                     ELRVGQQQIIEIARAISEKKQILIMDEPTSALSNQEVRVLFKLIRDLLAGGVTIIYIS
                     HKLEELMQIGDYVTVLRDGNKVACAKIKDIDLPWIIRCMIGDSMKDQITFAPREIGEE
                     LMRVEDMTLPRVGGGFAVDHVSFTVHKGEILGLYGLVGAGRTELFECLCGYAEDAIGD
                     IYIRGKKIKDKRIDDRIRDGIILVPEDRQAEGLIPTMSVAGNIVLSSLKNYLKGFYLS
                     SALEKQAVEEKIKEINIKVADPDELVTSLSGGNQQKVVVSRALLTHPTILLLDEPTRG
                     IDVGAKAEIFQIVNDLAAQGYGIIFVSTELKEVITVSDRILVMSKGKITGDFMKENAT
                     EEALVTASAIGHRLEGGQYA"
     gene            9063..10127
                     /locus_tag="PK898_08070"
     CDS             9063..10127
                     /locus_tag="PK898_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20026.1"
                     /translation="MPEAQNSKISNRWLKIQSYLMKGRAFIALIIVIVVFVIIVPNFL
                     NSTNLVVMTKHVAIYGILAIGMTFVILTGGIDLSVGSVAGLCAMMAGGFINEGIRFGS
                     VTLYPSIPMVIILSALIGILCGLIDGFIIAKFNVAPFIATLGMWYIARGFAQIRSNGL
                     TFPNLVGRPELGNTGFPLLGAGSFLGISYSIWILILFILVSMFVTLKVPFGRHVYAIG
                     GNEKAARMSGVPIEKTKIAVYAISGFCSSIVGLIIASQLVAAHPMTGESYEMTAIAAV
                     VLGGASLSGGIGSIGGTIIGAFVIGILSDGLVMAGVSSFWQQVAKGAVIVVAVIIDQT
                     QRRMQERVALAQQLEEKESK"
     gene            10212..11192
                     /locus_tag="PK898_08075"
     CDS             10212..11192
                     /locus_tag="PK898_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU20027.1"
                     /translation="MKKKMLLFVTVLLVLSTVFSVTAAPAPAESKGLIYVITPSHANP
                     FFGAEAEIAVKRAEELGYTTKSVVHDDDANKQMELIETAIAEKAVAIILDNAGADATV
                     SAVKKAKDAGIPTFLIDREISEEGIAAAQIVSNNYQGASLVAEYFVKAMEEKGTYIEL
                     TGRDTDTNAHVRSQGFHDVIDEYPDMKMIAQETANWDQTEAFSKMETLLQANPDVKGV
                     VCGNDTMAMGAYAALVAAGKPDVIVVGFDGSDDVLQSIMDGGIKATGLQPVAAISIAA
                     VDQADYYLTHGEFQKEEKQSIDCVLITIENACMYKTFAPVEGVTSCEKKQ"
     gene            11342..12748
                     /locus_tag="PK898_08080"
     CDS             11342..12748
                     /locus_tag="PK898_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOU20028.1"
                     /translation="MKKITLAVLFGSREFFPGDLVIDARKQMFKALDLAGINYIALDE
                     KVGVHGAVTTYAHAKECAALFKQHMDEIDGILVSLPNFGDEKGIVDTIKLSGLRKPIL
                     VQAFPDDLDKFDVANRRDSFCGKISVCNNLRQYGYNFSLTTDHCVYPYEESFKEDLKK
                     FAGVCRVVNGLKHARLGAIGARPNAFNTVRYSEKLLENAGISVLTADLSEIIGPVYRL
                     SDSDPAVKKALEAIDAYIPHEGVPSEALVKMAKFKTVLDQWIRDNEITGTAIQCWNSV
                     QENLGVNVCTLMSMMSENLIPSACETDITGLLSMYTLQLAGDAPAILVDWNNNYAAKK
                     DECVLFHCGNFAKSYVPNGCMGNAKILATTMGTENTMGTIEGRVVGDKMTYARVSTDD
                     VNGMIRAYTGEGKFVDDELDTFGARAVVQIPDLQELMHFICLNGFEHHVAIAKENLAE
                     IVAEAFEGYMGWATYLHS"
     gene            12820..13218
                     /gene="rbsD"
                     /locus_tag="PK898_08085"
     CDS             12820..13218
                     /gene="rbsD"
                     /locus_tag="PK898_08085"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HOU20029.1"
                     /translation="MKKNGILHGEISKTIAELEHYDTILIGDAGMPCPDGVKWIDLAV
                     SEGVPSFFDVLTAVLSELEVQRAVISREMKDRSPEAYARLRDILENECPIEEIPHEEL
                     KQTSKKCKAHIRTGEFTPYCNVILEGGVTF"
     gene            13260..14174
                     /gene="rbsK"
                     /locus_tag="PK898_08090"
     CDS             13260..14174
                     /gene="rbsK"
                     /locus_tag="PK898_08090"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOU20030.1"
                     /translation="MNQPKICVAGSSNMDLVTSVKRMPLPGETIHGIDFHTFFGGKGA
                     NQAIMAAKLGGRVTMIAKVGNDGFGQQYLENYQSSGLVTDYVSVTDEAPTGIAAITVD
                     EQGQNSIIVVSGANFAMTPSDIENATPAVREADAALCQLEIPLDATIRFLEIAKDANV
                     LTIFNSAPAQELPEKIYALSDYFCPNETEAALMAGFPVKTIEDAEKAGRLFIGRGAKS
                     VIVTMGGLGSLFVSADKVFHVPAEKVKAVDTTGAGDAYIGSIAYFLASGASIETAMKN
                     ATHVAALSVQKSGAQTSFPSYADCGFDV"
     gene            14278..14365
                     /locus_tag="PK898_08095"
     tRNA            14278..14365
                     /locus_tag="PK898_08095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14312..14314,aa:Ser,seq:tga)
     gene            14385..14473
                     /locus_tag="PK898_08100"
     tRNA            14385..14473
                     /locus_tag="PK898_08100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14419..14421,aa:Ser,seq:gct)
BASE COUNT         3906 a         3123 c         3835 g         3821 t
ORIGIN      
        1 cccattcttt aattatccag ggggaaagct atcgccagat ttctcgcaga aaggaagcaa
       61 tcaaaaacgc taaattatag atttatgcct tctggtggta atcatccacc cgatcatgag
      121 tggtaaacat aatccgacta tgggtggtaa acttcgaccg gctattgaca catgctgagg
      181 tacgaggtac tgacgtcccg ttctgaataa aatgagacat ctatttttct gccctcaccc
      241 taaccctctc ccgtcgggcg agggaatttt tgaatgggat gacgtcctcg acgtcccgtt
      301 ctgaataaaa tgagacatct atttttctgc cctcaccctg accctctccc gtcgggcgag
      361 ggcattttgt aggggacggc gtcctcgacg tcccgcttag gtttttggct gggcgaggga
      421 attattggaa gtaagggcat ccgttacatc tcgcttagac atcaccaatt aatttttgaa
      481 tcgataccct caccctaacc ctctcccgtc gggcgaggga atttttgaat gagatgacgt
      541 cctcgacgtc ccgttctgaa caaaatgaga catctatttt ctgccctcac cctaaccctc
      601 tcccgtcggg cgagggaatt ttgtatggga cggcgtcctc gacgtcccac ttaggttttg
      661 gctgggcgag ggaatttttg gaggtaacgg cgtccgttac atctcgctta gacatcatta
      721 ataaattttt cggatcgatg ccctcaccct aaccctctcc cactgggcga gggaattttt
      781 gagtgggatg gcgttcgtta catctcgctt agacatcacc aattaatttt tcggatctgt
      841 gccctcaccc taaccctctc ccgctgggcg agggcatttt atagggggcc gccgtcccct
      901 atatctcata aatggttata tagaaatcga aatcgtattt tggtagttgc tcattcggcg
      961 gtgcgtttat tctcccaaat cgatttcttc gaggtttacg gtctcgattt cgggatgttt
     1021 gacagcaaac agccaagccg atcccatcac aatggcacaa aagaccaaaa accaaataaa
     1081 taattcgttt tggaacccag aaagaataat aatacccgcg ccgatcatcg ctaagatcgg
     1141 gataatccat ccgcgccaaa tactgtgaat ctcgcctttg attgccagtt tgatcaccgc
     1201 cgcatagagc gtcaggaaaa ggatgtagtt taccaccatg gcgatttcgg aaatgtcgtg
     1261 ttggcctttc agccagatat atttctgcgt aaagtaatga gcgattaccc aaatcatcga
     1321 taaggcaaac gcaaaaaatc cggaacgcag agaaacaccg tgtttgggat gaataacggc
     1381 atatttctcc gagtcgggca tcatttttct cagcgcgagt gagtaaggca gccggataaa
     1441 gccgagaatc atgccgttat cgactcccaa cactgagatg atcaccagaa tcaggaccag
     1501 tttcgctccc agttcggtcc ccatcatttt ggtagcggcc gcataaaggt gttcattccc
     1561 catggcaatt atttggttgg gacccatata gctgctcatt ccgatgaagt acatcagata
     1621 aagcgccaaa ataatcaacg gcgagatgat cagtgccaga tataaattcc gcttggaatc
     1681 cttaatttca tggctgatcg atgtggcgat gatccatccg tcaaaagcat acagcactgc
     1741 cgggatggct tgcagccaag tgccggaggt tccggcggct tgactggctg tccaggcttc
     1801 gccggggttc ccgaaaatca atccgacaat tccgaacaga atcagcggaa tcatcttgac
     1861 aacaaccgtt acccgttgaa aaataccgcc caattttgcc gaaaacatat tgatgcagaa
     1921 aagcagagca atgacaatga cgctgttgat aatctgccgt tcgagtgtat tttcccaacc
     1981 gaagagaata ttgagataaa ctccggaaat ccaggcgatg atgacggtta aggtcggata
     2041 gtagatccac atatgaaacc aaccgaacac aatcgccatc tttttattcc aaaatttatc
     2101 ggaataagta atcactccgc cggctttatc ggttcggctt gccaattccg aaacagccag
     2161 acagccgaag atgatgctga tcgctcccac gcacagagcg atcaccccct gcagaatatt
     2221 tccgccggta aaatataaaa tgttatagct tttaaaataa attccggagc ctatgacaat
     2281 cccgataatc atagcgatcg cggtaaacaa cccatacttt cgttgttcca tatgtttcct
     2341 ttcggatgga attaaaataa atgaaatata aaaacccgta caattataat ggtgattttt
     2401 aagcttccag caagcgtttt gcggtgaatt gatcggtaat taaaatatta atcaacctgc
     2461 cgcgtaacgc tcccaagatg gcatcgaatt tggattctcc gcccgctacc ccgatcgctc
     2521 gttcgacttt tttaagctgg gataaactca tggaaatcac atgattggtc aggcttgatt
     2581 catcaatttc attccccatt ttgtcataga aatgtaacag aacgtcaccg atcgccccat
     2641 gtttgcgcag gatgcgtgaa tcttcttccg ataagacatt tccgctggca gccaacaggc
     2701 tggaagggtt catgttgccg atcccgacca atgcggtatt tacaaaagga aacatggcga
     2761 agatattctg aatggacttg tctgtaatga tggttttata aatttcggcg gatttgacaa
     2821 ctgccggtac cggcagaaag aagggtgttc cgttgaccac ttccgataag tggctggtca
     2881 aatgagtgga atggctctgt gattgggcgt ttcccaagcc tcccaaaatc tggacgactt
     2941 gtatgccggt ttttcggtta atcggggtca tcgcattgac ggcggcaagc agataagtgc
     3001 tccaagaaga gacaccgata atgtcattgg gacgaaggat gttttccaga taacttgccg
     3061 tgcaatatcc gatttctctg gctaaacctt ggccgtcatc cgccaacgag cagtccgcca
     3121 cgacggcatc tttcaaaccg tacttttcaa ccaatttttc ttcgatttcc gtataaacgc
     3181 ctcgcgggat gttaacgacc actttaatga tgccgtcttt ctgtccctga gccaacagac
     3241 gggagactgc cgcctgcgaa agcccgagtt tttcggcgat ttcattctgt ttgacacctt
     3301 cttcgtaata gagacgcgcc acgcgggcaa ttaacctggg atcttctttt cctctcattg
     3361 agtgctcgtt ttcctgtcgg ttttcaatgt gacaaatgtt acaattattt aaaagattga
     3421 ataaatattc aaaaacgatt gattattcag ttttaaaaag gtatgatcaa ggtaaagaat
     3481 aatcaccgca cactgaataa atattcaaaa gcattttatc acgattgggg cattatgact
     3541 ataaaagaac tgaaagaaaa atcaattctt tacagagaaa caacactcag aatcatccgc
     3601 gattcgcaat cgggacatac cgggggagat ctttcctgta tcgatattct gaatgttctc
     3661 tataacgaga ttctcaatat atcacctgac agagtgaacg atccggaccg cgatcggtat
     3721 gtacaatcca aaggacatgc ggcggaggct ttatatgccg ttctttccga aaacggcttt
     3781 atgccgaagg agtggacgaa aaatgtcggc ggatatcttt caccggcaac cggacacccg
     3841 acgaaaaata ttccgggaat tgaacagaat acgggtgctt tagggcacgg tctcccgatg
     3901 gcggtcggta tggcgattgc cggaaagatg gacggaaggg cgtatcgcgt ttttaccctg
     3961 atgggagacg gtgaattggc ggagggtacc aattgggaag cctccatggc ggcgtctcac
     4021 tatcaattgg ataatttaat cgtcattgtc gatcgaaacg ggcttcaaat ttccgggacg
     4081 acggaagaag ttatggcgct ggaaccattg agagccaagt ttgaatcatt tggtttctgt
     4141 gtcagagaag ttgacggaaa tgatgtggca gccctttgtt ccgtattaaa agaggttccg
     4201 tttgaaaaag gcagaccgag tttggttatc gctcacacga taaaaggaaa aggggtcagt
     4261 tttatggaaa atcaggtcca atggcatcat gcggtgccga cggacgaaca gttccggcag
     4321 gcgattttgg aactggagaa cgaaaggatg gcgctctgat gttaagaccg gttgaaaaag
     4381 ggattgccaa cctcgaatgc atggcatccg ttctgacggc gcatgccgaa aaagactcaa
     4441 gtatcatcgt agttaccagt gactcccgca gttccggaaa ggtaagcggt ttcgcccggc
     4501 aatttccgcg acaattggtt gaagtcggca tcgcagaaca ggattcggtc ggtatatcgg
     4561 ccggactcgc ctcttgcaat aaaaaagtat ttaccgtttc cccgggcagt tttctctctg
     4621 cgcgagcttt ggaacaggtt aaaaatgatg ttgcatattc tcaaaatccg gttgctttga
     4681 tcggaatcag ttccggtgtg agttacggcg gtttgggcgc gactcatcac tcgattcatg
     4741 atctggctgt cttgcaggca atccccggtt tggaggtgat tgttcccgcg gataataacg
     4801 aaacggaagc ggctttggtc tcttacttgg aaaatccccg accggtttat attcggttcg
     4861 gtaaaaaagc gatgccgcgc ttctatcagg cgccggacga ggtgaaaatc ggaaaggtct
     4921 ccgttctgaa tgaaggggac gacttgttaa tccttgcaat cggtgaaccg ttatggccgg
     4981 catacgatgc ggcggttgcg ctggaaagca gcggcgttcg ctgttcctta gccagcgtcc
     5041 acacgctttg cccgatcgat cgggatacga ttattgaact ggcttcacat tcccgtggaa
     5101 tcataaccgt tgaagaacat tcggtttacg gcggtttggg aagtacggtt gccgcgttgc
     5161 tgatggaagc cggaatttat cgtccgatga aaattatcgg attcccgcgg gaagaaatgg
     5221 tcaccggcag ccagtccgac ttgttccgcc attatggaat cgatgcggag aatatccgca
     5281 aaaccgcgat ggatttatta aacagaaact gagtcatcgg ctatgacaac catattggca
     5341 atcgatcaaa gtacttccgc caccaaagcg atgctgttta atgagcaggc ggaattaacg
     5401 gaacaggtca gtatccggca tcaacaatat ttcccgaaac ccggatgggt tgaacatgat
     5461 gccgaagaaa tctatcaaaa tctgctccga gccgtcaaag aattaaagga aaaagcaccg
     5521 cagacttttc gagagatcag ttggttgagc attaccaacc aacgggaaac gattgtggtt
     5581 tttgatcaga agagcggaaa gccgttgagc catgcgattg tttggcagga tcgccgcgct
     5641 gaaacattct gtgcgacttt gcgaaattcc ggaatggggg aagtggtcca taaaaaaacc
     5701 ggtttaattg tggatactta ttttcccgcc ggtaaattaa cctggatgaa gaacaatatc
     5761 cccgaagtgg cggaaggatt ggaaaatggg acggctctga tgggcacgat cgatacctat
     5821 ctgatctatc gcttgacaaa agggaaagtc tatgcgaccg atcataccaa tgccagtcgc
     5881 acccttttct atgatatcga tgctttggat tgggatcggg atttgatcgg gatttttggt
     5941 cttaaaataa aagaattacc cgaaattcag gaaagcgctt ccatattcgg cgagacggat
     6001 ttcgaaggat tgtcggaaaa accgcttcct atcgccggcg taatggggga ttctcaggcg
     6061 gctttatttg ccgagcattg ttatcaaccg ggtatggcaa aaattacttt cggcagcgga
     6121 tcatccattc tgatgaatat cgggaatgtg cgggcgcaat cggataacgg gatcgtcaat
     6181 gcggtggcat gggttctgaa cggacagccc acttatgcat ttgaagggat tacgaatttt
     6241 accggtgcga caatttcctg gctgacggat cagcttcgat taatcaataa tcccgcggaa
     6301 tccgaggaat tggcgcaatc ggttcccgat aattccggag tatattttgt ccccgcattt
     6361 gtcggcttag gtgcgccgta ttggaaagca gacgcaaaag gggcgatcat cggtttaact
     6421 cccggcgtta aaaaagaaca tatcgttcgc gcggctttgg aagggatcgg gtttacggtg
     6481 acggatgtat tgaaggtgat ggaaagcgaa gccggaattc cattgacgca actgtgtgcg
     6541 gacggaggtg cggttcggaa tcgctttttg atgcaattcg tagcggatat taatcatcgg
     6601 gatctccggg tatctctcct gccggaatta tcggctttgg gggcggtatt ttcgggaggg
     6661 ttggctaccg gtgtctatcg ggacacggat gccgtgcaag cgatcaacag cggtgtgatc
     6721 gaatatcatc ctcagttgtc gattgattct gtggaaacgc tatatgccgg atggaatcag
     6781 gcaatcaaga aagttctgtt atagggaagg aaacggctat gaataaaacc tggaagagag
     6841 tgattattgc cgtgttggtt attgcttttt tggtttgggg gtcgacgcaa tttacggtag
     6901 ttaatacgga aaaacagaca gcggtcctcg aagccgagaa atttgatcgg gttgcctatg
     6961 ttgatgccat ttgggattcg aagctgattc ccgcactgca gaatgactcc aaagatttgg
     7021 cgacggttct caatgccctc tcaaaagggc tggaagaaac gaaggattat gccatgatct
     7081 ccgtcagcgg cgcgtacaac ttccgagtaa aaggaaccgg aacggtcgaa tcggtggatg
     7141 tcacttcaca agaagggaag gcggtcatca aggtggacgg gtatgatggc aatatcaaag
     7201 tgattttgaa tatcggcccg aagattaatg gggaatccat ccgggacggt gcggggttta
     7261 ttaatttcgg cgattttaaa gatcagaccg aatacggaca agtgtccaaa gaattaaata
     7321 cccgtgccgg aaaaatcgtc tccgccgctc tcgattgggc gaatatgacc ggtaaaagga
     7381 tttctttcgc cgggatgttt acgatcaaaa ccacgaatca gacgaatttg aacattgata
     7441 ccgtaacgat aacccctgtt attgtggaag gaatagattg aaaatggcat cgactcaaga
     7501 aactgaaatg attcgggaac cggctgaaga gattcaaccg atcgaagacg aagtcatttt
     7561 acgggctgaa cagatcgtta aagtgtatcc gggcacgacg gctttaaacg gagtggattt
     7621 tgacgtctat cgcgggaaag tgaatgtctt ggtcggggag aatggtgccg gtaaatcaac
     7681 attaatgaag atccttgccg gcgtcgagca gccgacttcg gggcatatct atttgcatgg
     7741 gaaggaaata tttaaccgtt cgattgtcga agcgggagaa aacggtattg gcattatttc
     7801 tcaggaaatg atgattttcc cgaacctttc cgttacggag aatctgttca tcgggcggga
     7861 aaagaaaaac ggtattgttg tctccgcgaa agaacagcga gaagagaccg tcagaatcct
     7921 caatcgcatg gaacagaaaa tcaaccccga tcagttggtg ggggaacttc gggtgggcca
     7981 acagcaaatt atcgaaatcg cccgagcgat ttccgaaaag aagcaaattc tgattatgga
     8041 cgaaccgact tctgctttgt ccaatcagga agtgagagtt ctgtttaagc tgattcgtga
     8101 tttattagcc ggcggtgtaa cgattattta tatctcgcac aagctggaag aattgatgca
     8161 aatcggcgat tatgtcacgg tcttgcgcga cgggaataaa gtggcttgcg cgaaaataaa
     8221 agatattgat ttgccttgga taatccgttg tatgatcggg gactcgatga aagatcagat
     8281 cacttttgcc ccccgcgaga tcggggaaga gttgatgcga gtcgaagata tgacgctccc
     8341 tcgagtcggt ggcggatttg ccgtggatca tgtcagtttc accgttcata aaggcgaaat
     8401 attgggcctc tatggattgg taggcgcggg gcgaaccgaa ttgtttgaat gtctttgcgg
     8461 gtatgccgaa gatgcgatcg gggatatcta tattcgcgga aaaaaaataa aggataaaag
     8521 gattgatgat cggattcggg acggcattat tttggttccg gaagatcggc aagccgaggg
     8581 actgatcccg acgatgtcgg ttgccggaaa tatcgttctt tccagtctga aaaattatct
     8641 gaaaggcttt tatctctcaa gcgctttgga aaaacaggct gtcgaggaaa aaattaaaga
     8701 aatcaacatc aaagttgccg atcccgacga gcttgtgact tctttgagcg gcgggaacca
     8761 gcagaaggtg gtggtttcca gggcgctgct gactcatccc acgattttgt tgttggatga
     8821 accgacccgc ggtatcgatg tgggcgcaaa agctgagata ttccagattg tgaatgattt
     8881 ggcggctcaa gggtacggca tcatttttgt ttcaacggaa ctgaaagaag ttattacggt
     8941 atcggatcgg attctggtga tgtccaaagg aaaaattacc ggtgatttta tgaaggagaa
     9001 tgcgacggaa gaagcgttgg ttaccgcatc cgcgatcggt cacagacttg aaggagggca
     9061 atatgcctga agcacagaac tcaaaaataa gcaacagatg gttgaagatt caatcctatt
     9121 tgatgaaagg tcgggcgttt atcgcattga tcattgtcat cgtcgttttt gtcattattg
     9181 ttccgaattt tttgaacagc accaatttgg tggtgatgac aaaacacgtc gcaatttacg
     9241 gaattttagc aatcggaatg acatttgtca ttttgaccgg ggggattgat ctttcggtgg
     9301 gttctgtggc agggctgtgt gccatgatgg ccggcggatt tattaatgaa gggatcagat
     9361 tcgggtctgt tacgctttat ccttctatcc cgatggtaat cattctttcc gcattgatcg
     9421 ggatcctgtg cgggctgatt gacgggttca ttattgccaa gtttaatgtg gctcccttta
     9481 tcgcgacgct gggaatgtgg tatatagcgc gtggttttgc acagattcgc tccaacggat
     9541 tgactttccc gaacttggtc gggcgcccgg aattgggaaa taccggtttt cctttgctgg
     9601 gagcgggttc cttcctcgga atcagttaca gtatttggat actgattctg ttcatacttg
     9661 tttcgatgtt tgtcacgctg aaagtacctt tcggtcggca tgtgtatgcg atcggcggaa
     9721 atgagaaggc ggcacgaatg tcaggcgttc cgattgaaaa aaccaagatt gccgtttatg
     9781 cgatttcggg tttttgttct tcgatcgtgg gattaatcat cgcttcgcaa ctggttgcgg
     9841 ctcatccgat gaccggtgaa tcctatgaaa tgaccgccat cgccgcggtt gttctcggcg
     9901 gagcttcgct ttcgggcgga atcggcagta tcggcggaac gatcatcggc gctttcgtaa
     9961 tcggcattct ttcggatggg ctggttatgg ccggagtttc ttctttctgg caacaggtgg
    10021 caaaaggcgc ggtgattgtc gtggcagtaa ttattgacca gacacaaagg aggatgcagg
    10081 aaagagttgc gttggcacaa caactggaag aaaaagagtc aaaataaaag gtttattgat
    10141 ctaaatcggg tgggatacga aagctttttc gtataaaaaa atttgaaatt tttagtgaaa
    10201 ggaactaaaa aatgaagaaa aaaatgttgt tgttcgttac tgttttattg gtgctcagca
    10261 cagtgttttc cgttacagcc gctccggctc ccgctgaaag caagggtttg atttatgtga
    10321 tcaccccgtc tcatgcgaat ccgtttttcg gcgccgaagc agaaatcgcc gttaaaaggg
    10381 ctgaagaatt aggttatact accaaatcgg ttgtccatga cgacgatgcg aataagcaaa
    10441 tggaattaat tgaaaccgcc atcgccgaaa aagcggttgc catcattttg gataatgccg
    10501 gtgcggacgc aactgtcagc gcggtcaaga aagccaaaga tgccggaatt ccgaccttcc
    10561 taatcgatcg cgaaatcagt gaagaaggta ttgcggccgc ccagattgtc tccaataact
    10621 atcagggagc ttctttggtc gctgaatact ttgtcaaagc aatggaagaa aagggaacct
    10681 atatcgaatt aaccggacgt gatacagaca ccaacgctca tgttcgttca cagggattcc
    10741 atgatgtgat cgatgaatat ccggatatga agatgattgc gcaagagacg gcaaactggg
    10801 atcaaaccga agccttctcc aagatggaaa cgctgcttca ggcgaatccg gatgtcaaag
    10861 gcgtagtttg cggaaacgat actatggcaa tgggcgcata tgccgcattg gttgccgccg
    10921 gaaaaccgga tgtgatcgtt gtcggttttg acggttcgga tgatgttctg cagtccatta
    10981 tggacggagg aatcaaagcg acgggtcttc aaccggttgc cgctatttcc attgccgccg
    11041 tcgaccaggc tgattattat ctgacccacg gcgaattcca gaaggaagaa aaacagtcta
    11101 tcgactgtgt gttgatcacc atcgagaatg cctgcatgta caagacattt gctccggtgg
    11161 aaggtgtcac ttcctgcgag aagaagcagt agttgattgg caattcaggg acggcttgag
    11221 aaaagccgtc cctgatattt ttcaccagct ttcattttga aaataaggct ggtgaaaaat
    11281 atttggttaa aattagaata tattcaatag aataattcgt atgtccttgt gaggagaaca
    11341 aatgaaaaaa attaccttgg ctgtcttatt tggatctcga gagtttttcc ccggagatct
    11401 tgtaatcgat gcacgcaaac aaatgtttaa agcgttggat ttggcgggaa tcaactatat
    11461 tgcgctggat gaaaaagtgg gtgttcacgg cgctgtgaca acttatgccc atgccaaaga
    11521 atgcgccgct ttatttaaac aacatatgga tgaaatcgac ggaattttgg tcagtctgcc
    11581 caatttcggc gatgaaaagg gaattgttga caccatcaaa ttgtccggcc tgcggaagcc
    11641 gattttggta caggctttcc cggacgattt ggataaattc gatgtggcaa atcgccggga
    11701 ttctttctgc ggaaagattt cggtttgcaa taatttgcgc cagtatggct ataatttttc
    11761 tctgactacc gatcattgcg tttatccgta cgaggaaagt ttcaaagagg atctgaaaaa
    11821 atttgccgga gtttgccgtg tggtgaacgg tttaaaacat gcccgactgg gggcgatcgg
    11881 cgctcgcccg aatgcattta ataccgttcg atacagcgaa aaattgttgg agaatgccgg
    11941 tatttctgtt ttgacggctg atttgtcgga aattatcgga cccgtctatc ggctttccga
    12001 tagtgatccg gcggtcaaaa aagcgcttga ggcgattgat gcttatatcc cgcatgaggg
    12061 agtcccgagt gaagcgttgg tcaaaatggc aaaatttaag actgttttgg atcaatggat
    12121 tcgggataat gagataacgg ggacagccat tcaatgctgg aattccgttc aagagaacct
    12181 gggtgtcaat gtctgcacgc tgatgagcat gatgagcgaa aacttgattc cttccgcttg
    12241 cgaaacggat atcaccggtc tgctttcgat gtacactctg caattagccg gagatgcacc
    12301 ggcaatcttg gtggattgga ataataatta tgccgctaag aaagatgaat gcgtcttgtt
    12361 ccattgcggg aactttgcga agagttatgt gccgaatggc tgcatgggca atgctaaaat
    12421 tctggcgaca acaatgggaa ccgaaaatac gatggggacg atcgaaggac gggttgtcgg
    12481 agataaaatg acctatgccc gtgtttcaac ggacgatgtc aacggaatga ttcgtgccta
    12541 taccggcgaa gggaaatttg tggacgatga actggacact ttcggtgcca gagcagtggt
    12601 gcagatcccg gatttacagg aactgatgca ttttatctgc cttaatggct tcgagcatca
    12661 tgtcgcgatc gcaaaggaaa atctggctga gatcgtggct gaagctttcg aaggatacat
    12721 gggctgggcg acttaccttc acagttgaat ttcgatcgat gatcgatagt gattttcgtt
    12781 ccgttctgtt ggtgaacgga acggggggag gttaaataaa tgaagaaaaa cggtattctc
    12841 cacggagaaa tttcgaaaac catcgctgaa ttggagcact atgataccat tctcatcggg
    12901 gatgcgggaa tgccttgccc ggatggcgtc aagtggatcg acctggcggt tagcgaaggg
    12961 gtcccgtcgt tttttgacgt actgacggct gttttaagcg aactcgaagt tcaaagggct
    13021 gttatcagcc gtgagatgaa agatcgcagt ccggaggcgt atgctcgcct gcgggatatt
    13081 ttggaaaacg aatgtccgat cgaagagatt ccgcatgagg aattgaaaca gacatccaaa
    13141 aagtgtaaag cccatattcg taccggcgaa tttacaccgt attgcaatgt aatccttgag
    13201 gggggcgtca ctttctgaac cgcccctcca aattattgtt cgcaagagaa ggaacctcta
    13261 tgaatcagcc caaaatctgt gttgccggat cttccaatat ggatctggtc acgtcggtaa
    13321 aaagaatgcc cttaccgggg gagaccatcc atggaattga ttttcatact ttcttcggag
    13381 gaaaaggagc gaatcaggct atcatggcag ccaaattggg cggtcgggtg acgatgatcg
    13441 caaaggttgg caacgacgga ttcgggcagc aatatcttga aaattatcaa agttcgggct
    13501 tggtgacgga ttatgtttcc gttacggatg aagcccccac cggaatcgcg gcgatcaccg
    13561 ttgacgagca gggacaaaat tcaatcatcg ttgtcagcgg ggcaaatttc gcgatgactc
    13621 cgagcgatat tgaaaacgcc actccggctg ttcgggaggc agacgcagcc ctttgccagt
    13681 tggagattcc gctggatgcg acgattcgat ttttagaaat tgcgaaggat gcgaatgtct
    13741 tgacgatttt caattcggct ccggcacaag agctgccgga aaagatttat gctttaagtg
    13801 attatttttg tcccaatgaa accgaagcgg cactgatggc aggatttccg gtaaaaacga
    13861 tcgaagatgc cgaaaaggcg ggaagattat tcatcgggag aggtgcaaaa tccgtaattg
    13921 ttactatggg cgggctggga tctttattcg tttccgcgga caaagttttc catgttccgg
    13981 ctgaaaaagt gaaagcggtg gatacaacag gtgccgggga tgcgtatatc ggcagcattg
    14041 cctacttttt ggcatccgga gcatcgattg aaaccgcaat gaaaaatgcg acacatgtgg
    14101 cggcgttaag cgttcagaaa agcggtgccc agaccagttt cccgagttat gccgattgtg
    14161 gttttgatgt ttgaagattt gcttgacagc ggagtgacgg gataaatcaa ccgccggcat
    14221 cgaacggaga ccttccttgc cgtggttatg gaaacatgat aaaatcggtt cgttatcgga
    14281 gagatgaccg agtggttgat ggtgcctgtc ttgaaaacag gtgtggggaa actcaccgtg
    14341 ggttcgaatc cctctctctc cgccataatg ctccgttgaa acagggggag gtgccagagt
    14401 ggttgattgg ggccgcctgc taagtggttg ttggggtaaa actcaaccga gggttcgaat
    14461 ccctccttcc ccgctttttt atcctattat ttggaaatgc ttttctatat ggtttaaagt
    14521 cgagatatga atacctcttg taatgtagta ttcgttcgga gtgcttcgca aggacacaaa
    14581 aattccctct cccgccggga gagggtcagg gtgagggcac agatctcaac atttgattgg
    14641 tgatattcaa gcgggacgtc tgcgcctcgt aaactcggcg tgctt
//
LOCUS       DAOSMM010000057        14372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4240_length_14372_cov_15.156108, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000057 DAOSMM010000000
VERSION     DAOSMM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 14372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14372
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4240_length_14372_cov_15.156108"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="dinB"
                     /locus_tag="PK898_08105"
     CDS             complement(<1..795)
                     /gene="dinB"
                     /locus_tag="PK898_08105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOU20031.1"
                     /translation="MAKYRKILHLDLDAFFCAVEELLNPSLKGKKFAVGGSPDRRGVV
                     STCSYAARKCGIHSAMPMSQAIRRCPDLIIVHSGFEYYREKSREVMAILQHFTPLVEQ
                     ISIDEAFLDVTDLPQPIEEIARSVQKEINQKPHLPCSLGAASNKLVAKIADNIGKKRV
                     ITDTYPNAILWIPNGKEANFLSPLKVDELWGVGKKTAQIFENLGISTIGQLAAYPVSL
                     LVPYLGKSAAEYHNRALGIDDSPVEPEPAAAKSISQETTFERDVSDE"
     gene            complement(839..2179)
                     /locus_tag="PK898_08110"
     CDS             complement(839..2179)
                     /locus_tag="PK898_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOU20032.1"
                     /translation="MKISQPIARINFFTFITSHYNLGKNEKNGEKLSPAYNKEEITIT
                     NFSSDGKAVGKLSDGRVVFVPGTIPGERVLIRYSKSDKRYVQAELIEILSYSPKRTSS
                     RCRYFGTCAHCRLQHLNYSDQTEVKRNILIDQLKRIGKMGHPESLIQPTIPSDPYYGF
                     RKNMIFSVLPDGKLGLQDVSGGMFVADRCPICSEGINELIRQISFEAESGIHAAEFRE
                     GVEGELQIVLRGDADRPPMEIETDLSVSLVYSGPVGSFVLSGNSTLVQEIAGQEICAS
                     EDSPFFPNPEIYDALCQKLIEILPEDRDKPVLNLNCGTGFWAKWFASQGRKVIAQDVG
                     ERSADDFVVNLDAFDSVDLYLGKPESIIPAFRENKPLTVFEAASEGISVATAQALSKA
                     EVSRILYISHDSAILARDTIRLSDSGYKLNAIIPFDDEPQTAIISAAAIFRKNR"
     gene            2133..2975
                     /locus_tag="PK898_08115"
     CDS             2133..2975
                     /locus_tag="PK898_08115"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HOU20033.1"
                     /translation="MNVKKLMRAIGWEIFILLCGSLAGCHPVSNQAVGSESVHVTLPP
                     PQQIQLPSLTPFSAVEISTVTFLPENRSKSATLPAFFQTPTLKVVSGIEPDSKEYDVP
                     LVTQDGLKYKGVKTTLGCTAASVQMVLDYWKKVNPDFKTISAQKIIDINTSQGTFRTG
                     EGLSIENVKDELQNIGYILEIYQNSDKEQLLSVFDQFGPQILLVKTGWVPTGANHLVV
                     LVAYDKETDTVTVNDPWFDHPFTWDWEAFDGIWSLNYSQKENGYLTRSFFNIRPASLS
                     DNSD"
     gene            complement(2972..4522)
                     /locus_tag="PK898_08120"
     CDS             complement(2972..4522)
                     /locus_tag="PK898_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOU20034.1"
                     /translation="MKKNRFILLFLCLFLSIPLKVLAQSDSASITNYVSNRMQYKLIS
                     EAQCASDLASFYGYSIEPLEIMDKIPLTDDPNTGYVGYYDDEGGLPPNSYGVYQQPLS
                     KALQTLGVPAYGYQNYSLDQLKTSVRQGNPVICWVVGNTAAGTPVEYTPSSGQSTTVA
                     LHMNSVTVTGFDQSGLLIWDNGESYTRSYEAFEASWGVLGNRALIIMPDKTASDKTDW
                     WGQPGDEFWDSEQTADEPTPLPEITIQKYPVTETEDYFSFDTEQDQNDQFGVIPEVTP
                     DFDWNSSPNSEDYVGWFWFPEDESADNYNYDNNTFMPFSGSFGNLNDIWDYSQPLPTQ
                     AYVDGFVGHAQSYPLDCETRSAVDFAAYFGFSIDAYQFLTSLPKSDDPNEGFVGNYTD
                     PRGQIPPASYGVYQEPVAALLRSYGVPAVGYKNMSWERIKYEISNGRPVMVWVAGNTE
                     VGTPQYYTPSNGKSTKVVPYQHTVVVIGYDSQNVTIQDGAMRYTRSINTFLTSWATLD
                     NRGIIAVY"
     gene            complement(4554..5021)
                     /locus_tag="PK898_08125"
     CDS             complement(4554..5021)
                     /locus_tag="PK898_08125"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU20035.1"
                     /translation="MRSQSEGVLSSQFIPPTLVATSFKTPTTDPFQPTIDPRGPCTND
                     LTFIDDLTVPDGTVFEPGAEIVKRWQVENSGTCEWTAKYSVRILSGASMGAEPKQKLE
                     PIAPGETGSVEIVFHAPEETGEYYSQWQAYDDRGKPFGQDFFIDIIVSSQTGY"
     gene            complement(5186..6280)
                     /gene="recA"
                     /locus_tag="PK898_08130"
     CDS             complement(5186..6280)
                     /gene="recA"
                     /locus_tag="PK898_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOU20036.1"
                     /translation="MPKRSADQFSQPYGEIDPDAKRMMLDKALTDISKRFGEGSIMRL
                     GDAKHMEVEAISTGSISLDLALGVGGIPRGRITEIYGPESSGKTTLCLHIIAEAQKMG
                     GTAAFIDMEHALDPKYAMRLGVNINELYVSQPDTGEQALEICETLVRSGAVDVVVVDS
                     VAALVPRSEIEGDMGDSSMGVQARLMSQALRKLSGAINQTKTAVIFTNQLRLKIGVMF
                     GNPETTTGGQALKYYSSVRLDIRRINSIKSGAEVVGSRVRVRVTKNKVAPPFLTAEFD
                     IMYNEGISRTGDIVDLGTQLEIITKRGSFYSYNDIRLGQGRENAKDFLKANPELANEI
                     EEKIRNAALGSDIPILSFSSDSEEDSSEEE"
     gene            complement(6372..8033)
                     /locus_tag="PK898_08135"
     CDS             complement(6372..8033)
                     /locus_tag="PK898_08135"
                     /inference="COORDINATES: protein motif:HMM:NF008183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOU20037.1"
                     /translation="MRKSWWKECVFYQIYPRSFKDSNGDGIGDLNGITEKLDYLKWLG
                     VGAVWLNPVYDSPNDDMGYDIRNYEKIMDEFGTMADFDRLLSEMHRRGIRLIMDLVVN
                     HTSDEHAWFVESGKSRNNPYRDYYIWRPGRNGKEPNNWASFFTPSAWKYDAATDEWYL
                     HLFSEKQPDLNWENQKVRNEVFAMMNRWFDKGIDGFRMDVINLIAKKPGLPDSDKKPN
                     AQGYVMADDCFANQPKVHDYLKEMRKTCFDGRDCMCVGETPFVTKQIAMDYVDPKNHE
                     LDMLFQFDLMDIDSGESGKWEVVPWKIADFKAIITGWQEALKDGWNSLYWSNHDQPRP
                     VSRFGSCTSEIYRVRSAKMLAALMYLLKGTPFIYQGEEIGMTNMPFSDISELRDIESI
                     NFYKLSEKGGDPKCAWESILIKGRDNSRTPMQWDSSENGGFTTGKSWIRINPNYRNIN
                     VRNAMEDDQSILHFYRSLIALRNSHDALIYGDYTEILHEHPRIFAYVRTYGAERWLVF
                     ANMSGENAFLDSSDFFENGKVVLSNMDEIQDLVLFQPYEVRVIRA"
     gene            complement(8030..9046)
                     /locus_tag="PK898_08140"
     CDS             complement(8030..9046)
                     /locus_tag="PK898_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU20038.1"
                     /translation="MKRVTIKDVANATGLSIATVSYVLNGKGNIPEETRQIVNEAVQK
                     LGYIPDYSARSLVSRKSNLIGVVIPQAEPGSTLMFQNPFYSEILSSIEYTARKNGYHV
                     MISGTNADEKYLKLAQQRNLDGIIIIGMYSHESYADLTKANIPLVLVDSYVDDHHFHT
                     IKINDRYGGYIATRYLIEHGHRKIAFLSGALRESSVNRQRLAGYYDALKEYGIPICKD
                     YVLTDQINFHAGMHLAQTVKNSTDVTAVFCTADVLALGVIKELNKNERVVPEQISVMG
                     FDNLEIAEFCVPGLTTVGQDIYQKGKTAVELVLNHIKNPNIGKQEISLPVSIVERESV
                     RTIS"
     gene            complement(9084..11045)
                     /locus_tag="PK898_08145"
     CDS             complement(9084..11045)
                     /locus_tag="PK898_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU20039.1"
                     /translation="MIRIHNANPDRIHNLRIIPQESGNLLTYESPSTAVYGMGERYDS
                     INRLNRKTVIRVRQKFTHQGEQTYFPLPFYLSDNGHGLYVRTDREVIFDLTPQEFRVE
                     ISDPDCDVFFFYGSPKEIVSAFIEMTGKAFLPPEWAFGPWVSANRWNKQSDIEEQREM
                     IRKYHYPATVLVIEAWSDEATFYIWNGARAPLKNGEDSFSMADFRFSEPWPDPQGMID
                     ALHAQGIRLLLWQIPALKKLDEGQISPQHDADVAFALANHLVAWHADIERTPYQIPEE
                     WFIGSYLPDFTNPKTREWWMAKRKYLTDMGVDGFKTDGGEFVYDPTIQFSNGKSGESM
                     ANRYCVEYEETYHHAIGNERVLFSRAGYTGAQAVSLHWAGDQCSTFDELKAVLNAGIS
                     LGLSGIPFWGFDIGGFAGPMPSAELYLRATALAVFSPIMQWHSEPMGGQYGGMAERDH
                     INDRSPWNMEKQSGDPNVCTTAVFFANLRMNLLPELYNQAWKASEKNQPMMRHLIFDY
                     PTDQAAVNCEDEFMLGDLLVAPIVEEGVIQRGVYLPEGGWYDFWSGKRYEGQSMIEVS
                     AALDRIPVFLREGGAVMLHLPAEGRLGDDVGNQVGNYDHLVLLTGGTAADYSFRDSTG
                     HSFRLSGGQSEGIIPDEITVKNLQDSPMI"
     gene            complement(11048..11872)
                     /locus_tag="PK898_08150"
     CDS             complement(11048..11872)
                     /locus_tag="PK898_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU20040.1"
                     /translation="MVRKRISKIGYYLVIILISFISMLPFLWMISTSFKSRGALMTLP
                     IEWIPKNPTFDAYIKVFTRFPFARAIGNSFFIAVSYTVLTILSSSMAAFAFAKIKFPF
                     ADGLFKVYLASMMIPVQVTLIPLFVIMNKLGLINTYTSVISPSLFRVFAIFLLVQTIK
                     SIPDDYLDAAKIDGAGLFTTYRQVVMPLCIPTIATLCITTFMDSWNDYLWPLVMLTDR
                     NYMTLPIALSTLNGQYATEYNVLMAGSLISMIPIIVVYIFAQKSFKGSLVSGGIKG"
     gene            complement(11862..12773)
                     /locus_tag="PK898_08155"
     CDS             complement(11862..12773)
                     /locus_tag="PK898_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU20041.1"
                     /translation="MEKRKTGLKNQMSAVPFLLPSFIGLIIFSLLPIVISFFLSLTDW
                     NGIDSLNLNVFLKDHFIGFQNYVKILTGGEFFRVLGNTLYFIVLYIPLMLIFSLLIAW
                     LMNKNYKGVSLFRILTYIPVLSSWIAASLIWKWVLAPKFGIANNVLSIFGISGPAWLQ
                     SEQWAMPSIVLVSVWKDTGFFGLILLSGLQGIDKTYYEAAAIDGANSWQQFWKITLPL
                     LSPSIFFVLIISLINSFQLFPQVMIMTDGGPNGATQVMVERIYKYGFKYYEMGYASAF
                     SWLLFVIIFALTNLQMKMQKNWVHYGA"
     gene            complement(12883..14118)
                     /locus_tag="PK898_08160"
     CDS             complement(12883..14118)
                     /locus_tag="PK898_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU20042.1"
                     /translation="MKKFVLIVLSVLLVLSLGAWQQADKNVTITYTNFSSSGGNEETL
                     QKMYDLFHAKNPDITVNIETIGYNDYFTQMQTRVAGGTAPDCFELNIENFAAYAAKGV
                     LKEITGVDLSGINPTALSAFNMDGKQYGLPGSFSNVVLIYNKDLFDKAGVAYPTIDWT
                     WEDAQKAAEAIKALGDNTFGIFAPITYNEFYKVSAQFGGSLFNEDKTAFTVNSPENIK
                     AVKNMVERVTVTHVQPNAEEQGGMGDWDWFMSGKLGMIPTGIWAFPTFAENCDFDWDI
                     EVEPGQVKKATHFFSNAVVINAKTTDEKAAAAAKWLTFLASDPDAAALRIQAGWELPA
                     ITDQNVLNTYLTAGKPANRKAVFESLDYLVLPPVIEQNALMADIVNKHLESAASGKVS
                     VEDALNAMQAELEEKIKLK"
BASE COUNT         3918 a         3586 c         2963 g         3905 t
ORIGIN      
        1 ttcatccgag acatctcttt caaaagtggt ctcttgactg attgatttgg cggcagcggg
       61 ttcgggttca accggactgt catcaattcc caaagcacga ttatgatatt ccgccgccga
      121 ttttcccaaa taaggtacta ataaagatac gggataagcg gcaagttgtc cgatcgtaga
      181 tatgcctaaa ttttcgaaaa tttgggctgt tttcttcccg acaccccaca gttcatccac
      241 cttcaaaggt gataaaaaat tggcttcttt tccgttcgga atccaaagaa tcgcattggg
      301 ataggtatcc gtgataaccc gtttttttcc gatgttatcg gcaattttag caaccagctt
      361 attggatgcg gctccgagcg aacagggcaa atgcggcttc tgattaattt ccttttgcac
      421 cgaccgcgca atctcctcga tcggttgcgg aagatctgta acatccagaa aggcttcatc
      481 aatcgatatc tgttcaacca acggcgtaaa atgctgaaga attgccatca cttcacggga
      541 tttctcacga taatactcaa atccgctgtg aacaatgatt aaatctggac agcgtcggat
      601 cgcttgggac atcggcattg cggaatgaat gccgcatttt ctggctgcat aggaacaggt
      661 ggagacgact cctcgtcgat ccggactgcc cccaaccgca aacttttttc cttttaaaga
      721 aggattgagc agctcttcga ccgcacagaa aaaagcatcc aaatccaagt gaagtatttt
      781 tcggtatttt gccatttgtt ctattataat gctcgaatag aacaagggga aaactatttt
      841 atcggttttt tcggaaaatt gcggcagccg aaataatcgc tgtctgcggc tcatcatcaa
      901 acgggatgat tgcattgagc ttatatccgg aatcgctcaa acggatcgtg tcacgtgcca
      961 gaatcgcact atcgtggctg atgtaaagaa tccgggaaac ttccgccttt gataaggctt
     1021 gagcggtcgc gacggatatc ccttccgagg ccgcttcgaa aacggtcagc ggtttatttt
     1081 ctctgaatgc cgggataatc gattcgggct ttccgagata aagatccacc gaatcaaaag
     1141 catccaaatt aaccacaaaa tcatccgcac tgcgttctcc gacatcctgc gcgatcactt
     1201 tccgcccctg tgaagcaaac catttcgccc aaaatccggt accgcaattc aaattcaaca
     1261 ccggcttatc ccgatcttcc ggaagaatct cgatcaattt ctgacacaaa gcgtcgtaaa
     1321 tttcgggatt ggggaaaaaa gggctgtctt ctgaggcgca gatttcctgc ccggcgattt
     1381 cttgaaccaa agtgctgttg ccggataaaa caaaagatcc gaccggaccg gaataaacca
     1441 gcgaaacgct caaatcggtt tcgatttcca tcggcggacg atccgcatca cctcttaaaa
     1501 caatctgtag ttcaccttcg acaccttctc gaaattcggc tgcatgaatc ccgctttcgg
     1561 cttcaaatga tatctgccgt atcaactcat tgattccctc actgcaaatc gggcaacgat
     1621 ccgcgacaaa cattccgccg gacacgtcct gtaatcccag tttcccgtcc ggcaaaacag
     1681 agaaaatcat gttctttcga aatccgtaat aaggatctga tggaatggtt ggctgaatca
     1741 atgattcggg atggcccatt ttcccgattc tcttcaactg atcgattaaa atattccgtt
     1801 ttacttccgt ttgatcggaa taattcagat gttgcagacg acagtgggcg caagttccga
     1861 aatatctgca tcgtgaagaa gtccgtttgg gggaataaga taaaatttct attaattccg
     1921 cctgcacgta ccgcttgtcc gattttgaat agcgaatcaa tacccgctca ccggggatcg
     1981 tgccggggac aaagacaacc cttccgtccg acaatttccc cacagcctta ccgtcgctgc
     2041 tgaaatttgt aattgttatt tcttctttgt tataagcggg gctcaatttt tcaccgttct
     2101 tttcattttt tcctaaatta taatgggaag tgatgaacgt aaaaaaattg atgcgggcaa
     2161 tcggttggga aattttcatc ttgctttgtg ggagtcttgc cggctgtcat cctgtttcaa
     2221 atcaggctgt cggttcggaa tcggtacacg ttaccttacc gcctccccaa caaattcagc
     2281 tgccttctct gactccgttt tccgccgtcg aaatttcgac ggtaactttc ctcccggaaa
     2341 atcggtcgaa gtcagcgacg ttgcctgctt ttttccaaac gccgacactg aaagttgttt
     2401 ctggaatcga accggattca aaagaatatg acgtcccgct tgtcactcag gacggcctaa
     2461 aatataaagg ggtaaaaact accctcggtt gtaccgcggc gtccgttcaa atggtcttgg
     2521 attattggaa aaaggtaaat ccggatttta agacgatcag tgcccaaaaa atcatcgata
     2581 taaatacttc tcagggtacc tttcgcaccg gtgaagggtt atctattgag aacgtcaaag
     2641 acgaattgca gaatatcggc tatatccttg aaatttatca aaacagcgac aaagagcagc
     2701 ttttaagcgt attcgatcaa ttcggcccgc agattttgct ggtcaagacc ggctgggttc
     2761 cgaccggagc caatcacttg gttgtcttgg tcgcctatga caaagaaacg gataccgtca
     2821 ccgtcaatga tccgtggttt gatcatcctt ttacttggga ctgggaagct tttgacggaa
     2881 tctggagtct taactattct caaaaagaaa acggctatct cacccgatct tttttcaaca
     2941 tccggcccgc atctttatcc gacaattcgg attaatagac ggcgataatc ccgcgattat
     3001 cgagagttgc ccaagatgtg aggaaggtat taatcgagcg tgtgtaccgc attgcaccat
     3061 cctgaatcgt cacattttgg ctgtcatatc cgatgacaac gacggtatgt tgatagggaa
     3121 ccactttggt agactttcca ttggaaggtg tgtaatactg tggtgtaccg acttccgtat
     3181 taccggctac ccataccatt acaggtcgcc cgtttgaaat ttcatatttt attcgttccc
     3241 aactcatgtt tttatatccg actgcgggaa ccccgtagct tctgaggagc gccgccacag
     3301 gttcttgata gacgccataa ctggcaggcg gaatttgtcc ccgtggatct gtgtaattcc
     3361 caacaaatcc ttcgttcgga tcatccgatt tcggcagact ggttaaaaat tggtaagcgt
     3421 cgattgaaaa accgaaataa gcggcaaaat caacagccga tcgtgtttcg caatccagtg
     3481 gataagattg ggcatgcccg acaaatccgt ccacataagc ttgggtaggc aaaggctgac
     3541 tataatccca tatgtcattc aaattgccaa acgaaccact gaacggcata aaggtattgt
     3601 tatcataatt gtaattatcc gcgctctcat cttcggggaa ccaaaaccat ccgacataat
     3661 cttccgaatt aggagaggaa ttccaatcaa aatcgggggt cacttccggg atcactccaa
     3721 actgatcgtt ctgatcctgc tctgtatcaa agctgaaata atcttccgtt tcggtcacgg
     3781 gatatttttg gatcgttatt tccggcagag gagtaggttc atccgcagtt tgttccgaat
     3841 cccaaaattc atcgccgggt tgaccccacc aatcggtttt gtcgcttgcc gttttatccg
     3901 gcatgatgat cagtgcccgg ttacccaaaa caccccatga cgcttcaaac gcttcataag
     3961 atcgggtata actttctccg ttatcccaga ttaaaagtcc cgactgatca aatcccgtta
     4021 cggttaccga attcatatga agggccaccg ttgttgattg tccgcttgaa ggtgtatatt
     4081 cgaccggcgt tccggcggcg gtatttccga ccacccaaca gattaccgga ttcccctgcc
     4141 ggacggatgt tttcagttgg tccaacgaat aattttgata accataagcc ggcactccca
     4201 acgtctgcag cgctttactc aaaggctgct gataaacccc atatgagttc ggcggaagtc
     4261 ctccttcatc atcgtaatac ccgacataac cggtattcgg atcatccgtt aatgggattt
     4321 tatccataat ttccaaaggt tcgatcgaat aaccatagaa acttgccaaa tcgctcgcac
     4381 attgcgcttc gctgatcaat ttatattgca tccggttact gacataattc gtaatcgatg
     4441 cggaatcgct ttgggcaaga actttcagcg gaatgctgag aaaaagacac agaaaaagga
     4501 gaataaatcg gttttttttc attaaaatat tgtctgcctt tggtttttat cgattaatac
     4561 cccgtttgag aacttacgat aatatcaatg aagaagtcct gaccaaacgg ttttccccgg
     4621 tcgtcataag cctgccactg actgtaatat tcccccgttt cttccggcgc atggaaaaca
     4681 atttcgacgg aacccgtctc accgggagcg atcggttcaa gcttttgctt cggttcggcg
     4741 cccatactgg caccggataa aattcgaacg gaatatttgg cggtccattc gcatgtaccc
     4801 gagttttcaa cctgccagcg cttaacaatc tccgcgccgg gttcaaaaac agtcccatcc
     4861 ggaacagtca agtcatcgat aaaagtcaga tcgttggtac aaggtccccg cggatcaatc
     4921 gtcggttgaa atgggtcggt ggtcggcgtt ttaaaagagg tggcaaccag cgtcggcgga
     4981 ataaattgac tgctgagcac accctcgctt tgcgaacgca agcacccggt cacaattgcc
     5041 gttgagagaa acatcaggga acagagaaat aagaatcttt tacgacgctg tctgatcatc
     5101 atcttttttg gtgcaatatt tatgccatcg attttaacat aaaaaaagcg ccgttattaa
     5161 agcagcggca cttttaaaaa cttttttatt cttcctcgga gctgtcctct tccgaatctg
     5221 aagaaaaaga taaaatcggg atatcgctcc ccaatgcggc attccggatt ttttcttcga
     5281 tctcatttgc caactccgga ttggctttca ggaaatcttt tgcattctcg cgcccctgac
     5341 ccaatcggat atcattataa gaatagaacg aaccccgttt ggtaatgatt tcgagttgtg
     5401 ttcccaaatc aacaatatcc ccggttcgag aaattccttc gttatacatg atatcgaatt
     5461 cggctgtcaa gaacggcggc gcgactttat tctttgtcac ccgtactctg acccggctgc
     5521 caaccacttc cgccccggat ttgatcgaat tgatccgacg aatatccagt cgcaccgaag
     5581 aataatattt caatgcctgc ccgccggtag tggtctccgg attgccgaac atcacaccga
     5641 ttttcaatcg taattggttg gtaaaaatga cagcggtttt ggtttgattg atcgcgccgg
     5701 aaagctttcg cagagcctga gacatcagtc gagcttgcac ccccatggag gaatccccca
     5761 tatccccttc gatttcggaa cggggaacaa gtgcagctac cgaatccaca accaccacat
     5821 ccaccgcgcc ggatcgtacc aatgtctcac agatctcgag cgcttgttcg ccggtgtccg
     5881 gttgagatac atataactcg ttgatattga cgccgagccg catcgcatat ttcggatcca
     5941 gcgcatgctc catatcgata aaagccgccg tcccgcccat tttctgcgct tccgcgataa
     6001 tatgcagaca tagggttgtt tttccggagg attccggacc gtatatctcg gtgatccggc
     6061 cgcgcggtat tccgccgaca cctaacgcca gatccagcga aatcgacccg gttgaaattg
     6121 cttccacttc catatgtttg gcatccccta atcgcataat cgacccttcc ccaaagcgct
     6181 tggaaatatc ggtcaatgct ttatccagca tcattctttt tgcgtccggg tcaatttcac
     6241 cgtaaggttg agagaattga tccgctgatc tttttggcat aatccctccg gtttgcgctg
     6301 aattttgttt gaattatagt acatttgttc aataaacgtc aaggattgcc ggtaaaactt
     6361 cccaaaaaat ctcaagcccg gatcacacga acttcataag gctggaacag caccagatct
     6421 tgaatttcat ccatattcga aagcacgact ttaccgttct caaaaaaatc cgaggaatcc
     6481 aaaaaagcat tttccccgct catattcgcg aaaaccaacc atctttctgc gccatatgtc
     6541 cgtacataag caaagattcg gggatgctca tgcagaattt cggtataatc gccgtaaatc
     6601 aacgcgtcat gactgttcct taatgcgatc aggctccgat aaaaatgaag gatcgattga
     6661 tcatcttcca tcgcatttcg cacattaatg tttcgatagt ttggattgat gcggatccaa
     6721 gatttgccgg ttgtgaatcc gccgttttcc gaagaatccc attgcatggg agttctggag
     6781 ttgtcccttc ccttaatcag gatactttcc caggcgcatt tcggatctcc gcctttttcg
     6841 gaaagtttat aaaaattgat gctttcgata tctctgagct cggaaatatc cgaaaagggc
     6901 atgttggtca ttccgatttc ctctccctga taaataaagg gagtcccctt caaaaggtac
     6961 attaatgcag ccagcatttt agcggatcga acccgatata tttccgaagt acaactgccg
     7021 aatcgcgaaa cgggccgcgg ttggtcatgg ttactccaat ataagctgtt ccacccgtct
     7081 ttcaacgctt cctgccaacc ggtgataatc gccttaaaat cagcgatttt ccacgggacg
     7141 acctcccatt ttccgctctc tccgctgtca atatccatca gatcaaattg gaaaagcata
     7201 tccaattcgt gatttttcgg atcgacataa tccatggcaa tctgtttcgt tacaaagggt
     7261 gtctcgccca cacacatgca atcccgaccg tcaaaacagg ttttccgcat ttcttttaaa
     7321 taatcatgaa ctttcggctg atttgcaaaa caatcgtccg ccatgacata tccttgagca
     7381 ttcggctttt tatcgctgtc aggtaatccg ggctttttgg cgatgagatt gattacatcc
     7441 atccgaaaac cgtcaattcc cttatcaaac caacggttca tcatggcaaa gacctcattt
     7501 ctcacctttt ggttctccca attcagatcc ggttgctttt cggaaaacaa atgtaaatac
     7561 cattcatctg tcgcggcatc atatttccaa gccgacggag tgaagaaaga cgcccagtta
     7621 ttcggttctt tcccgtttcg tcccggacgc caaatgtagt aatcccgata tggattattc
     7681 cggctttttc cggattcaac aaaccaagca tgctcatccg aagtatgatt cacgactaaa
     7741 tccatgatca gccgaatacc gcgtcgatgc atctcagata agagccgatc aaaatcggcc
     7801 atcgtaccga attcatccat aattttttca taattccgga tgtcataccc catatcatca
     7861 ttcggagaat cataaaccgg gttcagccag accgcaccga ctcccaacca cttcagatag
     7921 tccaacttct ccgttatccc gttcagatct ccgatcccgt ctccattact gtccttaaaa
     7981 ctacggggat aaatttgata aaacacgcat tctttccacc aagattttct catgatattg
     8041 tcctgaccga ttccctttcc acgatagaaa ccggaaggga gatctcttgt ttaccgatat
     8101 tcggattttt aatatgattc aaaaccaact ccacagccgt tttccctttt tggtaaatat
     8161 cctgtccgac agtggtcagc ccggggacgc aaaactcagc aatttccagg ttatcaaatc
     8221 ccatcacgga aatttgttcc ggaacaacac gctcattttt gttgagctct ttaatgacac
     8281 ccaaagccag tacatccgcc gtacagaaaa cagcggtaac gtccgtcgaa ttcttaacgg
     8341 tttgagcaag atgcataccg gcatgaaaat tgatctgatc ggtaagcaca taatctttgc
     8401 agatcgggat cccgtattct tttaatgcat cataataacc cgccagacgc tgccgattga
     8461 cactgctttc acgcaaagca ccggaaagaa aagcgatttt tcgatgccca tgttcgatca
     8521 aataacgagt ggcaatgtat ccgccgtatc gatcgttgat tttgattgta tgaaaatgat
     8581 gatcatccac gtaactgtca accagcacca acggaatatt tgctttggtt aaatccgcat
     8641 aggattcatg agagtacatc ccgatgatga tgatcccgtc cagattgcgt tgctgtgcca
     8701 gtttcaaata tttttcatcc gcattcgtac cggatatcat cacatgatag ccgtttttac
     8761 gggcagtata ttcaatgctt gacaaaattt cactgtagaa cggattttga aacatcagtg
     8821 tgctgccggg ttccgcttgc ggaataacca caccgatcaa attgctcttt cgacttacca
     8881 ggcttcgggc ggaataatcg gggatatatc ccaatttctg taccgcttca ttgacaattt
     8941 gccgcgtttc ctccggaata ttcccttttc cgtttaagac ataagagacc gttgcgatcg
     9001 acaacccggt tgcattcgca acatctttta tcgttacccg tttcattact ctgactatat
     9061 ccttggatta aacgtttcag agattatatc ataggcgaat cttgcagatt tttcacggtg
     9121 atttcgtcag gaatgatccc ttccgattga ccgccggata gccggaaaga atgaccggtg
     9181 ctgtcacgga aagagtaatc cgctgccgtc ccgccggtca gtaagaccag atgatcataa
     9241 tttccgacct gattacccac atcgtccccc aatcttcctt cagccggaag atgcagcatt
     9301 accgctccgc cttcccgtaa aaataccgga atacgatcca aagccgcgga aacttcgatc
     9361 atcgattgac cttcatatcg tttcccgctc caaaagtcat accatccccc ttccggcaag
     9421 tagacccctc tttgaataac gccttcttcg acaatcggag ctaccagaag atctcccagc
     9481 ataaattcat cctcacaatt taccgccgct tgatccgtcg ggtaatcaaa aatcagatgc
     9541 cgcatcatcg gttgattttt ttctgaagcc ttccaagcct gattatagag ttccggtaaa
     9601 agattcattc ggaggtttgc aaaaaaaacc gcagtggtac aaacattcgg atcgccgctt
     9661 tgcttttcca tgttccatgg actgcggtcg ttgatatggt ctctttccgc catcccgcca
     9721 tattgcccgc ccatcggctc actgtgccat tgcataatcg gagagaaaac agccaatgcg
     9781 gttgcgcgca aataaagttc cgcggatggc atgggtccgg caaagcctcc gatatcgaat
     9841 ccccagaacg gaattccgga aagtcccaac gaaataccgg cgttcaaaac cgcttttaat
     9901 tcgtcgaaag tcgaacattg atcccccgcc caatgaagcg acacggcttg tgcgccggta
     9961 taacctgccc ggctgaataa aacacgttcg tttccgatcg catgatgata cgtttcttca
    10021 tattcgacac aatagcggtt cgccatggat tctccggatt ttccgttgga aaattggatc
    10081 gtcgggtcat agacaaactc accgccgtcc gttttaaatc catcgacgcc catatccgtc
    10141 aggtattttc gtttcgccat ccaccattca cgagttttcg gattggtaaa atcgggtaaa
    10201 taggaaccga tgaaccattc ctcgggaatt tggtaagggg ttcgttcaat atccgcatgc
    10261 caagccacca aatgattcgc aagggcaaaa gcgacatcgg catcatgctg aggactgatt
    10321 tggccttcat ccaatttttt caatgccgga atttgccata aaagcaatcg aatcccctgc
    10381 gcatgcaacg catcgatcat tccctgcgga tccggccacg gttcagaaaa acggaaatct
    10441 gccattgaaa acgaatcttc gccgtttttc aaaggagccc gagcgccatt ccagatataa
    10501 aaagtcgctt cgtcactcca agcttcgata accagaaccg tggcgggata atgatatttt
    10561 ctgatcatct cccgttgttc ttcgatatcg ctttgtttgt tccaacggtt tgccgatacc
    10621 caaggtccga acgcccattc cggcggtaaa aaggcttttc cggtcatctc aataaatgcg
    10681 gaaacgatct ccttcggaga tccgtagaaa aaaaacacgt cacaatccgg atccgatatt
    10741 tcaaccctga attcttgagg agtcaggtca aaaataactt ctcgatctgt tcggacgtac
    10801 aagccatgcc cgttatccga taaataaaat ggcagcggga aataggtttg ttccccctga
    10861 tgtgtgaatt tctgtcgaac ccggatcacg gttttccgat tgagacggtt tattgaatca
    10921 tagcgctcac ccataccgta aaccgctgtg gaagggcttt cataggtgag aagattcccc
    10981 gattcctgag gaatgattcg aagattatgg atccgatccg gattggcgtt atgtattctg
    11041 atcatattta tccttttatt cctccggaaa caaggctgcc tttgaaactt ttttgagcga
    11101 aaatataaac cacaatgatc gggatcatcg aaatcaggct gcctgccatc aaaacattgt
    11161 attcggtggc atattgtccg ttcagtgtgc ttaaggcaat cggcaatgtc atataattcc
    11221 tatcggtcaa cataactaac ggccagaggt aatcattcca tgaatccata aaagtcgtga
    11281 tgcacaaggt cgcgatcgtc gggatacaca gcggcatcac gacctgacga taggtcgtaa
    11341 acagaccggc accgtctatt ttcgcggcgt ccaaataatc gtccggaatc gacttgatcg
    11401 tttgaaccaa tagaaaaatg gcaaatacac gaaacagcga aggtgaaata acgctggtat
    11461 aggtgttgat taatccgagt ttgttcataa tcacaaataa gggaattaat gtgacctgta
    11521 ccgggatcat cattgaagcc agatagactt taaaaagtcc gtcggcgaat gggaatttga
    11581 ttttggcaaa agcaaatgca gccattgaag aggaaaggat ggtcaaaacg gtgtatgaaa
    11641 cagcaataaa aaaactgttc ccgattgctc ttgcgaacgg aaagcgtgta aacactttga
    11701 tatatgcgtc gaaagtcgga tttttcggaa tccattcaat cggtaaggtc atcaaagcgc
    11761 cgcgactttt gaatgaggtt gaaatcatcc atagaaacgg caacattgaa atgaaggaaa
    11821 tcaagatgat tacgagataa tatccgatct tcgatatccg tttacgcacc ataatgcacc
    11881 cagtttttct gcatcttcat ttgcagattc gtcagagcaa aaataatcac aaaaagcagc
    11941 caagagaatg ccgatgcata acccatttca taatatttga acccatattt ataaattctc
    12001 tcgaccatca cctgtgtcgc accgttcggt ccgccgtccg tcataatcat gacttgcggg
    12061 aagagttgaa aagaattgat caaactgatt atcagaacaa agaaaatcga aggactcagc
    12121 aatggaagtg tgatcttcca aaactgctgc cagctattag caccgtcgat tgcggcggct
    12181 tcataatacg ttttatcaat tccttgcaaa ccggaaagca aaatcagccc aaaaaatcca
    12241 gtatctttcc aaaccgaaac caatacaata gaaggcatcg cccattgctc gctttgtaac
    12301 caagccggtc cggatattcc gaatatcgaa agcacgttat tggcaatccc gaatttggga
    12361 gccaataccc acttccagat cagcgaagct gcgatccatg aactcaaaac gggaatatag
    12421 gtcaaaatac gaaaaaggct cacccctttg taatttttat tcatcaacca agcaataagc
    12481 aatgaaaaaa tcaacattag cggaatatat aaaacaataa aataaagggt atttcccaaa
    12541 acccggaaga attctcctcc ggtcaaaatt ttgacatagt tttggaagcc gatgaaatga
    12601 tctttcaaaa aaacgttcaa gtttagacta tcgatcccgt tccaatccgt aaggcttaaa
    12661 aagaatgaga ttacgatagg cagaagcgaa aagattatca ggccgataaa actgggcaga
    12721 agaaatggaa cagcggacat ttgatttttc aatccggttt ttctcttctc catgacacct
    12781 cccctttctt ttcgattgat ttgtttcctt caaaccgaga ctcgcccggt ttacgggtga
    12841 gcggcggttt ggtcgcccac ccgtataaac agattgattt gattatttca gttttatttt
    12901 ttcttccagt tcagcctgca tagcgttcaa tgcatcctcg acggaaactt tgccggaagc
    12961 ggcggattcc aaatgtttat tgacgatatc cgccatcaaa gcattttgtt cgatcaccgg
    13021 cggcagcacc agataatcca acgattcgaa caccgctttc cgattagccg gctttccggc
    13081 tgtcaaataa gtgttcaata cattttgatc ggtaatcgcg ggcaattccc aaccggcttg
    13141 aatgcgcagc gcggctgcat ccggatccga agccaaaaat gtcagccact ttgccgcggc
    13201 ggcagctttt tcatccgtcg ttttggcatt aatcacaacg gcgttggaga agaaatgggt
    13261 ggctttcttc acctgaccgg gttcaacctc gatatcccag tcaaaatcgc agttttccgc
    13321 aaaagtgggg aaagcccaaa tgccggtggg gatcattccc aattttccgc tcataaacca
    13381 gtcccagtca cccattccgc cctgctcttc cgcattgggc tggacatgag tcacagtcac
    13441 ccgttccacc atatttttga cggctttaat attttcgggt gaattaaccg tgaacgccgt
    13501 cttatcttcg ttgaacagac ttccgccgaa ttgagccgaa actttataga attcattata
    13561 ggtaatcggt gcaaaaatac caaaggtatt atcgcctaac gctttaatcg cttcggcagc
    13621 cttctgagca tcctcccagg tccaatcgat cgtcgggtat gcaacaccgg ccttatcaaa
    13681 aaggtctttg ttatagatca aaaccacgtt tgaaaaagaa cccggcaagc cgtattgctt
    13741 cccgtccata ttgaatgcgg atagcgccgt cggattaatc ccggacaaat cgacacccgt
    13801 gatttccttc aacacccctt ttgcggcata agccgcgaaa ttttcaatat tcagctcaaa
    13861 acaatccggt gccgttccgc cggcaactcg ggtctgcatc tgagtgaaat aatcgttata
    13921 accgattgtt tcgatattaa cggtaatatc cggattcttt gcatggaaaa gatcgtacat
    13981 cttctgcaaa gtctcttcat ttccgcccga tgagctgaaa ttcgtatatg ttatcgtaac
    14041 gttcttatcc gcctgttgcc atgcacccag gctcaacacc aataaaacag acaaaacaat
    14101 caaaacgaac tttttcattt tccctcctaa aaatgaaatg agcaaaagca accggctctg
    14161 ttaaacgttt cataaaacaa atcggttgct ttcctcatat tttgtgtgat attttgcaat
    14221 ttcttgccaa atattatttt attttcagcc atacaggctc agaactactg aaacgtttaa
    14281 ctcatttaat tagagtatat cgcgtctttt acaattgtca atacccaaaa aaagagaaca
    14341 aagattgatt cgtcgataat tcacgtaggg gc
//
LOCUS       DAOSMM010000058        13768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4479_length_13768_cov_17.788449, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000058 DAOSMM010000000
VERSION     DAOSMM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13768
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4479_length_13768_cov_17.788449"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1236)
                     /locus_tag="PK898_08165"
     CDS             complement(<1..>1236)
                     /locus_tag="PK898_08165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU20043.1"
                     /translation="EMNPRFILGYAEGIRIQPSPSWVQRRLSAAGMRPINSVVDATNY
                     VMLELGQPLHSFDYDALVKRNHGQAPAIVTRHAYNGEKLMTLDKTERDLDESMMLVTD
                     QAGALSIGGVMGGLESEIVPETTRVLLESAAWNFINIRKTSSKLRLNTEAGYRNSRGV
                     HPAIAEEAARLCLAYIVEWSGGQVASGLIDNYALPVKDSVNHLSENDIVRLLGVEIPL
                     ATAAEYLSRLEFRCRLSDDGTTLEVTTPSHRLDIGEGVIGKADVLEEIARLFGYEQIP
                     TTRMADELPPAYVNRMVSFEEKLRDELASAGLQEVVNYRFTSPEREAKLLGKPAEKYV
                     TLANPISPDRSVMRTNLNVGMMEVVEKNIRLADTLEFFEIGPVFLPVEGEKRPHEEIH
                     LSVAITGKRIKDSWDTHYKD"
     assembly_gap    1237..1336
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1337..2117)
                     /locus_tag="PK898_08170"
     CDS             complement(<1337..2117)
                     /locus_tag="PK898_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20044.1"
                     /translation="MNIPLSWLKDFVDVDLPLEELARVMTMAGLEVDEIHLMGLPGPE
                     DDRHGFKFTGLSWPEDKFVVAEIREVNKHPDADKLVLCRLEDGTGEHTILTGAPNLYP
                     YVGKGRLEQPIKVAYAREGAILYDGHKPGFELTKLKKTKIRGFETKSMVCSAKELGIS
                     EEHEGIIILEPDAPTGVPLARYMGDAVFVIDILPNMARNACIKGVAREVAAQLNLPLK
                     TPKRTVPPEGRPIDGLASVRIEDPEMNPRFILGYAEGIRIQP"
     gene            complement(2117..3157)
                     /gene="pheS"
                     /locus_tag="PK898_08175"
     CDS             complement(2117..3157)
                     /gene="pheS"
                     /locus_tag="PK898_08175"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOU20045.1"
                     /translation="MAENDLLRQLESFERDSLAALEKITDEADLTKWRVANLGKSSFV
                     MQVFSKLGEAPKEDRPKIGQAANQVRERLETALAEKTEFLNRKSLEQKLTQERLDISL
                     PGRQPRKGRLHPANQNMREILAVLSGMGFEIFRSPEIETDEYNFELLNIPAYHPARDM
                     WDTFYTTDPGVLLRTHTSPGQIHAMRTFAPEPIRVALPGMCFRYEQVSARSEVQFNQI
                     ELLAVGKHITFGDLKGTLQDFAHRMFGENVRTRFRPSYFPFTEPSAEMDVECFLCGGK
                     GCSMCKNSGWLEILGCGMVHPTVLKNGGYDPEIYTGYAAGLGPERITILRHNVEDIRY
                     FWGNDIRFLEQF"
     misc_binding    complement(3198..3438)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3601..4059
                     /locus_tag="PK898_08180"
     CDS             3601..4059
                     /locus_tag="PK898_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU20046.1"
                     /translation="METEKEQIIEKIKILSLQKRNAVFRIAAANGLYPGQLPILEYII
                     DNEGCTQKEIAKKMRVSPASIASSTKRLQRSGLIQKKVDEGNLRQNMLFVTEKGRELA
                     ENCRNELSLFQDAFFDGFSEEELTRLSDILDHINRNISQGAKYHSKSFNH"
     gene            4099..5832
                     /locus_tag="PK898_08185"
     CDS             4099..5832
                     /locus_tag="PK898_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20047.1"
                     /translation="MSLKKLFPYIGQFKKQSRLAPLFVALEVVFDILIPFMMSKIVDV
                     GIASQDVGYIIRMCLLMVGAALLALVCGTLGTRFATLSATGFAMNLRRALFSKIQDFS
                     FYNIDKFSTASLTTRLTTDIQFIQIMYQMLLRMFIRAPLMMICATFMALTINKELAVV
                     FLVAIPVLAFFFAFITLKAHPRFVKMLAKVDSLNKSVQENLIAIRVVKAFVQRDYENA
                     KFESAALDLQNAQRQAEKIVILNNPIMTLTMDACIIAILWFGGNKIIIGEMMPGALFS
                     FLTYTTQIITALMLVSMVFTMFVISQASADRIVEVLEEKIDLTDDNGNPDLRVKEGEI
                     EFRDVNFSYAKSPDNLTLCDINLHIKPGEMIGIIGGTGSAKTTLVQLIPRLYDVLSGQ
                     VLVSGHDVREYKLNHLRDAVAMVLQKNVLFSGTIADNLRWGNENATDEELVRVCKVAQ
                     AHDFIMSFPDGYQTVLGQGGVNVSGGQKQRLCIARALLKNPKIIILDDSTSAIDTATE
                     LRFRKAFRENYRETTILMIAQRVQSIKDSDRILVMNEGKIDAFGTHDELIETNTIYRE
                     VFESQQKGVAL"
     gene            5829..7760
                     /locus_tag="PK898_08190"
     CDS             5829..7760
                     /locus_tag="PK898_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20048.1"
                     /translation="MNSENKNQNNLTQQTPGPGRDRQRLERPKDIWGTLIRILKTMSD
                     YKLQLGIVAVLMAVSSLSSVLGTYMLRPIINDYIIPLIAQSSPDLSGLIGMLLKMAGI
                     YSAGVIGNYTASRLMVNISSGTLRKIRVGMFEHIQNLPIKYFDTRTHGEIMSHFTNDA
                     DTLREMISNSIPNLISSAIMITGVIVMMIILSWQLFLLVILQLILIFFTIKFIGSRSS
                     SYYIKQQRELGSLNGYIEEMIEGQKVVKVFTHENKVKKIFNQKSEDLFKVASLANMFS
                     LVIQPIFGSYSYIHYALTAAVGTIMAIHGMLDIGTIGAFLQYTRTFTQPINQMSQQIN
                     AILAALAGAERIFILMDEPVEADEGNIVLVNVEKDHNEVIVESPVHTGYWAWKDVDEK
                     GTVTYTPLKGDMRFENVSFAYEPGNPVLKNISLYAKPGQKIAFVGSTGAGKTTITNLI
                     NRFYDVNEGKITYDGIDIKRIKKDDLRRSLSMVLQDTHLFTDTVMENIRYGNRNATDE
                     QVIHAAKLANADFFITHLPNGYQTVLYDDGENLSQGQRQLLAIARAAVKDPPVLILDE
                     ATSSIDTRTEALIEKGMDQLMEGRTVFVIAHRLSTVRNSNAILVLEHGEIIERGDHED
                     LLAQKGRYYQLYTGMFELS"
     gene            7997..9904
                     /locus_tag="PK898_08195"
     CDS             7997..9904
                     /locus_tag="PK898_08195"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOU20049.1"
                     /translation="MNYGFIKAAACTPEIVVADCIHNAQAILDLMKKSAAEGVRLAVF
                     PELCLTGYTCGDLFTQQTLLESVKQNLLKLAEESRSLPMISVIGAPIAHYGKLYNCGV
                     VLYGGKILGIVPKTEIPNYAEFYEARNFCAAPETNGTVRIGEDEIPFGKRLLFCCNEM
                     PEFIFGIEICEDLWVCQPPSTGHAIAGATLILNLSASNEIVGKADFRRQLVESQSARL
                     VCGYLYADAGEGESTTDLVFSGHNLIAQNGRILAEAQPFSSGYCVTELDLEELTSERQ
                     RLTTFPRQDATGYQMIPFNMEKTPVQLTRNISKNPFVPADSADLRDRCEKILAIQSAG
                     LRKRINHTHVKTVVIGVSGGLDSTLALLVSVRAMKESGHLPSDVIAVTMPGFGTTRRT
                     KNNAIKLCESLGVTLRKIDITRSVRSHFRDLGQDENDHNVLFENAQARERTQILMDIC
                     HQTDGFVVGTGDLSELALGWATYNGDHMSMYGVNASVPKTLVRYLVRYYADYLADKQV
                     SEILSDILNTPVSPELLPAAEGKISQLTENIIGPYELHDFFLYYFIRHGFSPEKVAWL
                     ANYAFKDTYSADEIQKWLKVFIKRFFNNQFKRSCLPDGPKVGSVSLSPRGDWRMPSDA
                     SFKIWIDSLDR"
     gene            9923..11185
                     /locus_tag="PK898_08200"
     CDS             9923..11185
                     /locus_tag="PK898_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20050.1"
                     /translation="MTIDSCNRIRKKIVKIVLPILFCTVCSGCYLIETIQPTPLPQKT
                     PTSLININALPTLPTLPPAVETATPIPESDFTYDTTGIIFDHLTEEPVPTEPNQAARV
                     IGTVSEFSGSDLIIKNSSGDSYTITCDDFCFYVDNRKKIISGEAVRPGAEIAVFGATD
                     SEKPEKIHADAIILNGTVKSDKTPKADMSYLPNGMTYQEFELASPPLPAPLRLNPLEG
                     SLDDHLNKRKMTTLSERTNFSYGFYGETYSTSLEYDTEGNRDPYYPTRSNLTIYSNGY
                     EFYSFWFPHVESPFFYNWGIVNYGGDWYLPIRMTVDINPDPDITELIYSDRTIMSQMN
                     FDKSFNYIRSFGFSIIGYNLFYFYQRPGGYGIAINRVDYPLNFDEIIFGLVGDYQDLN
                     PFYSDTLITFFGLRGNTWYYVELTAPEE"
     gene            11366..12787
                     /locus_tag="PK898_08205"
     CDS             11366..12787
                     /locus_tag="PK898_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOU20051.1"
                     /translation="MSLKIKFGTDGWRAYIAEDYTFDNCRRCAQGFANYLLKKDKKGE
                     CVVVGYDKRFHSENFAAACAEVMAGNGFNVLLTQEATPTPVISYAVVDKKAIGAINIT
                     ASHNPPSDNGFKVRDATGGAIDPQGLLEIEAGIPDDMSDVKRIPLNDGLESGRIEFFD
                     ASVNYISHLKDLIDLEPIRKSGLKILVDPMWGNGSGWFKRLLGGDKTEIIEIHNERNP
                     IFPEMKRPEPIPPNIDVALKKTVELGADVLLITDGDADRCGLGDENGVFLNQLQVMAL
                     LTLYLLEIRKERGAIVKTLSTTTMLNKLAKIYGVPIYETGVGFKYVAPKFKETDAIIG
                     GEESGGYAFRGNVPERDGILANLYFLDFMVKTGKTPSQLLQWLFEIVGPHYYERIDTS
                     FKGNAEEKKKFILDHIPEKLGGIKVTGLDTTDGFKFMMEDGGWMLIRFSGTEPIIRVY
                     TETTHEDCVDAILKDGLKVAGIE"
     gene            12780..13568
                     /locus_tag="PK898_08210"
     CDS             12780..13568
                     /locus_tag="PK898_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU20052.1"
                     /translation="MNNTDYWIDTHCHLNLPDFLPDQAAVIDRARQANVRRIIVPGID
                     PESSETAKKMAEAYPAVFFAVGVHPNESADWDDSRLQDLKILATHPKCVAVGEIGLDF
                     YREFCPPAQQLRSLHAQLELAEEIGKPVILHCRQAFEVLWPVLQDWHRHHSKNIGVLH
                     AFEESPEAAAEVTAQGFYVGIGGAYTYKKKKHLSEVLKTVSLDKILLETDSPYLTPTP
                     YRGQRNEPMYIPLIAEKVASTRNISTEALSEIVRENTFRLFGNL"
BASE COUNT         3755 a         3144 c         3076 g         3693 t
ORIGIN      
        1 atctttatag tgagtatccc aagaatcttt tatacgtttt ccggtaatcg cgaccgataa
       61 atgaatctct tcatgaggtc ttttctctcc ttcgacgggc aaaaagaccg gaccgatctc
      121 gaaaaactcc aaggtgtccg ccagacggat gtttttctca accacttcca tcattccgac
      181 atttagattc gtccgcatca cagatcgatc gggagaaatc ggattcgcca gtgtcacata
      241 tttttccgca ggcttaccaa gcaacttcgc ttcccgttcg ggagaagtaa aacgataatt
      301 tacaacttcc tgaagcccgg cggaggctaa ttcatcccgt aatttttctt caaacgaaac
      361 catacgattg acataggcgg gaggtaattc atccgccatt cgagtcgtcg ggatttgctc
      421 atatccgaaa agacgggcaa tctcttccaa aacatccgct ttaccgatga caccttctcc
      481 gatatccaac cggtgagagg gcgttgtcac ctccagcgtc gtcccgtcgt ccgataatcg
      541 gcaccgaaac tcaagtcgag ataaatattc cgccgcggtc gctaacggaa tctcaacgcc
      601 gagcaaacgg acaatatcat tttcgcttaa atgatttacc gaatccttca cgggcaaagc
      661 ataattatca attaaaccgg aagcgacctg acctccgctc cactcgacaa tatacgccag
      721 acataatcga gccgcttcct ccgcgatagc cggatgaacg ccgcggctgt tgcggtaacc
      781 ggcttcggtg ttcaatcgaa gtttagagga tgtcttccga atattgataa aattccaggc
      841 agccgattcc aataaaacac gcgtggtttc cggcacaatc tcgctttcca gaccacccat
      901 gacaccgccg attgaaaggg cgccggcttg atcggtgacg agcatcatgc tttcatccaa
      961 atctcgttcc gttttatcca acgtcatcag tttttcgccg ttataagcat gtctggtgac
     1021 aatcgccgga gcttggccat gattccgttt taccaaggcg tcatagtcga acgaatgcag
     1081 cggttgtccc aattccagca tcacatagtt ggttgcatcc accacactgt tgatcggacg
     1141 catcccggct gcgctgagac gacgctgaac ccatgacgga ctgggttgaa tccgaattcc
     1201 ttccgcatat cccaagataa aacgcggatt catttcnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnntggg ttgaatccga attccttccg catatcccaa gataaaacgc
     1381 ggattcattt cgggatcttc aattcggacc gaagcaagcc cgtcaatcgg ccgtccttcc
     1441 ggcgggacag tccgtttggg agtcttcagg ggaagattga gttgtgccgc cacttctcgc
     1501 gcgacacctt tgatgcaggc attccgcgcc atattcggca aaatatcgat cacaaaaacc
     1561 gcatctccca tgtaccgggc caagggaact ccggtggggg catccggttc gaggataatg
     1621 attccttcat gctcttcgga tatgcctaat tcttttgcgg aacaaaccat tgattttgtt
     1681 tcaaatccgc gaattttcgt tttcttaagc ttggtcagtt caaatcccgg tttatgtcca
     1741 tcataaagaa tcgctccttc gcgagcataa gcgactttga tgggctgttc cagccggcct
     1801 tttccgacat aaggatataa gttgggagcc cccgtcaata tcgtatgttc ccctgtccca
     1861 tcttccaacc ggcacaaaac cagtttatcc gcatcggggt gcttattgac ttcccgaatc
     1921 tcggccacca caaatttatc ttccggccag gataatccgg taaatttaaa accgtgacga
     1981 tcgtcctcgg gccccggtaa acccatcaga tgaatttcat ccacctcaag ccctgccatc
     2041 gtcataaccc gtgccagttc ttccaaagga agatccacat caacaaaatc ttttaaccaa
     2101 gaaagaggta tattcatcag aattgctcca ggaatcgaat atcatttccc cagaaataac
     2161 ggatatcttc gacattatgc cgaaggatcg taattctttc ggggcccaat ccggctgcat
     2221 aaccggtata gatttccgga tcataaccgc cgttcttcaa gacggtcgga tgtaccatgc
     2281 cgcagcccaa aatctccagc caaccggaat ttttacacat cgagcagcct tttcctccgc
     2341 agaggaaaca ttcgacatcc atctcggcac tgggctcggt aaacgggaaa taagaagggc
     2401 ggaatcgggt ccggacattc tctccgaaca tacgatgagc gaaatcctgc agggttccct
     2461 ttaagtcgcc gaaagtgata tgtttcccga ccgccaacag ctcgatctga ttgaactgga
     2521 cttccgatcg ggcgcttacc tgttcatagc gaaaacacat ccccggcaac gctactcgga
     2581 tcggttccgg cgcaaaagtt cgcatggcat ggatttgtcc cggagaagta tgcgttcgca
     2641 gcaaaactcc cggatcggtc gtgtagaagg tatcccacat gtctctggcg ggatgatatg
     2701 ccggaatatt caacaactcg aaattgtatt catcggtctc aatctccggc gaacgaaaga
     2761 tttcgaatcc cattccggaa agaaccgcta aaatttcacg catattctga ttggcgggat
     2821 gcaatcttcc ctttcgcggc tgacgcccgg gcagggatat atccaaacgt tcctgcgtga
     2881 gtttttgttc aaggcttttg cgatttaaaa attccgtttt ttccgccaat gcggtttcta
     2941 atctttctcg aacctgattg gctgcctgtc cgatcttcgg ccgatcttcc ttgggtgctt
     3001 ctcccaattt gctgaaaact tgcatgacaa aggaagattt tcccaaattt gccactcgcc
     3061 acttggtcag atcggcttcg tcggtaatct tttccagcgc agccaaagaa tctctttcaa
     3121 aagactccaa ttgccgcaat aaatcatttt ccgccatagt tcctctcaag atctaaaaat
     3181 aaaaactccc gttcctaatc agggacggga gaagtccgcg gtaccaccct tattgattga
     3241 tatccgatta tcaatccgct cgtttcccgt taacgccggg cctgcgttcc gaactactga
     3301 atccgtaaaa aaggatcgtt cccccggaaa gctctggcgt gaacttcaat cttcttcctt
     3361 ccgtgaaaca gtctcagtct ttgctgtttc ttccctgtcg gtttccgaaa atctactttt
     3421 cgccttcatc gcatttctta aacttttctt attataaatg caaaaaaaga catcgtgcct
     3481 cgttttttca caaaagtgat gaatatctct gtgtcacttt tttagcgcga ttctttttca
     3541 agatacacta taataagtaa tcgattaatt gttgcacttt atataacagt aaagaaaatt
     3601 atggaaaccg aaaaagaaca aatcatcgaa aaaatcaaaa tcctttcgtt acaaaaaaga
     3661 aatgccgttt ttcgaatcgc cgccgcaaac ggtctctatc cgggacaact cccgatattg
     3721 gaatatatca tcgataacga gggatgcacc caaaaagaaa tcgccaaaaa aatgagagtg
     3781 agtccggctt cgattgcatc ttcaaccaaa agattacaac gatccggatt aattcaaaag
     3841 aaagtggacg aggggaattt acgtcaaaac atgctatttg taaccgaaaa agggcgcgaa
     3901 ttggctgaaa attgccgaaa tgaattaagc ctttttcagg atgctttttt cgacggtttc
     3961 tcggaggagg aattaactcg actttccgat attctggatc acattaaccg gaatatttca
     4021 caaggcgcaa agtatcattc caaatctttt aatcattaaa gaatcgttct ttatcggaca
     4081 ttattcgaag gggaatcttt gtcactaaaa aaattatttc cttacattgg gcagtttaaa
     4141 aaacaatcca ggctcgcccc tctttttgtc gctttagaag ttgtttttga cattttgata
     4201 ccgtttatga tgtcaaaaat tgttgatgtc ggtatagcca gtcaagatgt cggctatatc
     4261 atccggatgt gcttattaat ggtgggagcc gcgttgctcg cactcgtctg cggcacattg
     4321 ggaacgcggt tcgcaacatt aagtgctacc ggctttgcca tgaacttgcg aagagcttta
     4381 ttttccaaga ttcaggattt ttcgttttac aacattgata aattttcgac cgcttcttta
     4441 acaacccggt tgacgacgga tattcagttt attcagatca tgtatcaaat gcttcttcgc
     4501 atgttcatcc gggctccgtt gatgatgatc tgcgccactt ttatggcgct tacaatcaac
     4561 aaggaattgg cggttgtttt cttggtggcg attccggtat tggcattttt cttcgcattt
     4621 atcactctca aagcgcatcc gcgttttgta aaaatgcttg ccaaagtgga cagtctcaat
     4681 aaatccgtcc aggaaaatct gatcgctatt cgggttgtta aagcttttgt tcaacgggat
     4741 tatgaaaatg ccaaattcga atcagcggcg cttgatcttc agaatgctca gagacaagcg
     4801 gaaaaaatcg ttattctcaa caaccccatt atgaccctaa cgatggatgc ctgtattatc
     4861 gctattctct ggttcggtgg aaataagatc atcatcggtg agatgatgcc gggtgcattg
     4921 ttcagctttc taacctatac cactcaaatt atcaccgctt taatgttggt ttcaatggtt
     4981 ttcacgatgt tcgttatttc tcaagcctcc gccgaccgga ttgttgaagt gttagaagaa
     5041 aaaatcgacc tgacggatga taacggcaat ccggatctgc gagtcaagga aggtgaaatt
     5101 gagttccggg atgttaactt cagttatgcc aagagtccgg ataatctgac tttatgtgac
     5161 attaaccttc atatcaaacc gggggagatg atcggaatta tcggagggac gggctcagca
     5221 aaaacgacct tggtacaact tatcccgagg ttatatgatg ttctctccgg gcaggtgctt
     5281 gtcagcggcc atgatgttcg cgaatataaa ctgaatcacc tgcgggatgc ggtcgctatg
     5341 gtattacaga aaaatgttct tttctccgga accattgcgg ataatttacg ctggggaaat
     5401 gaaaacgcta ccgatgaaga actggttaga gtctgtaagg tcgctcaggc gcatgatttt
     5461 attatgagct ttccggacgg atatcaaacc gtgctcggtc agggaggagt gaatgtttcc
     5521 ggcgggcaaa agcaacggct ctgtattgcc cgcgcattat tgaaaaatcc aaaaatcatt
     5581 attttggatg acagtaccag cgcgatcgat acggctacgg aactgcgttt ccgcaaagcc
     5641 ttcagagaaa attatcggga aacaacgatt ctgatgatcg cccaacgcgt tcaatccata
     5701 aaagattcag atcggatttt ggtcatgaat gaagggaaaa tcgatgcctt cggcacccat
     5761 gatgaactga ttgagaccaa tacgatttac cgggaagttt tcgaatccca acagaaagga
     5821 gtcgcattat gaactccgaa aataaaaatc aaaacaacct cacccagcag actcccggac
     5881 ccgggcgaga tcgacagaga ctcgaaaggc cgaaagacat ttgggggact ttaatccgaa
     5941 ttctaaagac catgtcggat tataaattgc agctggggat agtcgccgtt ctgatggcag
     6001 tcagttctct ctcatcggtt ctcggtactt atatgctgcg cccgatcata aacgattata
     6061 ttatcccgtt gatcgctcaa tcctctccgg atctgtcggg tttaatcgga atgctgttga
     6121 aaatggcggg aatctattca gccggcgtta tcggcaacta tacggccagt cgactgatgg
     6181 tgaatatcag ttccggtacc ctgcggaaaa ttcgcgtggg aatgttcgaa catattcaaa
     6241 atcttcccat caaatatttc gatacccgaa ctcacggtga gattatgagc catttcacca
     6301 acgacgccga tactctgcgc gaaatgattt caaacagtat cccgaatttg atttcctcgg
     6361 cgattatgat caccggcgtg attgttatga tgattattct gagctggcaa ctttttctgt
     6421 tggttatttt gcagctgatc ttaatcttct tcacgattaa atttatcggc agccgaagct
     6481 caagttacta cattaaacaa caacgcgaat tggggtcgct caacggttac attgaagaaa
     6541 tgatcgaagg gcagaaagtt gtcaaagtat ttacccacga aaacaaagtc aagaagattt
     6601 tcaatcaaaa aagcgaggac ctttttaagg tggcatctct cgccaatatg ttttctttgg
     6661 tgattcagcc tattttcgga agttattctt acattcatta tgcattaacc gccgccgtcg
     6721 gaacaattat ggcaatccat gggatgctcg atatcggaac aatcggcgcc tttttacaat
     6781 acacccgaac gtttacccag ccgattaacc agatgtcaca gcaaatcaat gcgattctgg
     6841 cggcacttgc cggcgccgag cgaattttca tcctaatgga tgagccggtg gaagcggatg
     6901 aaggcaacat tgtcctggtt aacgtagaga aagatcataa cgaggtcatt gtggaatctc
     6961 cggttcatac cggatattgg gcttggaaag atgtcgatga aaaaggtacg gttacctata
     7021 ctccgctgaa aggggatatg cgtttcgaaa atgtctcctt cgcttatgaa ccgggaaatc
     7081 cggtattgaa aaacatcagc ctttatgcaa agcccgggca aaaaattgcc tttgtcggat
     7141 cgaccggtgc cggaaaaaca accatcacca atttgatcaa ccgattttat gatgtaaacg
     7201 aaggaaaaat cacctacgac ggaatcgata tcaagcggat taagaaagat gacctgcgcc
     7261 gttcactttc catggtttta caggatacac atctctttac cgataccgtt atggaaaata
     7321 tccgttacgg gaatcggaat gcgacggatg aacaggtgat tcatgcggcg aaacttgcca
     7381 acgccgattt ctttatcact caccttccaa acggttatca gacggtgttg tacgatgacg
     7441 gcgaaaatct gagccaggga cagcggcaat tactggcaat cgcacgcgca gccgtaaagg
     7501 atccgccggt gttgatttta gacgaagcga ccagttccat cgataccaga accgaagctt
     7561 tgatcgaaaa aggtatggat caattaatgg aaggacgaac ggtttttgtg attgcccacc
     7621 gcctttcgac ggtaaggaat tccaatgcca tcttggtttt ggaacacgga gaaattatcg
     7681 aacgcggtga ccatgaggat ctgctggcac aaaaaggtcg ctattatcaa ctttatacgg
     7741 ggatgttcga gctttcctga tcgagccctc aagtggattc aacgcactcc cgatttttcc
     7801 ttttttgaac tgtgatttga ttcaatcaaa tccggacaaa acgagggaaa aagggagtgt
     7861 tatgattaat acagaataac ggttaaacac acgatttaaa agattctatt cggaatcatt
     7921 ctgaatggtt gatagaatca aaatgaaaat cggacgatca tgacagatcg atttctgttc
     7981 tgtatggagg atatgcatga attacggatt cataaaagca gcggcctgta cgccggaaat
     8041 cgttgttgcc gattgcattc ataatgccca agctatcctg gatctgatga aaaaaagcgc
     8101 ggcagaagga gtccggttgg ctgtcttccc ggaattgtgc ctgaccggtt atacctgcgg
     8161 tgaccttttt acccaacaaa cattgttaga atccgtaaaa caaaatctgc tgaaattagc
     8221 cgaggaaagc cgctcccttc cgatgatttc cgttatcgga gcgccgatcg ctcattacgg
     8281 gaagttatat aactgcggtg tggtcctgta tggcggaaaa attttgggaa tcgtccccaa
     8341 aacggaaatt cccaattatg ccgaatttta tgaagcgcga aacttctgtg ccgctcccga
     8401 aacgaacggg acagtccgca tcggagagga tgaaattcca tttggaaaaa gattgctctt
     8461 ctgctgtaac gaaatgcccg aatttatttt cgggattgag atctgcgaag acctttgggt
     8521 ttgccagccg ccgtcaaccg gtcacgccat agccggcgcc accctcatcc tgaacttatc
     8581 tgccagtaac gaaatcgtcg ggaaggctga ctttcgccgg caacttgtgg aaagtcaatc
     8641 cgcccgcctg gtctgcggtt atctctatgc ggatgcgggt gaaggagagt cgacaaccga
     8701 tctggttttt tccggacaca atctgatcgc tcaaaatggt cgcattttgg cagaagctca
     8761 acctttttca agcggatact gtgttaccga actggatttg gaagaactca catcagaaag
     8821 gcaacggtta accacatttc ctcgacaaga cgcgaccggt tatcaaatga ttcccttcaa
     8881 tatggagaaa actcccgtcc agttaacacg caatatttcc aagaatcctt tcgttcccgc
     8941 cgattccgcc gatttacggg atcgctgcga aaaaattctc gctattcaaa gcgccggatt
     9001 gcggaaacgg atcaatcata ctcatgttaa aacagtggtg atcggagtct ccggcggatt
     9061 ggactcaacg cttgccctgt tggtcagtgt tcgagccatg aaggaatcgg gacatcttcc
     9121 ctccgatgta attgcggtta cgatgcccgg ttttgggaca actcgcagaa ccaagaacaa
     9181 cgccattaaa ctatgcgaat cacttggggt aaccttgcga aaaattgaca ttacccgcag
     9241 tgttcgcagt catttccgcg acttgggaca agacgaaaat gatcataatg tcctctttga
     9301 aaatgctcaa gcccgagaac gaactcagat cctgatggat atttgccatc aaacggacgg
     9361 atttgtggtc ggtaccggag atctttcgga attagccctg ggctgggcga catacaacgg
     9421 cgatcacatg agtatgtatg gggtgaacgc ctccgtaccg aaaacattgg tacgatattt
     9481 ggtacgctat tatgcggatt atctggcgga caaacaagtt tccgagattc tttcggatat
     9541 tctgaataca cccgtcagcc cagaattatt acctgccgcg gaagggaaaa tttcgcaatt
     9601 gaccgaaaat atcatcggtc cgtatgaatt acatgatttc tttctctact atttcatccg
     9661 gcacggtttc tcccccgaaa aagtcgcttg gctggcgaat tatgcattca aagatactta
     9721 tagcgctgac gaaattcaaa aatggctgaa agtttttatc aaacggtttt tcaataatca
     9781 gttcaaacga tcctgtcttc ccgacggacc gaaagtcggt tcggtttcgc tttcgccgcg
     9841 aggggattgg cgcatgccaa gcgatgcctc ttttaaaata tggatcgatt cgcttgaccg
     9901 ataaaccgga gatttttcat tcatgaccat cgactcgtgt aatcgaattc gaaaaaaaat
     9961 cgtaaaaatt gtgttgccga tcttattttg cactgtctgt tccggttgtt atttgattga
    10021 gacgatacaa ccgacaccgt tgccgcaaaa aacacccact tccctcatca atatcaatgc
    10081 gcttccgact ttgccgaccc ttccgccggc ggttgagacg gctacgccca ttccggaatc
    10141 cgactttacg tatgacacaa ccggtattat ttttgatcat ttaacggaag aaccggttcc
    10201 tacggaaccg aatcaggcag ccagagtgat cggaaccgta tcggaatttt ccggttcgga
    10261 tctgatcatt aagaattcat cgggagacag ttacacgata acctgcgatg atttttgctt
    10321 ttatgtggat aatcggaaaa aaatcatctc gggagaagcc gttcggccgg gagctgagat
    10381 cgccgttttc ggtgcgaccg attccgaaaa accggaaaaa atccacgcgg atgcgattat
    10441 actgaatggg acggtaaaat ctgataaaac cccgaaggct gatatgagtt atttgccaaa
    10501 cggaatgacc tatcaggaat ttgaacttgc ttcacctcct ttaccggctc ctctccggct
    10561 caatccgctg gaaggatcac tcgacgacca tttaaataag cgtaaaatga caaccctcag
    10621 cgaacggacg aatttttcct atggttttta cggtgaaacc tattccactt cattagaata
    10681 tgatactgaa gggaaccggg atccctatta cccgacacgt tccaacctaa caatctattc
    10741 caacggatat gaattttaca gtttttggtt cccgcatgtc gaaagtccgt ttttttataa
    10801 ttggggaatt gtaaattacg gcggggattg gtatttgccg atccggatga cggttgatat
    10861 caatccggat cccgatatca cggaattaat ctattctgac cgcacgatta tgagtcaaat
    10921 gaattttgat aagtcattca actacatccg ttctttcggc ttttcgatca ttggttacaa
    10981 tttattctat ttttatcaac gtccaggggg atatgggata gcgatcaatc gggtggatta
    11041 tccactcaat tttgatgaga ttattttcgg attggtcggt gattatcagg atttaaaccc
    11101 gttttattcg gatacgctga tcactttctt cggcttgcgc gggaatacat ggtactatgt
    11161 tgagctgacc gcaccggaag agtaatagtg tagtaataca ggattgaagt atggcgaaag
    11221 gatacggatc ggaaaagctt gtttcgtttt cctttcgttc ataagtttta atgattttta
    11281 ttgatttttt tctcggacgg cacaagtata attaatcaga aatttggaaa aattctgcta
    11341 tctcgaataa ctacggagga atcgcatgag tctgaaaatc aaattcggta cggatggctg
    11401 gcgggcatat atcgccgaag attatacatt tgataattgc cggcgctgcg cccaaggttt
    11461 cgcgaattat ttgttgaaaa aggacaaaaa aggagaatgc gttgttgtcg gatatgacaa
    11521 gcgttttcat tcggaaaatt tcgcagccgc ctgcgcggaa gtcatggcgg gaaacggatt
    11581 taatgtcctt ttgactcagg aagcgactcc gacacccgtt atttcctatg cggtcgtcga
    11641 taaaaaagca atcggagcta tcaacattac agcttcccat aaccctcctt cggataacgg
    11701 attcaaagtc cgagatgcga ccggtggtgc gattgacccg caggggcttt tggaaataga
    11761 agccggtata ccggatgata tgagcgatgt caaacggatt ccgctgaatg acggtctgga
    11821 atccggaaga attgaattct tcgacgcttc tgtcaactat atcagtcacc taaaagatct
    11881 gattgatttg gaaccgattc gaaaatccgg tttaaaaatc ctcgtcgatc cgatgtgggg
    11941 aaacggatcg ggctggttca agcgattgct gggtggagat aagacagaaa ttattgaaat
    12001 tcacaatgaa cgaaatccga tctttccgga aatgaaacgc cccgagccta ttccgccgaa
    12061 tattgatgta gctctgaaaa agacggtcga attgggtgcc gatgttcttc tgatcaccga
    12121 cggggatgcg gatcgctgcg gtttgggaga tgaaaacggc gttttcttga accaactgca
    12181 ggtaatggct cttttaaccc tatatttact cgaaattcgc aaagagcggg gagcgattgt
    12241 caagaccctt tccacaacaa cgatgctcaa taaattggct aagatatatg gtgtcccgat
    12301 ttatgaaacc ggagtgggat tcaaatatgt cgcgcctaaa tttaaagaga cagacgcaat
    12361 catcggggga gaagaatcgg gcggatatgc cttccgcggg aatgttccgg aacgggatgg
    12421 gatcctcgct aatctttatt tccttgattt tatggtaaaa accggtaaaa ccccatctca
    12481 attgcttcaa tggttgtttg aaattgtcgg tccccattat tatgaacgaa tcgatacctc
    12541 ctttaaagga aacgccgaag aaaagaaaaa atttattctc gatcatattc cggaaaaatt
    12601 aggcgggatt aaagtgaccg gactggatac cacggatggc tttaaattca tgatggaaga
    12661 cggcggttgg atgttgattc gattttccgg taccgaaccg attattcggg tttatacgga
    12721 aaccactcat gaagattgcg tagacgcgat tttgaaagac ggattaaaag ttgcgggaat
    12781 tgaataatac cgattattgg atcgatactc attgccatct gaatctgccg gactttttgc
    12841 ctgatcaagc agcggtcatc gaccgagccc ggcaggcaaa tgtccgcagg ataattgtcc
    12901 cggggatcga tccggaaagc agtgaaacag caaaaaaaat ggcagaagcg tatcctgccg
    12961 ttttttttgc ggtcggcgtt catcccaatg aatcagccga ttgggatgat tcccggcttc
    13021 aggatctgaa aattttggct acccatccga aatgtgtcgc cgtcggagaa atcggattgg
    13081 atttctaccg tgaattttgt cctcctgcgc aacagttgcg atcgctccat gcccaattgg
    13141 aattggcgga agaaatcggt aagcccgtta tcctccattg ccgtcaagct tttgaagtgc
    13201 tttggccggt attgcaagat tggcatcgac atcattcgaa aaatatcggg gttctgcatg
    13261 cattcgaaga atcaccggaa gccgcagcag aggtgaccgc gcaaggtttc tatgtgggga
    13321 tcggcggagc gtacacttat aagaaaaaga aacatctttc ggaggttctg aaaacggtca
    13381 gtttggataa aattttactt gagaccgact cgccttatct gacacccact ccttatcgcg
    13441 ggcaacggaa tgaaccgatg tacatcccat tgattgcgga aaaagtggcg tcgactcgga
    13501 atatctcaac cgaagcgctt tccgaaatcg tccgggaaaa caccttcaga ttattcggaa
    13561 atttataacc ggatttcaaa agaattttga cttttgacat tcgtttatca tattccgttg
    13621 aagagtatga cggaataagt catatgtgcc atcggtctaa cttttttctg caatttttgc
    13681 atttgcaaaa taattaaaga tttgttaatc ctgataaccc aataaaaaaa tacggtaaaa
    13741 tttgaaagtg agttcatttt aagctgaa
//
LOCUS       DAOSMM010000059        13622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4533_length_13622_cov_14.923270, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000059 DAOSMM010000000
VERSION     DAOSMM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13622
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4533_length_13622_cov_14.923270"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PK898_08215"
     CDS             <1..513
                     /locus_tag="PK898_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU20053.1"
                     /translation="DFWQAGKRSGLRGIPREPWFIKRYISSLFALYLIHLVVRWIDWR
                     EKMLTFNHFNFNVLDLERSLKFYDEALGLKPVREKNAEDGSFKLVYLGDGVTDFQLEL
                     TWLRDRKEPYDLGDLEYHLAMMSDNFEADHERHQKMNIICFENNKMGIYFINDPDGYW
                     IEIVPDRSKK"
     gene            686..1552
                     /locus_tag="PK898_08220"
     CDS             686..1552
                     /locus_tag="PK898_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU20054.1"
                     /translation="MKKSFIVMSLTVILAMLASVTVFAADFDPSKDISVISREEGSGT
                     RGAFIELFGIEQKNDKGEKIDMTTEEAIISNSTNVVMTSVASDPYAIGYISLGSLNET
                     VKALKIDDAEATVKNIRDGSYKIFRPFNVAYKGQLSAVTQDFVNFIMSADGQAVVEAA
                     GYIKVDENAAAFSSTNPSGKIVVAGSSSVTPVMEKLKEAYLKINTAAEIEIQMSDSTT
                     GMTSAMDGICNLGMASRELKESELAVLTPLVIANDGIAVIVNNNNPIEGLTSEQVKGI
                     FTGEITSWSELQ"
     gene            1628..2476
                     /gene="pstC"
                     /locus_tag="PK898_08225"
     CDS             1628..2476
                     /gene="pstC"
                     /locus_tag="PK898_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOU20055.1"
                     /translation="MKKNKEKWMQIIFLLSACTSILAVFLICYFLFANGVPAIGKIGI
                     GNFLLGKVWKPTNNLFGIFPMILGSIYVTAGAIVIGVPIGIMTAMYLALFCPKEIYKI
                     IKPAIELLAGIPSVVYGFFGMVVIVPFVRESLGGNGSSMLSASLLLGIMILPTIINVS
                     ESAIRAVPENYYEAGRALGATHERSAFNIVLPAAKSGVLAGIILGIGRAIGETMAVIM
                     VAGNQARMPKGLLKGVRTLTANIVIEMGYAADLHREALIATAVVLFVFILMINLSFSM
                     LKRKSL"
     gene            2473..3357
                     /gene="pstA"
                     /locus_tag="PK898_08230"
     CDS             2473..3357
                     /gene="pstA"
                     /locus_tag="PK898_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOU20056.1"
                     /translation="MTEMTAMQKINQITFKQQLRTYKRDPFSLLLVFLTYLCAILTIG
                     VLFLLVGYILIKGVPHLSRDLFALNYTSENVSLFPALINTILIVFLSLLVAVPLGIFS
                     AVYLTEYAKKGNKLVGLVRITAETLSGIPSIIYGLFGLLFFVGSLGMGYSILAGSLTL
                     SIMILPLIIRTTEEALSAVPNSYREGSYALGAGKMRTVFQIVLPSAMPGILAGIILAI
                     GRIVGESAALIYTAGTVADIPGDLLSSGRTLAVHMYALSTEGMHVDQAYATAVVLIIV
                     VIIINWLSSVSMKKFVKG"
     gene            3361..4110
                     /gene="pstB"
                     /locus_tag="PK898_08235"
     CDS             3361..4110
                     /gene="pstB"
                     /locus_tag="PK898_08235"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOU20057.1"
                     /translation="MDKFTIENLNLFYGNFQALKQINLKISKNEITAFIGPSGCGKTT
                     LLKTLNRMNDLVEDVTINGKVLLDDEDIYGIMDVNLLRKRVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIRSKAKLDEIVEKSLRDAAIWDEMKDRLKKSALGISGGQQQRLCIARAL
                     AVEPEVLLMDEPTSALDPISTSKIEDLVLELKNQYTIIIVTHNMQQAVRISDKTAFFL
                     LGEVIEYDTTESIFTMPKNKKTEDYITGRFG"
     gene            4110..4778
                     /gene="phoU"
                     /locus_tag="PK898_08240"
     CDS             4110..4778
                     /gene="phoU"
                     /locus_tag="PK898_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOU20058.1"
                     /translation="MRAKFDEQLDLLNSELIMMGGLCEEVIYKAANAMLKGDPVLARE
                     VFPIDSEIDRKEHDIESLCLKLLLQQQPVAKDLRLISAALKMVTDMERIGDQAAEIAE
                     IIQLSNIPFENNNVQIRDMAEATINMVNGSIAAFVQRDLELAQAVIDYDDVVDELFND
                     IKNELIAEIGRRDLPFEGSKFGEFAVDMLMVAKYFERIGDHAVNIAEWVEFAITGQHK
                     DVAD"
     gene            4778..5449
                     /locus_tag="PK898_08245"
     CDS             4778..5449
                     /locus_tag="PK898_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004057096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU20059.1"
                     /translation="MIYCVEDNTEIRELVVYTLQMTGFTARGFVDGKDFFAAIKEELP
                     ELVLLDIMLPGEDGLQILKKLRASEVTRAIPVIMLTAKTTEYDKVIGLDSGADDYLTK
                     PFGMMELIARIKAVLRRASVAEESDEITIGPLVVYRDEHRVFNNGVEVALTPKEFSLL
                     LYLIENRGIVLTRERLLSEVWGFDYYGETRTVDAHIRSLRMKLGEAGDLIETVRGIGY
                     RIEKL"
     gene            5450..7123
                     /locus_tag="PK898_08250"
     CDS             5450..7123
                     /locus_tag="PK898_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU20060.1"
                     /translation="MRQKIFRNMVFLAVLSVLIMAILITFVQYEQFYREMRSAVKNSA
                     VSLSDLVNNQGAGLLNLIQKSPEANRITLVDVDGTVLFDNKSDPAKMENHLDRPEIQA
                     ALKNGQGENERLSETISEITYYYAVKLDDGKILRISRTRKSVWATVFSTIPYFVLIIV
                     IILVLAIFLARKLTKNIIDPLNNLNLDDPLSNDHYDEISSLISRIYNQQQEIQSYVSE
                     VREKQEEFNSVVNNMREGLIILNANANIMTVNRSVGEIFDIEPESYIGKKIYTLHRSS
                     ALQSVIEKAMKGVSAEEVIEVGKRHYQLFADPVKDGAQLKGIILLILDVTEKKEAESN
                     RREFTANVSHELKTPLTSIRGYAEIIRDGIVKPQDIPEFSDRIYKEASRMLEMVNDIM
                     SLSHLDEGLTEEKFEETDLFSIVTEVAEKLKPVAAKKDIQILTEGETSIISGIPRLVF
                     EIVYNLVDNAIKYNREKGTVRISVKTDRQFVMLRVSDTGIGISKEDQARVFERFYRVD
                     RSHNRDSGGTGLGLSIVKHAAMVHHAKVELSSQINVGTTITVSFPVRQS"
     gene            7339..10080
                     /gene="ppdK"
                     /locus_tag="PK898_08255"
     CDS             7339..10080
                     /gene="ppdK"
                     /locus_tag="PK898_08255"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOU20061.1"
                     /translation="MTEKKWVYLFSEVDQAEAYCGGDWDKVRGLLGGKGANLADMARI
                     GVPVPPGFTVTTEACNYYSAHGKFPEGLWEQMLAALAEVEKAAGKKFGDQKNPLLVSC
                     RSGAKFSMPGMMDTVLNIGLNDETALGMVELTGNERFVYDSYRRLLQMFGSVVLEIRD
                     EAFEKPLEEYRAKKGYKLDTEMTAEDWKALCETYKKIVRVETGHDFPQDPYEQMRLAT
                     VAVFNSWNGKRAVDYRRAEKIPDDLGTAVNIVTMVFGNMGEDSGTGVAFTRNPLDGTK
                     KIYGEYLLNAQGEDVVAGIRNTQPLETLQTVMPDVYTQFVDIASRLEKHYRDMQDMEF
                     TIERGKLWMLQTRNGKRTARAEVAIAVQMVNEGLIDKETALKRVTTKQVDALLHPQFD
                     PEALKKIEPFATGVNASPGAAVGQIYFDADTVVEMKETENKDCIMVRQFTKPDDVHGM
                     LSAKGILTSEGGATSHAAVVARQFGVPCVVGASSLKIDLERKTVTAGDLVLHEGDWIS
                     VDGTTGKVYAGKVNVVVPDINKMTDLNTLLSWADEVADLEVWANADNPRDASRARTFG
                     AKGIGLCRTEHMFFETERLPIVQRMILSKTSEERTEALNELLPYQRADFDGLFEAMSG
                     YPVIIRLIDPPLHEFLPSPEALREQIIEKRIQKFSDYVSGKAADKELVNLENLLIEVE
                     KRHESNPMMGLRGIRLSIVMPEIVEMQVRAIFEAACDVKKRGFEPKPEIMIPLTGHVN
                     ELKTVQPRLIELAKQVMAEKGVEVEYLFGTMIEIPRAAVTAGEIAQIAQFFSFGTNDL
                     TQMTFGYSRDDAEHSFLITYVEKGILPANPFQEIDRDGVGKLMRMAIRDGREVRPDLK
                     VGICGEHGGDPSSIEWCHIIGNDYVSCSPYRVPIARLAAAQAKIKFPDPEDRRNIKK"
     gene            complement(10177..11091)
                     /locus_tag="PK898_08260"
     CDS             complement(10177..11091)
                     /locus_tag="PK898_08260"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOU20062.1"
                     /translation="MYKTDLSVTIKSLKMKNPVMPASGAYDYFENNANVFPMDILGAI
                     MLKSIHRRARPGNKPPRIAEVTGGMLNAVGIPSAGIEAYLRDNSLARYRELNVPVIVS
                     VSGDQPRDYVETCRMLAGNPSVDAIELNLSCPNVGTGLQLATDEVLLGRTVEATRLVT
                     DFPLIVKLSPNVTDICKPAVIAQDCGADAVTIANTFMGMKIDIKTRKPFLGNTTGGLS
                     GPAIKPITLRMVYQAYQKISIPIIGCGGIMCAADAIEYILAGATAVQVGSGNFVNPML
                     MKEVIEGMDAYLAENHFEKLEDIRGAAH"
     gene            complement(11093..11857)
                     /locus_tag="PK898_08265"
     CDS             complement(11093..11857)
                     /locus_tag="PK898_08265"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOU20063.1"
                     /translation="MIQNCTVVKNTTICPGYGRIVISAPEISATALPGQFVMTKYWDG
                     NSPFLLRPFSINAVDRNAGTIEILYKVIGEGTRLLLRLKEGADFLILGPLGNSFPLNP
                     NFSRIGIVGRGVGAAPMRFLAEEAIKQGKDVFVYISASKEEYLFDRRYYQEMGAHFIG
                     SCDPDKMVTSFLEEDLKSNHFDAAYTCGSNRLIRDIKRLMDTYRLEGYASLEAHMACG
                     VGACKGCVCTIFENGEEKYAHVCKDGPVFPIQKLVK"
     gene            complement(11861..12601)
                     /locus_tag="PK898_08270"
     CDS             complement(11861..12601)
                     /locus_tag="PK898_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20064.1"
                     /translation="MMLSLENIYVNYGSIQVLHGINIEVNEGEIVTIIGANGAGKSTT
                     LKAIAGLVTKTRNSCIKFMGEDITNLPPEKIVAKGISLAPEGRRIFPDLTVLENLEMG
                     AYLRAKDKQRVKKDMESIFELFPILEQRRNQSGKTLSGGEQQMLAIARALMANPKLLM
                     LDEPSTGLAPLIIKDIFGIIKQVNEKGTTVLLVEQNSKIALGIADRGYVLKNGVVVLQ
                     DTCENLMKNEEIKKAYLGEGAYANAVKG"
     gene            complement(12598..13365)
                     /locus_tag="PK898_08275"
     CDS             complement(12598..13365)
                     /locus_tag="PK898_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20065.1"
                     /translation="MDILETKGIGIHFGGLRAVDNVSFTAEEGSITAIIGPNGAGKTT
                     LFNLIAGFYKPTEGSVFYEGRDITGVKTFRRTYMGIARTFQNINLFKDLSVMDNVLVG
                     LHSKTKCDPISSMLNLPNKQKEEKESREKVMETLKFLNLDHYVNEKAGSLPYGLQKNL
                     EIARALVSKPKVLLLDEPASGLNTNDLESLSRRIQAIRESGITVVLIEHKMDVVMSIS
                     DKIIVLNFGVKIADGKPEEIAKDPKVIEAYLGKDDEK"
     gene            complement(13365..>13622)
                     /locus_tag="PK898_08280"
     CDS             complement(13365..>13622)
                     /locus_tag="PK898_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20066.1"
                     /translation="MMVVGGIGSIPGAILGGFGLTLLPEFMRFLGNIRLVLYGAAIVA
                     IIILAPKGLGGLIEWIDGVLCGEIKPGVSKEKAVSANEEMK"
BASE COUNT         3640 a         2998 c         3368 g         3616 t
ORIGIN      
        1 gatttttggc aagccggaaa gaggagtggg ctgagaggga ttccccgaga gccatggttc
       61 atcaaacggt atatttcctc cctatttgcc ttatacttaa ttcatcttgt tgtcagatgg
      121 atcgattgga gagaaaaaat gcttacgttt aatcatttca attttaatgt tttggatctt
      181 gaaagaagtc tgaaatttta tgatgaagcc ttaggcttga aaccggtacg ggaaaagaat
      241 gccgaggacg gatctttcaa gttggtctac ctcggtgacg gcgtaacgga ttttcagttg
      301 gaactcacct ggttacgcga tcggaaagag ccttatgacc tcggtgattt ggaatatcat
      361 ttggcaatga tgtccgataa ctttgaagcc gatcatgaac gccatcaaaa gatgaatatc
      421 atctgcttcg aaaataataa aatggggatt tactttatca atgatcccga cggctattgg
      481 attgaaatcg tgccggatcg cagtaagaaa tgaacgaaat caacggttga agcagaaaag
      541 ccttcaaccg gttataccca tttcgcaaaa aaagccgaac cgtttttatg gttcggtttt
      601 ttttacagaa actttacaaa gaaactcata ttccctttac atccgttcga tatgatggag
      661 ttgataaaaa aataaaggag aaaatatgaa aaaaagtttt atcgttatga gtttgacagt
      721 cattcttgcc atgttggcat ctgtcacggt ttttgcggct gatttcgatc cgtccaaaga
      781 catttccgta atttcacgcg aagagggttc gggaacccgc ggcgccttca tcgaattgtt
      841 cggaatcgaa caaaaaaatg ataagggcga aaaaatcgat atgactaccg aggaagcgat
      901 catttccaac agcaccaatg ttgtaatgac atcggtcgca agtgatccct atgccatcgg
      961 ttatatctca cttgggtcct taaatgaaac cgtaaaagca ttgaagattg acgacgcgga
     1021 agctaccgtg aagaatattc gagacggcag ttacaaaatt ttccgcccgt tcaatgtcgc
     1081 ttataaaggt caattaagtg ctgtcacaca ggattttgtc aattttatta tgagtgcaga
     1141 cggacaggcg gttgttgaag cggcgggata tatcaaagtc gatgaaaatg cggctgcgtt
     1201 ctcttccaca aatccttccg gaaaaattgt tgtagccgga tcttcttcgg taactccggt
     1261 aatggaaaaa ctgaaggaag cttacctgaa aataaataca gccgcggaaa tcgaaatcca
     1321 aatgagtgat tccacaaccg gcatgacttc cgcgatggac gggatttgca atctgggaat
     1381 ggcttcccgc gaattgaaag aaagtgaact tgcggttttg accccgttgg ttattgcgaa
     1441 tgacggaatc gctgtgattg tcaataacaa taatccgatc gaaggtttga cttcggaaca
     1501 ggtgaaagga atttttaccg gtgagattac gagttggtcc gaattacagt aatcgaacgg
     1561 tcaggtttat tccgaatatg gcaggctgtg ttccaacaca gcctgcgttg acgtccgaaa
     1621 ggatcctatg aagaagaata aagagaaatg gatgcaaatc atattcctgc tttcggcttg
     1681 cacttccatt ttggctgttt ttttgatttg ttattttctt tttgcgaatg gggttcccgc
     1741 gattggaaag atcgggatcg gcaatttctt gttagggaag gtctggaaac cgaccaacaa
     1801 cctgttcggt atattcccga tgatattggg aagtatctat gtcacagccg gagcgatcgt
     1861 tatcggtgtt cccatcggca ttatgacagc tatgtatctg gcacttttct gcccgaaaga
     1921 aatttacaaa atcattaaac cggccatcga attactggcg gggattccgt cggttgttta
     1981 cggcttcttc gggatggttg tgatcgtccc gtttgttcgg gagagtttgg gtgggaacgg
     2041 aagcagcatg ctttccgcct ccttattatt gggtatcatg atcctgccga ccatcatcaa
     2101 cgtcagcgaa tcagccatcc gggccgtgcc ggaaaactac tatgaagccg gtcgagcatt
     2161 gggagccact catgaaagaa gcgcgtttaa cattgtttta ccggcagcca agtccggtgt
     2221 tctggcaggt atcattctcg gaatcggacg ggctatcgga gaaactatgg ctgttattat
     2281 ggtggcgggg aaccaagcac gcatgccgaa aggattgctg aaaggtgttc gaacactgac
     2341 cgcaaatatc gtcattgaga tggggtatgc ggctgattta caccgggagg cattgattgc
     2401 cacggcggtt gtcttattcg tctttatctt gatgatcaat ctttcattct cgatgctgaa
     2461 aaggaaatcg ctatgaccga aatgacagcc atgcaaaaga tcaaccagat caccttcaaa
     2521 cagcaacttc ggacttacaa acgagatccg ttttccttat tgttggtttt ccttacatat
     2581 ctttgtgcaa ttttgacgat cggagtttta ttcttgttgg tagggtacat ccttatcaaa
     2641 ggggttccgc atctttcccg ggaccttttc gctctgaact ataccagtga aaatgtctcc
     2701 ctttttccgg ctttaatcaa tacgattctg atcgtttttc tctctcttct ggtggcagtt
     2761 ccgttgggga ttttttcggc cgtttacctg acggaatatg caaaaaaagg aaataagctg
     2821 gtcggtcttg ttcggataac ggcggaaaca ctatccggca ttccttccat catttatgga
     2881 ttgttcggat tgcttttttt tgtcgggagc ctcggtatgg gctattctat tctggcgggc
     2941 tcattgacgt tgtctattat gattctcccg ctgatcattc gaacaacgga agaagctctt
     3001 tcagcggtac cgaattcata tcgggaagga agctatgcat tgggagccgg taagatgcgg
     3061 acggtgtttc aaatcgtgct gccgtcggca atgccgggaa ttttagccgg gatcatttta
     3121 gcgatcggcc gcattgtcgg tgaatccgcg gcgttgatct atacggcggg tacggttgcg
     3181 gacattcccg gtgatctttt atcttccggc agaacgttgg ctgttcatat gtatgctctt
     3241 tcaaccgaag ggatgcatgt ggatcaagcg tatgcgacgg ctgttgtctt aattattgtg
     3301 gtcatcatca tcaattggct ttcctccgtc agcatgaaaa aatttgtaaa gggataacgg
     3361 atggataaat ttacaatcga aaatctcaac ttattttacg gaaatttcca ggcgcttaaa
     3421 cagatcaatt tgaaaatttc caaaaacgaa atcactgctt ttatcggccc ttccggctgc
     3481 gggaagacaa cgctgttgaa aacattgaac cgcatgaatg acttggtgga agatgtgaca
     3541 attaacggga aagttttact ggatgacgaa gatatctatg ggatcatgga tgttaacctg
     3601 ttacggaaaa gggtggggat ggtattccag aaaccgaatc cgttcccgat gagcatttac
     3661 gataatgtgg ctttcggacc gcggacacac ggcatccgct cgaaagcgaa attggatgaa
     3721 attgtcgaga aatcgttgcg ggatgcagcg atttgggatg aaatgaagga ccgcttgaaa
     3781 aaaagcgcct tgggcatttc cggcgggcag cagcaacgac tttgcattgc cagagcgctg
     3841 gccgtcgaac cggaagttct gttgatggat gaaccgacat ccgcattgga tccgatttca
     3901 acttcaaaaa ttgaagatct tgtgctggag ctgaagaacc aatatactat tattatcgtc
     3961 acacataata tgcagcaggc tgtccggata tccgataaaa ccgctttttt ccttctgggt
     4021 gaagttatcg agtatgacac gacggaatcg atctttacca tgccaaagaa taaaaagacc
     4081 gaagattata ttacagggag atttggctaa tgagagcaaa attcgatgag cagttggatt
     4141 tgttgaattc cgaattgatc atgatgggcg gcctgtgcga agaagtcatt tataaagcag
     4201 ccaacgccat gttgaaaggc gatccggttt tggcacggga agttttcccg attgacagcg
     4261 aaatcgatcg gaaggaacat gacattgaaa gcctttgtct gaaactctta cttcaacaac
     4321 agccggtggc aaaagatttg cgcctgattt ccgccgcgtt gaaaatggtt accgatatgg
     4381 aacggatcgg tgatcaggca gcggaaattg ccgaaattat tcagctctcc aatatcccgt
     4441 ttgaaaacaa caacgttcag attcgggata tggccgaagc gacgattaac atggtaaatg
     4501 gaagcatcgc ggcatttgtg caaagagact tggagctggc tcaagcggta atcgattatg
     4561 atgatgttgt cgatgagtta tttaatgaca taaagaacga attaattgcg gagattggca
     4621 ggcgcgatct gccttttgaa gggtcaaaat ttggagagtt cgccgttgat atgttaatgg
     4681 ttgcaaaata ttttgaacga atcggcgatc atgcggtcaa tatcgccgag tgggtagaat
     4741 ttgccattac cgggcagcat aaggacgttg ccgactgatg atttactgtg tcgaagataa
     4801 taccgaaatt cgcgaattgg tcgtctacac actgcagatg accggtttta cagctcgcgg
     4861 ctttgtcgac ggaaaagatt tttttgcggc gataaaagaa gagctgccag aacttgtttt
     4921 actggatatt atgcttcccg gggaggatgg tctgcagatc ttgaaaaaat tgcgcgcttc
     4981 cgaggtaacc cgggccattc ctgtgatcat gctgaccgct aaaacaaccg aatatgacaa
     5041 ggtcatcggg cttgacagcg gagcggacga ttatctgacc aaaccgtttg ggatgatgga
     5101 attaatcgcc agaattaaag ccgtattaag gcgggcaagt gtggcggaag aatcggatga
     5161 aatcacaatc ggacctctgg tggtctatcg ggatgagcat cgtgttttca ataacggtgt
     5221 cgaggttgcg ctgactccca aagagttctc cttgttgctt tatctgatcg aaaacagggg
     5281 aatcgtatta acgagagaaa gactgttatc cgaggtttgg gggtttgatt attacgggga
     5341 aaccagaacg gttgacgcgc atattcgatc cctacggatg aagttaggtg aggcgggtga
     5401 tttaatcgag accgtacggg gaatcggcta cagaatcgag aaactctgaa tgagacaaaa
     5461 aatctttcga aatatggtat ttctggcggt tttgtccgtt ttgatcatgg caatcttgat
     5521 tacctttgtc caatatgaac agttctatcg ggaaatgcgt tcggcggtga aaaacagtgc
     5581 cgtttcgctt tccgatctgg taaacaatca aggagcgggg ctgttaaacc tgattcaaaa
     5641 aagtccggaa gccaaccgaa tcaccttggt tgacgtcgat gggactgtcc tgtttgacaa
     5701 caaaagtgat cctgcaaaaa tggaaaatca tctggatcgt cccgaaattc aggcagcgtt
     5761 aaaaaatggg cagggcgaaa atgaacggct atccgaaaca atatccgaaa ttacatatta
     5821 ttatgcggtt aaattagatg acggaaaaat attgcgtata tccagaacac gaaaaagtgt
     5881 ctgggcaacg gtattttcga ctatcccgta ttttgtattg atcattgtga tcatcctggt
     5941 tctggcgatc tttttggcga ggaaattaac caaaaatatt attgatccgt tgaataattt
     6001 gaatttggat gatccgctgt ccaatgatca ttatgatgaa ataagctcat tgatatccag
     6061 aatttataat cagcagcaag agattcaatc ttatgtcagt gaagtgcggg agaaacaaga
     6121 agagttcaat tcggtcgtca acaatatgcg tgaaggactt attatcctga acgccaacgc
     6181 caacattatg actgttaacc ggagcgtcgg tgagatattt gatatcgagc cggaaagtta
     6241 tatcggtaag aagatttaca ccttacaccg gagttccgcg ctgcaatcgg ttatcgaaaa
     6301 agccatgaaa ggcgtttccg ctgaagaggt tattgaagtc gggaaaaggc attatcaatt
     6361 atttgccgat ccggtgaagg acggagcgca attaaaagga attattctgc tgattctgga
     6421 cgttacggag aaaaaagaag ccgaatcgaa tcggcgggaa tttacggcaa acgtttctca
     6481 tgaacttaaa actcctttaa cttccatccg cggatatgcc gaaatcattc gtgacggcat
     6541 tgtaaagccg caggatatcc ccgaattttc cgatcggatc tataaagaag ccagccggat
     6601 gcttgagatg gtgaatgaca ttatgtctct ttctcatttg gatgaaggac tcacggaaga
     6661 aaaattcgaa gaaacggatc tgttttcgat cgttacggag gttgcggaga aactaaagcc
     6721 ggtggcggcg aagaaagaca ttcagatcct tacggaaggc gagacgagca tcatttcggg
     6781 aattccgcga ttggtctttg agatcgtcta caacttggtt gacaacgcga tcaaatataa
     6841 tcgggaaaaa ggaaccgtcc gcatttcggt aaagacggac cgccaatttg tcatgttacg
     6901 ggtaagcgac accggcatcg gcatttccaa agaagatcaa gctcgagtct ttgaacgctt
     6961 ctatcgggta gatcggagtc acaatcgaga ctccggtgga acagggttgg gactctctat
     7021 cgtaaagcat gccgcgatgg tgcatcatgc aaaagtagaa ttatccagtc aaataaatgt
     7081 cggaacgaca attaccgttt cttttcccgt tcgacaatcc tgagaaacct tcccgaatca
     7141 gacgctatga catataaaaa tgccttaaga tatttttaaa cttatcggtt acgtcatctc
     7201 tatcataaaa atttatatta ttttcatata agtaagcatt ttgacgggta ctgatgccca
     7261 tatggtaaaa tttatttaca atataccagg atttgataaa aattaatgcg gaaatatttt
     7321 taggaggaag aaagtccaat gactgagaaa aaatgggtct atttattcag cgaggttgat
     7381 caagctgaag cttattgcgg gggggattgg gacaaggtcc gcggtctgct gggtggaaaa
     7441 ggggccaatc ttgcagacat ggcgcgaatc ggtgtacccg ttccgccggg gtttacagta
     7501 acaactgaag cctgtaatta ttattctgcc cacggaaaat tccccgaagg tctttgggag
     7561 caaatgttgg ctgcgttagc cgaagttgaa aaagcagccg ggaagaaatt cggagatcag
     7621 aaaaatcccc ttttggtatc ctgtcgttca ggcgccaaat tttctatgcc cgggatgatg
     7681 gacacggttt tgaacatcgg tttgaatgat gaaaccgcgc ttggaatggt tgaattaacc
     7741 ggtaacgaac ggtttgtata tgactcttac agacgtctcc tccaaatgtt tggttctgtg
     7801 gttcttgaaa tccgcgatga agctttcgaa aagccattgg aagaatatcg agccaaaaaa
     7861 gggtacaaac tggataccga aatgacggca gaggattgga aagccctttg cgaaacctat
     7921 aagaagattg tcagggtcga aaccggtcat gatttcccgc aggatcccta cgaacaaatg
     7981 cgccttgcca cggttgccgt tttcaattca tggaacggga aacgggcggt ggattatcgt
     8041 cgcgcggaaa agattccgga tgatttggga acagccgtca atatcgtcac catggtattc
     8101 ggcaatatgg gtgaagattc cgggaccggt gtagctttca cgcgtaaccc gttggacggt
     8161 accaagaaga tttacggcga atatctgtta aatgctcaag gtgaagatgt cgtagccgga
     8221 atccgaaata cacagccgtt ggaaacactt caaacggtta tgccggatgt ttacacccaa
     8281 tttgtggata tcgccagccg gttggaaaag cactatcggg atatgcagga tatggaattt
     8341 accatcgaac gcggtaaact ctggatgctg cagactcgga acggaaaacg caccgcacgg
     8401 gcggaagttg ccattgcggt acagatggta aacgaagggt tgatcgataa agaaacggct
     8461 ttgaagcggg ttacgacgaa acaggtcgat gctctgctgc atccccagtt cgaccccgaa
     8521 gccctcaaga agatcgaacc gtttgccacg ggcgtcaatg cctctcccgg cgcggcggtc
     8581 ggacagattt attttgatgc cgataccgtc gtcgaaatga aagaaaccga aaacaaagat
     8641 tgcattatgg ttcggcaatt tactaagccg gatgacgttc acggtatgct ctccgcaaaa
     8701 ggtattttaa cctccgaagg cggagccact tcccatgcag cggttgttgc ccgacagttc
     8761 ggtgttccct gtgtggtcgg cgcttcttcg ctgaaaatcg atttggaaag aaaaacggta
     8821 actgccgggg atctggtgct gcatgaaggt gattggattt cggtagacgg taccaccggt
     8881 aaagtttacg cggggaaggt gaatgtggtt gttccggaca tcaataaaat gaccgatctg
     8941 aacacccttc tgagttgggc tgacgaagtc gccgatctgg aagtttgggc gaatgcggat
     9001 aatccgcggg acgcgagccg cgcccggaca ttcggagcca aaggaatcgg cctgtgccgg
     9061 acggagcata tgttctttga aaccgaaagg ttgccgatcg tccagcggat gattctgtcg
     9121 aagacatccg aagaacggac ggaagcgctg aatgaattgt tgccatatca acgggcggat
     9181 tttgacggtt tgttcgaagc catgtccggt tatccggtca ttattcgcct gatcgatccg
     9241 ccgttacatg aattcctgcc ttcgccggaa gcattgcggg aacagattat tgagaagcgt
     9301 atccaaaaat tctcggacta tgtttccggg aaagccgccg acaaagaact tgtcaatctc
     9361 gaaaatctat tgatcgaagt cgaaaaacgg catgaatcga acccgatgat gggtttacgc
     9421 ggaattcggt tgagtatcgt gatgccggaa atcgtcgaaa tgcaggtgcg cgccattttc
     9481 gaagcagctt gtgatgtcaa aaagcgtgga tttgagccga aacccgaaat catgatcccg
     9541 ctgaccggcc atgtcaacga attaaagacg gtacagccgc gtttgatcga acttgccaag
     9601 caggttatgg ctgagaaggg cgtggaagtc gaatatctgt tcgggacaat gattgaaatt
     9661 ccgcgcgccg cggtaaccgc cggtgaaatc gctcagattg ctcagttctt ctcattcggg
     9721 acgaatgact tgacgcagat gaccttcggt tacagccggg atgatgcgga acattccttc
     9781 ctgattactt atgtggaaaa aggaatttta cccgccaacc cattccagga aatcgaccgt
     9841 gacggtgtag gaaaactgat gcggatggcg attcgggatg gacgcgaagt ccggccggat
     9901 ctgaaagtcg gtatctgcgg agaacacggc ggtgacccga gctccatcga atggtgccat
     9961 attatcggga atgattatgt cagctgctca ccttatcggg ttccgattgc ccgtttggct
    10021 gctgctcagg cgaagatcaa attccccgat ccggaagatc gccggaatat caagaaataa
    10081 actttttgat tgacaaaaag ccacctgtaa aaaggtggct tttattttag tcgaaatatg
    10141 aaaaggcttc ggatcagaag cgtgaaatct tgccgattaa tgggcggctc cgcggatgtc
    10201 ctccagcttc tcgaaatggt tttccgccag atatgcatcc attccttcga tcacttcttt
    10261 catcagcatc ggattgacaa aatttccgga gcccacctga acggctgtcg cgcccgccag
    10321 gatatattcg atcgcatccg ccgcgcacat aataccgccg cacccgataa tcggaatgct
    10381 gattttttga taagcctgat aaaccatccg caacgtgatc ggcttaatgg ccggtccgga
    10441 taaaccgccg gtggtatttc ccaagaacgg ttttctcgtt ttgatgtcga ttttcattcc
    10501 catgaaggta ttggcgatcg tcaccgcatc cgcgccgcaa tcctgcgcga tgacagccgg
    10561 tttacagata tccgtcacat tgggggacag cttcacaatc aacggaaaat cggtgaccag
    10621 acgggttgcc tcgactgtcc gccccaataa gacctcatca gtggccagct gcagtcccgt
    10681 tcccacattg gggcaggata aattcagctc aatcgcatcg acggacgggt ttcccgccag
    10741 cattcgacat gtctcgacgt aatcccgcgg ctgatctccc gacacactga cgataaccgg
    10801 cacgttcaat tcccgatacc gggcaaggga gttatcccga agataggctt cgattcctgc
    10861 agatggaatg ccgaccgcat tcagcattcc gccggtcact tcggcgattc gcggcggttt
    10921 gttccccggg cgggcacggc gatgaatact tttcagcata atcgctccca ggatatccat
    10981 gggaaaaaca ttcgcgttat tctcaaagta atcatatgcg ccggaagcgg gcatcaccgg
    11041 atttttcatt tttaaacttt ttatggttac gctcaaatcg gttttgtaca tattatttca
    11101 ccaatttctg aatcggaaaa acaggaccgt ctttgcagac atgggcatat ttttcttcgc
    11161 cgttctcgaa gattgtacac acgcatcctt tacacgcacc cacgccgcaa gccatatgcg
    11221 cttccaacga ggcataccct tccagccgat aagtatccat taaccgtttg atatcccgaa
    11281 tcaggcgatt tgagccgcag gtataggcgg catcgaagtg gttgcttttc agatcttctt
    11341 cgaggaacga agttaccatt ttatcggggt cgcaggagcc gataaaatga gcgcccattt
    11401 cttggtaata tcgacggtcg aacaggtatt cttccttgct cgccgaaata tatacaaaga
    11461 catcctttcc ttgttttatg gcttcttccg ccagaaaacg catcggagcg gcgccgacgc
    11521 cgcggccgac aattccgatc cgtgaaaaat tcggattcaa cgggaatgaa ttacccaacg
    11581 gaccgagaat cagaaaatcg gcaccctcct tcagccgcag cagcaaacga gtcccctcgc
    11641 cgatcacttt ataaaggatt tcaatcgttc cggcattgcg atcaaccgca ttgatactga
    11701 acgggcgcag cagaaacggc gaatttccgt cccaatattt ggtcatgaca aactgaccgg
    11761 ggagagcggt tgccgaaatt tccggagcag aaatgacaat tcttccgtat cccgggcaaa
    11821 tcgttgtgtt ttttactacg gtgcaatttt gaatcattga ttaccctttc acagcgttcg
    11881 cgtaggcacc ttctccgaga taagccttct taatttcctc gtttttcatc aggttttcgc
    11941 aggtatcctg aagaacgacc actccgtttt tcagcacata accgcgatcc gcaattccca
    12001 gcgcaatttt tgaattttgt tcgacgagga ggactgtggt tcctttttca ttcacctgtt
    12061 tgataattcc aaagatatct ttgataatca agggcgccaa tccggtggaa ggttcatcta
    12121 acattaacaa tttggggttt gccatcaaag cgcgtgcgat ggcgagcatc tgctgttctc
    12181 cgccggaaag tgttttcccg gattgattgc ggcgttgttc caaaatgggg aacaattcaa
    12241 agatagattc catatccttc ttgacgcgct gcttgtcttt ggcgcgcagg tatgctccca
    12301 tctccagatt ctccagaacg gtgaggtccg ggaaaatacg ccttccttcg ggggctaacg
    12361 aaattccttt ggcgacgatc ttttcgggag gaaggtttgt gatatcctca cccatgaatt
    12421 taatgcaact gttccgggtt ttggtcacca atccggcaat tgctttcaaa gtcgtacttt
    12481 ttccggcgcc gtttgcaccg ataatggtta cgatttcacc ttcattgact tcaatattga
    12541 tcccgtgcaa tacctggatg ctgccgtagt tgacatagat attttccaga gatagcatca
    12601 tttttcatcg tcctttccaa ggtatgcctc aatcactttc ggatcttttg cgatctcttc
    12661 cggttttcca tccgcaattt tcaccccgaa attcaatacg atgattttat cggaaatgga
    12721 catcaccacg tccattttat gctcgatcaa cacaaccgtg atgccgcttt cccggatcgc
    12781 ctgaattctg cgcgaaagtg attccaggtc attggtattc aacccggatg cgggctcatc
    12841 cagaagtaac actttgggtt tggaaaccaa agcgcgggcg atttccagat ttttttgcag
    12901 accgtaggga agactgccgg ctttttcgtt cacataatga tccaggttaa gaaacttcag
    12961 cgtttccatt accttttccc tgctctcttt ttcctctttc tgtttattcg gcaggttcag
    13021 catcgagctg atcggatcgc atttggtttt ggaatgaaga ccgacgagga cgttatccat
    13081 taccgaaaga tccttgaaaa ggttaatgtt ctgaaatgtt cgagcgattc ccatataggt
    13141 tctgcgaaat gtcttcacac cggtgatatc tcttccttca taaaagacag aaccttccgt
    13201 cggcttataa aaaccggcaa tcaggttgaa caaggtcgtt ttgccggcgc cgttcgggcc
    13261 gatgattgcg gtaatactgc cttcctccgc agtaaaggag acgttatcaa ccgcccgtaa
    13321 gccgccgaaa tggattccga ttccttttgt ttcgagaatg tccattattt catctcctcg
    13381 ttcgcggaga ccgctttctc tttgctgacc cccggcttga tttctccgca gagcaccccg
    13441 tcaatccatt cgatcaatcc gcccagccct ttcggtgcca gaataattat cgcgacgatc
    13501 gcggccccat agagaaccaa gcggatattc cccaaaaagc gcataaattc cggaagcagc
    13561 gtcagcccga aaccgccgag gatcgcaccc gggatactgc cgattccgcc gactaccatc
    13621 at
//
LOCUS       DAOSMM010000060        13498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4586_length_13498_cov_13.397902, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000060 DAOSMM010000000
VERSION     DAOSMM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_13498_cov_13.397902"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PK898_08285"
     CDS             <1..999
                     /locus_tag="PK898_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20067.1"
                     /translation="FREMTVPYYPVTIFYAFKQAGNQNEERQTEEFSLDNERVSTGWE
                     TMLNGLLASSFEITGTWPMRTELANRSVGIGANALASSIVLVCRPRAEDAPSVSRREF
                     LAALNRELPAALHRLQRGNIAPVDLAQAAIGPGMAIFSRYSAVLESDGSPMSVHTALA
                     LINRTLDEYLAEQESEYDSDSRWALAWFEQFGHEAGPYGIAETLSKAKNTGIERLTHA
                     GILEARGGTVRLLRRDELAKDWDPSVTKRVTVWEATQYLIRALDEDGEIGAGSLLGKL
                     AEYGETIRDLAYRLYTLCERKGWAQDATAYNMLIIAWPRLKNLSDNRTESVQESLL"
     gene            1039..4401
                     /locus_tag="PK898_08290"
     CDS             1039..4401
                     /locus_tag="PK898_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HOU20068.1"
                     /translation="MAVSNIENIGKGLNILKEGLAPYIRRELKEYYKGNWWTDGIERA
                     LQYNAGSNALSGNGSPDERFDLLDIQALLMIMWENWNEVFKTELGHTGRSYVSELREI
                     RNKWAHQQPFDADETFRALDTMLLLLEMVNGSGQEELRGMQYEFMRKKFDAETKRELK
                     KSTENLSNGVSAGLKPWREIVTPHHDVASGHYQQAEFAADLFQVISGKAEPEYLDPVE
                     FFRRTYLTEGLSRLLSQAWIRLTSAGGDPVVELQTNFGGGKTHSMLALFHLFGGQIKP
                     AQVPELEKLVPAGLKGGELDLPVASRAVLVGTQLSPSEVRTKADGTQVHTLWGEMAWQ
                     IGDANGKAHEAYELVAKEDQDGISPGSEKLAELFAQFGPVLVLIDEWVAYTRQLYGKE
                     NLPGGSFDSNITFVQALTEAAKAVPDTMVVAAIPESDIEIGGEGGKAALERIQNVFGR
                     VESVWKPASSTESFEIVRRRLFQPISEADYASRDAVCRKFAEMYQVNRAEFPTECREM
                     DYEKRMIATYPIHPELFDRLYEDWSTLERFQRTRGVLRMMAAVIYRLWDNRDNSLLIM
                     PCTVPLDWMDVRSEITRYLPEGWGAVIDKDVDGPQSQPVKLDRENPNLGRYSACRRVA
                     RTIFFGSAPSASSKKIHGIEEVRIKLGCVQPGEQPSVFGDALRRLSEQLTYLSSESTR
                     YWFDIRPTIKKIASDRAAQYEHKPEIVEDEITKRMKSSVRNDRGDFAGVHAFPKNSGD
                     VPDEQACRLVILNPSSVQRKQSKESDAIQAAQDILENRGNIPRLYRNMLIFLAANGDR
                     LPDLENAVRNWLAWTSIQQDEEQLNLDSHQKREVENQIREYDNRINAQLSETFCFLLV
                     PTQEGTDPVNWTIYRLQNGDQLVSRASKKLKMEEQLITDWAPALLRRGLDRWLWKDEN
                     HIHIDKLWGKLAQYLYLPRLRDEEVLIKTIRNGVNSTDWEEYFAYASGLKSDGTYLGL
                     IAAEIPSVAIDHESLLVKPEVAKKQLEKTGKTRLGQGEYVNLAELSPNSVNDRLDFIG
                     RSSNEGESEQGKKTFIHRFHATVELDPKRLGRDASNISEEVLTHLTSNVDTKATVTLE
                     IDIDVPGGISEDKIQIIKENCNTLKFKTVDFE"
     gene            4803..6122
                     /locus_tag="PK898_08295"
     CDS             4803..6122
                     /locus_tag="PK898_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU20069.1"
                     /translation="MLIEFTVGNYRSFKDPVTLSMVAAKLNSKYPEIDESNTFQAEQG
                     LTLLSSAAIYGANASGKSNLIRAFQLMRDFIQNSSKESLADEPIHTTPFRLDSKAEHS
                     PSYFEVVFRLDDIRYRYGFEITTEKVETEWLFYSPKGKESKLFTREGNDISFSRAFKG
                     NKTALISLTRPNALFLSVAAQFNNEIATKVLGWFLSVNVISGLDDTGYRGFTTMAFAK
                     NKTYREKIIQFIRGFDVGIDSIIIEKAKISESRMFPKDLPQELKDFFLKQIKEGDELI
                     NFRPIHRKLCENGALEETKFDLTDESEGTQKLFYLSGPIIDTLLGGKVLFIDEIEARL
                     HPLLTRKLIGLFHSKELNPRHAQLIFATHDTNLLSNKLFRRDQIWFVEKDEEGASHLY
                     SLAELKVRNDASFGSEYLQGRYGAIPIFGEFKETLMDFEREDHQDEA"
     gene            6112..6741
                     /locus_tag="PK898_08300"
     CDS             6112..6741
                     /locus_tag="PK898_08300"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HOU20070.1"
                     /translation="MKRSEPFSPRSKKSFQRPVNHKEVHQRILIVCEGTKTEPQYFKQ
                     FRVPGLKIQVEGTGKNTVSLVEYALQYRKDHEEENYDQVWCVFDKDDFPVADFEKAIR
                     LARKNKMRVAYSNQAFELWYILHFEYLNTSMDRKSYIKKLSDYLETEYKKNDDRMYHR
                     LRCKSDIAIKNAERLMKEYHPSHPGSNDPSTTVYELVKVLLNDSKLSSN"
     gene            6874..7218
                     /gene="rplS"
                     /locus_tag="PK898_08305"
     CDS             6874..7218
                     /gene="rplS"
                     /locus_tag="PK898_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOU20071.1"
                     /translation="MTDILRSIEAPINPNIPDLMPGDSVSVHVKIKEGNRERIQEFKG
                     IVLYTHNNGTNSTFTVRRVASNGIGVERTFLTRSPRIDKVVVERHSKVRRARLYYLRD
                     RSGKSARLKQKF"
     gene            7316..8050
                     /locus_tag="PK898_08310"
     CDS             7316..8050
                     /locus_tag="PK898_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOU20072.1"
                     /translation="MNRKPPLIAIVCNQELTNNPAEPRIDEVNTHYTRAILNQGGIPF
                     LIPVEFPLGDISLIFDMFDGLLLIGGEDVAIERFGGLPHESVSVPNLERDEIEIELTR
                     LAMAKDFPLLGICRGTQVMNVAMGGSLITDIPSQCPHALLHQRPKDAPSDSVPEHPVR
                     LLPNTMLRAIYETGSLKVNSYHHQAVDRLADCFRPAAYCEDGISEAYEAPDRRFVAGI
                     QWHPERMQDRPDQRKLFRAFVQACSA"
     gene            8062..9366
                     /gene="folP"
                     /locus_tag="PK898_08315"
     CDS             8062..9366
                     /gene="folP"
                     /locus_tag="PK898_08315"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOU20073.1"
                     /translation="METHRVFIGAGSNLGDRMENLTLAGQLLTPAVSILRTSPVYQTQ
                     PWGYSDQPDFYNLVWEAQTTLPPQSLLSYLKGIERKMGREETFRYGPRVIDLDILLFD
                     DLCYQSDNLTIPHPQMIKRRFVLQPLCELIPDEVDPQTGKDWRTLLAEAPEENVRKLP
                     FSLDLSRKVIRWGVKTYLMGIINLTPDSFSGDGLLQGAGDPLESALRKASAFLEEGAD
                     ILDVGAESTRPGFQSVTAETERERLLPVIRRLRERFPAALLSVDTCKAAVAKAALDAG
                     ADWINDVGGGLKDAAMTRVAAETGAPIILMRWQPLSEEAETMPQVLREAESLATHALS
                     AGVQRNRIILDPGIGFGTTPQQNLDILRDLAAFKKLGYPILLGTSRKSFIGHYLQKEV
                     DRRLMGTAATVTAGIVSGVDIIRIHDVQYLADVIRMTDLICR"
     gene            9397..10488
                     /locus_tag="PK898_08320"
     CDS             9397..10488
                     /locus_tag="PK898_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOU20074.1"
                     /translation="MAPEIRANQIFANDRKSDLTPNINNPYANFIKKRGAAMSIIPII
                     LAAGQGTRMKSATPKVLHPVMGKPMIDYILDEVAKVTSLRPIVVAGHGADQVIRAVEG
                     RADIAVQQEQLGTANAVAAAEKDAKGRAENVLVAYADMPCLRAETIRRVCDLQQQSAA
                     VFTMLTIIQEDSHGFGRIVRNADGSVAAIVEEAQASPQQLAIKECNVGLYCFQSEWLW
                     ESLKRVKLSPKGEYYLTDLVELANRDGKTVQAVVLEDREEALGVNTRVHLSEAEAIMR
                     KRVNTYWMTEGVTMIDPLTTYIDHSVRLERDVTLYPDTWISGETVIRSGSRIGPCTLI
                     ENSTIGRDCVICRAAVRNRQLEDHTEITG"
     gene            10589..11569
                     /locus_tag="PK898_08325"
     CDS             10589..11569
                     /locus_tag="PK898_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU20075.1"
                     /translation="MGNFIGCDLGGTNLRAAIVNSQTGTVSNLTIVPTLAREGYEEVL
                     LRMVDLFEHLIQESGLGKMNIDGIGIGIPGMMDVERGYTTFVTNLPGHWINIPVGPFI
                     TERIGIPTFILNDVRSITWGEYEFGAGKGCNSMVMYAIGTGIGGGVVINKQLVLGNSG
                     QAGEIGHMTVEPNGTLCNCGNHGCLEQYASGPAIRAAALKAIAHGATTILGEMVDYDL
                     NKMTPEVVFNAALKGDEIAKNIYETAGYYLGIAIGNSIVQLEPERIVIGGGIAQAGEI
                     LFAPMRRAIQEHVHLVPLERIQIVPAQLGNNAGVIGTSMWAEYMVRRKAL"
     gene            complement(11776..12471)
                     /locus_tag="PK898_08330"
     CDS             complement(11776..12471)
                     /locus_tag="PK898_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU20076.1"
                     /translation="MKKEIPTKKHLEKLAEYNLQTIPPEKWMSVRYQPGEAITREGVE
                     IENLALVIGGMAKVYRTAPNGRNLILCYYLSDGVIGEIELLTRRKRATSTVTAISEFE
                     CIAIDSEICAAELKTNIPFSNKLGTTLAEKLERSTDNYVLSALCSAEERLCTYILQNA
                     PTGLFRDVLTDVACSVGTSYRHVLRLLSELCSEGILRKSESGYEIMNREELIRRSRAT
                     ANQKSGIVKKELR"
     gene            12582..13481
                     /locus_tag="PK898_08335"
     CDS             12582..13481
                     /locus_tag="PK898_08335"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU20077.1"
                     /translation="MVQIAALLTGITLAVMISINGHLTAEFGIFPAAAIIHAVGSLFA
                     MILCAFQKPKKPLWGHQPLWIYLGGAIGVFTTVFNNLAFGRISMTSIVALGLLGQTVM
                     SLIVDSAGLLGMKKQPFLKSSIPGLIFALIGVFIMLDQTVTEAVIAVIVSFAAGVSVV
                     LSRSVNSRLAEKVGALRGSLVNHLVGLPVTILLAAASLRRAGDPFNIEFPIRPWIFLG
                     GVLGVTVVLLCNLTVSHISAFHLTLLTFIGQVFTGIWLDLSAGNGYSDVTFRSGLIIA
                     GGVILSRLTEWVTAKRFKQRSVS"
BASE COUNT         3555 a         3057 c         3492 g         3394 t
ORIGIN      
        1 ttccgggaaa tgaccgttcc ctattaccct gtaaccattt tttatgcgtt taaacaagca
       61 ggaaatcaaa acgaggaaag gcaaacggaa gaattttcat tggataacga gcgagtttcc
      121 accggatggg agaccatgtt aaatggcttg ttggcaagta gctttgaaat aaccggaacc
      181 tggccgatgc gcaccgagtt ggcaaatcgc agtgtcggaa taggagccaa tgcattggct
      241 tcctctattg ttttggtctg ccggccgcgg gcggaagatg ccccgtccgt aagccgccgg
      301 gaatttctgg cagcgctcaa tcgggaactg cccgccgctc tgcaccgcct gcaacgggga
      361 aatattgcac cggtcgattt ggcccaggcg gcgatcggcc cggggatggc gatcttttca
      421 cggtattcag cggtgttgga atcggacggt tcgccgatga gcgtccatac cgcattggcg
      481 ctgatcaacc ggacattgga tgaatacctg gctgaacagg aaagcgaata cgattcggat
      541 tcgcggtggg cattggcatg gttcgaacag ttcgggcatg aagccggacc ttatggaatt
      601 gccgaaacac tttcgaaagc caagaatacc ggcatcgagc gcttaaccca tgccggtatt
      661 ctggaagcca gaggcgggac agtccgtctc ttaagacggg atgaattggc aaaagactgg
      721 gacccgtccg ttaccaaacg ggtgacggta tgggaagcaa cccaatattt aatccgcgct
      781 ttggatgaag acggcgaaat cggtgccgga tcgctgttag gaaaactggc cgaatatggg
      841 gagaccattc gcgatctggc ctaccgtttg tatacactct gtgaacggaa gggatgggcg
      901 caggatgcaa cggcttataa catgctgatc atcgcttggc cccgcctgaa aaatctgtcc
      961 gataaccgaa cagaatccgt tcaggaaagt cttttataaa atctcggaag gaagataaaa
     1021 gaaaaagcgg gaggagacat ggctgtcagc aatatcgaaa atatcggaaa aggcttgaat
     1081 attttaaagg aaggattggc accttatatc cgacgggagc tgaaggaata ttacaaaggg
     1141 aactggtgga ccgacggaat cgaacgggca ctgcaatata atgctggttc taatgccctc
     1201 tcgggaaacg gttcaccgga tgagcgcttt gacttgctgg atatccaagc gcttctgatg
     1261 atcatgtggg aaaattggaa tgaggttttc aaaacagaac tggggcacac cggccgcagc
     1321 tatgtcagcg agttgcgcga aataagaaac aaatgggctc accaacagcc ttttgatgca
     1381 gatgaaacct ttcgggcttt ggatacaatg ctgttgctat tggaaatggt taatggatcc
     1441 ggacaggaag aacttcgggg tatgcaatat gagtttatgc ggaagaaatt tgacgccgaa
     1501 accaaacggg aactaaaaaa atcaacggaa aatctgtcaa acggtgtctc ggcgggattg
     1561 aaaccctgga gagagattgt cacgcctcat catgatgtcg cttcggggca ttatcaacaa
     1621 gccgaattcg ccgccgattt gtttcaagtg atcagcggta aagccgaacc ggaatatctg
     1681 gatccggtcg aattcttcag acggacctat ttaacggaag gattgagccg gcttctctca
     1741 caagcttgga taagattgac ttccgccggc ggagatccgg tagtagagct ccagacaaat
     1801 ttcggcggcg gaaaaaccca ttcgatgtta gctttgttcc atctgtttgg cggacagatt
     1861 aaacccgcac aagtaccgga actggaaaag ctggtccccg ccggattaaa aggcggtgaa
     1921 ttggatttgc cggtagcctc gcgggcggtt ttggtcggca cgcagctcag cccttcggaa
     1981 gtgagaacca aagcggacgg tactcaagtt catactttat ggggagaaat ggcttggcag
     2041 atcggcgatg ccaatggcaa agctcatgaa gcctatgaac ttgtcgcaaa ggaagaccaa
     2101 gacgggatca gtccgggttc cgaaaaactg gctgaattat tcgcacaatt cggacctgtt
     2161 ctggtattga ttgacgaatg ggtcgcttat acgcgccaat tatacggaaa ggaaaatctt
     2221 cccgggggat cgtttgattc caatatcaca tttgtacagg cattgaccga agccgcgaaa
     2281 gccgttccgg acacaatggt cgttgcggca ataccggaat cggatattga aataggcggt
     2341 gaaggaggaa aagccgcgct cgaacgaatt cagaatgtat tcggcagggt tgaatcggtt
     2401 tggaagccgg cttcctcaac ggaaagtttt gagattgtcc ggcgaagact gttccaaccg
     2461 atttcggaag cggattacgc ctcaagggat gcggtttgtc gtaaattcgc cgagatgtat
     2521 caggtcaacc gcgccgaatt tccaacggaa tgccgtgaaa tggattatga aaaaaggatg
     2581 attgccactt acccgattca tccggagttg tttgaccgcc tttacgaaga ttggtccact
     2641 ctcgaacgtt ttcaacgtac tcggggtgtt cttcgcatga tggcggcggt tatttatcgg
     2701 ctttgggata atcgggacaa ttcattacta attatgccat gtaccgtacc tttggactgg
     2761 atggatgtcc gttccgaaat cacgcgttat cttccggaag gttggggtgc ggtcatcgat
     2821 aaggatgttg acggccctca gagccagccg gtgaaactcg accgtgagaa tccgaatctt
     2881 ggtcgctact ctgcttgccg gcgggtagcc agaaccatct ttttcggcag tgctccctca
     2941 gccagttcga aaaaaatcca tgggatagaa gaagtccgca ttaagttggg gtgtgttcaa
     3001 cccggcgaac agccgtccgt atttggagat gcacttcggc gcttatccga acagttgact
     3061 tatctctctt ctgaatcaac ccgttattgg tttgatattc gaccgacaat caaaaaaatc
     3121 gcatccgaca gagccgccca atatgaacac aagcctgaaa ttgttgaaga tgaaattact
     3181 aaacggatga aatcttccgt tcgaaatgac cgaggtgatt tcgccggcgt acatgctttt
     3241 ccgaaaaaca gcggtgatgt acccgatgaa caagcatgtc gcttggtcat attgaatcct
     3301 tcctccgtac agcgaaaaca atccaaagaa agtgatgcaa tccaagctgc acaagatatt
     3361 cttgaaaata gaggaaatat tcctcgactc tatcgtaata tgcttatctt tctggctgca
     3421 aacggagatc gtctacccga tttggagaat gcggtacgga attggctagc ttggacttcc
     3481 attcaacaag atgaagagca attaaatctg gattctcacc aaaaacgcga agtggaaaat
     3541 caaattcgtg agtatgataa cagaattaat gcacaattgt ccgagacctt ctgcttttta
     3601 cttgttccga cgcaggaagg aaccgatccg gtcaactgga caatctaccg gttacaaaac
     3661 ggtgatcaat tggtaagcag ggcatccaaa aagttaaaaa tggaagagca attaattaca
     3721 gattgggcac cggctctctt gcgcagaggg ttggatcgtt ggctttggaa ggatgaaaat
     3781 catattcata tcgataagct atggggaaag ctggctcaat atctttacct tcccaggctg
     3841 agagatgaag aagtattaat caagacaatc cgaaacggcg tcaattccac tgattgggaa
     3901 gaatattttg cttatgcttc cggtttaaaa agcgacggaa cctatttggg tttaattgcg
     3961 gcggaaattc cctccgttgc tatcgatcat gaaagcttat tagtaaaacc cgaagtcgca
     4021 aaaaaacaac tcgaaaaaac gggaaaaacc cggttgggac aaggtgaata tgttaatttg
     4081 gccgaattat cacctaattc tgtcaacgat cgattagatt ttattggcag atcttccaat
     4141 gaaggggaat cggaacaggg gaaaaagact tttattcaca gatttcatgc cacagttgaa
     4201 ttagacccca agcgcctggg aagggatgcc agcaatattt cagaagaagt gttaactcat
     4261 ctaaccagta acgttgatac aaaagccacc gttacacttg agattgatat tgacgttccg
     4321 ggaggtatct cggaagataa aattcagatt attaaggaga actgtaatac actcaagttc
     4381 aaaaccgttg attttgagta aaaaatattg ataaacaata tcaatctaaa aaaaatcagc
     4441 tgcagatcat cggttcattt ggggaaattt acatcctgga aatagaattc tgcgacataa
     4501 tggattgatc gaacggattc ttccttctga ttatcggaat cagctggctc tacgatgttt
     4561 aatcaacctt atcaatatct gagtgcagat cgatccttgg tcgaagattc tttctccgat
     4621 gtgcctttta attttcctca gaaacagcaa cctgaatacc atagccgaca aaaagattgg
     4681 ttattttagc tgttaataaa ttataaattc catattgact ttacaaagta atgttatata
     4741 atgtgattat atgtgaaatt ttcacagatt tatcgtttat tttgcattac gaaggcggtt
     4801 ttatgttaat cgaatttact gtcgggaatt atcgctcatt caaagacccg gtaacactca
     4861 gcatggtcgc agcgaaatta aattcaaaat atcccgagat tgatgaaagc aatacttttc
     4921 aagccgagca gggtttgaca ctgctaagca gtgcagctat ttatggggcg aatgccagcg
     4981 gaaaaagcaa cttgattcgg gcatttcagt taatgagaga tttcattcaa aattcttcca
     5041 aagaatcatt ggcagatgag ccgattcata ccactccgtt tcgcttagat tcaaaagcag
     5101 agcacagccc ttcgtatttt gaagtcgttt tccgtttgga tgatatccgt tatcgatacg
     5161 gatttgaaat tacgacggaa aaagtggaaa cagagtggct gttttattct ccgaaaggga
     5221 aagaatcgaa attgttcacc cgtgaaggaa acgatatttc tttttctcgc gcttttaaag
     5281 gcaataaaac tgccctgata tctttaactc gtcccaatgc actttttctt tctgtcgctg
     5341 cacaatttaa taatgaaatt gccacaaaag ttctgggctg gttcctgtcg gttaacgtga
     5401 tttcgggatt ggatgatacc ggctatcgcg gattcactac tatggccttt gcaaagaata
     5461 aaacgtatcg tgaaaaaatt attcaattta tccgcggttt tgacgtcgga atcgatagta
     5521 ttattatcga aaaagcaaag atctctgaat cgagaatgtt tccaaaggat ctaccccaag
     5581 agttaaaaga ttttttctta aaacagataa aagagggtga tgagctgatc aattttagac
     5641 cgattcatcg gaagctttgc gaaaacggag cccttgagga aacgaaattt gatctcaccg
     5701 atgaatccga agggacacaa aaattgttct atttatccgg accgattatt gataccttgc
     5761 ttggaggaaa ggtcctgttt atcgatgaaa tcgaagcccg tttacatcct ttactgacgc
     5821 ggaaattaat cggcttgttt cactccaaag aattaaatcc gcgtcatgct cagcttatat
     5881 ttgcgactca tgatacaaac ctgctttcga acaagttgtt tcggcgtgat cagatttggt
     5941 ttgtagaaaa ggatgaagaa ggagcaagcc atctttattc tctggcggaa ttaaaggtac
     6001 gaaatgatgc atctttcgga agcgagtatt tacaagggag atacggagcc atcccgattt
     6061 tcggtgagtt taaagaaacg ttgatggatt ttgaacggga agatcatcaa gatgaagcgt
     6121 agtgaaccat tttctccgcg gtcgaaaaaa tcttttcaaa ggccggttaa ccacaaagaa
     6181 gtccaccaac ggattctgat tgtatgcgaa ggaacgaaga cagaacctca atatttcaag
     6241 caattcaggg ttccgggatt gaaaattcag gtcgaaggga ccgggaaaaa tacagtgagt
     6301 ctcgtagaat atgctttaca atatcgtaaa gatcatgaag aagagaacta tgatcaggtt
     6361 tggtgtgttt tcgataaaga tgattttcct gttgcagatt tcgaaaaagc cattcgtttg
     6421 gcgcgtaaaa acaaaatgcg tgttgcctat tcaaaccagg cttttgagct ttggtatatt
     6481 ttacatttcg agtatttaaa tacctcaatg gatcgaaaat catatataaa aaagttgagt
     6541 gattatttag agaccgaata taaaaagaac gatgatcgta tgtatcatag gctacgatgt
     6601 aaaagtgata tagccattaa aaatgcggaa agattgatga aagaatatca tccttctcat
     6661 cccggatcga atgatccgtc aacaactgtt tatgagctcg taaaagtttt attaaacgat
     6721 tcgaaacttt catcgaatta aggctgcatc aaaagacctg tccccgaaat attacaatcc
     6781 gctttacagg ttcgggagat catgctataa tcttgcttcg cgtcgtttgg gcgctgaaat
     6841 tgaagcagcc tttgcaagga ttggtattga attatgactg atattttgag aagcattgaa
     6901 gccccgatta acccgaatat tcccgatttg atgccggggg attccgtgtc tgtccatgtg
     6961 aaaattaagg aagggaaccg ggaacgtatc caggaattta aaggaattgt cctttatact
     7021 cataacaacg ggaccaactc cactttcact gtccgccgtg tggcatcgaa cggcatcggt
     7081 gtggaacgaa ctttcctcac ccgctccccc agaatcgata aagtcgtcgt cgaacgccac
     7141 agtaaagtac ggcgcgcccg cttatattac ctgcgtgatc gcagcggtaa gagcgcccgc
     7201 ttaaaacaga agttttagtc tttttaccgg attttttcgt taaatcgata ccctcaggag
     7261 tggatccatt cactcctgtt tttttattca aacccgattt cgaaaggacg gatgcatgaa
     7321 ccgcaaaccc ccgctgatag ccattgtgtg taatcaggag ctcacgaaca accctgccga
     7381 gccgcgaatc gatgaggtga atacccatta cacccgcgca attctcaacc aaggcggaat
     7441 tccttttctg attcccgtcg aattccccct cggggacata tcgttgattt ttgacatgtt
     7501 cgatgggcta ttgctcatcg gcggagaaga tgtggcgatc gaacggttcg gcggtctgcc
     7561 ccatgaaagc gtatccgttc ccaatttaga gagagatgaa atcgaaatcg agttgacccg
     7621 cctggcaatg gcaaaggatt ttccgctgct gggcatttgt cgcgggacac aggtgatgaa
     7681 tgtcgccatg ggcggatcgt tgattaccga cattccttcc caatgccccc atgcgcttct
     7741 ccatcaacgt ccgaaggatg cgccgtccga ctcggttccg gagcatccgg tgaggctgct
     7801 gccgaataca atgctgcggg cgatttacga aaccggcagt ctcaaggtca attcctatca
     7861 tcatcaggcg gtcgaccgtt tggcggactg ttttcgtccc gccgcatatt gcgaggatgg
     7921 aatttcggaa gcctacgaag cgccggatcg acgctttgtc gccggcattc aatggcaccc
     7981 ggaaaggatg caagaccggc ccgatcaacg gaagttattc cgcgcttttg ttcaagcctg
     8041 ttcggcgtaa gagtttgcct gatggaaacg catcgagtct ttatcggagc cggaagtaat
     8101 ttgggcgatc gaatggaaaa tttgacgctc gcgggtcagt tgttaacgcc ggccgtctcg
     8161 attctgagaa cttctccggt ttatcaaacg caaccttggg gatattcgga tcagccggat
     8221 ttttataatt tggtctggga agcgcagacg acgctgccgc cgcaaagcct gttgtcctac
     8281 ctgaaaggga tcgaacggaa aatggggcgg gaagagacgt tccgctacgg accgcgcgtg
     8341 atcgatctcg atattctgct gtttgatgat ctgtgttatc aatccgataa cctgacgatt
     8401 ccccatccgc agatgattaa gcgccgtttc gtgctgcaac ccctctgtga gctgattccc
     8461 gacgaagtgg atccgcaaac ggggaaagat tggcgaactt tgttggcgga ggcgccggag
     8521 gagaacgtcc gaaagctgcc tttttctttg gatttatcgc gaaaggtgat ccgctgggga
     8581 gtgaaaacgt acctgatggg cattattaac ctgacgccgg acagtttttc gggggacggg
     8641 ctgttgcaag gcgccggaga tccgctggaa tcggcactgc ggaaggcgag tgcttttttg
     8701 gaagaaggtg cggatattct ggatgtggga gcggaaagca cccgtcccgg ctttcaatcc
     8761 gttacggcgg aaacggaacg ggagcggctg cttccggtca tccgcaggct gagagagcgt
     8821 tttcccgctg cccttttgtc ggttgatacc tgcaaagcgg cggtggcgaa agccgctttg
     8881 gacgccggtg cggactggat aaacgatgtc ggcggcggat tgaaggatgc cgcaatgacg
     8941 cgggtcgccg ccgaaaccgg cgcaccgatc attctgatgc gttggcagcc tctctccgag
     9001 gaggcggaaa ccatgccgca agtcctgcgg gaggcggaaa gcttggcgac gcatgccctt
     9061 tccgccgggg tccaacgcaa ccgaatcatt ctggaccccg gtatcgggtt cgggacgacg
     9121 ccgcagcaaa atctggatat cttgagagat ttggccgctt tcaaaaaatt gggttatccc
     9181 attttactcg gcaccagccg aaaatctttt atcgggcatt atctgcaaaa agaggtcgat
     9241 cggcgcctga tggggaccgc ggcaacggta acagccggaa ttgtcagcgg cgtagacata
     9301 atccgaattc atgatgtaca atacctggca gatgtcatcc gtatgaccga tttgatctgc
     9361 cgctgacgtt tgccggcgga ggatggagaa ccgagcatgg cgccggaaat ccgcgcgaat
     9421 cagattttcg ccaacgatcg gaaatcggat ttgaccccaa acataaataa cccgtatgcg
     9481 aactttataa aaaaaagagg agccgctatg tcgattatcc cgattattct ggccgcgggg
     9541 cagggaaccc gcatgaaatc cgccacgccc aaagtgcttc acccggtcat gggaaagccg
     9601 atgatcgatt acattttgga tgaagtcgcc aaagttacct ctctccggcc gatcgtggtg
     9661 gccggtcacg gagcggatca agtgatccgg gccgtggaag gcagagcgga tattgccgtt
     9721 cagcaagaac agctcggtac cgcaaatgcc gtcgccgcag ccgaaaaaga cgccaaaggc
     9781 cgggcggaaa atgtattggt cgcctatgcg gatatgcctt gcctgcgggc ggagacgatc
     9841 cggcgggtct gtgatttaca acaacaatcg gcggctgtat ttacaatgtt aactattatt
     9901 caggaggatt cccacggttt cggccgaatc gtgcgcaacg cggacggaag tgtggcagcc
     9961 atcgtggagg aagcccaggc ttctccgcag cagttggcga tcaaagaatg taatgtgggg
    10021 ctgtattgct ttcaatccga atggctttgg gaatcgttga aacgcgtcaa actatccccc
    10081 aaaggggaat attatctgac cgatttggtg gaattggcaa accgggacgg caagacggtt
    10141 caagctgtgg tgttggagga tcgggaagag gctttgggag tgaatacccg cgtccacctt
    10201 tccgaagcgg aagcgattat gcgcaaacgg gtgaatacct attggatgac ggaaggtgtt
    10261 accatgatcg atccgctgac gacttatatc gatcattccg tgcgattgga acgcgatgtg
    10321 accctttacc ccgatacgtg gatcagcggg gaaacggtaa ttcgttccgg cagccgcatc
    10381 ggaccctgca ctttgatcga aaacagcaca atcggccgcg attgcgttat ctgccgggcg
    10441 gctgttcgca accggcagtt ggaggatcat accgaaatca ccggctgaac gacttttccc
    10501 ccgaaacgag tccgcatccg atcaaaaacg aagtttcggg tagaatcatc attaatcaat
    10561 ctgtatacaa atacttccgg aggatattat ggggaatttc atcggttgtg atttaggggg
    10621 cacaaatctg cgggctgcca tcgtcaattc gcaaacgggg acggtgagta acctgacaat
    10681 cgtccctaca ctcgccagag aaggttatga agaggtcttg ttgcggatgg tcgatctgtt
    10741 tgaacacctg atccaagaat ccggattggg aaagatgaat atcgacggca tcgggatcgg
    10801 tattcccgga atgatggatg tcgagcgcgg atataccact tttgtgacca acctgccggg
    10861 gcattggatc aatatcccgg tcggtccctt tatcaccgaa cgaatcggaa ttcccacttt
    10921 catcctcaac gatgtgcgtt cgatcacctg gggggaatac gaattcggcg cgggcaaagg
    10981 ctgcaattcc atggtgatgt atgcgatcgg taccggcatc ggcggcggtg tggtgatcaa
    11041 taaacaattg gtgctgggga attccgggca ggccggagaa atcggtcata tgaccgtcga
    11101 acccaacggc acgctctgca actgcggaaa ccacggatgc ttggagcaat atgcatccgg
    11161 tcccgccatt cgagccgccg ctctgaaagc aatcgcgcac ggcgcgacga cgattttggg
    11221 agagatggtc gattacgact taaataagat gacaccggaa gtggtcttca acgcagcgtt
    11281 gaagggtgat gaaatcgcga aaaacatcta tgaaacagcc ggatattacc tcgggatcgc
    11341 tatcggaaat tccatcgtcc aactggagcc ggaacggatc gtaatcgggg gcggcattgc
    11401 gcaggccggc gaaatcctgt ttgcgcccat gcgcagagcg atccaagaac atgtccattt
    11461 ggtcccgctc gaaaggatcc agattgttcc cgcccaattg gggaacaacg ccggagtgat
    11521 cggcacgtcc atgtgggcgg aatacatggt ccgccgcaaa gcgctctgag ttttcctcaa
    11581 accgccgttc gggtataaaa aatcccggct gttcgaatca caacaaccgt cttcgatcgg
    11641 gttcattttc tttttcagcc ttcggcgagg ggatttttta cgggtttcga tcatccgatc
    11701 cggcgggaaa aaccttttcg gacagaactt gcgatatttc cggaattttg aacggcacct
    11761 tcattcggct gccggttagc ggagttcttt cttgacgatt ccgctttttt gattggccgt
    11821 tgcccgagaa cggcggatca gctcttcgcg gttcataatt tcatatccgc tctcggattt
    11881 ccgtaagatc ccttcgctac aaagctcgga cagcagacga aggacatgac gataacttgt
    11941 accgacggaa caggctacat cggtcaatac atcgcgaaac aagccggtgg gagcgttctg
    12001 cagaatatag gtacaaagac gttcttcggc agagcacaac gccgacaaca cataattatc
    12061 ggtgcttcgt tctaactttt ccgccaacgt tgtcccaagc ttgttcgaaa acgggatatt
    12121 tgttttcagt tccgcggcac agatttcact gtcaatcgcg atgcattcaa attcggagat
    12181 agcggtaacg gtgcttgtgg cacgtttccg ccgggtgagc aattcgattt ccccgatcac
    12241 tccgtcggaa aggtaataac aaaggatcag attcctgccg ttcggtgccg tgcgataaac
    12301 tttcgccatt ccgccgatga ccagtgccag gttttcgatt tcaacacctt cccgggtgat
    12361 ggcttcaccc ggctgatagc gtacactcat ccacttttcc ggcgggatcg tttgcaggtt
    12421 atattccgcg agtttctcca gatgtttttt ggtcgggatc tcttttttca tatcttttcc
    12481 cctttctctc tattagatca gaaaacgcaa aaaacaccat gacaaatgtc ctactccctt
    12541 cgctgcttat ttgatatgct ttcgattagg gacacagaaa aatggttcag atagcggctc
    12601 ttttaaccgg cattacgctt gcggtcatga tatccataaa cgggcatttg accgcagaat
    12661 tcgggatatt tccggcggcg gcaattatcc atgcggtcgg gtctctgttt gccatgatcc
    12721 tttgcgcttt tcagaagccc aaaaagccgc tctgggggca tcagccgctt tggatttatt
    12781 tgggcggagc gatcggtgtc tttaccacgg tgttcaataa cttggcattc gggcgcatca
    12841 gcatgaccag cattgtcgca ttgggattgc tgggacaaac cgtcatgtcc ctgatcgtcg
    12901 attccgccgg cctgctcggg atgaaaaagc aaccgtttct gaagagttcc attcccggat
    12961 tgatttttgc gctgatcggc gttttcatca tgctggatca gacagtcacg gaagcggtca
    13021 ttgcggtgat tgtttcgttt gccgcgggtg tttccgtcgt gctttcccgt tcggtcaact
    13081 cccgattggc ggagaaagtc ggagcgttac gtggttcgct ggtaaatcat ttggtgggcc
    13141 tgccggtcac aatcctgttg gcagcagctt ctttgaggcg tgccggcgat ccattcaaca
    13201 ttgaatttcc gatccgccct tggatttttc ttggaggggt tctgggggtg acggttgtgt
    13261 tgctttgcaa tttaaccgtt tcgcatatct ccgcttttca tctgacccta ctgacattta
    13321 tcggacaggt atttaccggg atctggttgg atttatcggc gggaaacggt tattcggatg
    13381 ttaccttcag gagcgggctg ataattgcgg gcggagtaat tctcagccgg ctgacggaat
    13441 gggtcaccgc caaaagattc aagcaacggt ctgtgagctg agagcagatt cagcgaaa
//
LOCUS       DAOSMM010000061        12770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4902_length_12770_cov_15.260322, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000061 DAOSMM010000000
VERSION     DAOSMM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4902_length_12770_cov_15.260322"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1931
                     /locus_tag="PK898_08340"
     CDS             150..1931
                     /locus_tag="PK898_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOU20078.1"
                     /translation="MQYEAIVHRSSEEFIYPVSRSSLKIRLLAKRQDLVSCEICYWNR
                     YTNLLKTQPLRVVYRDAYHDDFRTRLDFDGVARFMRYYFKLTDTNGQVCFFNEYGFTG
                     EPQKNGFFEHHYTNENEVIRRPDWAKGIIYYQIFPERFARGINQKSRHPYVDWNSRPA
                     LDNYFGGDLPGIIQKLDYLANLGIECIYLNPIFHADCNHKYATIDYFSVDPDFGTNDD
                     LIRLVKKAHDLGIRVILDGVFNHCGNNFPPFVDFRMNGKASPYADWFLPTDFNAEGTL
                     SRYEGFGDLSLMPKLRTGTPAVREMILKVMTYWVETAGIDGWRMDVADEVETQSWQVI
                     CAAFKHLYPDKLLLAETWTDANRLISYGDRFDCAMNYAFRDALSDFFAKRRIDSFGFD
                     HRINHLLSKYNEEVTPLMYNLIDSHDTPRFLTESGEDKRRLKLAVVFQMTCLGSPAIY
                     YGDEIGMEGNDDPDCRGGMKWDPKDQDHDLLSFYKTLISLRKREKALKSGRYVSILCE
                     PNGIYGFQRKHENETVYVIINNSDKEIAVELPVNIPFHPFRDLINHELFESIPLSEDD
                     KIWNNDIAVQNGKIRMKLPAMGFRILK"
     gene            2041..2616
                     /locus_tag="PK898_08345"
     CDS             2041..2616
                     /locus_tag="PK898_08345"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014483815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOU20079.1"
                     /translation="MKLLEDRILRDGVVKGGNILKVDSFLNHQIDVRLYDEIGKELYR
                     LFKDEPVTKVLTIESSGIGLAVAAAREFDVPALFAKKNRTKNIDGEVYSSAVESFTTG
                     ITYTIIVSKHFLGPQDHVLIVDDFLANGNALLGLIEIVEQSHAKLVGCGIAIEKGFQS
                     GGKMIREKGIRVESLAIIDKMDENGIVFRPQ"
     gene            2740..4266
                     /gene="guaA"
                     /locus_tag="PK898_08350"
     CDS             2740..4266
                     /gene="guaA"
                     /locus_tag="PK898_08350"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU20080.1"
                     /translation="MTHQTVLILDFGGQYKELIARRVRECNVYSEVYPGEISIDKIKA
                     LHPIGIILTGGPYSVYAQNAPKPDPELFRLNIPILGICYGAQLIAQMNGGRVEPCLIS
                     EYGQRKIIIARDSLLFEGLDKEQIVLMSHTDQIRELPEGFLATAFSETCPNVAIESVE
                     RSLYGVQFHPETELTIHGKQIIRNFLYRICHAEGDYTLENFIGEQIRTIRKQVGDGKV
                     ILGLSGGVDSAVCAALLAEAIPGQVICIFVDHGMMRKNEGDEVEKAFAAKSLEFVRIN
                     AADRFLSKLAGVTDPEQKRKIIGNEFVQVFSEQAALHNDAEFLAQGTIYPDVIESGSG
                     KAATIKSHHNVGGLPKDLNFKGIVEPLRSLFKDEVRQLGSALGLPQSIVNRQPFPGPG
                     LGVRIIGEVTREKLQILREADAIFREEIEADCVPADQYFAVLTNLRSVGVMGDSRTYD
                     YTLALRAVITHDFMTAEYAPIPHATLSRLSKRITNEVKGINRIVYDITDKPPATIEWE
                     "
     gene            complement(4491..6839)
                     /locus_tag="PK898_08355"
     CDS             complement(4491..6839)
                     /locus_tag="PK898_08355"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOU20081.1"
                     /translation="MKELKNPLRGRITVLLILLVAYFIATLYHSELWCNILSPLSALA
                     AGEILVFSYRKSDRSIRVRKAFLLLASGCFVWAIADILWAIIVFQGRDPESNPVIWIV
                     YALTNLLFLITLLYFAILQFQKWDLVQFFVDLVISGYLTALTFWIIFVQKNTEILYAL
                     LALDYASVFCILSDLLICISLVSWFLSIRSGKVPSFLRIISFGLILFAITDLYYYYAN
                     YNDIYLPNTLIDFIYMTSLYIIAFGGWWKVYKKSTLYELAVIMNTGGKTRWLSLLIYP
                     SVTFVIVYSGISDLHLSVIDFFAFAIPIFFYWGTCKYIQVSIEKEAILKQQNKILEQK
                     VSEQIDELTFLANQDTLTTLYNRRHFMSYMEDTLHSLGKDSIIAVLLIDLDRFKTIND
                     IYGHDVGDEVLVDLSQRMIEWNRYGATIARLGGDEFALMFAGKYSQEDIENFCEQLIA
                     LCREPIQVGDTQMNLTMSIGVALAAPDVHDGKTLLKRADIAMYHAKLQGYNKYQFYNA
                     SIDKDFKKLAKVESLLRQVDVEKDFKLFYQPQFSLPDLKLIGAEALIRWENAEMGFIP
                     PNVFIPIAEEIDYILKIGTWVMEETISQIKKWNTHYPVQLKVGFNLSPKQFKDRGFVD
                     LLETLISSANLDPECVDVEITESMMIDDETYVKNIFALLKKLGVSISIDDFGSGYSAL
                     NYLNKYPFDRIKLDKSLIDDLSTEKSNSIHIVQAAINMAHASGIQTIAEGVETKEQLD
                     ILIDLGCDQVQGFLLGRPVPPNIFEEKFLIRNFRYNEFPQNQ"
     gene            7503..8270
                     /locus_tag="PK898_08360"
     CDS             7503..8270
                     /locus_tag="PK898_08360"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOU20082.1"
                     /translation="MSDLFDLTGKVAVVTGASSGLGSQMAKALARQGADVAILARRVE
                     KLNQVKAEIEKMGVRCLAIQCDVLVNSEITKAVAAIKEHFGKIDILVNNAGIGMAAPA
                     EEMTDEMWRKVIDTNLNSHFFFSREVAKIMIENHYGKIINIGSIHSTVAMSGLPVNAY
                     ATSKGGILMMTKALAVEWAKYNITVNAIGPAYFGSEMTAELIDNPAFAQVLKTYCPMG
                     RAGKEGELDGAVVYLASDASSFTTGQILNVDGGWTAI"
     gene            8487..9182
                     /locus_tag="PK898_08365"
     CDS             8487..9182
                     /locus_tag="PK898_08365"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOU20083.1"
                     /translation="MPEKKSNAKIKITKNGPYLVSGNIPIHEKIIVPTGHTYKLVEGR
                     ELPQAENYALCRCGKSKNPPFCDGSHLIEPFNGKETASREPYTDRSELLEGPGVDLLD
                     DGRCAFARFCHRKNGTTWDYVLNSNTEEAISEAIQAAGECPSGRLTAVTKEGELSEPD
                     YDPEIDIVEDPERGVSAGIFVKGKIPIESEDGEIYELRNRVALCRCGHSRNKPFCDAS
                     HVPFGFSDGHVPE"
     gene            9379..10692
                     /locus_tag="PK898_08370"
     CDS             9379..10692
                     /locus_tag="PK898_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOU20084.1"
                     /translation="MDLLIAFLVLIISVLFSLFQNFSLLIPLGIGLICFISVGLYRHY
                     PLRSLFKMALVGAKNSLVVIRVLVLIGCLTALWRACGTISFFVVFGVKLITPHIFILV
                     AFLLSSLVSYAMGTSFGVAGTVGVILMSIARAGGVNEYIAAGAILSGVYFGDRTAPTS
                     SCANLVASLTKTDLYDNVKRMLKSAALPFVICIFLYAGLSWKNPLSSADTSLVSVLEN
                     NFSLSVWTLIPAVILLILPLFRIPVRIAISISILFSFAVSMGSQQMGFEDTFHAMLFG
                     YKPSDPTLTGILSGGGLIPMLTTILIVALSSTYSGIFNGTNMISGIREKLFDLSEKIG
                     RFPTVVATSILSCAVFCNQTMGIIFTHEMVGKIYQEKKIDRSTFMLDIANSTTLIAVL
                     IPWSIASSVPLTWMGVNFKALPYAFLLYLIPLFYGIQEQHKSKQH"
     gene            10809..11234
                     /locus_tag="PK898_08375"
     CDS             10809..11234
                     /locus_tag="PK898_08375"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HOU20085.1"
                     /translation="MPWTTKNYPNSWKNFDPLLRKKAIDIANAMLADGYKEEDIIPIA
                     TSQAQKWYENATPAELKELEEKDVTKHAKPKGPSGARLMEKDVIVRYQPEQKQWEVIS
                     DGALQADSLHHTKKEAVIRAREIAGKRGTRVIEKKKSES"
     gene            11539..>12770
                     /locus_tag="PK898_08380"
     CDS             11539..>12770
                     /locus_tag="PK898_08380"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOU20086.1"
                     /translation="MALAVFLFVLTYILLLALPKYRAYVALVSAILFIGTGIISPVKA
                     VEGIDWNVLMMITGTMGTVYLFCQSRMPEKMADVILERMPDVKWVIVSLALFAGIISA
                     FVDNVATVLMVAPIALTVAKRLKISPVPIIIAIAVSSNLQGAATLVGDTTAIMMGSQA
                     GMDFIDFFFFHGRPSMFWFTEVGAVVSALILLWQFRDKNEKVTDVLNTEVNDYVPSIL
                     LFGTVLLLIVASFINPKPKITNGLICMSIMLIGIVFEGIKNHGLADSFRETFGSIDFF
                     TLLLLASLFMVIAGIKEAGVIDSLAALFVRLSGNSLILIYSLILWGSVLISAFIDNIP
                     YVATMLPVVAGIASYMQIDPTLLYFGLLSGATLGGNLTPIGASANITGIGILRKNGYE
                     PSTQDFMKISVPFTLSAVT"
BASE COUNT         3698 a         2635 c         2751 g         3686 t
ORIGIN      
        1 gttccgcaat agtataccac aattcacagc gaatcgaaaa ttattgattc ctctttcccc
       61 tgccaaatca tcagatcgtc ataactttag ctcccgatcc ataatcactg tcataataag
      121 gacgatttat gacagattaa aggatatcta tgcaatacga agcaatcgtt catcgaagtt
      181 ccgaagaatt tatttatccc gtcagccgat cgagcctgaa aattcgcttg ctggcaaaac
      241 ggcaggactt ggtttcttgt gaaatctgtt attggaatcg ttacacaaac cttttgaaga
      301 ctcagcctct gcgggtggtt tatcgagacg cttatcatga tgattttcgg acccgactcg
      361 attttgatgg ggttgctcgt ttcatgcgat actattttaa actgaccgat accaacggtc
      421 aggtctgctt ctttaatgaa tacgggttta ccggtgaacc gcaaaaaaac ggcttctttg
      481 agcatcacta tacgaatgaa aatgaagtga tccgaaggcc ggattgggca aagggaatca
      541 tctattacca gatctttccc gaacggttcg cccgcgggat taatcaaaaa tcacgccatc
      601 cttatgtaga ttggaacagc cgacctgctt tggataatta ctttggcgga gatttgccgg
      661 ggatcattca aaaattggat tatctggcaa atctcggaat tgaatgcata tatttgaacc
      721 cgatttttca tgcggattgt aaccataagt atgcgacgat tgattacttc tccgtcgatc
      781 ccgatttcgg gacgaatgac gatttaatcc gtctggttaa gaaagcgcat gatcttggga
      841 ttcgtgtgat tttggatgga gtgttcaatc attgcggaaa taattttccg ccttttgtcg
      901 atttccgaat gaacgggaaa gcttctccgt atgcggattg gtttctgccg acggatttta
      961 atgcggaagg aactttgagc cgttacgaag ggttcggcga tttatccctt atgcctaaat
     1021 tacgtaccgg cacgccggct gtccgagaaa tgatcttgaa agtaatgacg tattgggtcg
     1081 aaactgccgg catagatgga tggcggatgg atgttgcgga tgaagtcgaa acgcaatcct
     1141 ggcaggtaat atgtgcggca tttaaacatc tctatcccga taaactttta ttggctgaga
     1201 cttggacaga tgcgaatcgc ttgatcagtt atggtgaccg atttgattgc gcaatgaact
     1261 atgcttttcg ggatgcttta tcggattttt ttgccaagcg cagaatcgat tctttcggat
     1321 ttgatcatcg aatcaatcac ctcctttcta aatataacga ggaagttaca ccgctgatgt
     1381 ataacctgat tgacagccat gacacacccc gttttttgac cgagtcggga gaagacaaac
     1441 gtcgtttgaa acttgcggtt gtctttcaaa tgacatgcct cggatcaccg gcgatttatt
     1501 acggtgacga aatcgggatg gaagggaacg atgatccgga ttgcagaggc ggaatgaaat
     1561 gggatccaaa agatcaggat catgatttat tgtcttttta taaaacgttg atttctttac
     1621 gaaagcggga aaaagcgttg aaatcaggca ggtacgtttc tattctttgc gagccgaacg
     1681 gtatatacgg atttcaacgg aaacacgaga atgaaaccgt ttatgtaata atcaataatt
     1741 cggataagga aatagcagtc gaactgcccg ttaatattcc ttttcatccc ttccgtgatt
     1801 taatcaacca tgaattgttc gaatcgatcc ctctttccga agatgataaa atttggaaca
     1861 atgatatagc cgttcagaac ggtaaaattc ggatgaaact gccggctatg ggtttcagaa
     1921 ttttaaaata agaatcagca ttttcctttt ccttccgaag tgattcagac aaatgctttt
     1981 ataataggca ttcatccgtg tattaaattt aacttgttat tcagataggg gaggtatgtc
     2041 ttgaaattat tggaagatcg aatcttaaga gacggcgtcg taaagggcgg caatatctta
     2101 aaagtcgaca gctttctgaa ccatcagatt gatgtccggc tttatgatga aatcggaaaa
     2161 gaactttatc gactttttaa ggatgaaccg gtcacaaaag tcctgaccat cgagagttcc
     2221 ggaatcggtc tggctgtcgc agctgcccgg gaatttgacg ttcccgcact atttgcgaaa
     2281 aagaatcgaa caaaaaacat cgacggagaa gtctattcct cggcggtcga atcgtttaca
     2341 accggaatta cctatacgat tatcgtctcc aaacattttc tcggaccgca ggaccatgtg
     2401 ctgatcgtgg atgattttct ggctaacggc aatgcccttt taggcctcat agaaattgtt
     2461 gaacaatccc atgccaaatt ggtcggttgt ggcattgcta tcgaaaaagg tttccaaagc
     2521 ggcggaaaaa tgatccggga aaaaggaatc cgagtcgaat ctttagccat tattgataaa
     2581 atggatgaaa acgggattgt cttccgtccg caataattta ttctgagaag acggttttca
     2641 atcgatggtt caaattttgg gcaacagagt caaacctatc cgttgttcaa atttctttcg
     2701 aaaacgccgt ttttatttac tgacaaggaa tacagattca tgacgcatca gactgtttta
     2761 attctcgact ttggcggtca atataaagaa cttatcgccc gccgtgtacg cgaatgcaat
     2821 gtttattcgg aagtttatcc cggcgaaatt tcgatcgaca aaattaaagc actccaccct
     2881 atcggtataa ttttgaccgg cggtccttac tcggtctatg cgcagaatgc gcccaaaccg
     2941 gaccccgaac tcttccgcct caacatcccc attctcggta tctgttatgg cgcacaactg
     3001 atcgctcaaa tgaacggcgg tcgggtcgaa ccctgcttaa tcagcgaata cggtcaacga
     3061 aaaattataa tcgcgagaga cagtctgctt tttgaaggct tggacaagga acagatagtc
     3121 ctcatgagcc acaccgatca aattcgggaa ttaccggaag gttttcttgc aaccgcattt
     3181 tcggaaacct gcccgaatgt tgcgattgaa tcggtcgaaa gatctcttta tggagtacag
     3241 tttcatcccg aaaccgagct cacgattcac ggtaagcaaa taatccgaaa ctttctttat
     3301 cgaatctgtc acgccgaagg tgactatacg cttgagaatt ttatcggaga acagatccgg
     3361 accattcgaa aacaggtcgg ggatgggaaa gtgattctcg ggctttcggg cggggttgat
     3421 tcggcggtct gtgctgcctt gttggcagaa gcgattcccg ggcaggtaat atgtattttc
     3481 gtcgaccatg ggatgatgcg gaaaaatgaa ggggacgaag tcgaaaaagc gtttgccgca
     3541 aaatcgttgg aattcgttcg aattaacgcc gctgaccgtt tcctgtcaaa attagcgggt
     3601 gtcaccgacc cggaacaaaa aagaaagatc atcggtaatg aatttgttca ggttttttct
     3661 gagcaggctg ctctgcataa cgatgccgaa ttccttgctc agggaacgat atatccggat
     3721 gtaatcgaaa gcggcagcgg gaaagcagcg acaatcaaaa gccaccataa cgtcggcgga
     3781 cttcccaagg atctcaattt caaaggaatt gtcgagccgt tacgctctct ttttaaggat
     3841 gaagtccgac aactcggatc cgctctggga ttgccgcaat cgattgtcaa ccgccagcct
     3901 tttccgggac ccggattggg cgtgcgaatt atcggtgaag tcacccgcga aaaattgcag
     3961 atcttgagag aagcggatgc gatattccgt gaggaaatag aagcggattg tgttccggcg
     4021 gatcaatatt ttgcggttct cactaacctg cgttctgtgg gcgtaatggg agacagccgg
     4081 acatacgact atacgctggc attacgcgca gtgatcacac atgacttcat gaccgcggaa
     4141 tatgccccca ttccccacgc cacactttct cgcctctcca agagaattac aaatgaagtc
     4201 aaagggatta atcggatcgt ttatgatatc actgacaaac cgcccgcgac gatagagtgg
     4261 gaataaaaaa ggcgtttcaa atttctatag gcaagtgaaa ataaaaaaaa ggaagttcca
     4321 acaaaattgt tattttgcac ccaaaataga aaaggacttc ccgtgataaa agatgtcaga
     4381 atgagtataa cgatcgaaat cgaaaatagc cggaggataa gatttgaaca tgcggttcaa
     4441 tctgacaact tcggacaaag gataagaaat gtccaaaggt aaattcgatt ttattgattt
     4501 tgcgggaatt cgttataacg gaaatttcta attaaaaact tttcttcgaa tatattggga
     4561 ggaacaggtc ttccgagcag gaatccctga acttgatcac atccgagatc gatcagaata
     4621 tccagttgtt ccttggtctc gacgccttcg gcaatcgttt gaatcccgga tgcgtgtgcc
     4681 atgttgatag ccgcttggac aatgtggata ctgttggact tttccgttga caggtcatcg
     4741 attaatgact tatcgagttt aatcctgtcg aaaggatatt tattcagata atttagagcg
     4801 gaatatcctg acccgaaatc atcaatcgaa atcgaaacgc ccaatttttt aagtaacgca
     4861 aagatatttt tcacataggt ttcatcgtca atcatcatgc tttcggtaat ttctacatcc
     4921 acgcactcgg gatcaagatt cgccgaagaa attaatgttt ccaacagatc gacaaaacct
     4981 ctatctttaa actgtttggg agaaagattg aaaccgactt tcagctgcac cggatagtga
     5041 gtattccatt ttttgatctg gctgattgtc tcttccatta cccacgtacc gattttcaga
     5101 atatagtcaa tttcctcggc aatcgggata aacacatttg gcggaataaa acccatttcc
     5161 gcattttccc agcgaatcag cgcttcagct ccgattaatt tcagatcagg caaggaaaat
     5221 tgaggttgat aaaacaattt gaaatccttt tcaacatcga cctgtcgaag cagcgattcg
     5281 actttagcaa gctttttaaa atctttatcg atcgaggcgt tataaaactg atatttattg
     5341 tatccctgta acttggcatg gtacatcgca atatccgcgc gtttgagcag cgttttcccg
     5401 tcatgaacat ctggagcagc cagtgcgact ccgatgctca ttgtcagatt catttgagta
     5461 tcaccgacct gaatgggttc acggcataaa gcgataagct gttcgcaaaa gttttcgata
     5521 tcttcttgag aatattttcc cgcaaacatc aaagcaaatt catcgccgcc caacctggcg
     5581 atcgttgcac catatcgatt ccactctatc attctctgcg ataggtctac taaaacctca
     5641 tctcctacgt catgaccata aatatcgttg attgttttaa agcgatccaa atcgattaac
     5701 aaaacagcta ttatgctgtc tttgccgaga gaatgtagtg tatcctccat ataagacata
     5761 aaatgacgcc ggttataaag cgttgtcaac gtatcttggt tggcgagaaa agttaattca
     5821 tcaatctgtt cggatacttt ctgttccaaa attttattct gctgctttaa aattgcttcc
     5881 ttttctatag agacttggat atatttacat gtcccccaat aaaaaaatat gggtatcgca
     5941 aatgcgaaga aatcgatcac gctcaaatgc aaatcggaaa tacccgaata aacaatgaca
     6001 aaagtcactg atggatatat tagaagagat aaccatcgtg tcttaccgcc tgtattcata
     6061 attactgcga gctcatacaa agtacttttt ttatatactt tccaccaacc gccgaatgcg
     6121 ataatatata acgatgtcat ataaataaaa tcaattaagg tatttggtaa atatatatca
     6181 ttgtaatttg cataataata gtatagatcc gtgatcgcaa acaaaatcaa tccgaacgaa
     6241 ataattcgca ggaaggacgg aacttttccg gaacgaatcg ataggaacca agaaacgagg
     6301 ctgatacata tcaaaagatc ggaaaggata caaaaaacac ttgcataatc caaagcaagc
     6361 aacgcatata aaatctcggt atttttttgt acgaaaataa tccaaaacgt cagggcagtc
     6421 agataaccgc tgataaccag atcaacaaaa aattgaacga gatcccattt ttgaaattgc
     6481 agtatcgcaa aatagagtaa cgtaatcaga aaaagtaaat ttgtaagcgc ataaacaatc
     6541 caaataaccg gattactctc gggatctctt ccctgaaaga caataattgc ccaaaggata
     6601 tcggcaatcg cccaaacaaa gcagccggag gcaagtaaaa gaaatgcctt ccttacccga
     6661 atagaacgat ctgattttcg ataactaaaa acaagaattt ctcccgctgc taatgcgctg
     6721 agtggggata aaatattaca ccacaattcc gaatgataca aagtggcgat aaaatatgcg
     6781 acgagaagaa tcaataaaac agttattctt cctcttaatg gattttttaa ttctttcatc
     6841 gtcttttcct ttttttattt tcgaaaatca ataaaagttc agttaaccta ataatagcga
     6901 ttttatcgta aattacaatt tcaagtgatt attagcactt ttttattatg attaactaca
     6961 ctatttattg acaagcgttc acactcgatt gctttaagtt tcagtgggtc agtttgtaat
     7021 taaaaggaac gccgtctatt ttgtgtttgt ctttttattg gaaataattt taggtgattt
     7081 tgttctatag aatcagcgga ttgatttccg ggttgattca gatatttcca tttaccgaat
     7141 ttatcgaaat taccgaaaaa tattatatcg aagaattcaa tttttcagtt gaaatcgata
     7201 taaaatcttt ttatcgatcg cttctgattt atggagtttt ataataaata aaccctcatt
     7261 ttccatctta aaacatccaa aaaaccaaat tttggctcaa atcaatatcg ttttttatct
     7321 gttttataga tctttcccga acattcctat aattgaccgc ataaataatt gtgatatatt
     7381 taacaatgct caaatacata ctctttcaga ggaccggatg aaagaacggt tccaataaag
     7441 agaatggaaa aaggaaaaga aattttatat ttttgtaaaa tttgaaagga atttatcgta
     7501 ttatgtcaga tctgttcgat ttaacaggaa aagttgctgt cgttaccggt gcctcatcgg
     7561 gtttgggatc tcaaatggca aaggcgttag ccagacaagg tgcggatgtt gcgattcttg
     7621 ccagaagagt tgaaaaatta aatcaggtga aagccgaaat tgaaaaaatg ggcgtccgct
     7681 gcctcgcaat ccaatgcgat gtattggtta attccgaaat taccaaagcg gttgccgcta
     7741 tcaaagagca ttttggcaaa atcgatattt tagtcaacaa cgccggtatc ggaatggcgg
     7801 cacctgcaga agaaatgacg gatgaaatgt ggcgaaaagt gatcgacaca aacttaaaca
     7861 gtcatttctt cttctcccgc gaagtcgcga aaatcatgat cgaaaatcat tacggaaaga
     7921 tcatcaacat cggttcgatt cacagcacag tcgctatgag cggtttaccg gttaacgcct
     7981 atgcaacttc gaagggcggt atattaatga tgaccaaagc gctggctgtt gaatgggcaa
     8041 aatacaacat caccgtcaat gcaatcggac cggcatattt tggcagtgaa atgaccgccg
     8101 aactgatcga taacccggcg ttcgctcagg tactgaagac ctattgcccg atgggcagag
     8161 ccggaaaaga aggtgaattg gacggagcgg tagtttacct ggcatcggat gcttccagct
     8221 tcacaaccgg tcaaatccta aatgtcgacg gcggttggac ggctatctaa acagatttcc
     8281 gaaaggaaac aaatctatcg gataaaaaag aagcggttgc ctaaaaagca actgcttttt
     8341 ttcatccgaa actcagagaa cagcgtttcg atgccacatc ggataatatc tggaattgtc
     8401 tttaatccgg ctaagacttt acgcggcatt ataatcagaa gcgttcaatt aacgttttga
     8461 gatgtgaatg aaaaggatga ttttatatgc cggaaaagaa atcgaacgcc aaaattaaaa
     8521 taacaaagaa cggtccctat cttgtatcgg ggaacatccc gatccatgag aaaatcattg
     8581 ttcccaccgg gcacacatac aaattagtcg agggaagaga gttgccgcag gcagaaaatt
     8641 acgcgctttg ccgttgcggg aaatcaaaaa atccaccttt ctgtgacggt tctcatctga
     8701 tcgagccttt taatggaaaa gagaccgctt cccgagaacc ttatacggat cgttccgaat
     8761 tactcgaagg accgggagtt gatctgctgg atgacggcag atgtgccttt gcccgatttt
     8821 gtcatagaaa aaacggaacg acttgggatt atgtgctgaa ttcaaatacg gaagaggcaa
     8881 tcagtgaagc gattcaggcc gccggtgaat gcccctccgg aagattaacc gccgtaacca
     8941 aagaaggcga gttgtcggag ccggattatg atccggaaat cgatatcgtt gaagacccgg
     9001 aaaggggtgt gagtgccggc atctttgtga aaggaaaaat ccccatcgaa tccgaagacg
     9061 gtgaaattta tgagctaagg aaccgagttg ccttatgcag atgcggacat tctcgaaaca
     9121 aacctttctg cgatgcctcc catgttcctt tcggattttc agatgggcat gttccggaat
     9181 agtcttttgg aagcgaacga gaaatgcatt cgataaaaaa agtatccgga aaaatccttt
     9241 tacttgattt tcccgtcata agttccataa aacgggttcg atgatcgata catttctgag
     9301 tcgatacgaa aggaaacgag tcaatcagcc gatattcgtc aaccgacccg gcgattgatt
     9361 tttttattaa ggaaaacaat ggatctgtta atcgcttttc ttgtgctgat tatttccgtt
     9421 ttattcagtc tgtttcaaaa cttttcctta ctcatcccgt tgggaatcgg tttgatctgc
     9481 tttatttcgg tcggtcttta tcgccactat ccgcttcgtt ctttatttaa aatggctttg
     9541 gtgggtgcaa aaaactccct tgtcgtcatt cgggttttgg ttttaatcgg ttgcctgaca
     9601 gccttgtggc gagcttgtgg aacgatttct ttctttgtgg ttttcggcgt gaagctcatt
     9661 acgccccata ttttcatttt ggtggccttc ttgctttctt cccttgtttc ttatgccatg
     9721 ggaaccagct tcggcgtagc cggcacggtg ggagtgattt taatgtcaat cgcccgagct
     9781 gggggtgtca acgaatatat tgccgccgga gcgattttat ccggtgtgta tttcggagac
     9841 cgaacggcgc cgacatcctc ttgcgccaat ttggttgctt cgcttacgaa gaccgatctg
     9901 tacgataatg tgaagcgcat gctgaaatcc gccgcgcttc ccttcgtgat ttgtatcttt
     9961 ctttacgccg gtttatcttg gaagaatccg ctttcaagcg cggatacatc cttggtcagc
    10021 gttttggaaa acaatttttc gctctcggtt tggaccctta tcccggctgt tatcttatta
    10081 attttgccat tgtttcgcat tccggtaaga atcgccattt caatcagtat tttattttcc
    10141 ttcgcggtca gtatgggaag tcagcaaatg gggtttgagg atacttttca tgcgatgctg
    10201 ttcggttaca agccctcaga ccccactttg accggtattt tatccggagg cggattgatt
    10261 ccgatgctta ccaccattct tatcgttgcg ttatcgagca catattctgg catttttaat
    10321 gggacgaata tgatttcagg aattcgggag aaactattcg atctctccga aaaaatcggc
    10381 cgtttcccca cggtggtagc gacgagcatt ctcagctgcg cagttttttg taatcaaacg
    10441 atgggcatta tattcacaca tgaaatggtc ggaaaaatct atcaagagaa aaaaattgat
    10501 cgaagcactt ttatgttgga tatcgccaat tcaaccacgc tgattgccgt actgattcct
    10561 tggtcaatcg cttcttcggt tccgttgact tggatgggtg tgaatttcaa agcgttacct
    10621 tatgcttttc tgctttattt aatccctttg ttctatggaa tacaggaaca acataaatca
    10681 aaacaacatt aaacacttta aaaaaaatat tctttacatt cttttgtcga taaatccagg
    10741 tatttttttg attacaatta aacagaactc cctttataaa aaaggagttg tcaaaaagga
    10801 ggtctgttat gccttggaca acaaaaaatt atccgaatag ttggaaaaac tttgatccgc
    10861 tgttgcggaa aaaagcgatc gatatcgcaa atgccatgtt ggctgacggc tataaagaag
    10921 aggacatcat ccccatcgcg accagccagg cgcaaaaatg gtatgaaaat gccactcccg
    10981 ctgaactgaa agaattagaa gagaaagatg tcaccaaaca tgccaaaccg aagggtcctt
    11041 ccggtgcgcg cctcatggaa aaagacgtaa ttgtccgata tcaacccgag caaaaacaat
    11101 gggaagtcat ttccgatggc gcactgcagg cggatagtct gcatcatacc aaaaaggaag
    11161 ccgtcatccg tgcccgagaa atcgccggaa agcgtggcac tcgtgtgatt gagaaaaaga
    11221 aatccgaaag ctgaaagcag ccggaaaata aattcttttt caccgtagga accggcaggc
    11281 tgtcgattga cagcctgcct atttaaatgt ttttgtcgga gaagaaaatg atttattctt
    11341 tttatgaata aatcattttt ttagaatgat tctcaccggt aaaatcgatc cgttattgaa
    11401 agttatttga agtggtataa ttcgcccgcg aatggaatat ttaagatttt attttatatc
    11461 gcctcttcgg aaatcaggta attttattcc ccaatttgat atttgctcaa tcgataaaga
    11521 ttaataaaaa aggaaaatat ggcactggct gttttcttat ttgtacttac atacattctt
    11581 cttttggcgc ttccgaaata tcgagcttat gtggcattgg tttccgccat tcttttcatc
    11641 ggcactggca tcatctctcc ggtgaaagcc gtcgaaggca ttgattggaa tgtgctcatg
    11701 atgattaccg gtacgatggg tacggtttat cttttttgcc aatcccgaat gcccgaaaaa
    11761 atggcggatg ttattttaga gagaatgccg gatgtcaaat gggtcattgt cagtttggct
    11821 ttgttcgccg gaattatctc cgctttcgtg gataatgttg ccaccgtttt gatggttgct
    11881 ccgatcgcat tgaccgttgc aaaacgattg aaaatttcac cggttccgat catcatcgca
    11941 attgccgttt cttcaaacct tcaaggggcg gcgactctcg tcggggatac cacggcgatt
    12001 atgatgggca gtcaagccgg tatggatttt attgatttct tcttcttcca tggtcgaccc
    12061 agcatgtttt ggttcaccga agtcggagca gttgtttccg cactcatctt gctttggcaa
    12121 tttcgggata aaaacgaaaa agtcaccgat gtgttgaata ccgaagtgaa tgattatgtg
    12181 ccttcgattt tgttatttgg gacggtgtta ttgttgattg tcgcctcatt tataaatccc
    12241 aagccgaaaa taaccaacgg cttgatttgt atgagcatta tgttgatcgg cattgtattc
    12301 gaaggaatta aaaatcatgg attagccgat tctttccgag aaactttcgg atcgattgat
    12361 tttttcacat tactactttt ggcaagcctc tttatggtga ttgcgggtat taaggaagcc
    12421 ggcgtaatcg acagtttggc ggcacttttt gtcaggttat ccggaaacag cctgattctt
    12481 atctattctc tgattttatg gggctcggtg ttgatatccg cgttcatcga taacatccct
    12541 tatgtcgcaa caatgctccc tgtcgtagcc gggattgctt cttatatgca aatagatccc
    12601 actcttctct atttcggact tctttccggc gccacgctgg gcggtaattt aacacccatc
    12661 ggcgcatctg cgaatataac cggaatcggg attctgcgaa aaaacggcta tgaaccgtcc
    12721 acccaagatt ttatgaaaat cagtgttcct tttactttat cggcagtcac
//
LOCUS       DAOSMM010000062        12594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_4990_length_12594_cov_15.500518, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000062 DAOSMM010000000
VERSION     DAOSMM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4990_length_12594_cov_15.500518"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /locus_tag="PK898_08385"
     CDS             complement(<1..1995)
                     /locus_tag="PK898_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOU20087.1"
                     /translation="MSFESFFYPHAVVVNGSMSEGKLGQVLLKKIIDGGYEGKLFAVN
                     PKAQGAFGIQGFPSVASIGEPVDLAVIVSPASTVPSVLDDCGKAGVKAAVVITAGFSE
                     MGNKAGEDEVLEAAKRNHIRVIGPNCAGIVCTSHKFFPTLEIHPPQGGVALVSQSGAL
                     GGVVLAWAQEQGLGISKFVSYGNGIDVNQVELLRYFKDDPETKVVAVYIESIRNGREF
                     MEALEACSKIKPVVVIKAGRTNVGQRATASHTGSMAGADAVYDAAFRQCGAVRVKTVE
                     EMMDLCTGFVSLPPLNGNRVIIVTNSGGPAVLAADMGEEVGLNMAEVNPKTVETLKSF
                     LPPHCGFRNPIDLTVEGTEDGYRKTLLTVLDEFDAAVAMNICPPYLDSMKLAKGVADA
                     AKISGKPIVANFLPNQVVKESVAYLKDQGIPNYVSGERAVQSLAKMEQYSSMIRDIRE
                     DMGFTETWESLKKRIGSETYKLPGERQILEPEAMAWLRDNQIPTPKFGVAFSPDEAAK
                     VCEDVGFPCVMKVVSPQILHKSEMGGVIIGIRDAASAKQAFETIKRRAEGKDFQGVII
                     YPQISGAQEVLLGLVEDSQFGPVVAFGLGGIYTEIFHDIVLNIAPVNTAQATRMIQSI
                     KGIKILQGARGAKPYDLDALARTISRFSELPFIYPEISEAD"
     gene            complement(1992..2615)
                     /locus_tag="PK898_08390"
     CDS             complement(1992..2615)
                     /locus_tag="PK898_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU20088.1"
                     /translation="MKYDVFMVGVGGQGILSIGEIIASAALTKGIPVNFYPSKGMAQR
                     GGFVKASLRLGGETVGPNIAEKGADLVLSMEVSESLKAIRYVKPGGDFVLLGNIWAPT
                     AVMLGKAGYPSVQDVIDQIKQAGANLVYINEADVPSYDGIKVADNIFMLGIIMKYTKL
                     SQIISVEDIRKVIEERWPKAVEINRYALQCGLDYIPEQIPSVSEGTK"
     gene            complement(2612..4489)
                     /locus_tag="PK898_08395"
     CDS             complement(2612..4489)
                     /locus_tag="PK898_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU20089.1"
                     /translation="MKKLITGNDAIAYGALAAGVKVAVGYPGTPSTGAIAALLDMDLP
                     QTHVEWSTNEKVAIDIAGGVAWAGHRVLCTMKMSGLNVAYDSLISLAYSGCDGGFVIY
                     VADDPGVSAGMCEQDSRGFALMSDMPMLEPTTVEETMHLTKYAFDLSEQIHGPVFIRL
                     TTSLSNAFADAEIIEPAPVSQAEPLLIRDIDRFTKAGAAVCMKQHRDLINRLAKAAEI
                     IDIDRLNSLNLSKKSGGLGIVTGGITIRYLDEALDIAKEYGLDPDSVSILSVKASVPF
                     PKNQAKQLLNHCDSILVLEELEPYIEKELYILARQVGYKGTIVGKLDGTLERIGEYGL
                     SQALIGISKATNIDIPSSVYQSDTKAESIAAARPITVCSGCPHRGTYMAINSAIRKLH
                     LKQKDVMVAGDIGCTILGMNPPFNTVWNEVSMGTSVSLAQGYVYAGVKTPVIATIGDS
                     TFFHGGIPGLINAIQHQVNMTLIIMDNRWTAMTGMQINPGTPPELHHKDYKEIDIAKI
                     VPALGVDQFFEINPFDQDDAVETIVKALSLPGVKVILSRQECAIQAARHKDKNAPQKI
                     VRVIDENCNLCKLCIAVTGCSALVPGKDSMTIDPSLCQQCGLCVGVCNRNALVMEEVS
                     K"
     gene            complement(4550..5665)
                     /gene="hisC"
                     /locus_tag="PK898_08400"
     CDS             complement(4550..5665)
                     /gene="hisC"
                     /locus_tag="PK898_08400"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU20090.1"
                     /translation="MFDVNKIARPCILVRKEYVPGKPVEEVTREYGITDIIKLASNEN
                     PLGASPLAVEAMIKEIRANANYYPESLCPKVAEKLAEKFNLSPNNFYIDNGEDGVITM
                     LGLTFINPGDECIMAELTFPAYENIVGKMDGKSIVIPMTKDYRFDVDGMIRAITPKTK
                     IIFLCNPNNPTGTIIYKDEFERLLAAVPENTLFVCDEAYYEFVDDPNYPQSLDYFNDH
                     PNLIVMRTFSKIMGLASVRIGYAIAHKEIVQVMLKTREPFPVNRVAQAGALAAMDDKE
                     FCDKTFTVNAAGRKQLFEGLKQMGFEVYAGQANFIFANLGKPSMPVFEGLLKEGVIIR
                     PLAPQGAPNCIRVTIGTPEQNERFLNSFRKVFSEVYP"
     gene            6205..7119
                     /locus_tag="PK898_08405"
     CDS             6205..7119
                     /locus_tag="PK898_08405"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HOU20091.1"
                     /translation="MDFKELESFVEIAKVKSFSTAAKNLFLTQPALSNHIAKLEKELG
                     IALFERNNKKTELTPAGKQFYLSALEILNQRENALLNLEKFQGKIDGMLHIATSSVPG
                     QYILPNILKNFNTKYPDVTYDLHYLSSGEITDMLMMGELDLGFIGAEPDNRKIVYEKI
                     ADDELVVIAPNISPFNKMNTISFERLLDEPLLLRKEGSGTRQAFDLAYKKHAYLGKPA
                     KVLSYMDNNEMIVLCVKAKLGLSIVSRISVEDKVKAGFIKVLPLEEYNFQHAFYFVYP
                     KDRTMSPLVNRFKDFVLAERNSKEIFEE"
     gene            7529..8305
                     /locus_tag="PK898_08410"
     CDS             7529..8305
                     /locus_tag="PK898_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20092.1"
                     /translation="MALLETKSISIQFGGLWAVKNVDFQIDSGQTFGLIGPNGSGKTT
                     FLNIISGIYSATNGRVFLEGEDITDLKPYQIFKKGISRTYQSSRLCWEMSVADNLLVG
                     TYTSQDYGLADTIFHPKKVNNRLAELVENSLDLLSYFNPGLIEKRYELAKNIPHIDRR
                     RIEITRALLSKPKVLLLDEPTAGLNDDETMTMMDDIQKIKDKVGNIAIIIIEHDMKVM
                     EKVPQRVIVFNAGEKIAEGTFQEIINDPNVIKAYIGGEAK"
     gene            8305..9018
                     /locus_tag="PK898_08415"
     CDS             8305..9018
                     /locus_tag="PK898_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20093.1"
                     /translation="MGEVILEIKNLFTSYGRIPMLMDISLTVHQQNICCLLGPNGAGK
                     TTLINAIIGTVNADKGQIIFKGEDITNLPTHDRIAKGIQVASAGVGTFPKMTVETNLR
                     LGAYNINDEKLLAQRLEEIYESFPILKGRLEQKAGTLSGGERTMLSIARAVINKPDLL
                     IMDEPSLGLAPIVVDEVFHIVKRLNQENGMTIMLVEQNIAKALSVSKFGYIIQKGQII
                     YSGPSEQLVDCDALKNPVM"
     gene            9057..10241
                     /locus_tag="PK898_08420"
     CDS             9057..10241
                     /locus_tag="PK898_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU20094.1"
                     /translation="MKKSYRFIIVLLLFVVVLIGAFQKTAFAAKDVVTLGFLGPITGS
                     CAAEGSAARNAFLMAIDDANASGEYPYKINVIVVDDQSDPSVGAAGAQKIVADPTVVA
                     ATGHWNSGVAAATSPIFIENEIPLLIWGAIREDLTSKETYPWITRSAPTNVQENKPLV
                     KAVIDDMGYKNIFMVSTTDSYGSANTEAFSKELESRGMKPVGLEEVQAETVDFNAIVA
                     KIIKSGADAVYWGGTNTEGVYLKEQLYNAGADVLFCGISGIKTDDFLIIGEKAAEGTL
                     AVQPGIRLDSTEAGRKFVEEYNSKGFDVEINAFTPYAYEAALILLQALKNCGDEPTPE
                     KMRDAIAKGTFVGIMGTTTFDEIGQTTLVAANLMVIQDGKWMYYEDSEYASGKRSFGG
                     KK"
     gene            10369..11334
                     /locus_tag="PK898_08425"
     CDS             10369..11334
                     /locus_tag="PK898_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU20095.1"
                     /translation="MEIFSSLFVSAILLGCTYTLVALGYCLFFGVLDVVVFCSGDIAI
                     FGAFSVISAYILTYDAGIFQSLPLPVGTLIVLAIAAVLCVGLSLATYFVSVKPFSKSS
                     ALMPLLSTIALGTAIKELLGLAYKPIMSNLIRAVTRIDTPINVTSGRNPQAMPKLLNA
                     GGSTTERDFIIMGLTLLILFVLFLFLNKTKMGLSMQAISQNKELAYMSGINVKKTINT
                     TFIIGGIVLTIAGFLLGSYQSVIRFDTGSLYGTKGFSAAVVGGLKSIWGAVIGGMLLG
                     FVEVFVSGLIPGGTQYQNIIAFIVVIIFIVFKPEGILGEKTIEKV"
     gene            11355..12572
                     /locus_tag="PK898_08430"
     CDS             11355..12572
                     /locus_tag="PK898_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU20096.1"
                     /translation="METLKTKIDSPAAVLLTNIVLAVLIFFILMSDWILSIGLMILFI
                     AGALILDQNGIINLIFDAYNHYKHIAILSGIVLVIMLPFSLTGNNYISHIATVAVCYA
                     IACLGLNFQMGSTDMTNFAPAAFMGLGAYSVAVCTTKFGLSPWTGMILGILITGVFGY
                     LIGLPTLKTKGYYLSLVTMAIQIAFTQLINTIDYLGGSDGISGVTKYSIFGFKLYKKY
                     TVFGVKLAPQIPFLVVCIFFLIICAYIAIRVYHSKQGLALNAIAQDEIAANCLGINIA
                     NQKLFAFVIGSIFCGLAGSLLVGLEGYVGPDSYDFSKSLMLICMVILGGMDNSIGIIV
                     GAFILTIISEKLRDFADYQQLVYSLVLVIMLIIRPNGIIPRRVRKYFNAFRSSPVAKT
                     IEAEKAQAIDFNK"
BASE COUNT         3681 a         2521 c         2578 g         3814 t
ORIGIN      
        1 atcagcttcc gaaatttccg gataaatgaa cggcaattcg gaaaaacgag aaatggtccg
       61 ggcaagtgca tccaagtcat atggctttgc tcctctggcg ccctgtaaaa tcttaattcc
      121 cttgatcgat tgaatcatac gagtcgcttg agcggtattt acgggagcga tattcaaaac
      181 aatatcatgg aaaatctcgg tataaattcc tcccaaacca aatgccacaa cggggccgaa
      241 ttgggaatct tccaccaatc ccagcaatac ttcctgagca ccagaaattt gcggatagat
      301 aataacacct tgaaaatctt ttccttcggc gcgtcttttt attgtttcaa aggcttgttt
      361 tgccgatgct gcgtcacgaa tgccgatgat cacgccgccc atttccgatt tgtgcaatat
      421 ttgcggtgaa acaactttca tgacacatgg gaaaccgacg tcttcacata ctttcgcagc
      481 ctcatcagga gaaaaggcga caccgaattt tggcgtggga atttggttat ctctcaacca
      541 agccattgct tccggttcaa ggatttgccg ctctcccggc agtttatagg tttcggatcc
      601 gattcgtttt ttcaaagact cccatgtttc ggtaaaaccc atatcttccc gaatgtcgcg
      661 gatcattgaa gaatattgtt ccatttttgc gagcgattga actgctcgtt ctccgctgac
      721 ataattaggg ataccttgat cttttaaata agcaacgctc tctttaacta cctgattcgg
      781 taaaaaattt gccacaatcg gtttcccgct gatttttgcc gcatcggcca caccttttgc
      841 cagtttcatt gaatccaaat aaggcggaca gatattcatt gcgacagcgg catcaaactc
      901 atccagaacc gttaacaatg ttttccgata tccatcttcg gtcccttcaa ccgttaagtc
      961 gatcggattt cgaaaaccgc aatgcggcgg aaggaatgat ttgagcgttt cgacggtttt
     1021 cggattgact tcggccatat tcagtccgac ttcttcgccc atatcggctg ctaaaacagc
     1081 cggtccgccg gagtttgtga cgatgatgac gcgattccca ttcagcggcg gcaaactcac
     1141 aaatccggtg catagatcca tcatttcttc aaccgtcttc acccgaacag ctccgcattg
     1201 tctgaacgca gcatcgtaaa cggcgtcggc acccgccatc gatcccgtat gggaggctgt
     1261 cgctcgttgt ccgacatttg tccgaccggc ttttataacg acgaccggtt tgatcttgct
     1321 gcaggcttcg agcgcttcca tgaattcgcg tccatttcga atgctttcaa tatatacagc
     1381 aaccaccttc gtttccggat cgtctttgaa atatcggagc agctctactt gattaacgtc
     1441 gattccgttc ccgtaactga cgaatttcga aatacccagc ccctgttctt gtgcccaggc
     1501 taacacgacg ccgcccaaag ctccgctttg cgaaaccaac gcaaccccgc cttgaggcgg
     1561 atgaatttca agtgtcggga aaaatttgtg gctggtacag acaataccgg cgcagttcgg
     1621 gccgataacc cggatatggt tccgtttagc cgcctcaaga acctcatctt ctccggcttt
     1681 attccccatt tcagagaatc cggcagtgat cacaacagca gcttttaccc ctgcttttcc
     1741 gcaatcatcc aagacggacg ggaccgtgga agcgggtgaa acgataacag ccagatcaac
     1801 cggttctccg atagatgcca ccgacggaaa tccttggata ccgaaagcgc cttgtgcttt
     1861 cggatttacc gcaaacaatt tcccttcata tcctccgtca attattttct taagcaaaac
     1921 ttgtcccaat ttaccttccg acattgagcc gtttacgaca accgcatgag gataaaaaaa
     1981 ggattcgaaa ctcatttggt accctcggaa acagacggaa tttgttccgg aatgtaatcc
     2041 aaaccacatt gcaacgcata acggttaatt tcgactgctt taggccatct ctcttcaatc
     2101 acttttctga tatcttccac cgaaataatt tgtgaaagct ttgtatattt cataataatt
     2161 cccagcatga aaatattatc agccactttg atcccatcat aagaaggaac atctgcttca
     2221 ttgatataga cgagatttgc tccggcttgc ttgatttgat cgattacatc ctgaacagaa
     2281 ggataccccg cttttccgag catgactgct gtcggtgccc agatattacc gagcaagaca
     2341 aaatctccgc cgggtttaac ataacgaatc gcttttaaag attccgatac ttccattgat
     2401 aaaacgagat cggcaccttt ttcagctata ttcggtccta cggtttcccc tcctaagcgc
     2461 agactggctt ttacaaaacc accccgctgt gccatcccct tactggggta aaaatttacc
     2521 ggtattcctt ttgtcaaagc tgcgcttgcg ataatctctc cgattgatag aatgccttgt
     2581 ccaccgacgc cgaccataaa aacatcatat ttcattttga aacctcctcc ataaccaaag
     2641 cgttccgatt gcacactccg acacaaagcc cgcattgctg gcaaagagac ggatcaattg
     2701 tcatagaatc tttccccgga actaacgcgg aacaaccggt tactgcgata cataatttac
     2761 aaagattaca gttctcatcg attacgcgga caattttttg aggagcgttt ttatctttat
     2821 gacgggcagc ctggatagca cattcctgtc tcgaaaggat tacttttact cccggcaacg
     2881 acagtgcttt cactatcgtt tcaaccgcat cgtcctgatc aaacggattt atttcgaaga
     2941 attgatctac acctaaagcc gggacgattt ttgcaatatc tatttcttta taatccttat
     3001 gatgaagttc gggcggtgtt ccgggattga tttgcattcc cgtcattgct gtccaacggt
     3061 tatccatgat aattaaagtc atatttactt ggtgttggat cgcattgatc aaaccgggga
     3121 taccgccgtg gaagaaggtg gaatcgccga ttgtcgcaat tacgggagtt tttactccgg
     3181 cataaacata gccttgtgcc aaactgacac tcgtacccat ggatacttca ttccaaacgg
     3241 tattaaaagg aggattcatt cccagaattg tgcacccaat atcgcctgct accataacat
     3301 ctttttgttt cagatgtaat ttacgaatcg cactgttaat ggccatatat gtcccgcgat
     3361 gcgggcaacc tgaacagacg gtaatcggtc tggcagccgc tatcgattcg gctttggtat
     3421 cactctgata gacagaagag ggaatatcga tatttgtggc tttcgaaatg ccgatcaacg
     3481 cctggctcaa tccgtactca ccgattctct cgagtgttcc gtccaatttt ccgacaattg
     3541 tccccttata tccgacctga cgggccagaa tgtatagttc tttttcgata taaggctcta
     3601 attcttccaa gactaaaatc gaatcgcaat gattcagcaa ttgctttgcc tgatttttgg
     3661 gaaacggaac agatgctttg accgataaaa tcgataccga atcgggatcc aatccatact
     3721 cttttgcgat atctaatgct tcatcaagat atcttattgt gattccgccg gtaacaatcc
     3781 ccaaaccgcc tgattttttt gacagattca aggaattcaa ccggtcaata tcgataattt
     3841 cagctgcttt ggctaatcga ttgatcaaat cgcggtgttg tttcatgcat accgctgctc
     3901 cggctttggt aaagcggtcg atatcccgga taaggagcgg ttccgcttgt gagaccggtg
     3961 caggttcgat gatttccgca tcggcaaatg catttgacaa cgaagtggta aggcggataa
     4021 ataccggccc atgaatttgt tccgaaaggt caaaagcata ttttgtcaga tgcatggttt
     4081 cttcgacggt ggtcggttcc agcatcggca tatctgacat taatgcgaaa ccgcgtgaat
     4141 cttgttcaca catccccgcg ctgacgccgg gatcatcggc cacataaatc acaaatccac
     4201 cgtcacatcc ggaatatgca agcgaaatta aagagtcata agcaacattt aatccggaca
     4261 ttttcatcgt acataaaaca cgatgacctg cccaagcgac ccctccggcg atatcgatgg
     4321 cgactttttc atttgtgctc cattcaacat gggtctgagg cagatccata tccaaaagag
     4381 ctgcaatcgc tccggttgac ggtgtgccag gatatcctac cgcgactttg actcccgcgg
     4441 ctaaggcacc gtaggcaatc gcatcatttc cagtaattag tttcttcaat ccgaacctcc
     4501 ctgttcattt ctaaaataga tgcattaatg ataatttcat ggctttaaat tacggataca
     4561 cttccgaaaa aacttttctg aaggaattca gaaatctctc gttctgttcc ggagttccga
     4621 tcgtaacacg gatgcagttc ggtgctcctt gcggggcaag cggtcggata attactccct
     4681 ctttgaggag cccttcaaaa accggcatgg acggtttccc taaattggcg aaaatgaagt
     4741 ttgcctgacc ggcatatacc tcaaaaccca tttgctttaa gccttcaaaa agctgttttc
     4801 ttccggcggc atttactgta aatgttttat cacaaaactc tttatcatcc atggccgcaa
     4861 gtgctccggc ttgggcaacg cggttgacag ggaacggttc tctggttttc aacataacct
     4921 gaacaatctc cttatgggcg attgcataac cgatacgaac ggaagcgagc cccataatct
     4981 tggaaaaagt acgcatcacg attaaattag gatgatcgtt aaaataatcg agtgattggg
     5041 gataattcgg gtcatcgaca aattcatagt aagcttcatc gcaaacaaat aaagtattct
     5101 ccggcacagc ggccaataaa cgctcgaatt cgtccttata gataatcgtt ccggtcgggt
     5161 tattcggatt acataggaaa atgattttcg ttttgggtgt aatagcccga atcatcccat
     5221 cgacatcaaa gcgataatcc tttgtcatcg gaatgacaat acttttcccg tccatttttc
     5281 cgacgatatt ttcgtaagcc gggaaagtca gctcggccat gatgcattcg tctccgggat
     5341 taataaaagt gagtccgagc atagtaatta caccgtcttc tccattatcg atataaaaat
     5401 tattcggaga aagattgaat ttttcagcta acttttctgc cactttcgga cataaacttt
     5461 ccggataata atttgcgttt gcgcgaattt ctttgatcat tgcctcaacc gcaagcggtg
     5521 aagcgcctaa gggattttca ttcgatgcta atttaataat atcggtaatc ccatattcac
     5581 gtgtaacttc ttcaaccggt ttaccgggta catattcttt acgtacaaga atacaaggac
     5641 gagctatttt attgacatca aacacggctt tacctcctga aatataattt cctttattaa
     5701 tcattttgag aaatcggata atctcctgtt tcccataaag ttttataaaa atatttcaaa
     5761 aaaatcgatc gttatcgtaa aatgttctgc tataagaaat attcttatcc gcaagttcaa
     5821 gctaagaaaa caatatttcc ttccttcagt ataacattct tagatattat gaaacaatga
     5881 ggataattag ttttttctta ttacatttat acatgctatc atgaattttg acaatggtaa
     5941 aatattgttt acaacttctt caattatggc tatattattc aaaaagataa gttttttaag
     6001 atagtatatt agtgatttag caagataaat tgatagatga tctaattact ataaaatatc
     6061 ggaatattgt aatttatata aaatgttttt ggtaaacagt taagaaaaac aaaaataatt
     6121 tgaatccgct ataattataa gaacataaaa ttcatacgtg tttttatcat acattgtttt
     6181 atttatttga agtgaggtgt caatatggat tttaaagagt tggaatcatt tgtggagatt
     6241 gcaaaggtta aaagtttttc tactgcggca aaaaatttat ttctgacaca gcctgcatta
     6301 agtaatcata ttgccaaact ggaaaaagaa ttggggatcg cattatttga acgaaataat
     6361 aaaaaaacgg aattgacacc tgcggggaaa cagttttatc tatcggcact cgagatttta
     6421 aaccaacgtg aaaatgcatt gcttaatttg gaaaagttcc aaggaaaaat cgatggcatg
     6481 ctgcatatcg caacgagcag tgtccccggt caatatatcc ttccgaatat tttgaaaaat
     6541 ttcaatacaa aatatccgga tgtaacttat gatttgcatt atctcagttc gggtgaaata
     6601 accgatatgc tgatgatggg tgaattggat ttgggattta tcggtgccga accggataat
     6661 cgaaagattg tctatgaaaa aattgcggat gatgaattgg tagttattgc gccgaatatc
     6721 agtccgttta ataagatgaa taccatcagc tttgagcgat tgttggatga gccgttgctg
     6781 ctgcgaaaag agggatccgg aacgcggcag gcttttgatt tggcttataa gaaacatgct
     6841 tacttaggca aacctgccaa agtattgtcc tatatggaca ataatgagat gatcgtatta
     6901 tgcgtcaaag caaaattagg tctttcgatt gtatccagga tttcagtcga agacaaagta
     6961 aaagccggat ttatcaaggt ccttcctttg gaagaatata attttcaaca tgctttttat
     7021 ttcgtgtatc cgaaagatcg aaccatgtct ccgttagtca atcgatttaa ggatttcgtt
     7081 ttggcggaac gaaattcgaa agaaatattt gaggaataaa atgatatttg cttccgatgt
     7141 gatagtcttg ataaatcgta ttagagaaaa tcataggagt agctaatagt taattataaa
     7201 gatctatata taagaggtaa catatactta ataagaaaat attaattaaa tgaatattta
     7261 ataatttaga ttgaaaaatt cacagaattt tattcataac tttttgttca ttagattatt
     7321 ttatttgata gccggataac ctttcccgtg cataaataaa tgatattatt tgaaatcagt
     7381 cattgaaaag atttatttga gtaaatcact aaattcttaa atacaagatt gaaatattaa
     7441 aacgggacca aaataaaaat caaggttcca tattatttca gaattttata ttgggcaatt
     7501 ttgatattcg gagaggaatt ttacttatat ggctctttta gaaactaaat ccatttcaat
     7561 ccaattcggc ggactctggg ctgtaaaaaa cgtagatttt caaatcgact caggacagac
     7621 atttggattg atcggcccta acggttcagg gaagactacg tttctaaata ttatcagcgg
     7681 gatttattcg gcgacaaatg gaagagtatt tcttgaagga gaagatataa cggatttaaa
     7741 accttatcaa atctttaaaa aggggatttc aagaacgtat caatccagcc gactttgttg
     7801 ggagatgagc gttgccgata atttactggt agggacttat acatctcagg attacggttt
     7861 agcggacaca atatttcatc ctaaaaaagt caataaccgc ttggctgagc tcgtagaaaa
     7921 cagtcttgat ttactgagtt atttcaatcc cggtttaatt gagaaacgtt atgagcttgc
     7981 gaaaaatatt cctcatatcg atcgaagaag aatcgaaatt accagggcat tattaagcaa
     8041 acccaaagtg ttactgctgg atgagccgac agccggtctg aatgatgatg aaacgatgac
     8101 tatgatggat gatattcaaa agataaaaga taaagtcgga aatattgcca tcattattat
     8161 tgaacatgat atgaaagtaa tggaaaaggt tccgcaacgt gtcattgtat ttaatgcggg
     8221 agaaaaaatc gccgaaggta catttcaaga gattattaat gatccgaatg ttatcaaagc
     8281 atacattgga ggcgaagcaa aataatgggt gaagtaattc ttgagataaa aaatttattt
     8341 acttcgtatg gtcgtattcc aatgttgatg gatatttctc taacggtgca tcaacaaaac
     8401 atttgctgtt tattaggacc gaacggtgcc gggaaaacaa ccttaataaa tgccataatc
     8461 ggtaccgtaa atgcggacaa gggacaaatt atattcaaag gggaagatat tacaaacctc
     8521 ccgactcatg acaggattgc aaagggcatt caggtcgcat ccgccggtgt cggcacattt
     8581 cccaaaatga cagtagaaac taacttgcga ttaggcgctt ataacattaa cgatgaaaaa
     8641 ttattagcac aacgactcga agagatctat gaatcatttc cgatactgaa agggaggttg
     8701 gaacagaaag cagggacgct atccggagga gaacggacta tgctttctat tgcgagagca
     8761 gtgattaata agcctgatct gttaattatg gatgaacctt ccttaggtct tgcaccaatt
     8821 gttgttgatg aggtttttca tattgtgaag cgattaaatc aagaaaatgg tatgacaatt
     8881 atgttggtcg agcaaaatat tgcaaaagcg ttatccgttt ctaaatttgg ttatattatt
     8941 caaaaaggac aaattatcta ttcgggtcca agcgaacagt tagttgattg tgatgcgttg
     9001 aagaatcccg tcatgtgaaa atatgatggg tttaattatt tagtggagga aaaagtatga
     9061 aaaaaagtta tcgattcatt attgtgttgt tgctttttgt tgttgtccta atcggagctt
     9121 ttcagaagac cgcatttgcg gctaaagatg ttgtcacact tggattcctt ggtccgatta
     9181 ccggttcatg tgcagcagaa ggatcagctg cgcggaatgc atttttgatg gctatcgatg
     9241 atgcaaatgc aagcggcgaa tatccctata aaataaatgt gatcgttgtc gatgatcaat
     9301 ccgacccttc tgtcggagct gccggcgcac aaaagattgt tgctgatccg acagtagttg
     9361 ccgcaaccgg ccattggaat tccggtgttg cagcggcgac cagtccgatt tttatcgaaa
     9421 acgaaattcc gctattaatt tggggagcga ttcgcgaaga tctgacctca aaagaaacct
     9481 atccgtggat aacccgttcg gcaccgacga atgttcagga aaataagccg ttggtaaaag
     9541 ctgttatcga tgatatgggc tataaaaata ttttcatggt ttcaaccacg gattcctatg
     9601 gatctgccaa tacggaagct ttttcaaaag aattggaatc cagagggatg aaaccggttg
     9661 gccttgagga agtccaagcc gagacggttg acttcaatgc tatagtcgcc aaaattatca
     9721 aaagcggtgc ggatgccgtt tattggggcg gtaccaatac ggaaggcgtt tatctcaaag
     9781 aacaacttta taatgccggt gccgacgttc ttttctgcgg aatttcaggc attaagacag
     9841 atgacttttt gatcatcggt gaaaaagcag cagaagggac gcttgccgtt cagccgggta
     9901 ttcggcttga cagtaccgag gctggaagaa aatttgtgga agaatacaat tccaaaggat
     9961 tcgatgtcga gatcaatgca tttactccgt atgcttatga agcggcactt attttgttgc
    10021 aggctctgaa aaattgtgga gatgaaccga cacccgaaaa aatgcgagat gccattgcaa
    10081 aaggaacatt tgtcggaatt atggggacga caacgttcga tgaaatcgga caaaccactt
    10141 tggttgcggc aaacctcatg gtcattcaag acggaaaatg gatgtattat gaagattctg
    10201 agtatgcaag cgggaaacga tcttttgggg gaaagaagta aattttaatt cgtagtaata
    10261 aggaagaggc ttaggggatg attcctaagt ctcttccccg aataaacctt ttcttttatt
    10321 tcccatttgt tcaatcatcg aacaatagaa aggtatcgtc ttaatcatat ggaaattttc
    10381 tcaagtttat ttgtcagcgc aatccttctt ggttgtacat atacactggt agctttgggg
    10441 tattgtcttt tctttggtgt tctggacgtt gttgttttct gtagcggtga tattgcaata
    10501 tttggagcat tttcggtaat cagtgcatat attttgactt atgatgccgg aattttccaa
    10561 tcattgccgc tgccggtcgg aacattaatt gtactggcaa tcgcagcagt tctttgcgtc
    10621 ggactttctt tggctactta ttttgtctct gtaaaacctt tttccaaatc ctcagcttta
    10681 atgccattgc tttcaacaat tgctttggga acagcgatta aagaattatt aggtctcgct
    10741 tacaagccga ttatgtcgaa tcttatccga gcagtaacaa gaatagacac tccgattaat
    10801 gtaacatccg gaagaaatcc acaggctatg ccaaagcttc tcaatgcggg aggttcgacg
    10861 acagagcgtg acttcattat tatgggactt acactgctta ttttatttgt tctcttttta
    10921 tttttaaata aaacaaaaat gggattatcc atgcaagcaa tttctcaaaa taaagaactt
    10981 gcatacatga gtggaattaa tgtaaaaaag acaatcaata caacttttat aatcggcggt
    11041 attgttctta ccattgccgg ttttttactt ggaagttatc aatcggttat ccgatttgat
    11101 acaggatcgt tgtatggaac caaagggttc tctgcagcgg ttgtcggcgg attaaagagt
    11161 atttggggtg ctgtaatcgg cggaatgtta ttaggttttg tagaagtttt tgtttccggt
    11221 ttgatccccg ggggaactca atatcaaaat attattgcat ttatcgtagt catcatcttt
    11281 atcgttttta agcctgaagg aatattaggc gaaaaaacga ttgaaaaagt ttaggaggac
    11341 agaaatcaac cgatatggaa acattaaaga cgaaaattga ctctccggca gctgttctct
    11401 taacaaatat tgtattagcc gttttaatat tttttatttt gatgtccgat tggattctca
    11461 gcatcggttt aatgattctt tttattgccg gggcattgat tcttgatcaa aacggtatca
    11521 ttaatctcat attcgacgca tataaccatt ataaacatat tgcgatttta tccggaattg
    11581 tattggttat tatgcttcct ttctctctta ccggaaataa ttatatttct catatagcta
    11641 ctgttgcggt ttgctatgct attgcttgtt tggggcttaa ttttcaaatg ggttcaacgg
    11701 atatgacgaa ttttgctccg gcagctttta tgggtttagg tgcctattcg gtagctgtct
    11761 gtacgacaaa attcggatta tctccatgga cgggaatgat attggggatc ttaattacag
    11821 gagtttttgg ttaccttatc ggattaccga cattaaaaac aaaaggatat tacctttcct
    11881 tggtcacaat ggcaatacaa attgcattta ctcaattgat caataccatt gattatttgg
    11941 gaggttctga tggaatttcc ggagtaacta aatactcgat atttggtttt aaattatata
    12001 aaaaatatac tgtgttcgga gttaaactcg caccacaaat cccctttttg gtagtttgta
    12061 tttttttcct gataatttgt gcttatattg caattcgtgt ctatcattct aagcaaggat
    12121 tagctcttaa tgcgattgcg caagatgaga tagccgcaaa ttgcttaggc atcaatatcg
    12181 caaaccaaaa gctttttgct tttgttatcg gaagtatttt ctgcggatta gccggttcat
    12241 tattggtggg tttggaagga tatgtcggac ccgatagtta tgatttcagt aaatcattaa
    12301 tgttgatttg tatggtaatt ctcggcggca tggataattc gatcggaatt attgtcgggg
    12361 catttatttt gaccataatc agtgaaaaat tacgtgattt cgctgattat cagcaattag
    12421 tatatagttt ggttcttgtg atcatgttga ttattcggcc gaatggaatt attcccagac
    12481 gtgtgcgtaa atactttaac gctttcagaa gttctcccgt tgcgaaaacg attgaagcag
    12541 agaaagcaca ggctatagat tttaataaat aataaacaaa ctaataattt acaa
//
LOCUS       DAOSMM010000063        12122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_5226_length_12122_cov_16.660479, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000063 DAOSMM010000000
VERSION     DAOSMM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5226_length_12122_cov_16.660479"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..620
                     /gene="rdgB"
                     /locus_tag="PK898_08435"
     CDS             24..620
                     /gene="rdgB"
                     /locus_tag="PK898_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOU20097.1"
                     /translation="MRQKILIATNNLNKIREIQNILSDFDLITPQELGIDLKIEENGN
                     TFEENAEIKAESFCKASGMLSLADDSGLEVECLGGEPGVHSHRYCPKPNATDADRRAF
                     LLENLKGKPRPWKARFYCAAALCDPKSSEIQLVHGICNGEIIPEERGNGGFGYDPIFW
                     IPERGMTLAEMSENEKNEISHRGAAMRKIREILKKKDV"
     gene            627..1844
                     /locus_tag="PK898_08440"
     CDS             627..1844
                     /locus_tag="PK898_08440"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOU20098.1"
                     /translation="MDTIRHLYRIGHGPSSSHTMAPRRAAEMFKARYPDAEHYTVVLY
                     GSLAATGKGHLTDKAIYEVLGKKKTEIIWKPDYVPAFHPNGMEFSAWDGQKQLINKWM
                     VYSVGGGALSEGPQDSEVGLFYPHRNMAEIMAHLQRTGKSFWEYVEDCEGKGIWDYLR
                     EVQKTMYAAIDRGINREGVIPGGLGISRKAPIYFRRGRLMNPTMASVGRLTSYALAVS
                     EENATGGLVVTAPTCGSSGVVPAIIRYLQENLNHSDETILKALATAGLFGNVVKHNGS
                     ISGAEVGCQGEVGVAAAMAAAAAAQLYSCSIRQIEYAAEMALEHHLGLTCDPVHGLVQ
                     VPCIERNAFAAGTAIYVAEYVSFTDGSHLVSFDQVVEVMMQTGVDMPSLYRETSEGGL
                     AKFFKTPKKNSSE"
     gene            1848..2603
                     /locus_tag="PK898_08445"
     CDS             1848..2603
                     /locus_tag="PK898_08445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU20099.1"
                     /translation="MNRDTIQKLSELNRDFYESFGKAFAATRQRIQPGVRRFLDEDCF
                     DGDWLDLGCGSGALAAAWAESGRTGSYTGVDFSQTLLEEARQLNENISDSRMKLSYFQ
                     ADLSDSSWVRLFENRKFDGICAFAVLHHMPDASARSRIFQQVYSLLKPGGCFCYSVWQ
                     FQNSEKMMRRILPWESIGIQSADVDPGDTLLDWRFSLPDADKKIGLRYVHLFTEGELQ
                     SLAEEAGFLLRKEYFSDGASQNMALYRVEQRKE"
     gene            complement(2689..3960)
                     /locus_tag="PK898_08450"
     CDS             complement(2689..3960)
                     /locus_tag="PK898_08450"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU20100.1"
                     /translation="MTDNKKWYFNFGFLWTGQMLSLLSTSIIQFVLLWWLTEKTQSAI
                     YLSAATFVLYIPIIFIVPLISPLIDHCSRKKILIAANFGIGAVSALLGISFGFEKVSP
                     IMVLGAIFLRSTFNSIRWSTLEASAAQMVPESHLTQINAVDYILRGITGIAGPFLGAV
                     LLRTTTINSILNVDLIAAAGGSIPLLLISFPKVNSIATHFSVNLVKQIGRSLSDLKSG
                     FYYVRRTPGLFMFLMYVSITNLMLMPAESLLPLVVSTKFHGTEISMSTIGIAFGIGSV
                     LGGIILSIRHGFRSRIKASLTGDLLYGFGIFLIGVASEKQFSLAVFGWALAGIGESMS
                     LANVNALIQAHTRPEMQGRVFSISTSLINISIPVAMVISGPIAEIFGIQFWYIFSGVG
                     ILLLAGGMMFVPAMFRLEKPSPAKILDPSYE"
     gene            4730..5800
                     /locus_tag="PK898_08455"
     CDS             4730..5800
                     /locus_tag="PK898_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20101.1"
                     /translation="MPAKLRTFFNQIFDAVIPLFATLLALFIGVFVLQALGKDPIAAY
                     KALFDGGFGNQRALIQTTIKATPLLLVGIGVCIAFRGGVINIGGEGQIIVGALAAAAV
                     ALYVPIHSRIMMPICLLASMAAGAVWGGIPGILKAKLAVNEILTTVMMNSIAVYLSNY
                     LLRGPMIDPHEIEMGTRAAQTALLPEYTFLTRLAPPTQLNTGTILALILSVIAFVMLF
                     KTTIGYRIRAVGLNSDASRYAGIKVPFYQALALIMGGAMAGLAGGVEVLGVQHRVLEN
                     ISANYGFNGIVTALFGNLHPLGTIPAAFLFGGLMVGGNKLQRTVQVHSAFIDTLLGLI
                     VLFVVGAEIFSKRRSKKRAING"
     gene            5793..6725
                     /locus_tag="PK898_08460"
     CDS             5793..6725
                     /locus_tag="PK898_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20102.1"
                     /translation="MDNLFSAATIFGIVGMAIKLAMPYLYAGLGELFDQTAGCYNLGT
                     EGIMMMGAFCAFFVVYQTHNLILGVFVAAVVGLLMGLIMSLISVTFKAEQGISGIGLT
                     MFGVGLSSLLFKTLLNGTVLSVDGFKDIRIPVLADIPYVGEALFDHNIMVYIAYLLVP
                     ISSVILYKTTLGLKIRSVGQNPQAADTLGVSVDKIRYLCVCIGGVLAGIAGATFSIAN
                     INMFQEKMTNGTGYIAVALVYFSGWKPKGVLMGALLFSFVNALQLRLQVLGIDIPSDI
                     AMMLPYILTIVTLVFVQSRDAPAALSVPYERSEN"
     gene            6769..7755
                     /locus_tag="PK898_08465"
     CDS             6769..7755
                     /locus_tag="PK898_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOU20103.1"
                     /translation="MFRKNSIILFVVIAVLAVLFVPAFAQDDDVLRVAMVMPSKINDY
                     SFSQSMYEALKKIGESYPEGKFELVYSEDMFNVADAREAIRDYADMGYDLVIAHGSQY
                     GTSLQEIAPDYPETAFAWGNGTDYYGQPNISVYQARAEQGGYVFGQIAAKYTNKGKLG
                     VCGPVESGDAKTYIDGFKYGAEQGGSQVSVTYTSSFSDVSLMAAAAETHMAEGATLLT
                     GTSQSIPGAVSVVKEKGGVWMGVQWDTIPLAPKNVLVSLVYDWTPILNDIIANMKEGV
                     YGGKAYDLTFEDGGLKFVWNTENMEIDPELIKMADELAAKITAGEINPLPAE"
     gene            7885..9447
                     /locus_tag="PK898_08470"
     CDS             7885..9447
                     /locus_tag="PK898_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20104.1"
                     /translation="MTEREGSNQQPNHIKKLQMVDITKQFPGVLANDKVNLEVNEGEI
                     LALLGENGAGKSTLMKQLYGLYKPDSGKILINGEERVFNSPVDAINAGIGMIHQHFML
                     VSNLTVVENVALGLKSSRGLFLDLDVVEKRILEICETYKLKVDPKAYIWQLAVGEQQR
                     VEIIKALYRGADLLILDEPTAVLTPQEVEEFFVILRKMAEAKHSLIFISHKLNEVIEI
                     SDRIVILRDGRSVAEKKNENITKTELAEKMVGRPVVLQYEKTPLNQRDIRLKIVNLHV
                     ENDWGNEKVCGVDLEIHGGEILGLAGVSGNGQPELAQALAGLRKPTQGKIYLNGKDVT
                     DATPYERTLAGQSYIPEERNKDGVIKDFNISENFILEDHDKEPYSKHTFFNFANIARE
                     TQKAVDDFAVKTPNLKTFVKNLSGGNVQKLILARELSRNPSVLIAAQPTRGVDIGASE
                     YIHQRLLKQRDQGTATLLISEDLDEIRALSDRIAVIFEGKIMGIVDAATATSEQLGLM
                     MAGIPQEEAMKQ"
     gene            complement(9472..9909)
                     /locus_tag="PK898_08475"
     CDS             complement(9472..9909)
                     /locus_tag="PK898_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HOU20105.1"
                     /translation="MSKNKLFLFCELTIGIILAVVSFVFRKDMPVIIAGILIAAGIGL
                     IGNSISQLMMDHFEKKNPEVIREDTPFTADERNSLIRYKAKAKTSDITQWLIIGMALI
                     SVLTKAPVWAIILFALVYLAHLLMPYYFMVRYRNEMKNNSPES"
     gene            complement(9940..11580)
                     /locus_tag="PK898_08480"
     CDS             complement(9940..11580)
                     /locus_tag="PK898_08480"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOU20106.1"
                     /translation="MNHYSLKLNQCLTVLPEIRSISGNKPDITYEDITFDSRKVTPGS
                     IYVALKGINTDGHNFIPNAIQNGAVAVIGSNDMPTELPVPYIRVENPRLAMAYAAAAL
                     YRFPSNEMAVIGVTGTDGKTTTATLMYHILRKAGLKAGLISTVSAIINGREIDTGFHV
                     TTPESPDIQRYLAQMRDEGITHVVCEVTSHGLSQNRVAAIDFDIAIVTNITHEHLDYH
                     KTKEEYFAAKGMLFKALGQHPRKLAPLAVLNYDDQASYTYLSDIIPNTHKICYSASGE
                     ELVSQVSLKKVVTFSKGLKAEILFRNLPDHTEDRIVSVQSNLIGTYNCANILAAMTAA
                     VYGLGISPEKAAQGVQEVSAISGRMEVIDCGQNFTAMVDFAHTPNALAVALDSARAML
                     PISEDSGIPPNRIIAVYGSAGLRDREKRRMMPAVSVEKADITILTAEDPRTESLDMIL
                     KEMADEAVKHGAVMGENLFIEPDRGNAIRLGLSLAKPGDIVISFGKGHEQSMCFGTVE
                     YAWDDRTAMRAALCEMLNKEGPQMPFLPTSNRETQIQK"
BASE COUNT         3311 a         2509 c         2975 g         3327 t
ORIGIN      
        1 tttattgata gaatagtgta gacatgagac agaaaatatt gatcgcaacc aataatctta
       61 ataaaatccg ggaaattcaa aatatcctga gtgatttcga tttaattacc ccgcaagagc
      121 tcggaattga tttgaaaatt gaagagaacg gaaatacatt cgaagaaaat gccgaaatca
      181 aagccgaatc tttttgtaag gcgagcggta tgttatcgct ggcagatgat tccggcttgg
      241 aagtggaatg tttgggtgga gaaccgggag ttcattctca ccgatattgc cccaaaccga
      301 atgccaccga tgcggaccgg cgagcgtttt tactcgaaaa tttaaaaggg aaaccgcgtc
      361 cgtggaaagc caggttttat tgtgcggctg ctttatgcga tccgaaatcg tcggaaatcc
      421 aactggttca cggaatttgt aacggcgaga ttattcccga agaacgtgga aacggcggct
      481 tcggctatga tccgattttt tggataccgg aacgcggaat gactttggcg gagatgagtg
      541 aaaatgaaaa aaatgaaatc agtcatcgcg gagcagccat gcgaaaaata cgggaaatac
      601 taaaaaagaa agatgtttga ttaagaatgg atacaattcg ccatttatat cggatcgggc
      661 acggtcccag cagcagtcat accatggcgc ctcgaagagc tgccgagatg tttaaagctc
      721 gttatccgga tgcggaacat tatacggtcg tcttatacgg aagtttggct gcgaccggta
      781 aaggtcactt gaccgataaa gcgatttatg aagttttggg gaaaaagaaa actgaaatta
      841 tttggaaacc ggattatgtt ccggcatttc atccaaacgg gatggaattt tccgcctggg
      901 acggacaaaa gcaattaatt aataagtgga tggtctactc ggtgggcggc ggagcactct
      961 cagaaggtcc gcaagattct gaagtcggtt tattctatcc ccatcgaaac atggcggaaa
     1021 taatggcgca tcttcaacga accgggaagt cattttggga atatgtggaa gattgcgagg
     1081 ggaaagggat ttgggactat ttgcgtgagg tccagaaaac gatgtatgct gcgatcgatc
     1141 gcgggatcaa tcgggaaggt gtcattccgg gcggactggg aatttcccgc aaagctccga
     1201 tctactttcg acggggcaga ttgatgaatc cgacgatggc atccgtaggt cgtttgacct
     1261 cttatgcttt ggctgtttcc gaagaaaacg ctaccggcgg gctggtggtt acggcgccga
     1321 cttgcggatc cagcggggtt gttccggcaa ttattcgcta cttgcaagaa aatttgaatc
     1381 actcggacga aacgattctg aaggctttgg cgacagccgg cttgttcggc aatgtggtaa
     1441 aacataacgg atctatctcc ggtgcagagg taggttgcca aggtgaagtc ggagttgccg
     1501 cggctatggc agcggctgcg gctgcacagc tttattcctg ttctattcgg caaattgaat
     1561 atgctgcgga aatggcgttg gagcatcatt tgggattaac ctgcgatcct gtacacggtc
     1621 tggttcaagt tccttgcatc gagcgaaatg ctttcgctgc cgggaccgcc atctatgtag
     1681 cggagtatgt cagttttaca gacggttcgc atttggtcag ttttgatcaa gtagtagaag
     1741 tcatgatgca gaccggggta gatatgccct ctttataccg tgaaaccagt gaaggcggac
     1801 tggcgaagtt ttttaaaaca cccaaaaaga attcgtcgga ataaagcgtg aaccgagata
     1861 cgatccaaaa actttcggag cttaatcggg atttttatga atcgttcggg aaagcttttg
     1921 cggctacccg gcagcgaata caaccgggag ttcggcgttt tcttgatgag gattgctttg
     1981 acggtgattg gcttgatttg gggtgcggat cgggcgcttt agccgccgca tgggctgaat
     2041 ccggcagaac ggggtcttat accggagtcg atttcagtca gacactgctg gaagaagccc
     2101 ggcaattaaa tgaaaatatt tccgatagcc gcatgaaatt gtcctatttt caggcagatt
     2161 tatcggatag ttcttgggtt cgattatttg aaaacagaaa atttgacgga atttgtgcct
     2221 ttgccgttct ccatcatatg cccgatgcaa gtgcccgaag ccggattttt cagcaagttt
     2281 attctttatt gaagcccggc ggttgcttct gctattcggt ttggcaattt caaaactccg
     2341 aaaaaatgat gagacgcatt ctcccctggg aatcaatcgg aattcaatcc gccgatgtcg
     2401 atcctggcga tacgctgttg gattggcgtt tttcacttcc ggatgcagat aaaaaaatcg
     2461 gactgcggta tgtccacctt tttaccgaag gtgaattgca atcgttggcg gaggaagccg
     2521 gatttctgct tcggaaagaa tatttctcag acggggcaag ccaaaatatg gctttgtacc
     2581 gagttgaaca acggaaagaa taaatggatc ctttttgttt cggttgtgga taacccgatc
     2641 ggcttccttc ccaaatcaaa tcatcatatt gccaagaatt caaataaatc attcatacga
     2701 aggatcaaga attttggcgg gagacggttt ctccaaccga aacatggcag gcacgaacat
     2761 cattcctccg gctaataaca gaattcctac tccgctgaaa atataccaaa attgaattcc
     2821 gaagatttcg gcgatcggtc ccgaaataac cattgccacg ggaatagaaa tattgatcaa
     2881 gcttgtcgag atcgaaaata ctctcccctg catctccggg cgggtatgag cctgtattaa
     2941 agcgttcaca ttcgccagac tcatcgattc accgattccg gccaatgccc atccgaagac
     3001 tgccaacgaa aattgttttt cggaagccac cccgattaaa aagatcccga aaccatacag
     3061 cagatctccg gtaaggctcg ctttgattcg gctgcgaaat ccatggcgta tgctcaaaat
     3121 gatgcccccc aatacggaac cgatgccaaa tgcaataccg atggtactca tggatatttc
     3181 ggtaccgtgg aattttgtcg atacgaccaa aggcaagaga ctctcggcgg gcatgagcat
     3241 caaattggtg atagaaacat acatcaaaaa cataaataag cccggtgttc ttcggacata
     3301 gtaaaaaccg cttttcagat ccgataatga tctgccgatt tgttttacca aatttaccga
     3361 aaaatgggtt gcgattgaat ttactttcgg gaacgaaatc aacaataacg gaatcgagcc
     3421 tcctgccgcc gcaattaagt caacgttcag gatagaattt atcgtcgttg ttcgtaataa
     3481 aacagcgccg aggaacgggc cggcgattcc ggttattccg cgcaaaatat aatcaaccgc
     3541 attgatttgg gttaaatgac tttcggggac catttgtgca gctgaggctt ctaaggttga
     3601 ccagcgaatg ctattaaatg ttgagcgaag gaatatcgct ccgagaacca ttatcgggga
     3661 cactttttcg aagccgaaag atattcccag caaggcagag actgctccga ttccgaaatt
     3721 tgctgctatc agaattttct tgcggctgca atgatcaata agcggactga tcaaaggcac
     3781 aataaagata atcggaatat ataatacgaa agttgccgcg gataaataaa tagccgactg
     3841 tgttttttcc gtcagccacc atagcaaaac gaattgaatg attgatgtgg agagcagtga
     3901 tagcatctgc cccgtccaca ggaatccaaa attgaaatac catttcttgt tgtctgtcat
     3961 aacgtaaatc ctgaaccaat ttgaatacga ttctttttca ggtatgcaaa ccgtaaattc
     4021 attgattgac aaatttttgc aattctcaat caaatgaaaa ggtttaaatc cgaaaaagga
     4081 atccgcagaa attaaaatta aaaacactgc gagattctga tcaatctgcc tttaaggttc
     4141 aaccttaata gggattatgg agtgattggt tcaggtgtaa taaaaagcga atctttggaa
     4201 gatttcacac cagctgcggc gtttaatctc acaactcttt tcatatagtt gattgaagat
     4261 cgtgatggca aaattggtca ggattcgaac gatcttcaca acctgattcc tgttttctgg
     4321 atcttctgtg gttttcatga tgcacccact ttggaaaccg gttagtcgga tgagataggc
     4381 tgatttcccg gtctcctgag aaaaaacctg cctataaaaa gagtatacca ctgatttttg
     4441 tgagattttt gtcaaacggg gttttgtcaa gagggcgatc tgtcttttga tccagcgatc
     4501 atccgatcga ttctaaagta gaatacaata attctttaac ttgtctaacc ttttatgact
     4561 gagagacgct tttcgattaa aactttatta caaaatcatt aattaccggc ctatttttct
     4621 atgtataata ggttttacaa tgaaatgata caattaaagc atatttattt tcagtttttt
     4681 gctttttcac catagtcacg atttttaccg gacggaggaa atatattgaa tgcctgctaa
     4741 attgcgcaca ttcttcaatc agatttttga tgcggtcatt cctctatttg ccactttgtt
     4801 ggcattattt atcggggttt ttgttcttca ggctttggga aaagatccga tcgcagctta
     4861 taaagccctg tttgacggag gattcggcaa tcaacgggca cttattcaaa cgactataaa
     4921 agccaccccg cttcttttgg tgggaatcgg tgtttgtatt gcttttcgcg ggggcgtgat
     4981 caatatcggc ggtgaaggac agattattgt cggagcgttg gcagccgctg ccgtcgcatt
     5041 atatgttccg attcattcca gaatcatgat gccgatctgt ttattagctt cgatggcggc
     5101 gggggctgtt tggggcggaa ttccgggaat cctgaaagcg aaattggcgg tcaatgagat
     5161 tctgacgact gtgatgatga attcaatcgc tgtgtattta tccaactatc ttttacgcgg
     5221 accaatgatc gatccgcacg aaattgagat gggaacacgg gcggcgcaga cggctttatt
     5281 gccggaatac acttttctga caagattggc tcccccgacc cagttaaata ccggtaccat
     5341 tttggcattg attctttctg tcattgcgtt tgttatgctg tttaaaacga cgatcggcta
     5401 tcgaattcga gcggtcggat taaattcgga tgcctcgcgg tacgcaggta ttaaagtccc
     5461 cttttaccag gcattggcat taattatggg tggagctatg gcgggactgg ccggaggagt
     5521 ggaagttttg ggcgtccaac accgggttct ggaaaacatc tcggcaaact acggtttcaa
     5581 cgggattgtt accgcattgt ttggaaattt gcatcctttg ggaacgattc cggctgcctt
     5641 tttattcggc gggctgatgg tcggcggcaa taaattgcaa cgtaccgttc aagttcattc
     5701 ggcttttatt gatacattgc tgggcctaat tgttctgttt gtggtgggtg cggaaatatt
     5761 ttcaaagaga cggtcaaaga agagggcaat caatggataa tttattctct gcagcaacga
     5821 tctttggaat tgtcggaatg gcgataaaac tggcgatgcc ctatctttat gccgggctgg
     5881 gtgaactttt cgatcaaacc gccggttgtt ataacttagg tacagaagga attatgatga
     5941 tgggggcttt ctgtgccttc tttgtcgttt atcagactca caatcttatc ttgggagttt
     6001 tcgttgccgc cgtggtcggg ttgttgatgg gattgattat gtcgttaatc agcgtcactt
     6061 tcaaagccga acagggaatc agcgggatcg gattgacgat gtttggggtc gggctttcct
     6121 ctctgttgtt taagacatta ttaaacggga cagtcttgtc ggtagacgga ttcaaggata
     6181 tccgaatacc ggtgcttgcc gatatcccct atgtgggaga agcgttgttt gatcataata
     6241 taatggttta tatcgcttat ttattagttc cgatcagttc cgttatctta tacaaaacaa
     6301 ccttgggatt aaaaatccgt tcggtcggtc aaaatccgca agccgcagat accctcggcg
     6361 tttccgtaga taaaatccgt tatctttgtg tctgtattgg cggggtactt gccggaattg
     6421 ccggagcgac tttttcgatt gccaatatta atatgttcca agaaaaaatg accaacggga
     6481 ccggttatat tgcggtagct ctggtttatt tcagcggttg gaaaccgaaa ggtgttctga
     6541 tgggagcatt actattcagt tttgttaacg cgctgcaatt gcggcttcag gttttgggaa
     6601 ttgatattcc atccgatatc gcgatgatgt tgccttacat cttgacgatc gtcactctgg
     6661 tatttgtgca gagcagggat gcacccgctg cgctttcggt accgtatgag cgcagtgaga
     6721 attagtcgta ttaatcaaaa ttctggaatt attaaaaagg agttaaaaat gttcagaaaa
     6781 aattcaataa ttttattcgt tgtgattgcg gttttggctg ttctgtttgt tccggcattc
     6841 gcacaagatg atgatgtatt aagagtagcg atggtgatgc cgtcgaagat taatgactac
     6901 tcgttcagcc agtcgatgta tgaagcgctg aaaaaaatcg gtgaatccta tcccgaagga
     6961 aagttcgaat tggtttattc cgaggatatg ttcaacgtag ccgatgcccg ggaagcgatt
     7021 cgagattatg cggatatggg atatgacctt gtgattgctc acggttccca atacgggact
     7081 tccttacagg aaatcgctcc cgattatccg gagaccgcat ttgcttgggg aaatggcacg
     7141 gattattacg ggcagccgaa tatttcggtt tatcaggcac gggctgaaca gggcggatat
     7201 gttttcgggc aaatcgcggc gaaatatacc aataaaggga aattgggtgt ttgcggaccg
     7261 gtcgaatccg gtgatgccaa aacttacatt gatggtttca agtacggtgc cgaacagggt
     7321 gggtcgcagg tcagcgtgac ttatacttcg tcattttcgg atgtctcttt gatggcggca
     7381 gccgcggaaa ctcatatggc ggaaggcgcg acacttttga ccggaacttc tcaatccatc
     7441 cccggagccg tcagcgtggt aaaggaaaag ggcggtgtat ggatgggcgt ccaatgggat
     7501 acgattccgc tcgctccgaa gaatgttttg gtcagcttgg tatatgattg gactccgatc
     7561 ctgaacgata tcattgccaa tatgaaggaa ggagtctacg gcggaaaggc ttatgatttg
     7621 acctttgagg acggcggtct gaaatttgtc tggaatacgg agaatatgga aatcgatccg
     7681 gagctgatta aaatggcgga tgaactggct gcgaaaataa cagccggaga aattaatccg
     7741 ctgccggcgg aatagaggag ccgtatcgat cgtttcatcc atcgtaaaaa atcggaggga
     7801 gcttgccact ccctccgaaa gatgatttag ggtttgattt gaagaaaggc gacggaatgg
     7861 aaatgttgcc ggaaggaatt tgttatgacc gagagggaag gatctaacca acaaccaaac
     7921 catattaaaa agctgcagat ggtggatatt accaaacaat ttccgggtgt cctcgcaaat
     7981 gataaagtca atctggaagt aaacgaagga gagattttag ctttattggg tgaaaacgga
     8041 gccggaaaaa gcaccttaat gaaacagctt tatggtttgt ataaacccga cagcggaaaa
     8101 attttgataa acggagaaga gagagttttt aattcccctg tcgacgcgat aaatgcgggg
     8161 atcgggatga tccatcaaca ttttatgttg gtatctaacc tgaccgttgt tgagaatgtg
     8221 gcgttaggtc tgaaatccag tcggggcctt ttcttagatt tggatgtagt tgaaaaacgg
     8281 atactggaaa tttgcgaaac gtataagcta aaagtcgatc cgaaagccta tatctggcaa
     8341 ttggcggtcg gcgaacaaca acgtgtggaa atcattaaag cgctttatcg cggtgcggat
     8401 cttttgattt tggatgagcc caccgcagta ttgactccgc aagaggtgga agagtttttt
     8461 gtcattttac ggaaaatggc ggaagcaaaa cattctttga tatttatctc gcacaagctg
     8521 aatgaagtga ttgaaatcag cgatcgaatt gttattctca gagatggtcg aagcgttgcg
     8581 gaaaagaaga atgagaatat tacaaagacc gagctggctg aaaaaatggt cggtcgcccg
     8641 gtcgttctcc aatatgagaa gacgccgtta aatcaaaggg atatccgatt aaaaattgtc
     8701 aatttgcatg tcgagaatga ttggggaaat gaaaaggttt gcggtgttga tcttgaaatc
     8761 cacggaggtg aaattctcgg attggccggt gtttccggca acggacaacc cgaacttgct
     8821 caggcattag ccggcctacg caaaccgact caggggaaaa tctacctgaa cggaaaagat
     8881 gtgaccgatg cgacacctta tgaacgaaca ctggccgggc aatcttatat tcccgaagag
     8941 agaaataaag atggtgtgat caaggatttt aatatatccg aaaatttcat tttagaggat
     9001 catgataaag aaccttattc aaaacacact tttttcaatt ttgcgaatat tgcccgcgaa
     9061 actcaaaaag cggttgatga ttttgccgta aaaacaccga atcttaaaac tttcgtcaag
     9121 aatctttccg gaggaaacgt ccagaaatta attctggcgc gggaactcag ccggaatcct
     9181 tcagtattga ttgctgctca gcccactcga ggagtggata tcggagcttc cgaatatatt
     9241 catcaacggc tgctgaagca acgggatcag ggaacggcaa ccctgttgat ttcggaagat
     9301 ctggatgaaa tacgggcatt atccgatcga attgcggtta tctttgaagg aaaaatcatg
     9361 gggatcgtcg atgcagccac tgcaacatca gaacaattgg ggctgatgat ggcgggtatt
     9421 cctcaggaag aagcgatgaa acaataggcc gcacccgttt cggtcgacat ttcaggactc
     9481 gggagaatta tttttcattt cgttccgata gcgaaccata aaatagtacg gcatcaataa
     9541 atgagcaaga taaaccaagg caaataggat aattgcccaa accggagcct tcgtcaatac
     9601 cgaaatcaat gccattccga taataagcca ttgcgttata tcgctcgttt ttgctttcgc
     9661 tttataccgg atcagactgt tccgttcatc ggccgtgaag ggagtgtcct ctcggattac
     9721 ttcgggattc tttttctcga aatggtccat catcaactga ctgatactat ttcctatcaa
     9781 gccgataccc gctgcgatta aaatgccggc aatgataacc ggcatatcct ttcgaaaaac
     9841 aaaagatacg acagccaaaa taattccgat ggttaattca cagaatagaa aaagcttgtt
     9901 cttagacatt tcattccttt caacacagac ggacggatgt tatttttgaa tttgcgtctc
     9961 acgatttgaa gtcggaagga aaggcatctg aggtccctct ttattcagca tttcacagag
    10021 agccgctcgc atagctgttc gatcatccca agcatattca accgttccga agcacatgga
    10081 ttgttcatgg ccttttccga atgaaataac gatatctccc ggtttcgcca gcgaaagtcc
    10141 cagccgaata gcatttcccc gatcgggttc gataaacaga ttttcgccca taaccgcacc
    10201 gtgttttact gcttcgtctg ccatttcctt taggatcata tccagcgatt cggtacgggg
    10261 atcttcggca gtcagaatgg ttatatcggc tttttcaacg gataccgcgg gcatcatccg
    10321 tcttttttca cgatcgcgta atccggctga tccgtaaaca gcgattattc gattcggtgg
    10381 aatgccggaa tcttcggata tcggaagcat tgcgcgggcg gaatcaagag cgacagccaa
    10441 tgcattcggc gtatgggcaa aatcaaccat cgcagtgaaa ttctgaccgc agtcgatcac
    10501 ttccatgcgg cccgaaattg ccgaaacctc ctgaacgcct tgcgccgctt tctccggtga
    10561 tatgcccaat ccgtaaacgg ctgcggtcat cgctgccaaa atatttgcac agttataagt
    10621 accaatcaaa ttggattgaa ccgaaacaat tcggtcttcc gtatgatctg ggagatttcg
    10681 aaacagaatt tccgctttta atcccttcga aaaggtgaca acctttttaa gactcacctg
    10741 agagacaagt tcttctcccg aggctgaata acagatttta tgcgtattgg gaatgatatc
    10801 cgataaatag gtataagaag cttggtcatc atagttcaag accgctaatg gcgctaattt
    10861 cctcggatgc tgaccgagag ctttgaataa catccctttt gccgcaaaat attcttcttt
    10921 cgttttatga taatccaaat gttcgtgagt gatgtttgtc acaattgcga tgtcaaaatc
    10981 aatcgcagcc actcgattct gcgacaatcc gtgagaggtg acttcacata cgacatgggt
    11041 aatcccttcg tctcgcattt gtgccagata ccgttggata tccggacttt ccggtgtcgt
    11101 cacatgaaaa ccggtatcga tctcacgacc gttgataatc gccgataccg ttgagatcaa
    11161 cccggctttc aagccggctt tcctcaaaat atgatacatt aacgtggctg tggttgtttt
    11221 cccgtctgtt ccggtgacac cgatcaccgc catttcattg gaaggaaaac gataaagagc
    11281 cgcggcggca taagccattg ccaagcgcgg attttctaca cggatgtatg gaaccggaag
    11341 ttcggtcggc atatcattcg aaccgattac ggcgacggcg ccgttttgaa tggcattggg
    11401 gatgaaatta tggccgtcgg tattgatccc tttcaaagcg acgtagatgc ttcccggggt
    11461 gactttcctg gaatcaaagg tgatatcttc ataggtgatg tcgggtttgt tgccggagat
    11521 acttctgatt tctggtaaaa cggtaagaca ttggtttaat ttgaggctgt aatgattcat
    11581 tacagttttg cccccttgta tttattttat tgttcttatt ttaccaaatt cactttgtct
    11641 tttcggcaac aatttggcgc gattccctga aagacaattt ttttcaagat gattgtcata
    11701 ccataattgg ttatatgaca ggttaaaaaa ggaggttacc cgtaaagcat gcctaactag
    11761 agagggatgc tttctaaaag tggcgccgag cagccgcgca cagcagttgt gtcgaacgag
    11821 acgtcgcgcc tgacaagcat ggcacgcata gcgggaagcc gtcccctata ggcaaccggt
    11881 gtgattcgca aagacgccgt cctctaaaga gggcgcaact ctataaagtc gatgttttcc
    11941 cctcgccctc agggagaggg tcagggtgag gggaaaacga tgatgcctca cgttgttcgg
    12001 ctcgagatgg catcctcgcc gtcccctacg tttaaacgaa acgggcgggc catatcaata
    12061 gccgggcgaa gtttaccacc catagtcggg ttatgtttac catcaatcat tttaataaaa
    12121 cc
//
LOCUS       DAOSMM010000064        11813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_5387_length_11813_cov_10.540058, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000064 DAOSMM010000000
VERSION     DAOSMM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5387_length_11813_cov_10.540058"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1476)
                     /locus_tag="PK898_08485"
     CDS             complement(163..1476)
                     /locus_tag="PK898_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HOU20107.1"
                     /translation="MENFSSAIPIVDVIRSHKKEKPIGIFSVCSANESVISATLKESK
                     RNDNHFVLIEATCNQVNQFGGYTGMTPFDFADYIREIAYRMDFPLNRIILGGDHLGIN
                     PWQSEPIDSALHKACDMITAFVNAGFAKIHIDMSMPSISDVDLPLTSTLIAERSAILC
                     EAAENASAITDGIRPFYVIGSEVPPPGGSKSNEQTLEITQPKNVRETLEIFEEVFTKM
                     GLSDAFSRTIALVVQPGVEFGDHEIHDYNHENARKLSEAILEFSDIIYEAHSTDYQTV
                     ESLRSMVKDHFAILKVGPELTFAYREAIFALAAMEEEWVGKRYGSETSHVLDIIDQVM
                     LENPSYWIKFYGQDSAQQAFSRKYSLSDRIRYYWNEPHVSAAVLKLIDNLIRYPLPYS
                     LISQFMPAEAQLIREKKITNSPQDLVESHIGTVIRKYQQSCWGDE"
     gene            complement(1502..2431)
                     /locus_tag="PK898_08490"
     CDS             complement(1502..2431)
                     /locus_tag="PK898_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU20108.1"
                     /translation="MQKKFDIAVIGDVNPDLILTGKDIIPEFGQVEKWIDDAELTIGG
                     SAGIFAVGAAKLGLKVTFIGKVGDDHFGHFMKDRMTAQGIDVSGLVIDPSVKTGLSIS
                     LSDGNDRAILTFGGSNLLLNFQDIDFEIVAQSRHLHSGSFYLLNNIRQDLAIVYKKAK
                     EMGLSTSIDTNYDPKEKWEGDIFNVLDYTDVFLPNMTELMAISGEKDPDSAIRHLTKN
                     RNLLVAVKNGEDGAFAEKDKVRYSAETIQVKPVDTTGAGDSFDAGFLFGFLNGLGIEK
                     SLRIGCICGALSTLGIGGTSAQAGREDLTKFGL"
     gene            complement(2493..3344)
                     /gene="fba"
                     /locus_tag="PK898_08495"
     CDS             complement(2493..3344)
                     /gene="fba"
                     /locus_tag="PK898_08495"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF009374.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOU20109.1"
                     /translation="MPLISSFEILSDAQKGHYAVGAFNIENMEMAQAVIKAGEETNSP
                     LIIQTTPSTLKYAPPSLFYAIVKSIAENSKIPVALHLDHGDSFKRCCETIRAGYTSVM
                     IDGSKLPFDENVILTQKVVEVASNLSIPVEAELGTIGGKEDSLVTLDKDALYTNPDQA
                     KEFVDRTRVNSLAVAIGTAHGFYKGVPKLDFERLEEIVKIVNIPIVLHGGSGIPDEDV
                     QKSIRLGISKVNYATELRWHFTQACRKVLEDSDIFDPKKYGTAGREAVKEFVKQRIIV
                     CGSAGKA"
     gene            complement(3382..4419)
                     /locus_tag="PK898_08500"
     CDS             complement(3382..4419)
                     /locus_tag="PK898_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOU20110.1"
                     /translation="MSLTYQEIISQYDALKKTYELFRKHAEELKTFVNSENFSLIAYV
                     GCGSSYSIASGMAVTTQMKLAKPSLSIAGGDLMLHHDRYKKILDGALIVAVSRSGSTS
                     EVLKSINALKNDGCRFKLLTFSAVENSKLSQISDFVFDMPWAFDQSVCQTRTVTNFYL
                     CGVLFAAILDDDQGLLSELKAVIDNGENYLHLFEPVFKEVAGRKWGHGVVLGDAELCG
                     ICEEGALTFKEICQVDSNYYHLLDARHGPMVMIKSDTLVIAVVDDPKNTYERGMIEDI
                     VKKQAVVVVYSDIPCDLEGIINICYGKSLNPISRGIPFILIPQLINYYKSAFTGADPD
                     KPEGLDPWIKL"
     gene            complement(4515..5564)
                     /locus_tag="PK898_08505"
     CDS             complement(4515..5564)
                     /locus_tag="PK898_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU20111.1"
                     /translation="MNTEILDIRNYYPISKLEITKTEINKPKFPVIDAHNHLGTTFGG
                     NWIHKPLEDLITVMDQAGIVSMVDLDGFEGESVFEEHLKKIKEKAPDRFRIYTGPDYS
                     KWEEEKDNFGEYAAKKLQQQIRQGAQGLKIWKNLGAHVRDHRGARVRLCDPRLDPLWH
                     MAAEYHLPVTIHFADPVAFYDPLDEKNERWEELHAHPEWHFPSPPFPSFQQLYDDFLS
                     LLQRNPDTLFIGAHVGCYAENLSVVEKTLREFPNYYIDISERIAELGRQPYTAREFMI
                     KNADRIVFGLDRPANISEYRCYYRFLESKDEYFDYSEEEIYRQGRWKIYGIFLPDDVL
                     EKIYRKNAEKILVPN"
     gene            complement(5598..7583)
                     /locus_tag="PK898_08510"
     CDS             complement(5598..7583)
                     /locus_tag="PK898_08510"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOU20112.1"
                     /translation="MTIQISSIQIPVTKILDDNPLPFFRDRKDREILDRGLLPEEKKN
                     FAQNTGFRVLPYLMQDNYESELKETQLETIILENEFLKAVFLPTLGGRLYSLWDKQKN
                     KELLFSNPVIRMGNLALRNAWFSGGIEWNFGHYGHTFLTCEPMFFARLIDEKGEPFLR
                     MYEFERCKGLFFQVDFHLPAGSKNLIAHFRIMNPSDKSVPIFLWTNIAVKEVDGCRVF
                     SGTEEIIIQVQSNKPGTSYFSHSQLPDPFQEGIDHSYPLNIHHSEEYYFQNDPSHAYE
                     AVVYPDGETFYERSTGNYPYRKMFCWGNHAGGKRWQDQLSLPGQGGYLEIQSGMFRTQ
                     QHSGEISAKQTISITQLFGSGEVDYQEANGQYRQSRMLIERKIEDWLPEERIDLEHRR
                     CCTLADKKAGTILHNGSGWGALEKKRNTLFVPFHLDFPLSTMSKAQQSWIDLLEKDIF
                     RESPSFMISAEWIEIMKKALSEDPKNTHLLTHLGIAFYENERYEDAKNYWEKAVAIQP
                     NPLALRNLAFLSASEGDFNNACALMEEAIQLLSEPERAYAEEYILWLTNAKKSQKAFD
                     YYKSLPVNLQESERILLNIAFSAFEIGKEEFLDRLFSRSFATIREGDTRLIDLWIRYS
                     AQKEAKKQGVMITDQFIDNFRKTVKIPKNLDFRMVND"
     gene            complement(7587..8465)
                     /locus_tag="PK898_08515"
     CDS             complement(7587..8465)
                     /locus_tag="PK898_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU20113.1"
                     /translation="MGRTRFTRNISWKKEARLFPGYLILTLWVIFTVVLIGWVFAASF
                     ATTREIFKGQALKFPTGFHFENYVKAWTSQNVSAFFLNSLGYASISSVLLILICAPAA
                     YVLSRFKFFLNHTIQNSFVAAMGVPMVMIILPLFGVVTQLGLLKQDITVRILMIILYV
                     GINVPYTTIFLLAFFSNLSSSYEEAAAIDGCPPMKIFWKIMLPLAQPGIITVSIFNFI
                     NVWNEYFMSLIFANNDRVKPVAVGLYSMIDSMKYTGDWAGMFAAVIIVFLPTFIIYLF
                     LSEKIIAGITAGGLKG"
     gene            complement(8475..9380)
                     /locus_tag="PK898_08520"
     CDS             complement(8475..9380)
                     /locus_tag="PK898_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU20114.1"
                     /translation="MKRNKTMIVVFLAPALIIFCAIFIYPILRTIVMSLFKVENVTSN
                     PDQWIFVGIENYKTLIHTSLFQIALWNLARIWLIGGIVVLSLALLFAVILNSGIRFKK
                     FYRAVIYMPNIISAVALATMWLQFVYNPKFGLLTTTFKSLGLDHLASINWTDSDHKFW
                     ALLFAYCFGMVGYHMLIFSSGIEKIPQELFEASTIDGASKTQQFKSITFPLLRSVIKT
                     NITMWSITSVAFFVWSQLFSMVTADTQTITPMVYMYLQVFGAGNVISERNAGLGAAVG
                     VLMGLCVVIVFFLTNRVIKEDDLEY"
     gene            complement(9463..10701)
                     /locus_tag="PK898_08525"
     CDS             complement(9463..10701)
                     /locus_tag="PK898_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20115.1"
                     /translation="MKKRMFCLVLALIVLLSITGAVFAEGKTIVYWSMWSASEPQAIV
                     ISEAVKSYEAASGNKVDLQFKGRTGIREGLQPALDAGTVIDLFDEDIDRVNVTWGDYL
                     MDLEDLAKASDYEKTAIPGLIAACREAGGGRLKSIPYQPNIFEVFYNQDIFKEAGIDT
                     VPTTWEEFLADSEKIKAAGYIPFTTDDAYVLTNFGYHLARYIGQDGVLDVVNNGKWAE
                     TPEVLKAAQDYETLYKNGYLSPTFGIAAWPTNQNGEFALGDAAMYLNGSWLPNEVKGI
                     TGPDFLWGCFAYPTVPGGKDGLEAANYGAQVFAINKNSKVAEEAFEIIKTITKGEFDK
                     KLSVESVGIPADTTNTEWPAMLASVKEVMAKLTTRYQWSAGIENNPNMTPIIKENFQK
                     LCTGKETAQEFVDALEAASK"
     gene            10908..11765
                     /locus_tag="PK898_08530"
     CDS             10908..11765
                     /locus_tag="PK898_08530"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOU20116.1"
                     /translation="MIYNLNTSRLNELLINLQEITDLKFSLHDLKAHELYTVNKRSAF
                     CNLICDTQDGYSHCYGSDLQAINKLEGKTSPYQYRCHAGLIDTAIPITENGQLIAVIL
                     FGQLLDDSPIDEQWQKTKKLCQWYTDQSRLQEAFYKLPQLSQRNIRAAYEIVNACVSE
                     VRLEKIVEFDNLPDSQRLQSFINAHYASHLSLDEISYEMAISKSKLCKIAREVDKNMT
                     VGKLITQKRIEVAKKKLIESDMPIREISEIVGIPDFNYFTKVFKRATGSTPSEYRSHF
                     SEQAVHPEV"
BASE COUNT         3577 a         2596 c         2099 g         3541 t
ORIGIN      
        1 tcgagagtgc cgataatgat tctaagtggg taacgttaga ttaaacgaag catcaaatat
       61 taacaaaaag aggtctgagg ttgaaaaagg tctttccgac ctcagaccct tttattaatc
      121 tctgcctccg gaatgaatct gatagatttg taaccggtct gattactcat ccccccaaca
      181 actctgctga tattttcgaa taaccgttcc gatatgagat tcaaccaaat cctgaggcga
      241 attcgttatt tttttctccc gaataagttg tgcttccgcc ggcataaatt gactgattaa
      301 tgaatacgga agcggatatc gaatcaaatt gtcgattaat tttaggacgg cagccgatac
      361 atgcggttca ttccagtagt aacgtattcg atcacttaag ctatactttc ttgaaaaagc
      421 ttgctgagca gaatcttgtc cataaaactt aatccaataa gacggatttt ctaacataac
      481 ctgatcaatt atatcgagta catgactggt ttccgatccg tatcgttttc caacccattc
      541 ttcttccatt gccgctaatg caaaaatagc ttcacgataa gcaaaagtca attccggtcc
      601 tacctttaga attgcgaaat gatccttgac catcgatcgg agtgattcaa ctgtttgata
      661 atccgtagaa tgagcttcat atattatgtc agagaattcc aagatagctt ctgaaagttt
      721 tcgggcattt tcatgattat aatcatgaat ttcatggtca ccgaattcca ctccgggctg
      781 caccaccaaa gcaatggttc tggaaaacgc gtcactcaaa cccattttgg taaaaacttc
      841 ttcaaaaatt tcaagagttt ctctcacatt cttcggttgg gtaatttcta atgtttgttc
      901 attgcttttt gatcctccgg gtggcggaac ttccgaaccg ataacataaa agggtctaat
      961 tccgtctgtg attgcggagg cattttctgc agcttcgcat aaaatggcag acctctcggc
     1021 aataagggtc gatgtgaggg gcaaatcgac atcacttatc gaaggcatac tcatatcaat
     1081 atggatttta gcgaagccgg cattgacaaa agcagtgatc atatcacatg ccttatgtaa
     1141 tgccgaatca attggttcgc tttgccaagg gttgattccc aaatgatccc caccgagaat
     1201 tatcctattc agagggaaat ccatacgata agcgatttca cggatatagt ccgcaaaatc
     1261 gaagggcgtc atacctgtat aaccgccaaa ctgattaacc tgattgcatg tagcttcaat
     1321 taatacaaag tgattatcat tccgttttga ctctttcagc gtcgcgctga ttacggattc
     1381 gttagcggaa caaaccgaaa aaatcccgat aggtttctct tttttatgag aacgtataac
     1441 atcaacaatc ggaatcgcag aactaaaatt ttccattatt ccttccatag atacaaattt
     1501 gttacaaacc gaatttagtt aaatcttctc taccggcttg tgcagatgtt cctccgatac
     1561 ccaatgtgga taatgcaccg cagatgcagc cgattcttaa acttttttca attcctaatc
     1621 cgtttaagaa accaaacaaa aaacctgcgt caaatgaatc accggcaccg gttgtgtcaa
     1681 caggctttac ctgaattgtc tccgctgaat aacgcacttt atctttttcg gcaaaagcgc
     1741 cgtcttcacc gttctttacc gcaaccaaaa gattacgatt cttcgtcaaa tgccggatcg
     1801 ccgaatccgg atctttttct ccggaaattg ccattaattc agtcatattc ggcaaaaaaa
     1861 catccgtata gtccaataca ttaaaaatat cgccttccca tttttctttg ggatcataat
     1921 ttgtatcaat ggaggttgac agccccattt cttttgcttt tttgtatact atagccaaat
     1981 cttgacgaat attatttaaa aggtaaaacg atcctgaatg taaatgccgg ctctgggcaa
     2041 caatctcaaa atcgatatcc tgaaaattga ggagcaaatt tgatcctccg aatgtcaaaa
     2101 ttgcacggtc attcccgtca gataaactga tgctcagacc ggttttaaca gacggatcaa
     2161 ttaccagacc gctgacatcg atgccttgag ccgtcattcg atccttcata aaatgtccga
     2221 agtgatcatc accgactttg ccgataaaag ttactttcaa accgagtttt gcggctccga
     2281 cagcgaatat tcctgccgat cctccgatgg tcaactcggc atcatcgatc catttctcaa
     2341 cctgaccgaa ttccggaata atgtctttcc cggttaatat cagatccgga ttaacatccc
     2401 caattactgc gatatcaaat tttttttgca tgattattcc gtttagcaaa ctttccttta
     2461 tcggatattt ctattaagat ttaaaaaagt cctcaggctt ttccggcaga accgcaaaca
     2521 ataattcttt gcttgacaaa ctctttaacc gcctcacgcc ctgcggtgcc atatttcttc
     2581 gggtcaaaaa tatccgaatc ttccaggact tttctgcaag cttgagtaaa atgccagcgg
     2641 agttcggtgg cataattaac tttgctaata cctaagcgaa tggatttttg aacatcttca
     2701 tccggaatcc cggatcctcc gtgcaacaca attggaatat tgacaatctt gacaatttct
     2761 tccaaccttt caaaatcaag tttgggaact cctttataaa aaccatgagc cgtaccaata
     2821 gccactgcca aactgttaac cctcgtacga tcgacaaatt cctttgcctg atccggattc
     2881 gtataaagtg catccttatc caatgtgacc agactgtctt cttttccccc aatggtgcct
     2941 aattccgctt cgacgggaat tgataagttc gatgcaactt caacaacctt ttgagttaaa
     3001 atgacattct catcaaacgg aagttttgat ccgtcaatca ttaccgacgt atacccggct
     3061 cggattgttt cgcaacaacg cttaaatgaa tccccgtggt ctaaatgtag cgcgacagga
     3121 atttttgaat tttcggctat cgatttcaca atcgcataaa aaagtgaagg aggtgcatat
     3181 tttaaagttg aaggtgtcgt ctgaatgatt aaaggcgaat tggtttcttc tccggctttt
     3241 attacagcct gagccatctc catgttctct atattaaaag cgccgacagc ataatgccct
     3301 ttttgagcat cagataaaat ttcaaatgaa ctgataagtg gcataaaacc tcaattccga
     3361 attattgtaa aatctctcta tctataactt gatccatgga tccagccctt cgggtttatc
     3421 aggatccgca ccggtaaaag ccgatttata ataattgatc aattgcggaa tcaaaataaa
     3481 aggaattccg cgactgatcg gattcagact tttgccataa caaatattaa taatcccttc
     3541 caaatcgcag ggaatatctg aatatacaac cacaaccgct tgtttcttga caatatcttc
     3601 gatcattcct cgttcataag tgtttttcgg atcatccaca accgcaataa caagcgtatc
     3661 agatttgatc atcaccatcg gcccatgacg ggcgtctaat aaatgataat aattggagtc
     3721 gacttgacaa atttctttga aagtcaaggc accttcttcg caaattccac agagttcggc
     3781 atctcccaga acaacaccat gaccccattt tctccccgca acctctttaa ataccggttc
     3841 aaataaatgt aaataattct ctccattatc gattactgct ttcagctcac tcaacaatcc
     3901 ttgatcatcg tcgaggattg ctgcaaataa tacaccgcaa aggtaaaaat tcgtaaccgt
     3961 tcgagtctga cagacacttt ggtcaaacgc ccatggcata tcgaaaacaa agtctgaaat
     4021 ttgcgataat tttgaatttt caacggcaga aaaagtaaga agtttaaatc ggcaaccgtc
     4081 attttttaag gcattaatgg actttaggac ttcactggtg cttccggaac gggaaaccgc
     4141 aacgattagt gccccatcaa gaattttttt ataacgatca tgatgaagca taaggtcacc
     4201 gccggcaata gaaagcgaag gcttagcaag tttcatttgt gtcgttacag ccatacctga
     4261 tgctatacta tagcttgatc cgcaaccgac atatgcaatc agagaaaaat tttcggaatt
     4321 cacaaaagtc ttgagttctt ccgcatgttt ccgaaataat tcatatgttt tcttaagcgc
     4381 atcgtattgt gaaataatct cttgataggt taagctcatt ttatattcct ttctcaacaa
     4441 tctcatccag atatcagatt cggttcatca ttgaaactca atgaatctga caagataatc
     4501 atcgaatcct gcgattaatt cggaaccaat attttttccg catttttccg gtaaattttc
     4561 tctaaaacat catcaggcaa aaaaatgcca taaatcttcc aacgtccctg acgataaatt
     4621 tcttcttcgc tgtaatcaaa atattcatct tttgattcta agaatcgata ataacaacgg
     4681 tattccgata tatttgccgg gcgatccaat ccaaagacaa ttcgatcggc attttttatc
     4741 ataaactcac gagcggtata cggttgtcga cctaattcag cgattctttc ggaaatgtca
     4801 atgtaataat tcggaaactc tcggagtgtt ttttcaacca ccgacagatt ttccgcataa
     4861 catcctacat gggcccctat aaacagcgtg tcgggatttc tttgtaataa actcaagaaa
     4921 tcatcataca attgttgaaa agagggaaat ggcggggaag gaaaatgcca ttccggatga
     4981 gcatgcagtt cctcccatcg ctcatttttt tcatctaaag ggtcataaaa ggcgacggga
     5041 tcagcgaaat gaatcgttac cggtaaatga tattcggctg ccatatgcca taacggatcc
     5101 aacctcggat cacataaacg aacacgtgcc ccgcgatgat cccgaacatg cgctcccaaa
     5161 ttcttccaga tttttaatcc ttgggcgccc tgccgaattt gttgttgaag tttttttgct
     5221 gcatattccc caaaattatc tttttcttct tcccactttg aataatccgg tcccgtatag
     5281 atgcgaaatc gatccggagc cttttctttg atcttcttta aatgttcttc aaaaaccgac
     5341 tctccttcga aaccatccaa gtcaaccatg gaaacaattc ctgcttgatc catgaccgtg
     5401 attagatctt ccaacggttt atgtatccag tttcctccga atgttgttcc cagatggtta
     5461 tgtgcatcga tcaccgggaa tttaggctta ttgatctcgg tttttgtgat ctcaagtttg
     5521 gaaatcggat aatagtttct gatatccaga atctcagtat tcatatttgt tttaagtgct
     5581 tcattattat tcatcgttta atcattcacc attctgaaat caagattttt gggaattttt
     5641 acagttttcc ggaaattatc aataaactga tctgtaatca ttaccccttg tttttttgct
     5701 tctttttgtg cagaatatcg aatccataaa tcaatcagtc tcgtatcacc ctcacggata
     5761 gtcgcaaacg acctggaaaa taaacgatcg agaaattctt ccttaccgat ttcgaaggca
     5821 ctgaaagcga tgtttaatag gattctttcg gattcctgta aattgacagg taaagatttg
     5881 taataatcaa aagctttttg cgattttttt gcatttgtta accacaggat atattcttcg
     5941 gcataagccc tctcgggttc cgaaagcagc tgtattgctt cttccatcaa tgcacatgcg
     6001 ttattgaaat ctccttccga cgcagaaaga aaagccaaat ttcgtaaagc aagagggttc
     6061 ggttgaatag cgacggcttt ttcccaataa ttctttgcat cttcatatcg ttcgttttca
     6121 tagaatgcaa tccccagatg ggtcaaaaga tgggtatttt tcgggtcttc tgacaatgct
     6181 tttttcataa tttctatcca ttcggcactg atcataaagc ttggactttc tcgaaaaata
     6241 tctttttcaa gcaaatctat ccaactctgc tgtgctttgc tcatcgttga gagaggaaaa
     6301 tctaaatgaa agggaacaaa taaagtgttt cgcttttttt ccagtgctcc ccaacctgac
     6361 ccgttgtgca aaatcgttcc tgctttcttg tccgctaacg tacaacaccg gcgatgttcc
     6421 agatcaattc gttcctcggg taaccaatcc tcaatttttc gctcaatcaa cattcgagat
     6481 tgtcgatatt gaccgtttgc ttcttgatag tctacctctc cggaaccaaa aagttgggta
     6541 atagaaatag tttgtttcgc tgagatttct ccggaatgtt gttgggtccg aaacatcccc
     6601 gattgtattt ccaaatatcc gccttgccca ggtaagctta gttgatcttg ccaacgtttt
     6661 ccacccgcat gatttcccca acaaaacatt ttccgatacg gataattacc cgttgaacgc
     6721 tcataaaaag tttctccatc gggatataca actgcttcat aagcatgaga cggatcattt
     6781 tgaaaataat attcttcgga atgatgaata tttaaaggat atgaatgatc aattccttcc
     6841 tggaaaggat ccggtaactg actatgggaa aaataacttg tgccgggttt attgctttgt
     6901 acctgaataa taatttcttc ggttccggaa aatacgcggc aaccgtccac ttccttaacg
     6961 gcgatatttg tccacaaaaa tatcggtacg gatttatccg acggattcat aatccggaaa
     7021 tgggcaatta aatttttgga tccggcaggc agatgaaaat caacttgaaa gaaaaggcct
     7081 ttgcaccgtt caaattcata catccgcaga aaaggctctc ctttttcatc tattaagcgt
     7141 gcaaaaaaca tgggttcgca ggtcagaaag gtatggccgt aatgtccgaa attccactcg
     7201 attccgccgg aaaaccaagc gtttcgtaac gctaaattcc ccattctaat taccggatta
     7261 gaaaatagca gctccttatt tttttgttta tcccataatg aatagagtcg cccgccgaga
     7321 gtcggtaaaa aaaccgcttt taagaattca ttttcaagaa taatcgtttc taattgtgtc
     7381 tcttttaatt cagattcata attgtcttgc attaaatagg gtaaaacgcg aaatccggtg
     7441 ttttgtgcaa aatttttttt ctcttccggc aacaatccgc gatctaaaat ctcgcgatcc
     7501 ttccgatctc tgaaaaaagg cagagggtta tcatccagta ttttcgttac cggtatttga
     7561 atagaactta tctgaattgt cattgtttat cctttcaaac ctcctgctgt aataccggca
     7621 attatttttt cggataggaa taaatagata ataaaagtcg gtaagaatac gataatcaca
     7681 gccgcaaaca taccggccca atctcctgta tacttcattg aatcaatcat tgaatataaa
     7741 ccgaccgcaa ccggttttac acgatcatta tttgcaaaga tcagagacat aaagtactca
     7801 ttccaaacat taataaaatt gaaaatgctg accgttataa ttccgggctg tgccaaaggc
     7861 agcataattt tccaaaatat tttcatcgga gggcagccgt cgattgccgc tgcttcttca
     7921 taagagctgg aaagattgga aaaaaaagcc agtaagaata ttgtggtata aggaacatta
     7981 attcccacat atagaataat catcaatatt cgtaccgtta tatcttgctt caacaatccc
     8041 agctgagtta cgactccgaa tagcggcaga ataatcatca ccatgggaac gcccatagcc
     8101 gcgacaaaac tgttttgaat cgtatgattc aagaaaaatt taaatcgaga taaaacataa
     8161 gctgccggcg cgcaaatcaa tatcaataaa acagaggaaa tagaagcata tccgagagag
     8221 tttaggaaaa aagccgatac attttgagat gtccatgctt taacgtaatt ttcgaaatgg
     8281 aatccggttg gaaatttcaa agcttggcct ttaaaaatct ctcgtgtcgt tgcaaaactg
     8341 gcggcaaaaa cccagccgat cagaacaacc gtaaaaataa cccagagtgt caagattaag
     8401 taacccggaa acaaacgggc ttcttttttc cagctaatat ttctcgtgaa tcgagttctt
     8461 cccattattt cctcctaata ttccagatca tcctctttaa ttacccgatt cgtcaagaaa
     8521 aagactataa ccacacaaag acccattaaa acgcccacag cggcacctaa gccggcattt
     8581 cgctcactga ttacatttcc ggctccaaaa acctgtaaat acatataaac catcggtgtt
     8641 attgtttgcg tatccgccgt aaccattgag aatagctgtg accaaacaaa gaatgcaacg
     8701 gaagtaatag accacatcgt tatatttgtt ttaatcacac tacgcaacaa cggaaaggtt
     8761 atgcttttaa attgctgtgt tttcgaagca ccgtcaatgg ttgaagcttc gaataattcc
     8821 tgaggaattt tctcaatgcc gcttgaaaat atcagcatat ggtatccgac cattccgaaa
     8881 caataagcaa acaacaacgc ccaaaattta tgatcggaat cggtccaatt gatcgaagca
     8941 aggtggtcca gaccaagaga tttgaatgtg gtggttaata aaccgaattt tggattgtag
     9001 acaaactgga gccacatcgt cgccagtgca acagcactga tgatgttagg catatatata
     9061 acagcccgat aaaatttttt aaaccgaata ccgctgttta agattactgc aaagagtaaa
     9121 gccaagctca aaacaactat tccgccgatt aaccagattc tagccaggtt ccacaaagct
     9181 atttgaaaga gactggtatg gatcaaggtt ttgtaatttt caatcccgac aaaaatccat
     9241 tggtccggat tggatgtcac gttttcaact ttaaataagc tcatgacaat cgtacggaga
     9301 atcggataaa taaatatcgc acaaaaaata atcagagcag gagctaaaaa aacaacgatc
     9361 attgttttgt tccttttcat cgtttgttcc ctcctttctt tgcgctaaaa gaaaaagggg
     9421 tgaaagccga taaagctttc aacccctttt cccaatcaaa tcttatttac tggccgcttc
     9481 taaggcgtca acaaattctt gagccgtctc tttgccggtg cagagtttct gaaaattctc
     9541 tttaataatc ggcgtcatat tcggattatt ttcgattcca gccgaccatt gataacgggt
     9601 ggttaatttt gccatgactt ctttcacaga cgccagcatc gcaggccact ctgtgttggt
     9661 tgtatccgcc ggaattccga ccgattctac cgaaagtttt ttgtcaaatt caccttttgt
     9721 tattgttttg ataatttcaa acgcttcttc ggcaacctta gagtttttgt tgattgcaaa
     9781 aacctgcgcg ccatagttgg ctgcttctaa tccgtctttt cctcccggga cagtcggata
     9841 agcaaaacaa ccccataaaa agtcggggcc ggtgattccc ttcacctcat tcggcaacca
     9901 ggacccattc aaatacatag ctgcatcacc caaagcaaat tcgccgtttt gatttgtcgg
     9961 ccaagcggca attccaaaag tcggagagag atagccattc ttataaaggg tttcatagtc
    10021 ctgggctgct ttcaatactt ccggcgtttc tgcccattta ccgttattga cgacatccaa
    10081 aacaccgtct tgtccaatat aacgagccaa atgataaccg aaattcgtca atacatacgc
    10141 atcgtcagtc gtaaaaggaa tatatccggc agctttgatt ttttcactgt cagctaagaa
    10201 ttcctcccat gtggtgggca ctgtatcaat accggcttct ttgaaaatgt cctggttata
    10261 aaatacttca aatatattcg gctgataagg tattgatttt aatcttcctc cgcccgcttc
    10321 gcgacaagcg gcaattaatc ccggaatggc tgttttttca taatcggaag ctttggcaag
    10381 atcctccaaa tccatcagat aatcacccca agttacattg acccggtcaa tatcttcatc
    10441 aaaaagatca atgaccgtgc cggcatctaa tgccggttga agtccttcac gaattccggt
    10501 ccggccttta aattgcagat cgactttatt ccccgatgcg gcttcatacg atttgaccgc
    10561 ttcagaaatt acaatcgcct gaggttcgct ggcagaccac atcgaccaat aaacgattgt
    10621 ttttccttca gcaaagacag cgccggttat tgataacagc acaataagtg ctaaaaccaa
    10681 acaaaacatt cttttcttca tctgattctc cttttgtttt tttcagttca tttacaaaat
    10741 gcaacgaact gattaatatg tacacccatt tgtataaacg tttattaatt gcattatatg
    10801 aaaagctttt taaaacgaat agaaaaatcg cttgtattaa tagtaaaatc tattattatt
    10861 ttaccaataa ttctattctg ttattatagt tagttgaacg ggaaaatatg atctataacc
    10921 ttaatacctc aagattaaat gaattgctga ttaatttaca ggaaataact gatctaaaat
    10981 tttctctgca tgatttaaaa gcacatgaac tttataccgt caacaaaaga agcgcttttt
    11041 gcaacctcat ctgtgacact caagacggat atagtcattg ttacggctcc gatttgcaag
    11101 ctatcaataa attggaagga aaaacctctc cttatcaata tcgatgtcat gccggattga
    11161 tcgatacggc tattccgata accgaaaacg gtcaattaat tgcggttatt ttatttggac
    11221 aattattaga cgattcaccc attgatgaac aatggcagaa aacaaaaaag ttatgtcaat
    11281 ggtatacaga tcaatcaaga ttacaggagg ctttctataa attgcctcag ttatctcaac
    11341 gcaacatccg agcggcatat gagatcgtaa acgcttgtgt cagtgaagta agattggaaa
    11401 aaatagtaga attcgataat cttccggatt ctcaaaggct tcaatccttt attaatgctc
    11461 attacgcttc tcatttgtcg ttggatgaaa tatcctatga aatggcgata tcaaagagca
    11521 aactttgcaa aatagcgaga gaagtcgata aaaatatgac cgtcggaaag ttgatcactc
    11581 aaaaacggat tgaagtggca aaaaagaaac taatcgaatc cgatatgccg atccgagaaa
    11641 tttctgaaat agtcggaatt ccggatttta attatttcac aaaagttttc aaacgagcga
    11701 ccggttccac cccgagtgaa tatcgcagtc atttttctga acaagcagtt catccggagg
    11761 tctgattgat cgtctctcgg atttttactg aaataaaaag atcgggatcg ttc
//
LOCUS       DAOSMM010000065        11452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_5632_length_11452_cov_14.938229, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000065 DAOSMM010000000
VERSION     DAOSMM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5632_length_11452_cov_14.938229"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PK898_08535"
     CDS             complement(<1..623)
                     /locus_tag="PK898_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOU20117.1"
                     /translation="MKGFVTIDNQKVEINGERNLLEMIRKTGIDLPTFCYHSELSIYG
                     ACRLCVVETDKGQVISSCSTPPANGMVIKTNTSRLMRIRKMMLELLLANHDRNCTTCP
                     KSGKCKLQELSNRFGIDEIRFPTAEKNRPIDKSSISVARNENKCILCGDCVRTCAEIQ
                     GIGAIDFANRGARTRVMSAFDRGLGETECINCGQCVAVCPTGALTTPS"
     gene            complement(626..2512)
                     /locus_tag="PK898_08540"
     CDS             complement(626..2512)
                     /locus_tag="PK898_08540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOU20118.1"
                     /translation="MTSICSMVELVEKSKLYNHALSKQTLRISVCAGTGCIANGSKFV
                     YEKFLETAKDNKDFVDIHMLTEESVSEGKTIVRTGCRGFCSKGPLVHIDPLNVLYTHV
                     QPEDVTEIYEAALDGKIIERLVYHDPITNEAYPNAEESPFYKNQTSNILLHCGKINPE
                     SIKEYIADQGYFAISKVLTEMTPEQVNNIVSASGLRGRGGGGFPTGTKWNFARVQKAD
                     KKYIVCNGDEGDPGAFMNRSVLEGDPHRVVEGMMIAGYAIGADEGVFYVRAEYPLAIE
                     RLKIAIREAEENGLLGKNILGSGFNFKLSIREGAGAFVCGEETALLASVEGKRGIPRP
                     RPPFPAVSGLYGKPTVINNVETLANLPRIILNGPEWFRSYGTEKSPGTKTFALTGKVR
                     NTGLVEIPMGTTLRQLVFDIGGGIPNGKKFKAVQIGGPSGGCLPDSMLDNPLDYDSLQ
                     KAGAMIGSGGIVVMDEDNCIVEVAKFFMTFIQAESCGKCVACREGTKQMLYIMEKIVN
                     DQATLEDLKLLEEIAHVIQDASLCALGKTAANPILSTLRYFRSEYLAHIVDHQCPAGV
                     CNAFKKYQINPDLCKGCGACAKKCPVNAISGTIKSPFTIDSAICIRCGTCKDTCRFGA
                     IEVR"
     gene            complement(2509..3015)
                     /locus_tag="PK898_08545"
     CDS             complement(2509..3015)
                     /locus_tag="PK898_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU20119.1"
                     /translation="MDELSKDKIISFTQEVALRHNYNRSEVIGMLQEIQEHYHYLPES
                     ALNELSKCLKVTPQTIYGIATFYAQFSLDPKGKYIVKICDGTACHVKNSRKIYDAVEK
                     KMKFIPGKRTTVDGLFTLETVSCLGACGIAPVMVINDKVYAQMDPEAAAGVIETLAKQ
                     ETESAEEQ"
     gene            3366..5030
                     /gene="atpA"
                     /locus_tag="PK898_08550"
     CDS             3366..5030
                     /gene="atpA"
                     /locus_tag="PK898_08550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOU20120.1"
                     /translation="MTEETIRNITADLFEKIDDFQPNYEVEDIGIVMEVGEGIARVSG
                     LPTIGSQELVEFENGARGIAFSLEKDTIGVIVLGNYFTIKEGSIVKKTGRIASIPVGE
                     GLLGRVINPLGEPIDGKGPIRFDGYRAIERIAPGIHERSDVNTPLQTGIKAIDSMIPI
                     GRGQRELIIGDRQTGKTAIAIDTIINQRDKNVYCIYVAIGQKKSTVAQIIAKLEETDA
                     MKHTIIIVAGADDNASMQYIAPYAGCAISEDFMEKGKDALVIYDDLTKHAWAYRQISL
                     LLRRPPGREAYPGDLFYLHSRLLERSAKLDEKYVIVPKSFAGERADLSDSVDHVLFSG
                     PLAYKDAQTALSQKADKENYSVARLAGSGGSSTALPIIETLLGDVSAYVPTNVISITD
                     GQIYLESGLFNAGIRPAINVGISVSRVGGAAQSDAMKKVSGGLRLDMAAYHEIASFAQ
                     FGSDLDKSTRKQLERGEILQEVLKQPQYSPYSVSDEFLIIYAGTHGYIDGVPLNRVAE
                     WEEEFLKFMSASFPSIGKNIEQTGDLTPEIESDLQKAIEKFKLVWK"
     gene            5034..5921
                     /gene="atpG"
                     /locus_tag="PK898_08555"
     CDS             5034..5921
                     /gene="atpG"
                     /locus_tag="PK898_08555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOU20121.1"
                     /translation="MASNREMQNRIRTVESIGQLTRALQAVSASQVRVVRVLSENTLP
                     YTTLSWQVLTDLYHEQEFYNQIRAFQLPDVSKDVLIVFISADRGLAGSYPVNIFREVN
                     HLERKIQTKTKYITIGKKGRDMLQRRRKNIIADFSELSTATSFHDLKTVSEIVIDEFN
                     QERVGQVYLVYTEYESISRFTPTTKRLLPFVDPFLGQLDEKEEKHIRKGGCIFEGDPQ
                     VILKSLITRFIRMELFHAVTSSKASEHTMRMLAMNQATENSEELLQSLQLKYNKIRQE
                     NITKDILDIMGGSEVHSDE"
     gene            5961..7358
                     /gene="atpD"
                     /locus_tag="PK898_08560"
     CDS             5961..7358
                     /gene="atpD"
                     /locus_tag="PK898_08560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOU20122.1"
                     /translation="MKKPTGTVIQVNGSVVDVEFPPNDLPEIFEALEIIKPNGEILVL
                     EVELLLKGFIARCVAMGSTDGLQRSVPVQRTNYPIMVPVGKPALGRVMNVLGRPIDGK
                     GAIESDFYYPLHHAGPDFSHQSTKVELLETGIKVIDLIAPFTRGGKTGIFGGAGVGKT
                     VVIMELIRSIAAVHKGLSVFAGVGERTREGTELLREMTDSGVMKDTVMVFGQMNEPPG
                     VRLRVALAGLANAEYFRNQGMDVLLFIDNIFRFSLAGSEVSALMGNMPSAVGYQPTLA
                     NEMGELQERITSTTLGSITSMQAVYVPADDYSDPAPVTTFAHLDATIALERSIAEKAI
                     YPAVDPLSSGSRILDPNIVGEEHYKIAREVQRILQRYKDLQDIIAILGIDEISEEDKV
                     IVNRARKAEQFFSQPMYVAERYTGIPGAYVRIEDTIEGFRQILSGELDDLPEQAFHMV
                     GTIEEAKAKAQSMNQ"
     gene            7371..7802
                     /gene="atpC"
                     /locus_tag="PK898_08565"
     CDS             7371..7802
                     /gene="atpC"
                     /locus_tag="PK898_08565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOU20123.1"
                     /translation="MNCQIVSRYNVVYEGPADLVLLPSVNGQLGILRNHVRMYTKLQP
                     GIITVRLAGEDTFYTATGGIAEVKPDVVRILVDSSENVDEIDVERALEAKKRAEEAMK
                     NAPSRNSTEFMRAMMEMKKSNLRISASQRRQMHRGTRNMQP"
     gene            7956..8963
                     /locus_tag="PK898_08570"
     CDS             7956..8963
                     /locus_tag="PK898_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU20124.1"
                     /translation="MALFSRELGIDLGTLSLVIAEGKQLLLQEPTIAAIIVEEMKMVE
                     WGQAAKDMVGRVPESIEVVRPLRNGVIAEYEITENLLRFAIQKVSGSMLVFRPRILIT
                     IPCGITSVERRAVHEVGLGTNSREVLLMEQPLAAAIGVDLPIATPSGNMIICLGGGLT
                     QAAVLAMNGIVTSETTQTAGLKLDESIQLYVRKKYGVIIGQPTAEQIKIRIGSAVQGE
                     QPQSMEVQGQDQVSGLPKPVMLTTDDVVEALQDSLAEISNTARKVLEKTPPELISDII
                     DRGVALCGGTSLLRGIDKYLTKSLGIPAYMVDEPTICTGLGAAKAISMRDVYRRSILQ
                     G"
     gene            complement(9030..9653)
                     /locus_tag="PK898_08575"
     CDS             complement(9030..9653)
                     /locus_tag="PK898_08575"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOU20125.1"
                     /translation="MTTVNKAEIPDIVVSRLPRYLRVLKNLRETKKTTNSTELGKLLG
                     YSSAQVRKDFSQFGEFGKQGTGYNIDYLINQFQQILQVNQTWDIVLIGVGNLGRAILN
                     YRGFEKHGFFIRGAFDDDPHIIGEKIGNKVVRDIAEVGDFIKEFDIKIAMIATPASEA
                     QSVAEMLVANNIKAILTYASTILNLPPEIRVEYSDPIISLQHISYYL"
     gene            complement(9650..10354)
                     /locus_tag="PK898_08580"
     CDS             complement(9650..10354)
                     /locus_tag="PK898_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOU20126.1"
                     /translation="MSYISTYLIYAIIPMLLGLWAQAKVQGAFSTYSKVRTRSGITGS
                     QAARKMLDDKGLSDVAIERVSGNLTDHYDPTKRVLRLSEGVYDSPSIAAVGVACHESG
                     HAYQHADAYSWLMLRTGIVPLVSIGSRLGPILFMVGLILAGMMGNFGYNIATLGLIIF
                     SLTAVFSIITLPVELNASHRAKEWIETNGWMVGDEQEGVSKVLSAAALTYVAAAIQSI
                     ANVLYYASFLNRNRRQ"
     gene            10554..>11452
                     /locus_tag="PK898_08585"
     CDS             10554..>11452
                     /locus_tag="PK898_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20127.1"
                     /translation="MAKAQNKSDKILKLCTVIVFIIFGITGLLIYDDYGVTLDEPYER
                     RTGLVNLKFILQDLMHKPLPEELIAIPELGSYEDQDYGFILQFPTILFEYFTNFKHDI
                     SSIYQLRHLWTFLNFYISVIFFNLLLYQRFRNHLISLLGTLFLIISPRIFADGFYNTK
                     DLLFLSWFTISLFFLYRFIHQPTKRNSLVLAIAIALAANTRIIGYILLIVACVYLLCI
                     FFRKEMDWKEFFTNGLILIGVNILLWFLFFPASWGKPIDFLMQSLHHFSNNSSGSQEL
                     YLGKFEPSMHLPWHYLPVWIFIST"
BASE COUNT         3215 a         2334 c         2580 g         3323 t
ORIGIN      
        1 gagggggttg tcaaagcgcc ggttgggcaa accgcgacac attgaccgca atttatacat
       61 tccgtttccc ctaatccccg atcaaatgcg ctcatcacgc gtgttcttgc accgcgattc
      121 gcaaaatcga tcgctccgat tccctggatt tcggcacatg ttcgaacaca atccccgcaa
      181 agaatgcatt tattttcgtt gcgagctaca ctgatactgc ttttatcaat cggccgattt
      241 ttttctgcgg tcggaaaacg aatttcatca ataccgaatc gattacttaa ttcctgaagt
      301 ttacactttc cgcttttcgg acacgtggtg caattgcggt cgtgatttgc gaggagtagc
      361 tcaagcatca tctttcgaat gcgcatcaag cgagatgtat ttgttttaat taccatgcca
      421 ttcgcaggcg gcgttgaaca ggaactgatc acctgacctt tgtctgtttc cacgacacaa
      481 agacgacaag cgccataaat acttaattcg gagtgatagc aaaatgtggg aagatcaatt
      541 ccggtctttc gaatcatctc gagtaagttt ctttcgccat taatttcaac tttttggttg
      601 tcgattgtta caaaaccctt catttttacc tcacttcgat agcgccaaac cggcaagtat
      661 ctttgcaggt accacaacga atacagatag cggaatcgat tgtgaacgga cttttgatcg
      721 ttccggaaat cgcattcacc ggacattttt tggcacaggc gccacatcct ttgcaaagat
      781 cgggattaat ttggtatttc ttgaaagcat tgcaaacacc ggcgggacac tgatgatcaa
      841 caatgtgagc caaatattcg ctgcgaaaat aacgtaaagt actgagaatc ggatttgcag
      901 ccgtttttcc caaagcgcat aaagatgcat cttgaatcac atgagcgatt tcctcaagta
      961 atttcaaatc ttcgagagtt gcctggtcgt ttacgatctt ttccataata taaagcattt
     1021 gcttggtgcc ttctcggcag gcgacgcatt ttccgcaaga ttcggcttga ataaatgtca
     1081 taaaaaactt agcgacttct acaatgcagt tgtcttcatc cattacgaca attccgcccg
     1141 aaccgatcat cgctcccgct ttttggaggg agtcataatc caacggatta tccagcatcg
     1201 aatccggtaa gcatcctccg gaaggtccgc cgatttgaac cgctttaaac ttctttccgt
     1261 tggggatacc gccgccgata tcgaatacca attgacgaag agtcgttccc atgggaattt
     1321 cgaccaagcc ggtatttctg acctttccgg tcagagcgaa ggtttttgta ccgggacttt
     1381 tttctgtccc ataggatctg aaccattcgg gaccgtttaa aataatcctg ggaagattgg
     1441 cgagtgtctc gacattattg ataacggtcg gttttccata aagtcccgac acagcgggga
     1501 atggtggtcg aggacgagga attccccgtt tcccttctac ggatgccaaa agggcggtct
     1561 cttctccgca tacaaatgcc cccgcacctt cccgaatcga taatttaaaa ttgaaaccgc
     1621 tgccgaggat attcttccct aacagtccgt tttcttccgc ttcacggatc gcaattttta
     1681 atctttcaat tgccagagga tattctgcac gaacgtaaaa aaccccttca tcggcgccga
     1741 tggcatatcc ggcaatcatc atcccttcga ctactctgtg gggatcacct tccaaaaccg
     1801 atcggttcat aaacgcaccg gggtctcctt catcaccgtt gcagacaata tattttttat
     1861 ccgctttttg gacgcgggca aaattccatt tggttccggt cggaaatcct ccgcctccgc
     1921 ggccgcgcag accggaagcg gaaacaatgt tgtttacctg ttcgggagtc atttcagtca
     1981 aaactttcga aatagcaaaa tatccctgat ctgcaatata ttctttaatg gattccggat
     2041 tgattttccc acaatgaaga aggatattcg atgtttggtt tttatagaaa ggtgactctt
     2101 ccgcattggg ataagcttca ttggttatcg gatcgtggta gaccagtctt tcaataattt
     2161 ttccgtcaag agcggcttcg tatatttccg ttacatcttc cggttggaca tgagtgtata
     2221 aaacatttaa tggatcgata tgaaccagag ggccttttga acagaaaccg cggcatcctg
     2281 ttcgaacaat tgtcttccct tcggataccg actcttcggt aagcatatga atatcgacaa
     2341 aatccttatt gtctttagcg gtttccaaaa atttctcata tacaaatttc gatccgtttg
     2401 caatacatcc cgtaccggcg cagacagaaa ttctgagagt ttgttttgat aaagcatgat
     2461 tgtaaagttt gcttttttca actaattcaa ccattgaaca gatagacgtc attgttcctc
     2521 cgctgattct gtttcctgtt ttgcaagtgt ttcaatgacg cctgccgccg cctcagggtc
     2581 catctgagca tataccttgt cattgattac catgacgggt gcgattccgc acgcgcccag
     2641 gcatgatacc gtttcgagcg taaacagacc atcgacggtc gttcttttcc cgggaatgaa
     2701 tttcatcttt ttttctaccg catcatatat ttttcttgaa tttttcacat ggcaagctgt
     2761 tccgtcacaa atttttacga tatatttgcc tttgggatcg agtgaaaatt gagcgtagaa
     2821 agtcgcgatg ccgtaaatgg tctgaggagt gactttcagg cattttgaca attcatttaa
     2881 agcactttca ggaaggtaat gataatgttc ctgaatttcc tgcagcattc caattacttc
     2941 cgaacggttg taattgtggc gcaaggctac ttcctgggta aagcttatta ttttatcttt
     3001 actcaattca tccattagaa ctccatttta aattccttgt gaatttgtta actagcgtgg
     3061 tttattctaa atgataaaac catatttgac aaggtgtaaa aatatcttaa tgtgatttcg
     3121 ggtaaccgtc acagattctt aaagttcaaa aaaatatata ttatttcttt cgcctttgga
     3181 attattttaa aaatggtaaa ataagctcgt tcagattctc acgaatcagc tttgacgtaa
     3241 tacattttcg gtgcgacgga ttcaacgttg atcagaaact ctataatata tgaggtaagg
     3301 aaatgcattt gaaaatggaa ttatgagaaa gaaaacaaac catcgaaatg taattctgag
     3361 gatttatgac agaagaaact atccgaaata taaccgctga tctttttgaa aaaatcgatg
     3421 actttcaacc caattatgag gtggaagata tcggaatcgt tatggaagtc ggtgagggaa
     3481 tcgcacgcgt aagcggtttg cccacaatcg gcagtcagga actcgttgaa tttgaaaacg
     3541 gagccagagg aattgcattc agtcttgaaa aagatacaat cggagtgatt gttttaggca
     3601 actactttac aatcaaggag ggttccattg taaaaaagac ggggcgtatc gcttcgattc
     3661 cggtcgggga ggggttgttg gggcgggtta ttaatccgct tggcgagccg attgacggaa
     3721 aaggtccgat tcgattcgac ggatatcgtg cgattgaacg gattgcgccg ggaattcatg
     3781 agagatccga tgtgaatacg cctttgcaaa ccggtatcaa agcgattgat tctatgattc
     3841 ccattggccg gggtcagcga gaattgatta tcggggatcg tcaaaccgga aaaacagcca
     3901 tcgcaatcga tacgattatc aatcaacggg ataagaatgt ttattgtatt tatgtggcaa
     3961 tcggtcagaa gaaatcgacg gtagctcaaa tcatcgcaaa actcgaagaa acagatgcga
     4021 tgaagcatac gattattatt gtcgccggcg ccgatgacaa cgcttcgatg caatatattg
     4081 ccccttatgc cggatgtgca atcagtgaag attttatgga aaagggaaag gatgcgttgg
     4141 tcatttacga tgacttgacc aaacatgcct gggcataccg ccaaatttcg ctgttattaa
     4201 ggcgtccgcc cggccgagaa gcttacccgg gagatctttt ttatcttcat tccagattat
     4261 tggagcgttc cgcaaagttg gatgaaaaat atgtgatcgt tccgaaatca tttgccggtg
     4321 aacgggctga tctgtctgat tctgtcgatc atgtattatt ttccggtcct cttgcttata
     4381 aggatgccca aacagcttta tcacaaaaag cagataaaga gaattattcc gttgctcgat
     4441 tagccggatc gggcggaagt tcaaccgctt tgcccattat tgaaacgttg ctgggagacg
     4501 tttcggctta tgttccgacc aatgtgattt ctattaccga cggacaaatt tatcttgaat
     4561 cgggattatt taatgcgggt attcgtcccg ctattaatgt ggggatatct gtttcccgtg
     4621 taggcggtgc ggcacaatcc gatgcgatga aaaaggtttc cggcggtttg aggttggata
     4681 tggcggccta tcatgaaata gcctccttcg ctcaattcgg ttccgatttg gacaaatcaa
     4741 cgagaaaaca attggaacgg ggagaaatat tacaggaagt actcaaacaa ccgcaatatt
     4801 ctccttattc cgtcagtgat gaatttctta ttatttatgc cggaacacac ggatatattg
     4861 acggggtgcc tttaaatcga gttgccgagt gggaagaaga gtttttaaaa tttatgagcg
     4921 catctttccc ttcaatcggc aaaaatatcg aacaaacagg tgatctgact cctgaaatcg
     4981 aatccgatct tcaaaaagca atcgagaagt ttaagctggt gtggaaataa aaaatggcat
     5041 caaaccgtga aatgcagaat cggattcgaa ccgttgaatc catcggccaa ttaacgcgtg
     5101 ctttacaggc tgtcagtgca agtcaggttc gtgtagttag agtattgtcc gaaaacactt
     5161 taccttatac aactctatct tggcaggtat tgaccgatct gtaccatgaa caggaatttt
     5221 ataatcagat tcgcgctttt caattacccg atgtttcgaa agatgttttg attgtcttta
     5281 tcagcgccga tcgggggttg gcgggaagtt atccggttaa catatttcga gaggtgaatc
     5341 atctggaaag aaaaattcaa accaagacga aatatattac gatcgggaaa aaaggcaggg
     5401 atatgctgca aaggcgaaga aaaaatatta ttgccgattt cagcgaattg tcgaccgcga
     5461 ccagctttca tgatttaaaa accgtatccg aaatagttat cgacgaattc aatcaggaaa
     5521 gagtcgggca agtatacctt gtttataccg agtatgaaag catatctcgt tttacaccga
     5581 caaccaaaag attacttccg ttcgtcgacc cgtttttagg acaattggat gaaaaagagg
     5641 agaaacacat tcgaaaaggg ggatgtatct ttgaaggaga tcctcaagta attttgaagt
     5701 cgcttataac ccgctttatt cggatggaat tgtttcatgc ggtgacatcc tctaaagcaa
     5761 gtgaacatac gatgagaatg ttagccatga accaagccac tgagaattcg gaagaattgc
     5821 ttcaatcctt acagttaaaa tacaataaaa tccggcaaga gaatataacg aaggatattt
     5881 tggatataat ggggggctct gaggttcatt ctgatgagta aggtttattt taccgaagaa
     5941 attttgtttg ggagaattaa atgaaaaaac cgactggaac tgtaattcag gtaaatggaa
     6001 gtgtggtcga tgtcgaattc ccgccgaacg atttacctga gatttttgag gctctggaga
     6061 tcataaaacc gaacggagag attttagtac ttgaagttga attgcttttg aaaggtttta
     6121 ttgcgcgctg cgttgcgatg ggatcaacgg acggacttca gcgcagtgtt ccggtccaaa
     6181 ggacaaatta tccgattatg gttccggtgg ggaagccggc tctcggaagg gtaatgaacg
     6241 tattaggcag accgattgac gggaaagggg ccatcgaatc cgatttttat tatccccttc
     6301 atcatgccgg tcccgatttc agccatcagt cgacgaaagt ggagttgttg gaaaccggga
     6361 ttaaagtaat tgatttaatt gccccgttta cccgaggcgg gaaaacaggg atttttggcg
     6421 gagcgggcgt aggaaagacc gtagtgatca tggaattgat ccgaagtatc gccgcagtcc
     6481 acaaaggact atcagttttt gccggggtcg gagaaagaac ccgcgagggg acggaactct
     6541 tgcgtgaaat gaccgattcc ggtgtcatga aagataccgt aatggttttc ggtcaaatga
     6601 atgaaccgcc gggggttcgg ctgcgggtcg ctttagccgg tttggcaaat gcggaatatt
     6661 ttcgcaatca agggatggat gttctcttgt ttatcgataa tatttttcgg ttttcattag
     6721 ccggctccga agtttcggca ttgatgggaa atatgccatc cgccgtcggt tatcaaccga
     6781 ccttggccaa tgaaatgggc gaactgcagg aacggatcac atcgactacc ttaggctcga
     6841 ttacctcgat gcaggctgtc tatgtgccgg ccgatgatta ttccgatccg gcaccggtga
     6901 caacttttgc ccacttggat gccaccattg cgttggagag gagtattgcc gaaaaagcga
     6961 tttatccggc ggtggatccg ctttcgtccg ggtccagaat tttggatccc aatatcgtcg
     7021 gtgaagagca ttataaaatc gccagggaag ttcaacggat ccttcagcgt tataaggatc
     7081 tgcaggatat catcgcgatt ttggggattg acgaaatttc ggaagaagat aaagtgattg
     7141 tcaaccgggc caggaaggcc gaacagtttt tctctcaacc gatgtatgtt gccgaacggt
     7201 ataccggaat tcccggtgca tatgtccgta tcgaagatac gatagaaggc tttcgacaaa
     7261 tattatccgg tgaattggat gatctcccgg aacaagcctt tcatatggtc ggcacgattg
     7321 aagaagcgaa agccaaagct caatcgatga atcaataata ggatactaca gtgaattgtc
     7381 agattgtttc gcgatataac gttgtatatg aaggcccggc ggatttggtt ttgttgccga
     7441 gtgtcaacgg acaactggga atattaagaa atcatgtacg aatgtataca aaacttcaac
     7501 ccggtattat aacagtccgt ttggccggtg aggatacctt ttacaccgct accgggggca
     7561 ttgcggaagt taaaccggat gttgtgcgta ttttggttga ttcatcggaa aatgtcgatg
     7621 aaattgatgt cgaacgggct ttagaggcaa agaaacgggc ggaagaagct atgaaaaatg
     7681 ctccttcacg gaattcaacc gaatttatgc gcgctatgat ggagatgaaa aaatcaaacc
     7741 tgcggatcag cgccagccag agaaggcaaa tgcatcgcgg taccagaaat atgcagccgt
     7801 aatgtcgccg cttttttcag agatccgctg catggaaaaa atcggtggtt acttcccgac
     7861 cgatattatt tggtttcaac cgtatcaaac attggtttat gatataaatt atataagatg
     7921 aaataccgat ttttcagaac tggataatta ttaatatggc gcttttttct cgagaacttg
     7981 gaatagattt aggaacgtta agcttggtga tcgctgaagg gaaacaactt cttttgcaag
     8041 agcccacgat cgcagctatc attgttgagg aaatgaaaat ggtcgaatgg ggacaagctg
     8101 caaaggatat ggttggccgt gttcccgaaa gcattgaagt cgtgcgtccg cttcgaaacg
     8161 gtgtgattgc cgaatatgaa atcaccgaaa atctgttacg ttttgccatt cagaaagtca
     8221 gcgggtctat gctggtattt cgtccgagga ttcttatcac aattccttgc ggaataacca
     8281 gtgtcgaacg gcgagcggtt catgaagtgg gactgggaac gaacagccgt gaagtattat
     8341 tgatggagca gcccttggcc gctgcgatcg gagttgattt gccgatcgca accccctccg
     8401 gaaatatgat tatttgtttg ggtgggggac tgacccaagc tgccgtatta gccatgaatg
     8461 ggatcgtcac ttccgaaaca actcagacag ccggtcttaa gctcgatgaa tcgattcaat
     8521 tgtatgttcg aaagaaatac ggggtaatta tcggtcagcc tacagcagaa caaataaaga
     8581 tacggatcgg ttccgccgtg caaggcgaac agccgcaatc catggaggtg cagggacagg
     8641 atcaagtatc cggacttccc aaaccggtaa tgttgacgac agatgatgtg gttgaagctt
     8701 tgcaggattc cttagcagaa atatccaata cggcgcgcaa agtattggaa aaaacacctc
     8761 ccgaactgat ttcggatatt attgatcgcg gagtggcatt atgcggaggg acctcattgc
     8821 tgagaggaat cgataaatat ctgacgaaat cgttgggaat tccggcttat atggtggatg
     8881 agccgacaat ttgcaccggc ttaggagctg ccaaagcgat ctccatgcgc gatgtctaca
     8941 gacgatcaat attgcaagga taaatcacgg cattataaat aaaagaaacg gagagttgat
     9001 ctccgtttct tttatttact caataaagat taaagataat aggaaatgtg ttgcagcgag
     9061 ataatcgggt cactatattc cactcggatt tcaggcggca aattcagaat cgtcgatgca
     9121 taggtcagaa tcgcttttat gttatttgcc actaacattt cggccaccga ttgcgcttct
     9181 gatgcgggtg ttgcgatcat agcaattttt atatcgaatt ctttgatgaa atcacccact
     9241 tcggcgatat ctctgaccac tttgtttcct attttctcgc cgataatatg cggatcgtca
     9301 tcgaaagcac ccctgatgaa gaagccgtgt ttttcaaatc cccgataatt taaaatggcc
     9361 cgccccaaat tcccgacacc gattaaaacg atatcccagg tttgatttac ctgaagaatt
     9421 tgttgaaatt gattaatcaa gtaatcaata ttgtaaccgg taccttgttt accgaattca
     9481 ccgaactgag aaaaatcctt tctgacctgt gcggaagaat atccgagaag tttccccaat
     9541 tcggtggaat ttgtggtttt tttcgtttcg cgtaaatttt tcagaactct taaatatctg
     9601 gggagtctgc tgacgacaat atcgggaatt tctgccttat ttacagtggt cattgacgcc
     9661 tgttacgatt caaaaaagac gcatagtaaa ggacattagc gatcgattga atagcggcag
     9721 ccacataagt aagtgctgcg gcacttaaaa ctttcgatac accttcttgc tcgtctccta
     9781 ccatccatcc gtttgtttca atccattctt ttgcgcgatg gctggcattg agttctacag
     9841 gaagggtaat aatagagaaa accgctgtaa gactgaagat aattaatccc aatgtcgcga
     9901 tgttataacc gaaattgccc atcataccgg ctaaaatcaa gccgaccatg aataatatcg
     9961 gtcccaaacg gcttccgata cttaccagcg ggacgatccc tgttctcaac atcagccaac
    10021 tataagcgtc ggcatgttga taggcatgtc cggattcgtg acaggcaact cccacagctg
    10081 cgatactggg cgaatcatat acaccttcgc tgagtcgtaa aacacgtttt gtcggatcat
    10141 aatgatcggt taaatttccg gaaacgcgtt caatagccac atcggataac cctttatcat
    10201 ccaacatttt ccgtgcggct tgcgaaccgg taataccgga tctggttctg acttttgagt
    10261 aagtagaaaa tgctccttgt acttttgctt gcgcccaaag accgaggagc atcggaataa
    10321 ttgcataaat taaataagtt gatatatagg acatatttcc tccgaaaata attatactgg
    10381 aaatatttta ttgctatcga tatcttattc aagatttata tatattataa tttccttcga
    10441 tcgaggaata attgaaaatc ttgttaacaa aatgagatat gtttattctc ctaaatatgt
    10501 ttaagtaaat aaaattgccg gttgagaaaa agtgattttt caggaaattt tggatggcta
    10561 aagctcaaaa taaatccgat aagattctaa aattatgcac cgttattgtt tttatcattt
    10621 ttgggatcac gggattatta atatatgatg attacggtgt gacgcttgat gagccatatg
    10681 aaagaagaac cggtcttgtc aacctgaaat ttattttaca ggatttgatg cacaaacctc
    10741 ttccggaaga attaatcgcg ataccggaat tagggagcta tgaagatcaa gattatggtt
    10801 ttattttaca atttccgaca attctttttg agtatttcac gaatttcaag catgatatca
    10861 gctcgattta tcaattaagg catttatgga cttttctgaa tttttatatt tcagtgattt
    10921 ttttcaacct tcttttgtat cagcgatttc gaaatcattt gatttcactt ttaggaacat
    10981 tattcctgat catttcaccg cggatattcg ctgatggttt ttataataca aaagaccttt
    11041 tatttctgtc ttggtttaca atcagcttgt tttttcttta tcgatttatt catcaaccga
    11101 caaagcggaa cagcttggta ttagcgatcg caattgcttt agctgcaaat acgcgaatta
    11161 tcggatatat tcttttgatt gttgcttgtg tttatctgct atgtatattc tttcggaaag
    11221 aaatggattg gaaagagttt ttcacgaacg gattgatatt aatcggagtc aatattcttc
    11281 tctggttttt gttctttccg gcttcatggg ggaagccgat cgattttctg atgcaatcgc
    11341 ttcaccattt ctcaaataat agttccggga gtcaagagtt atacttggga aaatttgaac
    11401 caagcatgca tttgccatgg cattatcttc cggtttggat ttttatttcg ac
//
LOCUS       DAOSMM010000066        10558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_6288_length_10558_cov_18.188232, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000066 DAOSMM010000000
VERSION     DAOSMM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6288_length_10558_cov_18.188232"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..515
                     /gene="arfB"
                     /locus_tag="PK898_08590"
     CDS             90..515
                     /gene="arfB"
                     /locus_tag="PK898_08590"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOU20128.1"
                     /translation="MIEISPNVKINENEIQFNFIRGSGPGGQNINKVASSVQLRFDVR
                     NSTSLDPEIKQRLIKLAGNRINDQGVLIIDAKRFRTQEQNRFDATLRLIDLIRKASIT
                     PKKRKATHPSVTAKAARRGEKKKHSETKRLRRYIPEDWE"
     gene            712..798
                     /locus_tag="PK898_08595"
     tRNA            712..798
                     /locus_tag="PK898_08595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:746..748,aa:Leu,seq:cag)
     gene            904..1278
                     /locus_tag="PK898_08600"
     CDS             904..1278
                     /locus_tag="PK898_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20129.1"
                     /translation="MKKWLLTKKQIWLVILVLVVVSMLIDLNTRISTLTYLRDQKNTL
                     AVEVTALQSTLDLVSEKIDYAGSDAAVESWARQQGLMRQEGDYVIIPLPVDEPTPIPT
                     PVPTVEPVKYENWEIWKALIFD"
     gene            1325..2203
                     /locus_tag="PK898_08605"
     CDS             1325..2203
                     /locus_tag="PK898_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOU20130.1"
                     /translation="MTAVIIDGKKIAEGIRNEVGIAVKERIAAGLSVPGLATVLVGND
                     PASQIYVRNKMKACDRLGIYSVSYTLPEDTDFETLKELINQLNVAPEIHGILVQLPLP
                     PQIDEYSVLSMINPQKDVDGFHPINAGMLARKESEPWFIPCTSAGILRLIHSVQPDIT
                     GMEAVVIGRSSIVGMPTAQLLCRNNATVTICHTKTVNLAAVCRRADLLVVAAGHSKLV
                     KGDWVKPGAIVIDVGINRVNTPDSPKGYILTGDTEFDEIVNIAKAITPVPGGVGPMTI
                     AMLMQNTLLAASRYSA"
     gene            2355..3743
                     /locus_tag="PK898_08610"
     CDS             2355..3743
                     /locus_tag="PK898_08610"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOU20131.1"
                     /translation="MSTLLIKNALVLATMDDNRQEISGGGLLIRDGFIESVGKTSDLP
                     AEADEILDLSGCVVLPGLINTHHHFYQTLTRAIPGAQNSNLFNWLKTLYPIWGRMTPE
                     DIYISTQTAMAELALSGCTTASDHLYLFPNGSKLDDEIEGARQVGLRFHASRGSMSLG
                     ESKGGLPPDSVVDSEEHILEDCVRVVEKYHDPKPGSMLQIVFAPCSPFSVSTELMRES
                     AKLARHYGVHLHTHLAETEDEEAFTLEKFGLRPVDYMASVDWLGNDVWFAHSVHVSPE
                     DIQVYKKYGCGVAHCPSSNMRLGSGFAPLMEYLREGIHVGLGVDGSASNDGSHMLAEV
                     RQALLMARLRAGLMGASLSGENAPKLITAREVLEIATRGGAAVLGRKDIGSLEPGKCG
                     DFIAVNMNRLRYTGSLTDPLSALVFCNPGWVDFNYVQGKAIVKNGELMTVNVPDLVQR
                     HNKAAAGLVRNY"
     gene            3757..4497
                     /locus_tag="PK898_08615"
     CDS             3757..4497
                     /locus_tag="PK898_08615"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU20132.1"
                     /translation="MKNQENNYENLSLFQKLNNDLAKIIEETPPGTRLKSEPELAASL
                     GVSRATLREAMRTFEGQGLIRRRQGVGTFVLDQKQNIESGLEVLESIETQAKRLGLDV
                     KMGYLEINHIKTDEELAKHFGIQQGDPVIRISRVIWVADRPVAYLIDNVLPDVLEEED
                     LDKGFTGSVLDLFLRRGKPELSHSKTLVAAFPATTAIAKKMQLQRGDCLLRLEAYLYD
                     KKDRMIDYSLSYFIPGFFKFQVIRSIGK"
     gene            4502..6538
                     /locus_tag="PK898_08620"
     CDS             4502..6538
                     /locus_tag="PK898_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20133.1"
                     /translation="MTNKSSSGSNRPSLPVLFTLAILIAYGVPVFLGWFSFYGDDWIY
                     IYEYHLNGTAGFPAFVAWDRPYSAWIYILATSLFGEHVFPYHLLLLLQRWLAVFLFFK
                     ILQMVWSDIPRVVSGAVLLFAVYPGFHQQPIAVQYILHFASLDLCLFSIWCMLKAFDS
                     GFLEKQSWKGILLTIAGFFSSLTALFSCEYFIGLELVRPFLLWFRQVNRFKDEPVKKP
                     VKIGNIFFHWFPYLLSMMIFIVWRVFIFSFQTYQPKLLNKFNENPAAALSEILRKILT
                     DLYTVTIAAWKKSLNQPAGKENQVIFWLLILVCFFLFAYSLRRFHNREGNKQPAAIEN
                     EKIQFLITGLVALLAAGIPYWVTMINIELDFPWDRPTISFSIGVAMLISVGISFIFQN
                     KFQTLVTASLIAFAIGSHYTNALVYRNEAEKMNDYFWQLAWRAPQLQPGTILVSEQIP
                     LDRYSDNDLTPIVNWQYAPALKGNTYQYKYFDLDLRSETYFRDLRPGQPIVHDYRSHH
                     FESDSSHVLALFYKKGSCLWTITESEAEYPGLPENIVHVAGISDINLIRSDSAVQAVP
                     PSPIGKEPDHGYCYYFQEINRAFQNHDRETAYQAADTALKMGLSSQDPLDFIPAVKAL
                     ILGEDWSRAAELSKKVIMNPENLPFLCEQISGIPETVNQPEYTEMLKTIGCVTQ"
     gene            6574..7830
                     /gene="thrC"
                     /locus_tag="PK898_08625"
     CDS             6574..7830
                     /gene="thrC"
                     /locus_tag="PK898_08625"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU20134.1"
                     /translation="MNKFTGYRCSLCGKEFDASYSGYVCDADQGNLNVILNYDQIRNS
                     IDPKQIFQSAEPSLWRYAPLLPVEDPGFFGTPLHSAGWTPVYDEPRLAAKIGVRNLWI
                     KDDGRNPTASFKDRASAVLIARAKEINAKTIVTASTGNAGAALAGMAAASGWHAIIFA
                     PKSAPPAKIAQLLVFGAEVILVDGNYDSAFDLSVSAAERYGWYCRNTGYNAFTAEGKK
                     TAAFEIWEQIISKQNTSSAPLNVFVSVGDGNIISGIHKGFSDLKELGWLPQMPRIFGV
                     QSEKSSAVYQAWAKGSEEIIPVHATTFADSISVDLPRDGVRAVRAATKTGGAFIAVSD
                     ESIYRGISELGKIGVFAEPAGAAAYVGMEKAAEEGLIGPDDDVVVINTGSGLKDIRSA
                     MKATGEAVIIEPSLAALEKYLKAKGN"
     gene            7855..8829
                     /locus_tag="PK898_08630"
     CDS             7855..8829
                     /locus_tag="PK898_08630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU20135.1"
                     /translation="MKPTYSVIVPVYNEKESLPVLYERMVSVMDQTGEPWELILVDDG
                     SFDGSSQIMRELSQKDPRVRVIFFARNFGHQIAVTAGMDHALGDAAIVIDADLQDPPE
                     VILELIEKWKEGYEVVYAVRTGREGESWFKKETASVFYRLIYKITDVKIPLDAGDFRL
                     LDRKVLDVMNSMREKHRFLRGMSAWVGYKQIGVPYQRAPRYAGKTKYPLKKMILLAWN
                     AITGFSYVPLQIAMILGFIVAFISILLIIVVIILRINGNELVGQATTLISTLFLGGVQ
                     LICLGIIGEYLGRIYDEVKNRPLYIAAEITPSIYDEDSEKPSEQERKE"
     gene            8953..10419
                     /locus_tag="PK898_08635"
     CDS             8953..10419
                     /locus_tag="PK898_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU20136.1"
                     /translation="MARIDLTIKKDRLEHAVERARQRNIIIPTFEEQIHPELVPEKIK
                     KELKSIGLWDIHPRNLYRITWKNEPVEKGGTFGDVNYIELPSSLTGVDARIVALVGKW
                     FPTGAHKVGAAFACLVPRLVTGQFDPTEEKAVWPSTGNYCRGGAYDSALLACKSIAIL
                     PEGMSRERFAWLEKVADEVIATPGTESNVKEIFDKCWELRKSGEKLMIFNQFEELGNY
                     LWHYHVTGNAMREVWEKLMRKNDRLAGICSNTGSGGTIACGDYLKTLHPNMKIVASEA
                     LQCPTLLMNGFGAHRIEGIGDKHVPWIHNVRNTDFVTSIDDNACVHLLRLFNEPEGKK
                     YLVEKGVPKEIVEQLNLLGISSVANLLSAIKFAKYNELTGEDMVMTVFTDSVDMYTTR
                     LAEYNEEFGPFTRDDASYAFGRYLMGENTSNMLELSYYDRKRIHNLKYYTWIEQQGKT
                     SEELNAQWYDRNYWTEIAAQKDEIDTLIKSFNEQVGLL"
BASE COUNT         2952 a         2290 c         2527 g         2789 t
ORIGIN      
        1 aatcgataaa agcagaattc ttttttatac accgaaaagg taaaaacatc gtccacagct
       61 taaaggattt cgcctgctat acttaaatca tgattgaaat aagcccgaat gttaagatta
      121 atgaaaatga gattcaattt aattttattc gcggatcggg ccccggcgga caaaacataa
      181 acaaagtcgc atcatcagtt cagttacgat ttgatgtgcg caattcgact tcattggatc
      241 cagagatcaa acagcgcttg atcaaacttg ccggtaatcg catcaatgat cagggagtcc
      301 taatcatcga tgccaaacga ttccgtactc aggaacaaaa tcgttttgat gccactctgc
      361 gactgatcga tttaatccgg aaagcatcga tcacacccaa aaagcgaaaa gcaacccatc
      421 cgagcgttac ggcaaaggca gcgcgcagag gtgagaagaa aaaacatagt gaaacaaaac
      481 gccttcggag atatataccg gaagattggg agtaatcttt cggaaaagtt ctaagaacga
      541 ttatcaataa tatataaggc ataatttgat cagaacatat tccgtatatg atcctttgat
      601 tcaaaagaat aaagggcttc gtttaataga cgttattgct gaatcacaac acaccctcat
      661 ttcgttgcct tgacaaaaag taccaaaggt ttaaaataag taagcattaa tgccgaagtg
      721 gcggaattgg cagacgcgct acgttcaggg cgtagtgaat gtactttcat gagggttcaa
      781 atccctcctt cggcaccaga tgatacagaa aaagaatccc gttggggatt ttttcttttc
      841 aaagggaggc catttatccg gcaaaaaagc ttggtcgaaa tcattcgctg gtaaacttaa
      901 agcatgaaaa aatggcttct cacaaaaaag caaatctggt tggttatttt ggttttggtc
      961 gtcgtcagca tgttgatcga cctgaatacc cgtatcagca cattgactta tttgcgagat
     1021 cagaaaaata ccttggcagt ggaagtaacc gctttacaat ccacgctgga tcttgtatct
     1081 gaaaaaatcg attatgccgg ttctgatgct gcagttgaat cttgggcaag acaacagggg
     1141 ttgatgagac aggaagggga ttacgtcatt attccgcttc cggtggatga acccacaccg
     1201 attcccacac cggttccgac cgtggaaccg gtcaaatatg aaaattggga aatctggaaa
     1261 gctttgattt ttgactaaat aaaaaaggaa cagatgagcc cgatccatac ggctcggcta
     1321 aactatgacc gctgtcatca ttgatggaaa gaaaatagca gaaggaatcc gaaacgaggt
     1381 cggcattgcc gtaaaagaga gaatcgctgc cggtttgtcg gtcccggggt tagcgaccgt
     1441 gttggttggc aatgatccgg cttcccaaat ctatgtccga aataaaatga aggcttgtga
     1501 ccggttggga atttattcgg tttcttatac gcttccggaa gataccgatt ttgaaacgct
     1561 caaagagttg atcaatcaat taaatgtcgc gcccgaaatt catggcatcc ttgtccaact
     1621 cccgttaccg ccgcaaatcg atgaatattc tgttttatcg atgatcaatc cccaaaaaga
     1681 tgtggacgga tttcatccga ttaacgccgg catgctggcc cggaaagaaa gtgagccttg
     1741 gtttatcccg tgcacttcgg cgggtattct gcgtttaatt cacagtgttc aaccggacat
     1801 caccggtatg gaagccgtag taatcggccg atcttctatc gtcggtatgc cgaccgcgca
     1861 gttgctgtgc cgaaataacg ccacggtcac catctgtcac acaaagaccg tcaatctcgc
     1921 tgccgtttgc cgccgtgcgg atctgttggt agtcgctgcc ggacacagca aactggtaaa
     1981 aggggattgg gtaaaaccgg gcgcaatcgt gattgatgtg ggaatcaatc gagtgaatac
     2041 gcccgactca cccaaaggat atatcctgac aggtgatacc gaatttgatg agattgtcaa
     2101 cattgccaaa gcgatcacgc cggttccggg cggagtcggt ccgatgacga tagcgatgct
     2161 tatgcaaaat accctgctcg ctgcttccag atattccgct taacattttc cttcttcaaa
     2221 tttcatttcg atttgtcgta tattatgatg aatatcatat tgtcagacaa tccaaaatgg
     2281 ggtataaaca agggagtaga actgctctaa atgattagac aattctttgc ttatcaaaaa
     2341 ggaggaaaga atcattgtcc acacttttaa ttaaaaatgc gttggtactg gctaccatgg
     2401 atgacaatag acaggaaatc tccggcgggg gattattgat tcgggacgga tttatcgaaa
     2461 gcgtcgggaa aacatcggat ttgccggcgg aagcggatga aattctggat ttatccggct
     2521 gtgtcgttct tccggggctg attaatacac atcatcactt ttatcagacg ttgaccaggg
     2581 caattcccgg cgctcagaat tcgaatctat ttaattggtt aaaaaccctt tatccgattt
     2641 ggggcagaat gactccggaa gatatctata tttcgacaca gacagccatg gcagaattgg
     2701 cgctttcagg ctgtacaacc gcttccgatc atttatacct tttcccgaac gggtctaaat
     2761 tggatgatga gatcgaaggt gctcgccaag tgggattgcg tttccatgca tcgcggggat
     2821 cgatgagcct cggcgaatcc aaaggcggac ttccgccgga cagcgtcgtt gacagcgaag
     2881 aacatatttt ggaagattgc gttcgggttg tcgaaaaata tcatgatccg aaaccgggat
     2941 cgatgctgca aattgttttt gcaccttgtt ctcctttcag tgtctcaacc gaattaatgc
     3001 gggaaagcgc aaaattagcc cgtcactacg gagtccattt gcacacccat ttggcggaga
     3061 cagaggatga ggaagctttt acactcgaaa aattcggcct cagaccggta gattatatgg
     3121 cttcggtcga ttggctcgga aatgacgtct ggtttgcaca ttccgtccat gtcagtccgg
     3181 aagatataca agtttataag aaatacggtt gcggagtcgc tcactgtccg tcatcgaata
     3241 tgcgtctcgg ctccggtttc gctccgttga tggaatattt acgggaagga attcacgtcg
     3301 ggttgggagt ggacgggtcc gccagtaatg acggctccca tatgttggcg gaagttcgac
     3361 aggctctcct gatggcacgc cttcgtgccg gtttgatggg cgcttcgctc agcggtgaaa
     3421 atgcgcccaa attaatcacc gctcgggaag tgctggaaat tgcgacgcgc ggcggagcgg
     3481 cggttctggg cagaaaagat atcggctctc tggaaccggg aaaatgcggc gattttatcg
     3541 ccgtcaatat gaatcgatta cgttataccg gcagcttgac cgatccgctg tctgcattgg
     3601 tattctgcaa cccgggctgg gttgatttca attatgtcca aggaaaagca atcgttaaaa
     3661 acggggaatt aatgaccgtg aatgtgccgg atctggtaca acgacataat aaagccgctg
     3721 ccggattggt gagaaattat taaatcaggt gattagatga aaaaccaaga aaacaattat
     3781 gagaatctca gcctttttca gaaactgaat aatgacttag caaaaattat tgaagagaca
     3841 cctcccggta ccagactgaa atccgagccg gaattggcgg caagtttagg cgtgtctcgc
     3901 gcgacgttgc gcgaagcgat gcgaacgttt gaagggcagg ggctgatccg tcgccgacag
     3961 ggagtcggaa cttttgtcct cgatcaaaag caaaacatcg aaagcgggct ggaagtgctt
     4021 gaaagcatcg aaacacaggc aaaacggctg gggttggatg tgaagatggg atatctggaa
     4081 atcaaccaca tcaaaaccga tgaagaactc gccaaacact tcggcatcca acagggtgat
     4141 cccgttatcc gaatttcaag ggtaatctgg gtcgctgacc gtccggtggc atatttaatc
     4201 gataatgtat tgccggatgt tttggaagag gaagatcttg ataaagggtt caccggctcg
     4261 gtgttggatt tattcctgag acgagggaag ccggaactct cacactccaa aacattggtt
     4321 gccgctttcc ccgccacaac cgctatcgcg aaaaaaatgc agctccaaag aggagattgt
     4381 ctcctcaggt tggaagctta tctttatgac aaaaaagatc gaatgattga ttattctctc
     4441 agctatttca ttccgggatt tttcaaattc caagtgattc gatcaatcgg gaaataaatt
     4501 tatgaccaat aagtcatcct ccggaagcaa ccggccttct ttaccggtcc tgtttacctt
     4561 ggcgattctg atcgcttatg gggttccggt atttttaggc tggttcagtt tctatgggga
     4621 cgactggatt tatatctatg aatatcatct gaacggtacc gccggatttc ccgcttttgt
     4681 cgcttgggac cggccgtatt ccgcatggat ctatattctg gcaacttctc ttttcggaga
     4741 acatgttttc ccgtatcacc ttttattgct tcttcagcgc tggttggctg tctttttgtt
     4801 tttcaaaatc ttgcaaatgg tttggtcgga tattccgcgt gttgtcagcg gagccgtatt
     4861 actctttgcc gtttatcccg gctttcacca acaaccgatc gcagtccaat atattttgca
     4921 tttcgcatca ttggatttat gcttgttttc catttggtgc atgctgaaag cttttgattc
     4981 cggttttttg gagaaacaat catggaaagg tatcctgctc acaatcgccg gattcttttc
     5041 ttcgttaacg gcactttttt cctgtgaata ttttatcggg ctggaattgg ttcggccgtt
     5101 cctgttatgg ttccgacaag tcaatcgatt taaagacgaa ccggttaaaa agccggtgaa
     5161 aatcggaaat atcttttttc actggttccc atatctgttg tccatgatga tttttatcgt
     5221 ttggcgggtt tttattttct cattccagac ctatcagccg aagcttctta acaaattcaa
     5281 cgaaaacccc gctgccgcat tatcggagat tctccgaaaa attctgaccg atctttatac
     5341 ggttacgata gcggcatgga aaaaatcctt gaaccaacct gccggaaaag agaatcaggt
     5401 catcttctgg ttgttgatat tagtctgttt tttcctcttt gcttattctc tcagacggtt
     5461 tcacaaccga gaagggaata aacaaccggc ggcgatcgaa aatgagaaga ttcaattttt
     5521 gatcaccgga ctggtcgctt tgttggcagc cggtattccg tattgggtga caatgatcaa
     5581 tatcgaactc gatttccctt gggaccggcc gaccatttct ttcagtatcg gtgtcgctat
     5641 gttgatcagc gtcggaattt cgtttatttt ccaaaacaaa tttcaaacct tggtcaccgc
     5701 gtcgctgatc gcattcgcta tcggctccca ttacaccaac gccctggtct atcgtaatga
     5761 agccgaaaag atgaacgatt atttttggca acttgcttgg cgagcgccgc aattacaacc
     5821 ggggacaatt ttggtttccg aacagattcc gctcgatcgc tacagtgata atgatctgac
     5881 accgattgtc aattggcaat atgcacctgc tttaaaaggg aatacttatc aatataaata
     5941 ttttgatttg gatttgcgca gcgagactta ttttcgggat ctccgacccg gtcagccgat
     6001 tgtgcatgat tatcgcagtc atcattttga atcggacagc agtcacgttc tggcattatt
     6061 ttacaaaaaa ggttcctgcc tttggacgat taccgaatcg gaagccgaat atcccgggtt
     6121 accggaaaat attgttcatg tggccggaat ttccgatatc aatttgattc ggtccgattc
     6181 ggccgttcaa gccgtgccgc cgtctccgat cggaaaagaa ccggatcacg gatattgcta
     6241 ttattttcag gaaatcaacc gcgcttttca gaatcatgac agagaaacgg cttatcaagc
     6301 cgcggatacc gcgttaaaaa tgggattatc ctcgcaggat cctttggatt ttatcccggc
     6361 ggtgaaagca ctaatcttag gcgaagattg gtcccgagcc gcggagttaa gtaaaaaagt
     6421 catcatgaac ccggagaatc tgcctttttt gtgcgagcaa atatccggga ttcccgaaac
     6481 agtcaatcaa ccggaatata ccgaaatgct gaaaacaata ggttgtgtaa ctcaataaaa
     6541 aatcaaaccg acaaggaaac aggacgaaga attatgaata aatttacagg atatcgatgc
     6601 agtctctgcg gaaaggaatt tgatgcctct tattcgggct atgtctgtga tgcggatcag
     6661 ggaaatttga acgtgatcct taattacgat caaatccgaa actcgatcga tccgaaacaa
     6721 atctttcaat ccgccgaacc ttctctgtgg cgttacgcac cgttgttgcc cgtcgaggat
     6781 cccggatttt tcgggacccc gcttcattct gccggatgga ctccggttta tgatgagccc
     6841 cggttggcag caaaaatcgg cgtccgtaac ctttggatca aagatgatgg acgaaatccg
     6901 accgcttctt ttaaagatcg agccagcgcc gttctgattg cccgagcgaa agaaataaat
     6961 gccaaaacca ttgtcaccgc ttccacggga aatgcgggag cggctttggc agggatggct
     7021 gctgccagcg gatggcatgc cattattttt gcccccaaat cggcaccgcc ggcaaaaatc
     7081 gcacagcttc tggttttcgg cgccgaggtg attttggtag atggaaatta tgacagcgct
     7141 tttgaccttt ccgtatccgc cgcagaacga tacggttggt attgccgtaa cacaggttac
     7201 aatgctttta ccgcagaagg caaaaaaacc gccgcttttg aaatttggga acaaattatt
     7261 tctaaacaaa acacatcatc cgccccatta aatgtctttg tttccgtcgg agacggaaat
     7321 attatttccg ggattcataa aggattcagc gatcttaaag agctggggtg gcttcctcag
     7381 atgccacgca ttttcggggt ccagtcagag aaatcatccg ccgtttatca ggcatgggca
     7441 aaaggcagcg aagagattat cccggttcat gcgacgacat ttgccgattc catctccgtg
     7501 gatctgcctc gggacggcgt gcgcgcggtt cgagcggcaa cgaaaaccgg cggagctttc
     7561 attgcggttt cggatgaaag tatttaccgt gggatctccg aactcggtaa aatcggggtt
     7621 ttcgccgaac cggccggggc tgcagcttat gtcggaatgg aaaaagcagc ggaagaagga
     7681 ttgatcggac cggatgacga tgtggtggta ataaataccg gaagcggatt gaaagacatt
     7741 cgctccgcca tgaaagcaac cggggaagcg gtgattatcg agcccagtct tgccgctttg
     7801 gaaaaatatc tgaaagcgaa agggaattag cccttccaaa aaaaggattg caatatgaaa
     7861 cctacttatt ctgtgatcgt cccggtttac aacgaaaagg aaagcctccc ggttttatat
     7921 gaacggatgg tttccgtaat ggatcaaacc ggcgaacctt gggaattgat actggtggat
     7981 gacggatcct ttgacggatc atcccaaatc atgcgggaac tttctcaaaa agaccctcgt
     8041 gtccgggtta tcttttttgc ccgtaatttc ggccatcaga ttgcggtgac tgccggtatg
     8101 gatcacgctt tgggcgacgc ggcaattgta atcgatgccg atcttcagga tccgcctgag
     8161 gtaatcttgg aattaatcga aaaatggaag gaagggtacg aagttgttta tgccgtaaga
     8221 acaggaagag aaggggaatc gtggtttaag aaagagacag cttccgtttt ctatcgctta
     8281 atttataaaa taaccgatgt gaaaattccg ttggacgccg gcgatttccg tctgttagat
     8341 cgcaaagtct tggacgtgat gaattccatg cgagagaaac atcgattcct acggggaatg
     8401 tcggcatggg taggttataa gcaaatcggt gttccgtatc aacgagctcc acgctatgcg
     8461 ggaaagacca aatatccgtt gaaaaaaatg atcctgctgg cttggaacgc aattacgggt
     8521 ttttcttacg tcccgctgca aattgcgatg attctcggct tcatcgttgc attcatcagt
     8581 atcttactga tcatcgttgt tattattctg cgcatcaacg gcaatgaatt agtcggacag
     8641 gcaacgacat taatctcaac tttattcctc ggcggcgttc agttgatttg tttgggaatc
     8701 attggcgaat atttgggacg gatctatgat gaagtaaaaa atcgacctct ctatattgct
     8761 gcagaaatta ctccctccat ctatgacgaa gactcggaaa agccatccga acaggaaaga
     8821 aaagaataaa tttacgggag gaatcattta ttcgatacag aatatcagaa tgtcaaaaag
     8881 attatataag ttttagttat ctaaaacgag acctgcaaac aaaacaaact attaagaaat
     8941 aagaggaaaa aaatggcacg aattgattta acaatcaaaa aagatcgctt ggagcacgct
     9001 gttgaacgcg ctcggcaaag aaatattatc atcccaacct ttgaagaaca gattcatccg
     9061 gaattagttc cggagaaaat caaaaaggag ttaaagtcta tcggtttatg ggatattcat
     9121 ccgcgaaacc tttatcgaat cacctggaaa aatgaaccgg ttgaaaaagg cggaaccttc
     9181 ggtgatgtca attatataga attgccttcc agtttgaccg gcgtcgatgc cagaattgta
     9241 gctttggtgg ggaaatggtt cccgaccggt gcccataaag tcggtgcggc gtttgcctgt
     9301 ctggttccca gactggtaac tggacaattt gacccgacgg aagagaaagc ggtttggccc
     9361 tccaccggga attattgtcg cggtggagct tatgattccg cattactggc atgcaaatcc
     9421 atcgcgattc ttccggaagg aatgagccga gaacgcttcg cctggctgga aaaagtcgcc
     9481 gatgaagtga tcgccacccc cggaactgaa tcaaatgtga aagaaatctt tgataaatgc
     9541 tgggaattaa gaaaatcggg cgaaaaattg atgattttta atcagttcga ggaactgggt
     9601 aactatcttt ggcattacca tgtaaccgga aatgccatgc gggaagtttg ggaaaaatta
     9661 atgaggaaaa acgaccgact ggccggaata tgctccaata cgggttccgg cggcacaatc
     9721 gcctgcggag attacctcaa gacactccac ccgaatatga aaattgtcgc cagcgaagct
     9781 ttgcaatgcc cgactttatt aatgaacggt ttcggcgctc atcggattga aggaatcggc
     9841 gataagcatg ttccttggat ccataatgtc cgcaatacag acttcgtaac ttcaatcgat
     9901 gataatgctt gtgttcattt gctgaggctc ttcaatgaac cggaaggaaa aaagtacttg
     9961 gttgaaaaag gcgttccgaa agaaatcgtc gaacaactca acctgttggg aatctccagc
    10021 gtagccaatt tgctgtcggc gattaagttt gccaaataca atgaactgac cggcgaagat
    10081 atggtaatga ccgttttcac cgattcggtc gacatgtata caacccggtt ggccgaatac
    10141 aacgaagagt tcggaccatt tacccgtgat gatgcctctt acgcattcgg tcgctatttg
    10201 atgggtgaaa atacatcgaa tatgctggaa ttgtcctatt atgatcggaa acggattcac
    10261 aatttgaaat attacacatg gattgaacag cagggcaaga cgtccgaaga attgaatgcc
    10321 cagtggtatg accgcaatta ttggacggaa atcgccgccc aaaaagatga aatcgacact
    10381 ttgattaaat ccttcaatga acaggttggc ttactgtaaa tcagaggaat gcggcattta
    10441 tattccatag ccgtacattc agaaagtccc gcatcgatcg atgcgggact tttgcatctc
    10501 aataaaagct atgaaatcat atctaaaaaa tgctgatgaa ccgtcggatc atccgtta
//
LOCUS       DAOSMM010000067        10479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_6346_length_10479_cov_18.223523, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000067 DAOSMM010000000
VERSION     DAOSMM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6346_length_10479_cov_18.223523"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PK898_08640"
     CDS             <1..683
                     /locus_tag="PK898_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU20137.1"
                     /translation="AMVKGLIFDFNGTLYFDTDKQEEAWRIYIQKKYHRSINPTEFRD
                     HIHGRAFDSIFEYYLGSDSSEKEQKEMIEEKEAIYREIARADQKNLHLVSGAESLFDE
                     LSGRKIPFTVATASGIKNVQFYFEVFPLYKWFVGPEDWVYDDGTIPGKPEPDIYLKAA
                     KKIGIEPANCLVVEDSFPGITAAIDAGIGKVIAMNPAGDRDKIEKMNDVKAIISDFNG
                     FAERFLNE"
     gene            676..1746
                     /gene="mazG"
                     /locus_tag="PK898_08645"
     CDS             676..1746
                     /gene="mazG"
                     /locus_tag="PK898_08645"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU20138.1"
                     /translation="MNEQKEIMNCQDNLIILEGKTLLSLRHLLVPPHLEVIIRNPRNA
                     PHLQDILSEEYPADHPLEIYMDVPENQQNSLTIKLAELSQQDHYLEKALWIRIPPLPE
                     SRSFEYFQNIVAILRAPNGCPWDRKQTHQSLRDDFLQEAYELLEGLDRNDVEMISEEL
                     GDVLLHIVMQAQIAQENNEFNMGDVLAKISEKLIFRHEHVFGNPEKLSSDQVLNRWEK
                     VKNLEREKDHKEQGLLDGISKAMPALSMAYSYQKRAARVGFDWESIDGVWDKLYEEIE
                     EFKRADTPEDQAEELGDILFTLVNLARWSKIDPETALRNSNLKFHDRVQYVETKAHEL
                     GKNLFDMPMSEKDQYWDEYKES"
     gene            1727..2419
                     /locus_tag="PK898_08650"
     CDS             1727..2419
                     /locus_tag="PK898_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20139.1"
                     /translation="MSTKNPEDQAKKAFPVWFLIALLFTLVWFTGFLWLKQANKEVTE
                     THKTRAVTIPIELQDKSCVLTIPACELELRTPEKISLANRANIDLKINPSENLYWDCN
                     SEIPKNLRVQLFARIEFPAAKIIPADQMGQEFPAGHEASFHWTIIPNQSGKNIQGQLW
                     LKAEIIAVQTDSNQKLEEWSLFSNEVKMTVIDIGKIPTDLLKRFLDGILLFGCGSWVI
                     FILQKTFRKRRK"
     gene            2517..2819
                     /locus_tag="PK898_08655"
     CDS             2517..2819
                     /locus_tag="PK898_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOU20140.1"
                     /translation="MEKEIKKPKNIFGSDSRLLKKAFSLFLIIGIITLIIAFLSLWGG
                     RKLDSLYGTSPWIMIGLGIGGFLLVALIIVKMTKRVLRELNNSIKTSNKTGEDQND"
     gene            2812..3831
                     /gene="atpB"
                     /locus_tag="PK898_08660"
     CDS             2812..3831
                     /gene="atpB"
                     /locus_tag="PK898_08660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOU20141.1"
                     /translation="MTETVQTEIRPVEKKKGSKLWLISLLLLVVLNFVVVKFYKPVEP
                     EINIATDSLLFHMEDGVAVRDPWFTIPFIGDVYLTNTMPAIALVFILLLLLAAAVHQQ
                     TKDGQLKSHGVVLLLEIIFSALTGMTDSAVEEKWRDRIYPFFLTVFMYVLIANLTKLL
                     PFFETFGLVYPTNENGFIAQKWGSWFYAIQSTAAEAGQQGYRLVSLFRGASTDLNFTV
                     AIAIVTVILIQVIGVKAHGFSYFHKFIDIKGMITNPKKGLINGFVGILELISEIAKIA
                     SFGFRLFGNMFAGMVLLLFLGYMVPWLVNTVILLYEAFVGILQAFIFGMLATVFMSMA
                     VRSES"
     gene            3855..4079
                     /gene="atpE"
                     /locus_tag="PK898_08665"
     CDS             3855..4079
                     /gene="atpE"
                     /locus_tag="PK898_08665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOU20142.1"
                     /translation="MDPQIGKYIGAGLVMISTIGAGIAIGNIGKGALDGISRNPDAYG
                     NLMTTMILAIAFAESLAIYSLVVAFLILFG"
     gene            4095..>4717
                     /gene="atpF"
                     /locus_tag="PK898_08670"
     CDS             4095..>4717
                     /gene="atpF"
                     /locus_tag="PK898_08670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOU20143.1"
                     /translation="MEALGINAGNLFVNIICFAIAYLIIAKLIVKPIKKMTDNRKEVI
                     DKGIADAKIAADLRENALQESEKILNAAREESARMLKEATDQIAQMKEDYRKSVDAEV
                     SKELRDSREYLNKERELMLSNLRGQIIDLSISGAKKILSEELSMDEAKQHKLLNDLFT
                     GIKDGSIPGIDRLPNNLTKMEVTTAVPLTDEERAIVEAQLSGKLAEGV"
     assembly_gap    4718..4817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4818..6013
                     /locus_tag="PK898_08675"
     CDS             <4818..6013
                     /locus_tag="PK898_08675"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOU20144.1"
                     /translation="GVYNKTMPVNYLQLQSQIHILSDSILNQREKGKEALEKLTDQFK
                     AAANLPDLAERVSAKLRENRGLSCALPREERIDSHISASDHSGEIVNILAADGSQINP
                     SRHEEVSFGLINTAIFKMRMNGNEAPQIVTETKLLEREDDSLQEKLNNEANVGLFRDV
                     EERRLLSREACKIPQGEPVVALTDGPLQLYYFQEPNTTENPLVNEYYKALAEMSEKKI
                     ITAGYTDRPRSTFVIDMLRLLMNSDPQDLRVKQTLVFDSNLFSRILLPGERSALFKLI
                     SPAVEQMPAVVQISFFYLNVGNDSRPMITRIEIPEWVSKDPAAIDLLHYSLLQQNGFI
                     SSNPYPYALHRAHECAVVQFSEKEHVKQLIIRELLSRGVTDLGKSAKQIAKDLFGTTY
                     RDFEE"
     gene            6052..6693
                     /locus_tag="PK898_08680"
     CDS             6052..6693
                     /locus_tag="PK898_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20145.1"
                     /translation="MTDTSMFGRILSSNIRECVVGYRTSESAIPPFGSMVRIPVSHAQ
                     QPEIYGLVTDIQIEEDGFLRQLATNDQVSEEVMLDARANRNVPVVLRILFIGYLENGN
                     ISHLLPPCPPLTLDNIYKCTNAEITAFTSFGNFGYIRHILKVTNVPPGELLAAHISQA
                     AKAQFADNHFGWTERAINEVIYLMRDNYADLLAILGAIGDADISLDGEEEEQE"
     gene            6690..8348
                     /locus_tag="PK898_08685"
     CDS             6690..8348
                     /locus_tag="PK898_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU20146.1"
                     /translation="MTPAENIGFVIGGSLKEGLFVRLTVPAERIQEGAFVVIESNGLK
                     FYGLVVNLSLKSYDPRYSQDLIAGRMSDKITTLLHNQTLFTEMEVLPVLMQNTGPELE
                     DPNYDPNAVMDKSPMPVKTLPIHQAPVRLATEFDVSEVFKPKEKNGFVIGTTREQGHP
                     VHIDMDKFIQRSSGIFGATGTGKSYLTRMILAGLIQSKQAAVLVFDMHNEYAYGDISP
                     DTDIKAPGLCEKMKKRVKSCALGASAKIAGNQPDFDLVLEYSDILPSDIEMLTRELNL
                     RETTPATLSALVQSFGINKWFESFKNLTMGEGEGSVVDWARNNNVNEAAALSLYSKLE
                     RVFRLNYLVPKLTGPSSIVKIIDYLKNGYSVILSFGEYETDLDYLLVTNILTRKIREE
                     WERMTNEYRNQGGVKPTPLVIALEEAHKLLNREMAGQTTFSTIAREMRKYFVTLLIID
                     QRPSQIYDEVMSQLGTRISGWLGDDEDIAAVLSGLSGRDSLRGMLARLQPKEEVLLLG
                     YGVPMPIPIRSRRYDAQFWQEMLNEDELSATDKQKFREMIFDDF"
     gene            8379..>10479
                     /locus_tag="PK898_08690"
     CDS             8379..>10479
                     /locus_tag="PK898_08690"
                     /inference="COORDINATES: protein motif:HMM:NF039324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HOU20147.1"
                     /translation="MTEAGPNRSIERQKEPKILVISLFLAIQFALCIYLRWIPYPIHS
                     KRALAAMILLIGLFTICTDFIISRWILIRFRRTTKTVFCFTLLASLIFGGLLLAVQTV
                     PVPDRYFFLPDGSVKITAIAEKNPLSNGKKVEILYFDTGTTDNINAFEQAGNWSVQNK
                     TVVTEEEGSLYWNGKVFHRIQLLFASGPDCGIVRIDWAGKSQRIDLYAEQAGEILFTQ
                     DFPFSWINRAVIISAYWIGISFLFLLFLTLTVPVKSQKKFAEAEAIPAWWVFALPMIF
                     VWTICLLTFWPGILTPDSVVQWGQAVAKVPLDNRNPAIYTLLIRLLTTVSNTPAIVAF
                     AQILALSLVTAGGIRFLLNQHISVKTAWILSIIMAFSPVNLFFPITIWKDVPYGISML
                     AVFLILLQIVISDGVWLKKRSNRIWLILSGICLSLFRHNGFPVLVATLVIFLLFYRQI
                     RGLLFEALVVIIAAYGLVLGPVYSVFHVTKGNDNGINPILVHYISAHIEAETPLSESE
                     RTWLNQVYPLDQWKYDPCVINNLWFLPGFNEKVLQENPKEVMNIFLSLSKKAPAVSLR
                     HAFQSSNMIWKIQHGGCYLYRIGLLGQSDGTLKWVEANQIGVVQESKLPKLTDLLFNF
                     FLKTAGPTFPDAIFWRPAIYTYWTLFVSTILALRFSKPKYLLIGLLTLVQSGMMLFIT
                     IAQDMRYQYGVMLIGIFC"
BASE COUNT         2976 a         1969 c         2438 g         2996 t
ORIGIN      
        1 aagctatggt gaaaggtctg atttttgatt tcaacgggac gctctatttt gataccgaca
       61 aacaagaaga agcttggcga atttacattc aaaagaaata tcatcgatcg atcaacccaa
      121 ccgaatttcg cgatcatatc catggcagag catttgactc tattttcgaa tactatttag
      181 gttccgacag ttccgaaaag gaacaaaaag aaatgataga agaaaaagaa gcgatctatc
      241 gggaaattgc acgggcagat caaaaaaatc tacatttggt ttcgggggcc gaatcattat
      301 tcgacgagtt gagtggacga aaaattcctt ttaccgttgc aacggcatcc ggaattaaga
      361 acgttcaatt ttattttgaa gttttccctc tttataagtg gtttgttggg cctgaagatt
      421 gggtttatga tgacggaacg attcccggaa aacccgaacc ggatatttat ctgaaagcag
      481 cgaagaaaat aggaatcgaa cctgcaaatt gtctggtggt ggaagattct ttcccgggga
      541 taaccgccgc aatcgatgcg gggatcggaa aggtaatcgc catgaatccg gctggagata
      601 gggataaaat tgaaaagatg aatgatgtga aagcaattat ttcagatttc aatggatttg
      661 cggagaggtt tctgaatgaa tgaacaaaaa gaaattatga attgtcagga taatttgatt
      721 attttagagg ggaaaaccct tctttcgtta cggcatttat tggtaccccc tcatttggaa
      781 gttatcattc ggaatccgcg aaacgcaccc catttgcaag atatcttatc ggaagaatat
      841 cccgcagatc atcctcttga aatttatatg gatgtgccgg agaatcagca gaactcgtta
      901 acgataaaat tagcggaatt gtcacaacaa gatcattatt tggaaaaagc actgtggata
      961 cgaattccac cccttccgga gagtcgatcc tttgaatatt tccagaatat cgtcgccatc
     1021 ttaagagctc ccaacgggtg cccgtgggac cgcaaacaga ctcatcaatc cttacgggat
     1081 gattttcttc aggaagctta tgagcttttg gaaggattag atcgaaatga cgttgaaatg
     1141 atttccgaag agttggggga tgttctgttg catattgtga tgcaggcgca aattgcacag
     1201 gaaaataatg agtttaatat gggggatgtg ctggccaaaa tcagtgagaa attgattttt
     1261 aggcatgaac atgtttttgg aaatccagaa aagttatcat ccgatcaagt tttaaatcga
     1321 tgggagaagg ttaaaaactt ggaacgtgag aaagatcata aagaacaagg tctgttagat
     1381 gggatttcaa aagcgatgcc ggctttatcc atggcttatt cttatcaaaa acgtgccgcc
     1441 cgagtcggtt ttgattggga atcgattgac ggcgtttggg ataaacttta tgaggaaatc
     1501 gaagaattta aacgggcgga cactcctgag gatcaggctg aagaactggg ggatatcctt
     1561 ttcaccttgg tgaatctggc acgttggtca aaaatcgatc cggaaactgc cctgcggaat
     1621 tccaatttaa aatttcatga tcgtgttcaa tatgtggaaa caaaagcgca tgagctcggg
     1681 aaaaatctct tcgatatgcc gatgagtgaa aaagatcaat actgggatga gtacaaagaa
     1741 tcctgaggac caggcgaaaa aggcttttcc ggtttggttt ctgattgcgc tgctgtttac
     1801 cctcgtatgg tttacgggtt ttctatggtt gaagcaagcg aataaagaag taacggagac
     1861 tcataaaacc cgcgctgtta cgataccgat tgaattacaa gataaatcct gtgttcttac
     1921 aattcctgcg tgtgaacttg agctccgaac ccccgaaaaa atttccctcg ctaatcgagc
     1981 gaatattgat ctgaaaatca atccttctga aaatttgtat tgggattgta attccgaaat
     2041 tcctaaaaat ctcagagttc aattattcgc acggattgaa tttccggctg ccaaaatcat
     2101 tcccgcagat caaatgggac aggaatttcc ggccggtcat gaggcgtctt ttcattggac
     2161 aatcattccc aaccaatccg gaaaaaatat tcaagggcag ctttggctga aagccgaaat
     2221 tatagcggta caaaccgatt caaatcaaaa actggaagag tggagtttgt tttctaacga
     2281 agtaaaaatg acggtgattg atatcgggaa aattccgacc gatctcttga agagatttct
     2341 ggatggaatt ctactattcg gttgcgggtc atgggtgata tttatcttgc aaaaaacttt
     2401 tcgaaaaaga agaaaataag tacgttgtac caattctttc tgtgacgtaa atcacgaaat
     2461 cgcatggctg atataataac gattgattta ggagtaagaa aaggtgcggt atgagcatgg
     2521 agaaggaaat aaaaaaacca aagaatattt ttggcagtga ttcacggctt ctcaaaaaag
     2581 cattctcatt attcctaatt atcggaatta ttactctaat catcgctttt ttaagccttt
     2641 ggggtggaag aaaactggat tctttatatg ggacgagccc ttggatcatg atcgggcttg
     2701 gaatcggtgg ttttttattg gttgctttga tcattgtaaa aatgacaaaa agagttttgc
     2761 gggaattgaa taactctatt aaaacttcga ataagacggg ggaagatcaa aatgactgag
     2821 acggttcaaa ctgagattcg tccggttgaa aagaagaagg gatcaaaact ttggcttatt
     2881 tcccttttgc tgttggttgt tctgaatttt gttgttgtca aattctataa acccgttgag
     2941 ccggaaatta acatagcgac ggacagctta ttatttcata tggaagatgg cgtagcggtc
     3001 cgagatcctt ggtttacgat tcctttcatc ggggatgtct atttgacaaa cacaatgccg
     3061 gcaattgctc tggttttcat tctattactt ctgttggcgg cagcggttca tcaacagacg
     3121 aaagacggac aattaaaatc tcatggcgtg gtgttgcttc ttgaaatcat tttttcggcg
     3181 ttgacgggga tgacggatag cgctgtagaa gaaaagtggc gagatcgaat ttatcctttt
     3241 ttcctgaccg tatttatgta tgtattaatc gcaaacttga ccaaattatt gccgtttttc
     3301 gaaacattcg gattggtcta tccgacgaac gaaaacggtt ttatcgcaca gaaatgggga
     3361 agttggtttt atgcgattca atctaccgcc gcagaggccg gacagcaggg atatcgtttg
     3421 gtcagcctgt tccgcggtgc gtctaccgat ttaaatttta cagtggccat tgccatcgtt
     3481 acggtcattt tgattcaggt gatcggtgta aaggcacacg gattcagcta tttccataaa
     3541 tttatcgata taaaagggat gattaccaat ccgaaaaagg gtttgatcaa cggttttgta
     3601 ggaattctcg aattaatcag cgaaatcgca aaaatcgcct cgttcggatt tcggcttttt
     3661 ggaaatatgt ttgcggggat ggtcctttta ttgtttctcg gatatatggt tccctggctt
     3721 gtaaatacgg ttatcttgtt gtatgaagct tttgtgggga ttctgcaggc attcattttc
     3781 ggaatgttgg caaccgtttt tatgagtatg gcagttcgat ctgaaagcta gattagttta
     3841 ttgaggggtg aaaaatggat ccacagattg gaaagtatat tggtgcaggc ttggtgatga
     3901 tttcaacgat aggagctgga attgcgatcg gaaatatcgg gaaaggtgca ttggatggaa
     3961 tttcacggaa tccggatgct tacggaaact taatgaccac gatgattttg gcaatcgctt
     4021 ttgccgaatc tttggcaatt tattcattgg ttgttgcctt cctgatttta ttcggataaa
     4081 aggatcaaaa tctcatggaa gctttaggaa ttaatgcagg caaccttttt gtcaatatca
     4141 tctgtttcgc gatcgcttat ctgatcatcg caaaattgat tgtcaagccg ataaaaaaga
     4201 tgacggacaa tcgcaaagaa gttattgata aaggaattgc ggatgcaaaa atcgcagcgg
     4261 atttacgtga aaatgcatta caagaatctg aaaaaattct caacgccgcc agagaagaaa
     4321 gtgccaggat gctgaaagaa gcgactgatc aaatcgccca gatgaaggaa gattatcgaa
     4381 aatcggttga tgcggaggta tccaaagaac tgagagattc acgtgaatat ttgaataaag
     4441 aacgtgagct gatgctcagt aatttacgcg gtcaaattat cgatctttct atcagcggtg
     4501 caaagaaaat cctcagcgaa gaactgagta tggatgaagc aaaacaacac aagttgttaa
     4561 atgacttgtt taccggtatt aaagatgggt ctataccggg gattgatcgg ctgcctaata
     4621 accttacaaa aatggaagtt actaccgcag tccccttgac ggatgaagaa cgagcgattg
     4681 ttgaagctca gttatccggt aagttggcgg agggggtnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnngcg gcgtttataa taaaactatg ccggtaaatt atctccaact
     4861 tcaatctcaa atacacattc tcagtgattc gattttaaat cagagagaaa aagggaaaga
     4921 ggcactggaa aaactgacgg atcaattcaa ggctgcagcc aacctgccgg atttagcgga
     4981 gagagtttcc gcaaaattac gagagaacag aggattaagt tgtgctctgc ctcgggaaga
     5041 aagaatcgat tctcatattt cagccagcga ccattcgggt gaaattgtaa atattttagc
     5101 cgccgatggt tcgcaaatta atcccagccg acatgaagaa gtcagtttcg gtttgattaa
     5161 tacggcaatt tttaaaatgc ggatgaacgg caatgaagca ccacaaatcg tcaccgaaac
     5221 aaagttactc gaacgggaag atgattcttt acaggagaaa ctgaataacg aagcaaatgt
     5281 cggtttattc cgtgatgtgg aagaacgccg gcttctttcc cgtgaagctt gtaaaattcc
     5341 gcaaggagaa ccggtcgttg cactgacaga cggccctctt cagctatatt atttccaaga
     5401 gccgaacact acggaaaatc ccttagtcaa tgaatactat aaagcattgg cagagatgag
     5461 tgagaaaaag attatcacgg cagggtatac ggatcgtcct cgctccacct ttgttatcga
     5521 tatgctgcgg ttgttgatga attcggatcc gcaggatttg agagttaaac agacgttggt
     5581 ttttgacagc aacctgtttt cacgcattct tttgcccggt gagcgctcgg cactttttaa
     5641 actgatttcg ccggctgttg aacaaatgcc ggcggtggtt cagatatcct ttttttattt
     5701 gaatgtgggc aatgattcac gcccgatgat tacccgtatt gaaattcccg aatgggtatc
     5761 aaaagatccg gctgcgattg atttgcttca ttattcgctt ttgcaacaaa acggattcat
     5821 ttcgtctaat ccatatccct atgctctgca tcgcgctcac gaatgtgcgg ttgtgcaatt
     5881 cagtgaaaag gaacatgtta aacaactgat catccgggaa ttgctttcac gcggtgtaac
     5941 cgatttggga aaatccgcaa agcagattgc aaaggattta ttcggaacaa cctaccgcga
     6001 ttttgaggaa taaaacagga aaatcggatc tttatacaga agaggggtct catgacagat
     6061 acaagcatgt ttggaagaat tctcagttcg aacattcggg agtgcgtcgt cggttatcgg
     6121 acaagcgaat ccgccattcc acctttcggt tccatggtcc gtattccggt aagtcatgct
     6181 cagcaaccgg aaatctatgg attggtgacg gatatccaga ttgaagaaga cggattcttg
     6241 cgtcaactgg ctaccaatga tcaggtttcg gaggaggtaa tgttggatgc ccgggcaaat
     6301 cgcaatgtgc cggttgtctt aaggattttg tttatcggct atctggaaaa cggaaatata
     6361 tcccatttat taccgccctg cccgccgttg acgttggata atatttataa atgcacgaat
     6421 gccgagatca cggcatttac ctctttcgga aattttggat acattcgaca cattctgaag
     6481 gtaacgaatg ttccgccggg tgaattgctt gcggcacata tcagtcaggc tgctaaggct
     6541 cagtttgctg ataatcattt cggttggacg gaaagagcca ttaatgaagt gatctattta
     6601 atgcgagata actatgccga tctgttagcg atattgggag cgatcggcga tgcggatatc
     6661 agtttggatg gagaagagga ggaacaggaa tgaccccggc agaaaatatc ggatttgtaa
     6721 ttgggggcag tttaaaagaa ggtctgtttg ttcgtctaac cgttcccgcc gaacgaattc
     6781 aggaaggcgc ttttgtcgtc atcgaaagca acggattgaa attttacgga ttggttgtta
     6841 atctcagtct caaatcttat gatcccagat attcgcaaga tttaatcgcc ggcagaatgt
     6901 ccgataaaat tacgacttta ttgcataacc agaccctttt taccgagatg gaagttttac
     6961 cggttctgat gcaaaatacg ggtccggaac tcgaagatcc gaattatgat cccaatgccg
     7021 tgatggataa atcacccatg ccggttaaaa ctctccccat tcatcaggca cccgttcggc
     7081 ttgcaaccga attcgacgtc agcgaagtgt ttaaacccaa agaaaaaaat ggattcgtca
     7141 tcggtaccac gcgcgagcaa ggacatccag ttcatatcga tatggataaa tttattcagc
     7201 gctcttcggg aatttttggg gcgaccggaa cggggaagag ttatttgacc cgcatgatct
     7261 tagccgggtt aattcaaagc aaacaagctg ccgtgctggt ttttgatatg cacaatgaat
     7321 atgcatacgg cgatatttca cccgatacgg atataaaagc gccgggtttg tgcgaaaaga
     7381 tgaaaaaaag agtcaagtct tgtgctttgg gagcttcagc aaaaattgca ggcaatcaac
     7441 ccgattttga tttggtactg gaatattcgg atattctacc gtccgatatt gagatgctga
     7501 cccgggaatt gaatctgcgc gaaacgactc cggctacgct gtcggcgttg gttcaatcct
     7561 tcggtataaa taaatggttc gagtcattca aaaatctaac aatgggtgag ggagagggtt
     7621 cggtcgtgga ctgggcgcgg aacaacaatg tgaatgaagc tgccgcactg agtctgtata
     7681 gcaaacttga acgggttttt cggttgaact atcttgttcc gaagttgacc gggccttctt
     7741 cgattgtaaa aataatcgat tatttaaaaa atgggtattc ggttattttg tcttttggag
     7801 aatatgagac ggacttggat tatttgttgg tgacaaacat tctgacacga aaaattcgtg
     7861 aagaatggga acgaatgacg aatgaatatc gaaatcaagg cggtgtcaaa cctactccgc
     7921 tggtgattgc attggaagaa gcacataaat tattaaaccg ggaaatggcc ggacaaacaa
     7981 cattttcaac aattgccaga gaaatgagaa aatattttgt gactttactc attatcgacc
     8041 agcgtccatc tcaaatttat gacgaagtga tgtcccaact gggaactcgt atctcaggct
     8101 ggctgggaga tgatgaggat atcgcagcag tactatccgg actttccggg cgggattcat
     8161 tacggggaat gttagcccga ttgcaaccga aggaggaagt tcttcttctc ggttacggcg
     8221 ttcctatgcc cataccgatt cgttcccgtc ggtatgatgc tcaattctgg caagaaatgc
     8281 tgaatgagga tgagctttcc gcgacggata aacaaaaatt tcgggaaatg atttttgacg
     8341 atttttagga atctttcaaa aaccaatgtg agaatttcat gactgaggcc ggtccaaacc
     8401 gatcgataga aagacaaaaa gagccgaaaa ttttggtgat ctcattattt ttggcgattc
     8461 aatttgctct gtgtatttat cttcgatgga ttccttatcc gatccactca aaaagagcct
     8521 tagcggcgat gatccttttg atcggactgt ttacgatttg tacagacttt ataatatccc
     8581 gatggatcct tatccgtttt cggagaacaa cgaaaacggt tttttgcttt acccttttag
     8641 ccagtttgat cttcggggga ttactgcttg ccgttcaaac cgttccggtt cccgatcgat
     8701 atttcttcct tcccgacggc tcggtaaaaa ttacagccat cgctgaaaaa aatccgcttt
     8761 caaacggaaa gaaagtcgaa atattgtatt ttgacaccgg gacaacggat aatataaacg
     8821 ctttcgaaca ggcgggaaat tggtcggtac aaaacaaaac cgttgtaacg gaagaagagg
     8881 gatcgcttta ttggaacgga aaagtttttc atcggattca gcttttattt gccagcgggc
     8941 cagattgcgg aatcgttcgg atcgattggg cggggaaaag ccagcggatc gatctctatg
     9001 cggaacaagc gggagaaatt ctatttactc aggatttccc gttttcttgg atcaatcgcg
     9061 ctgttattat ttcggcatat tggattggta tttcttttct ctttctgtta tttctgactt
     9121 taacagttcc ggtaaagtcg caaaagaaat tcgccgaagc ggaggcaatt ccggcttggt
     9181 gggtatttgc tttaccgatg atttttgtat ggacaatttg cctgttaact ttttggcccg
     9241 ggattttaac tccggattcg gtcgtccaat ggggacaggc tgttgcaaag gtgccgcttg
     9301 ataatcggaa tcccgccata tatactcttc tcatcaggtt gttgaccacc gtttccaaca
     9361 ccccggcaat tgtggctttc gctcaaattc tcgccttaag tttggttact gccggcggaa
     9421 ttcgtttcct tttgaatcaa catatttccg ttaaaacagc ttggatattg agcataatca
     9481 tggctttttc gccggttaat cttttcttcc cgatcacgat ttggaaagat gtgccttacg
     9541 gaatcagtat gcttgccgtt tttctgatct tattgcaaat cgtaatcagc gacggagttt
     9601 ggctgaaaaa acgttcgaat cggatttggt tgatattgtc cggaatttgt ctttctttat
     9661 tccgtcataa tggatttccg gttcttgtgg ccacattagt catttttctg ttgttttatc
     9721 gtcaaattcg cggtttatta tttgaggcct tggtcgtcat tattgcagcc tatggccttg
     9781 ttctcggtcc ggtttactcg gttttccatg tgacaaaagg caacgataac ggaatcaacc
     9841 cgattttggt tcattatatc agcgctcata ttgaggctga aacacccctt agtgaatcgg
     9901 agcgaacctg gctgaatcag gtttaccctt tggatcaatg gaaatatgat ccctgtgtaa
     9961 tcaataatct atggtttctt cccggattta atgaaaaagt gctgcaagaa aatccgaaag
    10021 aagtgatgaa cattttcctt tcgctttcaa aaaaggcacc ggcagtcagt ttgcgccatg
    10081 catttcagtc cagcaatatg atctggaaga tccagcacgg cggatgttat ttatatcgaa
    10141 tcggtttact gggacaatcg gacggaactt taaaatgggt ggaagcaaat caaatcggtg
    10201 tagttcagga atccaagctc ccgaaattaa ccgatcttct tttcaacttt tttctgaaaa
    10261 cggcaggtcc gacattcccg gatgctattt tttggcgtcc cgcaatttat acatactgga
    10321 cgttattcgt cagtaccatt ttggctttgc gtttttctaa acccaaatac ctcttgattg
    10381 gattattgac tttggtccaa tcgggtatga tgctgtttat cacgattgct caggatatgc
    10441 gctatcaata cggggtcatg ctgatcggta tcttttgta
//
LOCUS       DAOSMM010000068        10109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_6657_length_10109_cov_10.028645, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000068 DAOSMM010000000
VERSION     DAOSMM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6657_length_10109_cov_10.028645"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1088
                     /locus_tag="PK898_08695"
     CDS             66..1088
                     /locus_tag="PK898_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU20148.1"
                     /translation="MTCSTAAILIASGNIIHPKNTVGRFFAPDFNKKPSGINTSTNNG
                     TESEIGAKMAENVYMTTVISPTGLMNIYKALGRKAGGHVAVKISSGEPGGYYFLSPAL
                     IAPLVSEVKGTIVECNTAYGGGRSNTASHLKVMKEHGFTDIAAVDIMDADGTMNLPIS
                     GGSHLKEDVVGSHFKLYDFIINLAHFKGHAMGGFGGVIKNMSIGIASSKGKSLIHSAG
                     KETTGFGINTPQDDFLESMAEAAKGVADYLGDRILYINVMNNLSVDCDCDSHPSKPTM
                     KDIGILASLDPVALDQACVDLVYAAPDGKDLIERIESRHGIHTLEHAEEIGLGSRQYQ
                     LVSIDS"
     gene            1104..1526
                     /locus_tag="PK898_08700"
     CDS             1104..1526
                     /locus_tag="PK898_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU20149.1"
                     /translation="MKNFDLPNTGFFAKGDPLPEPLRKNFSGACYLNPIIPANSPLGC
                     SVSNVTFEPACRNNWHKHPGGQILLVTGGRGWYQEEGKEARELHAGDAVEIHRNVKHW
                     HGAAKDSWFSHISIETDPKAGPAEWLEPVSDQEYNQLD"
     gene            1590..1997
                     /locus_tag="PK898_08705"
     CDS             1590..1997
                     /locus_tag="PK898_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU20150.1"
                     /translation="MKIAEVSKMYGISSDTLRYYEKVGLIPAVNRDASGIRDYTETDL
                     KRIEFVMCMRNAGLPVQILAEYMHLIMQGDETIGSRKEILVKQRESLLTKMNELQKSL
                     DLLDYKINVYENALLKKEKEMVDDMEIAVERRI"
     gene            complement(2103..3689)
                     /locus_tag="PK898_08710"
     CDS             complement(2103..3689)
                     /locus_tag="PK898_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20151.1"
                     /translation="MRNLKRHSENTFFFIAFLILYLAFAYFFTVILDTHYNDTVSRTA
                     LGYMVIYGRYPKLANVGFVWTPLLSLLQLPLFPLLRLFRHPDLAGPIITAISGAATIP
                     VLNSIGKDLNVNKRIRWITILLFGLNPTVVLYTAVGMSETFFFLPFALCILYLERWLT
                     GKRHYTALAVSGSALAVAFWSRYEAIPILFAVCIVILLYVLEHPLPSVSLYRLSEAGI
                     ITFALPVIYSVFLWIFFNWTIMGDPLYWVTGEYSNTAYTTSFQSQMNPLFHNLLTSIT
                     YAFERIILISPLLIILTIVAIILCYQKRSWMPISIFIPSLVMIAFHIYQTFGGVSYGW
                     YRFYSYGLIGSVWGFFWLWHFLADQKKYRPIFEIISIGIMAITCFTTTVAMLNPNIGK
                     EENSLISALVPGGSVSTDESRSYASAKAVNEFLRENNKNNDLVLIDSFQGFAVILYAD
                     NPNIFVKTQDEDFQKALRDPIEYGIQWVLVPKPDESLRYSEWMYRIYPGIWEDPPSWL
                     VMVKDFGAWRIYKVMPREIG"
     gene            complement(3712..7692)
                     /locus_tag="PK898_08715"
     CDS             complement(3712..7692)
                     /locus_tag="PK898_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU20152.1"
                     /translation="MVSEARNRIYPIRKLRIPKYLSGILPEHIADQYCLVPVAISDDH
                     LIIAAAYRLSSNARNEIEKITRYPLEIISASFQDIRSVQSWLYQREYDQPKPVLPEVA
                     LNKMGLLNNEQIQSLKKDQNTSIKDLPALAFENHLINEAQWGEVVGLCLNSPHFSSRN
                     AAPIPGISSLSFLISEKSNLLPVWIVKNEFYLATSDPGELGSSLGGKVNFDSYRVSFC
                     APSLFNTLKQRIYSYVPDQIIVDDDKIAQTLVKRGWLSENDLSISVEICRRLKRPLQS
                     VIRERYRITSKQWLEASGEVLKLPFIHKDSIKKNQLAEIFDLLEMLPVDLFYQKKAFP
                     FSVTENHIKLAIIMPDSQLIRLLESISGLTVDVYLTDEETLNILFDRYQLLSKEHYPR
                     EFEKNAISFADFLLGTGILQKTQFLELEKKYDWQKKSLSTALIETKIFDELEIAELLS
                     FFYYIPSLSLDHFQFDDQLINQIPKKFVQEKNILPLFENNDDLWVAIGDPEKDTEYFW
                     ELEQYTHKRIWPVISPASLIKKTVKLFYQNGRNNIDNKEVYKLLDQLVNRGVINWNDA
                     NQVIKDLLERQTPIDQALLRLGKLDQKEIAKSLSAVLNVEYKSLALQEIKATYIDPLG
                     KEIVRHEWVDPVDLNTAQLMDLKTAQRFCALPISRNQDHVLVAFADPLFESAQNELSI
                     LLNRKVVPCLSPRDELEAAIQRTLGHVNLGTALLTAGLITLSQLNDGLTLAKQTNVRL
                     GRALVYRRYITEDQLYQFLANQAHLPVFELSKVELDQEIARLIDADIERKLGILPLIQ
                     NNGILTMAITDPLNDEAIRYAENTTGMKINPVLVTERDFDLAMERLYKNEYLNQSISR
                     LLERTPEDSAYHLFSTGQIIAIVFFVIISILCLKINYLQYLIVINAFATLFYTLFSLY
                     KGYIIGKALSNDLEVSVSNEEVDALDEKELPVYTILVPVHKEANVLGKILKRLSQMDY
                     PITKLDIKILLESDDHETIDAFYDANPPAFIQEVIIPVSAPRTKPKACNYGLIHAKGE
                     YVVIYDAEDLPDRDQLKKVVVAYQKVPDDVVCIQAKLNYYNRNQNLLTKWFTSEYSMW
                     FDLLLPGLDATRAPIPLGGTSNHFRMSSLLEIGAWDPYNVTEDADLGMRLYKRGYRTR
                     IVDSITLEEANSQIGNWVRQRSRWVKGYIQTWLVHMRHPFRLWREVGSGAFFSFQMLI
                     GGTFFALLMNPIYWTMTVLWYLTHAGVVKAINPGIIFFMGSICLYFGNFIFTYANIAG
                     AIRQKYYDLVRSALFSPIYWALMSIGAWRGFLQLFFKPHFWEKTRHGLSGSDQSDSQN
                     QKAPAR"
     gene            complement(7929..>8335)
                     /locus_tag="PK898_08720"
     CDS             complement(7929..>8335)
                     /locus_tag="PK898_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20153.1"
                     /translation="DSRSYERLAEKPVKSGFDSFSYRKQDEKVISEPETKISDLMRVS
                     RAERKDALDKGEYFLEFYNFSSLSHLSELEKIIRGSDHFDNYKILKLKGNTLTAKVKL
                     AGGDIVDILQSSLGKAVRGIERDDNIIRIVLA"
     assembly_gap    8336..8435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8436..9412)
                     /locus_tag="PK898_08725"
     CDS             complement(<8436..9412)
                     /locus_tag="PK898_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20154.1"
                     /translation="MKIIINKARRLGFYPIILVLWSVIAAVGLITLSPQMGFMAIAIF
                     VFIVVISLTDLFPFSSIVAVLIGVGAYFTIFTSMYSINRNSIIQIGIVTVIYAITAFL
                     SNRYIRQVSLLEQEADRQQFLMDDLILYDQLTGVLRWKYARRQLSGEVSRSRRYKKDL
                     SLVILQPVISQDQKMNEDELNNLYTRIMEIISRTIRKDVDIPFMGEKNGIILPETNSE
                     GARIVCARLADNVFRKIRVDLAFGISNFPDDAVTDDDMVTNAEMALKYAISSRQSIVP
                     FSKVYERPVENQKNGYDSKPFEQSTEKSTIETDSRSYERLAEKPVKSGF"
     gene            complement(9393..>10109)
                     /locus_tag="PK898_08730"
     CDS             complement(9393..>10109)
                     /locus_tag="PK898_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20155.1"
                     /translation="PYIFMGLSDLSQLAFVMPDKTSITDLQSLSILALAFGKSSAGDP
                     LMPHVIPASQISQNEEKYPYQVLIGLPLNHSAFLSQNDQIPLKFDPSTGAAEPLPVLD
                     TIESGKISTGYVEAYVSQSNIPRLILTGNDEEGLLLAANLLNDNTAVINLNGDVAITT
                     GKDRVISFWGKMFPISTSNDSVIMQDQLSLSMILFQKNAAFYTAILFLAFSILICIIR
                     LFVVLRSKKINKEEHENNNK"
BASE COUNT         3156 a         2016 c         1938 g         2899 t
ORIGIN      
        1 tcgacccgga aaggatagaa actcggatga aaaaaacaat ttctcgcagg gaagcgctga
       61 aacggatgac ttgctcaacg gctgcgattc ttatcgcatc cggaaatatc attcatccga
      121 aaaacaccgt cgggcgtttc tttgcgccgg atttcaataa aaagccttca ggaataaata
      181 catcaacaaa taatggtaca gaatccgaaa taggagcaaa aatggctgaa aatgtataca
      241 tgacaacagt gatcagtccc accgggctga tgaatattta taaagcactc ggtcgaaaag
      301 ccggcggtca cgttgcggtg aaaatctcca gcggagagcc gggcgggtat tacttccttt
      361 cacctgcatt aatcgcccct cttgtctcag aagttaaggg aacgatagtc gaatgcaata
      421 ccgcctacgg aggtgggcgt tcgaataccg cttctcactt aaaagttatg aaagaacatg
      481 gctttacaga tatagcagct gtcgatatca tggatgccga tggaacaatg aacttgccga
      541 tctcgggagg aagccacctg aaagaagacg tcgtcggatc acattttaaa ctttacgatt
      601 ttataatcaa tttggcccat tttaaaggtc atgccatggg tggattcggc ggtgtgatca
      661 agaatatgtc catcggaatc gcttcgtcaa aagggaaaag cttaattcat tcggcgggaa
      721 aagaaacgac cggtttcggc atcaacacac ctcaggatga ttttctggaa tcgatggcgg
      781 aagccgctaa aggagtggcc gattatttag gtgatcgtat tctttatatc aatgtaatga
      841 ataacttatc cgtagattgc gattgcgaca gccatccttc gaagccaacc atgaaagata
      901 tcggtattct cgcttcgctt gatcctgtcg cgctcgatca agcttgcgtt gatctcgtat
      961 atgcggcacc ggacgggaaa gatttgattg aacgtatcga atcacgtcac ggaattcata
     1021 ctttggaaca cgccgaagag atcggcttgg gtagtcgtca atatcaatta gtcagtattg
     1081 attcataaaa aaggggtaca tttatgaaaa actttgattt accgaacacc ggattttttg
     1141 caaaaggcga tccgcttcct gaacctttaa ggaagaactt ttccggggca tgctatctca
     1201 atccgatcat tcccgcaaat tcaccgttgg gttgttccgt cagtaatgtg acatttgaac
     1261 ctgcttgtcg taataattgg cataaacatc cgggtggaca aatccttttg gttaccggtg
     1321 gaagaggttg gtatcaggaa gaaggaaaag aggctcgtga attacatgcc ggagatgcgg
     1381 tggaaattca tcgtaatgtg aaacattggc acggagcagc caaggacagt tggttttctc
     1441 atatctcaat cgaaacagac cccaaagcgg gacctgccga atggttagaa ccggtctcgg
     1501 atcaggaata taatcaactg gattagtttc gattttttat cgatagggct cgccggctcg
     1561 attcttacaa cctgtattga agggaaatca tgaaaattgc tgaagtcagt aaaatgtatg
     1621 gaatttcatc ggatacgttg cgctattatg aaaaggtcgg actgattccg gcagtcaaca
     1681 gagatgcaag cggcattcgc gattatacgg aaacggatct gaaacggatt gaatttgtta
     1741 tgtgcatgcg gaatgcgggt ctgccggtac aaatattggc ggaatacatg catctcatca
     1801 tgcaaggaga tgagacgatc ggctccagga aagagatcct cgtaaagcaa cgggaatcac
     1861 ttttgaccaa gatgaatgaa ttacaaaaat ctttggattt gttggattat aagatcaatg
     1921 tctacgaaaa tgctcttctc aaaaaagaga aagaaatggt tgacgatatg gagatagcgg
     1981 tagagagaag aatttagaag ttttcagtga cttataatta gaagaatctg cctacagatt
     2041 cttctttttt tgcgaatccg tttagacagc tttgtattat aacgatgagc gatcgcaaac
     2101 tcttatccga tttctcttgg cataaccttg tatattcgcc atgctccgaa atctttgacc
     2161 attaccagcc aactcggcgg atcctcccaa ataccgggat agatccgata catccattcg
     2221 ctgtaacgca aggattcgtc gggtttcggc accaataccc actgaatccc atattcaatc
     2281 ggatctctta aggctttttg aaaatcttcg tcttgcgttt ttacaaaaat attgggatta
     2341 tcagcatata aaatgacggc aaagccttga aaagaatcga tcaaaaccaa gtcattattt
     2401 ttattgtttt cccttaagaa ttcgttcaca gccttggctg aggcatagct acggctttca
     2461 tcggtcgata ccgatccgcc cggtaccaaa gcggatatta aactattttc ttctttgccg
     2521 atattgggat tcaacatcgc aaccgtagtg gtaaaacagg taattgccat tattccgatt
     2581 gaaataattt caaaaattgg gcgatatttc ttttggtcgg caaggaagtg ccaaagccaa
     2641 aaaaatcccc aaacgctgcc tattagacca tatgaataaa aacgatacca tccgtaagaa
     2701 acaccgccaa acgtttgata aatatggaaa gcgatcatta ctaaggatgg aatgaatatg
     2761 cttatcggca tccaggatct tttttggtaa cacagaataa ttgccacaat tgtcaaaata
     2821 atcagcagcg gtgaaatcag aattatcctt tcaaacgcat atgtgatcga agtaagtaaa
     2881 ttatggaaca atgggttcat ctgagattga aaagaagttg tataagcggt attcgaatat
     2941 tcgcccgtga cccaatataa aggatctccc ataattgtcc aattaaaaaa gatccataaa
     3001 aaaacactgt aaatcaccgg taatgcaaaa gttatgatcc cagcttcgga tagacggtag
     3061 agagagacgg aagggagagg gtgctcaaga acgtataaca aaatcacaat acatacggca
     3121 aataaaatag ggatcgcttc gtaacggctc caaaatgcaa ctgccaaagc actaccggaa
     3181 accgctaacg cggtataatg ccttttgccc gttaaccatc tttcaagata taagatacat
     3241 aaagcgaaag gcaaaaaaaa gaatgtttcg ctcataccca ccgctgtata gagtactact
     3301 gtcggattca gtccaaaaag aaggatggtt atccatctga tccgtttatt tacattcaga
     3361 tctttaccga tcgaattaag tacgggaata gttgcggctc ccgagatagc tgtgatgatc
     3421 ggtcccgcca aatctggatg tcggaacaac cgtaataatg ggaagagtgg caattgcaac
     3481 agactaagta aaggtgtcca tacaaaaccg acatttgcca gtttgggata cctcccataa
     3541 attaccatat aaccaagagc cgtacgagaa acagtatcgt tataatgtgt gtctaatatc
     3601 accgtaaaga aataagcaaa tgccaaatag agaattaaaa aagcgataaa aaaaaatgta
     3661 ttttccgaat gtcgttttaa atttctcatg atatttcttt tcaaatgaaa gttatcgagc
     3721 aggagccttt tgattttgtg aatcggattg atcgcttccg ctcaacccat gtcgggtttt
     3781 ttcccaaaaa tgaggtttaa agaataattg cagaaaaccc ctccaagcac cgatactcat
     3841 taatgcccaa taaatcgggc tgaataatgc actcctgaca agatcataat atttttgacg
     3901 aattgcaccg gcgatatttg cataagtaaa tataaagttt ccaaaataca gacatattga
     3961 acccataaag aaaataattc cgggattaat tgcttttaca acacccgcat gtgtcaaata
     4021 ccataatacc gtcattgtcc aatagatcgg gttcatcagc aaagcaaaaa aagtccctcc
     4081 gatcagcatc tgaaaactga aaaaagctcc ggatccgact tctctccata gtcgaaaagg
     4141 atgtcgcata tgaaccaacc aggtctgaat ataacctttt acccaacgtg atctttgccg
     4201 gacccagttg ccgatctgac tgtttgcctc ttccagtgtg atactgtcta caatgcgagt
     4261 tcgatatccc cgtttgtata accgcattcc caaatcggca tcttccgtaa cattatacgg
     4321 atcccatgcg ccgatttcaa gcaacgacga catacgaaaa tgattcgaag tcccgcctaa
     4381 aggaatcgga gcccgagtcg catccaatcc cggaagcaat aaatcaaacc acatagaata
     4441 ttcggaggta aaccattttg tcaacagatt ttggttacga ttgtagtaat tcaatttggc
     4501 ttggatacac actacatcat ccggaacttt ttgatatgcg actactactt ttttcaattg
     4561 atcacgatca ggcaaatcct cggcatcata aataaccaca tattcgcctt ttgcgtgaat
     4621 taatccataa ttgcaagctt tgggctttgt tctgggggcg gaaaccggga tgattacttc
     4681 ctgaataaag gccggcggat tcgcatcata aaatgcatca atcgtctcat gatcatcaga
     4741 ttccaataat atctttatat ctaattttgt aatcggataa tccatttgag aaagacgttt
     4801 aagaattttt cccaaaacat ttgcttcctt atgtaccggg actaaaatcg tataaacagg
     4861 cagctctttc tcatcaagtg catcaacttc ctcattactg acggaaactt caaggtcatt
     4921 cgacaacgcc ttgcctatta tatatccctt ataaagggaa aaaagtgtat agaaaagggt
     4981 agcaaaagca ttaattacaa tgagatattg cagataattt attttcaaac ataagattga
     5041 aattatgaca aaaaaaacaa tggcaataat ttgaccggta ctgaagagat gataggctga
     5101 atcttccggt gtccgttcca ataatcgaga aatactttga ttcaaatact cgttcttata
     5161 taagcgttcc atcgccaaat caaaatcccg ctccgtaacc aaaacaggat ttattttcat
     5221 tccagttgta ttctccgcat agcggatagc ttcgtcattc agcggatcgg taattgccat
     5281 tgtcaaaatg ccgttattct gtatcaaagg taagattcca agcttccgct cgatatctgc
     5341 gtcaattaag cgggcaatct cttggtccag ttcgactttg gataattcaa aaaccggtaa
     5401 atgagcttgg tttgctaaaa attgataaag ctgatcttcg gttatataac gccggtatac
     5461 aagcgctctt cccaaacgga cattggtctg tttcgctaaa gtcaggccgt cattcagttg
     5521 acttaatgta attaatccgg cagtcagcaa agctgtcccc agatttacat gtcctaatgt
     5581 ccgttgaata gcggcttcca gttcatcacg aggagataaa caaggcacaa cttttcgatt
     5641 caataatatt gaaagttcat tttgggctga ctcaaacagt ggatcggcaa aagcaaccaa
     5701 aacatgatct tgattccgag aaatcggtaa agcgcaaaat cgttgagccg tcttcagatc
     5761 cattaattgt gccgtattta aatcaacggg atcaacccat tcatgacgta ctatttcttt
     5821 acctaaagga tctatataag ttgcctttat ctcttgcaac gctaaacttt tatattccac
     5881 atttaaaaca gcggaaaggg atttggcgat ttctttttga tcgagtttcc cgagtctcaa
     5941 taaagcctga tcaatgggag tctgtcgctc caatagatct tttatgacct gattggcatc
     6001 attccaatta attacgcctc gatttaccaa ttgatccaga agtttataaa cttctttatt
     6061 gtcgatatta ttcctaccgt tttgataaaa caacttgact gttttcttta tcaacgacgc
     6121 cggtgaaatt acaggccaga ttcgcttatg agtatattgt tccaattccc aaaaatattc
     6181 ggtatctttt tccggatcac cgatcgccac ccataaatca tcattatttt cgaataaagg
     6241 gagaatattt ttttcctgca cgaatttttt cggtatttga ttgatcaatt gatcatcaaa
     6301 ttgaaaatga tccaaagata aggatggaat ataatagaaa aagcttaata attcggctat
     6361 ctcgagttcg tcaaaaattt tcgtttcaat taaagccgtg ctcaatgatt tcttttgcca
     6421 atcatatttc ttttccaatt caagaaattg agtcttttga agaataccgg tgcccaacaa
     6481 aaaatcggca aaagaaattg catttttttc aaattcacgg ggataatgtt ctttggagag
     6541 caattgatac ctatcaaata atatatttag agtttcctca tccgtcaggt acacatcaac
     6601 cgtaagcccc gatatacttt ccagaagccg gattaattga gaatcgggca tgataatcgc
     6661 caatttgata tggttttcgg ttacggaaaa aggaaatgcc tttttttgat agaatagatc
     6721 aaccgggagc atttctaata aatcaaaaat ttccgccaat tgatttttct ttattgaatc
     6781 tttatgaata aacggcagtt ttaaaacttc ccctgaagct tcaagccatt gttttgaagt
     6841 aattcgatat cgttctcgga tgacagattg cagcggccgc tttaatcgcc tgcaaatttc
     6901 aacggatatt gagagatcat tttccgaaag ccatcctctt ttgaccaaag tttgggcgat
     6961 tttgtcatcg tcgactatga tttgatccgg aacgtatgag taaatcctct gctttaatgt
     7021 gttgaataaa gaaggtgcac agaaggatac cctataagaa tcaaaattaa ccttaccgcc
     7081 caaggaagat ccaagctcac caggatcgga tgtcgccaaa taaaattcat ttttcactat
     7141 ccataccggc agaaggtttg atttttcaga gataaggaac gataaactac tgatccccgg
     7201 gatcggcgcg gcattacgcg aagaaaaatg gggagaattt aaacaaagac cgacaacttc
     7261 accccactgg gcttcattta ttaaatgatt ttcgaatgca agtgccggta aatctttaat
     7321 agatgtattt tgatctttct taagtgattg aatttgctca ttattaagca gacccatttt
     7381 attgagtgca acttccggta gtaccggctt cggctgatca tattctcgtt gataaagcca
     7441 tgattgtacc gagcgaatat cttggaaact tgcagatatt atttcaagcg gatatcgggt
     7501 aattttttca atttcattcc gggcatttga tgacagacga tatgcagccg caatgattaa
     7561 gtgatcatca gaaattgcaa ccggtaccaa acaatattga tcggcaatat gttcaggaag
     7621 aattccggaa agatatttgg gaattcttaa ttttctgata gggtaaattc ggtttctcgc
     7681 ttccgaaacc ataattttgc ttcatgcctt tcaaattatt attcagaaaa aactctgatg
     7741 cttccttctt ttctgttggt agaccgcatt cataatcgta gaaaaatatt caggaaatga
     7801 tcagaaccaa taaaaaatcc ggtttgaaat tcaggacaaa caaaacagat ttgtggattt
     7861 ggaaaccatc cggctgtaaa tcaaataaaa ttttccttat tttcttgaat aaattcgggg
     7921 aaggagagtc aagctaatac gattcgaata atgttatcat ccctttcgat tcctcggaca
     7981 gcttttccga gcgaagattg aagaatgtct acgatatccc ctcccgccaa tttaactttt
     8041 gccgtcaggg tatttccctt taatttaagg atcttataat tgtcaaaatg atctgatccg
     8101 cggattattt tctctaattc cgacaaatgg cttagggaag agaaattgta aaattccaaa
     8161 aaatattcgc ccttatccaa cgcatctttt ctttccgcac gggatactcg cattaagtcc
     8221 gagatctttg tttcgggctc ggaaatgact ttttcgtctt gctttctata tgaaaatgaa
     8281 tcaaagccgg attttacagg cttttctgcc aacctttcat atgatcttga atcagnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcaaa gccggatttt acaggctttt
     8461 ctgccaacct ttcatatgat cttgaatcag tttcgatcgt tgatttttcc gttgattgct
     8521 caaatggttt tgaatcgtac ccattttttt gattttcgac cggtctttca tacacttttg
     8581 agaaggggac gatagattgc cgagaagaaa tagcatattt caaagccatt tcagcatttg
     8641 tgaccatatc atcatcggta acagcgtcat cgggaaaatt cgatattcca aatgctaaat
     8701 caacacgaat ttttcgaaaa acattatctg ccaaacgggc gcatacaatc cgcgcccctt
     8761 cggaattcgt ttcgggaaga ataattccgt ttttttcacc cataaagggg atatcaacat
     8821 ctttccgaat tgttcgtgaa attatttcca tgattcttgt gtataagtta ttcaattcat
     8881 cttcattcat tttttgatct tgcgaaataa caggttgtaa aatgaccaaa gataaatctt
     8941 ttttatacct tctgctgcga gaaacttctc cggaaagttg tcgacgggca tatttccatc
     9001 ttaatacacc ggttaattga tcgtaaagaa ttaaatcatc catcaagaat tgctgtcgat
     9061 ccgcttcttg ttccaacaga cttacttgtc gaatatatcg atttgaaaga aacgctgtaa
     9121 ttgcataaat aacggtgaca attccgatct gaatgatcga atttcgattt atcgaataca
     9181 tactggtaaa aatagtaaaa tatgctccga ctccgatcaa aacggctacg atcgaagaaa
     9241 aaggaaataa atcagtcaga gaaatcacga caataaagac gaaaattgca attgccataa
     9301 accccatctg aggggataaa gtgatcaatc ctaccgccgc aataacactc cataacacga
     9361 gaataatggg ataaaatccc agccgtcgag ccttatttat tattattttc atgttcttcc
     9421 ttattgattt tctttgaccg aagaacaaca aacaatctga tgatacatat caatattgaa
     9481 aatgccaaaa agagaatagc agtataaaaa gctgcatttt tctggaataa tatcatcgat
     9541 aaagaaagtt gatcttgcat gataacggag tcgtttgacg tcgaaatcgg gaacatttta
     9601 ccccaaaagc tgattacccg atcttttccg gttgtgatcg caacatctcc gtttaagttt
     9661 atgacagcgg tgttatcatt caataaattt gcagccaaca aaagtccttc ttcatcattc
     9721 ccagtgagaa ttaaccgcgg aatattagat tgactaacat aagcttctac ataaccggta
     9781 ctgattttcc ccgattcaat tgtatccaaa accggcaaag gttctgctgc tccggttgag
     9841 ggatcaaact ttaatgggat ttgatcattt tgggacaaaa aagccgaatg attcaaaggt
     9901 aagccgataa gtacctggta agggtatttt tcttcatttt gagaaatttg tgatgccgga
     9961 atcacatgag gcatcagcgg atcacccgcc gatgatttac caaaagcaag cgcgagaata
    10021 gataatgatt gtaaatctgt aattgaagtt ttatctggca ttacaaatgc taattgagat
    10081 aaatcactca atcccataaa aatataggg
//
LOCUS       DAOSMM010000069        10027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_6715_length_10027_cov_11.980646, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000069 DAOSMM010000000
VERSION     DAOSMM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6715_length_10027_cov_11.980646"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1139
                     /locus_tag="PK898_08735"
     CDS             360..1139
                     /locus_tag="PK898_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU20156.1"
                     /translation="MADDYKMMHLGIKKGDVGRYVFLPGSVERATLISEYFDHPRKIA
                     HNREFLTYSGTLDGVLVSVTSTGIGGPSTTIAIEELFQCGADTMIRVGSCASTSPIAR
                     IGDVMIPNGSVRMEGTGVHYLPMEFPAVPDFFLLKELEQAAIRLNFRYNVGINICKDS
                     FYTEVSPETKPVYYELKAKWEAYEKGGATHTCMECAPLFLAGASLGIRTAAVLICATN
                     YKSYSNDDADYPRGFEHNAIKTGIEAMRSVIHKDRQKEQAN"
     gene            1153..1920
                     /locus_tag="PK898_08740"
     CDS             1153..1920
                     /locus_tag="PK898_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU20157.1"
                     /translation="MDDYKMMHLGIKKGEVGAYVFLPGSPERSEKISKYFEHPVKVAY
                     NREFLTYTGTLDGIPVSVTSTGIGGPSTAIAVEELFSCGAHTMIRVGTCASTSPKVGI
                     GDIVIPNGAVRMEGVGLHYLPIEFPAVPDYFLVKELESAAIKLGYKYNIGITISKASF
                     YTQTNPETKPVANELIEKWSAYEAGGATSTEMESAPLFLVGSSLKIRTAAVLVSATNF
                     HEYSNDKRNYPGDLEHRAIETGIEAMRAVIRKDMDRE"
     gene            1913..2740
                     /locus_tag="PK898_08745"
     CDS             1913..2740
                     /locus_tag="PK898_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU20158.1"
                     /translation="MSNLEEKTELSVEKQLDGFGKIELSNIPQIDRTVLDGVPIYVRI
                     CDIFYHLVEKGVANAGDQLPGENLLAEHWNVSRATVRQAFRHLEEDGLVYKRQGKGTV
                     IAFNASSLHHSLPWCYNICLENCIEQIDSVEMKSNVEMCGEYLADRLEATPGKELQIM
                     DFYYRSQYEMMSTSVLMVMVEKLKYFGINPENRDDLYRFGQEGIYSFAATAQSFMTAF
                     GANADKNMKMTVPKNDVVLFLEEIMRDEKDQPIGYARYYLRSSCYRIPINRRAVMSY"
     gene            2798..3889
                     /locus_tag="PK898_08750"
     CDS             2798..3889
                     /locus_tag="PK898_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU20159.1"
                     /translation="MSKKLFSVMLAIAVVFSIFGIVAAEDAPIKVVLLINGSLGDKGF
                     YDSAASGVRRMEKELGAQTKIIEMGRDETAYEGHYLDVSEQDWDLIISGTWSVKELAQ
                     NIAVQFPEKHYIFFDGNVDRTVVTTGNMMGISYLDNEGSFMAGALAALMLDSGAEKID
                     PDKRILGFVGSMDTANINNFLVGYIEGIKYIDPSIKLLTSYVGSFEDVPKALEMTTQL
                     YNQGAQIVFAPASQSILGAVTASSNVDKYFIGCDMDTWAQLQDTDANLARNIISSALK
                     NVGDSLVTAVKGYMDGTMDTSHDYSLGLSTGAVGLAKNDNYKALVPEEIQKKLDEIEK
                     KIIDGEIVVGSAFGMKSEDVAALRDAMKP"
     gene            4057..5616
                     /locus_tag="PK898_08755"
     CDS             4057..5616
                     /locus_tag="PK898_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20160.1"
                     /translation="MEKTAEALRLQNITKVYPNGVIANNGVTFSVNYGEIHALSGENG
                     AGKTTLMKILFGEEEATDGEIYIDGEKVRITSPLMAIKMGIGLVHQHFMLVPSLTVAE
                     NMVLGIEPRHGLSIDVDETERLVKEASQKFNFSIDPNKKIRDLTVGQKQKVEILKALI
                     RGAKILILDEPTAVLTPQETKELFIELKVLKEKGYTIIFISHKLNEVKELCDRITIIR
                     HGRTMGVYNLSEISEQEISRLMVGRDVALEINKKPPKPGKASIKVRDLKVIEESGKYS
                     LKGISLTAHYGEIFGVAGVEGNGQLQLAEALTGLGSYQSGTIELDGKEISRFSIGDIR
                     SIGVSHIPEDRMISGVAPHLSVAENVIADKLNFERFVKNGFLRSQSIRKFVIDIIKRY
                     KIACQSPDVSVDSLSGGNIQKVVLARELSSEPKIIIAEQPTRGVDVGATEFIRNELLA
                     SRDAGNTVFLISSDLNEILGLSDSLIVLYEGEIVAFFPDASKINEEDLGRYMLGIERQ
                     SFEQISEAYSE"
     gene            5609..6706
                     /locus_tag="PK898_08760"
     CDS             5609..6706
                     /locus_tag="PK898_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20161.1"
                     /translation="MNNKRINLLVDTVRMILIIAIAVALVFLIVFLTSKDPLNAIYSF
                     FIGPFTSVRRIGNIVEAASPLMFTALAVIIIFRSGLFSMISEGSFFIGILGAMIIGIS
                     CPLPAVIHPIVAILVAGLCGAVVASIPAALKMKWDVSEVVTSIMLNYVVQFFAIYMVS
                     YHFRETQSSSLASLLLRDTSKLSVLIKGTRVHSGIIIAFALCIICYIFLQHTRLGFKI
                     RIVGDNAYFAKNIGINVRTIMFLAQIFAGFIAGVGGGAELLGMYTRFKWTATPGYGWT
                     GIVVALLARRNPILVPLGALFIGYMNVGADIMSRSSDVSREVVYIIQGVMMLLIASES
                     LLQDWRQSLIVKEAKKEEMQHLETGNGKEII"
     gene            6708..7634
                     /locus_tag="PK898_08765"
     CDS             6708..7634
                     /locus_tag="PK898_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20162.1"
                     /translation="MSSLLSYILSADFLYMWIRVATPILFPALGAVICERAGVVNLGL
                     EGIMLISALFGVLGSYFFDGLIPGMLVGILASVVISCIFAYFHLVLHADNVLCGTAIN
                     TLASGLTVFLLELVTGEKGTSASVKSYSFPTVNLPYIQDIPVVGHILSGHNLLTYLAI
                     VAVFLVWFFVYKTPVGLRIRAVGENPNAASSVGISVIKMRFLAIILCGVLAGFGGMYL
                     SMGYLSMFTRDMTAGRGFIALAACAMGHATPVGAFLSALVFAFFDGLSNILQVLRMPA
                     EFVQMLPYVATILGLTLFSIQQGMQKKHKNQS"
     gene            7677..8453
                     /locus_tag="PK898_08770"
     CDS             7677..8453
                     /locus_tag="PK898_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU20163.1"
                     /translation="MAILDKESKTFGRKQYHIQLSPGDVGEYVLLPGDPARSDRVAGY
                     LENAKLVGNNREHRTFTGFYKGIKVSVTSTGMGCPSTSIAVEELINIGAECLIRIGST
                     GALQPDLKIGDLLISTGSMKNEGTSRFYVPDCFPAVPDFDLTRTLIDTALEMKENLGC
                     NVYYGICATDDAFYGETPEWIENLSKLGCLNVEMESSALFTVCHKRKKRGAMISAVSG
                     NLVNADVVYETENTGLVEGWNREIQVVLEAIYRFEQTKKI"
     gene            8455..9786
                     /locus_tag="PK898_08775"
     CDS             8455..9786
                     /locus_tag="PK898_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU20164.1"
                     /translation="MENTGVITLIHGHILTMNSTSDYYSDGMIVIRNKRIDYVGPFDP
                     MKVSGNVFDATDKLILPGLINTHAHSGSALFRSLADDLFLMDWLENYMWPAERFQTKE
                     SAYIAAALTHLEFLQNGITTNADMWYFPESVAQAVLESGLRTFICSTIFTKGSPESKD
                     TLGVAADFVEKYKGRSEETRIYPAYGPHAIYTCSQETLREVAELAKRDHVLIHTHISE
                     TSGEQKTCLERLGMTPSQAMKDVGIFENHVLAAHCIYLSEEDCEIFRKSQAAISYNPV
                     SNLKLCSGILPLEKMLDNDICVSIGTDGPQSNNSLDLLRDLKMGSLIQKNELKNPRFL
                     PAEKALRMATIDGAKALGMEKEIGSLEAGKRADIITLDTNRAVMIPLLDERPASLYSQ
                     VVYAASGSNVCDVFVDGECLMKNRQELRVDRQYISKTAQEIAKSSIKKNGL"
BASE COUNT         2940 a         1765 c         2363 g         2959 t
ORIGIN      
        1 gaaacagtcg atcttgtcta ttttttgata tcgccgatag gaacggtagt atttttatct
       61 aaaattctta aaagaaatat ggttttatac atattttttg aaatcatcct gaaaacagaa
      121 tcggaataaa gcgatataac acttcttttt ttgttgattt gattaaaaaa ccaacgggaa
      181 taatgtcata gttgaatcga tcaaagaaat gacaaaagga tattgacaac atggctgcca
      241 tgtcatatac tgtttttata acttggcagc catgtcattt ttaaatacaa aaaaattctt
      301 tttagaaaaa gcataaataa aaagggttga tttaccctaa aatacggagc acaaacataa
      361 tggctgatga ctataaaatg atgcacctcg gtattaaaaa aggagatgtc ggaagatatg
      421 tttttcttcc aggaagtgtc gaaagagcaa cattaatttc tgagtatttt gatcatccca
      481 ggaaaatcgc acataatcgt gaatttttga catattcggg gacattagat ggggttttgg
      541 tttctgtcac ttctaccgga atcggtggac catccaccac cattgccata gaagaacttt
      601 ttcaatgcgg cgccgatacc atgattcgag taggctcctg tgcctctacc tctccgattg
      661 caagaatcgg ggatgttatg attccaaacg gatccgttcg gatggaaggt accggtgttc
      721 attatttacc gatggaattt ccggcagttc ccgatttttt ccttttaaaa gaattggaac
      781 aagccgctat tcgattaaat ttccgatata acgtcggaat aaatatttgt aaagattctt
      841 tttatacgga agtttctccg gagacaaagc ccgtttatta tgaattgaaa gcaaaatggg
      901 aagcttatga aaagggggga gccacccata cttgtatgga atgcgctcct ctgtttttag
      961 cgggtgcctc acttggaatc cgaaccgctg cggttttgat ttgtgcgaca aattataaaa
     1021 gttattcaaa tgatgatgcg gattatccgc gagggtttga acataatgcg ataaaaaccg
     1081 gaatcgaagc gatgcgttcc gttattcata aagaccggca aaaagaacag gccaattaac
     1141 gagaggataa aaatggatga ctataaaatg atgcatctcg gtataaaaaa gggagaggtt
     1201 ggcgcttatg tattcctccc cggcagtccc gaaagatcag agaagatttc aaagtatttt
     1261 gaacatcctg taaaagttgc atacaatcga gaatttctca cctataccgg aactttggat
     1321 ggaatcccgg tatcggtgac atccaccggc atcggcggac cttcgaccgc tattgccgtt
     1381 gaagagttat ttagctgcgg agctcatacg atgattcggg tcggtacttg tgcttcaact
     1441 tcaccgaaag tgggaattgg ggatattgtt attcccaatg gcgcagtaag gatggaagga
     1501 gtgggtttgc actatcttcc catagaattt ccggctgttc cggattattt tctggtaaaa
     1561 gagctggaat cggcggcaat taaactggga tataaataca atatcggcat aacaatttcg
     1621 aaagccagtt tttataccca aacgaatccg gaaacaaaac cggtagcaaa tgaacttatt
     1681 gaaaaatgga gcgcttatga agccggcggt gctaccagca ccgagatgga aagtgcaccg
     1741 ctttttctgg tcggatcaag tttgaaaatt agaaccgctg ctgttttagt cagtgcaacc
     1801 aatttccacg aatattcgaa tgataaacga aactatccgg gtgatttaga gcatcgagca
     1861 atcgaaacag gtattgaagc gatgcgtgct gtcatccgaa aggatatgga ccgtgagtaa
     1921 tttagaggaa aagacagaat tgtctgttga aaagcagctg gacggattcg gaaaaattga
     1981 actttccaat attccccaaa tagatcgaac tgtcttggat ggtgttccca tttatgttcg
     2041 tatctgcgat attttttatc atcttgttga aaaaggcgtt gccaatgccg gggatcagct
     2101 tccgggagaa aatttattgg cggaacactg gaatgtcagt cgagcaactg tcaggcaggc
     2161 atttcggcat ttagaggagg atgggctggt ttacaagaga cagggaaaag gtacggtcat
     2221 tgctttcaat gcttccagtt tgcatcactc actcccttgg tgctacaaca tttgtctgga
     2281 aaattgtatc gaacaaatcg attcggtaga aatgaaatcg aatgttgaaa tgtgtggtga
     2341 atatctcgcg gaccgattgg aagcaactcc cgggaaggaa ttgcaaatca tggattttta
     2401 ttatcgatct caatatgaga tgatgagcac ttccgttttg atggtaatgg ttgaaaaatt
     2461 aaaatatttt gggattaatc ccgaaaatcg agatgacctg tatcgttttg gacaagaagg
     2521 aatttattcg tttgctgcga cggctcaatc atttatgacg gcattcgggg caaatgctga
     2581 taagaatatg aaaatgactg ttcccaaaaa tgatgtcgtt cttttcttag aagagattat
     2641 gcgggatgaa aaagaccaac ctatcggata cgcaagatat tatcttagga gcagttgtta
     2701 tcgaattccg atcaatagac gagccgtaat gtcatattaa ttatttattt gtcagaagcg
     2761 gggaaaacct gacccgtttg aagaaaaagg aggaattatg agtaaaaaat tgttttcagt
     2821 aatgttggca attgccgtgg tattttccat attcggaatt gtcgcggcag aagacgcacc
     2881 gatcaaggtc gttttgctta ttaatggcag tttgggtgat aagggctttt atgattcggc
     2941 agccagcggt gtccggcgaa tggaaaaaga actcggcgcc caaacgaaaa tcatcgaaat
     3001 gggacgcgat gaaactgctt atgagggcca ttatttggat gtgtctgaac aagattggga
     3061 tctgattatt tccggtacat ggtctgtgaa agagcttgca cagaatatcg ctgttcaatt
     3121 ccccgaaaaa cattatattt tctttgacgg taatgtggat cggacagttg tcacgacggg
     3181 aaatatgatg ggaatttcct atcttgataa tgaaggatct ttcatggcgg gtgcactggc
     3241 agctttgatg cttgattcag gtgccgaaaa aatcgatccc gataaacgaa tactcggttt
     3301 cgtcggttcg atggacaccg caaatattaa taacttcctg gttgggtata tcgaaggaat
     3361 caaatatatt gatccgagca ttaaattgct tacttcctac gtcggatctt ttgaggatgt
     3421 cccgaaagcc ctcgaaatga ccacccaact ttataaccag ggagcacaga tcgtatttgc
     3481 gccggcttct caaagcatcc tgggtgctgt aactgcttca agcaatgttg ataaatattt
     3541 tatcggttgc gatatggata cttgggcaca acttcaggat acagatgcga atctggccag
     3601 aaacattatt tcttccgctt tgaaaaatgt gggagattcg ctcgttacag cggttaaagg
     3661 ttatatggac gggacgatgg atacgagtca tgattattct ctcggtctga gcacaggtgc
     3721 tgtcggttta gcaaagaacg ataattacaa agcactggta ccggaagaaa ttcagaaaaa
     3781 actggatgaa attgagaaga aaattattga tggcgaaatc gtcgtcggtt cagccttcgg
     3841 aatgaaaagt gaagatgtcg cggctcttcg tgatgcgatg aaaccgtaac aaccattcga
     3901 aacatgagag tatggattta tttccatact ctcatgtttt atttcattta aattttgaat
     3961 taatgaggaa agatgaatat gaagtatcga atgccggaga gattactgtt tgttgttctc
     4021 caacgtcata tttcaaaggt taaaaagagg tagtcgatgg aaaaaacagc tgaggcatta
     4081 aggctgcaaa atatcaccaa agtatatccg aatggcgtca ttgcaaataa tggtgtgaca
     4141 tttagtgtta actatggtga aatccatgcg ctttcgggtg aaaacggcgc tgggaaaacg
     4201 acgctgatga aaattctttt cggcgaagaa gaagctaccg atggtgagat ctatattgac
     4261 ggagaaaaag tccggataac atccccgctg atggcgatca aaatgggaat cggcttggtt
     4321 catcaacatt ttatgttggt tccatcatta accgtggcag aaaatatggt attgggaata
     4381 gaaccccgac atggtttaag tatcgatgtc gatgaaacag aaagattggt taaagaagct
     4441 tctcaaaaat ttaatttttc gattgatcct aacaaaaaaa ttcgcgattt aaccgtcggt
     4501 caaaaacaaa aagttgaaat attaaaagct cttatccgtg gggcaaaaat ccttatcttg
     4561 gatgaaccta cagctgtttt gacacctcag gaaacgaaag agctttttat cgaattgaaa
     4621 gtattgaaag aaaaaggata tacgattatt tttatatccc ataaattgaa cgaagtaaaa
     4681 gaattgtgcg atcgaattac aatcattcgt catggtcgga caatgggagt ctacaactta
     4741 tccgagatca gtgaacaaga aatttcccga ttgatggttg gaagagatgt ggcattagaa
     4801 attaataaaa agcccccgaa accgggaaaa gcatctatta aggtgcgcga cttgaaggtg
     4861 attgaagaat ccggaaaata ttcgctgaag ggaatttcat taacagccca ttacggagag
     4921 atattcggtg tagccggagt agaaggtaat gggcagcttc aattggcgga agcgctgacg
     4981 ggattgggat cttatcagag tggcacaatt gaattggacg gaaaagaaat cagtcgattt
     5041 tcgatcggcg atatccgttc catcggtgta tctcatattc cggaagatcg aatgatatcc
     5101 ggcgttgctc ctcatttatc tgtcgcggaa aatgtaatcg cggataaatt aaatttcgaa
     5161 cgatttgtaa aaaacggatt cttaagatca cagtcgattc gaaaatttgt catcgatatc
     5221 ataaaacgat acaaaatcgc atgccagtcg cccgatgtga gtgttgacag cctttccggc
     5281 ggaaatatcc agaaagtggt tttagccaga gaactttcca gtgaaccgaa aattattatc
     5341 gccgagcaac cgacgagagg tgttgacgtc ggcgcaaccg aatttattcg gaatgagtta
     5401 ttggcttcca gagatgccgg aaatacggtt tttttgatct cttcagattt gaatgagata
     5461 ctggggcttt ctgattcatt gatcgttctt tatgaaggag aaattgttgc ctttttcccc
     5521 gatgcttcaa aaataaatga agaagatctc ggaagatata tgttgggaat tgaaagacaa
     5581 agctttgagc aaataagcga ggcgtatagt gaataataag agaatcaatc tattggttga
     5641 taccgtcagg atgattttaa ttattgccat tgcggtagcg cttgtctttc tgatcgtttt
     5701 ccttaccagc aaagatcctt taaacgcgat ttacagtttc tttatcggtc cgtttacttc
     5761 cgttcgaaga atcgggaata tcgttgaagc tgcttcccct ttaatgttta ccgctttggc
     5821 ggtcattatt atttttcggt ccggcttatt cagcatgatt tcagaaggat cctttttcat
     5881 agggatctta ggggccatga ttatcggaat ttcatgtcct ttacccgccg ttattcatcc
     5941 gatcgttgca attttggtag caggattatg cggagctgtt gttgcatcca ttcctgcggc
     6001 attgaagatg aaatgggacg tgagcgaagt cgtcacttcg attatgttga attatgtagt
     6061 ccaatttttt gccatttata tggttagtta ccattttcga gaaacgcaaa gttccagctt
     6121 agcttctctg cttttacgcg atacatcaaa attatccgtc cttattaaag gaacacgcgt
     6181 tcattctgga atcattattg cttttgcttt atgtattatt tgttatatct ttttacaaca
     6241 tacccgactc ggtttcaaaa tacgaattgt cggtgataat gcctatttcg caaaaaatat
     6301 cggaatcaat gttcgaacga ttatgtttct cgctcagata tttgccggtt ttatagccgg
     6361 tgtcggtggc ggagcggagt tgttgggaat gtacacacga tttaaatgga ctgcgacacc
     6421 cgggtacgga tggaccggaa tcgttgtggc gttgctggca cgaaggaatc cgattttggt
     6481 tccgctcggg gcattattta tcggttatat gaatgtcgga gctgatatta tgtctcgaag
     6541 cagtgatgta agtcgtgaag tagtgtacat tatccaagga gtcatgatgt tattaatcgc
     6601 ttccgaatcc ttgctgcaag attggcgaca atcacttatc gtaaaagaag cgaaaaaaga
     6661 agaaatgcaa caccttgaga cgggaaacgg aaaggaaata atctaagatg agttcactgc
     6721 taagttatat tctcagtgcg gatttcttat atatgtggat tcgtgttgcg actccgatat
     6781 tatttcctgc gttgggtgcc gttatttgcg agcgtgcggg agtcgtaaat ttagggctgg
     6841 aaggcatcat gctgatttcc gcattattcg gtgtgttggg atcttatttt tttgacgggt
     6901 tgatccccgg tatgttggtg ggaattctgg caagtgttgt gatcagttgt attttcgcat
     6961 attttcattt ggttttgcat gcggacaatg tattatgcgg gacagcaatt aacaccttgg
     7021 catccggctt gacggtattt ctgttggaac tggtaacggg tgaaaaagga acaagcgcgt
     7081 cggtcaaaag ttattctttc ccgactgtta atttgcctta cattcaagat attccggtgg
     7141 tgggccatat cctgtcggga cacaatttat taacctatct cgcaattgtt gcggtatttc
     7201 tcgtttggtt ttttgtatat aaaaccccgg tcggactgag aattcgtgca gtcggtgaaa
     7261 atccgaatgc tgcttcgagc gtcggcatca gtgtaataaa aatgcggttt ttagcaataa
     7321 ttttatgcgg ggttttggcc ggtttcggag gaatgtatct ttcgatggga tatctttcta
     7381 tgtttacgcg tgatatgacc gccggaagag gttttatcgc attggcagcc tgtgcaatgg
     7441 ggcatgcaac gccggtcggt gcctttttgt cggcattggt ttttgcattt ttcgatgggc
     7501 tttcaaatat actgcaagtt ttaagaatgc cggctgaatt tgtacagatg cttccatacg
     7561 ttgcgacgat attaggtttg actttgtttt cgattcagca agggatgcaa aagaagcata
     7621 aaaatcaatc ctaagaatta aagaacattc agaaaacctg aagataagga gatatcatgg
     7681 ctatattaga taaagaatca aagacctttg gaagaaagca atatcatatc cagctttctc
     7741 cgggagatgt cggggaatat gttcttctgc cgggggatcc ggccagaagt gatcgcgtag
     7801 cgggatattt ggagaatgcc aaactggttg gcaataatcg ggagcatcgg acttttaccg
     7861 gcttctataa aggaattaaa gtcagtgtta cttcaaccgg aatgggatgt ccatccacct
     7921 caatcgctgt ggaagaatta ataaatatcg gtgccgaatg tttaatccgc atcggcagta
     7981 ccggggcgtt gcagcctgat ttaaaaatcg gtgatttact gatttcgacg ggttcgatga
     8041 aaaacgaagg tacctctcgt ttttatgttc cggattgttt tccggcagtt ccggattttg
     8101 atctgacacg aacattgatc gatacggcgt tggaaatgaa ggagaatttg ggatgtaatg
     8161 tatattatgg gatttgtgca accgatgatg ccttttatgg cgagactccc gaatggattg
     8221 aaaacctttc caaattagga tgtctgaatg tggaaatgga aagttcggca ctttttactg
     8281 tttgccataa acgtaagaaa cgaggcgcaa tgatctctgc tgtttcagga aatcttgtca
     8341 atgcggatgt ggtatatgaa acagaaaata ccggattggt tgaaggctgg aaccgtgaaa
     8401 ttcaggttgt attagaagct atttatcgtt ttgagcagac gaagaaaata taggatggaa
     8461 aataccggag ttattacact tattcacggt cacatcctta cgatgaattc aacgagtgac
     8521 tattacagcg atgggatgat tgtgattcgc aataagcgga tcgattatgt cggccctttt
     8581 gatccgatga aagtgagcgg aaatgtcttt gacgctacgg ataagttgat attgccgggg
     8641 ttgattaata cacatgccca ttcaggcagc gccttgtttc gcagcttagc cgatgatctt
     8701 tttttgatgg attggttgga aaattatatg tggccggctg agaggtttca gaccaaggag
     8761 tcagcctata ttgcggctgc attgacgcat cttgaatttc tgcaaaacgg gatcacaacc
     8821 aatgcggata tgtggtattt tcccgaaagt gttgcacaag cggttttaga aagcggattg
     8881 agaacattta tctgttccac gatatttaca aaaggttctc cggaatcgaa agatactctg
     8941 ggagttgctg ctgattttgt agaaaaatac aaaggtcgct ctgaagaaac acgcatttat
     9001 ccggcttacg gtccgcatgc catttacacc tgttcccaag aaacacttcg tgaggttgcg
     9061 gagttggcaa aacgggatca tgtgctgatt catactcata tttccgaaac gtcaggtgaa
     9121 cagaaaacgt gtcttgaacg ccttggcatg acaccttcac aggctatgaa agatgtaggt
     9181 atttttgaga atcatgtttt agcagctcat tgcatttatc tatccgaaga agattgtgag
     9241 atttttcgaa aaagtcaagc agcaatcagc tacaatcccg tcagtaatct aaaactttgt
     9301 tccggaattt tgccccttga aaaaatgtta gataatgata tctgtgtatc gatcggaacc
     9361 gatggtccgc aaagcaataa cagccttgat ctgttgcgtg atcttaaaat gggttcgttg
     9421 atccaaaaga atgaattgaa gaatcccaga tttcttccgg ctgaaaaagc tctgcggatg
     9481 gcgacgattg acggtgcaaa ggcattaggg atggaaaaag aaatcggcag cttggaagca
     9541 gggaaaagag cggatattat taccctggat acaaatcgag cagttatgat tccgcttctg
     9601 gatgaaaggc cggcaagtct ttattcgcag gtggtatatg ccgcatcagg ttcaaatgtc
     9661 tgtgatgttt ttgttgatgg cgaatgtctt atgaaaaaca ggcaagagct gagagttgat
     9721 cgtcaatata tttcaaaaac agctcaggaa attgcgaaaa gcagcataaa gaaaaacgga
     9781 ttataacgat tcgtaaaaat tctcatttct attcctttct ttcatttgaa gaaatctcaa
     9841 aggtatcctt tgagatttct tcgatcattg atgaaacatc agagtcaatt atcgacaaag
     9901 agttcgatta ttggtaaatt attatctaag gaatttttat agtatgatcg gaaaataaat
     9961 ttatttcttt ttgggaacgg atatgaatgg tagagcgtat ttctctcttt ttgccctttt
    10021 atctcgg
//
LOCUS       DAOSMM010000070         9887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_6827_length_9887_cov_17.247457, whole genome shotgun sequence.
ACCESSION   DAOSMM010000070 DAOSMM010000000
VERSION     DAOSMM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6827_length_9887_cov_17.247457"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1271)
                     /locus_tag="PK898_08780"
     CDS             complement(54..1271)
                     /locus_tag="PK898_08780"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOU20165.1"
                     /translation="MVREQFQVKINSFTLNVTQTAVDAVRKKQITKTGCRVYSDGYIG
                     ISGTFGEADEQTWLDAEKNLMKKIPYPYPPSAGKTRIRNTKKACGSQTDFLKQSEEFL
                     ARIRKDFPDFICSNKLMSVESEVSIHNDLGLDYCNSDQSIVFGLVVKHIKSINVFDTI
                     YSFEDRDPDFESVYEDLSSQLTAFRNPMEQKISGKIPVVIGFQDISGKFIESLNGEAL
                     GRGVSIFGDKIGKKVFNDDFSLIVDRGIEPIHASFFDVEGSVLESDQTALIEKGQIRC
                     GYADKKTADKYGMRNTAAAGGEYNSVPALSVPPLSVLPSQKTLAEILNGRDAIFAAVL
                     SGGDFTNEGQFASPVQMAYSIRDGKLVGKLPELTISGNIYDLFGNDYFGQSGDKPFCG
                     ERYLVLNMDVNQY"
     gene            complement(1284..2621)
                     /locus_tag="PK898_08785"
     CDS             complement(1284..2621)
                     /locus_tag="PK898_08785"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU20166.1"
                     /translation="MYQYPKNLYTDVRIETVSKTNIQFENGELKENKTVSEKGAMVRI
                     YDGKRWYYSATTDLNSIQNEIDDLAEMAVPNSAILQDSVVDKLEINRGMDLRYQQDDV
                     SLISNDKKMALVKSYLELISDYPEIRNSKIFYLDNHTVKHFVSGKGADITFDTQNCTI
                     VVRYTIMANDFPQDGQANIYKIRFHELAGHQREVRDEIEKDLDYAKRAVPVKPGVYTC
                     ILSPLVAGVFAHESFGHKSEADFMIGDEEMRKEWVIGKKVGASLLNIIDSGQIEGSGY
                     VPYDDEGCRAKKTYLIKDGVLSGRLHSAYTAALLNENPTANARAISFEYEPIVRMTTT
                     YIDSGKQTKEELFGEVEDGIFIDDFKHGSGMSTFTIAPSRAYRICHGKIAEPLKIAVI
                     TGNVMKTLHEIDGVSDAVEIKSFGTGGCGKMEQYPLRVGFGGPYVRVNGINVQ"
     gene            complement(2728..3492)
                     /locus_tag="PK898_08790"
     CDS             complement(2728..3492)
                     /locus_tag="PK898_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU20167.1"
                     /translation="MKDSYTIGEFSKMFGLNVQTLRYYDSIGIFRPAYRDKWSGRRKY
                     AFDQVYQLASIRYLRRIGYSLEDIAEYLDNLQSDTSLDVLKKRSDEIHRELDELLDID
                     HAIQRKIRFIEHELSEIREISEIKICEYGPRPYIPLGQEEVLYHHDSFYFYPTIAFYK
                     EDLKYFGAYLYQDNSQFPEDFKVNKEKVEYIEAGKYLCAYHLGPYEEIYKTQKKLRNA
                     YSNLKLSDLMVNFNIIDQFVEKDRSKYITSMQIRIL"
     gene            complement(3535..4470)
                     /locus_tag="PK898_08795"
     CDS             complement(3535..4470)
                     /locus_tag="PK898_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20168.1"
                     /translation="MSQILLTIATFLAACIRMATPLTLAGLGEAFSERTGVMNLGVEA
                     IMLCGAFSSFIVTFWTGNVYWGMLCGMLAGMIISGIHAILCIKCKANQTIVGLALNFF
                     ALGLTSFLFLIVFGQSTILPQCKTVPNFEFPVLGKIPLLGKAFFTHNIFVYLMYLLVF
                     IFLFIYKKTEWGVIFTAVGEHPSAADTAGINVFRIRYLACMVNGLLGGLAGSYFTLAQ
                     LGFFMEDITAGKGYIALVTVILGRRNPLGILCSALLIGLADAVQFQLQTMNIPIPSQA
                     FSILPYIAAVFVLLFSAGKSSDPSALGIPYERDNR"
     gene            complement(4467..5504)
                     /locus_tag="PK898_08800"
     CDS             complement(4467..5504)
                     /locus_tag="PK898_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20169.1"
                     /translation="MKNKAGDLISYLMIVLAGIILMILLIGITGNSISDALSGFIRGI
                     AGSSYSVAEVFVKATPLILTGLGVSVAFKSGFVNIGAEGQLYMGAVTITALALAFPNL
                     PKGILMPLCLLGGFLAGGVWSLIPGILKAKMGISEVINTIMFNYIALGIVGILLQTVL
                     KDPNNYFPVSATLTDSAGLSILIPGTRLHSGILIAVICAILIDILIFKTSLGFRLRAV
                     GMNPRACLCSGISVGKHIIISSMISGGLAGIAGVCEIAGLHHKLIEGISPGYGYLAII
                     VALLGGNNPFGVILAAIGIATLQVGSLSMQRAANVPSAISSIILGAIVLMLMARKTIF
                     KKLIDKKEEQG"
     gene            complement(5494..7053)
                     /locus_tag="PK898_08805"
     CDS             complement(5494..7053)
                     /locus_tag="PK898_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20170.1"
                     /translation="MTVKVEEKMILLSMENIQKKFFGKYANKNVHLQVEKGKILALLG
                     ENGAGKTTLMNILYGLYQPDGGKIFWHGEEVKFESPKDAIERKIGMVHQHFMLVPTLN
                     VSQNITLGLKETGYPLTDRKAVNRKIKSLSDQYGLIVDPEAMVSDLSVGEQQRVEIMK
                     LLYRDAELLILDEPTAVLTRQETIGLFEVLKRLSQDGRSVILITHKIPEVMEIADMVT
                     VMRDAESIITVPITEVDEASLAEYMIGRPLNIVEIPPRIEKRNQGFILRNLNLEEKSQ
                     TKSATVSFEIEAGEILGIAGVEGNGQKELAESILGIRHNVAEEMQLFGKDLRNHSVSD
                     RRDAGIAYISDDRHRDGLLLDAGFMDNMLLKFHKDRRYQNNGILNKKKLNDFINRQIK
                     AYKIKVSSPSLPVRFLSGGNQQKLILSRELCDEPKLIVACQPTRGLDIGATEFVREQL
                     LMHRSKGACILLISADLEEILSLSDRIAVLYKGRIMGILNNSKNIDITRIGLMMAGRN
                     FNLNEVEIHEK"
     gene            complement(7095..8210)
                     /locus_tag="PK898_08810"
     CDS             complement(7095..8210)
                     /locus_tag="PK898_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU20171.1"
                     /translation="MKKSLSILLFVVLIGSMLISGCSSGAKSTPTTAAVLTSTETTEV
                     KSEKEATSSVIPSAESKTVETGSGAKKLKVALILSGPANDQGWNATALEGLQAAEKEF
                     GVETSKMENVDIADSEAAFRDYAAQGFDLIIGHGYQFGEPAKKVSADFPNSYFVATEA
                     NSQSENMASYVMSCEQAAYLMGMLCAGMSKTKVIGVVGGFEQPSITKELEAFKLGAKE
                     VVPDIKVLEVYINSFVDASLGKEAAMSMIRQNADVLYHVANQAGTGVIMAAQENGLLA
                     CGNSYDQSSIAPDTVMASTVYRMPKVITTAVNQVKTGTFKGGVYHLGFADDVVDITGY
                     GKFEDKIPQDLKDKIAAKKEAIRTGEFVVPVIEKPTK"
     gene            complement(8235..9629)
                     /locus_tag="PK898_08815"
     CDS             complement(8235..9629)
                     /locus_tag="PK898_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU20172.1"
                     /translation="MDEKILIRNAESIVSCDENDTVYHDADILIENGFIKEIGKNIST
                     DDSKIIHAEHKIIYPGLINTHHHFFQTFVRNLITIDYPNMLVVEWLDKIYRIFQMINS
                     DVIYYSSLTAMADLLKHGCTCAFDHQYCYTKATGKSPVDRQMEAAELLGIRYHAGRGT
                     NTLPRSEGSTIPENMLETTDEYLKDCERIIDLYHDPQPGSMHQIVLAPCQPINSYKET
                     FTETIALAREKGVRMHTHLGEGENEIMLQRWGKRSLAWCEEIGFVGPDVWYAHFWEGL
                     PEEDAILAKYGCGVSHCPAPAILGGFPILDIKRMQQSGITISLGCDGSATNDSSNLLD
                     SMRIGYLMQTWHSKQRNGCISAYDMLKIATVSGAKTLGRSDLGSLEKGKCADLFMVDT
                     DVPELIGTNHDHKNILARVGLTGPVWLTMVAGKIVFEEGHLVGVDESKLARRGEEVCD
                     RVIREPFKEFFIVS"
BASE COUNT         2819 a         2335 c         1798 g         2935 t
ORIGIN      
        1 caaaaaacag gctaagcact ttaacctgtt tttatatttt cggttcattt ttattaatat
       61 tgattaacat ccatatttaa taccagataa cgttcgccgc aaaaaggttt atcaccactt
      121 tggccgaaat aatcatttcc gaataaatca tagatattcc cggaaattgt taattcgggt
      181 aatttcccga ctaatttacc gtcccgaatg gaatatgcca tttggacagg tgaagcaaat
      241 tgtccttcgt tggtaaagtc tcctccgctt aaaaccgctg caaagatggc gtctcgacca
      301 ttcaatatct cagccaaagt tttctgtgac ggtaaaaccg aaagaggggg tacactcaat
      361 gccgggacac tgttatattc gccccccgcc gcagctgtat tccgcattcc gtatttatcg
      421 gcggtctttt tatcggcata tccacatcgt atttgtccct tctcaatcag agccgtttga
      481 tccgactcaa ggacgctacc ttcaacatca aaaaacgaag catggatcgg ttcaattccc
      541 cggtcgacta ttaaagagaa atcatcgtta aaaacctttt tcccgatttt atcgccaaat
      601 atggatactc ctcttccgag tgcctcaccg ttgagtgatt cgataaattt accgctgatg
      661 tcttgaaaac cgattacaac cggaattttt ccgctgattt tttgctccat cggattccta
      721 aaagcggtca attgagaaga tagatcttca tagacggatt caaaatcggg gtcgcgatct
      781 tcaaacgaat aaatcgtatc aaatacatta atcgatttta tatgttttac caccaagccg
      841 aaaacaatcg attgatccga attgcaataa tccaatccga gatcattatg aattgagact
      901 tcggattcaa ccgacattaa cttattactg cagataaaat ccggaaaatc ctttcgtatt
      961 cgcgctaaaa attcttcact ctgtttgaga aaatccgtct gagaaccgca agcttttttg
     1021 gtatttcgaa ttcgtgtctt tccggctgag ggaggatatg gataagggat tttcttcatc
     1081 aagtttttct cggcatcaag ccaagtttgc tcatcggctt ccccgaaagt acccgagata
     1141 ccaatgtaac cgtccgaata gactctgcaa ccggtttttg taatttgttt ctttcgcacc
     1201 gcatccacag cggtttgagt gacatttaag gtaaaactgt tgattttcac ctgaaattgt
     1261 tcgcgtacca tatcttttcc tttttactga acattaattc cgttgacccg gacatacggg
     1321 ccgccaaaac cgacccgtaa gggatattgt tccatcttgc cgcatccgcc ggttccgaat
     1381 gattttattt ccacggcatc ggaaaccccg tcaatttcat gaagtgtttt cattacattc
     1441 ccggtaatta ccgcaatttt aagtggttcg gcaatttttc catggcaaat tcgataagca
     1501 cgggaaggcg ctatcgtaaa agtagacatt ccggatccgt gcttaaagtc atcgataaaa
     1561 atcccgtctt ccacttcacc gaataattct tctttggttt gtttcccgga atcgatatag
     1621 gtggttgtca tacggacaat cggttcatat tcaaaactga ttgcccgagc attggcggtc
     1681 ggattttcat tcaataacgc agcggtatat gcgctgtgta atcgaccgct caacaccccg
     1741 tctttgatca ggtaagtttt cttcgcccga catccttcat cgtcataagg aacatatccg
     1801 gacccttcga tttgtccgga atcgataata tttaataacg aagccccgac ttttttaccg
     1861 ataacccatt ctttccgcat ttcttcatca ccgatcataa aatccgcttc gcttttatgt
     1921 ccgaaactct catgtgcaaa aacgccggca accaaagggg aaagaatgca agtatacaca
     1981 cccggtttaa ccggaacagc ccttttggca tagtccagat ccttttcaat ctcatctcgg
     2041 acctcacgct gatgcccggc caattcatga aaacggatct tatagatatt cgcctgccca
     2101 tcttgcggaa agtcgttcgc catgattgtg tatcggacga caatcgtgca attttgagta
     2161 tcaaatgtga tatcggcgcc cttaccggaa acaaaatgtt tcacggtatg gttatccaga
     2221 taaaagattt ttgaatttcg aatttcggga taatccgaga tcaattcaag ataacttttt
     2281 accaatgcca tttttttgtc gttggaaatc aaggaaacgt catcctgctg atagcgcaaa
     2341 tccattcccc gatttatttc aagcttatca accaccgaat cttgaaggat cgccgaattc
     2401 gggacagcca tttccgccag atcatctatt tcattttgaa tcgaattcaa gtcggttgtt
     2461 gcgctgtaat accagcgttt cccatcataa atccgaacca ttgccccttt ttcggatacg
     2521 gttttattct ctttcagctc tccgttttca aattgaatat tggtttttga aactgtttcg
     2581 attcgaacat cggtatataa attttttgga tattgataca tagagcattc tccttacgtc
     2641 aattatagcg agtttgaata caaactgtta tcgggaaatc agccggacgc ggttaccaag
     2701 tgaaattact aaaatgattt ttttgcgtca caaaatacga atttgcatgc tcgttatata
     2761 ctttgatcgg tccttttcga cgaactgatc gataatattg aaattaacca tcagatccga
     2821 aagttttaaa ttcgagtaag cgttgcgcaa ttttttttga gtcttgtaaa tctcttcata
     2881 cggtcccaaa tgataagcgc ataaatattt gcccgcttcg atgtattcaa ctttttcttt
     2941 attgacttta aaatcttcgg ggaattgact gttgtcttga tataaataag cgccaaaata
     3001 tttcagatct tctttataaa atgcaatggt cggataaaaa taaaaagaat cgtggtgata
     3061 caatacttct tcctgcccta aaggaatata aggtcggggt ccatattcac atatctttat
     3121 ttcagaaatt tctcgaattt cgctgagttc gtgttcaata aaacgaattt tgcgctgaat
     3181 cgcatggtca atgtcaagta attcatccag ttcgcgatgg atctcatccg atcttttttt
     3241 caaaacatct aatgaagtgt ccgattgcag attatccaaa tattcggcaa tatcctctaa
     3301 agaatagccg atccggcgta agtagcgaat cgaagccaat tgatagactt gatcaaacgc
     3361 atattttcgc cgacccgacc atttatccct atatgccggt ctgaaaattc ctatcgaatc
     3421 gtaataacga agtgtttgga cattcaatcc aaacatctta ctgaactctc caatcgtgta
     3481 gctgtctttc aaccggacac tcctccttga aaatcaattc cttctctcat tttatcagcg
     3541 gttatcgcgt tcatagggga tccctaaagc ggaaggatcg gaacttttcc ctgccgaaaa
     3601 gagaagaaca aaaacagctg cgatataagg caaaatggaa aaagcttgag aaggaatcgg
     3661 aatattcatc gtttggagtt ggaattgaac ggcatccgcg agcccgatca ataaagccga
     3721 acaaagtatt cccaacgggt ttcttctgcc taatataacc gtgaccaaag caatatagcc
     3781 ctttcctgcg gtaatgtcct ccataaaaaa tccgagctga gccaacgtaa aataggagcc
     3841 tgccagccct cctaataatc cattcaccat acaagccaaa taccgaattc gaaacacatt
     3901 gattcccgcc gtatcggcgg cactcggatg ttcaccgacc gctgtaaata taactcccca
     3961 ttccgtcttt ttataaataa aaaggaaaat gaaaaccaat aaatacatca ggtaaacgaa
     4021 aatattatgt gtaaagaaag ctttcccgag taacggtatt ttgccgagca ccggaaattc
     4081 aaaattggga accgttttac attgaggaag aatcgtgctt tgaccgaaaa cgattaaaaa
     4141 cagaaagctg gtcagcccca gtgcaaaaaa attcaaagca agacccacaa ttgtctggtt
     4201 tgctttgcat ttaatacaca gaatcgcatg aataccggaa ataatcatac cggcaagcat
     4261 tccgcacaac ataccccaat aaacatttcc ggtccaaaaa gtcactataa aagagctaaa
     4321 agctccgcac agcatgattg cttcgactcc caaattcatg acgccggtac gttctgaaaa
     4381 agcctccccc aaacccgcca aagtgagagg tgttgccata cgaatacaag cggcaagaaa
     4441 tgttgcgatg gttaataaaa tctggctcat ccttgctcct cttttttgtc tatgagcttc
     4501 ttaaatatcg tttttcgcgc catcagcatc agaacaatcg ctcctaaaat aattgaggaa
     4561 attgccgaag ggacatttgc cgcacgttgc atagaaagtg atccgacttg taacgtggca
     4621 attcctatag ctgccaatat tacaccaaac ggattgtttc ctcccaacaa tgccacgata
     4681 atcgctaaat aaccatatcc gggagaaatt ccctcaatca atttatgatg aagaccggcg
     4741 atttcgcata cacccgctat cccggctaaa ccgccgctga tcatagatga aataataata
     4801 tgtttgccca cagaaatacc ggaacaaaga catgcgcgcg gattcatacc caccgctcgc
     4861 aatcgaaaac cgagtgatgt tttgaaaatc aaaatatcga tcaggattgc acagataacg
     4921 gctattaata ttcccgaatg caatcgtgtt ccgggaatca aaatggataa accggccgaa
     4981 tcggtcaagg tcgccgagac aggaaaataa ttattcggat cttttagaac cgtttgcaat
     5041 aaaattccga caattcccaa agcgatatag ttaaacataa ttgtattaat gacttcggaa
     5101 attcccattt tcgcttttaa aataccgggt attaatgacc aaactcctcc ggctaaaaat
     5161 ccacccaata aacagagcgg catcaggatc cccttcggta aattgggaaa tgccaatgcc
     5221 agagccgtga tcgttaccgc tcccatataa agttgacctt cggcaccgat attcacaaag
     5281 ccgctcttaa acgcaacgga tactcccaag ccggttaata tcaacggagt tgctttcacg
     5341 aaaacttctg caacggaata ggaagagcct gctatccccc gaataaaacc ggataacgca
     5401 tccgaaatac tattgccggt tatcccgatt aagaggatca taagaatgat ccctgccaga
     5461 acaatcatca aataggatat aagatcaccg gctttatttt tcatggattt cgacctcatt
     5521 caaattaaaa ttcctgcctg ccatcattaa tccaatccgt gtgatatcaa tatttttact
     5581 attattcaga atacccatta ttctgccttt ataaagaact gcgattcgat cacttaacga
     5641 aagaatttct tccaaatcgg cagaaatgag taaaatacag gcacctttgc tacgatgcat
     5701 taataattgt tctctgacaa attccgtcgc cccgatatcg agacctcttg tcggttggca
     5761 agccacaatt aatttcggct cgtcgcaaag ctctctggaa agaatcaact tttgttggtt
     5821 tccgcctgat agaaatcgaa cgggcaggga aggagatgaa acttttattt tataagcttt
     5881 gatctgtcga ttgatgaagt cgtttaattt ctttttgttc aaaatcccgt tattctgata
     5941 tcttcggtct ttatggaatt tgagaagcat attatccata aaccctgcat ccaacaataa
     6001 accgtcgcga tgtcgatcat cggaaatata ggcgatgccg gcatctctgc ggtccgaaac
     6061 cgaatgattc cttaaatctt tcccgaaaag ctgcatttct tccgcgacat tgtgccgaat
     6121 tcccaatatc gattcggcca actctttctg tccattcccc tcgacaccgg caatccccaa
     6181 tatctcgccc gcttctattt caaacgaaac agtcgctgac tttgtttggc tcttttcctc
     6241 aagatttaag ttccgcagaa taaagccttg attacgtttt tcaattctcg gaggtatttc
     6301 aacaatattc agaggtcgcc cgatcatata ttcagctaaa cttgcttcgt cgacttctgt
     6361 tatcggaacg gtaataatac tttcagcatc cctcatcacc gtaaccatgt cggcgatttc
     6421 cattacttcc ggtattttat gagtgatcag aatgacggaa cgaccgtctt gagagagcct
     6481 ctttaatacc tcgaacaaac caattgtctc ttgtcgcgtc aaaaccgcgg tcggctcatc
     6541 caaaatcagc aactcggcat ctcgataaag caacttcatg atttcaacac gctgttgttc
     6601 gccgacggat aaatcgctga ccatcgcctc cggatcgacg attaatccgt actgatccga
     6661 taaacttttt atcttccgat taactgcttt ccgatcggtt aatggatagc cggtctcttt
     6721 taatccgaga gttatatttt gagatacatt gagagtgggc accagcataa aatgctggtg
     6781 caccattccg atttttcttt cgatcgcatc tttgggagat tcaaacttta cttcttcgcc
     6841 gtgccaaaat attttaccgc catccggttg gtaaagaccg tagagaatat tcatcaaggt
     6901 tgtctttccg gcgccatttt cgccgagcaa agcaagaatt ttcccctttt ctacctgaag
     6961 atgaacattt ttgttggcat attttccaaa gaattttttt tggatatttt ccatagaaag
     7021 caatatcatt ttttcctcga cttttactgt catctgttca ctcaggcaag aacaatttct
     7081 ttgaatctca acatttattt tgtgggtttt tcgataaccg gaaccacaaa ttcaccggtt
     7141 cgaatagctt cttttttcgc ggcgatttta tcttttaaat cctggggaat tttatcttcg
     7201 aatttaccgt aaccggttat atccaccaca tcatccgcga aacccaaatg atatactccg
     7261 cctttaaagg tccctgtttt tacttgattc acagcggtgg ttattacttt cggcattctg
     7321 taaaccgtcg atgccataac cgtatccgga gcaatactgc tttgatcata tgaattacca
     7381 caggccaaaa gaccgttttc ctgtgcagcc ataatcacac cggtgccggc ttggtttgca
     7441 acatgataca aaacatccgc attttggcgg atcatgctca tcgcagcttc ttttcccagc
     7501 gaagcatcga caaatgaatt gatgtaaact tcaagaactt tgatatcggg aacgacttcc
     7561 tttgcaccca atttaaacgc ttccaattcc ttggttatgg aaggttgctc gaaaccgccg
     7621 acgacaccga ttactttggt tttactcatc ccggcacaaa gcatgcccat taaatatgcc
     7681 gcttgttcac aactcatcac gtaagaagcc atgttttcgg attgtgaatt tgcttccgtg
     7741 gcgacaaaat aggaattcgg aaagtctgcg gatacttttt tggccggttc accaaattga
     7801 tagccgtgac cgatgatcaa atcaaacccc tgagcggcat aatctcgaaa agctgcttca
     7861 ctgtcagcta tatcgacatt ttccattttt gaggtttcaa ccccgaattc tttttcggct
     7921 gcctgcaagc cttccaatgc ggttgcattc caaccttggt catttgcggg accggacaga
     7981 attagtgcaa ctttcaattt cttcgcaccg cttcccgttt cgactgtttt gctctcagcg
     8041 gacgggataa cagatgacgt cgcttctttt tcggatttga cttcggtagt ctctgtcgaa
     8101 gttaggactg cggctgttgt cggtgtactc tttgctcccg aggaacaacc tgaaataagc
     8161 atactgccaa tcaatacgac aaataacaaa attgataaac ttttcttcat ttctccctct
     8221 catctaataa acaattacga aactataaag aattctttaa acggttcacg aattacccga
     8281 tcacatacct cctctcctct ccgcgcaagt tttgattcat ccacacccac cagatgacct
     8341 tcttcaaata caatctttcc ggctaccatg gtgagccata ccggtccggt caatccgacc
     8401 ctcgccaata tatttttatg gtcatgattt gtcccgatta attcggggac atcggtatca
     8461 accataaata aatcagcgca ttttcctttt tctaaactcc ctagatcgct tcgtccgagc
     8521 gttttagccc cggacaccgt cgcgattttc agcatgtcat aagctgaaat acaaccgttt
     8581 ctctgtttgc tgtgccaggt ctgcatcaaa tatccgattc gcatggaatc taataaattg
     8641 gaggaatcat ttgtcgcgga tccgtcacaa ccgaggctga ttgttattcc ggactgttgc
     8701 attcttttta tgtccaaaat cgggaagccg cctaaaatcg ccggcgccgg acaatgcgag
     8761 acaccgcatc cgtattttgc gagaatcgca tcttcttccg gtaacccttc ccaaaagtga
     8821 gcataccaaa catccgggcc gacaaatccg atttcctcgc accacgccaa agaacgtttc
     8881 ccccaacgtt gaagcatgat ctcattttcc ccttcaccca gatgagtatg catccgaacc
     8941 cctttttccc gtgccaaagc aatcgtctcg gtaaaagtct ccttataaga attgatcggc
     9001 tggcacggag ccaaaacaat ttggtgcatc gaacccggct gaggatcatg atataaatcg
     9061 atgattcgtt cacaatcttt taaatattca tctgttgttt ccaacatatt ttccggaatc
     9121 gtgctgcctt cgcttctcgg gagcgtattt gtccctcgtc cggcatgata acggattccc
     9181 aaaagttcgg ctgcttccat ctgtctgtcc acgggtgatt taccggtcgc ttttgtgtaa
     9241 caatactgat gatcgaatgc acaggtacaa ccgtgcttta acaaatcagc catagctgtc
     9301 aaagaactgt aatatatgac gtcggaatta atcatctgaa aaatgcggta aattttatcc
     9361 aaccactcta ccacaagcat attgggataa tcgatcgtaa ttaaatttcg aacaaatgtt
     9421 tgaaaaaaat gatgatgggt atttattaat ccgggataaa taattttgtg ttccgcatga
     9481 atgattttcg aatcgtctgt tgaaatattc tttccgattt cttttatgaa tccgttttcg
     9541 attaagatat ctgcatcatg ataaaccgta tcgttttcgt cacaggaaac aatcgactcg
     9601 gcattacgga tcaggatttt ttcatccata tgaatctcct cagtttttaa aatgtcattt
     9661 ttattcgcat gtaacttagt atttatgatt ctacaaccta tagttactat aggtgcaagg
     9721 attcatgtag ttcaacaaaa aactcttaag agttttaaca actcttaaga gttttcgtta
     9781 tgtcgtttca attcaattta tattgcgtga ttcaaagatt caattcgcga ttaaacaaaa
     9841 agaatcatgt tggcgataac ctactctccc aggaggtcgc cctccaa
//
LOCUS       DAOSMM010000071         9435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_7237_length_9435_cov_15.431237, whole genome shotgun sequence.
ACCESSION   DAOSMM010000071 DAOSMM010000000
VERSION     DAOSMM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7237_length_9435_cov_15.431237"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PK898_08820"
     CDS             complement(<1..869)
                     /locus_tag="PK898_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU20173.1"
                     /translation="MKKSVFRFIVLTVLIVSLVGVSTVFAENSIKGKVVAFIPKVTGN
                     SFFEAANAGAQKYAEKWGITVKYMGSATASVTDQLEVIQQAIDAGVDAIAISSVDAKA
                     LDEKLKEAQEQGIVVVTWDSDVSSDARSLMVSQGTASQLGPMLVEMGVESLKERGVDV
                     NGPVKYVWHYSQAAVADQNSWYVAGEAYIKEKYPNWVAVADPYYSEQDPQKAVAIGEA
                     ILDAHPDIDLIICNDSTALPGQCTAAENKGLTKDDITITGFCPPSGMTAHLQAGTIAR
                     WGLWDCGLQGALA"
     gene            complement(904..1908)
                     /locus_tag="PK898_08825"
     CDS             complement(904..1908)
                     /locus_tag="PK898_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20174.1"
                     /translation="MKNKLTNIFKNWEFTLLVILILEFVIFGSINPKFFRIASIFSNI
                     NNYISICIISLFVTFVMITGGIDIQASSIVGLTSITIGVLWNDAGLNIWMAVAIAVLL
                     AAFCGALSGFFIAYCHVQPMVVTLGGSFLYSGLAQVISNFSKIPAYQGISGFPGKNAN
                     GDYISFRFLGKAKLFDLIPIQIVIYLILIILCYVLLHQSKYGRRIFLVGDNPKAAEYS
                     GINSRLIIFSTYVLTGIGAAIAGILLTAQVDSAKYNLGSTFTLSIITAVVLGGTLTSG
                     GKGSVIGTALAALVICLLRFGLTLCFNISSQNLDLPVGIMLILVVSLRQINRIVHHLD
                     "
     gene            complement(1905..2933)
                     /locus_tag="PK898_08830"
     CDS             complement(1905..2933)
                     /locus_tag="PK898_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20175.1"
                     /translation="MNKIIKVLTESRWFSSLLFLIALFLITGAINPTFLSYGNIITCF
                     NSSIVFILLAVGISFVIMTGEIDVSIGSTMGLSAAIAGSIAQQNGSWVMMVVYAILLG
                     LAVGFVNGVGVAILKVPSLIFTLGTYGIVRGLVYIYSGGRTVENFSGAFTRFSNSTVG
                     NTGIGIYLLIVTVIVLIINVVETKTRQGKYFLAVGDNYDGAILVGIPAKRTKLIAYIL
                     CGIFAAISGIAFSSKYGQVTIIAGSGYEMSAIAACVLGGISLSGGLGNVIGAAIGAVI
                     MSSISRLLVFLGFSSEYDNTITGILLITIVVLDSLLRQRNVENARRERLIARSCSKKE
                     IRKGHSQE"
     gene            complement(2930..>3594)
                     /locus_tag="PK898_08835"
     CDS             complement(2930..>3594)
                     /locus_tag="PK898_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU20176.1"
                     /translation="GVAGVVGAGRTELVSTIFGRDKSLGGKVYLDGKDITGFSTARVL
                     EAGINYVPEDRHYHGLFCISTIAANTTSALLPTPEMGNFILDTKNEYSITQRFIDLFR
                     IKVTDQKQTASSLSGGNQQKVVISRALSTEPKMVIMDEPTRGIDAVARGDVYNIIHQL
                     REKGVAVLLVSSDIEEIVQLSDRVVTVFQGRINGEFSKDEITVDNLTIASFGLTKDEA
                     MV"
     assembly_gap    3595..3694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3695..4200)
                     /locus_tag="PK898_08840"
     CDS             complement(<3695..4200)
                     /locus_tag="PK898_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU20177.1"
                     /translation="METGESKKLLSIRGITKAFGQNLVLKGVDMDLCGGEILALIGGN
                     GAGKSTLMKIIMGIYQPDAGCIFINGKEVQIINPSVALEECVYMVPQEPMLFPNMSVF
                     ENIIMGFHESNATLKARLQEIMADIGWQMDLTRKAITLSIAEQTLVEILRGLMRNAKI
                     LILDEPTSA"
     gene            4592..5524
                     /locus_tag="PK898_08845"
     CDS             4592..5524
                     /locus_tag="PK898_08845"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOU20178.1"
                     /translation="MELEEDLMSKIAWYYYIDGLTQQQIADLIGIPRLRVIRLLDKAR
                     QTGIIQFKIRKDNSNTELEHELARRFGLKDVFVVPSGSEKIGDRNEAIAQAAAMYISD
                     RLPDNAFINMGYGDTSSKILNHLANKQGQRINVVSLTGGVNYYLPNTQSNIFSAKLYL
                     TPAPFIMSTKEMVQAINNEPAVREIAEMVPLSTMSVVGIGAMNETATVIRNGIFTKKE
                     LLYLTMQGAVGDVLCHYFDINGNQVASEVEDRLITTSLETLAKQDNVIGVAAGPQKVN
                     AIIGALNGKFLDILITESEIAETILEKTQQLAVP"
     gene            5543..7108
                     /gene="lsrK"
                     /locus_tag="PK898_08850"
     CDS             5543..7108
                     /gene="lsrK"
                     /locus_tag="PK898_08850"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: protein motif:HMM:NF008187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HOU20179.1"
                     /translation="MTKKYLMAVDAGTGSVRAVLFDLDGQQIGCVQQEWTHKEDPRYP
                     GSMDFDWITNWQLTCHCIRNVILEARIDANEIAAVSTTCMREGIILYDKEGKEIWACA
                     NVDARSNDEVTQLKAMDPDLEKEIYLQSGQTFALGALPRLLWVKNNMPEIYKAIHSVG
                     MFNDWLIYKMTGKLAVEPSNGSTTGIFDLRKRTWAPEIAEKCGLRSDIFPPVIECGNV
                     LAEVNDRGAADTGLTAGTPVVVGGGDCQLGTIGVGAVSIGEAAVFGGSFWQYEFNTGD
                     GTTDPLCRVRVNCHAVPGVWQYEALAFNPGLVMRWYRDAFCQAEKQRGKIEGKDPYDL
                     MNSEAAKIPAGSYGMMCCFSDVMNFINWQHAAPTFTNFELDSQKYNRYTFYRAILENT
                     AMVTKGHKDLVGEATGSQPREIIFAGGASKSALWSQILSDAMGLQVKVPVVKEATALG
                     AAILAGYGVGLYKNIRETARQVVKIERTFSPNMENHKIYEQQYEVWKKVYAAQLDLCK
                     QGLTKNMWKAPGV"
     gene            7133..7495
                     /locus_tag="PK898_08855"
     CDS             7133..7495
                     /locus_tag="PK898_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU20180.1"
                     /translation="MYIVDEKDHEYRSGENGPKYLMKGPRMNFAIVQFMPDQDFTAHY
                     HNIMEENFYIMEGEIDIIVDNTVNHLTKGQFIHIEPGEVHYCINRSDKPVRMVSALAP
                     FVEKDKVEVTDFIYNKKN"
     gene            7877..8416
                     /locus_tag="PK898_08860"
     CDS             7877..8416
                     /locus_tag="PK898_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20181.1"
                     /translation="MKKIIISLILIVLFIPVSVFGQESTEKDEYVPNFPIPEYFKIDT
                     CLSQMKEEEGNETADLTTSLLMASLGAKGGTTAEEWCQFEIKEETHSPELVKEINSFY
                     EKEYKDQKFKLAGEPELLPMNIMGEELGKAYQSVLSNGKENIWIVVWHVDDNQELLDA
                     ESGNKPNGSAIGYIFFSKM"
     gene            8690..9229
                     /locus_tag="PK898_08865"
     CDS             8690..9229
                     /locus_tag="PK898_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOU20182.1"
                     /translation="MNNNILIAYFSRADKNYFGGKIVLLPIGNTEVVMKKISEQIGGT
                     LFKINPLKKYSEDYQICTEEAKKELNENARPPLSDYLDKVEIYNPIILGYPIYWGTMP
                     MPVWTFLEHYDFSGKTIFPVCTHEGSGLGRSEEDIRKICPAANVRKGFAIRGSDVNRA
                     QNEIDRWLKEQGLFHSQTD"
BASE COUNT         2915 a         1951 c         1996 g         2473 t
ORIGIN      
        1 caagccaaag cgccctgtag accacagtcc cataaccccc accgtgcgat ggtaccggct
       61 tgaaggtgag cggtcattcc ggatggcggg cagaatccgg tgatggtgat atcatccttt
      121 gtcagtcctt tgttttccgc agcggtgcac tgccccggaa gagcggttga gtcattgcag
      181 ataatcaggt caatatcggg atgagcatcg agaatcgctt ctccgattgc taccgctttt
      241 tgcggatctt gttcggaata atacggatcc gcaacagcta cccagttcgg atatttttct
      301 ttaatataag cttcaccggc aacgtaccat gaattttgat cggcaacggc agcctgagaa
      361 taatgccata catatttgac cggaccgttc acatcaacac cgcgttcttt taaggattca
      421 acgcccattt caacaagcat cgggccgagt tgagaagccg taccttgaga aaccattaag
      481 ctacgcgcat cggaagaaac atcggaatcc caggtaacga ccacaatacc ttgctcctga
      541 gcttctttca atttttcatc cagcgctttc gcatcgacgg aagaaatcgc aatagcatcg
      601 acgccggcat caattgcctg ttggatgact tccaattgat cggttacgga agctgtggca
      661 ctgcccatat atttcaccgt aatgccccat ttttcggcat atttctgggc acccgcatta
      721 gctgcctcaa aaaatgagtt tcccgtaact ttcgggatga acgcaacaac tttgcctttt
      781 atggaatttt cggcaaagac tgttgatacc ccgacaagtg aaacaataag cactgtcagg
      841 acaataaaac gaaatactga ttttttcata ctttccctcc aaaaattttt agaataaact
      901 gcgtcagtct aaatgatgta caatacggtt gatctgacgc agactaacca ccaagataag
      961 catgatacct accggaagat caagattttg gctgctgatg ttaaagcata aggtaagccc
     1021 aaatcgaagt aaacaaatta ccaaagccgc caaagcggtc cctataacac tacctttgcc
     1081 gcctgatgta agcgttccgc ccaacaccac agccgtaata atcgagagag taaaggtgga
     1141 acccaaatta tatttagcgg aatccacttg agccgtcaaa agaatacccg caatagcggc
     1201 accgataccg gtcagtacat atgtactgaa aataatcagt cgactgttaa ttccggaata
     1261 ttcggcggct ttcggattgt cgcctaccaa aaatatcctt cttccatatt tagattgatg
     1321 taacagtaca tagcaaagga taataagaat cagataaata acaatctgaa tcggaatcaa
     1381 atcaaaaagc ttcgcttttc ctaagaaacg gaaagagata taatcaccgt tcgcattctt
     1441 ccccggaaat ccgctgatac cctgatatgc gggaatcttc gaaaaattcg agatcacttg
     1501 tgccaacccg gaataaagga aactgccgcc taacgtgacc accatcggtt gaacatgaca
     1561 gtaagcgata aaaaatcccg aaagagcccc gcaaaatgcc gctaaaagga cagcgattgc
     1621 taccgccatc cagatattca aacccgcatc attccataga acaccgatcg ttatggaagt
     1681 taagcctaca atggaacttg cctgaatatc gataccgccc gtgatcataa caaaagttac
     1741 gaataatgaa ataatacaaa tgctgatgta gttattgata tttgaaaata ttgaggcaat
     1801 tcgaaagaat ttcggattaa tactgccgaa tattacaaac tctaaaatca ggattaccag
     1861 aagagtaaat tcccagtttt tgaagatatt ggtaagctta ttcttcattc ttgactgtgc
     1921 cctttccgga tctctttttt ggaacaggat cttgcaatca gacgttcacg gcgtgcgttt
     1981 tccacattcc gttgccggag caaagaatca agcacaacga tcgtaattaa caggatgcct
     2041 gtaatcgtgt tatcgtattc ggatgaaaat cctaaaaata ccagcaggcg gctgattgac
     2101 gacatgatga cagcaccgat agccgcacca atcacgtttc ccaaaccgcc ggaaagggaa
     2161 attcctccca aaacacaggc ggctattgcc gacatttcat aaccgctgcc tgcgataatg
     2221 gtaacttgtc cgtattttga ggaaaacgcg atacccgaaa ttgccgcgaa aataccgcaa
     2281 aggatatacg caatcagttt tgtccgttta gcggggattc cgaccaatat tgcaccgtca
     2341 taattatcac cgactgccag aaaatatttt ccttgcctcg ttttagtttc aacgacatta
     2401 attattaaga cgattacggt aacaatcaaa agataaatac cgattccggt attcccgaca
     2461 gtcgagttgc tgaaacgtgt gaaagcgccg gaaaaattct ctacggttcg tccgccactg
     2521 taaatataga ccaatccgcg aacgatgcca taggtaccga gtgtaaagat cagagaagga
     2581 actttcaaaa tcgcaacacc gacaccgttt acaaaaccga cggctaaccc gagcaaaatg
     2641 gcatagacaa ccatcattac ccaactgcca ttttgctggg caatggaacc ggcaatcgct
     2701 gccgataaac ccattgtgga gccgatcgaa acatcgattt caccggtcat aataacaaac
     2761 gaaataccga ctgccaaaag gataaaaacg atactgctgt tgaaacaggt aataatgttg
     2821 ccgtaagaca gaaaagtcgg attgatcgct cccgtaataa ggaacaaagc aatcaggaat
     2881 aaaagactgg aaaaccagcg tgattccgtg agaactttga taatcttatt cataccatcg
     2941 cctcgtcctt ggttaatcca aaagatgcta tggtcagatt gtctaccgta atttcatctt
     3001 tggaaaactc gccgttaatt cgtccctgaa atactgttac gacccgatca gaaagctgaa
     3061 cgatctcttc aatgtccgag gaaaccaata acaccgcaac ccctttctct cggagttgat
     3121 gaataatgtt ataaacatca cctcgggcga cagcatcgat accgcgggtc ggttcgtcca
     3181 taatgaccat tttcggctcc gtcgagagtg cacggctgat gacaaccttc tgttgatttc
     3241 ctcccgagag actgctcgcg gtttgtttct ggtcggtaac tttaatccgg aaaagatcaa
     3301 taaacctttg cgttatggaa tattcatttt ttgtgtccaa gataaaattt cccatttcag
     3361 gagttggaag aagggcggaa gttgtattcg cggcgattgt gctgatgcaa aagagtccgt
     3421 gataatgacg atcttccggg acataattga ttccggcttc cagaactctt gcggttgaaa
     3481 agccggttat gtccttccca tcaagataga cttttcctcc aagagattta tcacgcccaa
     3541 aaatggtact gacaagttcc gttcgtccgg caccgacgac accggcgaca cccannnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcggaa gtcggctcgt caaggatcag
     3721 gatttttgca ttccgcatca gcccacgtag aatttcaacc aacgtctgtt ccgcaatgga
     3781 aagcgtaatc gcctttcggg ttaaatccat ctgccatccg atatccgcca taatctcttg
     3841 aaggcgagcc ttcaaggttg cattggattc gtgaaagccc ataatgatat tttcaaaaac
     3901 gctcatgttg ggaaacagca tcggctcttg gggaaccata taaacacatt cctctaaagc
     3961 gacggaaggg ttaataatct gaacttcttt tccattaatg aaaatacatc cggcatccgg
     4021 ttgataaatg cccataataa tcttcattaa ggtgctcttt ccggcgccgt tgcctccgat
     4081 caatgccaat atttccccgc cgcaaaggtc catatcaaca ccttttagta caaggttctg
     4141 tccgaaagct ttggttattc ctctgatcga cagtagtttt ttggattcac ccgtttccat
     4201 aaatacggtg ctcctatttt caaagaatca ttctcccaga taaatacttt tgtgtagcaa
     4261 ccatacaata agtatgtaac taagatacca gtattaacct tgtttgtcaa tagttaattc
     4321 gacgctattt ttcatgacga ttgataaaag tcaaaattta tctcgtattt atgagccccg
     4381 tctttgattt ttccctaagt tttacactaa ccattgacaa gcttttttct cttcattata
     4441 ataacccaaa gtgaacattt atttacaata cgatacaaat gtttatttat tgaaagacga
     4501 tcaaacactg taatccgtat tggcgttgag accgagtttt ttgtaaacag gaaaaagttt
     4561 tgtacataat tgaaatgaaa ggcaatttaa tatggaacta gaagaagacc tgatgtcgaa
     4621 aattgcttgg tattactata tcgatggtct cacccagcaa cagatcgccg atctgatcgg
     4681 gattccaaga ttaagagtca ttcgtctgtt agataaagct cgtcaaacgg gaattatcca
     4741 gttcaaaatt cgtaaagata attcaaatac cgaattggaa catgaactcg ccagacgatt
     4801 cggattgaaa gatgtcttcg ttgttccatc gggttcggaa aaaataggtg acagaaatga
     4861 agcaatcgca caggcagctg ccatgtatat cagtgatcgc ctgccggata acgcttttat
     4921 aaatatgggg tacggagaca cttcaagtaa gatccttaac catcttgcca acaaacaagg
     4981 acaaagaatc aacgtcgttt cgttgacagg aggggtaaat tattatttgc ccaataccca
     5041 atccaatatt ttcagtgcaa aactctatct gacgcccgct ccgtttatta tgtcaacaaa
     5101 agaaatggtt caagccataa ataatgaacc tgctgtccgt gaaattgcgg aaatggtccc
     5161 gctctcgaca atgtcggtgg tcggtattgg cgcgatgaat gaaaccgcca ccgtaatacg
     5221 gaacggtatt tttacaaaaa aagaattact ttatctaacg atgcaagggg cggtcggtga
     5281 tgttttatgt cattacttcg atatcaacgg gaaccaagtt gcttcagagg tcgaagatcg
     5341 tttgatcaca acttctttgg aaacattggc aaaacaggat aatgttatcg gcgtggctgc
     5401 cggtccgcaa aaagtaaatg ccatcatcgg tgctttaaac gggaaattcc tcgatattct
     5461 gatcaccgaa agcgagatcg ccgagacaat tcttgaaaaa acgcaacaat tggccgttcc
     5521 ataacaataa aggaggcaaa aggtgacaaa aaaatatctt atggcggtag atgcgggtac
     5581 cggaagcgta cgggcggttt tgtttgacct tgacggtcaa cagatcggct gtgtgcagca
     5641 ggaatggact cataaagagg atccccgata tcccggaagt atggattttg attggattac
     5701 caactggcaa ttgacttgtc actgcatccg taacgttatt ttggaagccc gaatcgatgc
     5761 aaacgagatc gcagcggttt caacaacctg catgcgcgaa ggaattattc tctatgataa
     5821 ggaaggcaaa gaaatttggg cttgcgcgaa cgttgatgcc cgcagcaatg atgaagtgac
     5881 acaattaaaa gcgatggatc ccgacctcga aaaagagatt tacttacaat ccgggcaaac
     5941 cttcgcttta ggcgctttgc cgcgccttct atgggtgaaa aataatatgc ccgaaatcta
     6001 taaagcaatc cattccgtcg gcatgttcaa cgactggctg atttacaaaa tgacggggaa
     6061 attagcggta gaaccttcca acggttcgac aaccggcatt tttgacctgc ggaaaagaac
     6121 ttgggcaccg gaaatcgccg aaaaatgcgg cttacgcagc gatatttttc ctccggtaat
     6181 cgaatgcggg aatgtcttag ctgaagtaaa tgatcggggg gcagcggata caggattgac
     6241 agcgggaact ccggtcgttg tcggtggggg cgattgccag ctgggtacaa tcggcgtcgg
     6301 tgcggtttcg atcggcgaag cggctgtttt cggcggcagt ttttggcagt atgagttcaa
     6361 tacaggggac gggacgacag atccgctttg tcgcgtgcgg gttaattgtc acgcggtccc
     6421 cggagtgtgg caatatgaag cattggcatt taatccgggg cttgtgatgc gctggtatcg
     6481 tgatgctttt tgtcaggcag agaaacaacg gggaaaaata gaagggaaag atccgtacga
     6541 tctgatgaat tcggaagcag caaagattcc ggccggaagt tacgggatga tgtgttgttt
     6601 ttcggatgta atgaatttta taaattggca acatgctgct ccgactttca cgaattttga
     6661 gttggattca caaaaatata atcgttacac cttttatcga gcgattcttg aaaacaccgc
     6721 catggtaacc aaagggcaca aagatctggt cggcgaagcg accggcagtc aacctcgcga
     6781 aatcatcttt gcaggcggag cttcgaaaag cgccctctgg agtcagatcc tttcggatgc
     6841 gatgggctta caggttaaag ttccggtggt caaagaagca accgcattgg gcgcggctat
     6901 tttggccggc tatggcgtcg gtttatataa aaacattcga gaaaccgccc ggcaagttgt
     6961 aaaaatcgaa agaacatttt caccgaacat ggaaaaccat aaaatttatg agcagcaata
     7021 tgaagtttgg aaaaaagttt atgccgcaca acttgattta tgtaaacaag gattaaccaa
     7081 aaatatgtgg aaagcacccg gcgtttaaat gctcggaagg agaaataaga atatgtatat
     7141 agttgatgag aaagatcatg aataccgctc cggagaaaac ggaccgaaat atctgatgaa
     7201 gggtccgcga atgaattttg ctattgtcca atttatgccc gatcaggatt ttacggctca
     7261 ttatcataac atcatggaag aaaattttta cattatggaa ggtgaaatcg atattatcgt
     7321 tgacaatacc gtcaatcatc tgaccaaagg tcaatttatt catattgaac cgggagaagt
     7381 tcattattgt attaatcgtt cagataaacc ggtaaggatg gtttctgctt tggcaccttt
     7441 cgtggaaaag gacaaagttg aagttactga ttttatttat aataaaaaaa attaatccaa
     7501 atcagtctgc aagatcgtca taactgattt gtccaatttg aagaaattta agaggacgaa
     7561 agcggaaata catcggtttt tgccctcttt tttcatataa ttttaaagtc attgatttaa
     7621 gtacaatcga tccttattgc tccgattatc ccttttctaa ttcgacattg acctccgccg
     7681 ggggaatccg tataatttaa ttacaataat ttaattaaca aaatcggctg cgggaatttt
     7741 ttatcggcag tttaaagatt tattcattca taaaaagttt cgattctcga ataaaaaagt
     7801 tcattagccg agaattattg tctaattatc gtaaaagagt tttgttttca tgaccagtta
     7861 aaaagggagg aaaaccatga aaaaaataat aatcagcctg attttgatcg ttctattcat
     7921 cccggtgtct gtattcggac aggaatcaac cgaaaaagat gaatatgttc ccaatttccc
     7981 gatcccggag tatttcaaaa tagatacctg cttgtctcaa atgaaagaag aagaagggaa
     8041 tgagacagct gatttaacaa cttcactatt aatggcctct ctgggcgcca aaggtgggac
     8101 taccgcagaa gaatggtgtc agtttgaaat taaagaagag actcattcac cggaattggt
     8161 taaggaaata aattcctttt atgaaaaaga atataaagat cagaaattta aattagccgg
     8221 tgagccggag cttctgccta tgaatattat gggggaagag ttgggaaagg cttatcaatc
     8281 cgttttaagt aacggcaaag aaaatatctg gattgttgtt tggcatgttg atgacaacca
     8341 agaattatta gatgccgaaa gtgggaataa accaaacggt tccgcaatcg gatatatctt
     8401 tttctcaaaa atgtaaattg tctttttcgg ttgttgaaag tacacagatg atccgggcaa
     8461 atctctgata ttgcttatta gaaagcaaaa cagagaacga gattcggttt tggtttttat
     8521 tggatcagaa attcacaacg gtaaaactct caacacgtct aaaaaaattg gctgaaattt
     8581 cagccaattt ttttagacga atatggattg ttggctcttc acgaaaatcc tcttgactta
     8641 gagtaagctc caagttatac aatgatgatc atcaataaag gaaaagaata tgaacaataa
     8701 tattttaatc gcatattttt ctcgagcaga taagaattat ttcgggggga aaatcgtttt
     8761 gttgcccatc gggaataccg aagtggttat gaaaaagata tcggagcaaa tcgggggaac
     8821 actgttcaaa attaatccgc ttaaaaaata ttcagaagat tatcaaatct gtacggaaga
     8881 ggcaaaaaaa gaactgaatg agaatgccag accaccatta agcgattatt tagataaggt
     8941 agagatttac aacccgatta ttctcggtta tcccatttat tggggaacaa tgccgatgcc
     9001 ggtttggact ttcctcgagc attatgactt ctcgggaaaa acaattttcc cggtttgtac
     9061 acatgaaggt agcggtttgg gacgcagcga agaggatatt cgaaaaattt gtccggcggc
     9121 aaatgttcgt aaaggattcg ccattcgcgg cagtgatgta aatcgtgccc aaaatgagat
     9181 tgatcgatgg ctgaaagaac aaggtttatt tcacagccaa acagactgac cataaaagtg
     9241 aacagaaaaa gatctttttt ggattttcct ttacatccaa aaattcttta aaaataccct
     9301 tgacttagag taagctccaa gtattacact gaaaaacaga tcaagagccg atcaagagca
     9361 aataaagcga ttcaatgatg tcgacccgga aaggatagaa actcggatga aaaaaacaat
     9421 ttctcgcagg gaagc
//
LOCUS       DAOSMM010000072         9211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_7458_length_9211_cov_16.855614, whole genome shotgun sequence.
ACCESSION   DAOSMM010000072 DAOSMM010000000
VERSION     DAOSMM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7458_length_9211_cov_16.855614"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..817)
                     /locus_tag="PK898_08870"
     CDS             complement(95..817)
                     /locus_tag="PK898_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20183.1"
                     /translation="MLKINNLKVSYGGIKALNGISLEIPDGKIVTLIGANGAGKSTTF
                     KAIVGLVKPVSGEIWFKDQNITGLPTNKIIGSGISTVPEGRKVFSDMSVLENLRIGAY
                     LRKDEEGIRRDIDMVFDLFPRLKERHWQQAGTLSGGEQQMLAVGRGLMGHPQLMLMDE
                     PSLGLAPLIVQEIFSIIKKINDEGVTILLIEQNANMALRTADYAYVMETGHITMQGTG
                     RELLAEESIRSAYLGKRRKAKV"
     gene            complement(801..1649)
                     /locus_tag="PK898_08875"
     CDS             complement(801..1649)
                     /locus_tag="PK898_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20184.1"
                     /translation="MTESVLRIDHCTMQFGGVVAVDDLCMEINKDEIVALIGPNGAGK
                     TTAFNFVTGVYEPTNGRVYFNDKLLVANHPQGKMKKLYMGEFEKICPDVVRKTPDQIT
                     RLGIARTFQNIRLFKNLTVLENVLIAMHLHMKSGLIAAATHLNYKEENQMRKEALELL
                     EMQNLIHVKDEIASSLPYGQQRHLEIARALATKPTLLLLDEPAAGMNPQETDELAEFI
                     RKTKEDFNLTVFLIEHHMTLVMDISDRIYVLDFGKMIAHGSPDAVQNDERVIHAYLGV
                     ADAEDQ"
     gene            complement(1642..2736)
                     /locus_tag="PK898_08880"
     CDS             complement(1642..2736)
                     /locus_tag="PK898_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU20185.1"
                     /translation="MTLKKSTKTILSITVLVLIALLLFHLEQDKRANSYILSILERSI
                     IYAVVAVSMNLLNGFTGLFSLGQAGFMALGAYTVGILTIPVENRPSVYYTSGINETIA
                     NWHLPFLPALLMGGLVAALAAALVGALILKLKSDYLAIATLGFSEIVRAVIASPQLDM
                     ITNGSYGLKKIPRFTTVYIPFALAAACILLMILLINSSYGRAFKAIREDEVAAEAMGI
                     NLFKHKELSFVISSFFTGIGGGMLACYMGSIDSGTFRITLTYDMLLIVVLGGLGSVTG
                     SILGSFLVTAGREWLRFFDEPHAIFGWQIPLFRSGFRMVVFSVLLMVVVLFYRKGIMG
                     DKEFSWESFFGFFKRLIPKKKKLAGGNTHD"
     gene            complement(2736..3617)
                     /locus_tag="PK898_08885"
     CDS             complement(2736..3617)
                     /locus_tag="PK898_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU20186.1"
                     /translation="MSLTTILQHSLTGISLGGAYALIAVGYTMVYGILRLINFAHGEI
                     FMMAGYFMIFSMASFPWYISIPIVLAGTALLGVSIEKVAYKPLRSAPRMSVMISAIGV
                     SYLLQNLATYLFTALPRGYPEIPGLKKVFQIGDLSSSVVTFVTPVVTILLAIVLVQMI
                     NHTKIGMAMRAVSKDLDTAQLMGIQINSVISFTFLIGSLLAAIGSILYFTDRMSVNPF
                     SGSLPGLKCFVAAVLGGIGSIPGAIVGGFILGIGETFMVAAGFSTFSDAFTFIMLIFM
                     LLVKPTGLFGEKEPEKV"
     gene            complement(3824..4987)
                     /locus_tag="PK898_08890"
     CDS             complement(3824..4987)
                     /locus_tag="PK898_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20187.1"
                     /translation="MKKFFSLVILLIVVISAVGFAPVTQDENVVTFGVFEPLTGENGG
                     GGYQEVLGIRYANKVYPTVEAGGKTYTIKLYEVDNKSDKTEAVTAAQNIVSKNVNAII
                     GSYGSGVSIAAGAIFEEAQIPAMGASCTNPQVTYGNDFYFRVAFLDPFQGTVMANYAF
                     QNGAKTAAVITQLGDDYSSGLGKFFVDAFKKLAGDAAIVAEEQFQTNQTDFKAILGNI
                     KAKNPDVIFAPSSIVTAPLIIKQAREMGITAKIMAGDTWENATIIENAGKDAEGVVFS
                     TFFDEADPATEEAKKFVEGFKPFLVENNQPDVIPGVSALGYDAYLALYKAIEAADSVD
                     PIAIRDALVTLEFDGVTGHIKFDENGDAIKNMAVIKVIQDGAFKFLQTVTVSE"
     gene            complement(5196..6908)
                     /locus_tag="PK898_08895"
     CDS             complement(5196..6908)
                     /locus_tag="PK898_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU20188.1"
                     /translation="MKTRWGNNLNPDAILSEYPRPQMVRDHYLILNGFWNYAITESSE
                     QPAQFDGDILVPFSPESELSGVNRILTKDQYLWYRREIVLPDDFLLDHLLLHFRAVDQ
                     KATVYLNGIEVGSHLGGFTPFSLDITMAWQKNDRNILTVKVQDTTDFSWNTRGKQRWK
                     PGGIWYSPQSGIWQTVWLESVPSDYIQSIQITPDFDAACVRFFICSERNLPCLLEISG
                     SEFRFPSNEPVTVPMPNFIPWTPDQPYLYSFTAQLADDKVNGYFGMRKFSVGSDSDGK
                     KRFFLNNQPIFLSGLLDQGYYSDGLLTAPSDEAMIYDIQTAKSMGFNLLRKHVKIEPL
                     RWYYHCDRLGMIVWQDMPNGGKAYKDWLISAPVVVNFRINDRHYKWLGRKEKASREQY
                     LVELEEMIRHLYNVVSIGMWVPFNEGWGQFDAVEVTKQIQKMDPSRTIDSASGWFDQF
                     TGDFLSRHVYFKKYRYKKDKLNRAVILSEFGGYSYRVEGHVYSENNFGYKQFKSEEEF
                     QKAFISLYENEVIPFKVKGLTAAVYTQLSDVEDELNGLLSYDREVIKISPEIVQEINQ
                     RLLS"
     gene            complement(6886..8169)
                     /locus_tag="PK898_08900"
     CDS             complement(6886..8169)
                     /locus_tag="PK898_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU20189.1"
                     /translation="MNLTRKQWLSLIFIGLTGQLAWMIENMYLNLFMYQTASTDPHYI
                     ALMVSASAVVATLTTLLMGNLSDRFGKRKVFISAGYILWGISIILFSFVNRENIQALY
                     PFANASVVAAYGIVVLDCIMTFFGSTANDAAFNAYVTDITVPTNRGKVESVLATFPLI
                     AMLIVFGCFDPIVQQGNWNLFFRIAGGIVLAVGFLSLFFIKEVPVEKKKGNFFAELFY
                     GFTPASFHENKNLYIIMIAFCIFGAAFQVFFPYLIIYIQQFLFIENYALILGTILIIA
                     SLISILMGPVIDKYGKMEVIYPAIITMLIGLVGLFFSRRNIPVTFWGIIMMSGYMMIS
                     ATLSALIRDFTPPGKVGVFQGVRMIFTVMLPMVIGPYIGAWLIKSNGGIYEELGQTKN
                     IPTPDIFLVSAFVLCFIVIPLYFLGKNQHENKMGE"
     gene            8348..8767
                     /locus_tag="PK898_08905"
     CDS             8348..8767
                     /locus_tag="PK898_08905"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU20190.1"
                     /translation="MPTRKSQAIWEGSLKEGKGRMMLGSGAFEGKYSFGSRFEEEPGT
                     NPEELIAAAHAGCFTMFLSSLLTDAGFTPRKITTTAKVNLAAGPEISKIELETEADVP
                     GISDKVFQEQALIAKKNCPISKALSAVPEITLSAKLV"
     gene            complement(8830..9138)
                     /gene="rpsJ"
                     /locus_tag="PK898_08910"
     CDS             complement(8830..9138)
                     /gene="rpsJ"
                     /locus_tag="PK898_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOU20191.1"
                     /translation="MAKQKIRIRLKAFDHRILDQSAKRIVDTAERSGAHVVGPVPLPT
                     RIERFTVRRSTFIDKDSQEHFEIRTHNRMIDVLDPDSKTIDMLMRLNLPAGVDIEIKM
                     "
BASE COUNT         2811 a         2160 c         1760 g         2480 t
ORIGIN      
        1 ggtctgattg atcgtctctc ggatttttac tgaaataaaa agatcgggat cgttccttgt
       61 aaaccgatcc cgatcctcac gataaaaaat caaattaaac ttttgctttt cttcgttttc
      121 ccaaataagc cgatcgaata ctttcttcgg caagcaattc ccttcctgta ccctgcatag
      181 tgatgtggcc ggtctccatt acataagcgt aatcggccgt tctcaaagcc atattggcat
      241 tctgctcaat caataaaatc gtaacacctt catcattgat tttcttaatg attgagaaaa
      301 tctcctgaac aattaaaggc gccagaccga gagagggttc atccatcaac atcagctgag
      361 gatgacccat caatccgcgc ccgactgcca acatttgttg ttcaccaccc gaaagggttc
      421 ccgcttgctg ccaatggcgt tctttcaaac gagggaagag atcgaatacc atgtcgatat
      481 ctctgcgaat tccttcttca tcttttctca aatacgcacc gatccgcaaa ttctcaagga
      541 cactcatatc ggaaaatact tttcgccctt ccggtactgt tgaaataccg ctcccgataa
      601 ttttatttgt cggcagaccg gttatatttt gatctttaaa ccagatctcc cctgaaaccg
      661 gcttgaccaa tccgacaatc gctttgaaag tagtgctttt gccggcacca tttgcaccaa
      721 tcaacgtaac aatctttccg tctggaattt cgagagaaat accgtttaaa gctttaatgc
      781 cgccgtagga aactttcaaa ttattgatct tcagcatctg ccacccccaa ataagcatga
      841 atgactcttt catcattctg aacagcatcg ggagaaccgt gggcaatcat tttcccgaaa
      901 tccagcacat aaatccgatc ggaaatgtcc ataacaaggg tcatatgatg ctcaatcaag
      961 aaaaccgtca aattgaagtc ctctttggtt tttcgaataa attcggcaag ttcatccgtc
     1021 tcctgaggat tcattccggc agccggttca tccaataaaa gcagagtcgg tttggttgcc
     1081 aacgctctgg cgatctccag atgacgctgc tgtccgtagg ggagagagct tgctatttca
     1141 tcttttacat gaatcagatt ctgcatttcc agcaattcca acgcttcttt gcgcatctgg
     1201 ttttcttctt tataattcaa atgggttgcg gcagcgatta atcccgattt catatgcaaa
     1261 tgcatggcaa tcaacacgtt ttcaagcacg gtcaaattct taaacaatcg gatattctga
     1321 aatgtccggg caatcccaag tcgggtaatt tgatcaggtg tctttcgaac cacatccggg
     1381 caaatttttt cgaactcgcc catataaagt ttcttcattt ttccttgcgg atgattggca
     1441 accaataatt tgtcattaaa ataaactctg ccgttcgtcg gttcgtaaac acccgtaaca
     1501 aaattaaaag cggttgtttt cccggctccg ttcgggccga tcaaagccac aatttcatcc
     1561 ttattaattt ccatgcataa atcatcgaca gccactacac cgccaaactg catcgtgcaa
     1621 tgatcaattc tcaagacgga ttcagtcatg cgtatttcct cccgcaagtt tcttcttttt
     1681 cgggattaat cgtttgaaaa atccaaagaa actctcccaa ctgaattctt tgtcacccat
     1741 gattcctttt cgataaaaca acacaaccac catcagcaga acggaaaaga caaccatgcg
     1801 gaatccggaa cggaataaag gaatctgcca tccaaaaata gcatgcggct cgtcgaagaa
     1861 gcgcaaccat tcccgaccgg ctgtaactaa gaatgatccc aatatggatc cggtaacgct
     1921 gcccaacccg cctaaaacaa caatcagcaa catatcataa gtcaacgtga ttctgaaagt
     1981 gcccgaatcg atcgatccca tataacaggc taacattcct ccgccgattc cggtgaagaa
     2041 tgagctgatc acaaaagaaa gctctttatg cttgaataaa ttaatcccca tcgcttcggc
     2101 ggcgacttca tcttctcgaa tggctttaaa tgcccttccg taggaagaat taataagcaa
     2161 aatcatcagt aaaatacagg cagccgctag agcaaacggt atatataccg ttgtaaaacg
     2221 ggggatcttt tttaatccat acgacccgtt ggtaatcata tccagttgcg gactggcaat
     2281 tacagcccga acgatttccg aaaaaccgag agtggcaata gccaaataat cgctctttag
     2341 tttcaagatt aaggcaccga ccagcgcagc cgccaaagct gccaccaacc ctcccatcaa
     2401 taaagccggc agaaaaggca gatgccaatt ggcaatcgtt tcatttattc cgctggtata
     2461 gtataccgac ggccggtttt cgaccggaat ggtcaatata ccgacggtat aagctccgag
     2521 cgccataaat ccggcttgtc cgagagaaaa taatccggtg aaaccgttca gcaggttcat
     2581 cgatacagcc actaccgcat agatgatgct gcgttccaat atggaaagaa tataactgtt
     2641 ggctctttta tcctgttcca gatgaaacaa aagcagggca atcaggacca gaaccgtgat
     2701 cgataaaata gttttggttg attttttcag tgtcatcaga ctttctccgg ttctttttca
     2761 ccgaacaacc ccgtcggttt taccaaaagc atgaaaatga gcatgataaa tgtgaaagca
     2821 tcactgaaag tggaaaagcc cgcggcaacc ataaaagttt caccgatccc caatataaag
     2881 ccgcccacaa tcgcacccgg aatactgccg atgccgccca gaactgccgc gacgaaacac
     2941 tttagtcccg gaagggaacc gctgaaagga tttaccgaca tcctgtcggt aaaataaagg
     3001 atggaaccga ttgccgccaa cagcgatccg atcaaaaatg tgaagctgat tacggaatta
     3061 atttgaattc ccatcaactg cgcagtatcc agatctttcg atacggctct catagccata
     3121 ccgatttttg tgtgattgat catctgaacc agaacaatag ccagcaggat cgtgacaacc
     3181 ggggttacga aggttacgac cgaagaagaa agatctccga tttggaaaac tttcttcagt
     3241 ccgggtattt ccggatatcc tcgcggcaaa gccgtaaata aataggttgc gaggttttgt
     3301 aaaagatagg aaacgcctat cgcagaaatc ataacggaca tccgcggtgc ggaccgaagc
     3361 ggtttataag ctactttttc aatggaaact cccaataaag ccgtcccggc tagaacaatc
     3421 gggatggaaa tataccaagg aaatgaagcc atagagaaaa tcatgaaata gcctgccatc
     3481 ataaaaattt caccgtgagc aaaattaatc aggcgcagaa ttccgtaaac catggtatag
     3541 cccaccgcaa tcagtgcata tgcaccgcct aatgaaattc ctgtcaggct atgttgcaat
     3601 attgtcgtca aactcataga aggttgcccc tcctttattt ccgtataatc tcacagcaga
     3661 aaacgggctg aaatatttga tcaaaaaatc acaaatctaa ttcagaccga aaagatgaaa
     3721 ggtgcctaag gcacctttca tccgaagcaa caaaacagaa ctaccttcgt tcaaggaaaa
     3781 taacgtgaag aaataacttc aacattcttt tcatgctgtc catttattca gaaacagtca
     3841 cagtctgtaa gaatttaaaa gctccatctt ggatcacttt aataaccgcc atatttttaa
     3901 tggcatcccc attttcgtcg aatttaatgt gaccggttac accgtcaaat tcaagagtga
     3961 caagcgcatc tcgaatcgct attggatcaa cagaatcggc agcttcaatg gctttatata
     4021 aagccagata agcgtcataa cctaaggcag aaacacccgg gataacatcg ggttggttgt
     4081 tttctacgag aaatggttta aacccttcca caaatttctt tgcttcttcg gtagccggat
     4141 cagcttcatc aaaaaatgtt gagaaaacga cgccttccgc atctttaccg gcattttcaa
     4201 taattgtcgc attttcccag gtatctcccg ccataatttt tgccgtaatg cccatttcac
     4261 gcgcctgttt tatgatcaac ggggcggtca caatggaaga aggagcaaaa ataacatccg
     4321 gattcttcgc tttaatgttc ccgagaatag ctttgaaatc cgtctgattc gtctggaact
     4381 gttcctcggc gacgatagcg gcatcaccgg ctaatttttt gaacgcgtca acaaagaact
     4441 ttcccaatcc ggacgaataa tcatcgccga gttgagtaat cactgccgca gtttttgcgc
     4501 cattctggaa tgcgtaattg gccattactg tcccttggaa cggatcaaga aaagcaactc
     4561 ggaaatagaa atcattccca taagtcacct gcgggttggt acaagaggct cccatcgccg
     4621 gaatctgagc ttcctcgaaa atcgcgcctg cggcaataga tacgcccgaa ccataagaac
     4681 cgataatcgc attcacattt ttgctgacga tattctgtgc ggcggttacg gcttcggttt
     4741 tatcactctt attatccacc tcgtagagct tgatcgtata agtttttccg ccggcttcaa
     4801 cggtcggata aaccttattg gcatagcgaa ttcccaaaac ttcctggtat ccgcccccgc
     4861 cattttcacc ggtcagaggt tcaaatactc caaatgtaac cacattttca tcctgtgtta
     4921 caggagcaaa accgactgcc gaaattacga caattaacag aattaccaac gaaaaaaatt
     4981 ttttcatagc tcctccgata gattgattcc ggaatactcc ggttttccaa acgctttcag
     5041 acttataaat gtatttttgc attatagcac gcaaagaaat tgttagcaat aaaatttgat
     5101 aatatcatca ttgcgaaatc aaagttgatg gattaaaaca atcattcttt ctaattcgac
     5161 ttattaaatt tatattctct atggaaataa aaaatttagg ataataatct ttgattgatt
     5221 tcctgaacaa tttcggggga aatcttgatc acctcacgat cataagataa taatccgttc
     5281 aattcatctt cgacatcgga aagttgtgta taaaccgcgg cagtcaatcc cttcacttta
     5341 aaagggataa cctcgttttc atagagcgaa ataaaagctt tttgaaattc ttcttcgctt
     5401 ttaaactgtt tgtagccgaa attattttca ctgtaaacgt gcccttccac acgataactg
     5461 tatccgccaa attccgacaa aataaccgct ctgttcaatt tatctttttt atatcgatat
     5521 ttcttaaagt aaacatgtct gcttaaaaaa tctccggtaa attgatcaaa ccagccgctg
     5581 gccgaatcga ttgttcgcga aggatccatt ttttgtatct gctttgtcac ttcaacagca
     5641 tcaaattgtc cccacccttc gttgaaagga acccacattc cgattgaaac gacattgtac
     5701 agatgccgaa tcatttcctc cagttcgaca agatattgtt cccgcgacgc tttttctttt
     5761 cttcccaacc atttataatg acggtcattg atacgaaaat tcaccactac cggagcggaa
     5821 attaaccaat ctttataagc tttccctccg ttaggcatat cctgccaaac gatcataccg
     5881 agacgatcac aatggtaata ccaacgcaaa ggctcaatct ttacatgctt tctcaataga
     5941 ttgaatccca ttgattttgc ggtttggata tcataaatca ttgcttcatc cgacggagct
     6001 gttaacaatc catcactata atatccctga tccaataaac cggataaaaa tatcggttga
     6061 ttattcaaga aaaatcgttt cttcccatcg gaatctgaac cgactgaaaa ttttcgcatt
     6121 ccgaaataac cgttgacttt atcatcggcc aattgagccg taaacgaata taagtaaggt
     6181 tgatcgggag tccagggaat aaaattcggc atcggtacgg tcaccggttc attcgatggg
     6241 aatcggaatt cgctcccgct gatttcaagg agacaaggta agtttcgttc ggaacaaatg
     6301 aaaaacctaa cacaggcggc atcaaaatca ggggtaattt gtatggattg aatataatct
     6361 gacggaacgc tttccaacca caccgtttgc caaatcccgg actgcgggct gtaccaaatt
     6421 ccgccgggct tccaacgctg tttcccgcga gtattccatg aaaaatctgt agtgtcttgt
     6481 acttttaccg ttaatatatt tctgtcattt ttctgccatg ccatggttat atcgagtgaa
     6541 aaaggcgtaa atccgcctaa atgagaaccg acttcaattc cgttaagata taccgtcgct
     6601 ttttgatcaa ccgctctgaa atgcagcagc aggtgatcaa gcaaaaaatc atcgggaagg
     6661 acgatttctc ttcgatacca taaatattga tctttcgtca atatgcgatt gacgccgctc
     6721 agttccgatt cgggagaaaa cggaacaaga atatcaccgt caaactgagc cggttgttcg
     6781 gatgattctg ttatcgcgta gttccaaaaa ccgttcagta ttaaataatg atctcgtacc
     6841 atctgcggcc gcgggtattc gctcagaatg gcatccggat ttaggttatt cccccatctt
     6901 gttttcatgt tggtttttcc cgaggaaata gagtggaatt acgataaaac acaacacgaa
     6961 agcagaaaca agaaaaatat ccggagtagg aatattcttt gtctgcccga gttcttcata
     7021 gattcccccg ttagatttaa taagccatgc tccgatgtac ggtccgataa ccatcggcag
     7081 cataacggta aaaatcatcc gaacgccttg gaaaacacct accttcccgg gcggggtaaa
     7141 atctcgaata agcgcggaaa gagttgcgct aatcatcata tatcccgaca tcataatgat
     7201 cccccaaaaa gtcaccggaa tgtttcggcg cgagaaaaat agcccgacca aaccgatcag
     7261 cattgtgata atcgccggat aaatgacttc cattttgccg tatttatcga tcaccgggcc
     7321 cattaaaatg ctgatcaaag aagcgataat caaaatcgtt cctaaaatca atgcataatt
     7381 ctctataaat aagaattgtt gaatgtaaat aatcaaatac ggaaaaaata cttggaaagc
     7441 ggctccgaat atacaaaatg caatcatgat aatatacaaa tttttattct catgaaaaga
     7501 agcgggagta aatccataga acaattccgc aaaaaaatta ccctttttct tttcaaccgg
     7561 cacttcttta atgaaaaaca gagaaaggaa tcccaccgcc aatacaatcc cgccggctat
     7621 tctgaaaaaa agattccagt taccctgttg tacgatcgga tcaaagcagc caaatacgat
     7681 taacatagcg atcaagggga aagtggctaa tacagattca actttgccgc gattggtcgg
     7741 aacagtaata tccgtgacat aagcgttaaa tgccgcgtca ttggctgtgg aaccgaaaaa
     7801 agtcattata caatccagaa caacgattcc atatgcggcg acaacggaag cgttagcaaa
     7861 cgggtacagt gcctgaatat tttctcggtt tacaaaacta aacagaataa ttgaaattcc
     7921 ccataaaata taccccgccg atataaatac tttccgcttg ccgaatcgat cggataaatt
     7981 tcccatcaat agagtcgtta aagttgcgac aaccgcactg gcagaaacca tcaacgctat
     8041 ataatgagga tcggttgagg cggtttgata catgaataaa ttcaaataca tattctcgat
     8101 catccatgca agttgtccgg tcaacccgat aaaaatcaga gaaagccatt gttttcgtgt
     8161 tagattcatt ctttcattat tcctgtccat tcagatttca agcagactat taaacattgc
     8221 aattctattc tacttgattt aaagacagag ttttacggat cttcttcatc cggataaatc
     8281 cgatatttga acgaatcggg ataaaataaa aattaaggtt ttgatctaaa ttaaaaggag
     8341 aataaaaatg cccaccagaa aatcacaagc aatatgggaa ggttcactaa aagagggaaa
     8401 aggtaggatg atgctcggaa gcggtgcttt tgaaggaaaa tattcattcg gatcacggtt
     8461 cgaagaagaa cccggcacca atcccgaaga attgattgcg gctgcacatg ccggctgctt
     8521 tacgatgttt ttatcttccc ttttaaccga tgccggattt acaccacgga agattaccac
     8581 aactgccaaa gtcaatctcg ctgccggtcc ggagatttcg aaaatagaat tggagacgga
     8641 agcggatgtc cccggaatca gcgataaagt gtttcaggaa caagctctga tcgcaaagaa
     8701 aaattgtccg atttcaaaag cgttgtcggc ggttcccgaa ataacattaa gtgcaaaatt
     8761 agtttaatcc tgataaggaa taatcaaaaa agccggaggc ttccggcttt tttggttttt
     8821 tacgacagac tacattttga tttcgatatc cacacccgcc gggagattca acctcatcag
     8881 catgtcgata gtttttgaat ccggatccaa cacatcgatc attcgattgt gagtccgaat
     8941 ttcaaaatgt tcctgagaat ctttatcgat aaaagtcgac cggcgaacgg taaaccgttc
     9001 aattcgggtc ggaagcggta cggggcctac gacatgagcg ccactgcgtt cggcggtatc
     9061 aacaatgcgt tttgcggatt gatccaaaat ccggtgatca aacgccttca gacgaatacg
     9121 aatcttttgc tttgccattt tcttgctttc ttcccgttat ccttcgattt cagtaatcac
     9181 accggcgccg acggtcaacc cgccttcgcg a
//
LOCUS       DAOSMM010000073         8707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_8058_length_8707_cov_19.175451, whole genome shotgun sequence.
ACCESSION   DAOSMM010000073 DAOSMM010000000
VERSION     DAOSMM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8058_length_8707_cov_19.175451"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PK898_08915"
     CDS             complement(<1..485)
                     /locus_tag="PK898_08915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HOU20192.1"
                     /translation="MKPLAPLPVRQLLNRYHLTPSKGLGQNFLVDDEILSEIVRIAEI
                     PPDSTVLEIGPGLGGLTRYLAEAADDVVAVELDRRMIPPLKEVLKEYDHVRIVQGDIL
                     EIDPDSLIVDSAYSVVANIPYYITSAVIRHLLNAKTKPRRIVLTVQKEVAQRICAQPG
                     DL"
     gene            complement(482..1870)
                     /locus_tag="PK898_08920"
     CDS             complement(482..1870)
                     /locus_tag="PK898_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HOU20193.1"
                     /translation="MKSKTAVLCGLLLITIGVVLFWANARRNIKLIIDGKPRQVATYK
                     ITVGQILKETGINFNELDRIFPEINYWMTERYFLKVDHLKAIHLVSSGGQKDYSTFEN
                     LAGNILADGEVALYPGDQIRWNGTQIQPDFNLGSLKAINLEVVRGDPFELITDLGKQR
                     QQFYTDATTVGEALRQTAVRIPPDSIIVPAEDTPFIAGMTIRILEETPLVLVSEGGEI
                     PLTGYGTNAGELLASAGYPLQGLDICIPAEDQPLPADHHIKVIHVAEEVTLKAETIPN
                     EIEWVADESDPLDQYKTISEGKPGLKGTIQRKRTENGIAVEEQISPEKVLVEPVTEVR
                     SYGTQISIQTLSTPDGQIEYWRAVPVYATSYSPCRSGVDSCINGTASGRKVEKGVIAV
                     DGDWYAEFAGLTVYVPDYGFAVIGDTGRSPVADNRWIDLAYNDENFVPWSNQTTLYFL
                     TPVPASIPWILP"
     gene            complement(1927..1999)
                     /locus_tag="PK898_08925"
     tRNA            complement(1927..1999)
                     /locus_tag="PK898_08925"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1964..1966),aa:Phe,seq:gaa)
     gene            complement(2110..2400)
                     /locus_tag="PK898_08930"
     CDS             complement(2110..2400)
                     /locus_tag="PK898_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20194.1"
                     /translation="MSIFHKAYPSRYKAEVERLTETLIQIGQKEDFLSERPGGIFDQN
                     CRNIQAREIGNRLFEIGGIELMEDRIKKISKKDGKEIAAHLEACWRAIGGIF"
     gene            2577..2960
                     /locus_tag="PK898_08935"
     CDS             2577..2960
                     /locus_tag="PK898_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU20195.1"
                     /translation="MDRKKVIIAEKAPKAIGPYSAAISYGNFIFTSGQLGLIPESGEP
                     AGEDIKSQTRQALKNVQSVLEAAGTDLSHVLKTTVFLRDMDDFAAMNEIYAEFFTDEC
                     PARSAVQVAKLPRGLAVEIEVIAAI"
     gene            2965..3990
                     /locus_tag="PK898_08940"
     CDS             2965..3990
                     /locus_tag="PK898_08940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU20196.1"
                     /translation="MPTVSIIVPCLNEEKNITRLLDALYRQTYPISETEVIISDGNSQ
                     DRTLDKIREWHDAHPDLALQIVSNPKRIIPAALNSALEASSGIYIIRLDAHSIPAPDY
                     VERCVRLLEEGKAENVGGRWEIQPGADTWVARSIANAASSPLGAGDAKYRFSETASFV
                     DTVPYGAYKKETLTALGGYDESLPINEDYDLNVRIRRNGGKIWFDPDIRCIYFSRTTF
                     RGLAKQYWRYGYWKLRMLKKDPKSLRLRQLIPPLFVFSLLFFAIGSFFSHAFLNVLLL
                     ELIVYIAVLLFAGIRTAGKENDLIQLIGLPIALAIMHFSWGSGFIWSLIKLIFRSEQE
                     SETGSHE"
     gene            4075..5499
                     /locus_tag="PK898_08945"
     CDS             4075..5499
                     /locus_tag="PK898_08945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU20197.1"
                     /translation="MAEQFFRSPFQSRLSSKERKIILFLGDLLVTILALVISLVTWAK
                     GDDWLGLSPEFFAERPENWFYFLPILWLLLMAPIYDLRRASSVPTTLNFILIASLLAG
                     TLYLTIFFVAPPKALPRRGVASFLISCAVLSILWRLFYIRKFTLPKYLINTLIVGAGK
                     AGCRIAEIIRGINPSPFSVAGFIDDDPEKIGKEFFGYPVLGDSNSFYKIVEEHQIGQV
                     IMAISNRMNPELFDALTMAEEKGLVVKTMPNVYEELLKRIPVYLLGTDWMVRDFYDQA
                     HASIFFEITKRLIDIFGGFIGTVFFAILFPFISVAILIDSGKPVFYSQERLGLRGTPF
                     NILKFRTMVQDAEADGIPRPASYEDNRITRVGHFLRKSHLDEMPQFLNVLRGDLSLVG
                     PRAERKEIIEELQKEIPFYRGRLLVRPGITGWAQINYGYAAGAEQNAIKLEYDLYYIK
                     NRNLLLDFSILLQTFKTIFGLKGR"
     gene            5503..7395
                     /locus_tag="PK898_08950"
     CDS             5503..7395
                     /locus_tag="PK898_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOU20198.1"
                     /translation="MASRFKGSIRNRFIFLADLILIVVCVFCSYILRLELGYTFYKYL
                     PSAYWMIGVSLVIKPTVYYFFGLYRRLWNYASMNEMKLILIAVTCASAVVSAVMVILA
                     TANVFFGFPRLVLIIDWILSMIAVGGVRFSVRIMYDLDRKNSIKSREKMKRALIIGAG
                     DAGALVLKEIQKNPQLNVHPVGFIDDDPKKMNQSIYGVKVIGTVNDIRKIIDRYAITD
                     VFFAIPSAPGRVVRVVSDSCREKEVNFRTMPGIYELLGGKISISKLREVEITDLLRRD
                     PVKLDAQNIGSILTDKIVLVSGAGGSIGRELCNQIARWNPARIILLGHGENSIFETLL
                     DLHENYPALSLKPQIADIRDQDRLREIFKEFHPDIVFHAAAHKHVPLMEFNGPDAITN
                     NVLGTWNMAHIANENGVERFVMISTDKAVRPVNIMGATKRIAENIVLNESAKSAAEFT
                     VVRFGNVLGSRGSVVPIFQEQIKKGGPVTITHPEMKRYFMTIPEAVYLVLEAAGLATK
                     EETFVLNMGKQVRILELAEDLIRLSGLEPGKDIEIKFTGIRTGEKLSEDLWNEGQILE
                     PTLHPDIFKLERDDLMDDETLSNVVRRLLELAKEGDDSAIRKYLNEVIPDANISERAI
                     SDIFTF"
     gene            7435..8478
                     /locus_tag="PK898_08955"
     CDS             7435..8478
                     /locus_tag="PK898_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOU20199.1"
                     /translation="MAQISASQWKLTIEAQKDASLLQTAEWGSVKAAFGWSPAYVMNE
                     AGGAMILFRNLPRVALGATMAYIPRGPVIFHNEPQKINDLWEEIHRLCRARHAVFLRV
                     EPDFWQNTAEAEFLRNSMQGFENAFSTVQPPRTIIVPLTGSEEDRLARMNQKTRYNIR
                     LAQKKDLVIREENNVSAFMELMAKTGERDHFHVHSAEYYQHCYNVFKESGKAWNLVAY
                     YHDKPLGVLMLFIHGNRGYYLYGASSDEERNRMPNHLLQWTAMNICAANGCTEYDLWG
                     VPDEEPETLEAQFQERSDGLWSVYRFKRGFGGEIKRTMGSFDFPYHPLFYRLAATFYR
                     RKKAQQASGGPQN"
     gene            8475..>8707
                     /locus_tag="PK898_08960"
     CDS             8475..>8707
                     /locus_tag="PK898_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOU20200.1"
                     /translation="MNSVTLSTPPDHWNEEIASLPGASILQTSQWAEIKAGGGWTPLF
                     QKWTATDGELRAAALILRKKIFLQYEFWYIPRGP"
BASE COUNT         2259 a         2144 c         2044 g         2260 t
ORIGIN      
        1 agatccccgg gttgcgcgca aatacgctgc gcaacctctt tctgaaccgt caggacaata
       61 cggcgcggct tcgttttggc attcaataaa tggcgaatca ctgcagaggt gatgtaatag
      121 ggaatattcg caaccacgga atatgccgaa tcgacaatga gagaatccgg atcgatttcc
      181 aaaatatcgc cttggacaat ccggacatga tcgtactctt ttaaaacttc ttttaacggc
      241 gggatcattc gccggtcaag ttccacagcg accacgtcat cggccgcctc ggccaaatat
      301 cgggtcagtc cacccaaacc gggaccaatt tccaaaacag tcgaatcggg gggaatttcc
      361 gcgattcgaa cgatttcgga taagatttca tcatcaacca aaaaattctg tcccaagcct
      421 ttgctcggag tcagatgata acggttcagt aactgtcgga caggtaaggg agcgagtggt
      481 ttcatggcaa aatccatgga attgaggcgg gtacgggtgt caggaaatag agagtcgttt
      541 gattactcca aggtacgaaa ttttcatcat tataagccaa atcgatccaa cggttatcag
      601 cgaccggtga acggccggtg tcgccgatga cggcaaaccc gtaatcgggt acataaaccg
      661 tcagtcctgc gaattcggca taccaatcgc cgtcgacagc gatcacccct ttttcaacct
      721 tccgacccga tgccgtgcca ttaatacaag agtcgacacc actgcgacag ggagaatatg
      781 aagtcgcata aacgggaacc gcccgccaat actcaatctg tccgtccggc gtcgaaagtg
      841 tttggatgga tatttgggtt ccgtaggaac gcacttccgt taccggctca accaacacct
      901 tttcggggct gatctgttct tcgaccgcta ttccgttttc cgttcgcttc cgttgaatcg
      961 tcccttttaa accgggcttt ccttcggaga ttgtcttgta ttgatccaaa ggatcggatt
     1021 catccgctac ccattcgatt tcattcggga tcgtctccgc tttgagagtc acttcctctg
     1081 ccacatgaat cacttttata tgatgatccg cgggaagggg ctgatcttcg gccgggatgc
     1141 agatatccaa cccttgcagg ggatatcccg ctgacgcgag caattcgccg gcatttgttc
     1201 cgtaccccgt cagaggaatc tcgccacctt ccgagaccaa aacaagcggg gtttcttcca
     1261 gtatccgaat cgtcataccg gcgatgaacg gcgtatcctc cgcagggacg atgatcgaat
     1321 cgggaggaat ccgaaccgcc gtttgccgaa gcgcttcacc gacggttgtc gcatcggtat
     1381 agaattgctg ccgttgtttt cccaaatccg taatcagttc aaagggatct ccgcgaacca
     1441 cttccaaatt gattgccttc aaagaaccca gattaaaatc cggctgtatt tgggttccgt
     1501 tccatcgtat ctgatccccc ggataaaggg caacctctcc gtccgccaag atattcccgg
     1561 ccaagttttc aaaagttgag tagtcttttt gcccgccgga agaaacaaga tgaatcgcct
     1621 taagatggtc gactttcaga aaataccttt cggtcatcca ataattgatt tccgggaaga
     1681 ttcggtcgag ttcgttaaaa ttgattccgg tctccttcaa aatttgacct accgtgattt
     1741 tatacgttgc cacttgccgg ggctttccgt caataatcaa cttaatattc cgtcgggcgt
     1801 tcgcccaaaa aaggacaacg ccgattgtta tcaagagtaa accgcacaga accgccgttt
     1861 tcgatttcat cccgcttccg aaattaattg taaaacaaaa agacccgcca aagcgggtcc
     1921 tcgctgtgcc gagacacaga atcgaactgt ggacactaca attttcagtc gtatgctcta
     1981 ccaactgagc tatctcggca tcgtttccat tcaaacgacg gtaccaattt tagcggctta
     2041 tcgaacgact gtcaagcatt tatgaaaaat catgtgatcg attcaaacaa ttgaaatcga
     2101 gccgaattat caaaatattc ctccgattgc ccgccaacaa gcttccaaat gagcggcaat
     2161 ttctttcccg tcttttttgg aaattttctt tattcgatct tccattaatt caatcccgcc
     2221 gatttcaaat aaccgattcc caatctcacg cgcctgaata ttccggcaat tctgatcaaa
     2281 tatgccaccc ggcctttcag aaaggaaatc ttctttttga ccgatttgaa tcagtgtttc
     2341 ggttaacctt tcaacttcgg ctttatatcg agaagggtaa gctttatgaa aaatactcat
     2401 tttttccttt ctttctgatt ttctataaaa aaatccagaa caaattgatg aaacctttta
     2461 aaacttttga aaactttcac acccatttcg agaattttcc ccatggtaaa attgctgtat
     2521 aaataatctt atcagattta ctcggatctt gataatgtta aaggaagagg aagcaaatgg
     2581 atcggaaaaa agtaattatc gccgaaaaag caccgaaagc gatcggaccc tattcggcag
     2641 caatctccta cggaaatttc atttttacat cgggtcaact cggtttgatt ccggaaagcg
     2701 gtgaaccggc cggtgaggat ataaagagtc agacccgtca ggctctaaaa aatgttcagt
     2761 cggttctgga agcagccgga acggacttat cccatgttct gaagacgacg gttttcttgc
     2821 gggatatgga tgattttgcc gccatgaatg agatttatgc tgaatttttc acagacgaat
     2881 gccccgccag atcagccgtc caagtcgcaa aactaccgag agggctcgca gtcgaaatcg
     2941 aagtgatcgc tgcgatttaa accgatgccg actgtttcga ttatcgttcc gtgcctgaat
     3001 gaagaaaaaa acatcacccg tctgctcgat gccttgtatc gtcagactta tccgatttca
     3061 gaaacggaag tcattatttc cgacggaaat tctcaagacc ggacactgga taaaatccgg
     3121 gaatggcatg atgcccatcc ggacttagcg cttcagattg tatccaatcc caagcggatt
     3181 attcccgccg cgctcaattc cgcattggag gcgtcaagcg gaatctatat catccgtctg
     3241 gatgctcatt ccattccggc tccggactat gtcgaacgtt gtgttcgttt gttagaggaa
     3301 ggaaaagcgg agaatgtggg aggccgctgg gaaatacagc ccggagcgga tacctgggtc
     3361 gcccgatcta ttgccaacgc cgcttcttct ccgcttggcg caggagatgc caagtatcgt
     3421 ttttccgaaa cggcatcctt tgttgatacg gtaccttacg gagcttataa aaaagaaact
     3481 cttacggcac tcgggggata tgatgaatct ctgccgatca acgaagatta tgatctcaac
     3541 gttcggatcc gtcgcaacgg cggaaagatt tggtttgatc ccgacattcg ctgcatttat
     3601 ttttcgcgga ccactttccg cggtttagcc aaacaatatt ggcgatacgg atattggaaa
     3661 ctcagaatgc tgaaaaaaga tcccaaaagc ctccgtttac gccaactgat tccgccgctc
     3721 ttcgttttca gtctgttgtt ttttgcgatc ggcagttttt tttcgcatgc ctttttgaat
     3781 gtgttattat tggagctaat cgtttacatt gccgtcttgc tttttgccgg aattaggact
     3841 gcgggcaaag aaaacgatct gatacaactc ataggattac ccattgcttt ggcaattatg
     3901 catttcagct gggggagcgg atttatctgg agtctaataa aactgatttt taggagtgaa
     3961 caagaatccg aaactggctc tcatgagtag aaagttgttc ccgctttcaa aagaattcgc
     4021 acagaacggt aacatcaatc ataattgagc aaaacgtttc cgacatgaag aaaaatggct
     4081 gaacaattct ttcgaagtcc atttcaatcc cgcctgtcat ccaaggaacg taaaattatc
     4141 ctgttcctcg gcgatttact ggtcacaata ttggcgctgg tcatctctct ggtcacatgg
     4201 gcgaaaggcg atgactggtt agggctctct cccgaatttt ttgccgaaag accggagaat
     4261 tggttttatt ttcttccgat cctttggtta ctattaatgg cgccgatata tgatctgcgc
     4321 cgggcaagtt cggttccgac cacattaaat ttcattctga tcgccagttt acttgccgga
     4381 acgctctacc tgacgatctt cttcgtcgct ccgccaaaag cgctaccccg ccgcggggtc
     4441 gcctcttttc tcatcagttg tgccgttctt tccatccttt ggcgactttt ttatatccgg
     4501 aaatttaccc tgcccaaata tctgatcaat acgttgatcg tcggcgcggg aaaagccggc
     4561 tgtcgcatcg cggaaattat caggggcatc aacccgtctc cgttttcggt cgccggattt
     4621 atcgacgatg atcccgaaaa aatcggaaag gagttcttcg gctatcccgt gctgggggat
     4681 tcaaattcat tttataaaat cgtggaagaa catcagatcg gtcaggtgat catggcgatc
     4741 agcaaccgaa tgaatccgga attatttgat gccttgacaa tggcggaaga aaaagggttg
     4801 gtcgtcaaaa ccatgcccaa tgtttacgaa gaactgctca aaaggatacc ggtttatctg
     4861 ctcggaaccg actggatggt gcgtgacttc tatgaccagg cacatgccag catctttttt
     4921 gaaatcacca agcgcctgat cgatatcttc ggcggattca tcggcacggt tttttttgcg
     4981 attctctttc cgttcatctc cgtcgccatt ttgatcgatt ccggcaagcc tgttttttat
     5041 tctcaggaaa ggctcgggct gcggggcaca ccgttcaata ttctgaagtt tcgtaccatg
     5101 gttcaggatg ccgaagcgga cggtatcccg cgaccggctt cttacgagga taaccgcatc
     5161 acccgggtcg gtcactttct gcgcaaaagt catctggacg agatgccgca attcctaaac
     5221 gttttaagag gtgacctcag cctggtggga ccgcgcgccg aacgaaaaga aatcatcgaa
     5281 gaactgcaaa aagaaattcc tttttatcgg ggcagactgc tggttcgacc cggcatcacc
     5341 ggttgggcgc agattaatta cgggtatgcc gccggagccg aacaaaatgc gattaaactg
     5401 gaatatgatt tgtattatat taaaaatcgc aacctgttac tggacttttc catccttctg
     5461 cagactttta agacgatttt cggtttgaaa ggaagataat ctttggcatc ccgctttaaa
     5521 ggcagcattc gaaaccgttt tatttttctg gcagatctga ttttaattgt tgtatgcgta
     5581 ttctgcagtt acatcctgcg tttagagtta ggttacacct tttataaata cttgccttcc
     5641 gcttattgga tgatcggcgt ttccctggtt atcaagccga cggtctatta cttcttcggg
     5701 ctgtatcgca ggctttggaa ttacgccagc atgaatgaga tgaaactgat tctgattgcg
     5761 gtcacctgtg catcggcggt tgtttcggcg gtcatggtga tcctggcgac agccaacgtc
     5821 ttcttcggat tcccgcgatt ggttctgatc atcgactgga tcctctccat gatcgcagtc
     5881 ggcggcgtcc gtttttcggt cagaatcatg tatgatttgg accggaagaa ttcgattaaa
     5941 tccagagaaa aaatgaagcg cgcactgatt atcggagcgg gggatgcggg cgcattggtc
     6001 ctgaaagaaa tacaaaagaa cccgcagtta aacgttcacc cggtcggctt tatcgacgat
     6061 gatcccaaaa aaatgaatca atccatctat ggtgtcaaag tcatcggaac cgtcaacgac
     6121 attcggaaaa tcatcgaccg gtacgccatc actgatgttt tctttgcgat tcccagcgcc
     6181 cccggccgtg tcgtgcgggt ggtttccgac agctgccgtg aaaaagaggt aaatttccgc
     6241 accatgccgg gaatttacga gcttttgggc ggcaagattt ccatttcaaa attgagagaa
     6301 gtcgaaatta ccgacctttt gcgtcgggat ccggttaagc tggacgccca aaatatcggt
     6361 tcgattctga ccgacaaaat agtcctggta agcggagcgg gcggttcgat cggtcgggaa
     6421 ctttgcaatc aaatcgcgcg ctggaatccg gcccgcatta tcctgttggg gcacggtgaa
     6481 aacagcattt tcgaaaccct tctggattta cacgaaaatt atccggcttt atcgctcaaa
     6541 ccacagatcg cggatattcg cgatcaagat cgtttgcgag agatctttaa agaatttcac
     6601 cccgacattg tgttccatgc cgcggctcac aaacatgtcc cgttgatgga attcaacggt
     6661 cccgatgcga taaccaacaa cgttctcggc acttggaaca tggcgcatat cgccaacgaa
     6721 aacggcgtgg aacgcttcgt aatgatctcc accgataaag ctgtcaggcc ggtgaacatc
     6781 atgggcgcca ccaaacggat cgccgaaaac atcgtattga acgaatcggc caaaagcgct
     6841 gccgaattta ctgttgtccg cttcggaaat gtgttgggca gccgcgggag cgtggttccc
     6901 atcttccaag aacagatcaa aaaaggcgga ccggtgacca tcacccatcc cgaaatgaag
     6961 cgctacttta tgaccattcc ggaagcggta tatctcgttt tggaagccgc cggactcgcc
     7021 accaaagaag aaaccttcgt cttgaatatg gggaaacaag tccggatact ggagcttgcc
     7081 gaagatttga tccgcctttc cgggttggaa ccggggaaag acatcgaaat taaattcacc
     7141 ggcatccgta ccggtgaaaa attgagtgaa gatctttgga acgaagggca gattttggaa
     7201 ccgacgcttc acccggatat tttcaaattg gaacgcgatg atctgatgga tgatgagact
     7261 ctctccaatg tcgtacgccg tctgttggaa ctggcaaaag aaggcgatga ttccgcaatt
     7321 cgcaaatatc tgaacgaagt gatccccgat gcaaatatca gtgaaagagc aatatcagat
     7381 attttcactt tttagatccg ttcatccgct ttgttcggta caataactga caaaatggca
     7441 cagatttcgg cttcccaatg gaaattaacg attgaagcac aaaaagacgc ctcgctgctg
     7501 cagaccgcgg aatggggatc ggtcaaagcg gcattcggtt ggtcgcccgc ctatgtgatg
     7561 aacgaagcgg gcggggcgat gatcctcttt cggaacttac cgcgagtagc actgggcgcc
     7621 acaatggctt acatcccgag aggcccggtc atttttcaca atgagccgca aaagatcaac
     7681 gacttgtggg aagaaatcca tcgcttatgc cgggcgagac atgccgtttt tttgcgtgtc
     7741 gaaccggatt tctggcagaa taccgcggaa gccgaattct tgcggaattc gatgcagggg
     7801 tttgaaaatg ccttttccac cgttcaaccg ccgcggacaa tcatcgtccc tttaaccggc
     7861 agcgaagagg accggcttgc ccgcatgaat cagaaaaccc gctacaacat ccgtttggcc
     7921 caaaagaaag acctcgtgat tcgagaggaa aacaatgttt ccgcgtttat ggagctgatg
     7981 gcgaaaacgg gggaaagaga tcactttcat gttcattccg ccgagtatta ccaacattgc
     8041 tataacgttt tcaaagaaag cggtaaagca tggaatttgg tggcctatta tcacgacaaa
     8101 ccattgggtg tccttatgct tttcatccac ggaaaccgcg gctattacct ctacggcgcc
     8161 tcttctgacg aagaacggaa tcgaatgccc aatcaccttc ttcagtggac agccatgaac
     8221 atctgtgcgg cgaacggttg cacggaatat gatttgtggg gagttccgga cgaggaaccc
     8281 gaaacgctcg aagcccaatt ccaagaaaga tccgacggat tatggagcgt ttatcgtttt
     8341 aaacgcggtt tcggcggtga aatcaagcgc actatggggt cgttcgattt cccgtatcac
     8401 cccctgttct atcgtctggc ggcaactttt tatcggcgaa agaaagcgca acaagcatcc
     8461 gggggaccgc aaaattgaac tccgtcacac tctcaacccc acccgatcat tggaacgagg
     8521 aaatcgcttc tctgcccggc gcatcgatat tgcaaacaag ccaatgggca gaaattaaag
     8581 ccggcggtgg ttggacgccg ctcttccaaa aatggacggc aaccgacgga gagttacgtg
     8641 ctgccgcatt aatcctgcga aaaaagatct tccttcaata tgaattctgg tacattccgc
     8701 gcggacc
//
LOCUS       DAOSMM010000074         8289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_8578_length_8289_cov_15.030726, whole genome shotgun sequence.
ACCESSION   DAOSMM010000074 DAOSMM010000000
VERSION     DAOSMM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8578_length_8289_cov_15.030726"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1487
                     /locus_tag="PK898_08965"
     CDS             195..1487
                     /locus_tag="PK898_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20201.1"
                     /translation="MKASKNKFIRLPGIGFRWIIPVLFILLLNSEIAFSQFDGKIESF
                     EFIYRQDFSGEPDDWNLEPDTGKWGSVDYSFTDETYRWEMNFKKDVLNYQTPDPIIYL
                     PEDGYRIGVETYFEDTCDDCASGLLYNLQDESNFYYVRLYGDGYIQVYAKEDNEWEAL
                     SEAVQSEYFIPSESNRITVINDGDNFEIQLNDYPIFNFTDSRFHGGTFGLVAEAPKGT
                     QLAVRFDNIDVMKKGSTQVVDLEEETVTETEEPALEEETVAETEEPDWENESPYRINK
                     VTAPMNDFYPPDEESVLTDYTLDISIPITFWKEERGNLFCPKESNENACVFIIQHFDR
                     WGSPSEAADDILGKYAEQVEDFEIFDEEETFTKDGFYAYRVGTYFTIDGKNYEGSHLF
                     VQVQNVVFQIMSYGTRGTIDKYREDLKQITDSFSFEYF"
     gene            1790..3070
                     /locus_tag="PK898_08970"
     CDS             1790..3070
                     /locus_tag="PK898_08970"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOU20202.1"
                     /translation="MTTCAIVGINWGDEGKGRMVDLVAMDYDVVIRYQGGNNAGHTVV
                     NEYGKFALNLVPSGIFRREVVNVLGSGTVVNPEHLCGEIDKLRKAGITITPENFIISD
                     RSVIVFPYHIEQDSLEESRLADKKYGSTKRGIAPVYGDKYLKKAIHMGELRYPDKLKA
                     HLDDVLKWKNLTITKIYEAEPFQLDELMDWVMEFGGRLLPHITDTGDFLRKAYAEGKN
                     ILYEAQLGALRDVDYGIYPYTSSSNPLASYAPIGSGAPDIKVDKVIGVVKAFSTCVGE
                     GPFIAEWFGDDAKKLREAGGEYGAATGRPRRVGPIDLPATRYGVKVQGATEIAFTKLD
                     VLSYMDEIPVCTAYDINGSITRDFPFHSLLDDAKPVFETMPGWKCDITGARKYDELPG
                     EARRYIEYVENEIHCPMTYISVGPGRDEIILRKA"
     gene            3067..4479
                     /gene="purB"
                     /locus_tag="PK898_08975"
     CDS             3067..4479
                     /gene="purB"
                     /locus_tag="PK898_08975"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU20203.1"
                     /translation="MKERYESPLAGRYASDYMIELFSEKNRHILWRKLWVELARAEHE
                     LGLPITSEQVKELEAHITDIDYAVVSAKEKEIRHDVMAHIYAYGEVCPKAKGIIHLGA
                     TSCYVTDNADLILYRQALSYIRNELWHVISILKDFALRYKAMPTLGYTHLQPAQLVTV
                     GKRACLWIQDLLNDAAELDFVLSQIRFLGCRGTTGTEASFMELFKGDSAKIDEMNRRI
                     AAAFGFQSLFPVCGQTYPRKLDSQILNVLSAVCQSCYKFANDLRLLQHLQQIEEPHEA
                     EQVGSSAMAYKRNPMRSERLCSLARFVMVDALNAPLTASVQWFERTLDDSANRRITLP
                     EGFLATDAVLRLMSNISRGLIVNEKIIERDVRQFLPFIATENLLMASVEKGGDRQISH
                     EIIRKHSLEVADRIKNGESGNLFELLANDPDFNLSAEEINSALNPQDFIGRCVEQVEN
                     FLNSFEIQYSSDFLEEEITL"
     gene            4522..5397
                     /locus_tag="PK898_08980"
     CDS             4522..5397
                     /locus_tag="PK898_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU20204.1"
                     /translation="MKKKLTEKSSWIWSIVILGGIPLVIYILWRYLVSKNAFDKTLLI
                     HPYEIGTAKINRPVTLVLLTDLHNNFFGTEQNELVKAIQTVNPDVILMAGDMIDRKFP
                     LDGFLALIRNLPESVPAFFVTGNNDFWKGYPAGIKTILRDHGVNVLEGECVLINVKNQ
                     MIDVCGIDDPKIGKKIYHAQISSAGEQKNSGCYSILLMHRPDRRHLNEVLPYHYDLIL
                     SGHAHGGQWRIPGLVNGLIAPDQGIFPRYTGGLYQHGETNQIVSRGLAKNSSLIPRIF
                     NRPELVVIQLLPDKR"
     gene            5552..6694
                     /locus_tag="PK898_08985"
     CDS             5552..6694
                     /locus_tag="PK898_08985"
                     /inference="COORDINATES: protein motif:HMM:NF005593.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOU20205.1"
                     /translation="MKIQPFKVEEWMNAYEDSAIYNIAETCVDPLSIRELFELTATDI
                     EDFTERIFGRPLTYGAITGSHSFKTGIGKLYRSLKPDNILSTHGAAGANHLVFYALTE
                     PGDHVISVMPTYQQLYSIPESFGAEVQILKLRRENNYLPDLAELKEMIRPDTKLICLN
                     NPNNPTGALIPNEMLREVVEMAEKVGAYVLCDEVYRGLNQNHEYTESVADLYERGISV
                     GSMSKIFSLSGLRLGWIATHNQEVIKACFSHRDYSLISCGILDEAVASEALKNGSAVF
                     NRNLTRIRENLALLDSWIREQAHVSYVKPQAGTTALIHYDFDIPSYDFCKQLLDKTGV
                     FLTPGACFDEEYCFRIGYACATQTLKDGLQKLTEFTGGLSENSAIS"
     gene            complement(6698..7951)
                     /locus_tag="PK898_08990"
     CDS             complement(6698..7951)
                     /locus_tag="PK898_08990"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU20206.1"
                     /translation="MENSIKKYNNPFYSLGYPNFRLYWVGMCISNIGTWMQNIALPWL
                     ALTTTNNPFLVSLVSASQFIPTLLLSLFSGAILDRVNKKRVLIITQVGFLLVSLSFVI
                     MIYLDWLNFGLMIVLSFIYGIFNAFDSPCRQSFIYELVEQKEDLPNAVALNSMAFNAA
                     RVIGPSIAGIIMTKFGASSCFLINSISFLFVLVSLFFIKTHPEQVENPIGKNIIENIK
                     DGLIYIKNSSVLSLSLIILLIVATFIPNFNVLNSAFAKYVLNGNETTFGYLMSFMGIG
                     AFFGALLFATASRRGPSRKILYTVSILCGIFTFLCGTVNNLFTAGIFHILNGFCYILT
                     LSNVNSTLQINTENRYRGRVMSIYSLFLNGTTPLGNLYAGFFAERFNPRVAFYSCGLA
                     GLIFIGIITLLRPNSIKEYLQIKKE"
BASE COUNT         2431 a         1586 c         1974 g         2298 t
ORIGIN      
        1 gacctttttt tgccgctgat cgtccggtaa tacaataatt ttgcctattt tctttttctt
       61 atcccaagat ataatatgta taaacaaaca tattcagcgt ttttttatta ggataattta
      121 agacaggtaa tcgatcggta aaataattgt taatttctga aaaaggcttc cgtatatcca
      181 attaaggagg aatgatgaaa gcttccaaaa ataagtttat tcgtctaccg gggatcggat
      241 ttcggtggat tataccggtg ttattcattt tacttctcaa tagcgagatt gcattttcac
      301 agttcgacgg aaaaatcgaa tccttcgaat ttatataccg gcaggatttt tcgggagaac
      361 cggatgattg gaatcttgaa ccggacacgg ggaaatgggg atcggtagat tattccttta
      421 cggatgagac atatcgctgg gaaatgaact ttaagaaaga tgtactgaat tatcaaactc
      481 ccgatccgat catctattta cccgaagatg gctatcgaat cggtgtggaa acttatttcg
      541 aggatacctg cgatgactgc gcttccggat tattgtacaa cctgcaggat gaatcgaatt
      601 tttattatgt aagattatac ggagacggat atatccaagt ctatgccaaa gaagacaatg
      661 aatgggaggc gctcagcgaa gcggttcaaa gcgaatattt tattccttcc gaaagtaacc
      721 gtatcacggt aattaacgac ggggataatt ttgaaattca attaaatgat tatcccatat
      781 ttaattttac ggacagccgt tttcacggcg ggactttcgg tttggtagcc gaagctccga
      841 aaggaacgca attagcggtc cgatttgata atatcgatgt aatgaagaaa ggctcgaccc
      901 aggttgtgga tttggaagaa gaaactgtga ccgagaccga agaaccggct ttggaagagg
      961 aaacagtagc cgagacggag gaaccggatt gggaaaacga gtctccttat cgaatcaata
     1021 aagtgaccgc tccgatgaat gatttttatc ctccggatga ggaatcggtt ttgacagact
     1081 acaccttaga tatttctatt ccgattactt tttggaaaga agaaagagga aatctgtttt
     1141 gtccgaaaga atccaatgaa aacgcctgtg tatttatcat tcaacatttc gatagatggg
     1201 gatcgccttc agaagcggca gatgatattc tcggaaaata tgctgaacaa gtggaagact
     1261 ttgagatctt tgatgaggaa gagactttta cgaaagacgg gttctatgct tatcgggtcg
     1321 gaacttattt cacgatcgac gggaaaaatt atgaaggcag tcatctgttt gttcaagtgc
     1381 agaacgtagt cttccagatt atgtcttacg gaaccagagg gaccattgat aaatatcgag
     1441 aagatctgaa acaaataacg gatagtttct cttttgagta cttttaactt aaaaatactt
     1501 ttttgccgct aaggaaatcc aacttttcgg tagattgatc gattgaaaaa tccgttttcg
     1561 cttgttgaaa acggattttt ttattcggaa gtcggaagaa ctttcggttg cgttatttga
     1621 gaaatacttg atcagaaatg aaatataaga aaatatatta tgtagactta tatagaatta
     1681 caattgtgtc tatatatttt gtaattcaaa agttatgcct atgaaaagat atataattga
     1741 tttgttacga aagaattcat aaaaaaaatc ggctgcgggg aggcatacca tgacgacatg
     1801 cgcaattgtt gggattaatt ggggagatga gggaaaaggc cggatggtag atctggttgc
     1861 catggattac gatgttgtga ttcgttatca aggaggcaat aatgccggac ataccgtcgt
     1921 taatgaatac ggaaagtttg cattaaatct cgtcccctcc ggtatttttc ggagagaagt
     1981 tgtaaatgtg ctcggaagcg ggacggtagt taatccggaa catctttgcg gcgaaataga
     2041 taaattgcga aaagccggaa tcaccattac ccccgagaat ttcattatca gtgaccgttc
     2101 ggtgattgtt tttccgtacc atatcgaaca ggattcactg gaagaatcaa gattggcaga
     2161 taaaaaatat ggatcgacca aacgaggaat tgcgccggta tatggcgata aatatttaaa
     2221 gaaagcgatc catatgggcg aattgcgtta tcccgataaa ttgaaagccc atttggatga
     2281 tgttctgaaa tggaaaaatt taaccataac caaaatttat gaagcggaac cgtttcaatt
     2341 ggacgaactg atggactggg tgatggaatt cggcgggaga ttacttcctc atattaccga
     2401 caccggggac tttttacgga aggcatacgc ggaaggaaag aatattcttt acgaagcgca
     2461 gttgggtgcg ttgagggatg tggactacgg aatatatccc tatacttctt cttccaaccc
     2521 gttggcatcc tatgctccga tcggatccgg tgcaccggat ataaaagtcg ataaagtgat
     2581 cggggtggtc aaagcctttt cgacctgcgt cggggaagga ccttttattg cggaatggtt
     2641 cggtgacgat gccaaaaagt tgcgggaagc gggcggagaa tatggtgcgg ctaccggcag
     2701 gccgcgccgg gttgggccga tcgatctgcc tgcgactcgt tacggagtca aggttcaggg
     2761 tgcgaccgag atcgcgttta cgaaactgga tgtgctttct tatatggatg agattcctgt
     2821 ttgtacggca tacgatatca acgggagtat cacgagagat tttccttttc attcgcttct
     2881 ggatgatgcg aaaccggttt ttgaaacgat gccgggttgg aaatgcgata ttacgggtgc
     2941 gagaaaatac gatgaactgc ccggtgaagc ccgtcgttat attgaatatg tcgaaaatga
     3001 aattcattgt ccgatgactt atatctccgt cggtcccggc agagatgaaa tcattttgag
     3061 gaaggcatga aagaacggta cgaaagcccc cttgcgggac gatatgcatc ggattatatg
     3121 atcgaattat tttccgaaaa gaaccggcat atcctttggc gcaaattatg ggtggagttg
     3181 gcgagagcgg aacacgagct cggtcttccg attacttcag agcaggtcaa agaattggaa
     3241 gctcatatca ccgatatcga ttatgcggtt gtctccgcaa aagaaaaaga gattcgtcat
     3301 gatgtgatgg ctcacatata tgcttacggc gaagtctgcc ccaaagcaaa ggggattatt
     3361 catctcggag cgacaagttg ttacgtgacc gataacgcgg atttgatcct ctatcgccaa
     3421 gcgttaagtt atatccggaa tgaattatgg catgtcatct cgattttaaa agatttcgca
     3481 ctgcgatata aggcgatgcc gactctcggt tacactcatt tacaaccggc gcaattggta
     3541 acggtcggga agagagcttg tctatggatt caggatttgc tgaatgacgc agccgaactg
     3601 gattttgttc tcagtcaaat ccgttttctc ggatgtcggg gaaccacggg caccgaagcc
     3661 agttttatgg agctgtttaa aggggactcg gcaaaaatcg acgaaatgaa ccggcgaatt
     3721 gcggctgcgt ttggctttca aagtcttttt ccggtatgcg gacagactta cccacggaaa
     3781 ttggattcac agattttgaa tgttttgagc gccgtctgtc aaagttgtta taaatttgcc
     3841 aatgatttac ggcttttaca gcatttacag cagattgagg aaccgcatga agccgagcaa
     3901 gtcggttcct ctgctatggc atataaaaga aatcccatgc gcagcgagcg tttgtgttca
     3961 ctcgcccgct tcgtaatggt ggatgcattg aatgcaccgc tgaccgcgtc ggttcaatgg
     4021 ttcgaaagga cattggatga ttccgccaat cggcgcatta ctttgcccga aggatttttg
     4081 gcgaccgatg ctgttttacg tcttatgagt aatatcagtc gcgggctgat tgtcaatgaa
     4141 aaaattatcg aacgtgatgt gcggcaattt cttccgttta tcgccaccga aaatttgttg
     4201 atggcttcgg ttgaaaaagg cggcgaccgg cagatctcgc atgaaattat ccgaaaacac
     4261 tcgttggaag tggccgatcg gattaaaaac ggagaaagcg ggaatctatt tgagctgctg
     4321 gcaaatgacc ccgattttaa tttaagcgcc gaagaaataa attcggcttt aaatccacag
     4381 gattttatcg gacgatgcgt ggaacaagtt gagaattttt tgaactcctt tgaaattcaa
     4441 tattcttcag actttttgga agaggaaatc accctttgat ctttttttga taggggttaa
     4501 aacagggggg agcggtcttc attgaaaaag aaactaaccg aaaaatcctc atggatttgg
     4561 tcaatcgtaa ttttgggagg aattccgctt gtaatttata ttctatggcg atatcttgtc
     4621 agtaaaaatg cctttgataa aacacttctg attcatcctt atgaaatagg caccgcgaaa
     4681 atcaatcgac ctgtcactct tgttttactg acggatttgc acaataattt tttcgggact
     4741 gagcagaatg aattggtaaa agccatccaa acggttaacc ccgatgtgat tctgatggcc
     4801 ggcgatatga tcgatagaaa atttccgtta gacggatttt tagcactaat tcggaatttg
     4861 cccgagtcgg taccggcttt ttttgtaaca gggaacaatg atttttggaa aggttatccc
     4921 gccggaatta aaacgatctt acgcgatcat ggggttaacg tactggaagg ggagtgcgta
     4981 ttgatcaatg tgaagaatca aatgatcgac gtttgcggaa ttgatgatcc gaagatcggg
     5041 aaaaagattt atcatgccca aatttcttcc gccggagaac aaaaaaattc cggctgctat
     5101 tcgattcttc tgatgcatcg gcctgatcgg cgccacctta acgaggttct gccttatcac
     5161 tatgacctta ttttatcggg acatgctcat ggcggtcagt ggcgcatccc gggattagtt
     5221 aacggattga tcgcacccga tcagggaatc tttcccaggt atacgggagg cctctatcaa
     5281 catggagaga cgaaccaaat cgtcagccgc gggttggcaa aaaacagcag cttaattccc
     5341 cgaattttca atcgacccga attggttgtg attcagcttc tgccggataa acgctgaatc
     5401 tcagtaaggg atctttccgg cccgccggcg agaagaattg cagatcgcca tcaaatccga
     5461 tactatcaag gtttatcgcg taatatccga acagagatta tcgacatttg tcgaaatgtt
     5521 tggtaagagt agttaaccgg agaggacttt tatgaaaatt cagcctttta aagtcgaaga
     5581 atggatgaat gcttatgaag attccgcgat atataatatc gccgaaactt gcgtcgatcc
     5641 gttatcgatt cgagaacttt ttgaattaac cgcaaccgat atcgaagatt ttaccgaaag
     5701 aattttcggg cgaccgctta cttacggcgc cattaccgga tcacactctt ttaagaccgg
     5761 aattggtaag ttatatcgga gtttgaagcc ggataacatc ctctcaacac acggagccgc
     5821 cggggccaat catctggtat tttatgcttt gacggaaccc ggagatcatg tgatttcggt
     5881 catgcccact tatcagcaac tttattcaat ccccgaatca tttggcgcag aagttcaaat
     5941 tctgaaactt cggcgagaaa ataattacct tccggacctg gctgaattga aagagatgat
     6001 tcgtcccgat accaaactga tttgcttgaa caatccaaac aatcccaccg gtgcgttgat
     6061 tccgaatgaa atgttgcggg aggtggttga aatggctgaa aaggtcgggg cttatgtatt
     6121 atgtgatgag gtataccgtg gattaaatca gaaccatgag tataccgaat ctgttgcgga
     6181 tttgtatgaa cgggggatca gtgtcggcag tatgtctaaa attttctcac tgtccggctt
     6241 gcgtttagga tggatcgcaa cccataatca ggaagtcata aaagcctgtt tttctcatcg
     6301 ggattacagt ttgatcagct gcggaatcct tgacgaggcg gtggcttcgg aagctctgaa
     6361 gaacggaagc gcggttttca atcgcaattt gactcggatc cgcgaaaatc ttgcattatt
     6421 ggacagttgg atcagggaac aggctcatgt cagttatgtc aaaccgcaag ccgggacgac
     6481 tgcattgatt cattatgatt ttgacatacc gtcatacgac ttttgtaaac aattgttgga
     6541 taaaaccggt gtgtttttaa ccccgggagc ctgctttgat gaggagtatt gttttcgtat
     6601 cggctatgcc tgcgctaccc aaacgttaaa agacggtcta caaaagctga cggaatttac
     6661 cggcggatta tcggaaaaca gtgctatttc atagccgtca ttcttttttg atttgcagat
     6721 attctttgat ggaattcggg cggagcagcg tgatgatgcc tatgaaaatc aatccggcca
     6781 aaccgcaact ataaaatgcc actcgcggat tgaaacgttc ggcgaaaaac cccgcataaa
     6841 gatttcccag cggggtggtt ccgttcaaaa ataaactgta aatgctcatc acccgccccc
     6901 gatagcgatt ctcggtatta atttgcagtg tcgaatttac attagagagg gtcaggatat
     6961 aacaaaatcc gttgagaata tgaaaaatcc cggcggtaaa caggttattt acggtgccgc
     7021 aaagaaaagt gaaaattccg caaagaatcg aaaccgtata aaggatttta cggctgggtc
     7081 ccctgcggct ggctgtggca aagagaagtg cgccaaaaaa tgctccgatc cccataaagg
     7141 acatcaaata accgaaagtg gtctcatttc cgttgaggac atatttcgcg aaagccgagt
     7201 tcagcacatt aaaattcggg ataaacgtcg caacaatcag caaaatgatt aaactcaaag
     7261 ataaaactga gctgttcttt atatagatca aaccatcttt gatgttttcg atgatattct
     7321 taccgatcgg attttcaact tgttccggat gtgttttaat aaaaaacagg ctgaccaaga
     7381 caaaaagaaa actgatggaa ttaatcagaa agcaggagga tgcgccgaat ttcgtcatga
     7441 taatgccggc gatggaagga ccgatgactc tggcggcatt aaatgccatg gaattcaaag
     7501 ccaccgcatt cggcagatct tctttttgct cgacaagctc ataaatgaat gattgtctgc
     7561 agggggaatc aaaggcgttg aaaatcccgt aaatgaaaga taaaacaatc atcaacccaa
     7621 agtttaacca gtcgagataa atcataatca caaaagataa agagactaag aggaacccga
     7681 cctgggtgat gattaaaact ctttttttat tgactcggtc caaaatcgcg ccggagaata
     7741 gcgatagtaa caaggtcggg atgaactggc ttgccgaaac cagactgacg agaaacggat
     7801 tattggtggt ggttaatgcc agccacggca gtgcgatatt ctgcatccaa gtgccgatat
     7861 tggatatgca catgcctacc caatagagcc tgaaattcgg atagcccaat gaataaaaag
     7921 gattattgta tttttttatt gaattttcca tcttcctagt ctcttcttcg ttgggtcgaa
     7981 gaagatttca cgtgccgctt ataaaattca agatagaaat cggtaggctt gcacaaccgg
     8041 aaattgatgg agattgaaac tgattcaagt cgattttggg ataaaactta caagtttccc
     8101 ctaccgttag cgatgattat aaatccggaa attcttggag aataaaaaat ttttctattt
     8161 ttattattcg attttattga ggattaacca ctttttcgat gatatgacga gagaagttcg
     8221 gcgattgaac cgagtctgat ttatggttcg catatccgaa caaacagggt tcggatactt
     8281 gaggttatc
//
LOCUS       DAOSMM010000075         8039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_8947_length_8039_cov_9.228582, whole genome shotgun sequence.
ACCESSION   DAOSMM010000075 DAOSMM010000000
VERSION     DAOSMM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8947_length_8039_cov_9.228582"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK898_08995"
     CDS             complement(<1..414)
                     /locus_tag="PK898_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOU20207.1"
                     /translation="MSEVKNILLCGVGGQGTILASKILSTALVAAGYDVKMSEIHGMS
                     QRGGSVVTQVRYGDKVYSPIIGKGSADILVSFEAMEALRYLDGLKKDGVAVVNDYRIP
                     TATTLSGQETYPADCIEQLKTLVNTKVLDAAKIATE"
     gene            complement(407..2179)
                     /gene="iorA"
                     /locus_tag="PK898_09000"
     CDS             complement(407..2179)
                     /gene="iorA"
                     /locus_tag="PK898_09000"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOU20208.1"
                     /translation="MKQLLTGNEAVARGAWEAGVRYASAYPGTPSTEILENIAQYREI
                     YSEWAPNEKVATESAIGASFAGGRALAAMKHVGLNVAADPLFTGAYIGVNGGLVIVSA
                     DDPGLHSSQDEQDNRYYARAAKVGMLEPSDSQEAKDFVGIALELSEQFDMPILLRMTT
                     RVCHSKSIVDLSKERTEKPPVPYAKQDKFNPIPAISKRLHTKVEQNLKRLEEYSESSP
                     LNYVEWGEKKIGIISSGVAYEYAKEVFGENASYLKLGFTFPLPMRKIREFAARVDQLI
                     VVEELEPIMENEIKAAGIPCIGKEKIPCEGELNPDILRQSLLGEEHETIRYDASLVAA
                     RPPVLCAGCPHRGFFYELQKMKNVITVSDIGCYGLAPKDIAICMGGGFSVAHGAQKIF
                     DSESDGTRKRCVGIMGDSTFFHSGMTSMLEAVYNRSDILMVVLDNRITAMTGHQQNPG
                     TGLTLAEEPTHMTDIAEVAQALGVKHIRTVNPLKPDDVRDALKWGMGFDEPALLITRW
                     PCALKKMTQTDIEEFGSLRFVNCVDPEKCIGCRACIQTGCPAIEYHADSKKVTIDQIQ
                     CTGCDLCAQVCPKHAIFREEKQNV"
     gene            2389..3039
                     /locus_tag="PK898_09005"
     CDS             2389..3039
                     /locus_tag="PK898_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU20209.1"
                     /translation="MIKKNSLVDQVYEELRNGIISLRIPLGSRLNVNELQDTLQVSCT
                     PIREAINRLQQEGLVTYENNVGARVLNLDEHDVEEIQQLAMTLHREAIRLAMEREDHA
                     ILLSELEQHLSEYVDAETVQDEVMAVNHLVGTFYHHCGNRRLDKSMISIQGQQLLLRY
                     IFATYRKDRKSGSDFAPLIQAVRESDVEAACRALQQNADGNTPVILDYLRKRNANG"
     gene            complement(3098..4474)
                     /locus_tag="PK898_09010"
     CDS             complement(3098..4474)
                     /locus_tag="PK898_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20210.1"
                     /translation="MAIPFYAKSTGNEGFIKNMEVVGFSNLNGVNAFQMALYKTADGK
                     YYMYCGSFRGPGWNIVDVTDPAHPTVVNWLEACDPKEFPATNTPKIQVADGLMIGALG
                     GGVPFLHGNKPGDKNKSGLMIFDIRQDPIHPKLLSFWDTGVKDGMGVHRFMYNGGRYV
                     HLSADCPGFTGNIYRILDIIDPTHPTEVGRWWDPRQFTDGLREGEYPVDGAQHWEFMD
                     WPQLHGPPFAVGNLAYLSYYSEGLIILDISDITRPKKIGQLKLKGPFSGKYAGARTHT
                     CLPLPGRSFLVATNEGERFPFYNKKILSEGPGKGAQPMNNLHMIDISDPSDPQLIAEF
                     PYPEVPENFPYRNFNTAGLDGAQGPFGPHNIHEPMSGKPWLDQNADRVYCCYFHAGMR
                     VYDVSDPYYIKELAYFIPPNPEKLLYEIPVPGPMLATTEDCVVDDRGFIYLDTWQDGM
                     YIVRVKNT"
     gene            complement(4508..4984)
                     /locus_tag="PK898_09015"
     CDS             complement(4508..4984)
                     /locus_tag="PK898_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20211.1"
                     /translation="MFRKDQFGLVFNTIFCLMFAIFIPLFMEWQNGVLGLEAFCKDFI
                     PGFTVALTVATYIDLKAMGDSFARKCGVKNENGLLFHILRVASITFVMTAIMTVVMTF
                     LAVGFSLGPVFFLVCLKAFPAVYAVALVVAFITFAFGMKLTAALCVKPPKSMPAFN"
     gene            complement(5075..5914)
                     /locus_tag="PK898_09020"
     CDS             complement(5075..5914)
                     /locus_tag="PK898_09020"
                     /inference="COORDINATES: protein motif:HMM:NF037296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20212.1"
                     /translation="MAIEFRPMRSIIYVDCCREEYRHRLQHWLYGHHIEDSISVFGPY
                     VTKYAFYNALPVPPEGERFGTRRMQMTEHYWLVNEMVPEMRNKAVSEYMPLNVLRWQG
                     CVPDTDETPDMNIDGDAGRSAGGDNGCQPFVFAHVPISWEEDFKGKGRMVIDGPNYRW
                     QFVIQYPSDLGFEEGENWFHNEFVPFFQSCPQVNRFLSSRIMQDVVGCPFHRLVEMWF
                     DGPEEWYQAAVTGTKDWKKPFWAQQDQFPFFKSQFNIVGMFLTDIPASDNLTQYRGYI
                     TMR"
     gene            complement(5934..6869)
                     /locus_tag="PK898_09025"
     CDS             complement(5934..6869)
                     /locus_tag="PK898_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOU20213.1"
                     /translation="MKILGISGGTPNGNNDAMCKQALMGAQEVGAEIEFINLNKLDIK
                     HCTGCKSCVMALFSGKGSLCSLKDDFNWLLDKIYDADGIVWSIPIFEKGSTGLFHTIT
                     DRFGPRMDRGNVIIGNKISSENGGVPIDPRFMEDKVVSYMGVGGSDWNTLIQCDFGMQ
                     ALTPMWKIIDNEIFPWCLDIIMNDAALERATQIGRNIAEAAKDYSKAEYKGIDGICPH
                     CHSRNFFLDEDGKAICCLCGIEGKIEVVDGKYKFTFPEEQLKHAHDTLSGKFLHGDDI
                     QRNEAKARAAMQTEEAKARQKKYREFITPLVPERK"
     gene            complement(7063..7965)
                     /locus_tag="PK898_09030"
     CDS             complement(7063..7965)
                     /locus_tag="PK898_09030"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOU20214.1"
                     /translation="MTFFQLECFMVLAQRLNFTQAAAELFIAQPALSRIISSLERELK
                     VQLFERTPRSVTLTPAGKAFLSRCPTVLESYRNCVSAAHLAQKGLNGSLTLGILRDNF
                     EPCLPQLFKTFHNHFPQIALSLRGYSHSGLLAAFEFGEVDAILNYLQIPAAAENEELI
                     VLHRNRQCVITALDHPLAQRSSLRMAELKDEPFVVMTRTDSTPGHDFIWRIAAEAGFT
                     PQIVEEATHIPILITLVACGVGISTLTEDLKYLAQGKVAFIPLLGVPLSNLVFTWHSN
                     HTNPALPHLIQTVRSQAEGESCGS"
BASE COUNT         2087 a         1957 c         1871 g         2124 t
ORIGIN      
        1 ttcggttgcg atctttgcgg cgtcgagcac tttggtgttt accagagtct tcagttgttc
       61 gatgcaatct gccggatagg tttcctgtcc cgaaagggtg gtggctgtcg gtatccgata
      121 atcattgacc actgccactc cgtctttttt taatccgtct aaataacgca gggcttccat
      181 cgcctcaaag gagaccaaaa tatccgcact tccttttccg atgatgggag aataaacctt
      241 atctccgtaa cgtacctgag tgacgacact gccgccgcgt tgactcattc catgaatttc
      301 actcatttta acgtcataac cggccgctac caaggctgtc gaaaggatct tgctggctaa
      361 aatggtacct tgaccgccga caccgcaaag taaaatattt tttacttcag acattttgct
      421 tttcctccct gaaaatggcg tgtttcggac aaacctgcgc acataaatca cagcctgtgc
      481 attgaatttg atcgatggtg actttctttg aatccgcatg gtattcgatt gccgggcagc
      541 ctgtttgaat acatgcccga catccgatgc atttttccgg atccacacag ttgacgaaac
      601 ggaggctgcc gaattcctca atgtccgttt gagtcatctt cttcagcgca cagggccacc
      661 ttgtaataag cagagcgggt tcatcaaatc ccattcccca tttcagagca tcccgaacgt
      721 catccggctt caacgggttg acggtccgaa tatgtttgac ccccaacgct tgcgcgacct
      781 ccgcgatatc ggtcatatgg gtgggttctt ctgctaaggt caagccggtt cccggatttt
      841 gttggtgacc ggtcatggcc gtgatgcgat tgtctaaaac aaccatcaga atgtcggagc
      901 gattgtaaac cgcctccagc atggaagtca tcccggaatg aaaaaatgtg gaatctccca
      961 ttatccccac acaacgtttt cgcgtgccgt cagattccga atcgaatatt ttttgagcgc
     1021 catgcgcgac ggagaaaccg ccgcccatac aaatggcaat atccttgggt gccaaaccgt
     1081 aacaaccgat atcgctgacc gtaatcacat ttttcatttt ttgcagttca tagaaaaagc
     1141 cgcgatgcgg gcaaccggca cagagaacgg gcgggcgggc ggcaaccagc gaagcatcat
     1201 agcggattgt ttcgtgttct tcacccaaca atgattgccg caggatatcc ggatttaatt
     1261 caccctcaca cggaattttt tctttcccga tgcaggggat gccggccgct ttgatctcgt
     1321 tttccatgat gggttccaac tcttcgacta caatcaattg atcgacccgg gcggcaaact
     1381 cacggatttt tcgcatcggc agcggaaatg taaaaccgag cttcagataa gaggcatttt
     1441 cgccgaaaac ctctttggca tattcataag cgacgccgga ggagataatg cctatttttt
     1501 tctcgcccca ttcgacatag tttaatgggg aactctcgga atattcttcc aaccgtttca
     1561 gattctgttc cactttggta tgaagccgtt tggaaattgc cggaatggga ttgaatttat
     1621 cctgcttggc ataaggtacc ggcggctttt ccgtccgttc tttactcaaa tctacaatac
     1681 ttttggaatg gcacactcga gttgtcattc gtaaaagaat cggcatgtca aattgttcag
     1741 ataattccaa cgcgattccc acaaaatctt tcgcttcctg cgaatcggag ggttcaagca
     1801 ttcccacttt ggcggcgcgg gcatagtatc ggttgtcttg ttcgtcttgc gaggaatgca
     1861 accccggatc gtccgctgag acaatcacca atcctccgtt cacgccgata taagcaccgg
     1921 tgaataacgg atcagccgca acgttcaacc cgacgtgttt cattgccgcc aaagctcgtc
     1981 cgccggcgaa tgaagctccg atggcggatt ccgtcgccac tttttcattt ggtgcccatt
     2041 cggaataaat ttcccgatat tgcgcgatat tttccagaat ttccgtactg ggtgtgcccg
     2101 gataagcgga ggcataacgg actcccgctt cccatgcccc tctggcaaca gcttcgtttc
     2161 ctgtcagtaa ttgcttcaaa gttgttcctc ctttgtaaaa tatacgatat atgttatatc
     2221 ttgcaacatt tatatgaatt agaatatcac agattgatac cttgtcaaga tgtgtgttgt
     2281 aacctttttg cagatcattt tccgaattcc ttgcgatttt aaaacggaat tggtatgata
     2341 aatttatatt ttgcaagaaa caaaatttga agggagccaa tcgatcctgt gataaaaaag
     2401 aattccctgg tggaccaggt ttacgaagaa ttgcgaaacg gaattatttc gcttcgaatc
     2461 cctttgggga gccggttgaa cgtcaatgag ctgcaggaca ctttgcaggt cagttgcaca
     2521 ccaatccggg aggcgatcaa tcgtctgcaa caggaagggt tggtcactta tgaaaataat
     2581 gtcggtgcgc gtgtgctcaa cttggatgaa catgatgtcg aagaaatcca acaattggcg
     2641 atgacattgc accgtgaggc gatccgtctc gcaatggaac gggaggatca tgcgatattg
     2701 ttgtccgagt tggaacagca tttatcggaa tatgttgatg cggaaacggt tcaggatgag
     2761 gtaatggctg tgaatcatct ggtgggaacg ttttaccatc attgcggaaa tcgccgtttg
     2821 gacaagagta tgatttccat tcaaggccag caactcctgt taagatatat ctttgcaact
     2881 taccgtaagg atcggaaaag cgggtcggat tttgccccgc tgattcaggc tgttcgtgag
     2941 tcggatgtcg aagcggcctg ccgggcgtta caacaaaatg ccgacggtaa caccccggtg
     3001 attctcgact atttgcggaa gaggaatgca aacggttaga ccgatgttct aaaagcccca
     3061 tgagcttacg aatcatgggg ctttttttgt atagcgatca ggtgtttttc actcggacaa
     3121 tgtacatacc gtcctgccag gtgtccagat aaatgaagcc gcgatcatcg accacacaat
     3181 cctcggtcgt cgccaacatc ggaccgggaa ccggaatctc atagagcagc ttttcgggat
     3241 tgggcggaat gaaataggcc agttctttga tatagtacgg atcggacaca tcatacacac
     3301 gcatccccgc atgaaaatag cagcaataaa cgcgatcggc gttttgatcc aaccatggct
     3361 ttccgctcat cggttcgtga atattatgcg ggccgaaggg tccctgagct ccgtccaatc
     3421 ctgcggtatt gaaatttcga taagggaagt tttccgggac ttccgggtag gggaattccg
     3481 caatcaattg cggatcggac ggatcggaaa tgtcaatcat atgaaggttg ttcatcggtt
     3541 gcgcaccttt gccgggaccc tcggacagga ttttcttatt gtaaaagggg aaacgttccc
     3601 cttcattggt tgccaccaag aagcttcttc ccggcagagg cagacaggtg tgagtgcgcg
     3661 ctccggcata tttcccggaa aaaggtccct tcagctttaa ttgaccgatt ttctttggac
     3721 gggtgatgtc ggaaatatcc aaaatgatca gcccttcact gtaataggag agataagcca
     3781 gatttccgac cgcgaagggt ggtccgtgca gctgcggcca atccatgaat tcccaatgtt
     3841 gagcgccgtc gaccggatat tcgccttctc tgagtccgtc ggtgaattgt cggggatccc
     3901 accaacgacc cacttccgtc ggatgagtgg ggtcgatgat atcaagaatc cgatagatat
     3961 ttccggtgaa acccgggcaa tccgccgata aatggacata tcgtccgccg ttatacataa
     4021 aacggtgaac tcccattccg tcttttacgc cggtgtccca aaaagaaaga agtttgggat
     4081 gaatgggatc ctgccggatg tcgaaaatca tcagaccgct tttgttcttg tctcccggct
     4141 tattgccgtg aagaaacgga acaccgccgc ctaacgctcc gatcatcaat ccgtccgcca
     4201 cctgaatttt cggcgtattg gtagcgggga attctttcgg gtcgcaggct tccagccagt
     4261 taaccaccgt cggatgagcc ggatcggtta cgtcgacaat attccagccg ggcccgcgga
     4321 aagatccgca atacatgtag tatttgccgt ccgctgtttt atataatgcc atctgaaaag
     4381 cgtttactcc gttcagatta ctgaatccga caacctccat gtttttgata aaaccttcgt
     4441 tgccggtgct tttcgcataa aacggtatcg ccattaaaaa acctcttctg ctttcgagtc
     4501 tgaaaaatca attaaatgcg ggcatgcttt tgggaggctt tacacataat gctgctgtca
     4561 gcttcatacc gaatgcgaaa gtgatgaaag ccactaccaa agctactgca taaacagccg
     4621 ggaaagcttt cagacagaca aggaagaaaa cgggtcccaa agagaatccg accgctaaaa
     4681 atgtcatcac aacggtcata atcgctgtca tgacaaatgt gatggacgcg acccgtaaaa
     4741 tatggaaaag gagaccgttc tcatttttaa cgccgcactt tcgggcaaaa ctgtccccca
     4801 tcgctttcaa atcgatatag gttgcgacag tcaatgccac ggtgaagccg ggaataaaat
     4861 ctttacaaaa tgcttccaaa cctaaaacgc cgttctgcca ttccataaac aacgggataa
     4921 aaatggcgaa catcagacaa aagatggtgt tgaacaccaa accgaattga tcttttcgaa
     4981 acatattttt ctccggataa aagaatcatt tcctgttctc tcggatgaaa agtctcatat
     5041 taaaaaacga ttgatcagag aacaggaatc tgtttcaacg catggtaata taccctcgat
     5101 actgtgtcag attgtcactg gcggggatat cggtcaggaa cataccgaca atattgaatt
     5161 gtgacttgaa gaacggaaat tgatcttgtt gagcccaaaa cggctttttc caatctttgg
     5221 tgccggtcac ggcagcttga taccattctt ccggtccgtc aaaccacatt tccaccaacc
     5281 ggtggaaggg acaacccacg acatcctgca taatacggct ggagaggaaa cgattgactt
     5341 gcgggcagct ttggaaaaat gggacgaatt cattatggaa ccaattttcg ccttcttcaa
     5401 aaccgagatc gctcgggtac tgaataacga attgccagcg ataattcggt ccgtcaatga
     5461 ccatgcggcc ttttccttta aagtcttctt cccatgaaat gggaacatgc gcaaagacaa
     5521 aaggctgaca accgttgtct cctccggctg atcgaccggc gtctccgtcg atattcatat
     5581 cgggagtttc atccgtgtcc ggaacacatc cctgccaccg taatacattc aacggcatgt
     5641 attcggaaac ggctttgttg cgcatttccg gcaccatttc attaaccagc caatagtgct
     5701 cggtcatttg catccggcgt gttccgaatc gctctccctc gggagggacg ggtaaggcgt
     5761 tataaaaagc gtattttgtt acataaggac caaagactga gatgctgtct tcaatatgat
     5821 gaccatacag ccagtgctgc aatcgatgac gatattcttc tcggcagcaa tccacataaa
     5881 tgatgctgcg catcggtcga aattctatag ccatggtaaa aaaaccttct ttcttatttt
     5941 cgctcgggaa ccaacggggt gatgaattcc cgatatttct tttgtcgggc tttcgcttcc
     6001 tcggtttgca tcgcggcacg ggctttcgct tcattccgct gaatgtcgtc cccgtgaagg
     6061 aatttgccgg ataaagtatc atgggcatgc ttcaactgtt cttcgggaaa agtaaatttg
     6121 tattttccgt caaccacttc gatcttacct tcaatcccac aaaggcagca gatcgctttg
     6181 ccgtcttcat ccaggaaaaa gtttcgactg tggcaatgag ggcaaattcc gtcgattcct
     6241 ttatattctg ctttgctgta atctttcgca gcttcggcga tatttcttcc gatttgtgta
     6301 gcccgctcca gagcggcatc attcataatg atatccaagc accacgggaa aatctcatta
     6361 tcaatgatct tccacatcgg agtcagagct tgcatcccga aatcacattg gatcaaagtg
     6421 ttccaatcgc tgccgcccac tcccatgtac gagaccactt tatcttccat aaaacgcgga
     6481 tcgatcggaa ctccgccatt ttcggaggaa atcttatttc cgatgatcac atttccgcga
     6541 tccatccgcg gtccgaaacg atcggtaatc gtatgaaaca gtccggttga gcctttttca
     6601 aaaataggga tcgaccagac aattccgtcg gcgtcataaa tcttatccag cagccaattg
     6661 aaatcatctt ttaagctgca aaggcttcct ttaccggaaa ataaagccat gacgcaagac
     6721 ttacaaccgg tacagtgctt aatgtccaac ttgttcagat tgatgaactc gatttcggct
     6781 ccgacttctt gcgctcccat taaagcttgt ttgcacatcg catcattgtt gccgttcgga
     6841 gttcctccag aaatacccaa tattttcata gtactccctc cgggataaag atattatttg
     6901 gtttgcaacg gattattgat tttgcttatc caaatatatc ttattctgtt acgatgcttc
     6961 tgtaaaatac cggaagtgaa tcacggctat acacttttgt tatgatgaaa aaaggaatcg
     7021 acttgataaa aggatcgaca agccgctggg ataggaaatc atttaagatc cgcaggattc
     7081 gccttcagcc tgtgatcgaa cggtctgtat cagatggggc aatgccgggt tggtatgatt
     7141 gctgtgccac gtaaagacca agttggaaag gggtactccc aaaagcggaa tgaaagcgac
     7201 cttcccctgt gccagatatt tcaggtcttc tgttagggtg gagataccga caccgcaggc
     7261 aaccagcgta atcaaaatcg gtatatgcgt cgcttcttcg acaatttgtg gagtgaaacc
     7321 ggcttccgcc gcaattcgcc agataaaatc atggccggga gtggaatcgg tacgcgtcat
     7381 aacgacaaac ggttcgtctt tcaattccgc catccgtaaa ctgctgcgtt gtgccaacgg
     7441 atgatcgaga gcggtgatga cgcactggcg attccggtga agaacaatca actcttcgtt
     7501 ttctgctgcg gcagggattt gaagataatt caggatggcg tccacttccc cgaattcaaa
     7561 agcagccagc aaaccggaat gactgtagcc gcgcagcgac agggcaatct gcggaaaatg
     7621 attgtggaag gttttaaaaa gctgtggcaa acaaggttcg aaattatcgc gcaagatccc
     7681 gagtgtaagc gagccgttta accctttttg cgccaagtgc gccgcggaaa cacagtttcg
     7741 atagctttcc aaaaccgtgg gacaccggct gagaaatgct ttgcctgctg gcgtcagggt
     7801 aacgcttcga ggtgtccgct caaaaagctg cactttcaat tctctttcaa gcgaagaaat
     7861 aatacgactg agggccggtt gggcgatgaa taattcggcg gcggcttggg taaaattcag
     7921 acgctgcgcg agcaccatga agcattccaa ttgaaaaaac gtcatgagga tcctcttaaa
     7981 atcgttatta aagaattata gcaaatccct ttaatcgatg tcgtttttcg gcggatcgg
//
LOCUS       DAOSMM010000076         7964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_9064_length_7964_cov_16.835630, whole genome shotgun sequence.
ACCESSION   DAOSMM010000076 DAOSMM010000000
VERSION     DAOSMM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9064_length_7964_cov_16.835630"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..624
                     /gene="dcd"
                     /locus_tag="PK898_09035"
     CDS             52..624
                     /gene="dcd"
                     /locus_tag="PK898_09035"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOU20215.1"
                     /translation="MGVLCDHAIKELIDNGLIDFNRSEITYESVITQVNPNTLDLSLG
                     RFVRWPKEHSGQVIFGKAHHSDDFWELQEIDPKEGILLKPGDIFLGSSREFLRMPGDI
                     CGQVYTKSSLGRLFINHMMAGVIDAGFQGTITLELKNEGKHNVIIPYGARVVQIQFSR
                     LESKPDRDYSQRQNRYQGQIMPETAREEKN"
     gene            complement(621..1580)
                     /locus_tag="PK898_09040"
     CDS             complement(621..1580)
                     /locus_tag="PK898_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU20216.1"
                     /translation="MLERGSAQPILFIQSIKEAALHTIDLHMHSKYSNDGEFEPHTLM
                     DLCSKVPLDLVSLTDHNSAKGNVEAAKRAKELNMLFIPGIEINCQTKKRTEIHMLGYG
                     IDPTDSAFEKIEKEVIEREYEADLLRIRIFRKLGVILDEEKIQKETEKQPLTYRVIAE
                     VALSEYRNRDHPLLQPYLPGGNRSDNPLVNFFWDLCTTGKPADVPVDYLPVPKAIQLI
                     QESGGIAVFAHPGASIGKNDAELAEIVEAGISGIEVYSSYHDREMTRFYEIQAQKYGL
                     RVSVGSDFHGSLKPAIQLGSVSCNGREEEIYSFMQESISSSPA"
     gene            complement(1580..3367)
                     /gene="thrS"
                     /locus_tag="PK898_09045"
     CDS             complement(1580..3367)
                     /gene="thrS"
                     /locus_tag="PK898_09045"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOU20217.1"
                     /translation="MAAKHNAEYENSELYKIRHSTAHIMAEAVLEKFPEGKVAIGPAI
                     ADGFYYDFDLPRALTPEDLEEIEHRMKKIIKGNHPFIKEVISAEEAKKIFADQPYKIE
                     LIEGLEKGGMDEDGNPTDEKPEISIYKQDKFVDLCRGPHVSNTSEINPNAIKLLNVSG
                     AYWRGDEKRPMLQRIYGTAWETEAELKDYLWKQEEAKKRDHRKLGKELDLFSVSDEVG
                     QGLILWHPKGAKVRKLIENYWSEEHEKWGYDFVYTPHIGKSQLWETSGHLGFYSENMY
                     SPIDIEGTKYYIKPMNCPFHLYIYKSNLRSYRDLPIRYAEMGTVYRYERSGVLHGLTR
                     VRGFTQDDAHHFCRPDQMPAEIDFVVEFSLHILRAFGFEEFQAYLSTKPEKSVGEPER
                     WKAAESALEDSLKRHNLPYEIDPGGGAFYGPKIDFKVKDAIGREWQLTTIQFDFNEPE
                     RFDLTYIAEDGQPTRPYMIHRALLGSMERFFGVLIEHYGGAFPAWLAPVQAEIIPIAD
                     RHIDYANELAAQLRQNRFRVHVDTRTDRMNAKIRDAQKEKIPYMLIVGDKEMENQQVS
                     LRKRSGEDLGAMPVADFISLATDEVEKKI"
     gene            3734..4792
                     /locus_tag="PK898_09050"
     CDS             3734..4792
                     /locus_tag="PK898_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU20218.1"
                     /translation="MKSANNIGFGIIGLGMIAEFHAKAIENIEGCKFVAGYDHTPAHS
                     ESFCALHGGTPYTDLEKFLADPDLDIVTIATPSGFHLEGAVRAANAGKHIIVEKPLEI
                     STERCDKIIEAAVKNNVRMCTVFPSRFHDSARLIKEAVRQGRLGKIVLADAQIKWYRS
                     QEYYDSGGWRGTWKFDGGGVLMNQGIHAIDLLQWVMGDVSEVTAYTATLAHERIEVED
                     TAVAILKFSDGALGMIEGTTAAYPGFLKKLEICGSKGSVMMEEESITKWEFSEPMPED
                     QKIRERFQNNTQTGGGAGDPKAISFEGHRKLFSAFVDAVIRNKPFDLEGNEGRKSVEI
                     IENIYKSAKNGVSVKFRS"
     gene            complement(4946..6871)
                     /gene="gyrB"
                     /locus_tag="PK898_09055"
     CDS             complement(4946..6871)
                     /gene="gyrB"
                     /locus_tag="PK898_09055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOU20219.1"
                     /translation="MTQNKNGNSQYDASNIQVLDGLEAVRKRPGMYVGGTDSKALHHL
                     VYEVVDNAIDEALAGFCDQIDVIIHPDNSVTVRDNGRGIPTDIHPQMKISALEVVLTT
                     LHAGGKFGGGSYKVSGGLHGVGSSAVNALSEWMEAKVWRDGKIYFQRYNRGKPEERVK
                     VIGECPEDQTGTEISFLFDKEIFKEEPGYRYETLVNRFREMAFVTKRTKIHLVDEKDN
                     KETTFYFQGGISSFVHYLNKDNVPLHPIVHAEKTIENVGVEVALQYTDVYTETVYSFA
                     NTINTIDGGTHLTGLRTAITRVINDYARSANLLKESDPNFSGDDTREGLTAVVSIKHP
                     DPQFESQTKVKLMNTEVSTYVQQVIGECFKDFLENNPAAAKAIVNKCLTSSRARDAAK
                     KARDLVIRKSALESMTLPGKLADCSGRNPEQTELYIVEGDSAGGSAKQGRDRGFQAIL
                     PLRGKILNTERARLDKILSNNEVKAMISAIGCGIGENFDIKGLRYGRIIIMTDADVDG
                     SHIRTLLLTFFFRYMPELVEQGHLFIAQPPLYRTTYKGVHRYAYSDAEQAAILKELGS
                     GSEKASIQRYKGLGEMNAEQLWETTMNPAYRTLLKVTVDDASQAAKTFEMLMGDEVPP
                     RTRFIQTHAHEVRNLDI"
     gene            complement(6868..7863)
                     /locus_tag="PK898_09060"
     CDS             complement(6868..7863)
                     /locus_tag="PK898_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20220.1"
                     /translation="MEQKRRVEKIALPSITKALGSGFNLIAENIHFIVLPILFDLLVW
                     LGPRFRAYDLLKGNVISLSQQIDQNAPANLLNQMNSLRDIALSALEQTNIMGAIHFFP
                     ISVPILLGGNASMAGPIDNLQIIELHSVFVLFLLIALLALTGLLFGCFYYSAIAKKTS
                     IIAYRLTMHRFFRQLLNSFVAVLLLFISLVILLIPISCILSILLMISSALAQFLSMIF
                     MIMIAWLIIPLFFTMHGIFLGNGVIESLKMSFNLARWFSTSTSFFIVAIVVISQGMNL
                     IWTIPQPDSWLLLIGITGHAFITTSLISASFILYQQYLSWISENEELITKGIIQK"
BASE COUNT         2177 a         1879 c         1681 g         2227 t
ORIGIN      
        1 gacaaggctg ccatgaattt caatcttgcg gctatttcga aaagaggata aatgggcgta
       61 ctctgtgacc atgcgatcaa agaactgatc gataacggat taatcgattt taatcgttca
      121 gaaataactt acgaatcggt tattactcag gtcaatccca atactttaga tctttcattg
      181 ggacgttttg ttcgctggcc gaaagaacac tcgggacagg taattttcgg aaaagcccat
      241 cactcggatg atttctggga attgcaggaa attgatccga aggaaggaat tctgcttaaa
      301 cccggcgata tatttctcgg atcctcccga gaattccttc ggatgcccgg tgatatttgc
      361 ggtcaggtct atacgaaatc aagtttgggt agacttttta taaatcatat gatggccggc
      421 gtgattgacg ccggtttcca aggtaccatt acattggaac tgaaaaatga aggaaaacac
      481 aatgtgatta ttccctacgg cgcccgggtt gttcaaatcc aattcagccg tctggagtcc
      541 aaacccgaca gagattattc ccaacgtcag aatcgctatc aagggcagat tatgcccgaa
      601 accgcgcgag aggaaaaaaa ctaagcaggc gatgaagaga tcgattcttg cataaaagaa
      661 tagatctctt cctctcttcc gttacaggat acacttccca attgaatcgc cggcttcaag
      721 cttccatgga aatcacttcc gacactgacc cgcaagccgt atttctgcgc ctgaatctca
      781 taaaatcgag tcatttcccg atcatgataa ctgctgtata cttcaatccc gctaatgccc
      841 gcttccacaa tttcggctaa ttcggcgtcg tttttcccga tactcgcacc gggatgagca
      901 aataccgcta taccgccgct ttcttgaata agctggatcg ccttcggaac gggtaaataa
      961 tcgacgggta catcggcggg ttttcctgtt gtacataaat cccaaaagaa attgaccaag
     1021 ggattatcgc tgcgatttcc gcccggcaga taaggctgca acaagggatg atcgcgattt
     1081 ctgtactcgc tcaaagcgac ttcggcgatc acccgatagg tcaatggttg tttctcggtt
     1141 tctttttgaa tcttctcttc gtctaagatg actccgagtt tacgaaagat tctgattcga
     1201 agtaaatccg cttcatattc tctttcaatg acctcttttt cgattttttc gaaagcacta
     1261 tccgtaggat cgatcccgta tccaagcata tgaatttcgg ttcgtttttt tgtttgacag
     1321 ttaatctcaa ttccgggaat aaaaagcata tttaattctt tggctctttt tgccgcttcg
     1381 acatttcctt ttgccgaatt atgatcggtc agagaaacca aatccagcgg tacctttgag
     1441 caaagatcca tcaacgtatg cggctcaaat tctccgtcgt tactgtattt tgaatgcata
     1501 tgtaaatcga tcgtatgcaa agctgcctct tttatggatt gaataaagag gatcggctgc
     1561 gccgatcctc tttcgagcat cagattttct tttcgacttc atcagtcgcc aatgagataa
     1621 aatcggcgac aggcatcgct cccaagtctt ctccgctgcg ttttcgtagg gacacctgtt
     1681 gattttccat ttccttatcg ccgacgatca gcatatacgg tattttttct ttctgcgcat
     1741 cgcgaatttt tgcgttcatt cgatccgtcc gggtgtccac atgaacccgg aagcggtttt
     1801 gccgcagctg cgccgccaat tcattggcat aatcgatatg gcgatcggca atcggaatga
     1861 tttcggcttg aaccggagcc agccacgccg ggaaagctcc gccgtaatgt tcaatcagaa
     1921 cgccgaaaaa gcgttccatg cttcccaaca gcgcccgatg aatcatgtaa ggtcgagtcg
     1981 gttgaccgtc ttcagcgata taggttaaat caaatcgctc cggctcattg aagtcaaatt
     2041 ggatagtagt caactgccat tctcgtccga tggcatcctt caccttaaaa tcgatcttcg
     2101 gtccgtaaaa agccccaccg cccggatcga tttcataagg tagattatgt cgcttcagcg
     2161 aatcttccaa agccgattcg gctgctttcc aacgttccgg ctctcctact gatttttcag
     2221 gtttggtact gagatatgcc tgaaactctt caaaaccgaa cgcgcgcaga atgtgcaggg
     2281 aaaattccac aacaaaatcg atttccgcag gcatttggtc gggacggcag aaatgatgag
     2341 catcgtcttg cgtaaaaccg cgaacgcggg tcagtccgtg cagaacgccg cttcgttcat
     2401 aacggtaaac cgttcccatt tcggcgtatc ggatcggtaa atcgcgataa gaacgtaaat
     2461 tgctcttata aatatacaga tggaacgggc agttcatcgg cttgatatag tatttcgttc
     2521 cctcgatatc gatcggagaa tacatattct cgctatagaa accgaggtga ccggatgtct
     2581 cccagagctg ggattttccg atatgcgggg tatatacgaa atcatatccc catttttcat
     2641 gttcctcgct ccaataattt tcgatcagct ttcgaacctt agcgcctttt ggatgccaca
     2701 agattaaacc ctgccccacc tcatcactga cgctgaacaa atccagttct ttcccaagtt
     2761 ttcggtgatc gcgtttcttg gcttcttcct gtttccacag ataatctttc agctccgctt
     2821 cggtctccca tgccgtccca taaattcttt gcagcatcgg gcgtttttca tctccccgcc
     2881 aataagcacc ggaaacattc aataatttaa tcgcattggg attaatttcg gaggtattgg
     2941 aaacatgagg acctcggcaa agatcgacaa acttatcttg tttataaata gatatctcag
     3001 gtttttcatc ggtcggattc ccgtcctcat ccatcccgcc tttttcaagc ccttcaatca
     3061 actcgatttt ataaggttga tcggcaaaaa tcttctttgc ctcttccgca ctgatgactt
     3121 cctttatgaa ggggtgattg cccttgataa tttttttcat ccgatgttcg atctcttcca
     3181 aatcttcggg cgtcagcgct ctcggaagat caaaatcata gtagaaaccg tccgcaattg
     3241 ccggtccgat cgccactttt ccttcgggaa atttttcaag gacagcctcc gccataatat
     3301 gggcagtgga atgcctgatt ttatacaatt ccgaattctc gtattcagcg ttatgttttg
     3361 cagccatata tcctccgtaa taaaaataaa aaaaactcac cccataaccg gggcgagtct
     3421 gtaaagtctc gtggttccac ccagcttgat cactctcata aagcgacccg ctcagataaa
     3481 tgatatcggt atttcaccgg ctgccttaat cagttcggca gcgagctccc agatggtttt
     3541 cgtcagactc cttcggaccg attctcacct tctttccggt ctctctgtgc gactggtctg
     3601 acgcattgtt ctgttcaacg cttccggata aaattataca tgatctgtcg ctctttgtaa
     3661 aacattatga cacaatgagt taccgcagat aaaaataatt cgataaagca ttttttcact
     3721 ataaggagtc ctcatgaaat cagcaaacaa tatcggtttc ggtattatcg gtttgggcat
     3781 gatcgcagaa tttcacgcaa aagccattga aaatatcgaa ggctgcaaat tcgtcgccgg
     3841 gtatgatcat acgccggctc attccgaatc cttctgcgct ctccatggcg ggacccctta
     3901 tacggatttg gaaaaatttc tggctgatcc cgaccttgat attgtaacaa tagcgactcc
     3961 gtccggtttt caccttgaag gagctgttcg cgcagcgaat gcaggcaaac atattatcgt
     4021 tgaaaaaccc ttggaaatat ccaccgaacg ttgtgacaag attattgaag cagctgttaa
     4081 aaacaatgtc aggatgtgta ccgtcttccc ctcccgcttt catgattccg cccgtttaat
     4141 caaggaagcc gtcaggcaag gcaggctggg aaaaatcgtg ttagccgatg cccaaattaa
     4201 atggtaccgg tcgcaagaat attatgattc ggggggttgg agaggaactt ggaaatttga
     4261 cggcggcggt gttttaatga atcaaggaat tcatgccatt gatctgcttc aatgggtgat
     4321 gggcgacgta tccgaagtta cagcttatac cgccacattg gctcatgaaa gaatcgaggt
     4381 agaagatacc gctgtcgcga tcctgaaatt ttccgatggg gctttgggaa tgattgaagg
     4441 gactacagcg gcgtatcccg gattccttaa aaaattagaa atatgcggat ccaaaggcag
     4501 tgtgatgatg gaagaagaaa gtatcaccaa atgggagttt tccgaaccga tgccggaaga
     4561 tcaaaaaatc cgcgaacgat ttcaaaacaa tacccaaacc ggcggaggag ccggtgatcc
     4621 gaaagcgatc agtttcgaag ggcatcgcaa gcttttcagc gcctttgtgg acgctgttat
     4681 ccggaataag cctttcgatc tggaaggaaa tgaaggtcga aaatcggtag aaatcattga
     4741 aaatatctac aaaagtgcaa aaaacggtgt ttcggtaaaa tttcgatcat aaagttgaaa
     4801 cccagtcatg aaatcggggg aagaaaaaag ttaaagacac aaaacaatca taaaacactc
     4861 ccgaacttcg gttttgattt ccgaattccg ggagtgtcat tttctgatca aaaacgatct
     4921 gtttttagcc tcgtttacct gaatttcaaa tatccaaatt cctgacttca tgcgcatggg
     4981 tctgaataaa ccgtgttctt ggcggaactt catcacccat cagcatttcg aaggtcttgg
     5041 ctgcctggga agcatcatca acggtgacct ttagaagcgt cctgtatgcc gggttcatgg
     5101 ttgtttccca taactgctcc gcattcattt ctcccaaccc tttatagcgt tgaatggaag
     5161 ctttttctga tccgctgccg agttccttca gaattgccgc ttgctctgca tcggagtaag
     5221 catatcgatg tactccttta taggtcgttc gatacaaagg cggttgggca ataaataaat
     5281 gtccttgctc gaccaattcc ggcatatagc ggaaaaagaa ggtaagcagt aatgttcgaa
     5341 tatggcttcc gtctacatct gcatcggtca taataatgat ccggccgtat cgcaaccctt
     5401 tgatgtcaaa attctcaccg atcccgcaac cgatggccga aatcattgct ttcacttcat
     5461 tattcgataa aatcttatcc agcctggcgc gttcggtatt taatattttt ccacggaggg
     5521 gcaaaatcgc ctgaaaaccg cgatcacgtc cctgctttgc cgatccgccg gcggaatcac
     5581 cttccacaat ataaagttcc gtctgttcgg gatttcttcc ggaacagtca gccaattttc
     5641 cgggaagcgt catactttcc agggctgatt ttcggattac caaatcacgg gcttttttgg
     5701 cagcatcgcg agcccgagat gaagtaagac atttattgac gatcgctttg gcagctgcgg
     5761 gattattctc caaaaaatct ttgaagcatt caccgatcac ctgctgcaca tatgtcgaaa
     5821 cttcggtgtt catcaatttc actttggtct gactttcaaa ttgcggatcg ggatgcttga
     5881 tgctgaccac cgccgtcagc ccttcccgcg tatcatcacc tgaaaaatta ggatcggatt
     5941 cctttaaaag attggcgctt cgggcataat cattgattac acgcgtaatt gccgttcgta
     6001 atccggtcag atgggttccg ccgtcaattg tattgatagt gttggcgaac gaataaaccg
     6061 tttccgtata aacatccgta tattgcaggg caacttccac tccgacgttt tcaattgttt
     6121 tttccgcatg aacaatcgga tgaagcggga cgttatcctt attcagataa tgaacaaatg
     6181 aactgattcc accctgaaaa taaaaagtgg tctctttatt gtctttttca tccaccaaat
     6241 ggattttagt ccgctttgtg acaaaagcca tctcccgaaa acggtttacc aaggtttcat
     6301 accggtatcc gggttcttct ttaaatattt ccttatcaaa caggaaactg atttccgtac
     6361 cggtctgatc ttccgggcat tcgccgatga ccttcacccg ttcttccggt tttccgcgat
     6421 tgtaacgttg aaaataaatc ttaccgtccc gccaaacttt ggcttccatc cattcggaca
     6481 atgcattgac cgccgaagaa ccgacaccat gtagcccgcc ggagacttta tatgatccgc
     6541 cgccgaattt accgcccgca tgcaacgtag tcagcacaac ttccaatgcg gaaattttca
     6601 tttgcggatg aatatccgtc gggattccgc gcccgttatc ccgaacagtt acactgttat
     6661 cgggatgaat aatcacatca atctgatcgc aaaagccggc caaggcttcg tcgatcgcgt
     6721 tatctacaac ttcatacacc aaatggtgta gagctttgga atcggttcct cccacataca
     6781 tgcccggacg ttttcgaacg gcttcaagtc cgtccaaaac ctgaatattg gatgcatcat
     6841 attgagagtt accgttttta ttttgagtca tttttgaatt attcctttag ttatcaattc
     6901 ttcgttttca gaaatccaag agaggtattg ttgatataaa atgaaactgg cgctgataag
     6961 ggatgtggta ataaacgcat gtcccgtgat tccgatgagc agcaaccatg aatccggttg
     7021 cggaattgtc caaatcagat tcattccttg agaaatgacc acgatcgcga caatgaaaaa
     7081 gcttgtcgag gtagaaaacc atctggcaag attaaaactc attttcagcg attcgatcac
     7141 accgttcccc aagaaaatgc cgtgcatcgt aaagaacagc ggaataatca gccaagcgat
     7201 catgatcata aagatcatag agagaaactg tgccaatgcg gaagaaatca tcagcagaat
     7261 cgacaatatg cacgaaatcg gaatcagcag aataaccaga gagatgaata agagtaatac
     7321 tgccacaaaa gagttcagca gctgccgaaa aaaccggtgc attgtcagac ggtaagcgat
     7381 tatggatgtc ttttttgcga ttgcagaata atagaaacaa ccgaataaca atccggtcaa
     7441 tgccaagaga gcaataagca aaaataaaac aaacaccgaa tgcaattcga tgatttgcaa
     7501 attgtcgatc ggtccggcca tcgaagcgtt tccacccaaa agaatcggaa cactgatcgg
     7561 gaaaaaatga atcgctccca tgatatttgt ctgttccaga gcggacaagg caatatcccg
     7621 caatgagttc atttgattta ataagtttgc gggtgcattt tgatcgattt gttgtgagag
     7681 agaaatcaca tttcccttca ataaatcata tgcgcgaaac cggggaccca accaaaccaa
     7741 aaggtcaaat aaaatgggta agacaataaa atggatattt tccgcaatca gattaaaacc
     7801 actgccaagc gctttggtga tgcttggcaa agctattttt tcgactcttc tcttctgttc
     7861 cattatttgt taattttatc atattcgccc ttttttccga catgatggga caaaataccg
     7921 tattaattct gcggacataa atgaaaataa ttattttatt ttag
//
LOCUS       DAOSMM010000077         7925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_9121_length_7925_cov_17.126302, whole genome shotgun sequence.
ACCESSION   DAOSMM010000077 DAOSMM010000000
VERSION     DAOSMM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9121_length_7925_cov_17.126302"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..544)
                     /locus_tag="PK898_09065"
     CDS             complement(221..544)
                     /locus_tag="PK898_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20221.1"
                     /translation="MNKKTVLLIIGLLVIAMGVLALLPGMNMGSEPKWHAIAKLVLGV
                     VAVFIALKNEKAVGITTVIVGVVLVVMGLLGLFMKVSTFSAPTWHSAAVAVVGLIVLI
                     LGARK"
     gene            676..1980
                     /locus_tag="PK898_09070"
     CDS             676..1980
                     /locus_tag="PK898_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU20222.1"
                     /translation="MKKTGPLYNIKMVSKETGLTPATLRAWERRYGIPHPVRSSGGQR
                     LYSEEDISLLKWLVARKKEGMSISRAISLWKTREIHPSVETNVSDMSSEPVRSSEENE
                     QILENLHKVWSRACLTFNEEEANFAINKALEISTPELVCTQVIQKGLAAIGKGWYTGK
                     VSVQQEHFASALATRHLNKLMESTPIPHDSDRILLICPPEEAHELVLLMLSFLLRRRG
                     WNTTFLGANTPLEKLDSVIHSTMPKLVLSAAQTLPAAASLLKLAGTVNKQSVPFAFGG
                     GIFNDIKGLSQRIPGFFIGRNLESAPRVIELLLMQHPYSSETQPLPSNYLNSLEGFKR
                     HKGNIMNRINQILSFHSFPSFQLELANTHFIPGVIAALSLGDVHFLDYLKKWLDGLLK
                     NYGLPSSLISQYYLAFQQAVQEQMDNKENPILEWLSVLKPVG"
     gene            1991..2146
                     /locus_tag="PK898_09075"
     CDS             1991..2146
                     /locus_tag="PK898_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20223.1"
                     /translation="MNDFLPDERHRKNEFESTVVFMESGESFDSLSRIDPKESGILYT
                     NRLPEAL"
     gene            2147..2887
                     /locus_tag="PK898_09080"
     CDS             2147..2887
                     /locus_tag="PK898_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU20224.1"
                     /translation="MNKKIRILLSVLGIILILLGAGLMIWNKTTAEPMPEALAFLESD
                     PDVTVETGHWLIFTPASEFPDTGFIFYPGAHVDYRAYAPAAHQIAAEGFLVIIVQMPL
                     GLAVFDTDAAKDVQAAYPQIRHWAIGGHSLGGAMAAAYAKSHPESIEGLVLWAAYPSA
                     NDSLADSEIRVVSVFGSEDGLATGKKIEASKQLLPPDTIWVKIEGGNHAQFGWYGDQA
                     GDNVATVSREKQQEQTISATIKMLEQIK"
     gene            2884..3894
                     /locus_tag="PK898_09085"
     CDS             2884..3894
                     /locus_tag="PK898_09085"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOU20225.1"
                     /translation="MIRNQWYVVLESKEVGTHPIGVVRLGEKFVFWRDQTGKVHAAAD
                     RCPHRGAMLSAGKIENGHIQCPFHGIQFDGSGKCVLVPANGKNGSIPEAMHLKATPTY
                     EAHGLIWLWWGDSKPDGIPDFFTDIDDTFYYGSAHDPWDAFYSRVIENQLDVIHLPFV
                     HRHTIGRGNRTLVDGPLVEWKNDRMIYTYVFNRVDDGTLPRKPKELTPKSATSVHLEF
                     IFPNLWQNFINEKVRVLAAFVPVDNDHTILYLRFYQKFLPVPILGKMVANLAMPVNVY
                     IAHEDRRIVITQQPKISGLKIGETLLQGDLPVIEYRRKRAALQEKNESKTVSEAPKYT
                     DR"
     gene            3970..5544
                     /locus_tag="PK898_09090"
     CDS             3970..5544
                     /locus_tag="PK898_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU20226.1"
                     /translation="MENNRKNYYDAIVIGGGLAGLTAASFLAKEGRKVLVCEQSDQVG
                     GLFNSFWRSGYLFDGGIKAIENSSVMMPMLAQLGLLEQVHFHPSPIALIIGGNIQVIR
                     QFEDVVSYFRMLQEFFPSEKAGLKKILQDTKTVYDLMDGFLSFPIPFFDLPNTGNEAR
                     AEWFRKQGALFTRIPRALSLMRQDFRPYLEKHLNNSNLINILSDLFPDGTSVFFGLGY
                     FRMFTDYYYPEGGIRTIPHLLSESLQNWGGEIRLNTRVENLLFDCGRASGVRLITGEE
                     IRADYMIAAGDLRQALTQLTPETMLPKKFNRKLKDAEVSHSVFNVFLGIDRPVESLGL
                     QGCSHVFYHPDLNGISENDRIHRPDYFRHVPQEISVPCLHQNELAPQGKTGMMISAMT
                     SWNYNGGWEREPAEYESLKEKYTSDLIAGLEKFIPQLNDHIEICFSATPLTIAQRTSN
                     TKGAIMGWSYHKERTFSRGNFLQMKSSVFTPIPGLLTAGHWTYSPGGSPVAVLTGKLA
                     AEAVLHDVNKKAEHKK"
     gene            5541..>7925
                     /locus_tag="PK898_09095"
     CDS             5541..>7925
                     /locus_tag="PK898_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU20227.1"
                     /translation="MKLYENFILRDHYDVIVVGAGLGGMAAAGLLAKRGLSVLMIEQQ
                     NKPGGACTSFKREDHIHDVGAAMLYGFGQKGFRPFHFLLNELEEPIDMISHSTLARMN
                     FEGKEIIFWPDLQRFIDELIELFPDEKEGINAFYSDLYKMYENIVIKNEVIVPPSEYS
                     PRQGLRRLINDPFSIFKMQKLLSTSTMDLLKKYFHSPELFNFYDKLCSAYAYTTAEET
                     PAVLAATMFIDNHTGGVYYPAGGAQMLPNTIEKAFERYGGQALYRQLVDEILIEDGRA
                     YGVRLQDKTEIFADRVIANATVWNIYGHLVRPRHISPDRLEWAQSLIPTFPSMTLYMV
                     VDREAIPEDSFPWEIFIENRKIIDSSDLTLYINSLVDHSLCPPDELVVMAISPNFCSW
                     PHTDDPTYRSPEYEDQKRREAERMIDQIEQHIPGFKQHIRSLIIGSPTTIERYLLKNG
                     GAVGGPKNQIGQEMLKRLHARSEWKNLYFCGDSTVMGTGAPATVVSGIGAANVILREL
                     HQPEYDTHVFPEEVVHFVQPPYHRPDYISGTPVSVENAFLAAAKCQGCLHPRCRKGCP
                     AGIDIPGFLRRMEAKNYIGAARLIHEKNPFGETCGLTCAANHLCQQFCYRRDFSGEPV
                     NIAELERWVCEQAGEDGWQKHEKSTVNGEIAIIGGGLAAMSCAYYLALSGWGISIFAP
                     ENQPGGKLSEKMRSDPVLQASFNRDIRGIFAEEIHFFGNQNPVHEADLERMLKEYACI
                     FITKDQFAKPENLYEERENFPGLFIGDEFSVNSIPPVEAVACGRKTAVEIYRYLR"
BASE COUNT         2260 a         1770 c         1769 g         2126 t
ORIGIN      
        1 ccaaatacaa gtaactcaaa ccagcccttc gctcctccca aaatcaacag gctatgcgca
       61 tttttctgaa cttgcgcttg atcgaatctc gaacgttcgt tcttttttca aaaaaagatt
      121 atgtctttga ccggcgggaa atttcatatt cataaatcaa cagagaccgc ctcaaaaaaa
      181 cagaaagcgg cctctgtaac aaaagcgaaa atcttgaaat ttacttccgt gcacccaata
      241 tcaaaacgat caaaccgaca accgctacag ccgcagaatg ccaagtcgga gcgctgaaag
      301 tactgacctt cataaataat cccaacaaac ccataacaac caataccact ccgacaatca
      361 cggtcgttat tcccaccgct ttttcatttt tcagagcgat aaatacagcg acaactccta
      421 aaacaagttt agcgatggca tgccactttg gttccgatcc catattcatg ccgggaagta
      481 atgccaaaac acccattgca atgacgagta aaccgataat caataatact gttttcttat
      541 tcatggctat ctccttttta taaattccct tgactttagt tagactttac atgtacaatt
      601 attatacatt gcggagtatt tgtcaatata ttaaaatatt taaaagatta tttttttact
      661 cacttaggaa gcaaattgaa aaaaacagga ccgctttata acataaaaat ggtcagtaaa
      721 gaaacagggt tgacacctgc gactttgcga gcctgggaac gtcgctacgg gattccgcac
      781 cctgtccgat catccggtgg acaaagattg tacagcgaag aggatatatc gcttttgaaa
      841 tggcttgtcg cccgaaaaaa agaggggatg agtatcagcc gggctataag tttatggaag
      901 acccgagaaa ttcacccttc cgttgaaaca aacgtctctg acatgtcttc ggagccggtt
      961 agatcttcag aagaaaatga acaaatttta gaaaatcttc ataaagtttg gagcagagct
     1021 tgtcttactt tcaacgaaga ggaagcgaat tttgcaataa ataaagcgtt ggagatttcc
     1081 actcccgaac ttgtttgtac ccaagtgata caaaaaggtc tggctgcaat tgggaaaggt
     1141 tggtatacgg ggaaagtaag cgtccaacaa gaacacttcg cttctgcgct tgcgaccagg
     1201 catttgaata agcttatgga atcaacccct ataccccatg attccgatcg aatactcctt
     1261 atctgccctc cggaagaagc gcatgagctt gttttactga tgctctcatt tctcctccgt
     1321 cgccgtgggt ggaacaccac ctttctggga gcaaacaccc ctttggaaaa gttggattcc
     1381 gtaattcatt caacgatgcc aaaactggta ctttccgcag cgcaaacttt gccggccgca
     1441 gcttctcttt tgaaattggc cggtacggtg aacaaacaat ctgttccgtt tgcatttggc
     1501 ggcggaattt tcaacgatat aaaaggatta tctcagcgaa tacccgggtt ttttatcggc
     1561 agaaatttgg aatccgcacc tcgggtaatt gaactcctgt tgatgcaaca tccttattca
     1621 tccgaaactc agccgttacc gagtaattat ttgaatagtt tggaaggatt taaaagacac
     1681 aaaggaaaca tcatgaatcg gataaaccaa atcctcagct ttcactcttt cccttccttt
     1741 caattggagt tggcaaacac acattttatt cccggggtaa ttgcggcgct ttcgttggga
     1801 gacgttcatt ttttggacta tttaaaaaaa tggctggacg gattacttaa aaattatggt
     1861 ttgccctctt ctttgatttc tcaatattac ttggcatttc aacaagctgt acaagaacag
     1921 atggacaata aggaaaaccc gattttggaa tggttatccg ttcttaaacc ggttggctga
     1981 gaaggaatca gtgaacgatt ttttgccaga tgaacgccat agaaaaaacg aattcgaatc
     2041 cactgtcgtt tttatggaat ccggcgaaag ttttgattcg ctctcacgca tcgatccgaa
     2101 agaaagcggt attctttaca caaatcggtt accggaggct ttataaatga ataaaaaaat
     2161 tcgtattctt ctttcagtgc tgggaatcat tttgattttg ctcggcgcag gtttgatgat
     2221 ttggaataag acgaccgcag agccgatgcc ggaagccctt gcttttctgg aatccgatcc
     2281 cgacgttacc gtcgaaaccg gtcattggct tatttttacg ccggcttctg aatttcccga
     2341 cacaggattc atcttttatc cgggtgcgca cgtggattac agggcttatg ccccggctgc
     2401 gcatcagatt gcggccgaag gatttttggt gatcatcgta caaatgccac tcggcttggc
     2461 tgtattcgat acggatgctg ccaaggatgt ccaagccgcc tatccgcaaa tccgccattg
     2521 ggcaatcggt gggcattcgt tgggcggcgc catggctgcc gcatatgcga aatctcaccc
     2581 cgaatctatc gagggcttgg tattatgggc tgcctacccg tctgcaaacg acagcttagc
     2641 ggattccgaa atccgggttg tttccgtatt cggatcggaa gacggtttgg caaccggtaa
     2701 aaaaatcgag gcatcgaaac aattactgcc accggatacc atatgggtaa aaatcgaagg
     2761 gggaaatcac gctcaattcg gctggtacgg agaccaggca ggcgataacg tcgccaccgt
     2821 ctctcgtgag aagcaacaag aacaaacaat ttcggcgacg ataaaaatgt tggagcaaat
     2881 aaaatgattc gtaatcaatg gtatgttgtt ttggaatcca aagaagtcgg cacccatccc
     2941 ataggcgtcg tcagactcgg agaaaaattc gttttttggc gcgatcagac cggaaaagtg
     3001 catgcggcag cggatcgctg cccccaccgt ggagcaatgc tcagcgccgg gaaaatcgaa
     3061 aacggacaca tccaatgccc cttccacggc atccaatttg acggttcggg caaatgtgtt
     3121 ctggtacccg caaatgggaa aaacggctca atcccggagg caatgcactt aaaagccact
     3181 ccaacctatg aagcgcatgg cttgatctgg ctctggtggg gtgattcgaa accggacgga
     3241 atccccgatt tttttaccga tatcgatgat accttctatt atggttccgc tcatgatcct
     3301 tgggatgctt tttactcaag ggtgatcgaa aatcagttgg acgttatcca cttgccattt
     3361 gttcatcgtc acacaatcgg ccgtggaaat cggactttag tcgacggtcc gctcgttgaa
     3421 tggaaaaatg acaggatgat ctatacctat gtgttcaatc gggttgacga tggcactctt
     3481 ccgcgaaaac ccaaagagct tactccaaaa tccgcgacaa gtgttcattt ggaattcatt
     3541 tttcccaatc tgtggcaaaa ttttatcaac gagaaagtcc gtgttttagc ggcattcgtc
     3601 ccggtggata atgatcatac aattctttac ctcaggtttt atcagaaatt tttaccggtt
     3661 ccgattttag gaaaaatggt agcaaatttg gcgatgccgg ttaatgttta tattgcacat
     3721 gaagaccgcc ggatcgtcat tacacaacag ccaaaaataa gcggcttgaa aataggggaa
     3781 accttgcttc aaggagattt gccggttatc gaatatcgac gaaaaagagc ggctttgcaa
     3841 gaaaaaaatg aaagcaagac cgtttctgaa gcaccaaaat acaccgaccg ataatttttt
     3901 gtccgatgaa ttcagttcat ctttttgctg ctcaagataa tattgtttta taaaatcgtt
     3961 gggatagaca tggaaaataa tcgaaagaat tactacgatg cgatcgttat cggcggagga
     4021 cttgcgggac tcacagcggc ttcattcttg gcaaaagagg ggagaaaagt gttggtttgt
     4081 gaacagtcgg atcaagtggg aggactgttc aattcatttt ggcgatcggg gtatttattt
     4141 gacggaggaa taaaagctat agaaaacagc tcggtcatga tgcccatgct ggcgcaactc
     4201 ggattgttgg aacaggttca ttttcatcca agcccgatcg ctcttattat cggcgggaat
     4261 atccaagtta ttcgacaatt tgaagatgtt gtttcctatt ttcgcatgtt gcaggaattc
     4321 tttccctcgg aaaaagccgg cttgaagaaa atccttcaag atacaaagac agtctatgat
     4381 ttgatggatg gcttcctgtc ttttccgatt cctttctttg atttaccgaa taccggcaat
     4441 gaggcacgag cagaatggtt tagaaaacaa ggtgctttat tcacgcgtat cccccgcgca
     4501 ttgtcgctga tgcgccagga ttttcgacct tatttagaaa aacatctcaa caattcaaac
     4561 cttatcaaca tactcagcga tcttttcccg gacggaacga gtgtcttctt tggattgggg
     4621 tattttcgta tgtttacgga ctattattat ccggaaggag gcatccggac tattcctcat
     4681 ctactttcag aatccttaca aaattgggga ggagaaatca ggctgaacac gcgggtggaa
     4741 aatttgcttt tcgattgcgg tagagcttcc ggtgtccggc ttataaccgg agaagaaatt
     4801 cgagcagatt atatgatcgc tgccggagat ttacgccaag cgctcactca actgacaccg
     4861 gaaacaatgc tcccgaagaa atttaatcga aaattaaaag atgcggaggt ctcacactcc
     4921 gtattcaacg ttttcttggg tatcgaccga cccgtcgaaa gtttaggcct tcaaggttgt
     4981 tcgcatgttt tttatcatcc ggatctgaac ggtatctcgg aaaacgaccg gattcatcgc
     5041 cccgattact ttcgtcatgt tcctcaggag atttccgtcc catgcctcca ccaaaatgag
     5101 cttgcacctc agggtaaaac gggaatgatg atcagcgcaa tgacatcctg gaattacaac
     5161 ggcggttggg aaagagaacc tgcggaatat gagagtttaa aagaaaaata tacatccgat
     5221 ttaatcgccg gtttggaaaa gttcattccc cagctaaacg atcatatcga aatatgtttc
     5281 tcggcgacac cgctcacaat agcccaacgt acgtccaata caaaaggagc cattatgggt
     5341 tggtcttatc acaaggaacg gactttctca cgagggaatt tcttacaaat gaaatcaagc
     5401 gtttttactc ccattcccgg gttgttgacg gccggtcact ggacctattc accaggagga
     5461 tcaccggtag cagtattaac cggaaaatta gctgccgaag cggtattaca tgacgtaaat
     5521 aaaaaggccg aacacaaaaa atgaaacttt atgaaaattt tattttaagg gatcactatg
     5581 atgtaatcgt tgttggcgca ggtctgggtg gaatggcagc agccggatta ttggcaaaac
     5641 gaggtctttc cgttttaatg atcgagcaac aaaacaagcc cggcggagcc tgtacttcat
     5701 ttaaacgaga agaccatatt catgatgtag gagccgccat gttatatggt ttcggtcaaa
     5761 aagggttccg gccgttccat tttttactca atgaactcga agaaccgatt gatatgattt
     5821 cccattccac tttagcccgt atgaactttg aaggaaaaga aattatcttt tggccggatc
     5881 tgcaacgatt tatcgatgaa ttaatcgagt tgtttccgga cgaaaaagaa ggcatcaacg
     5941 ctttttattc agatctttac aaaatgtatg aaaatattgt tattaagaat gaagtcatcg
     6001 tccctccttc cgaatattcc ccgcgtcagg gtttacggcg tctgatcaac gatccgtttt
     6061 cgatatttaa aatgcaaaag ttactatcta cttccaccat ggatcttttg aaaaagtatt
     6121 tccattctcc cgagttattc aatttttacg ataaactttg ctccgcttat gcatatacca
     6181 ccgcagaaga gacgccggcg gttctggctg caacaatgtt tatcgacaat cataccggcg
     6241 gagtttatta tccggccggc ggtgcacaaa tgctcccgaa tacaatcgaa aaagctttcg
     6301 agcgttatgg cggtcaagcc ctttatcgtc aactcgtcga cgagatcctt atcgaagacg
     6361 ggcgcgctta tggtgtacga ctgcaagata aaaccgaaat atttgccgat cgggtgatcg
     6421 ccaacgcaac cgtctggaat atttacggac atctggtacg gccacggcat atttctccgg
     6481 atcgtcttga atgggcgcaa tctttaattc ccacttttcc gagcatgaca ctttacatgg
     6541 ttgtcgatcg ggaggctatt ccggaagatt cttttccttg ggaaatcttc atcgaaaatc
     6601 ggaaaataat tgattcatct gatttgacgt tatacataaa ctcactcgtt gatcactcac
     6661 tttgtccccc ggatgaactg gttgtaatgg caatctcacc gaatttttgc tcttggccgc
     6721 atacggatga tcccacttac cgttcaccgg aatatgaaga tcaaaaacgt agagaagcgg
     6781 agcgaatgat tgatcaaatt gagcaacata ttcccggttt taaacaacac atccgctcat
     6841 taatcatcgg atcaccgacc acgatcgaac gatacttact taaaaacggc ggagcggtcg
     6901 gcggtccaaa aaatcaaatc ggtcaggaaa tgctcaaacg actgcacgca cgcagtgaat
     6961 ggaaaaacct ttatttttgc ggagactcaa ccgtgatggg caccggtgct ccggcaaccg
     7021 tcgtatccgg aatcggcgca gcgaatgtta ttttacgcga attgcatcaa cccgaatatg
     7081 atacccacgt cttccccgaa gaagttgttc attttgttca acctccatat cacagaccgg
     7141 actacatttc cggcacacct gtttcggtcg aaaatgcatt tttggctgca gcgaaatgtc
     7201 agggatgtct tcatccgcga tgccgaaaag gttgccctgc gggaattgat attccgggat
     7261 ttttgcgcag gatggaagca aaaaattaca tcggggcagc ccgcctgatt catgaaaaaa
     7321 atcctttcgg cgaaacctgt ggcttaacct gtgcggcgaa tcatctttgc caacaatttt
     7381 gttatcgccg ggacttcagc ggggaaccgg taaatatcgc cgaacttgag cgatgggttt
     7441 gcgagcaagc gggagaggat ggttggcaaa aacacgaaaa atcgaccgtg aacggggaaa
     7501 tcgccatcat cggcggagga ttggctgcaa tgagctgtgc ttattatctg gctctcagcg
     7561 gatggggaat cagtattttt gcgccggaaa atcaaccggg aggcaaatta tcggaaaaaa
     7621 tgagatccga tccggttctt caagcatctt tcaatcgcga tatccgggga atttttgccg
     7681 aagaaataca ctttttcgga aatcagaatc cggttcatga ggccgatttg gaaagaatgc
     7741 tcaaagaata cgcgtgcatt tttatcacaa aagatcaatt cgcaaagcca gaaaatcttt
     7801 acgaagaaag ggaaaatttt ccggggcttt tcatcggcga tgaattctca gtgaacagta
     7861 ttcctccggt tgaggctgtc gcttgcggtc ggaagacagc cgttgaaatt taccgatatt
     7921 tacgg
//
LOCUS       DAOSMM010000078         7380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_10087_length_7380_cov_18.249693, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000078 DAOSMM010000000
VERSION     DAOSMM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10087_length_7380_cov_18.249693"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..937
                     /locus_tag="PK898_09100"
     CDS             23..937
                     /locus_tag="PK898_09100"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOU20228.1"
                     /translation="MPKRFFDPILILFVFSLAILVCAVFLFPNITNAFTDKDNAFSYL
                     EEQMSFGPRTPGSEAHEKFLNWVTQKLSAQGWDVDLQKGNYKGHEITNVIASRQNLAA
                     DPDAAWIMLGAHYDSRLSADADSVSANQTQPVPGANDGASGVAILLELAERLPKESDK
                     NIWLVFFDGEDQGNIVGWDEWCIGSKMMAQYLEKQDRRPDKVVVIDMVGDKDLQIFRE
                     KNSDKALTDEIWSTAEKEGLSNIFQDKEKYSIYDDHIPFLKIGIPAVDLIDFDYPAWH
                     TLADDIQNVSSDSLAAVEQVLYAWLTKK"
     gene            1112..2824
                     /locus_tag="PK898_09105"
     CDS             1112..2824
                     /locus_tag="PK898_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOU20229.1"
                     /translation="MAIELSNEQKKIFDNVRNTLTELLAVASRSDVPEKDIETLQNTL
                     KSLEDKFLVVIVGEFNSGKSTFINALLNTDILETGVTPTTSLIHLIRYGEEQSSAPIE
                     NWGQLITLPVPLLQSISLVDTPGTNSIFSDHIILTNWFFPRADLVIFITSADRPYTES
                     ESRFLQAIREWGKKIIIILNKKDLNESPEELDRIVRFIQENANRQLKSDIPILTVSAK
                     TALRARKENNPELWKKSGFDQLEIFLQEKLNEKSRFKYKMDSALAIGERVANQTLQLL
                     ENEIKFYREDLNLADAIQEQTQVYRSEIEREINRSLSEIHAYFSQIKESGNEYFEKLF
                     KVKNIPNLIRKDKTKLEFQNEVLKSLPTDIERKTMEMVENIYLQEQRMTQYATNQIEK
                     RKTQFPGTAISSPELSERSQLLQKMQATIDEMLEKMEKDMAADIGMKHVQTAATTALA
                     IEVSAVGLGAGLTIVATTVATDILGIVAAFWVGVAGFMILPYYRKKSQKEFTAQISEI
                     EEKLINSLQKELNEEIQSQTLQMEQAISPFRQFVSNALDKINNQMHETERLRQQIQEL
                     RTKL"
     gene            complement(2843..4102)
                     /locus_tag="PK898_09110"
     CDS             complement(2843..4102)
                     /locus_tag="PK898_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20230.1"
                     /translation="MEKAENLNPDSETYIVPADSLLSDFKFLNSDKTYETNRTPGYPF
                     VIAGAKLIAGDQWKWIVIFFQYGMNILAVMVFYDLLSLLIRNQKAVNLATFLAVINLH
                     DIYFANLILTDSLFQSIILIGIYFFVRFLLQFKTSFLVMAMACMSVAAFIRPSGYYLP
                     FAVLIGVAVITFRNFKQLCKFSLIIFLIGITPLLCWQHRNYRVADFNGFSSITAINLY
                     YYHCAGINAVLNGTDFYTEQENLMNNPELIELQKSMTLPAAEQKLAKEIISAHIPIYI
                     LLNIQGAGFIMLFPGVLDIFRIDDEKLDFLSELKEVSLQKGLKGGIFFLLTDPMGILT
                     ILNEIFLVILAYINLVGMIRSFSYLKPWYVPAALLCFFLYFLCVSAGPNGYGSFPRFR
                     LSISFLQSLYVGIWYAFRQEKKPKILV"
     gene            4574..5695
                     /locus_tag="PK898_09115"
     CDS             4574..5695
                     /locus_tag="PK898_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOU20231.1"
                     /translation="MNTIWILIQFILAFALIVSLHEFGHFLMAKFFKVEVEEFGIGLP
                     PRAAKLFTWGGTDFTLNWLPFGAFVKPKNEFGDDMSEGGFKAAKPWQQFLIYLAGPIM
                     NLLTAFVLYSVIILNIGSPDPSRVIIDQVESGSPAEIAGILPGDQIVQIAGKDINSFE
                     VVTETVDQYLDQEIPIRVLRNGEYIDLKVTPLSNPPEGRGAMGIVITYPMVDYPFFEG
                     VITAARDTGNMIHQYVQGIWQLITGKIDMGIESIVGPVGMFSYYEEAATLDEQAVLEN
                     EQKTNESATQTQSSRTTSSGLNRLAFFVVVSIALGITNLFPIPALDGGQILLLMPQLL
                     FKKKLSPKVEYYFNAIGMACLLLLMAVIMFKDIFMLANG"
     gene            5698..6987
                     /locus_tag="PK898_09120"
     CDS             5698..6987
                     /locus_tag="PK898_09120"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOU20232.1"
                     /translation="MKQEKNNQNKKNKFSDILSELRENNRELTYKEIQSFSDLSEENL
                     AQFRQTWNLLSNKRKEMFFELLLVEFMSNTLMDFSEIALIGLNDEDPIIRRGSLKLLM
                     DNRKSYFLDRLISISKQDPDTEVRLDAISTLGYFLMDTDTAERGKNKAEKVLKTLESL
                     MESPDKMTRLRVMEALAYVDHPSIIPLVYASLTSDIDTEIASGLRAVQNSLNRRWAAN
                     VIENLDHPNPDVQYEAIKAVGELQLKRARNRILSLLARFDQLDDDILDATILTASQLG
                     GNQVKEMIEMIEEIFEDEEDMAELFEEAKSNLELVDFQKQMAGNPDLKDLFTEESDEE
                     SENVEEDYIQILRERIENLPECEESEDKFEEIEEENEEEEDEDESDYPNRHSHRHSSF
                     SDIDWSHFRIIEDLRKESEDLEDEDFSDLDEFEEDED"
     gene            7197..>7380
                     /locus_tag="PK898_09125"
     CDS             7197..>7380
                     /locus_tag="PK898_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOU20233.1"
                     /translation="MAKRRALGIDVSHWQGNVDFKKAKKAGASFAIIKASQNVWYDDK
                     FQQNWANAKDAKIIRGA"
BASE COUNT         2431 a         1452 c         1573 g         1924 t
ORIGIN      
        1 cttatctgat aaggaaaatt gcatgccgaa aaggtttttt gatccgattc tcattttatt
       61 tgttttttct ctcgctattt tagtttgtgc ggttttcctt tttcccaata tcaccaatgc
      121 tttcaccgat aaagataatg ctttttccta tttggaagaa caaatgagct ttggtccccg
      181 aacaccgggt tccgaagccc acgaaaaatt tctgaactgg gttacccaaa aattatccgc
      241 ccaaggttgg gatgtcgatt tacaaaaggg aaattacaaa ggccatgaaa tcacgaatgt
      301 gattgcatcc cgacaaaatt tggccgcgga cccggatgcc gcatggatta tgctcggagc
      361 gcattatgat tcgcgtcttt ccgccgatgc cgactccgtc tctgcgaatc aaacacagcc
      421 ggttccggga gctaatgacg gcgcatccgg agttgccatt ttgctggaat tagctgaaag
      481 actgccgaaa gaatccgaca aaaacatctg gctggtattc ttcgatggcg aggatcaggg
      541 aaatatcgta gggtgggatg aatggtgcat cggctcgaaa atgatggccc aatatctcga
      601 aaaacaagat cgccggccgg ataaagttgt ggtgattgat atggtcgggg ataaagattt
      661 acaaatcttc cgcgaaaaaa attccgataa agcattaacg gatgaaatct ggtcaaccgc
      721 cgaaaaggaa ggattgagca atatttttca agataaagaa aaatacagca tatatgatga
      781 ccatattcct tttctgaaaa tcggaattcc ggctgtcgat ttaatcgatt ttgattaccc
      841 tgcctggcac actttagccg acgatattca aaatgtgtct tccgatagtt tggctgcggt
      901 tgagcaagtg ctttatgcct ggctcacaaa aaaataaacc aaaacataaa acagttagat
      961 tctctttatt cgctgcatcc ctattttgct ttatttaaga attcacagaa ccggttatcc
     1021 gaagcatagc gccgaattat gactcacgaa caatgccgtt tagcgaaatg ctatataatt
     1081 ccttcataac aggtgaagga aagaccaaaa aatggcaatt gagctttcaa acgaacagaa
     1141 aaagattttt gataacgtcc ggaatacatt aacggaatta ctcgccgtcg catctcgcag
     1201 cgatgtcccc gaaaaagata tcgagacgct gcaaaatacc ttaaaatctc tcgaagacaa
     1261 attcttggtg gtcattgtcg gagaatttaa ttccgggaaa tcaacattta tcaatgcgtt
     1321 gttaaatacc gatatacttg agaccggcgt cactcctact acttcattga ttcatctgat
     1381 tcgttacgga gaagaacaat cttctgctcc gattgaaaat tggggacaat tgattaccct
     1441 gccggtccca ttgctgcaat cgatcagcct ggtagacact cccgggacaa attcgatttt
     1501 ttcggatcat atcatcctta ccaactggtt ctttccaaga gccgatctgg tcatttttat
     1561 tacctcggcg gatcgtcctt atacagaaag tgagagccgg tttcttcaag cgattcggga
     1621 gtgggggaag aaaataatta ttattctgaa taaaaaagat ttaaacgagt ctccggaaga
     1681 gcttgatcga atcgtccgtt ttattcaaga aaatgcgaat cgccagctta aatcggacat
     1741 accgattctc actgtcagcg cgaaaaccgc tttacgggca cgtaaagaaa ataatccgga
     1801 attatggaag aaaagcggct tcgatcagct cgaaatcttt ctccaagaga aattaaatga
     1861 aaaatccaga tttaaatata aaatggactc cgccttggca atcggtgagc gagtggcaaa
     1921 ccaaaccctg caactgctgg aaaacgagat caaattttat cgggaggatc tcaacttggc
     1981 tgatgccatt caagaacaga cgcaagtcta ccgatctgaa attgaaagag aaatcaaccg
     2041 ttctctgagc gaaattcacg cctatttcag ccaaatcaag gaaagcggaa acgaatattt
     2101 cgagaaactt tttaaagtga aaaatattcc caatttgatc cgcaaagata aaaccaagtt
     2161 ggaatttcaa aatgaagttt taaaatctct tcctacggat atcgaaagaa aaacgatgga
     2221 aatggttgaa aatatttatt tgcaagaaca gcgcatgacc caatacgcaa ccaaccagat
     2281 agaaaaaaga aaaacccagt ttccgggaac agccatttcc tctccggaac tttcggaacg
     2341 ttctcagctg ttacaaaaaa tgcaagccac gatcgatgaa atgctggaaa agatggaaaa
     2401 agatatggca gcggacatcg gaatgaaaca tgtacagaca gctgccacca cagcgcttgc
     2461 aatcgaagtt tcggcggtag gccttggggc gggtcttaca attgttgcca ccaccgtcgc
     2521 aaccgatatt ctaggcattg tggctgcctt ttgggtcggg gtagccggtt tcatgatatt
     2581 accttattac cgaaaaaaga gtcaaaaaga atttaccgct cagatttcgg aaatcgagga
     2641 aaaactgatc aactcactgc agaaagaact caacgaagaa atacagtctc aaacacttca
     2701 aatggaacag gctatttcgc ctttcaggca gtttgtcagc aacgccttgg ataaaatcaa
     2761 taaccaaatg cacgaaacgg aacgcctgag acaacagatt caggaactga gaacaaaact
     2821 ttagaaattc atcggataaa atctacacaa gaatttttgg tttcttttcc tgccgaaaag
     2881 cataccaaat accgacataa agagattgga gaaacgaaat agataatcgg aaccgaggaa
     2941 acgatccgta tccgttgggt cccgcactga cgcacagaaa ataaaggaaa aaacaaagca
     3001 aagccgccgg cacataccac ggtttcagat aggaaaaaga tcgaatcatt ccgaccaaat
     3061 taatatacgc caaaatcacc aggaagattt cattgaggat tgtaagtatt cccatcggat
     3121 cggtcagcaa aaaaaagatg ccgcctttca agcctttttg taaagaaact tcttttaatt
     3181 cggaaaggaa atccaatttt tcatcatcga ttctgaagat atccaacaca cccgggaaaa
     3241 gcataataaa tccggcgcct tgaatattca acaaaatata gatgggaata tgagccgaga
     3301 tgatttcttt ggctaatttt tgctcggcag ccggtaaagt catcgatttt tgaagttcga
     3361 tcaattccgg attgttcatt aaattttctt gttcggtata gaaatccgtc ccgtttaaaa
     3421 cggcattaat ccccgcacaa tgataatagt acaaattaat tgcagtgatc gaagaaaaac
     3481 cattaaaatc agccacccga taatttcgat gttgccaaca caacaaaggg gttattccga
     3541 ttaaaaatat aatgagagag aacttacaaa gctgtttgaa attccgaaat gtgataactg
     3601 ctacgccgat taaaaccgca aaaggcagat aatacccgga aggccgaata aaagcggcaa
     3661 cggacatgca agccattgcc ataacgagaa aagaggtctt gaattgcaaa agaaatcgga
     3721 cgaaaaaata gataccgatc agtataatcg actggaacaa agaatcggtc aaaataagat
     3781 tcgcaaaata aatatcgtga agattaatga ccgccaaaaa tgtggcaaga ttcacggctt
     3841 tttgattccg gatcaataaa gagagcaaat cataaaaaac cattacagcc aggatattca
     3901 taccgtattg gaaaaatatg acgatccatt tccattgatc gcctgcgatc aactttgctc
     3961 ctgcaataac gaagggatat ccgggcgttc gattcgtctc atatgtttta tcggaattga
     4021 gaaatttgaa atcagataat aaagaatctg ccgggacgat ataggtctcg gaatccgggt
     4081 tcaaattttc agctttttcc aatgaaaaac ctgatgcgaa caaaaataac aggaagaata
     4141 ctcgaataaa aatcgcaagg atcaaaaaaa cggttttagg agatctttga tagaaatgct
     4201 ttaattgaac catattcaaa aatgaggatg cttctccgtt tccttccatg attatttttg
     4261 gagcatggcc actttataaa agtccaatat gaaatatcga taaaaagaaa ccggctccat
     4321 tttaattcgc gtttgtcttt tccgttattc cggatcaatt ttaccagcgg atccacaaat
     4381 tgagaccttt taccatgaga acagaaagaa gttcaattca aaagatatct taatttcgca
     4441 ttaattatcc tctaatactt tcacggtaca ataaatcaag atttataata taaattctgc
     4501 cgattcaaaa atcggaccgg cggtaagaaa atagcaataa aagaaaatag cacgtcgcta
     4561 tttatggagt aggatgaaca caatttggat cttaatacaa tttatcttgg cattcgcact
     4621 tatcgtatcg ttgcatgaat tcggacattt tttgatggca aaatttttta aagtcgaagt
     4681 tgaagaattc ggcatcggac tgccacctcg ggcagcaaaa cttttcacat ggggaggaac
     4741 cgacttcacc ctgaactggt tgccatttgg agcatttgtc aagccgaaaa acgaattcgg
     4801 cgatgatatg agtgaaggcg gatttaaagc cgctaaacca tggcagcaat ttttaatcta
     4861 tctcgcgggt ccgattatga atttattgac cgcttttgta ctgtatagcg tcattattct
     4921 taatatcggc tcaccggatc cgagtagagt aatcatcgat caggtcgaat cgggttctcc
     4981 ggcagaaatc gccggaattc tcccgggaga tcaaattgtc caaattgccg gtaaagatat
     5041 caactctttt gaagttgtta cagagacggt tgatcaatat ctggatcagg aaattccgat
     5101 tcgagtgctc cgaaacgggg aatatatcga tttgaaggtg actccgctga gcaatccacc
     5161 ggaaggtcgc ggagcaatgg gaatcgttat cacctatccg atggtagatt atccgttttt
     5221 tgaaggggtc attaccgccg cccgagatac gggaaacatg attcatcaat acgttcaagg
     5281 catctggcaa ctgataaccg gaaaaattga tatgggcatt gaatccattg tgggaccggt
     5341 cggaatgttc tcatattacg aggaagctgc cactttagat gaacaagctg tgcttgaaaa
     5401 cgagcagaaa acaaatgaat ccgccactca aacccaaagc agcaggacaa catcatcagg
     5461 attgaatcgt cttgcatttt tcgtggtggt atccattgcg ctgggaataa ccaatttatt
     5521 cccgattccg gcgctggacg gaggacaaat tctgcttctg atgcctcaat tattattcaa
     5581 gaaaaagtta tcacccaaag tcgaatacta tttcaatgcg atcgggatgg cttgcctgct
     5641 gttattaatg gctgtaatta tgtttaaaga tatcttcatg cttgcaaacg gataaaaatg
     5701 aaacaggaaa aaaacaatca aaataaaaag aataagttca gcgatattct ttccgaatta
     5761 agggaaaata atcgtgaact gacttacaag gaaatacaat ccttttccga tctttcggaa
     5821 gagaatttgg cacaatttcg tcagacatgg aacttgcttt caaataaacg aaaggaaatg
     5881 ttctttgaat tgctgttggt tgaattcatg agcaatacct taatggactt ttccgaaatc
     5941 gctttgatcg gactgaatga tgaagatccg attattcgtc gcggatccct gaaacttctg
     6001 atggacaatc gaaaaagtta ttttttagat cggcttatct cgatttcgaa gcaggatccc
     6061 gatacggaag ttcgattgga cgcaatcagc acactcggat atttcctgat ggacacggat
     6121 acggctgaac gagggaaaaa taaagccgaa aaagtgctga aaacgctgga atcactgatg
     6181 gaatcaccgg ataaaatgac acggctcaga gttatggaag ctctggctta tgtcgatcat
     6241 ccttccatta ttcctttggt ttatgccagc ctgacaagtg atattgatac ggaaattgcc
     6301 tcaggtttgc gtgcggttca aaattctctt aataggagat gggctgcaaa tgtaattgag
     6361 aatctggatc atccgaaccc cgatgttcag tatgaagcca tcaaagccgt cggagaattg
     6421 caactgaaac gggcgagaaa tcgaattctc agcctgttag cacggttcga ccagttggat
     6481 gacgatattt tggatgcgac gatcctgacc gcttcccaat tgggaggaaa ccaagttaaa
     6541 gaaatgattg aaatgatcga ggagattttc gaagacgaag aggatatggc ggagcttttt
     6601 gaagaagcca aatcgaacct ggaactggtt gattttcaaa aacagatggc aggcaaccct
     6661 gatttgaaag atctttttac tgaagaatcg gatgaggaat cggagaacgt agaggaagat
     6721 tacattcaaa tattacggga acggatcgaa aacttgcccg agtgcgaaga gtccgaggat
     6781 aagtttgagg aaattgagga agaaaacgaa gaagaagagg atgaagatga atcggattat
     6841 ccgaatcggc attctcatcg ccactcctca ttttccgata ttgattggag tcatttccga
     6901 attatcgagg acttgagaaa ggaatccgaa gatttggaag atgaggattt ttccgattta
     6961 gatgaatttg aggaagatga agattaaact gacaataaat taagtcgtct tttcggaatc
     7021 ttcaaatcaa aaaagagcca tttcttgttc ggctcttttt tggtatatca atggcgttgt
     7081 ttctaaaaaa cgatcgaaat cggaaaatac tttttatctt tctttggcac actttttgca
     7141 acttagttta aagcagataa agcgacagaa gcaagatcgt tcggaaggga gggcgcgtgg
     7201 cgaaacgcag agcattaggg atcgatgtca gccattggca agggaatgtc gattttaaaa
     7261 aagccaaaaa agccggagca tcttttgcga ttattaaagc cagccaaaac gtatggtacg
     7321 atgacaagtt ccaacagaat tgggcaaatg ccaaagatgc aaaaataatc cggggcgctt
//
LOCUS       DAOSMM010000079         7027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_10759_length_7027_cov_18.288583, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000079 DAOSMM010000000
VERSION     DAOSMM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10759_length_7027_cov_18.288583"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1027)
                     /gene="galE"
                     /locus_tag="PK898_09130"
     CDS             complement(14..1027)
                     /gene="galE"
                     /locus_tag="PK898_09130"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOU20234.1"
                     /translation="MKFLVTGGAGYIGSTVVSALEDAGHQAVILDSLVTGRIEFTRGK
                     IFYLGDIADRKILTQIFADHPDIFAVLHCAALIVVPESVEKPYEYYRENVAKSNEMFH
                     ILYTLGCQRIVFSSSAAIYDKAENFMVTEDSPLAPNSPYSRTKYMMEMILQDTSNAYN
                     MRGIALRYFNPIGADPKMRSGMHVKEPSHVLGKLVDTALGKQDAFTVTGTNWPTRDGS
                     GIRDYIHVWDLAQAHVKSLAEFDQVFERTGDPNINFIPINLGSGKGVTVFELVNAFER
                     VYGKKINIRTAPPRPGDVAGAYANADRAKKLLGWETQLSIEDGIRTALEWGKIRESIL
                     HWD"
     gene            complement(1024..1995)
                     /locus_tag="PK898_09135"
     CDS             complement(1024..1995)
                     /locus_tag="PK898_09135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU20235.1"
                     /translation="MKLISIIIPMFNEEASCGGFYDELVPVINSLPYRFELIFVNDGS
                     VDRTQQILESILEKDRRVQIIQLSRNFGHQAALCAGLENASGDYIITMDGDGQHPPRL
                     IPEMIQLAENGYDIVQTQRLDDEEKSSFKKRTSKWFYQLINQIGDTKTLPGGADFRLI
                     TKQVLDNLLAMPEYHRFLRGMVSWLGFRSVVIPFHPEERLAGESKYSLKKMLKLGSDA
                     IFSFSLVPLYVGLSAGGIFFILAVIEVIYVLSFWISGRSAELTPGWSSLMFMMLIIAA
                     VLMILLGFIGIYVGYIFQEVKHRPVFVIKEKKVHPNNQKEQSEEENL"
     gene            complement(2239..4476)
                     /locus_tag="PK898_09140"
     CDS             complement(2239..4476)
                     /locus_tag="PK898_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU20236.1"
                     /translation="MNYLENLNPQQYQAVTAGEGSVLVLAGPGSGKTRVLTQRIAYLI
                     KEMGVPAYNILAVTFTNKAAREMQDRVEDLLGESIQGNMWLGTFHAVCARILRREASL
                     LPFGHNYVIMDADDQVTLIKQIIKELNLDDKIFRPNSVHNEISNAKNNLLFPQDMLRS
                     NYREETVFRVYERYQQALEKSNAVDFDDLLLWCWKLLKDNPDIRDRYSRRFRHILVDE
                     FQDTNTAQYQLLKLLAGQKGNIFVVGDEDQSIYRWRGADYHNIQLFEKDYPNCTKILL
                     EQNYRSTQNVLNVARSVIDHNPNRTRKNLFSDRGSGDKIVYYEAEDDRMEARYVVETI
                     SKLIENGNARGGDFAVLYRTNAQSRLLEDAFLYRGMSYRLVGAQRFYGRREVKDMIAY
                     LRLAYNPRDEISLARVINIPPRGIGEKSFQNLRMAALESGKASGDVLLDLGKYGPESV
                     FWPDMGRGALPLADFGARLLEWSSDPLISGGITMLLDKIIADTRYQDYLKADGDDESD
                     RWENVEELRKMAFEYEDRGLADFLQNLALVSDQDTMTNNTDVPTLMTLHAVKGLEFNR
                     VFIIGLDDGLLPHNRSFDDPEEMAEERRLFYVGLTRARNHVTLVRAQQRMAFGVPDIT
                     IPSRFLTYIPEELIIRENESSKESRRSSGSYHSGSRWESTPSWASTFRSGPPSASQSM
                     SKNSNGEVRQIFSPQDNVNHPLWGEGTILESKIEDGEEVVQVQFKSSGIKKLIVSLSK
                     LKKIV"
     gene            complement(4473..5579)
                     /gene="mutY"
                     /locus_tag="PK898_09145"
     CDS             complement(4473..5579)
                     /gene="mutY"
                     /locus_tag="PK898_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOU20237.1"
                     /translation="MNKPMHQELLEWFAKNKRDLPWRENRTPYRVWISEIMLQQTRVE
                     TVIPYYTRWMEAFPDPVSLAKSDEQQVLKQWEGLGYYGRARTLYKTAGIIMQKWNGIF
                     PNDLKTLESLPGIGRYTAGAIASIAFNQAVPALDGNIRRIYSRLFDLAVPLRTKESET
                     ILWQLAGSEIEGENSPGAYHEALMDLGSSVCLPSAPRCLLCPLTSWCEAFKKNTVSQR
                     PVMKQTKSIPHHTVTAGIIRKPNDEVLIAQRPAHGLLGGLWEFPGGKQEKDETLEECL
                     IRELWEELEVQVIIKAPFGVYKHAYTHFRVTLHAFLCEIGDQNPQPVEAQALRWVEIP
                     NLAAFPMGKIDRQIARDLQKIEPIRNGEESIDNL"
     gene            complement(5836..6138)
                     /locus_tag="PK898_09150"
     CDS             complement(5836..6138)
                     /locus_tag="PK898_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20238.1"
                     /translation="MPVKLIMTWDILPNHEQEYFDFLVREFVPSLNRLGFELSDAWAT
                     IYGDQPQVQISALLPNESEAEQRMAKPEWMELKEKLQQFIQNFECKLVPARQGFQF"
     gene            complement(6128..6826)
                     /locus_tag="PK898_09155"
     CDS             complement(6128..6826)
                     /locus_tag="PK898_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU20239.1"
                     /translation="MEITWYGHSCFRITERGMGTVVCDPYDSQEAGFEPLKLKADVVT
                     CSSNHPGHHFISAVKGDPFIIDSPGEYEINNVFINGYRSGETDGVKNTIFVIEYNGLF
                     VAHLGNLNRIPSLSEIETYGTIHVLLIPVGGGGALNSSKAVELISQIKPNIVIPMHYA
                     DPLGKQELDPLSKFLKEMGISQTEDVYNSLKITNATQLPEETKLIILNHPLSDSLNDQ
                     DDSAEDNNEEVNAG"
BASE COUNT         1877 a         1662 c         1501 g         1987 t
ORIGIN      
        1 ttagttcagg cgattaatcc caatgtaaaa tactctctcg tatctttccc cactccaagg
       61 cggtgcgaat tccgtcttca atgctcagtt gagtttccca acccagcaat ttttttgccc
      121 gatccgcatt ggcgtacgct cccgcaacgt ctccgggacg tggcggcgct gtacgaatgt
      181 taatcttttt cccataaacc cgttcaaaag cattcaccag ttcaaaaacg gttacgcctt
      241 tgccggaacc gagattaatg ggtataaaat tgatgttcgg atcgccggtt cgttcaaaaa
      301 cctgatcaaa ttcagccaac gatttgacat gggcttgcgc cagatcccaa acgtgaatgt
      361 aatctcgaat acccgagccg tctcgtgtcg gccagtttgt acccgtaacg gtgaaagcat
      421 cctgtttgcc caatgccgta tccaccaatt ttccgaggac atgagaaggt tccttgacat
      481 gcatgccact gcgcatcttc ggatcggctc cgatcggatt gaaataccga agtgcgatcc
      541 cccgcatatt ataagcattg gaggtatcct gcaaaatcat ttccatcata tattttgttc
      601 tggaataggg actgttggga gccagcggcg aatcctcggt caccatgaaa ttttccgctt
      661 tatcataaat ggcggccgaa gaactgaata cgatacgttg gcaacctaag gtatagagga
      721 tatgaaacat ttcattggat tttgcgacat tctcacgata gtattcataa ggcttttcaa
      781 cggattccgg aacgacaatc agagccgcac aatgtaacac ggcaaaaata tccggatgat
      841 ctgcaaaaat ttgcgtcagg attttgcgat ccgcaatatc tcccaaataa aaaatctttc
      901 cgcgggtaaa ttctattctg ccggtaacaa gggaatctaa aatcacagct tgatgccccg
      961 catcttcgag ggcagaaacg actgtcgatc cgatataacc ggctccgccg gtaactaaaa
     1021 atttcataaa ttttcctctt ctgattgttc tttttgatta ttcggatgaa cttttttctc
     1081 tttgatcaca aaaaccggtc gatgttttac ttcctggaaa atatatccga cataaattcc
     1141 gataaatcca agcaaaatca tcaaaaccgc ggcgataatc agcatcataa acatcaacga
     1201 gctccagccg ggcgttagct ccgccgatct acccgaaatc cagaatgaca ggacataaat
     1261 aacttcgatt acagccagaa tgaagaatat gccgccggct gaaagtccga cataaagggg
     1321 gacaagtgaa aaagaaaaga tcgcatccga accgagtttg agcatttttt tcagagaata
     1381 ttttgattct cccgccaacc tttcttcggg atgaaaaggt atgacgaccg atcgaaaacc
     1441 caaccatgac accatgccgc gcaaaaaacg atgatattcc ggcatcgcca gtaaattatc
     1501 caatacctgt tttgtgatca aacgaaaatc ggctcctccg ggtaacgttt tggtatcacc
     1561 gatttgattt atcaactgat agaaccattt ggaagttctt tttttaaagg agctcttttc
     1621 ttcatcatcc aatcgttgag tctgaacgat atcatagccg ttttccgcta attgaatcat
     1681 ttccggaatg agacgcggcg ggtgttgtcc gtcgccgtcc attgtaatga tgtagtcgcc
     1741 ggaagcattt tccaaccccg cgcaaagggc tgcctgatga ccgaaattac gacttaactg
     1801 aatgatctga acccggcggt ccttttccaa aatcgattca agaatttgtt gagtacgatc
     1861 gacagatccg tcattgacaa aaatcagctc gaaacgataa ggcagcgaat tgattaccgg
     1921 taccaattca tcgtagaagc cgccgcagga tgcttcttcg ttaaacatcg gaatgattat
     1981 tgagatcagt ttcatgaatt attctgagga ttccgttccc tgaatgaccg gatcgtgcgc
     2041 tttcttccgg tgattatact atgagagaaa cgaaccgatt cggaaaaatg tgcttttaat
     2101 ataaaggaac tcggaatatc gaacgttcta agaaaaattg tctcatatgc ttgtatagag
     2161 aatacagctg ttttactgaa aaaaacgaat ttgatctgtt taaccggtat tgagggttcg
     2221 ttattgaact ctctcggttt aaacgatttt ttttaatttc gacagtgata cgatcagctt
     2281 ctttatcccg gaagatttaa attgcacttg taccacttct tcaccgtcct cgattttact
     2341 ttcgagaatg gtgccttctc cccataaagg atggttcaca ttgtcctgag gagaaaaaat
     2401 ttgccgaact tctccgttac tgtttttcga catggattgg gatgctgaag ggggacctga
     2461 tcggaaggta ctcgcccagg agggggtact ttcccaacgc gatccgctat gatatgatcc
     2521 cgatgatcgc cggctctctt tggaagattc attttctcgg ataatcagtt cttccggaat
     2581 atatgtcaga aatcgggaag ggatcgtgat atccggcact ccgaatgcca ttcgttgttg
     2641 cgcacgcacc aaggttacgt ggttgcgggc gcgagtcaga ccgacataaa ataatcgcct
     2701 ttcttccgcc atttcctccg gatcgtcaaa gctgcgatta tgtggtaaca gtccgtcatc
     2761 taaaccgatg ataaaaaccc gattaaattc caaacctttt acggcatgca atgtcattaa
     2821 tgtggggaca tcggtgttgt tcgtcatcgt atcctgatcg gaaacaagtg ctaaattttg
     2881 caggaaatca gccagcccgc gatcttcata ttcgaaagcc attttacgca attcttcaac
     2941 attttcccaa cgatctgatt catcatcacc gtcggctttc agataatcct gatatcgggt
     3001 atcggcgata attttgtcca gcagcatggt gataccgccg gaaatgagcg ggtcggaact
     3061 ccattcgagt aggcgtgcac caaaatctgc caacggcaaa gcgccccgtc ccatgtcggg
     3121 ccaaaacacg gattcgggac cgtatttccc gaggtccaac agaacatctc cggaagcctt
     3181 tccgctctcc agtgccgcca ttcgcagatt ttggaaggat ttttctccga tacctcttgg
     3241 cggaatgttg attacccggg caaggctgat ttcatcccgc ggattatacg ccaaccgcaa
     3301 atatgcgatc atatccttta cttctcgtcg accgtaaaag cgttgtgcac cgaccagccg
     3361 ataagacatt ccgcgatata aaaatgcatc ttccaacagc ctggattgag cattggtccg
     3421 atataaaaca gcgaaatctc ctccccgagc atttccgttc tcaatcaatt tcgaaatggt
     3481 ttcaaccaca tatcgcgcct ccattcggtc gtcttccgct tcgtagtaaa cgattttatc
     3541 ccccgacccg cggtcggaaa acaggttttt ccgggtgcga ttggggttat gatcgatgac
     3601 cgaacgggcg acgttcaaga cattttgcgt ggaacgataa ttttgttcaa gcaatatctt
     3661 ggtgcaattc ggataatctt tttcaaataa ttgaatattg tggtaatccg caccacgcca
     3721 acggtaaatg gattgatctt catctccgac aacaaagata tttccttttt gaccggcaag
     3781 cagttttaac aactgatatt gtgccgtatt ggtatcctga aattcatcga ccaaaatatg
     3841 gcggaatctg cgtgaatagc ggtctcgaat atccgggttg tctttcaaca gtttccaaca
     3901 ccataaaagc aaatcatcaa aatccacggc attgcttttt tcgagtgctt gttgatatct
     3961 ttcgtaaact ctgaagacag tttcttcccg atagttgctg cgcagcatat cttgaggaaa
     4021 caaaagatta ttctttgcat tggaaatctc gttatggacg gaattcgggc gaaagatctt
     4081 atcatccaga ttcagttcct taatgatctg tttgattaat gtcacttgat catctgcatc
     4141 catgatgacg taattgtggc cgaaggggag cagagaagct tcacgtcgta agattcgcgc
     4201 gcaaactgcg tgaaaggtac cgagccacat attgccctgt atgctctcgc ccagaagatc
     4261 ttcgacacga tcctgcatct cgcgtgccgc tttatttgta aaagtaaccg ccaatatatt
     4321 gtaagccggg actcccattt ccttgatcag gtaggcgatt cgttgtgtca gcacccgggt
     4381 cttccccgaa ccgggcccgg ccagtaccaa aacacttccc tctccggcgg taaccgcctg
     4441 atattgctgt ggatttaagt tttctaaata attcataggt tatcgattga ttcttcccca
     4501 tttcggatcg gttcgatttt ctgtaaatcc cgagcgattt gccggtcaat tttccccatc
     4561 gggaaagcag ccagattcgg aatctctacc catcgcaggg cttgagcttc aacgggttgc
     4621 ggattttgat ctccgatttc gcataaaaaa gcatggagag tgacccgaaa atgagtatag
     4681 gcatgtttat ataccccaaa gggcgctttt attataactt gcacttccag ctcttcccaa
     4741 agttcacgga ttaaacattc ttcgagcgtt tcatcctttt cctgctttcc acccgggaat
     4801 tcccaaagac cgcccaataa tccgtgtgcg ggtctttgag cgatcaatac ttcatcgttc
     4861 ggttttcgaa tgatgccggc ggtaacggta tgatggggaa tggatttcgt ctgtttcatc
     4921 accggtcttt gcgaaaccgt attctttttg aaagcttcgc accaacttgt caaggggcag
     4981 agcaaacatc ggggtgcgga cggcaggcag acggaagaac ccaaatccat caatgcttcg
     5041 tggtaagcac cgggggaatt ctctccttca atttccgaac cggcgagctg ccaaagaatc
     5101 gtttccgact cctttgtccg aaggggaacg gcaaggtcaa acaaacggga gtaaatccgg
     5161 cgaatgttcc catccaatgc gggaaccgct tgattgaaag caatggatgc gatcgcaccg
     5221 gcagtatatc gtccgattcc ggggagactt tcaagcgtct ttaagtcatt cgggaaaatt
     5281 ccattccatt tttgcatgat tatcccggca gttttgtaca gcgttcgggc tcgaccgtaa
     5341 tatcccaacc cttcccattg tttcagaacc tgctgttcgt ccgatttcgc aagcgacacc
     5401 gggtcaggaa aggcttccat ccaacgggta taatagggaa tcaccgtctc gacgcgggtt
     5461 tgttgtaaca tgatttctga aatccacacc cggtaaggtg tgcgattctc ccgccacggg
     5521 agatctcttt tatttttcgc gaaccattct aaaagttcct gatgcatcgg tttgttcatt
     5581 attgtttctt cgatatattt gtcccaaatt gataatcggt tctgcaaata gaattctttt
     5641 tttcatcgag acaggccagt tagattttgc cgaaatattg attgatctgc ttttatgagt
     5701 cgaggcttta acctttaata agggcgattt tcttctctga gcaatttatt aatctcgatc
     5761 gagtgaatga ttcatcttcc gaacaaaaat gcgggagcaa gcccccgcat tcgatcattg
     5821 aaaagctttt tgagatcaga actgaaaacc ttgcctggcg gggaccaatt tacattcaaa
     5881 attttgaata aattgctgca atttttcttt cagctccatc cattccggtt ttgccatccg
     5941 ctgttcggct tccgattcat tgggtaacag agcgctgatc tgtacctgtg gctgatctcc
     6001 gtaaatggtt gcccaagcat ccgaaagttc aaaacctaag cggtttaagc tgggaacaaa
     6061 ttcccgaacc agaaaatcaa aatactcctg ctcatgattt ggcagaatat cccaggtcat
     6121 gattaattta accggcattt acttcctcgt tgttgtcttc tgcggaatca tcctgatcat
     6181 tcaatgaatc gctgagagga tgattcaaaa taatcagttt ggtctcttcc ggaagctgag
     6241 tggcatttgt gatttttaac gaattataaa catcctccgt ttgtgaaatc cccatttctt
     6301 taagaaattt agataatgga tccagttcct gtttccctaa aggatcggca taatgcatcg
     6361 gaatcacaat attaggttta atttggctga tcaattcaac ggctttagaa gaattgagcg
     6421 caccgccgcc tccgaccgga ataagcagca catggatcgt accgtatgtc tcaatctcag
     6481 aaagagacgg tattcggttc agattgccca aatgagcgac gaataaaccg ttatattcaa
     6541 tgacgaaaat ggtattttta actccgtcgg tctcgccgga tcgataacca ttaatgaaaa
     6601 cattattgat ttcatattct ccggggctgt cgatgataaa cggatcccct ttcacagccg
     6661 aaataaaatg gtgccccgga tgattactgc tgcaggttac cacgtcggct tttaatttta
     6721 agggttcaaa accggcttcc tgtgaatcgt aggggtcaca aacaacagtg cccatccctc
     6781 gttctgtgat tcgaaaacag gaatgaccgt accaagtgat ttccatgaat tctcctgaat
     6841 gaatctagat ttaatcttaa cagatatatt ataccctgtg tcgaggaatg aaaatgaaaa
     6901 aagaagttcc ggtgaagagg aagcgacgga acatgagagt ccccgagggt ttgttcactt
     6961 tcctcgatca tcattcgaca gcttcaattt tgcgcttcag gcattcgaat catccggatt
     7021 tccgttt
//
LOCUS       DAOSMM010000080         6918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_11004_length_6918_cov_18.109573, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000080 DAOSMM010000000
VERSION     DAOSMM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11004_length_6918_cov_18.109573"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      150..325
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            408..1808
                     /locus_tag="PK898_09160"
     CDS             408..1808
                     /locus_tag="PK898_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOU20240.1"
                     /translation="MDEKQRLEALTSEKTEFRREIGVFGGVSIIGGIMIGSGIFYLGS
                     YVLIRTGMSQGLSLLAWVLGGLISLLGGLCFAELGASIPRAGGMTVYLNEAFHPLIGF
                     LNAFSGLLIGSPGSIAAIAIALPTAMRTFFPISDSGIKIIAILLIVGLTVFNYFGIRP
                     GSRLQSASMIAKLIPIGIILFASLFLGKVSPDLSMVPEAGPVGFGKLVSMVAFAVIAT
                     LWAYEGWTNLNNVTEEVKNPRRNLPLALIIAIGGITVLYTLFNYGIYRVLPFDQIKAF
                     IESGNYYLGTEAAKATLGNAGAVVVTVAMVLSMFGSLNGCILAFPRTYYAMAEEGHFF
                     HAFAELHPKYKVPATGLIVQCIISVILVLVRNLDQLTSLVIFTSVLYNMLTVLAVIRL
                     RKKYPDIPRPFKIWGYPFTVILTALIFLGLVINTFIEDPVSAITGMVIPVIGTVVYFL
                     FDRYRKKEAAAAAQKN"
     gene            1829..3457
                     /locus_tag="PK898_09165"
     CDS             1829..3457
                     /locus_tag="PK898_09165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU20241.1"
                     /translation="MRTAIYNGKIYLDRGEFAEAVLIEGDTVLQVGTTAEILASPDLS
                     EKIDCEGRTVIPGLNDSHMHLMMIGEALEQLSLENCKSVAELITASKAYLAQHPEAAK
                     KGLYGRAWNQDYFIDEKRIPNRHDLDQISTEIPVVLERICGHILVTNTKAIEMLGLKA
                     GSPQYKGGVFHLEEDGSPNGVFTEDACRYPLGLIQESTLEEKRMLLLKAMEYAVSRGI
                     TSVQSNDLSYYIKDHNGFIRMIHSLYEDEVAPLRYRLQACFQSPEAFAESFENGLYSH
                     ASDLSDDYFAIGPLKLFKDGSLGARTAMVRHPYLDDPENPDNRGVETTDAATMDRYCE
                     IAAKHGVQVVTHVIGDRAVGDTLDSYEKVLIDGENKYRNSLIHCQLTTLEDLKRIARM
                     GILVQYQPIFLNYDHQICEARVGKELASTSYAFRTLQDLGGKISYGTDSPVEDCNPFE
                     NIYCAVTRKGLDGQPPEGFYPQECVDVYTAVDAYTAGSAYCEFAEDRKGRIRAGYLAD
                     MVILDKDIFEIPVDEIKNVRPVRTIVGGKTVYRS"
     gene            3563..3871
                     /locus_tag="PK898_09170"
     CDS             3563..3871
                     /locus_tag="PK898_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU20242.1"
                     /translation="MPDFGQPFSGLANERKLSEQELIRSIRFMVAAEYEAIQLYMQLA
                     ESTDNKLAIDVLKDIADEERVHAGEFLRLLKELAPDEEDFYQDGAQEVEEMIEKLRKK
                     "
     gene            4085..4348
                     /locus_tag="PK898_09175"
     CDS             4085..4348
                     /locus_tag="PK898_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20243.1"
                     /translation="MAAGKQKFTLEDLIMMLGDQMNAVYRMADEALPLAEQLKLLDML
                     GKTCSRLASLIEQQRKTQGDDSSRLNTVLQEVLSDLRKQGDHE"
     gene            4453..5994
                     /locus_tag="PK898_09180"
     CDS             4453..5994
                     /locus_tag="PK898_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20244.1"
                     /translation="MDEKGSIEISRVLSDVTLFSAVGGGIRLRNYQRNVAEAVVESVI
                     KQQGKSFVVMFPRQSGKNELQAQIETYLLLVFSREGGEIVKISPTRKPQSINAMRRLE
                     RTLRRNIFTKDLWKKESGYSYRVGNAAIFFLSGAPESNIVGATASLLLELDEAQDILP
                     SKYDHEIAPMAASQNATRVFWGTAWTEDSLLSREMQSAGKAERIDPADRRVFRIGADL
                     VTKEVPAYGKFVAEQIRLLGRDHPLVRTQYFSEELNGQSGLFHEKRLEKMRGSHPFLY
                     EPIMGERYILLIDVAGADETSASPDAENESRRDSTALTFCRIRDPLLAEQEVSGGGYW
                     QVVRREIWLNLPLTEQVERICQRMIVWMPEYCVVDASGVGAGIASLLLKRFGTIRILP
                     FVFTAASKSKLGWDFLSIIDTERWQEAKGNSMTDTEQSDLQDHFFRELRFCKSEVLAG
                     AEKKLRWGIPAGTRDPATGEWMHDDLLMSAALAAVIDRSLREMKTETLVVPGRDPLRD
                     LDRGY"
     gene            5996..6577
                     /locus_tag="PK898_09185"
     CDS             5996..6577
                     /locus_tag="PK898_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU20245.1"
                     /translation="MKLNLPFQGNYPITQTFGELYTDPTGHKGIDYALPAGTVVRSAG
                     MGKIIQTAEEAHGYGRFILIDHGEGLQTIYAHLSEIVILVGQSVEAGQIIGFSGNSGN
                     STGPHLHFELRLNGKAVDPQPYLTQDGGDWVVCCEMLNVRTGPGIGYPVCGGLVYGTG
                     VKELSKSETLWVEIAPGQWAAAFFEGEKLMERG"
BASE COUNT         1815 a         1395 c         1878 g         1830 t
ORIGIN      
        1 ttggagggcg acctcctggg agagtaggtt atcgccaaca tgattctttt tgttttaatc
       61 cgataaccat tgttgtcata tcttacagta ttttcggttg acagaaaatt actttcgaat
      121 ataaataaac ctaatcttat ctgactgatg agattgatag aggcgcggaa ttcatcagta
      181 gctgttccgg aatcgctcaa ccgatgatga aggaacaatg aaagggaatt tcgccgaaat
      241 gaaagaaacg gttgagagct ctttcattgg gcataaggaa aatatcttta tgactgtcac
      301 tttttggtgg agcgctatca tgtgtcaatg tttaagcctg caataggatt ttaaataacg
      361 gccggtgcga agcgcttcag ccgttatttt atttccggag gatactgatg gacgagaaac
      421 aaaggctgga agcattgaca tctgaaaaaa ccgaatttcg acgcgaaatc ggcgtatttg
      481 gcggtgtcag cattatcggc ggaatcatga ttggttccgg tattttttac ctcggttcat
      541 atgttttaat acggaccggg atgagtcagg gactctcgct gctggcatgg gtattgggag
      601 gactgatctc tttgttgggc ggtttgtgtt ttgccgaatt gggcgcttcg attccccgtg
      661 ccggcggtat gaccgtctat ctaaacgagg catttcatcc cctcatcgga tttttgaacg
      721 ccttcagcgg cttgctgatt ggaagtccgg gctcgattgc ggcgattgcc attgcgttac
      781 ccaccgcaat gcgaacattt ttcccgatca gtgattcggg tatcaagatc atcgcgattc
      841 ttctgatcgt cggattaacc gtttttaatt attttggaat ccgaccggga agccggttgc
      901 agagcgcttc catgatcgca aaattgattc cgatcggcat aattctcttt gcctcattat
      961 tcttgggaaa agtttctccc gatttaagca tggttcccga agcgggaccg gtcggtttcg
     1021 gtaagctcgt cagcatggtc gcatttgccg tgattgcgac gctgtgggct tatgaaggtt
     1081 ggacaaatct caacaatgtc accgaagaag taaagaaccc gcgccggaat ttgcctctgg
     1141 ctttaatcat cgcaatcggc ggaattacgg ttttatatac cttatttaat tacggaatct
     1201 atcgggtgtt gccgtttgat cagatcaagg cttttatcga atcggggaat tattatctcg
     1261 gtacggaagc ggctaaagca acgttgggaa acgccggagc ggtggtggtg accgtcgcaa
     1321 tggtattgtc catgttcgga tcgctgaatg gctgtatttt ggcttttccc cggacttatt
     1381 atgcgatggc ggaggaagga cactttttcc atgcttttgc cgaacttcat ccgaaatata
     1441 aggtcccggc aacggggttg attgttcaat gcattatttc ggtgattttg gttttggtaa
     1501 gaaatttgga tcagctgacc agcttggtga tatttaccag cgttctctat aatatgctga
     1561 cggtactggc tgttattcgg ttgcggaaaa aatatccgga tattccccgg ccttttaaaa
     1621 tctggggcta tccgtttacg gtaatcctga cggcattgat attcctcggt ttggtaatca
     1681 atacttttat cgaagatccg gtaagtgcga ttacggggat ggtcattccg gtaatcggta
     1741 cggtcgtata cttccttttt gatcgatatc gaaagaaaga agctgccgcg gccgcacaga
     1801 agaattaaaa ggttaaacgg aggaaaaaat gcgtacagca atttataacg gaaaaatcta
     1861 tttagaccgc ggggaattcg ccgaagcggt attgattgaa ggggataccg ttttgcaagt
     1921 cgggacgaca gccgaaattt tagcttcgcc ggacctttcg gaaaaaatcg attgcgaagg
     1981 ccggacggtc attccgggtc tgaacgattc tcatatgcat ctgatgatga tcggagaagc
     2041 gttagagcaa ttatccttag agaactgcaa atcggtcgcg gaattaatca ccgcttccaa
     2101 agcctatctg gctcaacatc cggaagccgc gaagaagggg ttatacggaa gagcctggaa
     2161 tcaggattat tttatcgatg agaagcgaat tcccaaccgt cacgacctgg atcaaatttc
     2221 caccgaaata ccggtggtat tagaacggat atgcgggcat attttagtta caaataccaa
     2281 agcaatcgaa atgttgggtt tgaaagccgg atctcctcaa tataagggag gtgtgtttca
     2341 tcttgaagaa gacggaagtc ctaacggcgt ttttacggaa gatgcctgcc gctatcctct
     2401 ggggttaatt caggaatcga ctcttgaaga gaaaagaatg ttgttgctga aggcaatgga
     2461 atatgccgtt tcccgcggaa tcaccagcgt tcaatcgaac gatctcagtt attatattaa
     2521 agaccataac ggatttatcc gaatgattca cagtctgtat gaagatgaag ttgctccgct
     2581 tcgttatcgt ttgcaggcat gctttcaatc gccggaagct ttcgcggaaa gttttgaaaa
     2641 cgggctgtat tctcatgctt ctgacctcag tgatgattat tttgccatcg gaccgttaaa
     2701 gctgttcaag gacggcagtt taggtgcccg caccgcgatg gtacggcatc cttatttgga
     2761 tgacccggaa aatcccgata acaggggtgt tgagacgact gatgcggcaa caatggatcg
     2821 atattgtgaa attgccgcaa agcacggagt acaggtggtt acgcacgtca tcggtgatcg
     2881 cgcggtcggt gatacgctcg acagttacga aaaagttctg atcgacggtg aaaacaaata
     2941 tcggaacagc ctgattcatt gccaactaac cacgctggaa gatttgaagc gcatcgcaag
     3001 aatggggatc ttagtccaat atcaaccgat atttttgaat tatgatcatc aaatctgtga
     3061 agctcgtgtc gggaaggaac tggcttcgac ttcctatgcg tttcggacac tgcaggattt
     3121 gggagggaaa atctcctacg gaacggattc gccggtagaa gattgtaatc cgtttgaaaa
     3181 tatctattgt gccgtaactc gcaaagggct ggacgggcaa ccgccggaag gattttatcc
     3241 acaagaatgt gtggatgtat atacggctgt cgatgcctat actgccggca gcgcctactg
     3301 tgaatttgct gaggatcgta aagggcggat ccgagccggt tatttggcgg atatggtgat
     3361 ccttgataag gatatttttg aaatcccggt cgacgaaatt aagaatgtcc gtcccgtgcg
     3421 gaccattgtg gggggaaaga cggtttaccg gtcctgatta ttccgaggca agccgttatg
     3481 atttatttgg gcgactgaaa aggcggatta taatttttga caggttttgt aaaattcgtt
     3541 taagaaagag gaggaaaaga aaatgccaga ttttggacaa ccgttttccg ggcttgcaaa
     3601 cgaacgaaag ctcagtgaac aggaattaat ccgttcaatt cgtttcatgg tcgccgccga
     3661 atatgaagca atccagttgt atatgcagct cgccgagtcg accgataaca aacttgccat
     3721 tgatgttttg aaagatattg cggatgaaga aagagtacac gccggtgaat ttctgcgact
     3781 gttgaaagaa ttggcgcccg atgaagaaga tttttatcag gacggagcgc aagaagtcga
     3841 agaaatgatt gagaaattgc gtaaaaagta atttgcagta taaaagagtg catcgcccga
     3901 tgcactcttt ttttatgtat tgattcccgg aagcatgaaa acaaattttc gattgtgatt
     3961 gtccgagttg ttcggacagt ttcctttatc ctccgctttt tggcagttgc ggatggcttc
     4021 ggaatccgtt atcctcagaa atagaattaa cgttctaata tagcgatgag aaaaggattc
     4081 aaaaatggca gccgggaaac aaaagtttac tctcgaagat ttgatcatga tgttgggaga
     4141 tcagatgaat gcggtttacc ggatggcaga tgaagcgttg ccgctggcag aacagttgaa
     4201 actgttggat atgctcggga aaacctgttc ccgattggcg agcttgattg aacaacaacg
     4261 gaaaacgcag ggtgatgatt caagccgttt gaatacggtt ttacaggagg tgctgagcga
     4321 cttaaggaag cagggagatc atgaataaag gattgtctgt ttcgaaaccg ctgaaccaaa
     4381 agtcgaaagc ggataccggt cgaacggctg cgaggatcgt ttcttctcga aaatcggaaa
     4441 ataatatcga acatggatga gaagggatcg atcgaaataa gccgagtgct atccgatgtc
     4501 actttgttca gtgcggtcgg aggtggtatt cgcttaagaa attatcagcg gaatgtggcg
     4561 gaagcggtcg tggagtccgt tatcaagcaa caggggaagt ctttcgtggt gatgttcccg
     4621 cgccagagcg gcaaaaatga gctgcaggca cagatagaaa catatttatt attggttttt
     4681 tctcgggagg gcggagaaat cgttaaaatc agcccgaccc ggaagccgca aagtattaat
     4741 gccatgcgca ggctcgaacg cacgttacgg cggaacatct tcacgaaaga cttatggaag
     4801 aaagaatcgg gctatagtta ccgggtagga aatgccgcga tattcttcct gagcggagca
     4861 cccgagagca atattgtcgg tgcgaccgct tctctgttgc ttgaattgga tgaagctcag
     4921 gatattctcc catccaaata tgaccatgaa atcgcaccga tggcagcctc tcaaaacgcc
     4981 acaagggtat tctggggaac cgcatggacg gaggattcgc ttctttcacg ggaaatgcaa
     5041 tcagccggaa aagcggagcg gatcgacccg gctgatcggc gtgttttccg aatcggagcg
     5101 gatctggtga cgaaagaggt gccggcatac ggaaaatttg tcgctgaaca aattcgtctt
     5161 ctgggaagag atcatccgtt ggtccgcacc caatatttca gtgaggaact gaacgggcaa
     5221 agcgggttat ttcatgaaaa gcgtttggaa aaaatgagag gttctcatcc gtttctatat
     5281 gaaccgatca tgggggagcg atatattctg ttgattgatg tggcgggtgc ggatgaaacc
     5341 agcgcttcgc cggacgccga aaatgaaagc agaagagatt cgaccgcact gactttttgc
     5401 cggattcgag atcctttatt ggcggaacaa gaggtttccg gcggcggata ttggcaggtg
     5461 gtgcgacgtg aaatatggtt gaatttgccc cttacggaac aagtggaacg aatctgccag
     5521 cggatgatcg tttggatgcc ggaatattgt gtggtcgacg ccagcggagt gggcgccgga
     5581 atcgccagtc tattgttgaa acgtttcgga accattcgta ttctgccgtt tgtctttacc
     5641 gccgcctcga aaagcaaatt aggttgggat tttctttcga tcatcgatac cgagcgttgg
     5701 caggaggcga aaggaaattc gatgacggat acggaacaat cagatttgca agaccatttt
     5761 ttccgcgaat tgcgattttg caaatcggaa gtgcttgccg gggcggagaa aaaattgcga
     5821 tggggcatac cggcaggaac acgtgacccg gcaaccggcg aatggatgca tgatgacttg
     5881 ttgatgagcg ccgcattggc agcggtcatc gaccgatctt tacgggaaat gaaaacagag
     5941 acgttagtgg ttccgggacg ggatccgctg cgggatttgg acaggggata ctaatatgaa
     6001 attaaatttg ccgtttcaag gaaattatcc gatcactcag actttcggag agttgtatac
     6061 cgatccgacg ggtcacaaag ggattgatta tgctttaccg gcgggaaccg ttgtgcgatc
     6121 cgccggcatg gggaagatca ttcaaacagc cgaagaggct cacggatatg gtcgttttat
     6181 tttgatcgat cacggagaag ggctgcaaac gatttatgct catttatcgg aaattgtcat
     6241 tctcgtagga caatcggttg aggcggggca gatcatcggg ttcagcggga acagcggaaa
     6301 ttcgacagga ccgcatctgc atttcgagct tcgtctgaat ggaaaagcgg ttgacccgca
     6361 gccttatttg actcaagacg gcggagattg ggtagtttgt tgtgaaatgt tgaatgtgcg
     6421 taccggtccc ggaatcggtt atccggtttg cggaggtttg gtttatggca caggtgttaa
     6481 agagctgtcg aaatcggaga cgttgtgggt ggaaatagct cccggacagt gggcggcggc
     6541 tttttttgag ggtgagaagc tgatggaaag agggtagcag taggcgattt caatccggaa
     6601 tgatgagact gaaaggcgtc ggctttcaag cggctgttgt cgaactcttt ttgccctcac
     6661 cctgctctct cccagcgggc gaggtttttg gtttcggcgt ccctttttaa aaataaataa
     6721 tgtaggggtc ggttccaaac cgacccttct ttgtaaccat gcccgaaaag gcatccgtag
     6781 ggggcatcgt ccttgcgaag caagccgaga atgtgtaggg gacggcgtcc ccgacgtccc
     6841 gccatatacc attgtttaat acaaaactct acacagaaca ttttttgtgg aatttaaaac
     6901 ttttttcacc ctcaccct
//
LOCUS       DAOSMM010000081         6673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_11548_length_6673_cov_14.244334, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000081 DAOSMM010000000
VERSION     DAOSMM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11548_length_6673_cov_14.244334"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /gene="rimO"
                     /locus_tag="PK898_09190"
     CDS             <1..1296
                     /gene="rimO"
                     /locus_tag="PK898_09190"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU20246.1"
                     /translation="NTFHLISLGCAKNTVDSNTVANILQNAGYSFNIDPKQSEYLIVN
                     TCGFIQSARDEAVETLQGLAADKKPGQFLLAIGCMAELYSRQILEACPEVDGVLGTRK
                     ITDILQLITGVKKTNIQLNPVPEEILTSQFTIQGPAAYLKIADGCGRYCSFCSIPLIK
                     GSWISRPQADILADAVRLQEMGIKELILIAQDTTAYGIDRGEKDGLPDLIEKILSAAP
                     DIPRIRVLYAFPGFVSDRLIDLLSNNERVLPYLDIPLQHAHPEVLKRMHRPADMDWVR
                     KTIAKMRSANPNFAIRSTFIVGFPNETEEEFETLYDFLDEMKFDRVGVFSYSFEEGTA
                     SAVYGDTVPEEVKRERLDRLMKLQQGISREINRKFIGRKMDVLVERQQDGLLIGRSYR
                     DAPDIDGLVFVEGKAKIGDIIKVRIHTALEYDLIGKRIG"
     gene            complement(1426..2748)
                     /locus_tag="PK898_09195"
     CDS             complement(1426..2748)
                     /locus_tag="PK898_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20247.1"
                     /translation="MNQNKSNSDKNPKTPKNSENTPNPSSQNYSGSYGSHIPQGEPTP
                     YDPSRSYYPQQQGYYPPPQYPQGQPSMPGNKNPQLQPKKNQKKAAKPKKQGNGKWVWL
                     GILIMLVLIAAGGVIGYQTGIQARQNSYKEQSIKAAAQQYNLAIADIQNGKYENAKTR
                     LEYVLSVDPNYPGATEKYTEVVVALYPKETPTPFYTPTPAPTSTPDTRGEEELLNTIR
                     ANMAAQQWETAIANIEALRNKNLSYKSIEVDGMYYIALRNYGIQLINNGYLEPGIYKI
                     TLAEAFGPIDALASNQRDAARSYLAGAGFWEIDWAKALEYYSNAYLTAPGMYDRASGY
                     TAQERYIQASFEYANRLAQTGDYCASIEYYNQAFSMSANEQYAPTATAAYLECYPPTA
                     TPEVQIQQPTEMVFTPTVPGMSGGESGQVEIPPVDNSTEPIPEPETVP"
     gene            complement(2812..4416)
                     /gene="murJ"
                     /locus_tag="PK898_09200"
     CDS             complement(2812..4416)
                     /gene="murJ"
                     /locus_tag="PK898_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOU20248.1"
                     /translation="MKLSSLSRTSLMIVVFFGIDKVIAIIRQLIIARQFGLSAELDVF
                     NIANNLPDMLFILISGGAMAMVIIPIMTEIIQKESREASWRVFSSIANLAFVVSLILA
                     IFIAIFARPIVTSEIGIAPGFTTEQQEHVVNMMRLNLIATLIFSISGLVMGGLQANRK
                     FFLSALAPILYNIGQIFGAVVLSPQEPYRIGQIELPAFGMGIYGLAGGVILGAILHLL
                     IQIPGLIRLKFKWYPMIQWKDPYVQKTIRIMIPRILTVFFVQLIFIIRDNLASRLETG
                     SVTALSYGYMFQQLPETLIGTALGTAILPSLSLFISENKKAEFQKTFEIACRLSVALT
                     IGIAVIMGVGLGPIVQRVLGFNVAENALLMWTLRGFLVGLTGHCLLEVVNRAFYAQQN
                     ARIPLIGTILNLVVYIILGRILYRPLNAPGISLTDSISFSLQALFLLLLLRMEPAERN
                     NRFYGKKGKQSAPSVPQMNAAPQLWNPIIRSAIGAGIAGIITYEAMHYNPVFSDNLVK
                     SIIGMGAAFLVYLIFILPEIKALRHF"
     gene            4508..5185
                     /gene="rsmI"
                     /locus_tag="PK898_09205"
     CDS             4508..5185
                     /gene="rsmI"
                     /locus_tag="PK898_09205"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU20249.1"
                     /translation="MRKNNALYVVATPIGNDDDITLRAINTLKSADALICEEYRNGNR
                     LLRKLNIENKEIISLNEHNEPAQIENILIRLAQGQSLALVSDCGTPLFSDPGHKLISE
                     VSAAGFRVVPVPGASALTAAISVAPLHLKKFYFAGFLPPASEGRLAELRRIKSRQENI
                     PVILMDTPYRLAAVLKDVCNIFGKEHKITLACDLTTEDERILTGSCAEIMKKIGTKKA
                     EFMLIIH"
     gene            5361..5433
                     /locus_tag="PK898_09210"
     tRNA            5361..5433
                     /locus_tag="PK898_09210"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5395..5397,aa:Glu,seq:ttc)
     gene            5683..6672
                     /locus_tag="PK898_09215"
     CDS             5683..6672
                     /locus_tag="PK898_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU20250.1"
                     /translation="MQRERISENVYWFQSEIYAQVTAGVIIGPQWAVVVDTLALPEEA
                     QEIRKFVTETIGVPIRYLVNTHFHADHAWGNCFFPGATIIAHTLCAKYLKEIGIPALE
                     ETKKQNPSFLNSHIVLPHLTFSHGELILKIGKKNLIIMLTPGHTADGISVFVEEDRVL
                     FAGDAFLPIPQLNEGDYRQSIDVIRKIGDMGLENIIQGHGDIVLRGEIDDAVQENIKY
                     IENIHEIARKATKRRNPAEFLNEYNIEACGKSRVYMGGLAEQLNKRNLIWMFKQVVAE
                     EGLPKSDDEYEDEPEEDDFEDMLPNSDTLPDDYETPGDEDDFYDSDEDFDDDF"
BASE COUNT         1934 a         1427 c         1460 g         1852 t
ORIGIN      
        1 aacacttttc atctaatttc cctcggctgt gcaaaaaaca cggtcgattc caataccgta
       61 gccaatatat tacagaatgc gggctattca tttaatatcg atcccaaaca gtccgaatat
      121 ctgattgtta atacctgcgg gtttattcaa tccgcccgtg acgaagccgt cgaaacgtta
      181 caaggattgg cagccgacaa aaaaccgggg caattcctat tggcgatcgg ttgtatggct
      241 gaactttaca gccggcaaat tttggaagct tgtcccgaag tagatggtgt tctgggaacc
      301 agaaaaatca ctgacattct tcagctgata accggtgtca aaaaaacgaa tattcaactt
      361 aatccggttc ccgaagaaat tttgacgagc caatttacga ttcagggacc cgccgcatat
      421 ctgaaaatcg ccgacggatg cggcaggtat tgctctttct gcagtattcc gttgatcaaa
      481 ggcagttgga tcagccggcc gcaagcagac atcctggctg atgcggttcg acttcaggaa
      541 atgggaataa aagagttgat tttgatcgct caagacacta ccgcttatgg gattgatcgc
      601 ggtgaaaaag atgggctgcc ggatttaatc gaaaagatcc tttcggcagc tccggatatt
      661 ccgcggatcc gtgtccttta tgcattcccg ggattcgttt ccgatcggtt aatcgatctt
      721 ctttcaaaca atgagagggt cttaccttat ttggatatac cgctgcaaca tgcacatccg
      781 gaagtcctca aaagaatgca ccgccctgca gatatggatt gggtcaggaa gaccatcgca
      841 aaaatgcgtt ccgctaaccc gaattttgcc atccgctcaa cctttattgt gggctttccg
      901 aacgaaaccg aagaagagtt cgagacactt tatgactttc tggatgagat gaaattcgat
      961 cgtgtcggag ttttttcgta ttctttcgaa gaagggaccg cgagcgcggt ttacggcgac
     1021 acggttcccg aagaagtcaa acgggaacgg ctcgatcgct tgatgaagct gcaacagggt
     1081 atttcaagag aaatcaatcg gaaatttatc gggagaaaaa tggatgttct cgttgagagg
     1141 caacaagacg gattgctgat cgggcgaagt tatcgtgatg ctccggatat tgacgggttg
     1201 gtttttgttg aagggaaagc aaaaatcggt gatattatca aagtcagaat tcatacggct
     1261 ttggaatatg atttgatcgg gaaacgaatt ggttaaagtt aatagaacat gcgttttccc
     1321 ttcggaaaat cttctatgaa aatcagcata taaaacgaaa aacaaaaatg cctcaagatt
     1381 ttagaggcat ttttgattct ttttcccgaa ttctattaac tcgctttacg gtaccgtttc
     1441 gggttcagga atcggttcgg ttgagttatc aaccggagga atctcgacct gacccgattc
     1501 gccgccggac atccccggaa cagtcggagt gaacaccatt tcggtcggtt gttggatttg
     1561 tacttccggc gtcgcagtcg gaggataaca ttccagatat gcagcagtag ctgtgggcgc
     1621 atattgttca tttgcactca tagaaaatgc ttgattgtag tattcaatcg aagcgcaata
     1681 atcgcctgtt tgagccaatc ggttggcata ttcaaaagaa gcctgaatat aacgttcctg
     1741 tgcggtatat cccgaagcgc gatcatacat tccgggtgcc gtcaaataag cattgctgta
     1801 atactcaagc gcttttgccc aatcgatttc ccagaagccg gcccctgcca aataactgcg
     1861 cgccgcatcc cgttgattgc ttgccaatgc atcaatcggt ccgaaagctt ccgctaaagt
     1921 gatcttataa atcccgggct ccaagtaacc gttattaatc aactggatcc cgtaattcct
     1981 gagagcaata taatacatcc catcgacttc gatagatttg taactcaaat ttttatttcg
     2041 aagagcttca atattcgcta tggcagtctc ccattgttga gcagccatat ttgcccgaat
     2101 cgtgttcagc aattcctctt cccctctggt atccggagta gaagtcgggg cgggagtcgg
     2161 agtataaaaa ggtgtgggag tctctttggg atatagcgca accaccactt cggtgtattt
     2221 ttctgtcgcg ccgggataat tcggatcgac acttagtaca tattccaacc gggttttcgc
     2281 attttcgtat tttccgtttt gaatatccgc gatggctaaa ttatattgct gagccgccgc
     2341 tttaattgat tgctctttat aagaattttg ccgtgcttga attcccgttt gataaccgat
     2401 cacccctcct gcggctatta ataccaacat gatcagaatg ccaagccaaa cccattttcc
     2461 gtttccctgc tttttcggct tcgcagcttt cttttgattt ttcttgggct gtaattgagg
     2521 attcttattc cccggcatcg aaggttgccc ttgaggatat tgcggaggcg gataatatcc
     2581 ttgctgttga ggataataac tacgcgatgg gtcatatgga gtaggctcac cttgcggtat
     2641 atgacttccg tagctaccgc tgtaattttg agaggacgga ttcggcgtat tttccgaatt
     2701 cttcggtgtt tttggattct tatcactgtt tgatttgttt tgattcattt tttactccgg
     2761 cgacagatat cgctatctat ctgcacaaac ttctttcttc gtaaaacttt tctaaaaatg
     2821 acgcaaggct ttaatttcag gcaaaatgaa aatcaaatag acgagaaaag ctgcgcccat
     2881 tccaataata gattttacca gattatcaga aaacaccgga ttgtaatgca tcgcttcata
     2941 ggtaatgatt ccggctattc ccgcaccgat ggcagatcgt atgatcggat tccaaagctg
     3001 cggagctgca ttcatttgag gaacacttgg cgcgctttgt tttccttttt tcccataaaa
     3061 ccgattattt ctctcagcgg gttccatccg taacagaagc agaagaaaaa gagcttgcaa
     3121 tgaaaacgag atggaatccg tcaaactgat cccgggagca ttcaaagggc gatacaggat
     3181 cctgcctaag atgatatata ctacaaggtt caaaatcgtt ccgatcaaag ggattcgtgc
     3241 attttgttgg gcgtagaaag cccgattgac cacttcaagc aaacaatggc cggtcagtcc
     3301 caccaaaaat ccccgcaaag tccacatcag cagagcattt tccgcaacgt taaatcctaa
     3361 aacccgttga acgatcggac ctaatccgac gcccataatg actgctatac cgattgtgag
     3421 cgcaacggat aagcggcagg caatttcaaa agtcttctga aattccgctt ttttattttc
     3481 cgaaataaat aaggaaagcg acggtaagat tgcggttccc aaagccgtcc cgatcagtgt
     3541 ctccggtaat tgttgaaaca tgtagccgta gcttaaagcc gttaccgatc cggtttccaa
     3601 ccgagatgcc aagttatccc gaataataaa aatcaattgg acgaaaaaaa cggttaagat
     3661 cctcgggatc atgatccgaa tggttttttg aacatacgga tctttccatt gaatcatggg
     3721 ataccattta aattttaatc gaatcagacc ggggatttga atcagtaagt gaaggatagc
     3781 cccgaggata accccgccgg ctaagccgta gattcccatt ccaaaagcgg gaagctcgat
     3841 ttgtccgatc ctgtatggtt cttgaggaga aagaaccacc gcgccaaata tttgaccgat
     3901 attatagagt atgggtgcta acgcagaaag gaaaaatttt cgattcgctt gcagcccgcc
     3961 cataaccaat ccgctgatcg agaaaatcag tgtcgcaatt aaattcaggc gcatcatatt
     4021 taccacatgt tcttgttgtt cggttgtaaa acccggagcg attcctatct ccgaagtgac
     4081 gataggacgt gcaaatatcg cgataaatat cgctaaaatc aatgaaacca cgaaggcaag
     4141 attggcgatg gatgaaaaga ctctccaaga cgcttcccga ctttccttct gaataatttc
     4201 tgtcataatc ggaataatca ccattgccat ggcgcctccg ctgatcagaa taaataacat
     4261 atcggggaga ttatttgcaa tgttaaaaac gtccaattcc gcagaaagtc caaattggcg
     4321 ggcgataatt aattgccgga taatggctat gaccttatcg atcccgaaaa agacaacaat
     4381 catcaaggaa gttcttgaaa gggaagataa cttcatagtt ccattctatc attcaagatt
     4441 tatgacagca tgacaaaata tgttccggat caaaatttct cccaaaaaat cctgatagaa
     4501 ttcagctatg agaaaaaata acgcccttta tgttgtggca acgcccatcg ggaatgatga
     4561 tgacatcact ttgcgagcaa ttaacactct caaatcggcc gatgcattga tatgcgaaga
     4621 atatcgaaac ggaaaccggt tgttgcgaaa gctgaacatt gaaaacaaag aaatcatttc
     4681 attaaatgag cataacgaac cggcacaaat cgaaaatatt ctcattcgtt tagcgcaggg
     4741 acaaagcctt gcacttgttt ccgattgcgg aacaccttta ttttccgatc ccgggcataa
     4801 attgatttcc gaggtttcag cagccggttt tcgtgtggtt cccgttccgg gagcatctgc
     4861 tctgacagca gctatttccg ttgcgccgct tcatttgaag aaattctatt ttgccggatt
     4921 tcttccgccc gcttccgaag gaagacttgc cgaacttcgc agaattaaat cgagacaaga
     4981 aaatattccg gtcattttga tggatacgcc ttatcgcttg gcagccgtat taaaagatgt
     5041 ttgtaatatc tttgggaaag aacataaaat tacgcttgct tgtgatttaa ccaccgaaga
     5101 tgagcggatt ttaaccggat cgtgtgcaga gatcatgaaa aaaatcggga caaagaaagc
     5161 cgaatttatg ctgataattc attaaagaag tcgaaatata ggtctttgtt cgaaaatccg
     5221 caaatttgat cgaattctat caaaaactca tttttcttac ccgtttttca ggatgacaat
     5281 ttggagaata acaacacaag tttcagactt atcttgacgg atagaaaaac aactgctaaa
     5341 atagcaacgt tattcaacaa gcctccatcg tctaggggac caggacgcaa ccctttcaag
     5401 gttaaaacgt gagttcgaat ctcactggag gtgctgaaaa gatatccgag tcggatatct
     5461 ttttttatcc gtacggataa taatgatcat atttctatca aaatttaata tttccctgcg
     5521 gaaaaatgat acactcaata tccattaata ttgagggaat atagcatttt caagacaaaa
     5581 atcagtgata ttttgacttt tgaaattgaa tatgctattc tatcagaata tctgagcgat
     5641 ttatttttcc gctcattgac tgtcgaaggg gactgtaaaa ttatgcagcg tgaacgtatt
     5701 tctgaaaatg tttattggtt ccaaagtgaa atctatgccc aggttaccgc aggtgtcatt
     5761 ataggacctc aatgggctgt tgtggtcgac actctggcac ttccggaaga agcacaggaa
     5821 atcaggaaat ttgtcaccga gactatcgga gttccgataa gatatttggt caatactcat
     5881 tttcacgcgg atcacgcttg gggaaattgt tttttcccgg gagcgacgat tatcgcacat
     5941 acgctgtgtg ccaagtactt aaaagaaatc ggaattccgg ctttggaaga aacaaaaaaa
     6001 cagaacccgt cttttctgaa ttcacatatc gttctccctc atttgacctt ctctcacggt
     6061 gaattaatct tgaaaatcgg aaagaaaaat ctgattatta tgttaactcc cggacacacc
     6121 gcggacggca tttcggtctt cgtcgaagaa gatcgtgtcc tttttgcggg agacgccttc
     6181 ctgccaatcc cgcaactgaa tgaaggcgat tatcgtcagt ccattgatgt tatccgtaaa
     6241 atcggcgata tgggtttgga gaatattatc caggggcacg gggatatcgt tttacggggc
     6301 gagattgatg acgctgttca agaaaatatc aaatatattg aaaatattca tgaaattgcc
     6361 agaaaagcca caaaacgccg aaacccggca gaatttttaa atgaatataa tatcgaagct
     6421 tgcggaaaga gccgggtata tatgggcggg ttagccgaac aattgaataa acgaaatctt
     6481 atttggatgt ttaaacaagt tgtggctgag gaaggccttc ccaaatcgga tgacgaatat
     6541 gaagacgaac cggaagaaga tgatttcgag gatatgctgc ccaattcgga tactctgccc
     6601 gatgattacg agacccccgg tgatgaggat gacttttatg actccgatga agactttgat
     6661 gatgattttt aat
//
LOCUS       DAOSMM010000082         6663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_11579_length_6663_cov_19.503329, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000082 DAOSMM010000000
VERSION     DAOSMM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_6663_cov_19.503329"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /gene="tatA"
                     /locus_tag="PK898_09220"
     CDS             <1..208
                     /gene="tatA"
                     /locus_tag="PK898_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOU20251.1"
                     /translation="MWRPGFAEILIVLVIVILLFGPGRIAKVAKEIGASITAFKEGLN
                     EDSDKKKTSEETQSDDENAKSNHP"
     gene            234..614
                     /locus_tag="PK898_09225"
     CDS             234..614
                     /locus_tag="PK898_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20252.1"
                     /translation="MGFFNIGIPELILVLTIMLVFLGPDKLRESSRNFARFIRKVVRS
                     ETWRNFSVMYHEMKHYPEEIMKEVNLEETMKELNEINARTQAELDRIQNSVRSMPKAV
                     DSDSSESNPLDSDKENTPETKDSL"
     gene            611..1336
                     /gene="tatC"
                     /locus_tag="PK898_09230"
     CDS             611..1336
                     /gene="tatC"
                     /locus_tag="PK898_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOU20253.1"
                     /translation="MNQEESSTDFKEFLEGMSEHLTELRNRLIVIVLSLCFAIIIAFF
                     FSDSIMAFLARPIGGLEKLQSIEITENFTAVFRVALLAGLVLDSPIIFYEILAFILPA
                     LRSNEKRILFTFLPLLILFFIAGILFAFFILLPAAIPFLTSFTDIPTTIRTNSYFSFT
                     TNMLFWIGIVFEMPIFIYFLTKVKIVNSGLLLKNWRQAVVICAILAMLITPTVDPVNM
                     TILLIPLVLLYFLSILFAKIAER"
     gene            1468..2211
                     /locus_tag="PK898_09235"
     CDS             1468..2211
                     /locus_tag="PK898_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOU20254.1"
                     /translation="MKEINQQNKTKFIRGTLENCDQAGDLAVVIDVLRAFTTAAYAFS
                     KGAKTIYCVAEVEKALELKSCIPGSLTMGEVNGLSIPEFDFNNSPTALMQHDIQEKIL
                     IQRTTAGTQGLIRSSHAKTLVAASLVTAKATAEFIRLRSPEEIFFVETGITSDGRGDE
                     DTACADYIEGILTGNPIPEQQIIERVRNSVNGRRFIQNASPDLPAEDLEFVCDINRFH
                     WIIQAERVDNQIVRLSKKEKFQWNFKNPS"
     gene            2187..3242
                     /locus_tag="PK898_09240"
     CDS             2187..3242
                     /locus_tag="PK898_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU20255.1"
                     /translation="MEFQESVLITKKVRGEDFHSLEPDYLIFEPKETNAEFTREELDE
                     ILPKVTAIVAITDVNAEMIAKALSLKVIVANGAGFDNIDITAATALRIPVINVPDATA
                     YSTAELAMALILDVSRRVSELDRLLRRNADNVSDFFSIGKNPGQNLAGKTLGIIGLGH
                     IGMTLAEMCWPFRLNIQYYNRHRLPLSQEKGIRYVPFEELLETSDIISIHCPLNNSTR
                     NMINYEAFSRMKQGAIFINTARGPIADYDALIYFLENGKLKGAGLDVYPAEPEIPVKL
                     FKLENVVLTPHIGTNTVETRQGMADAICDVIRTVCEAEPGQIFKNVINREIYQREPVM
                     SQPDESSEIHPEKESES"
     gene            3239..5251
                     /locus_tag="PK898_09245"
     CDS             3239..5251
                     /locus_tag="PK898_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20256.1"
                     /translation="MSLKKSFFLFLFLISLISQIHPAFAESEPINSDDVTLIGINDAI
                     YSLTEAKTAEITTADQLHATLEVNDVIVEKDRVLVRIIIWGLPVTWGDKVIDQNRIYG
                     SYLPVAELGIPSGKWLTPSSGSRYSLLSNGNTLVIAGLLEFLTDEQPAVVSFNFNQIP
                     FDMEILSEGSTMILELEKGDHQERSERSPLSDQKDGVVFSLMNTAQTTKTTMIQPGFA
                     TIREDESISKIGWIKVERSDGKKYLLTRTDPYGFNLSDDSKTILVDSYTFMPLSSSDP
                     LKIGLDYVYVKRSGNHEVELSFPAPLDFTSEKAALNVEMPLDEFSGKINGYQVFNKTI
                     QGEEMTILRLFMNNDPHISSISFKDSLAEDDFTYSNCGYLPDSDQFACDVQITKLGQS
                     DLTLYFDSFEYRIDGNWEIKWTPVSIAFASNPEPVPGTRLEIDYSKLGSSSSNEIHEL
                     TDLLRSFSDSLTANPGWILQKAQTQAVAAGGEYPDLVQHGDEVLKNNRTVSESWDQID
                     SDGYVINNISITRDTKGNILTGTWNRSDCQILLPQGYKVPYESGPSDTIYPFLYGNEF
                     FTLLNTSAEFIGRKDCEIDGQAGWCYTFEHSLSSSPDSGQPTFMNRYTFLIDPETGEI
                     LREETECQLGGPGTPLQPCIQTKTVEISYRKSLDSELQTMIDSIIF"
     gene            5264..6586
                     /locus_tag="PK898_09250"
     CDS             5264..6586
                     /locus_tag="PK898_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOU20257.1"
                     /translation="MTASAKFTFPEKFLWGTATSAHQVEGENNNNNWSLWEAQPGRIR
                     NGDVSGKACDWWKGRRWQEDFLRAADTGQNTHRMSIEWSRIQPKENLWDESAIDYYRE
                     MIRGMISRGLRPLITLHHFTDPIWFYEMGGWQNEKAPEYFLRFAEKIIPAFKPLVKDW
                     ITINEPNVYILCGFISGHFPPGNINNFSAANIVLRNMIKAHAAVYHLIHEQQPEASVG
                     IAIHFRDLFPKTNLPPDRWMTRLAHHLFNNSFTDTISSGKFHYGPYFIHIPQAKNTQD
                     FLGLNFYSGNQVRFTFNPNNFLSYPEGASLSDTGFIANEPESFSRALKWAAGYGKPIL
                     ITENGTESDEDTFRRDYLAAHIHRLWRAWSNGIPVKGYYHWSLIDNFEWERGWSQRFG
                     LWRLNVETQVRTKRLSADFYERICRKNSLDSEDMETFAPRIYREIFPV"
BASE COUNT         1926 a         1427 c         1445 g         1865 t
ORIGIN      
        1 tatgtggaga cccggattcg ctgagattct gattgtcctt gtcatcgtga tcttgctatt
       61 tggaccaggg cgaatagcaa aggtcgccaa ggaaatcgga gcaagcatta ccgctttcaa
      121 agaaggattg aacgaagatt ccgataaaaa aaagacttcg gaagaaacgc aatccgatga
      181 tgaaaatgca aaatcaaatc atccttaatc tcgttcgtta ttaattattc agaatgggct
      241 tttttaatat cggtattccg gaactgattc tggtactgac aattatgttg gtcttcttag
      301 gacccgacaa attacgggaa agttcccgta atttcgctcg gtttattcgt aaggtggttc
      361 gttccgaaac atggcgcaat ttttccgtga tgtatcatga aatgaaacac tatccggaag
      421 aaattatgaa agaagtaaac cttgaagaaa cgatgaagga attaaatgaa ataaatgccc
      481 ggacacaggc ggaattggac cggattcaaa acagcgttcg ttcaatgccg aaggcagtcg
      541 attcagattc atccgaatcc aacccgttgg attcggataa ggagaacact ccggaaacaa
      601 aggattcttt atgaatcaag aggaatcctc taccgacttc aaagaatttt tagaagggat
      661 gtcggaacat ttaaccgagt tacgaaatcg gctgattgtt attgttttat cattatgttt
      721 cgcaattatt attgctttct tcttttctga ttcgattatg gcatttcttg cccgaccgat
      781 cggcggactg gaaaaactgc aatccatcga aatcacggag aatttcacag cagtcttccg
      841 agtcgcattg cttgccggat tggttctgga cagtcccatt attttttacg aaattttagc
      901 cttcatttta cctgctttaa ggagcaatga aaaaaggatc ttgtttacat ttttgccgct
      961 tttaatcctc tttttcatcg cgggaattct ttttgcattc tttattcttt tgccggcagc
     1021 aattcctttc ttaacttctt ttaccgatat cccgacgaca atccgtacga acagctattt
     1081 ttcatttacg acaaatatgt tattctggat cggaattgtc tttgaaatgc cgatttttat
     1141 ctactttttg acaaaagtga aaattgtaaa ttccggattg ttattaaaaa attggcggca
     1201 agccgtcgtc atttgtgcca ttcttgccat gctcatcact ccaacggtgg atcccgtcaa
     1261 tatgacgatc ctcttaatcc ctttggtttt actttatttt ctcagtattt tgtttgcaaa
     1321 aattgcggaa cgctgattcg atcttcctca gggaatactc tcggcttaaa aaaatccggt
     1381 tacataaatt cgaaaacttc ccgaaatttt gcaatgggtt taaaagaaag ggatctttta
     1441 ttataattat tttcgggtga aatatatttg aaagaaatta atcagcaaaa caagacaaaa
     1501 tttatccgcg gaaccttgga aaactgtgat caagccggag atttagcagt ggtgatcgat
     1561 gtactgcgcg ctttcacaac cgcagcatat gctttttcaa agggagcaaa aacaatttat
     1621 tgtgtcgccg aagttgaaaa agcacttgaa ttaaaatcat gcattcccgg cagtctgacg
     1681 atgggagaag taaacggtct cagcattccg gagttcgatt tcaataattc accgactgct
     1741 ttgatgcagc atgacattca agaaaaaata ctcattcaaa ggacgactgc cggaacacag
     1801 ggactgatac gttcttctca tgccaaaacg ctggtcgccg caagcctcgt aacggcaaaa
     1861 gcaaccgcag aatttatccg tttgcggtca ccggaagaga tcttttttgt tgagacggga
     1921 attacttctg acggaagagg agacgaagac actgcctgcg cagactatat cgaagggatt
     1981 cttaccggta accccattcc ggaacagcag ataatcgaac gggttcgaaa ctctgtcaac
     2041 ggccgacgtt ttattcaaaa tgcctcaccc gatttaccgg ccgaggattt ggaatttgtt
     2101 tgtgatataa atcgttttca ttggatcata caagctgaaa gggtggacaa tcaaatcgtc
     2161 cgattatcaa aaaaggagaa atttcaatgg aatttcaaga atccgtcttg atcacaaaaa
     2221 aagtccgggg ggaagatttt cattctctgg aaccggatta tctgatcttt gaaccgaagg
     2281 aaacgaatgc cgaattcacg cgcgaagaat tggatgaaat tctgccaaaa gtgactgcga
     2341 ttgtggcgat caccgatgtc aacgccgaaa tgatcgccaa agctctttca ctgaaagtca
     2401 tcgtcgccaa cggagccggt tttgataata tcgatataac cgcagccacc gctcttcgaa
     2461 tccctgtgat caatgtcccg gatgccaccg cttattccac cgccgagctg gcaatggctt
     2521 taatcctgga tgtcagccgt cgggtttccg aattggatcg gttgctgaga agaaacgcgg
     2581 ataacgtctc ggactttttt tcgatcggca agaatccggg gcaaaattta gccgggaaaa
     2641 cattgggaat tatcggcttg ggtcatatcg ggatgacttt agctgaaatg tgttggccat
     2701 ttcgattaaa tattcaatat tacaaccgcc accggttgcc gttatctcaa gaaaaaggca
     2761 ttcgatatgt tccttttgaa gaattgctgg aaacttccga catcatttcg atccattgtc
     2821 cgttgaataa ctccacccgc aacatgatta attacgaggc attttcaagg atgaaacagg
     2881 gtgccatctt tatcaatacc gctcgcggac cgattgcgga ctatgacgcg ttaatctatt
     2941 ttctcgaaaa cgggaaattg aaaggtgccg gattggatgt ttatccggcg gaacccgaaa
     3001 ttcccgtcaa gctattcaaa ttggaaaacg tggtccttac tccgcatatc ggtaccaata
     3061 ccgtcgaaac aagacaggga atggcggatg ccatatgcga cgttatccgt acggtctgcg
     3121 aagcggaacc gggacaaatt ttcaaaaatg ttattaatcg agaaatctac caaagagaac
     3181 cggttatgtc gcaacccgat gaaagttctg agattcatcc cgaaaaggaa tctgaaagtt
     3241 gagtttaaaa aagtctttct ttttattttt atttttgatc agccttatca gccaaattca
     3301 tcccgcgttc gccgaatccg aaccgattaa ttccgatgac gtcacattaa tcgggatcaa
     3361 tgatgcaata tattcgctga cggaagcaaa aaccgcggaa attactactg ctgatcaact
     3421 ccatgccaca ctcgaagtca atgatgtcat cgttgaaaag gaccgggttt tggtgcgaat
     3481 cataatttgg gggcttccgg tcacatgggg tgataaagtc atcgatcaaa accgaattta
     3541 cggcagttat ttacccgtcg ccgaattggg aattccgtcg ggaaaatggc tcactccctc
     3601 ttcgggctct cgttacagtc tgttatcaaa cggaaatacg ctggttatcg ccggattatt
     3661 ggaattttta accgatgaac aacctgccgt tgtttccttc aatttcaatc agattccctt
     3721 cgatatggaa attctttccg aaggatcgac gatgatattg gaattggaaa aaggtgatca
     3781 ccaagaaaga agcgaacgtt cgcccctgag cgaccaaaaa gacggggttg ttttttcttt
     3841 gatgaatacc gctcagacca ccaaaaccac tatgattcaa ccggggttcg cgacaattcg
     3901 tgaagatgaa tccatttcaa agatcggttg gataaaggtc gaacgatcag acggaaaaaa
     3961 atatttatta accaggaccg atccgtatgg atttaatctc agcgatgatt caaaaacaat
     4021 tctggtggat tcttatacct ttatgcctct ttcaagttcc gacccgctga aaatcggctt
     4081 ggattatgtt tatgtcaaac gctcgggaaa tcatgaagtc gaattaagct ttccggctcc
     4141 tctcgatttc acctcagaga aagcggcatt gaatgttgaa atgccattgg atgaattctc
     4201 cggcaaaatc aatggatatc aagtttttaa caaaaccatc caaggcgaag aaatgaccat
     4261 cctcagactt ttcatgaaca atgatccgca tatatcctct atttctttta aagacagtct
     4321 cgccgaagat gattttacct attcaaattg cggttatctg cccgacagcg accaatttgc
     4381 ctgtgacgtt caaatcacga aattgggaca aagtgatttg acattgtatt tcgattcgtt
     4441 cgaatatcgg atagacggaa actgggagat taaatggaca ccggtatcca tcgcttttgc
     4501 gtccaacccc gagcctgtcc cggggacgcg gctggaaatc gattattcca aattgggctc
     4561 ttcttcttca aatgaaattc acgaattaac cgatttgctc cggtctttct ctgattctct
     4621 caccgcaaat cccggctgga tcctgcaaaa ggctcaaaca caggcagtcg cagccggcgg
     4681 agaatatccg gatttggttc agcatggtga tgaggttctg aaaaataacc gaaccgtttc
     4741 ggaatcctgg gatcaaattg attcagacgg atatgtgatc aataatattt caataaccag
     4801 agatacaaaa ggcaatattc tcaccggaac ttggaatcga tcggattgtc agattctcct
     4861 tccccaaggg tacaaagtcc cttatgaatc ggggccttcc gatacgattt atcctttttt
     4921 atacggtaat gaattcttta cgctgctgaa tacatcggct gaatttatcg gtcggaaaga
     4981 ttgtgaaata gacggacaag ccggttggtg ttataccttt gagcattctt taagctcctc
     5041 acccgattcc ggtcaaccta cttttatgaa tcgctatact ttcctgattg atccagaaac
     5101 aggggaaatc ctgcgggaag aaacggaatg ccagcttgga ggaccgggaa ctcctttgca
     5161 accttgcatc caaacaaaaa cggtcgaaat cagttaccgg aaatctttag attcagaact
     5221 tcaaacaatg atcgattcaa taatttttta aaaggagaga ttcgtgacag cttcggcaaa
     5281 gtttactttc cccgaaaaat ttttatgggg aacagctacc tctgcgcatc aagttgaagg
     5341 tgaaaacaat aacaataatt ggagtctttg ggaagcccag cccggccgta tccgaaacgg
     5401 agatgtgagc ggtaaagctt gtgactggtg gaaagggagg cgttggcagg aagactttct
     5461 tcgggcagcc gatacgggac aaaataccca tcggatgtcc atcgaatggt ctcgaattca
     5521 gcctaaagag aatttgtggg atgaatcggc aatcgattat tatcgtgaaa tgattcgcgg
     5581 catgatcagc cgcggattac gtcctttgat aacattgcat catttcaccg atccgatttg
     5641 gttttatgag atgggcggat ggcaaaatga gaaagcaccg gaatatttcc tgcgttttgc
     5701 cgaaaaaatc attccggcat ttaaaccgct tgtcaaggat tggataacga tcaacgaacc
     5761 gaacgtttat atattatgcg gttttatctc cggacatttt ccgcccggaa atattaacaa
     5821 tttttcggcg gcaaatattg tccttcgcaa tatgatcaaa gcccatgcgg cggtatatca
     5881 cctgattcat gagcaacaac ccgaagcgtc cgtcggcatc gcaattcatt tccgtgattt
     5941 gttccccaaa acaaatttac cgccggatcg ttggatgaca aggctcgcac atcatctctt
     6001 caataactca tttaccgata cgatatcaag cggaaaattt cattacggac cgtactttat
     6061 tcatataccg caggcaaaga acactcagga ttttttgggc ctgaattttt actccgggaa
     6121 tcaagtccga ttcactttta acccgaacaa ctttttatcc tacccggagg gagcatcctt
     6181 gagtgacacg gggttcatcg caaatgagcc ggaatctttt tcaagggcac tgaagtgggc
     6241 agccggctat ggcaagccga tcctcatcac cgaaaacgga accgaaagtg atgaggatac
     6301 gttcagacgg gattatttag ctgctcatat tcatcggctt tggcgcgcat ggtcaaacgg
     6361 aataccggta aaaggatatt atcattggtc actcatagat aatttcgagt gggaacgcgg
     6421 ctggagtcaa cgattcggac tttggcgttt gaacgttgaa acacaagttc gcaccaaacg
     6481 tctttcagct gatttttatg aacggatttg ccgtaagaat tctttggatt ccgaagatat
     6541 ggaaactttt gccccgcgaa tttatcggga gatctttccc gtttagagtc cagaccgaac
     6601 cggttctttt tgacagaagg caagaaatcg ttcgttatct gattctcaga tcaatattta
     6661 ttt
//
LOCUS       DAOSMM010000083         6506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_11943_length_6506_cov_17.334677, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000083 DAOSMM010000000
VERSION     DAOSMM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11943_length_6506_cov_17.334677"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PK898_09255"
     CDS             <1..1197
                     /locus_tag="PK898_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOU20258.1"
                     /translation="MADLFAGQEMTVLDLLIHHCGLNSESAGTLWYGSDYSREEVVRR
                     VRYLRPVTGFRSTYAYQNVCYLIAGLIIEKICGMSWDDFICKYIFSPLEMERSFPNLK
                     ALQLSGMDNVATPHTQLNGKLSVIPYRNHDNVGPAASVHSTALDLAHYLQMFLNHGVY
                     RGKVILQPESVENLHSMHIVVPPAPAKDLIHPRLLTDFSGYGLGWFIQDYQGKKRVGH
                     SGGVDGMRGRMEIFPALNRGIVILTNSEDRRAYQSILYSAFDMFLESEPIDWINVWEN
                     DPEMKQTVTEPERIPNTSPSLPDAAYTGVYEDSAYGEIIVRKEAHRLILQFSHTPAFT
                     ADLEHWHFDTFRLIWHDPYIPRGLVTFSLNCEGKVESMHIDQPRLLDVDFNELDYAIR
                     KKPDRD"
     gene            complement(1226..1432)
                     /locus_tag="PK898_09260"
     CDS             complement(1226..1432)
                     /locus_tag="PK898_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOU20259.1"
                     /translation="MADWIVGFIVVLLIGWAIYRIIQRRKNPLGNCGGSHSIYGIPDS
                     EIPSYCSNCRARELCRRQAPKARK"
     gene            complement(1439..3553)
                     /gene="feoB"
                     /locus_tag="PK898_09265"
     CDS             complement(1439..3553)
                     /gene="feoB"
                     /locus_tag="PK898_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOU20260.1"
                     /translation="MIDSHIDSEQDEFIPKADHTHVGTIADLKSDNNPNGLRLTFALA
                     GNQNSGKTTLFNYLTGSNQHVGNWPGVTVEKKTGKVKKYPGITLVDLPGIYSLSPYTD
                     EEMITRNYLLDEKPDAIINIVDANNLERNLYLTLQLAELGIPMVIALNMMDEIAANGD
                     VIDVDGLERDLGIPVVPIVARNGSGVEDLIRRAIGVAMHREQPPIRDICEGEVHKAIH
                     SIGHIIEPKARARGYSARFVSTKLIEGEDPIKQAIGLTPEEEKIIDEIISEMEKATGL
                     DREAAIADSRYRFITKVCSENLKRRRKIGEQSTSDKIDKVLTGKYSALPIFLAIMLFV
                     FWLTFGPIGSRLSEGFSELIDTGIGIVDHALTYAGVSEWLQSLVVDGILGGVGSVLNF
                     LPVILILFICLSVLEDCGYMARAAFIMDRLLRKIGLSGRSFIPFMMGFGCTVPALMAT
                     RTLENKRIRYLTVLLTPFMSCGAKVPVYTLFIAAFFAKGKILVIFSLYLIGILVAVLM
                     GLILKSTLFRGTPAPFIIELPPYRWPAPHNVAIQVWEKIKDFLERAVTIILLATVVIW
                     FLGSFDPKFYWTADSSQSILAMLGGLFAPLFLPLGFGNWQAITSLVSGFMAKESIIST
                     MAVVYKAADQGLQTVLQSIFTPASAFSFLIFVLLYPPCIASVSTMNKEVGTKWTGFAI
                     VLELATAWIVSFLAYHIALLIL"
     gene            complement(3546..3800)
                     /locus_tag="PK898_09270"
     CDS             complement(3546..3800)
                     /locus_tag="PK898_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOU20261.1"
                     /translation="MIKTLDQFVPGDQGEVVKVGGQGAIRRRLLDMGITPGTRILLIK
                     TAPMGDPLEITLRGFNLSIRKEDARYIELKMDSPEEESND"
     gene            4111..4776
                     /locus_tag="PK898_09275"
     CDS             4111..4776
                     /locus_tag="PK898_09275"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF005302.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HOU20262.1"
                     /translation="MLLEEIRSEIIEYGKKLIDNRLTKGTGGNLSACDRKTGLMAITP
                     SGIDYYQIKPEQIMLIDIQSGEIKDGTAIPSSEVDMHRILYKYREDLNAVIHTHSTFA
                     AALSCLNITLPPVHYLVAYAGVDVRCAKYATYGTVALAKNAFEAMKDRKACLLANHGL
                     LAGGPDLANAFNITEEIEFCCELYYRAKSIGEPVILSKEEMVRMVDRFKNYGKRIEEH
                     EQI"
     gene            complement(4858..6360)
                     /locus_tag="PK898_09280"
     CDS             complement(4858..6360)
                     /locus_tag="PK898_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20263.1"
                     /translation="MDNENKSNIENEKWNEEPKDSFVVTHHSILCGGKKISYTATAGR
                     MILKEEIVQKEGDQKNQYEGEKPRAEIFFVAYTLDGVDDPTKRPITFAFNGGPGSASI
                     WIHMGLLGPKRVLLNDDGTPLPPPARLTENEYSVLDMTDLVFIDPIGTGFSRAVKGDK
                     PNDYWGWTKDVESVGDFIRLYVSRNQRWGSPKFIAGESYGTTRTTGLSDYLSDKYGMY
                     LNGIVLVSTALDFATLDFNDGNDLPYILYLPSYAATAWYHKKLKAKYQEMPLEKYLEE
                     VRHFAGGEYLQALFNGSRMAEKQIEQVADKTANYIGLTREYVLRANLRVTLPRFSKEL
                     LRSERRTVGRYDGRFIGIDRDAAGEEPEDDPAGYEINGTFAGAFNDYICRELGYKTDL
                     PYAFSTDLWKTWSYKEYENKYVQLEEVLRKTMVRNKFMKVWVLNGYYDMATPFYASEY
                     VFSHLQLDPVLQPNLSLTYYEAGHMMYIHKPSLEKFRKDAEFFFENALKT"
BASE COUNT         1814 a         1530 c         1375 g         1787 t
ORIGIN      
        1 atggctgatc tttttgccgg gcaggaaatg accgtccttg acttgttaat ccatcattgc
       61 ggcttgaaca gcgaaagtgc cggtacactt tggtacggtt ccgattattc tcgagaagaa
      121 gtagtccgtc gtgttcgtta cctcagaccc gtcaccggtt ttcgcagtac gtatgcctat
      181 caaaacgtgt gttatttgat cgccggattg attatcgaaa aaatctgcgg aatgagttgg
      241 gatgatttta tttgtaaata tattttttca ccgcttgaaa tggaacgctc ttttcccaat
      301 ctgaaagcct tgcaactgtc cggaatggac aatgttgcca caccccatac gcaattgaat
      361 ggcaagttga gcgtgattcc ttatcgaaat catgacaatg tcggtccggc agcttccgtt
      421 cattcgacag ccctcgattt agcccattat cttcaaatgt tcctgaacca tggcgtctat
      481 cgcggaaaag tgattttaca gcccgaatcc gtcgaaaatc tgcattcgat gcacattgta
      541 gttccgccgg ctcccgctaa ggatttgatt catccccgtc tgcttaccga tttttcggga
      601 tacggattgg gttggtttat tcaggattat cagggcaaaa aaagggtcgg tcattccggc
      661 ggagtagacg gtatgcgcgg acgaatggaa atttttccgg cattaaaccg gggcatcgtc
      721 atccttacca attctgaaga tcgaagagcc tatcaaagta ttctgtattc ggcttttgat
      781 atgtttctgg aatcggagcc gatcgattgg atcaatgttt gggaaaacga cccggaaatg
      841 aaacaaacag taacggaacc ggaacggatt ccgaacactt ctccttcctt acctgatgcc
      901 gcctataccg gtgtttatga agattccgca tatggtgaaa taatcgtcag aaaagaagcc
      961 cataggctga tcctacaatt cagtcataca cccgctttta ccgcagatct tgaacattgg
     1021 cattttgata ccttccgctt gatttggcat gatccatata ttccccgagg attggtcact
     1081 ttttcactga actgtgaagg aaaagtcgaa tcgatgcaca tcgatcaacc gcgattgctc
     1141 gatgtcgatt ttaacgagct ggattatgcc attcgaaaga aaccagaccg agactgacga
     1201 cgatcaacca aatacaatgc gacaattatt tgcgtgcttt cggtgcctgt ctgcggcaca
     1261 actcacgagc ccggcaattg ctgcaatacg acggaatttc ggaatccgga attccgtaaa
     1321 tcgaatggct tccgccgcag ttcccgagcg ggtttttcct tctttgaatg atacggtaaa
     1381 ttgcccaacc aatgagcaaa acaacgataa atccgacaat ccaatcagcc atagaatctc
     1441 ataaaatcaa taaagcaata tgatacgcca ggaaagatac gatccaagcg gttgccaatt
     1501 cgagaacgat cgcaaacccg gtccactttg ttcccacttc cttattcatg gtcgagacgg
     1561 aagcgataca gggcggataa agaagcacga aaatcaggaa actgaatgcg gatgccggag
     1621 taaatatcga ttgaagaacc gtttgcagtc cctgatcggc cgctttataa accaccgcca
     1681 ttgtactgat gatgctttcc ttcgccataa atccggatac caaggaagtg attgcctgcc
     1741 aatttccaaa acctaagggc aggaagaggg gcgcaaaaag tccgccgagc atcgccaaaa
     1801 tgctctgtga agaatccgcg gtccaataga atttcggatc aaatgagccg aggaaccaga
     1861 tgactacggt agccaacaga ataatggtta ccgctctttc gaggaaatcc tttatttttt
     1921 cccaaacctg aattgccaca ttatgcggcg ccggccagcg ataaggagga agttcgataa
     1981 taaaaggagc cggcgttccg cgaaacaaag tgctttttaa gatcaagccc atcagaacag
     2041 cgacaagaat cccgatcaga tagagtgaaa aaataacaag tatcttccct ttggcaaaaa
     2101 aagctgcgat aaacagcgta taaaccggta cttttgcgcc acacgacata aagggtgtca
     2161 acagaaccgt caaataacga atccgtttat tttccagcgt ccgtgtcgcc atcaaagccg
     2221 gtacggtaca tccgaatccc atcataaacg gaataaacga acggccggac aagccgattt
     2281 ttcgcagcaa tcgatccatt atgaatgccg cccgagccat atacccgcaa tcttccaaaa
     2341 cggataaaca gatgaaaagg atcaggataa ccggtaagaa attcaacaca cttccgactc
     2401 cgcccaatat tccgtcgaca accaggctct gtaaccattc cgacacacct gcatacgtca
     2461 gagcatgatc gacaatgccg attccggtat cgattaactc ggaaaaccct tcacttaatc
     2521 ggctgccgat cggtccaaac gtcagccaaa aaacaaacaa cattatcgcc aaaaaaatgg
     2581 gaagagctga atatttgcct gttaatactt tatcgatttt atccgaagtc gattgttcgc
     2641 cgatttttct ccttcgtttg agattttcgg aacagacttt ggtgataaac cggtagcggg
     2701 aatccgcgat cgccgcttcc cggtcaagtc cggtagcttt ttccatctcc gatataattt
     2761 catcgatgat tttttcttct tcgggagtca ggccgatggc ttgcttgatc gggtcttcac
     2821 cttcaatcaa cttggtggaa acaaagcgtg ctgaatatcc tcgcgctctc gcttttggct
     2881 cgataatatg accgatcgaa tggatcgctt tatggacttc cccttcacaa atatcgcgaa
     2941 tcgggggctg ctcgcgatgc atggcgacac cgatggcacg gcggatcaaa tcttccactc
     3001 cgctgccgtt tcttgccaca atcggtacaa ccggaattcc gaggtcacgc tccaagccat
     3061 ccacatcgat cacatcaccg ttggcagcga tttcatccat catattcaat gcgatgacca
     3121 tcggaattcc gagctccgcc aactgaagcg tcaaatacaa attgcgttcc aggttattcg
     3181 catcaacgat attgataatc gcatccggct tttcatcgag aaggtaattt cgggtgatca
     3241 tctcctcatc tgtataagga gaaagcgaat aaattccggg cagatccacc aatgtgatcc
     3301 ccggatattt tttcactttt ccggtttttt tctcgacggt cacaccgggc caatttccga
     3361 catgttggtt ggagccggtt aaataattga aaagggttgt tttaccgctg ttttggtttc
     3421 ccgccagtgc aaaagtcagg cggagaccgt tggggttgtt atcgctttta agatcggcga
     3481 tcgtgcccac atgagtatga tcagcctttg gaataaactc gtcttgttcg gagtcaatat
     3541 gcgaatcaat cattcgactc ctcctcgggt gaatccattt tcaactcgat ataacgagca
     3601 tcttcttttc gaatactcag attaaatccg cgtaaggtga tttcgagggg atcgcccatc
     3661 ggggcggttt tgatcaggag gattcgggtt ccgggtgtga tacccatatc gagtaacctt
     3721 cgccgaattg ctccctgccc accgactttg acgacctcac cttggtcgcc gggaacaaac
     3781 tgatctaatg ttttaatcat ctctctgcct ttcggaacat cttcaagttc ctgcttgcag
     3841 ggaatattct atcacttgcg acgaaagcgg aaagaaccaa ttgggtccga ttattaccgg
     3901 atgaatactt ccgcaaagag agtcaaaatt ctgactgcgg caaattcgtc gaaaccgcta
     3961 ctttcccgtt ccataaaaac tgattttttc ctttatacgg aatagaaaat cataatcatc
     4021 ggtcagtgcg gattatctcg gttataaaat aagaaataaa gatttttccg gaacaagtca
     4081 aacatcccgg aagatagctc aggaggttat atgctgttgg aagaaatccg atcggaaatt
     4141 atcgaatatg ggaaaaaatt aatcgacaat cgtttaacca aaggaaccgg cggaaacttg
     4201 agtgcctgcg accgaaaaac cgggttgatg gcaatcaccc ccagtggaat cgattactat
     4261 caaatcaaac cggaacaaat tatgctcatc gatattcaat cgggagaaat taaggatgga
     4321 actgccatcc cgtccagcga agtggatatg caccgaattc tatataaata tcgggaggat
     4381 ttaaatgccg taatccatac tcacagcact tttgctgcgg cgctctcttg tctcaatata
     4441 accttacctc ctgttcacta tctggtcgct tatgcgggag ttgatgttcg atgtgcaaaa
     4501 tatgcgactt acggaaccgt tgcgcttgca aagaatgcct ttgaggcgat gaaagatcgg
     4561 aaagcctgcc ttttggcaaa ccatgggctg ttagccggcg gtccggattt ggcgaatgcc
     4621 tttaatatca ccgaagagat cgagttttgc tgcgaactat attatcgggc aaaatcgatc
     4681 ggtgaacccg taattctttc caaagaggaa atggtccgaa tggtagatcg gtttaagaat
     4741 tacggtaagc ggattgaaga acatgaacaa atttagcctc gtttttttct gaaaaaatta
     4801 aataaaaaag aggccgatat tatttttcgg tctctttata aataatttaa tcgattttca
     4861 agttttcaaa gcattttcaa agaaaaattc tgcgtcttta cggaattttt ccaacgacgg
     4921 cttatggata tacatcatat gccccgcttc ataataagtg agactcaggt ttggctgcaa
     4981 aacaggatca agctgtaaat ggctgaacac atactccgaa gcatagaatg gtgttgccat
     5041 gtcataatat ccgttcagaa cccagacttt cataaactta tttcgaacca ttgtttttct
     5101 caaaacttct tccaattgaa catatttatt ttcatattct ttatagctcc acgtcttcca
     5161 taaatcggtc gaaaatgcat agggcagatc ggtcttataa cctaattccc gacagatata
     5221 atcattgaat gctcccgcaa aagttccgtt tatctcataa ccggccggat catcttccgg
     5281 ttcttcgccg gcagcgtcac gatcgattcc tataaatctt ccatcataac ggccgacagt
     5341 tcggcgttcg ctccttaata attctttcga aaatcgcggc aatgtcaccc gtaaatttgc
     5401 ccgcaataca tattcgcgtg tcagcccgat ataattcgcc gttttatcgg caacttgttc
     5461 gatttgtttt tccgccatgc ggctgccgtt aaacaaagcc tgaagatatt cgcctccggc
     5521 aaaatggcgc acttcttcca gatatttttc caaaggcatt tcttgatatt ttgctttcag
     5581 ttttttatga taccaagcag ttgcagcata agaaggcagg taaaggatgt aaggtaaatc
     5641 atttccatcg ttgaaatcga gcgtggcaaa atccagtgcg gtcgaaacca gtacaatccc
     5701 attcagatac atcccgtatt tgtctgaaag ataatcggat aaaccggttg tacgagtcgt
     5761 cccgtacgat tcaccggcaa taaatttcgg cgaaccccat cgttgatttc gagagacata
     5821 aagtcggata aaatcgccga ctgattcgac atctttcgtc catccccagt aatcgttggg
     5881 tttgtcaccc tttacggcgc gcgaaaaacc ggtaccgatc ggatcgataa aaaccaagtc
     5941 ggtcatatcc aaaacagaat attcgttttc tgtcagccga gccggcggag gtaacggagt
     6001 gccgtcatcg ttcaggagga ctcgtttcgg tcccaacaaa cccatatgga tccatatcga
     6061 tgcggatccc ggaccgccat taaaagcaaa ggtaatcggg cgttttgtcg gatcatcaac
     6121 tccgtctaac gtatatgcga cgaaaaatat ctccgctcgg ggcttttctc cttcatattg
     6181 atttttttga tcgccttctt tttggactat ttcttctttc aaaatcattc ttccggcggt
     6241 ggcagtatat gaaatctttt tgcccccgca cagaatcgaa tgatgagtga cgacgaaaga
     6301 atctttgggc tcttcattcc atttttcatt ttcgatgttc gatttatttt cattatccat
     6361 tttttgttcc tcagtttttt tgctattatc tcccgatttc tcatttcaat gccattaact
     6421 atataacaaa aaaagtcgaa aaaaaagagt atttatctgt ttttttgatt ccaaaaattt
     6481 ttgaaaattc ggagcctgaa aataag
//
LOCUS       DAOSMM010000084         6373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_12301_length_6373_cov_18.509022, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000084 DAOSMM010000000
VERSION     DAOSMM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12301_length_6373_cov_18.509022"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..3403)
                     /locus_tag="PK898_09285"
     CDS             complement(80..3403)
                     /locus_tag="PK898_09285"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOU20264.1"
                     /translation="MNHIKNQVEIWEEEVSIPTYEVGIPEKNPMFFENRVYQGSSGKV
                     YPFPIIESISNKKIDKKYQAVFLENDYLFVMILPELGGRIQRALDKSNNYDFVYYNEV
                     IKPALVGLTGPWISGGIEFNWPQHHRPTTFLPVLYRLQENSDGSKTVQMSDMDRMYGT
                     KEVTNITLYPDKAYIEIKGQLYNPTSSPQTFLWWANPAVPANEYTQSIFPPDVTAVMD
                     HGKRDVSKFPIADGIYYKYDYSKGVDISYYKNIPVPTSYMAYRSDYDFVGCYDHQREA
                     GILHIADHHISPGKKQWTWGCGDFGKAWERNLTDHNGAYIELMAGVYTDNQPDFTWLK
                     PFEEKRFTQYFLPYKKVGQVKNATKDVLLSADMRGNHLEFSIYGTHLLEDIRVFISGN
                     NHTVFDQSFTVSPTQIISESVLLPKDSRQNLKLSIFDREGEVLLTYSLDRAKKNKLPS
                     PATPAPNPKDIATNEELFLHGIHLEQYRHPTFDPDPYYLEGLKRDPGDSRINNAYGML
                     ILRRGLIEESEQYFRNAIKRITMRNPNPYDGEPYYNLGYCLFLQGRFQEAFDAFYKAT
                     WSESEQEKSFYYLATIKTIQRDYDQALDFIENSLLKNLHNLKARGLKTILLRKMGHSD
                     EAKIWAEETNTIDPFDLLALNERKLLSDDTVRLPFTIRPENFNFVIEAASDYASYGCY
                     KEAIDLLDRLEPKGPMIHYSLAYYYFRMGDLAESQKQLKSASESDPSYCFPNRIEDIA
                     VLNFAITQNPDDSYAYYYLGNLYYDKKQYQKALTDWKKSTELNDRFATSWRNLAIILW
                     NKEKKPQEAIAAMEKAFSLDPNDARILFESDQLYKKANYPIHDRLENLEKHQSLLEKR
                     DDLYTEYITLLNLSGQYEKALEKISGHIFHPWEGGEGKITSQYKIAHLEMAKKALVEK
                     NSKEAERLLLEALTYPLNLGEGKLIGTKDNDIYYFLGCCYEQACDEDKAVKAWIHACK
                     GSEEISGEMYYYDQPADMILYQGLALFKLTHYERSDLKFHKLIYYGEQHMEDTVTMDY
                     FAVSLPDMQLLEEDLSLRNRAHCHYLIGLGNLGLRNWEKASDHFKETLKINNSHVGAN
                     IHLALSNTAKSNQFFEKKW"
     gene            3612..4487
                     /locus_tag="PK898_09290"
     CDS             3612..4487
                     /locus_tag="PK898_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOU20265.1"
                     /translation="MKVYQEGVLPESDIYFHTCSSQAKELFFYLLCTGKYFCNERYFV
                     DRQAYDSFLILYVKKGSGYVQNGNSPLLPLDEGSVAFINCYIPHKYFTKIGWTIYWIH
                     FDGLLAPKYFNLITEKNLILDPQNPHIIERYLQKIFNTFHEHKRVNEAIISKNIIDLL
                     TELILVATDSESLDNPPRMIEEMLTYISENSSQKLSLPELAKQASLSPYYFSRLFKKE
                     TGYSPFEYVIQVRIDQAKFLLKTTSMPIKELAFQCGFNSVCSFCTSFKKKTGYTPTEY
                     RIKELPNGKKMPSLM"
     gene            complement(4523..5503)
                     /locus_tag="PK898_09295"
     CDS             complement(4523..5503)
                     /locus_tag="PK898_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU20266.1"
                     /translation="MKKVCIFVLLLTMVFGFVMSVSAEDNKLVVYGIYKAGDQTWFID
                     EGAAAKKAVEAAGGEFIFVDAKMSPEEYLKAIDNAIANKATGIVTCIPDQTLSQAVVD
                     KLQEAKIPVVAADDALQDADGKKLVPWVGINAYVIGEANGEWLANYAKENNLVTDPEV
                     GLLILTMDTVSSCVPRAEGEYNKFTELLPEFDKSKIFKSDYDGTTEKGNIAASAIFTA
                     HPEIKKWLVTGANEEGTIGAVRALESIGLDKDACVVGLGGYLAKDEFKKEGGSAMKAA
                     SYFSSDAVGTTSVKILLDIIAGKEVPMETAVDAIMVTPENYVEVMGDAAK"
     gene            complement(5562..>6373)
                     /locus_tag="PK898_09300"
     CDS             complement(5562..>6373)
                     /locus_tag="PK898_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20267.1"
                     /translation="TYLIIAGQNDLSPGSLAAFSGVLCAYLVKIAIPLGIAILITIAA
                     AAVVGFFNAWMVNKIKLEAFIATLVSQSIIRGFAYILCDGKPIAISNANFIKIGKMRF
                     LGVPFSVWVMIVSIVIFGFILSKTKFGRSVYAIGGNKDAARLAGLNPQRIVTISFVMM
                     GIFCGIGGIIFAARMNSGQPAANVNLEFDSITAVILGGVSFTGGVGNMGGTILGIILI
                     QAFNTGLIMVNVPTFWQYVARGALLLFALTSDYIRKERHEKELLEASMRNI"
BASE COUNT         1777 a         1410 c         1256 g         1930 t
ORIGIN      
        1 cctacggttg actgtgcatt gaagataaag agcgggatgt caaggacgct atcccaacaa
       61 atattccctc tcccgtttct taccactttt tttcaaaaaa ttggtttgac ttcgctgtgt
      121 tcgataatgc cagatggatg ttcgctccga cgtgactgtt atttatcttt agagtttcct
      181 tgaaatgatc cgaggctttc tcccaattcc ttaatcctaa atttcccaga ccgatcagat
      241 aatgacaatg ggcacgattc cgcagcgaca gatcttcctc caacaattgc atatccggta
      301 acgaaaccgc gaaataatcc atcgttacgg tatcttccat gtgttgttca ccgtaataaa
      361 ttaacttgtg aaattttagg tcacttcttt cataatgcgt taatttgaaa agtgccaaac
      421 cctgataaag gatcatatct gccggttggt cataataata catctctccg gaaatctctt
      481 cggaaccttt acatgcatgt atccatgctt tcacggcttt gtcttcgtca caagcttgtt
      541 cgtaacagca tcccaagaaa taatagatat cgttatcctt tgtgccgatt aattttccct
      601 ctcctaaatt cagaggataa gttaaagcct ccagtaaaag tcgctccgct tctttcgaat
      661 ttttttctac caaggctttt tttgccattt ccaaatgagc gattttatat tgagaagtaa
      721 ttttcccttc accgccttcc caggggtgaa agatatggcc ggatatcttt tctaatgctt
      781 tttcatattg tcccgaaaga tttaacagtg taatatattc ggtataaagg tcatcccgct
      841 tctcaagcag cgattgatgt ttttcgaggt tttccagccg atcgtgaatc ggataattcg
      901 cttttttgta cagctgatcc gattcgaaca ggattctggc atcattcgga tcgagagaaa
      961 atgctttttc catggcagcg atggcttcct gcggcttttt ctctttattc caaagaataa
     1021 ttgccagatt ccgccatgaa gtcgcgaagc gatcattcaa ttccgtcgac tttttccaat
     1081 cggttaaagc tttttgatat tgttttttat cgtaatacaa atttccgaga taataatagg
     1141 cataactgtc atccggattt tgtgtaatcg caaaattcaa aacagcgata tcttcaattc
     1201 ggttcggaaa acaatagctc gggtcgcttt ccgaggcgga ttttaactgt ttctgtgatt
     1261 cggcaagatc tcccatccgg aaataataat aagccagcga ataatggatc atcggacctt
     1321 tcggttcgag gcgatccaac aaatcgatcg cctctttata acatccgtag gatgcatagt
     1381 ccgatgccgc ttcaatgaca aaattgaaat tctccggacg gatcgtgaaa ggaagtcgaa
     1441 ctgtgtcgtc gctcaaaagt ttccgttcat tcaaggctaa cagatcaaag gggtcgatgg
     1501 tatttgtttc ctcagcccag atctttgctt catcgctgtg ccccattttt ctgaggagaa
     1561 tcgtttttaa accgcgcgct ttcagattat gaagattttt caagagggaa ttctcgataa
     1621 aatccaatgc ttgatcgtaa tctctctgaa ttgtcttgat ggttgcaaga tagtaaaatg
     1681 atttttcctg ctcactttcc gaccaagtgg ctttgtagaa agcatcaaag gcttcttgga
     1741 aacggccttg tagaaaaaga caataaccga ggttataata cggttccccg tcatatgggt
     1801 taggattacg catcgtgatc cgtttaatcg cattccgaaa atattgttcg ctttcttcga
     1861 ttaatccgcg acgcagaatc aacatcccat aggcattatt gatgcgactg tcccctggat
     1921 ctctttttaa cccttccaaa taatacggat ccggatcaaa ggttggatgg cggtattgtt
     1981 ccaaatgaat cccatgcagg aataattcct catttgtcgc aatatctttc ggattcggtg
     2041 caggagtcgc gggcgacggc agcttattct tttttgcccg atcgagagaa taggtcaata
     2101 aaacttcccc ttcccgatcg aagattgaca atttgagatt ttgccggctg tctttcggta
     2161 agagaacgga ttcgcttatg atttgggtag gagaaaccgt aaacgattga tcaaaaacgg
     2221 tgtgattatt tccggaaata aaaacgcgaa tatcttccag caaatgagtt ccatagatcg
     2281 agaattctaa atgatttcct ctcatatccg ccgacaacag aacatctttc gtcgcatttt
     2341 tgacttgtcc gactttttta taggggagaa aatattgcgt gaaccgtttc tcttcaaagg
     2401 gtttcagcca ggtgaaatcg ggctgattat ccgtatatac gcctgccatt aactcaatat
     2461 aagcgccgtt atgatcggtc aaatttcttt cccaagcttt tccgaaatca ccgcagcccc
     2521 atgtccattg ttttttcccc ggagaaatat gatgatcggc gatgtgtaat atccccgctt
     2581 cccgctgatg atcatagcaa cctacaaaat cgtaatccga tcgataagcc atataagagg
     2641 tcgggaccgg aatattcttg taataagaaa tgtcaacccc cttgctatag tcatatttat
     2701 agtaaattcc gtcggctatg gggaattttg aaacatctcg tttaccgtga tccatcacag
     2761 cggtgacatc cggcggaaaa atagattgag tatattcatt tgccggaaca gccggatttg
     2821 cccaccacag aaaagtctgc ggcgaagagg tcggattata caactgtcct tttatttcaa
     2881 tatatgcttt atccggatac agcgtaatat tcgtcacttc ttttgtgccg tacatacggt
     2941 ccatatcgct catttggact gttttacttc cgtccgaatt ctcttgcagc cgatacaaaa
     3001 caggtaaaaa agtggtcggc cggtgatgtt gaggccaatt gaattcgatt ccaccgctga
     3061 tccaaggtcc ggtaagtccg accaacgccg gtttaatcac ttcgttatag taaacaaaat
     3121 cataattatt ggatttatcg agcgctcgtt ggattcttcc tcccaattcg gggagaatca
     3181 tgacgaacag atagtcattt tcaaggaaaa ccgcttgata tttcttatcg atttttttat
     3241 ttgaaatact ttcgataatc gggaacggat agacttttcc gctgctccct tgatataccc
     3301 gattttcaaa aaacatggga tttttttccg gaatgccaac ttcgtaagtt ggtattgaaa
     3361 cttcctcttc ccaaatttct acttgattct tgatatgatt catgattgcc tatcctttga
     3421 aaaaataaac tatctgaata tatcttagca tgcagtttgg ttcaaacaag ataatatctt
     3481 gctattctgg ttaaatatat tgcctttgtt aaaatgaaaa atttggtcaa tttgaccgat
     3541 tttccgaaat attttagaat acttcaaaag agcgagtaaa aaaaattgct gtttttggaa
     3601 aggcagaaaa gatgaaagtt tatcaggaag gggtattacc ggaatcggat atttatttcc
     3661 atacctgcag ttcgcaagcg aaagaattat tcttttattt actctgtacc ggcaaatatt
     3721 tttgtaatga acgctatttt gttgacagac aagcttatga cagctttctg attttgtatg
     3781 tcaaaaaagg ttccggatat gttcagaacg ggaattcacc cttgttaccc cttgatgaag
     3841 gatccgtagc ttttatcaat tgttatatcc cgcataaata ctttaccaag atcggatgga
     3901 caatttattg gattcatttt gacggattat tggcgccgaa gtattttaac ttgatcaccg
     3961 agaagaattt gattcttgat ccgcaaaatc cgcatatcat cgaacgctac ctgcaaaaaa
     4021 tatttaatac ctttcatgaa cataaaagag tcaacgaagc gattatttca aaaaatatca
     4081 ttgacctgtt gaccgaattg attctggtgg ctaccgatag cgaatctttg gacaatcctc
     4141 cccgtatgat cgaggaaatg ttgacttata tttcagaaaa ttcaagtcag aagctttcct
     4201 tacccgaatt ggcgaaacaa gcttctttga gcccgtatta cttcagccgg ctttttaaga
     4261 aagaaacggg atattctcct tttgaatatg tcattcaagt ccgaattgat caagcaaaat
     4321 ttctgctgaa aaccacttcc atgcctatta aagagttggc ttttcaatgc ggattcaata
     4381 gtgtctgcag tttttgcact tcttttaaga aaaaaaccgg atatacaccg accgaatatc
     4441 ggataaaaga attgccgaac ggaaaaaaga tgccttcctt gatgtaggaa ggcatctttt
     4501 ccattaatca aacaactgat tattattttg cggcatcgcc cataacttca acgtaattct
     4561 caggggtgac cattatggca tcaaccgctg tttccatggg gacttctttg ccggcgatga
     4621 tatccagcaa aatttttacg gaagttgtac cgactgcatc ggatgagaag taagaggcag
     4681 ccttcatagc ggaaccgcct tcctttttga attcatcttt tgccaaatat ccgccgagac
     4741 cgaccacaca ggcatcttta tctaatccga tgctctccaa agcgcggacg gctccgatcg
     4801 taccttcttc attggcaccg gtgacaagcc attttttaat ttccggatga gcggtaaata
     4861 ttgcggaagc agcgatgttt cctttttccg tcgttccgtc ataatcggac ttaaaaattt
     4921 tggatttatc gaattcgggg agcagttccg tgaatttgtt atattcgcct tccgcacgag
     4981 gaacgcaact ggaaacggta tccatggtca ggattaataa gccgacttcc ggatcggtta
     5041 ccaaattatt ttcctttgca taattggcaa gccattcgcc gtttgcttca ccgatgacat
     5101 aagcattgat tccgacccac gggaccagtt tcttgccatc cgcatcctgt aacgcatcgt
     5161 ccgccgcaac aacggggatc tttgcttcct ggagtttatc caccacagcc tgtgacagtg
     5221 tctggtcagg aatgcaggta acgatgccgg tggctttatt ggcgatcgcg ttatcaattg
     5281 ccttgaggta ttcttcgggg ctcatctttg catcgacaaa aatgaattca ccgcctgcgg
     5341 cttcaacagc ttttttagcg gctgcgcctt catcgatgaa ccaggtctga tcacctgctt
     5401 tgtaaatgcc gtagacaact aatttattgt cttcggcgga gacagacatt acaaagccaa
     5461 ataccattgt taaaagtaaa acaaaaatac aaaccttctt catttcagta tcctcctgaa
     5521 aaaatttaac aatacctttc ttttcgtatt gttattgacc atcaaatatt tctcatgctg
     5581 gcttccagca actctttttc atgccgttct ttccggatat agtcggaagt cagcgcaaat
     5641 aagagcaaag ctccgcgggc gacatattgc cagaaagtcg gcacatttac catgatcaag
     5701 ccggtattga aggcttgaat cagaataatt cccagaatgg tccctcccat atttccgacg
     5761 ccgccggtaa aggaaactcc tcccaaaatc actgcggtaa tcgaatcgaa ttccaggttt
     5821 acattcgcgg ccggctgtcc ggaattcatt cttgccgcaa agatgatgcc cccgatgccg
     5881 cagaaaattc ccatcattac aaaggatatc gtcacaattc tttgcggatt caaaccggct
     5941 aaccttgcgg catctttgtt cccgccgatc gcataaacgc ttcgaccgaa tttggttttc
     6001 gataagatga atccgaaaat gacgattgag acgatcatta cccagacgga aaacgggacg
     6061 cctaaaaagc gcatcttccc gatcttaata aagtttgcgt tactgattgc tatcggtttc
     6121 ccatcgcaaa gaatataagc aaagccacga atgatggatt ggctgaccaa ggttgcgatg
     6181 aacgcttcca atttgatttt atttaccatc caggcattga aaaaaccgac cacggcggca
     6241 gcggcaatcg ttataagaat cgctatgcct aacggaatcg cgattttcac caaataggca
     6301 cataagactc cggaaaaagc agccaaagag ccgggcgaca aatcattttg tccggcaata
     6361 attaaataag tat
//
LOCUS       DAOSMM010000085         5833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_13846_length_5833_cov_8.555902, whole genome shotgun sequence.
ACCESSION   DAOSMM010000085 DAOSMM010000000
VERSION     DAOSMM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13846_length_5833_cov_8.555902"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..2583
                     /locus_tag="PK898_09305"
     CDS             178..2583
                     /locus_tag="PK898_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20268.1"
                     /translation="MNKKIAPWVLAFLSVILLSLPVIAQPESSTPFVFGSPNFSQKFS
                     PFYADTGYDMDVIEMTFSPLMTTDRVGGIIFNGINGETRPYNGKDYTYYGIADLSVNF
                     DKDADITTYTAKLRNDLKFSDGQPVTADDIIFTYYVYLDPAYVGSTTLNSYKIIGLQD
                     YLTQTTSEIYQKYAKIAEAIQAAGSDHIWAEGDEWTKDQQEAYWNLVKEGWMNEVQKI
                     IDYAYNNFAADYAQEILGKTPEEVGSDEGLKTAFGMAMWGFGDIADGILTTKSGAEFD
                     LNAGNKPTIDDFYKETFAAYAGDADAFFSVEATDVKAKSIVATAKDTFISEEGSKEPE
                     MAAGIPNISGIKKLDDYTVEVKTYGYEAPAIYSILGIYVAPLHYYGDPAQYDYENNRF
                     GHPYGDLSIIEAKTSNPMGSGPYKFIKYDNRIVYFEANENFYLGKPKTQFVQFKETAD
                     NEMVEGVSTGTIDAASISGSKSTFESIAEHNSNKEITGDKITTFKVDNLGYGYIGINA
                     DTVKVGDDPASTESKNLRKALATVIAVYRDVAYDSYYGDAATVINYPISNTSWAAPQP
                     TDPDYKVAFSEDVNGNPIYTSGMTPEEKYEAALEAAKGFLKAAGYTYDEATGKFTAAP
                     EGASLTYEIIIPAGGTGDHPSFALVTDAANALAKIGITLKINDPADTNVLWDTLDAGK
                     QNLWCAAWQATIDPDMYQVYHSSNIVGKGGSDSNHYHIADPELDKAIIEARQSDDQAY
                     RKEIYKTALDIIVDWAVEIPAYQRQNSTIFSTERVKIDTVTPDITTFYDWNAEIQTLE
                     MAQ"
     gene            2741..3727
                     /locus_tag="PK898_09310"
     CDS             2741..3727
                     /locus_tag="PK898_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20269.1"
                     /translation="MFKYFLRRLTYAVFILFFGSLIVYTVIRFLPTSYVENIARQRSN
                     LPGAKSYSQILANLNSVYGLDTDIFTGFLRWGKNAIHGDFGYSWQYNIPVVQKFKEVI
                     WYSVFLNVVTLLVETFISIPLGILAARKQYSKTDYAVTVLSMICISLPTFFLATLLKY
                     IFSVKLGWFDLYGIVGRYYEQLDAWGKFLDMAKHLVLPVLTLSMLNIGGLMRYTRTNM
                     LEVLNTDYIRTARAKGLSEDVVVKKHAFRNTLVPLVSYFSYLIPNMFSGSFITETLFQ
                     IPGIGYISYYAVIHGDIPFAMFYSVLIIFLTQISLLIADFMYAVVDPRVRIN"
     gene            3740..5503
                     /locus_tag="PK898_09315"
     CDS             3740..5503
                     /locus_tag="PK898_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20270.1"
                     /translation="MNQNQTGNRQTNGPQSEEIRLDDEKRIKVLSPTMLVFKRFLRNK
                     LAIVGLVILILMFLFSFLGGVLSPYQQQQVFRKYEVIPKEYASATINQNLRYIVQDGE
                     IFPNSAIAKFVLALNEGQTRFNSEETEYSYKKEGDDFYLISENKKIADVIILAGKGRF
                     TLSQGIDFPEESQKQAIEAIQAGKNEFESNGIKYFLSRKGKITTISMPKEIAIATKLI
                     FDAFENDAIPSLNRIEFVYQAQNALINNIERFSVNGENYRIEISEDSSIIYKDYTNGE
                     RPFASISNFIVQPKSPDVFLTVDFKKKFNEAILSNQNSFSVLSDSGEEIPYTINRVNE
                     TYTIYTQTKAQLIDMYSPPSKEHWLGTDANGMDVLTRLMYGGRISLMVGFVVVFLELF
                     IGVIVGGIAGYFGNTVDTLLMRFVDLFNSIPFWPMVIIAGSVMDTLEVKPTPRIFALM
                     GILGILSWTGIARVVRGQILSLREQDFMVATEAAGIRVSRRIFKHLVPNVMPLLIVQA
                     TLSLGSIIITEATLSFLGLGVKYPIASWGSIINAATNIHIMTNYWFIWIPAGILIVLT
                     VLGFNFVGDGLRDAYDPKMKR"
     gene            5509..>5833
                     /locus_tag="PK898_09320"
     CDS             5509..>5833
                     /locus_tag="PK898_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU20271.1"
                     /translation="MKLFSEKPLKKESGNYISARESYNISKENKRITNAIERERNRKN
                     VPESEYLTVMHNPENVVEYDNLHTYFFTDIGTVKAVDGVSFEVPKGKTVGIVGESGCG
                     KSITSL"
BASE COUNT         1782 a         1144 c         1241 g         1666 t
ORIGIN      
        1 gatgaggatg acttttatga ctccgatgaa gactttgatg atgattttta ataaaatacc
       61 ttagtgtttc cctatagtac aattatttat aatcaaactg tttaaagaaa aacaattttg
      121 agtcttcggg aaataacttt ctaaaacagt ttaacaattt catatcagga ggattttatg
      181 aacaaaaaaa ttgccccgtg ggttcttgca ttcctttctg tgattctgct tagcttgccg
      241 gtaatcgctc agcctgaatc ctcaactccg tttgtttttg gctctccgaa cttcagtcaa
      301 aaattcagtc ctttttacgc tgatacgggt tatgacatgg atgtcatcga aatgacattt
      361 tcaccgctga tgacaactga cagagtggga ggaatcattt tcaacggcat taatggcgaa
      421 accagaccgt ataacggaaa agactatacc tattatggaa tcgccgatct ttcggtaaat
      481 tttgataaag atgccgatat aacgacctat actgcgaaat tacggaatga tctaaaattt
      541 tcagacggtc aaccagtaac agctgatgat attattttca cctattatgt ctacctggat
      601 cccgcctatg tcggttcaac gactttaaat tcttataaga ttatcggact tcaggactac
      661 ctgacccaaa cgaccagcga aatctatcag aaatatgcaa aaatcgccga agccattcaa
      721 gctgccggct ctgatcatat ttgggccgaa ggggatgaat ggaccaaaga tcagcaagaa
      781 gcttattgga atttggtcaa agaaggctgg atgaatgaag tacaaaaaat tatcgattat
      841 gcttataaca attttgcggc tgactatgca caagagatct taggtaaaac acctgaggaa
      901 gtcggcagtg atgaagggtt aaaaaccgct ttcggtatgg caatgtgggg attcggcgat
      961 attgcagatg gcattttaac aacgaaatcc ggtgcggaat ttgacttaaa tgcggggaat
     1021 aaaccgacaa tcgatgattt ctacaaggaa acctttgcag cctatgcggg agatgccgat
     1081 gcattcttct ctgttgaagc gaccgatgta aaagccaaat cgattgtggc aaccgctaaa
     1141 gatacattca tttcggaaga aggttcaaaa gaaccggaaa tggctgcggg gattccgaac
     1201 atctccggaa ttaagaagct ggacgattat acggtcgaag taaaaaccta cggatatgaa
     1261 gccccggcta tttattccat tttgggaatt tatgtcgctc cgttacatta ttacggagat
     1321 ccggcacaat atgattatga aaataatcgc ttcggacacc cttatgggga cttaagtatc
     1381 atcgaagcaa agacctctaa tccgatgggt tcgggaccgt acaagtttat caaatatgat
     1441 aatcgaatcg tatatttcga agccaacgaa aatttttatc ttggaaaacc gaaaacacaa
     1501 ttcgttcaat tcaaagaaac cgccgataat gagatggtcg aaggcgtcag cacgggcaca
     1561 attgatgccg cctcgatctc gggatcaaaa tccacatttg aatcaatcgc cgaacataac
     1621 agcaataaag agattacggg agacaaaatc acgactttca aagttgacaa tctcggctat
     1681 ggctatatcg gaatcaatgc ggataccgta aaagtcgggg atgacccggc ttcaaccgaa
     1741 tcaaagaatt tacggaaagc actggcaacg gtaattgccg tatatcgtga tgtagcttat
     1801 gacagttatt atggtgatgc ggcaaccgtt attaattacc cgatttcaaa tacctcgtgg
     1861 gcggcacctc agccaacgga tcccgattat aaagtggcat tctccgaaga tgtaaacggt
     1921 aacccgattt acacgtccgg catgactccg gaagagaaat atgaagcggc ccttgaagcg
     1981 gcaaaaggct ttcttaaagc agccggttat acttacgatg aagccaccgg aaaatttacg
     2041 gcggctccgg aaggtgccag cctgacctat gaaattatca ttccggcagg cgggacagga
     2101 gaccacccct cttttgcact tgttacggat gccgccaatg ccttggcaaa aatcgggatc
     2161 acgttgaaga ttaacgatcc cgctgataca aatgttttat gggatacatt ggatgccggt
     2221 aaacaaaact tatggtgcgc cgcttggcag gctacaatcg atcccgatat gtatcaggtt
     2281 tatcacagtt caaatattgt cggtaaaggc ggttcggatt ccaaccacta tcacattgcc
     2341 gatcccgagt tggataaagc aattatagag gctcgccaaa gcgatgacca agcttatcgg
     2401 aaggaaatat ataagacagc cttggatatc atcgttgatt gggcggttga aattccggct
     2461 taccagcgtc aaaattctac aattttcagt accgaacggg ttaagattga cacggtaaca
     2521 ccggacatta ccactttcta tgattggaat gccgaaattc aaacactgga aatggctcaa
     2581 tagaccaaac aaccggatga gatctgcttt aaggcgtttt attctcaaaa acgaatcagg
     2641 gggtggtgcg tcttacacca cccctaattt tcaatgggag aaaaacaaaa attgaaatat
     2701 taatcatttc aaattgaggt tcatcggagc aaaaaccttc atgtttaaat atttcttgcg
     2761 cagattgact tatgcagttt ttattctatt ttttgggtca ttgattgttt ataccgtgat
     2821 ccgctttctc ccgacatcct acgtggaaaa tattgccaga cagcggtcaa atttacccgg
     2881 agcgaaaagt tattcacaga ttctcgcgaa tctaaattct gtatacggct tggacaccga
     2941 tatttttacc ggtttcttaa gatggggaaa aaacgcaatc cacggagact ttggatattc
     3001 ttggcaatat aatattccgg ttgttcaaaa attcaaagaa gtcatttggt actctgtttt
     3061 cttaaatgtg gttacgcttc tcgtggaaac attcatctct attcccctcg ggattttggc
     3121 tgccagaaaa cagtacagta agacagatta tgccgtgacg gttctttcca tgatttgtat
     3181 ttctttaccg acattctttc tggctaccct attaaaatat atattttccg tcaaattagg
     3241 ctggttcgat ttatacggga ttgtcggccg atactacgag caacttgatg catggggaaa
     3301 atttctggat atggcaaaac atcttgtctt gccggtactc accctctcaa tgttgaatat
     3361 cggcggatta atgcggtata cccgtaccaa tatgctggag gtcttgaaca ccgattacat
     3421 tcgtactgct cgcgcaaaag gactttccga agatgtcgtt gtcaaaaaac atgcttttcg
     3481 aaatactttg gtccccctgg tcagttattt ttcatacctt atccccaata tgttttccgg
     3541 ttcgtttatt acggagacat tgtttcaaat ccccggcatc ggatatattt cctattatgc
     3601 ggtgatccat ggcgatattc cgttcgctat gttttattcg gttttaataa tttttctgac
     3661 acaaatcagc ttattgatcg cggattttat gtatgcggtg gtagatcccc gtgttcgaat
     3721 taattaatgg agaccgaaaa tgaaccaaaa tcaaacagga aatagacaaa ccaatggtcc
     3781 gcaaagtgaa gaaattcggt tggatgacga aaagaggatc aaagtactgt caccgacaat
     3841 gttggttttt aaacggttct tacggaataa actcgcaatt gtcggtttgg ttattcttat
     3901 tttaatgttc cttttctctt ttctgggggg tgttctcagt ccctatcaac agcagcaggt
     3961 tttccgtaaa tatgaagtga ttccgaaaga atatgcatct gccacaatca atcaaaatct
     4021 gcggtatatt gttcaagatg gggagatttt ccctaattcc gcaatcgcta aatttgtttt
     4081 ggcgctcaat gaaggacaaa caagattcaa ttcagaggaa acagaatatt cgtataaaaa
     4141 agaaggcgat gatttttatc tgatatcgga aaataagaaa atcgccgatg tcattattct
     4201 tgccggtaaa ggtcgattta cactttcaca aggaattgat tttccggaag agtcacaaaa
     4261 acaagccata gaagcgattc aagccggaaa aaatgaattt gaaagcaatg gaataaaata
     4321 tttcctttcc agaaaaggaa agataacgac catttcaatg ccgaaagaaa ttgcgattgc
     4381 gacgaaatta atttttgatg ctttcgaaaa tgatgcgatt ccttcgttga atcggattga
     4441 attcgtttat caagcacaaa atgccttaat aaataacatc gaacgattca gcgtaaacgg
     4501 agaaaattat cgaatagaaa tttcggaaga ttcatcgatt atctataagg attacacaaa
     4561 cggagaaaga cctttcgctt cgatttcgaa ttttatcgta caaccaaaat cacctgatgt
     4621 tttcttaaca gtcgatttta aaaagaaatt taatgaagca attctttcaa atcaaaattc
     4681 attttccgtt ttatccgatt ccggggagga aattccttat accattaacc gtgtgaatga
     4741 aacttacacc atctatactc agacaaaagc acaattgatt gatatgtaca gccctccatc
     4801 caaagaacat tggcttggaa ccgatgccaa cgggatggat gttctaacgc gattaatgta
     4861 tggaggaaga atatcattaa tggtcggctt tgtcgttgtt ttccttgaat tattcatcgg
     4921 agtaatcgtt ggggggatcg caggctattt tggaaatacc gttgacacat tgctgatgag
     4981 atttgtcgac cttttcaatt cgattccatt ttggccgatg gtaatcatcg ccggatccgt
     5041 gatggatact ttggaagtga agccgactcc gagaattttt gctctgatgg gaattctcgg
     5101 gattttgagt tggaccggca tcgcccgcgt agtcagagga caaattctct cgcttcgtga
     5161 gcaagatttt atggtcgcca cagaagctgc gggcattcgt gtttccaggc gtatttttaa
     5221 acatttggtc cccaatgtaa tgccgttatt aattgttcag gcaactctga gtttgggaag
     5281 cattattata accgaagcga ctttatcttt tttaggactg ggagttaaat accctatcgc
     5341 atcatggggg agcattatta atgccgccac aaatattcac atcatgacaa attactggtt
     5401 tatatggatt ccggccggta ttctgatcgt tctgactgta ttggggttta attttgtggg
     5461 tgacggatta cgtgacgcgt atgatccaaa aatgaaaagg tgacggtaat gaaactattt
     5521 tctgaaaaac ccttgaaaaa agagagcggg aactacattt ccgccagaga atcctacaat
     5581 atctcaaaag aaaacaagcg aattacgaat gcgattgaaa gagagagaaa tcggaaaaat
     5641 gtgccggaat cggaatattt aaccgtaatg cacaatcccg aaaatgtggt ggaatatgac
     5701 aatcttcata cttatttctt cactgatatc ggaacggtaa aagcggttga cggggtttct
     5761 tttgaagttc caaaagggaa aacagtcgga atcgtcggag agtcgggatg cggaaaatca
     5821 atcaccagcc ttt
//
LOCUS       DAOSMM010000086         5608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_14590_length_5608_cov_17.883306, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000086 DAOSMM010000000
VERSION     DAOSMM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14590_length_5608_cov_17.883306"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1613
                     /locus_tag="PK898_09325"
     CDS             <1..>1613
                     /locus_tag="PK898_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20272.1"
                     /translation="EGQQSLIQTRQISSSTVLSAEEGKTIEKVSTARIKGSSDKLTPT
                     PSTSMPSEESEKLTAGTDIAVTGSALIQSTEAVVHNTTSGQLTIADTAIPVVSAPAIR
                     DSQPTENATGVIKEPERSKITAEKTESLKITSTTGVESEKATYNLNFFQKILFFLNPN
                     WVSVPSVTPTVAEPLKITEENPFLNESTSETLVTPEHPEVRGSESPEITEAGNLETGL
                     SGTPIAETVVPKEVNTQEFSQSMTVLPEKERRESVTGTNISVSIENFVEGTPELTRTP
                     APLMPSSISTQPTAKNADTLVPTHVQADSGRTVATAGSGGYLGGTIVVPVISSNDQSA
                     IENPIVSPQAPSSTMVLVEPSSEVVSKSTLTSVSTPSPVPTVEPAQQPNVFQRFFYFI
                     FPGAKNTETVQTPTNLPTVTSTATIGEVQPAQKPNIFQRFITFIIPGRDEPAPTITSV
                     PAVPPTATAPAIEPSSLPAPSLSPTVFIPQRDNSSEETQVPTKQVSSDTEPETSEKIP
                     TTDSSLQHTNDSTNYLNKTENVPSGEPET"
     assembly_gap    1614..1713
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1871..3247)
                     /locus_tag="PK898_09330"
     CDS             complement(1871..3247)
                     /locus_tag="PK898_09330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOU20273.1"
                     /translation="MSITETPSKIEKILQDAAVNRLLPASQICIVRDSEVILSESFGY
                     VPVGSKKIKTQKLTLFDLASVTKLFTATAFMRLVDLNLISLEDAVSKILPEFSGERPI
                     APYEDPLNPGKLISVDHGFTGMVDAGEITFRQLLRHSSGLPAWRPLFTQPTVSAAKQM
                     VFDTFFSYKPDTDVVYSDLGFMLVGWAIEKICGMGLDQAIDHFVLKPLQLTETGFRPL
                     SRDFNTPLPLPAEGIAPTGICAVRHKILVGEVNDENCASLGGISGHAGLFATAEELAA
                     FGQSFLDESSILTSESMNEMKKVQAVSRDGLVRRGIGFQLWSADPNASYAALSPDSFG
                     HTGFTGTCLWVDPQRQMVIAFLTNEVSNGRKNRKFFRCELKFSLKRQQFVRFPPIFPV
                     EKSIEFSNQKSRLQISAFFDLYFYFIECLFLRSLTPRMSAKRTITTKIIIGIFHLSAT
                     PVFGKAVD"
     gene            3808..5595
                     /locus_tag="PK898_09335"
     CDS             3808..5595
                     /locus_tag="PK898_09335"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOU20274.1"
                     /translation="MAKSSNRKILLFLLILFFLLYFPVSTHADEAENCHPDTFFSQSG
                     HCEYEVEHYDIKFSWDDRTNLLNGDVTLKVNALIDAGSIPLDFSNRYLISEVNVDDSP
                     VNYELTEDDLIIEYAITAGSEYKIQISYSGLAEEKQIFRSSSESVTSEIAPFCIVSEP
                     TLAAEWFPCNDKLSDKATFSTSVTVPAKYAVASNGHLSEIRFSDGTVLKPQSPFSYSV
                     TEDTAGTATYFYESEAPMTPYLYTVCIDSFDMDQKEIRPDLTQLDFIQQKLDMKEEFR
                     KWANEIPEMIACFEPIVGKYPFKDAGSIVVNKSFGGALETQTRSVYGNDMSYAGEAGF
                     AHELAHQWIGDLVGIADWSELWIKEGFATYAEALWQKCNGNSEGFEQTLKDDYITMAN
                     LSVQVEDVKQYAKEFTDTYAGRSIELEDPEIVKDAIEAICNLTLDEKAIQTLKGRVSS
                     NPINLQEFWEIVPNYCNMTLVTPLLDQKLLTILGIENQSGTSKKLYGPKSINNTIENM
                     YSLNAYMGGALVYYALFLELGEQKFDKAMQTIVQRYAWDVIDTQEFIAVFSEVAGYDL
                     SGLVNKWLVYDVIPEMPGFPTYEEILKSY"
BASE COUNT         1594 a         1287 c         1162 g         1465 t
ORIGIN      
        1 cagaaggtca acaatccctt attcagactc ggcagataag cagctcaacc gttttatccg
       61 cggaagaagg aaaaacgatc gaaaaggttt ctaccgcccg gattaaggga tcttccgaca
      121 agttgactcc gacaccatct acttcgatgc catctgagga atcggaaaaa ctcacagccg
      181 gtaccgatat cgccgtaacg ggttccgcct tgattcaaag caccgaggcg gttgttcata
      241 atacgacttc gggtcaattg acaattgccg atacggctat ccccgtcgtt tcggctcccg
      301 cgattcgaga ttcacagccg acggaaaatg cgaccggtgt aataaaagaa ccggagcgtt
      361 ctaaaattac cgcagaaaag accgaatctt tgaaaatcac ctcgacaaca ggggtggaat
      421 ctgaaaaagc gacttacaat ttgaattttt tccagaagat tctgtttttc ctgaatccga
      481 actgggtttc tgttccttcg gtaactccca ctgtcgccga accgctgaaa attaccgaag
      541 aaaatccgtt tttgaatgaa tccacatctg aaacgcttgt aactccggaa caccctgaag
      601 tccggggctc ggaaagccct gaaataaccg aagccggcaa tttggaaaca ggtctctctg
      661 gcacaccgat cgctgaaaca gttgtcccga aggaagtgaa tacgcaggaa ttttcacagt
      721 caatgactgt tctgcctgaa aaagagagaa gagaatccgt gacaggcaca aacatttcgg
      781 tttcgatcga gaactttgtt gaagggacac ccgaactgac acggactcct gctccgctga
      841 tgccctcgtc gatttctact cagccgaccg caaaaaacgc ggataccctc gtcccgaccc
      901 atgtacaagc agattccggc cgaactgtcg ccactgccgg ttccggcggt tatttgggcg
      961 gaacaatcgt tgtgccggtc atttcgtcga atgatcaatc cgccattgaa aacccgattg
     1021 tctcgccgca ggcaccttcc tcaacgatgg tccttgtaga gccttcttcc gaggttgtat
     1081 cgaaatcaac tctaacctct gtttcgacac cttcccctgt tccgactgtt gaacccgcgc
     1141 aacaaccgaa tgttttccaa cgctttttct atttcatttt cccgggcgcg aagaatacgg
     1201 agacggttca gacaccgaca aatttgccta cggtaacttc aaccgcaacg atcggcgaag
     1261 tgcagccggc tcagaaaccg aatatttttc aaagattcat aacttttatc attcccggca
     1321 gagatgaacc ggcaccgacg atcacttccg tgccggcggt accgcctaca gctactgcac
     1381 cggcgattga gccaagttct ttacccgctc cttctctttc tccgactgtc ttcattccgc
     1441 aacgggataa ttcatcggaa gaaactcaag ttccgacaaa acaggtatcc tccgatacgg
     1501 agccggaaac atccgaaaaa attccgacaa cggactcttc gttgcaacat acaaatgatt
     1561 cgacaaatta tttgaataaa actgagaacg taccttccgg agaaccggaa acannnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaccgga aacaggcacc gtcattccgg
     1741 ttcaaaatga ttctgaaaat cgaatcgatt tgaatccgcg gttgatcatg tttgggagta
     1801 ctcctaccgc cgaagctacc gaaatacttg tcgttatcgg aaaaaatgtc gggacggaag
     1861 ttgcggtaac tcaatccacc gccttgccaa aaactggtgt cgccgacaaa tggaatattc
     1921 cgatgatgat tttagtggtg atggttcttt tggcgctcat tctcggcgtc agactcctta
     1981 ggaataagca ttcgataaaa tagaaataca aatcaaaaaa agccgatatt tgtaatcggc
     2041 ttttttgatt cgaaaattcg atcgattttt caaccgggaa tatcggcgga aaccggacaa
     2101 attgctgccg cttcagtgag aatttgagct cgcaacggaa gaattttcga ttctttcgtc
     2161 cgttggaaac ttcgttcgtc aagaatgcaa taaccatttg acgctgggga tcgacccata
     2221 agcatgttcc ggtaaaacct gtatgcccaa aagaatccgg acttaaagcc gcataactgg
     2281 cattcggatc agccgaccat aattgaaagc cgattcctcg tcggaccaag ccatcccggc
     2341 tgacggcttg aacttttttc atttcattca tgctttccga tgtcaggata gaagattcat
     2401 ccaagaagga ttgtccgaaa gcagccagct cttctgcggt agcgaacaaa ccggcatgcc
     2461 cggatattcc tcccaaactg gcacaattct catcattcac ttcgccgacc aagatcttat
     2521 gccgaaccgc acaaataccg gtcggagcaa ttccttccgc cggaagcggt aagggtgtat
     2581 tgaaatcacg tgataagggg cgaaaacccg tttcagtcaa ctgaagcggt ttcaaaacaa
     2641 aatgatcaat cgcctgatcc agacccatac cgcaaatttt ttcgattgcc cacccaacta
     2701 acataaaacc gagatcggaa tatactacat cggtatccgg tttataactg aaaaaagtat
     2761 caaaaaccat ttgttttgcg gcggaaacag tgggctgggt aaacaacgga cgccaggcgg
     2821 gtaacccgga tgaatggcgg aggagctgac gaaacgtaat ttctccggca tcgaccattc
     2881 ccgtaaaacc atgatcgacc gatattaact ttcccggatt gagcggatct tcatatggag
     2941 cgatcggccg ttccccgctg aattccggaa ggatttttga aaccgcatct tccaaagaaa
     3001 tgaggttcaa atctaccaat cgcataaaag ccgtggcagt aaagagtttt gtcaccgaag
     3061 ccaaatcgaa taaagtcagc ttctgagtct ttatcttttt ggaaccgacc ggcacatacc
     3121 cgaaagattc ggaaaggatt acttcggagt cgcgaacaat acaaatttgc gaagcgggta
     3181 acaatctgtt tacggcggcg tcttgcagga tcttctcaat tttcgaagga gtttcagtga
     3241 tactcaatta tcttatcgct ttcttaatcg ccatacttca gtccgaaaaa tatcaacata
     3301 aaagattata tttactaaac aaaagtagtt taattaaaga ctgtttttta gcgtaataga
     3361 aaattttatt tctgtcaaat aaaaaccttc ccctttataa cgtgacattt attctgatat
     3421 ttaaaaactt attcgaaatt gcagtttccg attcactttt gggacggttt tgctcccgca
     3481 gatctcctcc taaggaaatc aaaaacaaat tcatatttgc gaccggtctc ttttttaaag
     3541 aaattcagag aaaagtcctc tcactcctct ctccttccgt tatcttatat tgtttcattt
     3601 cgccgctcga aaacacttcc ggaattattt cccgaagaca ttattccttc cttacctctc
     3661 ggtatccttt tttattgaaa gaagctgaga gggcttcttt ctcgcatccc cctctctccg
     3721 ttctcggtat cttttatcgt ctcattttaa ataaccggaa taaaagtaaa attaacttat
     3781 cttcaatctt gaagggaaag tgctaaaatg gcgaaatctt cgaatcgaaa aattcttctc
     3841 ttcctactca tcctcttttt cttgttatat ttccctgttt ccacccacgc tgatgaagcc
     3901 gaaaactgcc atccagacac ttttttctca caatccggcc attgcgaata tgaagttgag
     3961 cattacgata ttaagttctc ctgggatgat cgaacaaact tattgaatgg agatgtaact
     4021 ttaaaagtaa atgcgctcat cgatgccggt tcaattcctc tcgatttcag taatcgttat
     4081 ttgatcagtg aagtgaacgt ggatgacagc ccggtcaatt atgaattgac agaagatgat
     4141 ctgatcatcg aatatgctat aacagccggt agcgaataca aaattcaaat cagttattcg
     4201 ggattagccg aagaaaaaca aatttttcga tcatcctccg aatcggtcac ctcagagatt
     4261 gctccgtttt gtatcgtttc cgaaccgact ctggctgccg aatggttccc ctgcaatgat
     4321 aagctgagtg acaaagccac tttttcaact tccgttaccg ttcccgcaaa atatgccgtc
     4381 gcaagcaacg gtcatttatc cgagattcgc ttttccgacg gaacggtatt aaaaccgcaa
     4441 agtccctttt cctattccgt aaccgaagat acggcgggga ctgctactta cttctatgaa
     4501 tcggaagcgc ccatgacacc ttatctgtat acggtctgta tcgactcctt tgatatggat
     4561 cagaaagaga ttcgaccgga tttaactcaa ctcgatttta tccaacaaaa attggatatg
     4621 aaagaagaat tccgcaaatg ggcgaatgag atacctgaaa tgattgcctg ttttgaaccg
     4681 attgtgggga aatatccgtt caaagatgcg gggagtattg tggttaacaa atccttcggc
     4741 ggcgctctcg aaactcaaac ccgttccgtc tacggaaatg atatgagtta tgccggagaa
     4801 gctggattcg cccatgaatt ggcacatcaa tggatcggtg atttggttgg aattgcggat
     4861 tggagtgaac tttggatcaa agaagggttt gcaacctatg cggaagcgtt atggcaaaaa
     4921 tgtaacggaa attctgaagg gtttgaacaa acgctgaagg atgattatat aaccatggct
     4981 aatttatcgg ttcaggttga agatgtaaag caatatgcaa aggaatttac cgatacttat
     5041 gccggtcgat cgatcgaatt ggaagatccg gaaatcgtga aagatgctat cgaagcgatt
     5101 tgtaatctca ctttagatga aaaagccatc caaacattga aaggaagagt ttcttcaaac
     5161 ccgataaacc ttcaggaatt ttgggaaatt gtcccgaatt attgcaatat gactctggtc
     5221 actccccttc tggatcaaaa actcctgacg attttgggta ttgaaaacca atccggcaca
     5281 tcaaaaaagc tatatgggcc gaaatcgatt aataatacga tagagaatat gtatagttta
     5341 aatgcctata tgggtggagc gctggtttat tatgcactct ttttggaact gggtgagcag
     5401 aaattcgaca aagcgatgca gacgattgtc caacgctatg catgggatgt aatcgatacc
     5461 caagagttta tcgcggtatt cagcgaagtc gcgggttatg atttatccgg cctggtgaat
     5521 aaatggctgg tttatgatgt gattccggaa atgccggggt tcccgaccta tgaagaaatt
     5581 ctaaaatcat attaatgttt ggaattcc
//
LOCUS       DAOSMM010000087         5538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_14812_length_5538_cov_15.946744, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000087 DAOSMM010000000
VERSION     DAOSMM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14812_length_5538_cov_15.946744"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1978)
                     /locus_tag="PK898_09340"
     CDS             complement(167..1978)
                     /locus_tag="PK898_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU20275.1"
                     /translation="MTEKSKDVYLGMGAIVHEGGVAFRVWAPHADAVFVKGTFNNWSE
                     SADPMKAEEHGTWYTDIPHAKVGDEYKFVLISGNQKLERMDPYARQVTNSAGNCVIYD
                     PKAFDWEGDRFELPPFNELVIYEMHIGSFFADSDKKPGDFDSAAEKMDHLKRLGVNAI
                     QVMPVAEFAGDYSWGYNPANIFAVESAYGGPDGFKKFIREAHQRGFAVIQDVVYNHFG
                     PGDLSLWQFDGWQENGKGGIYFYNDDRSETPWGSTRPDYGRGEVRSYILDNVQMWLEE
                     YHVDGLRFDSTLYIRTIDGSEGNDLPDGWSLLQAINEKIRAYSTHVISIAEDLRSNEW
                     ITKEISENGAGFHAQWDANFVHPVRDAVTVADDSARSMDVIRNAVQFKYENDAFRRVI
                     YSESHDEVANGKARVPQEINPDDPTGVFAQKRSTLAAGLVFTAPGIPMLFQGQEFLEG
                     DYFRDDVPLDWDLNTEFSGIVRLYRDLIRLRSNQKNQTRGLCGQFLNVFHVNEEEKVL
                     AFQRWDQHGTGDDVIAVFNFGNAGKTDYVIGLPTKGIWKLRLNSDAKIYSKDFEGFFS
                     NDTEGFEGGKDGFDFHGSVNIGPYSLLIFSQERNGNH"
     gene            complement(2256..3221)
                     /locus_tag="PK898_09345"
     CDS             complement(2256..3221)
                     /locus_tag="PK898_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOU20276.1"
                     /translation="MTRVMFNRMVTDEQKSRLTKDFPEVDFVFTDSVADPKLLASEGA
                     KSDVLVGVESPDLLNAILSVPNQVRWLHSWFAGVNHLPLDLLEEHHVWLTRAGGVAVN
                     PIVETTICLMIMLTRNFRKYGKQQDAHQWIQLEWPDEVHGKTIGILGMGIIGKGIAKM
                     AKAFDMEVWGLQQFPQPNEYTDHIVGNDELDKLLNAADYVVNILPLTAATKYLMNEER
                     FNQMKKGAYYLNVGRGATTDTQALIEALQSGKIKGAGLDVIDVEPLPPDSPLWDMDQV
                     IILAHQAGFTTQYDERTLQLFERNLRNYLEKGKPNENLVNLEAGY"
     gene            complement(3319..4113)
                     /locus_tag="PK898_09350"
     CDS             complement(3319..4113)
                     /locus_tag="PK898_09350"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOU20277.1"
                     /translation="MENKAQIVYFSHGGGPLPILGEPNHRAMIDFMKNLPSRLNRPEA
                     ILVISAHWEEQHPTVLGASHPSMFYDYYGFPEKAYQIQYPAPGDPELAKRIVGMLEKN
                     HIQARIDLNRGFDHGLFIPLELMYPEANIPSIQLSLIRGLDSAAHVSLGKALRDLVSE
                     NILILGSGFSFHNQLAFFDSQSEYADPENDAFQNWLIETCTGNFSQEEREQRLIEWEE
                     APYARYCHPREEHLLPLMVCQGLANKPAKLIFDDAILRKRCIAFEW"
     gene            complement(4146..4769)
                     /locus_tag="PK898_09355"
     CDS             complement(4146..4769)
                     /locus_tag="PK898_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOU20278.1"
                     /translation="MDLIEMVIYSDYICPWCYVGQGVVEKLRKEYPMAITWRPYFLRP
                     DIPPEGMDIPEELNDHMAEIGERLKQMADAAGLEIVFPKHLPNTRFAHEATEYADQKG
                     KGFEFHREVFARFYGKGEDIGQWQVLQSAAKAVGLDADEMKRQVEEGKYTQAVQDHIA
                     EAVKWGIESVPTYIINNRYAIVGAQPYDRFLEVIQKIDLPHNQESIQ"
     gene            5240..5401
                     /locus_tag="PK898_09360"
     CDS             5240..5401
                     /locus_tag="PK898_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOU20279.1"
                     /translation="MKKYKCSVCGYIYDPAKGDPDSGIKPGTAFEDLPDDWACPVCGS
                     SKDKFRPMD"
BASE COUNT         1555 a         1345 c         1001 g         1637 t
ORIGIN      
        1 aaaatactac cgttcctatc ggcgatatca aaaaatagac aagatcgact gtttctgcga
       61 atcaattcac aggggcaata cagtcttgtg gaaaaccgcg tcatatatgt gatattccca
      121 tcatagcaaa ttcaaaattc gattcatccg ggttcccgat aatgattcaa tgattcccat
      181 tccgctcttg actgaaaatc aacaaactgt aaggtccgat attgacagat ccatgaaagt
      241 caaatccgtc ctttccgcct tcgaaacctt ccgtgtcgtt gctgaaaaat ccctcaaaat
      301 ctttactgta tatttttgcg tcactgttta aacgcaactt ccaaatccct tttgtcggca
      361 gtccgatcac ataatcggtt ttgcccgcat ttccgaaatt gaagaccgct attacatcat
      421 cacccgtacc gtgttgatcc caacgctgaa atgccagtac cttttcctcc tcgtttacat
      481 ggaagacatt taaaaattgt ccgcacaaac cccgagtttg atttttttga ttcgatcgta
      541 accgtattaa atcgcgataa aggcgaacga tcccgctgaa ttctgtgttt aaatcccaat
      601 ccaatggcac atcatcccga aaataatccc cttcaagaaa ttcctgtcct tgaaacaaca
      661 taggaatacc cggagcggta aaaaccaaac cggcagcaag cgtggaacgt ttttgtgcaa
      721 acacaccggt cggatcgtcc ggattaattt cctgcggaac ccgtgctttt ccgttcgcta
      781 cttcatcatg ggattcactg taaatcaccc gccgaaacgc atcgttttca tatttgaact
      841 gaaccgcatt tcggatcaca tccattgaac gggcggaatc atccgccacc gtcaccgcgt
      901 cgcgaaccgg atgaacaaaa ttagcgtccc attgggcatg aaaaccggct ccgttttcac
      961 tgatttcttt ggtgatccat tcgttgcttc gtaaatcctc ggcgatggaa ataacatggg
     1021 tggaatatgc ccggattttt tcgttaatgg cttgcagtaa agaccaaccg tccggcaaat
     1081 cgtttccttc tgaaccgtca atcgtgcgaa tataaagtgt actgtcaaag cgtaaaccgt
     1141 cgacgtgata ctcttccaac cacatttgaa cattatccaa gatatagctg cgaacttctc
     1201 ctctgccata atccggccgt gtacttcccc agggcgtttc ggaacggtca tcgttgtaaa
     1261 aatagattcc gcctttgccg ttttcctgcc acccgtcaaa ttgccaaaga gataaatctc
     1321 cgggtccgaa atggttataa accacatcct gaatcactgc gaatccgcgt tgatgcgctt
     1381 cacgaataaa ctttttaaac ccgtccggac ctccataagc actttctacg gcgaaaatat
     1441 tcgcaggatt gtatccccaa gagtaatctc cggcaaattc ggcaaccggc atgacttgaa
     1501 tggcattcac ccctaaccgc tttaaatgat ccatcttttc cgctgccgaa tcaaaatcac
     1561 cgggcttttt atcggaatcg gcgaaaaagg atccgatatg catttcataa atgaccagtt
     1621 cattaaaagg aggaagttca aaacgatccc cttcccaatc aaaagccttg ggatcataaa
     1681 tgacacaatt tccggcggaa tttgtcacct gacgcgcata gggatccatt cgttccaatt
     1741 tttgatttcc gctgattagg acgaatttat attcatcccc cactttggca tgtgggatat
     1801 ccgtatacca agtcccatgc tcttccgcct tcatcggatc agcggattcg gaccaattat
     1861 taaaagttcc ttttacaaaa acagcatcgg catggggagc ccagacccga aaagccacgc
     1921 ctccttcatg aacaatcgca cccattccaa gataaacatc ttttgatttc tcagtcactt
     1981 aatcatcctt cattcgcaat ttttttgccg gttaattttt tgaatccgta aaaaagatcc
     2041 cgaaagataa tcgatatatc ttttaccggg atctttcgtt ttttttcgcc aagttcggat
     2101 caatccgatc agctaaaaac attgaaacga tttgactctt tcatttttct cgtttaagca
     2161 cacctcagtc gcatgaaaaa gtctttgatc atttcatccg ttcgtcgatc aggttgaacg
     2221 gatgaaatgg tttttgttat gaaattgctc gccgtttaat atcccgcttc cagattgacc
     2281 agattttcat tcggctttcc tttttcgaga taatttctca ggtttctttc aaaaagttgc
     2341 agcgttcgtt catcgtattg cgtcgtaaaa cctgcttgat gagccaaaat aattacctga
     2401 tccatatccc aaagcggact atcggggggt agcggctcca catcgattac atccagtccg
     2461 gctcctttga tttttccgct ttgcagtgct tcgatcaaag cttgggtatc cgtcgtagct
     2521 cccctcccga cgttcaaata ataggcacct ttcttcattt gattaaagcg ttcttcattc
     2581 atcagatatt tcgtcgctgc cgtgaggggc aaaatattaa caacataatc cgccgcattc
     2641 agcagcttgt ccaattcgtc attcccgaca atatgatcgg tatattcatt cggctgcgga
     2701 aactgttgta aaccccaaac ttccatatcg aaagcctttg ccattttcgc gataccttta
     2761 ccgatgattc ccatgcccag aattccgatt gtctttccgt gaacctcatc cggccattcc
     2821 aattgaatcc attggtgagc atcctgttgc ttcccatact tacgaaaatt tcgagtcagc
     2881 ataatcatca ggcaaatggt tgtttccaca atcggattca ccgccacacc acccgctcgg
     2941 gttaaccaaa catgatgctc ttccaaaaga tccagcggca aatgattgac tcccgcaaac
     3001 caagaatgca gccaccggac ttgattggga accgataaaa tagcattcaa taaatcgggg
     3061 gactccactc cgaccaaaac atcacttttg gcaccttccg aagccaataa cttcggatcg
     3121 gcgacagaat cggtaaagac aaaatcaact tccggaaaat cttttgttaa acgacttttc
     3181 tgttcatcgg taaccatcct attaaacatg acacgtgtca tttttccctc ctctcttcct
     3241 tcataagacc ttattatttc gaatacttta cgattcggtt ccgctattct ttaattttat
     3301 tatatatgat ttgtcaaatt accactcaaa ggcaatgcat cgttttctca gaatcgcatc
     3361 atcgaagatt aattttgcgg gtttgtttgc cagcccctga caaaccatca aaggaagtaa
     3421 atgttcttcg cgcggatgac aatacctggc ataaggcgct tcttcccatt cgattaatct
     3481 ttgttcgcgt tcttcctgag agaagttacc ggtgcaggtt tcgatcagcc aattttgaaa
     3541 agcatcattt tccggatccg catattccga ttgagaatca aaaaaagcaa gttgattgtg
     3601 aaaagaaaat ccggaaccga ggatcaagat attctcggaa accaaatcac ggagcgcttt
     3661 tcccaaagaa acatgagcag ccgaatccaa tccacgaatc aaagaaagtt gtatcgaggg
     3721 aatatttgcc tccggataca ttaattccaa cgggataaac aatccgtgat caaatccgcg
     3781 attcaaatca attcttgctt gaatatgatt tttttccagc attccgacaa tccgttttgc
     3841 gagttccgga tcccccggcg caggatattg gatctgatat gctttttccg gaaaaccgta
     3901 ataatcatag aacatggacg gatgcgatgc tcctaaaaca gtggggtgct gctcttccca
     3961 atgagcgctg atcaccaata tcgcctccgg cctattcaac cgggacggta agtttttcat
     4021 aaaatcgatc atcgcccgat gattcggttc tcctaaaatg ggcagagggc ctccgccatg
     4081 ggaaaagtag acgatttgag ctttattttc catttatttt cgttcctttt caaaagtcct
     4141 atatttcatt gaattgactc ttgattatga ggcaaatcga ttttttgaat aacctcgaga
     4201 aaacgatcat agggttgagc tccgacgatc gcatagcggt tatttatgat atacgtggga
     4261 acactttcga taccccattt tacggcttcc gcaatatgat cttgaaccgc ttgagtatat
     4321 tttccttctt caacctgtcg cttcatctca tccgcatcca acccgacagc tttcgcagcg
     4381 gattgaagta cttgccattg tccgatatcc tctcctttac cgtaaaaacg agcaaaaact
     4441 tcgcgatgaa attcaaaccc ttttcccttt tgatcggcat attctgtggc ttcatgggcg
     4501 aaccgggtat tgggcagatg tttcggaaaa actatctcca aaccggctgc gtcagccatc
     4561 tgctttaatc tttcaccgat ttctgccatg tgatcattca attcttccgg aatatccatc
     4621 ccttccggcg gaatatcggg tcgaaggaaa taaggtcgcc aagtgatcgc catcggatat
     4681 tcttttctca gtttttcgac aacaccctgc ccgacatagc accaggggca gatataatcg
     4741 gaataaatta ccatttctat caagtccatc ccataatctc ccattgaaaa cataccataa
     4801 tcagcactcg ttaattattt accgaggtta aaccttactt attatcatgc cacaattttc
     4861 cgagatgaac aataatccgt aattcgatca tacggataaa tttgcataac ttattcttct
     4921 cctcattcaa gtgacggcat tctcccgaaa atccatagct tttttagatt ttcagagaaa
     4981 ttcttaaagc cggttgtttt tttaccgttt tcaaatttaa ggaatcaata agccttccga
     5041 aaataacggc tgcggaatcg tatttataaa gattttttca ataattgaat attccaaaac
     5101 ttcatttggg cttcgatccg catcggcaat gataagaaga atcttttctt gtttgatcga
     5161 atttcagctg tcatttttta atcaagctta taatcgaaat tagaatcatt acaactttta
     5221 aaaaaggagt ttaaaaaaaa tgaaaaaata taaatgcagc gtatgcggtt atatttatga
     5281 ccctgcaaaa ggagatcccg attcgggaat taaaccgggg acagctttcg aagaccttcc
     5341 ggatgactgg gcttgcccgg tttgcggatc ttccaaagat aagttcagac cgatggatta
     5401 aactataaac tttaaccatc aaaaaatgac aaatctaatg gtttagtgat aaactctttt
     5461 atgaattgac gataatttgt tgatttttta tcggatctct gtatcttatt acatcagagt
     5521 caaatttcat gaaatttt
//
LOCUS       DAOSMM010000088         5467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_15040_length_5467_cov_15.896526, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000088 DAOSMM010000000
VERSION     DAOSMM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15040_length_5467_cov_15.896526"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..2399)
                     /locus_tag="PK898_09365"
     CDS             complement(228..2399)
                     /locus_tag="PK898_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU20280.1"
                     /translation="MIEKLLSNSSYSFNIYALPMFLTATSIILLGVYVLLQEKSVVTK
                     AFFFSTLSAGIWQLGTGLIYLMKDHALVVWFYKVFVFLGIVAIAPSIYMLTVSVLGLF
                     ESKKKYIALSYVIALFFYLSDLLTDSLTTGVKERFWGPYITYGILSAPFLIFFSFLMG
                     RSIYLSSKIYRGMKSGISRDQIKLHLISLIIALSAAVDFFSCYLPMDVYPFGYLAILS
                     VVFLQAYAIITYQKASLSEIFNSIEDGIVVTDNEGRITNINTSVERFTGIGAQTLSER
                     RLKDLINTYRNKFEDPKQVEEFLQKIRENPNRIVEGDIGLRNPAIQLDIKYSRISDRF
                     GIKTGSVIVLRDITQRKKVEQELREYKEELETRVEQRTQELKQSEEKYRALVSHAQVG
                     IGIHKKGRMIFTNAQFRTMLGYTEEEMTNMEIAQLIHPDEVKLVLKRAWDRYNGKDVI
                     DTYDIQFLKKDGSVLPAIISNVVIDYQGERATLLTVVDTTETKMRRELEIANQELEMF
                     AYSVSHDLRAPLRTIDGFSQALLEDYQDKLDEEGKDYLMRIRAASQRMGLLINSILQL
                     SRLGRSEMRLEEVDLSGLAQNIAAELETNDPDRKVDFVIEKDVKGIGDKTLLRAVMEN
                     LLGNAWKFTQKQAEAKIEFGKTEKEGKTIYFIRDNGVGFDMKYANKLFIPFQRLHSDS
                     EFAGTGVGLTLVQRIVRRHGGDIWVESAVEKGATFYFTLER"
     gene            3013..4242
                     /locus_tag="PK898_09370"
     CDS             3013..4242
                     /locus_tag="PK898_09370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU20281.1"
                     /translation="MKIALFHYSAPPIIGGVESVIAHHARLMTANGHRVTIFAGRGET
                     FDPAIPVKVFPVLDSKNPEALTVKSELDKGLLSERFYSLCDQIQEMLNNELPDFDVLI
                     AHNVTSLNKNLPLTAALFQNYRQPGFPRLIRWHHDLAWSSPRYRNELFDQYPWDLLRK
                     KWADTTDVVVSQARRQELAQLMGLSAENISVIPNGVDLFSFFKFNEQTIRLIRTLNLS
                     EMDPLFLLPARLIPRKNIEFALQIMKAFIVNNPNAGLLITGPEDPHDPENALYRQNLM
                     RMRNEMGLQNTVHFLADVTDDFLPDEIISDFYRLADALLFPSFEEGFGIPLIEAGFEN
                     IPVFCSDIPVFHEIGGENLSYFDPNGDPSAVAKMIDDRLDSEMTFRWSRNAKNNYRWE
                     SIYRSLIEPLITEERKK"
     gene            4239..>5467
                     /locus_tag="PK898_09375"
     CDS             4239..>5467
                     /locus_tag="PK898_09375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU20282.1"
                     /translation="MKTPFRKLKTVIVPIGRDGSGEAALSLAKAIANEVILVGVVSVH
                     NGESISAGTGAARSLRKRLLELSDSPKIRFKSTIIVSDSPWKDLMNVISDEKPQSVLI
                     EWKDDKLTEGLPVNEVLSNSLVNVLIFRSDLPIHYERTMVAVRGGPHAELALKVAMTL
                     KPNHLDVLHISRSGASDDAPYKGYLQILNQLPEINQRFIVTDDVSKTLFQEADRYDLV
                     ILGTTATNALNGPIIGTVAEQMLRNSKSAVLITKIRQEKNPPIFDESAGYRAISVLVD
                     KWFAENTFHSDEFADLDRLMQLKESSNLTVSLAMPALNEEKTVGNVISTIKTALMDNV
                     PLIDEMILVDSNSTDHTREIAEDLGIKTYIHQQLLPESGANEGKGEALWKSLLVTSGD
                     IIAWIDTDIVNIRPHFV"
BASE COUNT         1569 a         1226 c         1068 g         1604 t
ORIGIN      
        1 acgacaacat tatccgtcaa attgcacttt cgaaagaccc gttttgccaa cgttatatcg
       61 tcaggattat cttcaaccaa cataattatt cgtttcatat attgatcggg ccctttctgc
      121 attatagcag cgtataccga ttgaccaacg tttctttctg tattctcgtt tcaatacagg
      181 gtttcgaaaa taatttttcc aaatattaat tttactagca ttttctttca tctttcaaga
      241 gtaaaataaa aagtcgcgcc tttctcaaca gcgctttcta cccaaatatc tcctccatgg
      301 cggcggacga tccgctgaac caaagtcagc ccgacacccg tgccggcaaa ttccgagtca
      361 ctgtgaagac gctgaaacgg aataaaaagt ttattcgcat atttcatatc gaagcccaca
      421 ccgttatcac gtataaaata aatcgtcttg ccttctttct ccgtcttacc gaactctatt
      481 ttcgcttcgg cctgtttttg agtaaacttc catgcgtttc cgagcagatt ctccatcaca
      541 gcccgcaaaa gtgtcttatc gccaattcct tttacgtctt tttcaattac aaagtctacc
      601 tttcggtccg ggtcatttgt ctccaattct gcggcgatat tctgcgctaa cccgctcaaa
      661 tccacttctt caagtctcat ttcacttctg cccaatcgag acagctgaag aatcgaattg
      721 atgagcaacc ccattcgttg gctggcagcc cgaatccgca ttaaataatc ttttccttcc
      781 tcatccagct tatcttgata atcttccaac aatgcctgac tgaacccatc gatcgttctg
      841 agaggagccc tgagatcatg tgaaacggaa taagcgaaca tttccaattc ctgattggca
      901 atttcgagct ctctacgcat ttttgtttcc gtagtgtcca caacggttaa aagcgtcgcc
      961 ctctctccct gataatcgat tactacgttg ctgataatag cgggcaaaac actgccgtct
     1021 tttttgagaa actgaatatc atatgtgtct ataacatctt taccgttata acgatcccaa
     1081 gcccttttta ataccaactt cacttcatcc ggatgaatta attgagcgat ttccatattc
     1141 gtcatctctt cttccgtata tcccagcatt gttctgaatt gtgcgttggt aaaaatcatc
     1201 cggccttttt tatgaatgcc gatacctacc tgagcgtggc tgacgagtgc tctgtatttt
     1261 tcctccgatt gtttcagttc ctgcgtccgt tgttcaacgc gcgtttcaag ttcctcttta
     1321 tactctctta attcctgctc gaccttcttt cgttgggtga tatctctcag tacgatcacg
     1381 gatcccgttt taattccgaa ccgatcactg atacgagaat atttgatgtc taactgtatg
     1441 gcgggatttc gtaagccgat atcaccttcc acaatccgat tgggattttc tcgaatcttt
     1501 tgtaaaaatt cttcaacctg cttgggatct tcgaatttgt tccgataagt attgatcaaa
     1561 tctttcagcc ttctttcgga gagcgtctgg gctccgatgc ctgtaaatct ttccaccgat
     1621 gtatttatat ttgttattct tccttcattg tccgtaacta caattccgtc ttctatcgaa
     1681 ttaaatattt cactcagaga agctttttga taggtgataa tcgcataagc ctgaagaaat
     1741 accaccgaaa ggattgccag atatccaaac ggatatacat ccatcggtaa atagcaagag
     1801 aaaaaatcca ccgctgccga taaagcaatt atgagagaaa tgagatgcaa cttgatttga
     1861 tcgcggctta tcccggattt cattcctcga tagatcttag agctcagata gatggaacga
     1921 cccatcaaaa aagaaaagaa tatcaaaaag ggtgccgata aaattccgta tgtgatataa
     1981 ggaccccaga atcgttcttt gacaccggtt gtcaggctgt cggttaataa gtctgataag
     2041 tagaagaaca aagcgatcac ataacttaac gcaatatatt ttttcttgct ttcaaacaat
     2101 cccaaaaccg atacggtaag catatagatg gaaggcgcga tcgcaacgat tcccagaaaa
     2161 acaaaaactt tataaaacca aacaaccaga gcgtgatctt tcatcagata gatgagaccg
     2221 gtaccgagtt gccagattcc cgcgcttaat gtgctgaaaa aaaatgcctt tgttacgaca
     2281 cttttttcct gtaacagcac ataaactcct aacaggatga tcgacgtggc agtcaggaac
     2341 atcggtagtg cataaatatt aaaagaatag ctggaattgc tcagaagttt ctcaatcata
     2401 cgaatacgaa tcaatccttt tttttaggag tatttgtctg ttacaaaaaa tcgtgaaaat
     2461 ttttacatcc ggtaattctc ttgatcattt ttatagccat accttgtcga agcagaaagt
     2521 tcttctttgt gtatttcaat cgataaaaaa atattgacga attaccggat gtaattatac
     2581 atttatctcc gaaagaaaac agtgtgacaa ccgataatcc tacatagtct ttcagttatc
     2641 gaattgtttc gaaaattgat tgactgcttg taaaccggat gacaaaatat atttttctgc
     2701 gaaagccgtt gaaatcaaaa cgattcgaaa actgtggtat aaaaaacaac agggatttcc
     2761 gtcgattaag aaatccaaaa atttgaggag aatagtaaag ggcaatattt catcaagaat
     2821 tttttattat ccggatccga accactcgat atgaaaatag gaattttttt cagacagacc
     2881 cgatccgggt gattcaaata cagaacaaac aaatcattag aaaatttaaa aatccttttc
     2941 ccgcccttat actattcatt ctgtttttcg ataaaaagac aggtttgagt aatattttgg
     3001 cggtaaatta atttgaaaat tgcattattc cattattccg ctcctccgat cataggcggt
     3061 gtcgaaagcg tcattgctca ccatgctcgg ttgatgacgg cgaacggtca ccgggtgacg
     3121 atattcgccg gacgaggtga gacttttgac cccgctattc cggtaaaagt ttttcccgtg
     3181 ctggactcta agaatcctga ggcattgacc gtaaaatccg aattggacaa aggtttgctt
     3241 tccgagcggt tctattcctt atgcgatcaa attcaagaga tgttaaacaa cgaacttccg
     3301 gactttgatg tcctgatagc tcataatgtt acatcgctga ataaaaacct tccgttaacg
     3361 gctgcattgt ttcaaaacta tcgtcaaccg ggatttcccc gtctcattcg ctggcaccac
     3421 gatctggcat ggtcctcacc ccgttaccgg aatgaattat ttgatcaata tccctgggac
     3481 ctgctgcgga aaaaatgggc ggataccacc gatgtcgtgg tttcacaagc gcgcagacaa
     3541 gaattagcgc aattgatggg attatcagcg gaaaatattt cggtaattcc caatggagtg
     3601 gatctgtttt ccttctttaa attcaacgag caaacaattc gtttgatccg gactctgaat
     3661 ctctcggaaa tggatcctct tttcctattg ccggcacgct taatccctcg aaaaaatatc
     3721 gaattcgctt tgcaaatcat gaaggcattt atcgtaaaca atcccaatgc cgggctattg
     3781 attaccggtc ccgaagatcc ccacgatccc gaaaatgcgc tttaccggca aaatttgatg
     3841 cggatgcgaa atgaaatggg gcttcaaaat accgttcatt ttcttgccga tgttaccgat
     3901 gattttttgc cggatgaaat catttccgat ttctatcggt tggctgatgc gttacttttc
     3961 ccgagtttcg aagaaggttt cggcatcccg ttgattgaag ccggcttcga gaacatccct
     4021 gttttttgtt ccgacattcc cgtctttcat gagatcggcg gagaaaattt gagctatttc
     4081 gatccgaacg gtgatccgtc cgctgtcgca aagatgatcg atgatcgttt ggattcggaa
     4141 atgacttttc gctggtcaag gaacgccaaa aataattaca gatgggaatc aatttatcgt
     4201 tccttgatcg aaccattaat aaccgaggag agaaaaaaat gaaaactcct tttcgcaaat
     4261 taaaaaccgt aattgtcccg atcggaagag acgggagcgg agaagctgct ctgtcgctgg
     4321 caaaagcaat cgccaacgaa gtcattctgg tgggagtagt ctccgtccat aacggagaat
     4381 ccatcagcgc cggaacggga gcggcccgat ccttacgaaa aagattgctt gagctcagtg
     4441 acagtcctaa aatacgtttt aaatcaacga taatcgtctc tgattcacct tggaaagatt
     4501 taatgaatgt tatctcggat gaaaaaccgc aatcggtttt gatcgaatgg aaggatgaca
     4561 aactcaccga agggctgccg gttaatgaag ttctttccaa ttcattagtg aatgtcctga
     4621 tatttcgcag tgacttgccc attcattacg aacggacgat ggtggctgtc cgcggcggcc
     4681 cgcacgccga attggctttg aaagttgcga tgaccttaaa gccgaatcac ttggatgtcc
     4741 ttcatatttc tcgttccgga gcatccgatg atgcccccta taaaggatac cttcaaatct
     4801 tgaaccagct ccccgaaata aaccagcgat tcattgtcac cgatgatgtt tccaaaacgt
     4861 tatttcaaga ggctgaccgg tatgatctcg taattttggg tactaccgca acgaatgctt
     4921 tgaacggacc aataatcggt acggtagccg aacagatgtt acggaattca aaatctgcgg
     4981 tattaatcac aaaaatacga caggagaaaa acccgccgat ttttgatgaa agcgccggat
     5041 atcgggcaat ttcggttctg gtcgataaat ggttcgccga gaacactttt cacagcgatg
     5101 aattcgccga tttagatcga ctcatgcaat taaaagagtc ctcaaattta accgtcagtc
     5161 ttgcgatgcc ggctttaaat gaagaaaaaa cagtcggaaa tgtcatttcg acaatcaaaa
     5221 ccgcattgat ggacaacgtt ccgttgattg atgagatgat tttggtcgac tcaaattcaa
     5281 cggatcatac acgtgaaatt gcggaagatt tggggattaa aacgtacatt catcaacagc
     5341 ttcttccgga atcaggcgct aacgaaggga aaggtgaagc tctatggaaa agccttttgg
     5401 tcacttcggg agatatcatc gcttggatcg ataccgacat tgtcaatatt cgccctcatt
     5461 ttgtata
//
LOCUS       DAOSMM010000089         5272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_15753_length_5272_cov_16.246118, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000089 DAOSMM010000000
VERSION     DAOSMM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15753_length_5272_cov_16.246118"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK898_09380"
     CDS             complement(<1..210)
                     /locus_tag="PK898_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU20283.1"
                     /translation="MTKRFGDLPVFDHLNLAFQKGQTTCLMGESGCGKTTLIHLMVGL
                     LKPEKGKITGIEGSRISMVFQENRLC"
     gene            complement(234..950)
                     /locus_tag="PK898_09385"
     CDS             complement(234..950)
                     /locus_tag="PK898_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU20284.1"
                     /translation="MFWLAVWQALSMIPGWNIFLASPIVVFKTAIQLVREPVFWTTVL
                     FSFTRIVAGFIFGLCAGIVFAILSVNFQIIRLLLEPIVLLIKSTPVASFIILVLFFIS
                     SSMLSTVIAFLMVFPILYTNTLQGILETDKQLLEMASVFKISKWKKFRYIYVHDVMPF
                     FISGVTVALGICWKSGIAAEVIGLPTGSIGEKLYEAKIFLSSGNLFAWTAVIILISFG
                     FEKFFLWALRKVQVKLESCA"
     gene            complement(982..1968)
                     /locus_tag="PK898_09390"
     CDS             complement(982..1968)
                     /locus_tag="PK898_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20285.1"
                     /translation="MKSNKLLIIIVSFLFAFFITVNTINFHVQSQDNVSETIRIGALK
                     GPTGMGLAKLMEDSQAGDEKQFTLTGTADEIVTGLVKGELDFGAVPCNLASVLYHKTN
                     GGIKVAAINALGVLYVLENGNTIHTVQDLKGKTIYTTGKGTTPEYVFRYILTKNSIDP
                     DKDVEIIFKSESTEIASMFASEKNLIALLPQPYVTTVQMQQPDVRIALDLTQEWEKVN
                     PDHPLATGCVVVRTELTENDPETVKSVLSEMADSVSYVNQNVDDAAALIAKFGIIPNA
                     EIAKKAIPYCNITFIHGEEMQKALSEYLTILFEANPVSVGEKLPDEAFYFKY"
     gene            2211..2645
                     /locus_tag="PK898_09395"
     CDS             2211..2645
                     /locus_tag="PK898_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU20286.1"
                     /translation="MKVEIKILEDIIEPYAIIYTSALTDEIQKAATLISIQENAVPVM
                     LEEKIFMLKPDEIFMARVENNSLAIYSESRRYLCKKRLYEISEILGAGFMQISKSTVV
                     NLSEVDYIEPFFNGMVNLKLKNGLSDYISRKYMKDFKRYFGL"
     gene            2658..3095
                     /locus_tag="PK898_09400"
     CDS             2658..3095
                     /locus_tag="PK898_09400"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HOU20287.1"
                     /translation="MIEIIKRIIEGIVIGCTIFTIIGIIFCLVNGTDTAFGDPDYGFV
                     KMAICSMVVGLGFSLPTFIYDDQKITKPLQTLIHLSIGFIIFGLVAFYAKWVPVESGI
                     KEIVVFIILSILSTLAIWYCFYLYYKNEARKINDDIRKKQNKN"
     gene            3287..3784
                     /gene="rplI"
                     /locus_tag="PK898_09405"
     CDS             3287..3784
                     /gene="rplI"
                     /locus_tag="PK898_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOU20288.1"
                     /translation="MKVLLLKDVYKLGHAGDVKKVADGYARNYLIPQGLVTMATPGAI
                     KQADQIKQKADAIRSQLNTEMSSLFEKIDGMVITFASKAGETGKLYGSITPQMIVDAI
                     NEKSGASIEKRQLEVEPIRTLGEHHVTVRLTVDIVPTVKVIVHREGEKVNLESLVTEE
                     PEKAE"
     gene            3855..5195
                     /locus_tag="PK898_09410"
     CDS             3855..5195
                     /locus_tag="PK898_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOU20289.1"
                     /translation="MSVSVGKALRSARESKNIPLSKVSDDLYIRITYLQAIENGRPEI
                     LPSPVQGRGFVRMYSDYLKLDTEKILYAWDHPDIPFSLDSEMSNQTESPAPQEPIIEK
                     TESSSQPSKTDTANSQNADPVSAIYKQIGSELRQRREILGLSYEDAEKHTLVRSIYLK
                     LMEDGNFDSLPSSVQARGMLNNYAAFLNLDVENIMLQYANALQLKSASRIAETAKEKK
                     NKKEPKPTKKAGKFRQFMTPDLFVGVSVLIAMFAIIIYSAVTISEYKAKAVQKTVDLN
                     LNFINQLATHDITVTATSDFNETPTVPISEMNPSEPAQMNTQSENLTVEETNRPIQVF
                     VEANQRAFMKVVSDGDVVFTGRTLPGNTYPFDASQMIEVTTGNAAALKITYNQQSLGL
                     MGKLGEVITLQFTNNAIMTPTPAITMTPTLTLEPTYTPQVFEATPTFTITPYIP"
BASE COUNT         1670 a         1106 c         1006 g         1490 t
ORIGIN      
        1 gcataatcga ttttcctgaa aaaccatcga tattctgctt ccctcgatcc cggttatctt
       61 tcccttttcg ggtttaagca aaccgaccat cagatggatc agggtcgttt ttccgcatcc
      121 gctttctccc atcagacaag tcgtctgacc tttctgaaaa gcaagattca aatgatcgaa
      181 gaccggtaaa tctccgaatc ttttcgtcaa attaattatc cggatttcat tatctatgcg
      241 caactttcta acttaacctg aacctttctc agtgcccata agaagaattt ttcaaatccg
      301 aagctgatta aaataattac cgctgtccag gcaaataaat tacccgaact aagaaatatt
      361 ttggcttcat aaagcttttc accgatgctg ccggtaggca atccgatcac ttccgcagcg
      421 ataccgcttt tccagcatat tccgagcgca accgttacac cgcttatgaa aaaaggcata
      481 acatcatgca cataaatata gcgaaatttt ttccatttgg aaattttgaa tacagatgcc
      541 atctctaata gttgtttatc cgtttccaat attccctgca aggtatttgt ataaagaatc
      601 gggaaaacca tcaagaacgc aattacggtc gaaagcatag aacttgaaat aaagaataaa
      661 accaaaataa tgaagctggc aaccggtgtc gatttaataa gcaatacaat cggttccaat
      721 aacaatcgga ttatttgaaa atttacagaa agaatcgcga atacaattcc cgcgcataag
      781 ccgaaaatga atcccgcaac aatccgtgta aagctgaata aaacagtggt ccagaaaaca
      841 ggttcccgga ccaactgaat agctgtttta aacacgacaa tcggagaagc cagaaaaata
      901 ttccaacccg gaatcatact aagtgcctgc caaacggcaa gccaaaacaa tataatccaa
      961 aatcgccgga atgtttttct ctcagtattt gaaatagaat gcttcatcgg gtagtttctc
     1021 gcccactgaa accggatttg cctcaaataa gattgttaaa tattcactca aagctttctg
     1081 catctcctcg ccatgaatga aagtaatatt gcagtaagga atagcctttt tggcaatttc
     1141 ggcattggga atgataccga attttgcaat caatgcagcc gcatcatcca cgttttgatt
     1201 gacataggac acggaatcag ccatttcaga taatacactc ttcaccgttt cgggatcatt
     1261 ttccgtcaat tcagttcgga ctaccacgca tcccgtagcc agcggatgat cgggatttac
     1321 tttctcccat tcttgggtca gatcaagagc tattcttaca tcgggttgtt gcatctgaac
     1381 agttgtgaca tagggttggg gtaataatgc gatcaaattt ttttcagatg caaacataga
     1441 agcgatttcg gtcgattcac ttttaaaaat gatctccaca tccttatccg gatcgatgct
     1501 gtttttcgtc aaaatgtatc gaaacacgta ttcgggtgtc gtcccttttc cggtggtata
     1561 aatcgttttt ccttttaaat cctgaacggt atgaatggta ttcccatttt ctaatacata
     1621 taaaacgcct aaagcattga ttgcggcaac tttaatcccg ccgttcgttt tatgataaag
     1681 aacgcttgcc aggttacagg gaacagctcc aaagtccagc tcccccttaa ccaaaccggt
     1741 aacaatttca tccgctgttc cggtcaaggt aaattgtttc tcgtcccctg cctgtgaatc
     1801 ttccattaac tttgccagac ccattcccgt cggtcccttt aaagcaccga tccggatcgt
     1861 ttcagaaaca ttatcttggg attgaacatg aaaattaatt gtattaacgg ttataaaaaa
     1921 agcaaaaaga aaactaacaa taattatcaa taatttattt gacttcattt taactcctga
     1981 aaatattgca ttttgcttat tttacctcat tcgtgaaaat attatcattt actatgattt
     2041 aaaaaaaggg gaacaaatat tatcaaaatc aaattttgat aatattcatt tataaaattt
     2101 ctttctcaga tgatttcttg catttcaacg catattttct gccacttacc gattttttgc
     2161 tgacgaatga atcgctatat gttaatattt gctcaaaagg agaaaattca ttgaaagtcg
     2221 aaatcaagat attagaagat attatcgagc catatgcaat tatatataca agtgccctga
     2281 ccgatgaaat ccaaaaagcg gcgactctga tttcaataca ggaaaatgcc gttcctgtta
     2341 tgctcgaaga aaaaatattt atgctcaaac cggatgaaat ctttatggca cgggtggaaa
     2401 ataattcgct ggcaatatac agtgaatcaa gaaggtatct ctgtaaaaaa agattatatg
     2461 agatttctga aattctcggc gccggtttta tgcagatttc aaaatcgacg gtcgtaaatc
     2521 tttcagaggt ggattatatc gaaccttttt tcaacgggat ggtgaatctc aagttgaaaa
     2581 acggtctatc cgactatatc tcaagaaaat atatgaaaga tttcaagaga tattttggtt
     2641 tataaaagga gaagataatg atagaaatta taaagcgaat catcgagggg atagtaatag
     2701 gctgcaccat ctttacaata atcgggatca ttttttgcct ggtcaacgga acggataccg
     2761 cattcggcga tcctgattat ggatttgtaa aaatggcaat atgttcaatg gttgtaggat
     2821 tgggtttttc tctcccaacc ttcatctatg atgatcaaaa aatcacaaaa ccgttacaaa
     2881 cgcttatcca tttgtcaatt ggttttatca ttttcggttt ggtggctttc tatgcaaaat
     2941 gggtaccggt cgagagcgga attaaggaaa tcgtcgtttt tatcatccta tcaattttgt
     3001 cgaccttggc gatttggtac tgcttctatt tgtattacaa aaatgaagcg agaaaaatta
     3061 atgacgatat ccgaaaaaaa caaaacaaaa attgaacaaa tttttgaacc tccttttcaa
     3121 tggatgacag aatcgccggc accttatcgg aatgatagtc ttgctgaata gcaacacaac
     3181 ccgtcatagc cacgattgac ccgtttccga ttatttggta gaatactgtt cgccgatgtt
     3241 gtacccttca tcggaaaata tatctaaaat aaggaagggc ttgaaaatga aagtattatt
     3301 gttaaaagat gtctataaac tcggacatgc gggtgatgtt aagaaagttg cggacggata
     3361 cgcgcgcaac tatttgattc cacaaggtct tgttacgatg gcgactcccg gtgccattaa
     3421 acaggcggat caaatcaaac aaaaagcgga tgcgattcgt tctcaactga ataccgaaat
     3481 gagttcttta ttcgaaaaga tcgacgggat ggttataact tttgccagca aagccggaga
     3541 aaccggcaaa ttatacggat caatcactcc gcaaatgatc gttgatgcga tcaatgagaa
     3601 atccggcgcc tcaatcgaaa agcggcagtt ggaagttgaa ccgattcgaa ccttgggaga
     3661 acaccatgtt accgttcgat tgactgttga tattgttccg accgttaaag tgattgttca
     3721 tcgtgagggt gaaaaggtca atctggaatc tcttgtgacc gaagaacctg aaaaagcaga
     3781 ataaattact attttatata caatcggaag aaggtcaggc atgtactggc ctttttcttt
     3841 tataattacc caatatgtct gtgtctgtcg gcaaagcgct acgatcggct cgcgagagca
     3901 agaatatccc actcagcaaa gtgtcggatg atctttatat ccggattact tatctccaag
     3961 ccatagaaaa cggacgtccg gaaattcttc cttcacccgt acagggtcgg ggatttgttc
     4021 ggatgtattc cgattattta aagttggata cggaaaaaat cctctatgct tgggatcatc
     4081 ccgatatacc gttttcactt gattctgaga tgtcgaatca aaccgaatcg cccgctcctc
     4141 aggaaccgat catagaaaaa acggaaagtt catcgcaacc gtccaaaacg gataccgcca
     4201 acagtcaaaa cgcggacccg gtttctgcta tttataaaca aatcggcagc gaactgcgac
     4261 aacgcagaga aatcctcggt ttatcctatg aagatgcaga gaaacataca cttgtccgca
     4321 gtatttatct taaattaatg gaggacggta atttcgattc tttgccttct tcggttcagg
     4381 cacgagggat gctgaataat tatgcagcct tccttaatct ggatgtcgaa aacatcatgc
     4441 tccaatatgc caatgcgctg caattaaagt ccgcttcaag aattgccgaa accgcaaaag
     4501 aaaaaaagaa taaaaaggag cctaaaccga cgaagaaagc cggaaaattc aggcaattca
     4561 tgacaccgga tctgtttgtc ggtgtatcgg ttctgatcgc gatgtttgca attatcattt
     4621 attccgctgt gacaatttcg gaatataaag ccaaagccgt tcagaaaact gtcgatttaa
     4681 atctgaattt tatcaatcaa ttggcaactc acgacatcac agtaaccgcg acgagcgatt
     4741 ttaatgaaac tcccaccgtt ccgataagcg aaatgaatcc gtcggaaccg gcacagatga
     4801 atacccaatc agaaaatttg actgtcgagg agacaaaccg gcctattcaa gtttttgtgg
     4861 aagcgaatca gagagcgttc atgaaagtcg tttccgatgg tgatgtggtg tttaccggca
     4921 gaactctgcc ggggaatacc tatccgtttg atgcaagtca aatgattgaa gttacgaccg
     4981 ggaatgccgc tgcgctaaaa ataacttaca accaacaatc tttaggtttg atggggaaat
     5041 taggtgaggt gataacactc cagttcacaa ataatgcaat tatgaccccc actcccgcga
     5101 tcaccatgac gccgaccctt acgttagaac cgacatacac acctcaggta ttcgaagcaa
     5161 caccgacttt taccattaca ccttacatcc cttagaattc aaggacttat gcaaaaaaac
     5221 acttttcatc taatttccct cggctgtgca aaaaacacgg tcgattccaa ta
//
LOCUS       DAOSMM010000090         5201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_16054_length_5201_cov_16.984065, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000090 DAOSMM010000000
VERSION     DAOSMM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16054_length_5201_cov_16.984065"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK898_09415"
     CDS             complement(<1..203)
                     /locus_tag="PK898_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU20290.1"
                     /translation="MTENQSKKKVIIIGAGPAGLTAAFELLRQEPESYNVTVLEESAA
                     IGGISRTVQYNGNRMDIGGHRFFS"
     gene            complement(324..2705)
                     /locus_tag="PK898_09420"
     CDS             complement(324..2705)
                     /locus_tag="PK898_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU20291.1"
                     /translation="MDLIGQKYRIIEECGEESENSFYRVVDETDGKHYYLDLIRTDNP
                     SLSAKGLIELQVRAQEHSRFRHSNAIPFYPPEVTDNGLILIQNDIPGKSLNYVMKTRG
                     RPLPQNQAYRITYAVVSALAAMHDLQMTHGELDSSHILINDSGDSFISFLPLPDDFNA
                     QNALYRFPETESTVKSKFSDDIYAMGVILTEMFTSLIPFGFAENKDGKKNTRAFEYYK
                     IGLAQISDESNEAIIPVILRCLNPDPKNRYENAVELFIDIRKIIEAWVNNTPGQENIL
                     VLPVIKKPIDQKEEDFENKIKKPSSSSKERVRSQRKSNKKKGSLLISFISAAIVITGG
                     IVLFFYLSEVADTKTSVTNYQGTLENLNNTQTALAYNPAIITPQVTKKSSSSIKKTAI
                     PTPLPTFTLTATATFTPVPIQRSIGAAIHWTADQSEMVFVPSGNFRMGMDATFGFAIE
                     NLFPVHEVDLDNYWIDRTEVTFEQYGRCVKAGACPASEAASPITADQKKPVTGISWEN
                     AATYCKWAGKRLPTEAEWEKAARGTDLRLYPWGNDSPQYTDQGNWFSTEIQNADEMNR
                     DISPYGAIHMSDNVSEWVNDYFSPTFTTATEYQNPQGPVSGAMRTVKGGAATNQDPEI
                     ASFVFNRWSADPIRPQDYGFRCAVSDSDQIQEKADGSEKPVPVSPLGTIPETGCENRI
                     GFVADITIPDGTPVHANEIITKTWRLKNIGTCTITKNYKIVWADTTFVNPQKLFDFNT
                     EILPGEEKEVSITFPVIGNGKTRINFKFAGLDGVPFQLGERGRGAFWIEYVIQ"
     gene            2999..3475
                     /locus_tag="PK898_09425"
     CDS             2999..3475
                     /locus_tag="PK898_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20292.1"
                     /translation="MADIEKIIKEVQDAIHSGSEKVTDSVTESDMFDKYKDSDDKIGD
                     ILEDAGNVVTGWFDAVSDKVEPVVKKTKAKIYEGIQEEYRKAGAPYGDTHEGLLRWVD
                     EHETNIREKVNTGLEKARKTILDTAKKTKDFVEEKITENQSASKTTVEGSTKKSEK"
     gene            complement(3612..3968)
                     /locus_tag="PK898_09430"
     CDS             complement(3612..3968)
                     /locus_tag="PK898_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20293.1"
                     /translation="MTAKSLYMNNFTFSLQNLTCILDAKSVAPDIERSNFGTVLDEKK
                     TVDEVFLFMPIEAAKELGISILQMVAAQEKRTHLKIRLEPEKQALWDCYVNAAEEFRQ
                     QNNEAKKADEKTTPSV"
     gene            complement(3965..4447)
                     /locus_tag="PK898_09435"
     CDS             complement(3965..4447)
                     /locus_tag="PK898_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOU20294.1"
                     /translation="MMKSNNLFRLNVGFLINQAVGSYRQFEIDAPFIQIDEDTRFNNV
                     KTDIRVSRVQQGILVSVEAGAVIDLECVRCLDPYVQTIDCNFDELYAFHIRQNIGAES
                     YLPENGFIDLLPLLEEYLTLEIPISPLCDENCKGLCDVCGANLNYETCIHHQESEIKS
                     "
     gene            4547..>5201
                     /locus_tag="PK898_09440"
     CDS             4547..>5201
                     /locus_tag="PK898_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20295.1"
                     /translation="MIRPPHDLSFPRKKTTVFILILSFLEGVAVFVHLISIPSESENA
                     FLLGFSKSRLLLIVSCVFLSIFYAFLLIRFLRKGFLLPDRRKNIWLYRILISIFWVIF
                     WGIFFFLLLSVLPVQKSDSTVSSIAFRIWDVLLWGALLSVQMIFLIQFQTDYWHEIFN
                     NYIKAFFFALFLIVFYLFISYYDRIDWIRKFKYSEITILTPAGIALIWAILSGKIKDR
                     "
BASE COUNT         1417 a         1043 c         1079 g         1662 t
ORIGIN      
        1 gagaaaaaac gatgtccccc gatatccata cgattgccgt tatattgaac ggttcgggaa
       61 atgccgccga tggcagcgga ttcttccaaa acggtaacgt tataagattc cggttcctgt
      121 ctgagaagtt caaatgcggc tgtcaatcct gccgggcctg cgccgataat aattactttt
      181 ttctttgatt ggttttctgt catatcgctt ccggatttat tttttgatct cgtcattgtt
      241 cgatcagtca ttcgacaatg cgattataac tgatctgagt cgttaaaaag gaatcggatc
      301 tcttgggaga atcaattcta tttttattgg atcacatatt cgatccagaa tgctccacga
      361 cctctctcac ccagttggaa cggaacaccg tccaacccgg caaatttaaa attaattcgg
      421 gttttcccgt taccgatgac ggggaaagtt atgcttactt ccttttcttc cccgggaaga
      481 atttcggtat tgaaatcaaa taatttttgg ggattcacaa aagttgtatc cgcccagaca
      541 attttgtaat tttttgtaat cgtacaggtg ccgatattct ttaaccgcca cgtctttgtg
      601 atgatttcat ttgcatgaac aggcgttccg tccggaattg ttatatcggc tacaaaaccg
      661 attcgatttt cacaaccggt ttccggaatt gttccgagag gtgaaaccgg gaccggcttt
      721 tccgaaccgt cagctttctc ttgaatttga tcggaatcac tgacagcaca ccggaatcca
      781 taatcctgtg gacggatcgg atcggcactc caacggttga aaacaaacga agcgatttca
      841 ggatcttgat tggtcgccgc ccctcctttg accgttcgca tagctccgct gaccggtcct
      901 tgcgggtttt gatattcggt tgccgttgtg aatgtcggac tgaaataatc atttacccat
      961 tcgctgacat tatcactcat atggattgct ccataagggc tgatatctcg attcatttca
     1021 tctgcatttt ggatttcggt ggagaaccaa tttccctgat cggtatattg agggctgtcg
     1081 tttccccaag gataaagcct taaatctgta ccgcgcgccg ctttttccca ttccgcctcg
     1141 gtcggaagac gcttcccggc ccatttacaa taagtcgccg cattttccca agaaataccg
     1201 gttacaggtt ttttttgatc ggctgttatc ggactggcag cttccgaagc cgggcacgct
     1261 cccgctttaa cgcatcggcc gtattgttca aatgttacct ccgttcgatc gatccaataa
     1321 ttgtccagat caacttcatg caccggaaaa agattttcga tcgcgaaccc gaaagtagca
     1381 tccataccca tccggaaatt ccccgaaggc acaaatacca tctcggattg atcggcagtc
     1441 caatgaatgg cggctccgat ggacctttga atcggaaccg gagtgaaagt cgcggtcgcc
     1501 gttaatgtaa atgtcggcag aggggtgggg atagctgtct tttttatgct cgaagaagat
     1561 tttttcgtca cttgaggagt aatgattgcg gggttataag ccagagctgt ctgtgtatta
     1621 tttaagtttt caagagttcc ctgatagttg gtcacggagg ttttggtatc tgccacttcc
     1681 gaaagataga agaacagaac aattccgccg gtaattacaa tcgcagctga aataaaagaa
     1741 atcaacaacg agcccttctt cttattgctt ttgcgctggg atcggactct ttctttggaa
     1801 gaagatgacg gtttttttat tttgttttca aaatcctctt ctttttgatc gatcggtttc
     1861 tttatgacag gcagcaccaa gatgttctcc tgtccgggag tattattcac ccacgcttcg
     1921 atgatttttc gaatatcaat gaacaactcc actgcatttt catatcgatt tttcggatcg
     1981 ggattcagac agcgtaaaat caccgggata attgcttcgt tcgattcatc gctgatttgc
     2041 gccagcccta ttttgtagta ttcaaatgcc cgggtattct ttttaccgtc tttattttca
     2101 gcaaaaccaa aggggattaa cgaagtaaac atttctgtaa gaatcacacc catcgcatag
     2161 atatcatcac tgaacttgga cttgacggtg gattccgtct cgggaaaacg atataaagca
     2221 ttttgggcgt taaaatcatc cggaagagga agaaatgata taaaagaatc accggaatca
     2281 tttatcaaaa tatgactgga atcaagttcc ccatgggtca tctgaagatc atgcatagct
     2341 gccaatgccg aaacaaccgc ataagtaata cgatatgcct gattttgtgg caaaggacgg
     2401 ccacgggttt tcataacata atttaatgat ttccccggaa tatcattttg gataagaatt
     2461 aatccgttgt ccgtaacttc aggaggataa aatggaatcg cattggaatg tctaaagcga
     2521 gaatgttcct gtgcgcgaac ctgtagttca attaatcctt ttgcactgag cgatgggttg
     2581 tccgttcgaa ttaaatccag atagtagtgt tttccatctg tctcgtctac aactcgataa
     2641 aaggaatttt cagattcttc gccacattct tcaatgattc ggtatttttg tccgatgaga
     2701 tccatatcaa atccgattgt cgataatttt cattatcact ttgaatcaaa ttatagttta
     2761 ttgaacgcga ttaatagcat tcgttttgtt gttttgtaag atagaaaatt attgaataat
     2821 caaatcgtta aaaagcatgt gagaggttta ttttgattga accgaatggt tcaaaagttc
     2881 tgcttttttg gaccaatcat atctcgagac attttctcat agatgcgcta tcatatactt
     2941 tgaaactatt cggatattat ttcgtatacg ttttatcgga atttataaag aggaaatcat
     3001 ggcggatatt gagaaaatta ttaaagaagt tcaggatgcc attcattcgg gttctgaaaa
     3061 agtcaccgat tcggtaacgg agagcgatat gtttgataag tataaagatt cagatgataa
     3121 gatcggggat atcttagaag atgcggggaa tgtcgtcacc ggttggttcg atgccgtatc
     3181 ggataaagtg gaaccggttg tgaagaagac caaggcaaaa atttatgaag gtattcagga
     3241 agaataccga aaagccggtg cgccatatgg tgatacacat gaaggtttgc tgcgttgggt
     3301 ggatgaacat gaaaccaaca tccgtgaaaa agtaaatacc gggcttgaaa aggcacgtaa
     3361 aactattctt gataccgcaa agaaaacaaa agattttgtg gaagaaaaga tcaccgaaaa
     3421 tcagtccgca tctaagacca cggtggaagg aagcacaaaa aaatcggaaa aatgattctg
     3481 atttatttta aacttagcgg aaatggatag gttttaaaca agattctcaa ttattcaata
     3541 ggcgatctca ttccggaaaa ttcactccgg tgagatcgct tttttatttc gttttctgaa
     3601 gcttcattga tttaaacaga gggagtggtt ttttcatccg cttttttggc ctcattgttc
     3661 tgttgccgga actcttcggc tgcgtttaca tagcaatccc agagagcttg tttttccggt
     3721 tcaagcctga tttttaaatg tgtccgtttt tcttgtgctg cgaccatttg caaaatcgaa
     3781 atccccagtt cttttgctgc ttcgatcggc atgaataaga atacttcatc caccgttttc
     3841 ttttcatcca gtaccgtacc gaaattggat cgttcgatgt cgggtgcgac tgattttgcg
     3901 tccaaaatac aagtcagatt ttgaagactg aacgtaaagt tattcatata cagacttttt
     3961 gcagtcatga tttaatctcc gattcttgat gatggataca agtctcgtag ttgagattcg
     4021 caccgcaaac atcacaaagc cctttacaat tttcatcgca aagaggactg atggggattt
     4081 cgagggtgag atattcctct aaaaggggta aaagatcgat aaatccattc tctggtaaat
     4141 aggattccgc accgatattt tgccgaatat gaaatgcata aagttcatca aaattacaat
     4201 cgatggtttg tacgtacgga tccagacatc ggacgcattc caaatctatt acggctccgg
     4261 cttccacgga aaccagaatt ccctgctgta cacgactgac acggatatct gtttttacat
     4321 tgttaaatcg ggtatcctca tcgatttgaa tgaaaggtgc atcaatttca aactgccggt
     4381 aagagcctac cgcttggtta attaaaaaac cgacatttaa gcggaaaaga ttatttgatt
     4441 tcatcataat atttttccaa ttttcatgct gattatatcg catatcgaaa atcgttctcc
     4501 gcctcgttac gagaatttca ataggaagcg tgatataaat atccgtatga ttcgcccacc
     4561 tcacgattta tcattccccc gaaaaaaaac caccgttttt atcctgattc tttccttttt
     4621 ggaaggggtt gcggtatttg ttcatttgat ttcaattcca tccgaatccg aaaacgcttt
     4681 ccttttggga ttttcaaaaa gtcgtttgct cctgattgtg agttgtgttt ttttaagcat
     4741 cttttatgca tttctgttaa tccgattcct gcgaaaggga tttttgcttc ccgaccgtcg
     4801 caagaatatt tggttgtatc gaattttgat ttcaattttt tgggtaatct tctggggtat
     4861 ttttttcttt ttactgttgt ctgttttgcc tgttcaaaaa tcggattcca ctgtatcatc
     4921 gattgctttc agaatttggg atgtactttt atggggggca ctgctttccg tacagatgat
     4981 ttttctgatt caattccaga cggattattg gcatgaaata tttaataatt atattaaagc
     5041 gtttttcttt gcgctttttt tgattgtttt ttatttgttt atttcatatt atgatcgaat
     5101 tgattggatt cgaaaattca agtattcgga aataaccatt cttacaccgg caggtatcgc
     5161 tttgatttgg gcaatactgt ccggaaagat caaagatcgt a
//
LOCUS       DAOSMM010000091         5159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_16234_length_5159_cov_15.860502, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000091 DAOSMM010000000
VERSION     DAOSMM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16234_length_5159_cov_15.860502"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="eno"
                     /locus_tag="PK898_09445"
     CDS             complement(<1..1045)
                     /gene="eno"
                     /locus_tag="PK898_09445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU20296.1"
                     /translation="MESIIEQVYGREILDSRGNPTVEVEVVLMDGNYGRAAVPSGAST
                     GIHEALELRDGDKKRYLGKGTQKAVENVNEIIADEIIGMDATEQMEIDQALIDLDGTP
                     NKSKLGANAILGTSLAVAKAAANSLSLPLYRYIGGVWAHTLPVPMMNILNGGAHTGWQ
                     STDAQEFMVMPFGAESFAEGLRWGAEIYHSLKSVLKSRGYTTLVGDEGGYAPALKANV
                     EAVEVILEAIEKAGYKAGVDVAIALDPAASELYDEETKKYNLRKEGKMLSSEEMVAFW
                     KSWVDQYPIVSIEDGLAQDDWDGWKMLVAELGDRLQIVGDDLLVTNPERVRRAIKENA
                     ANALLVKFNQIGSL"
     gene            1366..2568
                     /locus_tag="PK898_09450"
     CDS             1366..2568
                     /locus_tag="PK898_09450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU20297.1"
                     /translation="MSNHQICYNCFSGTNGINPCPYCGFDNSSVEKKQHLLTPGTILH
                     EGYLIGRPLGQGGFGITYLGYDLASHSRVAIKEYFPAGIAVRQNGIVQAASDENQAIF
                     KKGVETFNKEAQILSHLGSQPNIVNILAFFFENGTGYFVMEFLEGRSLKDYLNSRGGK
                     LSFQETVSLLLPVMKALSAVHHEGMLHRDIAPDNIYVTDDGQVKLMDFGAARYHTFLE
                     TQSIRTIVKPGYAPMEQYTSQSAQGPWTDVYAMGATIYRTLTGIVPPDAPSRYMKDEI
                     KSPLQLGCILPNNADYAIMRALSPQIVFRFRTMDDFAGALTMPDPPVPLQAMPINPMK
                     LNQPVQPSAYSEGNPSDSDENATRMIQINLNPTKPEKKDIPYWLFFVLIFFLVGLIIW
                     FIISLSSI"
     gene            2598..3572
                     /locus_tag="PK898_09455"
     CDS             2598..3572
                     /locus_tag="PK898_09455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU20298.1"
                     /translation="MKITDPFPDSICPFCFTEKKSETICPCCGCGEDSLPQFVAQQIL
                     PGSVLNSRFVVGKLLGQGSFGITYLCFDSERNQKVAIKEYFPSEYVRRQNLEMLPLAD
                     EYSDTVIRGMEYFCEEVKILMEFNQHSNIVQISDYFFENGTAYFVMEYVEGASLKNYL
                     DVQGDRISFEESWEILLPIMKTLAEVHEAGLLHRDIAPDNIFLCKNGEPKLLDFGSSR
                     FAFSRDMTEFPLTIKPGYAPPEQYRDFDRQGPWTDIYQMGAVFYRAITGRIPPEAIER
                     SVSDPLTSPLQLGLNIPEEADRSIMKALNLDPRDRFRNFFEMIKGFQC"
     gene            3857..4861
                     /locus_tag="PK898_09460"
     CDS             3857..4861
                     /locus_tag="PK898_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOU20299.1"
                     /translation="MLELLLPLNIVLIIIILLVSLFLLSKSADFLVDNAVKLSKILKI
                     PEMVIGATIVSFGTTLPEFSASTVSALRGNFGFALGNAIGSIITNTSLVLGVGALFGA
                     IPVTRKASQKLSILNIVVLLLILPTIPRVLRNGEGTIPQWMGVVLLVLVPFYVLFLIA
                     QEKRSPEIEISDPEEAESHSNLFLTALLVLGAAAFVAFCASVLVASAEIIATRIGISD
                     ILISATIVAFGTSVPELTTAVSAAKTGHGGLAIGNVMGANILNILWVTGASIAVIPGG
                     ITVPNSFYLVHFPAMIAVLAIFGYFAYNKNRNEINHKEGILLTGIFGIYLLANVIFTL
                     "
     gene            4886..>5159
                     /locus_tag="PK898_09465"
     CDS             4886..>5159
                     /locus_tag="PK898_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU20300.1"
                     /translation="MKTRRKILLTNTQIIALGFFLVILAGSILLMLPISSKNGSWTPF
                     IDALFTATSATCVTGLVVFDTFTHWTVFGQIVILTLIQVGGIGLMTI"
BASE COUNT         1347 a         1134 c         1161 g         1517 t
ORIGIN      
        1 tcagggaacc gatttgattg aatttcacca agagggcatt cgccgcattt tccttgatcg
       61 cccggcgaac acgttccgga tttgtaacca gtaaatcatc gcctacgatc tgaagtcggt
      121 cgcccaattc ggcgacgagc attttccaac cgtcccaatc atcctgcgcc aatccgtctt
      181 cgatggaaac gatcggatat tgatctaccc atgatttcca aaaagcgacc atttcttcgc
      241 tggataacat ttttccttct ttgcgaaggt tgtatttttt tgtctcttca tcataaagct
      301 cggatgccgc cggatcgaga gcaatggcaa catccacacc cgctttatag ccggcttttt
      361 cgatggcttc caaaatcact tcaaccgctt ccacatttgc tttcaatgcc ggagcataac
      421 cgccttcatc accgacaaga gttgtatatc cgcggctttt gaggacggat ttcaacgaat
      481 gatagatttc ggcaccccaa cgcagccctt cggcgaatga ctcggcgcca aacggcataa
      541 ccatgaattc ctgtgcatcc gtcgactgcc agccggtgtg tgctccgccg tttagaatat
      601 tcatcatcgg gaccggtaag gtatgagccc acacaccacc aatatagcga tacagcggga
      661 gactcagaga attcgcagct gctttggcaa ccgccagaga agttcccaga atcgcatttg
      721 cacccaactt ggatttattg ggagtgccgt ccaaatcgat caatgcctga tcgatttcca
      781 tctgctcggt agcatccatg ccgataattt cgtcggcaat tatctcgttg acattttcaa
      841 ccgctttttg ggttcctttt ccaagatagc gcttcttatc accgtcgcgg agttccagcg
      901 cttcatgaat accggttgat gcacccgagg gtactgccgc acgaccgtaa ttaccatcca
      961 ttaataccac ttcaacttca acggtaggat taccacgtga atccagtatt tcgcgcccat
     1021 aaacctgttc aattatgctt tccatcattc ttatcctttt ctgatattta aagggttatc
     1081 cctaatagtt tgagaatatt atatcaaatc cggactttta cggctttttt tgtcataatc
     1141 ggataattta aatcgagaaa aacaggtcct ccctgagaat ttctaaaaaa atgacattgg
     1201 ggtcaagcaa aaagccggtc ggttttgtat ttcgtttttg aatgggatga ggaacaacga
     1261 aacgaataac cctgtaatac gaaaagcggt tttatcgacg ccatcggcac gatgataaaa
     1321 ttatcttgat aaaagaataa ttgccatagt attccgaggc cttttttgtc gaatcatcaa
     1381 atttgttata actgtttttc aggaacgaac ggaatcaatc cctgccccta ttgcggattt
     1441 gataacagtt ccgtcgaaaa aaagcaacat cttcttacgc ccggaacgat tcttcacgag
     1501 ggatacctga tcggaagacc tttgggacaa gggggatttg gtattaccta tttgggatat
     1561 gatttggctt ctcattcccg agtggctatc aaagaatatt ttcctgccgg tatcgcggta
     1621 cgccaaaatg gaatcgtaca ggcggcgtcc gatgagaatc aagcgatctt taaaaaaggg
     1681 gtcgaaacct ttaataaaga agctcaaatc ctttcacatt tgggcagcca acccaacatt
     1741 gtcaatattt tggcgttctt ttttgaaaac ggtaccggtt attttgtaat ggaattcttg
     1801 gaagggcgat cgcttaaaga ttatctgaac agtcgaggcg gaaaactgag tttccaagag
     1861 acggtttctt tattgttgcc ggtgatgaaa gctttgagcg cggttcatca tgaagggatg
     1921 cttcatcggg atatcgcgcc ggataatatc tatgtcactg acgacgggca agtaaagtta
     1981 atggatttcg gcgcagcccg ttaccataca ttcttggaaa cacaaagtat ccgtacgatc
     2041 gtaaaacccg gttatgctcc catggagcaa tatacaagtc aatctgcaca aggtccgtgg
     2101 acggatgttt atgcgatggg tgcaacgatt tatcgaacgc ttacgggtat cgtccctccc
     2161 gatgcccctt ctcgatatat gaaagatgaa ataaagtcac cgctgcaatt gggctgtatc
     2221 ttaccgaata acgcagatta cgctattatg cgcgctttat ctcctcaaat tgttttccga
     2281 tttcgaacaa tggatgattt tgccggagcg ctgaccatgc ccgatccgcc ggtccctctc
     2341 caagccatgc cgataaatcc gatgaaacta aaccaaccgg tgcagccttc ggcttattcg
     2401 gaagggaatc cgtccgattc ggatgaaaat gccacccgga tgattcaaat caatctcaat
     2461 ccaaccaaac ccgaaaaaaa ggatatccct tattggttgt tttttgtgct gatctttttt
     2521 ttggtcgggc taatcatttg gttcattatc agcctttcca gtatttaggg agaaaaagat
     2581 tgccggccaa ttcctttatg aagattactg atccattccc cgactcgatc tgcccgtttt
     2641 gttttaccga gaaaaaatcg gaaaccatct gcccgtgttg cggctgcggt gaagatagtt
     2701 tgccgcaatt tgtcgcccag cagatccttc cgggttccgt tttgaacagc cgctttgtcg
     2761 tcggaaagct cttgggacag ggaagtttcg gaatcaccta tttatgtttt gattcggaga
     2821 gaaatcaaaa agtggccatt aaggagtatt ttccgtccga atatgttcgg cggcaaaacc
     2881 ttgaaatgct tccgttggcg gacgaatatt cggatacggt gattcgcgga atggagtatt
     2941 tctgcgaaga agtaaaaatt ttgatggagt ttaatcaaca ttccaatatt gttcaaatca
     3001 gcgattactt ctttgaaaat ggaaccgcat attttgtgat ggagtatgtc gaaggggcgt
     3061 ctttgaaaaa ttatttggat gttcaaggcg accgtatttc ttttgaagaa agctgggaga
     3121 tcttattgcc gattatgaaa accttggcgg aagttcatga agccggactc cttcatcgcg
     3181 acattgctcc ggataatatc ttcctgtgca agaatggcga gcctaaattg cttgatttcg
     3241 gatcttcgcg attcgcattc agccgggaca tgacggaatt tccgttaacg attaaacccg
     3301 gttatgctcc tccggaacaa tatcgtgatt tcgatcggca aggaccttgg acggatattt
     3361 accaaatggg tgcggtcttt taccgggcga ttaccggtag aataccgccc gaagcgatcg
     3421 aacgttcggt atcggatcct ttgacttctc cgctccaact gggattgaat attcccgaag
     3481 aagcggaccg ttccattatg aaggcgctta accttgatcc gcgagatcga ttccgaaatt
     3541 ttttcgaaat gatcaaaggg tttcaatgtt aaaatcaaaa atcgtcgata ccttccgaaa
     3601 atctaaaaaa ttatgataaa atagaccgag ccggatgaaa gcggtcgaac agattccgtc
     3661 tctgaaatca gtcaagagaa cgaaataatt ttttgaccga aatataaaaa tattatagat
     3721 tttacggtta ccgtgcagca atgacggtaa gattcggact ttttgggtgg gaagtctttt
     3781 tataaaggct tcccattttt ttattccgga aacggagtgg aagcggaaaa aattcactga
     3841 caagggaggt ttacaaatgc ttgaattatt attgccgctc aatattgtct taattattat
     3901 tattttattg gtatccttat ttcttttatc caaatccgcg gattttctgg tagataatgc
     3961 ggtgaaactt tcaaaaattt taaaaattcc ggaaatggtt atcggtgcca cgatcgtttc
     4021 ttttgggacc accttgccgg aattttccgc ttccaccgtt tcggcgttgc gggggaattt
     4081 cggatttgcg cttggaaacg cgatcggttc cattattact aacaccagtt tggttttggg
     4141 tgtcggcgcc ctgttcggag caatcccggt aactcgtaaa gcttcgcaaa aattaagtat
     4201 tttgaatatt gtagtcttgc ttttgatttt gccgacgatt ccccgagtgc tccgtaacgg
     4261 cgaagggacg attcctcaat ggatgggggt ggtcttgttg gttttggtcc ccttttatgt
     4321 gctgttttta attgcacagg aaaaaaggtc tccggaaatt gagatttcgg atcctgaaga
     4381 agcggaatct cattccaatt tattcttgac cgccctgctt gttttgggag cggctgcgtt
     4441 cgttgcattt tgtgcttcgg ttctggtagc ttccgccgaa atcatcgcga cgagaatagg
     4501 tatatcggat atccttatct cggcgacgat tgtagctttc gggaccagtg tgcctgaact
     4561 gacaaccgcc gtatcagccg ctaaaacagg tcatggcgga ctggcgatcg ggaatgtgat
     4621 gggagccaat attctgaata ttctgtgggt aaccggagca tcgattgcgg tgattccggg
     4681 cggaataacg gttccaaata gtttttatct ggttcacttt ccggcaatga ttgcggtgtt
     4741 ggcgatattc ggttattttg cctacaataa aaaccgaaac gaaatcaacc ataaagaggg
     4801 aattttatta accggaatct ttggaattta cttattggct aatgtcatct tcacactgta
     4861 ataaatccaa atataattag tgtgaatgaa aacgcgcaga aaaattcttc tgacgaatac
     4921 ccaaattatt gctttaggtt ttttcctggt aatcttagcg ggcagtatat tattaatgct
     4981 cccgatttct tccaaaaacg gatcatggac gccttttatt gatgcgctct tcaccgcgac
     5041 aagcgccacc tgtgtgacgg gtttggtcgt ttttgatact ttcacacatt ggacggtttt
     5101 cggccaaatt gtgatcctga cgctgattca ggtcggaggg atcggcttga tgacgattg
//
LOCUS       DAOSMM010000092         5145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_16302_length_5145_cov_16.547741, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000092 DAOSMM010000000
VERSION     DAOSMM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16302_length_5145_cov_16.547741"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK898_09470"
     CDS             <1..486
                     /locus_tag="PK898_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20301.1"
                     /translation="CLTTDPFMVGYPEVRQLSLASIGMINQNGIPCGILTKGVLPPEL
                     AEMDQRNSYGISLVSLDEEFRERWEPGAAPYRERIDALRYLHNKGMHTWVHMEPYPTP
                     NVISQDLNEILEEISFVDSIDFGRWNYSSLISHFPDSDAFYRAMEEQVRTFCEAHGIE
                     Y"
     gene            657..1610
                     /locus_tag="PK898_09475"
     CDS             657..1610
                     /locus_tag="PK898_09475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU20302.1"
                     /translation="MDQPEISVIIPFLNEEENLPDLIAELEAFAPTMPVRMEVVFVDD
                     GSTDGSLDLLRKVHFRNFSAKIVKLSRNFGSHPAVRAGFLNAAAPNCVWLGADLQEPL
                     EIIRLGYQKIREGYDLVCVQKKNVKVGKSEKLFSNTYSRLIRKYAIPGYPPDGVNTVF
                     FNEKVKRELNRNIESNSSVVLQVLNLGFKQTTLDVEYRERVHGRSKWSFGKKMKLMID
                     SFVAFSFFPIRMVSAMGFIFAIIGAIIALYLIIIKLFNIREVTLGWPSLISILMIGFG
                     ITNISLGVIAEYLWRTLDAARGRPSFIIESVEGLSQANQND"
     gene            1603..2619
                     /locus_tag="PK898_09480"
     CDS             1603..2619
                     /locus_tag="PK898_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU20303.1"
                     /translation="MINIVQIGYGYWGSNVARNISLSKKTNLAALVDKDPNRLEAAEN
                     TFHDRVELSTDYLPFLNDPEIDAFALAIQTEPSFEIAKQIMQAGKHLFIEKPMAENTE
                     KAIILNRIAKEKKVVLHVDHIMLFHPIIRRIKQMYDEGELGDLIYFDISRMNLGPIRR
                     DVNAMLDLAVHDLAVIDFISGGKEPYHVEAIGEKHYGNQETLTYLTLKYPGFLGHIKS
                     SWISPIKERRTIIGGTRKMVIFDDMKTIDKLTIYDQGIIEVGTEYGSYEFKARSGDIT
                     IPYIAQEDALRNSIEHFADCVENGTESIANGDHAIKIIRILDEAREKMKINSTEPKPE
                     NEPG"
     gene            2638..3900
                     /locus_tag="PK898_09485"
     CDS             2638..3900
                     /locus_tag="PK898_09485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU20304.1"
                     /translation="MKNIPIMKPYFDEDEAKEAAAALASGWVAQGPRVAEFEKAVAAY
                     EGNAYGVAVSSCTAALHLGMIALGFGSGDDVLVPSYTFVATANAVEYTGATAVPVEVD
                     IRTYTIDVGWAEKFIQSRYAEKEGRWINRKSGNRLAGIIPVNEFGLCADIPAVNALAK
                     RYGLKVFEDSACALGAKIGETHEGCFGNPSAVSFHPRKSITTGEGGMILTSDVELMEH
                     VRKLRSHAASLSEIQRHQNKGYLLPDYDELGYNYRMTDIQAGIGLAQMRKIELIMNRR
                     KAIAARYDEFLPQVVPFLKTPFVPEGYTHIYQTYACMIDTERLGMKSIEEGNRFRNEL
                     MDKLEQNGIATRQGTHAVHSLGYYQMKYGFRNMDLPVAYACDRLSIALPLYVELNEED
                     QDYVIDKLAELGKELMNSLKSQTRSVEK"
     gene            3909..4880
                     /locus_tag="PK898_09490"
     CDS             3909..4880
                     /locus_tag="PK898_09490"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU20305.1"
                     /translation="MPQIKGKNVLITGGAGFIGSHLTDALLKNQAGKVVIVDNFFLGK
                     MENLDFAQKNYNNLVIYRDDARDLGTMKAIIKKESVEVVFNLATIALNYSFFNPFGAY
                     RVNVDIAEVLLHLLQDGAYQTLIHSSSSEAYGTAVYSPMDENHPMNPTTPYAAGKASA
                     DLMIQSFYNVLHLDVAIIRPFNNFGPRQNDLALAAIIPQNARRILNGEKPVLEGTGEQ
                     TRDFIYVEDTARAFIMAYESEMTRGRVINLGSGKEISMKAVIDAICKYYGYNGGIEYR
                     PERPADVKRLCAGSELARELIGFTPQTDFETGLRKTLDWYQEKYTNL"
     gene            4877..>5145
                     /locus_tag="PK898_09495"
     CDS             4877..>5145
                     /locus_tag="PK898_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU20306.1"
                     /translation="MILCVFSDIHGNRLYFDFLADVWKGMDIDRFFFLGDAIGYFPDG
                     NEVLDRLKEFNPVCVKGNHEAMLLGLLPLSDEKDEVYHLKEQKANL"
BASE COUNT         1419 a         1016 c         1313 g         1397 t
ORIGIN      
        1 tgtctgacga cggatccttt catggtcgga tatccggaag tccggcagct ttcgttagcg
       61 tcaattggta tgatcaatca aaacggtatt ccgtgcggca tattgacaaa gggagtgtta
      121 ccgcccgaac ttgcggaaat ggatcaacga aattcttacg gaatttctct ggtttcattg
      181 gatgaagaat ttcgcgaaag atgggaaccc ggtgcggcac cctatcggga aagaatcgat
      241 gctttacgtt atctccataa caaaggaatg catacttggg tacacatgga accttacccg
      301 actcccaatg ttatttcaca ggatttgaat gaaattttgg aagaaatcag ttttgttgat
      361 tcgatcgatt tcggccgttg gaattattcg tcgttgattt ctcatttccc cgattcggat
      421 gcattttatc gggctatgga ggagcaggtc cgaacttttt gcgaagcaca cggaatcgaa
      481 tattgatcac ggtacacggt atccgtaatt gttcccgaac cgatttcgtt ttttacgggc
      541 gggaaggcgg agaaaatgag tgaataaaga cgaaataaag cgaagttttg atgcaaattc
      601 tccctatgat aaaataagag acgatatcgt aactgcaaga gcccgaagga aaatgcatgg
      661 atcaacctga aatttccgtt attatcccat ttttaaacga agaagaaaat ttacccgatt
      721 tgattgccga attggaagcg tttgctccga ctatgcccgt taggatggaa gtcgttttcg
      781 tcgatgatgg ctcgaccgac ggctctttgg atcttttaag aaaggtacac ttccggaatt
      841 tcagcgcaaa gattgtcaaa ctctcacgaa atttcggttc gcatccggcg gttcgagccg
      901 gtttcctgaa tgccgccgca ccaaactgcg tctggctcgg tgccgattta caggaaccgt
      961 tggaaattat ccgattggga tatcaaaaaa tccgggaagg gtacgatctg gtctgcgtac
     1021 agaaaaagaa tgtcaaagtc ggcaaatcgg agaaattatt ttcgaatacc tattcccggc
     1081 tgattcgtaa gtatgcgatt ccgggttatc ctccggatgg ggttaatacc gtttttttta
     1141 atgaaaaagt caaacgggaa ttgaatcgga atatcgaatc caattcgtct gtggtattgc
     1201 aggttttgaa tctgggattt aaacaaacga ctttggatgt cgagtatcgg gaacgggttc
     1261 atgggcgatc gaaatggtct ttcgggaaaa aaatgaagct gatgatcgat tcgtttgtcg
     1321 ctttttcatt ttttcccatc cgcatggtaa gtgcaatggg atttattttt gcgattatcg
     1381 gagcgattat cgcactttat ttaattatca tcaaactttt caacattcgg gaagttactc
     1441 tcggctggcc ttctttaatc agtatattga tgatcggttt cggcattacc aatatctcct
     1501 tgggcgttat cgccgaatat ctttggcgga cgttggacgc tgcccgaggc aggccatcgt
     1561 tcattatcga gtcggttgaa gggttgagtc aggcaaatca aaatgattaa tatcgttcaa
     1621 atcggttacg gttattgggg ttcgaatgtt gcacggaata tctctctttc caaaaaaacg
     1681 aatttggcgg ccttggtgga taaagatccg aacaggttgg aagcggctga aaatacattc
     1741 cacgatcggg tggaattaag cacggattat cttccatttc tcaacgatcc cgaaatcgat
     1801 gcttttgctt tggctattca gaccgaaccg tcctttgaaa tagcaaaaca gattatgcaa
     1861 gcgggaaagc atctgtttat tgaaaaaccg atggcggaaa ataccgagaa agcgattatt
     1921 ctgaatcgaa tcgctaaaga gaaaaaagtc gttctgcatg tagatcacat catgcttttt
     1981 catccgatta ttcgtcgcat taaacaaatg tatgacgagg gggaactcgg cgatctgatt
     2041 tattttgata tctcccgtat gaatctgggg ccgattcgcc gggatgtgaa tgctatgttg
     2101 gatttggctg ttcatgatct ggctgtaatt gattttattt ccggcgggaa ggaaccttac
     2161 catgtcgagg caatcggtga aaaacattat ggaaatcaag agacgttgac ctatctgact
     2221 ttaaagtatc cgggtttcct cggccatatt aaatccagtt ggatttcacc gattaaagag
     2281 cgtcgtacta tcatcggcgg tacacgaaaa atggttattt ttgatgatat gaagaccatc
     2341 gataagctga cgatttatga tcaagggatc attgaagtgg gcacggaata cggttcttat
     2401 gagttcaaag cgcgatcggg agatattacc attccttata tcgcacagga agatgctttg
     2461 cggaacagta ttgagcattt tgccgactgt gtggaaaacg ggaccgaatc gatcgccaac
     2521 ggagaccatg ccattaaaat tatccgtata ttggacgaag cgcgtgaaaa aatgaaaata
     2581 aattcaaccg agccgaagcc ggaaaatgag cccggttaaa ccggcaggaa gagaaaaatg
     2641 aaaaatatcc cgattatgaa gccttatttt gatgaggatg aagccaagga agctgccgcg
     2701 gcattggctt ccggatgggt ggctcagggt ccgcgcgtgg cggaattcga aaaagcggtg
     2761 gcggcttacg aaggaaatgc ctacggtgtc gcggtttcaa gttgcactgc cgccctgcat
     2821 ctcggcatga tagcgttggg gttcggctcc ggagacgacg tgttggtccc ttcctatact
     2881 tttgtggcaa ccgccaatgc cgttgaatat acgggagcga ctgccgtgcc ggtcgaagtg
     2941 gatattcgaa cctataccat cgatgtaggt tgggcggaga agtttattca atcccggtat
     3001 gcagaaaagg aaggtcgttg gatcaatcgg aaaagcggga atcgtttggc gggaattata
     3061 ccggtcaatg agttcggact ttgtgcggat attccggcgg tcaatgcact ggcaaaacgc
     3121 tatggcttaa aagtatttga agacagcgcc tgtgccttgg gtgcgaagat cggagaaacc
     3181 catgagggat gcttcgggaa tccctcggcg gtcagttttc atccgcgaaa gtcgatcacc
     3241 accggtgaag gggggatgat attaacttcc gatgtcgagt tgatggagca tgtgcgaaaa
     3301 cttcgttctc atgccgcatc gctctccgag attcaacgcc atcaaaataa gggatattta
     3361 ctgccggatt atgatgagct gggctataac tatcggatga ccgacattca ggcggggatc
     3421 ggcttagccc aaatgcgcaa aatcgaattg attatgaatc gccgaaaggc aatcgccgcc
     3481 cgctatgacg aattccttcc gcaggttgtt ccttttttga aaacaccgtt tgtaccggaa
     3541 ggatataccc atatttatca aacctatgcc tgcatgatcg atactgaacg actgggaatg
     3601 aaaagcatcg aagaaggaaa ccgcttccga aacgagctga tggacaaact ggaacagaac
     3661 ggcattgcga cccgacaagg gacgcatgcc gtgcactcgt tgggctacta tcaaatgaaa
     3721 tacggatttc ggaatatgga tttgccggtt gcatatgcct gcgatcgctt aagtatcgca
     3781 ttgccgttat atgtcgaact gaatgaagaa gatcaggatt atgtgattga caaattggcg
     3841 gaactgggaa aagaactcat gaattcgctg aaaagccaaa cccggtccgt cgagaaatag
     3901 aggaatcgat gccgcaaatc aaaggaaaaa atgttttgat taccggaggc gccggcttta
     3961 tcggcagcca cttgacggat gcattattga aaaatcaagc aggaaaagta gtcattgtcg
     4021 ataatttttt cttaggaaaa atggaaaatc tcgattttgc acagaaaaat tacaacaatc
     4081 tggtcattta tcgcgatgat gcccgtgatt tggggactat gaaggcaatc atcaaaaagg
     4141 aatccgtcga agtagtcttc aatttagcca ccattgcgct gaattattct tttttcaatc
     4201 ctttcggcgc ttatcgggtg aatgtggata tcgcggaggt tttgctgcac cttttacagg
     4261 acggggctta ccaaacactc attcattcgt cttcttcgga agcttacggg acggctgtct
     4321 attctccgat ggatgaaaat catccgatga atccgaccac gccgtacgcg gccggtaaag
     4381 cctcggcgga tttgatgatt cagtccttct ataatgtgct tcatttggat gttgcgatta
     4441 ttcgaccgtt taataatttc ggtcccagac aaaatgatct ggcattggcg gcgattatcc
     4501 ctcagaatgc cagaaggata ttgaacgggg aaaagccggt ccttgaggga acgggggaac
     4561 agacacggga tttcatttat gtggaagata ccgcgagagc ctttattatg gcttatgaaa
     4621 gcgagatgac acgcggcaga gtgatcaatc tcggttccgg aaaagaaatt tccatgaaag
     4681 ccgtgatcga tgcaatttgt aagtactatg gttataacgg cggtatcgaa tatcgtccgg
     4741 aacggccggc ggatgtgaag agactgtgtg cgggttccga actggctcgt gaattaatcg
     4801 ggtttacacc gcagacggat tttgaaaccg gcttgcgcaa aacgttggat tggtaccagg
     4861 agaaatatac aaacttatga tactgtgcgt tttttcagat attcatggta atcgtcttta
     4921 ttttgatttt ctggcggacg tttggaaagg aatggacatc gatcgtttct tttttttggg
     4981 agatgcgatc ggatattttc cggatgggaa tgaggtgttg gatcggctga aggagttcaa
     5041 tcccgtttgt gtaaaaggca atcatgaagc tatgctgttg ggattattac cattatcgga
     5101 tgaaaaagat gaggtttatc atctgaagga acaaaaagcg aatct
//
LOCUS       DAOSMM010000093         5128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_16386_length_5128_cov_20.369604, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000093 DAOSMM010000000
VERSION     DAOSMM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16386_length_5128_cov_20.369604"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK898_09500"
     CDS             <1..191
                     /locus_tag="PK898_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU20307.1"
                     /translation="IVNNSMEKYTLSAGLALLIGEHTTIYPHVMAGSFLAVWPMILLY
                     FLAQKQFVEGIAFSGIKG"
     gene            complement(277..363)
                     /locus_tag="PK898_09505"
     tRNA            complement(277..363)
                     /locus_tag="PK898_09505"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Ser,seq:cga)
     gene            450..1190
                     /gene="nth"
                     /locus_tag="PK898_09510"
     CDS             450..1190
                     /gene="nth"
                     /locus_tag="PK898_09510"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU20308.1"
                     /translation="MSYNNSDMDEFSSSTHENLPVHKAAVIYDRLIENFGVPNWPKMA
                     PLDEMINTILSQNTNDRNRDSAFVSLRHRFPTWEAVRDAPASEVIDCIRSAGLGNQKG
                     PRIQEILRQITEETGGLDLDFLKGWEPERIRSWLTGFKGVGMKTASIVMLFSLGIPAF
                     PVDTHIYRVTGRLGLRPAKMSVEQTHLQMAKLFPPENYGTAHINLILLGRRICFARNP
                     NCSECFLNDLCDYYSEKESIVQKNGTSR"
     gene            1187..1564
                     /locus_tag="PK898_09515"
     CDS             1187..1564
                     /locus_tag="PK898_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20309.1"
                     /translation="MKLALILILIGILAFGILRYLRPVILKKISLPDKWDKTLTVAIC
                     ILFPASLFFIFYFLFLGMISWLPASTAEIVWVSDFILMISFGIAGEWLFPTKKKANLS
                     LLCFFIGYALLCLWVIGNTVGTA"
     gene            1610..2812
                     /locus_tag="PK898_09520"
     CDS             1610..2812
                     /locus_tag="PK898_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOU20310.1"
                     /translation="MEPISIAKDIYWVGVNDRQSDLFEGLWPITPNGVSLNSYLILDE
                     KKVLIDLASDLLSEHYIQMLEQLIPIHQIDYIVLNHLEPDHTGALLKISSMAPNTIFL
                     GTAKAKEIVGNFYNLTENFRVVADGEELKIGRHTLKFVSIPFVHWPETMVTYDEEDQI
                     LFSCDAFGGYGALPGILFDDQCADLSFMEEEALRYYSNIVAAHSAHTKRAIAKLVNTP
                     IQMIAPSHGLIWRSHPERIFELYSKWANYSDGGREKTATVLYGSMYGNTRFFAESVIQ
                     AVAQKGLPVEVFDINSSHISYILPSLWKNQGVIICAPTYEGSIFPYMMNVLSMAKIKK
                     VMNRSAAYFGSYAWGSMAKKQFEDYCTEQKWDIVENRQFFGKPKSADFEMIPDFAERF
                     VKAVKEAV"
     gene            2824..3135
                     /locus_tag="PK898_09525"
     CDS             2824..3135
                     /locus_tag="PK898_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HOU20311.1"
                     /translation="MSATFIRRQEDFICEHCGKKVKGNGYTNHCPYCLYSKHVDINPG
                     DRASACGGLMEPIRAELRKNEVVLTHRCLKCGCLKVNKTAPEDNFDEILRIMQNSQNT
                     K"
     gene            complement(3163..4077)
                     /locus_tag="PK898_09530"
     CDS             complement(3163..4077)
                     /locus_tag="PK898_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20312.1"
                     /translation="MKLSEIPMADWQVIRDGEFLSLGLCGARPSVPILTFAGSEKYLK
                     TAVRNPDVSCILCPPELAASDLIRTSGKGICTCENIRVGFFKFHNELAKGDISDGYLR
                     GVFPVKIGRNAAISDDAILDSENIIIGDNAVIEPFVRIHANVQIGENSIIRSGTILGG
                     TGLEFIRTDDGGILPVTHCGGLVIGKNVEIQYNCNVSRSLFPWDDTVIGDDTKIESLV
                     HVAHGVKIGQRNLIAACACIGGSAELGNDVWVGPNATISSEVKVDDHSRISLGAVVVG
                     NVERGQTVTGNFAMNHEQFMQDQLRKML"
     gene            complement(4074..4796)
                     /locus_tag="PK898_09535"
     CDS             complement(4074..4796)
                     /locus_tag="PK898_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20313.1"
                     /translation="MTGDLSKTIYLTMDTDWAGDDVLSDSLTLIDELQVPVTIFITHE
                     TKLLETIRDHPLIRLGIHPNFYPLLQNHADQDYLSVIGEIKKIVPEAVSARSHGLIDS
                     TAILEAFKTQGITRDLNLFIPFSSGIELKPFRHFCGLLRIPYFYEDDAYCSENSGKTA
                     CEHILSSGDGLKVFNFHPIHLFLNTENMNRYLDAKPFQREYSQLIKYVNRDRQIGARA
                     FLKQVVECGRENRFTFDHVENL"
     gene            complement(4793..>5128)
                     /locus_tag="PK898_09540"
     CDS             complement(4793..>5128)
                     /locus_tag="PK898_09540"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOU20314.1"
                     /translation="YTGEMLVLSNGITQGEIGRFPEVTSQCEEIALKLGARSAINIQL
                     RFVDGKAYVFEINPRYSGTSSFRAMVGYNEPDLMIRKYLLNEEIEPHFAYKEGTILRG
                     LDEIFLEKR"
BASE COUNT         1392 a         1177 c         1137 g         1422 t
ORIGIN      
        1 tgatcgtcaa caattctatg gaaaaataca cgctgtcagc cggattggct ctgttgatcg
       61 gtgagcatac caccatctac cctcacgtaa tggccggaag tttcttggcg gtttggccga
      121 tgattcttct gtatttcctg gctcaaaagc aatttgtaga aggaatcgct ttttccggga
      181 taaagggata gaaggattct cgacaggatt tttatcgttt tctccttccg aaacagaaga
      241 gctgaaaatt ctatgataga attttcagct cttctgcgga gaaggtggga ttcgaaccca
      301 cggtagtcaa aagactacaa cggttttcga gaccgccccg ttcaaccact ccggcacctc
      361 tccatttctt gcttgagcaa ttatactaca tggaatcttc tgtttcttgc agtcctttga
      421 attctgtccc gtttcctgaa atttgagcct tgtcttataa taacagcgat atggatgaat
      481 tttcttcctc gacacatgag aacttgccgg tacataaagc cgccgtgatt tatgatcgtc
      541 tgattgagaa tttcggcgtt ccgaattggc cgaaaatggc gcctctggat gaaatgatca
      601 acacgatttt gtcgcaaaac acaaacgatc gcaatcgcga ttcggcattt gtgagtttac
      661 gtcaccgttt ccccacttgg gaggcagtca gagatgctcc ggcttcggaa gtaatcgact
      721 gtattcgttc agccggactg gggaatcaga aaggacctcg cattcaggag attctcagac
      781 agattacaga agaaaccggc ggcttggatt tggatttttt gaagggatgg gagcccgaac
      841 gcattcgttc ctggttaacc ggttttaaag gggtagggat gaaaacagcc tcgatcgtta
      901 tgcttttttc gctcggcatc ccggcttttc cggtcgatac acatatttac cgggtgaccg
      961 gcaggctggg attaagaccc gcaaaaatga gtgtcgaaca gacccattta cagatggcaa
     1021 aacttttccc gccggaaaat tacgggacgg cacacattaa tctgattctt ctcggacggc
     1081 gaatttgctt cgcccgtaat ccgaattgct ccgaatgctt tctgaatgat ctttgcgatt
     1141 attattcgga aaaagaatca atcgttcaaa agaacgggac ttcgcgatga aactcgcctt
     1201 aattctgatt ttgatcggca ttctcgcatt cgggatcttg cgatatttgc gcccggtgat
     1261 tcttaagaaa atatcccttc ccgataaatg ggataaaact ttaaccgtcg cgatctgtat
     1321 cctgttcccg gcatcgctct tcttcatttt ttattttctt ttcttgggaa tgatttcatg
     1381 gttgccggct tcgaccgcgg aaatcgtttg ggtgagcgat ttcattctta tgatcagttt
     1441 cggaattgcg ggagaatggc ttttcccgac aaaaaagaag gccaatcttt cattgctttg
     1501 tttttttatc ggttatgctt tgctttgttt atgggtcatc ggaaataccg tcggtacagc
     1561 ctgattatca aggcttagaa tagaattaat ttgattttgg ggaggaatta tggagccgat
     1621 ttcaatcgca aaagatattt attgggtggg agtcaacgac agacagtctg atctttttga
     1681 gggtttatgg ccgatcactc caaacggggt atctttaaac agttatctga ttttggatga
     1741 aaaaaaagtc ctgatcgatc tggccagcga tttattatcc gaacattata ttcagatgtt
     1801 ggaacaattg attccgattc atcaaattga ctatattgtc ctgaatcacc ttgaaccgga
     1861 tcacaccggt gccctcttaa aaatttcaag catggctccg aatacgatct tcttgggaac
     1921 ggcgaaagca aaggaaatcg tcggaaactt ttataacctt accgaaaact tcagggttgt
     1981 ggcagacggt gaagaattga aaatcggccg ccatacttta aagtttgttt cgattccgtt
     2041 cgtccattgg cccgagacca tggtcactta tgacgaagaa gatcaaatcc ttttttcctg
     2101 tgatgcattc ggtggttacg gcgctttacc gggaatcctt tttgatgatc aatgtgccga
     2161 tctttcattc atggaagaag aagcgctgcg atattattcg aatattgtcg cggctcacag
     2221 tgcccatacc aagcgggcga tagcaaaatt ggtaaatacc cccatacaga tgattgcgcc
     2281 ttctcacgga ttgatttggc gaagccatcc cgaaagaatc ttcgagctat actccaaatg
     2341 ggcgaactac tccgacggcg gacgcgaaaa aacagccacg gttctttatg gctccatgta
     2401 tgggaatacc cgattttttg ccgagtctgt tattcaagct gtcgcacaaa aaggactgcc
     2461 ggttgaggta ttcgatatca actcgtccca catcagttat atcctccctt ctctttggaa
     2521 aaatcaggga gtaattattt gcgcaccgac ctatgaagga tcgatttttc cttacatgat
     2581 gaatgttctg tcgatggcta agatcaagaa agtgatgaat cgaagtgcag cctatttcgg
     2641 cagctatgcc tgggggagta tggcgaaaaa acaatttgaa gattactgca ccgaacaaaa
     2701 atgggatatt gttgagaaca ggcagttctt cgggaaaccg aaatccgcgg atttcgaaat
     2761 gattccggat tttgccgaac ggtttgtaaa agccgtaaaa gaagcggtat aggttcgtga
     2821 ttcatgtctg ccacttttat ccgccggcag gaagatttta tttgtgaaca ttgcggaaag
     2881 aaggtcaagg gcaatgggta tacgaatcat tgcccttatt gtctttacag taaacatgta
     2941 gatatcaatc cgggggaccg cgcctcggct tgcggggggt tgatggaacc catccgggca
     3001 gaattgcgaa agaatgaagt ggttctcact catcgctgtc tgaagtgcgg ctgcctgaaa
     3061 gtcaacaaaa cagctccgga agataatttt gatgaaattt tgcgaattat gcaaaattca
     3121 cagaacacaa aatagattcc atatggataa gtataaaaag agctataaca tctttcgcag
     3181 ttgatcttgc atgaattgtt cgtgattcat cgcaaaattg ccggtcactg tttgccctcg
     3241 ctccacattg ccgaccacta ccgctccgag actgatgcgg gaatgatcat ccactttcac
     3301 ttcactggag atagtcgcat tcggaccaac ccaaacgtcg ttgcctaatt cggcggaacc
     3361 gccgatacac gcacaagcgg cgatcagatt ccgctgccct attttaaccc cgtgagcaac
     3421 atgcaccaga ctctcgatct tcgtatcatc accgataacc gtgtcatccc acggaaacag
     3481 agaacgggaa acgttacagt tatattggat ttcgacattt ttcccgatca ctaacccgcc
     3541 gcaatgagtc accggtaaaa tcccgccgtc atccgttcgg atgaactcca acccggtccc
     3601 gccgaggatg gtccccgatc gaataatact gttttcaccg atttgcacat tcgcatgaat
     3661 ccggacaaaa ggttcgatga cggcgttgtc cccgatgatg atattttccg aatccagtat
     3721 cgcatcatcg gaaatagcag cattcctgcc gatttttacg ggaaatactc ctcgcagata
     3781 accatccgaa atatctcctt tggctaattc attgtgaaac ttaaagaagc cgactcgaat
     3841 attttcacag gtacatattc cttttccgct tgtccgaatc aaatcggatg ccgccaactc
     3901 cggcgggcaa aggatacagc tgacatcggg attgcgaacc gcggttttca gatatttttc
     3961 gcttccggca aacgtcagaa tgggaacgga aggtcgggcc ccgcacaaac ccaaagaaag
     4021 gaattcgccg tcccgaatta cctgccaatc agccatcgga atttcgctga gtttcataag
     4081 ttctccacat gatcgaaagt aaaacggttc tcgcgcccgc attcaacaac ttgcttcaga
     4141 aaagctcgtg ctccgatctg ccggtcgcgg ttgacatatt ttatcagctg ggaatattcc
     4201 ctttgaaaag gtttggcatc caaatagcga ttcatattct cggtatttaa aaataaatgg
     4261 atgggatgaa aattaaatac ttttaaaccg tcccccgaag acaaaatatg ttcgcatgcc
     4321 gttttccctg aattttcaga gcaatatgca tcatcttcat agaaatacgg gatacgcagc
     4381 aatccgcaga aatgccgaaa aggtttcagc tcgattccgg aactgaaggg aataaacaaa
     4441 ttgagatcac gggttatccc ctgtgtttta aaagcctcca aaatcgccgt tgaatcgatc
     4501 aatccgtgag aacgtgcgct gaccgcttca ggtacaatct tcttgatttc gccgatgacg
     4561 gataaataat cctgatcagc gtgattctgc aacaaaggat aaaaattcgg atgaattccc
     4621 aagcgaatga gcggatggtc acgaatcgtt tccaataatt tcgtctcatg agtgataaaa
     4681 atcgttaccg gaacttgcaa ttcgtcaatc agagtcagtg aatccgaaag gacatcgtcg
     4741 ccggcccaat cggtatccat ggtcagatag atcgttttgg aaagatcacc tgtcaccgct
     4801 tctccaaaaa tatttcatcc aaacccctta aaatcgtccc ttctttataa gcgaaatgag
     4861 gctcgatttc ttcgtttaat aaatatttcc ggatcatcag atccggttca ttgtatccga
     4921 ccatcgcccg aaaagatgag gttccggaat atcgcggatt gatttcaaaa acatatgctt
     4981 ttccgtctac aaagcgtaac tgaatattta tcgccgaacg tgcccccagt ttcaacgcaa
     5041 tttcttcgca ctgagaagta acttcgggga atcgcccgat ttcaccctgt gtaatgccat
     5101 tactgagcac cagcatctct ccggtata
//
LOCUS       DAOSMM010000094         5112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_16464_length_5112_cov_18.346253, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000094 DAOSMM010000000
VERSION     DAOSMM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16464_length_5112_cov_18.346253"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PK898_09545"
     CDS             complement(<1..1307)
                     /locus_tag="PK898_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20315.1"
                     /translation="MRKRKFRNISVSTILLFGIFFLIGCKVQVYDLQPVIATGTPVLS
                     AAKESQTLVMRNTSGPLPAVTNTPPIHIAPTRIGESVPIEILELVMINGGSGWGIGKV
                     ANGQDKIIVKTTNGGANWKNVSSSQAVYENAGKNKEISAWFLDSNQAWVVYWDNENWS
                     STNQIPVWKTTDSGGNWEKSILPTEGYSLQYFKDVQIRFIDPQIGWVFAKMGKTEERE
                     YIGLFTTHDGGRSWNLMVSPDSVNLPSKGGKNGAVFRNATEGWISGSNAKDDPGSLLW
                     HSIDGGNTWNKQVLPQYLAEGIPSDLLSNRQYSCSLTPPKFVDYLYQYAWMKMVCQGG
                     ELAEPLGLLYWTYDGGNSWRVYQLPAAEGSLVFYGIYQGWYSKTAAPGSSYPYEILGT
                     SNGGENWNLIAQTAWKSNLQFITASIGWGVVEYEGHYAMVKTG"
     gene            complement(1319..2350)
                     /locus_tag="PK898_09550"
     CDS             complement(1319..2350)
                     /locus_tag="PK898_09550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU20316.1"
                     /translation="MKSTGTNLLESDRFFEEFQKAVAYRRKLVLSDPLRCCRLFNGFY
                     EGNPAFSLDLFAATLVIQSYSEGTVSPENWLAFIQKAVNPMIPGIKNVLLKEHFSDDI
                     EKRNGTILSGGPLDERIREWGVEYAIDLQLNRDSGFYLDNALLRKWLIDHCEGKRVLN
                     TFAYTGSLGMAALAGGATRIVQTDLNEKFLNQFHKTAEINGVSAERYKNIAGDFFKIM
                     ALMRKEEQLFDIVILDPPFYSKTRNGIVDLQKNFINLINKIRPLVADGGKIIAINNAL
                     YVSGSEMMDRLEEAFDDGYIQIDERIPVPESFYGGDLSRAKLPADPFPFNHPTKIVTL
                     NVKRKDGRR"
     gene            complement(2429..2647)
                     /locus_tag="PK898_09555"
     CDS             complement(2429..2647)
                     /locus_tag="PK898_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20317.1"
                     /translation="MAGEKDGKENNKAGKSGTGKESAECRKEEAQQNDKHLRDCDCCF
                     VDFLDDHNDDSILASEHYQSELINLIRF"
     gene            complement(2683..3036)
                     /locus_tag="PK898_09560"
     CDS             complement(2683..3036)
                     /locus_tag="PK898_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU20318.1"
                     /translation="MPIYSYQCDNCDYEFDIFQHFSDEALTECPKCHKNTLRKLYTPV
                     GIVFKGSGFYATDHRSPSGMMPSPHEHEAKESSGSESAETEGSKSVESSQSVEKPKAE
                     LHASKTKEKAEVKRG"
     gene            complement(3320..4981)
                     /locus_tag="PK898_09565"
     CDS             complement(3320..4981)
                     /locus_tag="PK898_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20319.1"
                     /translation="MKKLSIRYLLILIICSVTIFFNQNNVFAEQYDVDTGFRTEKDGF
                     GFENYGNVGCLDKACQQTFPIENLTAVEMRRLFGDRVCRKIQTDGSCELYKVAELWMN
                     KVNRAMSGGHCEGMAVLSTLFYAGVLDPNIFGSHSVNQLNLVGNTLLQREIAYWFATQ
                     WFMDDYMIEKDPVSQLKILISAYKENPRLLIPIAIYQRNYSKGHTVLAYAIEDKGNGI
                     YWLEVYDSNYPNQEKHMIIDVSNNSWTYTTLSFPDRSEIVYEGIGNSNPFQLAPINSR
                     LKQYVCDFCPVVSSAQITQPEPGFNRLTVDSGINVYIENPEGQKTGYDWKTGKTYAEI
                     PDLEINRTMRQTSAQLPVNLPYYLWLNAPDLHSWATFDVSFTSQGTVLYLQNVLESYE
                     YPNIIYKPGDSKQEENSNTETFEVVAFPDRLPTVQFVISNEQGECRFDLNILFKGMVD
                     PNLKVDIQIFHNPKAGRFGINIYPAKDAETEQYLQNTFTISGDFYLYTDQDEKHIAIN
                     NETPIEMRVNGNISIDYQAWQKNGTFFINGDLDGDSIVEIAKEFQ"
BASE COUNT         1397 a         1174 c          959 g         1582 t
ORIGIN      
        1 tcgccggttt tcaccatagc ataatgtcct tcatattcga cgacgcccca tccgatggaa
       61 gcggtaataa actgcaaatt gctcttccag gctgtctgcg caatgagatt ccaattttct
      121 cccccgtttg acgttcccaa aatttcatag ggatagcttg aaccgggtgc tgccgttttt
      181 gaataccacc cctggtaaat tccataaaaa accaatgatc cctcagcagc gggcaattga
      241 taaacccgcc aggaatttcc accgtcgtag gtccaataaa gcagcccaag aggctcggcc
      301 agctcacctc cctgacagac cattttcatc caggcatact gataaagata atccacaaat
      361 ttcggcggcg tcaagctgca tgaatattga cgattgctta acagatccga tggaatgcct
      421 tcggcaagat attgtggtaa aacctgctta ttccaagtgt taccgccgtc aatgctatgc
      481 cataataaac tgccgggatc atctttggca ttgctgccac tgatccatcc ttctgtcgca
      541 ttgcggaata cagctccgtt ttttcctcct ttggaaggta agtttacgga atccggcgat
      601 accatcaggt tccaacttct cccgccatca tgggtagtga ataatccgat atattcccgt
      661 tcttcggttt tccccatttt ggcgaaaacc caacctattt gtggatcgat aaaacggatt
      721 tgtacatcct taaaatattg cagggaatat ccttctgtcg gcagaatcga tttttcccaa
      781 tttccaccgc tatcggtcgt tttccatacg ggaatctgat tggtagaaga ccaattttca
      841 ttgtcccaat aaaccaccca ggcttgattt gaatccaaga accaggctga tatctcttta
      901 tttttaccgg cattctcata aaccgcttga gaagaagata cattcttcca atttgcgccg
      961 ccgttggttg ttttcactat gatcttatct tggccgttcg caaccttgcc gattccccac
     1021 ccgctccctc cgttgatcat cactaattct aatatctcga tcggaacact ttcaccgatt
     1081 cgcgtcggag cgatatgaat cggcggggtg ttggttacgg cgggaagagg tccggaagta
     1141 tttcgcatga caagtgtctg actttctttc gctgcggaaa gtaccggtgt accggtcgcg
     1201 ataaccggtt gaaggtcata aacttgaact ttgcaaccga tcagaaagaa gatgccgaaa
     1261 agcagaatcg tagaaacaga aatattccga aattttcttt tccgcataag cactcctttt
     1321 atcttctgcc atccttccgt ttgacattca aggtaactat tttcgtcggg tgattgaaag
     1381 gaaaaggatc cgcaggcaat ttcgcccggg ataaatctcc gccataaaaa gattccggta
     1441 ccgggattct ctcatcgatt tggatatatc cgtcatcaaa agcttcttcc agccggtcca
     1501 tcatctccga gcccgaaaca taaagtgcat tgttgatagc gatgatctta cctccatccg
     1561 ccaccaaagg gcgaatcttg ttaataaggt tgataaaatt tttttgtaaa tcaacaatgc
     1621 cgtttcgtgt ttttgaatag aatggcggat ccaaaattac gatatcaaaa agttgctctt
     1681 cttttcgcat aagagccata attttgaaaa aatcaccggc tatgttttta tatctttcgg
     1741 cggaaactcc gttgatctcg gctgtcttat gaaattgatt aagaaatttc tcattcagat
     1801 ccgtttgaac gatgcgagtt gctccgccgg ctaaagctgc catcccaagg cttccggtat
     1861 aagcaaatgt attcaatacc cgtttccctt cgcaatgatc gattaaccat ttccgcaata
     1921 atgcgttatc caaataaaac ccgctgtctc tgttcaattg taaatcgatc gcatattcca
     1981 caccccattc ccgaattcgc tcatccaaag gtccgccgga taaaatcgtg ccgtttcttt
     2041 tttcgatatc gtcagaaaaa tgttctttca gtaggacgtt ttttattccg ggaatcatcg
     2101 gattcaccgc tttttggatg aaagccagcc aattttcggg cgaaacagtt ccttcggaat
     2161 aagattgaat taccagtgta gctgcaaata aatcaaggct gaaagccgga tttccctcat
     2221 agaatccatt aaacaggcga cagcaccgca ggggatcgga taaaaccaat ttccggcgat
     2281 aagccactgc tttttgaaac tcctcaaaaa agcgatctga ttccaacaaa ttcgttcccg
     2341 ttgatttcat cctatcatct ctctcaaaaa atctttatat tctatagatt tttttgttat
     2401 ctcaaaaatt taaccacaaa aacctcattc aaaatcgaat gaggtttatc aattcggatt
     2461 gataatgctc agaagctaaa atcgaatcgt cgttatgatc atcgagaaaa tcaacaaaac
     2521 agcaatcaca atccctaaga tgcttatcgt tctgttgcgc ttcttctttg cggcattctg
     2581 ccgattcttt tcctgtaccg ctctttcctg ctttgttatt ttctttgcca tctttttcac
     2641 ctgccacccg gaagcattta ttccgatctc agcttcctcg gattatcctc ttttaacttc
     2701 cgctttttct ttcgttttgc tcgcgtgtaa ttccgctttc ggtttttcaa ccgattgaga
     2761 gctttcaacg gatttcgaac cttccgtttc ggcggattcg gatccggagc tttctttcgc
     2821 ttcatgttcg tgcggtgaag gcatcattcc ggatggtgaa cggtgatcgg tagcgtaaaa
     2881 ccccgatccc ttaaacacga tccctaccgg tgtataaagc ttccgcaagg tatttttatg
     2941 acatttcgga cattcggtta aagcttcatc cgaaaaatgt tgaaagatat caaactcata
     3001 atcacaatta tcacattgat aagaataaat tggcattttc aaacctcgat cattcaatcg
     3061 tattgaaatt aatttatcct tttatctttt ttttgtattt ttgaatcagc atagacgata
     3121 aaaaaatcca atacgcgttc tattatatca aattatcaat tttcgtttaa ttttacgaca
     3181 tgttttatcg tccggacgat ctctgatatt tctctaatac atattccttc attttcgatg
     3241 ttttatcagt ggtttcgctc agaccgagaa tgagatatgc aaacattccg gataatattc
     3301 tgtctgaaac atactcgatt tattgaaact ctttggcgat ttcaacgatt gaatctccgt
     3361 ccaaatcacc atttatgaaa aacgtcccgt ttttttgcca agcttgataa tcgatggaaa
     3421 tgttcccgtt tacccgcatt tcgatcggcg tttcattatt gatggcaata tgtttttcgt
     3481 cttgatctgt gtaaagataa aaatcgccgc tgatcgtaaa agtattttgc agatattgtt
     3541 ccgtttcggc atcttttgcc ggataaatat tgatcccaaa tcgacctgct ttgggattat
     3601 gaaaaatttg aatatcaact ttcaaattgg ggtcgaccat ccctttaaat aaaatattca
     3661 aatcaaaacg acattctccc tgttcattac tgatcacgaa ttgaacagtc ggtaaacggt
     3721 ccggaaaagc caccacctcg aaagtctcgg tattggaatt ctcctcttgt ttagaatctc
     3781 ccggcttgta aatgatattc ggatactcat aagattccaa tacattctgt aaatagagaa
     3841 cggttccttg tgaagtaaag ctgacatcaa acgttgccca actatgcaga tcaggggcgt
     3901 tcaaccacag atagtaagga agatttaccg gtaattgggc tgaagtctgc cgcattgtcc
     3961 ggttgatctc caaatcggga atctccgcat acgtttttcc ggtcttccag tcgtatcccg
     4021 ttttttgccc ttccggattt tcaatataca cattaattcc agaatcaact gttaaacgat
     4081 tgaatcccgg ctcgggctgg gtgatttgtg cggatgaaac gaccggacaa aaatcacaga
     4141 catattgttt gagtcgtgaa ttgatgggag ccaattgaaa gggattggaa ttaccgatcc
     4201 cttcataaac aatttccgat cgatccggga aagaaagggt tgtataggtc catgaattat
     4261 tcgagacgtc gataatcata tgcttttcct gattcgggta attactgtca tatacttcca
     4321 gccaataaat tccattccct ttatcttcga tcgcataagc gagcacagtg tgacccttgc
     4381 tgtagtttct ttgatagatt gcgatcggaa tcaataatcg cggattttct ttataggcag
     4441 aaatcaagat ttttaattgt gagaccgggt ccttttcaat catgtaatca tccataaacc
     4501 attgggttgc aaaccaatag gcgatttccc tttgtaacaa tgtattcccg actaaattta
     4561 attggttaac cgaatggctg ccaaaaatat tgggatccaa gacgccggca tagaacaatg
     4621 tactcaggac agccattcct tcacaatgtc cgccggacat tgcccgattg actttattca
     4681 tccagagttc cgcaacttta taaagttcac agctgccgtc ggtttgaatt ttccgacaaa
     4741 cgcgatcgcc aaataatcga cgcatttcaa cggctgtcaa attttcaatc gggaatgtct
     4801 gctgacatgc cttatccaag caaccgacgt ttccgtaatt ctcaaaaccg aacccatctt
     4861 tttcggtccg aaatccggta tccacatcat attgttccgc aaaaacgtta ttctgattga
     4921 aaaaaatagt aacggaacaa attatcaaaa taaggaggta ccggatactt aattttttca
     4981 tttttcattg ctctttatca ttataaaggg attctttgaa accgagagat ggagggtgtt
     5041 cgaaaaacgg cgtggatttt tgggatttgt atcggaaatg tccaaaagct ctggatttgt
     5101 cggttcgatt ct
//
LOCUS       DAOSMM010000095         5027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_16817_length_5027_cov_11.219831, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000095 DAOSMM010000000
VERSION     DAOSMM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16817_length_5027_cov_11.219831"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK898_09570"
     CDS             <1..419
                     /locus_tag="PK898_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOU20320.1"
                     /translation="LTVQKEVAQRICAQPGDLSLLALSVQIFGSVSIPLTIPADAFFP
                     QPKVDSSVVKIDVYTEPLVSVAELDDFFRLAKAGFGQKRKTLRNSLSSDLRLSTSEVE
                     AALAKAGIDPMRRAETVSIEEWKRLVESIPKNKNSR"
     gene            complement(504..713)
                     /locus_tag="PK898_09575"
     CDS             complement(504..713)
                     /locus_tag="PK898_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOU20321.1"
                     /translation="MSDKVKGTVKWFNESKGYGFLSQPEGEDIFVHYTAIQGNGFRTL
                     KEGAEVEFNIEHGPKGLQASNVIVL"
     gene            complement(1019..1756)
                     /locus_tag="PK898_09580"
     CDS             complement(1019..1756)
                     /locus_tag="PK898_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20322.1"
                     /translation="MNGIKNLIKEENIFGRSDTEKETCSNGINFTDQYRQTVLPVFYY
                     LFTFNPILYDANGNRLPDPDQETQRELWKYEDSLRNHLSEGDNFKYIGTMHTFTIPKS
                     GIIFPVYAKQNVYERSFPEKEAFAQIEFNLNRIQPANLPFHVNQHIQIGSIDFILETV
                     EKKPNGGYSFLFDGTDNKVIQCQIDPIGYETNLKGSSSFNPDNPFHFRQELFFQGNPS
                     EKISIRISQPAVLGDLISFIGSWSPQE"
     gene            1956..2543
                     /gene="cyaB"
                     /locus_tag="PK898_09585"
     CDS             1956..2543
                     /gene="cyaB"
                     /locus_tag="PK898_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOU20323.1"
                     /translation="MKDSEKNQEIEAKFPVKDLRKIQELLEFHQAELLMDKSYEHNIL
                     LDTPTNELLEENARLRLRKEGSESILTYKRTRKNENNIAYREEIETRVDHFENTRLIL
                     NRLGFLTFFEYEKYRSTYRLGATTIMLDETPIGFYLEIEGPDEETIHRTASLLEIDWN
                     QRTDKSYLQVFQEWAAENGYTGRDMLFSSAPFLRG"
     gene            complement(2532..3845)
                     /locus_tag="PK898_09590"
     CDS             complement(2532..3845)
                     /locus_tag="PK898_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU20324.1"
                     /translation="MKLAIPLILQYLLSSSVTLVDNIMVGALGDVSLAAVNICTQFYL
                     KLFHIVIFGLVAGASILTTQYWGVHDSKNIHRIMGLEMIVGSGLGVLFSILTAFFPEK
                     ILGFYSRDPQVIQAGSLYLSTFSLIFLLHPIALIFASAHRAIGDTKLPMIAIALSLAI
                     KLILNYFLIYGNWGFPKMGIAGAAYATLIAKISETALLLFFTYKSKSPVAATFNTLFD
                     FDKKLIIKTTKNVLPVLINETLWGLGTNVYSAIYAKISTTAIAAVSAVSPIDTLMFTL
                     IYSFGDACGILVGNFLGRNEQDKAYEYGIFTTFFMMLVGCLTGIVVFCLRDSILNLYN
                     LSAESYASARIILTILTASLCFRAITYTIFIGVLRAGGDVRFCLAVDAGSIWLIGIPV
                     SVVLAFGFHLPIETVYLGFMCDELFKVIVVTHRLRTRKWMNVLTA"
     gene            complement(4036..>5027)
                     /locus_tag="PK898_09595"
     CDS             complement(4036..>5027)
                     /locus_tag="PK898_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20325.1"
                     /translation="LITGPDSFEIQMSAKGTVKGNHISLFSFDGTPLTEIDLSVNPTD
                     PNQLIIYGMDMADQSSKTYYLEISNREISLDSDTDSIFSLWFRPGERILIEIPSSESE
                     VELAFDKKAYKITGTVSSPEQTMEIELELNPLRNRIALALNGTPVVWIESDPAKNSLD
                     LVFADTELLMEFDDDAHEILGTLYAADQQSQFKLICDKPSNSLALDMVIEGEMTELFK
                     LAFSKQANGVSLNSAGGLIFSATIDEEKGTMTINDSSGFRVLDESQILDLINSSIKTG
                     PEAETEVIDNFLNEEEETAVPEEEIIPTAAPISSPTEIPTTVPVAVPTLDAGI"
BASE COUNT         1464 a         1045 c         1084 g         1434 t
ORIGIN      
        1 tcctgacggt tcagaaagag gttgcgcagc gtatttgcgc gcaacccggg gatcttagct
       61 tgttagcatt aagtgtgcaa atttttggtt ccgtatctat cccgttgacg attccagcgg
      121 atgccttttt cccgcagccg aaagtggatt cgtctgtggt gaaaatcgat gtatataccg
      181 aaccgctagt ttcggttgcc gaattggatg atttttttag gttggcgaaa gcggggtttg
      241 gtcaaaaacg gaaaacgctg cgaaattcat tatcttccga tctccggctt tcgacttcgg
      301 aggtcgaagc tgcattggct aaagctggaa tcgatccgat gcgtcgtgct gaaacggtgt
      361 cgatagagga atggaagcgt ctggtcgaat cgattccgaa aaataaaaac agccgataaa
      421 atcggctgtt tatttctaat accttggtac agatttgaaa ttacaaaaaa cttcatttcc
      481 cgaaggttta attctctgtt cgatcagaga acaattacat tgctggcctg cagacctttc
      541 ggaccgtgtt caatattgaa ttcaacttct gcgccttctt tcagagtgcg gaatccgttg
      601 ccctgaattg cggtgtagtg aacgaaaata tcttcacctt ccggctgtga aagaaaacca
      661 taaccctttg attcattgaa ccacttgact gtacctttga ccttgtcact cattctgaaa
      721 atctcctaaa aatttccctc ggctctccga ggtttgaaaa tttggaacgc ttttggctat
      781 tccattgact aaatttggat aagaagaagc tgatagaaaa ccgctttccc atgtaaattg
      841 aggaaagcgg aacttcattg cctaatctcc ttaatcactg ctgtatttat accactattt
      901 ttctttaatg gatgcgcata ttacaaacat catcaagatt cattatcaat gtaacagcat
      961 gctttctata ttttcacatt attacctttc gtggcatcat cgtctcccgc ttcatggatc
     1021 attcttgagg agaccatgag ccgataaagg agatcagatc acccaaaaca gcgggttggc
     1081 tgatgcggat cgatattttt tcgctcggat tgccttggaa aaataattcc tgacgaaaat
     1141 ggaaaggatt atccggatta aagctgctgc tgcccttcaa gttcgtttcg taaccgattg
     1201 gatcgatttg gcattgaatg accttgttat cggtgccgtc aaagagaaat gaataaccac
     1261 cattcggctt tttttcaacg gtttctaata tgaagtcaat ggaaccgatt tggatatgct
     1321 ggttgacatg aaacggtaga ttggcaggtt gaattctatt aaggttaaac tcaatttgag
     1381 cgaatgcttc tttttcggga aaggagcgtt cataaacgtt ttgtttcgca taaacgggga
     1441 aaataatacc cgacttcgga atcgtaaaag tgtgcattgt accgatatat ttgaaattat
     1501 ctccctcgga aagatgattg cgtaagctat cttcatattt ccaaagttct cgttgagtct
     1561 cctgatccgg atccggcagc cggttaccat ttgcatcata aagaatcgga ttaaaagtga
     1621 ataagtaata aaaaaccggt aatacggttt gacggtattg atctgtaaaa ttaatcccat
     1681 ttgaacaggt ttccttttcc gtatcactcc tgccaaaaat attctcttct tttatcaggt
     1741 ttttaattcc attcattggt tttcctttat cggatccttc aatttaattg tagctacaat
     1801 aataaagtcg ttttgaggga aggaaatgtg acaaaaatgg aaggggaaaa tggcaaaaga
     1861 ccattttaga gcgattaaat tgattctgta cttttttact tggaaagatt taacctgaaa
     1921 aaggggtaaa atttgtggtt atggaaggcg gacttatgaa agattccgaa aaaaatcagg
     1981 aaattgaagc aaaattcccg gtgaaggatt tgcgaaaaat tcaagaactg ttggaatttc
     2041 atcaagctga attgttgatg gacaaatcct atgaacataa cattttattg gatactccga
     2101 ccaatgaatt gctggaggaa aacgcccggt tacgcttgcg aaaagagggt tcggaatcta
     2161 tattgaccta taaacgtact cgaaaaaatg aaaataatat tgcttatcgg gaagaaatcg
     2221 aaacacgggt tgatcatttt gaaaacaccc gcctgatatt aaaccgtttg ggttttctga
     2281 ccttttttga atatgaaaaa tatcgcagta cttacaggtt gggagcaact acgatcatgc
     2341 tggatgaaac gccgatcggt ttttatctgg aaatagaagg acccgatgaa gagaccattc
     2401 atcgtacggc ttctctgtta gagatcgatt ggaatcagcg cacggataaa agttacctgc
     2461 aggtatttca ggaatgggct gccgaaaacg gctataccgg tcgcgatatg cttttcagct
     2521 cggctccctt tttacgcggt taagacattc atccattttc gggttcgcaa acgatgagtg
     2581 acgacgatca ctttgaacag ttcatcgcac ataaaaccca aatagaccgt ctcaatcggc
     2641 aggtgaaaac cgaaggctaa aacaacgctg accggtattc cgatgagcca aatagaaccg
     2701 gcgtccaccg ccaagcaaaa tctgacatct ccccccgccc gtagaactcc gataaaaatc
     2761 gtataagtga ttgcccgaaa acacagagag gctgtcaaaa ttgtcaaaat tatccttgcg
     2821 cttgcataag attccgcgga gagattgtaa agattcagaa tcgaatccct taggcaaaat
     2881 acgacgattc cggttaagca tccgacgagc atcataaaaa aagtcgtgaa aattccatat
     2941 tcgtaggctt tatcctgctc atttctcccc aagaaattcc cgactaaaat gccgcaggca
     3001 tccccgaaag aatagatcag cgtgaacatt aaagtgtcta tggggctcac agcggacact
     3061 gcggcgatgg cggtggttga aattttggca tagatcgctg aataaacgtt cgtccccagc
     3121 ccccaaagag tttcgttaat caatacgggg agcacattct tggttgtttt tatgattagt
     3181 tttttgtcaa aatcaaataa tgtattgaag gttgcagcga ccggggactt tgatttgtaa
     3241 gtgaagaaaa gcaataatgc ggtttccgaa attttcgcga ttaaagtcgc ataagccgct
     3301 ccggcaatgc ccattttggg gaacccccaa tttccgtaaa tgagaaagta atttaatata
     3361 agcttaatcg ccaatgataa agcgatagcg atcatcggta attttgtatc accgatagcc
     3421 cgatgagcac tcgcgaagat taatgcaatc ggatggagta aaaagatcag cgaaaaggtg
     3481 gacaggtata acgatccggc ttgaatgact tgagggtctc gggaatagaa tcccagaatc
     3541 ttctccggga aaaaggcggt taagatcgaa aataaaacac cgagtccgga accaacgatc
     3601 atttcaagcc ccataatgcg atggatgttc tttgagtcgt gaacgcccca atattgagtc
     3661 gtcaggatcg aagcaccggc gaccaaaccg aaaatcacga tatggaataa ctttagataa
     3721 aattgggtgc aaatgttaac agccgccaga gaaacgtctc ccaatgctcc aaccatgatg
     3781 ttatccacca atgttacaga agaagataac aggtattgga ggatcaacgg tattgcaagt
     3841 ttcatgatct gccgaaaata gattctgtct ggttttttga tttttatcat gacctcactg
     3901 actgcacgga ttgaaacctc cgagcatttt gcaaaaagaa actgagctga taaatatgac
     3961 ctttcgccgg cgcagctcag ttccttataa aattgtaaga tatttcaaac tattttaatt
     4021 gatctttccc ccgatttata ttcccgcatc caatgtgggg accgctacgg gaaccgttgt
     4081 cggtatttcc gtaggggatg agatcggagc tgccgtcgga attatttctt cctccggaac
     4141 agcggtctct tcctcttcat ttaagaaatt atcgatgact tccgtttccg cctccggtcc
     4201 tgtttttatg gaagaattga ttaaatcaag gatctgagat tcatccaata ctctgaagcc
     4261 cgaagaatca ttgatcgtca tggtaccttt ttcttcgtcg attgtagcgg aaaaaatcaa
     4321 tcctccggcc gaattcagtg agacaccgtt tgcctgttta ctgaaggcaa gcttgaagag
     4381 ttccgtcatc tctccttcaa tcaccatatc caacgccagc gaattactcg gtttatcaca
     4441 gatcagtttg aactgcgatt gctggtcagc cgcatataac gtgccgagaa tttcatgtgc
     4501 atcgtcatca aattccatca agagctcagt atcggcgaat acaagatcca aactgttttt
     4561 ggccggatcg gattcgatcc aaacgaccgg agtgccgttg agcgctaaag cgatccgatt
     4621 tctcagcggg ttcaattcca attcaatctc catggtttgt tccggtgagc tgaccgtgcc
     4681 ggtgattttg tatgcttttt tatcaaaagc cagttccact tcgctttcgg aggaaggaat
     4741 ctcaataaga attctttctc ctggcctaaa ccacaatgaa aaaatagaat ccgtatccga
     4801 atccaaggat atctcccgat tactgatttc cagataatag gttttactgc tttggtccgc
     4861 catatccatt ccatagatta tcaattgatt gggatcggtc ggattaacgg acaggtcaat
     4921 ctcggtcaag ggtgttccgt cgaaactgaa aagagaaatg tggtttcctt taaccgtgcc
     4981 tttggctgac atttggattt cgaaactatc gggtccggtt atcaaaa
//
LOCUS       DAOSMM010000096         5016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_16868_length_5016_cov_10.352550, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000096 DAOSMM010000000
VERSION     DAOSMM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16868_length_5016_cov_10.352550"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..976
                     /locus_tag="PK898_09600"
     CDS             146..976
                     /locus_tag="PK898_09600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU20326.1"
                     /translation="MDIKAILLDFDGTALQRDQVFISCRNMYAIRQAIAKGIEIIPCT
                     GRCEDMFPPQIEAEKRIRYWITASGTRIVDRNEKKVLYQCVFSPEESAQLCEIFENQD
                     IYTEISAEGHIYLEKKINDHLERYPVPSHHVWFVESGRQIPVEQPSKYFLEHQIGIEK
                     CNLYGIPDEKQQKISSALNSTGLIAPPANAEKDIQFFPRRLDKIQAIEFLLKKLGLEF
                     ENVMSFGDSSMDADIIEKAGFGIAMGNSPDWIKAKAKYITKPYFEDGVAEAIEKFILY
                     "
     gene            1037..2080
                     /locus_tag="PK898_09605"
     CDS             1037..2080
                     /locus_tag="PK898_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU20327.1"
                     /translation="MKKIFTVLICVILMSVFVVSPLNVSAVSTDLQPTQSLVLYTSAS
                     TTEYELIVGLFKEKYPDVEVEIVSAGTGELASKIVAEAANPQGDVLMGGGTSSYSAIA
                     DYLDEYVSPNLDSIFEEFKPPVSKWTPVYINVNSIIVNNKLINELGVTVDGWESLTNE
                     KLKGNIAFADPSASASALEQLVNMLTAMSTTDSPDGGWDFVKKFLINLDGKISSSSSA
                     CYKSVVEGEYAVGLTNEDKAISYIAAGADVSVVYPKEGITLRTSNIGVIKNGPNTYNA
                     QLFIDFVTSQECQKEMESKLFVRPARKDVPMTTKGRITTDQIKSLPYPTEWVEANSEN
                     VKVKFQDLLTSIQ"
     gene            2148..3251
                     /locus_tag="PK898_09610"
     CDS             2148..3251
                     /locus_tag="PK898_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20328.1"
                     /translation="MAVSIHIKNVMKKYDDNVVIPNLSLDIAKGELFTLLGPSGCGKT
                     TLLRMIAGFNTIEKGTIAFDEKIINDIPVHQRNFGMVFQSYAIFPNMTVYGNIEYGLK
                     NKHLSKEEIKKRVFEIMDTVQITPYKDRYPDKLSGGQQQRVALARAIAVRPQVLLMDE
                     PLSNLDAKLRLEMRNVIRDIQKRVGITTVYVTHDQEEALAISDRIAIMNNGEIYQVEV
                     PEKIYLRPYNTFVANFIGHSHCFQATVTESGEMTVIKLETGLTVILKNVKPLAVGEVV
                     LVYVRPEEFVLCDESQGMPGIIKSRQFLGKFINYFVDFGTKNLTEVSVNTNSANMIFS
                     PGEKIWMRVDSERVNLFAKDSETTLMEGVQSHV"
     gene            3244..4902
                     /locus_tag="PK898_09615"
     CDS             3244..4902
                     /locus_tag="PK898_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU20329.1"
                     /translation="MFNRKRIRFDFWNFVTLFSLVFFGMFLVIPVGKMILYAFQGKGV
                     EGFTLANFIKFFSRRYYVSALTNSIKVVLSATFLIILFGVPLAYITTTYKIRWKRFID
                     VLIIISMLSPPFIGAYSWILMLGRNGTITVFFKNVLGISLPDIYGFRGILLVFVLKLF
                     PYIYMYTKGALKKVDASLGEAAESLGYHGIKKILTVDLKLITPTILAGATVVFLRAFS
                     DYGTPRLIGEGYITLPVIIYQEWVSETGSNAYFASSVAMIMMTVAAIVFLIQQQISNK
                     KNYTMSSLNPPQPKQLHGIKNFFAHFFVYFIVFLATLPQIYIITISFRNNNGMMWTEG
                     YGFGNYQAVFQKATNSILNTFKFGIIAIFIVIIFGTLFAYLTVRRKNIFSKILDVFVM
                     FPYVIPGTIFGLILLMTYNSGPIILSGTAAIIIISYVIRRMAYTVRSSSAILRQINPN
                     IEEASISLGYSSLPTFFNVTMPAMVPGIISGAILSWITIINELSSTLMLYTSKTATMS
                     VAIFQEINRGGYGTAAALSTILTFTMVVSLLLFFRFTKSEEIDL"
BASE COUNT         1506 a          943 c         1052 g         1515 t
ORIGIN      
        1 ctcgatcaaa gacttgtttt acaatttgta taggattttg aaaatcgcag tttgttttat
       61 gtcagatatt cttattaaat aacgattaaa tctatttatc gttaaaaaca tttttaaatt
      121 gattatcttg cctaaaggag tatttttgga tatcaaagcc atattacttg atttcgacgg
      181 tacagcattg caaagggatc aagtatttat atcttgtcga aacatgtatg cgatcaggca
      241 agcgattgca aaagggattg aaattatccc ctgtacaggc agatgtgaag atatgtttcc
      301 ccctcagatc gaagccgaaa aaagaatcag gtattggatc acagcctcag gcacgcgtat
      361 cgttgacaga aacgaaaaga aagtattgta tcagtgcgtt ttttcacctg aagaatctgc
      421 ccaattatgc gagatatttg aaaatcaaga catttatacc gaaatttcgg cggaaggtca
      481 tatttatctt gagaaaaaaa taaatgatca tttggaacgt tatcccgttc cttctcatca
      541 tgtatggttc gtcgaatcgg gaagacagat ccccgttgag cagccctcaa aatattttct
      601 tgagcatcaa atcggcatag aaaaatgcaa tctttacggc ataccggatg aaaagcagca
      661 gaaaatcagc tccgcgctta attccacagg tttgattgct cctcccgcaa atgcggaaaa
      721 ggacattcaa ttttttccgc gaagactgga taaaatccaa gcgatcgaat ttcttttgaa
      781 aaaattaggg cttgaatttg agaatgttat gagcttcgga gacagttcta tggatgcgga
      841 catcattgaa aaagccggct ttggaattgc gatgggaaat tcgccggatt ggattaaagc
      901 gaaagcaaaa tatataacaa aaccatactt tgaagacgga gttgctgaag ctatcgaaaa
      961 atttattctg tattaatggt taatcccctc aaaatttcag agggatcgca agagtattat
     1021 caataaggag gattttatga aaaagatttt tactgttcta atttgcgtta ttctaatgtc
     1081 tgtttttgtt gtttcgccgt tgaacgtatc tgccgtatca acagatcttc aaccgactca
     1141 atcattggtt ttatatacgt ctgcgtcgac gacagaatat gaattgatcg tcgggttgtt
     1201 caaagaaaaa taccccgatg tcgaagttga aattgtcagc gcgggcacag gtgagcttgc
     1261 aagcaaaatt gttgccgagg cggcgaatcc gcagggcgat gttttgatgg gaggcggcac
     1321 ttcaagctac tcggcgatcg ccgattacct cgatgaatat gtcagtccca atctcgattc
     1381 gattttcgaa gaatttaaac cgcccgtatc aaaatggact ccggtttata tcaatgtaaa
     1441 cagcatcatt gtcaataaca aattaattaa tgagctgggt gtgaccgtag acggttggga
     1501 atctctaact aatgaaaaac tcaagggaaa tatcgcattt gccgatcctt ctgcatccgc
     1561 atccgctttg gaacaattgg taaacatgct gactgcgatg agcactaccg attcgcctga
     1621 cggaggttgg gactttgtaa aaaagttctt aataaatctt gacggcaaga tttcatccag
     1681 ttcttccgca tgttataaaa gcgttgtcga aggggaatat gcggtcggtt tgacaaatga
     1741 ggataaagcg atttcttata tagccgccgg ggctgatgtc agtgtggttt atccgaaaga
     1801 agggatcact ctgagaacca gtaatatcgg agtgattaaa aacggcccca atacctataa
     1861 tgcacagctc ttcattgatt tcgtgacttc gcaagaatgc cagaaggaaa tggagtcaaa
     1921 attgtttgtt cgcccggcaa gaaaagatgt tcctatgacc acaaaagggc gaatcacaac
     1981 cgatcaaatt aaatctctac cctatccgac agagtgggtt gaagcaaact cggaaaatgt
     2041 caaagttaaa ttccaagatc ttttgacatc cattcaataa ttattatgta tagaagatgc
     2101 ccgtttgcaa atatttgcga cgggcatctt tcaagaggaa gaatcagatg gcagtatcaa
     2161 tccatatcaa aaatgtaatg aaaaaatatg atgacaatgt tgtgatcccc aacttatctt
     2221 tagatatcgc caagggtgaa ttgtttacgc tcttgggacc ttcaggctgc gggaaaacaa
     2281 ctcttttgcg aatgattgcg ggatttaata ccatcgaaaa ggggaccatc gcttttgatg
     2341 aaaaaatcat taatgatatt cctgttcatc aacgaaattt tggtatggta tttcagagtt
     2401 atgcaatatt tccgaatatg acggtttatg gaaatatcga atatggattg aaaaataagc
     2461 atctttccaa ggaagaaata aaaaagagag tatttgagat catggacacc gtccagatta
     2521 cgccctacaa agatcgctat cccgataaac tttccggcgg gcagcagcaa agagtggcgc
     2581 ttgcgcgggc tatcgcggtc cggcctcagg ttcttttgat ggatgaaccg ctttccaatc
     2641 ttgatgctaa actgcggctt gagatgcgga atgtaattcg ggatattcaa aaacgggtgg
     2701 gaattactac cgtatatgta actcacgatc aggaagaagc acttgcaatt tcagaccgaa
     2761 tcgccattat gaataacgga gaaatttatc aggttgaagt tcccgagaaa atttatcttc
     2821 gtccctataa tacctttgtt gccaatttta tcggtcactc tcattgtttc caagcgaccg
     2881 taacagaatc gggtgaaatg actgtgatca aactcgaaac gggacttacc gttattttaa
     2941 agaatgtaaa acctcttgcc gtcggcgagg tagttttggt atatgttcga cccgaagaat
     3001 ttgtattatg tgatgaatcg caagggatgc cggggatcat taaaagcaga caattcctcg
     3061 gaaaatttat taattatttt gtcgatttcg gaacaaaaaa tttaactgaa gtttccgtaa
     3121 acacaaacag tgcaaatatg attttttctc ccggagaaaa aatttggatg cgagtggatt
     3181 cagagcgggt caacctattc gcaaaggatt ccgaaacaac attaatggaa ggggtgcaga
     3241 gtcatgttta accggaaacg aatccgattt gatttttgga actttgtaac cctcttctca
     3301 cttgtttttt ttggaatgtt ccttgttatt ccggtaggaa aaatgatcct ctatgcgttt
     3361 caaggaaaag gcgtagaggg attcacactt gcaaatttta ttaaattttt ttcacgccgt
     3421 tattatgtaa gtgctttgac aaacagtata aaagttgttt taagcgcaac ctttttaatc
     3481 attctttttg gtgtaccgct tgcctacatt acaaccactt ataagatcag atggaagaga
     3541 tttattgatg tcttgatcat catctcgatg ttatcccccc cattcatcgg tgcttattct
     3601 tggatattaa tgttaggtcg taacggaacg attacggttt ttttcaaaaa cgtattgggg
     3661 atctcactgc cggatatcta cgggtttcgg gggattctac tggtgtttgt tctaaagtta
     3721 ttcccctaca tttatatgta tactaaaggt gcgttaaaga aggttgatgc atctttggga
     3781 gaagctgcgg aaagtctcgg gtatcatgga ataaagaaaa tattaacggt cgatttgaaa
     3841 ttgatcactc cgacaatcct tgcaggtgca accgttgtct ttttgagagc tttttccgat
     3901 tatgggacac cgcggttaat cggtgaagga tatataactc tcccggtcat tatctaccag
     3961 gaatgggtca gtgaaaccgg tagtaacgca tattttgcat catctgtcgc gatgattatg
     4021 atgactgtcg cggcaattgt cttcttaatt cagcaacaaa tttccaacaa aaaaaactac
     4081 accatgagtt cactgaatcc gcctcaaccg aaacaactcc atgggattaa aaactttttt
     4141 gctcatttct ttgtatattt cattgtcttt ttggccacct tgcctcaaat ctatatcatc
     4201 accatttctt tccgaaataa caatgggatg atgtggacgg aaggttacgg attcggcaat
     4261 tatcaagcgg tatttcaaaa agcgacgaac tcaatcttga atacttttaa atttggaatc
     4321 atcgcgattt ttattgttat catcttcgga acactctttg cttatctgac ggttcgaaga
     4381 aaaaatatat tctcaaaaat tttagacgtc tttgtgatgt ttccgtacgt tattccgggg
     4441 acaatcttcg gtcttatttt attaatgact tataattccg gaccgattat tctgagcggg
     4501 accgccgcca tcatcattat ttcttatgtc attcgcagaa tggcgtatac cgtacggtca
     4561 agttccgcta ttttaaggca aataaatccg aatatcgaag aggcatcgat tagtttggga
     4621 tattcatcgc ttcccacctt tttcaatgtc acaatgccgg ctatggttcc ggggattatt
     4681 tcaggtgcga tcctaagttg gataacgatt attaatgaac tttcgtcaac tctgatgcta
     4741 tataccagca aaaccgccac aatgtcggtg gctatttttc aggaaattaa tcggggaggc
     4801 tatggaacgg cagcggcttt atcaacgata ttaacattta cgatggtggt ttctttactt
     4861 ctattctttc gatttacgaa atcagaggaa attgatttgt aatccacatt aataaatggg
     4921 aaagttgtgt ttgtaagcgt ttgccgaatc caatcattaa tccttagaga aactcacgaa
     4981 gagccgctgc aagcatctcc aaaccggcct gatcgt
//
LOCUS       DAOSMM010000097         4988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_16976_length_4988_cov_17.125887, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000097 DAOSMM010000000
VERSION     DAOSMM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16976_length_4988_cov_17.125887"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK898_09620"
     CDS             complement(<1..385)
                     /locus_tag="PK898_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20330.1"
                     /translation="MKRMIIWLGLIGLFNFSIVYAQNSEQEDPPVWVPVYEEVFSPAS
                     EGKGWIMGTDTSETTVINRSMVQDGYLWDITSPKHQYSIVQNSSLVIPEGYQIKVEAA
                     VKLPYYEPPVCSGIVFGADSDDFDTF"
     gene            complement(403..2871)
                     /gene="priA"
                     /locus_tag="PK898_09625"
     CDS             complement(403..2871)
                     /gene="priA"
                     /locus_tag="PK898_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU20331.1"
                     /translation="MNDLIAQVILNVPAKDGIFDYSIPEELIGKIKTGCMVVVPFRQT
                     YMQGIVLSLNDESAVSNLRPVTQLVDEMPVLSFAQLELAKKLSTRCLAPLGDCVNVFL
                     TEKIRKISEDNYTLVKKPDQKSISDEKPTEKERRKTLIREWLISLFPEENSVLAQSKI
                     DQKMEGENWKNAMDSLIREGVIRKDPVFSLPDNKLPVRRVAHLSATAETIESEWNALS
                     RQENALKRRQELLLCLKSTADGIPVTDLLRQTGATQVDIKALEKKHLISIAEEEAEGD
                     YTGLESDTYPGSEILLNAEQQKALEMIEQGLRATEQKVPILVHGVTGSGKTEIYIRAT
                     EQAIAQGKQVLVLVPEISLTPQILGRFSRRFPGKVGIYHSRLTDSQRYETWRKGRNGR
                     FRIVIGPRSALAVPLNDLGLIIVDECHDDSYYQIERQPYFSAVQAASDYAALSDAQLV
                     LGSATPTIAQVFKAQQSKWKIVRLKERASGISKPTVKLIDMRQELKSGNRSVFSRALI
                     EELKETLADEKQAILFLNRRGSAGYTFCHACGYDFRCPRCEIPLTWHQKQKKLCCHFC
                     GYTESLPEVCPRCGEKEIHQFGTGVEQVENLIRAIFPSASILRMDSETTAKKGSYERI
                     LSQFAERRADILIGTQMVTKGLDFPGVRLVGVLLADVGMNFHDYRVDEHTFQILTQVT
                     GRAGRVDVKGMAILQTYQPARYSIQAAVRGDFDSFYKKELAYRREIGYPPFSRLVRIV
                     ISDPLQSVTEKRAFDLSEEIRKILRTENHHATNLIGPAPCFFPKLDGKYRWHIILRGP
                     NPTDIIKKVDTKTVRVEVDPPSVL"
     gene            complement(3024..4910)
                     /locus_tag="PK898_09630"
     CDS             complement(3024..4910)
                     /locus_tag="PK898_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20332.1"
                     /translation="MAEQAEKKCQKTNKKKMSDWGSVLVILSIIEGCWSTATLFEIPS
                     EAGNAVFLGYSAERLAIAAICILGIIFLIGFFLLRKRGRYSVIIRQITFGGSALIFTL
                     CALIFLLNYSDIGQYDTLISSLVSRYGGIFVWGILFGIEIGATVVIDSKREDGGLNRL
                     LNPLYTAVLFGMAAVLFSLAFEYYDRVDWTRHMGHARAIFLAPAAVGILWALSEELIK
                     GKEAKSKANTVWLFLMIASLAFAVYRLTGFWIYRTDTPPKSYWDLLANAFLNRQLYLN
                     NPPTTHDLTMYNGKWYVPNPPLPAILLMPFVKWFGVEGINMTVVSAIIGALNTALIFL
                     ILQRAVQIGMIRCERSAILWLTVVFAFGTNHIWLAVTGQMWFISQLITVTMIELSVLA
                     VISKWSGWLVGLFFGFAILARPNIFPIAFLLLGIYLWEQADFPKADWRKTINWGIKTA
                     FPVLICLCLLLSYNKLRFDDWFDFGYVTINGAPWILEAVQKYGMFHPHFFQTNLRVML
                     WQLPTLDFSGDRFFFQPGISGFSIFVMTPPLIYLFRRFRKNWWVIGAWASVILTILLL
                     LFYHNTGAEQVGYRYLLDAIVPIMLLMGLGVDRKVNWFFKVLAMIGIAVNLLSVYWWF
                     IART"
BASE COUNT         1445 a         1244 c          990 g         1309 t
ORIGIN      
        1 aaaaagtgtc aaaatcgtcc gaatccgccc cgaaaacaat tccggaacaa accggcggct
       61 cgtagtacgg aagcttcacg gcagcttcaa ctttgatctg ataaccttcc gggatcacca
      121 aagatgaatt ttgaacaatc gaatactgat gttttggaga ggtaatatcc caaagatagc
      181 cgtcttgtac catagaacgg ttgatcacgg ttgtttcgga agtatccgta cccattatcc
      241 agccttttcc ttctgaagcg ggactgaata cttcttcata taccggtacc caaaccggcg
      301 gatcctcttg ttcactgttc tgtgcataaa caatcgaaaa attaaataac ccgataagtc
      361 ccagccaaat aatcattctt ttcataagtt tccatgcttt ctttataata ccgacggagg
      421 atcaacttca acccgaactg tttttgtatc cactttttta ataatatctg tgggattggg
      481 accgcgtaaa ataatatgcc aacgatattt cccatccaat ttcggaaaga aacacggtgc
      541 gggtccgatc agattggtcg catgatggtt ttcggtacga agaatttttc gtatttcttc
      601 cgataaatca aaggctctct tttccgtaac ggactgcagc ggatcggata tcacaatccg
      661 taccaatcgc gaaaacggcg gatatccgat ctcccgtcga taagccaatt cctttttata
      721 gaaagaatcg aaatctcccc gtaccgctgc ctgaatagaa tatcgagccg gttgataggt
      781 ttgtaaaatc gccattcctt tgacatcgac tcgacccgcc ctgccggtca cttgtgtcaa
      841 tatctgaaaa gtatgttcgt ctacacgata atcgtgaaaa ttcattccca catccgcaag
      901 gagaacaccg accaatcgaa cccccgggaa atccaaacct ttggttacca tctgcgttcc
      961 gatcagaata tccgcacgcc gttcggcaaa ctgagacaaa attcgttcat aagaaccttt
     1021 ttttgcggtg gtttcggaat ccattcgtag aatggaagcg gaagggaata tcgcacgaat
     1081 cagattttcc acctgttcga cacctgttcc gaattgatga atttcttttt ctccgcaccg
     1141 ggggcaaact tccggtaagg attcggtata cccgcagaaa tggcaacaga gttttttttg
     1201 cttttgatgc catgtcaacg gaatttcaca acgcggacaa cgaaagtcat acccgcaggc
     1261 atgacaaaaa gtataacctg ccgagccgcg acgattcaga aataggatag cttgtttctc
     1321 atccgcaagc gtctctttta attcttcgat taaagctcgg ctgaaaacag agcgatttcc
     1381 cgattttaac tcctgacgca tatcgatcag tttcacggtc ggttttgaga tgccggatgc
     1441 cctttctttt aaccgtacaa ttttccattt actttgctgg gccttaaaaa cctgagcgat
     1501 tgtgggggtc gcgctgccta aaaccaattg tgcatcgctg agagctgcat aatccgacgc
     1561 agcttgaacc gccgaaaaat aaggctggcg ttcgatttga taataagaat catcatggca
     1621 ttcatcgacg ataatcagcc ccagatcatt cagcggaacc gccaaagcgc ttctcggtcc
     1681 gataacaatt cggaagcggc cgttccgtcc ctttctccaa gtctcataac gctgactgtc
     1741 cgtcaatcga gagtgataaa tgccgacttt tccggggaaa cgtcgactaa agcgtcctaa
     1801 aatctgcggg gtcagtgaaa tctccggaac caagactaaa acctgttttc cctgtgcgat
     1861 cgcctgctcg gttgcgcgga tatagatttc tgtttttccc gaaccggtca caccatggac
     1921 cagaatcggc actttttgtt ccgttgctct caatccctgc tcaatcatct caagtgcttt
     1981 ttgttgctcg gcattcagca gaatttcaga gccggggtaa gtatccgatt ccaatccggt
     2041 atagtctcct tccgcttctt cttcagcaat cgaaatcaaa tgtttctttt caagagcctt
     2101 tatgtccact tgagtagctc ccgtttgccg cagtaagtcg gttaccggta tgccgtctgc
     2161 cgttgacttc aggcataaca ataattcctg ccttcttttg agagcatttt cctgccggga
     2221 aagagcattc cattcggatt cgatcgtttc agcggttgcc gaaagatgtg caacacgacg
     2281 aacaggaagt ttgttatcgg gtaaagaaaa aaccggatct tttcggatca caccctcccg
     2341 aatcaacgaa tccatcgcat ttttccagtt ttccccttcc attttttgat caattttaga
     2401 ttgagccaat accgaatttt cttccgggaa aaggctaatt aaccattcac gaataagcgt
     2461 tttccgcctt tctttttcag tcggtttttc atccgaaatg cttttttgat ccggcttttt
     2521 cacaagggtg taattatctt cggatatttt ccgaattttt tcggttaaga agacattgac
     2581 acaatcgccc aaaggagcta aacagcgcgt ggagagtttt tttgctaact caagctgtgc
     2641 gaaagataaa acaggcattt catccaccaa ctgagtaacc ggccgcaagt tcgatactgc
     2701 agactcatca ttcagagaaa ggacgattcc ctgcatatat gtttgacgaa acggaacgac
     2761 taccatgcat cctgttttga ttttcccaat cagttcttcc ggaatgctgt agtcaaaaat
     2821 tccatctttt gccggaacat taagaatcac ttgagcaatc aaatcgttca ttctataatt
     2881 ttaacccgag tgatccgcaa aaaattttcg gatgaagata accctgaaaa taaaataata
     2941 gaatcggaaa aaggaaaagg gctgaattga gtgccgaaat aaatatttcc ggcatagacg
     3001 aattcaggga aaggataatc cggtcaggtc cttgcaataa accaccaata aaccgacagg
     3061 agattcaccg caatcccgat catcgccaac actttgaaaa accaatttac ctttcgatca
     3121 acccctaagc ccatcaacag catgatgggc acaatggcat ccaaaaggta ccggtaccca
     3181 acctgctcag ctccggtatt gtgataaaac aatagcaata aaatggtgag aatgacagaa
     3241 gcccaggctc cgataaccca ccaatttttt cgaaaacgtc gaaacagata gatcaaaggc
     3301 ggagtcatta caaaaatgct gaaaccggat atgccgggtt ggaagaaaaa ccgatcaccg
     3361 gaaaagtcca gtgtcggcaa ctgccacagc atgacccgta aatttgtttg aaagaaatga
     3421 ggatgaaaca tcccatattt ttgaacggct tccaaaatcc aaggggcacc gtttattgtg
     3481 acatatccaa aatcaaacca gtcatcaaaa cgtaatttat tgtaagagag caacagacag
     3541 agacagatca gcacggggaa agctgtttta atcccccaat ttattgtttt gcgccaatct
     3601 gctttcggaa aatcagcctg ttcccaaaga taaatcccta ataataggaa tgcaatcggg
     3661 aatatattgg gtctggcaag tattgcaaaa ccgaaaaata aaccaaccag ccacccgctc
     3721 cattttgaaa tcaccgccaa aacggatagt tcaatcattg ttaccgtaat caattggctg
     3781 ataaaccaca tctgtccggt cacggccaac caaatatggt ttgttccgaa ggcaaaaacg
     3841 actgtcaacc aaagaatagc ggatcgctca caacggatca tcccgatctg aaccgctcgt
     3901 tgcaaaataa gaaaaatcaa cgctgtattc aaggcaccga tgattgcgga tactaccgtc
     3961 atgttaattc cttcaacgcc aaaccatttc acgaacggca tcaaaagaat tgccggcaag
     4021 gggggattcg gcacatacca cttcccgtta tacatcgtca gatcatgcgt tgtcggcggg
     4081 ttattcaaat acagttgcct gtttaaaaac gcatttgcca acagatccca gtagctcttc
     4141 ggcggagtat ccgtacgata aatccaaaaa ccggtcaatc gatatacagc gaaagccaac
     4201 gaggcaatca tcaaaaacaa ccagactgta tttgcttttg atttcgcttc ttttcccttg
     4261 atcaattctt cggatagcgc ccacaggatc cccactgccg ccggtgctaa aaatattgct
     4321 cgcgcatggc ccatatgacg agtccagtct acccggtcat aatattcaaa tgccagggaa
     4381 aacaggaccg ctgccatccc aaacagaaca gccgtataca gcgggttcaa cagcctgttc
     4441 agtcccccat cctccctttt gctgtcgatg accaccgtcg ccccgatttc tatgccgaac
     4501 agtatccccc atacaaatat ccctccatat cgggatacaa gagacgaaat taacgtatca
     4561 tattgtccga tatcgctata attcagaaga aatatcagag cacacagagt gaaaatcagt
     4621 gcgcttcccc caaatgttat ctgccgaata attacagaat atcgtccccg ctttctcaat
     4681 agaaagaaac cgattaggaa gattatcccc agaatacaga tcgctgcaat tgctaaccgt
     4741 tccgccgaat agccgagaaa aacggcattc cccgcttcag agggtatttc aaataatgtg
     4801 gctgtcgacc aacacccttc tattatggac aggatcacca aaacggagcc ccaatctgac
     4861 atcttcttct tattcgtttt ttggcatttc ttttcagctt gttctgccat gtccgtgtcc
     4921 ttcgagatta tccttcaggc tctgccgata taccaccagt aggtggagag caggttgatc
     4981 actacacc
//
LOCUS       DAOSMM010000098         4568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_18986_length_4568_cov_7.855750, whole genome shotgun sequence.
ACCESSION   DAOSMM010000098 DAOSMM010000000
VERSION     DAOSMM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18986_length_4568_cov_7.855750"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..3630
                     /locus_tag="PK898_09635"
     CDS             121..3630
                     /locus_tag="PK898_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOU20333.1"
                     /translation="MDKLENLKPNCQVKGILPNDTVTILSTQWYGSNIVNVTFRGTNG
                     ILGSELVFRDRENDLEIIAPGTVRSFDADPELFRLVSEAYRIRMAYLFDPWLAVHLSR
                     VEPLPHQITAVYGEMLPRQPLRFLLADDPGSGKTIMTGLFIKELFLRGDLQRCLIVTP
                     GNLTEQWQDELWQKFQLPFEIMTNDKLEAARTGNAFLETPLVIARLDKLSRDETIQTK
                     LSQTDWDLVVVDEAHKMSASVFGGEIKYTKRYKLGQLLSARTRHLLLLTATPHNGKEE
                     DFQLFMGLLDPDRFEGRYREGVHNIDVSDMMRRLVKEQLLKFDGTPLFPERRAYTVNY
                     NLSDQEAALYEAVTKYVSEEFNRADTLETNRKGTVGFALTMLQRRLASSPEAIYQSLK
                     RRRERLESRLREERLLKRGKEAQFDFAENIPVLDSESIEDLEDAPDDEQEQDTEQLID
                     LATASRTIQELELEIDELKRLEKMADQVRRSGNDRKWHELSLILQENDKMFDSDKHRR
                     KIVIFTEHRDTLNYLADKIRNLLGRSEAVVTIHGGMLREERRKVQNAFIQDKDVLILV
                     ATDAAGEGINLQRAHLMVNYDLPWNPARIEQRFGRIHRIGQTEVCHLWNLVAAETREG
                     DVYARLLEKIEEQRRVLGDGIFDILGKLFRETSLRDLLLEAIRYGDRPDVRDQLNHAV
                     DNLVDRKHCQELLEDRALARDSMDITQVQQIRQDFERAEARRLQPYFIETFFIAAFKH
                     LGGNIYPRKDRQYEISYVPSIIRNRSFETGRGTLLNRYERITFYKEESNQPGKPLAEF
                     LCPGHPLMDTVIDLILERYRSLLKQGAILIDQADSSELPRLLFYLEESICDGSEEAGG
                     KRHVVARQMQFIEISKDGEKTFAGPAPFLNYAPFPKEMRSLIEPILNEDWLKYNWEEI
                     AVSYAIQNIIPQMVRETKEQRDELLTKTLAAVHDRLIKEISYWDHRAQELKAQEQSGK
                     PNARLNSELAKRRADELETRLNNRCKELEQARCLSPQPPLLLGVALILPAGLISRLQG
                     QKTSTVDLFSKQKKAVEEAAMQAVFEKERELGFLPMDVHLENMGWDIESAIPDSGKLR
                     FIEVKGRIFGEETITVSKNEILAGLNKPEDYFLVIVEVVFEGEIAKAKDIHYVSRPFR
                     REPDFAVTSVNYKIKELLKQEKE"
     gene            3814..>4568
                     /locus_tag="PK898_09640"
     CDS             3814..>4568
                     /locus_tag="PK898_09640"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOU20334.1"
                     /translation="MPYRKKLIEVSLPLEAINRESAREKSIRFGHPSTLHLWWARRPL
                     ATCRAVLWASLIDDPSEYMPDEESADRERERLFSVLEQLVKWENIQNEKVLDAAKLEI
                     ARSIARDLGCEVPIGKNAINEFLAAKAPPVLDPFAGGGSIPLEAQRLGLRAFAGDLNP
                     VAVLINKAMIEIPPQFAGSAPVHPPEAGERQGSLLKKTWKGTEGLAEDVSYYGKWMLE
                     QAQKRIGHLYPKYQITAELLAERPDLRTQGLKAG"
BASE COUNT         1336 a          986 c         1094 g         1152 t
ORIGIN      
        1 atataattac tcataatcta tcgtaccgga ttcttgccgt cacagcaggg tgcgggaatg
       61 attaagtttt tttgatcaaa agaaataact gaatgcatca gacaggatcc aaaccttcat
      121 atggataaac ttgaaaatct aaaaccgaat tgccaagtaa aaggcattct gccgaatgat
      181 accgtcacta ttctcagtac tcaatggtac ggatcgaata tcgttaatgt tacgtttcgg
      241 ggaacaaatg gaattttagg gagtgagctg gttttccgtg atcgggaaaa cgatttggaa
      301 ataatcgctc cgggaacggt tcggtccttt gatgccgatc cggaattatt tcgtttagta
      361 tcggaagcat atcgcatccg catggcttat ttattcgacc cctggctggc cgttcatctt
      421 tctcgggttg aaccgcttcc gcatcaaatc acagcggttt atggagaaat gttgccccgg
      481 caaccccttc gttttttgtt ggcggatgat cccgggtccg ggaagacgat tatgacaggt
      541 cttttcatca aggaattatt tttaagaggt gatttacaaa gatgcttaat tgtcacgcca
      601 ggcaatctga ccgaacaatg gcaggatgaa ttatggcaaa aatttcagtt gccttttgag
      661 ataatgacta acgataagtt ggaagccgcc agaaccggca atgctttttt agaaacgcct
      721 ttggtaatcg cccgtttaga taaactcagc cgggacgaga cgattcaaac caagctctct
      781 caaacggatt gggatttggt cgtagtggat gaagcccata aaatgtcagc ttccgttttc
      841 gggggagaaa tcaaatacac taagcgttat aagttaggac agctcctctc agccaggacc
      901 cgtcatttac ttttgttaac cgctacgcct cataacggaa aagaagagga ttttcaactt
      961 tttatgggac ttttggatcc cgatcgattc gaaggaagat atcgggaagg tgtccataat
     1021 attgacgttt ccgatatgat gcgcaggctt gtaaaggagc aactccttaa atttgacggt
     1081 acaccgcttt tcccggaacg gcgagcttac accgttaatt acaatctatc cgatcaagaa
     1141 gctgcccttt atgaagctgt gacgaaatat gtttccgaag aatttaatcg tgcggatacg
     1201 cttgaaacga atcggaaagg gacggtcgga tttgcattaa ccatgttaca gcggcgattg
     1261 gcttccagcc cggaagcaat ctatcaatct ttaaagcgtc gccgcgaacg ccttgaaagc
     1321 cggttaagag aagagcgact tttgaaaagg gggaaggaag cccagtttga ctttgcagaa
     1381 aatattcctg tgttagattc cgaatcgatc gaagatttgg aagacgcacc cgacgatgag
     1441 caggaacaag atacagaaca actgattgat ctggcaacgg cttccaggac aattcaggaa
     1501 cttgagctcg aaatcgatga attaaaaagg ttggagaaaa tggcggatca ggtccggcga
     1561 tccggaaatg atcgaaaatg gcatgaactc tctcttattc ttcaagaaaa cgacaagatg
     1621 ttcgattcgg ataaacatcg tcgaaaaatc gtcatcttca ccgaacatcg agacacactc
     1681 aactatcttg ccgacaaaat tcgcaattta ttgggtcggt ctgaagctgt cgtaaccatt
     1741 catggcggaa tgttgcggga agaacgacga aaagttcaaa atgctttcat tcaagataaa
     1801 gacgtattga ttttggtggc aacagatgct gcgggtgaag gaattaattt gcagcgagct
     1861 catttaatgg ttaattatga tttgccatgg aatccggctc gtatcgagca acgtttcggc
     1921 cgtatccaca gaatcggcca aacggaagta tgccatttat ggaatttggt ggcggcggaa
     1981 acacgcgaag gagatgtcta cgcccgtttg cttgaaaaaa tcgaagaaca acgccgggta
     2041 ctcggagatg gcatttttga tattttaggt aaattattcc gcgaaaccag cctgcgtgat
     2101 ttattactgg aagcgattcg ttacggggat cgtccggatg tccgtgatca attaaaccat
     2161 gcggtggaca acctggtcga tcgtaagcat tgtcaagaat tgctggaaga tcgtgcgctc
     2221 gcgcgtgatt ccatggatat cactcaagtt cagcaaatcc gtcaagattt tgagcgtgca
     2281 gaagcccgtc gcttacaacc ttacttcatt gaaactttct ttattgccgc atttaaacat
     2341 ttaggcggca acatctatcc gcggaaagac aggcaatatg aaatctctta tgttccctcc
     2401 attattcgca atcgcagttt cgaaacgggg cgcggaacct tattgaaccg ctacgaacgg
     2461 ataacttttt ataaagagga atccaaccaa ccggggaaac cgcttgctga atttctctgc
     2521 ccggggcatc cattaatgga taccgtaatc gaccttatcc tggagcgtta tcgttcctta
     2581 ttaaagcagg gcgctattct aatcgatcaa gccgactcga gcgaactccc gagactcctt
     2641 ttctaccttg aagaatcaat ctgcgacgga agcgaagaag ccggtggaaa gcggcatgtg
     2701 gtcgctcgtc aaatgcagtt tatcgaaata tcaaaagacg gggaaaaaac ttttgcgggt
     2761 ccggctcctt ttttgaatta cgctcctttt ccgaaagaga tgagatcgct aatcgagcca
     2821 atcctcaatg aagactggtt gaaatataac tgggaagaaa tcgctgtatc ctatgctatc
     2881 cagaacatta ttccgcaaat ggttcgtgag accaaagaac aacgggatga attgttgaca
     2941 aaaacactgg cagccgttca cgatcgtttg atcaaggaga tcagttattg ggatcatcgc
     3001 gcccaggaac tgaaagctca agagcaatcc ggtaaaccga acgcccgttt gaactcggaa
     3061 ttggcaaaac gacgtgcgga tgaactcgaa acacgtctaa ataatcgatg taaagaattg
     3121 gaacaagccc gttgtttatc tccgcaaccg ccgctgcttt taggtgtggc actgatctta
     3181 ccggccggat taatcagtcg attgcagggg cagaagacat caaccgtaga tctgttctca
     3241 aaacagaaaa aagcggtcga agaagccgcc atgcaagccg tgttcgaaaa agaacgagaa
     3301 ctggggtttt tgccgatgga tgttcatctt gaaaatatgg gatgggatat cgaatccgca
     3361 attcccgaca gcggtaaatt acgattcatc gaagtaaaag ggcggatttt cggcgaagaa
     3421 acgatcactg tcagcaaaaa tgaaatttta gccggattaa ataagccgga agattatttt
     3481 ttggtgatcg tagaagtagt attcgaaggt gaaatcgcga aagcaaaaga cattcattac
     3541 gtttcccgtc cattccgccg ggaaccggat tttgcggtaa caagcgtcaa ttacaaaatt
     3601 aaagaactcc tgaaacagga aaaggagtaa aacgaataca ggattattga atatccggat
     3661 tcgtaatagg atattgtgct tttttaatag gggtttcggt tcaagaaaac cgttataatt
     3721 tattatgaac aatcgaaaga agccgtaagt tccttcctga ttttttaatt tttttaaatt
     3781 ttcggcaaaa tcaataaaat aaggaattaa ttcatgccat atcgaaaaaa actgatcgaa
     3841 gtatcgttac ctttggaagc cattaatcgg gaatcagccc gcgaaaagtc catccgtttt
     3901 ggacatccca gcacactcca tctctggtgg gcacgtcgcc ctttagcaac ctgccgggca
     3961 gtgttatggg cttccctaat cgacgaccct tcggaatata tgccggatga agaatccgcc
     4021 gatcgcgaac gggaaaggct tttctccgtc ctcgagcaat tagtcaaatg ggaaaatatt
     4081 caaaatgaaa aagtactgga tgccgcgaaa ctggaaatcg cccgcagcat cgcccgtgat
     4141 ctcggttgtg aggttcccat cggtaagaac gcaatcaatg aatttttggc tgccaaagca
     4201 ccgccggtgt tggatccgtt cgccggaggc ggctcgatcc cgttggaagc ccaacgcctg
     4261 ggcttgcgag cttttgccgg tgacctgaac ccggtagcgg ttttgatcaa taaagccatg
     4321 atcgaaattc ctccccaatt tgccggttcc gcccccgttc acccgccgga agccggcgaa
     4381 agacaaggat cccttttgaa aaaaacctgg aaagggaccg aagggttggc ggaggatgtg
     4441 agctattacg ggaaatggat gttggaacaa gcccaaaaaa ggatcgggca cctttatccg
     4501 aagtatcaaa tcaccgccga actgctcgcc gaacgaccgg atttacgcac acaaggcttg
     4561 aaagcggg
//
LOCUS       DAOSMM010000099         4533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_19202_length_4533_cov_18.889906, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000099 DAOSMM010000000
VERSION     DAOSMM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19202_length_4533_cov_18.889906"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..935
                     /locus_tag="PK898_09645"
     CDS             54..935
                     /locus_tag="PK898_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU20335.1"
                     /translation="MIFEKVSEHIWYMPREDETDRPALGYVNGGKTSLMVDSGNSPEH
                     ISYFYGVVRSMGMPDPGFVVLTHWHWDHVFGLTGSPAKSVAHVLTNERLQIMQRWDWS
                     DSSISARVSAGVETQFAFDMIRKEMPSRHSFKVKQADISFEVQCAIRLEDLECQLVHV
                     GGPHSDDSIVVYVPQEKVLFLGDCAYPDIYNNNSLRLSELSSLILRLEQFEADLFIPS
                     HSDPKSKNQFMSELKSLEKIGIAVGDLTDPALLPALLEKELGHQPTKEEIESAEHFVI
                     GNQLSNRNLMDHPFFFK"
     gene            1241..2173
                     /locus_tag="PK898_09650"
     CDS             1241..2173
                     /locus_tag="PK898_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOU20336.1"
                     /translation="MEYKDYYKILGVDKNASNEAIRKAYRDLAKKYHPDHNPGNKAAE
                     EKFKEINEAYEVLKDKDKRARYDQLGSSYSAWQQQGGNSANYNWSDWFSTAPGGQGTY
                     YATDFENMGDFSDFFSQIFGGMGSSRRSRTSRSTGFGQSYTPTKPAPVEYTLPITLKE
                     AFTGTERILTIGNKRIEAKIPAGAKTGTKVRLAGVMGEESGNADLILNIKVEDDPQFE
                     RVNDDLYVDVPIDLYTAILGGEVIVPSINEKIVLKIPAGTQPGQLIRMAGKGMPKLKG
                     KGTRGDLYARIKVQIPRNLTEKQKKLFEELRKEK"
     gene            2177..3832
                     /locus_tag="PK898_09655"
     CDS             2177..3832
                     /locus_tag="PK898_09655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOU20337.1"
                     /translation="MKQEELQFIPLGGLGEVGRNMMVFGYEDRYIIIDCGVQFPDNSM
                     IGVDYIIPDMQFLQGKEDKILGIIITHAHEDHIGGLPHLFDVLNNVPIYATPLTAGMI
                     EVRLARAGKLTKTKINVKKAGEEFDLGKFHIETFHMTHSIPDSIGLAITTPAGLIVVS
                     GDYKFDPTPIDNWPSDFAKLAELGTRNVLALFSDSTNSERPGTTPSEKDINKAFEEVF
                     VNARGRVIISSFASLISRLQQLSTIARRHHRKICIVGTSMVDNVNLAKRLGYLDLPDE
                     WVVTLEEALHLPDKEVLILCTGSQGEPTSIMGRLSTGKYYAFSIKEGDTVMLSSNPIP
                     GNEERVSAVINRLYRMGANVIYNSLLSVHVSGHACQEEMKLLINLVKPEYFIPVHGEL
                     RHLYNQAKIGELMGIPKNRIFVVENGQTIKFHHGKARLAEQVPASMVLVDGTNVGDMN
                     LDIMHQRERLADSGVIVITLMLDKDHHGLIRDPQIIHYGLMSGKETPDIEEDLRRTII
                     EYCGKKNSTGTNNIEKNIEQIVRKFIYNTTHRSPWVIVNPFYF"
     gene            4048..4120
                     /locus_tag="PK898_09660"
     tRNA            4048..4120
                     /locus_tag="PK898_09660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4081..4083,aa:Thr,seq:tgt)
     gene            4190..4272
                     /locus_tag="PK898_09665"
     tRNA            4190..4272
                     /locus_tag="PK898_09665"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4224..4226,aa:Tyr,seq:gta)
     gene            4358..4430
                     /locus_tag="PK898_09670"
     tRNA            4358..4430
                     /locus_tag="PK898_09670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4391..4393,aa:Thr,seq:ggt)
BASE COUNT         1400 a          899 c         1048 g         1186 t
ORIGIN      
        1 cagggataag ctaaaattgg atttgccatt agaacaggat taggtaggga aaaatgatat
       61 ttgagaaagt cagtgaacat atttggtaca tgccgagaga agatgagaca gaccggccgg
      121 ccctcgggta tgtgaatggg ggtaagacca gtttaatggt tgattccgga aattcaccgg
      181 aacatatctc ttatttttac ggcgttgtac gaagcatggg aatgcccgat cccggatttg
      241 ttgtactgac tcattggcat tgggaccatg tttttggatt gacgggatct ccggccaaat
      301 ccgtcgccca tgtcctgaca aatgaaagat tgcaaatcat gcaacgctgg gattggtcag
      361 acagctccat cagtgcccgt gtgtcggcag gcgttgaaac acaatttgcc tttgacatga
      421 ttcgaaagga aatgccttca agacattctt ttaaagtcaa acaggcggat atttcttttg
      481 aagttcaatg tgcgattcga ttggaagatc tcgaatgtca gttggtacat gtgggaggac
      541 cccacagcga tgattcgatc gttgtttatg ttccccagga aaaagtcctg tttttgggtg
      601 actgtgcata tccggatatt tataacaata acagtctgcg cttgtccgaa ttgagttcct
      661 taatcttacg attagaacaa tttgaagctg acttgtttat tccttcccac agtgacccga
      721 agagtaaaaa ccaatttatg tccgaattga agtccctgga aaagatcggt attgcagtcg
      781 gtgatctgac agatcccgcc cttctgcctg ctttactcga aaaagaattg ggacatcagc
      841 cgacaaaaga agaaatcgag tcggcggaac attttgttat cgggaatcaa ctttccaatc
      901 gaaatttgat ggatcatccg tttttcttta aataaaggtt agctgcagga attcatcgca
      961 aaaaaggttg cttttcgatt aaaacccata ataaatcggt tttccgtaat tgtttttaat
     1021 cgaatccgca tgaaatgatc ggcgaacatt ctgaaatttt gaagaataac ccctctgttt
     1081 gtattttgat ttccatataa tttataaaaa tcaaataatc cgattgtaaa atatggtaga
     1141 aatggagata tgtgtaaaaa aatctctgct taaagacctg tttctattga caaaccgatt
     1201 tgaatgattg ggataggaat gaaacagaaa gtggaggagt atggaatata aagattatta
     1261 taaaatactc ggcgtagata aaaatgcgtc gaatgaagca attcgcaaag cctatcggga
     1321 tttggctaag aaatatcatc ccgatcataa tccgggaaac aaagctgcgg aagagaaatt
     1381 taaagagatt aatgaagctt acgaagtttt aaaagataaa gacaaaagag cgcgttatga
     1441 ccaattgggc tcgtcttaca gcgcttggca gcaacagggt ggcaatagtg cgaattacaa
     1501 ttggagcgat tggttttcaa ccgcaccggg cggacaaggg acttattatg ctaccgattt
     1561 tgagaatatg ggcgatttct ccgacttctt ctcccaaatc ttcggtggaa tgggctccag
     1621 ccgccggtca aggacctctc ggtcaacggg attcggccaa agttacactc cgaccaaacc
     1681 cgctccggta gaatatacgc ttccgattac cttaaaagaa gcttttaccg gtacagaacg
     1741 tatcctgacg atcgggaaca aacgcattga agcgaaaata ccggccggtg caaaaaccgg
     1801 gacaaaagtc agactggccg gagtaatggg tgaagaatca ggaaatgcgg atctgatact
     1861 gaacatcaaa gtggaagatg acccgcagtt cgagcgtgtt aatgatgatt tgtacgttga
     1921 tgtgccgatc gatttatata ccgcaattct gggtggagaa gtgattgttc catccatcaa
     1981 tgaaaaaatc gtattgaaaa ttcccgccgg aacccaaccc ggtcaattaa tccggatggc
     2041 gggaaaagga atgccaaagt tgaaaggcaa aggaacgcgc ggtgatttat atgctcgaat
     2101 caaagtgcaa atcccgcgca acttgactga gaaacaaaaa aagttatttg aagaattaag
     2161 aaaagagaaa taaagaatga agcaagaaga actgcaattt attccgctcg gcggattagg
     2221 tgaagtcggg cggaatatga tggtattcgg ctatgaagac cgatatatca tcatcgattg
     2281 cggcgtgcag tttccggata attccatgat cggagtagat tacatcattc cggatatgca
     2341 atttctgcaa ggaaaagaag acaaaatcct cggaattatt atcactcatg ctcatgagga
     2401 tcatatcggc ggattaccgc atttattcga tgtattgaat aatgttccga tttatgcaac
     2461 tcccttgaca gcgggaatga tcgaagtcag gttagcccgt gccggaaaac tgacaaaaac
     2521 aaaaattaac gttaaaaaag ccggagaaga gtttgatttg ggaaagtttc atatcgaaac
     2581 ttttcatatg acgcattcga ttccggattc aatcggtttg gccattacca ctccggcggg
     2641 tctgattgtc gtcagcgggg actataaatt cgatccgaca ccgattgata attggccttc
     2701 ggatttcgcg aaattagccg agttgggaac acgaaatgta ttggctttat ttagcgattc
     2761 tactaactct gaacggcccg gaacaacccc ttcggaaaaa gatatcaata aagctttcga
     2821 agaagttttt gtcaacgcaa gaggtcgggt cattatttcc agttttgcat cattaatttc
     2881 ccgcttgcaa caactttcca caattgccag gcgacaccat cggaaaattt gtattgtcgg
     2941 aaccagtatg gtggataatg tcaacttggc aaaaaggtta ggctacttgg atttgccgga
     3001 cgaatgggtc gtaaccctcg aagaagctct acatttacct gacaaagagg tactgatcct
     3061 ttgcaccggt tctcagggcg aacccacctc tattatggga agactctcca ccggaaagta
     3121 ctacgctttc agcatcaaag aaggcgatac cgtaatgctt tcctccaatc cgatccccgg
     3181 aaacgaagaa agggtttcgg ctgtaattaa ccgattgtat cgaatgggag caaatgtcat
     3241 ctacaattcg cttctctccg tccatgtctc cggacatgcc tgccaggaag aaatgaaatt
     3301 gctgatcaat ttagtgaaac cggaatattt cataccggtt cacggtgaac ttcgccatct
     3361 ttataatcag gcaaaaatcg gtgaactgat gggaattccg aaaaaccgca tctttgtcgt
     3421 ggaaaatggg caaaccataa agtttcatca cggaaaagct cgattagccg agcaggttcc
     3481 cgcctccatg gttttggtgg atggaacgaa cgtcggagat atgaatctcg atatcatgca
     3541 ccagcgggaa cgcctggctg acagcggcgt catcgtaata accttaatgt tggataaaga
     3601 tcaccacggt ttaattcgcg atccgcaaat tatccattac ggcttaatgt ccggaaaaga
     3661 aactcccgat atcgaagaag atctgcgaag aacaattatc gaatattgcg gtaaaaagaa
     3721 ttcgaccgga acgaataata tcgaaaaaaa tatcgagcaa attgttcgga aatttattta
     3781 caatacgaca catcgctcgc cctgggtgat cgtaaacccg ttttatttct gaaatataca
     3841 cagaaatcaa tccgtttttt tctgtcaatg gatgcaaatg acagaaaaaa acggtaggta
     3901 acgatttcca caatatatca accgaattgg ttgaatttta tgataaactg gtcttcgttt
     3961 gtgccgcgaa cactttgatt ttcgtgtcct tgccatgcga ttcattgtct ggtataatag
     4021 cgaacgcgaa atgcacatta acagttcgcc gacatagctc aattggcaga gcagccgcct
     4081 tgtaagcggc aggtcatggg ttcaagtccc attgtcggct cagttgcaag gcagtaaatg
     4141 ggtttgaaaa aatcttcaaa tccatttacg gacaggcaac tgttcaaacg gacggttacc
     4201 caagtggcca acggggtctg actgtaaatc agatagcaga agctttcgga ggttcgaatc
     4261 cttcaccgtc cactgataat tcggaagaaa aaagaaaatt tttttcgtcc gaatatcaaa
     4321 agaaattttg catcggcgaa gttgctgaaa caaataagcc ctcatagctc agttggcaga
     4381 gcacagtctt ggtaagactg gggtcatggg ttcaaatctc attgagggct ctttttgatg
     4441 aatgggcata atcgtccgga taatgaggga gaataaaaat ggcaaaacag cattatgagc
     4501 gaaagaaacc acatgtgaat gtggggacca tgg
//
LOCUS       DAOSMM010000100         4393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_19961_length_4393_cov_15.771093, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000100 DAOSMM010000000
VERSION     DAOSMM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19961_length_4393_cov_15.771093"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..2083
                     /locus_tag="PK898_09675"
     CDS             143..2083
                     /locus_tag="PK898_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20338.1"
                     /translation="MPGTVTPEKKSIESILTEMAISTQQAITASDTIAPVTEIEEPEP
                     TIDPSKVLNVCLGSEPSSLFIYNNSSKSGWSVLESIYDGPFDTIDGRDVPVIFENVDF
                     VKDEVQAKEGDIVMNSRGEAVALAAGMEVTPANGENVCGSDACTVQWDGVTPLSFYQA
                     TITYKIKSGILWSDGETLSAKDSVYSWRLAGDPAIPGSKKMFNLTKSYKATDDQTVVW
                     QGVPGFIPQKASDVFWLPLPEHVMGNMKAADILVDEGINRTPIGWGAYKIAEWNPGDQ
                     ITVVRNASYRNTETGVALFFDKVVFHFYGKAGDNNLAALKSGSCDIVDTSVDLLPNLE
                     PILEDVRDGKISAYIQPQTVWEQLTLNFAPLNQKGFGYFADVGVRKGIARCLDREALI
                     RQVLYGQSEIPQGFYPSQHSLFVGNLTDTEFDPVAGRALLEDAGWIDSDGDSTTPRVS
                     SGVEGVPDGTEFAVTLETSESTERIKAAELIRDQLADCGISVTVQAEDQAKIYAQGPE
                     GSVFGRKFDMAMFAWASGDQSPCSLYSSSQIPTEENQWIGLNVGGYRSAEYDSACNIA
                     QNASPMEEDYLGKAEKVQQLFAEDLPVIPLYFYPAIGLSGNRICGLKNSIGMRSLLYN
                     IELLTRSDSNCAVSQWKNIYSE"
     gene            2148..2372
                     /locus_tag="PK898_09680"
     CDS             2148..2372
                     /locus_tag="PK898_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOU20339.1"
                     /translation="MEHKKLYRSQKDRFIAGVCGGLAKYFGIDATIVRVAWIVLIFTT
                     GFIPFVLIYILCALIIPLEPTSPKDVTIDQ"
     gene            complement(2454..3743)
                     /locus_tag="PK898_09685"
     CDS             complement(2454..3743)
                     /locus_tag="PK898_09685"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOU20340.1"
                     /translation="MNNEEINPFELAQKQFDHVADMLELPEDVRSYLRWPRKEFHVRV
                     PLRMDNGELKLFNGYRIQHNNALGPNKGGLRFYAGETVDTVRALATWMTWKCAVANIP
                     LGGGKGGINVDPSTLSEAEKERLIRGYVRLLWKNFGPRVDIPAPDMGTNAQMMCWFMD
                     EYSQQMGQYVPGVVTGKPVGAGGSLGRNSATGYGVVVCIREAMKRLGYNPKGMTASIQ
                     GFGNVGQYSATNFVEMLGGIVKCVSCWDRNDKKSYTYYKKEGVDPRFLMSITDQYGMI
                     NKGKAQEAGYEILDGDAWLSMDVDVLIPAALEGQIRADNVEKISPKLKILAEAANGPT
                     MTEADEVIKNRGYFLIPDFLCNSGGVTCSYFEGVQNDANFYWSEEEVENKLDDKMTIA
                     FNSVYQMAEDRKVYMRDAAYMVAIQRVVTAMRMRGWV"
BASE COUNT         1189 a          931 c         1028 g         1245 t
ORIGIN      
        1 cattcttatt tatagtatga cgcctaaggt ataatcattt tggattgtta ttcgactgaa
       61 acagggaaac cgaatgaaac ggattttatt tctttttatt tttatattat gttttgtagg
      121 ttgtaatacg ccggaatctc caatgccagg aactgttacg ccggaaaaga aaagtattga
      181 atccatatta acggaaatgg caatttcaac ccagcaagcg attactgcaa gcgatacaat
      241 cgcacctgtt accgaaatcg aagagccgga gcctacaatc gatccttcaa aagtattgaa
      301 tgtctgcctc ggaagcgaac caagtagtct gtttatctat aataattctt caaaatccgg
      361 ttggtcggtc ctcgaaagca tatatgacgg accgtttgat acgatcgacg gtcgcgatgt
      421 tcctgtaatt tttgaaaatg tcgattttgt aaaagatgaa gtccaggcaa aggaaggcga
      481 tatcgtgatg aattccagag gagaagcagt cgcccttgcc gctggaatgg aagtaactcc
      541 ggccaatggg gaaaatgtct gcggatccga tgcttgcacg gttcaatggg acggagttac
      601 gccactgagc ttctatcaag cgacgattac atataaaata aaatccggta ttttatggtc
      661 ggacggtgaa acattatcag caaaggattc ggtttattca tggagactgg ccggtgatcc
      721 ggcaattccc ggttcaaaaa aaatgttcaa cctgacgaaa agctataagg cgactgatga
      781 tcaaacggtt gtctggcagg gagttccggg ttttattccg caaaaggctt cagatgtgtt
      841 ctggttgccg ttgcccgaac acgtgatggg aaatatgaaa gcggcggata tcctggttga
      901 cgaaggaatc aaccggacac cgatcggttg gggtgcttac aaaatagcgg aatggaatcc
      961 gggggatcag ataaccgttg ttcgaaatgc gtcatatcgg aataccgaga ccggtgtggc
     1021 tctttttttt gataaggtgg tatttcattt ttatggaaaa gccggtgaca ataatctggc
     1081 ggcattaaaa agcggcagct gtgatattgt tgatacgtct gttgatcttc tgccgaattt
     1141 agaaccgatc cttgaagatg ttcgggacgg aaaaatttcc gcatatatcc aacctcaaac
     1201 agtgtgggaa caactcacac tgaactttgc tcctcttaat caaaaagggt tcggttattt
     1261 tgccgatgta ggcgttcgaa aagggatcgc ccgctgtttg gatcgcgaag cactgattcg
     1321 acaggtttta tatggtcaga gtgaaattcc tcaggggttt tatccttcgc aacacagcct
     1381 gtttgtcgga aatctgactg ataccgaatt cgatccggta gcaggtcggg ctttgctgga
     1441 agatgccgga tggatcgatt cggacgggga ttcgactaca ccgcgtgtct cttcgggtgt
     1501 ggagggggtt ccggacggaa cagaatttgc cgtcacttta gaaacctccg aatctaccga
     1561 aagaatcaaa gcggctgaat taattcgtga tcaattggcg gattgcggaa tttcggtaac
     1621 cgttcaagca gaggatcagg cgaaaatcta tgcgcaggga ccggaaggat ctgtatttgg
     1681 ccgaaagttt gacatggcaa tgtttgcttg ggcaagcggc gatcaatctc cctgttccct
     1741 ttactcgagc agccaaattc cgaccgagga gaatcaatgg atcggtctaa atgtcggcgg
     1801 ttaccgatct gccgaatatg attcagcttg taatatcgct cagaatgctt ctccgatgga
     1861 agaagattat ttaggtaaag ccgaaaaagt tcaacaatta tttgcagagg atttaccggt
     1921 aattcctctc tatttttatc cggctatcgg attgtccggt aatcggatct gcggactgaa
     1981 aaacagtatc ggaatgcgca gtcttcttta taacattgaa ttgcttacgc ggagcgattc
     2041 aaactgtgcg gtctctcaat ggaaaaatat ctattccgaa tgataatcgg aatatttcga
     2101 aacaatctgg cgataaatac gtatatcagt tagacaggag aaaaaacatg gaacataaaa
     2161 aattatatcg aagtcagaaa gatcgattta tcgcaggtgt atgtggcggt ttggccaagt
     2221 atttcggaat tgatgcgaca atcgttcggg tggcgtggat cgtattaata ttcacgacag
     2281 gttttattcc ctttgttttg atttatatcc tttgcgcatt aattattccg ttggaaccaa
     2341 cctcgccaaa ggatgtcaca atcgatcagt aattcataaa aagcggattg gttataaaaa
     2401 agcctattgc cgaatggcaa caggcttttt ttcatcaagc aattatgagg gctctatacc
     2461 catccgcgca ttctcatggc ggtaacaacc cgttgaatcg caaccatgta agccgcgtcg
     2521 cgcatatata ctttgcggtc ttcggccatt tgataaacgg aattaaaggc aatcgtcatt
     2581 ttgtcatcca gtttattctc gacttcctct tcgctccaat agaagttggc atcattttga
     2641 acaccttcaa aataggagca ggtcacgccg cccgaattac acagaaaatc gggaatcagg
     2701 aaataaccgc ggtttttaat cacttcgtct gcttcggtca tggtgggtcc gttggccgct
     2761 tcggcaagga tttttagctt cggactaatc ttttcgacat tatccgctcg gatttggcct
     2821 tcgagagcag ccggaatcag tacatctaca tccatactga gccaggcatc tccgtccagg
     2881 atctcatatc cggcttcttg ggccttccct ttattgatca ttccgtattg atcggtgata
     2941 ctcatcaaaa atcgcggatc cacaccctct ttcttgtaat aggtgtagct tttcttatca
     3001 ttgcgatccc agcaggaaac gcatttgacg ataccgccca acatttccac aaagtttgtg
     3061 gcactatatt gaccgacatt gccgaaacct tgaattgatg cggtcattcc tttgggattg
     3121 tagccgagtc ttttcatcgc ttctcgaatg cagaccacaa cgccatatcc cgtagccgag
     3181 tttcttccga gtgacccgcc ggcgccgacc ggtttaccgg tgactacgcc gggaacatac
     3241 tgtcccattt gttgagaata ttcatccatg aaccagcaca tcatttgtgc gttggtgccc
     3301 atatccggtg cgggaatatc cactctcggt ccgaaattct tccagagcaa acggacataa
     3361 ccgcgaatca ggcgttcttt ttccgcttca ctgagcgtgg aaggatccac attgattcca
     3421 cctttcccgc cgccgagcgg gatattggca acggcgcatt tccaagtcat ccaagttgcg
     3481 agagcgcgga cggtatcgac cgtctctccc gcataaaatc gtaaaccgcc tttattagga
     3541 cctaacgcat tgttatgttg aatacgatag ccattaaaca gcttcagttc gccgttatcc
     3601 attcgaagcg gaacacggac atgaaattct ttccgtggcc agcgtaaata cgagcgaacg
     3661 tcttccggca attcaagcat atccgcgaca tggtcgaatt gtttctgagc taattcaaac
     3721 gggttgattt cttcattgtt catccttaac gatctccttt tacagcatta aataattatg
     3781 atttgatttg tttgatataa gtttatattc gttttctgat aagagtaatt gatagattgc
     3841 cgataaatgt cagacattta tcacttgtca gtataccata tctgccgaat tctttttgct
     3901 tgatctgtca caaataaaat tatagaatag ataaaatttt tccataagga aaataaatag
     3961 taataaattt tttgaaaaaa tcgaataaat aattgtccgt atgacttatt ctccatatgg
     4021 ttttgtaaaa ttaaggtgct tttgtggtaa aaggaaattg tgaaaataaa aacaaatact
     4081 ttccataaag caaagattct gccggaagcg ttagtcgttg gcaaggagtt tcggcgcggg
     4141 aagtctgcac ctcgtgatct cggtgagctt cgcaaggatg ccgttctatt gagttggttt
     4201 tcccttgccc ttggggagag ggttagggtg agggaggatt cggagcggga cgctgtcccc
     4261 tgcaaaatat tcggtgcact ccgcaaggat gccgccccat cgagccggtt tcccctcgcc
     4321 ctttgggaga gggttagggt gagggaataa aattgatctt tgttttgttc aaacacggga
     4381 cataggagat gtc
//
LOCUS       DAOSMM010000101         4313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_20455_length_4313_cov_17.628699, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000101 DAOSMM010000000
VERSION     DAOSMM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20455_length_4313_cov_17.628699"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..946
                     /locus_tag="PK898_09690"
     CDS             95..946
                     /locus_tag="PK898_09690"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOU20341.1"
                     /translation="MAVIHEANRKFDRKNIYTKWILPDDQPIANIILVHGIGEHCARY
                     DHVTAFFADHHIAVYTFDQIGHGKSEGKRGAMTYDQTWGIINEIKAELIQKEPAIPVF
                     LYGHSMGGAIVLSYAYRYPENLTGVIATAPAIGLANPTSGFMTAMARFLSKIVPNMTI
                     SNGLALDGLTHDKKIVELYQNDPLVHDKVSFKLAVDLIDKGNEILKSADKFPVPLLLL
                     QGTEDKLVDPKATAQFAAHLNQNGTYKPIEGGYHELHNEPYKEEIFNIILQWINDRVN
                     EPEKENA"
     gene            1134..4259
                     /gene="gcvT"
                     /locus_tag="PK898_09695"
     CDS             1134..4259
                     /gene="gcvT"
                     /locus_tag="PK898_09695"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOU20342.1"
                     /translation="MENYFFNGDLTDIDNAIARLTKLEAERQKRKLIMIPSESFAPQA
                     VRESLGSVFQNVYAEGYPPEEMRSLTENEILDYKARLGDYRRNSDPRYYKGVEYIDIL
                     ESLARRRCAELFANDRISADEIQVNVQPLSGAPANNAVYHLINPGDTVLAMDLLHGGH
                     LSHGSPVNRSGMLYNIVHYTVNPETQLLDYDEMQRLASEYKPRMVIAGYTSYPYIPDW
                     ERYRKICDSVGAILMADIAHIAGLVAAGVVASPVGYADVITFTSHKTLTGPRAAVILT
                     TDPELSKRIDKAVFPGEQGGPHTQVFAALATNFKIAATPEFKVYQQQILNNTQAIIKQ
                     LQSRGIKVAYQGSNSHMLLVDCKCIKGEDGTTLSGDMAARILDNAGIVLNRNTIPGDK
                     SALRASGIRMGTSCITQIGLKEEESAQVADIIADLLFAIHPYSMPGAKGLLQRAKIDF
                     DVFESCKTRVRRLCEKHNKFTNSDEFGYPHFYYIDDSFVSEQGTSYLLISGDKSQEFV
                     NFAFQNDAESLSLNECQDAVISVNGELINGCLMLREDGYQFGCPSEKINVVAAWLRDL
                     SDGFIRFDDDLRMKLPGPVFISETIGIKGSESVISEFEKPYAIGKKRKEGIGLPEFDF
                     SVYENQETQPSKLTPLHDIHIALGAKMMPFAGWDLPLWYTSIKEEHQAVRNAAGLFDV
                     SHMGVYQVSGKDACAFLDSVCGNDISALEVGDTCYTHFLDLDANVIDDLIVYAIEPQK
                     YLVVVNASNDDKDWAWLNAVHNDEILIDRESPLSRAFGRNSILENLRSPEAGERMRVD
                     LALQGQASKRILAALDAAPEDRKKIDKLEKGHVTHVNLAGFDLYIARTGYTGEKISYE
                     LFVHPAKLPELWNMLLEAGKADGLMPCGLGARDSLRIEAGLPLYGHELAGPMNYRVNE
                     AGFSHFVKTYKPWFIGRNAYLKHVKEQKQEIVRFKFNEKAVRMAHTNDPVLDSKGRVI
                     GRVTSCSVDKDGWIIGMAMIESAYKQEGTSIFIYQNSEKLELKDPSSLQPGDRTVIPS
                     QATIISRYPKL"
BASE COUNT         1235 a          952 c          993 g         1133 t
ORIGIN      
        1 tttcaaatct cctcccttcg aaaacttgtt aaaacaagat ttcttcgcta tacttttatt
       61 atagaaaaac tcagaaatat ccaggaggca taaaatggct gtcatccatg aagcaaaccg
      121 caaatttgac cggaagaata tctatacgaa atggattctt ccggacgatc aacctattgc
      181 gaatatcatc ttggtccacg gtatcggaga acactgtgct cgatacgacc atgtcaccgc
      241 ctttttcgct gaccatcata ttgccgtcta taccttcgac caaatcggac acgggaaaag
      301 cgaagggaaa cgcggagcga tgacctacga tcaaacatgg ggaattatca atgaaataaa
      361 agcagaattg atccaaaagg aaccggcaat tcctgttttt ctctacggac attctatggg
      421 aggcgcaatt gttttatcat atgcctatcg ctatccggaa aacttaacag gcgtaatcgc
      481 gaccgctccc gcaatcgggt tggcaaaccc cacctcaggt ttcatgacgg caatggccag
      541 gtttctctca aaaatagtcc cgaatatgac catttcgaac ggactggcac ttgacggatt
      601 aacacacgac aagaaaattg tcgagctgta ccaaaacgac ccattggtcc atgacaaagt
      661 ctcttttaaa ttggctgtcg atttgatcga taaaggcaat gaaattctga aatccgccga
      721 caaattcccc gttcctttgt tattactgca agggaccgaa gacaagttgg tagatccgaa
      781 agcgaccgcg caatttgcag cccatctcaa tcaaaacggt acttataaac ccatcgaagg
      841 cggctaccat gaactgcata acgagccata taaagaagaa atctttaaca tcattttgca
      901 gtggatcaat gacagagtaa acgaacccga aaaggaaaat gcttgaatcg attttccgct
      961 tcatatgaga agaacggttc aatctgtcct tgaatttgcg gtagaaaatt ctcgagcgca
     1021 gcttttcggg aatcataatt ataagaattc gaaagatgtg ccgagaaata acggtagaat
     1081 taaatcatct ttaacaattc catcacaacg gatcctttgg ggaggcatta tccatggaaa
     1141 attatttttt taacggtgac ctcaccgaca ttgacaacgc gatagcccga ttaacaaaac
     1201 ttgaagctga aaggcaaaaa agaaaactaa taatgattcc cagtgaatcg tttgctccgc
     1261 aagctgtcag ggaatccctc ggttccgttt tccaaaatgt ttacgccgaa ggttatccgc
     1321 ccgaagaaat gcgttctctg actgaaaatg agatcttaga ttacaaggca agattaggtg
     1381 attatcgcag aaattctgat ccgcggtatt acaaaggagt cgaatatatt gatatcctgg
     1441 aatcgcttgc ccgccgcagg tgcgcggaac tttttgcgaa tgatcgcata agtgccgatg
     1501 agatccaagt caatgttcag ccgctgtccg gcgctccggc aaacaatgcg gtctaccatt
     1561 taattaatcc cggcgatacg gttttggcga tggatttgct tcacggcgga cacctttctc
     1621 atggcagccc ggtcaatcgc tccggcatgc tctacaacat tgttcactac acagtcaatc
     1681 ccgaaactca attattggac tatgacgaaa tgcaacggtt ggcatcggaa tataaaccgc
     1741 ggatggtcat tgcaggatac acttcttatc cttacatccc cgattgggaa cggtatcgaa
     1801 aaatatgcga cagcgtcgga gccatactga tggccgacat tgctcatatc gccggattgg
     1861 tcgccgcggg tgtcgtcgct tcaccggtcg gatatgcaga tgtcattacc ttcacctctc
     1921 ataagacttt aaccggaccg cgcgctgccg tgatcttaac caccgacccc gaactttcca
     1981 aacggattga taaggcggtt ttcccgggag aacagggggg acctcatacc caagtctttg
     2041 ccgcgttggc gacgaatttc aaaatcgctg ccacaccgga attcaaggtt taccagcaac
     2101 aaattctaaa taatacccag gctattatta aacaattgca gtctcgcgga atcaaagtgg
     2161 cgtaccaggg ctcaaacagc catatgcttt tggtggattg caaatgcatt aaaggggagg
     2221 acggtactac tctatccggt gatatggcgg ctcgtatctt agacaatgcg ggtattgttt
     2281 tgaatcggaa tacgatcccc ggagacaaat cagctcttcg cgccagcggc attcgcatgg
     2341 gaacatcctg tattactcaa attggtttga aggaagaaga atccgctcag gtggctgata
     2401 ttattgccga tctgcttttc gcgattcacc cttattcgat gccgggagca aaaggattgc
     2461 ttcaaagggc taaaatcgat ttcgacgttt tcgaatcttg taaaactcgt gttcgacgct
     2521 tatgtgaaaa gcacaataag tttaccaatt ccgacgaatt cggttatccc catttctatt
     2581 acatcgatga ttcgtttgtg tctgagcaag gaacctccta tctgttgatc agcggcgaca
     2641 aaagtcagga atttgtcaat ttcgcctttc aaaatgatgc cgaatcgctg tcgctgaatg
     2701 aatgtcagga tgctgttatc tctgtcaacg gtgagctcat taacggttgt ttgatgctga
     2761 gagaagatgg atatcagttc ggttgtccgt cggaaaaaat taatgtggtt gccgcttggt
     2821 tgcgggatct ttcggatggt tttattcgct tcgatgatga tttgagaatg aaacttccgg
     2881 gccctgtatt tattagtgag acgatcggaa tcaagggcag tgagagcgtg atcagtgaat
     2941 tcgaaaaacc ttatgcgatc gggaaaaagc gcaaagaggg aatcggttta cccgaatttg
     3001 atttttctgt ctatgaaaat caagagaccc aaccgtcgaa actcacgccg cttcatgata
     3061 ttcatatcgc tttaggcgcg aaaatgatgc ctttcgccgg ttgggatttg cccctttggt
     3121 atacctcgat caaagaagag catcaagcgg tacgcaatgc cgccggatta tttgatgtgt
     3181 cgcatatggg tgtttatcag gtttcgggga aagacgcctg cgcctttttg gacagcgtat
     3241 gcggaaatga catttcagca ttggaagtcg gagatacttg ctatacacat ttcttggatt
     3301 tggatgcgaa tgtaatcgat gatctcatcg tctatgcgat cgaaccgcag aaatatttgg
     3361 ttgtggtcaa cgcatcgaat gatgacaaag attgggcttg gttgaatgcc gttcataatg
     3421 atgaaatatt aattgatcga gaatcccctc tctcacgggc attcggccga aacagcatat
     3481 tggaaaatct tcgcagtccg gaagcggggg aaagaatgcg cgtggattta gctttacaag
     3541 gtcaggcttc caaacggatt ttagccgcgc tcgatgccgc tccggaagat cggaaaaaaa
     3601 tcgacaaact ggaaaaaggt catgtcaccc atgttaactt agccggattt gatctctaca
     3661 ttgcgcgcac cggttatacg ggagaaaaaa tttcttatga actttttgtt catccggcaa
     3721 agcttcccga actttggaac atgctgcttg aggccggtaa agctgatggt ttaatgcctt
     3781 gcggtttggg tgctcgtgat tccttgcgaa tcgaagccgg attaccgctt tacgggcatg
     3841 aattggccgg tccgatgaat taccgtgtca atgaagcggg tttttcccat tttgtaaaaa
     3901 cctataaacc ttggtttatc gggcgcaacg cttatctgaa acatgtaaaa gaacaaaagc
     3961 aggaaatcgt gcgcttcaaa ttcaatgaaa aagccgttcg aatggcacat accaatgatc
     4021 cggttttgga ttcgaaggga agggttatcg gtcgcgtcac cagttgttcg gtggataagg
     4081 atggctggat tatcggaatg gccatgatcg aaagcgccta taaacaagaa ggaacttcca
     4141 tattcattta ccaaaacagc gaaaaattgg aattgaaaga tccgagctca cttcaaccgg
     4201 gagatcgaac ggtaattccg agccaggcaa cgattatcag tcgctaccct aagttataaa
     4261 atccgacaaa agggaacaaa aagaatctta ataatgcttt ttgttccctt ttt
//
LOCUS       DAOSMM010000102         4226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_21026_length_4226_cov_14.298250, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000102 DAOSMM010000000
VERSION     DAOSMM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21026_length_4226_cov_14.298250"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2413)
                     /gene="leuS"
                     /locus_tag="PK898_09700"
     CDS             complement(<1..2413)
                     /gene="leuS"
                     /locus_tag="PK898_09700"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOU20343.1"
                     /translation="MEETIVPIYNPGKIEKKWQRIWADEKIYEANIDDSRPKHYALTM
                     LPYPSGNLHIGHWYAITPSDAHARYMRMQGYNVMFPMGFDAFGLPAENAAIKNNIHPK
                     KWTFQNIDHMREQLKSMGCMFDWRREIISAKPEYYRWTQWFFLQLYKHGLAYQKFSPV
                     DWCPNCNTTLAREQVWGEDRHCERCNTPVTKKDLNQWYFRITNYAEELLSFEGIDWPE
                     RVKTLQTNWIGRSEGASVLFKTEYGDDLEVFTTRPDTLWGATFMVIAPEHPLVDKLTK
                     PAQAESVKAYIEQAKKQSDIQRESVDREKTGVFTGSYAINPVNGARIPIWVADYVLMG
                     YGTGAIMAVPAHDQRDFEFARKFGLKVIAVVQPDDMAPLDGDTMKEAVPAHGRLINSG
                     EMTGVSGEEAFDRVVDWLEKNHLGHKTVTYRLRDWLISRQRYWGAPIPMIHCPKCGVV
                     PVPEDQLPVLLPDDVEWKPTGESPLKLHPTWKYTTCPVCGGKAERDTDTMDTFMCSSW
                     YHLGYLSPYYPTWPFDLKEYDYWMPVDVYTGGIEHATMHLIYTRFFHKACRDMGITKG
                     NEPMLKLRNQGSVLGEDNEKMSKSRGNVVDPDKLVATYGADTVRAYLMFFARWELGAP
                     WNSSGIEGSYRWLRRMWSMFLEPEEKNSEIDPEVLKKLRRKTYQTLRSVTGDFEEFQF
                     NTIVSSLMELLNEIAKAKQAGAWGTNEWNESVDVYLKMAAPVVPHITEEIWHLLGHSD
                     SIHLQKWPKMDEEAAKEEEITLVVQVNGKLRDRITLPSDYSEEEAKELVFKRQAVQAA
                     MGDKPV"
     gene            2643..3086
                     /locus_tag="PK898_09705"
     CDS             2643..3086
                     /locus_tag="PK898_09705"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOU20344.1"
                     /translation="MKKFRFVFIFIFILIFGTSIAVFAQDCNNCANPCAKDLGTYCVT
                     YHENNCLRAVPIDQNGYKYGDTVTVLFEPVLYKDGLIFYGWSHSPNGYANYGYAYNQF
                     IMPAQNVDLYAICIVPYYGPQQPPHPCHPFPPCNPPCGGGQQHPL"
     gene            complement(3181..3253)
                     /locus_tag="PK898_09710"
     tRNA            complement(3181..3253)
                     /locus_tag="PK898_09710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3218..3220),aa:Ala,seq:ggc)
     gene            complement(3295..3367)
                     /locus_tag="PK898_09715"
     tRNA            complement(3295..3367)
                     /locus_tag="PK898_09715"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Ala,seq:cgc)
     gene            complement(3565..>4226)
                     /locus_tag="PK898_09720"
     CDS             complement(3565..>4226)
                     /locus_tag="PK898_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU20345.1"
                     /translation="KAVSKWERGLSMPDVALLPPLANILEVTVTELLSYQKINETGKI
                     NMQEVGKLVSGTIHLSEKEQQKIKKHRQKRIYIYLSCICIVILELTFLRFHGYSRKDI
                     TDNILTFEILCFLFGSWICFFAKEKLPTYYDENKIHTYSDGIFRMNMIGINFNNKNWP
                     YILRSGRFILQLFAILIPVLYFLFMTFLPSIWNNAKVYILISPYIFWLIDMIIIGKHY
                     K"
BASE COUNT         1181 a          916 c          801 g         1328 t
ORIGIN      
        1 gtaccggttt atctcccatg gctgcttgaa ccgcttgcct tttgaatacg agctcttttg
       61 cttcttcttc agagtaatct gaaggtaaag ttatccgatc ccgtaatttg ccgttgacct
      121 gtacaacaag ggttatttcc tcttccttag cagcttcctc atccattttt ggccatttct
      181 gtaaatggat tgaatccgaa tgtccgagta agtgccagat ttcttcggtg atatggggga
      241 caaccggtgc ggccattttc aaatagacat ctaccgactc gttccattca tttgttcccc
      301 atgctccggc ttgtttcgct ttggcgattt cgtttaatag ttccattaaa cttgaaacaa
      361 tcgtattaaa ttggaattct tcaaaatctc cggttaccga tcgcaatgtt tgatatgttt
      421 ttctgcgaag tttcttcaaa acttccggat cgatttcgga atttttttct tccggttcaa
      481 ggaacatgga ccacattctt cgaagccagc gataagatcc ttcgattccg gaactgttcc
      541 atggcgcacc aagctcccag cgggcaaaaa acattaaata tgcccgaacc gtatctgcgc
      601 cataagtggc aacgagctta tccggatcaa ccacatttcc gcgacttttt gacatttttt
      661 cattgtcttc acctaaaaca ctcccttgat ttcggagctt gagcattggt tcattccctt
      721 tggtgatgcc catatcacga caggctttat ggaagaagcg cgtataaatc aggtgcatgg
      781 ttgcatgctc gattccgcct gtgtatacat caaccggcat ccaataatca tattctttca
      841 gatcaaacgg ccatgttgga taatacggac tgaggtaacc cagatgatac caactggagc
      901 acataaaggt atccatcgta tcggtatccc gttcggcttt cccgccgcaa acgggacagg
      961 tggtatattt ccatgtagga tgaagcttta atgggctttc tccggtgggt ttccactcga
     1021 catcgtcggg taataaaaca gggagttgat cttcgggtac cggaaccacg ccgcattttg
     1081 gacaatggat catcggaatg ggtgctcccc aataacgctg acgggaaatc agccaatcac
     1141 gcaaccgata agtaacggtc ttatgcccca agtgattttt ttcgagccaa tcgacgacgc
     1201 ggtcaaacgc ttcctcaccg gaaacgcctg tcatctctcc agaattgatt aatctgccat
     1261 gagcggggac ggcttctttc attgtatcac cgtccagcgg agccatatca tccggttgaa
     1321 cgacagcgat cacttttaaa ccaaattttc gcgcaaattc aaaatcacgt tgatcatgag
     1381 cgggaaccgc cataatcgcg cccgttccgt agcccattaa tacataatcg gcaacccaaa
     1441 tcggaattct ggctccgttt accggattta ttgcatatga tccggtaaaa acgccggttt
     1501 tttctctgtc aaccgattct cgctgtatat ccgactgctt ttttgcttgt tcgatatatg
     1561 ctttgacact ttcggcctga gccggtttgg ttaatttatc aaccaaggga tgctcgggtg
     1621 caataaccat aaaggtggct ccccacagcg tatccgggcg tgtggtgaaa acttcgaggt
     1681 catctccgta ttccgttttg aaaagaacag aagccccttc tgatcgaccg atccaattcg
     1741 tctgcagcgt ttttactctt tctggccagt caatcccttc aaaacttaac aattcttcgg
     1801 cgtaattcgt aattcgaaaa taccattgat tcaaatcttt ttttgtaacc ggcgtattac
     1861 aacgttcgca atggcgatcc tcgccccaaa cctgttcacg agccagtgtt gtgttacagt
     1921 tcgggcacca atccacggga gaaaatttct gataagctaa gccatgtttg tatagttgca
     1981 gaaaaaacca ttgcgtccaa cgataatatt cgggctttgc actgattatt tctctgcgcc
     2041 aatcgaacat acatcccatt gatttcagtt gttcacgcat atggtcgata ttttgaaaag
     2101 tccatttttt aggatgaatg ttattcttga ttgcggcatt ttccgcggga agaccgaaag
     2161 catcgaagcc catggggaac atgacattat atccttgcat tctcatataa cgggcgtgag
     2221 cgtccgatgg agtaatcgca taccaatgac cgatatgaag atttccggaa ggataaggaa
     2281 gcatcgttaa tgcataatgt ttcggtctcg aatcgtcaat atttgcttca taaatttttt
     2341 catcagccca tatccgctgc catttcttct caatttttcc gggattataa atcggaacaa
     2401 ttgtttcctc catttttatt cctccgctga aagttcattc taaactcaaa atattatagt
     2461 ctatggatga tgaatgtagt ttgtaaactt tttattccgt taccgataaa tgattgaatt
     2521 cttaaccgat tttatagctt gtttcaggaa atttccggaa taagaaggat ttgcttgaca
     2581 ttcatattaa atccttatat attataaata caactgtttc ttggatttaa aaaggagaaa
     2641 atatgaaaaa attccgattt gttttcattt ttatttttat tttgattttt gggacaagca
     2701 tagcagtttt tgctcaggat tgcaataatt gcgctaaccc atgtgctaag gatttgggaa
     2761 catactgtgt aacctatcat gaaaacaatt gtttacgtgc ggtaccgatc gaccagaatg
     2821 gatataaata cggagatacc gttaccgtct tatttgaacc tgttctttat aaagatgggt
     2881 taattttcta tggttggtct cattcaccaa acggatatgc caattatggg tatgcatata
     2941 accaatttat tatgccggct caaaacgtcg atctttatgc tatctgcatt gtgccttatt
     3001 acggtccgca gcaaccccct catccttgtc atccattccc cccatgcaat ccgccttgcg
     3061 gaggtggtca gcaacacccc ttataagatg atcggattta tggttttaaa aatagaggga
     3121 aaaacaaaaa ggagcattga aaccttcaat gctccttttt tctagcgcat tactctttag
     3181 tggacctagt gagattcgaa ctcacgacct tctcaatgcc attgagacgc gctcccaact
     3241 gcgctatagg cccgtttaat gattatacct tcctatttgg agcctcgcct aaagtggacc
     3301 tggggggatt cgaacccccg acctcttcag tgcgattgaa gcgcgctccc aactgcgcta
     3361 caggccccgg tttctgaagg ctgtgttatt ttataacgga gcgttatctg tgtcaaggtc
     3421 attcggtatt tagagtatcc ggtaattaaa ggtgaaacct gtaaaaaatt ctgcaaatga
     3481 tttgaaaaat aaatcggagc aggatttatt ttgacaatat atcaattgat aaatattaaa
     3541 taatattaat caagattaca ttatttattt gtaatgcttt ccgatgataa tcatgtcaat
     3601 taaccaaaaa atataaggag aaatcaaaat atacactttt gcattgttcc aaattgacgg
     3661 aagaaatgtc ataaataaaa aataaagaac cggtattaag atagcaaaca gctgaagaat
     3721 aaaacgcccg ctacgtaaaa tgtaaggcca atttttatta ttaaaattaa ttcctatcat
     3781 attcatgcga aatattccgt cgctatatgt atgaatttta ttttcatcgt aataagtcgg
     3841 caatttttcc tttgcaaaaa aacaaatcca actgccaaat aaaaaacaaa gaatttcaaa
     3901 agttaaaata ttatctgtta tatcttttct tgaatatcca tgaaagcgaa gaaaagtaag
     3961 ttccaatata acaatacaaa tacatgacag atagatataa attcgctttt ggcgatgttt
     4021 ttttattttt tgttgttctt tttcagaaag atgaatagtc ccactgacta atttcccaac
     4081 ttcctgcatg tttatttttc ctgtttcatt tattttctgg tagctgagca attcggtaac
     4141 tgttacttcc aaaatatttg ccagaggagg aagtaatgcg acatcaggca tactcaatcc
     4201 tcgttcccat ttactgactg ctttat
//
LOCUS       DAOSMM010000103         4207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_21160_length_4207_cov_7.088632, whole genome shotgun sequence.
ACCESSION   DAOSMM010000103 DAOSMM010000000
VERSION     DAOSMM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21160_length_4207_cov_7.088632"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PK898_09725"
     CDS             complement(<1..690)
                     /locus_tag="PK898_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU20346.1"
                     /translation="MNLIKSNINVDDPQPSAAKPKQLKVHNRKIVMSAFQDATPHSII
                     DISQQTGVSRQTVAKALDHFLKLGIVEICGKGDSTDNGGKKPNIYQLSSVPKFLVVHL
                     HEYYIELFDLNCNLIGRQDLNPLDDSPHDFDYYLNAVRRMADDLFEQHEIDENDLYGV
                     VAITPGPVIDHAFVHRNPFCSEWGFDIPVKEKLEAAFPTAKCVFADNIGKLAGLGSIT
                     ANLEFCSSKRVV"
     gene            857..1906
                     /locus_tag="PK898_09730"
     CDS             857..1906
                     /locus_tag="PK898_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU20347.1"
                     /translation="MKKSLLLLLVVVMLFSAVSAISAADKPLAGVIWYNFADTFIQNA
                     RQSLLNVAKADGTIEITDADSNNDVATQTNNINNMFAQGVKYLVLNNINTNAISEICE
                     QAKAEGVTTIFANTDSPSDEDFANYENLYLVTSRATQSGTIMGEAVAKYWKEHPEADR
                     NKNGKLDYIMLLGFTWHYDTRVRAENSIKAVEDAGIETSRIGGELIAEYNRGKAQEMV
                     AALLANYSDDIDAVFACNDDMALGAIEALKAGGFFKDEKTYIPVCGVDATAVGVDALK
                     DGTLLVTSLNNPVKLGKAVYKTMWLLENGKEVTTETLGMDGVEVEGHRIWINYTPITK
                     DNIEDAKYDITDPSF"
     gene            1981..3528
                     /locus_tag="PK898_09735"
     CDS             1981..3528
                     /locus_tag="PK898_09735"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU20348.1"
                     /translation="MKDSEYVLEMKNISKSFPGVKALDQVDFYVEPGSVHALMGENGA
                     GKSTLMKCAFGMYHPDEGEIFINGLPVQLTNPREALDHGIAMIHQELQSIRTRSAMEN
                     VWVGRIPYRKAFGVHWVDDKKLYQDTKALMDDLGINVDPYAKAGDLSASQCQMLEIAR
                     AVSYNARIIIMDEPTSSLTEAETNLLFHIIRKLTAKGVAIIYISHKIDEILEIAQNVT
                     IMRDGKIVGTWPAADLNTDLIVKRMVGREMSNRFPEKKHRPAEEVCLEIRHFTSAGSR
                     GFRDINLSVRRSEIFGIGGLVGAQRTELVESIFGLRAISEGEILINGKSVIVKSPEHA
                     IQNSIALLTEDRRKSGIFPRFSVLENIVIAKQSADTSVYKKGIFLDNQKRAADAARFA
                     EAFSIKTPSLKTEIRFLSGGNQQKVVLARWMLIDPNILILDEPTRGIDVGAKYEIYSL
                     MEKMASEGKTIIMISSEMPELLGMSDRIAVMCEGHLSGILEGERATEENVMYLASSYK
                     NAEKVLN"
     gene            3528..>4207
                     /locus_tag="PK898_09740"
     CDS             3528..>4207
                     /locus_tag="PK898_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20349.1"
                     /translation="MEKQKKFSIQHFFMNYALYIIMLLMIVIIGMMSPKFFSFRVIRD
                     ILMQSSTRIIVAMGCMYILVSGAADLSGGRTVGLAAVVAGSLAQKSDYYIKFWPNLPE
                     MPLIVPVIAAVLIGLIVGLMNGFTTAKLKVPSFLATLAVMMIAYGANQLYCNKPPNNS
                     QPLGGFLKSFSNLGTGSFLSVPYIIYIAIGVCIFQHLLLTRTCLGREIFACGSNQEAA
                     RVSGINVY"
BASE COUNT         1191 a          814 c         1033 g         1169 t
ORIGIN      
        1 cacgacgcgt ttggaggaac aaaattcgag attggcggtt atggaaccca atccggccag
       61 tttcccgatg ttatcggcaa aaacgcactt tgccgtcgga aaagccgctt caagcttttc
      121 tttgacggga atgtcaaaac cccattcaga acagaacgga tttcggtgga cgaaggcatg
      181 atcgataacc ggacccggtg taatcgcgac caccccgtat aggtcatttt catcgatttc
      241 atgttgttcg aataggtcat ccgccattct gcggacggca ttcagataat aatcaaaatc
      301 atgcggcgag tcatctaaag ggttaagatc ctgacggccg atcagattac agttcagatc
      361 aaacagttcg atgtaatatt catgcagatg gactaccagg aacttcggga cggatgaaag
      421 ctgataaatg ttgggttttt tccctccgtt atcggtcgaa tcacctttcc cgcatatttc
      481 gacaatcccc agtttaagaa aatgatccaa tgcttttgcg actgtctgcc ggctgacacc
      541 tgtttgttga gaaatgtcaa tgatcgagtg aggcgtcgcg tcctgaaagg cgctcattac
      601 aattttgcgg ttatggacct ttaattgttt cggctttgca gctgacggct gcgggtcatc
      661 cacgttgata ttcgatttaa ttaagttcat ttctttccga gcgccctgtc gacttcaagc
      721 gctaacaata tgactttaat caatttagga ttggagtaac atcactaatc caagtttaca
      781 aaataattat aatgcaatta cgttacgcaa tgttatttcg taactaattg atataacgta
      841 gataggaagg aaagatatga aaaagtcatt gttgctgtta ctggttgttg taatgctgtt
      901 ttctgctgtc agcgctatca gcgctgcgga taaaccctta gccggggtta tctggtataa
      961 ctttgccgat acatttatac agaatgcccg acaatccttg ctgaatgttg ccaaagcgga
     1021 cggcacaatc gaaataaccg atgccgattc gaataatgat gttgcaactc aaaccaacaa
     1081 tatcaataat atgttcgctc agggtgtaaa atacctcgtt ttgaacaata ttaataccaa
     1141 tgccatcagc gaaatctgcg agcaggctaa agccgaaggt gtaacgacga tttttgcgaa
     1201 taccgattcg ccgtctgatg aggattttgc caattatgag aatctttact tggttacttc
     1261 tcgtgcgact cagtcgggga caattatggg agaagcagta gccaagtatt ggaaagaaca
     1321 tccggaagct gatcggaaca agaacggaaa gttggattac attatgctgt tggggttcac
     1381 ctggcattat gatacacgcg ttcgggcaga aaattccatc aaagcggtgg aagacgcggg
     1441 tatcgagacc agccgaatcg gcggtgagtt gattgccgaa tacaaccggg gaaaagctca
     1501 ggaaatggtt gcggcattgt tagcaaacta cagcgatgat atcgatgcgg tttttgcgtg
     1561 taacgatgat atggcgctgg gtgctatcga agctctgaaa gccggcggtt tcttcaaaga
     1621 tgaaaaaacg tatatcccgg tttgcggtgt tgacgcaaca gcggtgggtg ttgatgcttt
     1681 gaaagacggc acgctgctgg ttacctctct gaataacccg gtgaaactgg gcaaagcggt
     1741 ctataagacg atgtggctgc ttgaaaacgg taaagaagtg acaaccgaga cattgggcat
     1801 ggatggtgtt gaagttgaag gtcatcgtat ctggatcaat tacactccga ttaccaagga
     1861 taatatcgaa gacgcaaagt atgatattac ggatcccagc ttctaatcat ggaagctgtc
     1921 taaagtactt gaagcagccg gttattccgg ctgcttcata atatcgagca ggagaagcaa
     1981 atgaaagatt ccgaatacgt tttggaaatg aaaaacatca gcaaatcatt cccgggtgtc
     2041 aaagcgctgg atcaggttga tttttatgtc gaaccgggga gtgttcatgc attgatgggc
     2101 gagaacggag ccggaaaatc gaccttaatg aaatgtgctt tcggcatgta tcatcctgat
     2161 gaaggagaaa tatttatcaa cggactgccg gtacagttaa cgaatccgag agaagcattg
     2221 gatcacggga tcgccatgat tcatcaagaa ctgcaatcca tcaggacaag atcggcgatg
     2281 gaaaatgtct gggtagggcg gatcccttat cgtaaagctt tcggtgttca ttgggtcgat
     2341 gataaaaaat tgtatcagga taccaaagcc ttaatggatg atctgggaat caatgttgat
     2401 ccgtatgcca aagccggtga tttgtcagct tcacaatgcc aaatgctcga aatagccaga
     2461 gcggtttcat ataacgcccg aatcattatt atggatgaac cgacttcttc tttaacagaa
     2521 gccgaaacga atctcttatt tcatattatt cgaaaactga cggcaaaagg tgtcgcaatt
     2581 atctatattt ctcataaaat cgatgagatt ctggagatcg cacaaaatgt aacgattatg
     2641 cgggacggga agattgtcgg aacatggccg gctgctgatt tgaataccga tttgatcgtg
     2701 aaacgaatgg tcggtcgtga aatgagcaat cggtttccgg agaagaagca tcgaccggca
     2761 gaagaggtat gtttggaaat ccgccatttt acaagtgccg gttcacgcgg tttccgcgat
     2821 attaacctga gcgttcgtcg cagtgaaata ttcggtatcg gaggattggt cggagcacaa
     2881 cgaactgagt tagtcgaatc gattttcgga ttaagggcaa tttccgaggg agaaatttta
     2941 atcaacggga agtccgtaat cgttaaatca ccggaacatg ctattcaaaa cagcatcgcc
     3001 cttttgaccg aggatcgtcg gaagagcgga atttttccgc gattttcagt actggaaaat
     3061 attgttatcg ccaaacaatc tgccgataca tctgtttata agaaggggat ttttcttgat
     3121 aaccaaaaac gtgctgcgga tgcggcgaga tttgcggaag cattttctat taaaacccct
     3181 tctcttaaga ctgaaattcg ttttttgtcc ggaggaaatc agcaaaaagt cgttttggca
     3241 cgatggatgt taattgatcc aaatattctt attcttgatg aacccacgcg cggaattgat
     3301 gtcggtgcaa aatatgagat ttattctttg atggagaaga tggcaagcga agggaaaacg
     3361 atcatcatga tcagttcgga gatgccggaa ctcttgggga tgtcggatcg gattgccgtg
     3421 atgtgcgaag gacatttatc gggaattttg gaaggagaaa gagctacgga agaaaatgtg
     3481 atgtatttag ccagctctta taagaacgcg gaaaaggtac taaactgatg gaaaaacaga
     3541 aaaaattcag cattcaacat ttttttatga attatgcctt atatatcatt atgttattaa
     3601 tgattgtcat tattggcatg atgtccccga aatttttttc attcagagtt attcgtgata
     3661 ttttaatgca aagttcaact cgtattatcg ttgcgatggg ttgtatgtat attcttgttt
     3721 cgggggcggc cgacctttcc ggcggtcgaa cggtaggttt agctgctgtt gttgcgggat
     3781 cattggcaca gaagtccgat tattatatta aattctggcc gaaccttccg gagatgcctt
     3841 tgatcgttcc ggtaatcgcc gcagttttga ttggcttgat tgtcgggctt atgaacggtt
     3901 ttactacagc aaaattgaag gttccttcct ttttggcaac attggcggtg atgatgatcg
     3961 catacggtgc gaatcagcta tattgcaaca aaccaccgaa caattcccaa ccgttgggag
     4021 gattcctcaa atctttctca aatcttggga cggggagttt tttgagtgta ccttatatca
     4081 tctatatagc gatcggtgtc tgtatcttcc aacacttatt gctgacaagg acttgtttgg
     4141 ggcgggaaat atttgcctgc ggaagcaatc aggaagcggc gcgtgtttcc ggaattaatg
     4201 tctataa
//
LOCUS       DAOSMM010000104         4100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_21899_length_4100_cov_15.252658, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000104 DAOSMM010000000
VERSION     DAOSMM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21899_length_4100_cov_15.252658"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(336..659)
                     /locus_tag="PK898_09745"
     CDS             complement(336..659)
                     /locus_tag="PK898_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOU20350.1"
                     /translation="MEKQTAGRTALGTFAPKFAELNDDVLFGEVWSREDQLSLRDRSI
                     ITVTVLMTKGIFDESMKHHLMNAKKNGVTAEEMAEVITHAAFYAGWPNAWAVFRLAKE
                     VYSEE"
     gene            complement(696..1478)
                     /locus_tag="PK898_09750"
     CDS             complement(696..1478)
                     /locus_tag="PK898_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOU20351.1"
                     /translation="MALLQINFYSETLHRKVPVQVILPTDVSPGNTEPYKTLYLLHGL
                     TENCMAWTSNTRIERWATERNLAVVMPSGENSFYVDHPFGSSCFGDFGAYIGQELVEM
                     TRKMFRLSTDRADTFIAGLSMGGFGALRNGLKYHETFGYIACLSGAIHIFEYPFDDPD
                     RNIIGEDACFGDIRAAALTDKNPRVAAEILFEKKRTDPNVSFPKVYIACGTEDYLLKA
                     NRQFAQFLRINGVSVTYEEGPGKHDWDFWESYIQRVLEWLPI"
     gene            complement(1618..2757)
                     /locus_tag="PK898_09755"
     CDS             complement(1618..2757)
                     /locus_tag="PK898_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU20352.1"
                     /translation="MAIQPVKTAIVGCGVISEAYLETMTRKFKILNVVGCCDLNQRKA
                     EIRAQKYGIRVLSMEEILADPSIELVVNLTTPTAHYGVIKQLLEGGKHVYTEKVLSVE
                     LEQAAELVRIADQKGLYLGAAPDTFLGSAIQTARYVVDSGMIGEISSCACTLTRDSEL
                     FSRAFPFTAQPGGGIGFDVGIYPITALLSILGPVAEVSGIIQTRKPERYQLSPEHFGE
                     PFRIECENLMSGMLRFHCGTIGTLLFDANSIFTQPEKPNLILFGTLGILYMADPNQFG
                     GDVKVILKGNNEPFVMQQSHAFSGECRGLGVAEMAWSLRRGRKNRANKEMAFHALEVL
                     HGIVRSSETRTSIVMQSTFEKMPPIPRGYSGQNFFDFIEEAGIAL"
     gene            complement(2783..3670)
                     /locus_tag="PK898_09760"
     CDS             complement(2783..3670)
                     /locus_tag="PK898_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20353.1"
                     /translation="MDISRLRKQIAERDSYSFLETVDVFSGKRTQLKEFDFVIEAPNW
                     TKDGRFLVYNSMGKIFRFELSTGTIEEIDTGFATDCNNDHVLSPDNAQLGISHFTNED
                     AISRIYILPFTGGNPRLVTENGPSYLHGWSPDGKRLAYCAERNGQFDIYTISVAGGEE
                     TQLTNQPALDDGPEYSPDGRSIWFNSARTGLMQLWRMNTDGSDQTQMLHEEANCWFPH
                     ISADGKWVVYIAYRKGDVAPGDHPANKDVELRLIPSEGGESKTIARLFGGQGTINVNS
                     WAPDHHTVAFVSYKLKGSD"
BASE COUNT         1083 a         1026 c          820 g         1171 t
ORIGIN      
        1 tcggaagatt tagcgaaaag acttgttatt cgatctattt tagagacctt ttccttgata
       61 aagcgaaacg gtaaacacag gagaaagcca ttatttaacg aatctttata cattcggtat
      121 ttatcggtga aataatttgt aaaatgccaa tttatcttct tgatatcatg attattttca
      181 tataatcggt gtctaaatta tctatatacc aacccatatt caataagcta tcatattatt
      241 gtctttttat aaaaatgatc cgctccatag ctgaaatatc ccgcccctgt acggcatacg
      301 cactcgctta cgccttaagg ggcggctcag agttcctatt cttcgctata tacttccttt
      361 gccagccgaa acaccgccca tgcattcggc caacccgcat aaaaagcggc atgagtgatc
      421 acttccgcca tctcctcagc cgtaaccccg ttcttcttgg cattcataag atgatgcttc
      481 atcgattcat cgaagatccc cttcgtcatc aaaaccgtaa ccgtaataat gcttcgatcg
      541 cgcaacgaaa gctggtcttc tcttgaccat acttctccga acagcacatc atcgttcagt
      601 tcggcaaatt ttggagcaaa ggtaccaaga gcggttcttc ctgcagtttg tttttccatt
      661 ttaattccct atttcctttc atcttttgaa tgattttaga tcggaagcca ttcaagcacc
      721 cgttggatat aggattccca aaaatcccag tcgtgctttc ccggtccttc ctcataagtc
      781 acagagactc cgtttatccg taaaaattga gcgaattgcc gatttgcctt caataaataa
      841 tcctctgtac cgcaggcgat atatactttc gggaaagaaa cgttcggatc cgttcgcttt
      901 ttttcgaata agatttccgc agccactctc ggatttttat cggtcagcgc cgcggctcga
      961 atgtcaccga aacaggcatc ttctccgatg atattacggt cgggatcatc aaacggatat
     1021 tcgaaaatat gaatggcgcc ggaaaggcaa gcgatatatc caaaagtttc atggtatttc
     1081 aaaccattcc gcagcgcacc gaatccgccc atcgaaagcc cggcaataaa cgtatctgcc
     1141 cgatcggttg aaagacgaaa cattttccgt gtcatttcga ccaattcctg cccgatataa
     1201 gcgccgaaat ccccgaaaca ggaactgccg aacggatgat ccacataaaa cgaattttcc
     1261 ccggacggca tcactacggc caaattccgc tcggttgccc aacgctcaat ccgagtattg
     1321 gatgtccaag ccatacagtt ttccgtcagc ccatgaagta aatataatgt cttatacggt
     1381 tctgtattcc ccggtgacac gtctgtggga aggattacct gcaccggaac ttttcgatgt
     1441 agtgtctcgg agtaaaaatt tatttgaagc aaagccatcg cgtcttttct cctttctttc
     1501 ggtctgatcg atcggccgtc ccatcaaggg gtgatttgcc gatcatttct ataggacacg
     1561 cttcccctta tatgcaatcg gctctttgaa aatccctgca gcacgatagg atgatcgcta
     1621 aagcgctatt cccgcctctt cgataaaatc aaaaaagttt tgacccgaat agcctcgcgg
     1681 gataggcggc atcttttcga aggtggattg catcacaatg ctcgttcggg tctcactgct
     1741 gcgaacaata ccgtgcaata cttccagagc atgaaatgcc atttctttgt tggcccgatt
     1801 ttttcttccc cgtcgcaatg accaagccat ttcggctacg ccgagtccgc ggcattcgcc
     1861 ggaaaaagca tgactttgtt gcatcacgaa aggttcgtta tttcctttta agatcacctt
     1921 gacatcaccg ccaaactgat tgggatctgc catatacaga attcccagcg ttccgaacaa
     1981 aatcaaattg ggcttctccg gttgagtaaa aatcgaattc gcatcgaaca aaagtgtccc
     2041 gatcgttccg caatgaaatc gcagcatacc gctcattaga ttttcacatt cgatgcggaa
     2101 aggctcaccg aaatgttccg gtgacagctg atatcgctcc ggcttgcgag tctgaataat
     2161 tccggacacc tcggcaaccg gacccaaaat gctcaataag gcagtgatgg gatagatgcc
     2221 cacatcaaaa ccgattcctc cgcccggttg agcagtgaaa ggaaaggcgc ggctgaacag
     2281 ttcactgtct cgcgtcaaag tgcatgcaca ggaagaaatt tcgccgatca tgccactgtc
     2341 cacaacgtac cgtgctgttt ggatcgctga acccagaaag gtgtcgggag ccgctcccag
     2401 atataaccct ttttgatcgg ctatcctaac caactcagcg gcttgttcca attccacaga
     2461 caaaaccttc tccgtataaa catgtttgcc gccttccaac aactgtttaa tgacaccgta
     2521 atgcgctgtg ggcgtggtca ggttgacaac aagttcaatg cttgggtcag ccaggatttc
     2581 ttccatgctg agtacccgga tgccgtattt ttgtgctctt atctcagcct tccgttggtt
     2641 caaatcacag catccgacga cattgagaat cttaaacttt cgcgtcatgg tttccaaata
     2701 agcttcgctg atcaccccgc atccgacgat agcggtcttt accggttgaa ttgccattct
     2761 atttgcctct ttcctcagaa gattaatcgc ttcccttaag tttgtatgaa acaaaagcta
     2821 cggtatgatg atccggtgcc caagagttca cattgatcgt cccttgtccg ccgaacaaac
     2881 gggcgatcgt tttcgattcg cccccttcgg acgggatcag tcgtaattcc acgtctttgt
     2941 ttgccggatg atcgccgggc gcaacatcgc cttttcgata agcgatataa actacccatt
     3001 tcccatccgc cgaaatatgc ggaaaccaac aatttgcttc ttcatgcaac atttgagttt
     3061 gatcggatcc gtccgtattc atccgccaga gctgcatcag tcctgtacgt gctgagttaa
     3121 accaaatcga tctcccgtcc ggagaatatt ccggaccgtc atccaaggcg ggttggttgg
     3181 tcaattgcgt ctcctctccg cccgcgacag aaatcgtgta tatatcaaat tgtccattgc
     3241 gttcggcgca ataggcaagc cgttttccgt ccggtgacca gccatgcaaa tagctgggac
     3301 cgttttcggt taccaaacgc ggattcccgc ccgtgaatgg caaaatatat attcgagaaa
     3361 tggcatcttc gtttgtaaaa tggctgattc ccaattgagc attatcggga gataaaacat
     3421 ggtcgttgtt acaatcggtg gcaaaaccgg tgtcaatctc ttcgatcgta ccggttgata
     3481 actcaaatcg aaagattttg cccatactgt tatagaccaa aaatcgaccg tcttttgtcc
     3541 aattcggtgc ctcgataaca aaatcaaatt ctttcagttg ggtccttttt ccgctaaaga
     3601 catcaacggt ctctaaaaag ctgtaggaat cccgctctgc gatttgtttg cgtaaacgac
     3661 tgatatccat attgtttgta ctccgatttt cataatggtt gtgcgaaatt atcttcattg
     3721 gaatggagat agaaacgccg gggctgttga tcgctgattc atcgaaaaat cccattcaaa
     3781 atacttacgg ttttatttta tttatgccat attccgaata aaaagcgatt caaccagtgc
     3841 aacgaatata ctgatttttt atctattaat aaattgtaac cccaacttac cgatattttt
     3901 agaatcggga aaagtaaaat atttcctctg tcttttgttt gattcggata tcaatccgaa
     3961 attcttaaat gataatctgt tatttgaaaa tagctatacg aaatatattg ggacagagac
     4021 gatttcagct gttatccggt aaaccaaact cttctttaat agttaacgta acttccccgt
     4081 tccgctgcgg aagttttttg
//
LOCUS       DAOSMM010000105         4083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_22011_length_4083_cov_17.545680, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000105 DAOSMM010000000
VERSION     DAOSMM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22011_length_4083_cov_17.545680"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1002
                     /locus_tag="PK898_09765"
     CDS             166..1002
                     /locus_tag="PK898_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOU20354.1"
                     /translation="MEKIRVALQGVQGAHTTIAVDKAFPGKNVELVVFPTYELAFEAV
                     ENKDCDYAVIPMENSMQGSIHSNYYLLARKFLYIVNEVYVPIHYSLIGLPDAKIEDIS
                     LVIAHPGALEVCRGYIESMPRKPNIETVYDSAASVEMLLNFADPETAMVGSKSLADFH
                     QLAILDEDIEDDLNNVTRYNVFAREPVNPGPGGKTTLVFTTRHQPGALLNALRVFAER
                     KIDLTKIESRPLPDQPYEYMFYADISGSISDPNVKEAIDELEQYHAPWIRVLGSYMSI
                     KD"
     gene            complement(1105..2127)
                     /locus_tag="PK898_09770"
     CDS             complement(1105..2127)
                     /locus_tag="PK898_09770"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOU20355.1"
                     /translation="MQHEVTVIQDLLDSNGHLREPGWAKKPVFNYRRKDIKASRFRIK
                     EWDYYLILNDHHAIALTLADNSYMGMISASVLDFDSGTEHTGSIMTAFPMGKFKMPET
                     ADESHCHFENKRAKVDFLATEGTRTIFFEYKNFWEGKDLHAEFTLTQPFEDRIVIATP
                     WKEDPKAFYYNQKINCMPAKGFYTLGDHRVDFHESDTFGTLDWGRGVWTRDNTWYWGS
                     GNGWVEGGRFGFNFGYGFGDTSAATENALLYEGMLTKVDEVKFLPSDPDHLLMPWRIE
                     SNDDRVDLDFSPILDRAAAINALILSTDQHQVFGRVSGNVLLENNRRLDIRDLTCFFE
                     IVHNVY"
     gene            complement(2239..2730)
                     /locus_tag="PK898_09775"
     CDS             complement(2239..2730)
                     /locus_tag="PK898_09775"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOU20356.1"
                     /translation="MKACCFYESPVGMLGIAENGTAITGLFFGNTMPSGEWQLRNTPL
                     LAQAVDQLEAYFSGERTAFDLPLDAEGTVFQRSVWEALRRIPYGETRSYGQIAEAIGK
                     PKAGRAVGMANHRNPIAIIIPCHRVIGSDGSLVGYGGGLNVKTFLLRLEQGISERDKN
                     NLR"
     gene            complement(2836..3375)
                     /locus_tag="PK898_09780"
     CDS             complement(2836..3375)
                     /locus_tag="PK898_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOU20357.1"
                     /translation="MELKGSQTEKNLEAAFAGESKATNKYSYYASQAKKDGFEQIGGI
                     FSETSGNEREHAKLWFKYLHGGEIPSTPANLLDAAAGEHYEWTEMYKGFAETARQEGF
                     SEIAARFELVAKVEKGHEERYRKLAANIDNKKVFEKDEEVVWICRNCGYLHYGKKAPE
                     KCPTCGHPQSFFEVHCENY"
     gene            complement(3403..3792)
                     /locus_tag="PK898_09785"
     CDS             complement(3403..3792)
                     /locus_tag="PK898_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOU20358.1"
                     /translation="MSLIRFEVYQCETCGNQVIKSIDGGGELVCCEKPMKKLEVNSSD
                     GAAEKHVPVYEYLGSREMLVRVGSLDHPMTEEHYIGWIAVVYQNTVIWEKLKPGMAPQ
                     IIANVESGYNGEIYAWCNLHGLWMAHF"
     gene            complement(3797..3961)
                     /locus_tag="PK898_09790"
     CDS             complement(3797..3961)
                     /locus_tag="PK898_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOU20359.1"
                     /translation="MKKYICPGCQYIYDPAVGDPDGGIPAGTAFEDLPDDWVCPVCGL
                     AKDEFEEYEE"
BASE COUNT         1060 a         1086 c          830 g         1107 t
ORIGIN      
        1 gggtcggttt ggaaccgacc cctacgatat tttgttcaaa tcgggacgtc ggggacaccg
       61 ttccttatat catgtataca ccttgataaa ccactccctg ggatatatac gaacgaccgt
      121 ttcaccgtta taattaatag catcaagacg gaggagagag aaatcatgga aaagatccga
      181 gttgcactcc agggtgtgca aggcgcccac acgacaatag cagttgataa agctttcccc
      241 ggaaaaaatg ttgaactggt tgttttcccg acttatgaat tggctttcga agccgtcgaa
      301 aataaggatt gtgactacgc ggtcattccc atggaaaatt ccatgcaagg cagcattcat
      361 tccaattatt acctccttgc ccgaaaattc ctctatattg tcaatgaggt ctatgttccc
      421 atccattatt ctctgatcgg acttcccgat gcaaaaatcg aggatatctc gctggtcatc
      481 gctcatccgg gcgcattgga ggtttgccgg ggatacatcg aatcgatgcc gcggaaaccg
      541 aatatcgaaa cggtctacga ttcggctgcc agcgttgaaa tgctgctgaa tttcgccgat
      601 ccggaaacag ccatggtcgg ctccaaatcc ctggccgatt ttcatcagct tgccatcttg
      661 gatgaagaca tcgaagatga cctgaataac gttacccgat ataatgtttt cgcgcgcgaa
      721 ccggtcaatc cgggacccgg cggaaagaca acgttggtat tcaccacccg ccatcagccc
      781 ggtgcactgc tcaacgcatt gcgcgttttt gcggaacgca agatcgacct gaccaaaatc
      841 gaatccagac cgctgcccga tcaaccttac gaatatatgt tttatgccga tatctcgggc
      901 tcaatttccg atccgaatgt caaagaggcc atcgatgaac tcgaacaata tcatgcgccg
      961 tggattcggg ttctcggcag ttatatgtcc ataaaagatt aggcaacttt tacgcaatca
     1021 aacggaacag tcataaaacc cgggcggtct ttcgaacccg ggttttattt tcgtcatctc
     1081 caaaagaacg ccgaacagat tcgatcaata gacattatgt acgatctcga aaaagcaagt
     1141 cagatcgcgt atatccaaac ggcggttatt ctccaacaga acatttccgg agacacgtcc
     1201 gaatacctga tgttgatccg tggacaaaat cagagcattg atcgcagctg cccgatccaa
     1261 tatcggggaa aaatccagat ccacccgatc atcattggat tcgatccgcc aaggcattag
     1321 aagatgatcc ggatcggacg gcaaaaattt gacttcatcc accttggtca acatcccttc
     1381 atacaacaga gcgttttccg tcgcggcgct ggtatcgccg aacccgtaac cgaaattgaa
     1441 tccgaaccgg ccgccttcca cccaaccgtt tccggagccc caataccagg tattatcgcg
     1501 cgtccaaaca ccgcgtcccc aatcaagtgt gccgaaagtg tccgattcgt gaaaatcaac
     1561 ccggtggtct ccgagtgtat aaaaaccctt cgccggcata caattgatct tctgattata
     1621 ataaaaagcc ttcggatcct ctttccaagg agttgcgatg acaatccgat cttcaaacgg
     1681 ctgagttaat gtgaactcgg catgcaaatc ttttccttcc caaaaatttt tatattcgaa
     1741 aaagatcgtt cgtgtccctt cggtggcgag aaaatccact ttcgcccgct tattttcaaa
     1801 atgacagtgg ctttcgtcag ccgtttccgg catcttaaat tttcccatcg ggaaagcggt
     1861 cattatcgaa ccggtatgct ccgtcccgga atcaaaatca agtaccgaag ccgaaatcat
     1921 ccccatatag ctgttgtcgg ccaaagtcag cgcaatcgca tgatgatcat ttaagatcag
     1981 ataataatcc cactccttaa ttcgaaagcg acttgccttg atgtcttttc gccgataatt
     2041 aaaaaccggt tttttcgccc accccggttc ccgcagatga ccgtttgaat ctaacaaatc
     2101 ctgaatgacc gttacttcat gttgcatagg aaacctcctg atcggtatcg atttttgaaa
     2161 gattgcagtc gattgaattt tccaaaatcg atccggtaaa agaagcgatt aaagaacccc
     2221 tgaggcgatt ttctttcttt acctcaaatt gtttttgtcc ctttcgctga ttccttgttc
     2281 caatcgcaat aaaaacgttt tcacgttgag tcccccgcca tatcccacca aggatccgtc
     2341 ggaaccgatc acgcgatgac agggaataat gattgcaatc ggatttcgat gattcgccat
     2401 ccccactgct ctgccggctt tcggttttcc gatcgcttcg gcgatctgac cgtaggatcg
     2461 ggtctccccg taagggatcc tccgcagcgc ttcccagacc gaacgttgaa ataccgttcc
     2521 ctccgcatcc agcggaagat caaaagctgt tcgttcaccg gaaaaataag cctcgagctg
     2581 atcgacggct tgcgccaaca acggcgtatt ccggagctgc cattcgccgg aaggcattgt
     2641 attcccaaag aacaggccgg taatcgctgt cccattctcc gcgatcccca acatgccgac
     2701 cggtgattca taaaaacaac aggctttcat tatcagcccc ttttcattcc gtgatgatta
     2761 tccctatttc aaatatacca aaaaaactgc cttccggcag tttttttgat ttccatttca
     2821 aagaatcggc gaaaattaat aattctcgca atgaacttca aagaaagact gcggatgacc
     2881 gcaggtgggg catttttccg gagctttctt tccgtaatgc agatatccgc aattccggca
     2941 aatccaaacc acttcttcat ctttctcgaa aactttctta ttgtctatat ttgccgccag
     3001 ctttcgatat cgttcctcgt ggcctttttc tacctttgct accagctcaa aacgggctgc
     3061 gatttccgag aacccttcct gtcgggcagt ttcggcgaaa cctttgtaca tttccgtcca
     3121 ttcataatgc tcaccggcag ccgcatccaa caaatttgcc ggagtgctcg gaatttctcc
     3181 gccgtgcaga tatttaaacc ataatttggc atgttcgcgt tcgtttccgg aggtttccga
     3241 aaaaatgccg ccaatctgtt caaatccgtc ttttttcgcc tgtgaagcgt aataagaata
     3301 tttattcgtc gccttggatt caccggcaaa tgccgcttca aggttctttt ctgtttgtga
     3361 tccttttaat tccataatga tcctttcttt cggatatgtc ggctaaaaat gagccatcca
     3421 taaaccatgc aggttacacc aagcataaat ttcgccgttg taacctgatt ccacattggc
     3481 tataatttgc ggggccattc cgggtttcag cttttcccag atgacggtat tttgatagac
     3541 cacagcgatc catccgatat agtgctcttc cgtcatcgga tgatcgaggc ttcccacccg
     3601 caccagcatt tcccgactgc ccaaatattc gtagaccgga acatgctttt ctgcggcgcc
     3661 atcgctcgaa tttacctcga gctttttcat cggtttttcg caacaaacca gttcgccacc
     3721 gccgtcaatg cttttaataa cctgatttcc gcaggtctca cattggtaaa cttcgaatcg
     3781 aatcaatgac atcggattac tcttcgtact cctcaaattc atctttcgct aacccgcaaa
     3841 ccgggcaaac ccaatcatcc ggaagatctt caaaagctgt tccggcggga atcccgccgt
     3901 cagggtcacc cactgcgggg tcataaatat actggcatcc cgggcatata tactttttca
     3961 tgttgtttct ccttctatga atatgattct tggtatggcg aaacaaaaca aatctactgt
     4021 ttcaattgaa aattttaaca tagatttatt ttttcgggac tcgatgtcaa atgggacatt
     4081 ccc
//
LOCUS       DAOSMM010000106         4070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_22117_length_4070_cov_20.385554, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000106 DAOSMM010000000
VERSION     DAOSMM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22117_length_4070_cov_20.385554"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PK898_09795"
     CDS             <1..1347
                     /locus_tag="PK898_09795"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HOU20360.1"
                     /translation="QSFAGIESDVIHQVLPSYVITYCIVLIALGVFVNYKKSGQPFSQ
                     YVTMGIQNLSQIAIISGALAAGIAAVIAIFIYLILDNSYSDLIVRAEILVLGCTVGIG
                     TLHSMIHTHKETAKFFAVVVRYILLSLTMIAFAIIYLYIAKIIITQEMPSNEVFRILA
                     ALFVVGLPIWTMADSFPKDNFLVRTGIKLPYIFIPFLFLQGYSIGIRIAEFGLTPRRY
                     LCVMLMLFEILYILFYFFKKREVGVILPILAVLAIITTAVPGINVYDLSVRSQKNNFE
                     KYAAIGFKNLSESEQKKMAGAYYYLKNDPFGKKYVENIDTEMMEAIQNSGFYGVNSEG
                     QNYNYRFYSVNDLDISGYGRMTVVSANLSGDSIDLTNVPVGNDTEPHILEADVSQAVK
                     QILTEKTSEEDLKRSEPAPIIEIGDGSILVLSDIAFSTTEEGTVGSLNLQGFWLRP"
     gene            1522..>4070
                     /gene="gyrA"
                     /locus_tag="PK898_09800"
     CDS             1522..>4070
                     /gene="gyrA"
                     /locus_tag="PK898_09800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOU20361.1"
                     /translation="MGTDEVNIVDIDEQMRMAYLDYAMSVIVSRALPDARDGLKPVHR
                     RILYAMQDMGIRSNGPHKKSARIVGEVLGKYHPHGDQSIYDAMARMSQDFSMRYPLVD
                     GQGNFGSIDGDSPAAMRYTEARIAPITDELLADIDKDTVDFTDNFDGTLREPLVLPSK
                     IPNLLLNGSSGIAVGMATNIPPHNLREISDAIKYLIENYDDVDNITVEELMRHVQGPD
                     FPTGGVIIGREGITQAYSTGRGRIVVRGRTHVEENRPGRYDIVITEIPYQINKTTLIE
                     RIAELVRSGKIDAVHDMRDESDRSGMRIVLELKKNGQPKQVLNLLYKHSPLQSTFGVQ
                     MLALVDNQPRTLSLKKALCIFIEHRLVIIQRRSQYELRKAKEREHILDGLLIALANLD
                     EVIALIRSSKDADTAKSGLIERFHLTEIQAQAILDMQLRRLSALERQKIEDEKREISV
                     KIASLEELLASPKLQLDVVEKETDEIAKKYGDDRRTTIEVDAIENLSESDLVEDRSVF
                     VTLTDHGYIKRVNADVYRTYNRGALGVAGHGLKEEDELSKLVFANTLDTMLFFTNKGR
                     VYSEKVYRIMECGRTDKGTPVVNIINLEGEERVTELIAVSDFSPDAYLVMVTANGKIK
                     RNALSDYANVRTNGIIAISLDDDDELKWVLRTDGKCDVILATEKGKGLRFPEKSVRSM
                     GRQARGVRGMRIAADDRLACVDVAKEDDYLLVVGEKGIGKRTPVRNIPRHGRATGGVM
                     ITNKRTLDVTGKVISAIVLHENDDVTFITASGHVVRLKGDSIPVNGRTARGVRLIRLA
                     EGDTVAAVTSNSPDDIPVETLPESTDLEPETEDIENADEETDEDETEEITE"
BASE COUNT         1077 a          866 c         1082 g         1045 t
ORIGIN      
        1 cagtcgttcg ccggcataga gtcagacgtt atccatcagg tattgccttc ttatgtgata
       61 acttattgca tcgttctgat cgctttgggt gttttcgtga attataaaaa atccggccaa
      121 cctttcagtc aatatgtcac gatgggaatt caaaatctgt cccaaatcgc tatcatcagc
      181 ggtgccctgg cggccggtat cgctgcggtt attgcgatat tcatttatct gattttggat
      241 aattcctatt ccgatctgat tgtacgggcg gagatattgg tattggggtg cacggtcggc
      301 atcgggactc tgcattctat gattcacacc cataaagaaa ccgcgaaatt ttttgcggtc
      361 gttgtccggt atattctgct gtccttaaca atgatcgcat ttgcaatcat ttatttatat
      421 atagcgaaaa tcattattac tcaggaaatg ccttcaaacg aagtatttcg gatccttgcc
      481 gcactttttg ttgtagggtt acccatctgg acgatggcgg attcttttcc aaaggacaat
      541 tttctcgttc gaacggggat caagcttcct tatattttta ttccctttct ttttctgcaa
      601 gggtattcca tcggaatccg gattgcggaa ttcggattga cacccaggcg atatctctgt
      661 gtcatgctga tgctgtttga aattctctat atccttttct attttttcaa gaaacgggaa
      721 gtcggagtta ttttaccgat tctggcggtg ttggcaatca taacgacggc ggttccgggg
      781 atcaatgtgt acgatttatc ggttcgcagc caaaaaaaca actttgaaaa atatgcggcg
      841 atcggtttca aaaatctgtc cgaatcggaa cagaaaaaaa tggcgggagc ttattattat
      901 ttgaaaaatg atcctttcgg aaaaaagtat gttgaaaata ttgatacgga gatgatggaa
      961 gccatccaaa acagcggctt ttacggggtc aattccgaag gacaaaatta caattaccga
     1021 ttttattcgg taaatgattt ggatatttcg ggttatggca gaatgacggt tgtatccgcg
     1081 aacctgtccg gggattcgat cgatttgacg aatgttccgg tggggaatga taccgaaccg
     1141 catatactcg aagcggatgt atcgcaggcg gtaaaacaaa ttctgacgga aaaaacctcg
     1201 gaagaggacc tgaagcgcag cgaacctgcc ccgatcatcg aaatcggtga cggcagtatc
     1261 ttggtgctgt ccgacatcgc tttttcaacg accgaggaag ggaccgtcgg gtcattgaat
     1321 ctgcaagggt tctggctgcg accctgaatc gagttcgaat aactaaatat cgatttgatc
     1381 gacggacgat cggtcttgat ttcagccggc ggcaacccgc cggctttgtt ttccgtcacc
     1441 gccgcagccg ctccggggtg aaggggcggt cttcggatgg tataattaat cggatatttt
     1501 acgagaaaag agcgaataaa aatgggaacg gatgaagtaa atatagtcga tatcgatgaa
     1561 cagatgcgca tggcttatct cgattatgcg atgagtgtaa ttgtgtcgcg cgcattgccg
     1621 gatgcccggg atggcttgaa gccggtccat cgaagaattc tatacgcaat gcaggatatg
     1681 ggcattcgtt ccaacggtcc gcataaaaaa tcggctcgta ttgtgggcga ggttctggga
     1741 aagtatcatc cccacggtga tcaatccatt tatgatgcga tggctcgtat gtcgcaggat
     1801 ttctcgatgc gctatccatt ggtggacggg cagggaaatt tcggatccat tgacggcgat
     1861 tcaccggcag ccatgcgtta taccgaagcc agaatcgcac cgatcaccga cgaattgttg
     1921 gcggatatcg ataaagatac cgtcgatttt accgataact tcgatgggac attacgggaa
     1981 ccgctcgttt taccttcaaa aattcccaat ttgttgctga acggttcttc cggcatcgcg
     2041 gtcgggatgg cgacgaatat cccgccgcat aatttacggg aaatatccga tgcaatcaaa
     2101 tatttgatcg agaattacga tgatgtggat aacatcaccg tcgaagaatt gatgcgccac
     2161 gttcagggac cggattttcc gaccggcggg gtcatcatcg gtcgcgaggg tatcacccaa
     2221 gcttattcga ccggtcgcgg aagaattgtt gtccgcggac ggacgcatgt ggaagagaac
     2281 cgtccgggaa gatatgacat tgttattacg gaaatccctt accaaattaa taaaacgacc
     2341 ttaatcgaac gaatcgcgga actggtacga agcggaaaaa tcgacgccgt tcatgacatg
     2401 cgagacgaat cggatcgcag cgggatgcgt attgtgttgg aactgaaaaa gaacggacag
     2461 cccaaacagg ttctgaatct gctctataag cattcaccgt tgcaatccac attcggtgtt
     2521 cagatgctgg cgttggtgga taatcaaccg cgtaccctgt ccttaaaaaa ggcgctttgt
     2581 atttttatcg agcatcgtct ggtgattatt caaagacgtt cccagtatga attgcgcaaa
     2641 gcgaaggaac gcgagcatat tttagacggt ttgttgatcg ctttagccaa tttggatgag
     2701 gtgattgcgc tgattcggag ttcgaaagat gccgacaccg ccaaatcggg tttgatcgaa
     2761 aggtttcatc ttacggaaat tcaggcacag gcgattttgg atatgcaact gcgtcgcctc
     2821 tccgcattgg aacgccaaaa aatcgaagac gaaaaaagag agatttcggt gaaaatcgct
     2881 tccctggagg agctgttagc cagcccgaaa ctccaattag acgttgtcga aaaagagacg
     2941 gacgagatcg ccaaaaaata cggtgatgat cgccggacaa cgattgaagt cgatgcgatc
     3001 gagaatcttt cggagagcga cttggtggaa gaccgctccg tctttgttac gctgaccgat
     3061 cacggctata tcaaacgtgt aaatgcggat gtttatcgca cctataatcg gggtgcgttg
     3121 ggtgtggcgg ggcacggact gaaagaagag gacgagttat caaagctcgt ttttgcgaac
     3181 acattggata cgatgttgtt cttcaccaat aagggccggg tctattccga gaaggtttat
     3241 cgaattatgg aatgcggccg caccgataaa ggtacgcccg ttgtcaatat tatcaatttg
     3301 gaaggcgagg aaagggttac ggaattaatc gcggtctccg atttttctcc ggacgcttat
     3361 cttgtcatgg tgacagccaa cgggaagatc aaacgaaatg cgctctccga ttacgccaat
     3421 gtccgcacca acggcattat tgcaatcagc ctggacgatg acgatgaatt gaaatgggta
     3481 ctgcggaccg atgggaaatg cgatgtgatt ttggcaaccg aaaaagggaa agggttacgt
     3541 tttccggaaa aaagtgtccg ttcgatgggc aggcaagcca ggggtgtgcg gggtatgcgt
     3601 atcgccgcgg atgatcgttt ggcatgtgtc gatgtggcaa aagaggacga ttatttattg
     3661 gtggtcggag aaaaagggat cggcaaacgt acgccggtcc gaaacatccc tcgccacggt
     3721 cgggcgaccg gcggagtgat gatcaccaat aaacgtacat tggatgtgac cggtaaggtg
     3781 atttccgcga tcgttctgca tgagaatgac gacgttactt tcatcaccgc ttcggggcat
     3841 gtcgtccgtt tgaaaggcga ttcgattccg gtaaacggtc ggaccgcacg cggagttcgt
     3901 ctgattcggt tggccgaagg ggataccgtt gccgcggtta ccagcaattc tccggatgat
     3961 ataccggtcg aaacgcttcc cgagagcacc gatttggagc cggaaacgga agatatcgaa
     4021 aacgctgatg aagaaacaga cgaagatgaa acggaagaaa ttacggagga
//
LOCUS       DAOSMM010000107         4061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_22183_length_4061_cov_19.863205, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000107 DAOSMM010000000
VERSION     DAOSMM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22183_length_4061_cov_19.863205"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PK898_09805"
     CDS             <1..732
                     /locus_tag="PK898_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOU20362.1"
                     /translation="DLYQGPLDLLLDLIEKAELDITVLSLAQVTDQFLEYIKNMEGDN
                     PAEVSAFVVIAARLIQIKSSALLPHSETDIRELDEDSGEALAQQLILYRRFKQLAGWL
                     AQREDLNYHSYERMGTPDIGIEAPLDLSNLTVIHLAAIAGRLFAKKPEQPELATMISQ
                     PRITIGQRIQLLIGKVREKRKISFRKLLSSGSKVEAVVTFLAMLELIKRNVISVNQDD
                     LFADIEIRASDESDQEQSLVSEFGE"
     gene            complement(791..1798)
                     /locus_tag="PK898_09810"
     CDS             complement(791..1798)
                     /locus_tag="PK898_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU20363.1"
                     /translation="MESIIYHDEKVIDVYLSIPFSSYLTLFGASMLPNFKIGVGTIAW
                     GNQLIWDYGIAYDEQSLYEVFSRSLQNGIRFFSTSEAFTDGDSERILGRFAAKHPEQA
                     FISTKYVPRPWRIRRSDFLRAMNASLARLQLRRLDLYQIGQPTGLMGIPMLVECAAEA
                     LDTGMVKNIGLSGFKANEIEQFSDLLNRLGGAVSCVETEYNLLNREIETNGVLKICQD
                     LHIPIIAQCPLAMGLLTGKYLNEPIVNGLRRKIMQRYPANGLNGLIRLMNLIGSEHEG
                     RNSAQVALNWLIQKKVIPIPGIKNVEQLDQNRTVTEWDLKPEEVTRLDDYSAGLAVGT
                     E"
     gene            1894..3402
                     /gene="glpK"
                     /locus_tag="PK898_09815"
     CDS             1894..3402
                     /gene="glpK"
                     /locus_tag="PK898_09815"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOU20364.1"
                     /translation="MTKYIAAIDQGTTSSRCILFNQAGEIVSSDQMEHDQILPKPGWV
                     EHNPIQILRNVETIFENAVRKADAEPGDIAAIGITNQRETTVVWNRYTGIPYQNAIVW
                     QDTRTAALCDVLSRRHGGQDRFREKTGLPISVYFSGIKLRWLFDQVDGLYEEALRGNL
                     MFGTIDSWLIWNMTGGITGGKHYTDVTNASRTMLMNLKTLSWDSEILEELDIPVQILP
                     EIKPSSGYFGAYHKDGAVIPISGVFGDQQAALFGQTCFAAGEGKNTYGTGCFLLLNTG
                     SVPVESKNGLITTVGFKIEDRPPVYCLEGSVANAGSLVQWMRDNLKMINSSAEIEALA
                     REVPDNGDVYIVPAFSGLFAPYWRSDARGIIAGLTRFSNRGHLARAVLEATAFQTMEV
                     VEAMKKDSGVDLSMLKVDGGMTVNELLMQFQADILEVPIVRPVIRETTALGAAYGAGL
                     EIGYWHSLDELKSKWAADREWKPQIDPEKKAKLCARWKKAVTKSMGWIDPES"
     gene            complement(3426..>4061)
                     /locus_tag="PK898_09820"
     CDS             complement(3426..>4061)
                     /locus_tag="PK898_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOU20365.1"
                     /translation="AMIASIVVAIGISVLSDQEEIKEDTAIGIFFTAALALGIALIST
                     IRTYAVDLTHVLFGNVLGVTTDDIRFILITGAIVLLAVTVFYRFFQVVTFDPVMAKTL
                     RWNVKLIRTGLLVLIACTITISINTVGSGLVTAMLITPAATALMFTKKLYTTMLAAAG
                     IGALSGFLGLYLSYFLNISSGAAIVLVATLFFIIVYFYRKLFPYRNKKIEA"
BASE COUNT         1090 a          977 c          992 g         1002 t
ORIGIN      
        1 gatctctatc agggaccgct tgatttgctg ttggatttga ttgaaaaagc ggagctggat
       61 attacggttc tttccttggc acaggtaaca gatcagtttc tggaatatat caagaacatg
      121 gagggggata atcccgccga agtttccgct tttgtggtga tcgccgcccg cttaatccaa
      181 attaaatcat ccgcacttct gcctcattcg gaaacggaca ttcgggaatt ggatgaagat
      241 tccggcgaag cattggcgca acaattgatt ttgtaccgcc ggtttaaaca gttggcaggt
      301 tggttggcac aacgggaaga tttaaattat cacagttatg agcggatggg tacaccggat
      361 atcggtatcg aagcgccgtt ggatttgagt aacctgacgg ttattcatct tgccgctatc
      421 gccggcaggt tattcgccaa aaaaccggaa caacccgagc tggcgacaat gatcagccag
      481 ccgcgcataa caatcgggca acgcatccaa ctgctgattg gcaaagttcg cgagaaaagg
      541 aagatttctt tccgaaaact cttatcatcc ggatcgaaag tggaggcagt ggtcaccttt
      601 ctggcgatgc tcgaactgat caaacgaaat gttatttcgg ttaatcagga tgatcttttt
      661 gccgatatcg aaattcgcgc ctcggatgaa agcgatcagg agcaatcttt ggtttccgaa
      721 ttcggagaat aattttttct tttccccgcc gactggaatc atacagatac gatttttagg
      781 agtgatgcgt tcactccgtc ccgacagcca aaccggctga ataatcatcc agtctggtga
      841 cttcttccgg cttcaagtcc cattcggtaa ccgttcggtt ttgatccaac tgctcaacat
      901 ttttgatacc cgggatcggg ataacctttt tttgaatcag ccaattcaac gctacctgtg
      961 cgctgttacg accttcgtgc tcggatccga tcaggttcat cagacggatt aatccgttta
     1021 atccgttcgc cggataacgc tgcataattt tccgtctcag accattaacg atcggttcgt
     1081 tgagatattt cccggtcagc aaacccatcg ccaatggaca ttgcgcgata atcggaatat
     1141 gcaaatcctg acaaatctta agcactccgt tggtctcgat ctcgcgattc aaaaggttat
     1201 attccgtttc aacacaagag accgcacctc ccaatcgatt cagcagatca ctgaattgtt
     1261 caatttcatt cgctttgaaa ccggagagcc cgatattttt caccataccg gtatcaagcg
     1321 cttccgccgc gcattcaacc agcatcggaa tgcccatcaa tccggtcggc tgaccgattt
     1381 gataaaggtc aagccggcgc agctgcaacc gagcaagcga agcattcatc gcccgtaaaa
     1441 aatcactgcg gcgaattctc catggacgcg gcacatactt ggtggatata aaagcttgtt
     1501 cgggatgttt tgccgcaaat ctgccgagaa tccgttcgct gtctccgtcc gtaaatgctt
     1561 cactggtgct gaaaaaacga attccgtttt gaagagagcg gctgaacact tcgtacagcg
     1621 actgctcgtc ataagcgatc ccgtaatccc aaatcagttg gtttccccag gcaattgttc
     1681 cgacacctat tttaaaattt ggcagcatgg acgctccgaa caaagtcaga taacttgaaa
     1741 acgggattga taaatataca tcaatcactt tttcgtcatg ataaatgata ctttccaacc
     1801 attataaaag gtttccgctc agaaccgtaa aacatctctt tataattgga atagaatctc
     1861 aaggtttttg aggcgggggg aaaaggggca tccatgacga aatatattgc ggcaatcgac
     1921 caaggcacta ccagcagtcg ttgtattctg tttaatcaag ccggagagat cgtttcgagc
     1981 gaccaaatgg aacatgacca aattctgcca aaacccggtt gggtggaaca caatccgatt
     2041 caaattctcc gaaacgtgga aactattttc gaaaatgccg ttcgcaaagc cgatgcggag
     2101 ccgggagata tcgccgcgat cggcattacc aaccaacgtg aaacgaccgt ggtttggaat
     2161 cgatataccg gtatccctta tcagaacgcc atcgtttggc aggatacccg caccgccgct
     2221 ctctgtgatg tgttatctcg caggcatggc ggtcaggatc ggtttcgtga gaaaacagga
     2281 ttgccgattt cggtctattt ttcggggatt aagttacgct ggctttttga tcaggttgac
     2341 ggtctttatg aggaagcttt gcgcggaaat ttaatgttcg ggacaatcga ttcctggtta
     2401 atctggaata tgaccggcgg aataaccggc ggcaaacatt acaccgatgt caccaacgct
     2461 tctcggacga tgttgatgaa cctgaaaacg ctttcttggg atagcgaaat cctggaagag
     2521 ttggacattc cggttcaaat tctaccggag atcaaaccgt cctccggtta tttcggcgca
     2581 tatcataaag acggcgccgt catccccatc agcggtgttt tcggcgatca gcaggcagcc
     2641 ttgttcggtc aaacctgttt cgccgccggc gaagggaaaa atacgtatgg aaccggctgt
     2701 tttctgctgc tcaacaccgg atccgttccg gtggaaagca aaaacggatt gatcactacc
     2761 gtcggtttta aaatcgaaga ccgccctccg gtttattgtc tggaaggttc ggttgccaat
     2821 gccggatcct tggtgcagtg gatgcgggat aatctgaaga tgatcaactc gtccgcagaa
     2881 atcgaagcct tggcacggga agttccggac aacggcgatg tttatatcgt tcccgctttt
     2941 tccggtttgt tcgccccgta ctggcgcagc gacgcacgcg gaatcatcgc cggactgact
     3001 cgattttcca accgcggaca tttggcccga gcggttctgg aagctacggc tttccaaacc
     3061 atggaggttg tggaagcgat gaaaaaagat tccggagtgg atttgagtat gctgaaagtc
     3121 gatggcggaa tgaccgtcaa cgaattactg atgcaatttc aggcggatat tctggaagta
     3181 ccgatcgtcc gaccggtcat tcgcgagaca acggctttgg gcgcggctta cggagcggga
     3241 ttggaaatcg gttattggca ttcgttggat gaactgaaaa gcaaatgggc tgcggatcga
     3301 gaatggaaac cgcaaatcga ccccgaaaag aaagccaaac tgtgcgctcg ctggaaaaaa
     3361 gccgtcacta aatccatggg ttggatcgat ccggaatcat gaagtaacca atcaaccgtt
     3421 cgggatcagg cttcgatctt cttattgcga taagggaaca gttttcgata aaaataaacg
     3481 atgatgaaaa agagggtggc aaccaagaca atcgcggcgc cggaactgat attgagaaaa
     3541 taactcagat aaagccccaa aaatccggat aacgcaccga tccccgccgc cgccagcatg
     3601 gtggtataaa gtttcttggt aaacatcaac gccgttgccg ccggagtaat cagcatggcg
     3661 gtgaccaatc ccgagccgac cgtgttgatg gaaatcgtaa tggtgcatgc gatcaaaaca
     3721 agcaatccgg ttcgaatcaa tttgacattc cagcgcagcg tctttgccat gaccggatcg
     3781 aatgtgacaa cctgaaaaaa acggtagaaa acggttacgg caagcagaac gattgcgccg
     3841 gtaatcagga taaaacggat atcatcggtt gtgactccca acacatttcc aaacaaaaca
     3901 tgcgtcagat ccaccgcata ggttcggatg gtactgatca aagcgattcc cagtgccagc
     3961 gccgcggtaa agaaaatccc gatcgcggta tcctctttga tttcttcctg atccgacaat
     4021 actgaaattc cgattgcgac cacaatcgaa gcgatcatgg c
//
LOCUS       DAOSMM010000108         4029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_22438_length_4029_cov_18.454957, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000108 DAOSMM010000000
VERSION     DAOSMM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22438_length_4029_cov_18.454957"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="PK898_09825"
     CDS             <1..1629
                     /locus_tag="PK898_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20366.1"
                     /translation="YLLMSVLFFSFLLTVRILHHSSHRILFTAVALILSAINLIASEY
                     FFMLEFLRPVFIWIDLSKQNLSGKERRKQTFRNELPYFCVLLLGIIYRLFFNKTQTLH
                     YEFSLIDELKTSPLSAVGDYFVSIANDLFKVLFAAWGRIFDFPEKQVLGEKTFIGYCL
                     VCLLGFLLTALFLFLITRHHPTPNQKVAIQLIITGLIALILAGQPFWLTKSYLSFVFP
                     NSRYTLPFLLGMSFFWTGILLLFPLHRPAVRISICLLSAVMIGLSSGSHFMVANEYRR
                     DWTLTKDFFHQLKWRIPAIEENTVVITNQLPIRFSTDNSLTAPLNWIYAQDYEKDSMP
                     YMLYTNVKREETLSKFEKGKKIYQEYLSAKFEGNTEDAVSIYYNAPGCVHVLDPEVDV
                     LNQTIPVIDRDAALLNNYSRILTDVPYEPLDPVLFGEEPSKNWCWYYEHADLARQQKN
                     WELVTKLGDEALLLDDHPNDPMERLPFIEGYANSSQWDQAYRQTEEAMKVTPVMNNPL
                     CALWNRIQNETIHSDEKEVTIQNISRLLDCSFLN"
     gene            1639..2769
                     /locus_tag="PK898_09830"
     CDS             1639..2769
                     /locus_tag="PK898_09830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU20367.1"
                     /translation="MNSTDRNPKILIICMTRRGGLLQFSDCLVKNLAQLCPVAVVTAK
                     NAEHSEADWDQNTIAKFVLDMGEGKKGTFKKLFSLDTWKTLSRALNEFKPDIVHITAA
                     QEWNPFIGFYIRFIRRKPLVYTIHDVTYHEGIPAYFQITESLFRKIPNDLIVLTQKGK
                     EQLIQKGKKADRILVVPHGVYDFFTLYSKAEIEPQPQILFFGRIEPYKGLEILLKAAE
                     KVLKEHPDWKLHVAGGGDLTPYQQYLSNPQLIVTNRFLSNEEVALAMQQASIVALPYL
                     SASQSGVIPTAFAFRKAVIATNVGGIPDMVKNMETGILIPPNNVPALEKALNLLIESP
                     ELRRRLGENGYRFAHEELGWGAIAKKHVAFYRNILDRKKKEK"
     gene            2776..3618
                     /locus_tag="PK898_09835"
     CDS             2776..3618
                     /locus_tag="PK898_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU20368.1"
                     /translation="MEKIPDWLKIWSELAEIRNTHFALQEEGQSDHWSENARKFSDRV
                     RERWQEPDTSRDFLKSVLFKNPESTLLDIGAGTGNWAIYLSPYAKRITALEPSAGMRA
                     VLEENLMLSEPKVDNVTVIAGKWPETDVPEHDFSLCSHAMYGIPNFEEAVRKMNAITR
                     KNCFLLIRAPLLNDPLAQIAQKVWGQPNDSPNFTVAYNALIQMGIYPDVLFEENRHIH
                     PKVYPSYESAFSDIKSRFGLENDSQFDTFIRSVLKDHLVQSENQWILQHNVKVGLISW
                     KSIR"
     gene            complement(3709..>4029)
                     /locus_tag="PK898_09840"
     CDS             complement(3709..>4029)
                     /locus_tag="PK898_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20369.1"
                     /translation="ILVVNYFSDPKVSVEQLKKSTGWDQIRAVKENQIFPFAKDTFSW
                     DSSSPRWGLGLLWTFKTLYPESAEDIDIDAEMLKFYRWFGLSDESIQANLIDLFHAEP
                     QLTK"
BASE COUNT         1155 a          899 c          838 g         1137 t
ORIGIN      
        1 taccttttaa tgagcgtcct gttcttttca tttctgctca ctgtccggat tttgcatcat
       61 tcaagccacc gaattttgtt caccgctgtc gccctcatcc tgagtgccat taatctaatc
      121 gcctccgagt actttttcat gcttgagttt ctgcgtccgg tattcatttg gattgattta
      181 tccaagcaaa atctctccgg aaaagagcgc cgtaaacaaa cattccgcaa tgaacttcct
      241 tacttttgcg tccttcttct cggcatcatc tatcgattgt ttttcaacaa aacccaaact
      301 ttgcattatg aattctcttt aatcgatgaa ttaaaaacaa gtccgttgag tgctgttggc
      361 gactattttg tttcaatcgc gaacgattta ttcaaagttt tgtttgccgc ctgggggcgc
      421 attttcgatt ttcccgaaaa acaagtactt ggggaaaaaa cttttatagg ctactgtctg
      481 gtttgtttac tcggattctt attaaccgct cttttccttt ttttgatcac aaggcaccat
      541 ccgactccga atcagaaagt tgcgattcaa ttaataataa cgggactgat tgcgttgatc
      601 ttggcaggac agcctttctg gttgacaaaa tcttatcttt cctttgtttt tccgaacagc
      661 cgttacactt tgcctttttt gctcggaatg tctttcttct ggaccggtat tctgctttta
      721 ttcccattgc accggccggc ggtcagaatt tcaatatgct tgctctcagc ggtcatgatc
      781 ggtctaagca gcggatccca tttcatggtt gccaacgagt atcggcggga ttggacactg
      841 acaaaagatt tctttcatca attgaaatgg cgaattcccg ccattgaaga aaatacggta
      901 gtcattacaa accagcttcc catccgcttt agtacagata attctttgac agctccctta
      961 aattggatct atgcgcagga ttatgaaaaa gactcgatgc cgtatatgct ctacacaaat
     1021 gtaaaacgag aggaaacact cagcaaattc gaaaaaggaa aaaagattta tcaagaatac
     1081 ctttccgcca aattcgaagg aaacaccgaa gatgccgtca gcatctatta caatgctccc
     1141 ggctgcgtcc atgttctcga cccggaagtg gatgtcctta accaaacaat tcccgtgatc
     1201 gatcgggatg cggctctttt gaataattac agccgaattc tgacggatgt gccttatgag
     1261 ccgcttgatc cggtattatt tggcgaagaa ccgtctaaaa attggtgctg gtattatgaa
     1321 cacgccgatc ttgcccgtca acaaaaaaat tgggagctgg tcacaaaatt aggggatgaa
     1381 gctttgttgt tggatgatca tccgaacgat ccgatggaac gtcttccttt tatcgaaggt
     1441 tacgctaaca gctcacaatg ggatcaagcc taccggcaga cggaagaagc gatgaaagtg
     1501 acacccgtaa tgaacaatcc gctctgtgct ttgtggaatc gaattcaaaa tgagacgatt
     1561 cattccgacg agaaggaagt tacaattcaa aatatcagcc ggctgttaga ttgcagcttt
     1621 ctcaactgac gaggatagat gaactctacc gatcgaaatc cgaaaatcct gataatttgt
     1681 atgacccgcc ggggcggatt gctccaattt agcgactgtt tggtcaaaaa tctggcacaa
     1741 ctttgtcccg ttgccgttgt tacggcaaag aatgcggaac acagcgaagc ggattgggat
     1801 caaaatacga ttgccaaatt cgttttggat atgggtgaag ggaaaaaagg tacttttaag
     1861 aaattattca gtctcgacac atggaaaact ttatccagag cgttgaacga attcaaaccg
     1921 gatatcgtgc atattacggc tgcacaggaa tggaatccct ttatcggatt ttatattcga
     1981 tttattcgga gaaaaccgct tgtttacacc attcatgacg taacctatca cgaagggatt
     2041 cccgcctatt tccaaattac cgaatcattg tttcgcaaaa tcccgaacga tctcattgta
     2101 ttaacccaaa aaggaaaaga acagctgatt caaaaaggga aaaaagcgga ccggatcttg
     2161 gttgttccgc acggtgtcta tgattttttc acgctttaca gcaaagctga aatcgaaccc
     2221 caaccgcaga ttctattttt cggccggatt gaaccgtata aaggattgga aatcttactg
     2281 aaagcagccg aaaaggtttt gaaagagcat ccggattgga aacttcatgt tgccgggggc
     2341 ggagacctga ccccctacca acaatatctt tcaaatccgc aattgattgt cacaaatcgt
     2401 tttctcagta atgaagaagt agccctcgcg atgcaacagg cttccattgt tgctttaccc
     2461 tatctttccg caagccaatc gggagtcatt cctaccgctt ttgcctttag aaaggctgtt
     2521 atcgcaacaa atgtcggtgg tattcccgac atggtaaaaa atatggaaac ggggatattg
     2581 ataccgccca ataatgttcc cgcgcttgag aaagctttga atctgttgat agagtcccct
     2641 gaattacgaa ggcgtttagg agaaaacggt tatcgctttg ctcatgaaga actgggatgg
     2701 ggagcaatcg caaaaaagca cgtcgctttc taccgaaata ttttagatcg gaaaaagaag
     2761 gagaaataaa ggattatgga gaaaataccg gactggctga aaatatggag cgaattggcg
     2821 gaaatccgaa acactcattt tgccttacaa gaggaaggac aatcggatca ttggagcgaa
     2881 aatgccagaa aattctccga ccgtgtgaga gaacggtggc aagagcccga tacctcacgt
     2941 gatttcctaa aatcggtttt attcaaaaac ccggaatcaa ctttgctgga tatcggcgcc
     3001 ggcacaggca actgggcgat ttatctgtct ccctatgcga aaagaatcac cgccttagag
     3061 ccctctgcgg ggatgagagc tgtccttgaa gaaaatctga tgctgtccga accaaaagtt
     3121 gataacgtca cagtcatagc gggtaaatgg ccggaaaccg atgtgccgga acatgacttt
     3181 tcactatgtt cccatgctat gtacggaatc ccgaatttcg aggaagctgt tcggaagatg
     3241 aacgccataa cccgaaagaa ctgtttttta ctgattcgcg cgcctttgct caatgatcca
     3301 ttagcccaaa tcgcccaaaa agtttgggga cagcccaatg acagccccaa tttcactgtc
     3361 gcatataatg ccttaattca gatgggaatt tatcccgatg tcttattcga agaaaatcga
     3421 cacattcatc caaaagtcta tccttcctac gaatcggctt ttagtgatat taaaagccga
     3481 ttcggccttg aaaacgacag ccaatttgat acctttatcc gttccgttct taaagatcat
     3541 cttgttcaga gtgaaaatca atggatcctt cagcataatg taaaagttgg tttgatatct
     3601 tggaaaagta tccgttaact tacggaggga atcggtattc attcgatcat ttcaataaaa
     3661 aaggatgcct tttcgacatc ctttttgagt attaatcttt tcttcgctct atttcgtcag
     3721 ttgcggctcg gcgtgaaata gatcaatcaa attcgcctga atgctttcat cactcagtcc
     3781 aaaccatctg taaaacttaa gcatttccgc atcgatatca atatcttccg cagattccgg
     3841 gtaaagcgtt ttaaatgtcc agagcagtcc caatccccaa cgcggagaag atgaatccca
     3901 actaaaggtg tcttttgcaa acgggaaaat ctgattttct tttaccgcac gaatttgatc
     3961 ccaccctgtg gattttttca attgttcaac acttaccttc ggatcggaaa aatagttcac
     4021 cactaaaat
//
LOCUS       DAOSMM010000109         3853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_23737_length_3853_cov_15.451553, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000109 DAOSMM010000000
VERSION     DAOSMM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23737_length_3853_cov_15.451553"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK898_09845"
     CDS             <1..285
                     /locus_tag="PK898_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20370.1"
                     /translation="LGENMNYRNGIPDNQRFTVYILPTAEDIPPIQNVSVELYEATGP
                     YGAKGAAEVATVVIAPAITSAIESIRDLKINRLPVDFEQILDAKEKGSVR"
     gene            285..1127
                     /locus_tag="PK898_09850"
     CDS             285..1127
                     /locus_tag="PK898_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOU20371.1"
                     /translation="MDVIRPASLQDCLRILKTEHTIPVAGGTDLMVNLALNKISPDRI
                     VDISGLQELKEISLSDNFLKIGALVTFSRLAGREIAQYPFCNALREAAEKMGSPQIRN
                     RATIGGNLVTGSSAGDGSVAILALDAQVELTGLDGIRKVPLSLFYSGYHQTAIHSDEL
                     LTAVYIPIRPGTSSFQKVGKRNALAIAVASLAIYSYIRDSHFEQIYIAIGSVAPTPIR
                     AVKTDLFLQGQKITGGVIKEAARIIQDEIAPITDIRGTADYRRKLVANLLTDQLQQLS
                     ESKN"
     gene            1129..1596
                     /locus_tag="PK898_09855"
     CDS             1129..1596
                     /locus_tag="PK898_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU20372.1"
                     /translation="MSKVMLSCKINGEEIHSEIPSDMRLIDFLREEMNLTGVKEGCSE
                     GECGACTVLFNGDPVNSCILLAIQAEGAEITTIEGIAKDGKLSELQQAFVDHGAVQCG
                     FCTPGMILAAQALLDKNPHPTEAEIRRAISGNLCRCTGYQAIVDAILTVSQRK"
     gene            1810..>2510
                     /locus_tag="PK898_09860"
     CDS             1810..>2510
                     /locus_tag="PK898_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU20373.1"
                     /translation="MGDRIVTLCTAQWADLEFEELCKTASSMGYDGIEIATWGNSFDL
                     DKAATDDDYIQFIKSTLAKYNLQCKALAMHIIGQCVGDLPDPRLNNFAPARLANKPDE
                     IRAWAIESMKKGPKVASKLGVKIITCFTGSPIWRYLYSFPQTPESMVEEGFNEIVRLW
                     TPILDEFKKYDVKFALEVHPGEIAFDYYSTAKILEKFKDNDTFGINFDPSHLLWQGIQ
                     PHLFLRDFIKRVYHV"
     assembly_gap    2511..2610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2685..3842
                     /locus_tag="PK898_09865"
     CDS             2685..3842
                     /locus_tag="PK898_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU20374.1"
                     /translation="MEKKTITYGMVGGSNQAFIGDIHRKAIALDGRAKLVAGCFSSNP
                     DRNRETGETNHISPDRLYSDYQAMAKAESTRPDKIDFVSITTPNHTHYAIAKEFLLAG
                     INVVCEKPLCFTVEQAEELKKISEEKGLIFAVTYAYTGYTMVKVAREMIANGKIGKII
                     NVNAEYAQDWLLDEISENEKSSGKKFSFWRMDPEKSGISNCTGDIGTHMENMVHYLTG
                     LKIKRLHATINRFGHALDLNANIIVEYENHINGGYWCSQLAAGHKNGFVVRVFGTEGG
                     IEWAEEFPDYLKFTPRNGATQILSRSSGYITEKAAAYGRLPAGHPEGYIIAFANTYKN
                     IINTILKKNSGEKPSEEDLDFQTVTDGLNGVKFVHAVIESADNDSKWVTLD"
BASE COUNT         1124 a          757 c          877 g          995 t
ORIGIN      
        1 ctcggcgaga atatgaatta tcggaatggg attccggata atcaaagatt taccgtatat
       61 attctgccga cagccgaaga tatccctccg attcaaaatg tttccgttga actttatgag
      121 gcaaccggtc cttatggtgc caagggagcg gctgaagttg cgacagttgt catcgcaccg
      181 gcaataacat ccgctataga atcgattcgg gatttaaaaa tcaatcggtt acccgtagat
      241 ttcgaacaaa ttctggatgc gaaagagaaa ggctcggttc gctgatggat gtaattcgcc
      301 ccgccagctt gcaggactgt ttacggatct tgaaaaccga acataccata cccgttgccg
      361 gcggaaccga cctgatggta aacctggcgt tgaataaaat ttctccggat aggatcgttg
      421 atatcagtgg gttacaagaa ttgaaagaaa tatctctttc agataatttt ctgaaaatag
      481 gcgcattggt cactttttcg agacttgcgg gaagggaaat cgcacaatac ccattctgca
      541 acgctctgcg agaagccgca gaaaaaatgg ggtcaccgca aattcgcaac cgggcaacga
      601 tcggcgggaa tttggttacg ggttcatcgg ccggtgatgg ttcggtggca atccttgcgt
      661 tggatgccca ggtagaatta accggtttgg acggaattcg aaaagttccg ttatcccttt
      721 tttatagcgg ttatcatcag accgctattc attccgatga gctgctgact gcggtttata
      781 ttcccatccg tcccgggact tcgtcctttc aaaaagtcgg gaaacgcaat gcactcgcaa
      841 ttgcggtcgc cagtttggct atttattcat atatacgtga ttcgcatttc gaacagattt
      901 acattgcaat cggctctgtg gctccgaccc cgattcgggc tgtcaaaaca gatctttttc
      961 ttcagggtca aaaaattacc ggcggagtca ttaaagaagc ggcacgaatc attcaggatg
     1021 aaatcgcacc gattaccgat atccggggaa ctgccgatta tcgaagaaag ctggttgcta
     1081 atcttttaac ggatcaatta caacaactgt cggagtctaa aaattgagat gagtaaagtg
     1141 atgctttcct gtaaaataaa cggcgaagaa atccattccg aaataccatc cgatatgcgt
     1201 ttaattgatt ttctgcgtga ggaaatgaac ctgaccggcg ttaaagaagg gtgcagcgaa
     1261 ggggaatgcg gagcctgtac ggtccttttt aatggcgatc ccgttaattc atgcattttg
     1321 ctggcgattc aagcggaggg ggctgaaata accactattg aaggtatcgc taaggacgga
     1381 aaattatccg aattacaaca agctttcgtt gaccatggtg cggtacaatg cggtttttgt
     1441 actccgggaa tgatcctcgc cgcacaggca ctgttggata aaaatccgca tccgacagaa
     1501 gctgaaatca gacgtgctat ttcgggaaac ttatgccgtt gcaccggtta ccaagcgatc
     1561 gttgatgcca ttcttaccgt cagccaaagg aaataagaat ctttcggcac acttcgacga
     1621 catgaaacct attttcatat gctatgactt tatgatgaaa ataatattga cgaacaaatt
     1681 taatcaatgt attatccagt tatgatatta atttcgtgta cgagcacata acttttactg
     1741 ttcgaagaga tttgttattc aagcatgaca gaatttaagt cacttttcta atttacaagg
     1801 aggaatttta tgggagacag aattgtaact ttatgtacag ctcaatgggc tgatttggaa
     1861 tttgaagaat tatgtaaaac ggcttcatcg atgggttatg acgggattga gatagctacg
     1921 tgggggaaca gtttcgattt agataaagca gcgacagacg atgactatat tcaatttatc
     1981 aagagcactc ttgcgaaata caatttgcaa tgtaaagcat tggcaatgca tatcatcggg
     2041 caatgtgtgg gagatcttcc cgatcctcgc ttgaacaact ttgcaccggc tcggttggcg
     2101 aacaaacccg atgaaatccg agcttgggca atcgaatcga tgaaaaaagg gcctaaagtg
     2161 gcttctaaat taggtgtaaa aatcatcacc tgctttaccg gctctccgat ttggcgctat
     2221 ttatattcat ttccgcaaac gccggaatca atggtcgaag agggctttaa tgaaatcgtc
     2281 cgtctttgga cgccgatttt ggacgaattt aagaaatatg acgtcaaatt tgccttggaa
     2341 gttcatccgg gagaaatcgc attcgactat tattcaaccg caaagattct tgaaaaattt
     2401 aaagataacg acactttcgg aatcaatttt gatcccagcc atttgctttg gcagggaatc
     2461 caaccgcatt tattcctcag agactttatt aaacgggtct atcatgttca nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgaagaaaa taaactttaa aaagtcagat
     2641 gtaaaatttg acgaagcgat agcgaattaa tggggaattt cattatggag aaaaaaacaa
     2701 tcacttacgg gatggttggc ggttcaaacc aagccttcat cggagatatt catcgaaaag
     2761 cgatcgcact ggacggaagg gcaaaattag tcgcgggatg cttctcgtca aatccggatc
     2821 gtaaccggga gacaggtgag acgaatcaca tttcgccgga tcgattatat tccgattatc
     2881 aggctatggc aaaagccgaa tctacccgtc ccgataaaat tgatttcgtt tcgataacga
     2941 ctccgaatca cacccactat gcgatagcaa aagaattctt acttgccggc attaatgtgg
     3001 tttgtgaaaa accgctgtgc tttacggttg agcaagctga ggaactcaaa aaaatttcgg
     3061 aagaaaaagg tttgattttt gcggtcacct atgcctatac cggctataca atggttaagg
     3121 tagccagaga aatgatcgcc aacggaaaaa tcggaaaaat tatcaatgtc aacgctgaat
     3181 acgctcaaga ttggttgctg gatgaaatca gcgaaaatga aaaatcctcg gggaagaaat
     3241 tctccttctg gcggatggat cccgaaaaat ccggtatttc caattgcacg ggggatatcg
     3301 gaactcacat ggaaaacatg gtccattatt tgaccggtct gaaaattaaa cggttgcatg
     3361 cgacgattaa ccgttttggc catgcattgg atctgaatgc aaacataatc gttgaatatg
     3421 aaaatcacat caacggcggg tattggtgtt cacagttagc agccggacat aaaaacggtt
     3481 ttgttgttcg agtcttcggt acagaagggg gaatcgaatg ggcggaggaa ttcccggatt
     3541 acctgaaatt tacacctcgg aacggcgcta ctcagattct gtcccgcagc agcggatata
     3601 taacggagaa agccgccgct tatggcagat taccggcagg tcatccggaa ggatatatta
     3661 ttgcatttgc aaatacttat aaaaatatta tcaatacgat attgaagaag aattccggcg
     3721 aaaaaccgtc cgaagaagat ttggatttcc aaaccgtgac ggatggcctt aacggtgtga
     3781 aattcgtcca cgcagttatc gagagtgccg ataatgattc taagtgggta acgttagatt
     3841 aaacgaagca tca
//
LOCUS       DAOSMM010000110         3737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_24681_length_3737_cov_11.673547, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000110 DAOSMM010000000
VERSION     DAOSMM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24681_length_3737_cov_11.673547"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PK898_09870"
     CDS             <1..595
                     /locus_tag="PK898_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20375.1"
                     /translation="RFDNLTILKKKSAQAETEQGKEKTNVEIKTEMESEAPAHLNQVI
                     TPMNDFYPPQEKVAFTKYFLDFSSPITFWREGRGDIFCLKESDEKACVFIIRHFNSWN
                     SPEDAAVDILDKYSQQVKDFEIYDRHETFSADGYHAYWAGAHFSIDGKNYETGHLFVQ
                     VNNEVFQIMSYATRGIVEKYRQELNHITESFSLSRSE"
     gene            879..1595
                     /locus_tag="PK898_09875"
     CDS             879..1595
                     /locus_tag="PK898_09875"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HOU20376.1"
                     /translation="MTKKSKKTKPDFSLPESGRKRTFILYTENFFELIKLSLIFILFS
                     LPVFTVFASATAMNYVISKIMNDRLVFIWDDFIKAFKSNFLRSTLYGILSSIIFIILI
                     MGWNISSQAATLSSTLRMILKVFYIFLGVILILHTVYFYPIQAWIDLPFRSVVRNAML
                     IQFVAYKTTLKIFLILIILIPFIWIFRPITYFLIPFFSATIVFLLNSYAMQPIQKYLI
                     IKKPEEDPAFNDSDDIHINI"
     gene            complement(1585..>3737)
                     /locus_tag="PK898_09880"
     CDS             complement(1585..>3737)
                     /locus_tag="PK898_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOU20377.1"
                     /translation="EMIARGRYEVNGGVWVECDCNITGGESIIRQFLWGQRYTLQKFG
                     ILSDCFWLPDTFGYSAAIPQIMRGFGIKYFLTTKMAWNDTNRFPYNTFVWEGIDHSQV
                     LAHLFVMDTWADPQGLLERVNGIQYEDGLINKQDCSERLVAFGYGDGGGGPTAEMIET
                     ARRTEDLEGCPKTYYSSVSGFMKHIEETYLHLPRYKGELYLELHRGTLTNKQQIKKNN
                     RKSEVALHNLELLETVQAVQAGRAASVEKTNQLWEILLKNQFHDILPGSCIAVAHDQS
                     IAETNSLLETAAEMTDQILLSDNRSACFDAVFNPLGFEHKTPFEIPTEEMYTDPKGEL
                     KTQQIVNLDNETKLVVSGLSQKPFCRTAFQLEPGISSAETPFLLNGNRLETAFAWIQF
                     DEDGYICSMIDKSNHRELCRGIPFGTFASTEDVPAKWDGWDIDADYELKLKAEKGNLI
                     SREVVSNGAVELRIRSRYKVCRNSEIDVDQIFYAESPLIEYDVRLDWRDPHYLLKVLF
                     DTTISVPFATFDIQFGNIKRTTTRNNTVEQARFEVCCHKYVDLSEPAFGFTVVNDCKY
                     GVGVRDGLIGLSLAKGGTRPDERGDVGVYRFRYGFFPHTGGYCAKEVILPAYCFNYPS
                     LPMRGTNISTEPLLTTDAANVIIETIKPCEDSEKAFIARIYESEGSFTSTKLRAGFQI
                     QSLQVCNMMEQPICDCDPNLVFQPFEIKTIKVKY"
BASE COUNT         1069 a          861 c          721 g         1086 t
ORIGIN      
        1 ccgctttgat aatctcacca tcttaaagaa aaaatccgct caagccgaga ccgaacaagg
       61 caaagaaaaa accaacgttg aaattaagac cgaaatggag agtgaagccc ccgctcattt
      121 gaatcaagtg attactccga tgaatgattt ttatcctcct caagaaaaag tcgcttttac
      181 aaaatacttt ttagattttt caagcccgat cactttttgg agagaagggc gaggagatat
      241 attctgcctg aaagaatccg atgaaaaagc ctgcgtcttt attattcgac attttaacag
      301 ttggaattct cccgaagatg cggctgttga tattctcgat aaatattctc aacaggtaaa
      361 ggattttgaa atttacgacc ggcacgaaac tttttcggca gatggatacc atgcttattg
      421 ggccggtgct catttttcga tcgacgggaa aaattacgaa accggtcatc tgttcgttca
      481 ggttaataac gaagtctttc agattatgtc ctatgcgacg agaggaatag ttgaaaaata
      541 tcgtcaggaa ctgaatcaca taacagaaag tttttcactg tcacgatcgg aatagttgaa
      601 cttgcaaatt ccctcataaa ttaatcgtag cgatgagccg tcggactcta aatagaaaaa
      661 tcgacggctc atcgccgttt ttgtttcatt ataagaatca ggctgccttt ttttcgaaaa
      721 ccaaagaata aatccctgcc cgaatccaaa tcgttgggtt tattcgtagg atcgtaattt
      781 atacgaacga tatcggtaat aattttctcg aggtttcatc tataattcaa taagaaagca
      841 gtcataaccg gttaaaagaa agaatcggag gtatatcaat gacgaagaag tcaaaaaaga
      901 ctaaaccgga tttttctctt cccgaatcag gtcggaaaag aacatttatc ctttataccg
      961 agaacttttt cgaactcatt aagctcagtt tgatttttat tctcttttcg ttgcctgttt
     1021 tcactgtttt tgcatcggcg acagcaatga attatgtcat ctcaaaaata atgaatgatc
     1081 gattggtttt tatttgggat gattttatta aggcattcaa atcaaatttt ttacgaagca
     1141 cgctttacgg cattctttct tcgattatat ttattatatt gatcatgggg tggaatatca
     1201 gcagtcaagc tgccacgttg tcgtcaacac tacgaatgat tctgaaagta ttctatattt
     1261 tcctcggagt aattctgatc ctgcatacag tgtattttta tccgatccag gcttggattg
     1321 atctaccttt tcggtctgtt gtacgaaatg cgatgctgat tcaatttgtt gcttataaaa
     1381 ccaccttaaa aattttcctg attttaatca tcttaattcc gtttatttgg atttttcgtc
     1441 ccatcaccta ttttctgatc ccatttttct ctgcaacaat cgtcttcctg ctgaattcat
     1501 atgccatgca acccattcaa aagtacctga taataaaaaa gccggaggaa gatccggctt
     1561 tcaatgactc ggatgacatt catatcaata tttaactttt atcgttttga tctcaaaggg
     1621 ttgaaaaacc agatttggat cgcaatcaca tatcggttgt tccatcatgt tacaaacctg
     1681 cagcgattga atttggaacc ccgcacgaag tttcgttgaa gtgaaagaac cttcggattc
     1741 ataaatcctt gcgataaaag ctttttcgga atcttcgcag ggtttaatcg tctcgatgat
     1801 aacattcgcc gcatccgtcg tcaacaaggg ttccgtgctg atattcgtcc ctctcatcgg
     1861 tagcgaaggg taattaaagc aataagcggg aaggataact tcttttgcgc agtagccgcc
     1921 ggtatgaggg aaaaagccat aacgaaaacg ataaactcca acatctccgc gttcatccgg
     1981 ccgcgtaccg cctttagcca acgaaagacc gatcaatccg tctcgaacgc cgacgccata
     2041 tttgcaatca ttcaccaccg tgaaaccaaa agccggttca ctgagatcga cgtatttatg
     2101 acagcacacc tcaaaccgcg cctgctcaac ggtattgttt cgagtggttg tccttttgat
     2161 gttgccgaat tgaatatcaa atgtcgcaaa tggaactgaa atggtcgtat caaacaacac
     2221 ttttagcaaa taatgcggat cgcgccaatc taatctaaca tcatattcga tcaaaggaga
     2281 ttcggcataa aaaatctgat caacgtcaat ttccgaatta cgacagacct tatatcggga
     2341 acgaattctc aattccaccg caccgtttga aacaacctcc cgcgaaatca gattcccttt
     2401 ttccgctttc aatttcaact cataatcggc atcgatatcc cagccatccc attttgccgg
     2461 aacatcttcg gtggaagcaa aagttccgaa cgggatgccc cggcataatt cccggtgatt
     2521 ggatttgtca atcatcgaac aaatatatcc gtcttcatcg aattgaatcc aagcaaaagc
     2581 cgtttccaac cggtttccgt tcagaagaaa cggtgtctca gcggatgaaa taccgggttc
     2641 gagctgaaaa gctgtccggc aaaaaggctt ttgcgataat cccgaaacta ccaatttcgt
     2701 ttcattatcc aaattcacga tttgctgggt tttcaattca cccttcggat ccgtatacat
     2761 ttcttccgta gggatctcaa agggggtttt gtgctcaaac cccaacggat tgaaaaccgc
     2821 gtcaaaacac gcgcttctat tgtcagataa taaaatctga tcggtcattt cagcggctgt
     2881 ctccaagagc gaattcgttt cggcaatgga ttgatcatgg gctactgcga tacaacttcc
     2941 cggtaaaata tcatgaaatt ggtttttcag taaaatttcc cacaattgat ttgtcttctc
     3001 aacggaggca gctctgccgg cttgaacagc ctgaacggtt tcaagcagct caagattatg
     3061 aagcgccact tcacttttcc gattattctt tttaatctgc tgcttgttgg ttagcgtccc
     3121 gcgatgcagc tccagataga gctctccttt gtagcgggga agatgcagat aagtctcctc
     3181 gatgtgcttc ataaaccctg agacagaact gtaataggtt ttggggcaac cttccaaatc
     3241 ctccgttcgc cgtgccgttt cgatcatttc cgcggtcgga ccgcccccgc catccccata
     3301 tccgaaggca accagccgct cgctgcaatc ctgtttattg atcaacccgt cttcgtattg
     3361 aatgccgttg actcgctcca ataatccctg cggatccgcc caagtatcca taacaaataa
     3421 atgagcaagc acctggctat gatcgatacc ttcccaaaca aaggtgttat acggaaatcg
     3481 gttagtgtca ttccatgcca ttttcgtggt caggaaatac ttgatcccga agccacgcat
     3541 gatttgagga attgccgccg aatatccaaa agtatccggc agccagaagc aatcggataa
     3601 tatgccgaat ttctgcaaag tatatcgttg tccccataag aattgccgga taatcgattc
     3661 cccgcctgta atattacaat cacattcgac ccagacaccc ccgttcactt cgtatcgtcc
     3721 gcgggcgatc atttcct
//
LOCUS       DAOSMM010000111         3734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_24707_length_3734_cov_22.435716, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000111 DAOSMM010000000
VERSION     DAOSMM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24707_length_3734_cov_22.435716"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..2243)
                     /locus_tag="PK898_09885"
     CDS             complement(18..2243)
                     /locus_tag="PK898_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOU20378.1"
                     /translation="MDSSKIYRNYLELLKSPVVPQGFPTDQLYQFACTGAIASLSQDI
                     RENPDSKTEAAVSALKEIVSHAAAERFRESALEALKEAALSGNPMCRDALFQLATVEL
                     NPAALKILEEYNLSSPSVDFESAKYFFLKNKNDLLRNDPTLNELTRFYLAAPRSVRQE
                     MQRIARIMLPNWGNIVEFLQDPKAETSAWEKIQSEFLYFSETEKELLIRQLLGQSVDA
                     QKLIADLFLRYDDPALLRACLSNQLEPSRADRKALFYFLSDQWTQYERNDLDYQWIRK
                     AFSEGSDFLRNRLIRHGLKSGHTSWLETSEFSVQTEHVFRPISFSQWESWVTKLIAQS
                     DWERLWQILPIAPVALVPEVITALQRENFEPQSEEERDFFRKLIQICSELPPSIPVPF
                     VRRFYNQTSQPFLLNLSKDGNYLAAAFLNGDIRFWNLGDRTLSSGSLQIPNLSPKAFC
                     FGENAETLASAWSDNVIRIFRFPSAVLLNKLPPAAAPVTNLFLSVDGRRIYATEQPNF
                     LQAWGYPAGIPLDDIKLPGFQIIRSSVCPENNRLVFLDDHQSLFIFDIRKKQTVAEIN
                     PFPPQSVLDTSSELLTCVSLDQHITVWNLNSQMKLSDVQESGFSGKILSIAQLIPGEV
                     AVFGSQIGECAVCSLHSGKHLAEIHGNDPFSSLSAIRINTQDNQLYLADSDGSITQWD
                     LTLLRQMISIFHPETIPNPEKLRTYQQKFFTPAVQKTIALMNILFDWRTRYDIEVEFD
                     E"
     gene            complement(2244..2621)
                     /locus_tag="PK898_09890"
     CDS             complement(2244..2621)
                     /locus_tag="PK898_09890"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HOU20379.1"
                     /translation="MSHISVIKTHLQNREFLIRALEELGYRVVEGDNLSIQANNRKKQ
                     VNFLIEIPYSDPIGFRESKNGYQIVADWFKVLTDRNTFRNQLVQKYTYLYLKSELDKQ
                     GFEISREEVSEKNQIHLVVRRTV"
     gene            complement(2600..>3734)
                     /locus_tag="PK898_09895"
     CDS             complement(2600..>3734)
                     /locus_tag="PK898_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU20380.1"
                     /translation="QLEEVLNKLQLTPGVVLRLSEAGKEKLLQAALGLTASQARRVFA
                     KSIVRRGYLSEEDIDFVSQEKKQIIREIEALEYTDSDESPENVGGLGELKKWLNSREK
                     AFGSEAREYGLPAPKGIALIGIPGTGKSLTAKMIGKLWHLPLLRLDVGALFGSLVGES
                     EERVRRALELVELIAPCILWIDELEKALAHGGLDSGTSTRVFGTILTWMQEKKSPVFV
                     VATANDISTLPPELLRRGRFDEIFFLDLPTEAERREILMVHILKRKRLPEAFDLDRLA
                     KASEGYVGSEIEQAIIDAMYIAFNDGEREFTTEDILAALKRQVPISVSQRENIERLRL
                     WLREGRAQSASFRDTSTAESQFVPIQIELDQPPKNESCPTSLL"
BASE COUNT          982 a          908 c          852 g          992 t
ORIGIN      
        1 cggaaatcgg gtttctttca ttcatcaaat tccacctcta tgtcatagcg ggtacgccaa
       61 tcaaacagta tattcatcaa tgcgatcgtt ttttgtacgg ccggtgtgaa aaatttctgt
      121 tgataggtac gcagtttttc gggattggga attgtttcgg gatgaaagat gctgatcatc
      181 tgcctgagca atgtcaagtc ccattgagtg atgcttccgt cggagtcagc cagataaagc
      241 tgattgtcct gcgtattgat ccggatggct gataaagaag aaaaagggtc gttgccgtga
      301 atttccgcca aatgtttgcc gctgtgcagc gaacaaaccg cgcattctcc gatttgcgaa
      361 ccgaagacgg caacctcgcc gggaatcaat tgagcgattg acaggatctt tccgctaaac
      421 cccgactctt gtacgtccga aagtttcatt tggctgttca gattccaaac ggttatatgc
      481 tgatcaaggg aaacacaagt taatagctcg gatgaagtat ccaataccga ttgcggcgga
      541 aacggattga tctccgcaac ggtttgcttt tttcgaatat caaaaatgaa aaggctttga
      601 tgatcatcca aaaaaaccag ccgattattt tccggacaaa ccgaggatcg gattatctga
      661 aaacccggta atttgatgtc atcaagagga attcccgccg gatatcccca agcttgtaaa
      721 aagttcggtt gttccgtcgc ataaattcta cggccatcga cactcaagaa aaggtttgta
      781 acaggtgcgg cggcgggggg cagtttgttc aacagcactg ccgaaggaaa acgaaaaata
      841 cgaatcacat tatccgacca agccgaggcg agcgtttccg cattttctcc gaaacaaaag
      901 gctttcggac tgagattggg gatttgaagg gaaccggaag ataacgttcg atcgcccaga
      961 ttccagaatc gaatgtcgcc gttcagaaaa gccgccgcca gataattgcc gtctttcgaa
     1021 agattcaaca ggaacggttg cgatgtttga ttataaaatc gacggacaaa cggaacgggt
     1081 atcgatggag gcagttcgga acagatttga atcaatttcc gaaaaaaatc tctttcttct
     1141 tccgattgcg gctcaaaatt ttctcgttgt aaggcggtga taacctccgg gaccaaagcc
     1201 accggtgcaa tcggcagaat ttgccaaagt cgttcccaat cagattgagc gattaatttg
     1261 gtaacccagc tttcccactg cgaaaaagaa atcgggcgaa aaacatgctc ggtctgaacc
     1321 gaaaattccg aagtttcaag ccatgaagtg tgaccggatt tcaagccgtg ccggatcagt
     1381 cggttccgta agaaatccga tccttccgaa aacgctttcc gaatccattg atagtccagg
     1441 tcgtttcgtt catattgtgt ccattggtcg cttaaaaaat agaacaatgc ttttcggtcg
     1501 gcgcgcgagg gttccaattg attggacagg caggctcgga gcaaagccgg atcatcatac
     1561 cgcagaaata gatcggcgat cagcttttgt gcgtctaccg attgtccgag taattgtcga
     1621 atgagcaatt ctttttcggt ttcagagaaa taaaggaatt ccgattggat cttttcccat
     1681 gcggaagttt ccgccttcgg gtcttgcaaa aactcgacaa tgttccccca attcggtaac
     1741 ataatacgcg cgattctctg catttcctgc cggacactgc gtggagcggc taaatagaaa
     1801 cgggttaatt cattcaaagt cggatcgttt cgcagcagat cgtttttatt tttcagaaaa
     1861 aagtattttg cgctttcaaa atcgacggac ggcgaggaca aattatattc ttccaaaatt
     1921 ttcagtgcgg cgggattcaa ctcgacagta gccaactgaa aaagtgcatc gcgacacatc
     1981 ggatttcctg aaagagcggc ttccttaagc gcttccaaag ccgattcacg gaaacgttcc
     2041 gccgcggcat gtgatacgat ttcttttaag gcggatacag cggcttctgt tttcgaatcc
     2101 ggattctcac gaatatcttg tgagagactt gcaatcgccc cggtacaggc gaactgatag
     2161 agctgatccg taggaaatcc ctgcggaacg accggggatt ttagcagttc cagataattc
     2221 cggtaaatct ttgatgaatc catttagacc gtacgacgaa ccaccaaatg aatttggttt
     2281 ttttcactca cttcttcgcg ggatatctcg aatccttgct tatcgagttc ggatttgagg
     2341 tacaagtaag tgtatttttg caccagctga ttgcgaaaag tattccgatc cgtcaggact
     2401 ttgaaccaat ccgccactat ttgataaccg ttcttactct ctcgaaaacc gatgggatcc
     2461 gaataaggga tttctatcag aaaattgacc tgtttttttc gattatttgc ttgaatggat
     2521 aaattatccc cctcgacgac ccgataacca agctcttcca acgcccgaat caggaattcg
     2581 cgattttgca gatgcgtttt tataacagag atgtgggaca tgattcgttc tttggcggtt
     2641 gatccaattc gatctgaatc ggaacaaact ggctttcggc ggtagaggta tcgcggaacg
     2701 aggccgattg tgcccttcct tcccgcagcc acaggcgtaa ccgctcgata ttttctctct
     2761 gtgaaaccga aatcgggacc tggcgtttca atgccgccaa aatatcttcc gttgtgaatt
     2821 cgcgttcccc atcgttgaaa gcgatataca tggcgtcaat aatcgcttgt tcgatttccg
     2881 atcccacata tccttcggaa gcttttgcca atcgatccag atcaaaagct tcgggtagtc
     2941 ttttgcgctt cagaatatgc accatcaata tctcgcggcg ttccgcttcg gtgggcaaat
     3001 ctaagaaaaa gatttcatca aaccgcccgc gtcgcagcaa ttccggcgga agggtcgaaa
     3061 tatcattcgc tgtcgcgacc acaaaaaccg gtgatttctt ttcttgcatc caagtcagaa
     3121 tcgtaccgaa aacacgggta ctggttcccg aatccaagcc gccgtgagcg agcgcttttt
     3181 ccaattcatc aatccaaagg atacagggcg caatcaattc gaccaattcc agcgctcggc
     3241 ggacccgttc ttccgattca ccgaccaaac tgccgaataa agctccgaca tccaaacgca
     3301 gcaacggtaa atgccacagt ttcccgatca tttttgcagt cagacttttt ccggtgcccg
     3361 ggattccgat caatgcaatc ccttttggag cgggcaaacc gtattcacga gcttccgaac
     3421 cgaacgcttt ttcgcgcgaa ttcagccatt tttttaattc acccagtccg ccgacgtttt
     3481 cgggagattc atccgaatcc gtatattcca gcgcttcgat ttctcggatg atctgttttt
     3541 tctcttggct gacaaaatca atgtcttcct cgctcagata gccgcgccgg acgatggatt
     3601 tggcgaaaac acgcctggct tgggatgccg tcaaacccaa agccgcctgc agcagcttct
     3661 cttttccggc ttcacttaat cggagaacca cccccggtgt caactgaagt ttgttcaaca
     3721 cttcttccaa ttga
//
LOCUS       DAOSMM010000112         3664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_25322_length_3664_cov_20.303685, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000112 DAOSMM010000000
VERSION     DAOSMM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25322_length_3664_cov_20.303685"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="PK898_09900"
     CDS             complement(<1..1715)
                     /locus_tag="PK898_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOU20381.1"
                     /translation="MKKSSMICFITFILVMMLSVAVSAAPACTAEDYCDSMLKISEQK
                     SKIPFTGDASWMSVSNALFDIQVEYSDGLEVAVDPDPHLIFMCSLDGVTFDSCEADPF
                     VQVGVYTLRVTAAQNDKLFYLESYADTTLKITDANDVDLDGLTVTYHDSQSDSGKPVE
                     DSAAYAFGDNVTILGNLGLDENGNPSPLTKDGYTFKSWNDAEDLSGHSYPFGETIRIT
                     RDLDLYPEWVAEGQEVNAAESQEPALDVNSPEPTEDQAKQASINDKFQLGEGWFAVGD
                     EAAQDVYRYPETEPEEPQTVQPSGSDKFAIGKGWYPVGDVTFEYPEDFQLPQVTVTAP
                     EQAAQIETAVPNETVDMAAQLNEESEIGAQLSESDTELEAKSPELNLLSAEPQENTTD
                     QCLTGECPAQNTEMTLPHTGFSTRRLTAIREQPAELTYSPLNIQLQVPLLDVNAEVVK
                     VPDEASGWAVEWLGDRAGLLEGSALPGEGISYIAAHNHLNAEEAGPFLFIRDLKENDR
                     IFIRNANGDLMEFSVFANEMYGPDDFTLVQQKAQEYEKTLVLITCENESAEGGYLNRR
                     VVFAKP"
     gene            complement(1850..3103)
                     /locus_tag="PK898_09905"
     CDS             complement(1850..3103)
                     /locus_tag="PK898_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20382.1"
                     /translation="MQKFQPKQLLREWLGIHEPLNDAEFVAGMRPIQTTDLPPLSIQE
                     VIAESQKAWRENPLAHRIISITTQYVIGKGVTFKACDSETQCFLRTFWDHPLNKMDLR
                     IHEWSDELSRSGNLFILISSDVSGMSYARAIPSARIREIRTRDNDCEQAIAYKVIQPA
                     TDFLYDEKWIQAADRLNPTLEPVMIHFAVNRTIGSIWGEPDLAPLLKWLSRYANWLED
                     RVRLNRYRNSFLFVVRTHLAGEAQRLQRQSQLNAVPPVPGSILVTNENEEWSVLSPKL
                     ESMEAKEDGLALKKMIAAGAGIPLHFLAEPESENKASAESAGESTCRHFEQRQQLFLN
                     FLAELLQQVISRAALVNSRIRPEDSIRITGDDISVRDNLALSQAGVNIAEVLSGLKTQ
                     GLLSDDELLRITYRFIGEELQDVEK"
     gene            complement(3193..3507)
                     /locus_tag="PK898_09910"
     CDS             complement(3193..3507)
                     /locus_tag="PK898_09910"
                     /inference="COORDINATES: protein motif:HMM:NF041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CC/Se motif family (seleno)protein"
                     /protein_id="HOU20383.1"
                     /translation="MLKISPEAKAWLEKKGQSAITLVRQVAKVNTCCCSTISYMDAVL
                     GKPQKDPAEYHKETIDGIDVYFQQKIKFPEDRDVTIELSNTLGLKSLKVTGMFQGGAI
                     TA"
BASE COUNT          922 a          932 c          788 g         1022 t
ORIGIN      
        1 ggtttagcaa ataccactcg tcgattgaga tatccgcctt ccgcggactc gttttcgcaa
       61 gtgatcaaaa ccaaagtttt ttcatactcc tgcgcttttt gttgaacgag agtgaaatca
      121 tccggaccat acatctcatt tgcaaaaacg ctgaactcca tcagatcgcc gttcgcgttc
      181 cgaataaaaa tccgatcgtt ctccttcaaa tcgcgaataa acagaaacgg tccggcttct
      241 tctgcgttca aatgattatg agcggcgata taactgattc cttctcccgg taaagcgctg
      301 ccttccaata aacccgcgcg atccccgagc cattcaaccg cccagccgct tgcttcatcc
      361 ggcaccttca ccacttcggc atttacatcc agcaacggaa cctgaagttg aatattgaga
      421 ggggaataag ttaattcggc gggctgttcg cgaattgccg tcaaacggcg agtggaaaat
      481 cccgtatgag gtaaagtcat ttccgtattc tgtgccggac actcccccgt caggcattga
      541 tcggttgtgt tttcctgcgg ttcggcggac aggagattca gttcggggct ttttgcctct
      601 aactcggtat cggattctga aagttgggct ccgatttccg attcttcatt gagttgagca
      661 gccatatcga ccgtttcatt cggaaccgct gtctcaattt gagccgcttg ttcgggagca
      721 gttaccgtca cttgcggtaa ttgaaaatct tccggatatt cgaaggtcac atccccaacc
      781 ggataccagc ctttgccgat ggcaaattta tccgaaccgg acggttgtac ggtctgaggt
      841 tcttccggct cggtttcagg atatcgatag acatcttgag ccgcttcatc accgaccgca
      901 aaccagcctt ctcccaattg gaatttgtcg ttaatcgatg cttgttttgc ctgatcctca
      961 gttggttcgg gactatttac gtcaagggca ggttcctgag attcggcagc gttcacttcc
     1021 tggccttccg ccacccattc cggatataaa tcaaggtcac gagtgatccg gatcgtctcg
     1081 ccaaacgggt aagaatgacc gctcaaatct tcggcatcat tccaactttt aaaagtgtat
     1141 ccgtctttcg tcaacgggct gggattcccg ttttcatcca gacccaagtt tcctaaaatc
     1201 gtcacattat ccccaaaagc ataagctgcg gaatcttcga ccggtttccc cgaatcggat
     1261 tgggaatcat gataagtcac cgtcaatcca tccaagtcga cgtcattggc atcggtaatc
     1321 tttaacgttg tatccgcgta gctttcaagg taaaagagtt tgtcattctg agctgcggta
     1381 acccgaaggg tataaactcc cacctgaaca aagggatccg cttcacaact gtcaaaagta
     1441 accccgtcga gtgaacacat aaaaatcaaa tgcggatcgg gatccaccgc cacttccaat
     1501 ccgtccgaat attcgacttg aatatcaaac aacgcattgc tgacactcat ccaagatgca
     1561 tctcccgtaa acgggatttt cgatttctgc tcggatattt tcagcatgga atcgcaataa
     1621 tcctccgccg tacaagcggg tgcagccgat acggcaacgc ttagcatcat gaccaggata
     1681 aaagtgatga agcaaatcat tgaactcttt ttcataatgt ctccatttcc gatcagaaag
     1741 gagtggggaa agttatttcc tccctccgtg tcctgttcct cataaatcgt tcgaaaccga
     1801 acactgattt aagttgcaag ggacatgcca tctttgaaaa atcaatgatt tatttttcta
     1861 catcttgtaa ttcctctccg ataaatcgat aggtaattct caaaagttca tcatccgaaa
     1921 gaagcccctg cgtttttaac cctgaaagga cttccgcaat attaacgccg gcttgactta
     1981 aagcgaggtt gtctcggacg ctgatatcgt caccggttat tcggatggaa tcttcgggtc
     2041 gaatgcgcga attcaccagc gcagctcggg aaattacctg ttgtagcagc tccgcgagaa
     2101 aattcaggaa aagttgttgg cgttgttcaa aatggcgaca ggtcgattct cccgccgatt
     2161 cggcgctggc tttattttcc gattccggtt cggctaaaaa atggagcgga attcctgctc
     2221 ctgccgcaat cattttcttt agcgccaaac cgtcttcttt tgcttccata ctctcgagct
     2281 tcggagagag gacagaccat tcttcatttt cattggtaac caaaatggaa cccggaacgg
     2341 gcgggacagc gttcagctga ctttgacgtt gcaaacgttg agcctcaccc gccagatggg
     2401 tacgcaccac gaacagaaaa ctgttgcgat agcgattcaa ccgaacccga tcctccaacc
     2461 aattggcata gcgactcagc catttcaaaa gaggtgccaa atccggctct ccccatatcg
     2521 atccgatcgt ccggtttacg gcaaaatgaa tcatgaccgg ttctaaggtc ggatttaatc
     2581 gatcggctgc ctgaatccat ttttcgtcgt acagaaaatc tgttgccggt tgaatcactt
     2641 tataagcgat cgcctgttca cagtcattat cccgcgtccg gatttcacga atccgagcgg
     2701 aaggaatcgc ccgagcataa ctcataccgc ttacgtcgga agaaatcaga ataaagagat
     2761 tgccgctgcg gcttaattcg tcgctccatt cgtgaattcg cagatccatt ttattcaagg
     2821 gatgatccca aaatgtccgc agaaaacact gcgtttcgga atcacaggct ttgaacgtga
     2881 cacctttgcc aatcacatat tgtgtcgtaa tcgatataat tcgatgagcc agcggatttt
     2941 cccgccaggc tttctgtgat tcggcgataa cttcttgaat actcagggga ggaagatcgg
     3001 ttgtttgaat cggtcgcatt cctgcgacga attccgcatc attcaggggt tcatgaatcc
     3061 ccagccattc ccgcagcagt tgtttcggtt gaaatttttg cacgacagct ccttttattt
     3121 taaaaacttt ccttttatgg tagccgtttt gaatcggttt ccatactgcc aataaaggga
     3181 gccgaaaata aattaggcgg taatcgctcc gccttgaaac atgccggtca ctttcagaga
     3241 tttcaaaccg agggtattgc ttaattcgat cgtgacatca cgatcttccg ggaattttat
     3301 cttttgctgg aagtagacat cgatcccgtc aattgtttct ttatgatatt ccgccggatc
     3361 tttctgaggt ttacccaaaa ctgcatccat ataagaaatt gtggaacaac aacaggtgtt
     3421 gaccttcgcc acctgccgta ccaacgttat tgcagattgt cctttctttt ctaaccaagc
     3481 tttggcttcc ggactgattt tcagcataga atgatccttt ccgtcaacat atatcttgtt
     3541 tcattttaat tcataacggt aaaccaaatc gccgatttcg attgttttcc atattgcacc
     3601 ggggaatgtt ttgtttgatg gaccaaccga agaggtcggc gaatctccta tagtatctac
     3661 tctt
//
LOCUS       DAOSMM010000113         3596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_25968_length_3596_cov_16.177633, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000113 DAOSMM010000000
VERSION     DAOSMM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25968_length_3596_cov_16.177633"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1753)
                     /locus_tag="PK898_09915"
     CDS             complement(29..1753)
                     /locus_tag="PK898_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU20384.1"
                     /translation="MKQFRTALIVMITCIVFFSTASLVAADETGYLRVAYPSTSLSTI
                     DQALVSDNSQYVESMNVGVLRQNEFSGALEFGMATDQQMSEDGTVFTYKLLENVPWVR
                     YNPTTDQVEKVLDCDGNVRMVKAQDFVYAVERVLRPSTAADYAYILSLIKGAEEYNTA
                     TEGTVSFDTVGVKALDDYTIQYTFKDPGVFNINILSMWMIMAQPSWIIEGDACNEKLG
                     EKWVETGNYQSYGPFTLKEWVHDSNLTLIKNPYWPGTESVPQAKLPGVHIAILEDSAA
                     LAEFEAGNMDVAKVPISDYDRIVSDPVLSKMAVEVPGDIGTEFLTLNPTVPPTDDVRV
                     RKALSMSVDKQAIVKTLKTGKVASIFIHPALSGAPHEEDFPELGIDYDPEGAKQLIQS
                     YCDEKGIEPKDITISFIYPTREDLKIYVEAIQFMWQNTLGINVNLKNTEWDVFKVERM
                     ESKENVYRSTWTNDYMDANNFTADMFLCGATIQKIADWPSKDCTDVSNPAYQDYAQTV
                     IAAGKETDPAKRAALYAHSEEIIVSQEVIIIPVYYYTGLELRNPKLVATVSNTGNNRW
                     EKWEFKED"
     gene            complement(2007..2810)
                     /locus_tag="PK898_09920"
     CDS             complement(2007..2810)
                     /locus_tag="PK898_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOU20385.1"
                     /translation="MDFFVYLSKFLPTLVYPAGILFISLILALAFSKHPKWQKRMVIF
                     ALIVLFVCGNPFPTALLTRNLENSFPPFDGAQKAKVIVVLGGGTESKEYPRQTVEISG
                     AGDRILYAADLWKEGYAEYLLFGGAYYELLSGEKRSVASDMAEVAESLGIPKDKIILQ
                     EKSLNTYEEAVEDAAVLKKMNVDEVILVTSATHMRRAAGCFEAQGLKVIPAATDYGYT
                     DKSWENLMTLNWEKLYTYIIPQSSNMSSLETALKEYIGIFVYRLRGWME"
     gene            complement(2814..>3596)
                     /locus_tag="PK898_09925"
     CDS             complement(2814..>3596)
                     /locus_tag="PK898_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20386.1"
                     /translation="TFTAVPTATVRPASPTPWPSPTPYQIIIVVVTATPKPTTPVWIT
                     STPIRWVTATPIYWYTATPYWIYRTATPIPYVPPVWNYPTAYTYPTKSGGNTYPIWTP
                     YTPKASSTPKPTVNPASLFEFTYVTEKMSADNIPVKWNYTGMKEYMINYLTADRTLYP
                     GFAMEWTEASKVCNASTCTFEIKSIPDQLLKQGSFYIQLQAKDSNGRIYQSDPIGLQV
                     AGQTTPQVTGTPMSKEPEKKPNFIVRFFRWLFGPIIRLFGGK"
BASE COUNT          986 a          837 c          731 g         1042 t
ORIGIN      
        1 cttcggttca tccgattgga gattaggttt aatcttcttt aaattcccat ttctcccaac
       61 gattattacc ggtattcgaa acggttgcca ccagtttcgg atttcgtaat tccaagccgg
      121 tgtagtaata aaccggaatg atgataactt cttgcgagac gattatttct tccgaatgag
      181 cgtaaagggc agcccgtttt gccggatcgg tctccttccc ggcagcaata acggtttgag
      241 cataatcctg ataggcggga ttactgacat ccgtacaatc cttggaaggc caatcggcaa
      301 ttttctgaat cgtcgctccg cacaagaaca tatctgccgt gaaattattg gcatccatgt
      361 aatcgttggt ccacgttgag cgataaacgt tttctttgct ttccatgcgc tccactttga
      421 atacatccca ttctgtattt tttagattta cattaattcc caacgtattt tgccacatga
      481 attgaatggc ttccacatag attttgagat cttcacgcgt cggataaata aaactgatcg
      541 taatatcctt cggttcgatt cctttttcgt cacaatagga ttgaattaat tgttttgccc
      601 cttcaggatc ataatcgatt ccgagttccg ggaaatcttc ttcatgaggt gcacccgata
      661 atgccgggtg aataaaaatc gaggcgactt taccggtttt taatgttttt acaatagcct
      721 gcttatcaac agacatgctt aatgctttcc gaacacgaac atcatccgtc ggcggaactg
      781 tcggattaag agtcaaaaat tccgtaccga tatcacccgg aacttcaacc gccatcttcg
      841 aaagcacggg gtctgaaacg atacgatcat aatccgaaat cgggaccttt gccacatcca
      901 tattgccggc ttcaaattca gccaaagcgg ctgaatcttc caaaatcgcg atatgaacac
      961 cgggcaattt tgcttgagga accgattcag tcccgggcca atagggattt tttatcaagg
     1021 tcagattgga atcatgtacc cattctttaa gtgtaaaagg accgtaactt tgataattgc
     1081 cggtttccac ccatttttct cccagtttct cgttacaggc atctccctct ataatccatg
     1141 aaggttgagc cataatcatc cacatcgaaa gaatgttgat attaaatacc cccggatctt
     1201 tgaacgtgta ttggatcgta taatcgtcta acgctttcac ccctaccgta tcaaaagaaa
     1261 cagtcccttc ggtagccgta ttatattctt ccgccccttt gatcaggctc aggatataag
     1321 cgtagtccgc agcagtagat gggcggagca ctctttcaac cgcatagaca aaatcctgag
     1381 cttttaccat tcttacattt ccgtcacaat ccaatacctt ttccacttga tccgtagtcg
     1441 gattgtatcg tacccaggga acattctcca acaatttgta ggtgaaaacc gttccgtctt
     1501 ccgacatttg ttgatcggta gccatgccga attccaaggc tcccgagaat tcattttgtc
     1561 tcaggacacc gacattcatt gattcgacat attggctgtt atcgctgacc aatgcctgat
     1621 cgatcgttga aagggacgtg cttggatacg ctacacgtaa atagccggtc tcatcagcag
     1681 cgaccaaaga tgccgttgaa aagaaaacaa tacaggtaat cataacgatt aacgcagtcc
     1741 gaaactgttt catagctttc ctccgatatt tataaattaa acgcctttcg gcatacacat
     1801 tttttaatac agaattttgt aagaattttt tgataataat cttttttaaa aagttaagat
     1861 gatttcaatt acaaaatgat gaacaatagg atttgttcca caggcagttc tttttcagtg
     1921 tcgagggata aaacaggaat ctcgtgtttt cgtaaatgat tgattcggaa actactctct
     1981 cacaggaata aagacgtttt ttatcatcac tccatccagc ctctgagacg ataaacgaaa
     2041 atcccgatat attctttcaa agccgtctcg agagaactca tattagaaga ttgaggaata
     2101 atgtaagtat atagtttttc ccaattgagc gtcatcagat tttcccacga tttatcggta
     2161 taaccataat cggtggcagc cggaatcacc ttcagccctt gagcttcaaa gcatcccgcc
     2221 gcccgtcgca tatgagtggc agaagtcacc aagattactt catccacatt catttttttc
     2281 agaacagcgg catcttccac cgcttcttca taggtattca gtgatttttc ctgcagaatt
     2341 attttatctt tcggaattcc caacgattcc gctacctctg ccatatccga agcgaccgat
     2401 cgtttttcgc ccgataataa ttcataataa gctccgccga ataacaaata ttccgcatat
     2461 ccttccttcc aaagatctgc ggcataaaga atccgatcgc cggcaccgct gatttcgact
     2521 gtttggcgcg gatattcctt ggattccgtc cccccgccca gcacaacgat gaccttcgcc
     2581 ttttgggcgc cgtcaaaagg aggaaaactg ttctcgagat ttctggttaa tagcgcggta
     2641 ggaaagggat ttccgcatac aaataacaca attaatgcaa aaatgaccat ccttttctgc
     2701 cattttggat gtttggaaaa agccaacgcc agaattaggc tgataaacaa aataccggcc
     2761 ggatatacca gcgttggcag gaatttcgag agatagacga aaaaatccat cccttatttg
     2821 ccgccgaata atcgaataat cggtccgaac agccatcgga agaatcttac aataaaattc
     2881 ggcttctttt ccggttcttt tgacatcggt gtgccggtga cctgaggcgt tgtttgtccg
     2941 gccacctgta acccgatcgg gtcgctttga taaattcttc cgtttgaatc tttcgcctga
     3001 agttgtatat agaatgaacc ctgtttcaaa agctgatcgg gaattgattt tatttcgaaa
     3061 gtacacgtag aggcattaca cactttcgaa gcttctgtcc attccatcgc aaatccggga
     3121 tataaagtac gatccgcagt caaataattg atcatatact ctttcatacc ggtgtagttc
     3181 catttcaccg gaatattatc cgccgacatc ttttccgtaa cataagtaaa ttcgaataaa
     3241 gaagccgggt tcacggttgg tttcggagtg cttgaggctt tcggagtata aggagtccaa
     3301 atcggatagg tatttccgcc gcttttcgtc ggataggtat aggctgtcgg atagttccat
     3361 accggcggca cataaggaat gggtgtcgct gtccgataaa tccaatatgg tgtcgccgta
     3421 taccaataaa tcggagtagc cgtcacccaa cgaatcggtg ttgatgtaat ccaaaccggc
     3481 gtggtcggtt taggcgttgc cgtgacaacg acaataataa tttgataagg agtcggagat
     3541 ggccatggag tcggcgaagc gggacgaacg gttgccgtcg gaacggcggt aaaagt
//
LOCUS       DAOSMM010000114         3590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_26023_length_3590_cov_17.956719, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000114 DAOSMM010000000
VERSION     DAOSMM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26023_length_3590_cov_17.956719"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..818)
                     /locus_tag="PK898_09930"
     CDS             complement(141..818)
                     /locus_tag="PK898_09930"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF009299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOU20387.1"
                     /translation="MEFLLDTIDISEIEKCLAIFPISGITSNPSIIKQYGKVDFFSHF
                     KDIRSLIGAERSLHIQMVSEDADGILAEAYSILEKIDHDVFIKIPITEQGLKAIQILK
                     KDDAMVTATAIYTEAQALLATAAGADYLAVYYNRMENMDIDPCHVISATAKLIERNQL
                     KTYLLGASFKNMGQVQKAFEAGANAVTVQPSLLYDALKMPAIQKAVDDFRADWAALYG
                     NVSIAAL"
     gene            complement(845..1183)
                     /locus_tag="PK898_09935"
     CDS             complement(845..1183)
                     /locus_tag="PK898_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20388.1"
                     /translation="MRRGISIFFGMVLIIVGLLKLLPLVGVNVIIDGFNLDTWFPVVY
                     ILIGLLELMNQEASGWFWGIVFLVYGVFKLSQIIGFAIPLINMLNIESLLIPFFVLIY
                     GIKAIATSGE"
     gene            complement(1280..1519)
                     /locus_tag="PK898_09940"
     CDS             complement(1280..1519)
                     /locus_tag="PK898_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOU20389.1"
                     /translation="MSENYSDYEKLKGLIEELDDYINHYHGGSVNLISFDGKVVRVKL
                     GGACEGCPLSPSTLHGWVEGTIRQFFPEIESVESV"
     gene            1751..3016
                     /gene="obgE"
                     /locus_tag="PK898_09945"
     CDS             1751..3016
                     /gene="obgE"
                     /locus_tag="PK898_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU20390.1"
                     /translation="MFVDEAEIYVRSGKGGDGMMHFHREKYVSRGGPDGGDGGRGGDL
                     ILKVDRRLNTLAQFRHISRFIAEDGQNGGPNNMTGRSAEDKIVLVPAGTIVRDAVTND
                     LIGDLVNEGQELIVARGGRGGRGNTHFVTPSYQAPRVAEKGAPAEERRLKLELKLIAD
                     VGIVGVPNAGKSSFLAAVTNARPKIADYPFTTVEPNLGVAELDLDHSLVLSDIPGLIE
                     GAHMGVGLGDSFLRHIQRTKVILHLLDGLAEDPIADFDQTNQEMALFDEKLIEKPQLV
                     AFNKMDIPEAAERWPEVEKFLKEKGYQAMPISAATGQNVQAVLWKLYEMLQNVPEELP
                     TESLPVYLPKEDPRKFEITKDAEGDWVVRGVSIERAAKMTFWEHSGSVRRFQTLLRTL
                     GIEDALREAGVQNGDTVMIGDDFEMEWIE"
     gene            3013..>3590
                     /gene="nadD"
                     /locus_tag="PK898_09950"
     CDS             3013..>3590
                     /gene="nadD"
                     /locus_tag="PK898_09950"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOU20391.1"
                     /translation="MTQDRLGIFGGTFDPPHLGHMILAAEAADQLNLDHVLWMLSPTP
                     PHKRQNIISDFEERAELVSACIEGESRFELSTLENERPGPHYTADTLKILKERFPDSK
                     IVFLIGGDSLQYLPTWHEPQLVMERIDELGVMQRPGEVFDWKELKNILPDIDSKLRMI
                     DAPLLEISSTEIRERVKSGHHYKYYLRDSVYRL"
BASE COUNT         1022 a          808 c          804 g          956 t
ORIGIN      
        1 aaaaaagaaa aacggacctg gtattgcgcc cgcccgtttc aaagaattga tactatcgtt
       61 attaccgggg taactttatt cggattagcg gctttaatcg tcacgtttca tgacggaaca
      121 cgcttttata atccttttat ttagagagca gcgatggaga cattcccata tagcgctgcc
      181 caatcggctc tgaaatcatc gacggctttt tgtatcgccg gcatttttaa tgcgtcataa
      241 agaagggaag gctgcaccgt aacggcattt gccccggctt caaacgcttt ttggacttgt
      301 cccatatttt taaaactggc tccgagaaga taggttttaa gttgattccg ctcaattaac
      361 tttgccgtag ccgatatgac atggcaggga tcgatatcca tgttttccat ccggttgtaa
      421 taaaccgcca gataatccgc ccccgctgcg gtcgctaaca gcgcttgtgc ttccgtataa
      481 atggcggtag cggtgaccat tgcatcatct tttttcagga tttgaatcgc tttcagccct
      541 tgttccgtaa tcggaatttt aatgaaaaca tcatgatcga ttttttccag aatcgaatat
      601 gcctcggcca atatcccatc cgcatcttcc gaaaccattt gaatatgaag agatcgttcc
      661 gctccgatta aagaccgaat gtccttgaaa tgactgaaaa aatctacttt cccatattgt
      721 ttaataatgg aaggattgct cgtaataccg ctgatcggaa aaattgccag gcatttctca
      781 atttcggaaa tatcgatcgt gtccagtaaa aactccattt acctcctttg tccaccggct
      841 ccctttattc tccggatgtc gctattgctt tgataccgta aatcagtacg aaaaacggaa
      901 ttaacagaga ttcgatattc aacatattga tcaagggaat agcaaagccg atgatttgac
      961 tgagtttaaa cacgccataa accaggaaaa caatccccca gaaccaaccg cttgcctctt
     1021 gattcattaa ttcaagtaat ccgatcagga tatatacgac cgggaaccaa gtatccaaat
     1081 taaaaccgtc gatgatgaca tttaccccta ccaacggcaa taatttaagc aaaccgacaa
     1141 tgattaaaac catcccgaaa aaaatggaaa ttcctctacg cataataacg ctcctaactt
     1201 cccgattcga accggagaat atgatccctt cctaaactta ttgatcttga tgcaattttt
     1261 gtgccgaaaa aatcaatcct taaacagatt cgacagattc gatctcaggg aaaaattgcc
     1321 ggatggttcc ttcaacccaa ccgtgtaatg ttgaaggaga taacgggcat ccttcacatg
     1381 cgccccctaa tttcactctg acaacttttc cgtcgaacga aattaaatta accgacccgc
     1441 catggtaatg attgatataa tcatccaatt cttcaatcaa acctttcagt ttttcataat
     1501 ccgaataatt ctcactcaca tctctctcct cttcccgttc tcctattatt gttaatttta
     1561 ccttgttcca ttgatccgat gctttcgaaa agagaatctt ccgaattcaa aataattcaa
     1621 tttttttcac ttcccgagaa acaaatgcgg ataaaaattt gccattccct gcttctatat
     1681 cgcagaatcc aatcgaagga tttatagcgt aaaataaaga gaggttgtca gaaagcgagt
     1741 tagagaatat atgtttgttg acgaagcaga aatttatgtc cgctccggaa agggcggtga
     1801 cggaatgatg cattttcatc gggaaaaata tgtttcccgc ggaggtcccg atggggggga
     1861 tggcggaagg ggcggcgacc tgatcctgaa agttgatcgg cgtttaaata ccttggcaca
     1921 atttcgtcac atttcccgtt ttatcgccga agacggtcaa aatgggggac ccaataatat
     1981 gaccgggcga tccgccgaag acaaaattgt cttggttccc gccgggacaa tcgttcgtga
     2041 tgctgttacc aatgatctca tcggcgattt ggttaatgaa ggacaggaat tgatcgttgc
     2101 ccgcggcggg cgcggcggac gcggaaatac tcactttgtc accccttcct atcaagcccc
     2161 gcgtgtggct gaaaaaggcg cacccgcaga ggaaagaaga ttaaaactgg aattgaaatt
     2221 aattgccgat gtcgggattg tgggggttcc caatgccggt aaatccagtt ttctggcagc
     2281 ggtaaccaat gcccggccta agattgccga ttatccgttt acgacggtcg aaccgaactt
     2341 aggggttgcg gaactcgatt tggatcactc tttggtatta tcggatattc cgggattaat
     2401 cgaaggcgca catatgggtg tggggttggg cgattcattc ctgcgacata tccagcgcac
     2461 gaaagtgatt ctccatttac tggatggcct ggccgaagat ccgatcgcgg attttgatca
     2521 gacgaatcag gaaatggctt tgtttgatga aaaactgatc gaaaagccgc aattggttgc
     2581 tttcaataaa atggacatcc cggaagcggc cgaacgttgg ccggaagtag aaaaattcct
     2641 caaggaaaaa ggatatcaag ccatgccgat ttccgcggca acaggacaga acgtccaggc
     2701 cgtcctttgg aaactttatg agatgctgca aaatgtcccg gaagaacttc caacggaaag
     2761 cttaccggtt tatctgccga aagaagatcc gcgtaagttt gaaatcacca aagacgcgga
     2821 aggcgattgg gtagttcggg gtgtttcgat tgaacgggca gccaaaatga cattttggga
     2881 acacagcggt tctgtaagac gctttcagac acttttacga acactcggca tcgaagatgc
     2941 acttcgggaa gcgggtgtgc aaaacggcga cactgttatg atcggcgatg attttgaaat
     3001 ggaatggata gaatgacaca ggatcgtctg ggaatatttg gtggaacctt tgatccccct
     3061 catttgggcc atatgatctt ggcagccgaa gcggcagacc aactgaatct tgatcatgtg
     3121 ctttggatgt tgtcaccgac accgccccat aagcgacaga atatcatctc cgattttgaa
     3181 gagcgggccg aactggtctc cgcctgcata gaaggagaat cgagatttga actttccaca
     3241 ctcgagaacg aacgcccggg gccgcactac acagccgata cgttgaaaat tttaaaagaa
     3301 cgtttcccgg attcaaaaat cgttttttta atcggtggag attcattgca atatttgccc
     3361 acttggcatg aaccgcaatt ggtaatggaa cggatcgatg aattgggtgt catgcaacga
     3421 ccgggggaag tatttgattg gaaagagttg aaaaatatct taccggatat cgatagcaaa
     3481 ttgcgtatga tcgatgctcc gctgcttgaa atctcatcca ccgaaattcg ggaacgggtt
     3541 aaaagcgggc atcattataa atattatctg agagattccg tttaccggct
//
LOCUS       DAOSMM010000115         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_26820_length_3510_cov_17.916643, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000115 DAOSMM010000000
VERSION     DAOSMM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26820_length_3510_cov_17.916643"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK898_09955"
     CDS             <1..250
                     /locus_tag="PK898_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20392.1"
                     /translation="NFGVFRAFLFFPNCKTREFSDPTIIDHAVNPEEMKLIHSFYQQA
                     ASDQIPGYDRIMQNSFGSVYVRVGMRDALKKDCRCEFP"
     gene            complement(322..873)
                     /locus_tag="PK898_09960"
     CDS             complement(322..873)
                     /locus_tag="PK898_09960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU20393.1"
                     /translation="MSENPYIDPTAHVSAKAKIGKNTKIWINVQIREDAEIGEDCVIG
                     KDVYIDHGVKIGNRVKIQNGVSLFNGVTVEDDVFIGPNAAFTNDYYPRAFNRDWEVSE
                     TLIKKGASIGANATIICRHTLGEYCMIGAGSVITSDVEPYTLVVGNPARAIGKICRCG
                     TRLFNDLVCPKCGFDLKKELELK"
     gene            complement(892..1977)
                     /locus_tag="PK898_09965"
     CDS             complement(892..1977)
                     /locus_tag="PK898_09965"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU20394.1"
                     /translation="MIPIAQPLIGEEEQTAVHEVLESGMLASGNIVRAFEEEFADYLG
                     IEHCITTTSGTTALEVALRALGIGPGDKVLTTPFSFIASTNSIVYTGATPVFADIRPD
                     TFNIDPEKIEEALRADSDIKALLIVHLFGQACNMDQIIPLVEKYHLLLIEDCAQSHGA
                     MWKGKPVGTFGNVGCFSFYPTKNMTTGEGGAIVTHDDNLAETCRLLINHGMAVRYHHD
                     LIGYNYRMTNIAAAIGRCQLRKLDRFNQIRRLNGAILTEGIHHSDITTPFVQDDAFFV
                     YHQYTLRIKNDKRDQFTDYLNANDIGYGIFYPLSIPEQKCYQRFGFNTVWPVTDLIKN
                     QVVSIPVHPGLSEEDLDMIVQVVNKFQ"
     gene            2162..>3510
                     /locus_tag="PK898_09970"
     CDS             2162..>3510
                     /locus_tag="PK898_09970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOU20395.1"
                     /translation="MTQENQVSLEKITSLSKRRGFVYPTSEIYGGLTSSYDYGPLGAE
                     LLRNIRNLWWREMITCREDMIGIDSQILLHPETWVASGHVSAFNDPLVEDKVTHKRYR
                     ADHLIESWLEKNPQDEEVVVAGMGLTEIADFIEKHKVKTPDGNDVFPPKAFNIMFQTS
                     IGVVEGEKMPVYLRGETAQGIFTNFSNILNSTRVQLPFGVGQIGKSFRNEITTGQFIF
                     RTLEFEQAEIEYFFDPEKTDWHELFQDWQSQMWNFIHGTLGIREDKLRWRRHSDQERS
                     FYSKETYDLEYEFPFGFKELWGIAYRTDYDLKQHMDHSGKDLRYRDPNDPSRVFIPHV
                     IEPAVGVNRILMMALCDAYWEDIANNRIVMRFKPNLAPYKAAVFPLVKNKPEIVGKAR
                     EVFHMLAKKMGTNWDDRGNIGKRYFYQDEIGTPFGITIDYQTLEDDTVTVRDRDTMK"
BASE COUNT         1039 a          683 c          736 g         1052 t
ORIGIN      
        1 taatttcggt gtattcagag cgtttttatt ttttcccaac tgtaaaactc gggaattcag
       61 tgatcccacc ataatcgatc atgcggtcaa ccccgaagaa atgaaattga ttcattcttt
      121 ttatcaacaa gctgcttcgg accagattcc cggatatgat cggataatgc aaaattcttt
      181 cggatcggtt tatgttcgag tgggcatgcg agatgcacta aaaaaagact gccgatgcga
      241 atttccctga tgatttttta tacggataaa cactttttcg ttcgagaaga atttatatta
      301 cccacggttt aaaagaattt tctactttag ctctaattct ttttttaaat caaacccgca
      361 ttttggacac accaggtcat taaatagacg agtcccgcaa cggcagattt taccgatcgc
      421 tctcgccgga tttccgacaa ccaaggtata tggttccaca tcagaggtaa ttaccgatcc
      481 tgcgccaatc atacaatatt cgcctaaggt atgtcgacaa ataattgtcg cattggcacc
      541 gatagatgct ccctttttaa ttagtgtttc ggaaacttcc caatcacgat taaaagctcg
      601 agggtaatag tcattcgtaa aagctgcatt aggaccgata aagacatcgt cctctaccgt
      661 aaccccgttg aatagcgata cgccgttttg aattttgacc cgatttccga ttttcacgcc
      721 atgatcgatg taaacatctt tcccgatgac acaatcttct ccgatttccg catcttcgcg
      781 aatttgaaca ttaatccata ttttcgtgtt tttaccgatt tttgctttcg ctgaaacatg
      841 agcagtggga tcaatataag ggttctctga cattcaaacg ctcctaaaaa cttattggaa
      901 tttattgaca acctgaacaa tcatatctaa atcctcttcg gataaaccgg ggtgaaccgg
      961 tatggaaaca acttggtttt ttatcaaatc cgtgacaggc caaacagtat tgaatccaaa
     1021 gcgttgatag catttttgtt ccggaatgga aagcggataa aatattccgt atccgatgtc
     1081 atttgcattc aaataatccg taaattgatc ccgcttatcg ttctttattc gtaaggtata
     1141 ttggtgatag acgaaaaaag catcatcttg aacaaacggc gtggttatat ccgaatgatg
     1201 aattccttcg gtcagaattg caccattcaa cctgcgaatt tgattgaacc ggtctaattt
     1261 tcttaattga catcttccga tcgcggcagc aatattggtc atccgatagt tatagccgat
     1321 taaatcatga tgatatctca ccgccatacc atgattgatt aataatcgac aggtttcagc
     1381 cagattatca tcatgagtga caattgctcc gccttctccg gtggtcatat ttttggtcgg
     1441 ataaaaacta aaacaaccga cattgccaaa tgttccgacc ggctttcctt tccacattgc
     1501 cccatgagac tgagcacagt cttcgattaa taaaagatga tatttttcta caagcgggat
     1561 aatttgatcc atattgcaag cttgaccgaa aagatgaaca atcaacaacg cttttatatc
     1621 cgaatcagct cgaagtgcct cttcgatttt ctccggatca atattaaaag tatccggtcg
     1681 gatatcggca aaaaccggag tcgctccggt atatacaatg gaattggtag aggcaataaa
     1741 tgaaaaaggc gtggttaata ctttatctcc ggggccgata cctaaagctc gcagagcaac
     1801 ttcaagagcg gttgtccctg aggtagtcgt aatgcaatgc tcgattccga gataatcggc
     1861 aaattcctct tcgaatgcac gaacaatatt ccctgatgca agcattccgc tttccaaaac
     1921 ttcatgaaca gctgtttgtt cttcttcacc gatcagtggt tgtgcaattg gaatcatttt
     1981 tttatttcct tggaatagat ttcagctttt tatcaaaaaa attatatcag cgttctgcgg
     2041 aatgaaagaa tttataaaaa gaaatatgta gtgtacttat taataatcga tcataatagc
     2101 aagtttatga tgtcgctatg tgataaaatt tgtgggataa aattcggcaa aggactttaa
     2161 gatgactcaa gaaaatcagg tttcattgga aaaaattact tctctttcta aacgacgggg
     2221 tttcgtttat ccgacttcgg aaatttatgg gggattgacc agttcttatg attacggacc
     2281 tttaggggcg gaattgttac gaaatattcg caatttatgg tggcgagaaa tgatcacatg
     2341 tcgtgaagac atgatcggca ttgattcgca aattctgctc catccggaga catgggtagc
     2401 atcggggcat gtgagtgctt ttaatgatcc gttagtcgaa gataaagtca cccataaacg
     2461 atatcgcgcg gatcatttaa tcgagagttg gttggaaaag aatccacagg atgaagaagt
     2521 agttgttgcg ggaatgggtc tgactgaaat cgcggatttt atcgaaaagc ataaagttaa
     2581 aacgccggat ggaaatgatg tatttccgcc taaagcattt aatattatgt ttcaaacttc
     2641 catcggagtc gttgaaggtg aaaaaatgcc tgtttatctc cgcggagaaa cagcccaagg
     2701 aatttttacg aatttttcga atattttgaa ttccactcgg gttcagcttc cttttggagt
     2761 gggacaaatc gggaagagtt ttcgcaatga aattacgacc ggacaattta tttttcgaac
     2821 tttagaattt gaacaggccg agattgagta tttttttgat cccgaaaaaa cagattggca
     2881 tgagctgttt caagattggc aaagccagat gtggaatttt attcatggga cattgggtat
     2941 ccgggaagat aaattacgtt ggcggcggca ttctgatcaa gaacgttctt tttacagtaa
     3001 agaaacatac gatttggaat atgaattccc ctttggcttt aaagaattat ggggaattgc
     3061 ctatcgaaca gactatgatc tgaaacagca tatggatcac tcggggaaag atttacgata
     3121 tcgtgatcca aatgacccga gtcgggtttt tattccacat gtcattgaac cggcagtcgg
     3181 agtcaaccgg attttgatga tggccttatg cgatgcttat tgggaagata tcgcaaataa
     3241 tcggattgtg atgcgcttca aaccgaattt ggctccttat aaggcagcgg ttttcccgtt
     3301 ggtaaagaat aaaccggaaa ttgtggggaa agcccgcgag gttttccata tgctggctaa
     3361 gaaaatgggg accaactggg atgaccgggg caatatcggt aagcgctatt tttatcagga
     3421 tgaaattggc actccctttg gaattacgat tgactatcaa acattggaag atgatacggt
     3481 taccgttcga gatcgggata ccatgaagca
//
LOCUS       DAOSMM010000116         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_27273_length_3464_cov_17.272221, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000116 DAOSMM010000000
VERSION     DAOSMM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27273_length_3464_cov_17.272221"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..679)
                     /locus_tag="PK898_09975"
     CDS             complement(113..679)
                     /locus_tag="PK898_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU20396.1"
                     /translation="MDPFIDISNTTIESERLILRYWQMGDLSDFYEYASVPGVGEMAG
                     WKHHESMEESKKILSLFMEEKDVFAIVSKAEEKVIGSLGLKKSWAEAFPEYQKLKMKE
                     IGYVLSKDYWGRGLMTEAVRRIIRFCFDTEGIEALTVGHFIDNYRSKRVIEKCGFQYV
                     GSGEFYSSQLQKSFPEMRYIFLNSPNPM"
     gene            1009..1464
                     /locus_tag="PK898_09980"
     CDS             1009..1464
                     /locus_tag="PK898_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOU20397.1"
                     /translation="MIRNFLLLLFLINFSAKPSPTWEIHRVIGVIDGDTIVIENGAEI
                     RYIGIDTPETVHPYKPIECYGPEASERNKDLVLNKFILTERDIKDQDSYGRYLRYVYT
                     CEGSINRILVEEGYAFSYYYPPNLKYYKEYLQCELEAHSHKKGLWFSCY"
     gene            1549..1942
                     /gene="rnpB"
                     /locus_tag="PK898_09985"
     ncRNA           1549..1942
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK898_09985"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1974..2468
                     /locus_tag="PK898_09990"
     CDS             1974..2468
                     /locus_tag="PK898_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU20398.1"
                     /translation="MKIAILSDTHNYLSGIQTALNQLQQEKIDTIIHCGDMTLAETVD
                     AFRDYTIHHVWGNGDLDTIAIRFAIQECQAGSSTSEIYTDVLGGKRIAAIHGHNTRLL
                     QAMIENGHFDYVFHGHTHRRRDEEIGGTRVINPGAIGGAFRSSRSFCILNLETNDLNY
                     YPVG"
     gene            2468..>3464
                     /locus_tag="PK898_09995"
     CDS             2468..>3464
                     /locus_tag="PK898_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOU20399.1"
                     /translation="MKIIVTHEQADFDAVASQLAASLLEPAAVPILPKRINRNVATFL
                     KDFAYNFNFRTDAEIAEETVDSVLVVDTQAISNTAKIKSDTEVCVIDHHQPRLNLPEK
                     WKCLFDRTGSCTTILVEKLQNKSVTIDEIQAVMLLMGIYEDTGNLTYGSTTARDLQAA
                     AWLMNQGADLNILNRYLNQPLSEPQQVLAERLLQNCETLTIMGQQIVISQADARDIND
                     EFSTVAHHLRDMFSPDAIFILLGTKGGIRLIGRATNDRIDVAQIMKHFNGGGHPRAAA
                     GLIPLKKGQELTDVLKNTREKLLEMLPQFITPPITVEQIMSTRPLLIDSQMPMEEI"
BASE COUNT         1014 a          764 c          749 g          937 t
ORIGIN      
        1 atggttttgg aacctgcccc tacgttatca ccgcataaaa aaatcgaacc aattgcatcg
       61 tctcaaatac gactacaaca gagacagttt gattgatgag agaatttcag attcacatcg
      121 gattcggaga attcaaaaag atatatcgca tttccggaaa acttttttgt aattgcgagg
      181 aataaaattc acccgatccg acatactgaa atccgcactt ttcgatgaca cgcttagagc
      241 gataattatc gataaaatgt ccgactgtca aagcttcaat cccttcagta tcaaaacaga
      301 agcggatgat tcgccgcaca gcttcggtca tcaaacctct cccccaataa tctttcgaga
      361 gcacataacc gatttccttc attttcagtt tctgatattc cgggaatgct tcagcccatg
      421 attttttcaa acccagggag ccgataactt tttcttctgc tttggaaaca atcgcaaata
      481 catctttttc ttccataaaa agcgaaagta tttttttaga ttcttccatg ctttcatggt
      541 gcttccaacc ggccatctct ccaactcccg gaacagatgc atattcataa aaatcactca
      601 gatcacccat ctgccaatag cggagaatca gtcgttcgct ttcaatggtt gtgttagata
      661 tatcgataaa aggatccatc aaattcctcg attcgtttca tgtttcttct cctaattgat
      721 gaaattttat ctcagcttat tctcgttttt cctttttccg gaaattgatc acaagaatcg
      781 ttgatccttc tcattttcgt tattgacatc tgtgaattcg ttccgtttta ggagataaac
      841 tttggattat taaaaaaaat aaattatgtt tatataaata aatatatctt aaatgcacaa
      901 tagaaataca atttacgatc tttatcaaga ttggaaagac attcaaaaga aatggaaacc
      961 tgaaatcgaa gccaccagtg aaaaagatgt aaaattatgc tctaaaaaat gatcagaaat
     1021 ttcctacttc tactatttct gattaatttc tccgctaaac caagtccgac atgggaaatt
     1081 catagagtta tcggcgtgat tgatggtgac acgatcgtta ttgaaaatgg cgctgaaatc
     1141 agatacattg ggattgatac tccggaaacc gttcatccct ataaacccat tgaatgttac
     1201 ggtccggaag catctgaaag aaataaggat ttggtactta ataaatttat cctgacggag
     1261 agagatatta aagatcaaga tagttatgga agatatttaa gatatgttta tacctgcgaa
     1321 ggttcaatta accggatcct tgtcgaagaa ggatatgcct tttcttatta ttatccgccg
     1381 aatttaaaat attacaaaga atatttacaa tgcgaattgg aggctcatag tcataagaaa
     1441 gggctctggt tctcatgtta ttgatatata aggtatataa gcctgggaga atttctaaca
     1501 aatttcttac ctttacgcac cgccaaattc agattatgat tatattgtca ggttaatcag
     1561 atgaccgcgg tgcggcgtga tcgcaccgag gaaagtccgc actcctgaga gcaagatgcc
     1621 ggataacatc cggggcgggg caacttgccg gaaaagggcc acagaaaata gaccgcttcg
     1681 cgaaagcgga gcaagggtga aaaggtagtg taagagacta ccggcgttcg tggtgacacg
     1741 gcggccaggt aacccccatc tggagcaagg ccaagtaggg gcgaagcgtg tcgcgtacac
     1801 gcggggatct gctcgatccc ctcgagctcc gggtaggctg ctagagtcgc acggtaacgt
     1861 gcgtcccaga gagatggtca tctcagtggg agaactcgag atctcctctg aacagaatgc
     1921 ggcttataga ttaacctggc attttttgtg gagcctttta tttcgatcat ctaatgaaaa
     1981 ttgcgatcct ttccgatacc cataattatc tttccggaat tcaaaccgcc ctcaatcagt
     2041 tgcaacaaga gaaaatagac acaattatcc attgcggcga tatgacactg gcggaaacag
     2101 tcgatgcctt ccgggattac acgattcacc atgtctgggg aaacggggat ttggatacca
     2161 tcgccatccg cttcgcgatt caggaatgcc aagccggcag ctctacttcc gaaatttata
     2221 cggacgtttt gggcgggaag agaatcgccg ccatccacgg acataatacc cgccttttac
     2281 aggcgatgat cgaaaacgga cattttgatt atgtctttca cgggcatacg catcgcagac
     2341 gggatgagga gatcggagga acccgtgtga ttaatcccgg cgcaatcggt ggagcttttc
     2401 gcagcagccg tagtttttgt attctgaatc ttgaaacgaa cgatttaaat tattatccgg
     2461 tcggctgatg aaaataatag tcactcacga gcaggcggat tttgatgcag tcgcgtctca
     2521 actggcggca tcattgctgg aacccgccgc cgtccccata ttaccgaaac ggataaatcg
     2581 aaatgtcgcg acttttctga aagattttgc ttataacttc aattttcgaa cggatgcgga
     2641 aatcgcggaa gagaccgtcg attcggttct ggtggtggat acccaggcaa tcagcaatac
     2701 ggcaaaaatt aaatcggata ccgaagtttg tgtgatcgac caccaccaac cccgtttaaa
     2761 tcttccggaa aaatggaagt gcctttttga tcgaaccgga tcctgtacga cgattctggt
     2821 ggaaaagctt caaaataaat ctgttacgat cgatgaaatt caagccgtaa tgcttctgat
     2881 gggaatctat gaagataccg ggaatttaac ttacgggagc acaaccgcgc gcgatcttca
     2941 ggctgccgcc tggttgatga atcagggtgc ggatctgaat atcctgaatc ggtatttaaa
     3001 ccagccgctc tcggaacctc aacaagtttt agccgaacgc cttttgcaga attgcgaaac
     3061 attgacaatt atggggcagc agatcgtcat ctcgcaagcg gatgctcgtg atatcaacga
     3121 cgaattttcc accgttgccc accatttacg cgatatgttt tctcccgatg cgatttttat
     3181 tctcttaggg accaaagggg ggattcgcct gatcggccgt gccaccaatg atcgaatcga
     3241 cgtcgcacaa attatgaaac atttcaacgg cggcggtcat ccccgtgccg cggccgggtt
     3301 aattccgctc aaaaagggac aggaattgac cgatgtctta aaaaatacgc gggagaaatt
     3361 attggagatg ctgccgcaat ttatcacccc gccgataacg gtggagcaaa ttatgtcaac
     3421 ccgccctctg ttaatcgatt cccaaatgcc gatggaagaa attg
//
LOCUS       DAOSMM010000117         3406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_27894_length_3406_cov_15.264697, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000117 DAOSMM010000000
VERSION     DAOSMM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27894_length_3406_cov_15.264697"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="tilS"
                     /locus_tag="PK898_10000"
     CDS             <1..797
                     /gene="tilS"
                     /locus_tag="PK898_10000"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU20400.1"
                     /translation="RKNINKTARVIQTDLDWIDDLINQSWEKVVLPDLSDESRIVLNR
                     SLFLELHPAEQARILRKAAFGLRRDARDFGFDAVESGLAFFRNAKRVDQISFPEKLKL
                     LRENELLIIACENTLLNFEKYPQLENEDAVIPLQEGQPVHIGSWIFNAERRIVQQGAF
                     SDLLRLSKEDPYCCLMDSEKVLPPITIRCAKKGERFAPIGMNGKSQKLSDFWINRKLP
                     RQLRSLYPVIADRERILWIPGFQSADPCKITPETVEVLFIQAILTE"
     gene            complement(876..1808)
                     /locus_tag="PK898_10005"
     CDS             complement(876..1808)
                     /locus_tag="PK898_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HOU20401.1"
                     /translation="MKSETPGIREFINSETTFPAAIHAWEIHLINEGRTTNTVKAFLS
                     DVQLLASYIPPDRTVGSVTTRDLNNFLSWMENERKVPCSPKTYSRRVTSIKSFYKWLK
                     TFGVISDDPAEKVIQKSVLSPLPEILTATEYQVVLDAANEFRFGKKPDARPMALVQLL
                     LATGIKKNETLGISPNHIDLEDPDHPVLYIRYDSPANRYKERKIDLPIDWIPVYKEHL
                     QQYPPTKRVFDFSARRLEYILEDIGKAAGLKKHLSFNMCRWTSAVHDYYSNMDPEAIR
                     IKLGVSKIQWREIFMKLKELRDQYAPPPQKTVEA"
     gene            2137..2337
                     /gene="rpsU"
                     /locus_tag="PK898_10010"
     CDS             2137..2337
                     /gene="rpsU"
                     /locus_tag="PK898_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU20402.1"
                     /translation="MATVVLRPNESQDQLLKRFRKKVVKSGVLSTVRRKRWFVSKSEL
                     ERVERKKSMRRMKRRTVSNRPE"
     gene            2379..2633
                     /gene="infA"
                     /locus_tag="PK898_10015"
     CDS             2379..2633
                     /gene="infA"
                     /locus_tag="PK898_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU20403.1"
                     /translation="MAKEEDNKISVEGTIIEALPGTQFRVRLENGHEVLAYLSGRMRK
                     FYIRILLGDRVRVEMSPYDLSIGRIVYRQRKSGENDYEEM"
     gene            complement(2853..>3406)
                     /locus_tag="PK898_10020"
     CDS             complement(2853..>3406)
                     /locus_tag="PK898_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20404.1"
                     /translation="VIFVMILTGCSSSTPTAVSTNTLPAPTEAPTATQPKITDTPTAL
                     PATATEKPAETQSNVEGKTTSAPASSEQPDEATLKKYFSELGLGKIAPGGQLPLSLEK
                     NATLFTAEDQIGLYGNILVECSPRYIIYDTQAKKTVKEGGFPYPLKGGFGGGDAIDFP
                     AGKYEYKVYVDDTLVAVFPFEVK"
BASE COUNT          922 a          696 c          809 g          979 t
ORIGIN      
        1 tccgtaaaaa tataaacaag accgcccggg tgattcagac agatcttgac tggattgacg
       61 atctgatcaa ccaaagttgg gaaaaagtag tgcttcctga tttatcggat gaatctcgca
      121 ttgtcttgaa tcgcagtctg tttttggaac tgcatccggc cgaacaggct cggattcttc
      181 ggaaagcggc tttcgggtta aggcgcgacg cccgcgattt cggcttcgat gccgtggaaa
      241 gcgggttggc attctttcga aatgccaaac gggtggacca aatctcattc cccgaaaaac
      301 tcaaattgct gcgcgaaaat gagctgctga ttatcgcttg cgaaaataca ttgttgaatt
      361 tcgaaaaata tccccagctt gaaaatgagg atgcggtcat tcctcttcaa gagggacaac
      421 cggttcatat cggctcgtgg attttcaacg cggaacgaag gatcgttcaa cagggagcct
      481 tttccgattt gttgcgactg agtaaagaag atccttattg ctgtctgatg gattccgaaa
      541 aggttttgcc tccgatcaca attcgctgcg ctaaaaaagg agaacggttt gcgccgatcg
      601 gtatgaacgg aaaaagccag aaactttccg acttttggat caatcgaaag cttccccgcc
      661 aactgcgatc tctttatccg gtgattgccg atcgtgaacg catcctttgg attcccggtt
      721 ttcaatcggc ggatccatgc aaaataactc cggaaaccgt cgaagttttg ttcattcaag
      781 ctattttgac cgaataagcg ctattttacc ggaaaaccgg tataaaaaaa gccgattccc
      841 tgaattcgaa accgaccttt ttttgcatca gattcttatg cttcaaccgt tttttgcggc
      901 ggaggcgcat attgatctcg cagttctttc aatttcatga aaatttcccg ccattggatt
      961 tttgaaacgc ccaatttgat gcggatcgct tcggggtcca tatttgaata ataatcatgt
     1021 acggcgcttg tccaacgaca catattgaaa gataaatgct ttttcaagcc cgcagctttt
     1081 ccgatatcct ccaaaatata ttcgagcctg cgagcggaaa aatcaaaaac tcgtttagtc
     1141 ggcggatatt gctggagatg ttctttgtaa accggtatcc aatcaatcgg taaatcgatc
     1201 ttccgttctt tgtatcgatt tgcgggtgag tcataacgaa tgtaaaggac gggatgatcc
     1261 ggatcttcca aatcaatatg attgggggag attcccaaag tttcgttttt tttgatcccg
     1321 gtcgccaaca acaattggac caatgccatc ggtcgcgcat ccggtttttt accgaagcgg
     1381 aactcgtttg ccgcatccaa aaccacttga tattccgtgg cggtcaatat ctcgggcaac
     1441 gggcttaaaa ccgatttttg gatcactttt tcagccggat cgtccgaaat gacgccaaag
     1501 gttttcagcc atttgtaaaa ggatttaatc gaagtgacac ggcgggaata cgtcttggga
     1561 ctgcagggga ctttccgttc gttttccatc caggaaagaa aattgttaag atctcgggtg
     1621 gtaaccgagc cgaccgttcg atccggcgga atataggatg ccaaaagctg aacatcgctc
     1681 aaaaacgctt tgacagtatt ggttgtgcgt ccttcattga tcaaatgaat ttcccaggca
     1741 tgaattgctg ccggaaaggt tgtttccgaa ttaataaatt cccgaatccc aggtgtttcg
     1801 cttttcatag gccggataga gttcctctct atagttcgtg tatataaaag ccgtgatcta
     1861 agtatatctt aagaatgtgg tgaaataata atttcgcaat ttttcgtcat caatttgatg
     1921 atatcgtcaa taaaacataa tttcatttgg aagagtaacc gtcatagatt taccgaaata
     1981 atgtgcagct tccaaatcag gatattttca atgtacttga ttaatgatat tggtttgaca
     2041 ggcacgtatt ccgagggtat aatcggtgtg ttttatcgga cgaattatta agtccgaaac
     2101 ggaagtaccc ttggaatacg agggggggtg agatgagtgg caactgtagt attacgtccg
     2161 aatgaatcac aggatcagct actgaagcgc tttcgaaaga aggtcgtgaa aagtggtgtt
     2221 ttaagtactg ttcgacggaa gcgctggttc gtatccaaga gcgaattaga acgtgtggaa
     2281 cggaaaaaat cgatgcgtcg tatgaaacgg cgaacggtaa gcaatcgtcc ggaataaacc
     2341 gaaaggttac ggatgattga tatcagtaag gaaatagaat ggcgaaggaa gaagataata
     2401 agatcagtgt agaaggaacg attattgagg cattacccgg tacacaattt cgggtgaggc
     2461 tggaaaacgg tcatgaagta ttggcatact tatccggcag aatgcgaaag ttttacatcc
     2521 gtattctgtt gggggatcgg gtccgcgttg aaatgtctcc ttatgatctc tccattggtc
     2581 ggattgtata tcgccaacga aaatcgggcg agaacgatta cgaagagatg tgatcaagcc
     2641 tgtttttcta tattggatat aaaaaataaa tcccgaagat tcttcgggat ttattttttt
     2701 accggattct gttatttccg gtaattattc tgatgaaaag agcaggtaat tagaaaaatg
     2761 tgaatgaaat aggtaaatca ttcactgatt tgaaatatcg tttcttctta tgaaatattg
     2821 gcgtatggtt tctctctcct ttttaaatga gtttatttca cttcgaatgg gaaaacagct
     2881 accaaggtat catcgacata aaccttatat tcatattttc ccgccgggaa atcgatcgca
     2941 tcgcctcccc caaatccacc cttcagcggg taaggaaatc cgccttcttt aaccgttttt
     3001 tttgcttggg tatcatagat aatatatcgg ggactgcact caaccaaaat attcccgtaa
     3061 agaccgatct gatcttcggc agtgaagagg gtggcgttct tttccaagct tagaggaagc
     3121 tgtcccccgg gcgcaatttt accgagaccc aattccgaga aatacttttt gagcgtcgct
     3181 tcatccggtt gctccgatga tgccggagca gaggttgttt ttccctcgac gtttgattgt
     3241 gtttccgcag gcttctccgt tgctgttgcc ggaagagctg tgggtgtgtc cgttattttg
     3301 ggctgagtgg cagtgggtgc ttcggtcggt gccggtaaag tgtttgtact gacggctgtg
     3361 ggcgtactgc tgctgcaacc tgtaaggatc ataacgaata tgacga
//
LOCUS       DAOSMM010000118         3392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_28052_length_3392_cov_18.086904, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000118 DAOSMM010000000
VERSION     DAOSMM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28052_length_3392_cov_18.086904"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..108)
                     /locus_tag="PK898_10025"
     tRNA            complement(35..108)
                     /locus_tag="PK898_10025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Val,seq:gac)
     gene            243..887
                     /locus_tag="PK898_10030"
     CDS             243..887
                     /locus_tag="PK898_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20405.1"
                     /translation="MTKKSNISLIIFILIIAGILSGCSNLPKNPTSTPTMSEEEMVKA
                     AEGTALAVFSMTQTQDAILHPSATPEPTSTPTPAFTATNPAPVIPPTATEPVRPYLSV
                     GYKSCYVKNISGDQDHITPYDRLYLEVCFTNDGSGTWNQNYFAQVTKNDGGNTSPLSV
                     TLGKNVAPNEKACFSFNQNMAGYELGQHYSTFALTTDGGAIVGEGYVSCYWTVY"
     gene            913..1617
                     /locus_tag="PK898_10035"
     CDS             913..1617
                     /locus_tag="PK898_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20406.1"
                     /translation="MNKNILILSVIILTVAIFAIIGAAVQPQLSLDEQNIIVDSVIQT
                     INPQALADSIGATVEARVLTKIGLIPDTSVPTATAPSATLPVLSATVGIAENLIASVT
                     PESYQGLHAKFVNSYAYTEGGDNGNQKTFETEYTPNTLFTFDVVFENDGTVNWPPQVE
                     MRNTGSVSTYTGHRPNVIVDTSNNPVKPGDRLGFSISAHGSEELGWHTFYFQLYDAVS
                     GVPIPGGYGYFSYLAK"
     gene            1666..2061
                     /locus_tag="PK898_10040"
     CDS             1666..2061
                     /locus_tag="PK898_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20407.1"
                     /translation="MITGIEENIFLAALILLLIILFMLSRIYKHEDRFIFWLTLLFSV
                     GAIFSLSGLLFIQLGPEFTGLPDHSPDAQKTLEYFRMLLFFSWILIGILFSKAFNTPM
                     KQYRRILLGMILSFPVTTIAGNFIYQLIL"
     gene            2107..2676
                     /locus_tag="PK898_10045"
     CDS             2107..2676
                     /locus_tag="PK898_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20408.1"
                     /translation="MAQDPWLTVDFIWKISGFTVILLIISWIFDHFKTDSRLTRRTVL
                     STGVVVLGYLFFRFLTVPNIEFNETAISVLFLISWILISFLIYKAVKPDMQKILKGIF
                     SALISFGISISFFNLLIPFKNLSADRQIVNSEYALWVPVIIWIILSGLFYAAFNSGMN
                     RFLRLILGMVCGLAFTAILQPPLLQFLNG"
     gene            2683..3075
                     /locus_tag="PK898_10050"
     CDS             2683..3075
                     /locus_tag="PK898_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20409.1"
                     /translation="MPIENLILLEMIAGWSIVTCLVYELSLSISKHQIIRVLFSLIIS
                     LGFIFLVWAVFFSGKVNWNVEEGFIGISDENIFRFVFILLWICVGFLFSKAFNPSMHP
                     TLRLLLGLICSFLVTNYFMETLYLVYFS"
     gene            3109..3387
                     /locus_tag="PK898_10055"
     CDS             3109..3387
                     /locus_tag="PK898_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20410.1"
                     /translation="MDENILAIIILAVLIFAVFHLLAKSVYAILRIALAVLLIIEILY
                     YLRLLINIPFLNQINWSFIDQLNLRIIQFFKTIFTFGSDAVFRINTMN"
BASE COUNT          919 a          717 c          679 g         1077 t
ORIGIN      
        1 aaaaatcagg gtcgcccctg attctgattt attgtgagcg gtataggact cgaacctacg
       61 gccttcgcga tgtcaacgcg acgctctaac caactgagct aaccgctcga acagcatgca
      121 ttataaacaa taagctgctt cttgacaagg aaatgtttta aaataatgaa aacgcctgaa
      181 tcattcgata tgatagaatt attttgatag cagatccgga acaagattgg aaggggttta
      241 atatgaccaa aaaaagcaat ataagcctaa ttatctttat cctcattatt gcgggaattt
      301 tatcgggatg cagcaatctg ccaaagaatc cgacttccac gccgacaatg agcgaagaag
      361 aaatggtgaa agcggccgaa ggcacggcgt tggctgtatt cagcatgact caaacacaag
      421 acgctattct tcatccgtcc gctacgccgg aaccgacttc cactccgaca cctgcattta
      481 cagccacaaa tccggctccg gtcattcctc cgacagcgac ggaacccgta cgcccttatt
      541 tatcggttgg ttataaaagt tgttatgtga aaaacatctc cggtgatcaa gatcatatta
      601 ccccttatga caggctgtat cttgaagttt gctttaccaa tgacggatct ggaacgtgga
      661 atcaaaatta ctttgctcag gttaccaaaa atgacggagg gaatacgtct cctctttccg
      721 ttactctggg caaaaatgtc gcaccaaatg aaaaagcctg cttcagtttc aatcaaaata
      781 tggccgggta tgaactcggg cagcattatt caacttttgc attgacaacc gatgggggag
      841 cgattgtggg tgaaggatat gtatcctgct attggacagt ttattaaatc gatctttgca
      901 taaaaggata gtatgaacaa gaacattctg attctttcgg taattatcct gacggttgcg
      961 atctttgcaa tcatcggcgc tgccgttcaa ccgcagctct cactggatga gcaaaatatt
     1021 atcgtcgatt ccgtcattca aacgatcaat ccccaagcgc tggccgattc gatcggagcg
     1081 actgtggagg cacgtgtcct gacgaaaatc ggattaattc cggatacatc cgtacccacc
     1141 gccacggctc cttccgcgac acttcctgtc ctgtccgcta ccgtcggcat cgcagagaat
     1201 ctgatcgcct cggtgacacc ggagtcctat cagggtctgc atgccaaatt tgtcaattcc
     1261 tatgcttata ccgaaggcgg tgataacggg aaccaaaaaa cttttgagac agaatatact
     1321 cccaatacac tttttacttt tgatgttgtc tttgaaaatg acggaaccgt gaattggcct
     1381 cctcaggttg aaatgcgaaa taccggttcg gtcagcacct ataccggtca tcgcccaaac
     1441 gtaattgtgg acacttccaa taatccggtc aaaccgggag accggttggg attctccatt
     1501 tcagcccatg gcagcgaaga gctcggatgg catacatttt atttccaact ttacgatgcc
     1561 gtatccggtg taccgattcc gggaggatat ggctatttca gttatttggc aaaataaaaa
     1621 gcaatcctgt ttaaacgata ccttttggag aaatcagaca gaaaaatgat aacgggcatt
     1681 gaggaaaata tttttttggc ggcacttatc ctcttattga tcattctttt tatgctcagc
     1741 cggatttata aacacgaaga ccgtttcatt ttttggttga cacttctttt ttctgtcggt
     1801 gcgattttct ctctctcggg acttctgttc attcagcttg gtccggaatt caccggacta
     1861 cctgatcatt ctcccgatgc gcaaaaaacg ctggaatact tccgaatgct tttattcttc
     1921 agttggattt tgataggtat tctattttcg aaagcgttta atacaccgat gaaacaatac
     1981 cggcgaattt tgctcggaat gatcctttcc tttccggtta cgaccattgc cggaaatttt
     2041 atttatcaac ttattttatg ataaggagcg ggatgatttt tatcagcctg taaaaaagat
     2101 aacgaaatgg ctcaagatcc ttggctgacc gttgatttta tttggaagat ttcaggattt
     2161 accgttatat tattgatcat cagttggatt ttcgatcatt ttaaaaccga ttcccgtctc
     2221 actcgaagaa ccgttctgag tacaggagtt gtggttttag gttatttatt cttccgtttt
     2281 ctcaccgttc cgaatatcga gtttaatgag accgcgattt ccgtattatt cttaatcagt
     2341 tggatactga tttcattctt aatttataaa gcggtaaagc cggatatgca aaaaatattg
     2401 aaaggaatct tctccgcatt gatttccttc ggaataagta tttctttctt taacctgttg
     2461 attcccttta agaacctgtc tgcggatcgg cagattgtta actcggaata tgcattatgg
     2521 gttccggtca taatttggat cattctcagc ggactttttt atgccgcgtt taattccggc
     2581 atgaatcgtt ttctccgact cattttgggg atggtatgtg gattggcatt taccgccatt
     2641 ttgcaacctc cgcttttaca attcctaaac ggttaaatga ttatgcccat cgaaaacctg
     2701 atcttattgg aaatgatagc cggctggtca attgtgactt gcctggtata tgaactttct
     2761 ctttccatca gcaaacatca aattattcga gtcttgtttt ccctgattat cagtctggga
     2821 tttatttttt tggtgtgggc agtatttttc tccggtaaag taaattggaa tgtcgaagaa
     2881 ggattcatcg gaatatccga tgaaaatatt ttccgcttcg tatttatcct tttatggata
     2941 tgcgtcggat tcttattttc aaaagctttc aatccttcca tgcatccgac tctgagactc
     3001 ctgctcggcc tcatttgttc tttccttgtg accaactatt ttatggaaac attgtatttg
     3061 gtatactttt cttagataca tgtagaaaat tccctaaagg agtgagaaat ggatgaaaat
     3121 atactggcaa ttatcatatt ggcagtgtta atttttgccg tttttcatct cttggcaaaa
     3181 tcggtctatg caatcttgag gattgcttta gcagttcttc taataataga aatcctttat
     3241 tatttaaggt tgttgatcaa catccctttt ctaaatcaaa tcaattggag ttttatcgat
     3301 caattaaatt taagaattat ccaattcttt aaaacgatct ttactttcgg atccgatgcg
     3361 gtttttcgaa taaatacgat gaattaaaca ca
//
LOCUS       DAOSMM010000119         3375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_28229_length_3375_cov_16.725301, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000119 DAOSMM010000000
VERSION     DAOSMM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28229_length_3375_cov_16.725301"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1429
                     /locus_tag="PK898_10060"
     CDS             128..1429
                     /locus_tag="PK898_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU20411.1"
                     /translation="MSRKLFVALIACILLFSMAAVVYAEDVTITMVESLTSPERTAIL
                     REIADKYEAANPGVKIEIISPPLEQADAKITQMLLNKSDVDVVEVRDSTLTQFGTNGW
                     LADLAPYLEKWDEKDTLTSAATQVITQFNHTAYLIPYGFYQRGLYYRKDWFKEAGLDV
                     PKTWQDIYDAGLKLTDPSKNRYGWSFRGGTRGYQYADTVYWAYTGVDKLADPNAGYFL
                     KDGDGKTIFTLPEVKEALHFYKSIYKDISPKDSIAWGFSEMVQGFVGGTTAMLIQDPE
                     VIATCSKDMSEDQWDLAPFPIGPSGEAVFPNGFGGWGMTSFSKHPDVAADFLLFLSNS
                     ENNTYFAKNYSTIPIHINAADMDPYFSEGKFAMYMEMAKQPDVYRYATEPQMYEAFSQ
                     FNSEVDQWYQNYLTDQITDDELLAYLDNYWTEAYKNEGKKW"
     gene            1540..2445
                     /locus_tag="PK898_10065"
     CDS             1540..2445
                     /locus_tag="PK898_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU20412.1"
                     /translation="MKTKQKLAGSKNRFIFFMLLPALILPLIFTYYPMIKGSLMAFQS
                     YNLMNVTNIKWIGLGNFQKLFANTPGNIFYTILKNTAIWVIVSLFFQFTIGFALALLL
                     RKKFRGSSVYQGLIFFPWAVSGFLIGIMWRWMFNGTSGVINDILLRIGIIHSPIAWLA
                     SKDTALLSVIIANIWYGIPFFTIMITAALRGVPQELYEAAEVDGANAVGKFFNITIPS
                     IKSVILLTILMRVIWIFNFPDLIYSMTQGGPGGSSHIITSFMMQKVQSLDYGLASALG
                     LLVILFLTVFTIIYFSVTKFGKLED"
     gene            2449..3294
                     /locus_tag="PK898_10070"
     CDS             2449..3294
                     /locus_tag="PK898_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU20413.1"
                     /translation="MKSRKIKRRILSTIKFIVLGVYLIIVIFPFFWMLMTSLKSSQGE
                     IYAFPVKYFPETPSLVNYKEMLWKGNFGRYMMNSFIVSGVAAVCAVFIGILAGYVITR
                     FKFKGRGTVLLFFLVTQMVPAFIMLAPLYQMLSKFGMINKLSTLMLLYTNMMIPFSVV
                     TLRGFFASIPSEIEEAAQIDGCGRLRALFSVIVPIMLPGIAATFIFAFINSWNELFMA
                     VMFIDVDAYKTIPVGLNALILKYDIKWGEMAAGTIMALIPTIFLFSFAQKYMVEGLSA
                     GAVKG"
BASE COUNT          888 a          666 c          784 g         1037 t
ORIGIN      
        1 tttcggagaa gagaaaatga attttatgac taaagtattg attgacagaa tgcctccgat
       61 tctttatact tttgttgcaa agtttcataa agtttatatg tctatttatc aaagatggag
      121 ggaatatatg agtagaaaat tgtttgttgc cttgatagcc tgcatcttgc ttttttcgat
      181 ggcagctgtt gtatatgccg aagatgtgac tatcacgatg gtggaatcgc tcaccagtcc
      241 ggaaagaacc gcgattttac gtgaaattgc ggacaaatat gaagcggcta accccggcgt
      301 aaaaattgag attatttcac ctcccctgga acaggcagat gcgaaaataa cccaaatgct
      361 gttaaataag agcgatgtcg atgtggttga agttcgggat tcgacattga ctcagtttgg
      421 aacgaacggc tggctcgccg atcttgcccc ttatcttgaa aaatgggatg aaaaggacac
      481 actgaccagc gcggctaccc aggtaattac tcaattcaat catacggctt atctgattcc
      541 ctatggtttc taccagagag gcttgtatta tcgaaaagat tggtttaagg aagccgggct
      601 ggatgtaccc aaaacctggc aggatattta tgatgccggt ttaaagctga cggatccctc
      661 aaagaaccgg tacggatggt cattccgcgg cggaacacgc ggctatcaat atgcggatac
      721 agtatactgg gcttataccg gagtcgataa attggcggat ccgaacgccg gttacttcct
      781 gaaggacggg gacggaaaga caatcttcac cttgccggaa gtgaaagagg cgcttcattt
      841 ttataagagt atctataaag atatttcgcc gaaggactcc atcgcttggg gtttttccga
      901 aatggtacag ggatttgtcg gtggcacaac cgccatgttg attcaggatc ccgaagtgat
      961 cgctacctgc tctaaggata tgtctgaaga tcaatgggat ttggctccgt ttccgattgg
     1021 tccgtcgggt gaagcggtat tccccaacgg cttcggagga tggggaatga cttccttctc
     1081 caaacatccc gatgtcgcag ccgatttcct actgttcctt tctaattcgg aaaataatac
     1141 ctattttgcg aagaattact ccaccattcc gattcatatc aacgcggcgg atatggatcc
     1201 gtatttctcc gaagggaagt ttgccatgta tatggaaatg gcaaaacaac cggatgttta
     1261 tcgttatgcc acagaaccgc aaatgtatga ggccttctca caattcaaca gcgaagtgga
     1321 tcagtggtac caaaactatc tgaccgacca gatcaccgat gacgaattat tggcttatct
     1381 ggataattac tggaccgaag cctataaaaa cgaaggcaag aagtggtaag aattaattat
     1441 ccggaataat tatcagttcg gttaaaatga aagaggctgc cttccacctt ttgtatcagg
     1501 aatgccgagg cagccttctt ttatgccggg aggttcaata tgaaaacaaa acaaaaatta
     1561 gccggtagca agaatcgatt tatttttttc atgctcttgc cggctttgat cttaccgctg
     1621 atttttacct attatccgat gatcaaaggc tctttgatgg catttcaaag ctacaatttg
     1681 atgaatgtca ccaatattaa atggatcgga ttgggtaatt ttcaaaagtt attcgcaaat
     1741 acaccgggga acatttttta taccatctta aaaaataccg cgatatgggt gattgtatct
     1801 ttgtttttcc aatttaccat cggttttgcc ttggctttat tgttgcgtaa aaaatttcgc
     1861 ggttcatccg tttatcaggg attgattttc tttccctggg cggtttcggg attcttgatc
     1921 ggaatcatgt ggcgctggat gttcaacggc acctcgggcg tcattaatga tatcttgttg
     1981 cggatcggaa ttatccattc tccgatcgcc tggttggcga gcaaagatac tgcgctcctt
     2041 tcagttatta tcgccaatat ctggtatggg attccgtttt ttacgattat gattactgct
     2101 gcgttacgcg gtgtaccgca ggaattatat gaagccgccg aagttgacgg cgcgaatgcc
     2161 gtcggaaaat tcttcaacat taccattccg agtatcaaat cggtgatcct gctgaccata
     2221 ttaatgagag tcatctggat ctttaatttt ccggatttga tttattcaat gacgcaggga
     2281 ggaccgggcg gatcttcgca tatcatcacc tcttttatga tgcaaaaagt gcaaagtttg
     2341 gattacgggt tagcttccgc attgggatta ttggtgattt tattcctgac cgtctttacg
     2401 attatctatt tttccgttac caagttcggc aaattggagg attaaacgat gaaatctcga
     2461 aaaataaaac gccggattct ttcgacaatc aaattcattg ttctgggagt ctatctgatt
     2521 attgtcatct ttcctttttt ttggatgctg atgacttcct tgaaatcttc tcagggagaa
     2581 atctacgctt ttccggtaaa gtattttccg gaaacccctt ctctggtgaa ttataaggaa
     2641 atgctttgga aggggaactt cgggcgttat atgatgaatt ccttcattgt ttccggcgtt
     2701 gcggctgtct gtgcggtttt tatcggaatt ttggcgggct atgtgattac gcgatttaaa
     2761 ttcaaaggtc ggggaaccgt tctgttgttt ttcttggtaa cgcaaatggt cccggctttt
     2821 atcatgttgg cacctttgta tcaaatgctc tcgaaatttg ggatgattaa taaattatcg
     2881 acattaatgc tgctttatac caacatgatg atcccatttt ccgtggtgac gttgcgcggc
     2941 ttctttgcct cgattccatc ggaaattgag gaggctgccc aaattgacgg ttgcggcaga
     3001 ctacgggcgt tattttccgt catcgtgccg attatgcttc ccgggatcgc agctactttc
     3061 atttttgctt ttatcaactc atggaatgaa ttatttatgg cggtgatgtt cattgacgtc
     3121 gatgcttata agacgattcc ggtgggactg aatgctttga tattgaaata tgatattaaa
     3181 tggggtgaga tggcagccgg gacaattatg gcgctgatcc ccacaatttt cctgttttct
     3241 ttcgctcaga aatatatggt cgaaggactg tcagccggcg cggtaaaagg ttgatcggag
     3301 aatttataag gagactcgga gaagaatttt atgctcaatg aaatgcaaaa tgccaagatg
     3361 atctataaat tgaaa
//
LOCUS       DAOSMM010000120         3334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_28668_length_3334_cov_7.992986, whole genome shotgun sequence.
ACCESSION   DAOSMM010000120 DAOSMM010000000
VERSION     DAOSMM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28668_length_3334_cov_7.992986"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            714..1829
                     /locus_tag="PK898_10075"
     CDS             714..1829
                     /locus_tag="PK898_10075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOU20414.1"
                     /translation="MLVYWLLPSQFLRNGALIIISFSFIYLMDKKSVFVVIGLTMFSY
                     LAGQWIARSSKRGAVHALCIAALLVVLILFKYLGLLTSVVNGLLSFFRQLPQFHITKL
                     LLPLGISYIVFKHISYLTDVKWGLIEPGRLYEFLLYSSLFTIFIAGPIERFERFKPNI
                     AEMRVRFQWKDMEYGFMRIVVGLFKKLVLADWLGYFINTAWLSPGRYGPTTSVLGIIG
                     FSFQIYFDFAGYSDIAIGASRLLGIHIMENFNNPYLASDIGQFWRRWHISLSDWIRDY
                     IFFPISKWNTSKFWRKICVPVIAMALCGLWHGPAFHFLLWGIWHGLGLAMLQLWNEYK
                     RKHSTVARLAKMPWFNLASMAMTFFYVTIGWFWFRGI"
     gene            2090..3013
                     /locus_tag="PK898_10080"
     CDS             2090..3013
                     /locus_tag="PK898_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20415.1"
                     /translation="MEIIVSKGFHFPSYGVSYKVRYRRDEGVWTNIRKPKARIFNVEG
                     RTVTELNNVGLPGQDIVNLDHPIVLLGSSYIEGFQFPPQKIAGTIFENDLRLENPNST
                     VINLGCSGHDPYDSYFRLKYFEKILDFHTSDIILVLNSDNRAWLARHTQPLQFELPKD
                     FGCINTNQREVLQIYIRNLSSFIELITKGIIKHKAEAGPENEKPAFIDRELASSNENS
                     PLSFTESLKNCLHVFQSQYEGFITVSLCDDISFNTQIDEFCRKEGIAFFVFPLVMPKY
                     RINGAGHLNEEGNELLGHFLSRSYRAFKAKH"
BASE COUNT          891 a          685 c          708 g         1050 t
ORIGIN      
        1 attcatgagg cctatcatcg gccaggagcg tttcgaagtc aaggagcggg gggggggaag
       61 ctcatatacg ccaggcctca tcgtcttgaa cacgatcaag caacggactg ttgcaaatat
      121 taatattttc cttgacttcg cgtttcccct cccttaggat ttaaccggct tcacaggggg
      181 gccgcaaata atttcctatt gattcttttg gggggaaaga atgaaaaaag ccgctattat
      241 tgttttttgc atagtcacta tatcttgtgc gttcaccgat atcccgtcag tgacaaaccc
      301 ggatcacaaa aataaattat atggcagaat tttggtcatc tcgccatttc tggcatttta
      361 tcgatcagaa atcgtttgat atttcgggtg cttcgaaaga taagatcctt cagaaggaat
      421 gtctataagg aacaaacatg caagacaaac taatcaaata tataaaagcg gaatttttag
      481 aagatccgaa gacggaaatc gacctcgaca ctaagctaat ttccagcggg atgattgatt
      541 ccttttcttt ggtatctctc caagcgttca ttaaaaaaga atttaagaaa actatacctg
      601 ctcctcgaat cacaacagag actttcgatt caattcgtca gatgatcgat gtcattcgcc
      661 agttttaaag acttatgatt tccgctgttt acttcatgct tctcggaata tctgtgctcg
      721 tttattggtt gcttccgagc caatttctgc gaaacggagc attaattatc attagttttt
      781 cgtttatata tttgatggat aaaaaaagcg tttttgtcgt catcgggctg accatgtttt
      841 cgtacttggc gggacaatgg atcgcacggt cgagcaagcg gggagcggtt catgcgctct
      901 gtatagccgc tcttctcgtc gtgctgatcc tatttaaata tctaggactc ttgacgagtg
      961 ttgtaaatgg actcctctcc ttctttagac aacttcctca attccatata accaaactgt
     1021 tgcttccctt gggaatatct tatatcgttt tcaaacatat aagctatctc acagatgtca
     1081 aatgggggct gatcgaaccg ggaaggctat acgaatttct cctttatagt agccttttta
     1141 cgattttcat tgctggaccg atcgagcgtt tcgagagatt caagccgaat atcgctgaga
     1201 tgcgcgttcg tttccagtgg aaggatatgg aatatggttt tatgagaatt gtggtcggat
     1261 tatttaaaaa actggttctt gcggattggt tgggatattt cattaatacg gcatggctat
     1321 ctcccggacg atatggccca acgacgtctg ttttgggaat tattggcttt tcatttcaga
     1381 tttattttga ttttgccggt tatagtgaca ttgccatcgg tgcctctaga ctcctgggaa
     1441 tccatattat ggaaaatttc aacaatcctt atttggcttc cgacatcggc caattttggc
     1501 gccgctggca tatttctttg tccgattgga ttcgggatta tatattcttt cccatctcta
     1561 agtggaatac gtccaagttc tggagaaaga tctgtgttcc tgtcatcgcg atggctctct
     1621 gcggactatg gcatggtccg gcgtttcatt tcttattatg ggggatatgg catggtcttg
     1681 gattggcgat gcttcagctt tggaacgaat acaagagaaa acactccacg gttgctcgtt
     1741 tggctaaaat gccatggttc aatctagctt ccatggcaat gacattcttt tacgttacta
     1801 tcggttggtt ttggtttcga ggaatatagt ttgccccaat gaaaaagaaa ctatcgaggt
     1861 tgccgcaatg aattcacgta acgttctagt taataatttt ttccgaaaac cattgtggag
     1921 attttttcta aaatacctcg gactgtttct tctgctgttt ttggccttgg cttcactggc
     1981 attgcacaac atcgtggtcg aagaatcgaa tccgtttttt tatgcgaatt tttaaaatat
     2041 gaaaaaattc gttcttctta tattatctgg gctcctggcg ttcagtatcg tggaaatcat
     2101 cgtttcaaag ggattccatt ttcccagcta cggcgtttcc tataaagtcc gttatcggcg
     2161 cgatgaagga gtatggacta acatccgaaa accaaaagcc agaatattca acgtagaagg
     2221 gagaacggta acggagctga acaatgtcgg ccttcccggt caggacatcg tcaatctgga
     2281 tcacccaatt gttcttctgg gatcgtctta tattgagggt tttcaatttc ctccccagaa
     2341 aatcgccggt actatatttg aaaatgatct ccgcttggaa aatccgaatt ctactgtcat
     2401 caatctcgga tgcagcggtc atgatccata tgattcatat tttcgattga aatatttcga
     2461 aaaaatactg gatttccata ctagtgatat catccttgta ctgaattccg ataatcgggc
     2521 atggttggct cgtcataccc aaccacttca gttcgaatta ccgaaagatt tcggatgcat
     2581 taatactaat caaagagaag tccttcagat ctatatacga aatctttcgt cttttatcga
     2641 gttgataaca aagggcatta tcaaacacaa agcggaggct gggccagaga atgaaaagcc
     2701 tgctttcatc gaccgagagc tcgcttcatc aaatgaaaac tctccattgt ccttcacgga
     2761 gtctttgaag aattgtcttc atgtctttca atcacaatat gaaggattca ttacagtatc
     2821 gttatgcgac gatatttctt tcaatactca gattgatgag ttttgtcgca aggaaggtat
     2881 cgcttttttt gtatttccgc tcgttatgcc aaaatatcgg attaacggcg ccggccattt
     2941 aaatgaagaa ggcaatgagt tgttaggaca ttttctttcc cgtagctatc gggcctttaa
     3001 ggctaaacat taacgagtgt agatgccatc tcgattattg taaaaatctc gaattcttgg
     3061 ttttgctaga cagatcctca atcagcctga tactacgagg tcagaaagtc tgttttgact
     3121 tcgcggaatc agatgctcca tttaatccac ttatcaagat ttatggcgta tcggcggctt
     3181 ttctccggct atcatccggt tactctgatt aaataaggca tagcccggcg caaacgaaag
     3241 tatctcggaa aatcagattg agcgggggaa aaagaacgaa tgacccgtat aggacttgaa
     3301 cctataaccc gcggattaag agtccgctgc tcta
//
LOCUS       DAOSMM010000121         3312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_28901_length_3312_cov_12.435370, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000121 DAOSMM010000000
VERSION     DAOSMM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28901_length_3312_cov_12.435370"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..2636
                     /locus_tag="PK898_10085"
     CDS             273..2636
                     /locus_tag="PK898_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU20416.1"
                     /translation="MIKKENQSVIGENTPRSDGFAKVTGKLEYPSDIHIPGMLYAAVL
                     RSPYPHAEILSIDTSEAEAMGAVCLTYKDIKPIIYNERSVSVPSQTYRDRTILPNKAR
                     HVGEPIAAVAAETEELAFKAVKALKVEYKILEAVFDPEEAMKDGAPQLYDTIYLGDEA
                     VKVHNNIGVIRNINEGDCDAGFAQADRIFERDFELGRIYHAQLETKNAVCKPEPDGTL
                     TVWATAQSIHGTRQMLARALNMPLSKINVKKMPFGGAFGSSIQVNTVIPICAALALKA
                     RKPVKLGSSREEDFYSHNKYPAKFHVKIGVTNDGIFTAAHVKALIDFGAHQVQPLPLL
                     GCMAGWFASLYKYSGNIRFDGTAVYTNKTPCCAMQGYGNPQINFAIESMVDIIAEEMN
                     FSPVDLRLKNYRGIGDEFWGQGPTIRSIIKSCGVEESLVEGRKVFKYDEKIEPSKKTG
                     VKRRGIGLARGFHCSGTGGPKEGEVIDYSSAMIKINEDGSVDLCTPVTDHGGGTLDAL
                     RKIVSETLKVPYDLVGLSPADTMNTGYDVCTHATRGVYCGGGAVLNVARKVKEMLLEV
                     AGRLMDEHACDLELVLDEEAHQGRVQVPGYPQKSMTIGQIAKQAQNNSWGTISYIDSY
                     RQKNCPPCFTTHFVEVEVDTETGIITVPRVLIYGDCGVAMNPDLVKGQLTGSFNRGFA
                     YTVYEKLITDEKTGALKIKGLISDYKLPSTTEMPYVGNMQVELCHTYEPTGPYGAKGI
                     GEAALASVQAAVANAIYNAIGIRFYKLPITPEVVLAALKEKEGASHS"
     gene            2633..>3312
                     /locus_tag="PK898_10090"
     CDS             2633..>3312
                     /locus_tag="PK898_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU20417.1"
                     /translation="MNTVYLEKEYDYVKPKTLAEALEILETKKNVKIFAGGTDLIVKL
                     KIGSPIEMDTMLDINGIEELKKVTSDKEGITIGAAEKISVLEKNEDIINRFPALYEAF
                     ISMASISLRNMATLIGNLCNASPGADTAGPVICYGGSLLIKNHAGERRIKAEDFFAEG
                     GKTVLEPNEIVTSVNIPAPAENTGAAFIKLSRVKADLAKISVSVVLTRENNLVGSCRI
                     AMGSVAGKP"
BASE COUNT         1015 a          631 c          803 g          863 t
ORIGIN      
        1 gagaaagcac aggctataga ttttaataaa taataaacaa actaataatt tacaaaaatc
       61 ctgtttaatt atataagtca ggatttttat ttttaaaccg gcgtcaataa tactcataaa
      121 ttaaatattt acaaattgtt tatcaacatg caatatttta tgataaaacg aaccgattga
      181 taaaaaaata ttatggtttt taggattgaa atagtcatcc attatgctac aatgaaatta
      241 caaggatgcc gttaaattaa aggagtacaa gaatgataaa aaaggagaat caatccgtaa
      301 ttggcgagaa tacaccccgc agtgatggct tcgcaaaagt taccggaaaa ttagagtatc
      361 catccgatat acatatcccg gggatgttat atgccgcagt attacgaagt ccgtatccac
      421 atgccgaaat cctatcaatt gataccagcg aagcggaagc gatgggagcg gtttgtttga
      481 cttataaaga tattaaaccg attatttata acgaaagaag cgtttccgtt ccttctcaga
      541 cttatcgtga tcggacaatt ttaccgaaca aagcccgaca cgtcggtgaa ccgattgcag
      601 ccgtcgctgc cgaaactgaa gaattagctt ttaaagcggt taaagcctta aaggttgaat
      661 ataaaattct ggaagccgtt tttgatccgg aagaagctat gaaggatggt gccccccagc
      721 tttatgacac catttatctc ggtgacgaag ctgtaaaggt tcataataat atcggtgtca
      781 ttcggaatat caacgaaggc gactgcgatg ccggcttcgc gcaggcggat cggatttttg
      841 aacgggattt cgaactggga agaatttatc atgcccaact tgagacgaaa aatgcggttt
      901 gtaaaccgga accggatggg actttgactg tctgggcaac cgctcaatcc attcacggta
      961 cccgccagat gctggcacgg gctttgaaca tgccgctcag taaaattaat gtcaaaaaga
     1021 tgccttttgg tggtgcgttc ggatcatcga ttcaggtaaa cacggttatt ccgatttgcg
     1081 ccgcattggc attaaaagca cgaaaaccgg ttaaattggg ttcatcgcgt gaagaagatt
     1141 tttacagcca taacaaatat ccggcaaaat ttcatgtaaa gataggcgta accaatgatg
     1201 gtatttttac tgccgcccat gttaaggcgt taatcgattt tggcgctcat caagttcaac
     1261 ctctgccttt attaggttgt atggcaggtt ggtttgcttc actttataaa tatagcggga
     1321 atatccgttt tgacggaact gcggtatata caaataaaac gccatgctgc gctatgcaag
     1381 gatatggaaa tccgcaaata aactttgcga tcgagagtat ggtcgatatt attgcggaag
     1441 aaatgaattt ttctccggtt gatctgcgtc tgaagaatta tcgcggaatt ggcgatgagt
     1501 tttggggcca aggcccgacc atccgatcaa ttattaaaag ttgcggcgtg gaagagagtt
     1561 tggtagaagg tcgaaaagtt ttcaaatatg atgaaaaaat tgaaccttcc aagaagacgg
     1621 gtgtaaagcg aagaggtatc ggattagcgc gggggttcca ttgttcagga acaggcggac
     1681 caaaggaagg ggaagtcatc gattacagca gtgccatgat caaaatcaat gaagatggca
     1741 gtgtggattt gtgcacccct gtaacggatc acggcggcgg gacactggat gctctgcgaa
     1801 aaatcgtttc cgaaacattg aaagttcctt atgatttggt cgggctttct cctgccgata
     1861 cgatgaacac cggatatgat gtgtgtacgc atgcgacccg tggagtatat tgcggcgggg
     1921 gtgcggtgct gaatgttgcc agaaaagtga aagagatgct tttagaggtc gccgggaggc
     1981 tgatggatga gcatgcctgt gatttggaat tggtgttgga tgaggaagca catcagggac
     2041 gcgttcaagt accgggatat ccgcaaaaga gcatgacaat cggacaaatc gcaaaacaag
     2101 ctcagaataa cagttgggga acaatttcct atattgacag ttatcgacag aaaaattgtc
     2161 cgccttgttt taccactcac tttgtcgaag tggaagtgga taccgagacg ggtattatta
     2221 ctgtcccacg ggttctaatt tatggcgatt gcggagttgc aatgaatcct gatttggtga
     2281 aaggccaatt gaccggttct tttaaccgag gatttgccta tactgtttat gaaaaattga
     2341 tcacagatga aaagacaggc gcgttgaaaa tcaaaggatt aatcagcgat tacaaattac
     2401 cgtcaacaac cgaaatgccg tatgttggga acatgcaggt cgaattgtgc catacttatg
     2461 agccgaccgg accttatggg gcaaaaggga tcggcgaagc agctttagct tctgttcaag
     2521 ctgccgtcgc taatgccatt tataacgcaa tcgggatccg tttctacaaa ttaccgatta
     2581 caccggaagt agttttggct gctttgaaag agaaggaagg agcaagtcat tcatgaatac
     2641 cgtttacctc gaaaaagaat atgactatgt aaaaccgaaa accttggctg aggcattgga
     2701 aattcttgaa accaaaaaaa atgtcaagat attcgccggt ggaacggatt taatcgttaa
     2761 actgaaaatc ggttcgccga tcgagatgga cacgatgctg gacatcaacg ggattgaaga
     2821 attaaaaaag gttacctccg ataaagaagg aataacgata ggagctgccg aaaagatctc
     2881 tgttttagag aaaaatgaag atatcatcaa tcgctttccg gcgctgtatg aagcgtttat
     2941 ttccatggct tcgatctccc tcagaaatat ggcgacatta atcggaaatc tttgtaatgc
     3001 ctctcccggt gccgataccg cgggacctgt tatctgctac ggcggaagcc tgctgatcaa
     3061 aaatcatgcc ggagaacggc gcataaaagc ggaagatttt tttgctgaag gcgggaaaac
     3121 cgtcttggag ccgaatgaaa ttgttacctc agtaaatatt cccgctccgg cagagaatac
     3181 cggtgcggct ttcattaaac tgagcagagt taaagccgat ttggcaaaaa tcagtgtatc
     3241 cgtagtcctc acaagggaaa acaatcttgt cggatcttgc cggattgcga tgggatccgt
     3301 tgccggcaaa cc
//
LOCUS       DAOSMM010000122         3157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_30735_length_3157_cov_19.016119, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000122 DAOSMM010000000
VERSION     DAOSMM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30735_length_3157_cov_19.016119"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK898_10095"
     CDS             complement(<1..182)
                     /locus_tag="PK898_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20418.1"
                     /translation="MDNLPNSALAKICRTIYRDYPEYQDASPKVTKQGDENFLLIFEK
                     KLKTEDGNTLKLTLRVL"
     gene            complement(226..1575)
                     /gene="ftsZ"
                     /locus_tag="PK898_10100"
     CDS             complement(226..1575)
                     /gene="ftsZ"
                     /locus_tag="PK898_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU20419.1"
                     /translation="MNNQTNNTNNEIVYNKPVIKVIGIGGGGGNAVSRMMDKDISGVE
                     FIAANTDVQALKMNKAPKKILLGPNTSHGMGAGGNPENGEKAALESEAEIRQALAGAD
                     IVFLTAGMGGGTGSGAISVVAKIAREIGAVTISVVNTPFSFESGRRIANARNSLMKLA
                     AYSDTLIAIPNDQLLKIVPKNLSLKESFEIADDVLRQGVLGLSQLLTSTGEINVDFSH
                     IKNMLLNGGGSLLSIGYGKGEDKVSKAIYQALNHPLLENLSIENATGLIVNFTGNEDL
                     SFSEVVEGLTSLQKLTNDRADIIPGQIVDNTMGDEVEVILIVTGIASIPLESLNTQAQ
                     IPIKKPETKPEPETVLFHFTPAEAEKRTDQEETKMQPEFSFHGQGIADIKQSETQKKA
                     DSGKINISPIGDATFDTLMSPLGLSGIDPSSVTFIKDNDEVDSKTDLDIPTFIRRKM"
     gene            complement(1594..3003)
                     /locus_tag="PK898_10105"
     CDS             complement(1594..3003)
                     /locus_tag="PK898_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20420.1"
                     /translation="MQGNFIANSNRFSRLGFHYYNDIDHYDEASLDFWLPKLKAAGTR
                     WIVLFIPERAEIPEIFLRRLINAGIEPIINLQLSLSNPPNPELFRQRTAYYQHCGVHL
                     IHFFSRPNMKSSWSVSDWQRPNLVEHFITVFCGLAEICIANKIIPIFPVLTPGGDYWD
                     IPFLKESLRILRDHYAQSILPNMVFSADAGFQEHSIDWGKGGPAAYPSAGVYSENNPD
                     HRGFHIYEWYQAIIRDILKKTYPMVLFQAGQWSLRTGPFDISSAESRRQFFRALTLVQ
                     QSVQNHSAEDELPSYIVCCNFYKLPTDYVLSGKAEKDEKKKSAFFKTGKTTVPNRDRT
                     SEGKTISPTTINLAIRILNSIFPIILSALSPDLQQLIEKMIAKFKSVFSALFPSGENQ
                     SDYFLIPDVIELLSEDQRQLIQQYLQTTNCKAGRNLNEALTSKKVILVNDKTLYPQNI
                     IKQLQSNNCQVRTLSVSKR"
BASE COUNT          793 a          728 c          726 g          910 t
ORIGIN      
        1 agtacacgta aagtgagttt cagggtattc ccgtcttcgg ttttcaattt tttttcaaaa
       61 atcaataaaa aattctcatc gccttgtttt gtcaccttcg gcgaagcgtc ctgatattcc
      121 ggatagtcac gataaattgt gcggcatatt ttggcgagag cactattcgg taaattatcc
      181 attggtttcc tctttccaaa gatgatacgg cacattcatt ccactttaca ttttccgacg
      241 gataaaggtc ggaatatcaa gatctgtttt ggaatcgacc tcatcattat ccttgatgaa
      301 ggttacagaa gacggatcga ttccgctgag tcccaacgga ctcatcaaag tatcgaatgt
      361 agcatccccg ataggtgaaa tattgatttt ccccgaatca gcctttttct gagtttccga
      421 ttgttttata tccgcgatac cttgcccatg aaaagaaaat tcgggctgca tttttgtctc
      481 ttcctgatcc gtgcgctttt cagcctcggc cggtgtgaaa tggaataaaa cagtttccgg
      541 ttcaggcttc gtttcgggtt ttttgatcgg aatttgggct tgggtgttca acgattctaa
      601 agggattgag gcgattccgg tcacgatcag aatcacctcc acctcatctc ccattgtatt
      661 atcgacgatt tggcccggga taatgtctgc gcggtcgttt gttaattttt gtaagctcgt
      721 caagccctcc accacttcgc tgaaggacag atcttcatta ccggtgaaat tgacgattaa
      781 gccggttgcg ttttcaatag acagattctc cagcaacggg tggttcaaag cttggtaaat
      841 ggctttggac actttgtctt cccccttgcc gtagccgatc gacagcagcg aaccgccgcc
      901 gttcagcaac atattcttaa tatgggaaaa gtccacatta atttcaccgg tgctggttag
      961 caattggctg agtcccaaca caccctggcg caggacatca tcggcgattt caaaagattc
     1021 tttgagagaa aggttcttgg gaacgatttt caaaagttgg tcattgggga tagcgattaa
     1081 agtatccgaa taagccgcta atttcatgag actgttgcgg gcgttggcaa ttcttctgcc
     1141 cgattcaaac gaaaaaggag tattcacgac cgagatggtc accgcaccga tttcccgagc
     1201 gattttggcg acgacgctga tcgcgccgga tccggttccg ccgcccatac cggcggtcag
     1261 gaaaacaatg tccgcgccgg cgagcgcttg ccgaatttcc gcttcgcttt cgagggcggc
     1321 tttttcaccg ttttccgggt tcccgccggc tcccatacca tgagaagtat tgggacccaa
     1381 caggatcttc ttcggtgcct tattcatttt cagcgcctga acatcggtat tggcagcgat
     1441 gaattctacc cctgaaatat ctttatccat catccggctg acggcattgc ccccgcctcc
     1501 tccgatgccg atcactttaa tgaccggttt gttgtataca atttcattat ttgtattatt
     1561 tgtttgattg ttcattggaa ttcctcgcta ttctcaacgt tttgatacgg ataatgtccg
     1621 gacttgacag ttgttacttt gtaattgttt gataatattc tgcggataaa gcgtcttgtc
     1681 gttcaccaga atcacttttt tggaagtcaa ggcttcattg agattccttc ccgccttgca
     1741 gttggtcgtc tgaagatatt gttgaatcag ttgtcgctga tcttcgctca ataattcgat
     1801 cacgtcgggg atcagaaaat aatccgattg attttcgccg cttggaaaca gagcggagaa
     1861 gacggatttg aattttgcga tcatcttttc aatcaattgc tgtaaatcag ggctgagagc
     1921 cgaaaggatg atcgggaaaa tcgaattcaa gattcggatt gccaaattga tggtggtggg
     1981 actgatcgtc tttccttcgg aagtccggtc acggttcggg acggttgttt tccccgtttt
     2041 gaaaaaagcg gattttttct tttcgtcttt ttcggctttc ccgctcagaa cataatcggt
     2101 aggtaattta taaaaattgc agcaaacaat atacgacgga agttcatctt ccgcggaatg
     2161 attttgtacg gattgctgca ccagcgttaa agcccggaaa aattgacgtc gagattccgc
     2221 cgaagaaata tcaaatggtc ccgttcttaa agaccattga cccgcttgga agagaaccat
     2281 cggataggtt ttcttcagga tatcccgaat gatcgcctga taccattcat aaatatggaa
     2341 tccgcgatga tccggattgt tctccgaata aactcccgcg gaaggataag cggccggtcc
     2401 gcccttcccc caatcgatgc tgtgttcctg aaatccggca tcggccgaaa aaaccatatt
     2461 tggtaaaata ctctgcgcgt aatgatcccg cagaattcgc agcgattctt tcaaaaacgg
     2521 aatgtcccaa taatctcctc ccggtgtcag gaccggaaaa atgggaatga ttttgttggc
     2581 gatgcaaatt tccgccaaac cgcaaaaaac ggtgatgaag tgctccacca aattgggacg
     2641 ctgccaatcg ctcacgctcc aagaactttt catattcggc cggctgaaaa agtggatcag
     2701 atggacgccg caatgttggt aataagccgt tcgttgccgg aacagttccg ggttgggagg
     2761 gttggaaagc gacagttgca gattaatgat cggttcgatt ccggcgttga tcaggcgcct
     2821 cagaaaaatt tcgggaatct ctgctctttc cggaatgaag agaacaatcc aacgcgtacc
     2881 ggcagccttt aatttcggca gccagaaatc caggctggct tcatcgtaat gatcgatatc
     2941 attatagtaa tggaagccca agcggctgaa cctgtttgaa tttgcgataa aatttccctg
     3001 catggtttga ccttcatccc gataaatcat accgataatg ttgcaagatt agtgccaata
     3061 agcgcaacgg gcatgaaaaa tgcgtgcgaa ttcgttaatt actgcaggtt catatttcac
     3121 aatcacctgt agtcccatgg ttgagaaaat actgtag
//
LOCUS       DAOSMM010000123         3157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_30737_length_3157_cov_14.070922, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000123 DAOSMM010000000
VERSION     DAOSMM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30737_length_3157_cov_14.070922"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK898_10110"
     CDS             complement(<1..341)
                     /locus_tag="PK898_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20421.1"
                     /translation="MAIKNTFMKKSARKEARTGYLMIAPLMIGLLIFSVRSFFVNFYY
                     SFTNKSAFGNPKFIGLANYEKLFHSDKFYAALENTFLYVLLCVPFVIAISLLLAVLLN
                     KGINLTGFYRTL"
     gene            complement(582..>1292)
                     /locus_tag="PK898_10115"
     CDS             complement(582..>1292)
                     /locus_tag="PK898_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU20422.1"
                     /translation="EYPLAMEIDEPQPSYLQLLVQEGLKIYSDDGLTTDLNDPKAVKP
                     FADIVKLMDDKILPEYKILSDTKATDLFISEKAAMLYVGSWKSSVLDSASFAKNIGLI
                     PMPTRENNHCALGGISYSMSASSKNKEAAWKLISYLSGPESNRIQAEARIEIPAYIAV
                     QDAYGPTFQNIDGSIFIEQSKSAVKYPVHRAIATTTQIISDNAAAIFSRTVTPEEGVT
                     KMAKEVQAAIDEYNATMK"
     assembly_gap    1293..1392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1393..1700)
                     /locus_tag="PK898_10120"
     CDS             complement(<1393..1700)
                     /locus_tag="PK898_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU20423.1"
                     /translation="MLKKVKNVSLILCIVLLLGIFSGANAAAEKTKISFMLWDEVQSP
                     VFETIIGKFMEANPDIEVELQLTPWNQYWTKLDAAAGAGTAPDVFWMNVFMPKYTDAG
                     "
     gene            complement(1753..2760)
                     /locus_tag="PK898_10125"
     CDS             complement(1753..2760)
                     /locus_tag="PK898_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU20424.1"
                     /translation="MDKIDIRFIASQAQVSPATVSRVLNKTKTVSPELRTRVLNTIEK
                     YDYRPNQVARGLINHKTRLIGIVTPKVSNAFHAELISQIEAEADRAHYNVIISNILGN
                     FENEKKTFRIMKDRQVDGIILLHENTPDEMRELEEIVDFPIVLASVRVKDSLLPTVAI
                     DDERAAFEAARYLCQLGHVRIAGVFSDSYSTGTLRRKGFLEGLQSWGIDRNSISLYTT
                     ECSFGGGIAVATEIKNQKNLPTAVFFASDEMAIGAINYFQDHGIRVPEDISVFSFDDI
                     ELSGFVRPRLSTIHQPIQDIGRKATDLLIKLIDEKPLAQREFLLPHQVIVRDSCANPR
                     D"
BASE COUNT          851 a          652 c          624 g          930 t
ORIGIN      
        1 agcgtccgat agaaaccggt caggttaatc cctttattca gtaaaaccgc caaaagcagt
       61 gaaatcgcaa ttacaaatgg aacgcataag agcacataca ggaaggtatt ttccagcgca
      121 gcataaaact tatctgaatg gaataatttc tcataatttg cgaggccgat aaatttcgga
      181 ttcccgaaag cagatttatt ggtaaaactg taataaaaat tcacaaaaaa tgaacgcacc
      241 gaaaaaatca gaaggccgat catcagcgga gcgatcatca gataaccggt tcgagcttct
      301 tttcgagctg atttcttcat aaaagtattt tttattgcca taactgatat accatcctat
      361 gaaaatcaac ggagcggatc aaatcattcg ggtttcaaat attaatgata atgcttctcg
      421 gtatcagaaa aacaccccta aataaactgc cggtccggtt gattctcttg aaataacgga
      481 cacttcggac tttttgagaa gcggtttgat aacgatggca gcaaatatga ataaatgcgc
      541 ggctgccgaa aaatcggcag ccgctgcaat tctcgaagag tttatttcat ggttgcattg
      601 tattcatcga tggcagcctg aacttctttt gccattttcg ttacgccttc ttcaggagtc
      661 accgtcctgg agaaaattgc cgcagcatta tccgaaatta tttgggttgt tgtggcgatc
      721 gcccgatgta ccgggtattt gaccgctgat ttgctttgtt cgataaagat actgccgtcg
      781 atattttgga aagtcggccc gtaagcatct tgtaccgcga tataagccgg aatttcgatt
      841 ctggcttccg cttgaattct gtttgattcg ggtccgctca aataggaaat taatttccag
      901 gctgcttcct tattctttga agaagcgctc atcgaataac tgattccgcc caacgcacag
      961 tgattatttt cgcgggtcgg catcgggatt aatccgatat ttttggcgaa agaagcgctg
     1021 tccaagacag aagatttcca ggatccgaca tacaacatgg cggctttttc ggatatgaac
     1081 aagtcggtgg cttttgtatc cgataagatt ttgtattccg gtaaaatctt atcatccatc
     1141 agtttgacga tatccgcgaa cggttttacc gctttgggat cgttcaaatc ggtcgttaaa
     1201 ccgtcgtcac tgtatatttt taatccttcc tgaaccaaca gttgcaaata agacggttgc
     1261 ggttcatcga tctccattgc gagcggatat tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnattcccgc atccgtgtat ttgggcataa aaacgttcat ccaaaacaca
     1441 tccggagccg taccggcgcc tgctgcggca tccaatttcg tccaatattg attccacgga
     1501 gtaagctgca gctcgacttc gatatcgggg ttcgcttcca tgaatttccc gattatggtc
     1561 tcaaataccg gactttggac ttcatcccat agcataaatg aaatcttggt tttttcggcg
     1621 gcagcatttg caccgctgaa aattcctaaa agcaggacaa tacaaagaat taagcttaca
     1681 tttttaactt ttttcaacat tttttcctcc ttaaatgttt ttatcctttt gataatcggt
     1741 taattgactg aattaatctc tgggattcgc gcaactgtct ctgacaatta cctgatgcgg
     1801 caataaaaac tcccgttgtg ccaaaggttt ctcatcgatt aatttgatta aaagatccgt
     1861 cgcttttcga ccgatatcct gaatcggttg atggatcgtt gaaagacggg ggcggacaaa
     1921 accggataac tcgatatcat cgaaactgaa taccgaaata tcttccggaa cgcggattcc
     1981 atggtcttgg aaatagttaa tcgctccgat tgccatctcg tcactggcga aaaaaacggc
     2041 ggtgggaaga tttttttgat tttttatttc agttgcaacc gctattcctc cgccgaaaga
     2101 gcattcggtt gtatacaacg aaattgaatt ccgatcaatc ccccaacttt gaagtccttc
     2161 taaaaaccct ttccggcgaa gcgtgccggt cgagtaagaa tcgctgaaaa cacccgctat
     2221 tcgtacatga ccgagctgac aaagataacg ggcggcttcg aaagccgctc tttcatcatc
     2281 gattgcaacg gtcggcaaaa gactgtcttt aacccgaaca ctcgccagaa cgatcggaaa
     2341 atcgacgatt tcttctaatt cacgcatttc atccggtgtg ttttcatgta ataaaataat
     2401 tccgtccacc tgccgatctt tcattatccg aaatgtcttt ttttcatttt caaaatttcc
     2461 caagatattg gaaataatca cgttgtaatg agcccgatcg gcttccgctt cgatttggga
     2521 aatcaattcc gcatggaatg cattggaaac cttgggcgtg acgatgccga tcaatctcgt
     2581 tttatggtta atcaaaccgc gagcgacctg attggggcga taatcatatt tttcgatcgt
     2641 attcaaaacc cgtgttctga gctcggggct gactgttttt gttttattta aaacccgtga
     2701 aaccgttgcc ggtgaaacct gagcttgtga tgcgatgaat cgaatatcga ttttatccat
     2761 ttatcttctc tcacctttta aaacaagaaa tcgtatcgga ctttataaaa ttcattatat
     2821 aggaaaacct tttcctgtca agaaaataaa ggaacgaatt taatcacagc ttttttatga
     2881 gtttttacga cggatattca caaataaatt gtgttttaat atgcattctt gaatctttta
     2941 gggttgacag gaaacccttt tcacgtatga ttgagttaat taaagcgaat tcgattttgt
     3001 ccgctctgtg aaaagaggga ggttttatgt tctttcgtta tccgatttcg gatgttcata
     3061 tccatgtgtt tcgtcaggag gatgcgtttg atgttatcaa aatggcagac gaattgtctt
     3121 atgaccaata taatatttta agcgcgacct cattggg
//
LOCUS       DAOSMM010000124         3130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_31110_length_3130_cov_16.904065, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000124 DAOSMM010000000
VERSION     DAOSMM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31110_length_3130_cov_16.904065"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..2353
                     /locus_tag="PK898_10130"
     CDS             119..2353
                     /locus_tag="PK898_10130"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOU20425.1"
                     /translation="MLLDDLLAGLPDNYTQADKDLVEKAYYFAEKAHEGQKRASGEPY
                     FTHCVAVAMILSELKVQSAAIAAGLLHDTVEDTNVTLEDIRANFGDEIAKLVDGVTKL
                     TNIPKTNDYEKNFSPQNKIDNDPISVTDEIELGIQDSAAMRDRKRQLKNETLRKTFIA
                     MVDDVRVIIIKLADRLHNMRTLSHVSKEKQKRIAQETLDIYAPLANRLGIWRIKWELE
                     DLAFRYVHPAEYAQIAENLATTHAIREKEIEEIIERLKTVMESVKIDCEITGRPKHIY
                     SIYTKMIDRNKPLEMLRDLRGVRLIVKDIPSCYAALGQIHTHWRPIPGEFDDYIAVPK
                     DNFYQSLHTAVYYDDGKPLEVQIRTPEMHQNAEYGIAAHWRYKEHTKADPAFEKRLDW
                     LRQLMDWRKDVEDAGEFVEGMKSDVFQDRVFVFTPRGDVIDLPIGSTPIDFAYSVHTE
                     VGHRCRGAKVNGKMVPLSYRLKTGDQVSIITTKQGGPSRDWLNPNLGLVNTQRARSKI
                     RAWFKWQDREQNLTQGKEIVKKDLQRLGLSDIDMNEIAKELDFKNTDDLYVAVGCGDL
                     SSMRVVNKINEGLKVSPLQILQPRPIPTDKTKSSGITVIGTGSIYTTFAKCCNPAPGD
                     DIVGYITKGRGATIHKADCPNIIRVKNRERLINVSWGTPEQRYPVTVTVRAYDRQGLI
                     NDITNLLVSEDVNLENINVKVAHNYANIQIVLDVLNIVQLSRVLTLIEGLPNVVEAYR
                     NKPG"
     gene            2566..2808
                     /locus_tag="PK898_10135"
     CDS             2566..2808
                     /locus_tag="PK898_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20426.1"
                     /translation="MARIRCKYLDCAFLDENYCTAALIELDSDKGCLTYSPVAETPVE
                     DDWEETEEVEDWEDLDEDEKEDDLWIDDEDDEEEEF"
     gene            complement(3021..3091)
                     /locus_tag="PK898_10140"
     tRNA            complement(3021..3091)
                     /locus_tag="PK898_10140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3057..3059),aa:Gln,seq:ctg)
BASE COUNT          981 a          612 c          701 g          836 t
ORIGIN      
        1 ctttcaaaac cttgttatga ataaaacaaa taatttataa ttgttatgcg aaggaattta
       61 atagaaaatt tgacctattt cattgattgt cattgttttc aggaaggtct aaaaaaatat
      121 gttgcttgat gatctacttg ccggtttgcc ggataactat acgcaagccg acaaagattt
      181 ggtggaaaag gcttattatt ttgccgaaaa ggcacacgaa ggccaaaaaa gggcttcggg
      241 tgagccttat tttacccatt gcgtcgctgt tgctatgatt ttgagcgaac tgaaagttca
      301 aagcgccgca atcgccgccg gccttttaca tgacaccgtt gaagatacaa atgtaacctt
      361 agaagatatc cgggcaaatt ttggcgatga aatcgctaaa ttggtcgatg gtgttaccaa
      421 gttgaccaat attcccaaaa caaacgatta cgaaaaaaat ttctcgccgc aaaacaagat
      481 cgataacgat ccgatttccg ttaccgatga aatcgaattg ggtatccaag attcggcagc
      541 gatgcgagat cgcaaacgtc aactgaaaaa tgaaacatta cgaaagacat ttatcgcgat
      601 ggtggatgat gtgcgagtca tcatcatcaa actcgccgat cgtctccata atatgcgaac
      661 cctcagccat gtcagtaagg aaaagcagaa acgaattgct caggagacac tggatattta
      721 tgctccctta gcaaaccgac tcggaatttg gcggataaaa tgggagctgg aagatcttgc
      781 ctttcgatat gttcatccgg ctgaatacgc ccaaattgcc gagaatttag cgacaactca
      841 cgcaattcgc gaaaaggaaa tcgaagagat aatcgagaga ctgaaaacgg ttatggagag
      901 tgtaaaaatt gactgcgaaa ttaccggcag gccgaaacat atttactcga tctatacgaa
      961 aatgattgac cggaataaac cgctcgaaat gttaagagat ttacggggcg ttcgcttgat
     1021 tgtaaaagat attccgtcgt gctatgccgc tttaggacag atccataccc attggcgacc
     1081 gattcccggt gaattcgacg attacatcgc tgtcccgaag gataattttt accaatcttt
     1141 gcataccgcc gtctattatg atgacggcaa accgctcgag gtacaaattc gtaccccgga
     1201 aatgcatcaa aatgccgagt atggtatcgc tgcccattgg cgctataaag agcataccaa
     1261 agccgatccg gcttttgaga aacggttgga ttggttacgc cagctgatgg attggcgaaa
     1321 agatgtggaa gatgccggtg aatttgtgga aggcatgaaa tcggatgtct ttcaggatag
     1381 agtctttgtt tttactcccc gcggagatgt gatcgatctt ccgatcggtt ctacgccaat
     1441 cgattttgca tacagtgtcc atacggaagt cggtcatcgt tgcagaggag ccaaagtcaa
     1501 cggcaaaatg gtcccgttga gctatcgtct gaaaacagga gatcaggttt cgattatcac
     1561 aacgaaacaa ggcggaccga gccgtgattg gctgaatccg aatctcggat tggtgaatac
     1621 ccaacgcgcg cgctcaaaaa tccgagcttg gtttaaatgg caggatcgcg aacaaaatct
     1681 tactcaggga aaagaaatcg taaaaaagga tcttcagcgg ttgggactca gcgatatcga
     1741 tatgaatgaa atcgcaaagg agttggattt taaaaacacc gacgatcttt acgtagcagt
     1801 cggttgcggt gatttatctt cgatgcgggt cgtaaataaa attaacgaag ggctaaaagt
     1861 cagcccgttg caaatcttac agccgagacc cataccgacc gataagacaa aatcttcggg
     1921 cattaccgta atcggaaccg gaagcattta cacgaccttt gcgaaatgct gtaatccggc
     1981 tccgggtgat gatatcgtcg gctatatcac aaaaggacgt ggggcaacga tccataaagc
     2041 ggattgcccg aatattattc gagtgaaaaa ccgagaacga ttaatcaatg tctcatgggg
     2101 aacgccggaa caacgatatc cggttacggt aacggttcgt gcttatgatc ggcagggatt
     2161 aatcaacgat attaccaatt tactcgtcag cgaagatgta aaccttgaaa atatcaatgt
     2221 taaagtcgct cataattatg cgaatattca aattgtgctg gatgtcctta atatcgttca
     2281 gctcagcaga gttctgacct tgattgaagg tttaccgaat gtagttgagg cttatcggaa
     2341 taaacccggt taactcggct cgattaactt tgtaattcaa gttgcttcat cgatcttttt
     2401 ctcctgaata ttcttaatca cttcccaaca aaaaatatta ataaaatttt tatactttcc
     2461 ccctctttta tcctttacat agaaaaccgt atagggtaga atagcgcaaa atttgctagt
     2521 cgacattgaa tactttcggc aaattcatag agaagaggtt taacaatggc aagaattcga
     2581 tgcaaatatc ttgattgcgc ttttttggat gaaaactact gcacggcggc tttaatagag
     2641 ctggactcgg ataaaggttg cctcacttat tcccctgtcg ctgaaactcc cgttgaagat
     2701 gattgggaag aaacggaaga ggttgaagat tgggaagatt tggatgagga tgaaaaagaa
     2761 gacgatcttt ggatagatga tgaggatgat gaggaagagg aattttaatc agtaggactc
     2821 cgattctttt tatacaaaag aattcggcag tctttgccaa tttccacaac tgaatattga
     2881 tccgattgat gagtttaaaa agggatatgc ggtgaaaaat cgcatatccc tttttaagga
     2941 catttactat ataaaaataa atagttttat ttggcctata ataaaaaaat ccgaatgaaa
     3001 tgattcggat tttataaagg ctggggacaa aggattcgaa cctctacata cagattcaga
     3061 gtctgctgtc ctaccattag acgagtcccc aattcttcgg cgttttgtat tctatcacca
     3121 ttaaaaaaat
//
LOCUS       DAOSMM010000125         3096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_31552_length_3096_cov_19.848405, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000125 DAOSMM010000000
VERSION     DAOSMM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31552_length_3096_cov_19.848405"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PK898_10145"
     CDS             complement(<1..720)
                     /locus_tag="PK898_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOU20427.1"
                     /translation="MEQTSEPKQTFMKSATILAGAMILSQIIGLISKGLMGAAFGASA
                     EVDAFLASNRLTETLFNLVAGGALASAFVPWFSSLLDQKDEQSAWKLASDIANLLTII
                     LLLISVACFIFAPQIVRYFLVPGFSLNDPHLQDLTVSLLRIQLPSVIIFGLSGLVMGI
                     LNTHHHFLVPGLAPAMYQLGIIFGVVFLKKPLGIKGLALGAVIGSFLHLLVQIPQLLS
                     LEGKRYSFSLGLRDYRVRNVIK"
     gene            859..1245
                     /locus_tag="PK898_10150"
     CDS             859..1245
                     /locus_tag="PK898_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20428.1"
                     /translation="MKNPLVICCILGILIGYLLGGENYLTYGMVGGLLVGYLLDERNR
                     SAAKKKSTSATRHESIDVSEPTDKTPHSDVSAETNKPLPEQTPDYPIKTISANGTSVP
                     RMNDEQVDDVVARAREEIAKNKEKNN"
     gene            complement(1242..1946)
                     /locus_tag="PK898_10155"
     CDS             complement(1242..1946)
                     /locus_tag="PK898_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20429.1"
                     /translation="MNMKRTIIWAGILFVGIFRSVSAQSSVNLSTLTPLPTYTPAPTY
                     TALPTYTFPIPTEPPTQIVPTKSPTAEKLQIAYVTVTPYLSPTGLVPVALDQEILCGE
                     KMSVKLTYMVPKIQATLSEHFPIGRFLMVYLGMRSLTNDTIGPLKSESFTIAGSLYGR
                     NVTVMLDRGNSDYANSRWETPNLNSKIGKNGLETFLIFDVHPEMTNFELVFTPLTEND
                     EEPLCEVRIPLPIQRH"
     gene            complement(1943..2728)
                     /locus_tag="PK898_10160"
     CDS             complement(1943..2728)
                     /locus_tag="PK898_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20430.1"
                     /translation="MIKIILLVWVIGSLSACTLPSESQIVSTPYDPVSDLVKTIQSMS
                     SRTPYPTGTPYPTQTPYATYSPEEIATFYARNIPAVTPTVRILRETAGQNVILVNPGL
                     WSRDLSKYAFCEDRFAVKIGYRQPYFAYAVGNEVSKSQFLLVNLMVWNISDSTIPLIF
                     HKQFKAVGTYRGEKAEYISYGPAGISASNRWGVPFLSSQAIPPGVEMETWLGFDVNPH
                     ATDWKLVFEADDSSDFSCRFSLDMPQPEFSIQYYKTLESGGTP"
     gene            complement(2786..>3096)
                     /locus_tag="PK898_10165"
     CDS             complement(2786..>3096)
                     /locus_tag="PK898_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU20431.1"
                     /translation="DEEGIWQIPHIDEFESTDATGAGDAFLSGFIYGLYHEADFRTAI
                     LYGNIMGGKAVTEVGCLTASLSEQELLHYADRYLNRIARISEKSHYTSIFPVNPSSSI
                     "
BASE COUNT          930 a          705 c          700 g          761 t
ORIGIN      
        1 tttgatgaca ttgcgaaccc gatagtctct caaacccaat gaaaaagaat agcgtttccc
       61 ttccagcgac aataattggg gaatttgaac gagaaggtga aggaaagagc cgataaccgc
      121 ccccaacgcc aatcccttaa ttcccaacgg ttttttcaga aagacgactc cgaaaataat
      181 tccgagctgg tacattgcgg gagctaagcc cggcacgaga aaatgatgat gggtattcaa
      241 tattcccatc accaatccgc tcaagccgaa aatgatgacc gagggtaatt gaattcgaag
      301 cagcgagaca gtcaaatctt gcagatgcgg atcatttaaa gaaaaacccg ggaccaaaaa
      361 ataacgcaca atctgaggag caaaaataaa acaggcaaca ctgattaaca ataggataat
      421 tgtcagcaga tttgcgatat cggaagctaa tttccaagca ctttgttcgt ctttctgatc
      481 gagcaaagag ctgaaccaag ggacaaaagc acttgccaaa gctcccccgg caacgagatt
      541 gaaaagagtt tccgtaagac gattggaagc caaaaaagca tccacttcgg cggaagcccc
      601 gaatgcggct cccatcaaac ctttggaaat caagccgatt atctgagaga ggatcatcgc
      661 ccccgccagt attgtcgccg atttcataaa tgtttgtttg ggttctgaag tttgttccat
      721 cgcaattgat tctatcatga agggaagaac tctcgaaaag tttattccgg cttgaactct
      781 ctcataactc acatcttaat gaacgagatg actttataat tagcccatca aaaaaagagc
      841 taagaaacgg taaagtctat gaaaaatcct ttagtaattt gttgtattct cggtattctt
      901 atcgggtatt tgttaggcgg agaaaattat ttgacttacg gtatggtcgg cggattattg
      961 gtcggatatc tgttggacga aagaaaccgg tcagcggcta agaaaaaatc gacttccgcg
     1021 acccgacacg aatcaatcga tgtttcggaa ccgacagaca aaactcctca ttccgatgtt
     1081 tcagccgaaa cgaacaaacc attaccggaa cagacacccg attatccgat caagacaatt
     1141 tccgcgaacg gaacatctgt tccgcggatg aacgatgaac aggtcgatga tgtggttgcc
     1201 cgggcacggg aagagatcgc aaaaaataaa gagaaaaata attaatggcg ttgaatcggc
     1261 agcggaattc ggacttcaca taacggttct tcatcatttt cggtgagcgg cgtaaaaacc
     1321 agttcaaaat tggtcatctc cggatgaaca tcaaagatca agaaagtctc aagcccgttt
     1381 ttcccgattt tcgaattcag attgggagtt tcccatctgg aatttgcgta atcggaatta
     1441 ccgcgatcca acattaccgt aacattgcgg ccgtataagg aaccggcaat cgtaaaagat
     1501 tcactcttta aaggcccgat cgtatcattt gtaaggcttc gcatccccaa ataaaccatc
     1561 aaaaaccggc cgatcggaaa atgctcggat aaggtcgcct gaattttcgg gaccatataa
     1621 gtgagtttga cactcatttt ttcaccacaa agaatttctt ggtccagagc gacgggaacc
     1681 aatccggtag gtgaaagata gggtgtgacg gtgacataag cgatttgcaa cttctccgcg
     1741 gtagggcttt tagtcggaac aatttgtgtc gggggttctg tcggaatcgg aaaggtataa
     1801 gtcggaagcg ccgtataggt cggagccggg gtatatgtcg gtaaaggagt aagcgttgaa
     1861 aggtttacgc tactctgtgc cgaaacggat cgaaagatgc cgacgaacaa aatccccgcc
     1921 cagatgatcg tgcgtttcat gttcatggag tacctccgct ttccaacgtt ttataatatt
     1981 ggatcgaaaa ctccggttga ggcatatcca acgaaaatct acaggaaaaa tcactcgaat
     2041 cgtcggcttc gaaaaccaat ttccaatccg ttgcatgagg attgacatca aacccgagcc
     2101 atgtttccat ctccacaccg gggggaatcg cttggctgct taaaaaaggc actccccacc
     2161 ggttactggc actgattcct gcggggccgt aagaaatata ttccgctttt tctcctcgat
     2221 aagtcccgac cgctttaaat tgtttgtgga aaataagcgg aatggttgaa tctgaaatat
     2281 tccagaccat cagattgacc aacaaaaact gcgatttgga tacctcattc cctaccgcgt
     2341 aagcaaaata aggctgtctg tagccgatct ttacggcaaa tcgatcttcg caaaatgcat
     2401 atttactgag gtcgcgagac caaagacccg gattcaccaa aataacattt tgcccggcgg
     2461 tttctcgcaa aatccgaacg gtaggagtta ccgccggaat atttctcgca taaaaagttg
     2521 caatctcttc gggggaatag gttgcatagg gagtctgtgt cggataggga gttccggtcg
     2581 gataaggggt tcttgaactc atcgattgaa tggttttcac caaatcggat accggatcat
     2641 acggtgtcga aacgatttgg gattcagacg gaagcgtaca agccgaaagg ctccctatta
     2701 cccataccaa aaggattatt ttgatcaacc ccgcatattt tcgcatataa aaggattata
     2761 aacgagaaac ctatgctcat ttttatcaaa tcgatgagct cggatttacc gggaagatcg
     2821 aagtataatg gcttttttca ctgatccggg cgatccgatt cagatagcgg tccgcatagt
     2881 gcagcaattc ttgctcggac aaagaagcgg tcaggcagcc tacctctgta accgctttcc
     2941 ctcccataat atttccgtat aaaatcgccg tgcgaaaatc cgcctcatgg tataaaccgt
     3001 aaataaaacc gctgagaaat gcatctcccg ctccggtggc atccgtcgat tcgaattcgt
     3061 cgatatgcgg gatctgccag attccttcct cgtcct
//
LOCUS       DAOSMM010000126         3083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_31707_length_3083_cov_11.071995, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000126 DAOSMM010000000
VERSION     DAOSMM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31707_length_3083_cov_11.071995"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PK898_10170"
     CDS             <1..647
                     /locus_tag="PK898_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20432.1"
                     /translation="QLPLPSSEDLWKPALINTWNGRLANEYCPEYNEEVITINVKDPS
                     VQQWISTTAEGYNWAKSVNLISPDKPIYFTPTDYCDPNNTGPTIQFVNLANGMQITDD
                     VIEIYAVINAPDGIQSFSLDFGKGTDPEEWYVITNGINRQIFNPEKIGEWYLYGMENG
                     LYTLRLYVTSPIGTIAERRIVLSIDIPTKQDAETILVPVYGTEESIPDFFENYP"
     gene            complement(704..1561)
                     /locus_tag="PK898_10175"
     CDS             complement(704..1561)
                     /locus_tag="PK898_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20433.1"
                     /translation="MSGKSYYFFGKVSKFHLLIVIIIVGFAVLLSGCQPKEEEIKSTV
                     ESAINATLKNIPTSTKLPSYTPNPTFTKLPTSTAYPTLTKLPTYTPPATYTVYPTFTK
                     LPTYTPLPTNTIYPTFTKLPTYTPLSTYTKLPTYTPLATYTNLPTYTPLPTGTPRNTY
                     TPLSTYTAYPTYTIYPTSTKLPTYTAQPTYTPIIRIVQVTPTVDSSILKADKTDGFYL
                     IGPEIAPGIWRTEPNKTKCYWKITGVKGEIISNYLGSGGGTIYIPKDAYQIEIQNCGK
                     VTFVQSINN"
     gene            complement(1732..2235)
                     /gene="nrdG"
                     /locus_tag="PK898_10180"
     CDS             complement(1732..2235)
                     /gene="nrdG"
                     /locus_tag="PK898_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOU20434.1"
                     /translation="MCKVRLSGIVPESVCDGPGLRTVLFFQGCCHHCIGCHNPQTWPF
                     DGGEEFTVSDLLRTLPDSPLITGITFSGGDPFYQAEAAALIAKELHNRGKNIWAYTGF
                     KWEELLMESNPSRIKFLLECDVLVDGPFNNKEVKIGLIFRGSLNQRIILVKESFFKNR
                     IILWEPH"
     gene            complement(2246..>3083)
                     /gene="nrdD"
                     /locus_tag="PK898_10185"
     CDS             complement(2246..>3083)
                     /gene="nrdD"
                     /locus_tag="PK898_10185"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOU20435.1"
                     /translation="AAGNIQKFYLLLEEVMTLTSRQLLHRFQIQSHLKVKDMPFLMGQ
                     GLYLGSEKLGSQDEIKEVIRHGTLSIGFIGLAETLIVLTGYHQAQSNDAQKLGLEIIK
                     FMRKMTDHFSEKFDLNFTLLATPAEGLSGRFISIDKEKFGLLPGVTDKDYYTNSFHVP
                     VYYPISSFDKILIEAPYHAFTNAGHISYVELDAPPLYNPEAIEAIIRHMNESGIGYGG
                     INFPIDECPVCGFHGVIEGNCPVCSNTDIRRIRRITGYLSSIDRFNDAKKAELKDRRP
                     HK"
BASE COUNT          911 a          572 c          644 g          956 t
ORIGIN      
        1 accaacttcc cttgccgtca tccgaagacc tttggaaacc ggctttgatt aatacatgga
       61 atggcagatt ggcgaatgaa tattgccctg aatataatga agaggtaata acgataaatg
      121 tcaaagatcc ttctgttcaa caatggattt caactacggc ggaaggttat aactgggcaa
      181 aatccgttaa tttaatcagt cccgataaac cgatttattt tacgccgacg gattattgtg
      241 atccgaacaa taccggccct acgatccaat ttgttaattt agcgaacggg atgcaaatta
      301 ccgatgatgt tatagaaatt tatgccgtta ttaacgctcc cgatggaata caatcattca
      361 gccttgattt tggaaaaggg accgatccgg aagaatggta cgtaatcacg aacggaatta
      421 accgtcagat ttttaatccg gaaaagatcg gcgaatggta tttatatgga atggaaaacg
      481 gactttatac tttaaggcta tatgttacaa gcccaatcgg gacgattgcc gaacgaagaa
      541 ttgtattgtc gatcgatatt cctacaaaac aggatgccga aacgatattg gtacccgttt
      601 atgggacgga agaatcaata cctgatttct ttgaaaatta tccataaaat aaacaggcca
      661 aattctttta gggcctgttt atttatctta tttgtcagga atgttaatta tttattgatt
      721 gcacaaaagt gacctttcca caattttgga tttcaatttg atatgcatct ttgggaatat
      781 atatcgttcc gcctccgctg cctaaataat tggaaataat ctccccttta actccggtta
      841 ttttccaata acattttgtt ttattcggtt cggtcctcca aattcccgga gcaatttccg
      901 gaccgatcaa gtaaaagcca tccgtcttat ccgcttttag aatactactg tcgactgtcg
      961 gtgtaacttg aacaatccga ataatcggag tgtaagtcgg ttgagcggta taggtaggta
     1021 atttagtaga ggtcggatat atcgtataag ttggataggc tgtgtaagtg gaaaggggtg
     1081 tgtaagtgtt tcggggggta ccggtcggca atggagtata tgtcggcaaa tttgtatacg
     1141 tagccaaggg agtataagtt ggaagtttgg tatatgttga cagcggcgta taggtaggta
     1201 atttcgtgaa agtcggatag atcgtattgg tcggtagcgg cgtataggtc ggtaattttg
     1261 taaaagtcgg gtagacggta taagttgccg gcggagtata ggttggtaat tttgtaagcg
     1321 tgggataagc cgtacttgtc ggcaattttg taaaagtcgg atttggagta taggatggta
     1381 attttgtaga agtcggaata ttttttaagg tcgcattaat tgctgattct acagtcgatt
     1441 ttatttcttc ttccttgggt tgacaaccgg aaagtaaaac ggcaaaacca acgatgatga
     1501 tcactatcag cagatggaat tttgagactt ttccaaagaa ataataactt tttccagaca
     1561 ttttgtctcc tttctaaagg ccaatcaact gtaaatcttt ttggactttt aaattttacc
     1621 aaaatatttc atcatgaaag aaaactcttg ttatcctcga tcttaatcag gataaaaggc
     1681 atgaaaacat atgacaaaaa acactgaaat agtggtaatt atattctccg attaatgtgg
     1741 ttcccataaa atgatcctat ttttgaaaaa actttccttt accaggataa ttctttggtt
     1801 caaagatccg cgaaaaatca gccctatttt gacttcttta ttattaaatg ggccgtctac
     1861 cagtacatca cattcgagaa gaaattttat gcgggacgga tttgattcca ttaataattc
     1921 ttcccattta aaacctgtat atgcccaaat gttttttccg cgattatgta actcttttgc
     1981 gatcagcgcg gcagcctcgg cttgataaaa cggatcacct ccactgaaag taattcctgt
     2041 tattaatggg gaatcaggca gcgttctgag caaatccgaa accgtgaatt cttctcctcc
     2101 gtcgaaaggc caggtttgag gattgtggca gccgatgcaa tggtggcagc atccttggaa
     2161 aaacagtaca gtacgaagtc cgggtccatc gcaaacactt tccgggacaa ttcccgataa
     2221 tcgaacttta cacataatat tattcctatt tatgcggacg acgatctttt aattctgctt
     2281 tcttcgcatc attaaatcga tcgattgaag agagataacc tgtgatacgg cggatccgcc
     2341 ggatatcggt attgctgcat accgggcagt ttccttcgat tacgccatga aagccacaaa
     2401 cgggacattc atcaatcgga aaattaattc ctccatagcc gattccgctt tcattcatat
     2461 gtcgaattat tgcttcgatc gcttccggat tataaagcgg cggtgcgtct aattccacat
     2521 aactgatatg gccggcattt gtgaaagcat gatagggtgc ttcaattaaa attttatcga
     2581 atgatgaaat cggataataa accggaacat gaaagctatt tgtataataa tctttgtcgg
     2641 ttacgcccgg caataaaccg aatttttctt tatcgatcga gataaaacgt cctgaaagtc
     2701 cttcggcagg cgttgccaat agtgtgaaat tcagatcaaa cttttccgaa aaatgatccg
     2761 tcatttttct cataaacttt attatttcca gtcctaattt ttgagcatcg tttgattgag
     2821 cttgatgata tccggttaat acaataagtg tttccgctaa tccgataaat ccaatggaaa
     2881 gtgtcccgtg gcggatcact tctttgatct catcttggct gccgagtttt tccgatccca
     2941 aatatagacc ctgtcccatg agaaaaggca tatcttttac tttaaggtgt gattgaattt
     3001 gaaatcgatg cagaagttga cgagaggtaa gtgtcataac ttcctcaaga agaagataaa
     3061 atttttgaat atttcctgcg gca
//
LOCUS       DAOSMM010000127         3026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_32499_length_3026_cov_16.695725, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000127 DAOSMM010000000
VERSION     DAOSMM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32499_length_3026_cov_16.695725"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PK898_10190"
     CDS             complement(<1..1186)
                     /locus_tag="PK898_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20436.1"
                     /translation="MKKNILYVVLFLLIFAVLPVNAAGDTLVIGVATDMTGMDPHQQT
                     AFTSMKVLEMMYGQLVEVNENMEVVPSMAESWEWSEDGMHLTMHLVPEMTFHDGSPVT
                     SADVKYSFERILNEETAAAARSKFTAISSIDTPDDLTVVFNFGEKNVAILVDMASTNA
                     AILSKAFMENGGDLSTTEMGSGPYMMKKWEPDNKIEMVAYEKYFMKGLPKIKNLEFRI
                     IPSEDSILAGIRTGEIDFAVINEPATAMIASKDKNVVVMEAPALSYHVLQLNPSREVF
                     KNVKVRQAIACAIDRQEVLDVASLGEGEVTPPAVQPFYKANLDDLFCYKKDLEKSKAL
                     LAEAGNPQVEFTIMAAADEPPTAVAEAQNIQDQLSEIGIKVNIEVLELGVYVDRWLAG
                     DFD"
     gene            1555..2457
                     /locus_tag="PK898_10195"
     CDS             1555..2457
                     /locus_tag="PK898_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU20437.1"
                     /translation="MNFKKITDSYHFYAIITILCWAVAPVLTRLCHRHFSAMPIGFLR
                     YLISSVLIILLLIIKKTKFPSIKDLPWLIASAATGFSLYMVMFNYGTQYVESATTSVM
                     MATSPLITALLSFFLFKETLSKNQWLAILIEFIGIIILTVVGNGFSLNIGTLWLFGAS
                     FLLSVYNLIQRILTKKYSGFQVTAFSILIGTLMLSFSAPQSFRELKTAPPNQIFYLLI
                     LGIFTSTIAYIAWSTAFQKAEKTSQVSNYMFFNPLLSGILAFFIAGEIPDSTTYIGGI
                     LILLGMWLFNHGQAIQPKAKIHSH"
     gene            complement(2532..>3026)
                     /locus_tag="PK898_10200"
     CDS             complement(2532..>3026)
                     /locus_tag="PK898_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20438.1"
                     /translation="WLFVFLTYILLYRTAFGLRMRGVGEHSEASSTLGVNVHKTKYIA
                     ILLSGVLCGLAGAQLSLGNVTMFVEDMSSGRGWIAIVANLLGQSLPLGVFLSSLLFGF
                     VTSLSFRFQGLGWASELTEMLPYIITIISLVLVYIFSDKKMKRPSKEILAYKNEQMNH
                     RTDG"
BASE COUNT          891 a          706 c          525 g          904 t
ORIGIN      
        1 catcaaaatc accggctaac cagcgatcca cataaacgcc caattcaaga acctcgatat
       61 ttacttttat accgatttcg gacaattgat cctgaatatt ttgtgcttcc gcaacagcgg
      121 tcggcggttc atccgcggca gccataatcg taaattcaac ttgggggttt cccgcttccg
      181 ccaacaaagc ttttgatttt tccaaatctt ttttatagca aaacaggtca tccaaattcg
      241 ctttataaaa aggctgtacg gcgggcggag ttacttcccc ttcacccaaa gaggcgacat
      301 ccaaaacttc ctgccgatca atggcacagg caattgcctg ccgaactttc acattcttga
      361 aaacttctct gctcggattt aactgtaata catggtaaga aagagccgga gcttccatca
      421 cgacaacatt tttatctttg gaagcgatca tggcagtggc aggctcatta atcaccgcga
      481 aatcaatctc acctgtacga attccggcca gaatcgaatc ttcggaagga ataattctga
      541 attccagatt tttaatcttc ggcaaccctt tcatgaaata tttttcatag gcaaccattt
      601 cgattttatt atccggttcc cattttttca tcatatacgg accggatccc atttcggtcg
      661 ttgacagatc gccgccgttt tccataaaag ccttcgatag aatcgccgca ttggtcgaag
      721 ccatgtccac taaaatggcg acatttttct cgccgaagtt aaaaacaacc gttagatcat
      781 ccggagtatc gatggaagaa atagccgtaa attttgaacg ggctgcggca gcggtctctt
      841 cgttcagaat gcgttcgaaa gaatatttca catccgcaga agttaccggc gaaccgtcat
      901 gaaatgtcat ctccggaacc agatgcatcg tcagatgcat cccgtcctca ctccactccc
      961 acgattccgc catactcggc acaacttcca tattttcatt tacttcaacc agttggccat
     1021 acatcatttc aagaactttc attgaagtga aagccgtttg ttgatgagga tccataccgg
     1081 tcatatccgt ggctacacca ataaccagtg tatcacccgc ggcattaacc ggcaaaaccg
     1141 caaaaatcaa caaaaacagg acaacataaa gaatattttt tttcatcttt tctcctttta
     1201 attcttttca atatagaacg aaaccgtact gataaaacct tatctgaaaa ttttcttttt
     1261 gtcaaatgaa gccgaccggc tcgccggcga ccctgttcga ttctcctgcg ttctttttca
     1321 gtttgcgggg ggagagatta tccaatcaag ttaatttctc cgtaatattc ttccattata
     1381 atttgttgta ctttttatat gaacagttcg gttttttatc gaaaacttac cgggtcggaa
     1441 gaaatgccaa attaactcaa aacaccgaaa caaaaccgac ataattcttc cgtaatttag
     1501 gttttcagga tcccgatcca aacatttaat aagatactta ctctgggaaa taggatgaac
     1561 ttcaaaaaaa tcacagacag ttatcatttc tatgccatca taacgatcct atgctgggca
     1621 gtcgcgccgg tcttaacccg cttatgccac cggcatttct cggcaatgcc gatcggattt
     1681 cttcgttatc tgatatcctc cgttttgatt attcttctgt tgataatcaa aaagacaaaa
     1741 tttccttcga taaaagatct tccctggctg atcgcttctg ccgctacagg cttttctcta
     1801 tatatggtta tgtttaatta cggcacacag tatgtggaat cagccaccac cagtgtcatg
     1861 atggcaactt ctccgttgat cacagctctt ttatcctttt ttctttttaa ggaaaccttg
     1921 tctaaaaacc aatggctggc gattctgatc gaatttatcg gaattattat attgactgtc
     1981 gtggggaacg gtttctcgtt aaatatcgga acgctttggc tatttggagc ttctttttta
     2041 ttaagtgtct acaacttaat tcaacgaatt ctgacaaaaa aatacagcgg attccaggta
     2101 accgctttca gcatcctgat cgggacactt atgctatcat tttccgcacc gcaaagtttc
     2161 cgtgaattga aaaccgctcc tccaaatcag attttttatc tgttgatatt gggaatcttt
     2221 accagcacca ttgcttatat cgcatggtca accgcttttc aaaaagccga aaaaacttcg
     2281 caggtcagta attatatgtt cttcaacccg ctcttatccg gtatattggc tttctttatc
     2341 gcaggtgaaa tcccggacag cactacatat atcggaggta tattgatcct attggggatg
     2401 tggttattca atcacggaca agcgatacag ccaaaagcaa aaatacactc tcactgataa
     2461 aaagaataca gatatttttt cccatttata tatgtttttt agataatcaa taggcaatag
     2521 acgaattatt tttatccgtc ggtccgatga ttcatctgct catttttata agccagaatt
     2581 tcttttgaag gccgtttcat ttttttatct gaaaaaatat aaaccaaaac caaactgata
     2641 attgtaataa tataaggaag catttcggtc aattcagatg cccaccccaa accttggaag
     2701 cgaaaactta atgacgtcac aaaaccaaat aacagactgg aaagaaaaac tcctaagggt
     2761 agagattgtc caagcaaatt tgcgacaatt gcgatccagc cgcgtccgct ggacatatct
     2821 tcaacgaaca tagtaacatt tcccaatgat aattgtgctc ccgccaatcc gcacaatact
     2881 cccgataaaa gaattgcaat atattttgtt ttatgaacat taacacccaa tgttgatgaa
     2941 gcttccgaat gttcgccgac gcctctcatt ctgagtccga atgcagttcg atataacaat
     3001 atataagtta gaaacacaaa aagcca
//
LOCUS       DAOSMM010000128         2983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_33090_length_2983_cov_15.084016, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000128 DAOSMM010000000
VERSION     DAOSMM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33090_length_2983_cov_15.084016"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /gene="pyrH"
                     /locus_tag="PK898_10205"
     CDS             complement(<1..712)
                     /gene="pyrH"
                     /locus_tag="PK898_10205"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOU20439.1"
                     /translation="MTDLKYQRILLKLSGEALSPKEENGIDPQSANKIAQQVKEVFDM
                     GADLAIVIGGGNIWRGKIGIEVGMDQATADYMGMLATVMNSLVLMDALEHIGVTTRVQ
                     TAIEMRSIAEPYIRRRAIRHIEKKRVVIFAGGTGNPYFTTDTAAALRAVEINANLLIK
                     ATKVDGIYDSDPKINPNAVKFDTIRYIDVLSRQLQVMDATAISLCMENKMPLLVLNLW
                     EEGALSKAVRGEPIGTLVT"
     gene            complement(885..1505)
                     /locus_tag="PK898_10210"
     CDS             complement(885..1505)
                     /locus_tag="PK898_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOU20440.1"
                     /translation="MGTVSTDLIKQLRESTGAGILECRKALENANGDLKAAMESLREK
                     GLATALKRSSRAASEGVIEVYTHGGGRVGVMLELNCETDFVGRSESFRYLAHELALQI
                     AASSPLYISEEDIPQSVLDNEAKIAEAKAREEGKSEAIIPKIVEGYLKKYKDEVVLLN
                     QIYIRDESIKVQDLINQNIAALGENIIVRRFARYALGESSDTEEAE"
     gene            complement(1510..2406)
                     /gene="rpsB"
                     /locus_tag="PK898_10215"
     CDS             complement(1510..2406)
                     /gene="rpsB"
                     /locus_tag="PK898_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOU20441.1"
                     /translation="MTSVISMKALLESGVHFGHRTNKWHPAMRPYIFTERNGIHIIDL
                     QQTAKSINTVYPLVRDTVQSGKEILFVGTKRQAQETIRDEAIRCGMPYVTERWLGGTL
                     TNWTTIYQRINELEKLEKMKETGEINKLTKKEGLMIEREIARLELRLSGLRNMKTLPG
                     LLFVIDVMREATAIHEANLKNIPVIAMVDTNCDPTNVDYVIPSNDDAIRAIKLIVSKI
                     ADAVIEGKALRGEGNFDEGSFSDDSKPNNAKHLYNDTGDMEDEDLFGASTLAKLGNMK
                     AAKQDAGEDSNGGEYDESEEEE"
BASE COUNT          783 a          656 c          570 g          974 t
ORIGIN      
        1 gagtaacgag tgtcccgatc ggttcccctc ttaccgcttt gctgagagcc ccttcttccc
       61 aaagatttag aaccaataac ggcattttat tttccataca aagagaaatg gcagtcgcat
      121 ccattacctg taactgccgc gataaaacat caatatatcg aatggtatcg aatttgacag
      181 catttggatt tattttcgga tcggaatcat aaattccatc cactttcgtg gctttgatca
      241 agagattggc gttgatctca acagccctca aagctgcggc tgtatctgtt gtaaaataag
      301 gattcccggt gccgcccgca aaaatgacta ctcttttttt ctcaatgtgt cggattgctc
      361 gtcttcggat atacggctcg gcgatcgaac gcatttcgat ggctgtttgg acgcgggttg
      421 taacaccgat atgttccaat gcatccatta agacaagtga attcatgacg gtagcaagca
      481 ttcccatata atcagcggtt gcttgatcca tcccgacttc gattccgatt tttccacgcc
      541 agatattccc tcctccgata acaattgcca gatccgcccc catgtcgaaa acttctttga
      601 cttgttgagc aattttattg gctgattggg ggtcgatccc attttcttcc ttgggggaaa
      661 gcgcttcacc gctgagtttt agtaatatcc gttggtattt taagtctgtc atttgaggcc
      721 tcttcgctat ttttctgaat ggttcgcttt tctttcgcaa tctggaataa ttataaggga
      781 ttaagaaaac cttcttgatt ttgaaaattt tcgtaagcgt ataaaaaaac cggtttccct
      841 atgaaaaccg gtttttaaag tttgtgttga taaggtcgga atgattattc tgcttcttct
      901 gtatcggatg attcacctaa cgcataacgc gcaaaccgtc tgacaatgat attttcacct
      961 aaagcggcta tattctgatt gattaaatct tgaaccttga tggattcatc ccgaatgtaa
     1021 atttgattta ataggacaac ttcgtcttta tattttttca agtaaccttc cacaatcttc
     1081 ggaataatcg cttcactttt tccttcttca cgggctttag cttcggctat ttttgcttca
     1141 ttatccaaaa cactttgcgg gatatcctcc tcactgatat aaagcggaga agatgcggca
     1201 atctgtaagg ctaattcatg agccaagtaa cgaaacgatt ccgaacggcc gacaaaatca
     1261 gtctcacaat ttaactccaa catgacaccg actcgaccgc cgccgtgagt ataaacttca
     1321 atgacacctt ctgaagcggc tcgactggat cgttttaaag ccgttgcaag ccctttttct
     1381 cgtaaggatt ccatcgctgc tttcagatcg ccgtttgcgt tttccagtgc ttttcgacat
     1441 tcaaggatgc ccgcaccggt actttcacgt aattgtttaa taagatctgt actgactgta
     1501 cccatttaat tattcctctt cttcgctttc atcatattcg ccgccattcg agtcttcgcc
     1561 ggcatcctgt ttagcggctt tcatgttgcc taattttgcc aatgtcgatg ctccgaataa
     1621 atcttcatct tccatgtctc cggtatcgtt atacaagtgt ttggcattat tgggcttaga
     1681 atcatctgag aaggatcctt catcgaaatt accttcaccg cgtaatgctt ttccttcaat
     1741 caccgcgtcg gcgattttgc tgacaatcaa cttgattgcc cgaatggcat catcattgga
     1801 ggggatgaca tagtcaacat tcgtcggatc gcaattggta tcgaccattg cgatgacggg
     1861 aatattctta agattggctt catgaattgc tgttgcctca cgcatcacat caatgacgaa
     1921 aagcaacccc ggtaaggttt tcatgtttcg taaaccgctg agtcgaagtt ccaaacgagc
     1981 aatttcacgc tcgatcatca agccttcttt tttagtgagc ttgttaatct ccccggtttc
     2041 tttcattttt tccagctttt caagctcatt gatacgctgg taaattgttg tccagttggt
     2101 taatgtgccg cccagccatc gttcggttac ataaggcatt ccgcaacgaa tcgcttcatc
     2161 ccggatggtc tcctgtgcct gacgttttgt cccgacaaaa aggatctctt ttccggattg
     2221 tacggtgtcc cgtaccaatg gataaaccgt gttgatactt tttgcggtct gctgtaaatc
     2281 aataatatgg atcccgtttc gctcggtaaa aatataaggc cgcatggcgg gatgccattt
     2341 gtttgttcga tgtccaaaat ggacaccact ttccaataat gctttcatgg aaataacact
     2401 ggtcataata aaaactcctt ttgcgtttta gcctctgctt tcgtcaattt ctttttagtc
     2461 acaaccttat cgggttgcac gcagcaaaaa gatcagaaag catgtgtttt atattgccgc
     2521 atgattttat catggaatct aaattaaata aaacaaaact gatggaaaaa tgctttttcc
     2581 atagacttag agatcatata aatcgatttt tccatcagta gtcggagctt tttattccct
     2641 atgattgtaa aatccggttg attttgaaaa gatcttctga ccctattatt gcaaaaaatg
     2701 tcattagaat ccggacatat cagactaagt atatggcaac ttcaatattt ctcaaattga
     2761 aatttctccc attcgatgtt tttaaaaagg atatgcgatc tgatccggct tttgggatct
     2821 gaaaatttta tataatccaa ctttaatttt tttaggctac aattttgttt ataatttgaa
     2881 aagagagaag cattttaagg ataaaatcaa ttgaatgccg gtgagacgga agggaaaaag
     2941 acatgttgat cagatttgca tatttaatgg ctccgaggaa tta
//
LOCUS       DAOSMM010000129         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_33167_length_2977_cov_15.388433, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000129 DAOSMM010000000
VERSION     DAOSMM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33167_length_2977_cov_15.388433"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PK898_10220"
     CDS             <1..898
                     /locus_tag="PK898_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20442.1"
                     /translation="SIAIFLIMHMIPGDTISALIGTQYQLTEAQAESLRAYYGLDKSL
                     PDQYISWLLNALQGNFGLSVRTGQPVMKEILARFPLTFEITMLTIILGTILGILFGII
                     AAVYRGSVMDMIVRIFGMIGLATPSFWLGTMIIYFLSVHFGIMLNAGNYVPFSQDPIQ
                     NLKQVISPVICMGFSFSASVMRTTRSSLLEEINKEYALTSRAKGNIEFVTIFKHALRN
                     ALIPVITLVGYQIGYLFGGVVIIEQVFALPGLGRFLLNGISQRDYALVQGTILFIAAN
                     FVLVNLIADVAYGFADPRIRYD"
     gene            901..1797
                     /locus_tag="PK898_10225"
     CDS             901..1797
                     /locus_tag="PK898_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20443.1"
                     /translation="MNRELISEEAKSQQRKRNFQYSAVGRLFRNPSGVIGLILVIIFI
                     VIAVFSDYLMPYDPIKNYPKERLQAPSSEHLAGTDEMGRDCMSRLMKGATNSLSIAFV
                     SVAFAGIIGTFLGSLCGYYGGWFDSLIMRLMDLIFSFPAMLLALAISAALGAGFLNTV
                     MAISIVYLPIFSRTARGSVLQIREMEYIEAARCVGAKNMRIIFRHILPNALAPLIVQV
                     SLGLSWAILTESSLSYLGLGTQPPNPSWGSMLSQSRVMMELAPWLMIGPGAAIMLAVL
                     GFNLLGDAIRDLTDPRLRSRGL"
     gene            1805..2272
                     /locus_tag="PK898_10230"
     CDS             1805..2272
                     /locus_tag="PK898_10230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU20444.1"
                     /translation="MSSVSVSRIREEEIPACAQIMAGNSLWQHYDVTYESALARFQNG
                     YAEQATIFIAHEGEEVCGFCWVAEKGAFNRSAYIMLIGVDPLRQSRGIGKILLEETEK
                     YLATKSVDVFLTVSDFNIGAQKFYSGMGYKTVGEIPDYVKPGITEIIMRKVLR"
     gene            2310..>2977
                     /locus_tag="PK898_10235"
     CDS             2310..>2977
                     /locus_tag="PK898_10235"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOU20445.1"
                     /translation="MIILGMMSGTSVDGIDAVLVDIVGAPPRFEWKIIGHHQMPFPEA
                     IRREIFDCFDPKKGTVDRICSLNFKLGRVYGEAALETIRQLGYSPEQVRFIGCHGQTV
                     WHIPANEPDASTLQLGDPSVIAEITGLPVVSHFRNADMAAGGHGAPVVSFMDVMLLSD
                     SKKVRAAQNIGGIGNVTFLPPKNREYSDIEPIAFDTGPGNMLIDCAVEQITGGEKHFD
                     EDGK"
BASE COUNT          733 a          574 c          786 g          884 t
ORIGIN      
        1 atctatcgcc atttttttga tcatgcatat gattccgggt gatacgatca gtgcgctgat
       61 cggaacacaa taccaactga ccgaagcaca ggctgaatcg cttcgggcgt attacggatt
      121 ggataaatca ttgccggatc aatatatcag ctggctgttg aatgcgttgc aaggtaattt
      181 tggattatcg gttagaaccg gtcagccggt tatgaaagaa attttagccc gattcccatt
      241 gactttcgag ataacgatgt tgacgatcat tctggggacg attttgggga ttctttttgg
      301 gattatcgcg gctgtgtatc ggggttccgt gatggatatg attgttcgga ttttcggaat
      361 gatcgggttg gctactccga gtttttggtt aggaacgatg atcatttatt tcctttcggt
      421 tcatttcgga attatgctaa atgcgggaaa ttatgttccg ttcagtcagg atccgataca
      481 aaacctaaaa caggtgatct ctcccgttat ctgtatggga ttttcattca gtgcttccgt
      541 aatgcgtaca acgcgttctt ccctgctgga agaaatcaat aaggaatatg cactcacttc
      601 cagagcgaag ggtaatatag agttcgtgac catctttaag catgcgctcc gaaatgcgct
      661 tatcccggtg attaccttag tcggctatca gatcggatat cttttcggcg gagtagtgat
      721 tatcgaacaa gtatttgcgc tgccggggtt gggaagattt ctgttgaacg gaatttccca
      781 gcgtgattat gctctggtac aaggaacgat tctgtttatc gccgcaaatt ttgttctggt
      841 gaatctgatt gcggacgtcg cctatggctt tgccgatccg cggataaggt atgactgaaa
      901 atgaatcggg aacttatctc tgaagaagcg aagtctcaac aaagaaaaag gaattttcaa
      961 tattccgctg tgggccgtct ttttcggaat ccaagcggag ttatcggatt gatcttagtg
     1021 attattttca ttgtgatcgc cgttttctcg gattatttga tgccgtatga cccgataaaa
     1081 aattatccga aagagcggtt acaagcgccc tcttccgaac acttggccgg tacagatgaa
     1141 atgggccgag attgtatgag tcgactgatg aaaggcgcta cgaattcttt atcgatcgct
     1201 tttgtctctg tcgccttcgc gggaattatc ggcacttttt tggggtcgct ttgcggatat
     1261 tatggcggct ggtttgacag cttaatcatg cgtctgatgg atttaatttt ttcgtttccg
     1321 gcgatgctgt tggcgttagc catttccgct gcgttgggag cgggcttttt gaatactgtg
     1381 atggcgattt ccattgtcta tctgccgatt ttctctcgaa ccgcgcgcgg ttcggttttg
     1441 cagattcgtg aaatggaata tattgaagct gcccgatgtg tcggtgcaaa aaatatgcgg
     1501 attatttttc ggcatatttt accgaatgcg ttagctcctt tgattgttca ggtcagcctg
     1561 ggattatctt gggcaatctt aaccgaatct tctctgagct atttgggatt gggaacgcaa
     1621 ccccccaacc cgtcctgggg ttccatgttg agtcaatcaa gagttatgat ggaattggct
     1681 ccatggttga tgatcggtcc cggtgcggct attatgctgg ctgtattggg gtttaatttg
     1741 ctcggtgatg ccattcgtga tctgacggat ccgcgcctgc gttcaagggg gttatgaaat
     1801 aaagatgtct tctgtcagcg tttctcgaat tcgagaggaa gaaataccgg cttgtgcaca
     1861 aattatggcc ggaaattcgc tttggcaaca ctatgatgtg acttatgaaa gtgccttggc
     1921 aagatttcaa aacggatatg cggaacaggc gacgattttt attgctcatg aaggcgaaga
     1981 agtatgtggt ttttgctggg tggcggagaa aggtgctttc aatcgttcgg cttatattat
     2041 gttgattggg gttgatcccc tgagacaaag tcggggcata ggcaaaatac ttctcgaaga
     2101 aacagaaaaa taccttgcaa caaagagtgt cgatgttttc ttaaccgttt cggattttaa
     2161 tataggcgca caaaagtttt attcaggcat ggggtataaa acggtgggag aaatcccgga
     2221 ttatgtcaag cccggaatca ccgaaataat aatgcggaaa gttttacgat aagtatcaac
     2281 cttctgtgat ggaagtaaag gaaaagaaaa tgattattct cggaatgatg tccggaacct
     2341 ctgtagacgg aatcgatgct gtactggtcg atatagtagg cgcacctcct cgtttcgaat
     2401 ggaaaattat cggtcatcat cagatgcctt ttccagaagc gatccgcagg gaaatctttg
     2461 attgttttga tccgaaaaaa gggacagttg atcgaatctg cagcctaaat tttaaattgg
     2521 gacgtgttta cggcgaagcg gcgttggaaa ctatccggca attgggatat tcaccggaac
     2581 aggttcgatt catcggctgt cacggtcaga cggtatggca tataccggcg aacgaaccgg
     2641 atgcatccac tttgcagctg ggtgatccat cggttattgc tgaaattacg gggttgcccg
     2701 ttgtgagtca ttttcggaat gccgatatgg cagccggagg gcacggtgcc ccggtggtat
     2761 cctttatgga tgtgatgctg ttatccgatt cgaaaaaagt gcgggcggct cagaatatcg
     2821 ggggaatcgg gaatgttacc tttttgccgc cgaagaatcg agaatattcg gatatcgagc
     2881 cgatagcgtt tgataccggt cccggaaata tgctcattga ttgtgcggtg gaacagatta
     2941 cgggagggga aaaacacttt gacgaagacg gcaaaat
//
LOCUS       DAOSMM010000130         2948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_33603_length_2948_cov_20.519184, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000130 DAOSMM010000000
VERSION     DAOSMM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33603_length_2948_cov_20.519184"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..586
                     /locus_tag="PK898_10240"
     CDS             152..586
                     /locus_tag="PK898_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ohr family peroxiredoxin"
                     /protein_id="HOU20446.1"
                     /translation="MGTITKYEAAVTNTGGRDGIVFSPDHSFSMVIEPPDKSGEKHAG
                     ASNPEQLFAAGYSACFNNALQSSLRRDKVEFESSTVTVFVALLYDPDEKGHRIGARIE
                     ASVKGINLDEAKKYVAIAHTRCPYSKAIQGNVDVKIDVVEAE"
     gene            complement(662..1156)
                     /locus_tag="PK898_10245"
     CDS             complement(662..1156)
                     /locus_tag="PK898_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU20447.1"
                     /translation="MTKKWDGAEFFRLMAANEQAVGDWYRTLANDAKFGGKFFEKMAK
                     DEDRHYQIYTALLKKYEGTQALTVEVSEDHEAYLNALMKDHIESDADNLKAKAAKAST
                     KDDIYAIAEQVERDSVLFVEELIELYPDLKEEDFKVVLQEEKNHLAMVLTRRMDSQLN
                     TLRL"
     gene            complement(1336..1887)
                     /locus_tag="PK898_10250"
     CDS             complement(1336..1887)
                     /locus_tag="PK898_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU20448.1"
                     /translation="MNDLEFIKSKKNQNLNGGNMGNLFNGAEFFEMMAANEKAVGDLY
                     RFLAKDAKFGGKFFENMAADEDRHYGIYTSLLKKYQGKKDLMVELAEDRKSYMDLLIK
                     NNYLSDPEKLKERAANAKSKEDIFDIAERIERDSVMFTQEMIDLFPDLDPADFKVVLQ
                     EEKKHLAMVLDKRMDSQLKSLRL"
     gene            1969..2892
                     /locus_tag="PK898_10255"
     CDS             1969..2892
                     /locus_tag="PK898_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU20449.1"
                     /translation="MQNKEAVQSPETNQNKDHPQSQAGLLAIHSAVLLFGLSGVIGKS
                     LDLPAVIITFARVFISAIFLFLILISKKEPVRIRSLRNFSPFLAAGLIMAIHWTTFLQ
                     SIRTSTVAIGTLTFSTFPLFVTFLEPYFFREKLKAGAVLEALGMLLGVFILVPEFEIG
                     NRMTQGILWGMAGSISYAFLSLINRRLAEKYSGEQISFYEQFVATILLLPSLWLIPFK
                     LSGKDMAGLLILGIVCTGIGQALFITGLKNVRVQTAGIISGLESVYGIIFAFLFLGEA
                     PGIREICGGLIILGISTCATLTSARKNAPNR"
BASE COUNT          798 a          696 c          587 g          867 t
ORIGIN      
        1 tttcgccgtc ccctaccatc ctctcccaca gggcaaaaga aaatgcgccc atacgtatca
       61 tgtcaaacgg ccaaatatcc gcgggggtag tttccgaaaa taatgataaa tttaagaaaa
      121 gcgctaaacc gcgaaccgaa aggaaaaaac gatgggcaca attacaaaat atgaagcagc
      181 agttacgaat accggaggtc gggacgggat cgttttctcc ccggaccatt ctttttcaat
      241 ggtgatcgaa ccgccggata aatccggcga aaaacacgcc ggagcaagca acccggaaca
      301 gttgttcgca gccgggtaca gcgcctgctt taataatgcg ctgcaatcat ctttacgccg
      361 ggataaagtg gagtttgagt ccagtaccgt cacggtattt gtcgcgctgt tatatgatcc
      421 cgatgaaaag ggacaccgga tcggagcccg cattgaggca tccgtcaaag gaatcaacct
      481 ggatgaagct aagaaatacg tggccatcgc tcataccaga tgcccatatt ccaaagcgat
      541 tcagggaaat gtagatgtaa aaatcgatgt ggtcgaagcg gaataataat taatcgaaaa
      601 acaggttgtt ccggatctta attcgggtac agcctgtttt tcgaattttt tcgatacgaa
      661 attacaatct gagcgtattc aactgactat ccattcggcg agtaaggacc attgccaagt
      721 ggtttttctc ttcctgaagc acgaccttaa aatcttcttc tttcaaatcc ggataaagct
      781 caatcaattc ctcgacgaac agtaccgaat cgcgttcgac ttgttccgca attgcgtaga
      841 tatcatcttt cgtggaggct ttggcagcct ttgctttcag attatcggca tcgctttcga
      901 tatggtcttt catcagcgca ttcagataag cctcatggtc ttccgacact tcaactgtca
      961 atgcctgagt gccttcatac tttttcaaaa gagccgtata gatctgatag tgacgatctt
     1021 catccttcgc cattttttca aagaatttcc cgccgaattt tgcatcattc gccaatgttc
     1081 gataccagtc tcccacagct tgttcattgg ctgccatcag acggaaaaac tccgcaccat
     1141 cccatttttt agtcatattt ctcctccaat cgatatttcc tttgcgattt tttagcgaca
     1201 aaaaaacaaa ccttttttta ttattacggt caatcattaa atagtcaaga tttaccgact
     1261 tttcataaaa caaaaagccg cctgatcaac ccaaacggaa tgaacagaca gctttccaag
     1321 caaactcaaa caaatttata atcgcaggct cttcagttga ctatccattc ttttatccaa
     1381 caccatcgcc aaatgctttt tctcttcctg cagaacgact ttgaaatcag ccggatccaa
     1441 atcagggaac agatcaatca tttcctgagt aaacatcacc gaatcccttt cgattcgttc
     1501 ggcgatatca aagatatctt ccttactctt ggcatttgcc gctctttcct taagtttttc
     1561 gggatcagaa agataattat tttttattaa aagatccata tagctctttc gatcttccgc
     1621 caattcaacc attaaatctt ttttcccttg atatttctta agtaaggaag tatatatccc
     1681 ataatggcgg tcttcatctg ccgccatgtt ttcaaagaat tttccgccga actttgcatc
     1741 tttggccaga aatcggtaca gatcgccgac agctttttcg ttagctgcca tcatttcaaa
     1801 aaactcagcg ccattaaaaa ggttgcccat atttcctccg ttcaaatttt gattcttttt
     1861 tgatttgatg aattcaagat cattcatcag actttcgata taaatttatt ataatggttg
     1921 taatagtatc cttcaatagg gaagatttcc gcaagaaaag tcttcacaat gcaaaacaaa
     1981 gaagccgttc aatcgccgga aacaaatcaa aacaaagatc acccacagag tcaagcggga
     2041 ttgttggcga ttcactcggc tgtactctta ttcgggcttt ccggtgtcat cggaaaatcg
     2101 cttgacttgc cggccgttat cattactttt gcccgtgttt tcatctcggc gatttttttg
     2161 tttcttattt taatttcgaa gaaagaaccc gttcgaatac ggtctttgag aaacttttcc
     2221 ccctttttgg cagccggttt gattatggcc atccattgga cgacttttct gcaatccatt
     2281 cgcacatcca cggtagccat cgggacactc actttttcca cttttccgct ttttgtcaca
     2341 tttcttgaac cgtatttctt ccgagaaaag ctgaaagccg gcgccgttct tgaagcgctc
     2401 ggaatgctgc tgggcgtatt tatcctggtc ccggaatttg agatcggcaa ccggatgacg
     2461 cagggaatcc tgtggggcat ggcgggcagc atcagttatg cgttcctttc gttgatcaac
     2521 cgccggctcg cagaaaaata ttccggcgaa cagatcagtt tttatgaaca attcgtcgcg
     2581 acaattctct tgctaccgtc gttatggttg attcctttca aattatccgg aaaagatatg
     2641 gccggtcttc ttatcttagg gattgtctgc acgggtatcg gacaagcctt gtttattacg
     2701 gggttaaaaa acgtccgcgt acagacggcg ggaattatct ccggtttgga atccgtttac
     2761 ggaatcattt ttgctttcct gtttttgggg gaagcgccgg gaatccgtga gatttgcggg
     2821 ggattgatca ttctgggaat ttcgacctgc gcaacattaa cgtcggctcg aaaaaatgcc
     2881 ccgaatcgat aaaaaaacat cctctgagtg atcagaggat gtggttgtca agctcaaaca
     2941 aatcaaat
//
LOCUS       DAOSMM010000131         2854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_35071_length_2854_cov_8.441586, whole genome shotgun sequence.
ACCESSION   DAOSMM010000131 DAOSMM010000000
VERSION     DAOSMM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35071_length_2854_cov_8.441586"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK898_10260"
     CDS             complement(<1..222)
                     /locus_tag="PK898_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20450.1"
                     /translation="MNSKKSIFRLVTAVLFVCVLAGTFAVEAATEFTPVSEGCKDAVN
                     DGITFTTAASISGSAVEVWRDRIQVTVTDP"
     gene            complement(470..2668)
                     /locus_tag="PK898_10265"
     CDS             complement(470..2668)
                     /locus_tag="PK898_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20451.1"
                     /translation="MKKRTIRILFLLIFIAVVIGLMSGSIRRQALPYYIALLILITGM
                     DFYLSGKSIERAIAWVAIWAEAGWEILFLVKDSVKWFSLRAAALEKFSFFFLIFFFFG
                     VSLHLWLLFGKPDITRRSNASLSEDKLIAFFQRRKVEILICILTALSLRYLMHSGYYW
                     DDAVNSTAYLFEKQDQLPLIQNLVNFMRKYLELGRINLLSCYYYFLFYIENVKLYKLI
                     IILSVCINQIIFGMLVRYAGGSDRLGQAAMLSIPILIQFRGYQDPVTGFYSLMQVILL
                     ELMLTAYFLLRYLRENRKKFLWFGLIPFFCGLMTYEVCFPFILMIPVIAWLETRDRKK
                     TVQVCIPYGILFAGTLTTALIVRNRLIQGTPYSGIAFHFDVLKILKTYGLQLLAGLPF
                     SFYFFTRSIPIMGNVYPAAEVFSDRAWDILCHTEAADWLLALGIVSLFSLILKKGKEN
                     QKDTSTRLLAGIGLSFWLLPGMTIALSDRYQGQIVPGLGYLPVYLEYFGIGILALCLV
                     REGYRRIRSNSLKSVISLLIASGLAIGFVFNSQNNRSVIDLLNRSFLYPRQAGESALR
                     NGLADFLPAEAQLVSLNPDNYLWEANWDRKGLYPEFYTVTAGRNFTNGSLSVTGMDHA
                     TFLQNYAGKAIPENLYVLQYAGNSERGLAKLGRVRSVDHEKIETDLVLYFISGKFPQT
                     GTVTYQRSDGNIITLNHEQAWQVRATDEGNLYQLPEQEVINFDSLDFYGF"
     gene            complement(2670..>2854)
                     /locus_tag="PK898_10270"
     CDS             complement(2670..>2854)
                     /locus_tag="PK898_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20452.1"
                     /translation="FVRKGEKEPEVITWTTWDGAGHYAKISEQWLLKETPEGRLYKLD
                     DPHAVYFDSIGLAGYQ"
BASE COUNT          862 a          723 c          570 g          699 t
ORIGIN      
        1 cggatccgtg actgtgactt gaattcggtc gcgccagact tcaacagccg atccggaaat
       61 agacgccgcc gtagtaaacg tgatcccgtc attcaccgca tctttacaac cctcgctcac
      121 cggcgtaaat tcagttgcgg cttcgaccgc gaacgtgccc gccagaacac agacaaaaag
      181 taccgctgtc accagccgga agattgattt ttttgagttc atgtaattct ccttacttta
      241 ccattctgcg gtgcacccgc ggaagtcatt gaatcatttt tatcactgta attaccgcta
      301 atcctaccat atttcccaaa ggtcttccga aaattcaaaa ttctcgaccc ttccgtttcc
      361 gttttcttca gttgcaatta tgacctttaa tcattcctcg cattaaaaat aatgacaccg
      421 gtttgtaaca aaataaacga aatccgaatc cgcttggttc caaacgcacc taaaatccat
      481 aaaaatcaag cgaatcaaag ttgatgacct cctgctcagg aagctgatat aaatttcctt
      541 catccgtcgc tctcacctgc caagcctgtt cgtggtttaa agtgataata tttccgtccg
      601 accgttgata agtaaccgtg ccggtctgcg gaaattttcc ggaaataaaa taaagaacca
      661 aatcggtttc tatcttttca tgatcgaccg agcggactct gcccaatttt gccaatcccc
      721 gttcggaatt cccggcatat tgcaatacat acaaattttc cgggatcgct tttccggcat
      781 agttttgcag aaaagttgca tgatccatac cggtcaccga cagcgaacca tttgtaaaat
      841 tgcgtcctgc ggtaacggta taaaattccg gatacaaccc ttttcgatcc cagtttgctt
      901 cccacaggta attgtcgggg ttcaatgaaa ccaattgtgc ctctgccggc aaaaaatccg
      961 ccaatccgtt acggagagca ctctcaccgg cttgacgcgg ataaagaaag gaacggttca
     1021 gaaggtcgat gaccgaccgg ttattttggg aattaaacac aaagccgatc gccaaaccgc
     1081 ttgcgatcaa caagcttatg accgatttga gagaattcga tctgatccgt cgatatcctt
     1141 cgcgaaccag gcagagcgcc aatatcccga tcccgaaata ttcgagataa acgggtaaat
     1201 aacccaagcc ggggacaatt tgcccctgat agcggtcgct caaagcgatc gtcataccgg
     1261 gcagcagcca aaaagataac ccgattcccg ccagcagacg cgtggaagta tctttttggt
     1321 tttctttacc ctttttcaga atcagtgaga aaagagaaac aattcccaac gccagcagcc
     1381 aatccgcggc ttccgtgtga cataaaatgt cccatgcccg gtcagaaaaa acctcggctg
     1441 ccggatatac attccccata atcggtatgc tgcgagtgaa aaaataaaaa ctgaaaggaa
     1501 gccccgccag aagttgcagc ccgtaagttt tcagaatttt caggacatca aaatgaaatg
     1561 cgattccgct gtaaggagtc ccttgaatca atcgattgcg gacaatcaat gccgttgtca
     1621 gtgttcccgc gaaaagaatc ccgtagggaa tgcagacttg aaccgttttc ttccgatccc
     1681 gtgtctccaa ccaagcgata acgggaatca tcaaaataaa tggaaaacaa acttcataag
     1741 tcatcaatcc gcaaaagaac ggaatcaacc cgaaccatag aaatttcttc cggttttccc
     1801 gcagatatct caacaaaaaa taggctgtca acatcagttc aagcagaatc acttgcatca
     1861 gcgaatagaa accggttacc ggatcctgat agccgcgaaa ttgaatcagg atcgggatcg
     1921 ataacatcgc agcctggccc agacgatccg aaccgccggc ataacggacc agcatgccga
     1981 aaatgatctg attaatacaa accgacagga tgatgatcag tttatagagc ttcacatttt
     2041 caatataaaa cagaaaataa tagtagcagg aaaggagatt gattctgcct aattccagat
     2101 atttccgcat aaaattgacc agattttgga tcaaaggaag ctggtcctgc ttttcaaaca
     2161 ggtaagccgt ggaatttacg gcatcatccc agtaatatcc gctatgcatc agatatcgca
     2221 aggataatgc tgtcaatata cagatcaaaa tttccacttt ccggcgttga aaaaatgcga
     2281 tcagcttgtc ttcgctaagc gaagcattgc ttcgcctcgt tatatccggc tttccgaata
     2341 aaagccacag atgcagactg acgccgaaaa agaaaaatat cagaaagaaa aaggaaaatt
     2401 tttcaagagc agccgctctt aaggaaaacc atttaacgga atcctttacc aaaaagagga
     2461 tctcccaacc tgcttccgcc catatcgcca cccaagcgat tgctcgttcg atcgattttc
     2521 cggacagata aaaatccatt ccggtaatca aaatcagcag agcaatataa taaggcaaag
     2581 cctgccggcg aatactgccg ctcatcaaac cgatcacgac ggcgatgaat atcaagagaa
     2641 agagaatccg gatggttcgt tttttcattt cattggtatc ctgctaaccc gatcgaatcg
     2701 aagtaaacag catgcggatc atctaattta tataagcgac cttccggggt ctctttcaga
     2761 agccactgtt cgctgatttt cgcataatgt cccgctccgt cccaggtagt ccaagtaatc
     2821 acttccggct ctttttctcc tttgcggaca aaga
//
LOCUS       DAOSMM010000132         2851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_35120_length_2851_cov_10.989986, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000132 DAOSMM010000000
VERSION     DAOSMM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35120_length_2851_cov_10.989986"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK898_10275"
     CDS             <1..614
                     /locus_tag="PK898_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20453.1"
                     /translation="IIAWIDTDIVNIRPHFVYGIIGPLLLDPSVKFVKGFYRRPLKIG
                     SKMQAGSGGRVTELTARPLLNLFYPELSGVIQPLSGEYAGRRDALENAVFYSGYGVEI
                     GLLIDIFEKYGLNAIAQVDLLERIHHNQELEALSKMSFAIIQTVLHKLENRYERSIMD
                     DVNKTMKLIRYNDGEYYLDIEEIAEKPRPPMISVPAYRESHHK"
     gene            611..1168
                     /locus_tag="PK898_10280"
     CDS             611..1168
                     /locus_tag="PK898_10280"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU20454.1"
                     /translation="MTRFCLVRHGQTDWNLEGRYQGQSDIPLNSTGIKQAHLIAEILR
                     DEKFDAVYSSDLSRAIRTAEIITEHRNHLKINTDPRLREVRQGEWEGLTRDIIRDRYP
                     KIWEALIENPISVCPPGGETVLEVQSRVNAALNDISALYPDGNILVVSHGLALATVIC
                     SVNGFPIGLAYQKVPENADPIWVPW"
     gene            1386..1559
                     /locus_tag="PK898_10285"
     CDS             1386..1559
                     /locus_tag="PK898_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20455.1"
                     /translation="MVRDDSPDISDESVAKGIIKIDTLKRENNSSDSCTGEVGFIFIA
                     MKFGTKKAAIPPN"
     gene            1648..1953
                     /locus_tag="PK898_10290"
     CDS             1648..1953
                     /locus_tag="PK898_10290"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOU20456.1"
                     /translation="MTWIYVSEEQELQEGNMVSVYPLGVNTVLARVNGTVYAVAGKCL
                     HMACPLFMGTLDSYILTCPCHDWRFDIRDGRFLDAPELSLKTYPTKTENGKIYIDFN"
     gene            1966..2217
                     /locus_tag="PK898_10295"
     CDS             1966..2217
                     /locus_tag="PK898_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOU20457.1"
                     /translation="MEKVTMYTLSTCPWCRKTKKFFSDHNIPFEFTDYDLADEVTQSK
                     INDEMDAAGADGFPFVMIGDKIIVGYQPDVYARLLGLDK"
     gene            2218..2559
                     /locus_tag="PK898_10300"
     CDS             2218..2559
                     /locus_tag="PK898_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOU20458.1"
                     /translation="MTIHVESRKQALRYFYTKIVEPMGYKFSPDNELVEFILEQEVNS
                     EGKYGIPFCPCQPIRHNRDHDMKIVCPCIPFHRKHFDAMRRCWCGLFVQQDVTDPDSL
                     PQLSAKEMGIE"
     gene            2552..>2851
                     /locus_tag="PK898_10305"
     CDS             2552..>2851
                     /locus_tag="PK898_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOU20459.1"
                     /translation="MSESFFQAIRTSEIAPGGIRAIEINGRQLIICNAGGKFYALSGR
                     CGHMNAPLERGTLDGTILTCPLHCAQFDIRTGEVLSGPVPMNFASEGEISSPQTRY"
BASE COUNT          816 a          607 c          657 g          771 t
ORIGIN      
        1 atatcatcgc ttggatcgat accgacattg tcaatattcg ccctcatttt gtatacggca
       61 tcattggtcc cttgctgtta gatccgtccg taaaatttgt aaaagggttt tatcgccgtc
      121 ccctaaaaat aggtagtaaa atgcaagccg gtagcggcgg tagagtgacc gaattgacgg
      181 cacgtccctt attaaacctg ttctatcccg agctctccgg cgtgattcaa ccgttgtccg
      241 gagaatatgc gggaagaagg gatgcgcttg aaaacgctgt tttttattct ggatacggtg
      301 tcgaaatagg attactcatc gacattttcg aaaaatacgg gttgaatgcg attgcccaag
      361 tcgatttatt ggaaagaatc caccataatc aggaacttga agcattaagc aaaatgtctt
      421 tcgccattat tcagactgta ttgcataagc ttgaaaaccg atatgaacgt tcgatcatgg
      481 atgacgtcaa taaaacaatg aaactgattc gttataacga cggcgaatac tatttggata
      541 ttgaagaaat cgccgaaaag ccgcgtccgc cgatgatttc ggttccggct taccgggagt
      601 cacatcataa atgactcgat tttgccttgt gcgccacgga cagacagact ggaatttgga
      661 aggtcgttat caaggtcaaa gtgatattcc tctaaattca accggcatca aacaagccca
      721 tctgattgcc gagattcttc gggatgaaaa attcgatgct gtctactcaa gtgatttgtc
      781 acgtgcgata agaacagccg aaatcatcac agaacatcgt aaccatttaa aaataaatac
      841 agatccccgc ctccgagaag tcaggcaagg cgaatgggaa ggcctcactc gggatatcat
      901 ccgagatcga tatccgaaga tctgggaagc tttaatagag aatcccattt ccgtgtgtcc
      961 tcctggcggt gaaacagtcc tggaagttca aagccgcgtt aacgccgcgc taaatgatat
     1021 ttccgctctt tatcccgatg ggaacatctt ggttgtatcc cacggtttgg cactggcgac
     1081 agtcatttgc agcgtgaacg gctttccgat cggtttggca taccaaaaag tacccgagaa
     1141 tgccgatcca atctgggtac cctggtaaat aagatattca aaatgaaaaa aaataaattt
     1201 cgatgctcaa caggtttgcg gtcagaattg atataaaact ttgaaagagc ggaattcgtc
     1261 cgctttttca tttcaataag gaatctctat taaatccgct cggtctcgac gatgggataa
     1321 ttttctatct acggattgat cggatacacg atatgtgacg atattcatcg gttaaagaag
     1381 aatagatggt ccgagatgat tctcccgata tttcggatga atctgttgca aaaggaatta
     1441 taaagatcga taccttaaaa agagaaaaca attcatccga ttcttgcacc ggtgaagtcg
     1501 gattcatttt tatcgcaatg aaattcggga ctaaaaaagc cgcgattccc ccgaattgat
     1561 tcgcttgaaa tataattgtt tttcagtgag ggctttcgat cagctctctc ttgtaaattt
     1621 ccgccgggga aagtattgag gagaaagatg acttggattt atgtttcgga agagcaagaa
     1681 ctgcaagagg gaaacatggt ttccgtctac ccgctgggtg taaatacggt cctcgcccgg
     1741 gtaaacggaa cagtatatgc ggtagccgga aaatgtctgc atatggcttg ccccttgttt
     1801 atgggcactt tggatagcta tattcttacc tgcccttgcc atgattggcg tttcgatatt
     1861 cgcgacggtc gctttcttga tgcgccggaa ctcagcctca aaacttatcc gactaaaaca
     1921 gaaaacggaa aaatatatat cgatttcaat tgaaaggaaa cttttatgga aaaagttacc
     1981 atgtatacct tatccacctg tccgtggtgt agaaaaacga agaagttttt ctcggatcac
     2041 aatatcccgt tcgaatttac ggattacgat ttagccgatg aagtcacgca gtcgaagata
     2101 aacgatgaaa tggatgccgc cggcgcggac ggattcccat tcgttatgat cggagataaa
     2161 ataatcgtcg gttatcagcc ggatgtttat gcgcgcttgt tagggttaga taaataaatg
     2221 acgatccatg ttgagagcag aaaacaggca ttgcgatatt tctacacaaa aatcgttgaa
     2281 ccgatgggat ataaattttc tccggataat gaattggttg aatttatcct ggaacaggaa
     2341 gtgaattcag aaggaaaata tggcattcct ttctgtccct gccagcctat ccgtcataat
     2401 cgtgaccatg acatgaaaat cgtctgcccc tgtattcctt tccaccgaaa gcatttcgat
     2461 gcgatgcgtc gttgttggtg cggccttttt gttcaacaag atgtcaccga tccggatagc
     2521 ctgccgcaat tatcagcaaa ggagatggga attgagtgaa agttttttcc aagcaatccg
     2581 cacttcggag atagctcccg gagggatccg agcgatagaa atcaacggtc gtcaactgat
     2641 tatttgtaat gctggcggta agttttacgc cttgagcggt cgctgcggac atatgaacgc
     2701 acctctcgaa agggggacat tggacgggac aatcctcact tgccccttac attgcgcgca
     2761 gtttgacatc agaaccggag aagttctatc cggaccggtc ccgatgaact ttgcttccga
     2821 gggagaaata tcttctccgc agacgcgcta t
//
LOCUS       DAOSMM010000133         2781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_36254_length_2781_cov_17.219736, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000133 DAOSMM010000000
VERSION     DAOSMM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36254_length_2781_cov_17.219736"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1001)
                     /locus_tag="PK898_10310"
     CDS             complement(81..1001)
                     /locus_tag="PK898_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOU20460.1"
                     /translation="MNKGLEVFSDSSERLNYNFPDFPLYVRKGTLHQFLRFAAECHWH
                     PDLEFISVLEGFMEYFIDGQIVRINSGSGIFVNSKRLHYGFSADQTDCSYIVVAVHPS
                     LLGENTYPGKAYLKEKFGPLAEDYLILDSTVSWQMEILRSMSELFDEVNQNSPNMLRL
                     LSEAVSICASVGDHIKKNSSQHPDDQSWMIFWKMVDFIHHNYDNKISLKNISEVGSVS
                     KSQCCKLFNKYVGQPPNVYLNAYRILKGCEMLQETNRSICEIALACGFQTASYFSYVF
                     RITKGENPQEYRRRVKNESKHETELSGMDR"
     gene            1119..2504
                     /locus_tag="PK898_10315"
     CDS             1119..2504
                     /locus_tag="PK898_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU20461.1"
                     /translation="MITELRSENEFDHKFNRDLFTLVLPIALQNLISAAVISADVIIL
                     GMINQSAMAAVSLAGQITFVLTLFYYGFSTGVGIQTAQYWGKKDIGTIGNILNIALIF
                     SESISILFFLLSVSIPEMLMSMFTNDSELIHYGAIFLRTASVSYLAMGLSQMYLSAIK
                     SMEKAGISAAISSFALILTIAFDALTVLVLFPHQPEKAIIGVGISTVFARFVELGLCL
                     LHSKRNNDIRIHLPRPSTIEKTLLKDFIKYTLPVQANYLVWGGALTVTTTIIGHVDSD
                     MVAANSITSAVRNLATVLCSGIAGGGAVLIGKYLGKDDLISARKAGERLNIYALFFGV
                     MAGITILISKPLILHLVNLNNTAADYLNGMLIISAYYCVGKSINSTNIGGIFPAGGDP
                     EFGFWTDTIVMWGIVLPLSYLGAFVWHIRPVLLYAIISLDEVIKLPVSIIRFRKYKWV
                     KNITRDPEPVG"
     gene            2573..>2781
                     /locus_tag="PK898_10320"
     CDS             2573..>2781
                     /locus_tag="PK898_10320"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HOU20462.1"
                     /translation="MTTMRERINSGKLFSDNCEGLPEDRENAKRKMIALNATGPNDLE
                     TRFRLIKEIFGGKADVWIEPPIYFC"
BASE COUNT          800 a          550 c          601 g          830 t
ORIGIN      
        1 gcaaatgatt ttcaatagag cttgagagtg acgtttctga aaaccaagaa taacaagaat
       61 tcgtatagaa tgatttcaac ctaccgatcc atgccggaca attctgtttc atgcttcgat
      121 tcatttttaa ctcttctgcg atattcttga gggttttctc cttttgttat tcgaaaaaca
      181 tatgaaaaat agcttgcggt ttgaaatccg caagcgagag cgatttcgca aatcgatcga
      241 tttgtctctt gtagcatttc acaacctttc aggatgcggt aagcattcag atagacgttg
      301 ggaggttgcc cgacatactt attgaacagt ttgcagcatt ggctcttgct gacagagccg
      361 acttccgaaa tatttttcag tgagattttg ttatcataat tgtgatgaat aaaatcaacc
      421 attttccaaa agatcatcca actttgatca tcgggatgtt gactcgagtt ctttttaata
      481 tgatctccga ccgatgcgca tatggaaacc gcttcggaaa gcagccgcag catattcggt
      541 gaattctggt ttacctcgtc aaataactca gacattgacc gaaggatttc catctgccaa
      601 gaaactgtgc tatctaaaat caggtaatcc tcggcaagcg gtccgaactt ttctttcaaa
      661 taggctttcc cgggataggt attttctccc aataaagaag gatgaaccgc aaccacgata
      721 taggaacaat ctgtttgatc cgctgaaaag ccataatgca gacgcttgct gttgacaaag
      781 attccgcttc ctgaatttat tcgcacaatt tgcccatcga tgaaatactc cataaatcct
      841 tcaagtaccg agataaattc aagatccgga tgccaatgac attcggcggc aaaacgcaag
      901 aattgatgta aagttccttt tcgcacgtat aaaggaaaat ccggaaaatt gtaatttaat
      961 cgttccgatg aatcagaaaa gacttccaat cctttgttca tggcttcctt tatatctgtt
     1021 tgaatacgat tttgatagaa ttctatccta ttttgggaga tattaagcag tgaatctaat
     1081 acaattttga taggttcatc attccaaaaa ggcaaacgat gattacggaa ttaagaagcg
     1141 agaacgaatt cgatcataaa ttcaacagag acctttttac tttggtcttg ccgatcgcct
     1201 tgcaaaatct aatatccgca gcggtgatct cagccgacgt aatcattttg gggatgatca
     1261 atcaatcagc gatggcagct gtttcattgg ccggacaaat aacttttgta ttaactttat
     1321 tttattatgg gttttcaacc ggagtcggta ttcaaaccgc tcagtactgg gggaaaaagg
     1381 atatcggaac cattgggaat atccttaata ttgcacttat tttttctgaa agcatttcga
     1441 ttttgttctt tcttttgtca gtatcgattc ccgaaatgct gatgagtatg tttacaaacg
     1501 acagcgaatt aattcactac ggagccatat ttttacgcac ggcttctgtt tcatatcttg
     1561 ccatgggctt gtctcaaatg tacctgagcg caatcaagag catggaaaag gcaggtatca
     1621 gcgcggcgat cagttcgttt gctctgatac ttacgattgc ctttgatgct ttaaccgttt
     1681 tggtactatt cccgcaccaa cccgaaaaag cgatcatcgg tgtcggaatc tcaacggttt
     1741 tcgcgcgatt tgtcgagctt gggctgtgct tgcttcattc aaaaagaaat aacgatatcc
     1801 ggattcactt accaagacca agtacgatcg aaaaaactct gttgaaggat tttatcaagt
     1861 acactttacc ggtccaagca aattatttgg tctggggcgg cgcattgaca gttacgacga
     1921 cgattatcgg tcatgtcgac tccgatatgg ttgcggcaaa ttcaatcaca tccgctgtca
     1981 gaaatcttgc gactgtttta tgcagcggaa tcgccggcgg tggagctgtt ctgattggaa
     2041 aatatttagg caaggatgac ctgatttcgg caagaaaagc tggggaaagg ctgaatatct
     2101 acgcactttt ctttggggtg atggccggaa taacaatcct gatatcaaaa ccgctgattt
     2161 tacatcttgt aaatctgaac aataccgccg ccgactatct caatgggatg ttgatcatca
     2221 gcgcctatta ctgtgtcgga aaatcaatca actcgacgaa catcggtggc atctttccgg
     2281 ccggcggaga tcccgaattc ggtttttgga ctgatacgat cgtgatgtgg gggatcgttt
     2341 tgccgttaag ttatttgggc gcttttgttt ggcatattcg tccggttctg ttatatgcaa
     2401 ttatcagctt agatgaggta atcaagttgc cggtatcgat cattcggttc cgaaaatata
     2461 aatgggtgaa aaatattacc cgtgatcctg aacctgtggg atagaagagt aatgacgaga
     2521 ggctgttttt tcaaaccgat tataaaaaat ttagttacaa ggagtgagaa aaatgacgac
     2581 gatgagggaa agaataaatt cgggaaagtt gttttcggat aattgtgaag gattgcccga
     2641 agatcgagaa aatgccaaaa gaaaaatgat tgcactcaat gcaaccggtc cgaatgattt
     2701 ggaaacgcga tttcgtctca tcaaagagat cttcggcggg aaagccgatg tttggatcga
     2761 accgccgatt tatttttgtt a
//
LOCUS       DAOSMM010000134         2725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_37260_length_2725_cov_10.873408, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000134 DAOSMM010000000
VERSION     DAOSMM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37260_length_2725_cov_10.873408"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1402)
                     /locus_tag="PK898_10325"
                     /pseudo
     CDS             complement(185..1402)
                     /locus_tag="PK898_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            1692..>2725
                     /locus_tag="PK898_10330"
     CDS             1692..>2725
                     /locus_tag="PK898_10330"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOU20463.1"
                     /translation="MTENQSKKNVIIIGAGPAGLTAAFELLRQEPESYNVTILEESDA
                     IGGISRTVQYNGNRMDIGGHRFFSKDQRVMDWWKERMPVQGSPSKDDILTHTVKPLDP
                     KGPDPEKEDIVMLFRNRVSRIYYLKKFFDYPISLKAQTFINMGLKNTLISGSSYLVSV
                     FHKLPETSLENFYRNRFGKKLYSMFFENYTEKVWGRHPSEISADWGAQRVKGLSVTKI
                     IIDMFSKILPAPLKKNRPVETSLIEQFWYPKFGPGQLWELTADQITQMGGIIRKNCQV
                     TGIKLVNNQVQAVTVIENGEQKEIEGDIFLSSMPLKDLVMGMGSAVPDEITRIAKGLP
                     YRDFITVGLIL"
BASE COUNT          776 a          598 c          610 g          741 t
ORIGIN      
        1 tgatcagatg acaatgaata atagaatcat tatccaataa cagcaagtcg aattgtgaac
       61 cggcacggca aaacagccgc atttgctacg aagaggaaaa acaaggagct ggcggtcgtc
      121 acttcttatc caattcaagg ccgtggaaca taaataagag gacattttcc caggaatcaa
      181 agctgacata gcgcagcaag gcttccaggt cttgaaagaa ggtttgccgt ctgcctaaag
      241 cctgacggac ggcgcggtaa tgttcattaa ccaggtctgc gacagtatgg aacaggaaag
      301 ccagtaaatt tagggaaagc agcaaggcag ccaaatattg gttcccatgt ccgaagttgt
      361 gctcaatgtg atagcctttg gtcttgagta cattgttgtg ctcattctcg accttccagc
      421 gagcccggcc atctcgcaca atggcctcta ccgtcatcgc agtgatctca aaatccgtga
      481 taaaggcgtt tttgtaaatg agttcgccgg tatcttcccg aacaatgctc aattcgcacc
      541 agtttactgc taatgcgttt cgctctcctc ctaagggaag ttggttggca tagcgatagg
      601 tatagatttc tacataacgt ccattccatt ttcgcacctc tttgcgtcct aaaacatgct
      661 gaacgcttag aaaatccacg gtttcgtaga agtggagatg cgaatccggc ttgcagacga
      721 gaataaagtg gcaatgtttg tcctttagtg cctgacaaaa aggttggcgg ctgtacagat
      781 cgtcacccag gatagtgata tgctgttggg cgtagaaagc agcatgcttt tcaagccaac
      841 gcttggcagc ccgaatttcg caatcttgct tttcagcccc atcctgtggg gtaataaact
      901 ccggctccag gggaagcacg cgctcatttc ccgcctgcac aatcaccggc gtcagcacac
      961 tgtggtaata aagggtttcg ccattctgta aggtgcgatg actgcattgc tcacaatgga
     1021 ttttcttcga tgagaagtat tccgtcccat cgatcgctac taatagggta ttcccaaacg
     1081 agtgataccc ttccagtatt ccggcttgct ccaggtcttg aaatagctgc tggtagcatc
     1141 tatctaggtg ttctggactg atcggatcca gcaggttgcg aatttgattg tcactgggta
     1201 tctcgcggag gtcaaacaaa ttctccgcat tgctgcggcc cttacgcaac ttcatgtctc
     1261 gctgatgtgc taaaaacgat ggactttggg taaaaaatac cgaaaatgcc cccattccag
     1321 catcagctaa ctcataatgg ttgttctttc cctgacgatg ttctggaaga ccttcaaaaa
     1381 tcgcttccaa actttggagc atgtggtcga gttttaattt ctggttggtc atgccctaaa
     1441 aggcgcaatt tccgtgccat tttgattgca ttatcctaat ttgtaatttt tcaaattgag
     1501 aattgctgtt gtttttcaac accctcataa caatagcctt gacactggat tgaatatgtg
     1561 atccaataaa aataaatcga ctctctcaag agatccaatt cattttttta tcaattcgga
     1621 tcagttataa tcgcattgtc gaatggatga ccgaatcatg acaagttcaa aataatgtat
     1681 caggatgcaa tatgacagaa aatcaatcaa agaaaaatgt aattattatc ggcgcaggcc
     1741 cggcaggatt gacagccgct tttgaacttc tcagacagga accggaatct tataacgtta
     1801 ccattctgga agaatccgat gccatcggcg gtatttccag aaccgttcaa tataacggca
     1861 atcgtatgga tatcggggga catcgttttt tctcgaaaga tcaacgggtg atggattggt
     1921 ggaaagaacg aatgccggta cagggaagcc cttccaagga tgatattctg actcacaccg
     1981 taaagccgtt ggatcccaaa ggaccggatc cggaaaaaga agatatcgtc atgctttttc
     2041 gcaatcgcgt ttccagaatt tattatctga agaaattttt tgactatccg atttcattaa
     2101 aagcccaaac ttttatcaat atggggttaa aaaatacgct gatatccggt tccagttatt
     2161 tggtttccgt tttccataaa ttaccggaaa cttcactcga aaacttttac cgtaaccggt
     2221 tcggaaaaaa attgtattcg atgttctttg aaaattacac ggaaaaagtc tggggcaggc
     2281 atccttcgga aatctccgcc gattgggggg ctcaacgggt taaaggattg tccgtcacaa
     2341 aaattatcat tgatatgttt tcaaaaattt tgccggctcc cttgaaaaag aaccggccgg
     2401 ttgagacttc actgatcgaa caattctggt atccgaaatt tggacccggc caactttggg
     2461 aacttacagc ggatcagatc actcaaatgg gaggtatcat ccgaaaaaat tgccaagtaa
     2521 ccggtatcaa actcgtaaac aatcaagtac aagcggtgac ggttattgaa aacggagaac
     2581 aaaaggaaat cgaaggcgat atttttctct ccagtatgcc gctaaaagat ttggtcatgg
     2641 gaatgggatc ggcagttccc gatgaaataa cgagaatcgc aaagggattg ccttaccggg
     2701 actttattac cgtcggtttg attct
//
LOCUS       DAOSMM010000135         2715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_37448_length_2715_cov_9.670301, whole genome shotgun sequence.
ACCESSION   DAOSMM010000135 DAOSMM010000000
VERSION     DAOSMM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37448_length_2715_cov_9.670301"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PK898_10335"
     CDS             complement(<1..716)
                     /locus_tag="PK898_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU20464.1"
                     /translation="MSFVAGIGRANIDLLYSGLSHIPREGEEVYSKDFDLQLGGGPVA
                     TMINLQRLGVPVKVLTFLGKDMFSAFAEQRLHEYGIEAINLYGGGKIPVNISTAMITG
                     RDRTFVSYNQLQQTTADQLKIAESEIEGAKIVEMQPGFLELYRKLKQKGTLLVLDMGW
                     EDDLSFEKYRDYFELADYYTPNLKEALYLTGTDNPLDALLILSQWFTKPIVKIGDQGC
                     LIKDEEGIWQIPHIDEFEST"
     gene            complement(733..2085)
                     /locus_tag="PK898_10340"
     CDS             complement(733..2085)
                     /locus_tag="PK898_10340"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20465.1"
                     /translation="MKLTVIGGGGVRSLFLAKSIALEARELNIDELVLMDTNDQKLEI
                     FGSLAMEAAIRLAPHLKVSLTTDAREAVRDADYVITTIRVGEDEARVADEKIALDLHL
                     LPQETTGACGFSFAMRSIPVLADYCGLIRDIAKPTVQVFNFTNPAGLVSQALRDAGFD
                     FTYGICDAPAGLLSQIADLENVSTDHLDMDCYGLNHLSWFPAVRLDGKDITQELIRDD
                     RLYHETDMKFFEPALAQSYQALFNEYLYYYYYREQAVENIRNAKQTRGQLIEQVNRQM
                     ILDFSEHPHLSFEERLKIFEHYYGIREDAYMASETGKKREKTFRFDLTAPDTGGYAGV
                     ALNFIRIQQQGIPGRMILSVPNRGSLPFLEQNDVAELSCIVDSDGVHPDPPPNFHPVA
                     CELIRRMKLYERWAAEAIRCRNFDLSVRALQIHPLIESYSLAKSLATSFFKLNEPWIK
                     "
     gene            complement(2095..>2715)
                     /locus_tag="PK898_10345"
     CDS             complement(2095..>2715)
                     /locus_tag="PK898_10345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU20466.1"
                     /translation="LMPEWAKAMGDRVFYGNGEYPSKDTIEAEAIHVVEKHPRLRIIF
                     AHFFFIADDLEKAAGYFERFPNLMFDITPGWEMFESFANRYEESRDFFRKYADRIIFG
                     TDTISDHWRTTVGNLRRVLETDDRFVSFEENCHGLNLDEKTLRQIYIENYHKFLPAKP
                     AEMKIDEIIAYADRLSVRIAAEAPAETPAILAEIKNCRQELDRLRD"
BASE COUNT          742 a          694 c          547 g          732 t
ORIGIN      
        1 tccgtcgatt cgaattcgtc gatatgcggg atctgccaga ttccttcctc gtccttaatc
       61 agacatccct gatctccgat tttgacaatt ggtttggtaa accattgaga aagaattaat
      121 aatgcatcca acggattatc cgtgccggtt aaatagagcg cttctttgag attgggcgtg
      181 taataatcag ccagttcaaa ataatcacgg tatttttcaa aactgagatc atcttcccaa
      241 cccatatcca aaaccaacag cgtccctttt tgtttgagct ttcgatacaa ttcgagaaat
      301 ccgggttgca tctcaacaat tttggctcct tctatctccg attcggcaat tttcagttgg
      361 tcagcggtag tttgctgaag ctgattgtaa gaaacaaaag ttcgatctcg gccggtaatc
      421 atcgcggtgg aaatattcac cggaatcttc ccgccgccgt acagattgat cgcttcgatg
      481 ccatattcat gcagccgttg ttcggcgaat gccgaaaaca tatcttttcc caagaaggtt
      541 aaaactttta ccggcacgcc cagtcgctgt aaattgatca tcgtcgcaac cggtcctccg
      601 cccagttgaa gatcaaaatc ttttgaatag acttcttccc cttcacgcgg aatatgtgat
      661 aatcccgaat aaagaaggtc aatatttgct ctgccgattc cggcgacaaa actcattctt
      721 tcttcccccg atttatttta tccatggttc gttcaattta aaaaatgacg ttgccaatga
      781 ttttgcaagc gaataagatt cgattaatgg atggatttgc aacgcccgaa cagacaggtc
      841 aaaatttcgg cagcgaatcg cttccgccgc ccaacgttca taaagcttca tccgccgaat
      901 caattcacaa gctaccggat gaaaattcgg cggcggatcc ggatgaacgc cgtccgaatc
      961 gacaatacag ctcaattccg ccacgtcgtt ttgctctaaa aacggtaatg atccgcgatt
     1021 cgggacactt aatatcattc gtcccggaat accctgctgc tgaatgcgaa taaaattgag
     1081 agctaccccc gcataaccgc cggtatccgg cgctgtcaaa tcaaagcgaa atgtcttttc
     1141 tcgctttttc cccgtctcgg aagccatata ggcatcttcg cggattccgt aatagtgttc
     1201 aaagattttc aaacgttctt caaatgagag atgaggatgc tcggaaaaat ccagaatcat
     1261 ctgacgattg acctgttcga tcaactgtcc gcgggtctgt ttcgcatttc gaatattctc
     1321 aaccgcttgt tcccgataat agtaataata cagatactcg ttgaataatg cctgatagga
     1381 ttgtgccaat gccggttcaa agaatttcat atcagtttca tgatataacc gatcgtcccg
     1441 aatcaattcc tgcgtaatat cttttccatc cagccgaacg gcgggaaacc atgagagatg
     1501 attcaacccg tagcaatcca tatccagatg gtccgtcgaa acgttctcca aatccgctat
     1561 ctgagaaagc aacccggccg gtgcatcaca tatgccatag gtgaaatcaa agccggcatc
     1621 cctcaatgcc tgagaaacta agccggcagg attggtaaaa ttgaaaacct gcacggtggg
     1681 ttttgcgata tcccgaatca agccgcaata atccgccaac accggaatgg agcgcatcgc
     1741 aaacgaaaag ccgcaggcgc ctgtcgtttc ttgtggaagc aaatgaaggt cgagggcaat
     1801 tttttcgtcc gccacacggg cttcatcttc gcctacacga atcgtagtaa tcacataatc
     1861 cgcatctcgg accgcttctc gagcatctgt agttaacgaa actttcaaat gaggggctaa
     1921 ccgaatcgcc gcttccattg ccagcgaacc gaaaatctcc aatttttgat cattggtgtc
     1981 cattaaaacc aattcatcaa tattcagctc ccgggcttcc aacgcgatac tcttcgctaa
     2041 aaatagcgat cggacgccgc caccgccgat gacagttaac ttcatattcc cctcttaatc
     2101 tctcaaccga tcgagttcct gcctgcaatt ttttatttcc gctaaaattg ccggcgtttc
     2161 cgccggtgct tcggctgcga tgcgaacgga aaggcgatct gcataagcta tgatttcatc
     2221 aatcttcatt tcggcaggtt ttgcgggtaa gaatttatga taattttcga tataaatctg
     2281 ccgtaatgtt ttttcatcca agttcaaccc atgacagttc tcttcaaagg aaacaaaacg
     2341 atcatccgtc tccagcactc gccgcagatt gccgactgtt gttctccaat gatcggaaat
     2401 cgtatccgtt ccgaaaataa ttcgatcggc atacttcctg aaaaaatccc gcgactcttc
     2461 ataacggttg gcaaaacttt caaacatctc ccacccgggt gtgatatcaa acatcagatt
     2521 cggaaatcgt tcgaaatatc cggcagcctt ttcaagatcg tctgcgataa agaaaaaatg
     2581 agcaaaaata attcgcaacc ggggatgctt ttcgaccaca tgaatagcct cggcttcgat
     2641 cgtatctttg gaaggatatt cgccgttgcc gtaaaaaaca cggtcgccca ttgcttttgc
     2701 ccattcgggc atcag
//
LOCUS       DAOSMM010000136         2695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_37835_length_2695_cov_13.520076, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000136 DAOSMM010000000
VERSION     DAOSMM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37835_length_2695_cov_13.520076"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PK898_10350"
     CDS             <1..734
                     /locus_tag="PK898_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU20467.1"
                     /translation="TALLLFFTYKSKSPVAATFNTLFDFDRNLIIKTARNVLPVLINE
                     TLWGLGTNIYSAIYAKISTTAIAAVSAVSPVDTLMFTLIYSFGDACGILVGNLMGRNE
                     QEKAYEYGIFTAFFMMIIGFLTGVVVFFLRDPILNLYNLSAESYASARIILTILTASL
                     GFRAITYTIFIGVLRAGGDVRFCLAVDAGSIWLIGIPVSVVLAFGFHLPIETVYLGFM
                     CDELFKVIVVTHRLRTRKWMNVLTA"
     gene            complement(723..1325)
                     /gene="cyaB"
                     /locus_tag="PK898_10355"
     CDS             complement(723..1325)
                     /gene="cyaB"
                     /locus_tag="PK898_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOU20468.1"
                     /translation="MEEELTKNSGKNQEIEAKFPVKDLRKIRELLELRQAELLMDKSY
                     EHNILLDTPNNELLAENARLRLRKEGSESILTYKRTRKNENNIAYREEIETRVDHFEN
                     TRLILNRLGFLTFFEYEKYRSTYRLGATTIMLDETPIGFYLEIEGPDEETIHRTASLL
                     EIDWNQRTDKSYLQVFQEWAAENGYTGRDMLFSSAPFLRG"
     gene            1983..2192
                     /locus_tag="PK898_10360"
     CDS             1983..2192
                     /locus_tag="PK898_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOU20469.1"
                     /translation="MSDKVKGTVKWFNESKGYGFLSQPEGEDIFVHYTAIQGNGFRTL
                     KEGAEVEFNIEHGPKGLQASNVIVL"
     gene            complement(2277..>2695)
                     /locus_tag="PK898_10365"
     CDS             complement(2277..>2695)
                     /locus_tag="PK898_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOU20470.1"
                     /translation="LTVQKEVAQRICAQPGDLSLLALSVQIFGSVSIPLAIPAGAFFP
                     QPKVDSAVVKIDVYDEPLVSAAESDDFFKLAKAGFGQKRKTLRNSLSSDLRLSTFEVE
                     AALIEAGINPMRRAETLSIEEWKRLVDSVRENKNSR"
BASE COUNT          734 a          603 c          571 g          787 t
ORIGIN      
        1 aaaccgcatt attgcttttc ttcacttaca aatcaaagtc cccggtcgct gcaacattca
       61 acaccttatt tgattttgac aggaacctga ttattaaaac agccaggaat gtacttcccg
      121 tattgattaa cgaaactctt tgggggctcg ggacgaacat ctattcggca atttatgcca
      181 aaatttcgac gaccgccatt gccgctgtct ctgcagtcag tcccgtggac actttgatgt
      241 ttacgctgat ttattctttc ggcgacgcct gcggtatttt agtcggaaat ctcatgggga
      301 gaaacgaaca ggaaaaagcc tacgagtacg gaatctttac cgcatttttc atgatgatca
      361 tcgggttcct gaccggagtc gtcgtatttt tcctgagaga tccaattctg aacctctaca
      421 acctctccgc ggaatcttat gcaagcgcaa gaataattct gacgatacta accgcatcgc
      481 tgggttttcg ggcgattact tatacgattt ttatcggtgt tttgcgggcg gggggagatg
      541 tcagattttg cttggcggtg gacgccggct ctatttggct catcggaata ccggtcagcg
      601 ttgttttagc cttcggtttt cacctgccga ttgagacggt ctatttgggt tttatgtgcg
      661 atgaactgtt caaagtgatc gtcgtcactc atcgtttgcg aacccgaaaa tggatgaatg
      721 tcttaaccgc gtaaaaaggg agccgagctg aaaagcatat cgcgaccggt atagccgttt
      781 tcggcagccc attcctgaaa tacctgcagg taacttttat ccgtgcgctg attccaatcg
      841 atctctaaca gagaagccgt acgatgaatg gtctcttcat cgggtccttc tatttccaga
      901 taaaaaccga tcggcgtttc atccagcatg atcgtagttg ctcccaacct gtaagtactg
      961 cgatattttt catattcaaa aaaggtcaga aaacccaaac ggtttaatat caggcgggtg
     1021 ttttcaaaat gatcgacccg ggtttcgatt tcttctcgat aagcgatatt attttcattt
     1081 tttcgggtac gtttatatgt caatatagat tccgaaccct cttttcgcaa gcgtaatcgg
     1141 gcgttttccg ccagcaattc attgttcgga gtatccaata aaatgttatg ttcataggat
     1201 ttgtccatca gcaattcggc ttggcggagc tccaacagtt cccgaatttt ccgcaaatcc
     1261 ttcaccggga actttgcttc aatttcctga ttttttccgg aatttttcgt aagctcttct
     1321 tccataacta tcgattttac ctctttttca ggttaaataa cttcacggaa aaagaccgga
     1381 aatggttttt ctgtcatttt ccccttcgat ttttatctca tttctttccc cacgaacgac
     1441 tttattattg taattataat tttatcgaag gatcctatat agtaaaatca tggagcgagg
     1501 cagggaaaac agaaaatgtt tatccgaacc ggttcgagta catttttcga cgtagaaatc
     1561 tttctatctc aattcagtcc ggtgaagata atttgaaata tatcagtccg catcatcggt
     1621 cccgctgttt tgggcgacct tgtcagcttt atcggctcat ggtcccctta aaaaatttca
     1681 tctgaaagaa ataatcatgc tctgtaaaaa catcaagggg aacatgcatg cgcagaaccg
     1741 ttacattgat aatgaatctt gacgatgttt gtaatatgcc cttcgacaaa agaaaaaata
     1801 atggtataaa tagagcagtg attaaggaga ttgtgtaatg aagtgccgct ttcctcaatt
     1861 tacatgggaa agcggttttc tgtcaacaac ttcttaacaa aatttagtca atggaatggc
     1921 caaatgcctt ccaaattttc aaacctcggg gagccgaggg aaattttcag gagattttca
     1981 gaatgagtga caaggtcaaa ggtacagtca agtggttcaa tgaatcaaag ggttatggtt
     2041 ttctttcaca gccggaaggt gaagatattt tcgttcacta caccgcaatt cagggcaacg
     2101 gattccgcac tctgaaagaa ggcgcagaag ttgaattcaa tattgaacac ggtccgaaag
     2161 gtctgcaggc cagcaatgta attgttctct gatcgaacag agaattaaac cttcgggaaa
     2221 tgaagttttt tgtaatttca aatctgtacc aaggtattag aaataaacag ccgattttat
     2281 cggctgtttt tattttcccg gaccgaatcg accagacgtt tccattcctc aatcgacagc
     2341 gtttcggcgc gacgcatcgg attgatgccg gcttcgatta aagcagcttc gacctcaaaa
     2401 gtcgaaagcc ggagatcgga agacaacgaa tttcgcaatg ttttccgctt ctgaccgaac
     2461 cccgcttttg ccaacttaaa aaaatcatcc gattcggcag ccgaaaccag cggttcgtca
     2521 tatacatcaa tctttaccac agccgaatcc actttgggct gcgggaaaaa agcaccggcc
     2581 ggaatcgcca acggaataga tacggaaccg aaaatctgca cgctcaatgc caacagactg
     2641 agatccccgg gttgcgcgca aatacgctgc gcaacctctt tctgaaccgt cagga
//
LOCUS       DAOSMM010000137         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_39434_length_2607_cov_7.025470, whole genome shotgun sequence.
ACCESSION   DAOSMM010000137 DAOSMM010000000
VERSION     DAOSMM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39434_length_2607_cov_7.025470"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK898_10370"
     CDS             <1..514
                     /locus_tag="PK898_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU20471.1"
                     /translation="HPRLRIIFAHFFFIADDLEKAAGYFERFPNLMFDITPGWEMFES
                     FANRYDESREFFRKYADRIIFGTDTISDHWRTTVGNLRRVMETDEYFVSFEENCHGLN
                     LDEKTLRQIYIENYHKFLPAKPATMKVDEIIAYADRLSDRIAAEAPAETPTILAEIKN
                     CRQELERLKD"
     gene            523..1875
                     /locus_tag="PK898_10375"
     CDS             523..1875
                     /locus_tag="PK898_10375"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20472.1"
                     /translation="MKLTVIGGGGVRSLFLAKSIALKARELNIDELVLMDTNDQKLEI
                     FGSLAMEAAIRLAPHLKVSLTANAREAVRDADYVITTIRVGQDEARVADEKIAMDLHL
                     LPQETTGACGFSFAMRSIPVLLDYCSLIRDFAKPSVQVFNFTNPAGLVSQALRDAGFD
                     FTYGICDAPSGLLSQIADLKNVSVERLDMDCYGLNHLSWFPAVRLDGEDITRELIRDD
                     RLYHETDMKFFEPELVQSYQALFNEYLYYYYYREQAVENILNAKLTRGQLIEQVNHQM
                     IRDFSEHPERSFEERLKVFEHYYGIREDAYMASETGKKREKAFHFDLSAPDAGGYAGV
                     ALNFIRMRQQGYMGRMILSVPNQGSLPFLEQNDVAELSCIVDSNGVHPDPPTNFHPVA
                     CELIRRMKLYERWAAEAICSRNFDLSVRALQIHPLIESYSLAKSLAKSFFKLNEPWIK
                     "
     gene            1892..>2607
                     /locus_tag="PK898_10380"
     CDS             1892..>2607
                     /locus_tag="PK898_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU20473.1"
                     /translation="MSFVAGIGRANIDLLYSGLSHIPREGEEVYSKDFDLQLGGGPVA
                     TMINLQRLGVPIKVLTFLGKDMFSAFAEQQLQEYGVEAINLYGGAKIPVNISTAMITN
                     RDRTFVSYSQLQQATADQLEIAESAMDGAKIVEMQPGFLELYRNLKQKGTLLVLDMGW
                     EDDLSFEKYRDYFDLADYYTPNLKEALYLTGTDNPLDALKILSQWFTKPIVKIGDQGC
                     LIQDEEGIWQIPHIDEFEST"
BASE COUNT          715 a          484 c          655 g          753 t
ORIGIN      
        1 tcatccccgg ctgcgaatta tttttgctca ttttttcttt atcgcagacg accttgaaaa
       61 ggctgccgga tatttcgaac gatttccgaa tctgatgttt gatattacgc ccgggtggga
      121 gatgtttgag agttttgcca atcgatatga tgaatcacga gaatttttca ggaaatatgc
      181 tgatcgaatt attttcggaa cggatacgat ttccgatcat tggaggacca cggtcggcaa
      241 tttacggcga gtgatggaaa cggatgaata ttttgtttct tttgaagaga attgtcatgg
      301 gttgaatttg gacgaaaaga cgttgcgaca gatttatatt gaaaattatc ataaattttt
      361 accagcgaag ccggccacga tgaaggttga tgaaatcata gcttatgcgg atcgtctttc
      421 cgatcgcatc gcagccgaag caccggcaga aacgccgaca attttagcgg aaataaaaaa
      481 ttgcaggcag gaactagagc gattgaaaga ttaagaggaa atatgaagct aactgtcatc
      541 ggcgggggcg gtgtccgatc cttattttta gcgaaaagta tcgctttgaa agcccgggag
      601 ctgaatattg atgaattggt tttaatggac accaatgatc aaaaattgga gattttcggt
      661 tcgctggcaa tggaagcggc gattcggtta gcccctcatt tgaaagtttc tttgaccgcc
      721 aatgctcggg aagcggtccg ggatgcggat tatgtgatta ctacgattcg tgtaggccaa
      781 gatgaagccc gtgtggcgga tgaaaaaatt gctatggatc ttcatttact cccgcaggaa
      841 acgaccggtg cttgcggttt ttcgtttgca atgcgctcca ttccggtctt attagattat
      901 tgcagcttga tacgggattt tgcaaagcct tctgttcagg ttttcaattt tactaatccc
      961 gccggcttag tttctcaagc attgagggat gccggctttg atttcactta tggaatttgt
     1021 gatgcgccaa gcgggctgct ttctcagata gcggatttga aaaatgtctc ggtggaacga
     1081 ttggatatgg attgctacgg tttaaatcac ctttcatggt ttccagctgt tcggctggat
     1141 ggggaagata tcacccggga actgatccga gatgatcggt tgtatcatga aacggatatg
     1201 aaattcttcg aaccggaatt ggtgcaatcc tatcaggcat tatttaatga atatctgtat
     1261 tattactatt atcgggaaca agcggttgaa aatattctga atgcgaaact tacccgcgga
     1321 caattgatcg aacaggtaaa tcatcagatg attcgggatt tttcggagca tcctgagcga
     1381 tcatttgaag agcgcttaaa agtttttgaa cattattatg gcatccgtga ggatgcttat
     1441 atggcttccg agacagggaa aaaacgagaa aaggcatttc actttgattt atctgcgccg
     1501 gatgccggcg gttatgcggg tgtagccctt aattttatcc gcatgcggca gcagggctat
     1561 atggggcgaa tgatattaag tgtcccgaat caaggatcat taccgttttt agagcaaaat
     1621 gatgtagcgg aattgagttg cattgtcgat tcgaacggtg tgcatccgga tccgccgaca
     1681 aattttcatc cggtggcttg tgaattgatt cggcggatga agctttatga acggtgggct
     1741 gccgaagcga tttgcagccg aaattttgac ctgtctgttc gggcgctgca aatccatcca
     1801 ttaatcgaat cttactcact tgcgaaatca ttggcaaagt cattttttaa actgaacgaa
     1861 ccatggataa aataaatcgg gggaagaaag aatgagtttt gtcgccggaa ttggcagagc
     1921 aaatattgat cttctttatt cgggattatc acatattcca cgtgaagggg aagaggtcta
     1981 ttcaaaagat tttgatcttc aactgggcgg ggggccagtt gcgacaatga tcaatttgca
     2041 gcgtctggga gtgccgataa aagttttaac ttttttggga aaagatatgt tttcggcatt
     2101 tgccgaacaa caattgcaag aatatggtgt tgaagcgatc aatctatacg gtggcgcgaa
     2161 aattccggtg aatatttcta ccgcgatgat tacgaaccgg gatcggactt ttgtctctta
     2221 tagtcagctt caacaagcca ccgccgatca attggaaatt gccgaatcag cgatggatgg
     2281 tgcaaaaatt gttgaaatgc aacccggatt tctcgaattg tatcgaaatc tcaaacagaa
     2341 ggggacgctg ctggtcttgg atatgggttg ggaagatgat ctcagttttg aaaaataccg
     2401 ggattatttt gatctggctg attattacac gcccaatctc aaagaagcgc tctatttaac
     2461 cggcactgat aatccgttag atgcattaaa aattctttct caatggttta ccaaaccgat
     2521 tgtcaaaatc ggcgatcaag gatgtctgat tcaggacgag gaaggaatct ggcagatccc
     2581 gcatatcgac gaattcgaat cgacgga
//
LOCUS       DAOSMM010000138         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_42211_length_2478_cov_18.573669, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000138 DAOSMM010000000
VERSION     DAOSMM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42211_length_2478_cov_18.573669"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PK898_10385"
     CDS             complement(<1..347)
                     /locus_tag="PK898_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20474.1"
                     /translation="MKNRNGIEVGFNSAGASIAYVLTPDRYGNFKNIALTLPEPKDYL
                     NNEIYAGATVGPVAGRIKDGILPVLDQTYHLCRNEGNNTLHGGEKNFAHVIWNMTDFR
                     ENEDEASLRFSYTA"
     gene            684..2192
                     /locus_tag="PK898_10390"
     CDS             684..2192
                     /locus_tag="PK898_10390"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU20475.1"
                     /translation="MDNLQEENILEFSEISKYFPGVKALDNVSFTARSGEVLAFLGEN
                     GAGKSTLLKILNGDYQPDKGKYLINGVEKKFNSPQEAIEEGISVIYQERQILMELTVA
                     ENIFLGRMPVNNFGIINVKKANKMAAAIIKDFGLPINPTTKVKDLSIAFQQMVEIMKA
                     YSRENLKVICFDEPTASLSDSEIDILFGIIRKLKKENKIIIYVSHRLKEIQQITDRVA
                     IFKDGKYITTIKTGEVSEKEMIKMMVGRDLGDIFLNLDRNKEIGDVLLDVKDVSSEFV
                     KECSFQLRKGEVLGFSGLVGAGRTELMRAVIGADRRKGGEISLEGKKIHNSSPKEAMK
                     NGIVLVPEDRKMQGILSNLNVSGNINISLLDKNANKFGIIDSKKELQIAEKCIDEFHI
                     KTPSPLKRIVELSGGNQQKCIVARGISTMPKVLILDEPTKGIDVGTKSEFYQMICAFA
                     KQGLGIILISSELPEVLGLSDRIIVMRSRKIVGEINRETATEALVLSMSMID"
     gene            2228..>2478
                     /locus_tag="PK898_10395"
     CDS             2228..>2478
                     /locus_tag="PK898_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20476.1"
                     /translation="MEEEKGIKKTITRITNFIGNDKLVLLIAIIVVFAMFTFLNPNFM
                     SKQNIINLLVAGSLIGLVAIGHTYLIIAGQNDLSPGSLAA"
BASE COUNT          796 a          463 c          525 g          694 t
ORIGIN      
        1 gccgtatagg aaaaccgaag cgaagcctca tcttcatttt ccctaaaatc ggtcatattc
       61 caaataacat gggcgaagtt cttttctccg ccgtgcagag tattgtttcc ctcgtttcgg
      121 cagagatgat acgtctggtc caaaaccgga aggatgccgt ctttgatacg acccgctacc
      181 gggccgactg ttgccccggc atatatttca ttattcaaat aatcttttgg ttccggtaaa
      241 gtaagcgcaa tatttttaaa attaccgtaa cgatccggtg tcaaaacata ggcaatcgag
      301 gcacctgccg aattgaatcc cacttcaatt ccgtttctgt ttttcattcg aaaagtccgc
      361 ataaagttat tccttccgtc aggattattc cttttatgat cctttttagg gagcacaaat
      421 gttagtatag accgaaatta tcaaccggat atcccgatgg taaaaatagc aatttattta
      481 aatagcatcg caacattcta taacaaacag ttattattct gaagtatgat gaaatcagat
      541 atttttatta ttatgatatt ttcaaagaat atcataataa aatttttaat agtctaatat
      601 ttacaagatc ttctttcgga agcataaata ttttttacgg tttgggcata tccaaataaa
      661 aggtttatat aggattcaca ccgatggata accttcagga agagaacatt ttagagtttt
      721 cagaaatttc aaaatatttt ccgggcgtca aagctctgga caatgtttct tttaccgccc
      781 gcagcggtga agttttagca tttctgggag aaaacggagc cggaaaatcc actcttctta
      841 aaattctgaa cggcgattac caaccggata aagggaaata tttgattaat ggcgttgaaa
      901 agaaattcaa ctctccccag gaagcaatcg aagagggaat cagcgtcatt tatcaggaac
      961 gtcagattct gatggaactg accgttgccg aaaatatttt tctgggaaga atgccggtaa
     1021 acaatttcgg aattattaat gtaaaaaaag cgaacaagat ggctgccgcc attattaagg
     1081 atttcgggct gccaatcaat ccgaccacaa aagttaagga tttgagcatc gcctttcaac
     1141 aaatggtcga aattatgaaa gcatacagcc gggaaaatct caaagtgatc tgctttgatg
     1201 agccgacggc aagtctgagt gactcggaaa ttgatatcct cttcggaatc atccgaaaac
     1261 tgaagaaaga aaataaaatc attatttatg tttcccatcg tctaaaagaa attcaacaga
     1321 ttactgatcg ggttgccatt tttaaagacg ggaaatacat cacgaccatc aagaccggtg
     1381 aagtgtcaga aaaagaaatg atcaaaatga tggtcggtag agatctgggt gatatcttct
     1441 taaatcttga tcgaaataaa gagatcggag acgtattact cgacgttaag gacgtctctt
     1501 cggaatttgt caaagagtgc tcttttcaac tgcggaaagg agaggtcctc ggcttcagtg
     1561 gtttggtagg tgccggcagg accgaactga tgcgtgccgt gatcggcgcg gacagacgaa
     1621 aaggcggaga aatatctttg gaaggcaaaa agattcataa ctcttcgccg aaggaagcga
     1681 tgaaaaacgg tatcgtactc gttccggaag accgaaaaat gcaggggatt ctgtcaaatt
     1741 taaatgtttc cggaaatatt aatatttctc tgctcgataa gaatgcaaat aaattcggaa
     1801 ttattgacag caaaaaggaa ctgcaaattg cggaaaaatg tatcgatgaa tttcacatca
     1861 agacgccatc cccgctaaaa agaatagtcg aattgtccgg cgggaatcaa cagaaatgca
     1921 tcgtggcgcg cggaatttcc acgatgccca aagtgttgat tttggatgag ccgaccaaag
     1981 ggattgatgt cggaacaaaa tccgagtttt atcaaatgat ttgtgcattt gcaaaacaag
     2041 ggctcggaat cattctcatt tcttccgaat tgcctgaagt tttaggtctt tccgatcgaa
     2101 ttattgtcat gagatccaga aaaattgtgg gtgaaataaa tcgggaaaca gcaaccgaag
     2161 cattggtttt aagtatgagc atgattgact aaatcgctga aaaggattga aaataccgga
     2221 aatgactatg gaagaagaaa aaggaatcaa gaaaacaatc actcgtataa caaactttat
     2281 cggcaacgat aaattggtat tgctgatcgc gattattgtc gttttcgcaa tgtttacttt
     2341 tctgaatccc aattttatga gcaagcagaa catcatcaat ctgttggttg ccggttcgct
     2401 catcggattg gtagcaatcg gtcatactta tttaattatt gccggacaaa atgatttgtc
     2461 gcccggctct ttggctgc
//
LOCUS       DAOSMM010000139         2445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_42995_length_2445_cov_16.821757, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000139 DAOSMM010000000
VERSION     DAOSMM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42995_length_2445_cov_16.821757"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1226
                     /gene="recF"
                     /locus_tag="PK898_10400"
     CDS             33..1226
                     /gene="recF"
                     /locus_tag="PK898_10400"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOU20477.1"
                     /translation="MILQSLSLTNFRSYVRLDVEFPQGITILSGGNAQGKTSLLESLF
                     YCATFATILAGNDRQLINFEAQKQDLAVARIVVRYSRDQQNHQLEVRIIQESQNRITR
                     TRKEILWNGQKLRAQNAIGKFPAVLFIPQMTAILEGPPQERRRYLNILLSQSEPGYAQ
                     TLTEYKQALVQRNALLRQIADKKSDPAQLEYWDELLSSRGAVLIQKRIKALETLNEYA
                     EEIHLQLTDRQEKLALIYNPSFDPLNPDRSNPVEENSGRRDGFSADEIASAMKAHLKT
                     IRRREIERGTSVIGPHRDDFQVCANDIDLSLYGSRGQIRTALLSLKMAEIRWIQDKTG
                     TEPLLLLDETLAELDEKRRRDLLERLDQYNQGILTTTDLTHFSDDFLKKHTVWQIANG
                     VVQKD"
     gene            complement(1223..1945)
                     /gene="rsmG"
                     /locus_tag="PK898_10405"
     CDS             complement(1223..1945)
                     /gene="rsmG"
                     /locus_tag="PK898_10405"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOU20478.1"
                     /translation="MQHMSEWIDIAAQFIGFPLSEDRLRLFEIYESELIEWNTRFNLT
                     AIKNPDEIRTKHFLDSLSCVLAINDFNGRSLVDVGTGAGFPGIPLKIVFPDLKLTLVD
                     SVGKKLVFCQHIIDRLSLTDCRIIHARAEDLGKDPLHRERYDIAVARAVTGLSALSEL
                     LLPLVKRKGIMIAQKGETAAEELKAAEKAIRILGGGETQLIPVTIPNAAEPRYLIRIE
                     KQRNTPSGYPRRPDQIRKKPID"
     gene            2106..>2445
                     /locus_tag="PK898_10410"
     CDS             2106..>2445
                     /locus_tag="PK898_10410"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU20479.1"
                     /translation="MGKIIPELFREIIQNASIKFQTPAISVAVIEDGEISFAEGFGSR
                     TFGKNEEVTADTSYAIASMSKSTVSTSLAILREQNLFGWDDKVTKFLPDFRMADLFAG
                     QEMTVLDLLIH"
BASE COUNT          684 a          571 c          556 g          634 t
ORIGIN      
        1 gagcatgata aacttacctg aagagttctt ctatgatttt gcaaagcctt tctttaacga
       61 atttccgctc ctatgttcga ttggatgtcg aatttcctca aggtattacg attctttccg
      121 gcggaaatgc acagggaaaa acaagtttgc tggaaagcct tttttactgt gccacttttg
      181 ctacgatatt agccggaaac gatcgccaac tgattaattt tgaagcgcaa aagcaggatc
      241 tggcggtcgc acgcatcgtt gtccgatact cgcgcgatca gcaaaatcat cagctcgaag
      301 tgagaatcat tcaggaaagc caaaatcgaa ttactcggac gcgcaaagag attctctgga
      361 acggacaaaa gctgcgtgcc caaaatgcga tcggtaaatt ccccgccgta ttatttatcc
      421 ctcagatgac tgccattttg gaggggcctc cgcaagaaag acgccgatat ttaaatatct
      481 tgctttcaca atccgaaccg ggttacgccc agactttaac cgagtacaaa caagctttgg
      541 ttcagcgcaa cgctttatta cgccaaatag ccgacaaaaa gtccgatccg gcgcagttgg
      601 aatattggga tgaattgctt tcgagtcgcg gagcggtttt gattcaaaaa agaataaaag
      661 cgctcgagac tttgaatgag tatgcggaag aaattcattt gcagttgacc gatcggcagg
      721 aaaaattagc cctgatctat aacccatcct ttgatccctt aaaccccgac cgttcaaatc
      781 ccgttgaaga aaattcggga cgccgcgacg gtttttcggc ggatgaaatt gcttcggcaa
      841 tgaaagctca tctgaaaacg attcgccggc gcgaaatcga gcgcggaact tcggttatcg
      901 gaccccatcg ggatgatttt caagtttgcg ccaatgatat cgacctgagt ctgtacggat
      961 cccgaggtca aatccgcacg gcgttgcttt cgctcaaaat ggcagaaata cgatggatcc
     1021 aagataaaac cggtacggag ccgttgctgc tgttggatga gacactcgcg gaattggatg
     1081 agaagagacg acgggattta ctggaaagat tggatcaata caaccaagga attctaacga
     1141 cgacagacct cacccatttt tcggatgatt ttttgaaaaa acataccgtt tggcagatag
     1201 ccaacggtgt tgtgcaaaaa gattaatcga tcggtttttt acgaatctga tccgggcgac
     1261 gcgggtatcc cgatggagta tttctttgtt tttcgatccg gataagatac cgcggttcgg
     1321 ctgcatttgg gatggtcacc ggaatgagct gtgtttcacc ccctcccaga atccggatgg
     1381 ccttttcggc tgctttcaat tcttccgccg cggtctcccc tttttgtgcg atcattatcc
     1441 ctttcctttt taccaaaggt aacaatagtt ccgaaagggc agataacccg gttaccgctc
     1501 tggcaacggc gatatcatac cgttcccgat ggagcggatc ctttccgaga tcttccgcac
     1561 gggcatgaat aatccggcaa tccgtcagag acagccgatc gataatatgc tgacaaaata
     1621 ccaatttttt ccccacggaa tccaccaacg tcaactttag atcgggaaaa acgattttca
     1681 acggaattcc ggggaatccg gcgcccgtcc ccacatcaac cagcgatcga ccgttaaaat
     1741 cgttgatcgc caatacacag gataacgaat ccaaaaaatg ttttgtccga atttcatccg
     1801 ggtttttaat ggcggttaaa ttgaaacgtg tattccactc gatcagttcg gattcataaa
     1861 tttcaaataa tcgaagccga tcctcggata acggaaatcc tataaattgt gcggcgatat
     1921 caatccattc cgacatatgt tgcattatag tagaattccg tttttcgagc cgaatttcgg
     1981 ccggaaaata ttccgattcg ataatcgttt cgaaatatca ccggcgaaga gagatcagcg
     2041 gctatccgat cgaaaatcat agtatagtaa gagctgacat ttaccttcac agaaagccgt
     2101 caaccatggg aaaaattatt ccggaattat tcagagagat tattcaaaat gcgagcatca
     2161 aatttcagac gccggcgata tcggtggctg taattgaaga cggagaaatc agtttcgcag
     2221 agggtttcgg gagccgaaca ttcggaaaaa acgaggaagt caccgccgat acctcttatg
     2281 ccattgcatc catgtccaag tcaactgttt ccacctcctt ggcgatatta cgggaacaaa
     2341 atttgttcgg ctgggatgat aaagtcacca aattcctgcc ggattttcgt atggctgatc
     2401 tttttgccgg gcaggaaatg accgtccttg acttgttaat ccatc
//
LOCUS       DAOSMM010000140         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_43386_length_2427_cov_14.298061, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000140 DAOSMM010000000
VERSION     DAOSMM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43386_length_2427_cov_14.298061"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PK898_10415"
     CDS             complement(<1..1037)
                     /locus_tag="PK898_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20480.1"
                     /translation="MTVDLGNALKNSNNAGNPITLKSESLGKDAFRRLIRNRAALVGG
                     IIIILLYVIMIFADKIAPFDFAQQTLSEQNAVPPWLMKVFPGMQAYGKPPVDNPNKYL
                     LGADYVGRDILSRIIYGSRVSLIIAIIGPLISLVIGIIYGSISGFFGGRVDNIMMRIV
                     DVLYAFPSLLFTILLMTFFRNTLSNPAPGTFGAALSALDSNLGGMLFIFIGIGLTAWE
                     TMARLTRGQVLSIKEKEFVEAARAIGSGNGRIMFRHIMPNIIGPLVVNETLAIPGYIN
                     TEAFLSFIGLGVNPPTPSWGAMISDGSKVVRTYPNQTIFPALMLAITMFAFNFLGDGL
                     RDALDPQMRGRT"
     gene            complement(1071..2126)
                     /locus_tag="PK898_10420"
     CDS             complement(1071..2126)
                     /locus_tag="PK898_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20481.1"
                     /translation="MGRYILKRLLYMLLVLFVVSAITFGLMHSVPGGPFDKEKPLPKE
                     IIANLEKRYHLDQPLPVQYLEYVKALIIPEVSTKKFPNTIDDDALFKFKIGNYYVKWV
                     NFGPSYSSRSRTVNDMLKDQLPISIQLGFLAFCVAVIIGMPLGIVAALKQNTFIDYLC
                     MTLAIFGVSVPVIVLGPILIWIFGVSLKWLPVTGWGSKPPFVGGFLPQTWGWQYWKYA
                     IMPMFALGLSSSASIARLTRASLLQVIREDYIRTARAKGLKERAIISGHALKNALIPV
                     VTILGPMFAALLTGTFVTETIFGIPGMGRYFVNSITARDYPVIMGTVLIYAVFLVVCN
                     LIVDLVYGLLDPRIRFE"
BASE COUNT          735 a          605 c          465 g          622 t
ORIGIN      
        1 gttcttcctc tcatttgcgg atcgagcgca tcccgaagtc cgtctccgag gaagttgaat
       61 gcgaacatcg taatcgccaa catgagtgcc ggaaagatcg tttggttcgg gtaagtccgg
      121 acaactttgg aaccgtccga aatcatcgca ccccaggacg gagtcggagg attcactccc
      181 aagccgataa atgaaaggaa tgcttccgta ttgatatacc ccggaatcgc cagcgtctca
      241 ttcacaacta acggtccgat aatattcggc atgatatgtc gaaacataat acgtccgttt
      301 ccgcttccga tcgcccgagc cgcttcaaca aattcctttt ctttaatcga cagcacctga
      361 ccacgagtca aacgagccat cgtttcccaa gccgtcagtc cgatgccgat aaaaataaat
      421 aacattcccc ctaaattgct gtccaacgcg gaaagggcgg ctccaaaggt tcccggagcc
      481 ggatttgaaa gagtattgcg aaagaaagtc atcaacaaga tcgtaaacag caggctggga
      541 aaagcatata aaacatccac aatccgcatc ataatattgt caactctccc gccgaagaac
      601 cccgaaatac tgccgtatat aatgccgatc accaaactga ttaacggccc gatgatggca
      661 ataatcagcg atacccgtga tccgtagata attcggctca aaatatctct tcccacataa
      721 tcggcaccca acaaatattt attcggatta tccaccggcg gttttccgta tgcctgcatg
      781 ccgggaaaca ccttcatcag ccatggcggt acggcgttct gctccgaaag cgtctgttgg
      841 gcaaaatcaa acggggctat tttatcggca aaaatcataa tcacatacag caaaatgatg
      901 atgattccgc cgaccaaagc ggcccggttt cgaatcaatc gtcgaaaagc atccttcccc
      961 agtgattccg atttcaatgt aatcgggttg ccggcattgt tcgaattttt caaggcattt
     1021 cctaaatcaa cagtcattca ttcctcctga aatttttctc acagccaaat tcattcaaat
     1081 cgaatccggg gatccaacaa accataaacc aaatcgacaa tcaggttaca aacaaccaag
     1141 aacacggcat agatcaggac agttcccata atcacagggt aatcccgtgc ggtgatactg
     1201 tttacaaaat atcgtcccat accgggaatg ccgaatattg tctccgtgac aaacgtgccg
     1261 gtcaacaatg cggcaaacat cggacccaaa atagtcacaa ccggaatcaa tgcatttttc
     1321 aacgcatgtc cggagatgat ggcacgttct ttcaaccctt tagcgcgggc ggtccgaata
     1381 taatcctcgc ggatcacttg cagtaaactg gcgcgagtca aacgggcaat ggatgcggag
     1441 gaagagagtc ccaaagcgaa catcggcata atggcatatt tccaatactg ccatccccaa
     1501 gtttgcggta aaaatccgcc aacaaacgga ggcttcgacc cccaaccggt taccggcaac
     1561 catttcaagg aaaccccaaa aatccaaatc aaaatcggtc ccaagacaat gaccggcacg
     1621 gataccccga atatcgccaa cgtcatacat aaataatcga taaatgtatt ttgttttaat
     1681 gcggcgacaa tcccgagagg catcccgata attaccgcca cgcaaaatgc caaaaatccc
     1741 agttggatcg aaatcgggag ctgatctttg agcatatcat taaccgttcg ggatcgtgaa
     1801 gaataggatg ggccgaaatt cacccatttt acataatagt taccgatttt aaatttaaac
     1861 agggcatcat catcaattgt gttcggaaat tttttcgtac ttacttccgg aataattaat
     1921 gccttcacat attccaaata ttgaaccggc aatggttgat ccaggtgata acgtttttcc
     1981 aaattggcaa taatttcctt tggcaacggc ttttctttat cgaaaggtcc tcccggcaca
     2041 gaatgcatca aaccgaatgt gatggccgac accacaaaaa gtaccaataa catataaaga
     2101 agccttttca ggatatatct acccatttct tttcctccgg aattaacaga taccttcgat
     2161 caaacagttc atcgataaat cctgtcaact cctgttcctt gttattttat attgtttttg
     2221 tcatccattt aattattcag gtcgatatga tgatgaaaat ttcattatca tatcgatttg
     2281 aataagttat tttgattttt ttctctttcc acatctctta gatggttatt tcttcgttaa
     2341 aagaggaatt ttcccattta ttgagggggc tataaattat agccccctct tgatcactgt
     2401 aaaatcacat tggataacag atcagat
//
LOCUS       DAOSMM010000141         2307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_46534_length_2307_cov_9.539520, whole genome shotgun sequence.
ACCESSION   DAOSMM010000141 DAOSMM010000000
VERSION     DAOSMM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46534_length_2307_cov_9.539520"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..497
                     /locus_tag="PK898_10425"
                     /pseudo
     CDS             54..497
                     /locus_tag="PK898_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            645..926
                     /locus_tag="PK898_10430"
     CDS             645..926
                     /locus_tag="PK898_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20482.1"
                     /translation="MYAGRIVEKGSAHDIFLNPAHPYTVGLMASKPVVNKKVDRLYSI
                     PGSVPSPIHMPNYCYFRDRCDKRFGRCNDDYPPEFCLSETHRTNCYLYE"
     gene            1032..2000
                     /locus_tag="PK898_10435"
     CDS             1032..2000
                     /locus_tag="PK898_10435"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOU20483.1"
                     /translation="MNDAAFFEVKNLKKYYPIKSGFFNKVSGFVKAVDGISFTIEKGK
                     TMGLVGESGCGKTTVGKTILRLNSKTAGDVLYKGRELFELDAKEMRKLRPKMQIIFQD
                     PYSSLSPRLPVGEIIGEAVREHKIVPEDEFDDYITRIMIACGLQEYHKDRYPHEFSGG
                     QRQRICIARALALSPEFVVCDEPVSALDVSIQAQIINLLEDLQHQFNLAYLFISHDLS
                     VVQHISDTVAVMYLGNIVEFSDRDEIFSNPLHPYTKALFSAIPIPDPTIRRNRIILQG
                     SIPSPANPPKGCKFHTRCAYAMKKCETVEPVLRDLGNGHTVSCHLF"
     gene            2011..2289
                     /locus_tag="PK898_10440"
     CDS             2011..2289
                     /locus_tag="PK898_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20484.1"
                     /translation="MKNRIQNRDEIDQDDGRTVINMNVDGMPWFRDQNTEISQVPSTE
                     NQIDSHHQMGWKESFIISKYVLKASLWIIACYSIGIILVILFLIKIWS"
BASE COUNT          694 a          431 c          510 g          672 t
ORIGIN      
        1 ggaacggtaa aagcggttga cggggtttct tttgaagttc cgaaagggaa acagtcggaa
       61 tcgtcggaga gtcgggatgc ggaaaatcaa tcaccagcct ttcattgatg caattggtgc
      121 aacgcccgca ggggcaaatc gttcagggtg aaattcgctt taatttaggc gaaaaatctt
      181 atgacatcgc aaaaatgccg accgaagcaa tgcaaaaaat tcgcggcaat caggtatcga
      241 tgatctttca agagccgatg accagtctga atccggtttt tcgggtcggc gaacaaatag
      301 atgaggtaat taaattacat tatccgcaga tgcaaaaaga cgagatcaag aatcgatcac
      361 taaaaatgtt ggacttagtc ggcatagcaa atggggaagg tgtttacaaa atgtatccgc
      421 acgagctttc cggcgggatg agacaaagaa ttatgattgc gatggccgtc gcttgtgatc
      481 caaaattaat tattgcggat gaaccgacta ctgctttaga tgtaaccatt caggcacaaa
      541 ttttagatct gtttcggaat ttaaaagaga agatcaacag ttcgattatg ttaatcactc
      601 acgatctcgg agtcattgcc gaaatggcag attttgtcgt agtgatgtat gcgggtagaa
      661 ttgtggagaa agggagtgct cacgatattt ttttaaatcc cgctcatcct tataccgtcg
      721 ggttaatggc ttccaaaccg gttgttaata aaaaagtaga ccgcctttac agtattccgg
      781 gttcggtccc cagtccgatt catatgccga attactgtta tttcagagat cgatgcgata
      841 agcgattcgg ccgttgtaac gacgattatc ctcccgaatt ctgcctttcc gaaacacata
      901 ggacgaattg ttatctttat gaatgatctt ttgataattc aaataatttt ttcgaaccgc
      961 gaatttcctt ttttttatat ttcttcattt tgttcaaaat atcaatcaga aagagaagga
     1021 aatcaacttc tatgaacgat gccgcttttt tcgaagtaaa aaatttaaaa aaatattacc
     1081 cgatcaaatc cggtttcttt aacaaggtaa gcggttttgt aaaagcggtt gacgggatta
     1141 gtttcacaat tgaaaaaggg aagacgatgg gtttggtcgg agaatcaggg tgcggaaaaa
     1201 ccacggtcgg gaaaactatt ttgcgcttaa acagtaaaac cgcgggagac gtcctttata
     1261 aaggacggga attatttgaa ctggatgcga aagaaatgag aaaactgcga cccaaaatgc
     1321 aaattatttt tcaagatcca tattcaagcc tttctccacg cctccctgtc ggagaaatta
     1381 tcggagaagc ggtcagagag cacaaaatcg ttccggaaga tgaatttgac gattatatta
     1441 caaggataat gattgcttgc ggtttacagg aatatcataa agatcgttat ccgcatgaat
     1501 tttccggcgg ccagcgccag cgtatttgta ttgcccgtgc cttagcgctg agcccggaat
     1561 ttgttgtttg tgatgaaccg gtatcggctt tggatgtgtc tattcaagct cagattatca
     1621 acctgctcga ggatttacaa catcaattta atttggcata tttgttcatt tctcatgact
     1681 tatctgtcgt acaacatatt tccgatacgg ttgcagtaat gtatttggga aatattgtgg
     1741 aattctccga tcgagatgaa atattttcaa atccgttgca tccctatacg aaagcattat
     1801 tcagtgccat cccgattccg gatccgacga tcagacggaa tcgtattatt cttcaaggga
     1861 gtattccttc tccggcaaat cccccaaagg ggtgcaaatt tcatacacgt tgcgcatatg
     1921 ccatgaaaaa atgtgaaacg gttgaaccgg tcttaagaga tttgggcaac ggacatacgg
     1981 tgtcttgtca tttgttttga tggagttgct gtgaaaaatc ggatacaaaa tcgtgacgaa
     2041 atagatcaag atgacggcag aaccgttatt aatatgaatg ttgatggaat gccttggttt
     2101 cgagatcaaa atacggaaat cagccaagtc ccttccacag aaaatcaaat cgactcccat
     2161 catcaaatgg gatggaaaga gtcttttata atatcaaaat atgtcctgaa agcaagttta
     2221 tggatcattg cttgctattc aatcggtatc attctggtta ttttattctt aattaagatc
     2281 tggtcttaac ttgatccgtt ttcttct
//
LOCUS       DAOSMM010000142         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_46618_length_2304_cov_16.748333, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000142 DAOSMM010000000
VERSION     DAOSMM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46618_length_2304_cov_16.748333"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1339)
                     /locus_tag="PK898_10445"
     CDS             complement(8..1339)
                     /locus_tag="PK898_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU20485.1"
                     /translation="MIAASICQDGSSKKKILISSLLFMGLGHILYLKQYVKGILYALI
                     EILFLFFTPTVYKKIAGLITLGDPHPELPIKQRDHSMFMMIDGILILAVVFIFILLYY
                     LSVQNALSDYKDYCATGIKAKNKNILAELGNKSFPIVGLMPMILLVLFFVVVPLIFSA
                     CVAFTNYSAPKNIPPNNTVDWVGLDNFRALFGGDATWSGALGRVAGWTLIWAFFATLT
                     CYAGGMVMAVVLQESKIKIKPFFRSIFILPYAVPTVVSMLVWQNLLNGSFGTVNRTLI
                     ALGLLDKSAVIPWLSNVTLARFVVILINLWAGFPYFMLLTMGTMTSISNEQYEAAGMD
                     GCTKTQAFRYITLPQVLYQTMPLIIMSFTYNINNFGAIFFLTGGNPIVADSTVTSARG
                     TDIMVTWIYKLTITLLKYNYASVLAVCIFVVLAPFAIYNFRRTKSYKEGEL"
     gene            complement(1492..>2304)
                     /locus_tag="PK898_10450"
     CDS             complement(1492..>2304)
                     /locus_tag="PK898_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU20486.1"
                     /translation="VMYGYPVSAETYALYYNKDLIPAEEIPTTWEELKTWTAAFNAKN
                     PNKYGFLMDVGNIYYTILFTTMNGNRLFGPDGTDTSSSYLATEDAIAGMKIFQGLHEV
                     VPLAAADANTATADGAFAAGNVAMYISGPWNVANFTNAGIHFGVTTLPSLDGTTPAAS
                     FSGTRGMFVSAYSEHPEEAAKFAEFLISPEMQKLRYDITGALPSIPLEVESEAAKGFI
                     AQLEYAFPMPSVPQMGAFWESGNAASANIWDGADVETELKALDKAIVSFVAQ"
BASE COUNT          674 a          529 c          544 g          557 t
ORIGIN      
        1 tctaccatca cagctctcct tccttgtatg attttgtcct tctgaagtta taaatcgcaa
       61 aaggagcgag aactacaaaa atgcataccg ccaacacgga ggcatagttg tatttcaata
      121 atgtaatagt caatttatag atccaggtca ccataatatc cgtgccgcgg gcgcttgtga
      181 cagtggaatc ggctacgatc gggtttccgc cggtcaggaa gaaaatggca ccaaaattgt
      241 tgatattgta ggtgaatgac ataataatca gcggcatggt ttgatacaaa acctggggca
      301 gcgtaatata gcgaaaagct tgcgttttgg tgcagccgtc catgccggcc gcttcatatt
      361 gttcgttact gattgaagtc atcgttccca tcgtcagcaa cataaaatac gggaaacccg
      421 cccaaagatt gatcaaaatg acgacaaagc gagcgagggt tacgttgctc agccatggga
      481 tgaccgccga tttatccaga agtcccaatg cgattaaagt acgatttacc gtcccaaacg
      541 aaccgttcaa caggttttgc cagaccaaca tgctgacaac cgtcggaacc gcgtaaggca
      601 agataaaaat ggaacgaaag aagggtttga ttttaatctt tgactcctgt aaaaccaccg
      661 ccataaccat tccgccggcg tagcaggtca acgttgcaaa gaaagcccag attaatgtcc
      721 aaccggcaac ccggccgagc gctcccgacc aggtggcatc tccgccgaag agcgcacgga
      781 aattatcgag gcctacccaa tcgacggtat tgttgggcgg aatgtttttc ggcgccgaat
      841 aatttgtaaa cgctacgcaa gctgaaaaga tcaacgggac gacgacaaag aataatacga
      901 gcagaatcat gggcatcaag ccgacgatcg gaaaagattt atttcccaat tcggcaagga
      961 tattcttatt ttttgcttta atgccggtag cgcaataatc tttgtaatcg gataatgcat
     1021 tttgaacgga caaataatac agcagaataa aaataaatac gaccgccagg atcagaatgc
     1081 cgtcgatcat cataaacatg gaatgatcgc gctgtttaat gggtaattcc gggtgaggat
     1141 ctcccagcgt gattaagccg gcaatctttt tatatacggt cggagtaaaa aacaaaaaaa
     1201 gaatttctat aagtgcatag agaattcctt tgacgtattg cttcaaataa agaatatgtc
     1261 ccaatcccat aaaaaggagg gacgaaatca agatcttttt tttcgacgat ccatcctgac
     1321 agattgaggc ggctatcatg ataattcctt tccgacgaca gagaagcaaa gaactcaaac
     1381 aggatcaaaa aagaaatcga cgaagcaacc gataccgcta taaaaattat tgaaaaaaga
     1441 agctcccttt cagagcgaac aacgaaaggg agctttgtac tcatcagatc tttattgtgc
     1501 tacaaacgat acgattgctt tgtccagcgc tttcagttcg gtttccacat cggcgccgtc
     1561 ccaaatattg gcggatgccg cgttaccgga ttcccaaaaa gcgcccattt gcggtacgga
     1621 tggcatcggg aatgcatatt ccagttgagc gatgaatcct ttggcagctt cactttcgac
     1681 ttccagcgga attgacggta acgcaccggt gatgtcatag cgcagtttct gcatttcggg
     1741 tgaaatcaaa aattcggcaa atttggctgc ttcttccgga tgttccgaat aggcggaaac
     1801 aaacattcca cgcgttccgg agaaagatgc cgccggagtt gtaccgtcca acgaaggcaa
     1861 cgtcgtaacg ccgaagtgaa tccccgcatt ggtgaaattg gctacattcc aggggccgga
     1921 aatgtacatt gccacgtttc ccgcggcaaa tgccccatcg gcagtggctg tgttggcatc
     1981 ggcagccgcc aacggaacga cttcatgcaa accctggaag attttcatgc cggcaatggc
     2041 atcttccgtt gccagatagg aggaagatgt gtccgtaccg tcgggaccga ataagcggtt
     2101 tccgttcatg gtggtaaaca gaatggtgta ataaatgttt cctacgtcca tcaggaaacc
     2161 gtatttgttc gggtttttgg cgttgaaggc cgcggtccac gtcttcaatt cttcccaagt
     2221 ggtcgggatt tcttctgcgg gaatcagatc tttgttatag taaagcgcat aggtttcagc
     2281 cgaaaccgga taaccgtaca tgac
//
LOCUS       DAOSMM010000143         2289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_47035_length_2289_cov_16.300358, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000143 DAOSMM010000000
VERSION     DAOSMM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47035_length_2289_cov_16.300358"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..884
                     /locus_tag="PK898_10455"
     CDS             150..884
                     /locus_tag="PK898_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOU20487.1"
                     /translation="MEIYAHRGYSIAYPENTLLAFNKAYEIGANGVELDVRLTKDGEV
                     IVMHDDTVDRTTNGTGKVSELTLKEISKLDAGSWKGIAFENEPVPTLKKVLESLGHKT
                     KLIIEFKNDKEQNPDYLVDQVVALIEETETMKNVIVSSFEFKNLVTTKSRMPDLSTAL
                     ILEKGISGFWDRHFFKHSINVDALHPSIGEVTEKLVRTEHLNHRKVRVWGVNESFDMM
                     QMEKFGVDAIITDDPKLGLIYNLNQP"
     gene            complement(949..>2289)
                     /locus_tag="PK898_10460"
     CDS             complement(949..>2289)
                     /locus_tag="PK898_10460"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOU20488.1"
                     /translation="RKGAIMAQTLTTCPRCQHQMTANIQQIFDLHEDPSDKEKLLTGQ
                     VNMAVCPSCGYQSAIGVPLVYHDPEKELLLTYFPSELGLPINEQEKILGPLINKVVER
                     LPLEERKGYLLRPQAMLTYDTLIEKILEADGITKEMIQNEEKKVNLLRQLLTCSEESI
                     PEIIKQEEAQIDQSFFTLFTNIQASAVQSADDATRKRIKAIQDVLLTETAYGRELKKK
                     AESTQKAIKDLQDLGDKLNRDTLLELVLNSPDETYLQTLAGLARDGMDYQFFTNLSGR
                     IEKASSDEDKKKYTEIREKLLEMVQQIDAAIAGQKQLRKQVFDEIMKEEDLEQGIARY
                     ARAIDDSFLEIAQSELAAARKNGDFIRSGKIQKLIDTIEKMSEMPPEVEFLEELLQKT
                     DVAELTATLDANKEKLTGDFKKILDSLIAQLQQTPDQQQELLEKLKLIQKIINL"
BASE COUNT          610 a          512 c          497 g          670 t
ORIGIN      
        1 cgtatctcct atgaaaaaag gtataatttt gaataagtca atgagagaca ttgacataac
       61 gatttcggta attcacggaa aaaggctgat cagcccggaa tttgaatcaa tatcggggca
      121 aagtcaatac aagaaacaag gacggtccaa tggaaatata tgctcatcgg ggatattcca
      181 tagcgtatcc ggaaaacacg ctgcttgcat ttaacaaagc ctatgaaatc ggagcaaacg
      241 gggtggaact ggatgttcgc ctaacgaagg atggagaggt catcgtcatg catgacgaca
      301 ccgtcgatcg cacaaccaac ggcaccggaa aagtcagcga gctgacgctg aaggaaatat
      361 ccaaattaga tgccggatcc tggaaaggga tcgcctttga aaatgaaccg gtcccgactc
      421 tgaaaaaggt cctcgaatct ctcggccata aaacaaaact gattattgag tttaaaaatg
      481 ataaggagca gaatccggat tatttggttg atcaggtcgt cgcgttgatt gaggaaacgg
      541 aaacgatgaa aaacgtcatc gtttcttctt tcgaattcaa gaatctcgtc accacaaaga
      601 gccgaatgcc ggatttatcc acagccctga ttctggaaaa ggggatcagt ggtttctggg
      661 accgtcattt tttcaaacat tccatcaacg ttgatgcttt acatccttca atcggcgaag
      721 taaccgaaaa attggtccgt accgaacatc tgaatcatcg caaggtccgc gtgtggggag
      781 tgaatgagtc ctttgatatg atgcagatgg aaaaattcgg ggtcgatgcc atcatcaccg
      841 atgatcccaa attaggattg atttacaatc tcaatcagcc ttgattcaga aagctttcct
      901 ttaagcaaaa cagggaagat gccttccctg ttttttcttt caacgatatc acagattaat
      961 tattttttgt atcaatttca gtttctccag cagttcctgc tgttgatccg gagtctgctg
     1021 caactgcgca attaatgaat ccagaatttt cttgaaatcg ccggtcaatt tttctttatt
     1081 ggcatcgagc gtcgcagtca attccgccac atcggttttt tgaagcaatt cttccaaaaa
     1141 ttcgacttcg ggaggcattt cactcatttt ttcgatcgtg tcaatcagtt tctgaatctt
     1201 cccgcttcga ataaaatcgc cgtttttccg agcggcagcc aattcacttt gcgcgatctc
     1261 caagaaggaa tcatcaattg cacgggcata tcgggcgatc ccctgctcaa gatcttcctc
     1321 tttcatgatt tcgtcgaata cctgtttgcg cagctgtttt tgaccggcaa tcgctgcgtc
     1381 aatctgttga accatctcca gtaatttctc tcgaatttcg gtatatttct tcttgtcttc
     1441 atcggaagaa gctttttcga tccgaccgga taaattggtg aagaattgat aatccatccc
     1501 atcgcgggcc aaacctgcca atgtttgcag ataggtttca tccggcgagt taagcaccag
     1561 ttcgagcagc gtatcccgat ttagtttatc gcccaaatcc tgaagatctt tgatagcttt
     1621 ctgcgtgctt tccgccttct ttttcaattc tcgaccgtag gctgtctccg tcaacaagac
     1681 atcctgtatg gctttgattc gtttacgggt tgcatcatcg gcggattgaa cggcgcttgc
     1741 ttgaatattt gtaaaaagtg taaagaaaga ttggtcaatc tgcgcctctt cctgtttgat
     1801 gatttcggga atggattctt cggagcaggt taataattgc cttaataggt taactttttt
     1861 ctcctcgttt tgaatcatct ctttggtaat gccgtccgct tcgaggattt tttcgatcaa
     1921 cgtatcatag gtaagcatcg cctgcggtcg cagcagataa ccttttctct cttccaacgg
     1981 taagcgctcc actactttgt taatcaacgg gcccaaaatt ttctcttgtt cgttgatcgg
     2041 cagcccgagt tcggaaggaa agtaggtcaa aagtaattct ttttcgggat catgataaac
     2101 caaagggacg ccgattgcac tctgatagcc gcaagacggg caaacagcca tatttacctg
     2161 gcctgtcaga agtttttcct tgtccgaggg atcttcatga agatcaaaaa tctgttgaat
     2221 attcgctgtc atttgatgct gacagcgtgg gcatgttgtc aaagtttgag ccataatagc
     2281 tcccttcct
//
LOCUS       DAOSMM010000144         2262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_47808_length_2262_cov_7.166289, whole genome shotgun sequence.
ACCESSION   DAOSMM010000144 DAOSMM010000000
VERSION     DAOSMM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47808_length_2262_cov_7.166289"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2262)
                     /gene="ileS"
                     /locus_tag="PK898_10465"
     CDS             complement(<1..>2262)
                     /gene="ileS"
                     /locus_tag="PK898_10465"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOU20489.1"
                     /translation="WQILKNFFERDLLYQGFKVVPYCPRCGTPLADHEVAQGYHDVED
                     PSIYIRFRLKNQPDTDLLVWTTTPWTLTANVGIAAHPDVDYVKIERPLPDGSSEKLIF
                     AESLVPIVLPGEEIKILERYKGKDLKGWEYEPLFHFLPLDKPAHRVVMADYVSVEDGT
                     GLVHIAPSFGAEDMEVGKVEDLPVLMTVGPDGRFIPEVTDWAGMFVKDADPLIIKELS
                     ARGRMLKHGKIKHSYPFCWRCNTPLLYYARPTWFIRTSQFKDRLVELNERINWYPSHI
                     KYGRFGNWLENNIDWALGRNRYWGTPLPVWICDQCGHQECIGNREELSQKAGKDLSNL
                     ELHRPYVDEITYSCPECGGVMHRVPELIDVWFDSGAMPYAQWGYPNKNHEIFKKQFPA
                     DFISEAIDQTRGWFYSLHAISTLYMDQECFKNVICIGLILDENGHKMSKHIGNVVSPW
                     DVIKDNGVDAMRWYLFTSSPAGQERRFSSNLVNEVLRTFTLQLWNTYSFFVLYANLDQ
                     WKPEEGQNLEFNDLDRWLLSALNALVKEITAAYENYDVNAATRPIQTFVDRLSNWYLR
                     RSRRRFWKSGEDADKNAAYYTLYTALVTVAKLIAPAMPFIADELYRNLVCSVDPNAME
                     SVHMADWPKADETMIDEPLNHEMDLVINLASLGHSARNKANRKVRQPLSECAFSLGIA
                     ENQAVVEKYADVLKDELNVKKVRLLDAASEAMEFTLNPQPKQLGQKYGSLFPAIRKKI
                     LEMDSMTTGRQLL"
BASE COUNT          628 a          573 c          455 g          606 t
ORIGIN      
        1 ccagcaactg gcgaccggtc gtcatcgaat ccatttccaa aattttcttt cggatagcgg
       61 gaaataaact accatacttc tgtcccaatt gcttcggttg tggattcagc gtaaattcca
      121 ttgcctcgga tgcagcatcc aacaagcgga cttttttaac attcaactca tccttcaaca
      181 catcggcata tttttcgaca acagcttgat tttccgctat acctagagag aatgcacatt
      241 cacttaatgg ctgacgaact ttccgattgg ctttatttct tgcggaatgt cccagtgatg
      301 cgagattaat gaccagatcc atctcatgat ttaatggctc gtcaatcatt gtctcatccg
      361 cctttggcca atcagccata tgaacggatt ccattgcatt cggatccacg gaacaaacta
      421 aatttcgata gagttcatca gctataaacg gcatggcggg agcgatcaac tttgccacgg
      481 taaccaaagc cgtgtaaagc gtatagtagg cggcattttt atccgcatcc tcaccgctct
      541 tccagaatcg tcttctggaa cgacgcaagt accagttgga aaggcgatcg acaaatgttt
      601 gaatcggtcg ggtcgcggca ttgacgtcat agttctcata agcagcggta atttctttta
      661 ccaatgcatt caaggcagat aacaaccatc tatccagatc gttgaattca agattctgtc
      721 cttcttctgg cttccactga tccaaattgg catacagcac aaaaaatgaa taggtattcc
      781 acaattgaag tgtaaaggtc cgtaagactt cattaaccaa attggaagaa aaacgccgtt
      841 cctgacctgc gggactggat gtaaacaaat accatcgcat ggcatcaacg ccgttatctt
      901 taataacatc ccacggggaa acaacattcc cgatatgttt ggacatttta tgaccgttct
      961 catccagaat cagtccgata cagatcacat tcttgaaaca ttcctgatcc atataaagcg
     1021 tactgatcgc atgtagcgaa taaaaccaac cacgtgtctg atcaatcgct tcgctgatga
     1081 aatcggcggg gaactgtttc ttgaaaatct catggttctt attcgggtag ccccattgag
     1141 catagggcat cgcacccgaa tcaaaccaga catcaatgag ttcaggcacc cgatgcatta
     1201 ctcccccaca ttcgggacaa gaatacgtaa tttcatcaac ataaggtcga tgcaactcta
     1261 agttcgaaag atctttaccg gctttttggg ataattcttc ccgatttccg atacattcct
     1321 gatggccgca ttgatcgcag atccaaaccg gtaagggtgt tccccaatat cggtttcttc
     1381 ccaatgccca atcgatatta ttttcaagcc aatttccaaa tcgtccgtac ttaatatgag
     1441 acggatacca gttaattctt tcgttcaatt caaccaaccg gtctttaaac tggctagtac
     1501 ggataaacca tgtcggtcgg gcataataaa gcaaaggagt gttgcagcgc cagcagaaag
     1561 gataactgtg tttgattttg ccgtgtttca gcattcgtcc gcgagcgctt agttctttga
     1621 taatcagcgg atccgcatct tttacgaaca ttcccgccca atcggttact tccgggataa
     1681 accgcccgtc tggtccgacc gtcatcaaaa ccggtaaatc ttccactttg ccgacttcca
     1741 tatcttccgc gccaaatgaa ggggcgatat ggaccagtcc cgttccatct tccacagaga
     1801 catagtcagc catcaccacc cgatgggcag gcttatccag aggaagaaaa tggaaaagcg
     1861 gttcgtattc ccaacctttt aaatctttcc ctttatagcg ttccagaatc ttgatctctt
     1921 cccccggcaa gacaatggga accagcgatt cggcaaaaat caacttttca gaacttccgt
     1981 cgggtaaagg ccgctcgatt ttgacataat cgacatccgg atgggcggca attccaacat
     2041 tcgctgtcag cgtccatgga gtggtcgtcc aaaccagcaa atcagtatcc ggctggttct
     2101 ttaagcgaaa tcgaatatag attgaaggat cttccacatc atggtagccc tgtgcgactt
     2161 catgatccgc caggggagtt ccgcagcgcg ggcaataagg aacaacctta aacccctgat
     2221 aaagcaaatc tctttcaaag aaattcttca ggatttgcca tt
//
LOCUS       DAOSMM010000145         2238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_48477_length_2238_cov_13.716445, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000145 DAOSMM010000000
VERSION     DAOSMM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48477_length_2238_cov_13.716445"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK898_10470"
     CDS             complement(<1..442)
                     /locus_tag="PK898_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU20490.1"
                     /translation="MKKGLSIVLSLFMIASLFIVSVPVSAQDAVKLVVWESTGGPDEW
                     IKAAGEAFTKLNPNITVEFVNVELGDTSGQIALDGPAGVGPDLFAAPHDKLGELVVGG
                     HILPTVNPDAVKEAVLASTSQALTYDGVMYGYPVSAETYALYYNK"
     gene            complement(714..1145)
                     /locus_tag="PK898_10475"
     CDS             complement(714..1145)
                     /locus_tag="PK898_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU20491.1"
                     /translation="MKTVQFTLQTSGRSQLIDITSRVREAVTASGIREGLCTIFIPHT
                     TAAVTINENADPDVVRDFLYELDKIVPWQDGYRHAEGNSAAHLKSSLIGVSEQVLIEN
                     GRLMLGTWQGIYFCEFDGPRTRRVLVRIDSSDFSTGNTAAA"
     gene            complement(1219..>2238)
                     /locus_tag="PK898_10480"
     CDS             complement(1219..>2238)
                     /locus_tag="PK898_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOU20492.1"
                     /translation="RKAEEYGIPKEDVIIDCLTLTVSAQQEQAMETLKALRTVKERFG
                     VKTVLGVSNISFGLPDRETINAAFLTLALGQGLDLPIINPNVRTMTGAVDAFKVLAGQ
                     DKNSERFISIHNQIQSEKPIVPAGGSVSPDSSLEYAIANGLRQIAAEATVRLLNTMEP
                     AEIIDHILIPTLEKVGNRFEKGEIFLPQLIQSAGAAQAGFEEIRKAMTRSSQSEVNLS
                     KGKIILATVKGDIHDIGKNIVKVVLENSGYQIIDLGKDVPIEVVRQTVLRENAKLVGL
                     SALMTTTVKNMEETIRDLKNHSECKIMVGGAVLTKDYALSIGADYFAKDAKAAMDIAK
                     KVFEE"
BASE COUNT          558 a          557 c          515 g          608 t
ORIGIN      
        1 ctttgttata gtaaagcgca taggtttcag ccgaaaccgg ataaccgtac atgacgccgt
       61 cataagttag tgcctgagag gttgaagcga gaacggcttc cttaaccgca tccgggttta
      121 ccgtcggaag aatatgacct ccgacgacca gctctcccag tttatcatgc ggtgccgcga
      181 acagatccgg tccgactcct gccggcccat ccaaagcaat ctgaccggag gtatctccca
      241 attccacatt gacgaattca acggtaatat tgggatttaa tttggtgaac gcttctccgg
      301 cggctttaat ccattcatcc ggtccgccgg tggattccca aaccaccagt tttacggcat
      361 cctgagccga taccgggacg gaaacaataa aaagtgaagc aatcataaac agcgaaagta
      421 caatcgataa cccttttttc attttttccc ctttctgaat tcaaatgatt attccggaaa
      481 tattcgatct tgctccggtt aataaagtgt ggacagcgaa tccgaattga ccgttccggt
      541 aaaaactctt tgctattggc attttttgtt ttaaccgaat ccggctattt aaattatata
      601 tcgaataagt gatttatttt gatatctgtt tgtatattta aagaaggacg aatgatacat
      661 aaaaccgctc tgatcgatcc gctgaatccc gatatggcag gggacggagt gaattaagcg
      721 gcggctgtat ttccggttga aaaatcggat gaatcgatcc ggaccagtac ccggcgcgta
      781 cgcggtccgt cgaactcgca aaaataaatc ccctgccagg ttcccagcat taatcggccg
      841 ttttcgataa ggacctgttc ggatacaccg atcagcgagg atttcagatg ggcggcggaa
      901 ttcccttccg catgccggta cccgtcctgc cacggtacga tcttgtccaa ttcgtacagg
      961 aaatcacgga caacatccgg atcggcgttc tcatttatgg taacggcggc cgtggtatga
     1021 gggataaaaa tggtacagag tccctcgcgg ataccggaag ctgtgaccgc ttcccgaacc
     1081 cgagaggtga tatcgattaa ttgggaacgc ccggaagttt gaagtgtaaa ttgaacggtt
     1141 ttcacggaat aggctccttt tatcaaatat tcttaaaaat gaatcatatt cgggtctccg
     1201 gtacccgatc aaacggattt actcttcgaa gacttttttg gcgatatcca ttgccgcttt
     1261 ggcatccttt gcgaaatagt cggcaccgat tgacagcgca taatctttgg tcagcaccgc
     1321 cccccctacc atgatcttgc attccgaatg gtttttgaga tcccgaatcg tttcttccat
     1381 attctttacc gttgtggtca tcaatgcact cagcccgacc aattttgcgt tttcccgcag
     1441 aaccgtttgc ctgacaacct caatgggaac atctttcccc aggtcgataa tctgataccc
     1501 ggaattctca agaacgactt tgacgatgtt tttcccgata tcatgaatat ctcccttaac
     1561 cgtcgccaat atgatttttc ctttggaaag gttcacttcg gactgtgaag agcgggtcat
     1621 agctttgcga atttcttcga agcccgcttg agcggcaccg gcggattgaa tcagctgcgg
     1681 caaaaagatt tccccttttt cgaaacggtt ccctactttt tcgagggtcg ggatcagaat
     1741 atgatcgatt atttccgcag gctccattgt gttaagcaac cggacggtgg cttcggctgc
     1801 aatttgccgg agcccatttg caattgcata ttcaagggat gaatctggcg agacggaacc
     1861 accggctgga acgatcggtt tttcgctttg tatttgatta tggatggaaa taaatcgctc
     1921 ggaatttttg tcctgaccgg cgagcacttt aaacgcatcc accgcaccgg tcatcgtgcg
     1981 gacattcggg ttgatgatcg gcaaatccag accctgaccc aaagcgagag tcaagaaggc
     2041 ggcattaatc gtctcccgat ccggcagtcc gaaggatata ttcgaaactc ccaatacggt
     2101 ttttacacca aaacgttctt ttaccgttcg gagggctttc aacgtttcca tcgcctgttc
     2161 ctgctgagcc gaaacggtga gagtcaggca atcgataatg acatcttcct tcgggatgcc
     2221 gtactcttcg gctttacg
//
LOCUS       DAOSMM010000146         2176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_50387_length_2176_cov_17.754361, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000146 DAOSMM010000000
VERSION     DAOSMM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50387_length_2176_cov_17.754361"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PK898_10485"
     CDS             complement(<1..410)
                     /locus_tag="PK898_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20493.1"
                     /translation="MKSTHPLLNLKSYSSHFQKDWDPMKILSHSEVTFRKWQISSLFL
                     FVLIFLFGSNLPVFSQEADPVQEENISVSFECSYPVNVQKVAPKSEDSLSELSQKTER
                     NVDVVKSITITDSSGEETLTVWNYQLVNVNIVWDA"
     gene            complement(586..1305)
                     /locus_tag="PK898_10490"
     CDS             complement(586..1305)
                     /locus_tag="PK898_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20494.1"
                     /translation="MKRKLIVLILIAAVLAAFSPASQNFREPYRKSTPKSAPTKNVLP
                     PPLIKSTAIPDFTETPETLEPVQIPEATEIPKVPEILESTETPETMEILPAQLGQKIN
                     CGNKFQFTFYYQPLMSKSNSGQVAKGKFLVARVLITNLTDRTLEGLPARSFRLHGIID
                     GVDQEYKLHIANSFITSDRWDVGLITDTIQAGGMLDTFLVFDVPGKADNWYLKFEPIA
                     KSSEGPFCRVAIQVPKINYVD"
     gene            complement(1351..2088)
                     /locus_tag="PK898_10495"
     CDS             complement(1351..2088)
                     /locus_tag="PK898_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20495.1"
                     /translation="MKRHFWILSVILLLFMIFGASAQSEGDDQSRQTGDNLLKNSEKV
                     FPRLLINEDFSENLPLDVYEDESNGIKITSVIQQKNGVRKATNYVDGLIKDIEGLSAF
                     YSETYYGTLSEFHLHVEVSILNEFPDSTGSCFIEYTNEAVVGENSRQTVEIILGSRID
                     TYQTKSGSRVLTTLYDLSGTHEVGKIYNIDIIRYGGIGYFFIDGVFLIAYQDNIKENL
                     SWMTGPIVGIGGEQTTCIFDKIWVRRK"
BASE COUNT          628 a          430 c          414 g          704 t
ORIGIN      
        1 gcatcccaga cgatgtttac attgaccaat tgataattcc aaacagtcag tgtctcttct
       61 ccggaactgt cggtaattgt gatactcttg acgacatcta cgtttctttc tgttttttga
      121 gataattcgg aaagtgaatc ctctgacttc ggagcaactt tttggacgtt aaccggatag
      181 gagcattcga atgaaacgga aatattttct tcttgtacgg gatccgcttc ctgactgaat
      241 acgggaagat ttgagccgaa gagaaaaata agaacgaata agaataaaga ggaaatttgc
      301 catttccgga aagttacttc cgaatggcta agtattttca tcggatccca atctttttga
      361 aaatgtgacg aataggattt caagtttaac aacggatgtg tcgatttcat gatgaaggtt
      421 gcctctttat tgattcgatg tattcattta aaacagtcgc tttttcttga tttaatgttg
      481 cacttatcat gccaactttt gaattgctat ctttaaaaga aaagaccgga ataccgggct
      541 atttcggcgt tccgatctta ataaaattat tcttccaata tatgattaat ctacataatt
      601 aattttcgga acctgaatcg cgacgcgaca aaaaggacct tcggagcttt ttgcgatcgg
      661 ttcaaatttc aaataccaat tgtctgcttt gccggggaca tcaaatacca agaaagtatc
      721 taacataccg ccggcttgaa ttgtatcggt aatcaggccg acatcccagc ggtcactcgt
      781 aataaagctg tttgcgatat gtaatttata ctcttgatcg actccatcga taattccatg
      841 taatcgaaac gatctggcag gaaggccttc gagcgttcgg tctgtcaaat tggttatgag
      901 gacacgcgct accaaaaatt ttccttttgc cacctgaccg ctattcgatt ttgacatcag
      961 cggttgataa tagaaagtaa attgaaattt attcccgcaa ttaatttttt gccccaactg
     1021 cgcgggtaag atttccattg tctccggagt ttcggtactt tcaagaattt cgggaacttt
     1081 cggaatctca gttgcttcgg ggatttgtac cggctcaaga gtctccgggg tttcggtaaa
     1141 atcagggata gcggttgatt taatgagagg cgggggaaga acatttttgg tcggagcact
     1201 tttcggagta ctctttcgat aaggttccct gaaattttgt gaggcgggag aaaaagctgc
     1261 caagacggca gcgataagaa tcagtacgat taattttctt ttcatagcga tttatccctt
     1321 acgaaactta tcccgataat atttaattgg ttattttctg cgaacccaaa ttttgtcgaa
     1381 gatgcaggtt gtctgttctc cgccgatccc tacgatcggt cccgtcatcc aagataaatt
     1441 ttctttaata ttatcttgat aggctatcaa aaatactcca tcgatgaaaa aataaccgat
     1501 accgccatat cgaatgatgt caatattgta gatttttccg acttcatggg taccactcaa
     1561 atcataaagc gttgttaaaa ctcgacttcc tgattttgtc tggtaggtat cgattcttga
     1621 tccgagaatt atctctacag tttggcgaga attttctccg acgacagctt cgtttgtata
     1681 ctcaataaaa caactgccgg ttgaatccgg aaattcattc aaaatactta cttctacatg
     1741 aagatggaat tccgataacg tcccgtaata agtttcgctg taaaaagcac ttaatccttc
     1801 aatatcttta atgagtccat caacataatt agtggctttt ctgactccat ttttctgctg
     1861 aataacactg gtgattttta ttccatttga ttcatcttca taaacgtcca gcggtaaatt
     1921 ttcggagaaa tcttcgttaa tcagtagccg gggaaatact ttctccgagt tttttaataa
     1981 attatctccg gtttgtcggg attgatcatc cccttcggat tgtgccgaag caccgaatat
     2041 cataaacagt aaaagaatta cggacagaat ccaaaaatgt cttttcataa ttcattccta
     2101 ttccggatac aaattttgta tctctaaaaa ctcagcctat ttaattcaat aaatacaaga
     2161 ttatgcagat ttttct
//
LOCUS       DAOSMM010000147         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_51237_length_2150_cov_13.564200, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000147 DAOSMM010000000
VERSION     DAOSMM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51237_length_2150_cov_13.564200"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..573
                     /locus_tag="PK898_10500"
     CDS             349..573
                     /locus_tag="PK898_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20496.1"
                     /translation="MNKEKAEHKEKNEPVTPESVEGYPSQCETTGTHTAEGDVGDAEA
                     LRPNCNPQNKLPDDAIPNITPDEVKKKSAK"
     gene            1040..1225
                     /locus_tag="PK898_10505"
     CDS             1040..1225
                     /locus_tag="PK898_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20497.1"
                     /translation="MDMPKMNKTDSELKETKQNFSEKESEKKEFNYSEKDMVNKNQEP
                     EKKPQNPPVKRDNTFLK"
     gene            complement(1456..>2150)
                     /locus_tag="PK898_10510"
     CDS             complement(1456..>2150)
                     /locus_tag="PK898_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20498.1"
                     /translation="TDFRENEDEASLRFSYTAPDGENGFPGNRKVSVEYILNRLNQLI
                     ISYTAETDKPTYLNITNHCYWNLSGDFNRDAYDHILKINADAVYYNDIQFLPTEYVDV
                     KNSPFDFQTPRKIRDAFLNFPDHEQLKLNKGFNHAYLTKGDEAVFLSDNKSGRTLTIS
                     TDYPCVVFYSGGFLDQISGNENQRRPAVGSGLAFEAQYLPDAPQLMGDSLPILFPGMI
                     YRKKIVFGFGTK"
BASE COUNT          739 a          409 c          415 g          587 t
ORIGIN      
        1 tatgattccg tcttcgattt cctgttgcaa gcgagttttc tttcctcttg tcatattatt
       61 tcctgtctgt tgcaatttag tctttagact tcatacctct tatgacttat tatacaaacg
      121 atttccatgg ttttttctta atatgacttt attcatatat aagcagacaa atgtcatata
      181 cagtttgttt tattttcgtc agatattctc ttttcccatc aaaaaagttc attatgtacg
      241 gaaaaataaa tcgtaaaacc gctgataaaa ttaattcagg aattggattc atgatgtccg
      301 atcatttttg attaaacagt ccgataaacg gaggtaggag aagaaaaaat gaacaaagaa
      361 aaagcagaac ataaagaaaa gaatgaaccg gtgacaccgg aatcggtaga aggatatccg
      421 agccaatgcg aaacaacggg aacacatacc gcggaaggtg atgtcggtga tgctgaagca
      481 ttaagaccga attgcaatcc ccaaaataaa cttcccgatg acgctatccc caacataaca
      541 cccgacgaag ttaaaaagaa gtcggcgaaa taaagcaaac gatattgctt tacgagaacg
      601 gattttttaa aaaatccgtt ctcgttcttg aatcatcaat cgtttttctc taacaacttc
      661 aaccgcaaag atattttcat aaaaacaaaa agatgcctga tttgtctctt tgagaattaa
      721 atattccaaa ctgtgcacga aataaaaaaa tgaacccatt acataatcgg atgaaatctt
      781 tcggaaaacg ggaaaatatg aaataaaaat cgccatccga aatgtaatta ttttttatga
      841 acttgtttgc acaagcttta gcaaagtgat acactaaaat ttatttccaa acagacacta
      901 ccggtaaaag attgcgaaag aagccatcaa ttccggcaac ttagatggat agttgattga
      961 agtttcttag atgaaatttc aatccgtttt tttcctcatc ggagggagga aagatttatt
     1021 aaatggaacg gaggagaata tggatatgcc aaaaatgaac aagacggatt cggagttgaa
     1081 ggagacaaaa caaaactttt ctgaaaaaga atcggaaaaa aaagaattca attattccga
     1141 aaaggacatg gtaaataaga accaggaacc ggaaaagaaa ccgcaaaatc cacccgtcaa
     1201 aagggataat acattcctga aataagaatc gccgagaaga tttctctgac tacaattcgg
     1261 gagaaggacc ggatgcttcc gtacggcaag catggatgat atttgtatgc gggaatattt
     1321 tcgaatttga gataaaggga ccggctgaaa agaacggaac cggccggatc ccctaagaac
     1381 acaaacataa gaaaaaccgt aaaccccatc cggttcggcg cttccgatta agccgagatg
     1441 gggtttaaat ttatctcatt tcgttccgaa accgaaaaca atctttttcc gataaatcat
     1501 ccccggaaat aaaatgggca gagagtcacc catcaattgg ggagcgtcgg gtagatattg
     1561 cgcttcaaat gccaatccgg agccaaccgc cggtctccgc tgattttcat taccggagat
     1621 ctgatccaga aatccccctg aataaaaaac gacacaagga taatcggtgg atattgtcaa
     1681 tgttcttccg ctcttgttat ccgataaaaa gacagcctca tcgccttttg tcaagtaagc
     1741 atgattaaaa cctttattca gtttaagttg ttcatgatcg ggaaaattca aaaaagcatc
     1801 gcggattttt cgcggagttt ggaaatcaaa aggggaattt ttaacatcga catattcggt
     1861 gggtaaaaat tgaatgtcat tgtaataaac cgcatcggca tttattttaa gaatatgatc
     1921 gtaggcatcc ctattaaaat cccccgatag attccaataa caatgattgg ttatattgag
     1981 ataagtgggt ttgtccgtct cggcggtgta tgaaataatg agttgattca gcctgttcaa
     2041 gatatattct accgagactt tccgatttcc cgggaatccg ttttcgccat ccggtgccgt
     2101 ataggaaaac cgaagcgaag cctcatcttc attttcccta aaatcggtca
//
LOCUS       DAOSMM010000148         2078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_53648_length_2078_cov_5.891745, whole genome shotgun sequence.
ACCESSION   DAOSMM010000148 DAOSMM010000000
VERSION     DAOSMM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53648_length_2078_cov_5.891745"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..>437)
                     /locus_tag="PK898_10515"
     CDS             complement(256..>437)
                     /locus_tag="PK898_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20499.1"
                     /translation="GGRVGDEGLLFLIFNSFLIIFLTGIPIRNNLSYSDRKNRILKTD
                     DFHDYLFASIFLDRQ"
     assembly_gap    438..537
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <538..1948
                     /locus_tag="PK898_10520"
     CDS             <538..1948
                     /locus_tag="PK898_10520"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HOU20500.1"
                     /translation="GGRNVSIPSPFGRGQGEGNTENPTDQQPKSMNFSLFPDRPSDIA
                     EYQEKQPLIPNPSPVGGRNVSIPSPFGRGQGEGSVQDGNNMINEINQSPYRVRLSEET
                     LRRARELRKNCTDAEQFLWQILRDRQLNGLKFRRQHPFNGYFLDFYCLEKKLAIEIDG
                     SGHAEDQQQNYDQVRTDALKKEGITVLRFWNNEVFENPEGVLETILDTAFSIKGSNMH
                     EDDRSPLQDLFLRQWKENAAESNGGNYWNPPEWVTIETDGCPLPPFKWNESRRAVLRA
                     ELDAYYAALYGLNRKQLRYILDPADLTAGELKNILDPSEEVTDPLDPEGYAERTAASD
                     FPGETFRVLKKNEIQEFGEYRTRRLVLEAWERLSANGELPEPAYERNESSPSRQEPLA
                     PTDKAAVPTPARKSANPTADFAARAKDKSGSPNQIPLSDFGLYKCTVCEKMVMGFEKE
                     NHEREKHGGKKVEWKKMSQ"
BASE COUNT          594 a          463 c          442 g          479 t
ORIGIN      
        1 atataattac tcataatcta tcgtaccgga ttcttgccgt cacagcaggg tgcggagaag
       61 attaagtttt ttgaagaatt caacgtgaat tcgaataaga aaatcaaccg aaatcagccg
      121 tatcgattat gatttgttaa caatttaaca ggtgaaatca tgaatgtttt ctcattttcg
      181 gaaaatactt tcctatatca tactaattat tgattatggt attgtaaaac gacagcggca
      241 aagagattga aacagtcatt gtctatccaa aaatatactt gcaaataggt aatcgtgaaa
      301 atcatcggtt ttcaaaattc gatttttccg atcagaatag ctcaagttat ttctaatcgg
      361 tataccggtc agaaaaatga tcaaaaaact gttgaatatc aggaaaagca acccctcatc
      421 cccaaccctt cccccgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
      541 gggaaggaac gtctctatcc cctcgccctt cgggagaggg cagggtgagg gcaatacgga
      601 gaaccctaca gatcaacaac caaagagtat gaacttttcc ctcttccccg atcggccatc
      661 cgatattgcc gaataccagg aaaagcaacc cctcatcccc aacccttccc ccgtcggggg
      721 aaggaacgtc tctatcccct cgcccttcgg gagagggcag ggtgagggct ctgttcaaga
      781 tggaaataat atgatcaatg agatcaatca gtccccttac cgagttcgcc tttcggaaga
      841 aacgcttcgc cgagcccgag aattgcgtaa gaattgcacc gatgccgaac agtttctttg
      901 gcagattctg cgagaccgcc aactcaacgg cctaaaattc cgccggcaac atccctttaa
      961 tggctatttc ctggattttt actgtctcga aaaaaaattg gcgatcgaaa tcgacggaag
     1021 cgggcatgcc gaagaccagc agcagaatta tgatcaggtc cgaacagacg ctctgaaaaa
     1081 agaaggaatt accgtgcttc gattttggaa taatgaagta tttgagaatc cggaaggagt
     1141 cttagaaacg atcttagata ccgccttttc catcaagggc tcaaatatgc atgaagatga
     1201 tcgatctcca ctgcaagatt tatttcttcg ccaatggaaa gaaaacgcag cggaaagcaa
     1261 cggaggaaac tattggaatc cgccggaatg ggtaacaatc gagactgacg gttgtccttt
     1321 gccaccgttc aaatggaatg agtctcgcag agccgtgtta cgcgccgaat tggacgcata
     1381 ttatgcagcg ctttacggac tcaatcgaaa acaattgcga tatatccttg atccggctga
     1441 tctgactgca ggagagctga aaaatattct tgatccatcg gaagaggtaa ccgatcccct
     1501 cgatcccgaa ggatatgccg aacgcaccgc cgccagtgac tttcccggag aaaccttccg
     1561 tgtactaaag aaaaacgaga tccaggaatt cggcgagtat cgtacccgtc gtctggtatt
     1621 ggaagcatgg gaaaggttat ctgccaacgg tgaattgcct gaaccggcat atgagcgtaa
     1681 tgaatcctct ccttcacggc aggaacctct tgcgccaacc gataaagccg ctgttccgac
     1741 accggctcgg aaatctgcca atcctaccgc tgactttgct gcccgggcta aagataaatc
     1801 aggatccccc aaccagatcc ctttatccga tttcggttta tacaaatgta cggtttgcga
     1861 aaaaatggtc atgggatttg agaaagaaaa tcatgagcgg gaaaaacatg gagggaaaaa
     1921 ggtggaatgg aagaaaatga gtcaataatt ctccccgccg gcacctattc ttttttcgct
     1981 cgcagaaatg aatcctcttt tcttccgaca gaatggaagc cttcgaaaaa tgacgtttcg
     2041 gtctcataaa ctatgatctg cgcttgtgaa tgaagagc
//
LOCUS       DAOSMM010000149         2017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_55912_length_2017_cov_8.854230, whole genome shotgun sequence.
ACCESSION   DAOSMM010000149 DAOSMM010000000
VERSION     DAOSMM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55912_length_2017_cov_8.854230"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="PK898_10525"
     CDS             <1..1472
                     /locus_tag="PK898_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOU20501.1"
                     /translation="KSMAINFGTDGWRAVISDTFTFANLRVVSQAVADAVKSTAWEDL
                     SKGAEIANKDRIVVGYDTRFLSDRYAKEVARVLAANGFKVFLSQSDAATPVISFATKQ
                     LGAIAGVMITASHNAPRYNGVKLKAAYGGGALPEQCKLVEVYLMDNETLGRGPNLLEY
                     DKARELGLITTFNPLLEYSHHIRKLIDFDTIAEAKDLRIVVDSMYGSGRGVIRNLLGG
                     TGVEVNEIRAELNPGFGGVHPEPIYRNLGALASALAAGLGNLGLATDGDADRIGAMDE
                     RGNFIDPHKIMALSIKYLHEKRGLSGALVRTVSTTRMIDCLGKKYGLEVFETPVGFNH
                     ISDHMMERDVLIGGEESGGISFKGHIPEGDGIIMGLLITEMVAAYHATLGELVRQLED
                     EIGAFYYDRTDLRLKHPVAKDEMIKSLVNNAPASIGGETIQQISTLDGVKYIMADDSW
                     LLIRPSGTEPVLRVYAEGRSPQMVKELLGFGNLVAETVK"
     gene            complement(1559..>2017)
                     /locus_tag="PK898_10530"
     CDS             complement(1559..>2017)
                     /locus_tag="PK898_10530"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOU20502.1"
                     /translation="TMGVYIIDPGTAKSGKTYWVTVMLGIAVAVNLICNAILTPLLGL
                     YGAVLSELIGYASAMIGRWIISNRFFPIQWNYRYFSRAVIFYSLFASAQTFIIFSDLT
                     WLWGFLIRSGLGIIFIIILWNSMDSVSKKTILNILNNFLKKINITKKPVQ"
BASE COUNT          559 a          407 c          524 g          527 t
ORIGIN      
        1 cgaaatctat ggccattaat tttggaacag acggatggcg ggcggttatt tccgacactt
       61 ttacttttgc gaatttgcgc gttgtttcac aggcggttgc ggatgcggtg aaatcgaccg
      121 cttgggaaga tttgtcaaaa ggagcggaga ttgccaataa agatcgaatt gttgtcggtt
      181 atgacacccg atttttatcg gatcgttacg caaaagaggt ggcacgggtt ctggctgcca
      241 acgggtttaa agtcttttta agccaatctg atgccgcgac gccggttatt tcgtttgcaa
      301 caaaacaatt gggagcgatt gccggcgtaa tgatcaccgc ttcgcataat gctccgcgct
      361 ataacggtgt taaattgaaa gccgcttacg ggggaggtgc tcttcccgaa caatgcaaat
      421 tagtggaagt ctatttgatg gacaacgaaa cattgggtcg cggaccgaac ttgttggagt
      481 atgacaaagc ccgtgaactg ggtttgatta ccacgttcaa tcctctcctc gaatacagcc
      541 atcatattcg aaaattaatt gattttgaca caatcgctga agcaaaagat ctacggattg
      601 ttgtggattc catgtatggt tccggccggg gggtgattcg taatctgtta ggcgggaccg
      661 gcgtggaagt caatgaaatc cgagcagaat tgaatccggg ttttggaggg gtccatccgg
      721 agccaattta tcgtaatttg ggcgcattag ccagcgcctt agcggccggg ttggggaatt
      781 tggggctggc gacggatggg gatgcggatc gaatcggtgc gatggatgaa cgggggaatt
      841 ttatcgatcc tcataaaatt atggcgcttt ctattaaata tctccacgaa aagcgcgggt
      901 tgagcggagc attggtgcgg acggtttcca ccacccgaat gatcgattgc ttaggcaaga
      961 aatatggatt ggaagttttt gagacaccgg tcggtttcaa tcatatttcc gatcatatga
     1021 tggagcggga tgtgttgatc ggcggggaag aatccggtgg aatttcgttc aagggtcata
     1081 tcccggaggg tgacggtatc ataatggggc tgctaatcac agaaatggtt gcagcttatc
     1141 acgcaacatt gggcgaattg gtccggcaac ttgaggatga aatcggagca ttttactatg
     1201 accgaaccga tctgcgcctc aagcaccctg tggcaaagga tgaaatgatt aagtcgttgg
     1261 taaataacgc tcccgcttca atcggcggcg agacgattca acaaatcagc accctggatg
     1321 gcgtcaagta catcatggcg gatgattctt ggttattaat tcgcccttcc gggacggaac
     1381 cagtcttgcg agtatacgcc gaaggccgtt caccgcagat ggtgaaagaa ttgctcggct
     1441 ttgggaattt ggttgccgaa acggtaaaat aacgattata gataaattta acccgacgag
     1501 ttcgatttat tatggaaaaa atcggactcg ttttaattgt attgcttgaa aagacaggtt
     1561 attggacagg cttctttgta atattaatct ttttaagaaa attattcagg atatttaata
     1621 tagttttctt tgaaaccgaa tccatgctgt tccagaggat aatgataaag ataattccta
     1681 accccgatcg aatcagaaaa ccccataacc atgtcagatc cgaaaaaatg ataaaagttt
     1741 gcgccgaagc aaataagctg tagaatataa cagcccggct gaaatatcgg taattccact
     1801 gaatagggaa aaatcgattt gaaataatcc aacgtccgat catcgcagag gcgtatccga
     1861 tcaattccga aagcactgcg ccgtataaac ccagcaaagg ggtcaggatc gcattacaaa
     1921 tgagattaac tgcaaccgca atacccaaca tgaccgttac ccaataggtt ttgccggatt
     1981 tggcggtgcc cggatcgatg atataaactc ccatcgt
//
LOCUS       DAOSMM010000150         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_57462_length_1976_cov_15.664758, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000150 DAOSMM010000000
VERSION     DAOSMM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57462_length_1976_cov_15.664758"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1974
                     /locus_tag="PK898_10535"
     CDS             133..1974
                     /locus_tag="PK898_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOU20503.1"
                     /translation="MSYYVHQPKKLADVTIDLVNVAMGKAPADLIIRNGKLVNVNLGI
                     VQNNMDVVVKHGFIAYVGRDTENKIMADENTHIVDAKGRYLVPGLIDSHVHIESSMMD
                     PRHFVEGILPHGTTTICPDNHEIANVLGLKAVELFHNSAKGLPIKFLLAMPVCVPSVI
                     GFENAGAEINADDVTTAYSKGWAQLQGEQMNFPGVIHGDEHVHSIIKASLDAGVILTG
                     HYSSNEIDQGLNAFAATGISCCHEATTADAVMKRSELGFYSQLRYGSAWLDLPNTIKA
                     YTENPGLDTRYLTICTDDVNAATIIREGQMDRAVRMAIKNGVPPVIAVQMATLNAAQL
                     LEKARWIGSISPSRAADILIVSDLAEFVIDEVYSDGVLVAKNGILTVELEPYHYPEYS
                     LKTVHLNLLKPEDFRIPANTDEPVNIRAIEIQSGMVNTIEKTKKMHPINGMLESDVSR
                     DLAKAFIFYRHAPKEGVSNSRGCGFVTGTKFNANCAYASTVSHDCHNLLVVGTGDSAM
                     ALAANEVIKSDGGIAIVVDGDLAAKIELPLAGLMSLEPIDIVARKIEAVENALKKAGA
                     LYESIEMTLSLLGLIVIEELHLSNKGLVELRSNSPLNFVDLIKNKEE"
BASE COUNT          569 a          407 c          488 g          512 t
ORIGIN      
        1 gaaatgatcc cttatttatt taccttgatt gtgatgatta tcggcgcaaa cgaagcccgc
       61 agaaaaagga tcggcgcacc ggctgcattg ggtcaacctt attcccgtgg gcagagagga
      121 aaatagactt ttatgtcata ttatgttcat caaccgaaaa aacttgcgga cgttacgatt
      181 gatttggtca atgtcgcaat ggggaaagct ccggccgatt taattatccg gaacggaaaa
      241 ctggttaatg tgaatctcgg catcgtccaa aataatatgg atgttgttgt aaaacacgga
      301 ttcatcgctt atgtcggccg ggataccgaa aataaaatta tggcggatga aaatacccac
      361 attgtggatg ccaaaggccg ttatttggtt cccggtttga tcgacagcca tgttcatatt
      421 gaaagttcaa tgatggatcc ccgccatttt gtagagggaa tattgcctca cggtaccaca
      481 actatttgtc cggataatca tgagattgca aatgtattgg gattgaaagc tgtggagctg
      541 ttccacaatt cggcgaaggg attaccgatt aaattcctgc tggcaatgcc ggtttgtgtc
      601 ccgtctgtga tcggatttga aaatgccgga gcagagataa acgccgatga tgtcacaacg
      661 gcctattcca aaggttgggc acaattgcaa ggcgaacaga tgaattttcc gggtgtaatt
      721 catggcgatg aacacgtgca ttcaattatc aaagcttctc ttgatgccgg tgtcatcctg
      781 accggtcact actccagtaa tgaaatcgat cagggactaa atgcttttgc cgcaaccgga
      841 atctcctgtt gccatgaagc aacaacggct gatgcggtaa tgaaacgctc cgaactggga
      901 ttttacagcc agctgcgtta cggatccgct tggttggatt tgccgaatac cataaaagct
      961 tacacggaaa atccgggctt agatacccgc tacctgacaa tctgtacgga tgatgtaaat
     1021 gccgcaacga ttatccggga aggacaaatg gatcgggctg ttcggatggc aatcaagaac
     1081 ggtgtccctc cggtgatcgc tgttcaaatg gccacattaa atgccgctca attgctggaa
     1141 aaagcccgat ggatcggttc gatttcaccg agtcgggcgg cggatattct tatcgtcagc
     1201 gatttggcgg aattcgtaat cgacgaagta tatagtgacg gagttttggt ggcaaaaaat
     1261 gggatcttaa ccgtcgaact cgaaccgtat cattacccgg aatattcctt aaaaacagtc
     1321 cacttgaatt tgcttaaacc ggaagacttc cgaattccgg caaatacaga tgaaccggtc
     1381 aacattcgag caatcgaaat tcaatcggga atggtgaaca caattgaaaa aacaaaaaag
     1441 atgcatccga ttaatggaat gcttgaatcg gatgtgtcac gggatttagc caaagctttt
     1501 atcttttacc gccatgctcc gaaagaggga gtgagtaaca gccgcggatg tggatttgtc
     1561 acagggacga aattcaatgc aaattgtgca tatgcgtcta ccgtctctca cgattgtcat
     1621 aaccttctgg tagtcggaac cggtgactcc gcaatggctt tggctgccaa tgaagtcatt
     1681 aaatccgacg gcggaatcgc aatcgtagtg gatggggatt tggctgccaa aattgaattg
     1741 ccgctcgcag gtttaatgtc tttggaaccg atcgatatcg tcgctaggaa aatcgaggct
     1801 gtagaaaatg ctctgaaaaa agccggggct ttgtatgaat cgattgaaat gacattaagc
     1861 ctgttgggac tcatcgtgat cgaagaactt catttgagca ataaggggct ggttgaatta
     1921 cgatcaaatt caccgctgaa cttcgttgat ttaatcaaga ataaagagga ataaaa
//
LOCUS       DAOSMM010000151         1962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_58091_length_1962_cov_12.649712, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000151 DAOSMM010000000
VERSION     DAOSMM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58091_length_1962_cov_12.649712"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PK898_10540"
     CDS             <1..725
                     /locus_tag="PK898_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HOU20504.1"
                     /translation="VKSKFEEILRERANAFTANLAVMVNNNAALSKCDPMSVISAAVV
                     AASLDLPIDPNLGFAHIVPYGDKAQFQIGVKGFIQLAMRSGQYSRIGVTEIYEGQLLD
                     ENPLTGEYTFDFKAKKSETIIGWAAYFKTVNGFEKTLYWPVEKIKKHGLRFSQTFKKG
                     FGLWKDDFDSMAAKTVLKALLSKWGILSTEMQNAVKFDQGVVKSVETQEVEYLDNDPI
                     AAAFDDGPVKGKKVTTEKLELK"
     gene            722..1186
                     /locus_tag="PK898_10545"
     CDS             722..1186
                     /locus_tag="PK898_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20505.1"
                     /translation="MSAPFCNILKKNLKKRDKYSGISNAIIYRVEHELHENITTDLKN
                     FLIDYDNNVFCFEKCSVELNANSLSVSIAMLFTAQSHVPKKIRESNYRKIKESRDHFL
                     FQYETTKYFKVLWFMIRDVDDRIFKMVNKSFIEKYFDKMISDGRELFIINQY"
     gene            1188..1823
                     /locus_tag="PK898_10550"
     CDS             1188..1823
                     /locus_tag="PK898_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HOU20506.1"
                     /translation="MINQRSPEWFEIRRGKFTASEIHKLMGQKGMGKTGETYVMEKVV
                     EELGATIPPVSTYAMERGTELEPYAKAHYSKAFNCSVSPAEFIIAPWCDEAGASPDGI
                     VTDWNQPAESKLIEIKCPLNPVNHLQNFMVMSADDLKSLRPEYYWQIQMQMAVCGFEV
                     CDFVSYYPEIDEDFRMIAITIKLNEADIDLMKTRISEAVEMKREMLKKIRL"
BASE COUNT          630 a          386 c          461 g          485 t
ORIGIN      
        1 acgtcaaatc aaaatttgag gaaatcctcc gcgaacgggc aaacgccttc accgcaaacc
       61 ttgcggtaat ggtgaacaac aatgccgccc tgtcaaaatg cgacccgatg tccgtcatat
      121 ccgccgccgt ggtggccgct tccctcgatc ttcccattga ccctaacctg ggatttgctc
      181 acatcgtacc atacggcgac aaggcccaat ttcagatcgg cgttaaggga ttcattcagt
      241 tggcaatgcg atccgggcag tattcccgca taggggtcac cgaaatctat gaggggcagt
      301 tgttggacga aaacccatta acaggggaat acacctttga tttcaaagcg aaaaaatcgg
      361 aaacgatcat cgggtgggcc gcctatttca agaccgtgaa cgggtttgaa aaaacgcttt
      421 attggccggt agaaaaaatc aagaagcacg gactccgctt ttctcagact ttcaaaaaag
      481 ggttcgggtt atggaaggat gattttgact ctatggccgc taaaacggtt ttaaaggccc
      541 ttttgtcaaa gtggggtatt ctatcaaccg agatgcaaaa tgccgttaaa ttcgatcagg
      601 gggtcgttaa atcggtagaa acgcaagagg tggaatattt ggataacgac cctatcgccg
      661 ccgcattcga tgatgggccg gtgaagggga agaaggtaac aacggaaaaa ctggaactga
      721 aatgagcgca ccattttgta atatattgaa aaaaaacctt aaaaaaaggg ataaatattc
      781 tggaatatca aacgctatta tttacagggt tgaacatgaa ttgcatgaaa acattactac
      841 cgatcttaaa aattttctaa tagattatga taacaatgtt ttctgttttg aaaaatgttc
      901 tgttgaatta aatgctaatt cattatctgt tagtatagca atgttattca cggcacaatc
      961 tcatgttcct aaaaagataa gagagagtaa ttaccgtaag ataaaagaat cacgagatca
     1021 ttttttattt cagtatgaaa caacaaaata ctttaaagtt ctatggttta tgattaggga
     1081 tgtagatgat agaatattta aaatggtaaa taaatcattc attgaaaaat attttgacaa
     1141 gatgatctca gatggtagag aattgttcat aataaatcaa tattaaaatg attaaccagc
     1201 gatcccccga atggtttgaa atccggagag gaaaatttac ggcatcagag atacacaagc
     1261 tgatgggtca gaagggcatg ggcaaaacgg gtgaaaccta tgtcatggaa aaggtggtcg
     1321 aagaactggg ggcgacaatt cccccggttt ccacctacgc aatggaacgg ggtacggaat
     1381 tagaaccata cgcaaaggcg cattattcaa aggcgttcaa ctgttcggta tcaccggcgg
     1441 agtttatcat cgccccgtgg tgcgacgagg cgggcgcatc acctgacgga atcgtaacgg
     1501 attggaacca accggcggag tcaaaactca tcgaaatcaa atgcccgtta aatccggtga
     1561 accaccttca gaattttatg gtaatgtcgg ccgatgacct gaaatctttg cgccctgaat
     1621 attattggca aattcagatg cagatggcag tgtgcggatt tgaggtctgc gatttcgtga
     1681 gttactaccc tgagattgac gaggatttca ggatgatagc gatcaccata aaattaaacg
     1741 aggctgacat tgacctgatg aaaacccgca tttcggaggc ggtagagatg aaacgtgaaa
     1801 tgctaaagaa aatcaggcta tgagttgcaa aatcctcaaa atcacaatcg tcaagaccct
     1861 gccgtttaca ggaggcccga ttattaatgt catatatccc gaaggcgtta ccgatgacat
     1921 tgaggcatac cggaaggaaa tgaaggccga acacggtgcc ga
//
LOCUS       DAOSMM010000152         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_59365_length_1932_cov_13.363346, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000152 DAOSMM010000000
VERSION     DAOSMM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59365_length_1932_cov_13.363346"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1529)
                     /locus_tag="PK898_10555"
     CDS             complement(90..1529)
                     /locus_tag="PK898_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU20507.1"
                     /translation="MKNLKLQFILSHTLPVLITIPLVGVALSYAMETHLILKDMKQTI
                     QDQAALITQYSEGNPDVLKEEQAAEQMIQEIRPNLVTGVILFDRAWNVLATTDDDKNE
                     ITQRFPPPMMEMQFGQSGLEPVINISKYNPFKHSEDIIEMMVPVTNSDNQLLGMIRLN
                     FPVTYFEEQLKQTSDRIILILICGVILGVLFGLINAISLEKRLSRTTGAIYDLSLGIR
                     NNPLPEEGPEEMQKLAKAFNSLSQKLDSSEQTRTKLVSYLTHELGRPLGGLSSAVDAL
                     QMGAAQDEKLVQDLTIGMKNEIKRLELLVGDIALLREKTDPINLYVMKPVPLSAWLNE
                     VYSYWAEYARDKDIKLVDEIDQNLPTIKMDENRMYQAVGNLLSNAIKYSPNGKTVTFR
                     AVQTDDEIQIQVTDQGCGIDPEDLPHIFESFYRGSENKRIIQGMGLGLTISQDVVQAH
                     RGTITVESEKGAGSTFTIHLPLKNISPEK"
     gene            complement(1526..>1932)
                     /locus_tag="PK898_10560"
     CDS             complement(1526..>1932)
                     /locus_tag="PK898_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU20508.1"
                     /translation="YITKPFDFDVLLAHVKAVLRRRENPVQEPEENILQVGDLKLDFP
                     QHKAEIGGKELELSPKEFELLTALALNADHIIGTDNLLKRVWGAEYTGDPQVLYVHIR
                     WLRSKIEEDPDHPKRILTVRSVGYKFNSTLSE"
BASE COUNT          484 a          445 c          443 g          560 t
ORIGIN      
        1 tcgggataag cttgcaactg aagattttat aatgatcgtc agccatggaa acgagctgtt
       61 gtcggattgg aagacctgtc cggttcatac tatttttcgg gcgaaatgtt cttgagcgga
      121 aggtgaatgg tgaaagtgct gccggcgcct ttctcgcttt cgacggtaat cgtccctcga
      181 tgagcttgga cgacgtcttg agagatagtc aaacccagcc ccattccttg gatgatgcgt
      241 ttattttccg aacctcgata gaaggattca aaaatatgcg ggagatcttc tggatcaatg
      301 ccgcagcctt gatcggtcac ttgaatttga atctcgtcat cggtctgaac tgctcgaaag
      361 gtgacggtct tcccgttggg tgaatattta atggcattgc tcaataaatt cccgacggct
      421 tgatacatgc ggttttcatc cattttgatc gtcggcagat tctgatcgat ctcatcgacc
      481 agtttaatat ctttatcccg cgcatattcg gcccaatagc tgtagacttc gttcaaccaa
      541 gcggacaacg ggaccggctt catgacatat aggttgatgg gatctgtttt ttcccgcaaa
      601 agcgcgatat cacccacgag taattccaaa cgtttaattt cgtttttcat cccgatggtc
      661 aaatcctgaa ccagcttttc atcctgtgcc gcacccatct gaagggcatc cacggctgag
      721 gaaagtccgc ccaatgggcg tcccaattca tgggtcaggt acgaaaccaa ttttgtgcgg
      781 gtctgttcgg aagaatccaa tttttgggaa agcgaattga atgctttcgc caacttttgc
      841 atctcttccg gcccttcctc cggcagcggg ttattccgaa tgccgagaga caaatcataa
      901 atggcaccgg tcgtccggct gagtcgtttt tcaaggctga tcgcattgat caaaccgaaa
      961 agcacaccca aaatgacacc gcagatcaaa atcaaaatga ttcgatccga agtttgtttc
     1021 aattgttctt cgaaataagt caccggaaaa ttcaaacgga tcatcccgag gagctgatta
     1081 tcgctgttgg ttaccggaac catcatttcg ataatatctt cggaatgttt gaaaggattg
     1141 tatttggaaa tattgatcac cggttcgaga ccggattgtc cgaattgcat ctccatcatc
     1201 ggcggcggga atcgttgtgt gatttcgttc ttgtcatcgt ctgtggttgc cagtacattc
     1261 caggcgcgat cgaataaaat gactcccgtg accaagttgg gacgaatttc ctgaatcatc
     1321 tgttcggctg cctgttcttc tttcagaacg tcgggatttc cttcggaata ctgtgtgatt
     1381 aacgctgcct gatcctgaat ggtttgcttc atatctttca aaatcaggtg agtttccata
     1441 gcatatgata aagcgacacc caccaaagga atcgtaataa ggaccggtaa tgtatggctc
     1501 aggataaatt gaagtttaag atttttcatt cagataaagt cgaattaaat ttgtacccca
     1561 cgctgcggac cgtcaatatt cgtttgggat gatccggatc ttcttcaatt ttactgcgca
     1621 gccagcgaat atggacataa agaacttggg ggtcgccggt atattccgcc ccccagactc
     1681 gtttcagtaa attgtccgtg ccgataatat gatcggcatt cagcgccaaa gcggttaata
     1741 actcgaattc tttgggggat aactccagtt cttttccgcc tatttcggct ttatgttggg
     1801 gaaaatccaa ttttaaatct cccacctgca aaatattttc ctccggttcc tgtaccggat
     1861 tctcgcggcg gcgtaagacc gctttcacat gggctaagag aacgtcgaaa tcaaagggtt
     1921 tcgttatata at
//
LOCUS       DAOSMM010000153         1859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_62655_length_1859_cov_18.968404, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000153 DAOSMM010000000
VERSION     DAOSMM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62655_length_1859_cov_18.968404"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..>1859)
                     /locus_tag="PK898_10565"
     CDS             complement(58..>1859)
                     /locus_tag="PK898_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOU20509.1"
                     /translation="DLVDELPYEIRISVETPSGEILTATDSDRIVIDHPELWWPNELG
                     GQPLYRVSAELLYEGKVLDTWQRRIGLRTLTIRREKDQWGESFCHHINGIDIFAKGAD
                     YIPEDNILSRITPERTRALLENAVEAHFNTIRVWGGGFYPPESFYETCDELGLLVWQD
                     FMFACGAFRLDEKSEQNMREEFRDNIIRIRNHPSLALWCGNNEIEEFAAKNQWVKTPK
                     QHSEYISLFEYIIPHMVRQYDPQTFYWPSSSSSGGGFDDPQDPSRGDVHYWDVWHGEK
                     PFSDYRKYYFRYVSEFGFQAYPPLQTLQTVTLPEDLNAFSRIMEKHQRNASANGKIMK
                     YIQQTFLYPKDFESLVYTSQLLQAEAIRYGVEYWRSIRNCCMGTIIWSLNDCWPVISW
                     ASIDYYGRWKALHYAAKRFFAPVLLNALETGENTIYPDINAERIFPVKNSVVFNITND
                     TWEEVNSEIIWSHRSNDSQIRHQGKITVTVPPFTAKQTDEILFPDFSTYDDYISFSLF
                     VEGKEISHSSVLLSAPKHFHFLDPHLTVQAAGEELIVRSEAYAKGVFIDNEKGDLKLE
                     DNWFDMDAGEKRIKVLRGTLSNLNVRSVYNIAY"
BASE COUNT          515 a          426 c          392 g          526 t
ORIGIN      
        1 gtcatttgtg aaacgagttt ttttacatcc atgaaatcat ctccttttct gagtgtatta
       61 atatgcaatg ttgtaaaccg agcgaacgtt taaattactg agcgtccctc tgagaacttt
      121 aattcttttt tctccggcat ccatatcaaa ccagttatcc tcgagtttca gatcgccttt
      181 ttcattatcg ataaataccc cttttgcata agcttccgag cgtacgatga gttcctcgcc
      241 tgcggcttga acggtcaaat ggggatcgag gaaatgaaaa tgcttgggtg cgctgagcag
      301 aacgcttgaa tgtgaaattt ctttcccctc cacgaaaagc gaaaaactga tgtagtcatc
      361 ataagtggaa aaatccggga aaagaatttc atccgtttgt ttggctgtga aaggcgggac
      421 agtgacagtt attttccctt ggtgacgaat ctgtgaatca ttggatcggt gagaccagat
      481 aatttcgctg ttcacttctt cccaagtatc gttggtaata ttaaaaacaa ccgaattctt
      541 taccggaaag atgcgttcgg cattgatatc cggataaatt gtattctcac cggtttccaa
      601 ggcgtttaat aaaaccggag cgaaaaagcg cttagcggca tagtgcagtg ctttccaccg
      661 cccgtaataa tcgattgacg cccaggagat caccggccag caatcgttta aactccaaat
      721 aattgttccc atgcaacagt tgcgaatgct tcgccaatat tccactccgt agcgaatcgc
      781 ctcggcttgt aataactgtg aggtataaac cagcgattcg aagtccttcg gatatagaaa
      841 agtctgttga atatatttca tgatcttgcc gtttgcagag gcattccgtt gatgcttttc
      901 catgattctc gaaaaagcat ttaaatcttc cggcaaagtg acggtttgaa gagtttgcaa
      961 cggcggatag gcttggaacc caaattcgga gacataacga aaataatact ttcgataatc
     1021 ggaaaacggc ttttctccgt gccagacatc ccagtaatgg acatctccgc gggacggatc
     1081 ctgcgggtca tcaaaaccgc cgccggaaga cgatgaggaa ggccagtaaa atgtttgcgg
     1141 atcgtattgc ctgaccatat gcggaatgat atattcaaaa agagagatat attcactgtg
     1201 ctgtttcgga gttttgaccc attggttttt agccgcaaat tcttctattt cattattgcc
     1261 gcaccataaa gccagagacg gatgatttcg gatgcggatg atattatcgc gaaattcttc
     1321 ccgcatattc tgttcggact tttcatccaa acggaaagcg ccgcaggcaa acatgaaatc
     1381 ctgccaaacc aaaagtccca gttcatcgca ggtttcataa aaagattcag gcggataaaa
     1441 ccctcctccc caaacccgaa tggtattgaa atgggcttcc acggcatttt ccaataaagc
     1501 cctggttcgt tccggtgtga ttcgactgag aatattatct tcggggatat aatccgcacc
     1561 ttttgcgaaa atatctatcc cgttaatatg atggcagaaa ctttcacccc actgatcttt
     1621 ttcacgacga atcgtcagag ttcttaaacc gatccgtctt tgccaggtat ccaatacttt
     1681 tccttcatat aataattcgg cactaacccg ataaaggggt tgtcctccca attcattcgg
     1741 ccaccacagt tcgggatgat cgataacaat ccggtcgcta tccgtggctg ttaagatttc
     1801 tccggaagga gtctcgacac ttattcggat ttcataggga agctcatcca ccaaatcca
//
LOCUS       DAOSMM010000154         1834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_63901_length_1834_cov_23.073637, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000154 DAOSMM010000000
VERSION     DAOSMM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63901_length_1834_cov_23.073637"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1834
                     /locus_tag="PK898_10570"
     CDS             <1..>1834
                     /locus_tag="PK898_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU20510.1"
                     /translation="TAEDIPTIAAWHSLAYPDSAYREEMTPSLLDLLSGKNNATEYID
                     VSITCKDGSIRHACQSCARTSDRILATYFDITERKNSERLIRQSEERYRNIIEQMEDG
                     CFETDLTGNFTFVNNAECRLLGYKKEELIGKNNLFYADEKSAKEIHRFFVDICKTGKA
                     VKSHELHFAKKDGNRVIHDVSISLLRDTQGKPAGFRGIARDITERKQMEEALRHSEEK
                     YRSTIETIQDGYFEIDLTGKYTFLNDIICQHLKYSREELIGTDNRAYQTPEEAKKTYL
                     AFRQVYETGIPNKALEMEIIRKDGTTGVSEVSISLIRDSSGVPIGFRGISRDITQRKN
                     MEEFLRQSEERYRSIIENMTDGYFEVDLYGKFTYLNDAQCQNLGYARKELIGMSYKQY
                     ADEKINKELARLFYKIYKTEIPVKSYDLSFIRKNGTRAYNEISVTALKNAKREIIGFR
                     GISRDITDRKQMEEALHRSENKYRSIIESIDDTYYETDISGNLTFFNDMLCRHLLYTR
                     EELIGKNSRMFQDEQNFKKTEQFYREVYKTGNPSILEMECIRKDGTKGIFELSLSLIT
                     DKDGKKTGFRGISRDITKRKQMQSLLTESEEKYRTILEEMNDGYF"
BASE COUNT          614 a          400 c          386 g          434 t
ORIGIN      
        1 cacggctgag gacattccca caattgccgc atggcattct ctggcttatc cggattccgc
       61 atatcgtgag gagatgaccc cttcccttct cgacctgctt tcgggaaaaa acaatgctac
      121 cgaatacatc gatgtaagta tcacctgcaa agacgggtca atacgtcacg cgtgtcaatc
      181 atgcgcgcgc acttccgacc gaattttggc cacttatttt gatattaccg agcgcaaaaa
      241 ctcggagaga ttaattaggc agtccgaaga aagataccga aacataatag aacaaatgga
      301 agacggatgt ttcgaaacgg atcttacggg taattttacc tttgttaaca acgccgaatg
      361 cagattactc ggttataaaa aggaagagtt gataggcaaa aacaatcttt tttatgctga
      421 cgaaaaaagc gccaaagaga tacaccgctt ttttgtggat atttgcaaaa ccggcaaagc
      481 cgtcaaatcg catgaattgc actttgccaa aaaagacggt aacagagtca ttcacgatgt
      541 ttctatatca ttactcagag atacccaagg caaacctgcc ggcttccgcg ggatagcgcg
      601 cgatatcacc gaacgcaaac aaatggaaga agccctgcgc cacagcgaag aaaaataccg
      661 cagtacgatt gagactatcc aggacgggta ttttgaaatt gaccttaccg gcaaatacac
      721 gtttcttaac gatattatct gtcagcacct taaatattcc cgcgaggaat taatcggaac
      781 ggataatcgc gcgtatcaaa cgcctgaaga agcgaaaaaa acttatcttg ccttcagaca
      841 agtatacgaa acgggcatac ccaacaaagc attggaaatg gaaattatcc gaaaagacgg
      901 aactaccggt gtttccgaag tttccatttc cttaatcagg gacagttccg gcgtgccgat
      961 cggcttccgg ggaatttccc gcgatatcac ccagcgtaaa aacatggaag aatttcttcg
     1021 ccagtccgaa gaaagatatc gcagtattat tgaaaacatg acggacggtt attttgaagt
     1081 ggatctttac ggaaagttta catatctcaa tgacgcgcag tgccagaatc tcggatatgc
     1141 ccgtaaagaa ttaatcggaa tgagctacaa acaatatgcg gacgaaaaaa tcaacaaaga
     1201 gctagctcgc ctcttttata aaatttataa aacggaaata ccggtaaaat cctatgacct
     1261 ttctttcatc agaaaaaacg gaaccagggc atacaacgaa atttccgtaa cagcactcaa
     1321 aaacgcaaag agagaaatca tcggcttccg gggaatttcc cgcgatatca ccgaccgcaa
     1381 acaaatggaa gaggccctgc atcgctccga gaataagtac cgctctatca ttgagtcaat
     1441 tgatgacact tattatgaga cagacatttc cggcaatctc acttttttca atgatatgct
     1501 ttgccgacac ctcctctata cgcgcgagga actgattggt aagaatagcc gtatgttcca
     1561 ggatgaacaa aattttaaaa aaacagaaca gttttacagg gaggtatata aaactggcaa
     1621 tccttccatc ctagagatgg aatgcataag aaaagacggc acaaaaggaa tatttgaatt
     1681 gtctttatct cttattacag acaaagacgg taaaaagact ggttttcgag gtatctcgcg
     1741 cgatattacc aagcgtaagc aaatgcaaag tttgcttacg gaatcggaag agaagtaccg
     1801 gacaatactg gaagaaatga atgacggtta tttt
//
LOCUS       DAOSMM010000155         1706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_70701_length_1706_cov_7.873410, whole genome shotgun sequence.
ACCESSION   DAOSMM010000155 DAOSMM010000000
VERSION     DAOSMM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70701_length_1706_cov_7.873410"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK898_10575"
     CDS             <1..439
                     /locus_tag="PK898_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU20511.1"
                     /translation="TGIEGSRISMVFQENRLCRHLSGLCNCMLPLKSTEENRKRVRNL
                     LFEVGLDTTAVEKPVFELSGGMQRRVAIIRALVADSDIIFMDEPLKEMDEHTEELVVQ
                     CIRRESGGKTLILSTHNVEDAERFSANLVYLESSFINNHSEKN"
     gene            complement(529..>1706)
                     /locus_tag="PK898_10580"
     CDS             complement(529..>1706)
                     /locus_tag="PK898_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOU20512.1"
                     /translation="NCGQCVAVCPTGALTTPSQVDLIWDALRDPEMHVVISVAPAVRV
                     AIGEEFDYPPGAIETGKMVAAIRKLGFNEVFDTCYAADVTTWEETAEFVKRLQNKENL
                     PHFTSCCPAWIKACETLYPEKIKNLSTCRSPQAIEGSMLVHHYVEEKKMDREKLFVVS
                     VMPCVAKKFEARRPELSRNGHPDVDAVITTSELAKMIKAAGIVFNELSEESFDIPYGF
                     STGAAVIYGMTGGVATAVVREVRYLLTGERTSDLETEPVEGFPGLMRSSVTLPDGTIV
                     KLGIVSGMANVQKVINAIDSKELEFDIVEVMACPGGCVGGGGQPIPNESAQRAKRRKG
                     IITDDIRKDLRVAHDNVLVGELYNRWLKTPNSEIAHEYLHTHYQKRPRDYEITKTKN"
BASE COUNT          466 a          385 c          366 g          489 t
ORIGIN      
        1 aaccgggatc gagggaagca gaatatcgat ggtttttcag gaaaatcgat tatgcaggca
       61 tctgagcggt ttgtgtaatt gtatgctccc attgaaatcc acagaggaaa accggaaaag
      121 agttcggaat ttattattcg aagtgggatt ggatacaacg gcggttgaga aaccggtttt
      181 tgaattaagc ggtgggatgc aacgccgagt tgcgatcatt cgcgcattgg tcgcagattc
      241 cgacataatt tttatggatg aaccgttgaa agaaatggat gaacatacag aagaacttgt
      301 tgtccagtgc atcaggagag aatccggcgg taaaactctt attctgtcta ctcataatgt
      361 tgaagatgcg gaacgttttt cggctaattt ggtttattta gaaagttcat ttattaataa
      421 tcattcggaa aaaaattgaa attccctttc atggcttgaa taccgattat taaaaagagg
      481 agagattctc cgtaaagatc tctcctcttt atatttctaa agagcaaatc aattctttgt
      541 tttggttatt tcgtagtctc gcggtctttt ttgataatgt gtatgaagat actcatgagc
      601 gatttcactg ttaggagtct tcaaccatct gttatacaat tcaccgacca gtacgttatc
      661 atgagcaacc cgtagatcct tacgaatatc atctgtgatg atcccttttc tgcgttttgc
      721 ccgttgtgca ctttcgtttg gtatcggctg tccgccacct cccacacaac cgcctgggca
      781 agccatgact tccacaatat cgaattccaa ttctttcgag tcgatggcgt tgatgacttt
      841 ttgaacattt gccatcccgc tgacgattcc taatttaaca atcgtaccat caggcaaagt
      901 aaccgatgat cgcatcaacc cggggaatcc ttcgaccggt tccgtctcca aatcacttgt
      961 tctttcacct gttaagagat agcgaacttc ccggaccaca gcggttgcga caccgccggt
     1021 cataccgtaa atgaccgcgg caccggttga aaaaccataa gggatgtcaa aagattcttc
     1081 agacagctca ttgaaaacga ttccggctgc cttaatcatt tttgccagtt ccgaagtagt
     1141 aataacggca tcaacatccg gatgaccatt ccgacttaat tcggggcggc gggcttcaaa
     1201 tttcttcgca acgcaaggca tcactgagac aacgaataac ttttcccggt ccatcttttt
     1261 ttcttccaca taatgatgaa ccaacatgga accttcaatg gcttgcgggc ttctgcatgt
     1321 cgaaagattt ttaatctttt cgggatataa tgtctcgcaa gctttaatcc aagccggaca
     1381 acaagaagta aagtggggta aattctcttt attctgtaac cgtttaacaa actctgcggt
     1441 ttcttcccag gtagtgacgt cagcggcata acaggtatcg aatacttcgt tgaaaccgag
     1501 cttacgaatt gcggcaacca tttttcctgt ttctatggct cccggcggat aatcaaattc
     1561 ttcaccgatt gcaacgcgaa ccgctggagc cacgctgata acgacatgca tttcgggatc
     1621 acgcaacgca tcccaaatta aatccacttg tgagggggtt gtcaaagcgc cggttgggca
     1681 aaccgcgaca cattgaccgc aattta
//
LOCUS       DAOSMM010000156         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_74074_length_1650_cov_14.016301, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000156 DAOSMM010000000
VERSION     DAOSMM010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74074_length_1650_cov_14.016301"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PK898_10585"
     CDS             <1..992
                     /locus_tag="PK898_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20513.1"
                     /translation="LITGPDSFEIQMSAKGTVKGNHISLFGLDGTPLTEIDLSVNPTN
                     PNQLIISGTDMADQSSKTYYLEISNREISLDSDTDSIFSLRFKPRETILIEIPSSESE
                     VELAFDKKAYRINGTVSSPEQTIEIELELNPLKNLIALALNGTPVIWIESDPVKNSLD
                     LVFADAELLMEFDDDAHEILGTFYTTDQQSQFKLICDKPSNSLALDMVIEGEMTELFK
                     LAFSKQANGVSLNSAGGLIFSATIDEEKGTMTINDSSGFRVLDESQILDLINSSIKTG
                     PEAETEVIDNFLNEEEETAVPEEEIIPTAAPISSPTEIPTTVPVAIPTLDAGI"
     gene            1137..>1650
                     /locus_tag="PK898_10590"
     CDS             1137..>1650
                     /locus_tag="PK898_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU20514.1"
                     /translation="MIKIKKPDRIYFRQILKLAVPLILQYLLSSSVTLVDNVMVGALG
                     DVSLAAVNICTQFYMRLFHIVIFGLVSGASILTTQYWGIHDSKNIHRIMGLEIIVGSG
                     LGVLFSILTAFFPEKILGFYSEDPQVIRAGSLYLSTFSLIFLLHPIALIYASAHRAIG
                     DTKLPMIAIAL"
BASE COUNT          461 a          369 c          365 g          455 t
ORIGIN      
        1 ttttgataac cggacccgat agtttcgaaa tccaaatgtc agccaaaggc acggtgaaag
       61 gaaaccacat ttctctcttc ggcttagacg gaacaccctt gaccgagatt gacctgtccg
      121 ttaatccgac caatcccaat caattgataa tctccggaac tgatatggcg gaccaaagca
      181 gtaaaaccta ttatctggag atcagtaatc gagagatatc cttggattcg gatacggatt
      241 ccattttttc actgcgtttc aagccgagag aaacgattct gatagagatt ccttcctccg
      301 aaagcgaagt ggaactggct tttgataaaa aagcatacag aatcaacggc acggtcagct
      361 caccggaaca aaccattgaa attgaattgg aattgaaccc gctgaaaaat ctgatcgctt
      421 tggcgcttaa cggcactccg gtcatttgga tcgaatccga tccggtcaaa aacagtttgg
      481 atcttgtatt cgccgatgcc gaactcctga tggaatttga tgacgatgca catgaaattc
      541 tcggcacatt ctatacaacc gatcagcaat cgcagttcaa actgatctgt gataaaccga
      601 gtaattcgct ggcgttggat atggtgattg aaggagagat gacggaactc ttcaagcttg
      661 ccttcagtaa acaggcaaac ggtgtctcac tgaattcggc cggaggattg attttttccg
      721 ctacaatcga cgaagaaaaa ggtaccatga cgatcaatga ttcttcgggc ttcagagtat
      781 tggatgaatc tcagatcctt gatttaatca attcttccat aaaaacagga ccggaggcgg
      841 aaacggaagt catcgataat ttcttaaatg aagaggaaga gaccgctgtt ccggaggaag
      901 aaataattcc gacggcagct ccgatctcat cccctacgga aataccgaca acggttcccg
      961 tagcgattcc cacgctggat gcgggaatat aaatcggggg gaagatcgat taaaatagtt
     1021 tgaaataact tacaatttta taaggaactg agctgcgccg gcaaaggtca tatttatcag
     1081 ctcagtttct ttttgcaaaa tgctcggaag tttcaatccg cgcagtcagt gaggtcatga
     1141 taaaaatcaa aaaaccggac agaatttatt ttcggcagat cctgaaactg gcggtaccgt
     1201 tgattctcca atacctgtta tcttcttccg tgaccctggt ggacaacgtc atggtcggag
     1261 cattgggaga tgtttctctg gcagccgtaa acatctgtac gcagttttat atgcgtttat
     1321 tccatatcgt gattttcggt ttggtctccg gtgcttcaat tctgacgact caatattggg
     1381 gcattcacga ctcaaagaac attcatcgta ttatggggct tgaaatcatc gttggttccg
     1441 gactcggcgt tttgttttcg atcttaaccg cctttttccc ggagaaaatc ctgggattct
     1501 attccgaaga tcctcaagtc atccgagccg gatcgttata cctgtccact ttttcgctga
     1561 tttttttgct tcaccccatc gcattaatct atgcaagtgc tcatcgggct atcggtgata
     1621 caaaattacc gatgatcgct atcgctttat
//
LOCUS       DAOSMM010000157         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_78656_length_1580_cov_13.846557, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000157 DAOSMM010000000
VERSION     DAOSMM010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78656_length_1580_cov_13.846557"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..917
                     /locus_tag="PK898_10595"
     CDS             48..917
                     /locus_tag="PK898_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU20515.1"
                     /translation="MVETQFDKKMKRERIISKINTGLVLIFLILVTLIVLYPLVYLVS
                     AAFSPGNSISSLNIIPFGDGFTIEHYRRLFRETNYLNWFKNTLIIAAGTSISTVAVCA
                     IAAYVFSRFRFPFKKGMMLLLLILQIFPSFVGMVAIYVILLRIGGLDTLWGMILIYTA
                     GNVPYNTWLVKSYVDSVPRSLDEAARIDGASNARTYFQIILPLTKPIIVFLAISSFTG
                     PWMEYIFSKMILRSNENQTLALGLFSFVTEKKNEFTTFCAGALLVAIPFVFFFTLTQK
                     MLVTSLSAGALKE"
     gene            complement(978..>1580)
                     /locus_tag="PK898_10600"
     CDS             complement(978..>1580)
                     /locus_tag="PK898_10600"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU20516.1"
                     /translation="VGITAYLLGSISPSYLLGKMLKGIDIREHGSHNAGTANAISTIG
                     FLPGLVTMILDIGKGALSAWIGMRLAGKTGMQIGTVAVVAGHNFPILMHFRGGKGIAA
                     FIGASLISFPAAVAAYLLFSWIGIILSHYIAIGTLVAFTLLPFTGLWLTHGDISWFLT
                     LLAVSFLILLKHRPNFTAIRNRTEQKFYFKKRFETLLKRN"
BASE COUNT          426 a          366 c          356 g          432 t
ORIGIN      
        1 atttataact tcagaaggac aaaatcatac aaggaaggag agctgtgatg gtagagactc
       61 aatttgacaa gaaaatgaaa cgggaacgga taatcagtaa aatcaatacc ggcttggtat
      121 taatcttcct gattctggtg acattaatcg tgctgtatcc tctggtttat ttggtcagtg
      181 cggcgttctc accgggaaat tccatctcgt ccctcaatat tatcccgttc ggagacgggt
      241 ttacgatcga acactatcgc cggctcttcc gggaaaccaa ttacctgaat tggtttaaaa
      301 atacactcat tatcgccgcc ggaacttcaa tttcaacagt cgcggtttgt gccattgctg
      361 cctatgtctt ttcacggttt cgttttccgt ttaaaaaagg gatgatgctt ttactgttga
      421 ttttgcagat tttcccttct tttgtcggga tggttgcgat ttatgtgatt ctcctccgaa
      481 tcggcggatt ggatacgcta tggggaatga tcctcattta taccgccggc aacgtcccgt
      541 ataatacctg gctggtaaaa agttatgtcg attccgtacc tcgcagtctg gatgaagccg
      601 cacgaataga cggcgccagc aacgcacgaa cgtattttca gattatcctg cctctgacca
      661 aaccgattat agtgttcttg gcaatttcca gcttcaccgg accttggatg gaatatatct
      721 tttcaaaaat gattctgcgg tcgaatgaga atcagacgct ggcattggga ttgttcagct
      781 ttgtgaccga gaagaaaaac gaatttacta ctttttgcgc cggagcattg ttggttgcga
      841 taccgtttgt attctttttt acccttaccc agaagatgct cgtcacttca ttaagtgccg
      901 gagcgctgaa ggaataaaaa ccgttatcgg gcaggcaagg cggaaaacaa tcgttttttt
      961 cttgcctgct ttgacggtca gttccttttc aataaagttt cgaaacgttt tttaaaataa
     1021 aatttttgtt ctgtacggtt gcggatggcc gtaaagttgg gacgatgctt caataaaatc
     1081 aggaatgaaa cagccagaag cgtcaggaac caggaaatat ctccgtgggt caaccataag
     1141 ccggtaaacg gtaaaagcgt aaacgcaacc aatgtcccaa tcgcaatata atgcgaaagg
     1201 atgattccga tccaactgaa taataagtaa gccgccaccg ccgccggaaa ggaaattagg
     1261 gacgcaccga taaaagccgc gattcctttt ccccctctga aatgcatcaa tatcgggaaa
     1321 ttatggccgg caacaaccgc aaccgtaccg atctgcatgc cggtttttcc ggccagccgc
     1381 attccgatcc aggcggataa ggcgcctttt ccgatatcca gaatcatcgt aaccaatccc
     1441 gggagaaaac cgatggtcga aatcgcattt gccgttccgg cattatgcga gccgtgttcc
     1501 cgaatgtcga tccctttcaa cattttcccc agcagataag atggggaaat actccccagc
     1561 aaataggctg tgatcccgac
//
LOCUS       DAOSMM010000158         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_78657_length_1580_cov_8.537705, whole genome shotgun sequence.
ACCESSION   DAOSMM010000158 DAOSMM010000000
VERSION     DAOSMM010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78657_length_1580_cov_8.537705"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PK898_10605"
     CDS             complement(<1..1114)
                     /locus_tag="PK898_10605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU20517.1"
                     /translation="MDYLKKFSIPMSQPDITESEREAVNSVLRTTYLSMGPYLDEFEK
                     SLREFTGSAHAIAVSSGTAGLHLSVRAAGWQDNDWVITSPFSFVSSTNVLLYERITPI
                     FVDVDPMTGNIDPQQVEQAVHDLTSSRAAAQNWLPPKGVSDSGQLKGMIAIDVFGQPA
                     DFDTLRKIANSFHLKIIEDSCEALGAEYKNQKAGTLGDFGVFAFYPNKQITTGEGGLV
                     ITNHDEEADKIRAMRNQGRAPGDTWLEHTYLGYNYRMDELSAALGAAQMKRIQELMAA
                     RQRVADWYRAELSSVPGITCPVVAPTTTRSSWFVYVIRLDRGIDRKSVSEKLAALGIP
                     VRPYFSPIHYQPYIREKFGYQKGRFPVTEDLGERGLA"
     gene            complement(1124..1549)
                     /locus_tag="PK898_10610"
     CDS             complement(1124..1549)
                     /locus_tag="PK898_10610"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU20518.1"
                     /translation="MFKKNPSGPPQQAGPAIERVTSILGPGIIWRGNLRGSGGIRIEG
                     TFEGEIQIQGMVVIGETGRVTCNNLRANTVIVAGTVRGNITAEKLEIRSTGRVWGDVS
                     VVGFSTEEGAFLRGQVRMEDKVIIEPQPPSNSNLPYPSF"
BASE COUNT          404 a          398 c          343 g          435 t
ORIGIN      
        1 atgccaatcc cctttctccc agatcttccg taaccggaaa tctgcctttc tgatatccga
       61 atttttctcg aatataaggt tgataatgaa tgggcgagaa ataggggcgt accggaatcc
      121 ctaatgccgc taatttttcc gagaccgatt ttcgatcgat tccacggtcc aaccggatca
      181 cataaacgaa ccaactgcta cgagtggtgg tgggggcaac cactggacag gtaattccgg
      241 ggacagaaga aagctcggct cggtaccagt cagccactcg ttgacgggca gccattagtt
      301 cttggattcg cttcatctgg gcagctccca aggcagcgct taattcatcc atacgatagt
      361 tatatcctaa ataagtatgc tctaaccaag tatctcctgg ggctcgccct tgatttcgca
      421 tggcgcggat tttatcagct tcttcgtcat gattggtaat taccaaaccg ccttctccgg
      481 ttgtgatttg tttattagga taaaacgcga atacaccgaa atctcccagc gtccccgctt
      541 tttgattttt gtattcagcc cctaatgctt cgcaagaatc ctcaattatt ttcagatgaa
      601 atgaattggc gatttttctc aacgtatcaa aatcagctgg ttggccgaat acatcgatcg
      661 caatcatgcc tttcaactgc cctgaatcgg atactccttt cggcggtaac cagttttgtg
      721 ctgctgcacg agaagaagtc aaatcatgga cagcctgttc gacctgttga ggatcaatat
      781 tccccgtcat cggatccaca tcaacaaaaa tcggtgtaat tcgttcatat aagagaacat
      841 ttgttgaaga tacaaaagag aagggggaag taataaccca atcgttatcc tgccaacctg
      901 ccgcgcgaac acttagatga agtcctgcag tgcccgaact gactgcgatc gcatgtgctg
      961 atccggtaaa ttctcgcaaa gatttttcaa attcatctaa ataaggaccc atgctcaagt
     1021 atgtagtgcg caggacggaa ttaactgcct ctctttccga ttcggtaata tccggttgtg
     1081 acatcggaat gctaaatttt ttcaaataat ccatgtataa ctcctaaaag gaaggatagg
     1141 gtagattcga atttgaagga ggttgtggct cgataatcac tttatcctcc attcgaacct
     1201 ggcctcgtaa aaaggcgcct tcttcggtgg aaaatcctac gacgctgaca tctccccaaa
     1261 cccggccggt gctgcgaatc tcaagctttt cagctgtgat gttgccccgt actgtgccgg
     1321 caacgattac agtgtttgcg cgcagattgt tgcatgtgac tctgccggtc tctccgataa
     1381 cgaccatccc ctgaatttga atctcgcctt caaaggtacc ctcaattcga atccctccgc
     1441 ttccgcgcag gtttccccgc caaatgatac cgggaccaag aatagaagta acccgttcga
     1501 ttgccggacc ggcttgttgc ggcggaccgg atggattctt tttaaacatc aggcaatttt
     1561 cctatattct ttcggattcg
//
LOCUS       DAOSMM010000159         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_78658_length_1580_cov_8.523279, whole genome shotgun sequence.
ACCESSION   DAOSMM010000159 DAOSMM010000000
VERSION     DAOSMM010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78658_length_1580_cov_8.523279"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..457
                     /locus_tag="PK898_10615"
     CDS             32..457
                     /locus_tag="PK898_10615"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU20519.1"
                     /translation="MFKKNPSGSPQQAGPTIERVTSILGPGIIWRGNLRGSGGIRIEG
                     TFEGEIQIQGMVVIGETGRVTCNNLRANTVIVAGTVRGNITAEKLEIRSTGRVWGDVS
                     VVGFSTEEGAFLRGQVRMEDKVIIEPQPPSNSNLPYPSF"
     gene            467..>1580
                     /locus_tag="PK898_10620"
     CDS             467..>1580
                     /locus_tag="PK898_10620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU20520.1"
                     /translation="MDYLKKFSIPMSQPDITESEREAVNSVLRTTYLSMGPYLNEFEN
                     SLREFTGSAHAIAVSSGTAGLHLSVRAAGWGDNDWVITSPFSFVSSTNVLLYERITPI
                     FVDVDPMTGNIDPQQVEQAVHDLTFSRAAAQKWLPPKGVSDSGQLKGMIAIDVFGQPA
                     DFDALRTITDPFQLKVIEDSCEALGAEYKNRKAGTLGDFGVFAFYPNKQITTGEGGLV
                     ITNHDEDADKIRAMRNQGRAPGDTWLEHTYLGYNYRMDELSAALGAAQMKRIRELMAA
                     RQRVADWYRAELSSVPGITCPVVAPTTTRSSWFVYVIRLDRGIDRKSLSEKLEALGIP
                     VRPYFSPIHFQPYIREKFGYQKGRFPVTEDLGERGLA"
BASE COUNT          412 a          357 c          413 g          398 t
ORIGIN      
        1 cgaatccgaa agaatatagg aaaattgcct gatgtttaaa aagaatccat ccggttcgcc
       61 gcaacaagcc ggtccgacaa tcgaacgggt cacttccatt ctcggacccg gtatcatttg
      121 gcgcgggaac ctgcgcggaa gcggagggat tcgtatcgag ggtacgttcg agggtgagat
      181 tcagattcaa ggaatggtcg tgatcgggga gaccggccga gtcacttgta acaatctgcg
      241 cgcaaacacc gtaatcgttg ccggtacggt tcgaggaaat atcacagccg aaaagctcga
      301 gattcgcagc accggccggg tttggggaga tgtcagcgtc gtaggctttt ccaccgaaga
      361 gggcgctttt ttacggggtc aggttcgaat ggaagataaa gtgattatcg aaccgcagcc
      421 cccttcaaat tcgaatctcc cctatccttc cttttaggag ttatacatgg attatttgaa
      481 aaaatttagc attccgatgt cacaaccgga tattaccgaa tcggaaagag aggcagtcaa
      541 ttctgtcctg cgtaccacat acctgagcat gggaccgtat ctgaatgaat tcgaaaattc
      601 tttgcgagaa tttaccggat ccgctcatgc aatcgcggtc agttcgggca ccgctggtct
      661 tcatttaagt gttcgcgcag caggttgggg agataacgac tgggtaatta cctctccttt
      721 ttcatttgta tcttccacaa atgttctctt atatgaacga attacaccga tttttgtaga
      781 tgtggatccg atgacaggga atattgatcc tcaacaggtc gaacaggctg ttcatgattt
      841 gactttttct cgtgcggccg cacaaaaatg gttacctccg aaaggagtat ccgattcggg
      901 gcagttgaaa ggcatgatcg cgatcgatgt atttggccaa ccggctgatt ttgatgcatt
      961 gagaacaatc accgatccgt ttcagttgaa agtcatcgaa gattcttgcg aagcattggg
     1021 cgccgaatac aaaaatcgaa aagcggggac actgggagat ttcggagtct tcgcgtttta
     1081 tcccaataaa caaatcacta cgggagaagg cggtttggta attaccaacc atgacgaaga
     1141 tgccgataaa atccgcgcaa tgcggaatca gggacgcgct ccgggagata cttggttaga
     1201 acatacctat ttaggatata actaccgcat ggatgaatta agtgccgcgc tgggagcagc
     1261 ccagatgaag cgaatccggg aactgatggc cgcccgtcag cgggttgccg attggtaccg
     1321 ggctgaactt tcttctgtcc ccggaattac ctgtccggtg gttgccccta ccaccacccg
     1381 cagcagttgg ttcgtttatg tgatccggtt ggaccgtggg atcgatcgaa aatccctatc
     1441 ggaaaaatta gaggcattgg gaattccggt tcgtccttat ttttcaccta tccattttca
     1501 accctatatt cgagaaaaat tcgggtatca gaaaggcaga tttccggtta cggaagatct
     1561 gggagaaagg ggattggcat
//
LOCUS       DAOSMM010000160         1572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_79219_length_1572_cov_14.079103, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000160 DAOSMM010000000
VERSION     DAOSMM010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79219_length_1572_cov_14.079103"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1170
                     /locus_tag="PK898_10625"
     CDS             181..1170
                     /locus_tag="PK898_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20521.1"
                     /translation="MSDLLQVKGLETQFRTREGSVHAVNGVSFNLKEGETLGIVGESG
                     CGKTVSMMSLLGLIPMPPGKVVAGEALFEGKDLLKLSNEELRHIRGAKISVVFQDPMT
                     SFNPVLTIGKQTAEPYQIHRNAGKEEAYERAVKYLEMVGIPNAKERLNDYPHQFSGGM
                     RQRVMIAMGLICEPQILIADEPTTALDVTIQAQIIELMKRLRDELGMAIIWITHDLGI
                     IAGLAHRVNVMYGGYIVETAPVKSLYKEPQHPYTLGLLGSLPRMDEMSYRRLVSIDGL
                     PPTLLEKPTYCPFEPRCKYAVEKCRHSNPQLSQVEGEDHYAACWVNPKTGKEY"
     gene            1170..>1572
                     /locus_tag="PK898_10630"
     CDS             1170..>1572
                     /locus_tag="PK898_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU20522.1"
                     /translation="MAEKEVLLKVEDLYKHFPIHKGLFSGSHEKVHAVDGISFEIYKG
                     ETLGLVGESGCGKSTTGRTILQLYRPTSGSVFFEGEDLAKLKGEALRKKRRYVQMIFQ
                     DPYASLNPRMTVGQIIGEPMEIHNTYKGAEQL"
BASE COUNT          453 a          311 c          416 g          392 t
ORIGIN      
        1 aatttgatcc tcttgaattg acaattgggt atgaggtaat tatactttta gaatggtacc
       61 aacgcaaaat tttgaaagaa taccgatttt ttcctgaaca cctgtcttta taaggcggga
      121 tttacccata aaaagcgtta ggatcagaaa tgtacgaaat tttattaaag gatagtatac
      181 atgtccgatt tgttgcaagt caaaggcctt gaaacgcaat tccgaacccg ggaagggagt
      241 gttcacgcag tcaacggtgt ttccttcaat ttgaaagaag gagaaacact ggggattgtc
      301 ggcgaaagcg ggtgcggaaa aaccgtctcc atgatgtctc tattggggtt aattcccatg
      361 cctcccggga aagtcgtcgc cggcgaagcc ctgtttgaag gaaaagatct ccttaaatta
      421 agtaacgaag agctacgtca tattcgcgga gccaaaatca gtgtcgtctt tcaagatccg
      481 atgacatctt ttaatccggt tctgacgatc gggaaacaaa cggcagagcc gtatcagatt
      541 caccggaatg cgggaaaaga agaggcttat gagcgggcgg tgaagtattt ggagatggtc
      601 ggaataccca acgccaagga acgattgaat gactatcctc atcagttttc gggaggaatg
      661 cgccaacggg taatgatcgc gatgggatta atctgcgaac cgcagatatt aatcgccgat
      721 gaaccgacca cggctttgga tgtgacgatt caggctcaga ttatcgagtt gatgaaacgg
      781 ctgcgagatg aactcggcat ggcaattatc tggatcactc atgatttggg gatcattgcc
      841 ggcttggcgc atcgggtaaa tgtcatgtac ggcggctata ttgtcgagac ggctccggtt
      901 aagagtctgt ataaagagcc gcaacatccg tatactctcg ggctgttggg aagtttgccg
      961 cgaatggatg aaatgagtta tcgcaggttg gtttccatcg acggattgcc gccgactttg
     1021 cttgagaaac cgacttattg tccgtttgaa ccccgctgta aatacgcggt tgaaaaatgc
     1081 agacacagca atcctcaact gtcacaggtt gaaggagaag atcactatgc tgcctgttgg
     1141 gtgaatccga agactgggaa ggagtattga tggcggaaaa agaagttctc ttaaaagtgg
     1201 aggatcttta taaacacttc ccgatccata aagggttatt ttccggaagt catgaaaaag
     1261 ttcatgcggt tgacggaatc agttttgaaa tttataaagg tgagacactt ggattggtcg
     1321 gcgaaagcgg ctgcggaaaa tccactaccg gaaggactat tttacagctg taccggccta
     1381 cctccgggag tgtctttttt gaaggggaag atctggcaaa gctgaaaggc gaggcgttac
     1441 ggaaaaagcg ccgatatgtt caaatgattt tccaggatcc gtatgcaagc ctgaacccgc
     1501 ggatgaccgt cggacaaatt atcggtgaac ccatggaaat tcacaatacc tataaagggg
     1561 cggaacaatt aa
//
LOCUS       DAOSMM010000161         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_79348_length_1570_cov_10.087789, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000161 DAOSMM010000000
VERSION     DAOSMM010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79348_length_1570_cov_10.087789"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1570
                     /locus_tag="PK898_10635"
     CDS             <1..>1570
                     /locus_tag="PK898_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20523.1"
                     /translation="LGIFSLSQYLFPNSNRLAGLGRWSFFPVIICLFGIGLRLNLFFS
                     LRKSNTVSVSQSFLIIFAVFFLFLSLLPVLSSGFYWDDAAFSTAIPAMRISGVSIWQR
                     IKDEIISYSLRGRINPFATFQFLIFYLFPDVHVYKAILLILTIIDGLLFYKFNSELFK
                     NQIAALAILLLLPLCIQLRIYHDPMQGYYGLMQMMFAELMISLICFNRYLENRKKKYL
                     IISIIFFTIGLMSYEMFYPLLVLYFLLAWHSQKNRKQTLSSCIPFACIEAILLSVSFG
                     FRSYSAKMGVGAYSGTTFSLDIGKILSTWFHQSLAALPLSYQMAGNDATILKRLIPAS
                     EIFSQSFAAFVRGIVWPDIVSLIVFLFLWVEIRKRIHLPVISGFHWAFGCTLLFGSGL
                     VLALSEKYQNEILPGLGYLPVFFGYFGAAFLIFCLAANVYRLLKKVISADTILTFGIS
                     VFMVVFLLNQQSNRRSVALMNEEFLYPRQTGEAALQAGILKEADPQKEILVENNPFNL
                     WEQEWSGGSLRDDF"
BASE COUNT          354 a          324 c          347 g          545 t
ORIGIN      
        1 tattaggcat attttctctt tcgcaatatc tttttccgaa ttcgaatcgc ttagccggac
       61 tcggaagatg gagctttttc cctgtcatta tttgtctgtt cggcatcggt ttacggttga
      121 atcttttttt ctcgctccga aaaagcaata cggtttctgt aagccaatcg tttttgatca
      181 ttttcgccgt tttttttctg tttctcagtt tgcttccggt tctttcgagc ggtttttatt
      241 gggatgatgc agcattctcc acggcgattc cggcaatgcg aatttcggga gtgtcgatat
      301 ggcaacgtat caaggatgaa attatcagct actcgttacg agggcgtatc aatcctttcg
      361 ctacctttca atttttaatt ttttacctgt ttccggatgt tcatgtctat aaagcgatat
      421 tattgattct aacaataata gatggcttat tattttataa attcaattcc gaattattca
      481 agaatcaaat cgccgcttta gcgatcttgc tgctcttgcc gctctgtatt caacttcgaa
      541 tttatcatga tccgatgcag ggctactatg gactgatgca aatgatgttc gcggaattaa
      601 tgatctcctt aatctgtttt aaccgctatt tggaaaatcg gaaaaagaaa tatctgatca
      661 taagtatcat tttttttacg attggtttga tgtcctatga aatgttttat cccctgcttg
      721 tgttgtattt tttgctggct tggcattccc aaaagaaccg gaaacaaact ttgtcatcct
      781 gcattccttt tgcatgcatc gaagcgattc tcttgtcggt ttcattcggc ttccgttcct
      841 attcggcgaa aatgggagtg ggtgcttact cggggactac attcagtttg gacatcggaa
      901 aaatcttatc tacctggttt caccagtctc ttgccgcgct tccgctcagt tatcaaatgg
      961 cggggaatga tgcgacgata ttaaagcgct taattccggc atccgaaatt ttcagtcagt
     1021 cttttgcggc atttgtccgc ggcattgttt ggccggatat tgtttcgtta atagtatttt
     1081 tatttttatg ggttgagatt cgcaaacgga tccatttgcc ggttatctcc ggatttcatt
     1141 gggcattcgg ctgtacgctc ctgtttggat ccggtctggt actggctttg agcgagaaat
     1201 accagaatga aatacttccc ggcttagggt atttgccggt tttctttggc tattttggcg
     1261 ccgcctttct gatcttttgc ttagctgcca acgtatatcg gctcctgaaa aaagtgatct
     1321 cggcggatac gattctgact ttcgggatat ccgtttttat ggtggtattc ctgctgaatc
     1381 agcaatcaaa ccggcggtcg gtggctctga tgaacgagga attcctatat ccccgacaaa
     1441 cgggtgaggc agctttacaa gccggaattc tgaaagaggc ggatccgcaa aaagaaattc
     1501 tggtcgagaa caatccgttc aatttgtggg aacaagaatg gtccggagga tcgctgcgcg
     1561 atgattttat
//
LOCUS       DAOSMM010000162         1557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_80278_length_1557_cov_14.157790, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000162 DAOSMM010000000
VERSION     DAOSMM010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80278_length_1557_cov_14.157790"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1062
                     /gene="arcC"
                     /locus_tag="PK898_10640"
     CDS             109..1062
                     /gene="arcC"
                     /locus_tag="PK898_10640"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOU20524.1"
                     /translation="MAKKVAVVAIGGNSLIKDKDHQSCQDQYLAAKETSKHIVDMIQA
                     GWDVVIGHGNGPQVGFILQKAELAEKEIGLAMAPLDVCGAQSQGQIGYMLAQNLQNEL
                     KKRGIDKPVACVVEQTVVDKNDPAFQNPSKPIGGFMSEAEAKAKAKSEGWTVIEDAGR
                     GWRRVVPSPIPQEIVELEAIKTLIDAGVVTISVGGGGIPVIRNDKGELEGVAAVIDKD
                     FGSSLLARNIKADMLLISTAVEKVAINFGKPDEKWLDKMTLAEAKAYLSEGKHFKNGS
                     MAPKIQACIWFLEAGGKQALITNPENIGRALRGETGTLITA"
     gene            1124..>1557
                     /locus_tag="PK898_10645"
     CDS             1124..>1557
                     /locus_tag="PK898_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU20525.1"
                     /translation="MTWDHVIKNGTIVTPAETYKANLYIKDEKIAAITNEELPGEVKS
                     VTDAKGKYVLPGFIDTHSHSRDGRNGAHYKEDFFHSSMAGACGGITTILEMPNCNPAI
                     YNVANLNDLIEVITPKAHVDFGVWGLCLGNLNLDQILPLSEAG"
BASE COUNT          449 a          326 c          414 g          368 t
ORIGIN      
        1 tatttcagac aggctgccaa cggtgttcct gttcgaatgg ctttattagc cctcgtctgt
       61 ggctgcgaat aatcattaaa aaaacaaaag gaacgtagga atttatttat ggcgaaaaaa
      121 gttgcagtag tagcgatcgg tggaaattca ctgatcaaag ataaagatca tcaatcctgc
      181 caggatcaat atctggccgc aaaggaaacc tccaaacata ttgttgacat gatccaagcg
      241 ggatgggatg tcgtaatcgg tcacgggaac ggaccgcaag tgggttttat tctgcaaaaa
      301 gcagaactgg ccgaaaaaga aattgggttg gccatggcac cgttggatgt ctgcggtgcg
      361 caaagtcaag gtcaaatcgg ttatatgttg gcgcaaaatc ttcagaatga gttgaaaaaa
      421 cgcggtattg ataagccggt cgcgtgtgtg gtcgaacaga ctgtggttga taaaaacgac
      481 ccggctttcc agaatccgag caaacccatt ggcggtttta tgagcgaagc ggaagccaaa
      541 gccaaagcga aatccgaagg ttggaccgtg atcgaagatg ccggacgggg atggcggcgt
      601 gtcgttccgt caccgattcc gcaagaaatc gttgaactcg aagccattaa aacgctgatc
      661 gatgccggtg tcgtgaccat ttccgtcggc ggtggcggta tcccggtcat ccgaaacgat
      721 aagggtgagc tggaaggtgt cgcggcggta atcgataaag atttcggcag cagcttattg
      781 gcccggaata tcaaagccga catgcttctg atttccaccg cggtcgagaa agtggctatc
      841 aattttggaa aaccggatga aaaatggctg gataaaatga cgttggcgga agccaaagct
      901 tacctgtcgg aaggaaagca tttcaagaac ggatccatgg caccgaagat tcaggcttgc
      961 atctggttcc tcgaagcggg cggtaagcag gctttgatca ccaatccgga gaatatcgga
     1021 cgtgcattgc gcggtgagac cggtacgttg atcaccgcct gagatgataa agaatagttc
     1081 tgattatttt gatcagaact atttattttt taaggagaaa gctatgactt gggatcatgt
     1141 tataaaaaac gggacgattg tgacgcccgc tgaaacctat aaagcgaatc tttacataaa
     1201 agacgaaaaa atcgcagcaa taacaaatga agaacttccc ggagaagtga agtcggttac
     1261 cgatgcgaaa ggcaagtatg tactgcccgg ttttatcgat acgcattctc attcaaggga
     1321 cggccgcaac ggggcacatt acaaagagga cttttttcat tcatccatgg ccggtgcctg
     1381 tggcggaatt acgacaatcc ttgaaatgcc gaattgtaat ccggctatct acaatgtcgc
     1441 caacctgaat gatttgatcg aagtcatcac cccgaaagca catgttgatt ttggcgtttg
     1501 gggattatgc ctcggcaacc tgaatttgga tcagattctt ccgctttccg aagccgg
//
LOCUS       DAOSMM010000163         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_81479_length_1541_cov_7.614401, whole genome shotgun sequence.
ACCESSION   DAOSMM010000163 DAOSMM010000000
VERSION     DAOSMM010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81479_length_1541_cov_7.614401"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK898_10650"
     CDS             complement(<1..689)
                     /locus_tag="PK898_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20526.1"
                     /translation="MKKQITLNGNWTMRRQDQLETIPAKIPGSVYSTLLAAGKMDDPF
                     YRDNEMNALPLMEYDYSFSRPFEIPDELLNVDRLLLRCEGLDTLGTILINGEKIAETN
                     NMHRIWEFDISDTVHSGQNEIEIIFSSPTRFIRKAYEEDPYEGSSDAMRGFVHLRKAE
                     YMFGWDWGPRLPDAGIWRDISLIGIDHARFDNIYVRQEHRDGKVILTFDTELDLVDEL
                     PYEIRISVETPS"
     gene            complement(714..>1541)
                     /locus_tag="PK898_10655"
     CDS             complement(714..>1541)
                     /locus_tag="PK898_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU20527.1"
                     /translation="NIGVLFVDATASGLTHEFFSSILNSFKVEADHQGYVITFISNQI
                     NRNRVSLLEHCRHRNYDGVMIACVNFKNPEVVELVSSEIPTLSIDYEFPTCTSILSDN
                     LGGMKKLTDYVIQKGHRKLAIIHGEGTEVTHIRLDSFFQTLNENRIQIPKYFIKPALY
                     HEPLHSQIATRELLCLPERPSCIFYPDDYSLLGGLDELEQHHLRIPEDISIVGYDGIF
                     LSQILRPRITTMHQDTETIGKRAAEELINQIENPRTYKRHRVVVKQDLLEGETVKQL"
BASE COUNT          430 a          339 c          306 g          466 t
ORIGIN      
        1 gaaggagtct cgacacttat tcggatttca tagggaagct catccaccaa atccagttcc
       61 gtatcaaagg ttaagattac ttttccgtcc cggtgctctt gacgaacata aatattgtca
      121 aaacgggcat gatcgatccc gatcaacgag atatcgcgcc agattcccgc gtccggaagt
      181 ctcggacccc aatcccaacc aaacatatat tcggctttcc gtaaatgaac gaatccgcgc
      241 atggcatcac tagatccttc atagggatct tcttcatagg cttttcgaat aaatcgggtg
      301 ggtgaagaaa aaattatttc aatttcattt tgcccggaat gaaccgtatc agagatatca
      361 aattcccaga tgcgatgcat attatttgtt tcggcgattt tttcgccgtt gatcagaatt
      421 gttcctaacg tgtccaatcc ttcgcagcgc aggagcaatc gatcgacatt cagcagttca
      481 tcgggaattt cgaaaggtcg ggaaaacgaa taatcatatt ccattaaagg caaggcattc
      541 atctcattgt cccgataaaa cgggtcatcc attttcccgg cggctaaaag agtgctgtaa
      601 accgatccgg ggattttcgc cggaattgtc tctagctggt cctgccgtct catcgtccaa
      661 ttaccgttta atgtgatttg ttttttcata aattccgact ctccaaaagc gttttaaagt
      721 tgtttaacgg tctccccttc caataaatct tgtttcacaa ccacccgatg tcttttataa
      781 gttctcggat tttcgatttg attgattaat tcttcggccg ctctttttcc gatcgtttcc
      841 gtatcctgat gcatcgttgt aatacgggga cgtaaaattt gagaaaggaa tataccatcg
      901 tatccgacaa tgcttatgtc ttcgggaatt ctcagatggt gttgttccag ttcatccaaa
      961 ccgcccaaaa gtgagtaatc atccggataa aaaatacaac tcggtcgttc gggcaagcat
     1021 aataattcgc gggtagcaat ttggctgtga agtggctcgt gatataaagc gggtttaata
     1081 aaatattttg gaatctgaat tcgattttca ttaagtgttt ggaaaaagct atccaatcgg
     1141 atatgagtca cttccgtccc ttccccgtga ataatcgcca atttgcgatg ccctttttga
     1201 attacataat ctgtcagttt tttcatccct cccagattat cggaaagaat ggatgtgcag
     1261 gtcggaaatt cgtaatcgat agaaagagtg ggaatttcgc tactaaccaa ttccaccact
     1321 tccgggtttt taaagttgac acacgcaatc atgacaccgt catagtttcg atgcctgcaa
     1381 tgttccagta aacttactcg attacggtta atttgattgc tgataaaggt aatcacataa
     1441 ccttgatggt ctgcttcgac tttaaaacta tttaaaatag aagagaaaaa ttcatgcgtt
     1501 aaaccgcttg cagttgcatc gacaaaaagg actccgatat t
//
LOCUS       DAOSMM010000164         1496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_84984_length_1496_cov_3.543373, whole genome shotgun sequence.
ACCESSION   DAOSMM010000164 DAOSMM010000000
VERSION     DAOSMM010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84984_length_1496_cov_3.543373"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..>1496)
                     /locus_tag="PK898_10660"
     CDS             complement(217..>1496)
                     /locus_tag="PK898_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU20528.1"
                     /translation="ALEATPWIAWDGQVFPDYYAHLPEDKVPRGRCLVPKDPQTGKGV
                     ARGFELIRQLREAINQREIPILLNHRAKKLILNRKGEVVGLEAIDSENHIVTLRAKKA
                     VIFGSGGFTHSPEKRLNFLRGPVFGGCAVPTNEGDFIDIATSVGAKLNNTANAWWAPV
                     VLEEVLEHPSPPTDVFMVSGDSMIYVNKYGKRIVDEKAVYNEKTQAFFTWDPIKGEYP
                     NLMVFMIYDQRTADIWGNYPPAAAYPIPTKDTPSPYLIYGNTPEDLARAIEERLNKIA
                     SATGNFHLDQSFSENLKRTIKVFNQYAEQGIDPEFHRGEQPIDLAYNGPAAPDHNLPN
                     ITMYPIASEGPYYAIIIAGGTLDTKGGPKINKKAQVLNHEDMPIPGLYGAGNCIAAPA
                     AQAYWAGGGTIGPALTFGYIAADYAVQENIKEP"
BASE COUNT          391 a          352 c          304 g          449 t
ORIGIN      
        1 acccaatgcg atattgcccg gtatttcctg attcataatc ttcacctccg ttttaatata
       61 attttcttca aataaacaat gatcatttat taactttaga aagaatcgat taatctgttt
      121 attcagatat aaaagtctat taaaatctaa tatttaaaag ataaattgag caaatgaata
      181 tttaaaataa attcatttgt tcaattataa actgttttac ggctctttga tgttttcttg
      241 tacggcataa tcagccgcga tatagccgaa agtcagagcg ggaccgattg taccgccgcc
      301 tgcccaatat gcctgcgcag cgggcgctgc gatacagttt cccgcaccgt aaagtccggg
      361 tatcggcata tcttcatgat ttaatacttg ggctttttta ttaatcttcg gccccccttt
      421 ggtgtcaaga gtacctcctg cgataatgat cgcatagtac ggtccttccg acgcgatcgg
      481 atacatcgta atattgggta aattgtgatc cggggcagca gggccgttat atgccaaatc
      541 gatgggttgt tctccccgat gaaattccgg atcgattccc tgctcggcat attgattaaa
      601 aactttaatt gtcctcttaa gattttcgga aaagctttgg tcaagatgaa aattgccggt
      661 tgcggatgcg attttattca acctttcttc gatcgctcgc gcaagatctt ccggagtatt
      721 gccgtaaatc aagtaaggag acggagtatc cttggtcgga atcggataag cagcggcggg
      781 aggatagttt ccccaaatat ccgcggttcg ctgatcgtag atcatgaaga ccatcaaatt
      841 cggatattct cccttgatcg gatcccaagt aaagaaggct tgtgtttttt cattataaac
      901 cgccttctca tccacgattc ttttcccgta tttatttaca taaatcatgc tgtctccgga
      961 aaccatgaaa acatcggtcg gaggactcgg atgttctaaa acttcctcta aaaccaccgg
     1021 cgcccaccaa gcatttgcgg tattgtttaa ttttgctccg accgaggttg ctatatcaat
     1081 aaaatcacct tcattcgtcg gaacggcgca tcctccgaaa acagggccgc gcaaaaaatt
     1141 taatcttttt tccggactat gcgtaaaccc gccgcttccg aaaataaccg cttttttggc
     1201 tcgtaaggtg acaatatgat tctcggaatc gattgcctcc aacccgacta cttctccctt
     1261 acggttcagg ataagctttt tcgcacgatg gttaagaaga atcggtatct ctctttgatt
     1321 gatcgcttct cgtaattgac gtatcaactc aaatcctcga gctactcctt ttcctgtttg
     1381 aggatctttg ggaaccaagc atcgccccct cgggactttg tcttcgggca ggtgagcata
     1441 gtaatcggga aaaacctgcc cgtcccaggc gatccaagga gtcgcttcca gcgcac
//
LOCUS       DAOSMM010000165         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_86011_length_1483_cov_11.538515, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000165 DAOSMM010000000
VERSION     DAOSMM010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86011_length_1483_cov_11.538515"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PK898_10665"
     CDS             complement(<1..806)
                     /locus_tag="PK898_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20529.1"
                     /translation="MNLKIMFRSGRFVTGLIFVLIVLGYAIFYPIINPTNPKQDRIPN
                     PLYVETEKLRAYLEAEEYEKANQEIERLSEIEDESLQKILAEVKSALESGHIKKALSG
                     AKQIKKTHPLYKEFETLRITLGFDSSIPADEEAARAELQKLTAHHQSVLAVAELLKSG
                     DVSGIEAYTKEDQTLAQEIQPILESDPSTWAESAETLVKNHEETYKWILEIQKRLDTF
                     SNEEPLKALAGIQKTLLVPKNSPPSKTFPLGTDSFSRNIIVEMAYGARLSL"
     gene            complement(809..>1483)
                     /locus_tag="PK898_10670"
     CDS             complement(809..>1483)
                     /locus_tag="PK898_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU20530.1"
                     /translation="IQLPSLIIGWIIGNLLGAVAAYRKGIFDRIIFPLSLFFSSIPVF
                     VLALLMKYYLGVYAHWFPMQGAYAAELMPSATFTFYWSLFRHWQLPFLATCLVMIGGQ
                     AIGMRSMSLYELNADYVLYAKLLGIPENKITKYVFKNAVLPQITGLALSLGTMISGAL
                     ITEIVFSYPGIGTQLVNAIHNVDYTLISGCTLFISITALIANFTIEIIYGLIDPRIRA
                     IQMEEE"
BASE COUNT          418 a          332 c          312 g          421 t
ORIGIN      
        1 agggataacc gagcgccgta tgccatctca acgataatat ttcgtgaaaa agaatcggtc
       61 cctaaaggga aagttttact cggagggctg tttttgggaa ccaatagtgt tttttgaatt
      121 cccgccaacg ctttcaacgg ttcttcattg ctgaaggtat ccaatctttt ttggatttcc
      181 agaatccatt tgtatgtttc ctcatggttt tttaccagcg tctcggctga ttccgcccat
      241 gtggaaggat cggattccaa aatcggttga atttcctgcg ccaatgtttg atcctctttt
      301 gtataagcct caatgccgct gacatctccg gatttcagaa gctccgccac agccaaaacc
      361 gattggtgat gagcggtgag cttttgcagt tcagctctgg cggcttcttc atcagccggg
      421 atggaactgt cgaaaccgag agttattcgt aatgtttcaa attctttata taaagggtga
      481 gtcttcttta tttgcttagc cccggaaagg gcttttttaa tatgtccgct ttccaatgcg
      541 cttttgactt cagccaaaat cttttgcagt gattcgtctt cgatttcaga cagtctttcg
      601 atttcctgat ttgccttttc atattcttcc gcttccagat aggcacgcag cttttcggtt
      661 tccacgtaca gcggattggg aatgcgatct tgtttgggat tcgtcgggtt gattatggga
      721 taaaaaatcg cataaccgag gacaatcaat acaaaaatca accctgtaac aaaacgaccg
      781 gaacggaaca tgattttcag attcataatt attcctcttc catttgtatc gcccgaatcc
      841 tcggatcaat taatccgtaa ataatttcaa tcgtaaaatt cgcaattaag gctgtaatgg
      901 aaatgaacag cgtacacccc gaaatcaatg tgtagtccac attatgaatg gcattcacca
      961 attgcgtacc gattcccggg taactgaaaa ctatttccgt gataagagcg ccgctgatca
     1021 ttgttcccag agataaagcc aatccggtta tttgcggtaa tacggcattt ttaaagacat
     1081 atttggtaat cttattttcc ggaatgccca ataactttgc gtaaaggaca taatcggcat
     1141 ttaattcata gagcgacatt gatcgcatac cgatggcttg tcctccgatc atcaccagac
     1201 acgtcgccag aaacggaagt tgccaatgac ggaacagcga ccaataaaaa gtaaaggttg
     1261 cactgggcat taattcagca gcataagctc cctgcatcgg gaaccagtgg gcgtaaacgc
     1321 ccaagtaata tttcatcaac agcgccaata caaataccgg aatcgaactg aaaaagagag
     1381 aaagagggaa aataatccga tcaaaaattc cttttcggta agcggctacc gcacccagaa
     1441 ggttcccgat gatccaaccg ataatcagag aaggaagctg gat
//
LOCUS       DAOSMM010000166         1478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_86454_length_1478_cov_12.161630, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000166 DAOSMM010000000
VERSION     DAOSMM010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86454_length_1478_cov_12.161630"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1468
                     /locus_tag="PK898_10675"
     CDS             221..1468
                     /locus_tag="PK898_10675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOU20531.1"
                     /translation="MPSETQRVKVHLTALTYEGMALGHLPDGRVVFVAGGLPDEEVEI
                     EITGEKRDFVFARVIQILRPSVKRISPKCVHFGTCGGCHFQHLNYIDQLSVKRSVVEE
                     QLRRIGGIQNPVVGEVVPSPEIWQYRNTMQFHINSEGQICFRKAGGMETVPIHECYLP
                     AEPIRRLFPSLVFGPSDSGKRIEIRCDSDRNLMLIEEGHRIDLSDRLAGGSSPTTQQT
                     DSSEKVESRKKVLFMDILGVRFQVSVGSFFQVNLPVAEAMIRYLQNNLPLDSKTNLLD
                     LYCGAGLFSRFFAEKVAHIDAIELSTDAASDFAANLAGKGSVSFRQGAVEQVLPQFRI
                     RGGVAILDPPRSGLRPRALDAIVRIAPSLIAYVSCDPATLARDLRSLLAVGYRLERIT
                     PFDMFPQTYHVECVVLMTNVKNK"
BASE COUNT          364 a          337 c          378 g          399 t
ORIGIN      
        1 ggtcccgatg aactttgctt ccgagggaga aatatcttct ccgcagacgc gctatctcgg
       61 caaggtggct gaactgacag aacggattca taccgaatcg atcgcaacct ttggggtaaa
      121 ggtggaagac ggatggatct tggtcgcttt gtgaactcca gcatcgtgaa ataccaaaat
      181 ttatcaacaa tatataacat ttttgtttga agagaaacac atgccctccg aaacgcaaag
      241 agttaaagtc catttgaccg ccctgactta tgagggaatg gctctgggac atcttcccga
      301 tgggcgggtg gtttttgtcg caggcgggtt gcctgatgaa gaagttgaaa ttgaaattac
      361 cggggaaaaa agagattttg tcttcgctcg tgtgatacaa attttgcgtc cgtccgttaa
      421 acgcatctct ccgaaatgtg ttcatttcgg cacctgcggc ggttgccatt ttcagcattt
      481 gaactatatc gatcaacttt ccgtaaaacg atccgtcgtg gaagaacagt taagacgtat
      541 cggcggaatt caaaatccgg tggttggcga ggtggttccc tcgccggaaa tttggcagta
      601 tcgaaatacg atgcagtttc atatcaattc ggaagggcag atttgcttcc gaaaagcggg
      661 tggcatggaa acggtgccta ttcatgaatg ctatttgccg gcggaaccga ttcggcgcct
      721 ctttccgtcc ctcgtttttg gtccttccga ttccggcaaa aggatcgaaa tccgctgcga
      781 cagcgaccga aacctgatgc tcatcgaaga gggacatcgg atcgatcttt ctgaccggtt
      841 ggcgggtggc tcatccccca caacgcaaca aacagattca tctgaaaaag tggaaagcag
      901 aaagaaggtc cttttcatgg acattctagg tgttcgattt caagtcagcg taggctcttt
      961 tttccaagtc aatctaccgg ttgcggaagc catgatccga tatttacaga ataacctccc
     1021 cctggattcg aaaacgaatc tgttggattt atattgcggt gccggtttat tttctcgatt
     1081 ttttgccgaa aaggtagcac acatcgatgc gattgagctc tcaaccgatg ccgcttcgga
     1141 ttttgccgcc aatttggccg gcaaaggatc cgtttctttc cggcagggag cggttgagca
     1201 agttttaccg caattcagaa ttcgcggcgg agtggcgatt ttggatccgc cccgaagcgg
     1261 gcttagaccg cgagccttag atgcgatcgt tcggatcgct ccgtcattga tcgcctatgt
     1321 ttcttgcgat ccggcaaccc ttgcccgtga tttgcgttcc ttgctggcag tgggatatcg
     1381 gctcgaacgg ataacacctt ttgatatgtt tcctcaaacc tatcatgtgg agtgtgtggt
     1441 gttgatgaca aatgtaaaaa ataaataagc gtatgaaa
//
LOCUS       DAOSMM010000167         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_87318_length_1468_cov_9.236377, whole genome shotgun sequence.
ACCESSION   DAOSMM010000167 DAOSMM010000000
VERSION     DAOSMM010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87318_length_1468_cov_9.236377"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="PK898_10680"
     CDS             <1..1402
                     /locus_tag="PK898_10680"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU20532.1"
                     /translation="LSAFGMIFYLILVQKMSPATLDDQTFYIGLMFILIDGFILFFGN
                     AKKPSVAWLLVGLCLVETAWNADSTLQRYVPQYSLRTELVDFRERNLSKIRSVLPPEG
                     KFYRVEKTEIRTYNDAMLLGYPGIGNFSSVANLSQSTFLKKLGFDCYATWCTYQGRTI
                     FSDSLLGIRFIVSDDPLNYYSNYSEDIYENPYAFPPAFYSQSDFNTFPTLEPDDPILL
                     QNGILNALMGSDKTWFEEIDLQPASSENLKALSTDESRSVFEKIDPEQPASIRYLFTI
                     PDEQPYTLFIPNVSLNYDIWLNGEQIFDDNKNYTPFLIDLDKYQAGDKIEILIDLAKS
                     TDYSDEVRLFSLDLAEFANAAKIIQTNAPQYVFDGKTVFRFAINPSEQDRYLLTSVSY
                     DPGWQATIDGKKVPTVPVMGSLIGIKIPAGSKELILSFRPKGLKVGAGISLVSLILFL
                     MEVFLYGKKKQEPVSD"
BASE COUNT          421 a          307 c          295 g          445 t
ORIGIN      
        1 tctgtcggct ttcggaatga ttttctatct cattcttgtt caaaaaatgt caccggccac
       61 cctcgatgat cagacatttt atatcggtct gatgtttatt ttgattgatg gttttatttt
      121 gtttttcgga aatgcgaaaa aaccttcagt tgcatggctt ttggtcgggc tctgcctggt
      181 cgaaacagct tggaatgccg attcgactct gcaacggtat gttcctcaat attctctgcg
      241 cacggagttg gtcgatttca gggaacggaa tctgagtaaa atccgatctg tcttgccacc
      301 cgaaggaaaa ttttatcgtg tcgaaaaaac agaaatacga acttacaacg atgccatgct
      361 tttaggttat ccgggaatcg gaaattttag ttcggtcgcc aacctttccc aaagtacctt
      421 cctgaaaaaa ctcggatttg attgttatgc aacatggtgc acctatcaag gcagaacaat
      481 attttccgac tcgttgctgg gaattcgttt tatcgtttcg gacgatccat tgaattatta
      541 ttcgaattac agcgaagaca tatatgaaaa tccttatgct tttccgccgg ctttttattc
      601 ccaatccgat ttcaacactt ttcccacatt agaaccggat gacccgatct tattacaaaa
      661 cggaatatta aacgcgctta tgggatccga caaaacctgg tttgaagaga ttgatttaca
      721 accggcctct tcggaaaatc tgaaagcact ctccaccgac gaaagtcgat ccgtttttga
      781 gaagatcgat cccgaacaac ccgcctcaat ccgttatctt tttacgattc cggatgaaca
      841 accttacact ctgtttattc cgaatgtcag cctgaattac gatatttggc tgaacggaga
      901 acagatcttt gacgataata aaaattacac accttttctg atcgatttgg ataaatatca
      961 agcgggggac aaaattgaga ttctcatcga ccttgcaaaa tcaactgatt attctgatga
     1021 agtgcgatta ttctcattgg atctcgccga gtttgcaaat gcagcaaaaa taattcaaac
     1081 gaatgcccca cagtacgttt ttgacggaaa aactgttttc agattcgcta tcaatccttc
     1141 tgaacaagat cgatatttat taacatcggt ttcgtatgat cccggttggc aagcgacaat
     1201 tgacgggaaa aaagtcccga cggttccggt aatggggagt ttgatcggaa taaaaattcc
     1261 ggccggttcc aaagagttga tcctttcctt ccggccgaaa ggattaaagg tcggggcggg
     1321 catttctctg gtttcgctga tattgttttt aatggaagtg tttttatacg gaaagaaaaa
     1381 acaagagccg gtatcggatt gatcagactc ttctataatt caatcgaaag aatgtaatca
     1441 atatcattaa aaatcagtaa cagctctt
//
LOCUS       DAOSMM010000168         1436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_90200_length_1436_cov_10.988414, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000168 DAOSMM010000000
VERSION     DAOSMM010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90200_length_1436_cov_10.988414"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK898_10685"
     CDS             complement(<1..689)
                     /locus_tag="PK898_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20533.1"
                     /translation="MKKQFTLNGDWTMRRLDRSETIPATIPGSVYSTLLAAGKMDDPF
                     YRDNEMNALPLMEYDYSFSRPFEIPDELLNVDRLLLRCEGLDTLGTILINGEKIAETN
                     NMHRIWEFDISESLHSGQNEIEIIFSSPTRFIRKAYEEDPYEGSGDAMRGFVHLRKAE
                     YMFGWDWGPRLPDAGIWRDISLIGIDHARFDNIYVRQEHRYGKVILTFDTELDLVDEL
                     PYEIRISVETPS"
     gene            complement(714..>1436)
                     /locus_tag="PK898_10690"
     CDS             complement(714..>1436)
                     /locus_tag="PK898_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU20534.1"
                     /translation="VITFISNQINRNRVSLLEHCRHRNYDGVMIACVNFKNPEVAELV
                     SSEIPTLSIDYEFPTCTSILSDNLAGMKELTDYVIQKGHRKLAIIHGEGTEVTHIRLD
                     SFFRTLNEKQIRIPKYFIKPALYHEPLHSQIATRELLSLPERPTCIFYPDDYSLLGGL
                     DELEQHHLKVPEDMSIVGYDGIFLSQILRPRITTMHQDTETIGERAAEELIEQIENPR
                     TYKRHRVVVKQDLLEGETVQQL"
BASE COUNT          382 a          316 c          312 g          426 t
ORIGIN      
        1 gaaggagtct cgacacttat tcggatttca tagggaagct catccaccaa atccaattcc
       61 gtatcaaagg ttaagattac ttttccgtat cggtgctctt gacgaacata aatattgtca
      121 aaacgggcat gatcgattcc gatcaaagag atatcgcgcc agattcccgc atccggcagt
      181 ctcggacccc aatcccaacc aaacatatat tcggctttcc gtagatgaac gaatccgcgc
      241 atggcatccc ccgagccttc gtaaggatct tcttcataag cttttcgaat aaatcgggtg
      301 ggcgaagaaa aaattatttc aatttcattt tgcccggaat gaagcgattc ggagatatca
      361 aattcccaga tgcgatgcat attatttgtt tcggcgattt tctcgccgtt gatcagaatt
      421 gttcctaacg tgtccaatcc ttcgcagcgc aggagcaatc gatcgacatt cagcagttca
      481 tcgggaattt cgaaaggtcg ggaaaacgaa taatcatatt ccattaaagg caaggcattc
      541 atctcattgt cccgataaaa agggtcgtcc attttaccgg cggctaaaag agtactgtaa
      601 accgatccgg gaattgttgc cggtattgtt tccgatcggt caagccgtct cattgtccaa
      661 tcgccgttta atgtgaattg ttttttcata aatatcgact ccaaaaagct tttttaaagt
      721 tgttgaacgg tctccccttc caataaatct tgtttcacta ccacacgatg tcttttataa
      781 gttctcggat tttcgatttg ttcgattaat tcttcggccg ctctttcccc gattgtttcc
      841 gtatcttggt gcattgtggt gatgcgggga cgtaaaattt gagaaaggaa aatgccgtcg
      901 tatccgacga tgctcatatc ttcgggaact ttcagatggt gttgttccag ttcatccaaa
      961 ccacccaaaa gtgagtaatc atccgggtag aaaatgcagg tcggtcgttc gggcagggat
     1021 aataattcgc gggtggcaat ctgactgtga agcggttcgt gatataaagc gggtttaata
     1081 aaatacttcg gaatcctgat ttgcttttca ttaagagtcc ggaaaaagct gtctaatcgg
     1141 atatgagtta cttccgtccc ttcgccgtgg atgattgcca gtttgcgatg ccctttttga
     1201 atcacataat cggtcaattc tttcatcccc gccagattat cggaaagaat ggatgtgcag
     1261 gtcggaaatt cgtaatcaat ggaaagagtg ggaatttcac tgctgaccaa ttccgccact
     1321 tccggatttt tgaagttgac acatgcgatc atgacaccgt cataatttcg atgcctgcaa
     1381 tgttccagca aacttacgcg attgcggtta atttgattgc tgataaaggt aattac
//
LOCUS       DAOSMM010000169         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_94240_length_1394_cov_7.235997, whole genome shotgun sequence.
ACCESSION   DAOSMM010000169 DAOSMM010000000
VERSION     DAOSMM010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94240_length_1394_cov_7.235997"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1321
                     /gene="alr"
                     /locus_tag="PK898_10695"
     CDS             203..1321
                     /gene="alr"
                     /locus_tag="PK898_10695"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOU20535.1"
                     /translation="MNKVKYYASYLDINLDAIQHNCRICANKFRVPIMAVVKADAYGF
                     GALPVAKACAEAGVESFAVARIQEGIELREGGIRQDILVFSLQDEDEMQTAVRYGLTI
                     SLNRFDQIEPIAKLKRESGISPKAHLKIDTGMSRFGFLPEELPELIRILRENQINEIE
                     GIYSHYANIDDDPKDPLNSIQKQRFDCALKLLNQAGIQPRWIHFTNSAAAFSNPDSRY
                     TMVRLGNGLLGVNPFYYGEAPDFLMKALSWKSRLISVRRIPAGHGVGYAQHTFLSEDS
                     LIGVVPVGYGDGYHQAEGNKMLIHGQWVPVIGRVCADVSMVKLPEYYEVGEEVVLIGK
                     QGEKEISIEELAKLWHITRAAVTCGISSRVERRYQKSG"
BASE COUNT          420 a          244 c          331 g          399 t
ORIGIN      
        1 aaaaagcaat aaaatgtcag acaataaacc caattatgtg taattcgtct tatttaaaat
       61 atgctaaatg taaatataat attaaattac tcggaaattt gtcggcgaaa aatgtatgca
      121 caattaatac ttataaatta agtattaatg aaatatgaaa taggtgctcg attgataaga
      181 gtaaattgcg ggaaattgct ccatgaataa ggtaaaatat tatgcttcct atcttgatat
      241 taatttagat gctattcaac ataattgtcg aatttgcgca aataagttca gggttccgat
      301 tatggcggtt gtaaaagcgg atgcttatgg attcggtgcg ttgccggtgg cgaaagcttg
      361 tgctgaggcg ggcgttgaat cttttgcggt cgccagaatt caagaaggaa tcgaattgag
      421 ggaaggcggc attcggcagg atattttggt attttctctc caagatgaag atgaaatgca
      481 gaccgctgtc cgttacgggc tgacgatttc tctcaaccgt ttcgatcaga ttgaaccaat
      541 tgcgaaactc aaaagagaat cgggtatttc tccgaaggcg catttaaaaa ttgatacggg
      601 gatgagtcgt tttggctttt taccggaaga attgccggaa ttgattcgaa ttttaagaga
      661 aaatcagatt aatgagatcg agggtatata cagccattat gccaatatcg acgatgaccc
      721 aaaggatccg ttaaattcga ttcaaaagca gcgttttgat tgcgcattga aactattaaa
      781 tcaagctgga attcaacccc gctggattca tttcactaat tcggctgcag cattcagcaa
      841 tcccgattct cgttacacaa tggttcgcct cggaaacgga ttgctcggag tgaatccgtt
      901 ttattatgga gaagctccgg atttccttat gaaagcatta tcctggaaaa gccgattaat
      961 ttccgtgagg cgaattccgg cagggcatgg agttggatac gcacaacata cttttctttc
     1021 cgaagattcg ttgatcgggg ttgttccagt tggatatggg gacggatatc accaggctga
     1081 ggggaataaa atgttgatac acggacagtg ggttcctgta atcggtcgtg tctgtgccga
     1141 tgtcagtatg gtgaaattac ccgaatatta tgaggtaggg gaagaagtcg ttttaatcgg
     1201 aaaacaggga gagaaagaga tctcaatcga agaattggct aaactttggc acataactcg
     1261 agccgctgtt acatgcggaa tctcatcccg tgttgaaagg cgttatcaaa aatccggata
     1321 gattgaaatt tacagattta gccagcgttg tgcttccctt ggcaactcac gaataccggc
     1381 aattcgttcc gtgc
//
LOCUS       DAOSMM010000170         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_95783_length_1379_cov_16.354230, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000170 DAOSMM010000000
VERSION     DAOSMM010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95783_length_1379_cov_16.354230"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PK898_10700"
     CDS             <1..423
                     /locus_tag="PK898_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU20536.1"
                     /translation="SLISPRMYRKWAMPYEKRIIEAIHRKGAKAKLHICGNTSHFLSD
                     LPQTGADMIDADWMVDFKTAVDTFGDQASACGNFDPVSVMLNGTPETVRDAVNACIQV
                     ARPNTFIAAGCEVPKNTPVENLKMVAQTIQEAALNPAL"
     gene            complement(455..1270)
                     /locus_tag="PK898_10705"
     CDS             complement(455..1270)
                     /locus_tag="PK898_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU20537.1"
                     /translation="MNEIFSDQTLLETKKVCKTFGSGKNAVNAVIDVDFQIHQGEIIS
                     IVGTSGSGKTTLAKILLGLLKETSGEILYKGKPRLLKNQKERRAYWQNIQAIFQDPFS
                     TFNQFFRVDKILNDCLKLQGLEHISQKEKTEKMQDACKFVNLKYEELYNKYPFELSGG
                     QMQRLMIARIFMVKPNILIADEPTSMIDACSRSTILDMLMKLREEEGMTILFITHDLG
                     LAYYLSDTIYIMESGRIVEKGPAEDVILHPQDSYTKQLVSDVPKLHEEWVLSY"
BASE COUNT          374 a          304 c          300 g          401 t
ORIGIN      
        1 tctctgatca gtccgcgaat gtatcgaaaa tgggcgatgc cgtatgaaaa acggattatt
       61 gaggcgattc accggaaagg tgcgaaagca aaattgcata tttgcgggaa tacttcccac
      121 tttctgtccg atttgccgca aacgggtgcc gatatgattg atgcggattg gatggttgat
      181 tttaaaacgg cggtggatac ttttggcgat caagcttctg cctgcggaaa ttttgatccg
      241 gtttctgtta tgctgaacgg aacgcccgaa acagtccgtg atgccgtaaa tgcctgtatt
      301 caggtggcaa ggccgaacac ctttattgcc gcgggttgtg aagtacccaa aaatacaccg
      361 gtcgaaaatc tgaaaatggt ggctcaaaca attcaggaag ccgctctgaa tccggccttg
      421 tagatttcaa aagggaaaga aggaaagtgg gctctcagta gctgagaacc cactcttcat
      481 gtaacttggg aacatccgaa accaactgct ttgtatagct gtcctgcgga tgcaaaataa
      541 cgtcttcggc gggacctttt tcgacaatcc ggcctgattc cataatatag atggtatcac
      601 tcaaataata tgccaggccc aaatcatgag ttataaacag gatggtcatg ccttcttctt
      661 cccgcaattt catcagcata tccaagatgg tggaacggga acaagcatcg atcatagaag
      721 tcggctcatc agcgatcagg atattgggct ttaccataaa tattcgggca atcatcagcc
      781 gttgcatctg cccgccggaa agttcgaacg gatatttatt ataaagctcc tcatatttca
      841 gattgacaaa tttgcaggca tcctgcattt tttccgtttt ttctttttga gaaatgtgct
      901 ccaatccctg caatttcaga cagtcattca aaattttatc gacccggaaa aactggttga
      961 aggtagaaaa tggatcttgg aaaatcgcct gaatattttg ccaatatgca cgtcgttctt
     1021 tctgattttt cagcagccgc ggtttccctt tatacaggat ttctccactg gtttctttca
     1081 acagtcccag taaaattttc gccagcgtgg ttttcccgga gccggaagta ccgacgatag
     1141 agattatttc gccctgatga atttggaaat cgacgtcaat aaccgcatta acggcatttt
     1201 tccccgatcc aaaagtcttg cacacttttt tggtttccaa taacgtttga tcggaaaata
     1261 tctcattcat aatcattctc cgaatgctgt tttgcagctt tttttgcata tttttctctc
     1321 agctcggatt cgctgaaaat acaacgggaa taacgtccgt ttccgaaatg aattaatgg
//
LOCUS       DAOSMM010000171         1348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_99057_length_1348_cov_7.047951, whole genome shotgun sequence.
ACCESSION   DAOSMM010000171 DAOSMM010000000
VERSION     DAOSMM010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99057_length_1348_cov_7.047951"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..528
                     /locus_tag="PK898_10710"
     CDS             196..528
                     /locus_tag="PK898_10710"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOU20538.1"
                     /translation="MIEYKGYIGIVTYDSDAKLFHGDVVNTRDVITFQGTTVNEIEKA
                     FIESIDDYLGWCKEEGVAPEKPYSGKFNLRLSPELHKEIAITAKKMNISINNFVEKAI
                     QDELALIH"
     gene            614..853
                     /locus_tag="PK898_10715"
     CDS             614..853
                     /locus_tag="PK898_10715"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOU20539.1"
                     /translation="METKLTLKLDRAVIQAAKRYAEENERSLSGLVEDYFRNLSADHG
                     SSNKYSPLVEELSGVISDEDIAASDYTQYLGKKYE"
     gene            846..1268
                     /locus_tag="PK898_10720"
     CDS             846..1268
                     /locus_tag="PK898_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOU20540.1"
                     /translation="MNKRVFVDSDIILDLLGKREPFYRFAAELCTLADTGEVELVTTS
                     LVIANVYYLLRKAKGNDRARELLRKLRMLIGVVPVTEHTVDLALNSHFSDFEDGLQYY
                     TALECGIPILLTRNVRDYTEKGLVIQTAEEYLKGMNSV"
BASE COUNT          388 a          268 c          322 g          370 t
ORIGIN      
        1 tttggatggt tgcttctggg gctgatatta aatacgatta tcacagcgga ttcactacct
       61 aagggactgg ttcacgtgtt cgggtcaaac taaacaacgt gcgggctgtt tttcacagac
      121 ctcacccgaa aaagataact gacaagggag ccgtcaattc tgtaaagaga tttttggaaa
      181 atgcgggggt tacttgtgat tgagtataag ggatatatcg gaatagttac gtatgattcc
      241 gatgcgaaat tgttccatgg tgatgtagta aatacccggg atgtgattac ttttcaggga
      301 accactgtta atgaaattga gaaggctttt attgaatcaa ttgacgatta tctgggttgg
      361 tgtaaagaag agggagttgc ccctgaaaag ccgtactccg ggaaattcaa tttgaggctt
      421 tctccggaac ttcataagga aattgcaata actgcaaaaa agatgaacat ttccataaat
      481 aattttgttg aaaaggcaat acaagacgaa ttggctctga ttcattgagg ctgttatcaa
      541 agtgaacgat tgagattcag actcttgcct gtacgtgtaa aatgaattat cattatacgt
      601 acaggaggat tccgtggaaa caaaacttac cctgaaacta gaccgggcgg taatacaggc
      661 tgccaaacga tacgcagaag aaaacgaacg aagtctttcc ggactcgttg aggattattt
      721 ccgcaatctt tccgccgatc atggatcctc aaataaatac tcgccgttgg tagaagaatt
      781 gagcggtgtt atttcggacg aagatatcgc cgcatcggat tacacgcagt atctcgggaa
      841 aaagtatgaa taagcgggtc tttgttgatt ccgatatcat acttgacctt ttgggcaaac
      901 gtgaaccgtt ttatcgcttt gccgctgaat tatgtaccct tgccgatacc ggagaggttg
      961 aactggttac cacgtccctc gttattgcca atgtatatta ccttttgaga aaggcgaaag
     1021 gaaatgaccg cgccagggaa ttgttacgga agctccgcat gctcattggt gttgtgccgg
     1081 taaccgaaca tacagtagat ttggctctca attcacattt ttcggatttt gaagatggat
     1141 tgcaatatta caccgcgctg gagtgcggaa taccaattct tcttacaaga aatgtcaggg
     1201 actatacgga aaaaggactg gtcatccaga ctgcggaaga gtatttgaag ggaatgaatt
     1261 ccgtctgatc accccagcgg attcaccact tccacgccgc ggtataactc atcccttgtc
     1321 catcgctttc ccccggaaga tgctccgg
//
LOCUS       DAOSMM010000172         1330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_101062_length_1330_cov_8.569412, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000172 DAOSMM010000000
VERSION     DAOSMM010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101062_length_1330_cov_8.569412"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PK898_10725"
     CDS             <1..1056
                     /locus_tag="PK898_10725"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOU20541.1"
                     /translation="AESIPGFKLPVMQSLGLGNIDGMIGYEPAEHPRGSFARMAEHSA
                     GKDTTIGHWEMAGLYIPKPFPVYPNGFSREILDEFERRIGRGTLGNKPASGTQIIAEL
                     GDEHVATGKPIIYTSADSVFQIAAHEEVIPVDELYRMCQIAREILTGDNAVARVIARP
                     FVGKSGAYVRTANRRDFSLKPPRPTVLDHLKNAGYNVAGVGKIEDIFCGQGITFAVHN
                     ESNLDGMHHTEECMERFETGLIFTNLVDFDMKYGHRRDVTGYGNALIEFDQWLGSFLP
                     KMKDSDALFITADHGCDPTHEGTDHTREYVPLLIYGKEVESGVNYGTRTTFADTGQTI
                     ASIFRVEPVEDGVNLFA"
     gene            complement(1125..>1330)
                     /locus_tag="PK898_10730"
     CDS             complement(1125..>1330)
                     /locus_tag="PK898_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20542.1"
                     /translation="SASSFQHTIKVLASAAIGGMKDPLYGLKENVIIGKLIPAGTGFV
                     PGRFDHEDEIVSPSALISNLPTE"
BASE COUNT          344 a          278 c          363 g          345 t
ORIGIN      
        1 gcggagagta tccccggatt taaattaccg gttatgcaat cactcggctt gggaaatatc
       61 gacggaatga tcggttatga accggctgaa catcctcgcg gttcgtttgc ccgaatggcg
      121 gaacattccg ccggaaagga tacaacgatc gggcattggg agatggccgg gttatatatt
      181 ccgaaaccgt ttccggtata tccgaatgga ttcagcagag aaatattgga cgaattcgaa
      241 cggcgaatcg gtcgcgggac gctgggaaat aaaccggctt ccggaacaca aattattgcg
      301 gaactgggcg atgaacatgt cgctaccgga aaaccgatca tttatacttc tgcggacagt
      361 gtatttcaga tagctgcaca tgaggaggtg attccggttg acgaattgta ccggatgtgc
      421 caaattgctc gggagatttt gaccggagat aacgcggtgg cacgtgttat cgcccgtccg
      481 ttcgtgggga aatcgggtgc ttacgtccgg acggcgaatc gccgggattt ttctttgaaa
      541 ccgccgcgtc cgactgtttt agatcacctg aagaacgcgg gatataatgt agccggtgtg
      601 gggaaaatcg aagatatttt ctgcggacag gggatcacgt ttgcggttca taacgaatcc
      661 aatttggatg gcatgcatca taccgaagaa tgcatggaac gatttgaaac ggggttgatt
      721 tttaccaatc tggttgattt tgatatgaaa tacggacacc gccgggatgt taccggttac
      781 gggaatgctt tgattgaatt tgatcaatgg ttgggatctt ttctcccgaa aatgaaagat
      841 tccgacgcat tgtttattac tgccgatcac ggttgcgatc caacccacga aggaaccgat
      901 catacccgtg agtatgtgcc attgttgatc tatgggaaag aggttgaatc gggtgttaat
      961 tatgggacac gaacgacctt tgctgatacg gggcagacga ttgcaagcat tttccgggtc
     1021 gagccggtag aagatggggt aaatcttttc gcatagataa taataagcat aaaaaaacag
     1081 ctcgcaaatg actgcgagct gttttttttc ttttctaaaa tgatttattc ggttggcaga
     1141 ttggaaatca gagcactggg gctgacgatt tcatcttcat gatcaaagcg tcccggaaca
     1201 aatccggtcc cggcggggat taacttcccg ataatgacgt tttctttcag accgtaaagc
     1261 ggatctttca ttcccccgat cgccgcgctt gccagcactt tgatggtgtg ctggaacgaa
     1321 gaagccgaca
//
LOCUS       DAOSMM010000173         1329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_101172_length_1329_cov_11.613815, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000173 DAOSMM010000000
VERSION     DAOSMM010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101172_length_1329_cov_11.613815"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PK898_10735"
     CDS             <1..623
                     /locus_tag="PK898_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU20543.1"
                     /translation="LAVLLNKGINLTGFYRTLIFIPAVTMPAAIGLIWKWILNYEFGL
                     LNAIIRILGGQPQAWLSDPNYVLLSVSAVLIWADVSLRMIVFLAGLQNIPKSLYEAAE
                     IDGANNRTKFFKITLPMLSPTIFFVTLMEIIGVFQIFDFIYLMIPMNSSGMPAARSLV
                     SYFYEEAFVRNNRGYGSAITVILFVIIFIITMIQMKLQKKWVFTES"
     gene            626..>1329
                     /locus_tag="PK898_10740"
     CDS             626..>1329
                     /locus_tag="PK898_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU20544.1"
                     /translation="MERSQNFSIRKHHIGTHLVLILAVIISIVPFLWMILTSFKTYEE
                     SIRIPLTVWPAQWNLINYITVWNKFPFLNFYFNTFFVMVVVIIGELIISSMAAYAFAR
                     LNFPFKNAIFIFCLSLMMVPSQIFLIPQYDLMVKAGLNDTLIALILPRLFNVFSVFML
                     RQFFMTLPRELDDAAKIDGCGFFGIYYQILLPLLKPALVSVAILSGLSTWKDLLWPLI
                     VNNSMEKYTLSAGLAL"
BASE COUNT          333 a          275 c          273 g          448 t
ORIGIN      
        1 ttttggcggt tttactgaat aaagggatta acctgaccgg tttctatcgg acgcttattt
       61 ttattcccgc ggttacgatg cccgcagcca tcggtttaat ttggaagtgg attctcaatt
      121 atgaattcgg tttattgaat gcgatcattc gaatactcgg aggacaaccg caagcttggc
      181 tttctgatcc gaattatgtt cttttatcgg tatcggcggt gttaatctgg gcggatgtca
      241 gtttgcgaat gattgttttt ctggccgggc tgcaaaacat acccaaatcc ctctacgaag
      301 cagctgaaat tgacggagca aataaccgga caaaattttt taaaattaca ttaccgatgc
      361 tttctcccac gatatttttc gtgacactga tggaaattat cggggtcttc caaattttcg
      421 attttatcta tttgatgatt cccatgaaca gcagcggaat gccggcagcc agaagtttgg
      481 taagttattt ttacgaagaa gccttcgtcc gcaataacag aggatacggt tcggctatta
      541 cggtcattct gttcgttatc atttttatca ttaccatgat tcaaatgaaa ctccagaaaa
      601 aatgggtatt tacggagagt tagccatgga aagatcacaa aatttttcaa tccggaaaca
      661 tcatatcgga actcatttgg tcttaattct ggcggtaatt atctcgattg ttccgttctt
      721 atggatgatt ctgacttctt tcaagactta cgaagaaagc atccgaattc cattaaccgt
      781 gtggcccgct caatggaacc ttatcaacta tataacggta tggaataagt ttccttttct
      841 caatttttac ttcaatactt tctttgtgat ggtggttgtc atcatcggag aattgattat
      901 ctcttcgatg gctgcctatg cctttgcacg gctgaatttc ccttttaaaa atgccatatt
      961 tattttttgc ctgagcctga tgatggtccc gagccagatc ttcttgattc ctcaatatga
     1021 cctgatggtc aaagccggat tgaacgatac gttaatcgca ttaattctgc cgcgattatt
     1081 caatgttttc agcgtcttca tgttacgaca gttttttatg acgctgccgc gggaattaga
     1141 tgatgccgcc aaaatcgacg gctgcggttt tttcggcatt tattatcaaa ttcttttacc
     1201 gcttttaaaa cccgctttgg tttcggtggc gattttatcc ggactctcta cctggaaaga
     1261 cctgctttgg cctttgatcg tcaacaattc tatggaaaaa tacacgctgt cagccggatt
     1321 ggctctgtt
//
LOCUS       DAOSMM010000174         1321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_102079_length_1321_cov_10.439968, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000174 DAOSMM010000000
VERSION     DAOSMM010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102079_length_1321_cov_10.439968"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK898_10745"
     CDS             <1..416
                     /locus_tag="PK898_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOU20545.1"
                     /translation="LPIIPVLLIAFTILYLMRWRLNLLSLDDETALSISVNLKAERLI
                     FLICAVVVTSLCISLCGMINWIGMIVPHIARKIFGANTRYTVPATMLLGGLFMMICDD
                     FARVLFPAEIPLGIVTSIIGAVLFFPIMANNKVKA"
     gene            529..>1321
                     /locus_tag="PK898_10750"
     CDS             529..>1321
                     /locus_tag="PK898_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20546.1"
                     /translation="MKQKIFFVVCFLALFCLTFGSVFAEPVTITDILGREVTMEVPPA
                     LAVITGKKTSTITEVPFMFEYGKGHVAVPSGGQDGGKFISFLENPLPELKDATVESVA
                     AMKPDVIFLKSYTREDAGIAYEKTGIPVVYLSLESIDDYHKDVMNVGKIFGEEKRAEE
                     ILAYYDDIAKRVTEKASAIKEKKNVLLLQYSESNGAYSLKVAPAGWLQTTMVEMAGGE
                     PVWKEIGLNANSWTDVSFEQIAAWDPDVILVVNYFSDPKVSVEQLK"
BASE COUNT          347 a          244 c          335 g          395 t
ORIGIN      
        1 tattgccgat tattccggtg cttttgattg cttttacgat tctgtatttg atgcggtggc
       61 gtttgaatct cttatcctta gatgatgaaa ccgcattgtc aatcagtgtt aatttgaaag
      121 ccgaacggtt gatttttctg atttgcgctg tggttgtgac atcgctttgc atttcgcttt
      181 gcggcatgat taactggatc gggatgattg tgcctcatat cgcccggaag atattcggcg
      241 caaatacgcg ttataccgtt ccggcgacga tgttgttggg cggactattc atgatgatct
      301 gtgatgattt tgcacgcgtc ttgtttccgg ctgaaattcc tttgggtatt gtcacctcga
      361 tcatcggtgc cgtattgttt ttccccataa tggcgaataa caaggtaaaa gcgtgacggt
      421 ttctatgcaa cataactgaa tctttataaa aaaccattga cgtaaaacat actctgatct
      481 aaactttaat aaataaggat ttattaatta agtattatgg agggaaatgt gaaacaaaag
      541 atattttttg tggtttgttt tttggcgtta ttttgcctga cgttcggatc ggtctttgcc
      601 gaaccggtca caattacaga cattttaggt cgtgaagtaa ccatggaagt tcctccagcg
      661 ctggcagtga taacgggtaa aaagacttct accatcaccg aggtcccgtt catgttcgaa
      721 tacggaaagg gacatgtggc tgttccgtcc ggaggtcagg atggcgggaa atttatatct
      781 tttttggaaa atccgcttcc ggaactgaag gatgctacgg tggaatcggt ggcggccatg
      841 aaacccgatg ttatttttct gaaaagttac acgcgggaag atgctgggat cgcatatgaa
      901 aaaaccggta ttccggtcgt ctacctcagc ttggaatcga tcgatgatta tcataaggat
      961 gttatgaatg tagggaagat attcggagag gaaaaacggg ctgaagagat cttggcttac
     1021 tatgatgata ttgccaaacg tgtcacggaa aaggcctccg caatcaaaga aaagaaaaac
     1081 gtcctattgc ttcaatactc cgaatcaaac ggtgcgtatt cgttgaaggt cgctccggcc
     1141 ggttggttgc aaaccacgat ggtcgaaatg gccgggggag aaccggtatg gaaagaaatc
     1201 gggttaaatg ccaattcttg gacggatgtc agctttgagc agatcgctgc ctgggatccg
     1261 gatgtcattt tagtggtgaa ctatttttcc gatccgaagg taagtgttga acaattgaaa
     1321 a
//
LOCUS       DAOSMM010000175         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_102488_length_1317_cov_11.497623, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000175 DAOSMM010000000
VERSION     DAOSMM010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102488_length_1317_cov_11.497623"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PK898_10755"
     CDS             complement(<1..1058)
                     /locus_tag="PK898_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU20547.1"
                     /translation="MSRKVSILFVLTALLLAVVPVLAQDFQIPEIVEGKWNVAFVLIG
                     PINDGGWSQAHYDGAKYVSDNVDNVNVVYVENIPEGTESEQVFRSLAAKGFNVIFGTS
                     FGFMDPMEAVAEDFPDSYFIHIAGYKTNNTNFGNLMGAMEHMKFLAGYLSGQRCKMDG
                     EDTVGYMYTFPIPEELRLGNAFMMGVKKTCPECKMIVTPINSWHDPVVEQEAAKALFD
                     AGAYIVFTGADTPAPAIVAESYKEGKGKWGIPYDWKGSCTSKRCLTLPYWNWGPVYAS
                     IVEGLRDGTYTPGRHYFDGDENGLGLYGFMEGEELTDGLKDMPEEVLTFVRNYIADIQ
                     AGKWTRFDLFSGPIIDNQG"
BASE COUNT          367 a          317 c          267 g          366 t
ORIGIN      
        1 ccctggttgt caatgatcgg gccgctgaac agatcgaaac gagtccattt tccggcttga
       61 atatcggcga tataattgcg gacgaaagtc aacacttctt ccggcatatc tttcaagcca
      121 tctgtcaatt cttccccttc catgaagcca tagagaccga gaccgttttc atccccgtcg
      181 aaatagtggc gtcccggggt ataagtaccg tcgcgtaacc cttcaacgat tgaagcgtaa
      241 accgggcccc aattccaata tggcagggtg aggcatcgtt tggatgtgca ggaacctttc
      301 caatcgtacg ggattcccca ttttcctttt ccttctttat aggattcagc gacaatcgca
      361 ggggcaggtg tgtcagcacc ggtgaatacg atataagcgc cggcatcgaa cagtgctttt
      421 gcggcttcct gctccacaac cggatcatgc cagctgttga tcggggtgac aatcattttg
      481 cattccgggc aggttttctt cacacccatc atgaaagcgt tccctaagcg gagctcttcc
      541 ggaatcggga atgtatacat atagccgacg gtgtcttctc cgtccatctt gcatcgttgg
      601 ccactcaaat atcccgcaag gaatttcata tgttccatcg cacccatcag attaccaaaa
      661 ttggtgttgt tggtcttata gccggcaata tgaataaaat agctgtccgg aaagtcttcg
      721 gcaacagctt ccattgggtc cataaatccg aaggatgtac caaagatcac attgaagcct
      781 ttggctgcaa gactgcggaa gacctgttcc gattctgttc cttcagggat attttcgaca
      841 taaactacat tcacattgtc gacattatca ctgacgtatt tggcaccgtc ataatgcgct
      901 tgtgaccaac ctccatcatt gatcgggccg atcaaaacaa atgcgacatt ccatttgcct
      961 tcgacgattt ccggaatctg gaaatcctgg gcaagtaccg ggacgacagc aagcagtaaa
     1021 gctgtcaata cgaataatat agatactttt cgagacattt tttctccttt acagaaatat
     1081 agtttgattc ttccaacata gagactgtat gaacaacact atttatatta taaagtattt
     1141 aggttccgaa accgcataac ttaagatgac tgaatgagaa aattccatca agcggaattc
     1201 tctcctcgta aaccatgaat agtatttcag tctcataatc tgattatata aaatgacctc
     1261 aaaaaaagca ataaaatgtc agacaataaa cccaattatg tgtaattcgt cttattt
//
LOCUS       DAOSMM010000176         1280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_106942_length_1280_cov_16.373878, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000176 DAOSMM010000000
VERSION     DAOSMM010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106942_length_1280_cov_16.373878"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1280
                     /locus_tag="PK898_10760"
     CDS             107..>1280
                     /locus_tag="PK898_10760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU20548.1"
                     /translation="MSHKIRNFLFQYGVFIFFAVYLAIGLSVFDDYGCGPDEGMERQT
                     SLVNYKYVIEKLNLPVSEPVKTWLGYLPPLKEYRDRYYGTAVQIPMVLIESSSAFTME
                     PAEFYGIRHLYTFLNFFVAMICFYLLLKERFKHRGIALLGTAMLILTPRFFAESFYNN
                     KDVLFLSWYIISIYFIFRWFRKKTTGAAIVAGILLAFTINTRLNGIVLIPLVILFAVI
                     EQFEKKYPFREAMLPLCILIVTCLVTFFIITPNYWESPVSTFLEAFAFNMKHPNHGSD
                     RNLFFGNLVDSTKIWYFIPTWIVLTVPTCIVLLSVLGFGKIIMGFIRNRFSFFEHDEF
                     FMDLFMTAAGLIPLLVIIFMRVIIYNGWRHCYFFYGTLIYLAVVGLQTVWEIKIKHP"
BASE COUNT          315 a          287 c          249 g          429 t
ORIGIN      
        1 gggtgagggt gaaaatttca attttatgca gccgtgttac ggtacgccgg gttcaaaaaa
       61 atatgacacc aacgattcga ttaccccgtg cattataatg ctaggcatga gccacaaaat
      121 cagaaatttt ctcttccagt atggggtttt catttttttt gcggtctatc tcgcaatcgg
      181 attgtcggtt tttgatgatt atggctgcgg tccggacgaa ggaatggaac gccaaacatc
      241 tttggtcaat tataaatatg tcattgaaaa attaaatctt ccggtcagtg aaccggtaaa
      301 aacctggttg ggatacctcc cgcctttgaa agaatatcgc gatcgttatt atggcaccgc
      361 tgttcaaatt ccgatggttc tcatcgagag ttcttccgcc tttaccatgg aacctgccga
      421 attttatggt atccgccacc tgtacacttt tctcaatttc tttgtcgcca tgatttgctt
      481 ttatttgtta ttaaaagaac gcttcaagca ccgaggaatc gcacttttag ggacggcaat
      541 gctgatcttg actccccgtt tttttgccga gagcttttac aacaataaag atgtattgtt
      601 cctttcttgg tatatcattt cgatctactt catttttcgt tggtttcgca agaaaacaac
      661 cggcgcggct atcgttgccg gtattttact tgctttcacg atcaacaccc gcttaaacgg
      721 aatcgtgctc atcccgttgg ttattctttt cgccgtcatt gaacaattcg aaaaaaaata
      781 tccgttccga gaagccatgc tccctttgtg catcctgatc gtcacctgtt tagtcacctt
      841 tttcatcatt acgccaaact actgggaaag tcccgtttcc acttttctcg aagcctttgc
      901 attcaatatg aaacatccca atcacggctc ggaccggaat ttattcttcg gcaacttggt
      961 tgattcaacc aaaatatggt attttattcc aacatggatt gtcctgaccg ttccaacctg
     1021 tatcgttctt ttatccgtct tgggattcgg aaaaataatt atgggtttta tccgaaatcg
     1081 cttttcgttt ttcgaacatg atgaattctt catggattta ttcatgacag ctgccggact
     1141 gatccccctc ttggtcatca ttttcatgcg agtgatcatt tacaacggtt ggaggcattg
     1201 ttatttcttt tacggtacac tcatttatct ggcagttgtc ggtcttcaga cggtttggga
     1261 aataaaaatc aaacatccga
//
LOCUS       DAOSMM010000177         1274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_107721_length_1274_cov_11.055783, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000177 DAOSMM010000000
VERSION     DAOSMM010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107721_length_1274_cov_11.055783"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK898_10765"
     CDS             <1..407
                     /locus_tag="PK898_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU20549.1"
                     /translation="YITKPFDFDVLLAHVKAVLRRRENPVPEPEENILQVGDLKLDFP
                     QHKAEIGGKELELSPKEFELLTAMALNADHIISTDNLLKRVWGAEYTGDPQVLYVHIR
                     WLRSKIEEDPDHPKRILTVRSVGYKFNSTLSE"
     gene            404..>1274
                     /locus_tag="PK898_10770"
     CDS             404..>1274
                     /locus_tag="PK898_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20550.1"
                     /translation="MKNLRLQFILSHTLPVLITIPLVGIALSYAMETHLILKDMKQTI
                     QDQAALITQYSEGNPDVLKEEQAAEQMIQEIRPNLVTGVILFDHAWNVLATTDEDKNK
                     ITQRFPPPMMEMEFGQSGLEPVINISKYNPFKHSEDIIEMMVPVTNSDNQLLGMIRLN
                     FPVTYFEEQLKQTSDRIILILICGVILGVLFGLINAISLEKRLSRTTGAIYDLSLGIR
                     NNPLPEEGPEEMQKLAKAFNSLSQKLDSSEQTRTKLVSYLTHELGRPLGGLSSAVDAL
                     QMGAAQDEKLVQDL"
BASE COUNT          368 a          280 c          290 g          336 t
ORIGIN      
        1 attatataac gaaacccttt gatttcgacg ttctcttagc ccatgtgaaa gcggttttac
       61 gccgccgcga aaatccggtc ccggaaccgg aggaaaatat tttgcaggtg ggagacttaa
      121 aattggattt tccccaacat aaagccgaaa taggcggaaa agaactggaa ttatccccta
      181 aagaatttga actgttaacc gccatggctt tgaatgccga tcatattatc agtacagaca
      241 acttactcaa gcgagtttgg ggggcggaat ataccggcga tcctcaggtc ctttatgttc
      301 atattcgctg gctacgcagt aaaattgaag aagatccgga tcatcccaaa cgaatattga
      361 cggtccgcag cgtggggtac aaatttaatt cgactttatc tgaatgaaaa atcttagact
      421 tcaatttatc ctgagccata cattgccggt ccttattacg attcctttgg tgggtatcgc
      481 tttatcctat gccatggaaa cccacctgat cttgaaagat atgaagcaaa ccattcagga
      541 tcaggcagca ttaatcacac aatattccga agggaatcct gatgttctga aagaagagca
      601 agccgccgaa cagatgattc aggaaattcg tcccaacttg gttacgggag tcattttatt
      661 cgatcacgct tggaatgtgc tagcaaccac agacgaggat aaaaacaaaa ttacacaacg
      721 attcccaccg ccgatgatgg agatggaatt cggacaatcc ggtctcgaac cggtgatcaa
      781 tatttccaaa tacaatcctt tcaaacattc cgaagatatt atcgaaatga tggttccggt
      841 aaccaacagc gataatcagc tcctcgggat gatccgtttg aattttccgg tgacttattt
      901 cgaagaacaa ttgaaacaaa cttcggatcg aatcattttg attttgatct gcggtgtcat
      961 tttgggtgtg cttttcggtt tgatcaatgc gatcagcctt gaaaaacggc tcagccggac
     1021 gaccggcgcc atttatgatt tgtctctcgg cattcggaat aacccgctgc cagaggaggg
     1081 accggaagag atgcaaaagt tggcgaaagc gttcaattcg ctttcccaaa aattggattc
     1141 ttccgagcaa actcgtacaa aattggtttc gtatctgact cacgaactgg gacgcccgtt
     1201 gggcggactt tcatcagctg tggatgccct tcagatgggt gcggcacagg atgaaaagct
     1261 ggttcaggat ttga
//
LOCUS       DAOSMM010000178         1272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_107990_length_1272_cov_3.747740, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000178 DAOSMM010000000
VERSION     DAOSMM010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107990_length_1272_cov_3.747740"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..937
                     /gene="cysK"
                     /locus_tag="PK898_10775"
     CDS             2..937
                     /gene="cysK"
                     /locus_tag="PK898_10775"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015384397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOU20551.1"
                     /translation="MKIYHSVTELIGHTPLLELSRYETVHNLSATILAKIESFNPTGS
                     VKDRPALAIINRAEADGLLKPGSVIIESTSGNMGIGLASIASARGYRLILTMPDTMSV
                     ERRQLLKAYGAELVLTEGKLGMAGAVKKAEELAAAIPNSFIPNQFNNPENPLSHQSST
                     GPEIWEDTGGKIDIFVAGVGTGGTISGVGKYLKSKNPNMKVIAVEPAGSPVLSEGKAG
                     AHQIQGIGAGFVPNTLDTAIYDEIIPVQDNDAFATGKEITRLEGLLVGISSGAALWAA
                     TRVAVRPENDGKVIVTLFPDTGDRYLSGPMFSEAV"
     gene            953..>1272
                     /locus_tag="PK898_10780"
     CDS             953..>1272
                     /locus_tag="PK898_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20552.1"
                     /translation="MIKGTTNPTNLDQISIRIAENYASSDLPLYGASLRLPERKTVIE
                     LLLDFQKLLFPAYFGDPTLIRLPPEHYTHLLLERIRINLRQQIELAMEESSENSKRAQ
                     QICK"
BASE COUNT          373 a          302 c          297 g          300 t
ORIGIN      
        1 aatgaaaatt tatcactctg ttacggaact aatcggtcat acaccgttat tggagctgtc
       61 ccgttatgaa actgtccaca acttatccgc cacgatttta gcgaaaatcg aaagttttaa
      121 tcccaccgga agcgtcaaag accgtcccgc tctggcaata atcaatcgag ctgaagcaga
      181 tggattgctg aagcccggtt cagtaattat cgagtcgacc tccgggaata tgggaatcgg
      241 acttgcttcc attgcatcag ccagaggata tcggttaatt ttaaccatgc cggatactat
      301 gagcgtcgaa cgacgtcaac tgctgaaggc atacggagcc gaattggtcc taacggaagg
      361 aaaacttggc atggcgggag ccgttaaaaa agcagaagaa ctggctgctg ccattcccaa
      421 cagctttatc ccgaatcaat tcaataaccc ggaaaatccg ttgagccatc aatcctccac
      481 cggaccggaa atatgggaag ataccggtgg aaagatcgat atctttgtcg cgggagtcgg
      541 aaccggaggg acgatatcgg gagtgggaaa atacctcaaa tcaaaaaacc cgaacatgaa
      601 agttatcgca gtcgaaccgg ctggttctcc cgttttatcg gaagggaaag cgggggctca
      661 ccagatacag ggtatcggtg ccggatttgt tccaaataca ttagatacag cgatttatga
      721 tgaaatcatt ccagtccaag acaatgatgc ttttgcgacc ggcaaagaaa tcacccgttt
      781 ggaaggactg ctggtcggga tttcatccgg agcggcactt tgggctgcga cccgagttgc
      841 cgttcgaccc gaaaatgacg ggaaagtaat tgtcacttta tttccggaca ccggcgatcg
      901 ttatctttcc gggccgatgt tttcggaagc agtataacca atagaggcca aaatgataaa
      961 aggcactacc aaccccacca atttggatca aatttctatc cgaatagccg agaattatgc
     1021 cagttcggat ttacctttgt atggagcttc tctccggtta ccagaaagga aaaccgtaat
     1081 cgagctattg cttgattttc aaaagttact attccccgct tatttcggtg atcccacctt
     1141 gattcggtta ccgccggaac attacaccca tttattactg gaaagaattc ggatcaacct
     1201 caggcaacaa atcgaactcg ccatggaaga aagctcggaa aactccaaac gcgcacagca
     1261 gatttgtaaa ca
//
LOCUS       DAOSMM010000179         1263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_109091_length_1263_cov_11.644868, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000179 DAOSMM010000000
VERSION     DAOSMM010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109091_length_1263_cov_11.644868"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PK898_10785"
     CDS             complement(<1..323)
                     /locus_tag="PK898_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20553.1"
                     /translation="MVPPQNSEGINKPGRFHFIPWVIFLVAILFFIGTAIKTSPYRLL
                     RPYSVITARHLISTQSQLSDDKSRWLVLIPAAQSGSESDFWIDQIPITIAAYMKCVTE
                     EKCTPP"
     gene            complement(343..936)
                     /locus_tag="PK898_10790"
     CDS             complement(343..936)
                     /locus_tag="PK898_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20554.1"
                     /translation="MRKKLLLLFITIFFCSSCGRLYTEEQVNETVVAKIKEVAAMQKV
                     NVTIETRIIKETVLVTVAPEPTTTMIPLPYSTVTPLSFERTSIEDILAALSAAGIKVS
                     RFYYTPGSDEIGVLADWISQAMKFTIKDGAVEATGYAYSFPNPENLDKAYTYLTSQKA
                     GSSNKSIFRKNNILLEIDPDTPDNVLEKIKNCIETIP"
BASE COUNT          331 a          254 c          320 g          358 t
ORIGIN      
        1 ggaggagtac atttttcttc ggtcacgcat ttcatataag cggcgattgt gatcggaatt
       61 tgatctatcc agaaatccga ctccgagccg gattgagcgg ccggaatcaa aacaagccac
      121 cgacttttat cgtcggatag ctgcgattga gtcgagatca aatgccgtgc ggtgattacg
      181 ctataaggac gcaatagacg atagggggac gttttgatcg ccgtcccgat aaaaaaaaga
      241 atcgccacaa gaaaaatgac ccaagggatg aaatgaaaac gcccgggttt atttatcccc
      301 tccgaatttt gaggcggtac cacggattcc gagtgattct gattagggga ttgtttcgat
      361 gcaatttttg attttttcca gaacattgtc gggggtgtcc ggatcgattt cgagtaggat
      421 gttgttttta cgaaatatgg atttgttcga tgatcccgcc ttttggctgg tcaaataggt
      481 ataggcttta tccaaatttt cgggattcgg gaatgaatag gcataaccgg tcgcttcaac
      541 ggctccgtct ttgattgtga atttcatggc ttgggaaatc cagtcggcca acaccccgat
      601 ttcgtctgac cccggcgtgt aatagaacct gctgaccttt atgccggcgg cagataacgc
      661 ggcgaggata tcctcgatcg atgttctttc gaagctgaga ggtgtaaccg tggaataagg
      721 gagcggaatc atggttgtcg tcggttcggg agcaaccgtg acaagtacgg tttccttaat
      781 aatgcgagtt tcgatggtta catttacctt ttgcatcgcg gcgacctctt taatttttgc
      841 cacgacggtt tcattaacct gctcttccgt atagagtctg ccgcaggatg aacaaaagaa
      901 aatggttata aaaagaagaa ggagcttttt tcgcatattt ctccgtttgt aattttcaat
      961 tatcccatga ttttaaggaa aagaagaaaa ttcgtcgcca gagcattccg gaaagacatg
     1021 ttgtatcggg atatcgcgaa tgctgttttg tatggaaatg ccattcgctt tacaaatttt
     1081 tgccaacaat ctcaggaggt ttaccgagga taatcagatt atgtttatcg gaggatgatc
     1141 gagaggatat ttactatcaa caaataagag tacagttgat cgatttcaaa ttgttccatc
     1201 gtaggggacg gcgtccttgc gaagcacacc gaggttacga ggtgctgacg tcccgcgccg
     1261 agt
//
LOCUS       DAOSMM010000180         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_110859_length_1250_cov_7.071967, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000180 DAOSMM010000000
VERSION     DAOSMM010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110859_length_1250_cov_7.071967"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PK898_10795"
     CDS             <1..1029
                     /locus_tag="PK898_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU20555.1"
                     /translation="VWGLCLGDLNLDQIFPLSEAGVIGFKLFWGYAVDSRNYQLIYNY
                     EKGMQNVVPPLNDGEIYRIFRQVARTGKTLGIHAENFYLINTLIEEIRATGADDYAAL
                     LNSRPPVSEKMVITAAIRFARELGTKIRIMHLSNGESVDLIRQAGREHLPVSAEACPH
                     YLFLSDEDFNRCGSLMKSYPLVRTKHDQQMLWEGIRDGTISAVSSDHAPHTYEEKLLD
                     VWTSPAGVTNIETMGALMLTAVNRGWLRINDIVRLMSENPAKTFGLYPRKGTIRIGAD
                     ADILLVDLDREYTIDQEKLHSRVKHSPYHGMNGKGRVEKTILRGKTVAEDGEIAGEPI
                     GRFIKPPA"
BASE COUNT          338 a          280 c          313 g          319 t
ORIGIN      
        1 gtatggggcc tctgtctcgg agatttaaac cttgatcaaa tctttccgct ttccgaagcc
       61 ggtgtaatcg gatttaaatt attttgggga tatgcggttg acagccggaa ttatcagttg
      121 atttataact atgaaaaagg catgcaaaat gttgttcccc ctctgaacga cggagaaatc
      181 tatcggattt tcaggcaggt cgcccgaacc ggaaagacat tgggaattca cgccgaaaat
      241 ttctatctga tcaataccct gatcgaagag ataagggcta cgggtgccga tgattatgcg
      301 gcactgctca attcccgtcc gccggtttcc gaaaaaatgg tcattaccgc cgcgatccgc
      361 tttgcacggg aactcgggac gaaaatccgc atcatgcacc tttcgaacgg ggaaagcgta
      421 gatttaatcc gccaagccgg acgagaacat ctgcccgttt cggccgaagc ttgcccgcat
      481 tacctgtttt taagcgacga agattttaat cgttgcggtt cgctgatgaa atcttatcct
      541 ttggtgcgga cgaaacacga tcaacaaatg ctttgggaag ggattcgcga cggcactatc
      601 tccgccgtca gttccgatca tgcccctcat acctatgaag agaagctgct cgatgtttgg
      661 acctctccgg ccggtgtgac caatatcgaa acgatgggag cgctgatgtt gacggcagtc
      721 aacaggggtt ggttgcgaat caacgatatt gtccgattaa tgtcggaaaa tccggcgaag
      781 acctttggct tatatccgcg gaaagggact attcggatcg gagcggatgc cgatatcctg
      841 ttggttgatc tcgatcggga atataccatc gatcaggaaa aattgcattc ccgggtgaaa
      901 cattcgccgt atcacgggat gaacggaaaa gggcgggtag agaaaacaat tctgcgagga
      961 aaaaccgttg ccgaagacgg ggaaatcgcc ggtgagccta tcggacgatt tatcaaaccg
     1021 ccggcatagc ccgaccggac attcaatttg tccggttttg ccgattcagc ccatgagagt
     1081 tgcgtataga taccaaccct gcaaatagga atgaaatcat ttgatatggc cgcccggtta
     1141 cttatgagta ttttgtcccg atcaggtcat aaaaaatgat cattttttat aaaaacagat
     1201 tgaattctga tatgattttg accattcaaa ttttagcaaa gtaaagtttt
//
LOCUS       DAOSMM010000181         1245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_111484_length_1245_cov_16.626891, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000181 DAOSMM010000000
VERSION     DAOSMM010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111484_length_1245_cov_16.626891"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>1245)
                     /gene="murQ"
                     /locus_tag="PK898_10800"
     CDS             complement(124..>1245)
                     /gene="murQ"
                     /locus_tag="PK898_10800"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOU20556.1"
                     /translation="AERLKPSKVMRIEELGIASDAKEATAFALMAYETWHRRIGNIPS
                     ATGAKHPVIMGNITWAPPKRENISENIGPTEARNPATADMDRVSTLALVQEINEEDQK
                     VAGAVAAVLPQIAAAVDAIVSRMKQGGRMIYIGAGTSGRLGVLDASEVLPTFGVSPDW
                     VIGKIAGGSDALTKSIENAEDDPVLGASEIIELNVTEKDCVIGIAASGNTPYVLGALE
                     EAQSRKALTIGVACNPDGKIIRFCDIAILPVVGPEALTGSTRMKAGTAQKMVLNTLST
                     AVMVRLGKTYSNLMVDVQPSNQKLIDRQRRIVAEACGIDPIRAEELLSQCNREAKTAI
                     VVQKTGISPDEARLILSQNDGFVEKSIESVLSRKDRNSK"
BASE COUNT          308 a          354 c          249 g          334 t
ORIGIN      
        1 gaaggaaagg aaatattaat atctttttat catttaaaga tgatcaattc aaaaaaaggt
       61 tcattctccc tatttccgga agaatagaac ctttaagata agaaaacttt ctaaagattc
      121 acttcatttg gaatttcgat cttttcgcga taaaacgctc tcaatcgatt tttccacaaa
      181 accatcgttc tgcgacaaaa tcaatctggc ttcatcggga gatattcctg tcttctgaac
      241 aacaatagct gtttttgctt cacgattgca ttgagagagc agttcttcgg ccctgattgg
      301 atcgattccg caagcttcgg ctacaatccg gcgttgccga tcaatcagtt tttgattgct
      361 cggctgcaca tccaccatca ggttgctgta cgttttcccc aaccgtacca tcacagctgt
      421 ggaaagtgtg ttcagaacca ttttctgtgc cgtcccggct ttcatacggg tcgatccggt
      481 caaggcttcc ggcccgacca cggggagaat agcgatatca caaaatcgga taatcttccc
      541 gtccggattg caggcaaccc cgatcgttaa agctttccgc gattgagcct cttccaatgc
      601 acctaacaca tacggggtat ttccacttgc cgcgataccg atcacacaat ctttttctgt
      661 cacgtttaat tcaatgatct cggacgctcc caagaccggg tcatcttccg cattttcaat
      721 cgatttcgtt aaggcatccg atcctccggc aattttcccg atcacccaat ccggagaaac
      781 accaaaagtc ggtaatactt ccgacgcatc cagtaccccc aaccgaccgg aggtcccggc
      841 accgatataa atcatcctcc cgccttgttt cattcgtgaa acgatagcat cgaccgccgc
      901 tgcaatctga ggcaaaaccg ccgcaacagc gccggcgact ttttgatctt cctcattgat
      961 ttcctgaacc aacgcaagag tactcacccg atccatatcc gctgtcgccg gattgcgcgc
     1021 ttcggtagga ccgatattct cagagatatt ttctcgtttc ggaggcgccc aggtgatatt
     1081 ccccataatg accggatgtt ttgcacccgt cgccgaagga atatttccga tccgacggtg
     1141 ccaagtttcg tatgccatta acgcgaatgc cgtcgcctcc ttggcatccg aagcaattcc
     1201 caactcctca atgcgcatca ccttcgaagg cttcagccgt tcggc
//
LOCUS       DAOSMM010000182         1239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_112306_length_1239_cov_21.200169, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000182 DAOSMM010000000
VERSION     DAOSMM010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112306_length_1239_cov_21.200169"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1239
                     /locus_tag="PK898_10805"
     CDS             <1..>1239
                     /locus_tag="PK898_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20557.1"
                     /translation="KKKSAQAETENKAASYETLYQDDFSDETTGWYIKQDILDSGTYT
                     YSLTDGTYRWEYELKQGAYRYILLPVKMNLPDDGFRISVDIQFESAFSGTDAGIIFNF
                     QDKDNFYFAKLDTQGIASIYAKENGKWVLLSEKIQSEKFDPSGFNNMTVVYDTGNYEI
                     RINDIPTVRLADSRFHGGTVALIMEGNENTNPTTRFDNLTIMKKKSAQAETENKADSY
                     ETVYQQDFTEQAADWNFEPMQGDWGVINFSVTDGTYRCEMNLNKGQLRLLFPNPPINV
                     PKDGYQISVDIQLESSCPGCGPGILFSYQENGNFYFAGIDPKGTVAVYAKEDKWTPLS
                     DNVPIKDFDPSRKNKLTVINDGDNYEIRVNDISLIHITDSRFRGGVFGLLIQGKENTN
                     PTTRFDNLTILKKKSAQAETE"
BASE COUNT          399 a          269 c          263 g          308 t
ORIGIN      
        1 aagaaaaaat ccgctcaagc cgagaccgaa aataaagcgg cttcttacga gaccctctat
       61 caggatgatt tttcggatga aacaacgggt tggtatatta agcaagatat attggattca
      121 ggcacatata cctattcctt gacagacgga acctatcgct gggaatatga attgaagcaa
      181 ggtgcttaca gatatattct cctccctgta aagatgaatt taccggatga tggctttcga
      241 atcagcgtcg acatccaatt tgaatcggct ttcagtggaa ctgatgcggg aatcatattc
      301 aacttccagg acaaagacaa tttctacttc gcaaaattgg atacccaagg catcgccagt
      361 atctatgcca aggaaaacgg gaaatgggta ttgctcagcg aaaaaatcca aagcgaaaaa
      421 tttgatcctt ccggcttcaa taatatgacc gttgtctatg acacgggtaa ttatgagatc
      481 cgaataaatg atatcccaac cgttcgtctg gcggacagcc gttttcatgg gggaaccgtc
      541 gctttgatta tggaaggaaa tgaaaataca aatcccacca cccgctttga taatctcacc
      601 atcatgaaga aaaaatccgc tcaagccgag accgaaaata aagcggattc ttacgaaacc
      661 gtttatcagc aggattttac ggaacaagca gcggattgga attttgaacc gatgcaagga
      721 gattggggag taatcaactt ttccgtcaca gacggaacct atcgctgtga aatgaatttg
      781 aataaaggtc aattaagact gttgtttccg aatccgccca tcaacgttcc taaggacggt
      841 taccaaatca gtgtcgatat ccaacttgaa tcgagctgcc ccggttgcgg tcccggaatc
      901 ctgtttagtt accaagagaa cggtaacttc tatttcgccg gaatagatcc gaagggaacc
      961 gtcgctgttt atgccaaaga ggataaatgg acaccgctca gtgacaatgt cccgattaaa
     1021 gattttgatc catcccggaa aaataaatta accgttatca atgacgggga taattacgaa
     1081 attcgagtta atgatatctc tttgattcat ataacagaca gccgtttccg gggaggcgtt
     1141 ttcggattgc tcattcaagg aaaagaaaat acaaatccca ccacccgctt tgataatctc
     1201 accatcttaa agaaaaaatc cgctcaagcc gagaccgaa
//
LOCUS       DAOSMM010000183         1224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_114372_length_1224_cov_13.314799, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000183 DAOSMM010000000
VERSION     DAOSMM010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114372_length_1224_cov_13.314799"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PK898_10810"
     CDS             <1..455
                     /locus_tag="PK898_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU20558.1"
                     /translation="LSDEKDEVYHLKEQKANLTEENLSFLKRWPDTWEKDINGLRIHF
                     VHGTISDPLTGTGYENSEFANFDQIGLDVLFIGQTHRPWIRRNKHTLIVNVGSVGLPR
                     DYGNQPSFVLFDTKTKAVSIWRVEIDPGPILDHPEGIHPSVLDVLKRK"
     gene            459..>1224
                     /locus_tag="PK898_10815"
     CDS             459..>1224
                     /locus_tag="PK898_10815"
                     /inference="COORDINATES: protein motif:HMM:NF009402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOU20559.1"
                     /translation="MTDKIRVMVTGVGGGGNGEQLLKALKLAETPYHLIGADITPLSK
                     GLYMADERVILPPAVDPDYISVLTDTCKKNRVQVLFTGSEPELKRISGFQDEFRAEGI
                     FLPMNSKSVIDLCLDKSKTMSFLKENGFYFPQTWTIRNSEDLQSVDLFPLVLKPSVGG
                     GGSANSFIAQDKKELELFGCWMLNIYSEFIVQQYIGDADSEYTVGVLCDMEGNLINSI
                     AVKRNILSALSNRLKIRSRYTGEMLVLSNGITQGEIG"
BASE COUNT          335 a          231 c          316 g          342 t
ORIGIN      
        1 cattatcgga tgaaaaagat gaggtttatc atctgaagga acaaaaagcg aatcttacgg
       61 aagaaaacct ctcctttctc aagcgatggc cggatacttg ggagaaagac atcaacggat
      121 tgcgaattca ttttgtacat ggcacgattt ccgatccgct gacagggacc ggatatgaaa
      181 attcggaatt tgcgaatttt gatcaaatcg ggttggatgt tcttttcatc ggacaaactc
      241 atcgtccctg gatacggcga aataaacata ccttgattgt caatgtcggt tcagttggct
      301 tgccgcggga ttacgggaat caaccttctt ttgtcttgtt tgacacgaag acgaaggcag
      361 tctcgatctg gcgggttgag atcgatcccg gtcccatttt ggatcatccg gaaggaattc
      421 atccttcggt attggatgtt ttaaaaagga aataacgcgt gacggataaa atcagagtaa
      481 tggttacggg tgtcggcggt ggcggtaacg gcgaacaatt attgaaagct ttaaagctgg
      541 ctgagacacc ttatcatctg atcggtgcgg atataacgcc tttatccaaa gggttgtata
      601 tggcggatga acgggttatt cttccgccgg ctgtcgatcc cgattatatt tccgttctga
      661 cggatacctg taaaaaaaac cgtgttcagg tattgtttac cggatcggag ccggagttaa
      721 aacggatcag cggatttcag gatgaatttc gagctgaggg tatctttttg ccgatgaatt
      781 cgaaatccgt tatcgatctt tgtctggata aatcaaagac gatgtcattc ctgaaagaaa
      841 acggttttta ttttccgcaa acatggacaa ttcgaaattc ggaggattta caatccgtgg
      901 atctattccc gttggttttg aaaccttcgg tgggaggagg cggatcggct aacagtttta
      961 tcgcacagga caaaaaggaa ttggagcttt tcggttgctg gatgttaaac atttattcgg
     1021 aattcatagt tcagcaatat atcggtgatg ccgacagcga gtatacggtc ggtgtgcttt
     1081 gtgatatgga agggaatctg atcaattcga ttgccgtaaa aaggaatatt ttatccgcat
     1141 tgagcaaccg gctgaaaatc cgcagccgct ataccggaga gatgctggtg ctcagtaatg
     1201 gcattacaca gggtgaaatc gggc
//
LOCUS       DAOSMM010000184         1223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_114488_length_1223_cov_10.461473, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000184 DAOSMM010000000
VERSION     DAOSMM010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114488_length_1223_cov_10.461473"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK898_10820"
     CDS             <1..695
                     /locus_tag="PK898_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU20560.1"
                     /translation="MPCVAKKFEARRPELSRNGHPDVDAVITTSELAKMIKAAGIVFD
                     ELSEESFDIPYGFSTGAAVIYGMTGGVATAVVREVRYLLTGERTSDLETEPVEGFPGL
                     MRSSVTLPDGTEVKLGIVSGMANVQKVINAIDSKELEFDIVEVMACPGGCVGGGGQPI
                     PNETEQRAKRRKGIITDDIRKDLRVAHDNVLVGELYNRWLKTPNSEIAHEYLHTHYQK
                     RPRDYEITKTKN"
     gene            complement(785..>1223)
                     /locus_tag="PK898_10825"
     CDS             complement(785..>1223)
                     /locus_tag="PK898_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU20561.1"
                     /translation="TGIEGSRISMVFQENRLCRHLSGLCNCMLPLKSTEENRKRVRNL
                     LFEVGLDAMAVEKPVFELSGGMQRRVAIIRALAADSDIIFMDEPLKEMDENTEKLVVQ
                     CIRRESAGKTLILSTHNVEDAERFSANLIYLESPFINNHSEKN"
BASE COUNT          349 a          268 c          275 g          331 t
ORIGIN      
        1 tgatgccttg cgttgcgaag aaatttgaag cccgccgccc cgaattaagt cggaacggtc
       61 atccggatgt cgatgccgtt attactactt cggaactggc aaaaatgatt aaggcggccg
      121 gaatcgtttt cgatgagctg tccgaagaat cttttgatat cccctatggc ttttcaaccg
      181 gcgccgcggt catttacggt atgaccggcg gagtcgcaac agctgtggtt cgggaagttc
      241 gctacctttt gaccggagaa cgaacaagtg atttggagac ggagccggtc gaaggattcc
      301 ccgggttgat gcgttcatcg gtcactttac ctgacggcac ggaggtaaaa ttgggaatcg
      361 tcagcgggat ggcaaatgtt caaaaagtca tcaacgccat tgactcgaaa gaattggaat
      421 ttgatattgt tgaagtgatg gcctgcccgg gtggttgtgt gggaggcgga ggacagccga
      481 ttccaaacga gactgaacaa cgggcaaaac gtagaaaagg gatcatcaca gatgatattc
      541 gtaaagattt acgggttgct catgataatg tgctggtcgg cgaactgtat aacagatggc
      601 tgaagacacc caacagcgaa atcgctcatg aatatctaca tacccattat caaaaaagac
      661 cgcgagacta cgaaataacc aaaacaaaga attgatttgc tctttagaaa tataaagagg
      721 agagatcttt acggagaatc tctcctcttt ttaataatcg gtattcaagc catgaaaggg
      781 gatttcaatt tttttctgaa tgattattaa taaatggact ttctaaataa atcaaattgg
      841 ccgaaaatcg ttctgcatct tcaacattat gagtcgacag aataagagtt ttaccggcgg
      901 attctctcct gatgcactgg acaacaagtt tttctgtatt ttcatccatt tctttcaacg
      961 gttcatccat aaaaattatg tcggaatctg cagctaatgc tcgaatgatc gcaactcggc
     1021 gttgcatccc accgcttaat tcgaaaaccg gtttctcaac cgccattgca tccaatccca
     1081 cttcgaataa taaattccga actcttttcc ggttttcctc tgttgatttc aatgggagca
     1141 tacaattgca caaaccactc agatgccggc ataatcgatt ttcctgaaaa accatcgata
     1201 ttctgcttcc ctcgatcccg gtt
//
LOCUS       DAOSMM010000185         1221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_114766_length_1221_cov_9.908233, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000185 DAOSMM010000000
VERSION     DAOSMM010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114766_length_1221_cov_9.908233"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PK898_10830"
     CDS             complement(<1..652)
                     /locus_tag="PK898_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU20562.1"
                     /translation="MNCYERVMNRLAGKPVDRIPNLCILMTFAAKYIHVPYDQFVQDY
                     RLLVEGNIRCCEDFKIDLLSAISDPAREASDLGAAIAFPVDDVPHPTVPFVSQSADLA
                     KLKLVKPEDGRRMSDRLAAVSLYREKAGKEYPVMGWVEGAFAEACDLDSVSNIMISVV
                     DDPEFTAELLEFCTQQAILFAEAQVDAGADFIGIGDAAASLISPRMYRKWAMPYEKR"
     gene            complement(842..>1221)
                     /locus_tag="PK898_10835"
     CDS             complement(842..>1221)
                     /locus_tag="PK898_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOU20563.1"
                     /translation="TYEILPEEVEVRAVAHEGFVVASEGSNIAALVTTLTPELEREGL
                     SREFVRRVQDLRKTADLDISDRIDVVYEASSELAEAIRENAGYISNETLAVSLEEGPV
                     PADWASVEDSFDGQTVKVGLRKA"
BASE COUNT          355 a          331 c          225 g          310 t
ORIGIN      
        1 tccgtttttc atacggcatc gcccattttc gatacattcg cggactgatc agagaagcag
       61 cggcatcccc gattccgata aaatccgccc ctgcatcaac ctgtgcctct gcaaaaagga
      121 ttgcctgctg agtacagaat tcaagcaatt cagcggtaaa ttccggatca tctaccacag
      181 agatcatgat attactaacc gaatccaaat cacaagcttc ggcaaaagcg ccttcaaccc
      241 agcccattac cggatattcc ttccccgctt tttctctgta aagagaaaca gcagctaatc
      301 gatcggacat tctgcgcccg tcttcgggtt tgaccaattt taattttgcc aaatcagctg
      361 attggctgac gaaaggaact gtcggatgag ggacatcatc aaccggaaat gctattgcgg
      421 ctcccaaatc cgatgcttct ctagccggat cggatattgc ggagagcaaa tcgattttaa
      481 aatcttcgca acaacgaata tttccttcaa ctaaaagacg atagtcttgc acaaattgat
      541 catagggaac atgaatatat tttgcggcaa aagtcatcaa aatacacaaa ttcggaatcc
      601 gatccaccgg cttgccggcc agtcgattca tcacccgctc ataacagttc ataagatttt
      661 cctcccttgc ttatcatact cacccataga aaaaattgca attgatcata aaacggatcg
      721 aaaaaaattt ctgaaatatt ttcccgacgg taacaattgc gaaccatttc aatgggtatt
      781 cgatcagaaa atacgaaaga ggggtaaaaa acccctcctt cgaacctgat cacaacattc
      841 tttatgcttt ccttaatcca accttgaccg tctgtccgtc gaaagaatct tcaaccgacg
      901 cccaatccgc gggaaccgga ccttcttcta aagacacagc cagcgtttca tttgaaatat
      961 aacccgcatt ttcccgaatg gcttccgcca gttcactgct ggcctcatag accacatcga
     1021 ttcgatcgga aatatccaaa tcagccgtct tgcgcagatc ctgaacccgg cgtacaaatt
     1081 ctctcgatag cccttctctt tccaactcgg gtgtcaaagt tgtcaccaag gcggcgatat
     1141 ttgacccttc cgaagcgacg acaaaacctt cgtgagcgac ggctcgaact tccacttctt
     1201 cgggaagaat ttcataagtc t
//
LOCUS       DAOSMM010000186         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_115041_length_1219_cov_8.640034, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000186 DAOSMM010000000
VERSION     DAOSMM010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115041_length_1219_cov_8.640034"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PK898_10840"
     CDS             complement(<1..1160)
                     /locus_tag="PK898_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU20564.1"
                     /translation="MIHQHFMLVPTQTVTENILLGLDEPKFIMNLSKYDEIIRQLGIE
                     NNLIVDPTAYIWQLSVGEQQRVEILKMLYRGCDILIMDEPTAVLAPQEIDVLFNTLRS
                     MTEKGKSIIFISHKLNEILEIADRVTVLRRGKVTAADMPIEGVTKEELASLMVGREVI
                     FNIDKSACHAGEPVLIVENVYAENDKGLPALKGVSLQVCAGEIVGIAGVAGNGQRELA
                     EVIAGLRKIQSGSIRSKDLDLTKVPIKKAIEEGIAYIPEDRHHVGTAPNLSVVDNLIM
                     KNYDQCPISKGIGMDEGAARAYAESLKGEYDIIVPSIDTPVRLLSGGNLQRVILAREL
                     SNMKSLLIAVQPTRGLDVGAIEGIQRMLLKQRESGGGVLLISEELEELVSLS"
BASE COUNT          326 a          302 c          229 g          362 t
ORIGIN      
        1 tcgctcaaac taaccaattc ctccagctct tcggagatta atagaacgcc gcctccggat
       61 tctcgttgct tgagcaacat tctctgaatt ccttcgatgg ctccgacatc caaaccacgg
      121 gtcggttgaa ccgcgattaa aagtgacttc atattggaaa gttctcgcgc taaaataacc
      181 cgttgcaaat ttcctccgga aagaagcctt actggtgtat cgatcgacgg aacaatgata
      241 tcgtactcgc cttttaagct ttcagcataa gctctggcag cgccttcgtc catgccgatt
      301 cctttgctga tcgggcactg atcatagttt ttcataatta agttatccac aacactcaag
      361 ttgggagcag tccccacatg atggcgatct tcgggaatgt aggcgattcc ttcttcaatc
      421 gctttcttaa taggtacttt tgtcagatcc aaatctttgg agcggataga cccgctttga
      481 attttccgta aaccagcgat aacctcagcc aattcacgtt gaccgtttcc cgcaacacct
      541 gcgattccga caatttcacc ggcacaaact tgcaaggaaa ctcctttcaa cgcaggcaat
      601 cctttatcat tttcggcata aacattttcc acgataagaa ccggttcgcc agcatggcaa
      661 gccgacttat cgatattgaa aatcacttca cgaccaacca tcaaagatgc caactcttct
      721 ttcgtaacac cttcgattgg catatcggcg gctgttactt taccacgtcg taaaaccgta
      781 actcgatcgg caatttcaag aatttcattc aatttgtgac taataaaaat aatcgatttc
      841 cctttttccg tcatggatct taaggtgttg aataatacat caatttcttg aggcgcaaga
      901 acagcggtgg gttcatccat aatcagaata tcgcatccgc ggtaaagcat ttttaggatt
      961 tccactctct gttgttctcc aaccgacaac tgccagatat atgctgtagg atcaacaatc
     1021 aaattgtttt caattccaag ttgccggatg atttcgtcat atttactcag attcatgatg
     1081 aatttgggct catccaatcc taagagaata ttttcggtta ccgtttgagt cggaacaagc
     1141 ataaagtgct gatggatcat tccaattccg gctttgattg catcacgtgg ggatctgaat
     1201 ctgacctgtt gtcctttta
//
LOCUS       DAOSMM010000187         1169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_122532_length_1169_cov_13.177738, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000187 DAOSMM010000000
VERSION     DAOSMM010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122532_length_1169_cov_13.177738"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1169)
                     /locus_tag="PK898_10845"
     CDS             complement(<1..>1169)
                     /locus_tag="PK898_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU20565.1"
                     /translation="ISWLDENDRLVIRTSTQVPFHVRRIISPLIDVPVQNIRVIKPRI
                     GGGFGAKQEVLLEDLCALLTLRTGRPVRFYFDRQDEFRSSRSRHPDTIRFKVGVTKSG
                     RIKAIEMDMIENTGAYGVHGFTVCNVTGTRGLSTYRADSMRFHADIVYTNLPVPGAFR
                     GYGGPQGIYGIECIIDEVAEKLKMDPIKFRMKNVVRPGEKIKIMDKLAEFDPVPQKVN
                     SYGLDACIRKGMEAIDWNRRKSKQFHSDPSKPNICRGIGMSIMMHGTAIAGIDMGAAA
                     MKINDDGSFNLNVGATDLGTGSDTILAQMAAEVLKIPVSKIVVYSSDTDFTPFDVGAY
                     ASSTTYISGMAVKKCAEKIKEQVLERAALMLQQPKDDLYLKDEKVYAPNGDHVTM"
BASE COUNT          295 a          299 c          253 g          322 t
ORIGIN      
        1 tccatggtaa cgtgatcccc attcggagcg taaacctttt catctttcag atacagatcg
       61 tctttcggct gctgcagcat caaggcggct ctttccagaa cctgttcttt aattttttcg
      121 gcacattttt tgactgccat cccggaaata taggtggtac tggatgcata agcgcctaca
      181 tcaaacggcg taaaatccgt gtccgaagag tagacaacga tttttgagac gggaattttc
      241 agcacttccg ctgccatttg tgccaatatc gtatccgaac cggttcccag gtcggtcgct
      301 ccgacattca aattgaaaga accgtcgtca ttaatcttca tcgctgcggc gcccatatca
      361 attccggcaa tggcggtccc atgcatcata atggacatcc cgatccctct gcagatattc
      421 ggcttcgagg gatccgaatg gaactgtttg gatttcctgc ggttccaatc gatcgcttcc
      481 atccctttcc gaatacaggc atccaatccg tagctgttaa ctttttgcgg gaccggatcg
      541 aattctgcca gtttatccat aattttgatt ttttcaccgg gacgaacaac atttttcatc
      601 cggaatttaa tcggatccat ttttaatttt tcggcgactt cgtcgataat gcattcgatc
      661 ccgtaaattc cttgcggtcc gccataaccg cgaaaggctc cggggaccgg taaattggta
      721 taaacaatat ccgcatggaa tcgcatgcta tctgcccgat atgtggataa cccgcgtgtt
      781 ccagttacat tacagacagt aaaaccatgc acgccgtaag ccccggtatt ttcgatcata
      841 tccatttcaa tcgctttaat gcgtccggat ttcgtaacgc cgaccttaaa acgaatggta
      901 tcgggatgac gcgaacgaga ggagcggaat tcatcttggc gatcgaaata aaatcggacg
      961 ggccttccgg ttcgcagcgt taacaacgca cataagtctt caagtaaaac ttcctgtttt
     1021 gcgccgaatc ctccgccgat ccgcggttta atgacacgga tattttgaac cggaacatcg
     1081 atcaaaggag aaataattct ccgaacatgg aaaggaacct gtgtgctggt acggattacc
     1141 aaacgatcgt tttcatccag ccagctgat
//
LOCUS       DAOSMM010000188         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_127388_length_1140_cov_8.954839, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000188 DAOSMM010000000
VERSION     DAOSMM010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127388_length_1140_cov_8.954839"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PK898_10850"
     CDS             complement(<1..1105)
                     /locus_tag="PK898_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU20566.1"
                     /translation="MISRREVFIGTYKRHAVDLHLKMGFQKDGTFQALSSMAYLDTGA
                     YAGLGPAVMGLFSEHLGGPYVIPNVKIDSYLVYTNKAPAHAMRGFGAPQGAFATESLI
                     NRAADILHIDPIEIHMKNALTQGAVGTLGQKMEHVIGLREALEAVRDSDLWKEKDTNQ
                     DPSVGFGIAAGYLSCGLGKGVPDSAKVEIDREPNGDFTVRVGLVDIGQGNATALAAIA
                     GEALKVPLEKIRLIMADTAQTFDCGSTAGSRSVFIAGNAILAAVQDYFSHPETGRGLG
                     EAEFPQSKTDLNVIGFPHAMYTFVAQAVKLKLDPISGQPQLAGIFAATEAGKVINRLS
                     MDGQIQGGIAMSIGYTLGENMNYRNGIPDNQRFT"
BASE COUNT          291 a          297 c          253 g          299 t
ORIGIN      
        1 cggtaaatct ttgattatcc ggaatcccat tccgataatt catattctcg ccgagcgtat
       61 agcctatgga catcgcaatt ccaccttgta tttgcccgtc cattgagagc cggttaatta
      121 cctttcccgc ttccgtagct gcaaaaatac ccgctaattg cggttgcccg ctgatcggat
      181 ccaattttaa tttgacagcc tgggcgacaa aagtatacat ggcatgagga aaaccaatga
      241 cattcaaatc cgttttcgat tgcggaaatt cagcttcacc caatcctctc ccggtttccg
      301 gatgggaaaa gtaatcttga acagcggcaa gaattgcatt cccggcgata aagaccgatc
      361 gggaaccggc tgtggatccg caatcgaaag tctgtgctgt atcagccata atcaggcgga
      421 ttttttctaa ggggactttc aatgcttcgc cggcaatcgc agccaaagcg gttgcgttgc
      481 cctgtccgat gtcaaccaat ccgactcgaa cggtaaaatc tccgtttggt tctcgatcaa
      541 tctccacttt tgccgaatcg ggaacgcctt tccctaatcc gcaactgaga taaccggctg
      601 cgatcccaaa gccgacagaa ggatcttgat tggtatcttt ttctttccat aaatccgaat
      661 ctctgaccgc ctccaatgcc tcccgaagac cgataacatg ttccattttt tgaccgaggg
      721 ttccgacggc accttgggta agtgcattct tcatatggat ttcaatcgga tcgatatgaa
      781 gaatatctgc ggcccgattg atcaaacttt cggtggcaaa tgcaccctga ggagcaccga
      841 agccacgcat agcgtgagcg ggtgctttgt tagtataaac gagataagaa tctatcttaa
      901 cattcggaat aacgtacgga ccgcctaaat gttcactgaa gagccccatc accgccggac
      961 cgaggcctgc ataagcgccc gtatccaaat aagccatact ggagagagct tggaaggtgc
     1021 cgtctttttg aaagcccatt tttaagtgta aatctacggc gtgtctttta tacgttccga
     1081 taaaaacttc tcgtcggctg atcacaattt tcgccggtct tccggtacgg taggcaacca
//
LOCUS       DAOSMM010000189         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_127390_length_1140_cov_5.297696, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000189 DAOSMM010000000
VERSION     DAOSMM010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127390_length_1140_cov_5.297696"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1140
                     /locus_tag="PK898_10855"
     CDS             <1..>1140
                     /locus_tag="PK898_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU20567.1"
                     /translation="VAYRTGRPAKIVISRREVFIGTYKRHAVDLHLKMGFQKDGTFRA
                     LSSTAYLDTGAYAGLGPAVMGLFSEHLGGPYVISNVKIDSYLVYTDKAPAHAMRGFGA
                     PQGAFATESLINRAANILQVDPIEIRMKNALTQGALGTLGQKMEHVVGLREALEAVRD
                     SDLWKEKNTNQDPSIGFGIAAGYLSCGLGKGVPDSAKVEIDREPNGDFTVRVGLVDIG
                     QGNATALAAIAGEALKVPLEKIRLIMADTTQTFDCGSTAGSRSVFIAGNAILAAVRDY
                     FSHPETGRGFAETEFPQSKTDLNVIGFPHAMYTFIAQAVKLKLDPISGQPQLAGIFAA
                     TEAGKVINRLSMDGQIQGGIAMSIGYTLGENMNYRNGIPDNQRFT"
BASE COUNT          311 a          256 c          284 g          289 t
ORIGIN      
        1 tggttgccta ccgtaccgga agaccggcga aaattgtgat cagccgacga gaagtgttta
       61 tcggaacgta taaaagacat gccgtagatc ttcacttgaa aatgggtttt caaaaagacg
      121 gaaccttccg agctctctca agtacggctt atttagatac gggcgcttat gcaggtctcg
      181 gtccggcggt gatgggactc ttcagtgaac atttaggcgg tccgtacgtt atttcaaatg
      241 ttaagataga ttcttatctc gtttataccg acaaagcacc cgctcacgct atgcgtggct
      301 tcggagctcc tcagggtgca ttcgccaccg aaagtttgat caatcgtgcc gcaaatattc
      361 tccaagtcga tccgattgaa atccgcatga agaatgctct aactcaaggc gctctcggga
      421 cactcggtca aaaaatggaa catgtcgttg gccttcgaga agcattggaa gcggtcagag
      481 attctgattt atggaaagaa aaaaatacca atcaagatcc atctatcggt tttggaatcg
      541 cagccggcta tctcagttgc ggattaggaa aaggcgttcc cgattcggca aaagtggaga
      601 ttgatcgaga accgaacgga gattttaccg ttcgagtcgg attggttgac atcggacagg
      661 gcaatgcaac cgctttggct gcgattgccg gcgaagcact gaaagtcccc ttggaaaaaa
      721 tccgcttgat tatggcagac acaacacaga ctttcgattg cggatccaca gccggctccc
      781 gatcggtctt tatcgccggg aatgcaattc ttgctgctgt tcgagattat ttttcccatc
      841 cggaaactgg gagaggattc gctgaaactg aatttccaca gtcgaaaacc gatttgaatg
      901 tcatcggttt tcctcacgcc atgtatacct ttatcgcaca ggctgtcaaa ttaaaattgg
      961 atcccatcag cgggcaaccg caattagcgg gtatttttgc agctacggaa gcaggaaagg
     1021 taattaatcg tctctcaatg gacgggcaaa tacaaggtgg aattgcgatg tccataggct
     1081 atactctcgg cgagaatatg aattatcgga atgggattcc ggataatcaa agatttaccg
//
LOCUS       DAOSMM010000190         1132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_128759_length_1132_cov_8.714020, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000190 DAOSMM010000000
VERSION     DAOSMM010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128759_length_1132_cov_8.714020"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..>1132)
                     /locus_tag="PK898_10860"
     CDS             complement(183..>1132)
                     /locus_tag="PK898_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOU20568.1"
                     /translation="LLEYSHHIRKLIDFDTIAEAKDLRIVVDSMYGSGRGVIRNLLGG
                     TGVEVNEIRAELNPGFGGVHPEPIYRNLGALASALAAGLGNLGLATDGDADRIGAMDE
                     RGNFIDPHKIMALSIKYLHEKRGLSGALVRTVSTTRMIDCLGKKYGLEVFETPVGFNH
                     ISDHMMERDVLIGGEESGGISFKGHIPEGDGIIMGLLITEMVAAYHATLGELVRQLED
                     EIGAFYYDRTDLRLKHPVAKDEMIKSLVNNAPASIGGETIQQISTLDGVKYIMADDSW
                     LLIRPSGTEPVLRVYAEGRSPQMVKELLGFGNSVAETVK"
BASE COUNT          310 a          308 c          224 g          290 t
ORIGIN      
        1 ggaaaggcat ggattcggtt tcaaagaaaa ccatagtaaa tatcctgaat aatttcctta
       61 aaaagtttaa tattacaaag aaacctgtcc aataaactgt ctttttcaag caatacaatc
      121 aaaacgagtc cgattttcta tggaacaaat cggactcgtc gggttaaatt tatctataag
      181 cgctatttta ccgtttcggc aaccgaattc ccgaagccga gcaattcttt gaccatctgc
      241 ggcgatcgac cttcggcgta cactcgcaag accggttccg ttccggacgg gcgaattaat
      301 aaccaagaat catccgccat gatgtacttg acgccatcca gcgtgctgat ttgctgaatc
      361 gtctcgccgc cgattgaagc gggtgcgtta tttaccaacg acttaatcat ttcatccttt
      421 gccacggggt gcttgaggcg taaatctgtc cgatcgtaat agaaagctcc gatttcatcc
      481 tcaagctgcc ggaccaattc acccaatgtc gcatggtaag cggcaaccat ttctgtgatt
      541 aacaatccca taatgatacc gtcaccttcc ggaatatgac ccttgaacga aattccgccg
      601 gattcttcac cgccgattaa cacatcccgc tccatcatat gatcggaaat atgattgaaa
      661 ccgaccggtg tctcaaagac ttccaatccg tattttttac ctaagcaatc gatcatccgg
      721 gtggtagaaa ccgttcgtac caatgctccg ctcaagccgc gtttttcatg gagatactta
      781 atcgagagtg ccatgatttt atgaggatca ataaaattcc cccgttcatc catcgcgccg
      841 atccgatccg catccccatc cgtagccaac cccaaatttc ccaacccagc cgctaaggcg
      901 ctggcaagcg cacccaaatt acggtaaatc ggctccggat gaacccctcc gaaacccgga
      961 ttcaattcgg ctcggatttc attgacttcc acgccggtcc cgcctaacag attacgaatc
     1021 actccgcgac cggaaccgta catggaatcc acaacgatcc gcaagtcttt tgcttcggcg
     1081 attgtgtcaa aatcaattaa ttttcgaata tgatggctgt attcgaggag ag
//
LOCUS       DAOSMM010000191         1114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_131965_length_1114_cov_8.694051, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000191 DAOSMM010000000
VERSION     DAOSMM010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131965_length_1114_cov_8.694051"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1114)
                     /locus_tag="PK898_10865"
     CDS             complement(<1..>1114)
                     /locus_tag="PK898_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20569.1"
                     /translation="EPLLADGPIVWEDDYNIIVKIKPVAHWSDGEPLTADDVVFTFNS
                     LDPDVIGYQTQWSDVWSYLEKVEKVDDLTVRFTIPAEPYNVHMLPSYLADTRIFPKHI
                     WEPLFEGELKETTAKQEYFNENPIGSGPFKLKFYDETRIVLERDDNYWGQDPSMFGKL
                     TEVKYFIHPIFESNEAGNLAFAQGEIDVSQQFIPSIWDIQKQLVDENGKSLVTTYYPE
                     APYQLGYSMPSLVINMTKPGLDDFTVRKAIALSLDFEAIGTNAMSGYTQPIEISLFNN
                     YLFKDYLDYDDEEMLNLMWDTTDLDGNRATANAMLDEAGYKDIDGDGMREMPDGSKIE
                     WKAMAPAGWSDWNMSLEILSESAKAIGLNIITDFPE"
BASE COUNT          277 a          262 c          250 g          325 t
ORIGIN      
        1 cattcgggga aatcggtaat gatattcaag ccgatggctt ttgccgattc gctcaaaatt
       61 tcgagagaca tgttccaatc tgaccatccg gcgggagcca ttgccttcca ttcgatcttg
      121 ctgccgtccg gcatttctct catgccgtcg ccgtcgatat ctttataacc ggcttcgtcc
      181 aacatggcgt tggcggttgc gcggtttccg tctaaatcgg tggtatccca catcaggttg
      241 agcatctctt catcgtcgta atccaagtaa tctttgaaga gatagttgtt gaagaggctg
      301 atttcaatcg gttgcgtgta tccgctcatg gcattggtac cgatcgcttc aaaatcaagt
      361 gataacgcga ttgcttttcg gacggtgaag tcatccaagc cgggtttcgt catgttgata
      421 accaacgatg gcatactata gcccagctga taaggagctt ccggataata tgtggtgact
      481 aaggatttcc cgttttcgtc caccagttgc ttttgaatat cccatatact cggaataaat
      541 tgttgggata catcaatttc accttgggca aatgccaggt ttccggcttc gttgctctca
      601 aaaatcgggt gaatgaagta tttaacttcg gtgagtttcc caaacatcga aggatcttgg
      661 ccccaataat tgtcatcccg ttccagtacg attctggtct cgtcatagaa tttcaactta
      721 aacggaccgg aaccgatcgg attctcattg aaatattcct gtttcgcggt ggtttctttc
      781 agttcgcctt caaataaggg ttcccaaatg tgcttcggga aaatacgggt atccgccaaa
      841 tagctgggga gcatatgcac attgtatggc tcggcgggga ttgtaaagcg aaccgtcaga
      901 tcatccactt tttccacttt ttcaagatag gaccatacat cggaccattg agtctgatat
      961 ccgatcacat cgggatcaag cgaattgaaa gtaaacacca catcatccgc ggttaatggc
     1021 tcaccatctg accaatgtgc gacgggtttg atcttaacaa taatgttata atcatcttcc
     1081 cagacgatag gaccatccgc taataatggt tcat
//
LOCUS       DAOSMM010000192         1114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_131969_length_1114_cov_6.491974, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000192 DAOSMM010000000
VERSION     DAOSMM010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131969_length_1114_cov_6.491974"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1114
                     /locus_tag="PK898_10870"
     CDS             <1..>1114
                     /locus_tag="PK898_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU20570.1"
                     /translation="EPLLADGPIVWEDDYNIIVKIKPVAHWSDGEPLTADDVVFTFNS
                     LDPDVIGYQTQWSDVWTYLEKVEKVDDLTVRFTIPAEPYNVHMLPSYLADTRIFPKHI
                     WEPLFEGELKETTAKQEYFNENPIGSGPFKLRFYDETRIVLERDDNYWGQDPSMFGKL
                     TEVKYFVHPIFESNEAGNLAFAQGEIDVSQQFIPSIWDIQKQLVDENGKSLVTTYFPE
                     APYQLGYSMPSLVINMTKPGLDDFTVRKAIALSLDFEAIGTNAMSGYTQPIEISLFNN
                     YLFKDYLDYDDEEMLSLMWDTTDLDGNRATANAMLDEAGYKDIDGDGMREMPDGSKIE
                     WKAMCPAGWSDWNMSLEILSESAKAIGLNIITDFPE"
BASE COUNT          328 a          231 c          261 g          294 t
ORIGIN      
        1 atgaaccatt attagcggat ggtcctatcg tctgggaaga tgattataac attatcgtta
       61 agatcaaacc cgtagcccat tggtcagatg gtgagccatt aaccgctgac gatgtagttt
      121 ttactttcaa ttcacttgat cccgacgtga tcggatatca aactcagtgg tccgatgtat
      181 ggacatatct tgaaaaagtg gaaaaagtgg acgatcttac tgttcgtttc acaattcccg
      241 ccgagccgta caatgttcat atgctcccca gttatttggc agacacccgc attttcccga
      301 agcacatttg ggaaccctta tttgaaggtg aattgaaaga aaccacagcg aagcaggaat
      361 atttcaatga gaatccgatc ggttccggtc catttaagtt gagattctat gatgaaacca
      421 gaattgtgct ggagcgggat gataactatt ggggtcaaga cccttcgatg tttgggaaac
      481 tcacagaagt taaatatttc gttcacccga tttttgaaag taatgaagcc ggtaacctgg
      541 cgtttgccca aggcgaaatt gatgtatccc aacagttcat cccaagcata tgggatattc
      601 aaaagcaact ggttgatgaa aacggaaaat cgttagtgac cacttatttc ccggaagctc
      661 cttatcaatt gggttatagt atgccttcgt tggttatcaa catgacaaaa ccaggcttag
      721 atgatttcac cgtccgtaaa gcgatcgcgt tatcgttgga ttttgaagcg atcggtacca
      781 acgccatgag cggatataca caaccgattg aaatcagcct cttcaacaac tatctattca
      841 aggattacct ggattatgat gatgaagaga tgctcagtct gatgtgggat accaccgatc
      901 tggacggaaa ccgagcaacc gcgaatgcga tgttggacga agctggttac aaagatattg
      961 acggcgacgg tatgagagaa atgccggatg gaagtaagat cgaatggaaa gcaatgtgcc
     1021 cagccggatg gtccgattgg aatatgtccc ttgaaatcct gagcgaatcg gcaaaagcca
     1081 tcggcttgaa tatcattacc gatttccccg aatg
//
LOCUS       DAOSMM010000193         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_134786_length_1099_cov_3.470307, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000193 DAOSMM010000000
VERSION     DAOSMM010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134786_length_1099_cov_3.470307"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK898_10875"
     CDS             <1..396
                     /locus_tag="PK898_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU20571.1"
                     /translation="RSQLIDITTRIQEVVTASGIREGLCTVFIPHTTAAVTINEDADP
                     DVIHDFLYELDKIVPWQDGYRHMEGNSAAHLKSSLIGVSEQILIENSRLILGTWQGIY
                     FCEFDGPRTRRVLVRIDSSDFSTRNTAAS"
     gene            658..>1099
                     /locus_tag="PK898_10880"
     CDS             658..>1099
                     /locus_tag="PK898_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU20572.1"
                     /translation="MKKGLSIVLSFIMIVSLFIVSVPVSAQDAVKLVVWESTGGPDEW
                     IKAAGEAFTKLNPNITVEFVNVELGDTSGQIALDGPAGVGPDLFAAPHDKLGELVIGG
                     HILPTVNPDAVKEAVLASTSQALTYDGVMYGYPVSAETYALYYNK"
BASE COUNT          301 a          255 c          239 g          304 t
ORIGIN      
        1 cgttctcaat taatcgatat aactactcgg atccaggaag tggttacggc ttccggtatc
       61 cgagagggac tctgtaccgt cttcattcct cacaccaccg ccgccgttac catcaatgag
      121 gatgctgatc cagacgtgat ccatgatttc ttatacgagt tggacaagat cgtaccatgg
      181 caggacggat accggcatat ggaaggcaat tccgccgctc acttgaaatc ctccctgatc
      241 ggcgtctccg aacagatcct tatcgaaaat agccgattaa tactgggaac ctggcaggga
      301 atttattttt gcgaattcga cggaccgcgc acgcgtcgcg tactggttcg aatcgattca
      361 tccgattttt caacccgaaa tacagccgca tcctaattca ctccgtcacc tgccatatcg
      421 tgattcctca ggccgatctg aggagttttt atatcttcta tccttcttta aacatgcaat
      481 cggatattca aataaatcat ttattcgata tataatttaa atagctgaaa tcggataaaa
      541 cacataataa agtgttctta tcggaacggt caaatcaaat tcgctgtcca aactatatta
      601 accggaataa gttcgatcat ttccggaata atcatttgaa ttcagaaagg ggaaaaaatg
      661 aaaaaaggtt tatcgattgt actttcgttc atcatgattg tttcactttt tattgtttct
      721 gtcccggtat cggctcaaga tgccgtaaaa ctggtagttt gggaatctac cggcggaccg
      781 gatgaatgga ttaaagctgc cggagaggca ttcaccaaat tgaaccccaa catcaccgtt
      841 gaatttgtca atgtggaatt gggagacacc tctggccaaa ttgctttaga cggaccggct
      901 ggagtcggac cggatttgtt cgcagcgccg catgacaaat tgggagaatt ggtcatcgga
      961 ggtcatattc tcccgacggt aaacccggat gcggtaaaag aagccgttct tgcttcaacc
     1021 tctcaggcac ttacctatga cggggtcatg tacggttatc cggtttcggc tgaaacctat
     1081 gcgctttact ataacaaag
//
LOCUS       DAOSMM010000194         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_139457_length_1075_cov_10.750980, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000194 DAOSMM010000000
VERSION     DAOSMM010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139457_length_1075_cov_10.750980"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PK898_10885"
     CDS             <1..619
                     /locus_tag="PK898_10885"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20573.1"
                     /translation="VAITGKRIKDSWDTHYKDPLDFYDLKGITEVTAKGLHISGLRFE
                     PAECAYMHPGKCAAVYAGEEKLGILGELHPAIHKEYDLLSSPIILLDLNLEKMEPLSE
                     TIYKVHSISPFPAILEDIAVVVDEKITAGEVENVIRQAGGKLLQSVELFDIFRNEQIG
                     AGKKSLAYSLKYQSDEKTLTDSDATAVRNKIIKRLDQVLGAKLRS"
     gene            772..>1075
                     /locus_tag="PK898_10890"
     CDS             772..>1075
                     /locus_tag="PK898_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20574.1"
                     /translation="MKSILRKSILYKSGISSLDYAMNPVLGCAHGCRYPCYAWLMAMR
                     FGKVKSYSQWIQPAIVGNTLELLEKELRKWHWKIHHVQLCLTTDPFMVGYPEVRQLS"
BASE COUNT          306 a          218 c          265 g          286 t
ORIGIN      
        1 ggtcgcgatt accggaaaac gtataaaaga ttcttgggat actcactata aagatccttt
       61 ggacttttac gatctcaaag gcattacgga agtgacggca aagggtctgc acatttccgg
      121 tttgcgattt gagccggctg aatgcgctta tatgcacccg ggaaaatgcg cagcggttta
      181 tgccggggag gaaaaactgg gtatccttgg tgaattacat cccgctatcc ataaagaata
      241 cgatctttta tcttcaccga tcattctgtt ggatctgaat ttggaaaaaa tggagccgtt
      301 atccgagaca atctataaag tacattcgat ttcgccattc ccggcgattc ttgaagatat
      361 agcagtcgtc gtcgatgaaa aaatcaccgc gggcgaagtg gaaaatgtga tcaggcaagc
      421 cggcggaaag ttgctgcaaa gcgttgaatt atttgatatt ttccgcaatg agcaaatcgg
      481 agccggaaaa aagagcttag cttacagtct gaaatatcaa tcggacgaaa agacattaac
      541 cgacagcgat gccacagccg ttcggaacaa gattattaaa cgattggatc aggttttggg
      601 cgcgaagctg cggagctgaa tgctctttcg gcaatccgac cggttcgtgc ctttcgagtg
      661 agggatcata gaacagtacg ggaaatttat ccgtactgtt ctatattaga atcccccgac
      721 gttttcacgg gtattgaaaa tatcaccggc ggttcgaatt acaattaaaa aatgaaaagt
      781 attctcagaa agtcaatctt atataaatcc ggtatatcat cgctggatta tgcgatgaat
      841 ccggttttgg gatgtgccca cggttgcaga tatccttgtt atgcctggct gatggcgatg
      901 cgtttcggaa aagtcaagag ttacagccaa tggattcaac cggctattgt cggcaatacc
      961 cttgaactgt tggaaaaaga gcttcggaaa tggcattgga agattcatca tgttcaactc
     1021 tgtctgacga cggatccttt catggtcgga tatccggaag tccggcagct ttcgt
//
LOCUS       DAOSMM010000195         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_139461_length_1075_cov_6.620588, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000195 DAOSMM010000000
VERSION     DAOSMM010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139461_length_1075_cov_6.620588"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PK898_10895"
     CDS             <1..619
                     /locus_tag="PK898_10895"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20575.1"
                     /translation="VAITGKRIKDSWDTHYKDSLDFYDLKGITEVTAKGLHISGLRFE
                     PAECTYMHPGKCAAVYAGDEKLGILGELHPAIHKEYDLLSSPIILLDLNLEKMEPLSE
                     TIYKVHSISPFPAILEDIAVVVDETITAGEVEAVIRQAGGKLLQSVELFDIFRNEQIG
                     AGKKSLAYSLKYQSDEKTLTDSDATAIRNKIIKRLDQVLGAKLRS"
     gene            772..>1075
                     /locus_tag="PK898_10900"
     CDS             772..>1075
                     /locus_tag="PK898_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20576.1"
                     /translation="MKSILRKSILYKSGISSLDYAMNPVLGCAHGCRYPCYAWLMAMR
                     FGKVKSYTQWIQPAIVSNTLELLEKELQKWHWKIRHVQLCLTTDPFMVGYPEVRQLS"
BASE COUNT          307 a          213 c          261 g          294 t
ORIGIN      
        1 ggtcgcgatt accggaaaac gtataaaaga ttcttgggat actcactata aagattcctt
       61 ggacttttac gatctcaaag gcattacgga agtgacagca aagggtctgc acatttccgg
      121 tttgcgattt gagccggctg aatgcactta tatgcacccg ggaaaatgcg cagcggttta
      181 tgccggggat gaaaaactgg gtatccttgg tgaattacat cctgctatcc ataaagaata
      241 cgatctttta tcttctccga ttattctgtt ggatctgaat ttggaaaaaa tggagccgtt
      301 atccgagacg atctataaag tacattcgat ttcgccattc ccagcgattc tcgaagatat
      361 tgcagtcgtc gtggatgaaa caatcactgc gggagaagtg gaagctgtga tcaggcaagc
      421 cggcggaaaa ttgctgcaaa gcgtcgaatt atttgatatt tttcgcaatg agcaaatcgg
      481 agccggaaaa aagagcttgg cttacagcct gaaatatcaa tcggatgaaa aaacactcac
      541 cgacagtgat gccacagcca tccggaataa gatcattaag cgattggatc aggttttggg
      601 ggcgaagctg cggagctgaa cgctctttcg gcaatccgac cggttcattc ttttcgagtg
      661 agggatcata gaacagtacg ggaaatttat ccgtactgtt ctttattaga attccccgac
      721 gttttcacgg gtgttgaaaa tatcaccgac ggttcgaatt acaattaaaa aatgaaaagt
      781 attcttagaa agtcaatctt atacaaatcc ggcatttcat cattagatta tgcgatgaat
      841 ccggttttgg gatgtgctca cggctgcagg tatccttgtt atgcctggtt gatggcgatg
      901 cgtttcggga aggtcaaaag ttacacacaa tggattcaac cggcaattgt cagtaatacg
      961 cttgaattgt tggaaaaaga actgcagaaa tggcattgga agatccgtca cgttcaactt
     1021 tgtctgacga cggatccttt catggtcgga tatccggaag tccggcagct ttcgt
//
LOCUS       DAOSMM010000196         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_147201_length_1039_cov_17.428862, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000196 DAOSMM010000000
VERSION     DAOSMM010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147201_length_1039_cov_17.428862"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PK898_10905"
     CDS             <1..684
                     /locus_tag="PK898_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOU20577.1"
                     /translation="NLCYETLEGIAKHETEFNFSDLLEYNPDSRGHLEAQIANVADEI
                     AYSAHDLDDGLRSGIITVDMCEDLLIWKTIAEEIHWVGPKLADLDRHRFINALINLAV
                     TDTFYTSQEKITSAKIKSVDELQRLPYNVVGNSESWLIQNRELKDFLFNNLYRHYRVV
                     RMNLKADMIITRLFEAFRKEPSLLPKHIQTTIEERGTDTAICDYIAGMTDRYAIEQYQ
                     KLFEPSLLP"
     gene            696..>1039
                     /gene="greA"
                     /locus_tag="PK898_10910"
     CDS             696..>1039
                     /gene="greA"
                     /locus_tag="PK898_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU20578.1"
                     /translation="MPTETSYLTSEGAQKLQEELEFLKGPRRAELAKRLRFAIEQGDL
                     SENADYSAAKEEQAFVEGRIQELETILSNVKIIDDLKREKGVVNLGSTVRIQEEDFEP
                     EEYKIVGPQEADP"
BASE COUNT          321 a          196 c          250 g          272 t
ORIGIN      
        1 aatctttgtt atgaaacctt ggagggaatc gccaagcatg agacggagtt taatttctcg
       61 gacctgctgg aatataatcc ggattcgcga gggcatctgg aggctcaaat tgccaatgtc
      121 gccgatgaaa tagcttattc cgctcatgat ttggatgatg gcttacgctc gggaatcatc
      181 acggtagata tgtgcgagga tctgctgatt tggaaaacaa tcgcggagga aatccactgg
      241 gtgggtccaa aattggcaga tttagacagg catcgtttta ttaatgcatt aatcaatctg
      301 gctgtcacgg acaccttcta tacctcacag gaaaagatta cttccgcgaa gattaaatca
      361 gtggacgagt tgcaacgttt gccttataat gtagtgggta atagtgaatc ctggttgatt
      421 caaaaccgtg agctgaaaga ttttcttttt aacaacttgt atcgtcatta tcgagttgtc
      481 agaatgaatt tgaaagcgga tatgatcatt accaggttat ttgaggcttt ccgtaaggaa
      541 ccttcacttt tgccgaaaca tattcaaact acaattgagg agaggggaac agacaccgcc
      601 atctgcgact acatcgcagg gatgactgat cgatatgcta tcgaacaata tcagaaatta
      661 tttgaaccct ctctcctgcc ataaaggagt ataaaatgcc tacagaaaca tcctatttaa
      721 cttcggaagg tgctcaaaaa cttcaggaag aactcgaatt tttaaaagga ccccggcggg
      781 ctgaacttgc caaacggttg cgttttgcga ttgaacaggg agatctctct gaaaatgcgg
      841 attattcggc agcaaaagag gaacaagctt ttgtcgaagg aagaatccaa gagcttgaaa
      901 caatcttatc caatgtaaaa attatcgacg atttgaagcg ggaaaaagga gtggtcaact
      961 tgggttcgac ggtgagaatt caggaagaag atttcgagcc ggaagaatat aaaattgtcg
     1021 gaccgcagga agcggatcc
//
LOCUS       DAOSMM010000197         1011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_153876_length_1011_cov_10.385983, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000197 DAOSMM010000000
VERSION     DAOSMM010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153876_length_1011_cov_10.385983"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..>1011
                     /locus_tag="PK898_10915"
     CDS             11..>1011
                     /locus_tag="PK898_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU20579.1"
                     /translation="MLNEMQNAKMIYKLKRCLTVFEPYVFRTIDRLPYRIYQTKEHVR
                     SVPDDALFSDPGEKWGGEWMNCWFKGSYTPRPESAGQTLYVKPSVGGYEGLLWINNEP
                     RGAFATKIVVTRHGNHFCSPICRKADPAETIELAIEFYAGHYVIGCLPFETAERGDFM
                     YPCGPIEICEKVWDVADFICDLRVLIQLVEMSDEKSFRRAEIMNVLTEVHRVILYSPE
                     DAEYDDWIDSLRQARSIMQPALAAKNGTNAPVAALTGHSHMDTAWLWPVNETIRKNAR
                     TIANQLSMLEYYPEYRFIQSASLHSRMIEQNYPQIFSVMKEMIARGRYEVNGGVWVE"
BASE COUNT          266 a          213 c          267 g          265 t
ORIGIN      
        1 gaagaatttt atgctcaatg aaatgcaaaa tgccaagatg atctataaat tgaaacgatg
       61 tctgacggtt ttcgaaccat atgttttcag aacgatcgat cgtcttcctt atcgaattta
      121 tcaaacaaaa gaacacgtcc gctcggttcc ggatgatgcg ctgttctccg atccgggtga
      181 aaaatggggc ggggaatgga tgaactgctg gtttaaaggc agctacacgc cacggcccga
      241 atcagccgga caaacgcttt atgtgaaacc ttcggtgggg ggatatgagg gactgctgtg
      301 gattaacaat gagccgcgcg gagcgtttgc gaccaaaatt gtggtgaccc gccacggaaa
      361 tcatttttgc agtcctattt gtcgaaaagc cgatcctgcc gaaacgatcg aattggccat
      421 cgaattttat gccggccatt atgttatcgg atgtttgcct tttgaaaccg ctgaacgagg
      481 cgatttcatg tatccgtgcg gaccgattga aatctgtgaa aaagtatggg atgtggcaga
      541 ttttatctgt gatcttcgcg tattgattca attggtggaa atgtcggatg aaaaatcttt
      601 tcgccgggcg gaaatcatga atgtattgac ggaagttcat cgcgtgattt tgtattctcc
      661 cgaggatgcc gaatacgatg actggatcga ttctctccgg caggctcgtt cgatcatgca
      721 gccggcgctt gccgcaaaaa acggaacaaa tgctccggtg gcggcattga ccgggcattc
      781 ccatatggat accgcgtggt tgtggccggt caacgagacg attcgaaaaa atgcccgaac
      841 tattgcgaat caactcagca tgttggaata ttatccggaa tatcgattta ttcaaagtgc
      901 ttcgctgcat tcccggatga tcgaacaaaa ttatcctcag attttttccg tgatgaagga
      961 aatgatcgcc cgcggacgat acgaagtgaa cgggggtgtc tgggtcgaat g
//
LOCUS       DAOSMM010000198         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_155636_length_1004_cov_16.287671, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000198 DAOSMM010000000
VERSION     DAOSMM010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155636_length_1004_cov_16.287671"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1004)
                     /locus_tag="PK898_10920"
     CDS             complement(<1..>1004)
                     /locus_tag="PK898_10920"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOU20580.1"
                     /translation="EFAIELLEKLGYESVYGPDIAPDNEFSTSFGATSDKRESYEQVL
                     LNNRLRKALKRINRNMPADVLEDAIKEIQRIHSPELLTNNETFHRFLTEGIPVSKRVD
                     GEDRGDLVWLIDFKNPYNNDFLVVNQFTVVENNHPKRPDIVLFVNGIPLVVIELKNAA
                     DENASIRMAFKQIETYKALIPTLFTYNGFVVISDGLEAKAGTISSGFSRFMAWKSSDG
                     QVEASHLVSQLETLIEGMLNKETLIDLMRHFIVFEKTKKEDPVTGIITMNTVKKMAAY
                     HQYYAVNRAVESTLRAAGYTASSRASLSLPSRASGSEIALVSEVLEDTVSLEGSVRES
                     "
BASE COUNT          274 a          237 c          206 g          287 t
ORIGIN      
        1 cgattctctg acactgcctt cgagagaaac ggtgtcttcg agaacctcag acaccagagc
       61 aatttcgctg cctgaggctc tcgaaggcag cgaaagagag gctctcgaag acgctgtata
      121 tcccgctgcc ctcaacgtcg attcaacagc ccggttcacc gcatagtatt gatggtaggc
      181 agccatcttt ttaacggtgt tcatggtaat aattcccgtt acgggatctt cttttttcgt
      241 tttttcaaaa acaatgaaat gacgcattaa gtcgatcaac gtctccttat tgagcattcc
      301 ctcaataagg gtttccaact gactgaccaa atgcgaggct tcaacttgcc cgtcggatga
      361 tttccatgcc ataaaacggc tgaatcccga agaaatggtt cccgctttgg cttccaaccc
      421 atcggaaata accacaaacc cgttgtaagt gaaaagcgtt gggatcaacg ctttataagt
      481 ctctatttgt ttgaatgcca tacgaatgga tgcattttca tcagcagcgt ttttcagttc
      541 gatgactacc aacggaatgc cgttaacaaa caaaacaata tcgggtcgtt ttggatggtt
      601 gttttccaca acggtaaact ggttaacaac caaaaaatcg ttgttatacg gatttttaaa
      661 atcaatcagc caaacaaggt cgccccgatc ctcgccatcc acccgctttg aaacaggaat
      721 accttcggtt aaaaaccggt gaaaggtttc gttattcgtc aacaattccg gtgaatgaat
      781 acgctggatt tcttttatgg catcttccag tacatcggcg ggcatgtttc tattgatacg
      841 ttttagtgct tttcgcaacc gattgttcaa taaaacctgt tcgtagcttt cccgtttgtc
      901 ggacgtagca ccgaatgagg ttgaaaattc attgtcgggt gcaatatcag gaccataaac
      961 cgattcgtaa ccaagttttt ccagtaactc gatagcaaat tctt
//
LOCUS       DAOSMM010000199         1003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_155876_length_1003_cov_17.706751, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000199 DAOSMM010000000
VERSION     DAOSMM010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155876_length_1003_cov_17.706751"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..959
                     /locus_tag="PK898_10925"
     CDS             234..959
                     /locus_tag="PK898_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOU20581.1"
                     /translation="MPIIQIEGADLGYRTDAEPALKDITISFYPGDMVAVIGPNGAGK
                     STMMKSIVGLIPPLKGKVNVHGELHGAHPDCVSYIPQRESIDWNYPITVREVVMMGRY
                     GKIPFYKSPSEKDQKTVEDALKRMEITALADRKIRDCSGGQQQRVFLARSLAQEPHIL
                     LMDEPFNAVDLATEKIILDNLRVFKERHVTTLVATHDLSLVSENFEKVLLLNKRIIAY
                     GNREEVLTDKKLQLAYGKKAIYF"
BASE COUNT          286 a          201 c          254 g          262 t
ORIGIN      
        1 cagagcgttt ttaattcttc cagaatccgt tcttcctcag cggaaataac gatcattaat
       61 gtcgcttttg cgcgcagata aaggtcaatt tcatttaaaa aatccatgaa aaaaattata
      121 ctatagggac gacagcgaga taaaaccgac cgtcatcgaa gtgaagtgac caatccgata
      181 aaggtgattg aaattcaagt acaattcagc ctatgaacat tgatctgagt caaatgccca
      241 tcattcaaat tgagggggct gatctgggat accggacgga tgcggagccg gcattgaagg
      301 atattacaat cagtttttac ccgggtgata tggtggctgt gatcggtccg aacggtgccg
      361 ggaaatcgac gatgatgaaa tcgatcgtcg gattgatccc gccgttgaag gggaaggtca
      421 acgtccacgg ggaattgcat ggtgcgcacc ccgattgtgt ttcctatatt ccgcagcgcg
      481 agtcaatcga ttggaattat ccgatcacgg ttcgggaagt cgtcatgatg ggccggtatg
      541 ggaagattcc tttttacaaa agcccttccg aaaaagatca aaagacagtc gaggacgcct
      601 tgaagcggat ggaaattacc gcattggcgg accggaaaat tcgggattgt tccggcggac
      661 aacagcaacg cgtttttttg gcacgatcgc ttgcgcagga accccatatt ctgttgatgg
      721 acgaaccgtt taatgccgtg gatttggcaa ccgagaaaat tattttggat aatttgcgtg
      781 tttttaaaga gcgccacgtg accacgttgg ttgctactca tgatctttcg ctcgtttcgg
      841 aaaattttga aaaagtgttg ttgctgaata aacggatcat cgcttatgga aacagggaag
      901 aagttctgac cgataaaaag ctgcaactcg cttacggaaa gaaagcgatc tatttctaag
      961 gaattacgat ggcagtttta ctggaaccgt tccaatatga ttt
//
LOCUS       DAOSMM010000200         1002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_2397
            NODE_156105_length_1002_cov_9.126716, whole genome shotgun
            sequence.
ACCESSION   DAOSMM010000200 DAOSMM010000000
VERSION     DAOSMM010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864464
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:48:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,143
            Genes (coding)                    :: 2,137
            CDSs (with protein)               :: 2,137
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156105_length_1002_cov_9.126716"
                     /isolate="AMR_MDS_2397"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..>1002
                     /gene="pyrB"
                     /locus_tag="PK898_10930"
     CDS             293..>1002
                     /gene="pyrB"
                     /locus_tag="PK898_10930"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOU20582.1"
                     /translation="MTTSLFGKDILDLQLLSDDEIRLIMDTTARYEKVMQNGGRLMNL
                     YGKTLATLFYEPSTRTRLSFETAMLKMGGSVISVADAKTSSSAKKGETVYDTGKMIES
                     YSDVAVIRHPVTGSAKELADGSDKPVINAGDGAGQHPTQALLDLYTILKEKGKIEGLT
                     LCLAGDLKNGRTVHSGAALFARMGAKFIFTAPEALAMPDEITESLRKKGVEIEIIPDL
                     MQAVKKADVLYMTRIQQER"
BASE COUNT          314 a          198 c          235 g          255 t
ORIGIN      
        1 atcctaatcg gcttgtctga caaataacgt ttactatatt aaaatataat cattaacttt
       61 ttcgtataat aaaaatctat cgcccaaatc ttgcaagggc atttgaaagg ctggtaaaat
      121 aagtggtgtt aaattcaaga aatttatttt gatgatggaa tgccgccgca atgaaatgag
      181 ccgaaaatcg gaatttcata aagatttgac tgcgcaaata tcacaaaata tgccaagaat
      241 tggtaattaa tttacattta ttattcataa aggaaaaata aggagaaaaa gtatgacaac
      301 atcattgttc ggtaaggata ttcttgatct tcaactgctt tctgatgatg aaatcagatt
      361 gatcatggat acgacggcca gatatgaaaa ggtgatgcaa aacggcggtc gtctgatgaa
      421 tttatacggg aaaacattgg caacgctctt ctatgaacct tccactcgga cgcgactttc
      481 atttgaaacc gcgatgctga aaatgggcgg atcggtcatt tccgtcgccg atgccaaaac
      541 atcaagttcc gcgaaaaaag gtgaaacggt ttacgacacc ggaaaaatga ttgaaagcta
      601 ttcggatgtc gcggtcattc gccatcccgt aaccggttcg gcgaaagaat tggcggacgg
      661 ttcggataag ccggtcatca acgccggtga cggagccggt cagcacccga cacaagctct
      721 actggatctt tataccattc ttaaagaaaa gggcaagatc gaagggttga cgctttgctt
      781 agccggtgac ctcaagaacg gtcgaacggt tcattccgga gccgccttat ttgcccgtat
      841 gggtgctaaa tttattttca cggcaccgga agcattggct atgccggacg aaattacgga
      901 aagtctgcga aagaagggtg ttgaaatcga gatcattccc gatttgatgc aggcggtgaa
      961 gaaagcggac gtcctctata tgaccagaat ccagcaggag cg
//
